Document 0qOLwEy0ZJD2RQBV3jODk4rkd

ALGICIDAL ACTIVITY TEST SUBSTANCE IdentityA:mixturceontaininpgerfluorooctanesulfownhaitceh,may alsobe referredto as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-203. Currentinformationindicatesitisa mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol,2.66 % Sultonefoamer, 3% sodium octylsulfate0,.1% sodium laurylsulfate,and 0.05% tolyltriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: Standard No. 22, Relatingto Swimming Pool Water Treatment Chemicals and/orProcesses (NationalSanitationFoundation) Type (testtype): Acute Static GLP: No Year study performed: 1974 Species: Chiorellpayrenoidosaand Phorinidiuminundatum Source: In-houseculturesmaintainedatIndustriaBlio-testLaboratories, Inc.,Northbrook,IL. Element basis: Cellpopulationscalculatedfrom hemocytometer counts, turbidimetrimceasurement. Exposure period: 14 days StatisticaMlethods: Not given. Analyticalmonitoring: Not given. Test Conditions: Algal NutrientMedium: Allen'snutrientmedium. Stock and testsolution preparation: Algalsolutionsprepared by blending algaewith 100 mL ofAllen'smedium usinga Waring Blender. One mL aliquotsofthe testsubstance were handled as 100% activeand dilutedwith distillweadter and added toAllen'smedium. One mL of adjustedcell suspension was added to each testsolutionf,ora finalvolume of 100 mL. Exposure vessels: Flaskscontainingfinalvolume of 100 mL. Number of replicates:1 Initiaclellloading:7.5 x 104 cells/mfLorChlorell1a2,.5x 104 cells/mLfor Phorrnidium Number ofconcentratiosnisx:plusa blankcontrfoolrChlorelfliav,pelusa blankcontroflorPhormidium. Lighting:Notgiven Subculture:Inoculatebdoftlessubculturteodfreshmedium afteorne day incubationw,ithfinalreadingsmade after14 days inoculationT.hree dilutions ofthe testsubstance were subcultured(1:5,1:10,and 1:100). Water Chemistry: Not given. RESULTS Nominal concentrations: Bk control,1:5,1:10,1:100,1:150,1:1000, and 1:10000 dilutiofnorChlorellapyrenoidosa study. Bk control,1:5,1:10,1:100, 1:1000, and 1:10000 dilutiofnorPhorinidiuminundatum study. Results of the Study with Chloreliapyrenoidosa Sample (dilution) Control 1:5 1:10 1:100 1:150 1:1000 1:10000 1:5 subculture 1:10 subculture -1:100 subculture Gross Appearance (Color) green none none none Slightgreen tint green green none none none Turbidity (absorbance) >2.00 <0.01 <0.01 <O.oi 0.03 >2.00 >2.00 <O.oi <0.01 <0.01 Results of the Study with Phorinidium inundatum Sample (dilution) Control 1:10 1:100 1:1000 1:10000 1:5 subculture 1:10 subculture 1:100 subculture Gross Appearance (Color) green none none none green tint green tint none none none Turbidity (absorbance) >2.00 <0.01 <0.01 <0.01 >2.00 >2.00 <0.01 <0.01 <O.oi Element values are based on dilutionosf nominal concentrations. Remarks:Testinwgas conductedon themixturaes describeidntheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS Algicidalratherthan algistatiactionofthe testsubstance was obtained. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 3. Study lacksdescriptioannd recordsof the method followed.The sample puritywas not propedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportion inthe solution. REFERENCES Test was conducted by IndustriaBlio-TestLaboratoriesI,nc.,of Northbrook, IL atthe requestofthe 3M Company, St.Paul,MN, 1974. OTHER Last changed: 6/27/00 a eLteo HICROBIOLOC.Y -'6tN DO@r. B 10 - T E S T 1810 FRONTAGE ROAD NO0R R HBROOK, ILLINOIS 60062 FDA RFAS. NO. 14-t4glb REPORT TO 3M COMPANY A LGIC IDA L A CT ivi-ix- ue' TWO SAMPLES LABELED "All AND 11Blf JANUARY 23, 1974 IBT NO. 633-04473 .@a&d4z@Bi10 T ES T JaAma&w**Pw- REPORT TO 3M COMPANY ALGICIDAL ACTIVITY OF TWO SAMPLES LABELED "AllAND "B" JANUARY 23, 1974 IBT No. 633-04473 I. Introduction At the request of 3M Company, tests were conducted to determine the algicidalactivityof two samples identifiedas "A" and "B". Two species of algae, Chlorella pyrenoidosa and Phormidium inundatum, were used in the eveluation. .fm&d4z:Bd10 T E ST laA&@ Yo= 2 II. Sumr-llary Two samples, identifiedas "A" and "B", were submitted on November 20, 1973, by 3M Company. - The algicidal test was initiatedon November 30, 1973, using two species of algae, Chlorella Pyrenoidosa Wisc. 2005, Phormidium inundatum Wisc. 1093. The test was conducted according to a method outlined and -prepared by the National Sanitation Foundation, Standard No. 22, Relating to Swimming Pool Water Treitment Chemicals and/or Processes. The turbidimetric determination conformed to the procedure. recommended by Fitzgerald.* A 1:100 dilutionof Sample "A" or "B", effectively controlled the proliferation of Chlorella sp. and Phormidium sp. for a 14-day period. Cidal rather than static action of the candidate 'algacideswas obtained. Fitzgerald, G. P. Bioassay for Algicidil versus Algistatic Chexnica-Is,Water and Sewage Works, 110-296 (1963) Respectfully submitted, INDUSTRIAL BIO-TEST LABORATORIES, INC. Report prepared by: Daniel Chan, M.S. Gr up Leader Report approved ]by: January 23, 1974 dp A. David Baskin, "-Ph.D. Section Head, Microbiology YeAdw,d B 10 T.15T 3 III.Procedure A. Preparation of Inoculum Two species of algae, Chlorella pyrenoidosa Wisc. 2005, and Phormidium inundatum Wisc. 1093, were used as the test organisms. Each organism was subcultured at weekly intervals in Allen's neutrientmedium for three weeks, prior to inceptionof the test. The suspensions of Chlorella and Phormidium @vere adjusted 6 6 to contain 7. 5 x 10 and 12.5 x 10 cells/ml respectively. They were prepared by shaking the algae growth in the flasks, to which was added 100 ml of Allen'.smedium and blenderized for one minute in a Waring Blender. Cell populationswere calculatedfrom hemocytometer counts. B. Preparation of Sample One ml aliquots of test samples handled as 1000/oactive were diluted in distilledwater and added to Allexi'smedium for a final concentration ranging from 1:5 to 1:10,000. These concentrations were achieved after the additionof I ml of the adjusted algae cell suspension for a finalvolume of'100ml. The numbers of Chlorella 4 and ]Phormidium cells used were 7. 5 x 10 and 12.5 x 104 respectively. Six control flasks, two per species, were also prepared. All inoculated bottleswere subcultured to fresh medium after one day incubation. Final readings for the presence of algal growth in all bottleswere made fourteen days after inoculation. B 10 T E S T IaJmd&va4 Pom 4 IV. Results The resultsofthegrowth examination and turbidimetricmeasure- ment of allflasksinoculatedwith Chlorellapyrenoidosa and Phormidium inundatum used for the algicidaltestsare presentedin Table I and Ilrespectively. S 10 T E S T IaJ"a&t"36-- 5 TABLE I Results of the Gross and Turbiclimetric Examination of Chlorella Pvrenoidosa Algicidal Test Sample "A" (dilution) -Gross Appearance (color) Turbidity (absorbance) 1:5 1:10 1:100 -1:150 1:1,000 1:10,000 subculture 1:10 subculture 1:100 subculture Control none none none slightgreen green tint none nonenone green < 0.01 < 0.01 <.O. 0 1 0.03 > 2. 00 > 2. 00 < 0. 01 < 0. 01 < 0.01 > 2.00 Sample "B" 1:5 1:10 1:100 1:150 1:1,000 1:10, 000 1:5 subculture 1:10 subculture 1:100 subculture Control none none none slightgreen green green none none none green tint < 0.01 < 0.01 < 0.01 0.03 > 2.00 > 2.00 < 0.01 < 0.01 < 0.01 > 2.00 B 10 T E S T la4maA"" Pom TABLE.U Results o,fthe-.Grossand Turbidi,metric Examination of Phorinidium inandatum Algicidal Test Sample "A" (dilution) Gross Appearance (color) Turbidity (Absorbance) tl:10 l@100 1:13,000 1:5 subculture 1:10 subculture 1:100 subculture Control none none none green none none none green tint tint < 0.01 < 0.01 < 0.01 2.00 > 2.00 < 0.01 < 0.01 < 0.01 > 2. 00 Sample 'tB't 1:5 1:10 1:100 1:1,000 1:10,000 1:5 subculture 1:10 subculture 1:100 subculture Control none none none green green none none none green tint tint < 0.01 < 0.01 < 0.01 > 2.00 > 2.00 0...01 < 0.01 < 0.01 > 2.00