Document 1QM5dzjp76GQBaGy5zdYxXKxE

DownloadRandom document
BACK TO MAIN Study Title Growth and Reproduction Toxicity Teat with L-13492 and the Freshwater Alga, SelenastTum caprlcornutw Data RequirePsntr Guideline Uumber U.S. EPA-TSCA, Guideline 797.1050 Authors Timothy J. Hard J e A M e P. MdgaZU Robert L. Boari Study Ccopletsd AUgUpt 3, 1995 Sponsor 3H Company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, ninneaota 55144 Testing Facility T.R. Wilbury Laboratories, Inc. 40 W a k a Lane W b l e h e a d , nassachuaetts 01945 T.R. Wilbury study Number 841-TH Page 1 of 24 BACK TO MAIN I. COO0 LABoluTORI PRICLXCS coI(PLIA*cIL S T A W This study vas conducted according to EPA Cood Laboratory Practice Regulations (40 CPR 7 9 2 ) , with the following exceptions. ?'he stability of the test eubstance was assumed but not verified at T.R. wilbury Laboratories and the date of one of the 3 micropipette calibration. can not be verified from the raw data (the year was apparently entered incorrectly). Deviations fromthe study protocol are discussed in section X I 1 of this report. Study Director ' I T.R. Wilbury study N u m b e r 841-TH Page 2 of 24 -- BACK TO MAIN submitted by: T.R. Wilbury Laboratories, I`nc. 40 m a k s Lane Marblehead, Massachusetts Certification: T.R. wilbury Laboratories, Inc. study number 841-TH, Growth and Reproduction Toxkity Test vith L-13492 and the Freshvater Alga, seleriastrum capricornutum, vas audited by the Quality Assurance Unit for compliance with the protocol, standard operating procedures, and applicable Good Laboratory Practices (U.S. EPA, 1993). The original rav data and a copy of the final report will be archived at T.R. Wilbury Laboratories, Inc. for at least 10 years. Quality assurance audits were performed and the findings reported to T.R. Wilbury Latw~ratoriesmanaqement and the stcdy director on the following dates: Aspect Audited Audit Date Reported to Study Director Reported to xanagement Protocol : In-life: Draft Report: Final Report: 6 / 8/95 6/30/95 7/11/95 8 / 3/95 6/ 9/95 6/30/95 7/11/95 8 / 3/95 6/9/95 8/3/95 8/3/95 8/3/95 n Arthur P. Paradice Quality Assurance officer T.R. Wilbury study Number 841-TH Page 3 of 24 I BACK TO MAIN SECTION8 I. Good Laboratory Practice Compliance Statement 11. Quality Assurance Statement 111. Table of Contents IV. Index of Tables and Figures V. Summary VI. General Information VII. Introduction VIII. Methods and Materials i IX. Results X. References XI. signature Page XII. Protocol Deviations Appendix A. water Quality Data from Toxic!ity Test PAGE 2 3 4 5 6 7 8 8 13 19 20 21 22 T.R. Wilbury study Number 841-TH Page 4 of 24 BACK TO MAIN Table 1. Characterization of a representati-ve sample of test media and water used to formulate test media for the toxicity test vith t.he freshwater alga, Selenastrunr capricornutum, and L-13492 PAGE 9 Table 2 . Test system summary for the toxicity test with 11 L-13492 and the freshwater alga, Selenastrum Capricornut um Table 3 . Cell growth data from the toxicity test with 14 L-13492 and the freshwater alga, Sulenastrum capricornutum Table 4 . Cell growth data from the toxicity test vith 15 isopropyl alcohol and the freshwater alga, Selenastrum capricornutum Table 5. Average specific growth rate and percent change 17 1 from the control from the toxicity 'test with the ! freshwater alga, Selenastrum capricornutum, and L-13492 Table 6. Effective concentrations (EClOs, ECItOa, EC90s) 18 from the toxicity test with the freshwater alga, Selenastrum capricornutm, and L-13492 Table A.1. Temperature measured during the toxicity test 23 with L-13492 and the freshwater alga, Selenastrum capricornutum Table A.2. pH values measured during the toxicity test with 24 L-13492 and the freshwater alga, Selonastrum caprfcornut um Figure 1. Growth of selenastrum capricornutum exposed 16 to L-13492 for 9 6 hours T.R. Wilbury study Number 841-~n ,. PagB 5 of 2 4 BACK TO MAIN , .. . v. stmaax The toxicity of L-13492 t o tho freshwater alga, Selenastrumr capricornutum, is described in this final report. Tho test was conducted for 3H Company for 96 hours from June 26 to 30, 19!35, at T.R. Wilbury Laboratories, rnc. in narblehead, Xassachusetts. It was conducted by Jeanne Hagazu, Jacqueline Nevius, Peter' Kovalski, KHVen Stevens, Peter Nevius, Robert B O e r i , and Timothy Ward according to the protocol developed for T.R. wilbury study Number 841-TR. L-13492 was supplied by the sponsor. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 24 i 2.c. Nominal concentrations of L-13492 were 0 (control), 1.0, 2.0, 4.0, 8.0, and 16 mg/L. The dilution vater was sterile enriched media adjusted to a p R of 7.5. d ~ i wyith a hemocytometer. Cell counts were made 4 Exposure of algae to the test substance for 96 hours resulted in a median effective concentration (Ec50) of 4.9 mg/L L-13492, with 95% confidence limits of 3.5 and 6.7 mg/L. The 96 hour no observed effect concentration (NoEC) is 1.0 mg/L and the lowest observed effect concentration (LOEC) is 2.0 mg/L. A toxicity test vas performed simu1t:aneously with isopropyl alcohol, a component that represents 278 of L-13492. Algal growth was not affected at 4.4 mg/L (the concentration of isopropyl alcohol present in the 16 mg/L L-13492 solutions) or at 1,000 mg/L, indicating that the concentration of isopropyl alcohol in L-13492 can not, by itself, account for the toxicity of L-13492 to algae. T.R. Wilbury study Number 841-TH Page 6 of 24 BACK TO MAIN V I . 0- IWIOIUIATICJ naterial Tested: sponsor: Test Substance shipped From: Reported Purity: Study Initiation Date: E x p e r h n t a l start Date: Experimental Completion Date: Study Completion Date: Archive Location f o r the Biological Raw Data and a Copy of the Final Report: L-13492 3n Company Environmental Laboratory 935 Bush AVenUe Building 2-3E-09 st. Paul, Minnesota 55144 3n company Environmental Laboratory 935 Bush Avenue Building 2-33-09 st. Paul, flinnesota 55144 100% June 14, 1995 (protocol signed) June 26, 1995 June 30, 1955 August 3, 1995 T.R. wilbury Laboratories, Inc. narblehead, Massachusetts T.R. wilbury Study Number 841-TH Page 7 of 24 BACK TO MAIN VIX. I m o z ~ I o I This study was aponeored by 3H Company. The objective of the study was to determine the 24, 40, 72. and 96 hour effective concentrations . (EClOs, EC508, EC9Os) and no observed effect concentration (NOEC) of the test substance t o the freshwater alga, Selenastrum capricornutum, under static conditions. The test protocol was#based on U.S. EPA methoda (EPA, 1993). The report contains sections that descrih the methods and ! materials employed in the study, and rer3ults of the investigation. The report also contains an appendix that presents water quality data collected during the test. VIII. KETBODS AXD M&T&RIALS TEST SVBSTAHCE: The sample of L-13492 (T.R. Nilbury Laboratories sample Number 502) was delivered to T.R. Hilbury Laboratories on June 1 4 , 1995 in a cardboard box at ambient temperature. It was contained in a 125 ml plastic bottle. The label attached to the bottle included the following information: 'L13492, Hazardous Components/Special Precautions: 113opropyl Alcohol, Originator: S . J . nartin -. S.A. Beach, 53-QN-02 + 2-33-09, 0-5774. 8 - 7 0 6 5 , 5/4/95, 6/7/95, N2332-1'. L-13492 [a thin, clear liquid) was supplied by 3M Company, st Paul, Minnesota. Prior to use the test substance!was stored in the dark at room temperature. Test substance stock solutions were formulated in dilution water without the use of a solvent. The test substance was assumed to have a purity of loom active ingredient and to be stable under storage and testing conditions. Unused test substance will be returned to the sponsor. DILUTION HATER: Water used for acclimation of test organisms and for all toxicity testing was sterile enriched media (U.S. EPA, 1978; T.R. Hilbury Standard Operating Procedure number 6 ) adjusted to is target pH of 7.5 with 0.1 N HC1 prior to use. Characterization of a representative simple of media is presented in Table 1. TEST ORCANISHI Algae used for the test [Selenastrum c:dprIcornutm,. UTF.X 1648) was from a culture originally procured from the Culture Collection of Algae at the University of Texas at Austin and delivered to T.R. Hilbury Laboratories on April 11, 1995. The culture was transferred to sterile enriched media identical to media used for this test and maintained at test conditions for at least 14 days beforme the definitive test. The subnample of algae used to inoculate media at: the start of the definitive test came from a 7 day old culture. Identification #of the culture organisms was verified using an appropriate taxonomic key. T.R. Hilbury study Number 841-TH Page 8 of 24 BACK TO MAIN Table 1. Chemical characterization of a representative sample of test media and water used to formu1at.e test media for the toxicity test with the freshwater alga, Selenastrum caprfcornutum, and L-13492. Parameter unit of Measurement PH Total Phosphorus Nitrate Cbl oride Tocal Organic Carbon Metals Arsenic cadmium Copper Fluoride soron Aluminum chromium cobalt Iron Lead nercury Nickel silver zinc Organochlorine Pesticides plus Toxaphene Organophosphorus Pesticides PCBS pR units mg/L mg/L as N mg/L mg/L mg/L mg/L mglL mg/L mq/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L Detection 'Limit -- 0.03 0.05 1 1 0.01 0.0002 0.005 0.1 0.5 0.1 0.01 0.03 0.03 0.005 0.0003 0.03 0.02 0.02 0.5 2 0.52 0.5 Measured Value 1.5 0.46 0.08 14 ND ND 0.0002 WD ND ND ND ND ND 0.08 ND ND ND ND ND ND ND ND ND Notes: 1. The pH was measured in a control test vessel at the start of the test. Total organic carbon, total phospho.cus, nitrate. chloride, fluoride, metals, pesticide, and PCB data were collected during February, 1995, as part of routine biannual water quality testing (these analysis were perEormed by Pace, Inc., Hampton, New Rampshire). 2. ND = not detected at or above the detection limit. T.R- uilbury study Number 841-TH Page 9 of 24 BACK TO MAIN .. .. . TOIICITI R S T : Results of a range finding test were used to select the concentrations for the definitive teat. The range finding test was - performed from June 15 to 19, 1995. d4t the conclusion of the test the number of cells/ml equalled the following percent of the control: 0.10, and 1.0 mg/L = >89m, 10 mg/L = 4m, 100 mg/L = 2%, and 1,000 mg/L <im. The definitive toxicity teat was performed from June 26 to 3 0 , 1995, according to T.R. Wilbury Protocol 841-TH (crovth and Reproduction Toxicity Test with L-13492 and the Freshwater Alga, Selenastrum caprfcornutum). The protocol, which Vats signed by the study director on June 14, 1995, is based on procedures of the U.S. Environmental Protection Agency (1993). A summary of the test system is presented in Table 2. The test was conducted at a target.temperature of 24 f 2.C with five *-sncentrationsof test substance and a dilution wator control. Nominal concentrations of the test substance were: 0 mg/L (control), 1.0, 2 . 0 , 4.0, 8.0, and 16 mg/L. A 16 mg/L stock solution vir) formulated o n June 26, 1995 by adding 0.016 g L-13492 to sterile enriched media and adjusting the final media volume to 1,000 ml. Appropriate amounts of this stock solution were added directly to dilution vater to formulate test media. A test was performed simultaneously vith isopropyl alcohol (Wallinckrodt, Lot 3 0 3 S ) , a component that represents 279 of L-13492. This test was conducted at 4.4 mg/L (the concentratio:n of isopropyl. alcohol in vessels containing 16 mg/L L-13492) and at 1.000 mg/L. A 1,000 mg/L stock solution was formulated on June 26. 1995 by adding 0.S g isopropyl alcohol t o sterile enriched media and adjusting the final redia volume to 500 ml. Algae were distributed among three replicates of each treatment at the rate of 10.000 cells/ml. The test vas perfoxed in 250 ml glass Erlenmeyer flasks that contained 100 ml of test solution. Test vessels were randonly arranged on a rotary shaker adjusted to 100 rpm in an incubator during the test (a random numbers table vas used to select the location of each vessel). A 24 hour light and 0 hour diark photoperiod vas automatically maintained with cool-vhite fluorescent lights that provided a light intensity of approximately 35O-XO footcandles. The number of algal cells/ml in each test vessel and the Occurrence of relative size differences, unusual cell shapes, colors, flocculations, adherence of cells to test containers, or aggreptic;n af calls vas determined visually by means of direct microscopic examination vith a hemocytometer. Cell counts were made and recorded. daily during the 96 hour test. The determination of whether toxic effects were algistatic o r algicidal was performed at the concluriion of the toxicity test (0.5 ml of media from each 16 mg/L vessel was transferred to a vessel containing fresh media without L-13492 and incubated for 192 hours under test conditions). T.R. Wilbury study Number BII-TH Page 10 of 24 --- BACK TO MAIN Tablo 2. Test system summary f o r tho tonicity test with L-13492 m d tho freshwater alga, Selena#trum c:aprfmmutura. . .. species I Culture Acclimation Period: %lenastrum Capricornutuai >14 days Test Duration: 96 hours Test Vessels: 250 ml glass flasks V o h m of Test Media: 100 m1 Number of Replicate Test Vessels: 3 Xnoculun: 10,000 cells per m l Oscil1ation Rate: 100 r p m Incubator Temperature Range: 23.4-23.7.C Photoperiod: 24 hours light and 0 hours dark. Light Intensity: -400 footcandles water Qual ty Ueasurements: pB in each test vessel at the beginning and end of the test. Incubatoi: temperature daily. Dilution Water analysis is summarized in Table 1. End Points 24, 48, 1 2 , and 96 hour EC10, EC50, and EC90; no observed effect concentration. I Acceptability Criteria: Logarithmic growth at !)6 hours and acceptable temperature range. T.R. Nilbury study Number 841-TH Page 11 of 24 BACK TO MAIN ... . ,. . . Temperature of the incubator was measured and recorded daily (thenmmater number 2968), and pu (Beckman eodel&BI 12 nwter; instrument number 124) was determined in each test vessail at the beginning and end of the test. The temperature in a representative vessel of water incubated with the test vessels was continuously reco:ded. STATISTICAL JaCTBODSt The average specific growth rate was calculated as the natural log of the number of celle/ml at 24, 48, 72, and 96 hours minus the natural log of the number of cells/ml at 0 hours divided by the exposure period. The percent change from the control was calculated by subtracting the treatment average specific growth rate from l:he control average specific growth rate, dividing the difference by the average specific growth rate in the control, and multiplying that value by 100. Results of the toxicity test were interpreted by standard statistical techniques. The statistical method used for calculating 24, 48, 72, and 96 hour EC values (and confidence intervals) was based on a nonlinear regression estimation procedure (Bruce and verstetig, 1992). All calculations were performed using the averago specific grtwth rates and nominal concentrations of L-13492. The no observed effect concentration (NOEC) was deterslined using a parametric one-way analysis of variance (ANOVA) and average specific grovth rate in each test vessel at the end of the test. T.R. Wilbury Study N u m b e r 841-TH .. Page 12 of 24 BACK TO MAIN No insoluble material was noted in any test vea'sel during the test. The algal population in the control test vessels grew well, resulting in an average of 1 , 2 4 9 , 0 0 0 cells/ml after !a6 hours (Table 3 ) . Water quality throughout the test vas within acceptab.le limits (Appendix A). The range of incubator temperatures was 2 3 . 4 to 23.7.C (Table A.1) and the pa of test media was not affected by the test substance at the beginning of the test (Table A.2). Biological data generated by the acute toxicity test vith L-13492 are presented in Tables 3 and 4. The grovth curve for algae exposed to the test substance for 96 hours is presented in Figure 1 (the slope of the dose-response curve is 3 . 3 ) . The 48, 72, and 96 hour I?C10, ECSO, and EC90 values for algae exposed to L-13492 are presented in Tasbble 6. The 96 hour ECSO (and associated 95 percent confidence limits) is 4 . 9 mg/L ( 3 . 5 and 6 . 7 mg/L). NO effects (size differences, unusual cell shapes, colors, flocculations, adherence of cells to test containers, or aggregation of cells) were noted. The 96 hour no observed effect concentration (NoEc) is 1.0 mg/L and the lovest observed effect concentration (LOEC) is 2 . 0 mg/L L-13492. The determination of vhether toxic effects were algistatic or algicidal was performed at the conclusion of the toxicity test. A 0.5 m l aliquot of media from each 16 mg/L vessel vas transferred to a vessel containing fresh media vithout L-13492 rrtd incubated for 192 hours under test conditions. Algae increased from 400 cells/mL to 580,000 cells/mt, indicating that the effect of L-13492 at this concentrat.ion was algistatic rather than algicidal. Biological data generated by the exposure of algae to isopropyl alcohol are presented in Table 4 . Algal growth vas not affected at 4.4 mg/L or 1,000 mg/L. indicating that the concentration of isopropyl alcohol in L-13492 can not, by itself, account for the tIDxicity of L-13492 to algae. T.R. Wilbury study Number 841-TH Page 13 of 24 BACK TO MAIN ' T b b h 3. Cell qrovth data from the acute toxicity teat with L-13492 and tho Ireahwater alga, selena8tnun caprfcornuturn. Noainal Concentration Of 1-13492 - rep Number of Cells/ml x IO' (hour) 0 2a 48 72 96 0 (control) 1.0 2.0 4.0 8.0 16 1 2 3 mean 8 control 1 2 3 mean 8 control 1 2 3 mean o control 1 2 3 mean a control 1 2 3 mean % control 1 2 3 mean % control 10 2 2 10 20 10 23 -1-0 2-.:!. lo 2 0 10 1 El 10 2 El 10 22 100 100 10 11 10 18 10 19 10 16 100 73 10 26 10 19 10 14 10 20 100 91 10 18 10 16 10 21 10 18 100 82 10 12 10 11 10 18 10 14 100 64 134 538 1,132 132 568 1.260 114 614 1,356 1-2-7 5-7-3 -- 1,249 142 606 1,378 112 592 1,156 118 610 1.400 124 603 1,311 98 105 105 140 178 270 102 198 252 136 202 304 126 193 275 99 34 22 86 100 106 78 160 102 80 104 132 81 121 113 64 21 9 44 66 98 52 72 80 40 48 74 45 62 84 35 11 7 34 40 24 38 70 34 34 38 22 35 49 27 28 9 2 T.R. wilbury study Numbttr 841-TH Page 14 of 24 .- BACK TO MAIN . . . I ' Tab10 4. coll growth d.ta from tho acute toxicity temt vith isopropyl alcohol and tho fromhwater alga, Sdenaatrum caprfcornutua. Nominal Concantration of L-13492 (WL) rep. ~ Nluabar of Cells/ml x 10' (hour) 0 24 48 72 96 0 (control) 4.4 1,000 1 2 3 mean N control 1 2 3 mean N control 1 2 3 mean 8 control 10 22 10 20 10 23 -1-0 -2-2 10 14 10 32 10 16 10 21 100 95 10 31 10 26 10 24 10 .2 7 100 i.23 134 538 1,132 132 568 1,260 114 614 1,356 1-2-7 5-7-3 1,24-9- 112 584 1.236 128 554 1,270 144 584 1,064 128 574 1,190 101 100 95 136 608 1,370 144 580 1,338 106 512 1.334 129 567 1,347 102 99 108 T.R. Wilbury study Number 841-TH Page 15 of 24 BACK TO MAIN P c 0 L 5 0 n 100000 z loo00 Figure 1. Growth of selenastrum caprfcornutum exposed to L-13492 for 9 6 hours. T.R. wilbury study Number 841-TH Page 16 of 24 BACK TO MAIN Table 5. Average specific growth rate and percent change from the control from the toxicity teait with the freshwater alga, Selenastrua capricornutum, and L-13492. Nominal Concentration Of L-134 92 (mg/L) Average Specific Growth Rate 24hr 48hr 72hr 96hr 0 (control) 0.032 0.053 0.056 0.050 1.0 0.032 0.052 0.057 0.051 2.0 0.018 0.053 0.041 0.035 4.0 0.027 0.044 0.035 0.025 8.0 0.025 0.031 0.025 0.022 16 0.012 0.026 0.022 0.010 Percent Change Prom control 24hr 48hr 72hr 96hr -- *-- -_ __ 0 2 -2 0 44 0 27 30 16 17 37 5 0 22 4.2 55 56 63 5:L 61 80 T.R. Wilbury study Number 841-TH Page 17 of 24 BACK TO MAIN Tabla 6. Effective concentrations (EClOs, ECSOs, LC90s) from the toxicity t e s t with the freshwater alga, S e l o ~ s t r u n c, qrfcorautm, and L-13492. 24 hours 48 hours 72 hours 96 h o u r s EC Value (ML) -- EClO ql.0 EC5O 15 --- EC90 = >16 EClO <1.0 EC50 14 EC90 >16 -EClO = <1.0 EC50 7.1 EC90 = >16 EClO EC50 EC90 - = = <l.O 4.9 >16 9!i Percent Confidence Limits m/L) t1.0 - 9l.8 >16 - 7.7 >16 -<l.O 8.2 >16 >16 < 4.7 l . - O 11 >16 -t1.0 3.5 6.1 >16 T.R. Wilbury Study Number 841-TH Page 18 of 24 BACK TO MAIN BlUCe, R.D., and Versteeg. 1992. A statistical Procaduse for nodeling Continuous Toxicity Data. Environ. Toxicol. and C b m - Vol.11. No. 10, pp. 1,485-1,494. U . 5 . EPA. 1978. The selenastrum caprfcornutum Printz Algal Assay Bottle Test. EPA-600/9-?B-018. Environmental Research Laboratory, Corvallis, Oregon. U.S. EPA. 1993. 40 CFR Part 797. Toxic substances control Act Test Guidelines; Final Rules. Section 797.1050. U.S. EPA. 1993. 40 CFR Part 792. Toxic Substances C0nt:rOl Act (TSCA); Good Laboratory Practice Standards; Final Rule. T.R. wilbury study N u m b e r 841-TH page 19 of 24 BACK TO MAIN XI. SIOYITUR. PAGE Jeanne P. nagdzu ! study Director Aquatic TOXiCOlOgiSt Robert L. Boeri Coauthor 'I < ; 1, r ; , -l.'<. Peter d . Rciralski Aquatic Toxicologist Q 7 '*5 for: Jacqueline'M.'Mevius Aquatic Toxicologist Keven X. s:evens Biologist 6 I I. - . .p Peter C. Nevius Biologist 6 J3 /!'is Author T.R. Wilbury Study Number 841-TH Page 20 of 24 BACK TO MAIN The protocol vas amended to specify the nominal concentrations for the definitive test and to note that a test ~ u l adlso be conducted with 4.4 and 1,000 mg/L isopropyl alcohol. PROTOCOL DeVI&TIOWS: NO deviations were made from the protocol. T.R. Wilbury study Number 841-TH BBACK TO MAIN BACK TO MAIN Table A.l. Temperature meaaured d u r i n g t h e t o x i c i t y t e s t v i t h L-I3492 and the freshwater alga, Selenastrum caprlcornultum. Hour o f Exposure Temperature of Incubator (.e) 0 23.6 24 23.6 48 23.4 72 23.4 96 23.7 T.R. Wilbury s t u d y Number 841-TH Page 23 of 24 BACK TO MAIN . .. . Table A.2. pH value. measured during the toxicit'j test with L-13492 and the freshwater alga, selsnastrrto capricornutlm. . .. Nominal Concentration (WL) T0.t with L-13492 0 (control) Replicate 1 1.0 2.0 4.0 8.0 1 2 3 16 1 2 3 T e s t with Isopropyl Alcohol 4.4 1,000 pa Initial Final 1.5 7.5 1.5 1.5 1.f 1.5 7.5 1.5 7 -5 7.4 7.4 _ - 7.4 7.4 7.4 1.4 1.4 7.4 7.4 9.8 10.4 10.0 9.8 9.9 9.8 8.6 8.3 8.2 8.0 8.0 7.9 7.7 1-1 1.1 7.6 1.6 1.6 7.4 7.4 .-10.0 *".I 7.4 10.5 7.4 10.4 1.4 9.9 7.4 9.9 T.R. wilbury Study Number 841-TH Page 24 of 24 i i -1 u4/ U17-2uUl t m t N I AL L M U H A I U MY I ARCHIVED FINAL REPORT LAB REQUEST NO. N2332 REQUESTOR NAME: SAB/S.J.MARTIN DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 06/07/1995 DESC: L-13492 ECOTOXICITY EVALUATION CONTRACT LAB(S): TRWIL EXP COMP DATE: 08/30/1995 DATE COMPLETED: 09/02/1995 PROJECT LEAD: PHONE NO: 3M FAX NO: 651-778-6176 Tests conducted f o l l o w i n g 40CFR 797; methods 1050, 1300, 1400. Sample desc.: L-13492, 45% s o l i d s , from S. Martin. SAB 6/7/95 BACK TO MAIN Sample i s a c l e a r l i q u i d . L o t confirmed as L o t #2 as per S.J. Martin, o f 3M SCD Lab on 7/18/95. SAB Results from 48-hr Daphnia magna acute t o x i c i t y t e s t : Test Conc. , mg/L 0 (control) 13 22 36 60 100 Percent Immobilization 24-hrs Exposure 0 0 5 0 0 $-4' 70 and/or Mortality 48-hrs Exposure 0 0 5 55 100 100 sab 7/31/95 Results from 96-hr Fathead minnow acute s t a t i c t o x i c i t y t e s t : Test Conc. mg/L 0 (control) 130 220 360 600 1000 24-hrs exp 0 0 0 0 0 50 Percent Mortality 48-hrs exp 72-hrs exp 0 0 0 Q 0 0 0 0 0 0 60 7Q sab 7/31/95 Results from 96-hr algae growth i n h i b i t i o n t e s t : Test conc. mg/L 1. o 2.0 4.0 8.0 16 EC50 95% C . I . sab 9/1/95 24-hrs 0 44 16 22 63 15 (9.8 - exp. >16) Percent change from 48-hrs exp. 2 17 17 42 51 14 (8.2 - >16) Control 72-hrs exp. -2 27 38 55 61 7.1 (4.7 - 11) 96-hrs exp 0 0 0 0 0 70 96-hrs exp. 0 30 50 56 80 -4.9 (3.5 6.7) * = CONTRACT LAB DATA 04/01 /2001 ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT LAB REQUEST NO. N2332 CONTINUED ---------------------------------------------------------------,----------------- SAMPLE DATE CODE DESCRIPTION RESULT ---------------------------------------------------------------,----------------- 1 0 5 / 0 4 / 9 5 L-13492 Lot 2 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST - TRWIL 96-HR-LC50 *FISH ACUTE TEST 96 HOUR - TRWIL IC50-ALGAE *ALGAE ASSAY I C 5 0 * = CONTRACT LAB DATA 34 MG/L 890 MG/L 4.9 MG/L BACK TO MAIN 2 MDL o r (95% C.I.) - ( 3 0 - 39) (600 - >1000) (3.5 6.7)