Document 1g33JqpYL9k32GbpBjrRz5vRd

fm -o o ^ i BIODEGRADATION F 7 ^J5 - TEST SUBSTANCE Identity: Perfluorooctanesulfonate, DEA salt; may also be referred to as PFOS DEA salt, FC-99, or 3M Sample No. 2. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, compd. with 2,2'-iminobis[ethanol] (1:1), CAS # 70225-14-8) Remarks: Test sample is a mixture of the test substance in water (approximately 25% test substance and 75% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. METHOD Method: Not noted. Type: Aerobic GLP: No Year completed: 1979 Contact time: 20 days Innoculum: Sludge collected from an aeration basin at the municipal waste treatment plant in St. Paul, Minnesota. RESULTS 5-day BOD: 20-day BOD: COD: 44,000 mg/kg 82,000 mg/kg 78,200 mg/kg Remarks: This material has a perfluorinated moiety and a nonfluorinated moiety. Current knowledge of these chemistries suggests that all biodegradation indicated in this study is likely due to reduction of the non-fluorinated moiety, 2,2'-iminobis[ethanol]. Testing was conducted on the "as-sold" mixture of the subject material in water (-24.5% subject material). The values reported apply to that mixture and not directly to the subject material. CONCLUSIONS______________________________________________ ___ This testing indicated that the 2,2'-iminobis[ethanol] moiety is likely to be biodegraded in aerobic environments capable of supporting biodegradation. This study cannot rule out the possibility that conditions could be found that would allow for biodegradation of the fluorochemical 000264 moiety, nor could it exclude the possibility that a microorganism could eventually be enriched that could metabolically alter this chemical. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_________________________________________________ Reliability: Klimisch ranking 3. This study lacks sufficient documentation, characterization of the test substance purity, and analytical measurement of test concentrations. REFERENCES____________________________________ ' This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number 48955, 6/8/79. O T H E R ________________________________________________ Last changed: 5/2/00 000265 Form 10620-5-PWO : LAB REQUEST BOOK - 42-5W Ref: ENVIR. ASSESS. INQ. # PRODUCT ECOLOGICAL ASSESSMENT REQUEST FOR LABORATORY WORK LAB REQUEST NO. 3K13xctkm'roai PRODUCT: T C - 4 1 f L -*o^ V y* litt* i f e B ix Vfc-It/ REQUESTER Name: A V W c f f a r Request Date: ProiectNo.: (ULOO Code No.: Sample Date: .^ /y /7 Jf Phork No. Prob.No. Date Ni*ded: Description: Purpose of Tests: Pr* /* SC m. 79 LABORATORY tfcjS O /i/h Date Received Samp a: 5 ^ * 7 9 ." Analyst: Ix O . Shl~F- Date Completed: L - 4 --7* Hours Scent: LJA*~ 'J . /MTS'* O TSTPH-- n Y B 'P Please Call R e a u e & te r I f * n e la v t A re F n r o u n to PHYSICAL & CHEMICAL PROPERTIES Heatof Com bustion Cal/G (B T U /L bJ , Density Ash Other WATER POLLUTION PH COD - v * /K A bod* BOD, U ltim ate TOC / fCy, 'BOD J' 4 ' w - GC Oxygen Uptake Dehydrogenase A c tiv ity (TTCI O th e r / *7 %/7-00 7*7; 4 0 0 VY,P to \ if^ o e o M T 1%} <*00 0 Ltt-J >I.00 =v A T T A Z f t e 134t BIOASSAY Specify Organism and M ethod: ?# If . S f / ' * %i\ k se A )T -jo / -/UALYTf&Au / R BSm ~TS 1fft- 1- f f XEM#/ LEACHATE FROM LEACHING TEST PH COD B0D5 BOD, U ltim ate TOC GC Bioa'.sav Other y^v. f'i t -*uXi .-J k { l'ir , - A i. 3 S MlS ft/V Tfl he V vf L, Tl te "CY IMG, Ai OTHER B ioaccum ulatio n __ Persistence Degradation Products W hit# R eturn to R aauaattr C am rv . EnwIm nm kM al Pewve* n iflM Plie Pink - Environm ental Enora. Leb Oidanro<v ^ n t t fi ( %t en 70 > S in W ^CT J < L U -fc. V> W ^ ^^ ^ I 0 0^00 O O SO <Orv* *o^ SO O > > Q ? i ? m ? ~3 $S t r V* o O O O 0 ' O* *O . 1 8 S "* O &^ 9e OQ J* ^S ** 0 O >e O O O o O ^N o 0 0 S *0 ooO e 9 rowM iM W ir w o Bottla No. , SampleSite Itr 6V 57 t'0 y .o $ .0 2-S ' ENVIRONMENTAL LABORATORY BOO WORK SHEET Blank: Devriptlon / S f> 1(0 Depletion Data: kjM . Dilution Water: / ________ Sead: - 2 ** Bk. Avq. ___ _______________ ^ D ay BOD Avg. >/ c tr re s ' 57,000 ^ * io r ZS S '.lT 4OOO 3 g , d*w> y 1 f t s r /.s' *> <0.5* y 1^,000 . A A //a few. ' $-&>l j ) /Z- % *" -Z> > iy > , / -^neu^iofvL r \ \ f r ' , . '.v* / L 6 1 ^ i! k 1 1------------- 000268 FORM ( H I M rw e Bottla No. Samplt Siza ENVIRONMENTAL LABORATORY BOD WORK SHEET Blanks: Daacription /* * & ^ D apittion </?'/* S' Data: S W % " 7 `) k iM . S t J j Dilution Watar: 1 3 ^ ________ s**d: ftr trrfiy j ' 2 v , / f y Bk. A w g.___ _______________ / J - Day BOO Avp. nu ** 57 % Ao a .,o V* $>o x<r /S T d * >/ 7 r g>a o 1,% o 4 3 d tS * <^3r y . > -- ' _ S'Gj O o o -W /S O O *1y( 9 c o r* A A //a ^ ' - it /1 ^ t* S t i i i d ? ? ........ . * C00269 >MM I0k4 w * o ENVIRONMENTAL LABORATORY BOO WORK SHEET Date: 5 r - ( 8 ~ 7 `7 J ^. Dilution Water: / S J ________ 3ottle No. Sample Size 7<- 57 % ,0 $0 2 -< T Description F T .-1 1 'J *V . ^ Depletion /s ? > 2 -^ - Day BOD its - r .o r 8 ,So S .W %^fO S M ---------if > _ .9 7 .a a o T fo e o 7 f; aoo 4 A u J tL m P d flJ ltv tiU y J /. h h z Avd- - viC>'' ?. . a 'r . f) 1, k ^ ' /W \r f - > 000270 * l* 2 U - 4 T W O tttleNo. SampleSize 2 3 __ u 2il. -- -- -- -- 2.2* -- w-- 2- -- ENVIRONMENTAL LABORATORY BOD WOflK SHEET Blanks: Page No. Date: L).4. S^tL Dilution Water: /$? P S8* * " Z ." rJhj/ 8k. Avg. ^ ' Description / ^ Depiction v .'r r& io O tusr 0 ' * M S' 0/3 O S.1* ?.vr OA<y Ale,, p e n rT/iA/ -- i?,37^ S,'jd ^35" *.<>0 8./c / . j> S//P $"/3o STDayBOD Avg. 7?? 3/ 2 ,1 /3 yJ /7 *7 7</ 73 7J? J27 <* 3//o Iflo (-> * a u JLyuA. 3ooy+P S-S<> 7-00 H'a.O} &&> 63-70 s'-3o S f-- r<Vnr~-LA^r.^iivi V 'Yo 7i --7o7* Uf*UY*eY. S 3 .3 --6 '1f -r*-+ /^ DO AX4<^<-tW - p o b srp- /o /S V -m a a~2> V/5T>-vv*a i/ 3-70 2.70 S'-CT %no Hno s.7o 3 ,sr 2/0 'urAe 22.0 22.0 23o - ^ "Tifrr* W lls YZfVS $ el ,i- s VSfY s YZlz s `/S 'ri 5 < /.l S ft i ^ X * ' 76** ^M _ 000271 M r* )0 a ^ 4 PWO ENVIRONMENTAL laboratory BOD WORK SHEET Blanks: ottla No. 23 Gl *i<r 22 13 V . 2. Sample Size Description Depletion -- M iT *,<a O r ff -- fKJL*svu4ia J 35- 0* f< --t i r jt. 3o o.-rs- -- M o 5 2 5 " 0 '<*f tP iif -- 7X ^ *Jz jZ%J %.4o -jr .v y hr#c -- X-t^o B U n & d J ^ 7 0 ^.c? -- J5//0 ./ * Pag* N o.______ _ Data: S?/ff-7 7 J.4. S<. D ilution Water: /f f P Seed: Bk. Avfl. * * _________ 3 - Day BOD Avg. r 31H _ 3/ / ^3 *f.1 13 77 Ha. o') M o -?.Yr jAsA. 'ZOO't~f *,*> ??,&> < U o 3.7^ A ^ o ** ftTV Ca CamxV _ . 7 73 7 S: -2. to Psoh S rt> . /o /SQnna d i s t c ^ u M o <,Yo s.-io v . ? r ^ O 3/?0 B,7 o 3 <;o W 1CS < /?irs YX&s 4^72 5 y m 5m 3s </$rr3 s <. ^ ro ^ 2 ^> 25*0 2$*0 /j ' /> A1 t (rf( `c / V ' v 000272 tri* !* pwo ttle No. 23 6/ iz zz W 2- Samplt Sizt -- -- -- -- -- -- -- ENVIRONMENTAL l a b o r a t o r y BOD WOflK SHEET Blanks: Paje N o._________ Date: S * * / # - * ? *? t.O. o D ilution Water: !% P ______ Seed: Bk. Avg. * y *.- Z ^ rP i ' ________ Description Depletion ')A Day BOD M 7l&<s A 3 s*sy7*3<> t> r f %fiO 7 ./ t't s. wmmvm ^.<5o (a>Hf mo o * r * J5//0 '5 V r Av. 111. ti TS - 3.1 /3 /7 X it/ /UA. 3OOv*f 4 *,*> S '* r 3,?0- * H1.O $,*> 2,0 0 %W> -- Ll**J? 10 t'ft* *'**-1 r ri . * 73 if 7 ff . pCb srp./o /SOma br~& O jL M ^ u ^ k ^ 8,?o V ^ s' ifiQ Z,(cO %fto U r - V W its *??rs %$$ 211 s 81 S YSls 5 VtfjO s * r, J T T 6^ p -1 a n i> 0 ,_ 9- 000273