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AR R26 - 0923 MICROBICS MICROTOX TOXICITY TEST TEST SUBSTANCE -- -- sm Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9i5u,oroorocatsanaescuolmfponoanteen,twohficFhX-m3a1y06a.ls(o1b-e Octanesulfonic acid) (CAS # 2795-39-3). RFeX-m3a1r0k6s,:PO#ThSe5233M3-p0r0o4d,ucEtAiIon85l5ot48n,umSbuemriwtaomso0315MG.JapTahne. teCsutrrseanmtple is ibuntfyolrmeatthieorn, i8n3d.i3ca8t%eswattiesr,a 1mi0x%tuertehoyfl2en.e3g1l%ycoPlF,O1S.,362%.6S%uldtioentehyfloeanmeergl,yc0o.l2% bexnaznotthrainazgoulme,.0.1% 2-biphenylol, sodium salt, tetrahydrate, and 0.05% cThhaerafcotlelrowiiznegd scounmcmeantrryataipopnlsioefsitmopuarmiitxiteusr.eDwaitthaimnacyomnpoltetaeclcyurately reflect the toxicity of the fluorochemical componentofthe test sample. METHOD: _ peer -- Method: Microbics Microtox "BASIC" Procedure TGeLsPt:tyNpoe: Acute static YSepeacriCeosm:plPehotleodb:ac1t9e8r5ium phosphoreum AEnxaploystiucrael pmeorniiotdo:ri1n5g-:mipnHutaensd osmotic condition. Statistical methods: EC50 values calculated by statistical linear regression pTreosgtroarm,gaen.gi.s,mprsoobiutr.ce: Microbics' Reagent. Test coDnidliuteinotn:s:Millipore Mill-QTM water TSetsotckteamnpdetreastturseo:luNtiootnnoptreedp.aration: Not given. ENxupmobseurreofverespsleilcsa:te4s:mtLwcouvettes. Number Element of concentrations: basis: Percent light Four loss plus a blank control RESULTS Nominal concentrations: Blank control, 10, 100, 500, and 1000 mg/L Elementvalue: _5-minute 15minute ECs ECso = = >1000 >1000 mg/L mg/L. 003020 Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS "The test substance 15-minute ECso for Photobacterium phosphoreum was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331,St. Paul, MN, 55133 patAQuAUTY Reliability: Kieidm.isTchherraenkisinngo3c.haSrtauctdeyrilzaactkisodnoesfctrihpetitoenstasnudbsrtecaonrcdesopufritthy,e method and the follow study lacks analytical confirmationofthe amountof fluorochemical proportion in the solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C2282, 1985. HER -- _-- Last changed: 6/26/00. 005021 ENVIROMENTAL LABORKTORY BECKMAN"S MICROTOX SYSTEM HIcrotox RAW DATA IRC 228 TEST SUBSTANCE: FX-3i0b PO#S 5233-004 Test oate:___[0-28- 85 ANALIST: Sah TRE EAT 85542, Si misome 3m Tapan (lot dis sample coverte [cro eon mer| sLoe "ER Tory PINAL ASSAY CONCENTRATION | READING [5-WIN. | 15-uIl. |30-MIN 10) 1(5) (15: 1(30) Bl BLANK CONTROL 96 93 so a orLICATE s7 [81 |7 2 10 mall 24 Y 8 " Duplicate $3 [79 | 75 loo mgl@ 78 72 | 69 a Duplicate se 78 | 75 B4 Soo mal2 So 6b ba co Doplicate $7 71 Al as loco mL 50 | 37 | 35 os DoplicaXe. er |59 [sv 003622 . BECKMAN'S HICROTOX SYSTEM [3 C 22 jes nie MICROTOX DATA REDUCTION = 10-38-85 Fx-3l0b P0#S 5333-004 EAT 35548, suk ratioss = HBL Sumitomo 3M Japan (Lot 0156) ER FThe Sank Tatios for BI and Cl should agree with 0.02. _-- - BN SEHR ATES a BLNCRB1ABTK IRAOTISO C+ LCLBBCK RRATAIOTIO [--od |o 4 za1| NORMALIZED LIGHT Loss (vprecr) = ean Bo lank Bt atisxal Semple Reading GRRPLECoverre |ZoSoosme | "o.0D1Wqm.c bes2 10 my IL -0.026 -o0.c1a Boomal]g5o:a6 d [oe5.s00] | bs i cmgo LC] Sit Ed Brocemfae] 602.35738 | S0.X20689 | LIGHT LOSS (34) = --1E4x-- x 100 bom |= | = ERLEcoverTE Brooma | SHIN.Xa | TWN.1a 22 132.33 | BrocomQ4ei]3122% 1 32 | EFFECTIVE CONCENTRATIONS (EC8) MG/L ot CHIN. -- SOHTNA . rie a [O oo T gsr 3041) | aang (miz= 380 Fo wHooos Jubelosg | _ 002023