Document 15w3NNVE6BX2B4vgr3QBORpNj

* UNITE. D STA TES AGENCY ENVIRONMENTA PROTECT REGION 1 NEW ENGLAND REGIONAL LABORATORY NORTH CHELMSFORD, MA 01863 MEMORANDUM July 1st, 2024 Subj:Concentrated Animal Feeding Operation (CAFO) Sampling at Gosliga Farm From:Nafisah Ali, Inspector Digitally signed by Ali, Laboratory Services and Applied Science Division (LSASD)Ali, Nafisah Date: 2024.07.22 Field Services Branch (FSB2)Nafisah 12:11:12 -04'00 ' Reviewed By: Eric Magnan, ManagerERIC Digitally signed by ERIC MAGNAN LSASD - FSB2MAGNAN Date: 2024.0713: .03: 230-042'00 ' To:Andrew Spejewski, Inspector Enforcement and Compliance Assurance Division (ECAD) Water Compliance Section 1 I. Facility Information A. Facility Name: Gosliga Farm B. Facility Location: 319 Sunset Lane Vergennes, VT 05491 C. Facility Contact: N / A D. NPDES Permit ID: N / A II. Background Information A. Date / Start time of sampling inspection: 5/22/2024 09:50 B. Weather conditions: Sunny, 82 F C. USEPA Representatives: Andrew Spejewski, Eleanor Horvath, Nafisah Ali, Anna Hatke D. State / Local Representatives: Nate Sands, VT DEC E. Federally Enforceable Requirements Investigated: N / A III. Disclaimer: Unless otherwise noted, this report describes conditions at the facility / property as observed by EPA inspector(s), and / or through records provided to and / or information reported to EPA inspector(s) by facility representatives and as understood by the inspector(s). This report may not capture all operations or activities ongoing at the time of the inspection. This report does not make final determinations on potential areas of concern. Nothing in this report affects EPA's authorities under federal statutes and regulations to pursue further investigation or action. ED_019088A_00004944-00001 EPA CAFO Compliance Sampling Inspection IV. Type and Purpose of Sampling Inspection(s) The purpose of the compliance sampling inspection was to assess applicability of and compliance with the CAFO requirements under the Clean Water Act at 40 C.F.R. Part 122.23. V. Sampling Inspection Summary On Wednesday, May 22nd, 2024, Nafisah Ali, and Anna Hatke of FSB 2 (" the EPA Sampling Team "), conducted wastewater sampling of five locations at Gosliga Farm (see photos and description included as Attachment 1). The sampling activities were conducted in accordance with the Standard Operating Procedure for the Collection of Chemical and Biological NPDES Water and Wastewater Samples FSBSOP- NPDES Water Revision 2, 2020. The EPA sampling team collected one outfall grab sample (GF01). Two in - stream samples (GF02 and GF03), one grab sample from manure pit (GF04) and one grab sample from leachate pit (GF05) for analysis of E. coli (Colilert - MPN), Total Suspended Solids (TSS), Source Tracking Analysis ($ STA), Ammonia (AMMO), Total Nitogen (TN) and Total phophorous (TP). The E. coli samples were collected in 120mL UV Sterile, pre - preserved bottle with Sodium Thiosulfate. Total Suspended Solids samples were collected in 1-liter (L) High Density Polyethylene (HDPE) bottle. Source Tracking Analysis samples were collected in 1- L Amber glass teflon, lined cap bottle. Ammonia, Total Nitogen and Total Phophorous were collected in one 500 mL HDPE bottle with preservative Sulfuric Acid to attain a pH of < 2. There were no split samples with the facility. The samples collected by the EPA sampling team were then placed in coolers with ice to maintain an internal temperature of less than 4 degrees Celsius. Samples will be analyzed for E. Coli, at Endyne Laboratory, 56 Etna Road Lebanon, NH 03766. Samples for TSS, $ STA, TN, and TP will be analyzed at the EPA New England Regional Laboratory in North Chelmsford, MA. VI. Description of Sampling Location (in order of collection time) Photographs depicting each sample location are included in Attachment 1 of this report. GF01: One circular PVC outfall located at Gosliga Farm, approximately 1.5 feet in diameter. Discharge flow was approximately < 1 gallon per minute into unnamed stream / brook discharging into Lake Champlain. Sample location was located at the base of hill and Southwest of dairy barns and the feed bunker lot. Grab sample was collected at the mouth of the outfall as indicated by red X. A sweet, fermented odor was detected. Color was clear - yellow, and turbidity was low. White froth was observed exiting the outfall. The matrix of the stream bed was observed to be muddy and loose, filled with organic vegetation and detritus. Some invertebrates and nematodes were observed directly downstream of the outfall. GF02: In - stream sample of an unnamed brook discharging into Lake Champlain collected mid- field at Gosliga Farm. The sample was collected approximately half a foot from the Northern bank and approximately 2 feet downstream of a metal culvert pipe 2 feet in diameter with minimal - to - no flow as indicated by red X. No notable odor was detected. Color was gray - brown and opaque, and turbidity was high. Tall grasses were observed growing around the waterway which created a buffer of at least 6 feet between the sample location and mowed fields of the farm. Sampling Inspection Date: 5/22/2024 ED_019088A_00004944-00002 EPA CAFO Compliance Sampling Inspection GF03: In - stream sample of an unnamed brook discharging to Dead Creek collected at Gosliga Farm. The sample was collected approximately half a foot from the Northern bank and approximately 600 feet upstream of GF02 as indicated by red X. No notable odor was detected. Color was murky and yellow - brown, and turbidity was medium - high. Some organic matter was observed floating in - stream. Tall grasses were observed growing in and around the stream. Some invertebrates were observed in - stream. GF04: A grab sample from a large remote stagnant manure pit was collected at Gosliga Farm. The sample was collected approximately 3 feet from the southwestern bank indicate by the red X. A strong smell of manure was observed. Color was muddy, brown, and opaque. Turbidity was high and a high concentration of organic fecal floatables was observed. Tall grasses were observed growing around all sides of the square pit, except for the concrete access point. A headway of approximately 1.5 feet was observed between the bank and the manure pit level. GF05: A grab sample from a large stagnant leachate pit was Collected at Gosliga Farm. The leachate pit was located downhill of the dairy barns and approximately 300 feet from the feed bunker lot. The sample was collected approximately 3 feet from the southern bank as indicated by the red X. A musty and sweet smell was observed. Color was reddish brown and opaque and turbidity was high. No floatables were observed. Tall grasses were observed growing around all sides of the square pit, except for the concrete access point. A headway of approximately 1 foot was observed between the bank and the leachate water level. VII. EPA Sampling and Analytical Results The table below provides a summary of the laboratory analytical results collected during the inspection. For a full list of compounds analyzed for each parameter and their results, refer to the laboratory reports that are attached to this inspection report (Attachment 2). Sampling Inspection Date: 5/22/2024 ED_019088A_00004944-00003 EPA CAFO Compliance Sampling Inspection Table 1: Summary of Gosliga Farm (GF01, GF02, GF03, GF04, GF05) May 22nd, 2024, analytical results. Location Site ID GF01 GF02 GF03 GF04 GF05 Bacteria Sample date 5/22/24 5/22/24 5/22/24 5/22/24 5/22/24 Sample time 09:50 10:13 10:33 10:54 11:17 (MPN / 100mL) E. coli > 2400 > 2400 2400 17000 8200 Ammonia AMMO NA s, NA s, NA (mg / L) Total Nitrogen TN 3400 830 1500 ANR ANR (ug / L) Total Phosphorous TP 770 1600 1600 160000 12000 (ug / L) TSS (mg / L) TSS 7.0 80 68 8200 290 PPCPs (ng / L) Cotinine 1.0 2.2 2.2 s, NA Acetaminophen 16 5.7 12 s, s, Paraxanthine 19 4.7 6.0 s, NA Atenolol ND ND ND s, s, Caffeine 15 7.8 9.1 s, s, Metoprolol ND ND ND s, s, Diphenhydramine ND ND ND s, s, Carbamazepine ND ND ND NA NA * NA = Not Analyzed * ANR = Analysis not required * ND = Not Detected Attachments: Attachment 1: Photo of sampling locations (GF01, GF02, GF03, GF04, GF05) and descriptions at Gosliga Farm. Attachment 2: Laboratory Reports for Analysis (E. coli, TSS, $ STA, TN, TP) Note: Pages of Laboratory Reports have been redacted, the information that has been redacted does not pertain to this facility. Sampling Inspection Date: 5/22/2024 ED_019088A_00004944-00004 EPA CAFO Compliance Sampling Inspection Attachment 1: Arial image of GF01, GF02, GF03, GF04, GF05 at Gosliga Farm. GF04 GF02 GFOS GPOI Photo of sampling location VF01 at Veldman Farm. Sampling Inspection Date: 5/22/2024 ED_019088A_00004944-00005 EPA CAFO Compliance Sampling Inspection Photo of sampling location VF02 at Veldman Farm. Photo of sampling location VF03 at Veldman Farm. Sampling Inspection Date: 5/22/2024 ED_019088A_00004944-00006 EPA CAFO Compliance Sampling Inspection Photo of sampling location VF04 at Veldman Farm. Photo of sampling location VF04 at Veldman Farm. Sampling Inspection Date: 5/22/2024 ED_019088A_00004944-00007 EPA CAFO Compliance Sampling Inspection Attachment 2: Laboratory Reports for Analysis (E. coli, TSS, $ STA, TN, TP) Note: Pages of Laboratory Reports have been redacted, the information that has been redacted does not pertain to this facility. Redacted Information E. coli: Total of 4 pages TSS: Total of 7 pages TN: Total of 18 pages, pages 1-8, 13-18 pertains to this facility. $ STA: Total of 15 pages, pages 1-5, 13-15 pertains to this facility. TP: Total of 8 pages Sampling Inspection Date: 5/22/2024 ED_019088A_00004944-00008 ENDYNE Inc. Environmental Laboratories Page 1 of 2 Laboratory Report EPA 089621 11 Technology Drive Chelmsford, MA 01863. PROJECT: E. coli Analysis WORK ORDER: 2405-14444 DATE RECEIVED: May 22, 2024 DATE REPORTED: May 28, 2024 SAMPLER:Nafisah Ali Enclosed please find the results of the analyses performed for the samples referenced on the attached chain of custody located at the end of this report. The column labeled Lab / Tech in the accompanying report denotes the laboratory facility where the testing was performed and the technician who conducted the assay. A " W " designates the Williston, VT lab under NELAC certification ELAP 11263; " R " designates the Lebanon, NH facility under certification NH 2037 and " N " the Plattsburgh, NY lab under certification ELAP 11892. " Sub " indicates the testing was performed by a subcontracted laboratory. The accreditation status of the subcontracted lab is referenced in the corresponding NELAC and Qual fields. This NELAC column also denotes the accreditation status of each laboratory for each reported parameter. " A " indicates the referenced laboratory is NELAC accredited for the parameter reported. " N " indicates the laboratory is not accredited. " U " indicates that NELAC does not offer accreditation for that parameter in that specific matrix. Test results denoted with an " A " meet all National Environmental Laboratory Accreditation Program requirements except where denoted by pertinent data qualifiers. Endyne, Inc. warrants, to the best of its knowledge and belief, the accuracy of the analytical test results contained in this report, but makes no other warranty, expressed or implied, especially no warranties of merchantability or fitness for a particular purpose. Reviewed by: Alley Hart Alexander J Rakotz Laboratory Director Lebanon, NH www.endynelabs.com NELA ACCREDITEDCCREDI REDITRO 160 James Brown Dr., Williston, VT 0549556 Etna Road, Lebanon, NH 03766 Ph 802-879-4333Fax 802-879-7103Ph 603-678-4891 Fax 603-678-4893 RABORATOR FABORATORY ELAP 11263NH2037 ED_019088A_00004944-00009 Page 2 of 2 Laboratory ReportDATE REPORTED: 05/28/2024 CLIENT: EPAWORK ORDER: 2405-14444 PROJECT: E. coli AnalysisDATE RECEIVED:05/22/2024 004Site: GF 01Date Sampled: 5/22/24 Time: 9:50 ParameterResult Units Method Analysis Date / Time Lab / Tech NELAC Qual E. coli> 2400MPN / 100 mL SM20 9223B QuantiTray5/22/2415:54 RAJRA 005Site: GF 02Date Sampled: 5/22/24 Time: 10:13 ParameterResult Units Method Analysis Date / Time Lab / Tech NELAC Qualwww.. E. coli> 2400MPN / 100 mL SM20 9223B QuantiTray5/22/2415:54 RAJRA 006Site: GF 03Date Sampled: 5/22/24 Time: 10:33 ParameterResult Units Method Analysis Date / Time Lab / Tech NELAC Qual E. coli2400MPN / 100 mL SM20 9223B QuantiTray5/22/2415:54 RAJRA 007Site: GF 04Date Sampled: 5/22/24 Time: 10:54 ParameterResult Units Method Analysis Date / Time Lab / Tech NELAC Qual E. coli17000MPN / 100 mL SM20 9223B QuantiTray5/22/2415:54 RAJRA 008Site: GF 05Date Sampled: 5/22/24 Time: 11:17 ParameterResult Units Method Analysis Date / Time Lab / Tech NELAC Qual E. coli8200MPN / 100 mL SM20 9223B QuantiTray5/22/2415:54 RAJRA ENDYNE Inc. www.endynelabs.com ED_019088A_00004944-00010 E. coli AnalysisEndyne Inc. COCIah Ilse WO # Prepared: 1/31/23 2405-14444 Bill To:Report To: EPA Nafisah AliCust # 2405-14444 EPA 11 Technology Drive, Chelmsford 01863E. coli Analysis Email: ali, nafisah@epa.gov LOCATION GF01Sampled Date / Time:5/22/24 @ 9:50 AM Sampler:NA E. coli1-120ml Sterile Plastic< 10C, Na2S203 LOCATISO ampleN d Dat e / TO ime:F 5/22/ 24 @ 0 10:132 Am S ampleN r: A E. coli1-120ml Sterile Plastic< 10C, Na2S203 LOCATION OF 03Sampled Date / Time:5 / 22 / 24 @ 10: 33Am Sampler:NA E. coli1-120ml Sterile Plastic< 10C, Na2S203 $ 25.00 Per Sample * Samples MUST be received within 6 hours of sample collection **** ' Samples must be filled to the bottom of the bottle's cap where the lid screws on. Relinquished by:Accepted by: Date TimeDate Time Relinquished by:Received by: Sites / Parameters correct as listed. Client initials Date TimeDate Time Client Authorization to use Subcontract lab Client Initials Delv:Tmpl CkLab use Only Temp C:Log by Sample origin: VT NH NY OtherComment: Special reporting instructions:(PO #) Requested Turnaround Time: Routine: Rush Due Date 160 James Brown Dr.56 Etna Road315 New York Rd. ENDYNE Inc. Williston, VT 05495Lebanon N 03766Plattsburgh, NY 12903 Ph 802-879-4333Ph 603-678-4891Ph 518-563-1720 www.endynelabs.comFax 802-879-7103Fax 603-678-4893Fax 518-563-0052 Loft ED_019088A_00004944-00011 E. coli AnalysisEndyne Inc. COCLab Use WO # Bill To:Report To: Prepared: 1/31/23 Cust #089621 GEN Ph:Email: ECOL! Page 1 of 1 LOCATISO ampleN d Dat e / G Time:F 5/22 /24 @0 10: 4 54Am SamplN er: A E. coli1-120ml Sterile Plastic< 10C, Na2S2O3 LOCATISaO mpled N Date / TimG e:5/r 22/24o @ 11:s 17Am SampN ler: A E. coli1-120ml Sterile Plastic< 10C, Na2S2O3 LOCATIONSampled Date / Time:@Sampler: E. coli1-120ml Sterile Plastic< 10C, Na2S203 LOCATIONSampled Date / Time:@Sampler: E. coli1-120ml Sterile Plastic< 10C, Na2S2O3 LOCATIONSampled Date / Time:Sampler: E. coli1-120ml Sterile Plastic< 10C, Na2S203 LOCATIONSampled Date / Time:Sampler: E. coli1-120ml Sterile Plastic< 10C, Na2S203 $ 25.00 Per Sample ** Samples MUST be received within 6 hours of sample collection *** Samples must be filled to the bottom of the bottle's cap where the lid screws on. Relinquished by:Accepted by: Date TimeDate Time Relinquished by:Received by:55/77/24 1530 Sites / Parameters correct as listed. Client Initials Date TimeDate Time Client Authorization to use Subcontract lab Client Initials Delv:Tmpl CkLab use _ O_ nly Temp C:6.ScaLog by Sample origin:VTNHNYOtherComment: Special reporting instructions:(PO #) Requested Turnaround Time: Routine: Rush Due Date 160 James Brown Dr.56 Eina Road315 New York Rd. Williston, VT 05495Lebanon, NH 03766Plattsburgh, NY 12903 ENDYNE Inc.Ph 802-879-4333Ph 603-678-4891Ph 518-563-1720 Fax 802-879-7103Fax 603-678-4893Fax 518-563-0052 www.endypelabs.com2of2 ED_019088A_00004944-00012 United States Environmental Protection Agency EPALaboratory Services and Applied Science DivisionPage 1 of 7 Region 1, New England11 Technology Drive North Chelmsford, MA 01863-2431 June 10, 2024 Laboratory Results Nafisah Ali US EPA Region 1 Project No:24050033 Project:Concentrated Animal Feeding Operation Sampling Inspectio Analysis:Total Suspended Solids in Water EPA Chemist:Holly Westbrook Date Samples Received by the Laboratory: 05/23/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log - in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP - TSS - TDS7. The SOP is based on SM 2540 D. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. If you have any questions please call me at 617-918-8340. Sincerely, DANIEL Digitally signed by DANIEL BOUDREAU BOUDREAU D1a3t:e21:: 2220 2-404.'0060. 1' 0 24050033TSS ED_019088A_00004944-00013 Qualifiers: Page 2 of 7 RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value J1 = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E-Estimated value exceeds the calibration range L = Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P = The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC / MS. A = Suspected Aldol condensation product. N Tentatively identified compound. ANR Analysis not required. 24050033TSS ED_019088A_00004944-00014 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 3 of 7 Concentrated Animal Feeding Operation Sampling Inspection Total Suspended Solids in Water Matrix: Wastewater Sample Number Lab ID Collected Analysis Concentration RL mg / L mg / L Qualifier GF01 AC13039 05/22/2024 9:50 05/28/2024 7.0 2.5 Comments: GF02 AC13040 05/22/2024 10:13 05/28/2024 80 5.0 Comments: GF03 AC13041 05/22/2024 10:33 05/28/2024 68 4.0 Comments: GF04 AC13042 05/22/2024 10:54 05/28/2024 8200 500 Comments: Only 2 mLs could be filtered, reporting limit raised accordingly. GF05 AC13043 05/22/2024 11:17 05/28/2024 290 67 Comments: Only 15 mLs could be filtered, reporting limit raised accordingly. Blank Comments: 05/28/2024 ND 2.5 24050033TSS ED_019088A_00004944-00015 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 4 of 7 Concentrated Animal Feeding Operation Sampling Inspection 24050033TSS ED_019088A_00004944-00016 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 5 of 7 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Duplicate Results SAMPLE SAMPLE DUP PRECISION QC RESULT RESULT RPD LIMITS SAMPLE ID PARAMETER mg / L mg / L% (% RPD) AC13039 Total Suspended Solids in Water 7.0 6.5 7.4 25 24050033TSS ED_019088A_00004944-00017 Page 6 of 7 US EPA Region 1CHAIN OF CUSTODY RECORDPage: of 11 Technology Drive, N. Chelmsford, MA 01863 PROTECTION EPA Project No:Project Name & Location:[Sampler's Name and Signature: 24050033. Concentrated Animal Feeding Operation (CAFO) Sampling Inspection Nafisah Ali / Anna Hatke Matrix (insert letter in column below)Preservative (insert # in box to the right) - -2 For EPA Staff Only: If after A Drinking waterF. Soil / Sediment / Solid 1. Ice6. NaOH p#h .1 2Analysis Requestedhours or nobody is available, B. Ground / Well water G. Oil / Solvent / Liquid2.F50pH of< 2 7. Ascorbic Acidleave cooler(s) and signed COC C. Storm waterH. Soil Gas3. HNO3, PH>2: 8. MeOHin the walk - in fridge (Room 190A) D. Surface water1. Air Canister4. HCl, pH>29. Otherand notify appropriate staff for NO2N03 E. WastewaterJ5. NaSO10. Other Containers /next day check - in. Sample IDDate Time Comp Grab MatrixAmmo ST$ ATSSSample Specific Comments GF01 05/22/2024 09:50 E GF02 05/22/2024 4 10:13 E 3 GF04 05/22/2024 10:54 E GGFF0035 0055//2222//2022042 41 01:13:317E mEm WWmW mm .. Relinquished by: (Signature)Received by: (Signature) DateTimeRemAq arks Ali, Nafisah DigDaite:t 2a02l405l.2y2 18:s57i:2g3 -n04e'0d0 ' by Ali, NafisahLauraGlowacko (ESAT) 5/23/24 8:58 CAFO samples collected at Relinquished by: (Signature)Received by: (Signature)DateTime different farms. Distribution: Original Accompanies Shipment. Copy to Coordinator Field Files,FSBFORM CHAINCUSTODY1 Revision 1 ED_019088A_00004944-00018Relinquished by: (Signature)Received for Laboratory by: (Signature)[Date]Jime 05/16/2022 ED_019088A_00004944-00018 Page 7 of 7 US EPA REGION 1 SAMPLE RECEIPT CHECKLIST PROJ #: 24050033RECEIPT DATE: 5/23/24 LSOUCRAVTEIYO NN:A ME: Concentrated Animal Feeding OperationREC'D BYL:a ura Glowacki (ESAT). OSC / PO: Nafisah AliSITE ID:SUPERFUND: N - WERE SAMPLES SHIPPED?COMMENTS: TRACKING #: DATE / SENTWastewater samples NO Hand Delivered COOLER TEMPERATURE UPON ARRIVAL 21 C / NA 9 $ TNL CHAIN OF CUSTODY PRESENT? Y9 AMMO COMPLETE? Y 9 TP CUSTODY SEALS PRESENT ON COOLER?N SAMPLES? N WERE SAMPLE CONTAINERS INTACT? 9 TSS 8. $ STA WAS SAMPLE PRESERVATION DOCUMENTED?YSamples relinquished to walk in COCSample Containeron 5/22/24 by Nafisah Ali and recieved by Laura Glowacki on APPROPRIATE SAMPLES VOLUME5/23/24 FOR REQUESTED ANALYSIS?Y SAMPLES AND COC MATCH?Y IF ANY PROBLEMS WAS PROJECT MANAGER NOTIFIED? BY WHOM? NA Y APPROPRIATE SAMPLE CONTAINERS? : SAMPLES WITHIN HOLDING TIMES?Y ALL ANALYSIS SPECIFIED ON COC?Y DATE / TIME OF COLLECTION ON COCY TURN - AROUND TIME: 4 weeks ED_019088A_00004944-00019 United States Environmental Protection Agency EPALaboratory Services and Applied Science DivisionPage 1 of 18 Region 1, New England11 Technology Drive North Chelmsford, MA 01863-2431 June 24, 2024 Laboratory Report Nafisah Ali US EPA Region 1 Project Number: 24050033. Project: Concentrated Animal Feeding Operation Sampling Inspectic Analysis: Total Nitrogen EPA Chemist: Paul Toompas Date Samples Received by the Laboratory: 05/23/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log - in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP - NO2 - NO34. Combined nitrate and nitrite will be reported as nitrogen. Total nitrogen (the sum of nitrate - nitrogen, nitrite - nitrogen, ammonia - nitrogen and organically bonded nitrogen) will be reported as nitrogen. Samples were analyzed according to LSBSOP - NO2 - NO34, which is currently under review. Samples were analyzed on a SEAL AQ300 Discrete Analyzer. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8340. Sincerely, DANIEL Digitally signed by DANIEL BOUDREAU BOUDREAU Date: 2024.06.2424050033 $ TNL 10:42:43 -04'00 ' ED_019088A_00004944-00020 Qualifiers: RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J * Estimated value J1-Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E = Estimated value exceeds the calibration range L = Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P = The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC / MS. A = Suspected Aldol condensation product. N = Tentatively identified compound. ANR Analysis not required. Page 2 of 18 24050033 $ TNL ED_019088A_00004944-00021 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 3 of 18 Concentrated Animal Feeding Operation Sampling Inspection Total Nitrogen Client Sample ID:GF01Lab Sample ID:AC13039 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:10 Wet Weight Prepared:N / ApH:> 2 Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compound ug / L ug / L Qualifier Combined Nitrate & Nitrite3400400J Comments: Sample not preserved to a pH less than 2. Value determined from 10x dilution. Reporting limit raised accordingly. 24050033 $ TNL ED_019088A_00004944-00022 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 4 of 18 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Blank for Nitrogen Client Sample ID:N / ALab Sample ID:N / A Date of Collection:N / AMatrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:N / A Wet Weight Prepared:N / ApH:n / a Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compound ug / L ug / L Qualifier Combined Nitrate & NitriteND40 24050033 $ TNL ED_019088A_00004944-00023 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 5 of 18 Concentrated Animal Feeding Operation Sampling Inspection Total Nitrogen Client Sample ID:GF02Lab Sample ID:AC13040 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:10 Wet Weight Prepared:N / ApH:> 2 Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compound ug / L ug / L Qualifier Combined Nitrate & Nitrite830400J Comments: Sample not preserved to a pH less than 2. Value determined from 10x dilution. Reporting limit raised accordingly. 24050033 $ TNL ED_019088A_00004944-00024 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 6 of 18 Concentrated Animal Feeding Operation Sampling Inspection Total Nitrogen Client Sample ID:GF03Lab Sample ID:AC13041 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:10 Wet Weight Prepared:N / ApH:> 2 Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compoundug / L ug / L Qualifier Combined Nitrate & Nitrite1500400J Comments: Sample not preserved to a pH less than 2. Value determined from 10x dilution. Reporting limit raised accordingly. 24050033 $ TNL ED_019088A_00004944-00025 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 7 of 18 Concentrated Animal Feeding Operation Sampling Inspection Total Nitrogen Client Sample ID:GF04Lab Sample ID:AC13042 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:1 Wet Weight Prepared: N / ApH: Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compound ug / L ug / L Qualifier Combined Nitrate & NitriteANR40 Comments: Sample not suitable for analysis due to color and inability to filter. 24050033 $ TNL ED_019088A_00004944-00026 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 8 of 18 Concentrated Animal Feeding Operation Sampling Inspection Total Nitrogen Client Sample ID:GF05Lab Sample ID:AC13043 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:6/12/2024Amount Prepared: N / A Date of Analysis:6/12/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:1 Wet Weight Prepared:N / ApH:< 2 Volume Extracted:N / AGPC Factor:N / A Final Volume:N / A Concentration RL CAS Number Compound ug / L ug / L Qualifier Combined Nitrate & NitriteANR40 Comments: Sample not suitable for analysis due to color. 24050033 $ TNL ED_019088A_00004944-00027 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 13 of 18 Concentrated Animal Feeding Operation Sampling Inspection MATRIX SPIKE (MS) RECOVERY Sample ID: AC13039 SPIKE SAMPLE MS MS QC ADDED CONCENTRATION CONCENTRATION% LIMITS PARAMETER ug / L ug / L ug / L REC (% REC) Combined Nitrate & Nitrite 400 3440 4010 R 80-120 24050033 $ TNL ED_019088A_00004944-00028 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 14 of 18 Concentrated Animal Feeding Operation Sampling Inspection MATRIX SPIKE DUPLICATE (MSD) RECOVERY Sample ID: AC13039 MSD MSD MSD RPD QC SPIKE CONCENTRATION%% LIMITS PARAMETER ADDED ug / L REC RPD Combined Nitrate & Nitrite 400 4440 R 10.2 20 24050033 $ TNL ED_019088A_00004944-00029 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 15 of 18 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Duplicate Results Sample ID: AC13039 SAMPLE SAMPLE DUPLICATE PRECISION RESULT RESULT RPD QC PARAMETER. ug / L ug / L% LIMITS Combined Nitrate & Nitrite 3440 3280 5 20 24050033 $ TNL ED_019088A_00004944-00030 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 16 of 18 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Fortified Blank (LFB) Results LFB AMOUNT LFB LFB QC SPIKED RESULT RECOVERY LIMITS PARAMETER ug / L ug / L%% Combined Nitrate & Nitrite4003939890-110 Comments: Samples in Batch:AC13039, AC13040, AC13041, AC13042, AC13043, AC13044, AC13045, AC13046, AC13047 24050033 $ TNL ED_019088A_00004944-00031 Page 17 of 18 ** US EPA Region 1CHAIN OF CUSTODY RECORDPage: of 11 Technology Drive, N. Chelmsford, MA 01863 [CPA Project NosProject Name & Lacation:Sampler's Name and Signature: 24050033 Concentrated Animal Feeding Operation (CAFO) Sampling Inspection Nafisah Ali / Anna Hatke Matrix (insert letter in column below)Preservative (insert # in box to the right)2 For EPA Staff Only: If after A. Drinking waterF. Sall / Sediment / Solid1. ce6. NaOH# plAnalysis Requestedhours or nobody is available, B. Ground / Well waterG. OI / Solvent / Liquid2. H.SO,, pofH < 27. Ascorbic Acidleave cooler(s) and signed COC C. Storm waterH. Soll Gas3. HNO3, pH < 28. MeOH.in the walk - in fridge (Room 190A). D. Surface water1. Air Canister4. HCl, pH < 29. Otherand notify appropriate staff for NO2N03 E. Wastewater5. NaSO,10. Other/ Containersnext day check - in: Ammo TSS SSTA Sample (DDateTime Comp Grab MatrixSample Specific Comments . GF01 05/22/2024 09:50 E w 3 / GF02 05/22/2024 10:13 E w 3. / GF0305/22/2024 10:33 3 E 3 GGFF0045005/52/22/22/02240 2140 :5141:E17wwwE 33SSamapmlpelse wse rceo lclolelcetcetded ffrroomm mlaenaucreh aptiet Relinquished by: (Signature)Received by: (Signature)DateTimeRemarks: Ali,NafisahDigDaite:t 2a024l.0l5.2y2 18:s 5i7: g23n-04e'0d0 ' by Ali, NafisahLaura Glowacko (ESAT) 5/23/24 8:58 CAFO samples collected at Relinquished by: (Signature)Received by: (Signature)DateTime different farms. Relinquished by: (Signature)Received for Laboratory by: (Signature)DateTime. Distribution: Original Accompanies Shipment. Copy to Coordinator Field Files.FSBFORM - CHAINCUSTODY: Revision 1 05/16/2022 ED_019088A_00004944-00032 US EPA REGION 1 SAMPLE RECEIPT CHECKLIST PROJ #: 24050033RECEIPT DATE: 5/23/24 SURVEY NAME: Concentrated Animal Feeding OperationREC'D BY: LOCATION: Laura Glowacki (ESAT) OSC / PO: Nafisah AliSITE ID:SUPERFUND: N WERE SAMPLES SHIPPED?COMMENTS: TRACKING #: DATE / SENT:Wastewater samples NO. Hand Delivered COOLER TEMPERATURE UPON ARRIVAL 2 C / NA 9 STNL CHAIN OF CUSTODY PRESENT? Y9 AMMO COMPLETE?9 TP CUSTODY SEALS PRESENT ON COOLER?N SAMPLES? WERE SAMPLE CONTAINERS INTACT?Y 9 TSS 8 $ STA WAS SAMPLE PRESERVATION DOCUMENTED?YSamples relinquished to walk in COCSample Containeron 5/22/24 by Nafisah Ali and recieved by Laura Glowacki on APPROPRIATE SAMPLES VOLUME5/23/24 FOR REQUESTED ANALYSIS?Y SAMPLES AND COC MATCH?Y IF ANY PROBLEMS WAS PROJECT MANAGER NOTIFIED? BY WHOM? Y APPROPRIATE SAMPLE CONTAINERS? SAMPLES WITHIN HOLDING TIMES? ALL ANALYSIS SPECIFIED ON COC? DATE / TIME OF COLLECTION ON COCY TURN - AROUND TIME: 4 weeks Page 18 of 18 ED_019088A_00004944-00033 United States Environmental Protection Agency EPALaboratory Services and Applied Science DivisionPage 1 of 15 Region 1, New England11 Technology Drive North Chelmsford, MA 01863-2431 June 10, 2024 Laboratory Report Nafisah Ali US EPA Region 1 Project Number: 24050033 Project:Concentrated Animal Feeding Operation Sampling Inspection Analysis: HPLC / MS / MS Source Tracking Analysis EPA Chemist: Peter Philbrook Date Samples Received by the Laboratory: 05/23/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log - in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP - LCMS_STA3. The SOP is based on an EPA Regional Analytical Method developed at the EPA New England Laboratory. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8340. Sincerely, DANIEL Digitally signed by DANIEL BOUDREAU BOUDREAU Date: 2024.06.1024050033 $ STA 09: 08: 18-04'00 ' ED_019088A_00004944-00034 Qualifiers: RL Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value J1 = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E Estimated value exceeds the calibration range L Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P = The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC / MS. A = Suspected Aldol condensation product. N = Tentatively identified compound. Page 2 of 15 24050033 $ STA ED_019088A_00004944-00035 U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCHPage 3 of 15 Concentrated Animal Feeding Operation Sampling Inspection HPLC / MS / MS Source Tracking Analysis Client Sample ID:GF01Lab Sample ID:AC13039. Date of Collection:5/22/2024.Matrix:Wastewater Date of Preparation:5/28/2024Amount Prepared: 500 mL Date of Analysis:6/04/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:1 Wet Weight Prepared:N / ApH:N / A Concentration RL CAS Number Compound ng / L Dilution ng / L Qualifier 486-56-6 Cotinine 1.0 I 0.40 103-90-2 Acetaminophen 16 1 2.0 611-59-6 Paraxanthine 19 2.0 29122-68-7 Atenolol ND 2.0 58-08-2 Caffeine 15 4.0 56392-17-7 Metoprolol ND 1 2.0 58-73-1 Diphenhydramine ND 0.40 298-46-4 Carbamazepine ND 0.40 Surrogate CompoundsRecoveries (%)QC Ranges Phentermine - D555 37-113 Diphenhydramine - D361 8-114 Comments: 24050033 $ STA ED_019088A_00004944-00036 U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION I LABORATORY SERVICES BRANCHPage 4 of 15 Concentrated Animal Feeding Operation Sampling Inspection HPLC / MS / MS Source Tracking Analysis Client Sample ID:GF02Lab Sample ID:AC13040 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:5/28/2024Amount Prepared: 500 mL Date of Analysis:6/04/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:1 Wet Weight Prepared:N / ApH:N / A Concentration RL CAS Number Compound ng / L Dilution ng / L Qualifier 486-56-6 Cotinine 2.2 1 0.40 103-90-2 Acetaminophen 5.7 I 2.0 611-59-6 Paraxanthine 4.7 1 2.0 29122-68-7 Atenolol ND 1 2.0 58-08-2 Caffeine 7.8 1 4.0 56392-17-7 Metoprolol ND 1 2.0 58-73-1 Diphenhydramine ND 1 0.40 298-46-4 Carbamazepine ND 0.40 Surrogate CompoundsRecoveries (%)QC Ranges Phentermine - D553 37-113 Diphenhydramine - D331 8-114 Comments: 24050033 $ STA ED_019088A_00004944-00037 U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCHPage 5 of 15 Concentrated Animal Feeding Operation Sampling Inspection HPLC / MS / MS Source Tracking Analysis Client Sample ID:GF03Lab Sample ID:AC13041 Date of Collection:5/22/2024Matrix:Wastewater Date of Preparation:5/28/2024Amount Prepared: 500 mL Date of Analysis:6/04/2024Percent Solids:N / A Dry Weight Prepared:N / AExtract Dilution:1 Wet Weight Prepared: N / ApH:N / A Concentration RL CAS Number Compound ng / L Dilution ng Qualifier 486-56-6 Cotinine 2.2 0.40 103-90-2 Acetaminophen 12 2.0 611-59-6 Paraxanthine 6.0 1 2.0 29122-68-7 Atenolol ND 1 2.0 58-08-2 Caffeine 9.1 1 4.0 56392-17-7 Metoprolol ND 1 2.0 58-73-1 Diphenhydramine ND 1 0.40 298-46-4 Carbamazepine ND 1 0.40 Surrogate CompoundsRecoveries (%)QC Ranges Phentermine - D554 37-113 Diphenhydramine - D351 8-114 Comments: 24050033 $ STA ED_019088A_00004944-00038 ** PROTECTION Analytical Report Narrative HPLC / MS / MS Source Tracking Analysis ($ STA) Project Number: 24050033-CAFO -VT Farms Page 13 of LIMITED 15 STATES WWENDAMENTAL MOTECTION Analyst: Peter Philbrook Date: 05-JUN-2024 Analysis Code: $ STA (Source Tracking Analysis) Sample extracts were all a yellow tan color and foamed. Two samples could not be extracted. One was from a manure lagoon, the other was a manure leachate. Impossible to pass through SPE cartridge. GF04 / AC13042 manure pit GF05 / AC13043 manure leachate. Clients were notified via email. Routine analysis otherwise ED_019088A_00004944-00039 SPATRO STATPage 14 of 15 US EPA Region 1CHAIN OF CUSTODY RECORDPage:of 11 Technology Drive, N. Chelmsford, MA 01863 PAL CPA Project HosProject Name & Location:(Sampler's Name and Signature. 24050033 Concentrated Animal Feeding Operation (CAFO) Sampling Inspection Nafisah Ali / Anna Hatke Matrix (insert letter in column below)Preservative (insort # in box to the right)2 For EPA Staff Only: If after A. Drinking waterF. Soll / Sediment / Solid1. Ice6. NACH# orAnalysis Requestedhours or nobody is available, B. Ground / Well waterG. OI / Solvent / Liquid2. HSO..of pH < 27. Ascorbic Acidleave cooler(s) and signed COC C. Storm WaterH. Soil Gas3. HNO3, pH < 28. MeOHin the walk - in fridge (Room 190A) D. Surface water1. Air Canister4. HCl, pH < 29. Otherand notify appropriate staff for NO2N03 / E. Wastewater2.10. Other 5. Na2S2O3Containersnext day check - in. Ammo TSS SSTA Sample IDDateTime Comp Grab MatrixSample Specific Comments GF01 05/22/2024 09:50 E 3 GF02 05/22/2024 10:13 E 3 0000 GF03 05/22/2024 10:33 E 3 GF0405/22/2024 10:54E3Samples were collected from manure pit GF0505/22/2024 11:17E3Sam Samples collected from leachate Relinquished by: (Signature)Received by: (Signature)[DateRemarks Ali, NafisahDiDgatei: t20a24.l05l.22y 1 8:5s7:i23 g-0n4'e00 d by Ali, NafisahLucGlowacks (ESAT) 5/23/24 8:58 CAFO samples collected at Relinquished by: (Signature)Received by: (Signature)DateTime different farms. Relinquished by: (Signature)Received for Laboratory by: (Signature)Date Distribution: Original Accompanies Shipment. Copy to Coordinator Field Files.FSBFORM - CHAINCUSTODY1 Revision 1 05/16/2022 ED_019088A_00004944-00040 US EPA REGION 1 SAMPLE RECEIPT CHECKLIST PROJ #: 24050033RECEIPT DATE: 5/23/24 SURVEY NAME: Concentrated Animal Feeding OperationREC'D BY: LOCATION: Laura Glowacki (ESAT) osc / Po: Nafisah AliSITE ID:SUPERFUND: N WERE SAMPLES SHIPPED?COMMENTS: TRACKING #: DATE / SENT:Wastewater samples Hand Delivered NO. COOLER TEMPERATURE UPON ARRIVAL 2 C / NA 9 $ TNL CHAIN OF CUSTODY PRESENT? 9 AMMO COMPLETE?Y9 TP CUSTODY SEALS PRESENT ON COOLER?N SAMPLES? N WERE SAMPLE CONTAINERS INTACT?Y 9 TSS 8.SSTA WAS SAMPLE PRESERVATION DOCUMENTED?YSamples relinquished to walk in COC-Sample Containeron 5/22/24 by Nafisah Ali and APPROPRIATE SAMPLES VOLUME recieved by Laura Glowacki on 5/23/24 FOR REQUESTED ANALYSIS?Y SAMPLES AND COC MATCH?Y IF ANY PROBLEMS WAS PROJECT MANAGER NOTIFIED? BY WHOM? Y APPROPRIATE SAMPLE CONTAINERS? SAMPLES WITHIN HOLDING TIMES? ALL ANALYSIS SPECIFIED ON COCYY DATE / TIME OF COLLECTION ON COCY TURN - AROUND TIME: 4 weeks Page 15 of 15 ED_019088A_00004944-00041 United States Environmental Protection Agency EPALaboratory Services and Applied Science DivisionPage 1 of 8 Region 1, New England11 Technology Drive North Chelmsford, MA 01863-2431 June 24, 2024 Laboratory Results Nafisah Ali US EPA Region 1 Project No:24050033 Project:Concentrated Animal Feeding Operation Sampling Inspectic Analysis:Total Phosphorus in Water as P EPA Chemist:Holly Westbrook Date Samples Received by the Laboratory: 05/23/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log - in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP - OPHOS - TP13. The SOP is based on the AQ300 Method EPA-119-D Rev. 3A " Phosphorus - P, Total, in Surface and Saline Waters, Domestic and Industrial Wastes " and EPA method 365.1 " Determination of Phosphorous by Semi - Automated Colorimetry ", Revision 2.0, August 1993. Samples were analyzed on a SEAL AQ300 Discrete Analyzer. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. If you have any questions please call me at 617-918-8340. Sincerely, DANIEL Digitally signed by DANIEL BOUDREAU BOUDREAU Date: 2024.06.2424050033TP 11: 19: 07-04'00 ' ED_019088A_00004944-00042 Qualifiers: Page 2 of 8 RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value J1 = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E = Estimated value exceeds the calibration range L Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC / MS. A = Suspected Aldol condensation product. N = Tentatively identified compound. ANR Analysis not required. 24050033TP ED_019088A_00004944-00043 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Concentrated Animal Feeding Operation Sampling Inspection Page 3 of 8 Total Phosphorus in Water as P Matrix:Wastewater Concentration RL Sample Number Lab ID Collected Extracted Analysisug / L ug / L Qualifier GF0IAC13039 05/22/2024 9:50 06/17/2024 06/17/2024770 120 J Comments: Result determined from 10x dilution, reporting limit raised accordingly. Sample not preserved, results qualified. GF02AC13040 05/22/2024 10:13 06/17/2024 06/17/20241600 120 J Comments: Result determined from 10x dilution, reporting limit raised accordingly. Sample not preserved, results qualified. GF03AC13041 05/22/2024 10:33 06/17/2024 06/17/20241600 120 Comments: Result determined from 10x dilution, reporting limit raised accordingly. Sample not preserved, results qualified. GF04AC13042 05/22/2024 10:54 06/17/2024 06/17/2024160000 18000 J Comments: Result determined from 1,500x dilution, reporting limit raised accordingly. Sample not preserved, results qualified. Digestion filtered due to suspended solids. GFOSAC13043 05/22/2024 11:17 06/17/2024 06/17/202412000 1600 J Comments: Result determined from 130x dilution, reporting limit raised accordingly. Sample not preserved, results qualified. Blank06/17/2024 06/17/2024 ND 12 Comments: 24050033TP: ED_019088A_00004944-00044 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Concentrated Animal Feeding Operation Sampling Inspection MATRIX SPIKE (MS) Results Page 4 of 8 SPIKE SAMPLE MS MS OC ADDED CONCENTRATION CONCENTRATION% LIMITS SAMPLE ID PARAMETERug / Lug / Lug / L REC (% REC) AC13040Total Phosphorus in Water as P8001551233497.983-119 Comments: MATRIX SPIKE DUPE (MSD) RESULTS MSD MSD MSD RPD QC SPIKE CONCENTRATION%% LIMITS SAMPLE ID PARAMETER ADDED ug / L REC RPD AC13040Total Phosphorus in Water as P800224086.120 Comments: 24050033TP ED_019088A_00004944-00045 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORY Page 5 of 8 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Duplicate Results SAMPLE SAMPLE DUP PRECISION QC RESULT RESULT RPD LIMITS SAMPLE ID PARAMETER ug / L ug / L% (% RPD) AC13040 Total Phosphorus in Water as P 1551 1662 7 20 24050033TP ED_019088A_00004944-00046 US ENVIRONMENTAL PROTECTION AGENCY NEW ENGLAND LABORATORYPage 6 of 8 Concentrated Animal Feeding Operation Sampling Inspection Laboratory Fortified Blank (LFB) Results LFB AMOUNT LFB LFB QC SPIKED RESULT RECOVERY LIMITS PARAMETER ug / L ug / L%% Total Phosphorus in Water as P8082.8103.590-110 Samples in Batch: AC13039, AC13040, AC13041, AC13042, AC13043, AC13044, AC13045, AC13046, AC13047 24050033TP ED_019088A_00004944-00047 Page 7 of 8 US EPA Region 1CHAIN OF CUSTODY RECORDPage:of 11 Technology Drive, N. Chelmsford, MA 01863 CPA Project No:Project Name & Location:Sampler's Name and Signature: 24050033 Concentrated Animal Feeding Operation (CAFO) Sampling Inspection Nafisah Ali / Anna Hatke Matrix (insert letter in column below)Preservative (insert # in box to the right) -2 For EPA Staff Only: If after A. Drinking waterF. Soil / Sediment / Solid1. Ice6. NaOH, p# H: 12Analysis Requestedhours or nobody is available, B. Ground / Well waterG. Oil / Solvent / Liquid2. HSO,of pH < 27. Ascorbic Acidleave cooler(s) and signed COC C. Storm Water:H. Soil Gas3. HNO3. pH < 28. MeOHin the walk - in fridge (Room 190A) NO2N03 D. Surface water1. Air Canister4. HCl, pH>29. Otherand notify appropriate staff for / E. WasStaemwpalet eIrD5D.at eN aTime SComp0 G,ra1b0 .M atOrtihxerAmmCoo nTtSaS iSnSeTrAsSanmepxlte Sdpaeyc icfhico cCko m-m enitns. . GF01 05/22/2024 09:50 E 3 GF02 05/22/2024 10:13 E 3 wwww GF03 05/22/2024 10:33 E 3 GF0405/22/2024 10:54E3Samples were collected from manure pit GF0505/22/2024 11:17E3Samples collected from leachate Relinquished by: (Signature)Received by: (Signature)DateTimeRemarks Ali, NafisahDigDaitet: 2a02l4.l05.y22 1s8:5i7:g23n 04e'0d0 ' by Ali, NafisahLaura Glowacko (ESAT) 5/23/24 8:58 Relinquished by: (Signature)Received by: (Signature)DateTime CAFO samples collected at different farms. Relinquished by: (Signature)Received for Laboratory by: (Signature)DateTime Distribution: Original Accompanies Shipment. Copy to Coordinator Field Files:FSBFORM CHAINCUSTODY! Revision 1 05/6/2022 ED_019088A_00004944-00048 US EPA REGION 1 SAMPLE RECEIPT CHECKLIST PROJ #: 24050033Receipt date: 5/23/24 SURVEY NAME: Concentrated Animal Feeding OperationREC'D BY: LOCATION: Laura Glowacki (ESAT) osc / Po: Nafisah AliSITE ID:SUPERFUND: N WERE SAMPLES SHIPPED?COMMENTS: TRACKING #: DATE / SENT:Wastewater samples NO. Hand Delivered COOLER TEMPERATURE UPON ARRIVAL 20 C / NA 9 STNL CHAIN OF CUSTODY PRESENT? 9 AMMO COMPLETE? Y 9 TP CUSTODY SEALS PRESENT ON COOLER?N SAMPLES? WERE SAMPLE CONTAINERS INTACT?Y 9 TSS 8 $ STA WAS SAMPLE PRESERVATION DOCUMENTED?YSamples relinquished to walk in COCSample Containeron 5/22/24 by Nafisah Ali and APPROPRIATE SAMPLES VOLUME recieved by Laura Glowacki on 5/23/24 FOR REQUESTED ANALYSIS?Y SAMPLES AND COC MATCH?Y IF ANY PROBLEMS WAS PROJECT MANAGER NOTIFIED? BY WHOM? Y APPROPRIATE SAMPLE CONTAINERS? SAMPLES WITHIN HOLDING TIMES? ALL ANALYSIS SPECIFIED ON COC7Y DATE / TIME OF COLLECTION ON COCY TURN - AROUND TIME: 4 weeks Page 8 of 8 ED_019088A_00004944-00049