Document 1QObydbjYpXKEVkZj0oeKQKXX

22740; Federal: Register / Vol. ST,. No. 119 / Friday, June 20,. 1986. / Rules andi Regulations 2. Interlaboratory: programs Eachlaboralory analyzing asbestos, tremolite, anthophylllte, andactinolit&samples for.compliance. determinafion shan implement an interlaboratory quality assurance programthat as a minimum includes>participation of. at least two>other independent laboratories. Bach laboratory shall.partlcipate imround. robin tesUng;aUeast once.every.6,months, withiat.least alLthe other.laboratories in its . interlaboratoryquality assurance group. Each: laboratory. sKalhsubmit.slldes typical ofits own work load for use in this program. The round robin.sHair.be designed and results analyzed'usiiig appropriate statistical methodology.. 3. All.indivlduala perf6rmingyasbestos, tremolite, antHophyllite, aricfactinolite analysis must'Have taken the NIOSH course for sampling and evaluating .airborne asbestos; tremolite, anthophyllite; and. actinolite dust or an equallvalent course. 4. When the use of different microscopes contributes.to differences between counters and laboratories, the effect of the different microscope shall be evaluated and themicroscope shall be replaced; as necessary: 5. Current results of these-quality assurance programs shall!be posted-ln each laboratory to keep the-mlcroscopists Informed. Appendix.B to-1910.1001--Detailed' Procedure for.Asbestos-Tremolite,. Anthophyllite, and Actinolite Sampling and * Analysis--Non-Mandatory- Thisappendtacontatna.a.detailed ' procedure- forcsampltng and analysiaand includes those critical elements-specified-in Appendix A..Employers are-notrequired.touse this procedure, but they are.required touse Appendix'-A.The purposeof Appendix B is to provides detailed step-by-step sampling, and aimlyslsiprocedure that-conforms to the elements specified in Appendix.A..Sjnce.this, procedure may.also-standardize the .analysis and reduce variability, OSHA encourages. employers to use-thls appendix. Asbestos, Tremolite, AntfiopHyllite, and Actinolite Sampling and 'Analysis-Method Technique: Micro9copy, Phase Contrast Analyte: Fibers (manual* count)' Sample.Preparation:.Acetone/triaceUn. method Calibration;; Phase-shift detection limit, about 3-degrees. Range:100 to l300 fibers/mm.2 filter . area* Estimated limit of detection: 7 fibers/ mm.2 filter-area Sampler Filter (0.8-1.2 um mixed- cellulose ester membrane, 25-mm diameter) .Flow rate:;0:5 l/min to 2.5 l/min~(25-mm cassette} 1.0 l/min to.2.5 l/min (37- mm cassette) Sample volume: Adjust to obtain 100 to. 1300 fibers/mm.2 Shipment:.Routine- .Sample stability: Indefinite Blanlcs: 10%of samples (minimum.2) Standard-analytical,error; 0.25.. Applicability: The Working range ls 0:02 f/` cc (1920-L air sample) to:1.25 f/cc (400^L air sample). The melhod'gives anindex of airborne asbestos; tremolite.anthophyllite, andactinolite-fibers but may bo.used for other:materialb:Buch-as fibrous glass.by Inserting suitable parameters into the countinguules. The melhod'does not. differentiate between-asbestos,.tremolite,. anthophyllite,,ondactinoUte'end other fibers.. Asbestos,.tremolite. anthophyllite,.and actinolite fibers less than oa..0;25.umdiameter wiilnoi'be.detected .by this method- interferences:'Any other, airborne fiber may, interfere since all particles.meeting the counting criteria are counted Chainlike, particles may appear fibrous. High levels of nonfibrous dust particles may/obscure fibers in the field of view and raise the detection limit Reagents: l: Acetone; 2..Triacetin.(glycerol triacetate). reagent;grade Special precautions:.Acetone-is an extremely fiammablfe liquid.and precaudons must be taken not to ignite lU.Heating.of acetone must.be;done in a ventilated laboratory fume:hood using a.fiameless;. spark-free heat source. Equipment: l.Collectiomdevice: 25-mm cassette with-50-mm extension-cowliwith. ce!luldse:eflter filterv.03 to l.2 mm pore size andbackup pad. Note: Anaiyze-representative filters.for fiber background before use and discard .the filter lot if more-than 5 fibere/lOO fields are. found: 2. Personal sampling:pump<,greater than or equal to0.5 L/min. with flexible.connecting tubing. 3. Microscope*,phase contrast, with green, or blue filter. 8 to 10X eyepiece,, and 40;to 45X phase objective (total magnificaUonca400X;, numerical aperture = 0.65,to 0.75. . 4; Slides, glass, single-frost&d,.prercleaned 25 x 75 mm. 5. Cover slips, 25 x 25 mm, no. 1 Vfc unless otherwise specified by microscope, manufacturer.. 6. Knife. No: l surgical steel, curved blade* 7. Tweezers. &.F)ask,.Guth-type, in8ulated neck. 250.to 500 mL.(wilh single-holed rubber stopper, and elbow-jointed glass,tubing.,16 to. 22 cm long).. 9. Hotplate, spark-free, stirring type;, heating mantle;.or infrared lamp and magnetic stirrer.: 10. Syringe. hypodermic, with 22rgauge needle. It..Graticule. Walton-Beckett type with 100 um diameter circular. field at the specimen plane.(area = 0.00785 mm e). (TypeG-22). Note.--the graticule is custonwnade for each, microscope.. 12.,HSE/NPL.pha8e contrast test slide,. Mark It 13. Telescope, ocular phase-ring centering. 14. Stage micrometer (0.01 mm divisions). Sampling. 1. Calibrate each personal-sampling pump with a representative sampler in line. 2: Fasten the sampler to the worker's lapel as close as possible to the worker's mouth. Remove the top cover from the end of the cowl extension (open face)'and:orient face down. Wrap the joint betweenthe extender and the monitor's body with shrink tape to prevent air leaks. 3. Submil at least'two blanks (orlO% ofthe total samples, whichever is greater) for each set of samples..Remove the.caps from the field blanlccasseUes and: store the caps.and' cassettes,in a clean area (bag or box) during the sampling period: Replace the caps in'the cassettes when sampling's completed: - 4. Sample at 0.5 L/min or greater. Do not* exceed Trog-tbtal dust loading on-the filler;. Adjust sampling flow rate;.Q'(L/min); and* time'ttproduc& a. fiberdensity;.E (fibers/* mma),.of:100 to 1300 flber8/.tn (S^-xlO4 to 5x10- fibere.per 25-mm filter witheffective> collection area (A<=385 mm2)].-for;optimum counting precision<(see step.21, below). Calculate.the minimum-sampling time. Iminhnan.(min) at the action level (one-half of the current standard); L (f/cc) of the fibrous aerosol being sampled: (Ac)(E) lfn*o * (QKL)l0at 5. Remove the-field monitor at the end-of; sampling;.replace the plastlc.top cover and small'end caps;,and store.the.monitor.. 6; Ship thesamples in a rigidiconlainer with sufficient'packing-material to-prevent fostlingjor damage. Note.--Do not use polystyrene, foam in the shipping container because of electrostatic, forces.whi'ch may cause fiber loss from the sampler, filter. Sample,Preparation Note.--The object is to produce samples, with a smooth (non-grainy) background in.a medium with a refractive-index.equal to or less than 1.40. The.method below collapses . the filter.for.easier focusing.and produces . permanent mounts which are useful for quality control'and interlaboratory comparison. Other mounting techniques meeting the above criteria may also be used. e.g;, the nonpermanent field-mounting' technique used In P ft CAM 239. 7. Ensure that the glass slides and cover' slips are free of dust .andTibers. & Place 40-to 60 ml.of acetone lnto a Guthtype flask. Stopper the flask with.a.singlehole rubber stopper, through which a glass tube extends 5 to 8 cm.into the flask..The. portion of.the glass tube that exits the top.of the stopper (8to'10 cm);is bent downward in. an elbow that' makes an angle of2016 30' degrees with.the horizontal: 9: Place the flask, In a 8tirring';hotplate or wrap ln:a-heating mantle: Heat theacetone gradually to its boiling temperature-.(ca; 58 `C). Caution.--The.acetone va por must be generated in a.ventilated fume-hoodaway. from all open flames and spark sources. Alternate heating methods can be used, providing no open flame or sparks are present. 10: Mount either'the.-whole-sampfe fitter-or a wedge cut from the sample filter on a clean glass-slide. a. Cutwedges of ca. 25 percent of. the filter area with a curved-blade steel surgical knife using a.rocking motion; to prevent tearing.. GLEASON-000988