Document 0rvKaa4o9oJwg8n4gGzdkKoM

Monsanto Industrial Chemicals Co. St. Louis, Missouri Date: December 10, 1976 APPLIED SCIENCES REPORT BIOACCUMULATION OF CHLORODIPHENYLMETHANES IN RATS- PILOT STUDIES Report Type: Report Number: Job Number(s): Reported By: Special Study AC-76-SS-7 A3-000-760.21 - 1621196 0. Hicks, W. J. Litschgi, J. P. Mieure C. F. Call is R. E. Keller 0. Hicks . W. J. Litschgi G. J. Levinskas J. P. Mieure M.~W. Stevens Q. E. Thompson J. C. Weber B2SL TIB T2F T3E A2SC T2F A2SC T3E B2SK CONFIDENTIAL INFORMATION. This report must not be sent outside the company without approval. The recipient is accountable for its safekeeping and proper disposal. DSW 297720 STLCOPCB4067631 ANALYTICAL CHEMISTRY AC-76-SS-7 BIOACCUMULATION OF CHLORODIPHENYLMETHANES IN RATS- PILOT STUDIES OBJECTIVE To determine the level of bioaccumulation of selected chlorodiphenylmethanes in white rat tissues using liquid chromatography. SUMMARY . Pilot feeding studies using three chlorodiphenylmethanes (MCS-1558, MCS-1560 and MCS-1826) at three feeding levels (25, 100 and 500 ppm) were conducted for 14 days. Excised fat tissue composites from five rats were then analyzed for the products, CONCENTRATION FOUND IN 14 DAY SACRIFICE (PPM) Feed Level MCS-1558 MCS-1560 MCS-1826 25 0.5 -19 2 * * * * * * 0.5 100 3.2 4.0 1.9 500 5.9 34 9.1 MCS-1558 and MCS-1560 show an altered pattern in the rat tissues indicating selective concentration and/or depletion of some isomers. These data were used to establish appropriate feeding levels for the 30 day bioaccumulation/depletion study. Feeding levels of 50 and 500 ppm were selected with an 11/8/76 starting date. TEST METHODS 1, Extraction and Clean-up. Ten g. portions of fat were homogenized three times with hexane/sodium sulfate to extract the lipid, using a Polytron tissue homogenizer. Sodium sulfate was added to absorb moisture. An aliquot was then transferred onto a deactivated alumina adsorptoin column and eluted with a 10% (V/V) methylene chloride/hexane mixture. This mixture was concentrated to "0.5 ml and analyzed ____ by liquid chromatography (LC). ...................................... .......... 2. Liquid Chromatography Measurement. MCS-1558, 1560 and 1826 were separated from residual lipid by reverse phase liquid chromatography. A Waters Associates Liquid Chromatograph and a 4.0 mm ID X 30 cm uBondapak C,g column (Waters Cat No. 27324) was used for the analysis. Detection waby UV using a Schoeffel SF 770 set at 215 nm. The LC conditions were as follows: Eluent: 60 parts acetonitrile and 40 parts 0.01 M Flow Rate: 1.5 ml/min. Mode: isocratic HgPO^ DSW297721 STLCOPCB4067632 AC-76-SS-7 Page two Quantitation of residuai product in the fat tissue was achieved using external standards of known concentrations. A calibration curve of the sum of the peak heights vs. concentration was prepared. Details, including recovery data, are contained in Physical Chemistry Method No. 76-22. MCS-1809 and MCS-1775 can also be measured using this method with a modifica, tion in the eluent. /omc Monsanto Industrial Chemicals Co. Applied Sciences St. Louis, Mo. 12/76 - 0. Hicks, W. J. Litschgi, J. P. Mieure DSW 297722 STLCOPCB4067633 DSW 297724 STLCOPCB4067635 ^M S q to STLCOPCB4067636