Document 10E8OzXJ3oL7k4EKLzv6pDk2Z

Date: July 15, 2024 Subject: From: Reconnaissance and Sampling Inspection Report Clean Water Act - National Pollutant Discharge Elimination System Worcester, MA Anna Hatke, Inspector ANNA Field Services Branch (FSB) Laboratory Services and Applied Science Division (LSASD) HATKE Digitally signed by ANNA HATKE Date: 2024.07.18 14:36:17 -04'00' Reviewed By: Jerome Keefe, Investigations Team Leader LSASD-FSB Digitally signed by JEROME JEROME KEEFE KEEFE Date: 2024.07.16 12:59:53 -04'00' To: Todd Borci, Manager Enforcement and Compliance Assurance Division (ECAD) Water Compliance Section 1 CC: Douglas Koopman, Inspector ECAD I. Facility Information A. Facility Name: City of Worcester, MA B. Facility Location: Worcester, MA C. Facility Contact: Jay J. Fink, Commissioner, Dept. of Public Works and Parks, dpw@worcesterma.gov, (508) 929-1300 D. NPDES Permit ID:MAS010002 (MS4) II. Background Information A. Date/Start time of Reconnaissance inspection: 05/16/2024 11:02 B. Weather conditions: Rainy, approx. 57F C. USEPA Representatives: Anna Hatke, Michelle Coombs D. State/Local Representatives: N/A A. Date/Start time of sampling inspection: 05/028/2024 09:45 B. Weather conditions: Sunny, approx. 70F Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection C. USEPA Representatives: Anna Hatke, Michelle Coombs D. State/Local Representatives: N/A E. Federally Enforceable Requirements Investigated: Stormwater discharges and certain non-stormwater discharges from the Worcester, MA Municipal Separate Storm Sewer Systems (MS4) are authorized by the National Pollutant Discharge Elimination System (NPDES) General Permit for the Discharge of Stormwater from Small MS4 (hereinafter, the Permit), issued by the Environmental Protection Agency (EPA) Region 1. The City obtained coverage under the Permit with NPDES Permit ID MAS010002. F. Pollutants Sampled: Pharmaceuticals and Personal Care Products (PPCPs), E. coli/Enterococcus, Ammonia (nitrogen), Total Chlorine, and Surfactants III. Disclaimer: Unless otherwise noted, this report describes conditions at the facility/property as observed by EPA inspector(s), and/or through records provided to and/or information reported to EPA inspector(s) by facility representatives and as understood by the inspector(s). This report may not capture all operations or activities ongoing at the time of the inspection. This report does not make final determinations on potential areas of concern. Nothing in this report affects EPA's authorities under federal statutes and regulations to pursue further investigation or action. IV. Type and Purpose of Inspection(s) The purpose of the reconnaissance inspection was to locate stormwater outfalls which discharge into Coal Mine Brook, O'Hara Brook, Fitzgerald Brook, and Lake Quinsigamond, while noting appearance, presence of flow, and access requirements for one or more sampling inspections. The purpose of the compliance sampling inspection was to identify potential illicit connections or illegal discharges within the Worcester, MA MS4 that may adversely impact the water quality in Coal Mine Brook, O'Hara Brook, Fitzgerald Brook, and Lake Quinsigamond. Samples were collected from 7 locations in accordance with the FSB Investigations Team Stormwater Program Plan. V. Permittee Details The City of Worcester occupies approximately 38.5 square miles in Worcester County, MA approximately 39 miles east of Boston, MA. The city shares borders with the municipalities of Holden and West Boylston to the north, Shrewsbury and North Grafton to the east, Millbury and Auburn to the south, Paxton, and Leicester to the west. According to the 2020 U.S. Census, the population of Worcester, MA was 206,518. VI. 05/16/2024 Reconnaissance Inspection Summary On May 16, 2024, Anna Hatke, Nafisah Ali, and Tori Macleod ("the EPA Inspection Team") conducted an unannounced reconnaissance inspection of the Worcester, MA MS4 at the locations described in Section VIII, including locations described under the map of the sample locations. They were not accompanied by any city or state representatives. The inspection started near 616 Page 2 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Plantation St, Worcester, MA at approximately 11:02. At the time of the inspection, the weather was rainy and approximately 57 F. According to the National Weather Service (NWS) for the Worcester Regional Airport (KORH) the last precipitation event prior to the inspection occurred on May 14, 2024, with 0.47 inches recorded. There was precipitation recorded within the 72 hours before the inspection of 0.02 inches on May 12, 2024. According to the NWS, in the month (to date) of May, the area observed 2.61 inches of rain, which is approximately 146% of the 30-year normal value of 1.78 inches. According to the US Drought Monitor, as of May 16, 2024, Worcester County is experiencing no drought conditions. VII. 05/28/2024 Sampling Inspection Summary On May 28, 2024, the EPA Inspection Team conducted an unannounced compliance sampling inspection of the Worcester, MA MS4 at the locations described in Section VIII. They were not accompanied by any city or state representatives. The inspection started near 616 Plantation St, Worcester, MA at approximately 9:45. At the time of the inspection, the weather was partly sunny and approximately 70 F. According to the National Weather Service (NWS) for the Worcester Regional Airport (KORH) the last precipitation event prior to the inspection occurred on 05/28/2024 with 0.00 inches recorded. There was precipitation recorded within the 72 hours before the inspection, on May 27, 2024, of 0.66 inches. According to the NWS, in the month (to date) of May, the area observed 7.01 inches of rain, which is approximately 220% of the 30-year normal value of 3.19 inches. According to the US Drought Monitor, as of May 28, 2024, Worcester County is experiencing no drought conditions. The sampling locations described in Section VIII were field screened using test kits for ammonia (nitrogen), surfactants, and total chlorine. In-situ measurements for specific conductivity, salinity, and temperature were also measured using a YSI water quality meter and recorded. Field data were recorded on field data sheets. Samples were delivered to and analyzed for E. coli, Enterococcus, and PPCPs at the EPA New England Regional Laboratory (NERL) in North Chelmsford, MA. Table 1 below summarizes the sampling and analytical data. VIII. Description of Sampling Locations (in order of collection time) Photographs depicting each sample location are included in Appendix A of this report. LQ-01: One of two circular concrete outfalls on either side of a large rectangular culvert, approximately 3 feet in diameter located at 616 Plantation Street. Discharge flow was approximately 20 gallons per minute ("gpm") into Coal Mine Brook. Sample was collected at the mouth of the right-side outfall, looking up-stream. A light sewage and musty odor was detected. Color was clear and turbidity was low. No floatables were observed. Dark green algae growth was observed on the inside of the concrete pipe. Trash was observed in the stream directly downstream of the outfall. Outfall sampled was chosen based on the Worcester, MA stormwater sewer system map. LQ-02: One circular concrete outfall located at the base of a stone wall beside a construction site, approximately 4 feet in diameter located at 382 Plantation Street. Page 3 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Discharge flow was approximately 5 gpm into an unnamed stream/brook. Sample was collected at the mouth of the outfall. A musty odor was detected. Color was clear and some particulate matter was observed. Foam clusters were observed coming from the mouth of the outfall. The outfall was three-quarters submerged. LQ-04BS: In-stream sample of Fitzgerald Brook discharging into Lake Quinsigamond collected at 240 Coburn Avenue. The sample was collected approximately 5 feet from the Northern bank of the stream at the mouth of a stone culvert outflow approximately 50 feet downstream of LQ-04B. A sewage odor was detected. Color was clear-brown, and turbidity was low. Foam was observed coming out of the culvert and collecting in an eddy directly downstream. Dark brown algae growth was observed on the rocks and stream bed directly downstream of the culvert. Trash and grass clippings were observed in and around the stream directly downstream of the culvert. LQ-04B: A singular circular clay outfall approximately 1 foot located on the inside of a concrete stormwater conveyance structure on the East side of a stone wall culvert at 240 Coburn Avenue. Discharge flow was approximately <1 gpm into Fitzgerald Brook. Sample was collected at the mouth of the outfall. A smaller pipe adjacent to the outflow was observed with no flow. A sweet earthy smell was detected. Color was clear and turbidity was low. No floatables were observed. Dark brown algae growth was observed on the inside of the outfall pipe as well as on the bottom of the concrete conveyance structure. Trash and grass clippings were observed upstream of the outfall. A duplicate sample was taken at this outfall location. LQ-05: One of two circular concrete outfalls, approximately 2 feet in diameter located at 5 Suntaug Road. Discharge flow was approximately 2 gpm into Lake Quinsigamond. Sample was collected at the mouth of the left-side outfall, looking up-stream. A slight brackish and rank odor was detected. Color was clear and turbidity was low. No floatables were observed. Green algae growth was observed on the inside of the concrete pipe as well as the flume leading directly into Lake Quinsigamond. Outfall sampled was chosen based on the Worcester, MA stormwater sewer system map. LQ-06BL: One of two circular concrete outfalls on either side of a larger circular concrete culvert, approximately 3 feet in diameter located at 10 Crane Street. Discharge flow was approximately 1 gpm into an unnamed stream discharging into Lake Quinsigamond. Sample was collected at the mouth of the left-side outfall, looking up-stream. No notable odor was detected. Color was clear and some suspended particulates were observed. Offwhite foam was observed coming from the outfall and collecting directly downstream of the outfall. LQ-06BR: One of two circular concrete outfalls on either side of a larger circular concrete culvert, approximately 1.5 feet in diameter located at 10 Crane Street. Discharge flow was approximately 5 gpm into an unnamed stream discharging into Lake Quinsigamond. Sample was collected at the mouth of the right-side outfall, looking up-stream. No notable odor was detected. Color was clear and some suspended particulates were observed. Foam was observed coming from the outfall and orange staining was observed on both the inside of the outfall pipe and on the rocks directly downstream of the outfall. Page 4 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Map of Sample Locations Note: the locations on the map were estimated using Google Earth and are used for display purposes only. LQ-01 LQ-04A LQ-04BS LQ-04B LQ-02 LQ-03 LQ-05 LQ-06BL LQ-06BR LQ-06A Note: LQ-03: No flow LQ-04A: No flow LQ-06A: Outfall was 80% submerged and no flow was observed/ water was stagnant Page 5 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Table 1: Summary of Sampling and Analytical Data Location Coordinates1 (decimal degrees) YSI Meter Field Test Kits (mg/L) Site ID Sample Date Sample Time North West Salinity, ppt. Temperature, C Conductivity, S/cm Ammonia Total Chlorine Surfactants LQ-01 05/28/24 9:59 42.2909 -71.7600 0.7 16.5 1363 0.20 0.10 0.40 LQ-02 05/28/24 10:42 42.2803 -71.7638 0.4 21.6 784.0 0.25 0.04 0.25 LQ-04BS 05/28/24 11:53 42.2640 -71.7592 0.2 15.0 428.3 0.25 0.03 0.25 LQ-04B 05/28/24 12:20 42.2641 -71.7592 0.1 13.1 177.3 0.25 0.02 0.25 LQ-05 05/28/24 13:00 42.2446 -71.7472 0.2 17.4 376.4 0.25 0.02 0.25 Bacteria (MPN/100mL) E. coli Enterococcus 2747 3466 9678 16 308 1674 1850 886 31 355 Cotinine Acetaminophen 9.1 4.0 7.3 5.6 2.1 530 3.7 260 ND 300 Paraxanthine 50 2.2 43 53 21 PPCPs (ng/L) Atenolol Caffeine ND ND ND ND ND 170 63 570 24 160 Metoprolol ND ND ND ND ND Diphenhydramine 2.5 ND 1.9 0.75 ND Carbamazepine 5.1 ND 0.77 ND ND Abbreviations and Notes: 1. The coordinates listed in Table 1 were derived from the map created in Google Earth, therefore the coordinates are estimates of the actual locations. N/A: Not Analyzed ND: Not Detected above Reporting Limit E. Coli/Fecal Coliform: Red 10,000 col/100ml, Orange 1,260 col/100ml, Yellow 235 col/100ml, Black < 235 col/100ml Entero: Red 1,000 col/100ml, Orange 350, Yellow 61 col/100ml, Black < 61 col/100ml NH3: Red 6 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L Cl2: Red 1.0 mg/L, Orange 0.3 mg/L, Yellow 0.02 mg/L, Black < 0.02 mg/L Surfactants: Red 1.0 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L (may give false positive at salinity greater than 1 ppt) PPCPs: Red 100x the RL,Orange 10x the RL,Yellow 3x the RL; Black < 3x the RL* *See Reporting Limit (RL) values for each compound in attached Laboratory Report for Source Tracking EPA notes while there are currently no numerical standards to compare pharmaceutical results against, it is EPA's experience that acetaminophen is the single best bacterial source tracking compound of those listed above, and any detection of this compound may indicate a source of sanitary sewage. With respect to all of the above compounds, when a sanitary sewage source is present, depending on the type of source, distance from the sample location, and the strength of the source, concentrations of these compounds may range from the low ng/l range up to thousands of ng/L. Page 6 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Table 1: Summary of Sampling and Analytical Data, cont. Location Coordinates1 (decimal degrees) YSI Meter Field Test Kits (mg/L) Bacteria (MPN/100mL) Site ID Sample Date Sample Time North West Salinity, ppt. Temperature, C Conductivity, S/cm Ammonia Total Chlorine Surfactants E. coli Enterococcus Cotinine Acetaminophen LQ-06BL 05/28/24 13:48 42.2387 -71.7506 0.1 14.2 211.8 0.15 0.00 0.25 39 31 ND ND LQ-06BR 05/28/24 13:50 42.2387 -71.7506 0.2 14.4 40.36 0.25 0.00 0.25 8 ND 1.4 ND Paraxanthine ND 2.9 PPCPs (ng/L) Atenolol Caffeine ND ND ND 14 Metoprolol ND ND Diphenhydramine ND ND Carbamazepine ND 0.41 Abbreviations and Notes: 1. The coordinates listed in Table 1 were derived from the map created in Google Earth, therefore the coordinates are estimates of the actual locations. N/A: Not Analyzed ND: Not Detected above Reporting Limit E. Coli/Fecal Coliform: Red 10,000 col/100ml, Orange 1,260 col/100ml, Yellow 235 col/100ml, Black < 235 col/100ml Entero: Red 1,000 col/100ml, Orange 350 col/100ml, Yellow 61 col/100ml, Black < 61 col/100ml NH3: Red 6 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L Cl2: Red 1.0 mg/L, Orange 0.5 mg/L, Yellow 0.02 mg/L, Black < 0.02 mg/L Surfactants: Red 1.0 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L (may give false positive at salinity greater than 1 ppt) PPCPs: Red 100x the RL, Orange 10x the RL, Yellow 3x the RL; No Pink < 3x the RL* *See Reporting Limit (RL) values for each compound in attached Laboratory Report for Source Tracking EPA notes while there are currently no numerical standards to compare pharmaceutical results against, it is EPA's experience that acetaminophen is the single best bacterial source tracking compound of those listed above, and any detection of this compound may indicate a source of sanitary sewage. With respect to all of the above compounds, when a sanitary sewage source is present, depending on the type of source, distance from the sample location, and the strength of the source, concentrations of these compounds may range from the low ng/l range up to thousands of ng/L. Page 7 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection IX. Appendix Appendix A: Photograph Log X. Attachments Attachment A: Laboratory Report for PPCPs (Source Tracking) Analysis Attachment B: Laboratory Report for E. coli Analysis Attachment C: Laboratory Report for Enterococcus Analysis End of Report Page 8 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Appendix A Photograph Log Page 9 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Photo 1: LQ-01 (photo taken 05/28/2024 at approx. 9:59) Stream located near 616 Plantation St, discharging into Coal Mine Brook, upstream of Lake Quinsigamond. Sample point is indicated by a red X. Page 10 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Photo 2: LQ-02 (photo taken 05/28/2024 at approx. 10:42) Outfall located near 382 Plantation Street, discharging into the unnamed stream uphill of Lake Quinsigamond. Sample points are indicated by a red X. Page 11 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Photo 3: LQ-04BS (photo taken 05/28/2024 at approx. 11:53) Stream located near 240 Coburn Ave, is Fitzgerald Brook which discharges into Lake Quinsigamond. In-stream Sample points are indicated by a red X. Page 12 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Photo 4: LQ-04B (photo taken 05/28/2024 at approx. 12:20) Outfall located near 240 Coburn Ave, discharging into Fitzgerald Brook which discharges into Lake Quinsigamond. Sample points are indicated by a red X. Page 13 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Photo 5: LQ-05 (photo taken 05/28/2024 at approx. 13:00) Outfall located near 5 Suntaug Road, discharging into Lake Quinsigamond. Sample points are indicated by a red X. Page 14 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Photo 6: LQ-06BL (photo taken 05/28/2024 at approx. 13:48) Outfall located near 10 Crane Street, discharging into an unnamed stream that discharges into Lake Quinsigamond. Sample points are indicated by a red X. Page 15 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Photo 7: LQ-06BR (photo taken 05/28/2024 at approx. 13:50) Outfall located near 10 Crane Street, discharging into the unnamed stream that discharges into Lake Quinsigamond. Sample points are indicated by a red X. Page 16 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Attachment A Laboratory Report for Source Tracking Analysis Author: LSASD Laboratory Services Branch Pages: 16 Page 17 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Attachment B Laboratory Report for E. coli Analysis Author: LSASD Laboratory Services Branch Pages: 4 Page 18 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Attachment C Laboratory Report for Enterococcus Analysis Author: LSASD Laboratory Services Branch Pages: 4 Page 19 of 20 Sampling Inspection Date: 05/28/2024 Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection End of Report Page 20 of 20 Sampling Inspection Date: 05/28/2024 June 25, 2024 Anna Hatke US EPA New England R1 United States Environmental Protection Agency Laboratory Services and Applied Science Division 11 Technology Drive North Chelmsford, MA 01863-2431 Microbiology Laboratory Report Project Number: 24050041 Project: Lake Quinsigamond Worcester, MA MS4 Analysis: E. Coli Defined Substrate Analyst: Jack Paar Date Samples Received by the Laboratory: 05/29/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log-in. Sample preparation and analysis was done following the EPA Region I SOP, TC/ECColilert4. Data were reviewed in accordance with the internal verification procedures described in the EPA New England LSASD Biology QA Plan. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8604. Sincerely, Digitally signed by JOHN PAAR Date: 2024.06.25 JOHN PAAR Jack A. Paar, III 13:41:38 -04'00' Biology Laboratory Manager Page 1 of 4 24050041ECMM Water Microbiology Laboratory Data Qualifier Codes J = Estimate H = Exceeds holding time I = Exceeds incubation time At = Atypical overgrowth S = Lost sample V = Insufficient sample volume TNTC = Too numerous to count MB = Media blank +++ = Positive control - - - = Negative control SP = Spiked Sample L = Estimated, result below reporting limit (RL) ND = Not Detected, result less than RL D = Lab Duplicate P = Plate counts outside preferred range FDUP = Field Duplicate collect time not specified on Chain of Custody. Field duplicate LIMS default time = 12:00AM Page 2 of 4 24050041ECMM Matrix: Stormwater UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION 1 - NEW ENGLAND LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 E. Coli Defined Substrate Sample Number LQ-01 LQ-02 LQ-04BS LQ-04B LQ-05 LQ-06BL LQ-06BR Lab ID AC13257 AC13258 AC13259 AC13260 AC13261 AC13262 AC13263 Date/Time of Collection 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 9:59 am 10:42 am 11:53 am 12:20 pm 1:00 pm 1:48 pm 1:50 pm Number of Samples: 7 Date/Time of Analysis 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 3:30 pm 3:30 pm 3:30 pm 3:30 pm 3:30 pm 3:30 pm 3:30 pm Compound E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate Concentration RL MPN/100 mL MPN/100 mLQualifier 2747 4 3466 4 9678 4 16 4 308 4 39 4 8 4 Page 3 of 4 24050041ECMM Page 4 of 4 June 25, 2024 Anna Hatke US EPA New England R1 United States Environmental Protection Agency Laboratory Services and Applied Science Division 11 Technology Drive North Chelmsford, MA 01863-2431 Microbiology Laboratory Report Project Number: 24050041 Project: Lake Quinsigamond Worcester, MA MS4 Analysis: Enterococcus in Water Analyst: Jack Paar Date Samples Received by the Laboratory: 05/29/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log-in. Sample preparation and analysis was done following the EPA Region I SOP, ECA Enterolert3. Data were reviewed in accordance with the internal verification procedures described in the EPA New England LSASD Biology QA Plan. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8604. Sincerely, Digitally signed by JOHN JOHN PAAR Date: 2024.06.25 13:44:26 PAAR Jack A. Paar, III -04'00' Biology Laboratory Manager Page 1 of 4 24050041ENTERO Water Microbiology Laboratory Data Qualifier Codes J = Estimate H = Exceeds holding time I = Exceeds incubation time At = Atypical overgrowth S = Lost sample V = Insufficient sample volume TNTC = Too numerous to count MB = Media blank +++ = Positive control - - - = Negative control SP = Spiked Sample L = Estimated, result below reporting limit (RL) ND = Not Detected, result less than RL D = Lab Duplicate P = Plate counts outside preferred range FDUP = Field Duplicate collect time not specified on Chain of Custody. Field duplicate LIMS default time = 12:00AM Page 2 of 4 24050041ENTERO Matrix: Stormwater UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION 1 - NEW ENGLAND LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 Enterococcus in Water Sample Number LQ-01 LQ-02 LQ-04BS LQ-04B LQ-05 LQ-06BL LQ-06BR Lab ID AC13257 AC13258 AC13259 AC13260 AC13261 AC13262 AC13263 Date/Time of Collection 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 9:59 am 10:42 am 11:53 am 12:20 pm 1:00 pm 1:48 pm 1:50 pm Number of Samples: 7 Date/Time of Analysis 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 05/28/24 3:30 pm 3:30 pm 3:30 pm 3:30 pm 3:30 pm 3:30 pm 3:30 pm Compound Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Concentration RL MPN/100mL MPN/100mL Qualifier 1674 10 1850 10 886 10 31 10 355 10 31 10 ND 10 Page 3 of 4 24050041ENTERO Page 4 of 4 June 12, 2024 Anna Hatke US EPA New England R1 United States Environmental Protection Agency Laboratory Services and Applied Science Division 11 Technology Drive North Chelmsford, MA 01863-2431 Laboratory Report Page 1 of 16 Project Number: 24050040 Project: Lake Quinsigamond Worcester, MA MS4 Analysis: HPLC/MS/MS Source Tracking Analysis EPA Chemist: Peter Philbrook Date Samples Received by the Laboratory: 05/29/2024 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log-in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP-LCMS_STA3. The SOP is based on an EPA Regional Analytical Method developed at the EPA New England Laboratory. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8340 . Sincerely, DANIEL Digitally signed by DANIEL BOUDREAU BOUDREAU Date: 2024.06.12 10:07:18 -04'00' 24050040$STA Qualifiers: RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value J1 = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E = Estimated value exceeds the calibration range L = Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P = The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC/MS. A = Suspected Aldol condensation product. N = Tentatively identified compound. Page 2 of 16 24050040$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 3 of 16 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ-01 5/28/2024 5/29/2024 6/04/2024 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 9.1 530 50 ND 170 ND 2.5 5.1 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AC13249 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 71 64 QC Ranges 37 - 113 8 - 114 Comments: 24050040$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 4 of 16 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ-02 5/28/2024 5/29/2024 6/04/2024 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 4.0 3.7 2.2 ND 63 ND ND ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AC13250 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 61 57 QC Ranges 37 - 113 8 - 114 Comments: 24050040$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 5 of 16 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ-04BS 5/28/2024 5/29/2024 6/04/2024 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 7.3 260 43 ND 570 ND 1.9 0.77 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AC13251 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 84 75 QC Ranges 37 - 113 8 - 114 Comments: 24050040$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 6 of 16 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ-04B 5/28/2024 5/29/2024 6/04/2024 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 5.6 ND 53 ND 24 ND 0.75 ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AC13252 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 78 71 QC Ranges 37 - 113 8 - 114 Comments: 24050040$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 7 of 16 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ-04B DUP 5/28/2024 5/29/2024 6/04/2024 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 5.8 ND 54 ND 26 ND 0.79 ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AC13253 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 76 74 QC Ranges 37 - 113 8 - 114 Comments: 24050040$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 8 of 16 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ-05 5/28/2024 5/29/2024 6/04/2024 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 2.1 300 21 ND 160 ND ND ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AC13254 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 66 66 QC Ranges 37 - 113 8 - 114 Comments: 24050040$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 9 of 16 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ-06BL 5/28/2024 5/29/2024 6/04/2024 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L ND ND ND ND ND ND ND ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AC13255 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 72 60 QC Ranges 37 - 113 8 - 114 Comments: 24050040$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 10 of 16 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ-06BR 5/28/2024 5/29/2024 6/04/2024 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 1.4 ND 2.9 ND 14 ND ND 0.41 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AC13256 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 75 65 QC Ranges 37 - 113 8 - 114 Comments: 24050040$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 Laboratory Blank Page 11 of 16 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: N/A N/A 5/29/2024 6/04/2024 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L ND ND ND ND ND ND ND ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: N/A Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 71 65 QC Ranges 37 - 113 8 - 114 Comments: 24050040$STA PARAMETER Acetaminophen Atenolol Caffeine Carbamazepine Cotinine Diphenhydramine Metoprolol Paraxanthine U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 MATRIX SPIKE (MS) RECOVERY Sample ID: AC13256 Page 12 of 16 SPIKE ADDED ng/L 120 120 240 24.0 24.0 24.0 120 120 SAMPLE CONCENTRATION ng/L ND ND 14.0 0.41 1.4 ND ND 2.9 MS MS CONCENTRATION % ng/L REC 95.1 79 106 88 208 81 23.8 98 20.0 78 17.6 73 105 88 109 88 QC LIMITS (% REC) 55 - 99 24 - 160 34 - 131 61 - 107 48 - 112 17 - 112 31 - 137 41 - 132 24050040$STA PARAMETER Acetaminophen Atenolol Caffeine Carbamazepine Cotinine Diphenhydramine Metoprolol Paraxanthine U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 Laboratory Duplicate Results Sample ID: AC13255 SAMPLE RESULT ng/L ND ND ND ND ND ND ND ND SAMPLE DUPLICATE RESULT ng/L ND ND ND ND ND ND ND ND PRECISION RPD % NC NC NC NC NC NC NC NC Page 13 of 16 QC LIMITS 50 50 50 50 50 50 50 50 24050040$STA PARAMETER Acetaminophen Atenolol Caffeine Carbamazepine Cotinine Diphenhydramine Metoprolol Paraxanthine Comments: U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Lake Quinsigamond Worcester, MA MS4 Laboratory Fortified Blank (LFB) Results LFB AMOUNT SPIKED ng/L 120 120 240 24 24 24 120 120 LFB RESULT ng/L 94.9 93.2 167 24.5 18.9 19.2 89.2 98.0 LFB RECOVERY % 79 78 70 102 79 80 74 82 Page 14 of 16 QC LIMITS % 45 - 106 33 - 129 41 - 120 63 - 108 55 - 106 15 - 113 41 - 122 52 - 115 Samples in Batch: AC13249, AC13250, AC13251, AC13252, AC13253, AC13254, AC13255, AC13256 24050040$STA Page 15 of 16 Page 16 of 16