Document 10Nv7k4Y6KxapzygqemQDQxBq

AR ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE___________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-3017. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-3017. Current information indicates FC-3017 is a mixture of 0.67% PFOS, 20% diethylene glycol butyl ether, 55.41% water, 21% ethylene glycol, 1.33% Hydroxy foamer, 1% sodium octyl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, 0.04% sodium lauryl sulfate, and 0.05% tolyltraizole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect toxicity o f the fluorochemical component o f the test sample. METHOD:____________________________________________________ Method: OECD Guideline 203 Type: Acute static-renewal GLP: No Year completed: 1995 Species: Pimephales promelas Supplier: Aquatic Biosystems, Inc., Fort Collins, CO. Analytical monitoring: Temperature, pH, and DO. Exposure period: 96-hours Statistical methods: Not given. Test fish age: Juveniles (121 - 1 3 2 days) Length and weight: Average length = 23 mm Average weight = 0.0908 g (wet) Loading: 0.504 g/L Pretreatment: None Test conditions: Dilution water: Carbon-filtered well water Dilution water chemistry: Dissolved Oxygen: 8.4 mg/L pH: 8.4 Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis and mixed well with a glass rod. Concentrations dosing rate: Once for the control, 1.0 and 10 mg/L exposures. Twice for the 100 mg/L exposure, and three times for the 1000 mg/L exposure. Stability of the test chemical solutions: Not noted. Exposure vessels: 2.0 L beakers containing 1.8 L test solution. 002799 Number o f replicates: 1 for 1,10, and 100 mg/L exposures, control and 1000 mg/L exposure were duplicate. Number o f fish per replicate: 10. Number o f concentrations: four plus a blank control W ater chem istry during the study: pH range (0-96 hours): 8.0 - 8.4 (control exposure) 7.7 - 8.4 (1000 mg/L exposure) Temperature range (0-96 hours): 17.8 - 19.9 C Dissolved oxygen range (0-96 hours): 6.5 - 8.4 mg/L (control exposure) 0.7 - 8.4 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control, 1,10,100, and 1000 mg/L Element values: 96-hour LC50 = >1000 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS______________________________________________ The test sample 96-hour LC50 for fathead minnow was determined to be >1000 mg/L, the highest concentration tested. No mortality was observed at this concentration. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________ R eliability: Klimisch ranking = 3. Testing approach inconsistent among exposure concentrations, with a combination of static and static-renewal techniques used. Sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the> solution. REFERENCES_______________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number N3188, 1995. 002800 OTHER Last changed: 6/26/00 002801 i*i-r '.cc:?.s z .v.c Sample Description BEST COPY AVAILABLE Environmental Lab -- Work Sheet ^ - / / . //,,,/, t .. Las Request Ne. / V ?/' .V' Page / of A Date: _i / Analyst: A t y o l <>/ / T 'S / r c _________c____/__ ^ ^^ i ^ r 'C A u r< L f [ : k l ~L'< : U b j ? T 1 / - U s / d ffu . / / V t . - x V _-cfi-<e,vh Y.a^S Vo ! a*d is 7>t L <-r s " l t d /;> " l b /. Y A tA-^i TA h jlC',' ( omn iTioAJi?j r ii rl-iA/-, i . / f e r - e y i roee*l** ltoucoi /i(i??r. Mi ' *tu*g..J oOe // tv> t tI o-* r r, <! A/!- /.? i . la,'jL Cc.-j A o ( -J f f' rt C//, ru? s? Av /. Cfit ODD.. ? G (. f eft yu,..o(OQ^s u\g.r^'TV\^/0> plac~v? A im ic.L i"*eai<LeT~. .Ben. 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S Page -CDate: _ of H - Analyst: / sft*| ^ f CoasI 'Zr a. ' a*' c> c* r <_ /4 .j />n<x(id /- ^ / / *i*c/ /*'-?; /t s* a> cuv 3 f f *?i~! O <2<1 k 'Z**j L -7>e ,,/ / ~P~r^d'CdS S c ti>__- ^ .L'C-.rL ^..4-;__/< '<j ( c f * J /p 7^(C f r c- r fy b * <?<. 7 * r S L ?( a a ' s . i t r i -(_ (7<<-.' v\ r > > // ?' -.J? r yf. / & ^ a ) to. y ^ , , , , - / / . . ! -/ * 11 cl / / W U j f L o t f ^ X . j BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY 7 i-<, H LR N318B TYPE TEST: 96-HR ACUTE TOXICITY TO FATHEAD MINNOW, PIUEPHALES PROUELAS STATIC STATIC RENEWAL X TEST SUBSTANCE: FC-3017______________ __________________________________ DILUTION WATER: CARBON-FILTERED WELL WATER P M WELL >2. ST. PAUL, MN)__________ Tamp. 19.9 X .p H 8.4 . D.O. 8.4 rog/L TEST FISH SOURCE: AQUATIC BIOSYSTEMS, INC._______DATE RECEIVED: 7/26/1995 FORT COLLINS, CO. FISH AGE: 121-132days________ AVG.WT.: C.ViC ? CVjq AVG. SIZE: ? . 7 C ,)Cm NO. OFFISH/TESTCHAMBER: __________ 10 LOADING RATE: C . r-CM g / L TEST SOLUTION VOLUME: 1.8 L________ SIZE OF TEST VESSELS: 2.0 L BEAKER DATE INITIATED: 9/25/1995________ DATE TERMINATED: C! f 2 ` 1fa i 5 ~ TEST INITIAL TOTAL VESSEL CONC. AMOUNT 24-HR mg/L ADDED* BK1 CO NTRO L f' BK2 CONTROL C 1-A 1 1.8 PH .' s. s D.O. mA. vi 7 ,7 48-HR^ c MORTALITY pH D.O. 72-HR pH D.O. 96-HR pH D.O. mgA. 1 i mg/L i I mg/L y , i --o* ~ O ;y < C ' / C ! v i TTTO C ' * . ! ( : A 0 ri , 0 . 1 ' ' r ? i ' 'S. 7 1 1 I B 10 18 c ( i0 \ T. 1 (c '> ! ; ' ( I r s ~ 1 i i Y . z l C ( \\ 1- 1C00 180 a L c-.y o y .f r.,3 f ' ^ o C.1 r ` i S M \ C , L 1 !i 1-0 1000 1800 0 ? ?- G o c S'c 7 , 7 o 7 C 5e! 7,<=) / ! > 0 2 2- 1D000 1800 o 7 C 'c 7 . ?. C s'c! 5- -O i , i c S-.? i 2 . / TEMP. *C DATE 7 ANALYST 1 ^ - , LC50 (95% C.l.) .-y/ . . . ,. Ib'.O , t*-7 ci/2 C /ctS ~ G u ) J cn ? 7 a ?-t C i o s \ 1^(0C C a(~W /L >icco ' - K J l ', 9 t2 % h s 11, 3 iko rs. i f a fa < r g l~ s ^ M M E N T S .. INDIVIDUALWEIGHTS, mg/L. H= .? Tilt. ,ICCCrta.lL`.ai>^.: fa l u . L k I 's U flfVer Uft h<-S,'a0at72/l . `'iH C C A ^ k m A Q V C E C C ^ Y f i, O _ _ I a ! C ja iM lik # ! . >m I A, n . P m ^A Ia t I . _ L i ^ ^ . rnM i*J*.*^ ** IV1. V r* BEST COPY AVAILABLE ,,, Environmental Laboratory LR A.'3'fV Date ^2'1 96-Hour Acute Toxicity To Fathead Minnow, Plmephales Promelas Loading Rate Data Sheet Test Substance: I- C - _______________________________ Minnow # 1 2 3 4 5 6 7 8 9 10 Mean Avq. Weight, (g) C.I23' 0 IlS O 0 'o C C. 0 - 0 19C0 C -<f^ o or<z T o c . r i s~ C ,C `iC. i 0 . 0~7a 3 C. o8o$ io Lenqth, (cm) 7.6 7 .U 7 .2 . 7. , _ > ? ,rs 7u > -v -r 27, r c 1 Loacttng.Fl3te_CalfiulatI.Qii-i Mean Ava. wt..(a) 0.0*1 C3 x 1 0 = C .c|C2( q. in) a/t.OL = 0 7 tC '^ a /l.8 L (n) g= C- S o j Loadina Rate= C>. SOM a/L. . .. - 6 7 7 ** " cM V .* :> i r' *i > ,, -*i \