Document 10rragR7LQq3DDKBgrO9knm7j
BIOACCUMULATION
TEST SUBSTANCE
Identity: N-ethylperfluorooctane sulfonamidoethanol; may also be referred to as N1.-1E,t2,F2O.S3.E3,A4l,c4o,h5o,l5,o6r,6F,M1-.374,82,28.,8-(h1e-pOtcatdaenceafsluulofroon-aNm-i(d2-eh,ydNr-oextyheytlh-yl)-,
CAS # 1691-09-2)
Remarks: Material is an off-white, waxy solid of uncharacterized purity.
METHOD:
METxeyttprheao:cdtAiinoganuliaydnseidlsiaonnfaeltyfisosilssuleopwfreoodrc:feldNuuoorrneoeschgweiemvrieecnadlfeofrvrisosamemdpilnbidyni3ggMeon.fotuhsefoisrhgacnaiusgmhst.in the Tennessee River above and below the Wheeler Dar.
GLP (YN): No
Year: 1979
oRregmaanriksmssfiferlodm: tThheeTreeninsensoseinefoRrimvaetrinoneaorntshaemmpalniunfgacptruorciendgurfaecsiloitfy.the
Four fish
Wheeler
were
Dam,
caught an
one white
d utiized as samples; Two channel catfish
bass caught below and one white crappie
caught
caught
above
above
Wheeler Dam. Wheeler Dam is approximately 26 nautical miles downstream
fmraonmythmeil3esM aDbeocvaetuarndPlabnetldewf.ftluheentdadimscthhaergfies.h wItewraescanuogthtn.oted in the report how
Amtloefn app10m0sptapnmdasrtdanodfaFrMd-(3in42et2hy(lNa-cEetFtOaStEet)oalmcaohrokl)wiwtahsetphryelpaacreetdatbey idnialut1i0ngm1l
volumetric flask.
wr
YT
Ochnaennwehlolcaetfcihsahnwnealscdaitsfsisehctweadsanhdomtohgeevnairzieoudstioncdirveiadtuealonpaertssamwpelree. The other homogenized to create individual samples.
The white bass had a 6.3 cm i.d. dinker die core sample taken just off the lateral
line behind the gill plate making up a 20.591 g sample containing skin, filet, small part of the backbone, reproductive organs, partof the kidney, and rectum.
Tchreeawtheiate16c.r6a8p4pigerhaamdsaamdpinlkeercodniteacionirnegsfatm,plveerttaekbernaeb,ehsiinndatnhed gbiil .plate to
UNURanHRg 006286 IA <3 All samples were homogenized in known volumes of DI water and divided into
3 five aliquots each. &EPa-oTs 0008118080
Samples were centrifuged, extracted organic and inorganic fluoride.
with
ethyl
acetate,
and
analyzed
by
GC
for
It was noted that FM-3925 (N-MeFOSE alcohol) and FM-3422 (N-EtFOSE alcohol) could not be distinguished with GC with electron captureparameters. The results are therefore a combined value.
RESULTS
N-MeFOSE alcohol/ N-EtFOSE alcohol Concentration In Tennessee River Fish by GC
N.EFOpSpEma)lcohol RT S-- --T -- -- i-- --
[E Wit Basscoresample{r -- 35-- 1 T TTET -- Y -- I -- -- -- T Lo Le Y -- -- -- --
Upon completion of GC analysis, there was concern that the values were not
definitive. Additional
done using Capillary
GanaaslyCshirsoofmaettohyglraacpehtyatwiethh oEmloecgternoantCeapextturraectawndasGtCheunsing
a
Microwave Sustained Helium Plasma Detector.
Qualitative analysis of the fish extracts using Capillary Gas Chromatography with
electron capture failed to identify the presence of N-EtFOSE alcohol. This was further supported by analysis by a Microwave Sustained Helium Plasma Detector where again, no evidenceof any fluorochemicals were detected andspecifically no N-EtFOSE alcohol and no N-EtFOSA (F-6309) were detected. The results obtained by the microwave plasma detector on spiked samples indicated that NEtFOSE alcohol, if present, could have been detected from its fluorine content at 0.1 ppm in the ethyl acetate extracts. No reference was made to N-MeFOSE alcohol except for the general statement made "No fluorocarbon peaks were observed in the actual samples."
Rbreomuagrhktsu:pTahfteerortihgeinraelporretpowrat s(5g/e2n2e/7r9a)terdeftehraetnFceMd-3a9s2a3mapnlde
for FM-3923.
FM-3422 are
It was
both
the same coumpound (N-EtFOSE alcohol). Analytical was conducted to verify its
006287
identity and No. 7238 --
DitetwearsmifnoautnidontoofbeFlNu-orEitnFatOeSdAAl(cF-o6h3o0l9s).in
The Fish
aEtxttaracchtesd",re1p0o/r2t3/(7`9A)R
stil
refers report
taolsFoMh-a3s92i3ncownhseisnteinn cfiaecst.thTehsealmapslt epalraabeglreadphasinFdMic-a3t9es23thies
F-6309. abilty to
This detect
iflnudoircianteescoantdeenttecattio0n.1lipmiptmofle0v.e5l.ppHmo.wever, a review of the procedure used
hThoemofgirestnaretpeorbty (g5a1s22c/h7r9)omdaetsocgrirbaepshyan(aGlCys)iwsiotfh eetlheycltraocnetcaatpetuexrterdaecttescotifofni.shThe
analysis retention
shows times
the presence of identical to both
materials in N-MeFOSE
afilschohtoilssaunedexNt-raEcttFs OtShaEt
have GC alcohol and
to N-EtFOSA. The GC retention times of N-MeFOSE alcohol and N-E{FOSE
alcohol standards were the same (not resolved) by the method used in the first
erexptorratc.ts,Thbuetfeirlsetcrterpoonrtcaipntduirceatdeesttehcetiporneissennoctesopfecfilfuiocfroorcfhleumoircoaclsheimnitchaelsf.ishThus
the results reported in the first report were not a specific identification of
fluorochemicals detectable by GC. (Report 1 also has errors in column 4 of
Tsahboulled1.beIncthhaeng1eBdrotow,0.00.64.0) should be changed to 4.13, and in the 2A row, 0.004
The second report (12/28/79) shows a misinterpretation in the first report, It
includes a descriptionof GC analyses of ethyl acetate extractsoftwoof the samples described in report 1 samples 1B and 3A. The work described irn eport
fs2luuusostraeiindneeemdloehcdteerl,oinuMmcSappHltPausDrmeamdeedtteehtceotcditoiinosnaspn(edMciSrfHeifcPeDfro)erfnilcnueotsrhiaenerf.elupooTrrhitneeusMainSndgHsPmuiDlcfrurroeswmuaolvdtese.shIonw aincnodeitfcalauttoeirneogxctthrheaamctitcFsa-al6ts3l0ien0stsahntedheatnNh-y0lE.1tacFpeOptSmaE.teaIelnxctotrhhaoecltfsi(.rFstM-rTe3hp4eo2rt2r,e)sNual-rteEstpaFrreOesSeiAnntt(eFirn-p6rte3ht0ee9de)taahsynld bNe-MpreeFsOenStEraelspceochtoilv(elFyM-a3t902.58)2 /anNd-E3t.3F1OSpEpmalcinohsoalm(pFlMe-13B42a2n)dhaatd1a.p4p8eaanrded0.t8o0 npoptmtoinhsaavmepbleee3nA.fluTohruosc,hetmhiecaGlCs-aabnldetchuosmpcoouulnddnsotseheanveinbteheenfiNrs-t MreepFoOrtSaEppear alcohol, N-EtFOSE alcohol or N-EtFOSA.
The first report shows the presence of unidentified organic fluorine and of
inorganic fluorine in the fish tissue.
report.
This was not re-evaluated in the second
CONCLUSIONS
No reliable conclusions can be derived from this study. Submitter: 3V Company, Environmental Laboratory, P.O. Box 33331, St. Paul,
Minnesota, 55133
006288
DATA QUALITY
dReelsiiagbniliintyr:elaKtiiiomnitsochthreanouktifnagll3a.nWditshaomuptlianng upnodinetrss,tavenrdiifniagbloefdtahteasaomnptlhiengactual
a`cnodncfernotrmantaitounrsoalffslouuorrcoesc,heamctiicvailtisesininthteherimvaernuffraocmtubroitnhgthfaecimlaitnyupfraicotrutroisnagmfpalciilnigty,
any applicable environmental conditions (e.g. rain evens), and a clear
ulintdleertsotbaendcionngcloufdheod.w Aldodnigttiohnealslaym,ptlheedanfaislhytwiecarledianttaheconsfaimipctlsi.ngItacraenan,otthebree is
adenfailnyittiicvaellymceotnhcolduodleodgywhwiecrheannoatlyvtailciadladteadt.a
set is State
correct. units of
Extraction and results is ppm.
Itis
not
correlated to mg analyte per kg body weight. Identity and purity of reference
bcaosmipsoiunnfdacst.was not established therefore stated analyte concentrations have no
REFERENCES
3JaMmTeeschE.niGcaalgnRoenp,orPtro"jBeicotac7c8u-m2u7l4a0t,ioDnecoaftuFrl,uoArloacbheammiaca--lTseinnnTeesnsne.eRiRvievrerFiFsihs.h", Report Number 001, May 22, 1979
3FiMshTeExcthrnaicctasl."ReDp.orF.t H"aAgRenNo,.P7ro2j3e8ct~AD0e0t0e0r0m7i,naEtnivoinorofnmFelunotrailnaEtnegdinAelecroihnoglsainnd Pollution Control, Report Number238, October 23, 1979.
G3aMgnTeocnh,niPcraoljeRcetp7o8r-t27`4F0l,uoDreoccahteumri,caAllsaibnaTmean--neTsesneneesRsieveerRFiivseh.r"FJisahm,eRsepEo.rt Number 100, December 28, 1979
3CoMmerlelquUensitveedrseitxyp,erMtaorvcehrv8i,e1w9,9"3Bioaccumulation Studies", Dr. James Gillet,
OTHER
Last changed: 5/18/00
006289
=;
- Teckdcdp k heron summany
-rams
To TEHCAL conmmsmcarions cen - 300
im--goran = oc wiv anbo soa, 1m coi 0 TCE)
DECer201978
DEencvaitruorn,menAtlaalbemLsab-orTaetmosrsyss(eEsE g&ivPCe)r rian
T0e22o2 r
Btosccumulation of iverochesicals tn Tess. River rian | oor
sD.ameL.n 1B.acconognon
Jit
Ties
1568
Leb teguart #871
--
mamrd BED rs Ruri |HERD Few
=EAeumpseEa, [mTr[feTTaromaamoiessmtncsvmREeiovQeuranliA itvaoltraieveondanedorqtutoanvtiaiteatnitvdeeednteEteeT1rr2mmitnihatnion of
EAA
( Et BwihaihttaetbsseaysttatS(ecSeoxnutsrRaccEthsiyaocfena),Chaanmdl a!@wchaittfe eish (1csalorre ypmctates),
cc: hR0.eninitckeerr-206- . EE)
006290
Teas. River Fish/JEG
Pr.
5/22/79
INTRODUCTION IfSIiotltnuamoineraisoct,ckhne(fo3mlw)iu(co4art)lih.sdaetcaW3lni4et'vhseblistoD,hieeccsa1cet0uu.src5u,olmpabptAiaelnaeb(idna1)m(aff3ai)csp.thloaraistnI,taethfuhflselaubenaonelrtxsatotohrabyesenehaisvghhioewn that PIl0a,s8t98haLdf dfeitsehctcaabulgeht leinveltsheofTenfnleuscsreoecheRmiivcearlsn.ess the Desctaetpurwas BRESUELTSSaANDUDDpiLrssccuTussssiSton ohTfaibclihenthe1arevlseitstths(eP4ts3ha0em3e,cornecteFenNtn-rt39ai3to3in,ont,ianmdein Faspup-m3t,4he33i)nt.hrfeieshfolfuorcoocmhpoomuindcsals SagAanradmeapalltfyeeaetri.s34e,oxftItesnhttochwaesninratelhsteauhtoletDsgtehaesfstoerfreolniutnohtterheoasdcttihisentsmaheilecctameltudsrsacccbtlih,ecacsolrsnaeeycpeerrcsoatdtwiufeacisrtssihv,etosyaetem, rtoesbuiltosaccagurmeielawtieththeeartlhireere rfelpuoorrtosche(a3i4ca)l.s of interest, FTeeuiscsesor Stih4hnae0cnnewtchbwoooilatcephearfiftiihnssehghsftwahlmoeuprolerteooscct,ahauelgathihftcelauloilnracorocgtnhheteeremnscitach,maaelnn3e.Aclr7oe4nstc'epanpttmfsisvv(eehT.sFsCco)bonni1sfb3ooiirlp)actoh.emore f2$f33lodrpuloiarcnohnacacthneineoemnlmicoarfclooasrtffiwutsothhuti.lysdatbaiPeyvl'eubueoxeorpoecircuhetelpeaadrti.oecwdaolnsttobitionhaecichhuoimguhllaaprtagerertiitsiiaofnna:ttclyooersfs)feicciicent cft1hoi4aro3nn0nt9ohrleatohciwiasgtfhhpierasrevhs',ecnnootnstcaemabnptutlorohnait3giAdh,ooentrsotrchaccnainnnceoedbtnehterfroaorftisfoFaemnrupsedldedi.sn. theSmeindeciuespspheiicctoeeodsaicoummsilaotrTfhhiaegtofrltouwtuhooesrlofyccihosrehceaoismcsupaaammlrppselldees(stvwohhomiiwlyehcohlnfeoihtsahdfhivcasaovhleruesesrasmepptlmraeaeksyse.snbntefaTrthhoieimvgehrteehseseosmonnwciolnfltorramtottihaimnbuo is StihencerescuolrtesVcaamsplabye wroirgeoro(uuskloyn Icroommpartehdo. spproxiaste voecm' seireenclii"on, mDTTahaimeui.hlwwThwaFitsCaeptloiefbnat2s0i4s.c,4a0flhripodpumma.m4bpelwwlohhonoiw,lleoWhLetftehilwexeohruwTlhdFiDatCmeb,oefcdsri0aaf.mpf0ppi0lic4eeuip1ftpBr,mo:imnhasd"mhWaoiyvatehwtFhhaohonsoslseelteher OofltfoahlrelhgoreirwgihpneTogrIs:CsifbilluueodreuoxocphleoamnnilacyatliLoo'ncsotnhcefeonrwthritathteeiohbnia,gushedroLwiTnvPaiCntgiecaoInunldfthrevoempetthnoeecapiccyat.
006291
sewn iver masta BEST COPY AVAILABLE.
ommeemmcessseseuTcmRrTiIeN (0)
_--
ow Dam -- ERE
(1A - Whole fish
Tm-cm
0.40
om
{3a - core (5) 0.08
0.73
am
N.D. (8)
a1.o13 03
0.004 (4)
an,
.
Doaarss toaspmuosas ceasntoessveo. nrelsoiroedsdSishh GC aramters a(2) BSpEl Sor Salo 1d Baved on frozen weight of allan the fish. we, cviet,e,reprouativn
(or BcSREiLeAtdLhsLtcnv sa eevmuesegvensr ooff ssumamt wwSaenee,dcT 18e.88 Tion,co
006292
zee RET En) |
M CONFIDENTIAL
{
To wives mens BESTCOPY AVAILABLE 2/7
3 fa pn ue 5HH2eib5mhlincoBoR alrDelaSSsiaittipummsilareoedrE eBpem0raLfeeneeneacsntie]soeSoSftsisnhhheoote,tstu,eettncecoonnccatennssracion, R Tie etE e She rentL er of sh create gun 120 eorpnic
nox 3
ORGANIC (RF) AND INORGANIC (1)
FLUORIDE CONCENTRATIONS (ppm)
Sample
22
r
a
ws
1s
;
s F DErorape tynm in erisa h,yetlSsneieendeeir i hhe en aheTT a TAe, HSp SEC(iomEniEreREirrztTTeTsSRLhoEfttRiheeromaEntieitRTelsetaE BArhoEerhrT E etERradmeaCerlloirailtSse ccSiodoa:RnthteenaeLtsnhheeee
MR
006293
& feve Wels
;
NS
toma. naver rionsszc BESTGOPY AVAILABLE =/22/7
tic wiouldiseeeonnotoomfianthdei.cSaFetleuestrThoaactsheelntehirecalDcuomwn.hceenntTrhEaiotstiaosmnayoovfbeerfctlhhuaeosreoasxcahubym(1i)c,als
ee otohtaeSE TeTore the aaa"
C1FoEr8mopEaprerahaeoennoittreooafnuiSaoarmgapaFsonoiiryc2nceifdal.nu`pRoEirAeiA,dhspetovhseasSliouvhreiiesegthefssTrxtIopCmi,oiTraags5bai.ln0iei0osc4n
p1fplmua.aodrifd2oershtvohawelue, 16. ina there
FTe eonrenTH sEraatrlRiaoetresinatfe.osrcphLraeenasnieevnliteucsainlt.itylvsaenr,y| TBhihaeeahshcpeeocnicleeosnvetcsrhtaitcoihrognsahandiwhcihceh
te
LWTioithannelaeiem]oirttsecdtChweoomsoaicmooiptaullaaesetrospasotspipudelicasTtttoeierod:noonl(s{y3+ppeidfasiersfhdiinofooifftiebeoiencoleostnssccplsaeuomcsiisdsreoastnwe.saaaidnaysohFnaietver
Anemia
E1.XPERsIamMpElNeTAmLateriale Ha 34 = Spmraoltl rghuneged asatien e(Foteatursepudsssetusl, omght
1B - White Mertmre haar? bass (Rooous chiysops), caught below 'Wheeler 25 - White cR rappiR e Pomosis aneularis), caught shove Wheeler
TU et LI eee aP e T -- fumsares
P6309, YM-UULS, und PM-U1ZZ.
LTBoynedpitpemt tsithaeendaarTldaseooffiloF-S160a300t9p,apmnFLNt10-a"3a9d2a5r,dV,oarnuidmaeteFrtNih-ys3l42t2ahcawenoiert.oe.protpoamraerdk 3. Analysts Instrussntejuaterials
Blonder: TaErItNaSg rComomemraeisiBibloentdeer, eWtodeelo1f-3e6w3,Haarvtaeirlaa,bleCofarosmt.
006294
Te RI ror COPYAVAILABLE 7"
Toi var vesttnte from seks compa, Be 0. Bo
40,2,14 MaLo02 um aroseen sustiag ie as
mS--eee--msaaeod 5t.300s8,,asaisain fren Damn -- imnastsraimetsppobcyorlrioon. Cflma ppaeseeslsotto site ereesont:ata tate 713.05.
A ot 430 sone sta. rfromeBeeeiss-Fachare fn.
etn SstnomA78 oei . see ston steeelh, ches
hCCoolutrmon nTesTTpeemrraaRtucruers-a rIseosthpeogmnasl 18008 C.
A
Flow - ~40 cc/minute of Argon:Methane (95/5).
E
EEomLen areasSeRgrapy MC/B Manufacturing Chemists, sebvnt svatiabie from 29090 Nighland Avesue,
006295
(9) Bafanrsdoezdefnato.nweitghhet,actaunadlwweeiigghhttpeofrcesnatmploef eusuecdh, pa1r8t..8%. loss thun
Tenn. River Fish/JEG
=
5122/79
.
Hater:
Deloaized water.
3. Procedure (6) wPerroecedoubrteasineudsedfrboemloewa,rlieexrcepStM Tfeorchnmiicnaolr wRoedpiofrticastuimomnasr,ies (7). Saanmdplpelsac1e4d tinhroluagrhge3Aaluamnidou1sB wpeasrse, reinmo&vedfusferomhootdh,e afardeezer allowed to thaw. A22dwhohloemogcheansinzeeld ciantfaisbhl,endsearmplweith1A,30w0asBlcuwtateira.to 5 sections
Slaamtpelreal1Blihneadbeahidnidnketrhedigeillcoprleatsea.mpleCoattakeenntsjoufstthoeff the 3F0e.p5r0o1ducgtriavmesoarmgpalnes,werpaertskionf, kifdinleeyt,, samnadllrecptaurmt.of backbone,
SVgaeimrlptlelbeprlaa3etA,e.basdkiTnha,eda1inn6kd.e6rb3i4ldeig.erascoSrsaeammpplsleaesmpcl1oe8nttauanidkenne2d4bweIehirileentd, the homogenized with 10 ml of water ia & "tissuemizer."
Swoarmeplehom34ogewnaiszeddissweictthedw,ataenrd. theInvdeirviioduuasl ipnadritvsidwueailghpianrtgs mtohroesetohfal3e5s.s0ergwreaimgshtwewreerehohsoomgoegneizneidzedinina bale"ntdiesre,uewmhiizleer.' 2 Twaatbelre 3addleidstsforthehomsaomgpelnei,ziisgampelaechwesiagmhptl,e. and amount of Ad1i1viodefdthientaobofvievesaamlpilqeuso.tswfatnedrphloamcoegdeniinzaptrieocnl,easweedrebottlcs, T(hdriecehr1omiaqtueo/tawcidw,orywatpeirucerdinsiea, LdPrKy,ovtiovluwo,no,Whidrlye).ile oitnhearrtewforiwgeerreatoprlacated4.i5a%glCa.ssuntbiolttlneese.ded.Samples were stored
SpursepplaersedasncalcyozroddingfortoPt6h3e09,follFoHw-i3n9g35p,rocaenddurFsH.-42S2eeweTraoble 4 ffoorr `ewxetirgahcttioofnss.ample and milliliters of ethyl acetate used wAasprweeviigohuseldy(hmoomolgaerngiezredthasnamp4l.e0,0 sg)toraeadd aidadeadgltaoss& b3o0t-tmlle, parceectlaetaesveadsglaadsdsedcaetatrtihefugreatetuboef. 1.0A mvloleutmheylofsceethtyalte pur mgirxaemdoffohrom1o.g8enmaitoeu.tesThiea eathmyilxeraceattaate/sfpiesehd hsoemtotgienngatoef w3.ere The samples `were removed and centrifuged at 1500 rpm at
CCB296
vem wir rinse BESTCORY AVAILABLE
SLE reGe font BT Sa tfte 21 C. for 10 minutes. After centrifuging, the 2ethyl eESpnUit1eh, l34 SE 12e03p3Trm EeigniaR, isine S8ageerIr NBiEs
rb> nr F 2n0 smhI icLT tos sumeL. iAgR20 re sro ent) (3) Central Research Laboratory Report Number 6902, April 20, Bismemnie {PERI SLE (3) "Blocoucontration of FM-3423 in Dluogill Sunfish and in
(59 GBnaI, pAoe LaL vras E Roper ouR Rust P ATLSSe ;
(6 I bGS yiJmoamBieR lieE slea,rkML adyr7E s, 11927e0c. opl aysy3si)n B 4,3 eBter
4
BE DL AT NC el
isd Sagan
iG/cen
006297
son tee vie BESTCOPYAVAILABLE I _ vr S--
006298
Lean. River Pisn/smG -10-
5/22/19
TABLE 4
PISH WEIGHSTESDANYDORETEHXTYRLACATCIEOTNASTE VOLUMES
sume
W`eHiogmnotsenotftePish % iater EAFxcittsuhrnayNcet.ed ml
Description
Ckotma)~ Homogenste (mp). Eton
3% - all Bladder 1.20
s1.0 us2 12
an = Laver
2.20
62.7 s0.6 2.2
34 - uuscle
2.40
as ames 24
3h - rat
2.40
es. 20.2 24
31 - parts
3.00
4.3 asso 3.0
an - ais
3.00
s.8 ae.0 2.0
Water Bleak
2.40
100.0 -
2.4
1A
2.40
57.8
1012.8
2.4
Y
1B
2.40
32.7
1615.2
2.4
xn
2.00
ars 1s00.0 2.4
:
&==
-
oo
S
=
=
= g
=~=2
006299
|,
File Fate of FCB Cortns )pmine
.Fe3mamra 11a
TECHNICATLTRREEPPOORRTT SUMMARY rl5 e om12/28/79
TO: TECHNICAL COMMUNICATIONS CENTER -- 201.20N
1mpartant = reportsited on both sifdpapee,sndtwocopietso TCC.)
Environmental Laboratory (EI & PC)
ter Decatur, Alabama - Tennessee River Fish D. L. Bacon Fluorochemicals in Tennessee River Fish
0222
7c8r-o2n740
100
James E. Gagnon
51568 Lab Request #4871
seevewuOonnniorsv:hom amG00,ccoummonmeniTaoRECoTIivE:Ruorsnion Tden
Progress Report.
EE & PC Decatur
| "RCA
213531
3
Be a
a REPONT ABSToRrACT: (3R00R80ErdmTaos ia e errata' anna Tei Game Sor
The microwave sustained helium plasma detector
system and capillary column with electron capture
were utilized to examine fish extracts for fluoro-
carbon alcohol levels.
-
:
ce: DAl.WReilcckeerr-53-4
A.Mendel
006300
2
Introduction:
Pttrhoeegvriapopruhesysenwwicoterhk!ofanivneodllieacctatitrleoedn afclnauejoterudorcehfeomdriectmaeolcrset.ordeCf(aiCpGnCiiEltCli)avreyanadgnasmswiecrcrshorwotamovaesneteuhasyrtlai3na'ecsdetDahetecelaiteuuxnrt,rpalcaAtslsmaabaomfdaetfpiels>ahtnotrt.ak(eMAnSHMPfDir)nonmgwsetroheteauTbesrneondwenstsoebeualnlaRhilveyeasrde, saambpalcek,groeuxntdracchteecdk.as previously describedl, was also analyzed as
Results:
1. Capillary Gas Chromatography with Electron Capture
Nc(ohseammcipoclmaeplou1nsMd)tsanhdwaaevrridensg dr(eTetaetbceltneetdio1)ni.n ttihEmeexsceMpcitlnonsefesorottaao
bthreownflbuourlol-head peak at 6.14
bmInienluoctwoemspa,anrdiasanobdno,vseoslavWmehnpetleelsepres1kDBsa,ma,ndthre3eAspceh(crbtoaimsvsaetloaygn)rdasmchaowtwafesidshvmeorfryreomctlehaann.
d2e5tepceatkesd. inAsapmepalkeswit1hB arnedten3Atio(nTabtlieme1)s.imilar to F-6309 was
TABLE1 QUALITATIVE ANALYSIS OF FISH EXTRACTS FOR FLUOROCHEMICALS
sample w1mB F3ua-3422 Std. F-6309 Std. *N".D. == NVoetrydsemtaelcltedamount **##++ == P10eakppmAresatanldeasrsdthan
gRetpenteionpTTgiimmeei(i(oMginneunte-)s
N-.D.
NN.DD..
N*.D.
Nh.rD!
ie
N.D.
standard, but greater than *
2. Microwave Sustained Helium Plasma Detector: (MSHPD) mTainhcderosawubalovfveuerspaldmaepstlmeecastidowenetreecmotdoaerlss.oonaTsnhpaeilkyezrdeedsuslabtmysplMeoSsbHtPsaDhioniwendtthbhayet tfFhleMu-o3r4i2n2e eafxnltduroarFci-t6ns3e,09c,oNnotiefnftplrueoasrteoncttah,rebo0cn.o1ulpdpeapkmhsavlweeevrebeleenoibnsdeterhtveeecdettehdiynlftrhoaemcetatachtteueiarl Samples2.
006301
-3Discussion: TFheeSeantbovein rseasmuplltess.indicaThetelatrheaetapnoounvtoslaotfileorgfalnuoircocar]fluorine nmeonntvioolnaetdilein ftlhueoroocrhiegmiincaallsrep(oNrVtF:C).areThdiune-ltaoyetrhe cphrreosmeantcoegraofphy ifnorteNrVfFeCr'rsing(e.cgo.m,pouFnCd-s9.5) was hindered by an overabundance of Integrity of the Standards: FANf-t3e4r22thaereinbiottihalthreeposratm'e, coimtpwoausndbr(oNu-gehtthylupFOtShEatalFc-oh3o9l)2d3.;and rTheetenctoimopnountdimeu,sedby fgoarsoucrhroFmUa-t3o9g2r3apshtya,ndatrhdanhFaid-3g4i2v2e,n aSadmipflfeesrenotf FFMM--33992253 w(earenewsenstamptloe)C,ommFeMr-c3i9a2l3 C(htehmeicoallds"sAtnaanldyatridc"a)l, LFaMb-34f2o2r, and hvaedribfeiceantiiomnp.ropeIrtlywaslabdeelteedrmbienfeodrethbateintgheseonltd FtoM-u3s9.23 sIntanrdeaalridty, CthFe,,sSa0m,pNlHeELw)as. F-6309 (N-ethylperfluorooctanesulfonamide: Apsreopfara2t7ioAnuguosftst1a97n9d,ardtshe (niedwenFtMi-f3i9c2a3t,ionLotver51i8f,iedw"i)llabned uthseedolfdor FM-3923 has been properly labeled as F-6309. References: "oGfagnFolnu,orJoacmheesmicE.a,is34inTTeechnnniecsasleeReRpiovretr FSiusmhm,a"ry22"BMiaoyacc19u7m9u.lation 2DHeagteenr,minD.atiFo.,n o3fM TFelcuhonriicnaaltedRepAolrctohoSlusmmairnyFi"sAhR ENox.tra7c2t3s8,"-- 23 October 1979. personal Communication with A. Mendel. WNion.te1r4,598L,. 2D.4, AuCgoumsmterc1i9a79l. Chemicals Analytical Lab Request
JJeSue an bs
-v
006302
3M REPORT: BIOACCUMULATION STUDIES
March 8, 1993
(B1e2p1o2r8s17N9o),"F0l0u1or(o5c1h2e1m7i9ca)ls"BiinoaTcecnunmeuslsaeteiRoinveorfFFilshu.o*rochemicals in Tenn. River Fish and Report No. 100 rJperaroibitlyooeomnsf. cdBoeansitiregnontfs,pinaapeneldrasttiihsoenqduiitftofeioccuuoltntafyluolsfinaagnc,dtluaasrlagmedplelytiebnregmciapnuoasitneitoosn,fs.itnhceWorhrreeescnutltsthtaeadsdnedlasirte,leccootnmofbuoisuneredduinwddeienrhtistt3yanoJadfcikLn.agboefolsfriadenhnde:.
These papers make an excellent example of how a little knowledge can be dangerous.
6
006303
a
So
Heimerrina
TECHNICrAL REPORT SUMMARY
ecober 23, 1979
(Importan=t ifopor irieonbdoth siodfpaepesr,sendtwocopiesto TCC.)
CENTRAL RESEARCH LABORATORIES, Analytical and Properties Research Laboratory Environmental Engineering and Pollution Control
AR No. 7238 - Determination of Fluorinated Alcohols in Fish Extracts J. E. GAGNON - 209-15
0502 4000007 238
D. F. HAGEN - 201-15
042608.
FM T 0 oom X coe
wemoroonss.om sia Cunent omecTive
Tm Sotous
Request No. A73154
Central Research | Requestor - J. E. Gagnon
Jnalycical Repore| Project No. 91500600
BErcLHeEmcNa >
182 fNeiaComic tRapeness
Chentcal Analysis
SERSRMASBSTRCAoCTm:o(R1BH0w0eE5oT0ms Svfaoatnrnmraoinonound bYTemesCormancoon Gone 0 TchoelummnicwriocwhaveelescutsrtoaninecdapehuerlelundetpelcatsmLaon dweetreectourtilsiyzsetdem toandexacmaipniellary fish extracts for fluorocarbon alcohol levels.
norman Lon
errr
rem eeeem
= = =a = =<
=
==
==
~
006304
CENTRAL ANALYTICAL LABORATORY
Date. ___-- .__Octaher. 23, A979... BES TCOPY AVAILABI
Subject: Determination of Fluorinated Alcohols in Fish Extracts
Requestor: . J. E: Cagnon___
Dept. Name .BES?C_______ Proj. No, 1300600
Request No. .AZ313________
Report: Incroduceion
Dated . Au8g, 1u979st
Tahned mciacpriolvlaavrey csuosltuaminnecdhrhoemlaituongrapplhaysmawitdheteeclteocrtro(nMPcDa-pBt5u0r)e-cdhertoemcattioognrapvheirce ustyisltiezmesd Ft4o-3e9x2a5m,ineandfisFh-34e0x2t2r.act samples for the presence of fluorocarbon alcohols F-3923,
Discussion and Results
Tthhee hcehlrioumnatopglraasmpahicdetpeecatkorasyiietldeslutaetsomifcromlintehespceolcutmrna. forOnethecaneletmheenrtesforperemsoenntitoirn ssupcehcifasicaclhleyflfuororofclaurobroinnealacndohoslusl.furDetotecatliloonw floerveltsheadreeteicnttieornmeodfiatpeacbieftiwceencoFmIpDounds adnidctaEtCeddetbeycttohres.sampIlnetmhaitsritxy.pe oIff slaamrpglee,nont-hefluloorweirnedectoencttaiionningLinpietaksLsatseomepvrheastent otrheythewilqluarovtezrlcoaavditythetubpel.asmaThiasctipvraetsienngtsaLi"tbtylpeassdimfofdiec"ulttyo pirfevtehnetnocna-rfbolnuorbiunieldup {Innctoerrefste.renceThepealkosverarelevaedleqoufatetlhye sfelpuaorraotceadrbofnromalctoheholFsludoreitneectacbolnetaiinnintghespeeakethofyl acetate fish extracts ls about 5 nanograns/L0N1 injection.
Atdod2i0tuo1naalndseInnsjieccitviintgy1v0a4s1 oofbtatihniesdcboyncecnotnrcaetnetraftoringana1l0y0s4i1s.of Otpheerastoilnugtiocnonadsitiroencseived for the HPD-850 ace lisced below.
C1o0l0umcno 2S0v0stCenacA -1s6"'C,/at6n%.CHH-e2l0iH-uTnPAcaornrie8r0/a1c002m5eces/hmiCnh.rowmitG.h pHu.rPg.e prratoegrtaomedPDforfom50 cceh/rmoimna.togrFaoprthyanpderc6e0n%t tosftrthaenscfeorlruemd etoffltuheentMPDi-s85s0pilptlastmoathceaviFtIyD ovnia tahehHePa-t7e6d201/g1a6s" ciapislulsatraylouLdinebyaca 118000Cu.iceThmeicrcoauvaivtey hpooaudertseumpppelryatuorpeeraistinhegldatat2.25000Cgigaandhertehse, plasma TAhpeproexaitmsastieolny L0i.ne5snlu/saeidn.forof folxuyogrienne Lsandusesdulfasur tvheeresc6av8e5n6g.e0r anfdas 54t5o3.p9reAvenrtescpaercbtoinvely. buildup on the quartz plasna reactor tubes.
Tchaeptuarbeovedetseacmtpiloens vienreanalastotemepxtamitnoedloovnerathceapislelnasriytivciotlyumnlesveylsstemforwitthheecloemcptoruonnds of Ineresc. Operating conditions for the capillary system are listed below.
CCoolluumann Styesmtpeenrat8ur-e3v0asmet6e0rCgalnadss iccawpaisllaprryogcroalmummend waacll10cCo/aatiend.witcoh2C4H0-2C.0N. SplIintitimaolde of injectton was ucilized with 99% of the injected sample (ul) being vented to the
1
006305
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(ethyl acetate extract of a channel catfish above Wheeler Dam). At those points ethyl acetate extract of sample 1-B (bass below Wheeler Dam).
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006306
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SAcReoNos.ec 72233,8
Page 3
1979
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coPfh-r3oM4mI2a-2t3o9g2sr3paemmc.iaejso.rLoviTeshroemselerevbelulatsttoetfrhedtueiotseocmsteairmopnldeisvsitardoibeluhetacivteornoanfpsecnaaokpttautreevitdwheoenutlrdetienrnettqihueoinrescaimanpdeldeitional sample cleanup prior to chroaacography. Ttthhheeese0r.e1asluclpcopshmololsebvaeLilEnepidrnebstyehentteheccohyumllidcraochveaatvvaeetehepelenaxstmrdaaecttedsce.tteedcNtoofrrofaolnutoshrpeoiickraerdhlounsoarnmiepnalekesscwosenhrtoeewntochhasatterved In ehe actual samples.
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