Document 10dm54byx2gpjD4Vy5g1ELXzq
ARI6- 013
EE-/F
MICROBICS MICROTOX TOXICITY TEST
TEST SUBSTANCE
Identity:
Perfluorooctanesulfonate, Lithium salt; may also be referred to `as PFOS Li salt, FC-94, or FC-94-X. (1-Octanesulfonic acid, 1,1,2,2,3,34,4,55,6,6,7,7,8,8,8-heptadecafluoro-, lithium salt, CAS # 29457-72-5)
Remarks: The test sample is a mixtureof the test substance in water (approximately 24.5% test substance and 75.5% water). Nocalculations `were made to adjust for the actual concentration of the test substance in the test sample.
METHOD
--------------------
Method: Microbics Microtox "BASIC" Procedure
Test type: Static acute
GLP: No Year Completed: 1994 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition Statistical methods: EC10 and EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics, Reagent Lot # AMO25. Test conditions
Dilution water: Millipore Milli-QTM water Stock and test solutions preparation: A primary stock solution was prepared in dilution water at 2000 mg/L. The pHof the stock solution was then adjusted from 5.9 to 7.0 using 0.02N NaOH and an osmotic adjustment was made using 200 mg NaCl dissolved in 10 mL of stock solution. Appearance of test solutions was noted as "clear and colorless". All test solutions were made by proportional
dilution.
Exposure vessels: 3 mL glass cuvettes.
Number of replicates: 2 Number of concentrations: Four concentrations and controls Element basis: Percent light loss
B-- ERL ------------
Nominal concentrations: 125, 250, 500, 1000 mg/L
Element value:
15-minute EC1=o 564 (367-866) mg/L
15-minute ECso = >1000 mg/L.
30-minute EC = 487 (320-738) mg/L.
30-minute ECso = >1000 mg/L
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Remarks: Values reported are for the test sample. No calculations were made to adjust for the concentration of the test substance in the test sample.
CONCLUSIONS
`The test sample containing 24.5% Perfluorooctanesulfonate, Lithium salt exhibited a 30-minute ECsofor Photobacterium phosphoreumof >1000 mglL.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Kiimisch ranking 2. This study, while well conducted, lacks analytical data for: determination of the test substance concentration in the test solutions, and determinationof the sample purity.
REFERENCES
--
"This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number M1018, 2/07/24.
OTHER
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Last changed: 5/2/00
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ENVIRONMENTALLABORATORY MICROBICS MICROTOXTOXICITYTEST
La mote 80 254
SAMPLE: FC-94-X
Test Conc. mg/L)
125 250 500 1000 EC50, mglL (95% Cl)
PERCENT LIGHT LOSS OF P. phosporsum AFTER:
MinEpon
1880,Exons Exlpino. sure
[ 0 0 2 > 1000 )
7 5 9 15 > 1000 )
8 7 10 18 > I1z000
001544
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NIZROTOK DATA REFGRT
FILE NAME: M10128.K30
TTEESSTT DTAiTrEe:: -- _R-A=AM
SPIrnaovmceiesadtuirgDeae:tsocrrBsiApStIIiCoRn:D TiGrotox Testing Tnitiai Concentration 1000 ma/L
of
FGA-p9pG4ra-omXvoetdic ByAdjustments Dilution Factor : 2
Nacl
Tast TinTe:m30 minutes 10/17
conc. ConcentratsiaonntaUnits: me/L
2Uo 5w7.e75n, e73.s86 121255..00000000 ocloprrarces peecghe
I25 8GS9ea.il5zs4aa7ss 7r733a..n13s24 : 5153s 70.23
22S55000...00000000080 2320000
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7s 3931.17581,, seaa.laa33 11300060.00000050 poiaz0s22o18
CCOONRTRREOCLTIROAINTTI/OIFS0AC==TORB1=.2008../534407$646.69 83.78/5.87.58522%%
10 Z33 sDlDcDrDEBD=DI0D.D8D6D0D7DDDIDDDDIDEDODDDDDDDDDDDDDDD.DDEDDDDDDDDDDDDDID?33
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0203048.5767 (95% CONFIDENCE RANGE: 600.9686 10 %1S462.1895) 4 Used for calcslatians
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COEFFICIENT OF DETERMINATION: R2 = 0.71879
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xx `exTAPOLATED RESULTS
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MICROTIX DATA REPORT
FILE NAME: Mi018.K1S
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TTEESSTT DTAITMEE:: R=T]-qQY_
SIanmvpelsetigDaetsocrr:iptiSonT:DMicrotox Testing of FCf-p9o3r-oXved by:
PIrnoicteidaulreC:oncBeASnItCration : 1000 ma/L
ODsimlouttiiocnAdFjaucsttomren:t:2 Nasi
Test TipNeU:MEE1R5 minutes 10/17
conc. ConcentratsiaonaUnits: me/L
z1 9977..775477 7765..8009
3 Loa
slmas 72.73 5e.3T/ 74.58
= Ze.54/ 73.33
7& 5530..7553/7 6625..0836
CONTROL IeT/10 = 9B10.2.64/6/639.61.649
CORRECTIROANTIOFSACT=OR
=
0.8721 0.5351
112255..00050000 250.0000
520500..20000000
030000..00000000 8i.821/0009.70.0602%0%
2.8331%%
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EC503096.7422 (75% CONFIDENCE SANGE: 02.4584 TO %15917.8008) # Used for calculations
001549
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STATISTICAL REPORT FOR DATA FILE Mi018.k15
ESTINATING EQUATION: LOS = 0.7754 x LOG b +3.4905
NUMBER OF DATA PAIRS USED: &
|
RESIDUAL VARTANDE: 0.02548
95% CONFIDENCE FACTOR: 18018 FOR ECS0
CORRELATION COEFFICIENT: & = 0.04780
COEFFICIENT GF DETERMINATION: R2 = 0.71576
EFFECTIVE CONCENTRATIONS (95% CONFIDENCE RANGE):
EE5CC32100o =2=31'005986663.11775438270256 ammqao///LLL ((( 643086256...448925852422 TTTOOO %3821669579..18170i.7s)8r0a0xr8s) ECo=i 57.784 ma/L (13.5214 70 420.6260434 X53 EXTRAPOLATED RESULTS
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MICROTCX DATA REPORT
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FILE Nar: Mio1a.kS Investigator: TED
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Test TinNeo:reen3 minutes10/17
cone. ConcentratCairoinnaUnits: ma/L
32i aS97vr.i9l4s/ es85os.i7ss42
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CONTROLRAIT3TI/O1S0 2= s5i2z.e90r5./85e79l94i.e67
CORRECTION FACTOR = 0.8547
2212.5500.0000000600 15S2055o000a...l000o0000o600o00 | 3.9%1,050.083.879.06a04a04t0s
~~0o0..l000s1055300s832rxr --o0o0.ll.0o000z316a376v387ez7r% ol003ast
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001552
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HHH NUMBRERAH H CONS CENTA RH ATIONB AEGANA HARE
z:
121255..00000000 | 00..001094 %
3a
225500..00000000
00..008057 %%
s.
550000..00000000
-00..003i45 &
78
11000000..00000000
00..003033 *
* 7 INVALID DATA REMOVED FOR CALCULATION
1013.5 % EFFECT AH
10.15355
0.80
-9a.76r6
2.03
3eisz2
-2.67
35..2324
1.78
.
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001553