Document 1ayqjkN11Rqo4DZdDMXojgaE
1474 S.Vandhvxntbx Avb. . St. Loots 10, Mo.
Worenber 10, 1953
CERTIFICATE OF ANALYSIS
SO 3JE0T -
Tha Acuta Oral Toxioity (LD^g) of Aroolor 22$k For Rat*
TEST CONDUCTED FCR -
/-
Mon*ante Ghstdeal Coppmy, St Louis, WLsaouri* Monsanto Project Ko*i SA-15
EXPERIMENTAL PROCEDURE -
S
midHutiid Aroolor 12$h Into tha stomachs at Sprague Dawlay strain albino"
rata by mma of a rubber eathater attached to a hypodermic springs
Animals weighing 175 to 250 grwns mm used in'the test.
'
Altar the approximate Minimum Lethal Dose wo found, groups of enimala Mr fad at latels daslgnad to`blanket the .toxioity rang thereby supplying data for ealculatioa of tha LDge which was done according to a modification of the method of Bliss (Uuart. J. Pharm. and Pharmacol*, 11, 1938). The data based on results obtained from a total of ILL rata are found in Table I*
Selected animals from these recalling a lethal dose mm autopaied and macroscopic examination made of tha Ttsoera.
CONSULTANTS TO THE FOOD, CHEMICAL AND PHARMACEUTICAL INDUSTRIES
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Carttfiacfei of Aaaiyii* -- ?* i (11-10-5J)
TABL2 1
THS LDS0 OF AROGLOR 125k FC8 RAO
Animal No- Sox
V*igfa* Orawa
Doaa. Ml./K**
Fat
1- Mala
2- Mala
3- Kala
4 Ftumla
5- Famala
Mala
7- Female
8- Famala
9- Female
10- Mai
U- Mala
12- Mala
1J- Female
Hi- Fawale
15- FtMla
16- Mala
17- Mala
18- Mala
19- Mala
20- Female
21- Fair ala
22- Fair ala
23- Mala
24- Mala
25- ''ala 26- Mala /'
27- Famada . 28- Fartala/'
29- .*!/ 30- F^rnaxa
31- Feft*l 32- Femal* .
33- Mai* \
3k" M&lo
35- Mala
"
36- Female
37- Fairala
38- Famala
39- M1
UO- Mala
41- Mala
225 2.50
H5 2ii0
185
195 220
200 .
175 190
230 '
250
3*00
as \
190 / / \ '
210 \ \ / /
230 \ v /
24o \ <
2# \
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, 19? 'v. '
3.2Sv
l& \ 2IQ
'' s \ '' X
V 230\
.
215 ' / 2U5\ - /
200 '
180 '
190
\ 21S 205
190 ZtiO
3.75
230
245 180 190 185
225 210
240
5urrlw4 Died
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SurTi-rad Surrlwd Surfi-nad
Diad
\ * Diad
~ 'W
Survived
urdvtd
Sorri-rad
' D3^4
' DA4d
Survived
Survived
Surrivad
Diad i
Survived
Survived f-`
Diad
Survived
Diad
Clad
Sunlvtd
Survived
Diad
Diad
Sundvad
Diad
Diad
Diad
Survived
Diad
Diad
Survived
Diad
Survived
Clad
Diad
Diad
Diad
Diad
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TOi Mmaacrto On
Gem^miy
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Imdiip HLssoorl
SoiMiifl* AaamiMm CirtJilatto of Analyidj -- pf 3 (22-10^3)
DDCCSSIOi -
Tbs LD-- w** oalenla^^ to bo 3 10 adl1111 tort por IdJop'M with nppar and
lawsr liAta of 2.9S to 32i* aHHtor por Mlop** SurriTtl
rio|t4
from 1 to 6 days with tho majority of daatha e<wrinf la 2 to 3 d*y9
Ttaw KM modarato diarrhaa lathargy, and poor appotlto ia all of tho
mimmls
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At autopsy tho liTwr rariad fro a straw 00lor to darV r^d ia difftrant
vigours Tho intastiaaX tract was Lrritatod
' sant in tortral
inatamw*
sin^mx -
Tha LDg0 of Arcelor 12 Idlogrut Fiducial li
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BTl FEED M* TOCNGO
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