Document 1gVm9B0MVgGzG1nzLOdbE1baK

AR23-Q3H0 ACUTE TOXICITY TO AQUATIC INVERTEBRATES - Ceriodaphnia dubia TEST SUBSTANCE___________________________________________ Identity: N-Ethylperfluorooctanesulfonamlde; may also be referred to as U1464, EtFOSA, EtPOSA, F-6309, or FX-12. (1Octanesulfonamide, 1,1,2,2,3,3,4,4,5,5,6,6,77,8,8,8heptadecafluoro-N-ethyl-, CAS # 4151-50-2) Remarks: Material is a white solid. Sample purity and lot number were not recorded. METHOD Method: Adapted from OECD 202 - Water accommodated fractions Type: Static acute GLP: No Year completed: 1998 Species: Ceriodaphnia dubia Analytical monitoring: Nominal concentrations only. Temperature, pH and dissolved oxygen recorded Statistical methods:: EL50 (lethal loading) values were calculated using the probit method. The NOEL was calculated by Fisher's Exact Test, TOXSTAT Software Package, Version 3.5, Laramie, Wyoming. Test daphnid source:. AScI in-house culture, Duluth, MN Test daphnid age at study initiation: < 24-hour old neonates Test conditions: Dilution water: Carbon-treated dechlorinated City of Duluth, MN tap water aerated for 24-hours prior to use in the test. Dilution water chemistry during the study: Hardness 40-42 mg/L as CaC03 DO: 8.5-9.3 mg/L Alkalinity: 42 mg/L as CaC03 pH: 7.8-8.0 Conductivity 118-125 pmhos/cm Test Temp. Range: 21.2-21,3 Stock and test solution preparation: Test solutions prepared by mass addition of test substance to 1.5L aliquots of dilution water (one per concentration). The solutions were vigorously stirred (vortex 1/3 to Vz solution depth) for 20 hours. The aqueous phase was siphoned from the vessel at mid-depth. 006063 Exposure vessels: 30 mL borosilicate glass beakers Exposure volume: 20 mL Number of replicates: four Number of daphnids per replicate: five Number of concentrations: Five plus a negative control Element basis: mortality RESULTS___________________________________________ Nominal loading concentrations: Bk control, 78,130, 216, 360 and 600 mg/L Element value: 24-hour EL50: 380 (330-438) mg/L 48-hour EL50: 328 (278-391) mg/L 48-hour ELi0: 184 (130-224) mg/L 48-hour NOEL: 216 mg/L Mortality of controls: 0% after 48-hours exposure Remarks: All values reported are for nominal concentrations. No measurement of test concentrations was conducted. Percent Mortality Nominal Loading Concentration, mg/L 24-Hours 48-Hours Negative Control 78 130 216 360 600 0 0 0 10 25 100 0 0 5 20 40 100 Remarks: All values reported are for nominal loading concentrations. No measurement of test concentrations was conducted. CONCLUSIONS The test sample 48-hour ELsofor Ceriodaphnia dubia was determined to be 328 mg/L with a 95% confidence interval of 278-391 mg/L Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_____________________________________________ 006064 Reliability: Klimisch ranking 3. This study lacks analytical data for determination of the test substance concentration in the test solutions and determination of the sample purity. Testing was also done well above the solubility limit of the test substance with the use of water accommodated fractions. REFERENCES____________ ___________________________________ Study conducted at the request of 3M Company by, AScI Corporation, Duluth, MN. OTHER____________________________________________________ Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/18/00 006065 AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 STUDY TITLE ACUTE TOXICITY OF U1464 TO CER10DAPHN1A DUBIA DATA STANDARD ADAPTED OECD GUIDELINE 202 STUDY COMPLETED May 4, 1998 TESTING FACILITY AScI Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811-5712 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUMBERS AScI Study ID# 5010-068 3M Company Lab Request# U1464 Sponsor: 3M Company Sponsor Study ID# U1464 1 006066 AScI Corporation/AScl-Dululh Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Manager Testing Facility Director Definitive Test Dates Data Validation and Report Review Retention of Raw Data and Final Report Location of Raw Data and Final Report Acute Toxicity of U1464 to Ceriodaphnia dubia. Adapted OECD Guideline 202. 3M Environmental Technology and Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. Rochelle R. Robideau, 3M Environmental Technology and Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. Tel No. (612) 778-7065. U1464. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Todd McGuire. Joe Amato. Donald Mount. April 15-17, 1998. Alan Mozol. J^1H /Report Signatured < Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Sponsor: 3M Company Sponsor Study ID# U1464 2 006067 AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID# 5010-068 2.0 OBJECTIVE(S) To determine the 48-h EL's (effective loading's) EL10 and EL50, and 48-h NOEL (no observed effect loading) of the test substance, U1464, for Ceriodaphnia dubia. This study was conducted according to the AScI study plan retained by the Sponsor and an adaptation of OECD Guideline 202 (OECD 1993). Test solution preparation was wateraccommodated fraction (WAF) per Girling & Whale 1994. 3.0 TEST METHODS Test Substance Test Organisms Dilution Water Exposure Chambers Properties'. U1464, white crystals, specific gravity 1.7 (water = 1), stable, and negligible solubility in water. Test loadings: 0 (dilution water control), 78, 130, 216, 360, and 600 mg/L. Each test solution prepared by mass addition of test substance to 1.5-L aliquots of dilution water. Each solution was vigorously stirred (vortex 1/3 to 1/2 of flasks' depth) for 20 hours to obtain WAF's. Test solution obtained from flasks by siphoning aqueous phase from mid-depth. Source: Healthy, <. 24-h old Ceriodaphnia dubia neonates obtained from AScI individual organism cultures maintained in synthetic moderately hard reconstituted water (MHRW) (hardness 80 to 100 mg/L as CaC03). Use in test: Gravid adults isolated in MHRW 24 h before test initiation. Used 5 organisms/replicate and 4 replicates/test loading. Handled with a smooth-bore eye dropper. Did not feed during the test. Source: Carbon-treated dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis of post carbon (PC) water is in the Raw Data Package. Characteristics: At test time, the water had a hardness of 40 mg/L (as CaC03) and a pH of 7.87. Aerated for 24 h prior to using in the test. Type: 30-ml borosilicate glass beakers. to Use in test: 20 ml of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Sponsor: 3M Company Sponsor Study ID# U1464 3 006068 AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID# 5010-068 Incubation Observations Endpoint Calculations Duration: 48 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white fluorescent bulbs. Temperature: 21 C. Biological: Daily- counted dead test organisms. Did not remove any daphnids from the exposure chambers at 24 h. Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, pH, DO, and conductivity. All determinations were made according to APHA methods (1995). EL10andEL50: EPA Probit Method. NOEL: Fisher's Exact Test (TOXSTAT statistical software, Version 3.5, University of Wyoming & West Inc.). 3.0 RESULTS Test Substance Toxicity (95% Confidence Interval) Pertinent Data are in Table 1 Ranges of Water Chemistry Parameters 24-h ELS0: 380 mg/L (330-438 mg/L). 48-h EL50: 328 mg/L (278-391 mg/L), 48-h EL10: 184 mg/L (130-224 mg/L) 48-h NOEL: 216 mg/L. Total hardness (as CaC03 mg/L): 40-42. Alkalinity (as CaC03 mg/L): 42. Temperature (C): 21.2-21.3C. DO (mg/L): 8.5-9.3. pH: 7.8-8.0. Specific conductivity (iimhos/cm): 118-125. Sponsor: 3M Company Sponsor Study ID# U1464 4 006069 AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 4.0 TEST QA/QC QA/QC Criteria Test organism dead, immobilized or trapped in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. Test duration must be 48 h. Reference substance toxicity test (this test is optional). Data 0% dead, immobilized or trapped. The lowest DO concentration measured was 96% of the air saturation value at 21.0C. The test duration was 48 h. Current control chart for C. dubia acute testing is included in the raw data package. 5.0 REFERENCES American Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Girling, A.E., G.F. Whale, and D.M.M. Adema. 1994. A Guideline Supplement for Determining the Aquatic Toxicity of Poorly Water-Soluble Complex Mixtures using WaterAccommodated Fractions. Chemosphere, Vol. 29, No. 12, pp. 2645-2649. Organization for Economic Cooperation and Development (OECD). 1993! OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. University of Wyoming & West Inc. 1996. Toxstat Version 3.5. Cheyenne, Wyoming 82001. USEPA Probit Analysis Program. Version 1.4. Sponsor: 3M Company Sponsor Study ID# U1464 5 006070 M I COPIAVAUM AScI Corporation/AScl-Duluth Environmental Testing Division Submitted to 3M Co. - S/9S AScI Study ID# 5010-068 TABLE 1. ACUTE TOXICITY O F U1464 TO CERIODAPHNJA DUBIA Te$t Substance Loading (mg/L) 0 78 130 216 360 600 V .<. . . Cumulative Number and Permit o f Dead Organisms* 48 h. 0(0) 0(0) 0(0) 2(10) 5(25) 20 (100) 0(0) 0 (0) 1(5) 4(20) 8(40) 20 (100) * Percentages are in parenthesis. EFFECT AND NO-EFFECT LOADINGS WITH CORRESPONDING 95% CONFIDENCE INTERVALS EL's and NOEL 24-h EL50 48-h EL50 48-h EL10 48-h NOEL Data (mg/L) 380 328 184 216 95% Confidence Interval 330-438 278-391 130-224 N ot Applicable Sponsor: 3M Company Sponsor Study ID# U1464 6 006071