Document 0mNGq1On2vwBLoGZQXOZQrgM

P-1 Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPCL/MS 11 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Field Blank #1 K1000911-001 P-2 Service Request: K1000911 Date Collected: 2/ 1/10 0948 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U ND u 0.31 J ND u ND u ND u ND u 0.12 J 0.34 J ND u 0.39 J ND u 0.40 J ND u MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2 1 4/10 2/8/10 20:54 105462 190362 1 2/ 4/10 2/8/10 20:54 105462 190362 1 2/ 4/10 2/9/10 06:46 105462 190362 1 2/4/10 2/9/10 06:46 105462 190362 1 2/ 4/10 2/8/10 20:54 105462 190362 1 2/ 4/10 2/8/10 20:54 105462 190362 1 2/ 4/10 2/8/10 20:54 105462 190362 1 2/ 4/10 2/9/10 06:46 105462 190362 1 2/ 4/10 2/9/10 06:46 105462 190362 1 2/ 4/10 2/9/10 06:46 105462 190362 1 2/ 4/10 2/8/10 20:54 105462 190362 1 2/ 4/10 2/8/10 20:54 105462 190362 1 2/ 4/10 2/8/10 20:54 105462 190362 1 2/ 4/10 2/9/10 06:46 105462 190362 1 2/ 4/10 2/8/10 20:54 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodium perfluoro-l-[l,2,3,4-13C4] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 103 81 90 100 83 67 56 77 80 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 20:54 2/9/10 06:46 2/8/10 20:54 2/8/10 20:54 2/9/10 06:46 2/8/10 20:54 2/8/10 20:54 2/8/10 20:54 2/9/10 06:46 Q Note Comments: Printed 2/23/10 13:35 \\Inflow2\Stariims\LimsReps\AnalyticalReport rpt Form 1A 12 SuperSet Reference: 10-0000n 5093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, incorporated (CDM) Baron & Budd P.C/95779-75319 Water Ellyson "Raw" K1000911-002 p.3 Service Request: K1000911 Date Collected: 2/1/10 1002 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 1.6 J 1.9 J ND u ND u ND u 2.7 J 9.3 4.2 J 4.1 J 12 6.8 4.7 J ND u MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 21:01 105462 190362 1 2/ 4/10 2/8/10 21:01 105462 190362 1 1 1 4/10 2/9/10 06:56 105462 190362 1 2/4/10 2/9/10 06:56 105462 190362 1 2/ 4/10 2/8/10 21:01 105462 190362 1 2/ 4/10 2/8/10 21:01 105462 190362 1 2/ 4/10 2/8/10 21:01 105462 190362 1 2/ 4/10 2/9/10 06:56 105462 190362 1 2/ 4/10 2/9/10 06:56 105462 190362 1 2/ 4/10 2/9/10 06:56 105462 190362 1 2/ 4/10 2/8/10 21:01 105462 190362 1 2/ 4/10 2/8/10 21:01 105462 190362 1 2/ 4/10 2/8/10 21:01 105462 190362 1 2/ 4/10 2/9/10 06:56 105462 190362 1 2/ 4/10 2/8/10 21:01 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[ 1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid S o d iu m p e r flu o r o -1 -[1,2,3,4 - 1 3C4] o c ta n e s u lfo n a te Sodium perfluoro-1-hexane[1802]sulfonate %Rec 95 79 104 100 82 59 50 74 82 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 21:01 2/9/10 06:56 2/8/10 21:01 2/8/10 21:01 2/9/10 06:56 2/8/10 21:01 2/8/10 21:01 2/8/10 21:01 2/9/10 06:56 Q Note Comments: Printed 2/23/10 13:35 \\lnilow2\Starlims\LimsReps\AnalyticalReportrpt Form 1A 13 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Ellyson "Treated" K1000911-003 P-4 Service Request: K1000911 Date Collected: 211/10 1015 Date Received: 21 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Add Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 1.6 J 1.6 J ND U ND U ND U 2.7 J 9.2 4.4 J 4.5 J 12 6.9 4.7 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 21:09 105462 190362 1 2/ 4/10 2/8/10 21:09 105462 190362 1 2 1 4/10 2/9/10 07:05 105462 190362 1 2/ 4/10 2/9/10 07:05 105462 190362 1 2/ 4/10 2/8/10 21:09 105462 190362 1 2/ 4/10 2/8/10 21:09 105462 190362 1 2/ 4/10 2/8/10 21:09 105462 190362 1 2/ 4/10 2/9/10 07:05 105462 190362 1 2/ 4/10 2/9/10 07:05 105462 190362 1 2/ 4/10 2/9/10 07:05 105462 190362 1 2/ 4/10 2/8/10 21:09 105462 190362 1 21 4/10 2/8/10 21:09 105462 190362 1 2/ 4/10 2/8/10 21:09 105462 190362 1 2/ 4/10 2/9/10 07:05 105462 190362 1 2/ 4/10 2/8/10 21:09 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic add Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoicacid Perfluoro-n-[ 1,2-13C2]dodecanoic Acid Perfluoro-n-[ 1,2-13C2]undecanoic Acid Sodium p e r flu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 90 83 93 100 82 61 49 * 71 81 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 21:09 2/9/10 07:05 2/8/10 21:09 2/8/10 21:09 2/9/10 07:05 2/8/10 21:09 2/8/10 21:09 2/8/10 21:09 2/9/10 07:05 Q Note Comments: Printed 2/23/10 13:35 \\Inflow2\Starlims\LimsReps\AnaIyticalReport.rpt Form 1A 14 Superset Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Davis "Raw" K 1000911-004 P-5 Service Request: K1000911 Date Collected: 2/1/10 1040 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q 1.0 J ND U 0.91 J 0.89 J 0.23 J ND U ND U 1.4 J 3.0 J 2.1 J 0.36 J 4.1 J 4.0 J 2.4 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 21:16 105462 190362 1 2/ 4/10 2/8/10 21:16 105462 190362 1 2/ 4/10 2/9/10 07:15 105462 190362 1 2/ 4/10 2/9/10 07:15 105462 190362 1 2/ 4/10 2/8/10 21:16 105462 190362 1 2/ 4/10 2/8/10 21:16 105462 190362 1 2/ 4/10 2/8/10 21:16 105462 190362 1 2/ 4/10 2/9/10 07:15 105462 190362 1 2/ 4/10 2/9/10 07:15 105462 190362 1 2/ 4/10 2/9/10 07:15 105462 190362 1 2/4/10 2/8/10 21:16 105462 190362 1 2/ 4/10 2/8/10 21:16 105462 190362 1 2/ 4/10 2/8/10 21:16 105462 190362 1 2/ 4/10 2/9/10 07:15 105462 190362 1 2/ 4/10 2/8/10 21:16 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[ 1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodium p erflu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 96 82 106 100 81 55 51 70 84 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 21:16 2/9/10 07:15 2/8/10 21:16 2/8/10 21:16 2/9/10 07:15 2/8/10 21:16 2/8/10 21:16 2/8/10 21:16 2/9/10 07:15 Q Note Comments: Printed 2/23/10 13:35 \'Jnilow2\Starhms\LimsReps\AnaIytica}Report.rpt Form 1A 15 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Olive "Raw" K 1000911-006 P-6 Service Request: K 1000911 Date Collected: 2/ 1/10 1115 Date Received: 2/2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND u ND u 2.5 J 2.9 J ND u ND u ND u 4.4 J 9.6 7.3 1.9 J 9.1 10 7.9 ND u MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 21:31 105462 190362 1 2/4/10 2/8/10 21:31 105462 190362 1 2/ 4/10 2/9/10 07:34 105462 190362 1 2/ 4/10 2/9/10 07:34 105462 190362 1 2/ 4/10 2/8/10 21:31 105462 190362 1 2/ 4/10 2/8/10 21:31 105462 190362 1 2/ 4/10 2/8/10 21:31 105462 190362 1 2/ 4/10 2/9/10 07:34 105462 190362 1 2/ 4/10 2/9/10 07:34 105462 190362 1 2/ 4/10 2/9/10 07:34 105462 190362 1 2/ 4/10 2/8/10 21:31 105462 190362 1 2/ 4/10 2/8/10 21:31 105462 190362 1 2/ 4/10 2/8/10 21:31 105462 190362 1 2/ 4/10 2/9/10 07:34 105462 190362 1 2/ 4/10 2/8/10 21:31 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[ 1,2-13C2]undecanoic Acid Sodiumperfluoro-l-[l ,2,3,4-13C4] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 91 75 99 100 83 51 47 * 66 84 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 21:31 2/9/10 07:34 2/8/10 21:31 2/8/10 21:31 2/9/10 07:34 2/8/10 21:31 2/8/10 21:31 2/8/10 21:31 2/9/10 07:34 Q Note Comments: Printed 2/23/10 13:35 \Mnilow2\Starlims\LimsRepsVAnalyticalReport.rpt Form 1A 17 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Olive "Treated" K1000911-007 P-7 Service Request: K1000911 Date Collected: 2/ 1/10 1125 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND u 2.5 J 3.0 J ND u ND u ND u 4.5 J 9.6 7.3 1.8 J 9.5 9.6 8.1 ND u MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 21:39 105462 190362 1 2/ 4/10 2/8/10 21:39 105462 190362 1 2/4/10 2/9/10 07:43 105462 190362 1 2/ 4/10 2/9/10 07:43 105462 190362 1 2/ 4/10 2/8/10 21:39 105462 190362 1 2/ 4/10 2/8/10 21:39 105462 190362 1 2/ 4/10 2/8/10 21:39 105462 190362 1 2/ 4/10 2/9/10 07:43 105462 190362 1 2/ 4/10 2/9/10 07:43 105462 190362 1 2/ 4/10 2/9/10 07:43 105462 190362 1 2/ 4/10 2/8/10 21:39 105462 190362 1 2/ 4/10 2/8/10 21:39 105462 190362 1 2/ 4/10 2/8/10 21:39 105462 190362 1 2/ 4/10 2/9/10 07:43 105462 190362 1 2/ 4/10 2/8/10 21:39 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[ 1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodium p e r flu o r o -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 98 80 100 100 86 49 * 46 * 68 80 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 21:39 2/9/10 07:43 2/8/10 21:39 2/8/10 21:39 2/9/10 07:43 2/8/10 21:39 2/8/10 21:39 2/8/10 21:39 2/9/10 07:43 Q Note Comments: Printed 2/23/10 13:35 \\Inilow2\Starlims\LimsReps\AnalyticalReport.rpt Form 1A 18 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Carriage Hills "Raw" K1000911-008 p.8 Service Request: K1000911 Date Collected: 2/1/10 1156 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.32 J 0.36 J ND U ND U ND U 0.32 J 1.6 J 0.79 J ND U 1.6 J 1.3 J 0.84 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 21:46 105462 190362 1 2/ 4/10 2/8/10 21:46 105462 190362 1 2/ 4/10 2/9/10 07:53 105462 190362 1 2/ 4/10 2/9/10 07:53 105462 190362 1 2/ 4/10 2/8/10 21:46 105462 190362 1 2/ 4/10 2/8/10 21:46 105462 190362 1 2/ 4/10 2/8/10 21:46 105462 190362 1 2/ 4/10 2/9/10 07:53 105462 190362 1 2/ 4/10 2/9/10 07:53 105462 190362 1 2/ 4/10 2/9/10 07:53 105462 190362 1 2/ 4/10 2/8/10 21:46 105462 190362 1 2/ 4/10 2/8/10 21:46 105462 190362 1 2/ 4/10 2/8/10 21:46 105462 190362 1 2/ 4/10 2/9/10 07:53 105462 190362 1 2/ 4/10 2/8/10 21:46 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[ 1,2-13C2]undecanoic Acid Sodium p e r flu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 99 77 108 100 79 47 * 42 * 77 78 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 21:46 2/9/10 07:53 2/8/10 21:46 2/8/10 21:46 2/9/10 07:53 2/8/10 21:46 2/8/10 21:46 2/8/10 21:46 2/9/10 07:53 Q Note Comments: Printed 2/23/10 13:35 \\Inflow2\Starlims\LimsReps\AnalyticalReport.rpt Form 1A 19 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Carriage Hills "Treated" K1000911-009 P-9 Service Request: K1000911 Date Collected: 2/ 1/10 1206 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfiuorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.31 J 0.31 J 0.23 J ND U ND U 0.34 J 1.5 J 0.69 J ND U 1.4 J 1.2 J 0.76 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 21:54 105462 190362 1 2/ 4/10 2/8/10 21:54 105462 190362 1 2/ 4/10 2/9/10 08:03 105462 190362 1 2/4/10 2/9/10 08:03 105462 190362 1 2/4/10 2/8/10 21:54 105462 190362 1 2/ 4/10 2/8/10 21:54 105462 190362 1 2/4/10 2/8/10 21:54 105462 190362 1 2/4/10 2/9/10 08:03 105462 190362 1 2/ 4/10 2/9/10 08:03 105462 190362 1 2/ 4/10 2/9/10 08:03 105462 190362 1 2/ 4/10 2/8/10 21:54 105462 190362 1 2/ 4/10 2/8/10 21:54 105462 190362 1 2/ 4/10 2/8/10 21:54 105462 190362 1 2/ 4/10 2/9/10 08:03 105462 190362 1 2/ 4/10 2/8/10 21:54 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[ 1,2-13C2]dodecanoic Acid Perfluoro-n-[ 1,2-13C2]undecanoic Acid Sodium p e rflu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[ 1802]sulfonate %Rec 104 88 113 100 92 64 60 80 90 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 21:54 2/9/10 08:03 2/8/10 21:54 2/8/10 21:54 2/9/10 08:03 2/8/10 21:54 2/8/10 21:54 2/8/10 21:54 2/9/10 08:03 Q Note Comments: Printed 2/23/10 13:35 \\Inflow2\Starlims\LimsReps\AnalyticaReport.rpt Form 1A 20 Superset Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Watson "Raw" K 1000911-010 p. 10 Service Request: K1000911 Date Collected: 211/10 1225 Date Received: 2/2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.46 J 0.39 J 0.14 J ND U ND U 0.53 J 2.8 J 0.90 J 0.80 J 3.1 J 1.9 J 1.0 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 22:02 105462 190362 1 2/ 4/10 2/8/10 22:02 105462 190362 1 2/ 4/10 2/9/10 08:12 105462 190362 1 2/ 4/10 2/9/10 08:12 105462 190362 1 2/ 4/10 2/8/10 22:02 105462 190362 1 2/ 4/10 2/8/10 22:02 105462 190362 1 2/ 4/10 2/8/10 22:02 105462 190362 1 2/ 4/10 2/9/10 08:12 105462 190362 1 2/ 4/10 2/9/10 08:12 105462 190362 1 2/ 4/10 2/9/10 08:12 105462 190362 1 2/ 4/10 2/8/10 22:02 105462 190362 1 2 1 4/10 2/8/10 22:02 105462 190362 1 2/ 4/10 2/8/10 22:02 105462 190362 1 2/ 4/10 2/9/10 08:12 105462 190362 1 2/ 4/10 2/8/10 22:02 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoicacid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodium p e r flu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 103 89 111 100 94 51 48 * 77 93 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 22:02 2/9/10 08:12 2/8/10 22:02 2/8/10 22:02 2/9/10 08:12 2/8/10 22:02 2/8/10 22:02 2/8/10 22:02 2/9/10 08:12 Q Note Comments: Printed 2/23/10 13:35 \Mnflow2\Starlims\LimsReps\AnalyticalReport.rpt Form 1A SuperSet Reference: 10-0000135 093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Watson "Treated" K1000911-011 p. 11 Service Request: K1000911 Date Collected: 2/1/10 1235 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.32 J 0.51 J ND U ND U ND U 0.30 J 0.65 J 0.85 J 0.26 J 0.46 J 0.85 J 1.2 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 22:09 105462 190362 1 2/ 4/10 2/8/10 22:09 105462 190362 1 2/ 4/10 2/9/10 08:22 105462 190362 1 2/ 4/10 2/9/10 08:22 105462 190362 1 2/ 4/10 2/8/10 22:09 105462 190362 1 2/ 4/10 2/8/10 22:09 105462 190362 1 2/ 4/10 2/8/10 22:09 105462 190362 1 2/ 4/10 2/9/10 08:22 105462 190362 1 2/ 4/10 2/9/10 08:22 105462 190362 1 2/4/10 2/9/10 08:22 105462 190362 1 2/ 4/10 2/8/10 22:09 105462 190362 1 2/ 4/10 2/8/10 22:09 105462 190362 1 21 4/10 2/8/10 22:09 105462 190362 1 2/4/10 2/9/10 08:22 105462 190362 1 2/4/10 2/8/10 22:09 105462 190362 Surrogate Name Perfluoro-n-fl^^A.S-lSCS] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[l,2-13C2]undecanoic Acid Sodium p erflu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 107 85 111 100 91 66 56 77 87 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 22:09 2/9/10 08:22 2/8/10 22:09 2/8/10 22:09 2/9/10 08:22 2/8/10 22:09 2/8/10 22:09 2/8/10 22:09 2/9/10 08:22 Q Note Comments: Printed 2/23/10 13:35 \VInflow2\Starlims\LimsReps\AnalyticalReport.rpt Form 1A 22 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Kingsfield Road "Raw" K1000911-012 p. 12 Service Request: K1000911 Date Collected: 2/1/10 1340 Date Received: 2/2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q 0.88 J ND U 0.12 J 0.14 J 0.16 J ND U ND U ND U 0.17 J ND U ND U 0.22 J ND U 0.31 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 22:17 105462 190362 1 2/ 4/10 2/8/10 22:17 105462 190362 1 2/ 4/10 2/9/10 08:31 105462 190362 1 2/ 4/10 2/9/10 08:31 105462 190362 1 2/ 4/10 2/8/10 22:17 105462 190362 1 2/ 4/10 2/8/10 22:17 105462 190362 1 2/ 4/10 2/8/10 22:17 105462 190362 1 2/ 4/10 2/9/10 08:31 105462 190362 1 2/ 4/10 2/9/10 08:31 105462 190362 1 2/ 4/10 2/9/10 08:31 105462 190362 1 2/ 4/10 2/8/10 22:17 105462 190362 1 2/ 4/10 2/8/10 22:17 105462 190362 1 2/ 4/10 2/8/10 22:17 105462 190362 1 2/ 4/10 2/9/10 08:31 105462 190362 1 2/ 4/10 2/8/10 22:17 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodium perfluoro-l-[l,2,3,4-13C4] octanesulfonate Sodium perfluoro-1-hexane[ 1802]sulfonate %Rec 131 102 133 100 104 72 72 100 106 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 22:17 2/9/10 08:31 2/8/10 22:17 2/8/10 22:17 2/9/10 08:31 2/8/10 22:17 2/8/10 22:17 2/8/10 22:17 2/9/10 08:31 Q Note Comments: Printed 2/23/10 13:35 \\Inflow2\Starlims\LimsReps\AnalyticalReport.rpt Form 1A 23 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Kingsfield Road "Treated" K1000911-013 p. 13 Service Request: K1000911 Date Collected: 2/1/10 1355 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonatc Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.13 J 0.25 J ND U ND U ND U 0.28 J 0.26 J 0.60 J ND U 0.33 J ND U 0.96 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 22:24 105462 190362 1 2/ 4/10 2/8/10 22:24 105462 190362 1 2/ 4/10 2/9/10 08:41 105462 190362 1 2/ 4/10 2/9/10 08:41 105462 190362 1 2/ 4/10 2/8/10 22:24 105462 190362 1 2/ 4/10 2/8/10 22:24 105462 190362 1 2/4/10 2/8/10 22:24 105462 190362 1 2/4/10 2/9/10 08:41 105462 190362 1 2/ 4/10 2/9/10 08:41 105462 190362 1 2/ 4/10 2/9/10 08:41 105462 190362 1 2/4/10 2/8/10 22:24 105462 190362 1 2/ 4/10 2/8/10 22:24 105462 190362 1 2/ 4/10 2/8/10 22:24 105462 190362 1 2/ 4/10 2/9/10 08:41 105462 190362 1 2/ 4/10 2/8/10 22:24 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoicacid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[ 1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodium p e rflu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 76 57 84 100 66 49 * 45 * 67 90 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 22:24 2/9/10 08:41 2/8/10 22:24 2/8/10 22:24 2/9/10 08:41 2/8/10 22:24 2/8/10 22:24 2/8/10 22:24 2/9/10 08:41 Q Note Comments: Printed 2/23/10 13:35 \\Inflow2\Starlims\LimsReps\AnalyticalReport.rpt Form 1A 24 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Cantonment "Raw" K1000911-014 p. 14 Service Request: K 1000911 Date Collected: 2/1/10 1415 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.11 J 0.22 J ND u ND u ND u 0.29 J 0.16 J 0.48 J ND u 0.19 J ND u 0.28 J ND u MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 22:32 105462 190362 1 2/ 4/10 2/8/10 22:32 105462 190362 1 2/ 4/10 2/9/10 08:50 105462 190362 1 2/ 4/10 2/9/10 08:50 105462 190362 1 2/ 4/10 2/8/10 22:32 105462 190362 1 2/ 4/10 2/8/10 22:32 105462 190362 1 2/ 4/10 2/8/10 22:32 105462 190362 1 2/ 4/10 2/9/10 08:50 105462 190362 1 2/ 4/10 2/9/10 08:50 105462 190362 1 2/ 4/10 2/9/10 08:50 105462 190362 1 2/ 4/10 2/8/10 22:32 105462 190362 1 2/ 4/10 2/8/10 22:32 105462 190362 1 2/ 4/10 2/8/10 22:32 105462 190362 1 2/ 4/10 2/9/10 08:50 105462 190362 1 2/ 4/10 2/8/10 22:32 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[l,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sod ium p erflu o r o -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 81 46 * 75 100 51 52 49 * 77 80 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 22:32 2/9/10 08:50 2/8/10 22:32 2/8/10 22:32 2/9/10 08:50 2/8/10 22:32 2/8/10 22:32 2/8/10 22:32 2/9/10 08:50 Q Note Comments: Printed 2/23/10 13:35 \\Inilow2\Starhms\LimsReps\AnalyticalReport.rpt Form 1A Superset Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Cantonment "Treatment" K1000911-015 p. 15 Service Request: K1000911 Date Collected: 2/1/10 1426 Date Received: 2/2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutancsulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.12 J 0.29 J ND U ND U ND U 0.15 J 0.43 J 0.26 J ND U 0.35 J ND U 0.32 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 22:39 105462 190362 1 2/ 4/10 2/8/10 22:39 105462 190362 1 2/ 4/10 2/9/10 09:00 105462 190362 1 2/ 4/10 2/9/10 09:00 105462 190362 1 2/ 4/10 2/8/10 22:39 105462 190362 1 2/ 4/10 2/8/10 22:39 105462 190362 1 2/ 4/10 2/8/10 22:39 105462 190362 1 2/4/10 2/9/10 09:00 105462 190362 1 2/4/10 2/9/10 09:00 105462 190362 1 2/ 4/10 2/9/10 09:00 105462 190362 1 2/ 4/10 2/8/10 22:39 105462 190362 1 2/4/10 2/8/10 22:39 105462 190362 1 2/ 4/10 2/8/10 22:39 105462 190362 1 2/ 4/10 2/9/10 09:00 105462 190362 1 2 1 4/10 2/8/10 22:39 105462 190362 Surrogate Name Perfluoro-n-[I,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoicacid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[l,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodiumperfluoro-l-[l,2,3,4-13C4] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 80 61 84 100 74 43 * 44 * 67 86 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 22:39 2/9/10 09:00 2/8/10 22:39 2/8/10 22:39 2/9/10 09:00 2/8/10 22:39 2/8/10 22:39 2/8/10 22:39 2/9/10 09:00 Q Note Comments: Printed 2/23/10 13:35 \\Iniiow2\Starlims\LimsReps\AnalyticalReport.rpt Form 1A 26 Superset Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Nine Mile Road "Raw" K1000911-016 p. 16 Service Request: K1000911 Date Collected: 2/ 1/10 1457 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.24 J 0.34 J ND U ND U ND U 0.60 J 0.86 J 1.1 J ND U 0.57 J 8.1 0.85 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 22:47 105462 190362 1 2/ 4/10 2/8/10 22:47 105462 190362 1 2/ 4/10 2/9/10 09:09 105462 190362 1 2/ 4/10 2/9/10 09:09 105462 190362 1 2/ 4/10 2/8/10 22:47 105462 190362 1 2/ 4/10 2/8/10 22:47 105462 190362 1 2/ 4/10 2/8/10 22:47 105462 190362 1 2/ 4/10 2/9/10 09:09 105462 190362 1 2/ 4/10 2/9/10 09:09 105462 190362 1 2/ 4/10 2/9/10 09:09 105462 190362 1 2/ 4/10 2/8/10 22:47 105462 190362 1 2/ 4/10 2/8/10 22:47 105462 190362 1 2/ 4/10 2/8/10 22:47 105462 190362 1 2/ 4/10 2/9/10 09:09 105462 190362 1 2/ 4/10 2/8/10 22:47 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoicacid Perfluoro-n-[ 1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sod ium p e r flu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexanef 1802]sulfonate %Rec 73 40 * 62 100 46 * 53 52 77 80 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 22:47 2/9/10 09:09 2/8/10 22:47 2/8/10 22:47 2/9/10 09:09 2/8/10 22:47 2/8/10 22:47 2/8/10 22:47 2/9/10 09:09 Q Note Comments: Printed 2/23/10 13:35 \VInflow2\StarlimsVLimsReps\AnalyticalReport.rpt Form 1A 27 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Nine Mile Road "Treated" K1000911-017 p. 17 Service Request: K1000911 Date Collected: 2/1/10 1508 Date Received: 2/ 2/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.24 J 0.40 J 0.13 J ND U ND U 0.59 J 0.90 J 1.0 J ND U 0.76 J 7.8 0.59 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 22:55 105462 190362 1 2/ 4/10 2/8/10 22:55 105462 190362 1 2/ 4/10 2/9/10 09:19 105462 190362 1 2/ 4/10 2/9/10 09:19 105462 190362 1 2 1 4/10 2/8/10 22:55 105462 190362 1 2/ 4/10 2/8/10 22:55 105462 190362 1 2/ 4/10 2/8/10 22:55 105462 190362 1 2/ 4/10 2/9/10 09:19 105462 190362 1 2/ 4/10 2/9/10 09:19 105462 190362 1 2/ 4/10 2/9/10 09:19 105462 190362 1 2/ 4/10 2/8/10 22:55 105462 190362 1 2 1 4/10 2/8/10 22:55 105462 190362 1 2/ 4/10 2/8/10 22:55 105462 190362 1 2 1 4/10 2/9/10 09:19 105462 190362 1 2/ 4/10 2/8/10 22:55 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[ 1,2-13C2]dodecanoic Acid Perfluoro-n-[ 1,2-13C2]undecanoic Acid Sodium p erflu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 63 39 * 55 100 43 * 56 47 * 71 70 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 22:55 2/9/10 09:19 2/8/10 22:55 2/8/10 22:55 2/9/10 09:19 2/8/10 22:55 2/8/10 22:55 2/8/10 22:55 2/9/10 09:19 Q Note Comments: Printed 2/23/10 13:35 \\Inilow2\Starlims\LimsReps\AnalyticalReport.rpt Form 1A 28 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water No. 6 Well Plant "Raw" K 1000911-018 p. 18 Service Request: K1000911 Date Collected: 2/ 2/10 1404 Date Received: 2/ 3/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 1.2 J 1.6 J 0.27 J 0.60 J ND U 2.1 J 6.2 2.7 J 0.89 J 16 7.9 2.6 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 23:02 105462 190362 1 2/ 4/10 2/8/10 23:02 105462 190362 1 2/ 4/10 2/9/10 09:29 105462 190362 1 2/ 4/10 2/9/10 09:29 105462 190362 1 2/ 4/10 2/8/10 23:02 105462 190362 1 2/ 4/10 2/8/10 23:02 105462 190362 1 2/ 4/10 2/8/10 23:02 105462 190362 1 2/ 4/10 2/9/10 09:29 105462 190362 1 2/ 4/10 2/9/10 09:29 105462 190362 1 2/ 4/10 2/9/10 09:29 105462 190362 I 2/ 4/10 2/8/10 23:02 105462 190362 1 2/ 4/10 2/8/10 23:02 105462 190362 1 2/ 4/10 2/8/10 23:02 105462 190362 1 2/ 4/10 2/9/10 09:29 105462 190362 1 2/ 4/10 2/8/10 23:02 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[ 1,2-13C2]dodecanoic Acid Perfluoro-n-[l,2-13C2]undecanoic Acid S o d iu m p er flu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 82 69 96 100 79 51 43 * 67 87 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 23:02 2/9/10 09:29 2/8/10 23:02 2/8/10 23:02 2/9/10 09:29 2/8/10 23:02 2/8/10 23:02 2/8/10 23:02 2/9/10 09:29 Q Note Comments: Printed 2/23/10 13:35 \\Inflow2\Starlims\LimsReps\AnalyticalReport ipt Form 1A 29 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water No. 6 Well Plant "Treated" K 1000911-019 p. 19 Service Request: K 1000911 Date Collected: 2/2/101417 Date Received: 2/ 3/10 Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.18 J 1.2 J 0.12 J ND U ND U 0.25 J 0.35 J 0.72 J ND U 0.48 J 0.43 J 0.66 J ND U MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 23:10 105462 190362 1 2/ 4/10 2/8/10 23:10 105462 190362 1 2 1 4/10 2/9/10 09:38 105462 190362 1 2/ 4/10 2/9/10 09:38 105462 190362 1 2/ 4/10 2/8/10 23:10 105462 190362 1 2/ 4/10 2/8/10 23:10 105462 190362 1 2/ 4/10 2/8/10 23:10 105462 190362 1 2/ 4/10 2/9/10 09:38 105462 190362 1 2/ 4/10 2/9/10 09:38 105462 190362 1 2/ 4/10 2/9/10 09:38 105462 190362 1 2/ 4/10 2/8/10 23:10 105462 190362 1 2/ 4/10 2/8/10 23:10 105462 190362 1 2/ 4/10 2/8/10 23:10 105462 190362 1 2/ 4/10 2/9/10 09:38 105462 190362 1 2/ 4/10 2/8/10 23:10 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[ 1,2-13C2] decanoicacid Perfluoro-n-[l,2-13C2] hexanoicacid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodium perfluoro-l-[l,2,3,4-13C4] octanesulfonate Sodium perfluoro-1-hexane[ 1802jsulfonate %Rec 92 61 95 100 69 50 48 * 75 86 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 23:10 2/9/10 09:38 2/8/10 23:10 2/8/10 23:10 2/9/10 09:38 2/8/10 23:10 2/8/10 23:10 2/8/10 23:10 2/9/10 09:38 Q Note Comments: Printed 2/23/10 13:35 \\Inflow2\Starlims\LimsReps\AnalyticalReport.ipt Form 1A 30 SuperSet Reference: 10-0000135093 rev 00 ClientProject: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Method Blank KQ1000532-04 p. 20 Service Request: K1000911 Date Collected: NA Date Received: NA Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonatc Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q ND U ND U 0.13 J 0.27 J 0.22 J ND u ND u 0.097 J 0.21 J 0.27 J ND u 0.33 J ND u 0.60 J ND u MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 23:32 105462 190362 1 2/ 4/10 2/8/10 23:32 105462 190362 1 2/4/10 2/9/10 10:07 105462 190362 1 2/ 4/10 2/9/10 10:07 105462 190362 1 2/ 4/10 2/8/10 23:32 105462 190362 1 2/ 4/10 2/8/10 23:32 105462 190362 1 2/ 4/10 2/8/10 23:32 105462 190362 1 2/ 4/10 2/9/10 10:07 105462 190362 1 2/ 4/10 2/9/10 10:07 105462 190362 1 2/ 4/10 2/9/10 10:07 105462 190362 1 2/4/10 2/8/10 23:32 105462 190362 1 2/ 4/10 2/8/10 23:32 105462 190362 1 2/4/10 2/8/10 23:32 105462 190362 1 2/ 4/10 2/9/10 10:07 105462 190362 1 2/ 4/10 2/8/10 23:32 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoicacid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodium p e r flu o ro -l-[l,2 ,3 ,4 -1 3 C 4 ] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 93 81 94 100 82 65 53 76 80 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 23:32 2/9/10 10:07 2/8/10 23:32 2/8/10 23:32 2/9/10 10:07 2/8/10 23:32 2/8/10 23:32 2/8/10 23:32 2/9/10 10:07 Q Note Comments: Printed 2/23/10 13:35 \\InJflow2\StarUms\LimsReps\AnalyticalReport.rpt Form 1A 31 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Lab Control Sample KQ1000532-05 p. 21 Service Request: K1000911 Date Collected: NA Date Received: NA Units: ng/L Basis: NA Perfluorinated Sulfonic Adds and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q 104 79.5 90.5 98.5 60.0 86.0 102 98.5 92.5 95.0 95.5 98.0 95.0 98.5 106 MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 23:17 105462 190362 1 2/ 4/10 2/8/10 23:17 105462 190362 1 2/ 4/10 2/9/10 09:48 105462 190362 1 2/ 4/10 2/9/10 09:48 105462 190362 1 2/ 4/10 2/8/10 23:17 105462 190362 1 2/ 4/10 2/8/10 23:17 105462 190362 1 2/ 4/10 2/8/10 23:17 105462 190362 1 2/ 4/10 2/9/10 09:48 105462 190362 1 2/ 4/10 2/9/10 09:48 105462 190362 1 2/ 4/10 2/9/10 09:48 105462 190362 1 2/ 4/10 2/8/10 23:17 105462 190362 1 2/ 4/10 2/8/10 23:17 105462 190362 1 2 1 4/10 2/8/10 23:17 105462 190362 1 2/ 4/10 2/9/10 09:48 105462 190362 1 2/ 4/10 2/8/10 23:17 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoic acid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[l ,2-13C2]undecanoic Acid Sodium perfluoro-l-[l,2,3,4-13C4] octanesulfonate Sodium perfluoro-1-hexane[1802]sulfonate %Rec 96 82 90 100 85 64 53 76 83 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 23:17 2/9/10 09:48 2/8/10 23:17 2/8/10 23:17 2/9/10 09:48 2/8/10 23:17 2/8/10 23:17 2/8/10 23:17 2/9/10 09:48 Q Note Comments: Printed 2/23/10 13:35 \\Inilow2\Starl]ms\LimsReps\AnalyticajReport.rpt Form 1A 32 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: Sample Name: Lab Code: Analytical Report Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Lab Control Sample Dup KQ1000532-06 p. 22 Service Request: K1000911 Date Collected: NA Date Received: NA Units: ng/L Basis: NA Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonatc Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Result Q 99.5 94.0 94.5 97.0 61.0 85.5 108 92.0 95.5 96.5 88.0 98.0 102 100 93.0 MRL 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 MDL 0.46 0.95 0.029 0.11 0.095 0.48 0.85 0.081 0.030 0.11 0.12 0.046 0.066 0.11 1.3 Dilution Date Date Extraction Analysis Factor Extracted Analyzed Lot Lot Note 1 2/ 4/10 2/8/10 23:25 105462 190362 1 2/ 4/10 2/8/10 23:25 105462 190362 1 2/ 4/10 2/9/10 09:57 105462 190362 1 2/ 4/10 2/9/10 09:57 105462 190362 1 2/ 4/10 2/8/10 23:25 105462 190362 1 2/ 4/10 2/8/10 23:25 105462 190362 1 2/ 4/10 2/8/10 23:25 105462 190362 1 2/ 4/10 2/9/10 09:57 105462 190362 1 2/ 4/10 2/9/10 09:57 105462 190362 1 2/ 4/10 2/9/10 09:57 105462 190362 1 2/4/10 2/8/10 23:25 105462 190362 1 2/ 4/10 2/8/10 23:25 105462 190362 1 2/ 4/10 2/8/10 23:25 105462 190362 1 2/ 4/10 2/9/10 09:57 105462 190362 1 2/ 4/10 2/8/10 23:25 105462 190362 Surrogate Name Perfluoro-n-[l,2,3,4,5-13C5] nonanoic acid Perfluoro-n-[l,2,3,4-13C4] butanoic acid Perfluoro-n-[l,2,3,4-13C4] octanoic acid Perfluoro-n-[l,2-13C2] decanoic acid Perfluoro-n-[l,2-13C2] hexanoicacid Perfluoro-n-[1,2-13C2]dodecanoic Acid Perfluoro-n-[1,2-13C2]undecanoic Acid Sodiumperfluoro-l-[l,2,3,4-13C4] octanesulfonate Sodium perfluoro-1-hexane[ 1802]suIfonate %Rec 97 82 90 100 84 72 52 77 82 Control Limits 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 50-150 Date Analyzed 2/8/10 23:25 2/9/10 09:57 2/8/10 23:25 2/8/10 23:25 2/9/10 09:57 2/8/10 23:25 2/8/10 23:25 2/8/10 23:25 2/9/10 09:57 Q Note Comments: Printed 2/23/10 13:35 ' .Inflow2 .Starlims LimsReps'AnalyticalReport rpt Form 1A 33 SuperSet Reference: 10-0000135093 rev 00 Client: Project: Sample Matrix: QA/QC Report p. 23 Camp Dresser & McKee, Incorporated (CDM) Baron & Budd P.C/95779-75319 Water Service Request: K1000911 Date Analyzed: 2/ 8/10 - 2/ 9/10 Lab Control Sample Summary Perfluorinated Sulfonic Acids and Perfluorinated Carboxylic Acids by HPLC/MS Analytical Method: PFOA Prep Method: Method Units: ng/L Basis: NA Extraction Lot: 105462 Analyte Name Perfluoro-n-tetradecanoic acid Perfluoro-n-tridecanoic acid Perfluorobutanesulfonate Perfluorobutanoic Acid Perfluorodecane Sulfonate Perfluorodecanoic Acid Perfluorododecanoic Acid Perfluoroheptanoic Acid Perfluorohexanesulfonate Perfluorohexanoic Acid Perfluorononanoic Acid Perfluorooctanesulfonate Perfluorooctanoic Acid Perfluoropentanoic Acid Perfluoroundecanoic Acid Lab Control Sample KQ1000532-05 Result Expected % R 104 100 104 79.5 100 80 90.5 100 91 98.5 100 99 60.0 100 60 86.0 100 86 102 100 102 98.5 100 99 92.5 100 93 95.0 100 95 95.5 100 96 98.0 100 98 95.0 100 95 98.5 100 99 106 100 106 Duplicate Lab Control Sample KQ1000532-06 Result Expected % Ree % Ree Limits RPD RPD Limit 99.5 100 100 50 - 150 4 30 94.0 100 94 50 - 150 17 30 94.5 100 95 50 - 150 4 30 97.0 100 97 50 - 150 2 30 61.0 100 61 50 - 150 2 30 85.5 100 86 50 - 150 1 30 108 100 108 50 - 150 5 30 92.0 100 92 50 - 150 7 30 95.5 100 96 50-150 3 30 96.5 100 97 50 - 150 2 30 88.0 100 88 50 - 150 8 30 98.0 100 98 50 - 150 0 30 102 100 102 50 - 150 7 30 100 100 100 50 - 150 2 30 93.0 100 93 50 - 150 13 30 Comments: Printed 2/23/10 13:35 \\Inflow2\Starhms\LimsReps\LabControlSampie.rpt Lab Control Sample Summary 34 SuperSet Reference: 10-0000135093 rev 00 liimbia p. 24 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-001 2/8/2010 8:54:09 PM PFCklnetexLong.dam 41 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3080900 2753000 6362700 2512500 135020 6362700 148960 148960 148960 Analyte RT (Exp RT ) 0.00 (1.36) 0.00(2.42) 2.33 (2.53) 0.00 (3.34) 4.11 (4.06) 0.00 (4.10) 0.00(4.63) 0.00(5.14) 0.00 (5.74) Analyte Area 0 0 42921 0 5211 0 0 0 0 Calc. Cone (ng/mL) N/A N/A 0.078 N/A 0.019 N/A N/A N/A N/A Modified'' No No No No No No No No No Page 50 of 242 35 p. 25 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary : Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT) 0.00(1.36) 1.33(1.36) 2.27 (2.44) 2.26 (2.44) 2.34 (2.53) 2.31 (2.53) 0.00(3.34) 3.30(3.34) 0.00 (4.06) 4.10 (4.06) 4.25 (4.10) 4.77 (4.64) 4.75(4.64) 5.10(5.13) 5.44 (5.56) Area 0 3080900 494 2753000 8668 6362700 0 2512500 0 135020 736 2367 148960 141 192 Modified? Calc (Exp) MRM Ratio No 0.00 (2.38) Ratio Confirms ID No No 0.00(50.17) No No 0.00 (0.28) No No 0.00(19.17) No No 0.20(0.32) No No 148.24 (22.53) No No 0.00 (0.15) No No 0.00 (22.10) No No 0.00 (0.22) No No 25.91 (1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00 (0.36) No Page 51 of 242 36 /"C o Illffib iB Amsiyt/cal Services' Quantitative Peak Review 1.33 p. 26 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-001 PFOA 1 413.0/169.0 Da i_ Baseline d Missed n Wrnnn Peak PFOS 1 499.0/80.0 Da Baseline u Missed n Wrnnn Peak 4.11 PFUnA 1 563.0/519.0 Da g Baseline g Missed n Wrnnn Peak !____I I __ 13C-PFOA 417.0/372.0 Da Baseline Missed ___n Wrnnn P eak__ i PFNA 1 463.0/419.0 Da g Baseline Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline c Missed n Wrnnn Peak 4.10 PFDA 1 513.0/469.0 Da g Baseline Missed i Wrnnn Peak I 13C-PFUnA 565.0/270.0 Da Baseline Missed ` Wrnnn Peak l' A -A, a-/;' - i ''V PFDS 1 599.0/80.0 Da g Baseline u Missed n W rnnn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed __ n Wrnnn Peak___ _ ] 3.30 I 13C-PFDA 515.0/470.0 Da Baseline Missed i !Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak___ 4.75 J""i i ; ; hi, , l M:' ........It'.... T'-.... ......... Vf PFDoA 1 613.0/569.0 Da g Baseline Missed n Wrnnn Peak 13C-PFDoA 615.0/169.0 Da c Baseline o Missed n Wrnnn Peak .i': PFTrA 1 663.0/619.0 Da c Baseline a Missed n W rnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed ___n Wrnnn Peak___ ,,i PFTeA 1 713.0/669.0 Da c Baseline Missed r1W rnnn Peak 13C-PFDoA 615.0/169.0 Da d Baseline Missed n Wrnnn Peak Before After Page 52 of 242 37 Colum bia Arelytfcsi Services Ion Ratio Confirmation p. 27 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-001 Before After Page 53 of 242 38 p. 28 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 02081 OVSamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-001 2/9/2010 6:46:38 AM PFCkinetexShort.dam 41 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 13C-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 5132500 5831200 3272100 5831200 5831200 3272100 4639400 4639400 Analyte RT (Exp RT ) 1.25 (1.27) 3.60 (3.60) 0.00 (4.26) 5.32 (5.31) 6.25 (6.23) 6.33 (6.32) 6.33 (6.31) 7.68 (7.65) Analyte Area 64416 56244 0 76473 16038 33864 3272100 4639400 Calc. Cone (ng/mL) 0.06 0.08 N/A 0.07 0.01 0.02 0.80 1.00 Modified? No No Mo Mo No No No No V 1' 7 ^ 7 7 iC 7 -1 1> Page 49 of 245 39 ical p. 29 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary A n alyte Peak N am e PFBA2 PFPeA2 PFBS 2 PFHxA2 PFHpA2 PFHxS2 SJ3C-PFBA S_13C-PFHxA RT (Exp RT) 1.25(1.27) 3.60 (3.60) 0.00 (4.26) 5.32 (5.31) 6.24 (6.22) 6.34 (6.32) 1.26 (1.26) 5.31 (5.30) A rea 64416 56244 0 5399 155200 12701 5132500 5831200 Modified? Calc (Exp) MRM Ratio No 1.00(1.00) Ratio Confirms ID YES No 1.00(1.00) YES No 0.00(0.40) No No 0.07(0.10) YES No 9.68 (3.03) No No 0.38(0.40) YES No 79.68 (13.55) No No 76.25 (13.06) No Page 50 of 245 40 p. 30 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review T JL PFBA 1 213.0/169.0 Da a Baseline c Missed n Wrnnn Peak ;| | H II 13C-PFBA 217.0/172.0 Da a Baseline c_ Missed n Wrnnn Peak 6.^3 K1000911-001 G 5.31 ., 3.60 i ;:i ... Jl i 1 PFPeA 1 263.0/219.0 Da a Baseline l Missed n Wrnnn Peak xtrif .Mm 13C-PFHxA 315.0/270.0 Da c Baseline a Missed n Wrnnn Peak d '[ PFBS 1 299.0/80.0 Da c Baseline u Missed i :Wrnnn Peak ' 6^ 18 0 -P F H S 403.0/103.0 Da Baseline c Missed r Wrnnn Peak 5.31 PFHxA 1 313.0/269.0 Da G Baseline c Missed r Wrnnn Peak MM 6.^5 13C-PFHxA 315.0/270.0 Da Baseline Missed i , Wrnnn Peak 6,^3 PFHpA 1 363.0/169.0 Da u Baseline l Missed r= Wrnnn Peak 6.33 5l 13C-PFHxA 315.0/270.0 Da Baseline u Missed j i : Wrnnn Peak 7.^8 ' PFHxS 1 399.0/80.0 Da G Baseline Missed r, Wrnnn Peak 7.j>8 180-PFHS 403.0/103.0 Da Baseline u Missed n Wrnnn Peak | 7.68 i S 180-PFHS 403.0/103.0 Da Baseline u Missed i ; Wrnnn Peak_____ 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak S 13C-PFDA 13C-PFDA 515.0/470.0 Da 515.0/470.0 Da G Baseline g Missed Baseline u Missed r Wrnnn Peak_____ _____ h. Wrnnn Peak__ Before After Page 51 of 245 41 ervicss" p. 31 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation K1000911-001 .... ] PFBA2 213.0/169.0 Da l. Baseline c Missed i_ Wrong Peak 1 3.60 1 - L ........ PFPeA2 263.0/219.0 Da d Baseline u Missed c Wrong Peak PFBS 2 299.0/99.0 Da c Baseline Missed Wrong Peak ' -m a32"VJ'V WT ------ . PFHxA2 313.0/119.0 Da Baseline c Missed u Wrong Peak 6: I i. PFHpA2 363.0/319.0 Da u Baseline Missed l Wrong Peak PFHxS2 399.0/99.0 Da Baseline a Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed c Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 52 of 245 42 ft Columbia Analytical Services'" p. 32 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-002 2/8/2010 9:01:40 PM PFCkinetexLong.dam 42 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFD0A PFTeA 1 13C-PFD0A IS Area 2969100 2124500 5057300 2083500 98132 5057300 110130 110130 110130 Analyte RT (Exp RT ) 1.35 (1.36) 2.29 (2.42) 2.33 (2.53) 0.00 (3.34) 4.10 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00 (5.14) 0.00 (5.74) Analyte Area 191110 192670 1263000 0 0 0 0 0 Calc. Cone (ng/mL) 1.360 0.816 2.360 N/A N/A N/A N/A N/A Modified? No No No No No No No No No I Page 54 of 242 43 p. 33 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary A nalyte Peak Nam e PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.36 (1.36) 1.34(1.36) 2.28(2.44) 2.28 (2.44) 2.33(2.53) 2.34(2.53) 0.00 (3.34) 3.30 (3.34) 0.00 (4.06) 4.10 (4.06) 4.06(4.10) 4.59 (4.64) 4.75 (4.64) 4.90(5.13) 5.71 (5.56) A rea 370390 2969100 63342 2124500 443150 5057300 0 2083500 0 98132 418 1068 110130 565 502 M odified? No C alc (E xp) MRM R atio 1.94 (2.38) R atio C o n firm s ID YES No 15.54 (50.17) No No 0.33(0.28) YES No 11.03(19.17) No No 0.35(0.32) YES No 4.00 (22.53) No No 0.00 (0.15) No No 0 .0 0 (2 2 .1 0 ) No No 0 .0 0 (0 .2 2 ) No No 16.53(1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00(0.32) No No 0.00 (0.36) No Page 55 of 242 44 Quantitative Peak Review p. 34 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-002 l PFOA 1 413.0/169.0 Da Baseline Missed 11 W r o n n P e a k 13C -P F O A 417.0/372.0 Da Baseline Missed ___ n Wronn P e a k ____ 2.34 PFNA 1 463.0/419.0 Da u Baseline Missed 11 W rn n n P e a k 13C-PFNA 468.0/423.0 Da c Baseline Missed ___" Wronn Peak 3.30 PFOS 1 499.0/80.0 Da u Baseline d Missed ... i w'1 4,10 v PFUnA 1 563.0/519.0 Da c Baseline l Missed W rnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed T L__________ 13C-PFUnA 565.0/270.0 Da Baseline a Missed n W rnnn Peak PFDA 1 513.0/469.0 Da u Baseline Missed 11 Wrnnn Peak PFDS 1 599.0/80.0 Da Baseline u Missed n W rnnn P eak 13C-PFDA 515.0/470.0 Da Baseline o Missed n W rnnn P ea k 13C-PFOS 503.0/80.0 Da Baseline c Missed n Wronn Peak PFDoA 1 613.0/569.0 Da Baseline c Missed n W rnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n W rnnn Peak PFTrA 1 663.0/619.0 Da Baseline Missed ____ n W r n n n P e a k ____ 13C-PFDoA 615.0/169.0 Da g Baseline Missed ___n Wrnnn Peak PFTeA 1 713.0/669.0 Da u Baseline Missed ___r Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da u Baseline Missed ___i 1Wrong Peak___ Before After 45 Page 56 of 242 Ion Ratio Confirmation 1.36 p. 35 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-002 PFOA2 413.0/369.0 Da Baseline c Missed u Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da o Baseline Missed c Wrong Peak PFUnA2 563.0/269.0 Da c Baseline Missed Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed c Wrong Peak PFDoA2 613.0/169.0 Da Baseline u Missed g Wrong Peak PFTrA 2 663.0/169.0 Da a Baseline c Missed u Wrong Peak PFTeA2 713.0/169.0 Da g Baseline c Missed g Wrong Peak Before After Page 57 of 242 46 la :al S-mrnms p. 36 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-002 2/9/2010 6:56:13 AM PFCkinetexShort.dam 42 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 13C-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 4487100 5204800 3014900 5204800 5204800 3014900 4186200 4186200 Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.31 (5.31) 6.24 (6.23) 6.33 (6.32) 6.33 (6.31) 7.68 (7.65) Analyte Area 209530 480640 324200 496230 121740 1856300 3014900 4186200 Calc. Cone (ng/mL) 0.38 0.95 0.32 0.84 0.54 1.85 0.82 1.00 Modified? No No No No No No No No I i Page 53 of 245 47 jHLC o lu m b ia cL 3 Analytical Services ' p. 37 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary A nalyte Peak Nam e PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.26(1.27) 3.59(3.60) 4.25 (4.26) 5.31 (5.31) 6.24(6.22) 6.33 (6.32) 1.26 (1.26) 5.31 (5.30) A rea 209530 480640 119750 46794 383780 718720 4487100 5204800 M odified? No C alc (E xp) MRM R atio 1 .0 0 (1 .0 0 ) R atio C o n firm s ID YES No 1 .0 0 (1 .0 0 ) YES No 0.37(0.40) YES No 0.09(0.10) YES No 3.15(3.03) YES No 0.39(0.40) YES No 21.42(13.55) No No 10.49 (13.06) YES Page 54 of 245 48 p. 38 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review K1000911-002 i. PFBA 1 213.0/169.0 Da u Baseline i_ Missed i :Wrono Peak 4.25 ii 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak 6.^3 1I PFPeA 1 263.0/219.0 Da c Baseline Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline c Missed r. Wrnnn Peak ,'IL, ___J L . _____ - PFBS 1 299.0/80.0 Da c Baseline Missed i Wrnnn Peak 6.^1 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak 1I PFHxA 1 313.0/269.0 Da c Baseline Missed n Wrnnn Peak 6 33 iL 13C-PFHxA 315.0/270.0 Da c Baseline Missed 11 Wrnnn Peak ::;j 6.^3 | 1 PFHpA 1 363.0/169.0 Da l Baseline c Missed i Wrnnn Peak 6.33 13C-PFHXA 315.0/270.0 Da Baseline g Missed n Wrnnn Peak " ! 7.^8 i PFHxS 1 399.0/80.0 Da u Baseline u Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da u Baseline Missed n Wrnnn Peak ..4 7.68 L .... L _ 1 1^ [ S 180-PFHS 13C-PFDA S 13C-PFDA 403.0/103.0 Da 515.0/470.0 Da 515.0/470.0 Da G Baseline a Missed c Baseline Missed Baseline u Missed _____ r Wrnnn Peak_____ _____ n Wrnnn Peak_____ _____ n Wrnnn Peak_____ 13C-PFDA 515.0/470.0 Da u Baseline u Missed n Wrnnn Peak Before After Page 55 o f 245 49 Services- Ion Ratio Confirmation 1.26 3.59 p. 39 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000911-002 PFBA2 213.0/169.0 Da c Baseline Missed Wrong Peak 6,24 PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak I PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak . .I ___....... 6 33 ; 1 :........ a! :iVi- ........... PFHpA2 363.0/319.0 Da c Baseline Missed c. Wrong Peak PFHxS2 399.0/99.0 Da u Baseline Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed d Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da Baseline a Missed Wrong Peak Inismageisrdavaiable N/A Baseline Missed Wrong Peak Page 56 of 245 50 C ilim b ls * 3 A nalytical Services" p. 40 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-003 2/8/2010 9:09:13 PM PFCkinetexLong.dam 43 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFD0A IS Area 3030800 2314400 5596800 2400200 117240 5596800 125210 125210 125210 Analyte RT (Exp RT ) 1.35 (1.36) 2.28 (2.42) 2.34 (2.53) 0.00 (3.34) 4.10 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74) Analyte Area 197840 230630 1382700 0 3395 0 0 0 0 Calc. Cone (ng/mL) 1.380 0.894 2.330 N/A 0.009 N/A N/A N/A N/A Modified? No No No No No Mo No Mo No (0 /^ Page 58 of 242 51 JTC o lu m b ia Anaivtio! Services'- p. 41 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S 13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT) 1.36 (1.36) 1.34(1.36) 2.31 (2.44) 2.28 (2.44) 2.34 (2.53) 2.34(2.53) 0.00 (3.34) 3.31 (3.34) 0.00 (4.06) 4.12(4.06) 4.14(4.10) 4.75 (4.64) 4.75 (4.64) 5.35(5.13) 5.45 (5.56) Area 409930 3030800 68808 2314400 479440 5596800 0 2400200 0 117240 194 1535 125210 152 356 Modified? Calc (Exp) MRM Ratio No 2.07 (2.38) Ratio Confirms ID YES No 15.32 (50.17) No No 0.30(0.28) YES No 10.04(19.17) No No 0.35 (0.32) YES No 4.05(22.53) No No 0.00 (0.15) No No 0 .0 0 (2 2 .1 0 ) No No 0 .0 0 (0 .2 2 ) No No 34.53 (1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00 (0.36) No Page 59 of 242 52 lytical Quantitative Peak Review Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-003 PFOA 1 413.0/169.0 Da l Baseline u Missed r W ronn P eak ,i 13C-PFOA 417.0/372.0 Da Baseline Missed ___n Wrong Peak___ PFNA 1 463.0/419.0 Da c Baseline Missed n W rnnn P eak i 13C-PFNA 468.0/423.0 Da l, Baseline Missed ___n Wrnnn Peak___ 3.31 PFOS 1 499.0/80.0 Da c Baseline q Missed n W r n n n Peak .10 J 13C-PFOS 503.0/80.0 Da Baseline d Missed n W rnnn Peak 4.12 PFDA 1 513.0/469.0 Da a Baseline Missed n W rnnn Ppak 13C-PFDA 515.0/470.0 Da c Baseline Missed n W rnnn P eak PFUnA 1 563.0/519.0 Da l Baseline Missed r W rnnn P eak 13C-PFUnA 565.0/270.0 Da Baseline u Missed n W rnnn Peak PFDS 1 599.0/80.0 Da u Baseline c Missed n Wrnnn Peak___ 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak____ r PFDoA 1 613.0/569.0 Da Baseline Missed i - Wrnnn Peak 13C-PFDOA 615.0/169.0 Da u Baseline Missed h W rnnn Peak PFTrA 1 663.0/619.0 Da Baseline Missed ___r Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da c Baseline Missed r i W rnnn P pak PFTeA 1 713.0/669.0 Da L Baseline Missed ____ i W r n n n P e a k 13C-PFDOA 615.0/169.0 Da c. Baseline Missed r W rnnn P eak Before After 53 Page 60 of 242 Ion Ratio Confirmation 1.36 p. 43 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-003 PFOA2 413.0/369.0 Da Baseline c Missed Wrong Peak j PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak | PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak PFUnA 2 563.0/269.0 Da u Baseline Missed o Wrong Peak ;c '',JUll 15 JO J G PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak lj PFDoA2 613.0/169.0 Da l Baseline Missed Wrong Peak iJ PFTrA2 663.0/169.0 Da Baseline c Missed Wrong Peak su PFTeA2 713.0/169.0 Da Baseline Missed u Wrong Peak Before After Page 61 of 242 54 / T o iu m h ia O A nsitica! p. 44 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-003 2/9/2010 7:05:45 AM PFCkinetexShort.dam 43 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 4917200 5373200 3085300 5373200 5373200 3085300 4335800 4335800 Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.26 (4.26) 5.31 (5.31) 6.24 (6.23) 6.34 (6.32) 6.34 (6.31) 7.68 (7.65) Analyte Area 201100 494390 332750 533710 124120 1881000 3085300 4335800 Calc. Cone (ng/mL) 0.33 0.94 0.32 0.87 0.54 1.83 0.81 1.00 Modified? No No No No No No No No Page 57 of 245 55 / C o lu m b ia 4 * 3 Analytical Sorvico/r p. 45 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT) 1.26(1.27) 3.59(3.60) 4.26 (4.26) 5.32(5.31) 6.24 (6.22) 6.34 (6.32) 1.26 (1.26) 5.31 (5.30) Area 201100 494390 124860 49959 394700 744190 4917200 5373200 Modified? Calc (Exp) MRM Ratio No 1 .0 0 (1 .0 0 ) Ratio Confirms ID YES No 1 .0 0 (1 .0 0 ) YES No 0.38(0.40) YES No 0.09(0.10) YES No 3.18(3.03) YES No 0.40(0.40) YES No 24.45 (13.55) No No 10.07(13.06) No Page 58 of 245 56 itola :k-? S e . m c s s Quantitative Peak Review p. 46 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000911-003 PFBA 1 213.0/169.0 Da g Baseline e Missed i Wronn Peak ...I 13C-PFBA 217.0/172.0 Da Baseline c Missed n Wrong Peak 6. PFPeA 1 263.0/219.0 Da c Baseline u Missed n W rnnn P eak 13C-PFHxA 315.0/270.0 Da l Baseline Missed n Wronn Peak PFBS 1 299.0/80.0 Da c Baseline a Missed i ' Wrnnn Ponk 6,24 18 0 -P F H S 403.0/103.0 Da d Baseline c Missed n W rnnn Peak PFHxA 1 313.0/269.0 Da c Baseline Missed n Wrnnn Peak 6.34 13C-PFHxA 315.0/270.0 Da Baseline Missed n W rnnn P eak PFHpA 1 363.0/169.0 Da l Baseline u Missed ri W rn n n P e a k 6.34 13C-PFHXA 315.0/270.0 Da u Baseline c Missed n Wronn Peak PFHxS 1 399.0/80.0 Da g Baseline c Missed n W rnnn P eak 18 0 -P F H S 403.0/103.0 Da u Baseline Missed n Wrnnn Peak 7,68 S J 8 0 -P F H S 403.0/103.0 Da g Baseline Missed ____ n W r n n n P n a k 13C-PFDA 515.0/470.0 Da g Baseline Missed ___n Wrnnn Peak SJ3C-PFDA 515.0/470.0 Da u Baseline u Missed n W rn n n Peak___ Before After 13C-PFDA 515.0/470.0 Da d Baseline Missed n Wronn Peak Page 59 of 245 57 liimbia siytical Services" Created with Analfst^eporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 1.26 3.59 K1000911-003 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak 6.24 I PFPeA2 263.0/219.0 Da Baseline Missed c Wrong Peak 1 . 1. PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak |l lei 5, PFHxA2 313.0/119.0 Da Baseline g Missed Wrong Peak * A _______ 6. 54____;____ A w .! PFHpA2 363.0/319.0 Da Baseline a Missed l Wrong Peak PFHxS 2 399.0/99.0 Da Baseline Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After ferogeisridifStete N/A Baseline o Missed Wrong Peak Page 60 of 245 58 p. 48 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 02081 Ob\Samplesl_ong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-004 2/8/2010 9:16:45 PM PFCkinetexLong.dam 44 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0 .0 0 Results Summary A nalyte Peak Nam e IS Nam e PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS A rea 3433000 2433700 5444400 2369600 103610 5444400 128460 128460 128460 A n a ly te RT (Exp RT ) 1.36 (1.36) 2.33 (2.42) 2.32 (2.53) 3.31 (3.34) 4.09 (4.06) 4.10 (4.10) 4.75 (4.63) 5.28 (5.14) 5.73 (5.74) A nalyte Area 126150 13655 463860 12517 9351 16442 10245 7198 6854 C alc. C one (ng/mL) 0.791 0.073 0.814 0.079 0.074 0.046 0 .1 1 2 0.123 0.201 M odified? Mo No Mo No Mio No No No No -7 q ll z- f l Page 62 of 242 59  rv k e s1 Created with Anal^ftReporter Printed: 14/02/2010 6:41:00 PM IRC Summary A n alyte Peak Nam e PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 SJ3C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.35 (1.36) 1.31 (1.36) 2.26 (2.44) 2.28 (2.44) 2.32 (2.53) 2.32 (2.53) 0.00(3.34) 3.31 (3.34) 0.00 (4.06) 4.10 (4.06) 4.10(4.10) 4.75 (4.64) 4.75(4.64) 5.27(5.13) 5.72 (5.56) A rea 272960 3433000 4027 2433700 152800 5444400 0 2369600 0 103610 5664 2598 128460 2573 2375 M odified? No C alc (Exp) MRM R atio 2.16(2.38) R atio C o n firm s ID YES No 27.21 (50.17) No No 0.29(0.28) YES No 178.23(19.17) No No 0.33(0.32) YES No 11.74(22.53) No No 0.00(0.15) No No 189.31 (22.10) No No 0 .0 0 (0 .2 2 ) No No 11.08(1.86) No No 0.34 (0.32) YES No 0.25(0.29) YES No 12.54(3.08) No No 0.36(0.32) YES No 0.35(0.36) YES Page 63 of 242 60 JTl C o lu m b ia A nalytical Services" Quantitative Peak Review p. 50 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-004 Before After 61 Page 64 of 242 / ? C olum bia 3 A nalytical Services" Created with Anal^St^eporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation I:: r PFOA 2 413.0/369.0 Da u Baseline c Missed Wrong Peak I. PFNA2 463.0/219.0 Da c Baseline Missed Wrong Peak K1000911-004 l \ m \ .... -......... U1i / ............. j-JCv PFOS 2 499.0/99.0 Da o Baseline Missed g Wrong Peak PFDA2 513.0/269.0 Da u Baseline Missed Wrong Peak iVwv'y'As'/a!''W'"vr4 v )/ PFUnA2 563.0/269.0 Da u Baseline Missed u Wrong Peak PFDS2 599.0/99.0 Da c Baseline g Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline u Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak r*'-iGV&Wa \ PFTeA2 713.0/169.0 Da Baseline Missed c Wrong Peak Before After Page 65 of 242 62 C olum b ia A nalytical p. 52 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's ProjectsVPFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-004 2/9/2010 7:15:17AM PFCkinetexShort.dam 44 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 4821600 5317500 3224300 5317500 5317500 3224300 4331900 4331900 Analyte RT (Exp RT ) 1.27 (1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.23 (6.23) 6.33 (6.32) 6.33 (6.31) 7.67 (7.65) Analyte Area 120210 251660 187200 269020 67991 651470 3224300 4331900 Calc. Cone (ng/mL) 0.18 0.47 0.18 0.42 0.27 0.60 0.84 1.00 Modified? Mo No No No No No No No ((O Page 61 of 245 63 p. 53 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary A nalyte Peak Nam e PFBA2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.27(1.27) 3.60 (3.60) 4.26(4.26) 5.31 (5.31) 6.24 (6.22) 6.33 (6.32) 1.26 (1.26) 5.31 (5.30) A rea 120210 251660 68977 24871 215780 258640 4821600 5317500 M odified? No C alc (E xp) MRM R atio 1 .0 0 (1 .0 0 ) R atio C o n firm s ID YES No 1 .0 0 (1 .0 0 ) YES No 0.37 (0.40) YES No 0.09(0.10) YES No 3.17(3.03) YES No 0.40(0.40) YES No 40.11 (13.55) No No 19.77(13.06) No Page 62 of 245 64 Quantitative Peak Review p. 54 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000911-004 Before After Page 63 of 245 65 / ? C o lu m b ia O Analytical Services" p. 55 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation ""i : :: T ......... 'v ^ a I l ____ "L 3.60 I _L PFBA 2 213.0/169.0 Da l Baseline c Missed Wrong Peak 6 .2 4 PFPeA 2 263.0/219.0 Da Baseline u Missed Wrong Peak j K1000911-004 6 1 L__J PFBS2 299.0/99.0 Da G Baseline l Missed Wrong Peak / J*i H. ......... 5. PFHxA 2 313.0/119.0 Da Baseline j Missed Wrong Peak ;| i .............. ; "'"'j 6.- 3 This hage is not avalable PFHpA2 363.0/319.0 Da l Baseline Missed u Wrong Peak PFHxS2 399.0/99.0 Da Baseline : Missed u Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline j Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A c Baseline Missed Wrong Peak Page 64 of 245 66 ;&r$' p. 56 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810b\Samplesl_ong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-005 2/8/2010 9:24:18 PM PFCkinetexLong.dam 45 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3217400 2369400 5543300 2521100 124270 5543300 128020 128020 128020 Analyte RT (Exp RT ) 1.35 (1.36) 2.27 (2.42) 2.32 (2.53) 0.00(3.34) 4.10 (4.06) 4.09 (4.10) 0.00 (4.63) 0.00 (5.14) 0.00 (5.74) Analyte Area 126090 12750 490160 0 6795 3406 0 0 0 Calc. Cone (ng/mL) 0.841 0.071 0.844 N/A 0.036 0.026 N/A N/A N/A Modified? No No No No No No No No No -P S U 1 {C 7J[ Page 66 of 242 67 Created with Analyst ^ p o r te r Printed: 14/02/2010 6:41:00 PM IRC Summary A nalyte Peak Nam e PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 SJ3C-PFOS PFDA 2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDOA PFTrA 2 PFTeA2 RT (Exp RT ) 1.33 (1.36) 1.36(1.36) 2.29 (2.44) 2.28 (2.44) 2.32 (2.S3) 2.31 (2.53) 0.00 (3.34) 3.32 (3.34) 0.00 (4.06) 4.10 (4.06) 4.09 (4.10) 4.74(4.64) 4 .7 4 (4.64) 5.28(5.13) 5.72 (5.56) A rea 254060 3217400 3716 2369400 159700 5543300 0 2521100 0 124270 742 1217 128020 516 541 M odified? No C alc (E xp) MRM R atio 2.01 (2.38) R atio C o n firm s ID YES No 25.52(50.17) No No 0.29 (0.28) YES No 185.84(19.17) No No 0.33(0.32) YES No 11.31 (22.53) No No 0.00 (0.15) No No 0 . 0 0 ( 2 2 .1 0 ) No No 0 . 0 0 ( 0 . 2 2 ) No No 18.29 (1.86) No No 0.22(0.32) No No 0.00 (0.29) No No 0.00(3.08) No No 0.00(0.32) No No 0.00(0.36) No Page 67 of 242 68 Analytical Quantitative Peak Review 1.35 PFOA 1 413.0/169.0 Da Baseline u Missed n Wronn Peak 2.32 PFOS 1 499.0/80.0 Da u Baseline o Missed r Wronn Peak 4,10 13C-PFOA 417.0/372.0 Da Baseline Missed n Wronn Peak l 13C-PFOS 503.0/80.0 Da Baseline c Missed r , Wronn Peak 4.10 PFUnA 1 563.0/519.0 Da c Baseline u Missed i Wrono Peak 13C-PFUnA 565.0/270.0 Da a Baseline Missed n Wronn Peak___ p. 58 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-005 2.27 PFNA 1 463.0/419.0 Da c Baseline Missed n Wronn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed n Wrnnn Peak i PFDA 1 513.0/469.0 Da Baseline Missed r Wronn Peak 4.09 PFDS 1 599.0/80.0 Da Baseline Missed ___ n-WmnafiaK___ 13C-PFDA 515.0/470.0 Da n Baseline Missed n W rn n n Poak i2.31 13C-PFOS 503.0/80.0 Da Baseline Missed p Wrong Peak___ PFDoA 1 613.0/569.0 Da Baseline o Missed i . Wronn Peak 13C-PFDoA 615.0/169.0 Da Baseline Missed Wrnnn Poak PFTrA 1 663.0/619.0 Da Baseline Missed ___n Wrnnn Peak 130-PFDoA 615.0/169.0 Da Baseline Missed ___i : W ronn Peak___ PFTeA 1 713.0/669.0 Da u Baseline Missed __ 11 Wronn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed ___ n Wronn Peak Before After 69 Page 68 of 242 Created with Anal^fttim porter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation / 1.33 j Il . . i Z\ PFOA2 413.0/369.0 Da o Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak -nm,j : ,,,->i K1000911-005 1(Mij ,iuij1 i / W-34 - .. _ PFOS2 499.0/99.0 Da Baseline g Missed Wrong Peak PFDA 2 513.0/269.0 Da Baseline Missed Wrong Peak fifjfc, VOil PFUnA2 563.0/269.0 Da c Baseline Missed a Wrong Peak PFDS 2 599.0/99.0 Da Baseline Missed c Wrong Peak ^ - T ` T _ PFDoA 2 613.0/169.0 Da Baseline Missed Wrong Peak PFTrA2 663.0/169.0 Da Baseline Missed Wrong Peak - i ^ PFTeA2 713.0/169.0 Da Baseline a Missed g Wrong Peak Before After Page 69 of 242 70 / Columbia A rdivik al Services' p. 60 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-005 2/9/2010 7:24:50 AM PFCkinetexShort.dam 45 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 5145200 6060500 3305000 6060500 6060500 3305000 4658500 4658500 Analyte RT (Exp RT ) 1.28 (1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.24 (6.23) 6.33 (6.32) 6.33 (6.31) 7.67 (7.65) Analyte Area 127640 271900 196680 285590 71283 705430 3305000 4658500 Calc. Cone (ng/mL) 0.18 0.45 0.19 0.39 0.24 0.63 0.81 1.00 Modified? Mo No Mo Mo No No No No o I s l `b z f, ff< c Page 65 of 245 71 / C o lu m b ia A nalytical Son/ices" Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary A nalyte Peak Nam e PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS 2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.28(1.27) 3.60(3.60) 4.26 (4.26) 5.31 (5.31) 6.24 (6.22) 6.33 (6.32) 1.27 (1.26) 5.31 (5.30) A rea 127640 271900 75145 27335 249920 268850 5145200 6060500 M odified? No C alc (E xp ) MRM R atio 1.00(1.00) R atio C o n firm s ID YES No 1.00(1.00) YES No 0.38(0.40) YES No 0.10(0.10) YES No 3.51 (3.03) YES No 0.38(0.40) YES No 40.31 (13.55) No No 21.22(13.06) No Page 66 of 245 72 Sumbia m m s' Quantitative Peak Review p. 62 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000911-005 PFBA 1 213.0/169.0 Da l_- Baseline g Missed n Wronn Peak 4^ Ai 13C-PFBA 217.0/172.0 Da Baseline Missed n Wronn Peak 6.^3 ^ 1 .............I PFPeAi 263.0/219.0 Da u Baseline g Missed n Wronn Peak 5.31 :I 13C-PFHxA 315.0/270.0 Da c Baseline Missed n Wronn Peak 5.^1 PFBS 1 299.0/80.0 Da g Baseline a Missed i Wronn Peak - , 6.24 18 0 - P F H S 403.0/103.0 Da Baseline u Missed 11 Wronn Peak 5.^1 : 1 . ........ PFHxA 1 313.0/269.0 Da Baseline c Missed n Wronn Peak 6.^3 13C-PFHxA 315.0/270.0 Da u Baseline d Missed n Wronn Peak S.^3 PFHpA 1 363.0/169.0 Da l Baseline o Missed n Wrnno Peak 6.^3 13C-PFHxA 315.0/270.0 Da Baseline u Missed r Wronn Peak PFHxS 1 399.0/80.0 Da Baseline Missed n Wronn Peak '" i 7.67 :jj i 1 i S_180-PFHS 403.0/103.0 Da c Baseline Missed ___ __i ; Wronn Peak_____ 13C-PFDA 515.0/470.0 Da c Baseline Missed p Wronn Peak S 13C-PFDA 515.0/470.0 Da Baseline a Missed p Wronn Peak Before After 18 0 -P F H S 403.0/103.0 Da Baseline Missed n Wronn Peak :'l I 13C-PFDA 515.0/470.0 Da c Baseline a Missed _____ i-. Wronn Peak Page 67 of 245 73 p. 63 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 1.28 3.60 K1000911-005 'I N \ , r'J . 1 PFBA2 213.0/169.0 Da l Baseline Missed l Wrong Peak 6.24 PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak ,y, 6. PFBS2 299.0/99.0 Da Baseline Missed d Wrong Peak ) * Cfirf it ______ ] I I I PFHpA2 363.0/319.0 Da l Baseline Missed l Wrong Peak PFHxS 2 399.0/99.0 Da G Baseline Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da Baseline rj Missed Wrong Peak This image is not avaiable N/A c Baseline Missed Wrong Peak Page 68 of 245 74 Columbia Analytical Sen/kes p. 64 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-006 2/8/2010 9:31:50 PM PFCkinetexLong.dam 46 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFD0A PFTeA 1 13C-PFDOA IS Area 3009400 2154900 4825800 2224100 90602 4825800 110730 110730 110730 Analyte RT (Exp RT ) 1.36 (1.36) 2.32 (2.42) 2.33 (2.53) 0.00 (3.34) 4.12 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00 (5.74) Analyte Area 285230 Calc. Cone (ng/mL) 1.990 89642 0.387 922980 1.810 0 N/A h* /J 0 N/A 0 N/A 0 N/A 0 N/A Modified? No No No No No No No No No 4 A' *7 2 J f Ur I 10 Page 70 of 242 75 p. 65 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name RT (Exp RT) PFOA 2 1.37 (1.36) S_13C_PFOA 1.35(1.36) PFNA 2 2.31 (2.44) S_13C_PFNA 2.31 (2.44) PFOS 2 2.33 (2.53) S_13C-PFOS 2.34 (2.53) PFDA 2 0.00(3.34) S_13C-PFDA 3.31 (3.34) PFUnA2 0.00(4.06) -------------------------------------- j S_13C-PFUnA 4.10(4.06) PFDS 2 4.09 (4.10) PFDoA2 4.77 (4.64) S_13C-PFDoA 4.73 (4.64) PFTrA 2 5.28 (5.13) PFTeA2 5.54(5.56) Area 594510 3009400 28398 2154900 319260 4825800 0 2224100 0 90602 289 868 110730 122 147 Modified? Calc (Exp) MRM Ratio No 2.08(2.38) Ratio Confirms ID YES No 10.55(50.17) No No 0.32(0.28) YES No 24.04(19.17) No No 0.35(0.32) YES No 5.23 (22.53) No No 0.00(0.15) No No 0 .0 0 (2 2 .1 0 ) No No 0 .0 0 (0 .2 2 ) No No 27.26(1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00(3.08) No No 0.00 (0.32) No No 0.00 (0.36) No Page 71 of 242 76 Columbia A nalytical Quantitative Peak Review PFOA 1 413.0/169.0 Da Baseline Missed n W rnnn Peak 13C-PFOA 417.0/372.0 Da Baseline a Missed n Wrnnn Peak___ p. 66 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-006 2.32 1 PFNA 1 463.0/419.0 Da c Baseline Missed n W rnnn Peak 13C-PFNA 468.0/423.0 Da u Baseline Missed ___n Wronn Peak___ I 3.31 PFOS 1 499.0/80.0 Da Baseline Missed n W rnnn Peak 4.12 -S1B4bJPIP' 'PI 1 PFUnA 1 563.0/519.0 Da c Baseline l Missed ___ i W ro n n P e a k 13C-PFOS 503.0/80.0 Da Baseline Missed n W rnnn Peak 4.10 13C-PFUnA 565.0/270.0 Da Baseline l Missed n Wronn Peak "?! Vp'-i ` PFDoA 1 613.0/569.0 Da c Baseline Missed n W ronn Peak JL 13C-PFDOA 615.0/169.0 Da d Baseline Missed n W ronn Peak 4.7 PFDA 1 513.0/469.0 Da Baseline Missed n W rnnn Peak 13C-PFDA 515.0/470.0 Da a Baseline Missed t ; W ronn Peak PFDS 1 599.0/80.0 Da l Baseline Missed P Wronn Peak___ 13C-PFOS 503.0/80.0 Da Baseline Missed ___P Wrnnn Peak .piiLi */ij ./ i PFTrA 1 663.0/619.0 Da u Baseline Missed ___i Wrnnn Peak 130-PFDoA 615.0/169.0 Da u Baseline Missed ___ p W rn n n P p a k PFTeA 1 713.0/669.0 Da c Baseline u Missed ___ i W ro n n P eak 13C-PFDOA 615.0/169.0 Da u Baseline Missed ___ r W rn n n P eak Before After 77 Page 72 of 242 lytscai Ion Ratio Confirmation Created with Analjfet^eporter Printed: 14/02/2010 6:41:00 PM K1000911-006 Before After Page 73 of 242 78 jrt C o lu m b ia lL 3 kmfc. Services p. 68 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrum ent Name Ewan's ProjectsVPFCs 02081 OVSamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sam ple Name Acquisition Date Acquisition Method Injection Vial K1000911-006 2/9/2010 7:34:22 AM PFCkinetexShort.dam 46 Injection Volum e Sample Type Dilution Factor W eight to Volum e 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 13C-PFHxA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 4337300 5302300 3133500 5302300 5302300 3133500 4217300 4217300 Analyte RT (Exp RT ) 1.26 (1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.23 (6.23) 6.32 (6.32) 6.32 (6.31) 7.66 (7.65) Analyte Area 294470 813450 541850 861950 194210 1987900 3133500 4217300 Calc. Cone (ng/mL) 0.58 1.58 0.51 1.46 0.89 1.91 0.84 1.00 Modified? Mo No Mio No No No No No 4 fio 2. l h Page 69 of 245 79 p. 69 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM ;ic&! IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT) 1.26(1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.23 (6.22) 6.32 (6.32) 1.26 (1.26) 5.31 (5.30) Area 294470 813450 201250 76031 598650 774290 4337300 5302300 Modified? Calc (Exp) MRM Ratio No 1 .0 0 (1 .0 0 ) Ratio Confirms ID YES No 1 .0 0 (1 .0 0 ) YES No 0.37(0.40) YES No 0.09(0.10) YES No 3.08(3.03) YES No 0.39(0.40) YES No 14.73 (13.55) YES No 6.15(13.06) No Page 70 of 245 80 Gaiiirribf; Analytic Quantitative Peak Review I: : ; 1 PFBA 1 213.0/169.0 Da u Baseline c Missed n Wrnnn Peak 4.^ 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak p. 70 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000911-006 ...j 3.60 ""1 j . ...ll... PFPeA 1 263.0/219.0 Da d Baseline u Missed n Wrnnn Peak " ^ 5' ^ 13C-PFHxA 315.0/270.0 Da g Baseline c Missed n Wrnnn Peak J '[ CD .... Illin^ CO PFBS 1 299.0/80.0 Da l Baseline c Missed r Wronn Peak 6 .2 3 180-PFPIS 403.0/103.0 Da u Baseline Missed 11 Wrnnn Peak H '[ PFHxA 1 313.0/269.0 Da d Baseline Missed _____ n Wrnnn Peak_____ 13C-PFHxA 315.0/270.0 Da Baseline a Missed n Wrnnn Peak J 6.^2 PFHpA 1 363.0/169.0 Da g Baseline g Missed i Wronn Peak 6 13C-PFHxA 315.0/270.0 Da Baseline g Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da g Baseline u Missed r Wrnnn Peak 7 .6 6 180-PFHS 403.0/103.0 Da c Baseline c Missed n Wrnnn Peak ,--S 7 .^ 6 S 180-PFHS 13C-PFDA S 13C-PFDA 13C-PFDA 403.0/103.0 Da 515.0/470.0 Da 515.0/470.0 Da 515.0/470.0 Da u Baseline Missed c Baseline Missed u Baseline Missed g Baseline Missed _____ r Wrnnn Peak_____ _____ r- Wrnnn Peak_____ _____ n Wrnnn Peak_____ ________ n Wrnnn Peak__ Before After Page 71 of 245 81 mrmms Ion Ratio Confirmation 1.26 3.60 Created with A nalst Reporter Printed: 15/02/2010 2:34:00 PM K1000911-006 PFBA2 213.0/169.0 Da l Baseline a Missed l Wrong Peak 6.23 PFPeA2 263.0/219.0 Da c Baseline Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak !; I ! j-- ....... L ____ i T ,* i 'a __ PFHpA 2 363.0/319.0 Da Baseline o Missed Wrong Peak PFHxS2 399.0/99.0 Da d Baseline c Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Thismageismlstalle N/A a Baseline o Missed D Wrong Peak Page 72 of 245 82 JTC o lu m b ia m ly k a l S w w m s* P- 72 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrum ent Name Ewan's ProjectsVPFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sam ple Name Acquisition Date Acquisition Method Injection Vial K1000911-007 2/8/2010 9:39:24 PM PFCkinetexLong.dam 47 Injection Volume Sam ple Type Dilution Factor W eight to Volum e 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFD0A IS Area 3202200 2455900 5197700 2347900 91262 5197700 115230 115230 115230 Analyte RT (Exp RT ) 1.34 (1.36) 2.27 (2.42) 2.31 (2.53) 0.00(3.34) 4.10 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00 (5.14) 0.00 (5.74) Analyte Area 291950 93626 1045200 0 5919 0 0 0 0 Calc. Cone (n g /m L ) 1.910 0.357 1.900 N/A 0.047 N/A N/A N/A N/A Modified? No No No No Mo Mo No No No -V '1" <: ' ' n/ ^ 1j l f-rt Page 74 of 242 83 IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 SJ3C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 ices' p. 73 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM RT (Exp RT) 1.32(1.36) 1.34(1.36) 2.27(2.44) 2.27 (2.44) 2.30 (2.53) 2.30 (2.53) 0.00 (3.34) 3.28 (3.34) 0.00 (4.06) 4.09(4.06) 4.10(4.10) 4.74 (4.64) 4.74 (4.64) 5.03(5.13) 5.70 (5.56) Area 615900 3202200 33149 2455900 358110 5197700 0 2347900 0 91262 628 771 115230 401 154 Modified? Calc (Exp) MRM Ratio No 2.11 (2.38) Ratio Confirms ID YES No 10.97 (50.17) No No 0.35(0.28) No No 26.23 (19.17) No No 0.34(0.32) YES No 4.97(22.53) No No 0.00 (0.15) No No 0 .0 0 (2 2 .1 0 ) No No 0 .0 0 (0 .2 2 ) No No 15.42(1.86) No No 0.00 (0.32) No No 0.00(0.29) No No 0.00(3.08) No No 0.00(0.32) No No 0.00(0.36) No Page 75 of 242 84 C olom bia Analytical Servlets Quantitative Peak Review p. 74 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-007 Before After 85 Page 76 of 242 ervkoes' Ion Ratio Confirmation 1.32 Created with A nalst ^ p o rte r Printed: 14/02/2010 6:41:00 PM K1000911-007 PFOA 2 413.0/369.0 Da c Baseline a Missed u Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak Ij L : f " ____ PFUnA2 563.0/269.0 Da Baseline Missed c Wrong Peak PFDS 2 599.0/99.0 Da Baseline Missed a Wrong Peak PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak fM im PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak ,t . PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 77 of 242 86 /i C o lu m b ia AreM icai Servies p. 76 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrum ent Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sam ple Name Acquisition Date Acquisition Method Injection Vial K1000911-007 2/9/2010 7:43:55 AM PFCkinetexShort.dam 47 Injection Volum e Sample Type Dilution Factor W eight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 13C-PFHXA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 5005700 5999800 3203500 5999800 5999800 3203500 4572000 4572000 Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.32 (6.32) 6.31 (6.31) 7.65 (7.65) Analyte Area 344000 939100 551440 966840 222200 2035300 3203500 4572000 Calc. Cone (n g /m L ) 0.59 1.62 0.51 1.45 0.90 1.91 0.80 1.00 Modified? No Mo Mo No No Mo No No f^ "Z-/ 1i Page 73 of 245 87 p. 77 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.31 (5.31) 6 .2 2 (6 .2 2 ) 6.32 (6.32) 1.26 (1.26) 5.30(5.30) Area 344000 939100 214030 86700 650710 789230 5005700 5999800 Modified? Calc (Exp) MRM Ratio No 1 .0 0 (1 .0 0 ) Ratio Confirms ID YES No 1 .0 0 (1 .0 0 ) YES No 0.39 (0.40) YES No 0.09(0.10) YES No 2.93(3.03) YES No 0.39(0.40) YES No 14.55(13.55) YES No 6.21 (13.06) No Page 74 of 245 88 ICCiS"' Quantitative Peak Review 1,1r6 ;i \ p. 78 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000911-007 5.^) |3 } PFBA 1 213.0/169.0 Da i_ Baseline c Missed i Wronn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n Wronn Peak 6.31 PFPeA 1 263.0/219.0 Da Baseline Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak 5.^0 ir. t ; PFBS 1 299.0/80.0 Da g Baseline c Missed _____ n Wrnnn Peak_____ ll 180-PFFIS 403.0/103.0 Da Baseline Missed n Wrnnn Peak 5.^) PFHxA 1 313.0/269.0 Da Baseline Missed n Wrnnn Peak ...1 6.^2 13C-PFHxA 315.0/270.0 Da g Baseline c Missed _____ n Wrnnn Peak__ <6 | <6 ... PFHpA 1 363.0/169.0 Da l Baseline u Missed _____ i Wronn Peak_____ ;*j 6 .:!1 13C-PFHxA 315.0/270.0 Da u Baseline Missed n Wrnnn Peak 7.^5 PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Peak ! 7.65 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak | 7.65 ! Ii i1 S 180-PFHS 13C-PFDA S 13C-PFDA 13C-PFDA 403.0/103.0 Da 515.0/470.0 Da 515.0/470.0 Da 515.0/470.0 Da Baseline o Missed cj Baseline Missed Baseline Missed Baseline Missed _____ n Wronn Peak_____ _____ n Wrnnn Peak_____ _____ n Wrnnn Peak_____ _____ n Wrnnn Peak__ Before After Page 75 of 245 89 / T Columbia L ) Analytical Services" p. 79 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 128 . j : I i "! A. 3.59 1........ Jl . _ 4^ K1000911-007 i 5 | '-'i . . 5. .. .r-T ...... 'I k - : PFBA 2 213.0/169.0 Da l Baseline Missed l Wrong Peak , 6.2i2---- ----- 7 . . L . A ... PFPeA 2 263.0/219.0 Da Baseline c Missed c Wrong Peak f* r r, 2 .......... /J PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak I 1u*i. i ' - 5 - .... ;...... T ::........ I\ PFHpA 2 363.0/319.0 Da l! Baseline Missed Wrong Peak PFHxS 2 399.0/99.0 Da Baseline j Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. PFHxA 2 313.0/119.0 Da u Baseline Missed d Wrong Peak Ihisinageisnoli/aiate N/A Baseline Missed d Wrong Peak Before After Page 76 of 245 90 S i m m s '- p. 80 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrum ent Name Ewan's ProjectsVPFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sam ple Name Acquisition Date Acquisition Method Injection Vial K1000911-008 2/8/2010 9:46:57 PM PFCkinetexLong.dam 48 Injection Volume Sam ple Type Dilution Factor W eight to Volum e 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3095300 2232000 5319900 2106600 79208 5319900 93655 93655 93655 Analyte RT (Exp RT ) 1.38 (1.36) 0.00(2.42) 2.33 (2.53) 0.00 (3.34) 4.11 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00 (5.14) 0.00 (5.74) Analyte Area 34829 Calc. Cone (n g /m L ) 0.266 0 N/A 175120 0.323 0 N/A 3J39<- >, i t v h!0 0 N/A 0 N/A 0 N/A 0 N/A Modified? No No No No No No No No No 4 /V. 2^ (O Page 78 of 242 91 p. 81 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary A n alyte Peak Nam e PFOA 2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp R T ) 1.35(1.36) 1.34(1.36) 2.29 (2.44) 2.28(2.44) 2.30 (2.53) 2.33 (2.53) 0.00 (3.34) 3.31 (3.34) 0.00 (4.06) 4.11 (4.06) 4.13 (4.10) 4.74(4.64) 4.75 (4.64) 5.28(5.13) 5.56(5.56) A rea 69011 3095300 674 2232000 58307 5319900 0 2106600 0 79208 555 769 93655 361 500 M odified? No C alc (Exp) M RM R atio 1.98 (2.38) R atio C o n firm s ID YES NO 88.87(50.17) No No 0 .0 0 (0.28) No No 0.00(19.17) No No 0.33(0.32) YES No 30.38 (22.53) No No 0.00 (0.15) No No 0 .0 0 (2 2 .1 0 ) No No 0 .0 0 (0 .2 2 ) No No 22.57(1.86) No No 0.00(0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00(0.32) No No 0.00(0.36) No Page 79 of 242 92 Quantitative Peak Review p. 82 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-008 Before After 93 Page 80 of 242 i&rvices' p. 83 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation | 1.35 i mj PFOA2 413.0/369.0 Da l_ Baseline c Missed u Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak K1000911-008 :: A - / W3Q PFOS2 499.0/99.0 Da G Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed c Wrong Peak PFUnA2 563.0/269.0 Da u Baseline c Missed c Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da u Baseline c Missed u Wrong Peak Before After Page 81 of 242 94 :bia kal Srnrnrnr p. 84 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrum ent Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sam ple Name Acquisition Date Acquisition Method Injection Vial K1000911-008 2/9/2010 7:53:27 AM PFCkinetexShort.dam 48 Injection Volume Sam ple Type Dilution Factor W eight to Volume 5 Unknown 1.00 0 .0 0 Results Summary A nalyte Peak Nam e IS N am e PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S J8 0 -P F H S 13C-PFDA SJ3C-PFDA 13C-PFDA IS A rea 4745400 5417800 3103500 5417800 5417800 3103500 4525200 4525200 A n a ly te RT (Exp RT ) 1.26 (1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.23 (6.23) 6.32 (6.32) 6.32 (6.31) 7.65 (7.65) A nalyte Area 65074 97761 50550 122310 25656 339300 3103500 4525200 C alc. C one (n g /m L ) 0.07 0.17 0.06 0.16 0.06 0.32 0.78 1.00 M odified? No No No Mo Mo Mo Mo No 16 \\b 77 J it Page 77 of 245 95 Created with Anall/st^eporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT(ExpRT) 1.26 (1.27) 3.60(3.60) 4.25 (4.26) 5.31 (5.31) 6.23 (6.22) 6.32 (6.32) 1.26 (1.26) 5.30(5.30) Area 65074 97761 19403 12797 101750 130510 4745400 5417800 Modified? Calc (Exp) MRM Ratio No 1 .0 0 (1 .0 0 ) Ratio Confirms ID YES No 1 .0 0 (1 .0 0 ) YES No 0.38(0.40) YES No 0 .1 0 (0 .1 0 ) No 3.97 (3.03) YES No / H No 0.38(0.40) YES No 72.92(13.55) No No 44.30(13.06) No Page 78 of 245 96 p. 86 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review 126 K1000911-008 5.30 3.60 PFBA 1 213.0/169.0 Da u Baseline u Missed n W rnnn P eak 13C-PFBA 217.0/172.0 Da o Baseline Missed r W rnnn Peak PFPeA 1 263.0/219.0 Da Baseline Missed n W rn n n Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed r Wrnnn Peak PFBS 1 299.0/80.0 Da Baseline Missed i~i W r n n n P e a k PFHpA 1 363.0/169.0 Da u Baseline o Missed n W rnnn P eak 180-PFHS 403.0/103.0 Da Baseline Missed r Wrnnn Peak PFHxA 1 313.0/269.0 Da Baseline c Missed n Wrnnn Peak 6.552 0.1 13C-PFHxA 315.0/270.0 Da a Baseline Missed 11 W rn n n P e a k 7.5 PFHxS 1 399.0/80.0 Da Baseline u Missed n Wrnnn Peak 7.5 13C-PFHxA 315.0/270.0 Da Baseline g Missed n Wrnnn Peak 6.3 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak 7.i S_180-PFHS 403.0/103.0 Da j Baseline Missed __ i : Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da g Baseline g Missed ___n Wrnnn Peak S_13C-PFDA 515.0/470.0 Da l Baseline a Missed ___11Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed ___r- Wrnnn Peak___ Page 79 o f 245 97 Createci with Anal^sfReporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation K1000911-008 3.60 PFBA2 213.0/169.0 Da li Baseline c Missed l Wrong Peak PFPeA2 263.0/219.0 Da c Baseline Missed Wrong Peak PFBS2 299.0/99.0 Da c Baseline Missed Wrong Peak 6f . ^ ;c<(, : :r : A ....- - A /TM '" ; << c 6. 2.......; L^ l >-' 15 ........... ' bi l is ?o PFHpA 2 363.0/319.0 Da Baseline Missed c Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline a Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da Baseline u Missed c Wrong Peak Thisirageisnotsrraiable N/A Baseline a Missed Wrong Peak Page 80 of 245 98 JTi C o lu m b ia i L j ArmhkM $rvisies:' p. 88 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrum ent Name Ewan's ProjectsVPFCs 02081 ObVSamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sam ple Name Acquisition Date Acquisition Method Injection Vial K1000911-009 2/8/2010 9:54:30 PM PFCkinetexLong.dam 49 Injection Volum e Sam ple Type Dilution Factor W eight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3591100 2577600 6111900 2328400 118800 6111900 148650 148650 148650 Analyte RT (Exp RT ) 1.35 (1.36) 2.36 (2.42) 2.32 (2.53) 3.29 (3.34) 4.11 (4.06) 4.11 (4.10) 4.76 (4.63) 5.27 (5.14) 0.00(5.74) Analyte Area 36512 Calc. Cone (n g /m L ) 0.243 4#5"^ i*H ^pr38 jjb 178340 0.288 11853 0.077 18847 0.146 18820 0.047 14918 0.150 5329 0.065 0 N/A Modified? No Mo No No No No No Mo No V \ 2- Page 82 of 242 99 S e rv ic e s' p. 89 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary ; Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT) 1.35(1.36) 1.35(1.36) 2.26 (2.44) 2.28 (2.44) 2.31 (2.53) 2.33 (2.53) 0.00 (3.34) 3.31 (3.34) 4.10 (4.06) 4.11 (4.06) 4.11 (4.10) 4.75 (4.64) 4.75 (4.64) 5.28(5.13) 5.74(5.56) Area 64580 3591100 1301 2577600 59266 6111900 0 2328400 4197 118800 5011 5162 148650 1490 1133 Modified? Calc (Exp) MRM Ratio No 1.77 (2.38) Ratio Confirms ID No No 98.35 (50.17) No No 0.32 (0.28) YES No 638.79(19.17) No No 0.33(0.32) YES No 34.27 (22.53) No No 0.00 (0.15) No No 196.44 (22.10) No No 0.22(0.22) YES No 6.30 (1.86) No No 0.27(0.32) YES No 0.35(0.29) YES No 9.96 (3.08) No No 0.28(0.32) YES No 0.00(0.36) No Page 83 of 242 100 la al Services' Quantitative Peak Review Created with Analj/sf*$eporter Printed: 14/02/2010 6:41:00 PM K1000911-009 Before After 101 Page 84 of 242 p. 91 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation 1.35 " ...... PFOA2 413.0/369.0 Da u Baseline Missed u Wrong Peak ; :o 25 j'i PFNA2 463.0/219.0 Da c Baseline Missed Wrong Peak K1000911-009 i iiimni w. 1 PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA 2 513.0/269.0 Da Baseline Missed Wrong Peak PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da c Baseline a Missed Wrong Peak Before After Page 85 of 242 102 p. 92 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 OVSamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-009 2/9/2010 8:03:00 AM PFCkinetexShort.dam 49 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S J 8 0 -P F H S 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 5070300 5894400 3356000 5894400 5894400 3356000 4211300 4211300 Analyte RT (Exp RT ) 1.27 (1.27) 3.59 (3.60) 4.24 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65) Analyte Area 64061 97138 53329 120520 29132 351920 3356000 4211300 Calc. Cone (ng/mL) 0.06 0.15 0.06 0.14 0.07 MtV 0.31 0.90 1.00 Modified? No Mo No No No No No No (0 (I Page 81 o f 245 103 C olum b ia Analytical p. 93 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary A nalyte Peak Nam e PFBA 2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.27(1.27) 3.59(3.60) 4.26 (4.26) 5.30 (5.31) 6.22 (6.22) 6.31 (6.32) 1.27(1.26) 5.30(5.30) A rea 64061 97138 19574 11222 60914 136010 5070300 5894400 M odified? No C alc (E xp) M RM R atio 1.00(1.00) R atio C o n firm s ID YES No 1.00(1.00) YES No 0.37(0.40) YES No 0.09(0.10) YES No 2.09(3.03) No No 0.39 (0.40) YES No 79.15 (13.55) No No 48.91 (13.06) No Page 82 of 245 104 C olum b ia Analytical Services- Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Before After Page 83 of 245 105 p. 95 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation K1000911-009 PFBA2 213.0/169.0 Da l Baseline o Missed c Wrong Peak 3.59 PFPeA2 263.0/219.0 Da c Baseline Missed Wrong Peak j . 4i 6 ............ PFBS2 299.0/99.0 Da Baseline Missed u Wrong Peak Mjflli .5/3Q PFHxA 2 313.0/119.0 Da a Baseline Missed Wrong Peak This iriage is not svaiabie PFHpA2 363.0/319.0 Da u Baseline Missed l Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed a Wrong Peak SJ3C-PFDA 515.0/470.0 Da a Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline u Missed Wrong Peak Page 84 of 245 106 p. 96 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrum ent Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sam ple Name A cquisition Date Acquisition Method Injection Vial K1000911-010 2/8/2010 10:02:06 PM PFCkinetexLong.dam 50 Injection Volum e Sam ple Type Dilution Factor W eight to Volum e 5 Unknown 1.00 0.00 Results Summary A n alyte Peak Nam e IS Nam e PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFD0A PFTeA 1 13C-PFDOA IS A rea 3568900 2597200 5925100 2355200 96657 5925100 120520 120520 120520 A n alyte RT (Exp R T ) 1.37 (1.36) 2.29 (2.42) 2.35 (2.53) 0.00(3.34) 4.10 (4.06) 4.12 (4.10) 0.00(4.63) 0.00(5.14) 0.00(5.74) A n alyte Area 59571 40429 381100 0 6209 5707 0 0 0 C alc. C one (n g /m L ) 0.378 0.160 0.618 N/A 0.046 0.029 N/A N/A N/A M odified? No Mo No No No No No No No \N !U- 0 2- Page 86 of 242 107 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary A n aly te P eak N am e PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA 2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (E xp R T ) 1.37(1.36) 1.34(1.36) 2.28 (2.44) 2.28 (2.44) 2.36 (2.53) 2.33 (2.53) 0.00(3.34) 3.31 (3.34) 4.09 (4.06) 4.11 (4.06) 4.10(4.10) 4.73 (4.64) 4.75 (4.64) 4.98(5.13) 5.55 (5.56) A rea 117460 3568900 12233 2597200 124680 5925100 0 2355200 2022 96657 2297 1089 120520 360 233 M odified? NO C alc (Exp) M RM R atio 1.97(2.38) R atio C o n firm s ID YES No 59.91 (50.17) YES No 0.30(0.28) YES No 64.24(19.17) No No 0.33 (0.32) YES No 15.55 (22.53) No No 0.00 (0.15) No No 0 .0 0 (2 2 .1 0 ) No No 0.33 (0.22) No No 15.57(1.86) No No 0.40(0.32) YES No 0.00 (0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00 (0.36) No Page 87 of 242 108 Services' Quantitative Peak Review I 1.34 PFOA 1 413.0/169.0 Da u Baseline c Missed p W rnnn Peak 13C-PFOA 417.0/372.0 Da g Baseline Missed n Wrnnn Peak 2.33 Created with Ana lyif Reporter Printed: 14/02/2010 6:41:00 PM K1000911-010 2.29 L PFNA 1 463.0/419.0 Da Baseline Missed n W rnnn P eak 13C-PFNA 468.0/423.0 Da Baseline Missed I PFOS 1 499.0/80.0 Da Baseline Missed p W rnnn Peak PFUnA 1 563.0/519.0 Da u Baseline Missed i Wronn Peak ,:1' PFDoA 1 613.0/569.0 Da u Baseline Missed i ; W rnnn Peak 13C-PFO S 503.0/80.0 Da Baseline Missed n W rnnn Peak 4.11 PFDA 1 513.0/469.0 Da Baseline Missed n W rnnn P eak 13C-PFDA 515.0/470.0 Da Baseline g Missed n W rnnn Peak 13C-PFUnA 565.0/270.0 Da u Baseline u Missed n W rnnn Peak 4.75 l 13C-PFDOA 615.0/169.0 Da Baseline Missed m W rnnn Peak 4.12 .1 PFDS 1 599.0/80.0 Da l Baseline u Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed Wrnnn Peak___ ; in i2 PFTrA i 663.0/619.0 Da c Baseline Missed p W rn n n P e a k ______ 13C-PFDoA 615.0/169.0 Da c Baseline Missed n W rnnn P ea k iv . 'v I PFTeA 1 713.0/669.0 Da Baseline Missed p Wrong.Peak ..... 13C-PFDoA 615.0/169.0 Da c Baseline Missed _____n W rn n n P e a k ___ Before After Page 88 of 242 109 Ion Ratio Confirmation p. 99 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-010 Before After Page 89 of 242 110 C olum bia Analytical Servais p. 100 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-010 2/9/2010 8:12:36 AM PFCkinetexShort.dam 50 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHXA PFHpA 1 13C-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 5332700 6239000 3584700 6239000 6239000 3584700 4377000 4377000 Analyte RT (Exp RT ) 1.27 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 76518 130940 93172 156110 39615 680400 3584700 4377000 Calc. Cone (ng/mL) 0.08 0 .2 0 0.09 0.18 0.11 0.56 0.93 1.00 Modified? No No No No No No No No 4 ? l Page 85 of 245 111 Services' Created with Anal|/st*$eporter Printed: 15/02/2010 2:34:00 PM IRC Summary A n alyte Peak N am e PFBA 2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.27 (1.27) 3.59(3.60) 4.25 (4.26) 5.30(5.31) 6 .2 2 (6 .2 2 ) 6.31 (6.32) 1.26(1.26) 5.30(5.30) A rea 76518 130940 32685 14563 118750 273120 5332700 6239000 M odified? No C alc (Exp) MRM R atio 1 .0 0 (1 .0 0 ) R atio C o n firm s ID YES No 1 .0 0 (1 .0 0 ) YES No 0.35(0.40) YES No 0.09(0.10) YES No 3.00(3.03) YES No 0.40(0.40) YES No 69.69(13.55) No No 39.97(13.06) No Page 86 of 245 112 p. 102 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review 1 27 PFBA 1 213.0/169.0 Da c Baseline Missed r. W rn n n P e a k 4.?5 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak K1000911-010 5.30 3.59 jL PFPeA 1 263.0/219.0 Da Baseline c Missed n Wronn Peak 13C-PFHxA 315.0/270.0 Da a Baseline o Missed n W ronn Peak PFBS 1 299.0/80.0 Da c Baseline a Missed 11 W rnnn Pnak 180-PFHS 403.0/103.0 Da Baseline o Missed n W rnnn Peak .1 PFHxA 1 313.0/269.0 Da u Baseline Missed n W rnnn Ppak 130-PFHxA 315.0/270.0 Da c Baseline a Missed n W rnnn Ppak 6,31 PFHpA 1 363.0/169.0 Da i_ Baseline Missed n W rn n n Peak 13C-PFHxA 315.0/270.0 Da c Baseline Missed n W rnnn Pnak 7.6 PFHxS 1 399.0/80.0 Da U Baseline Missed 11 W rnnn Peak 7.6 180-PFHS 403.0/103.0 Da c Baseline u Missed! n W rnnn Peak 7.64 S_180-PFHS 403.0/103.0 Da l Baseline Missed r 1 W rnnn Poak 13C-PFDA 515.0/470.0 Da Baseline Missed ___n Wrnnn Peak___ SJ3C-PFDA 515.0/470.0 Da u Baseline Missed ___ n W rn n n P e a k___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak___ Page 87 of 245 113 Im V iu is Created with Anal$st1Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation : j ....... - f " : .....- K...I I C: . i1 1 K1000911-010 3.59. ----------------- 1 ______ . > ____ 4,-fc5 u. -! '".______ ___________ PFBA 2 213.0/169.0 Da l, Baseline Missed Wrong Peak PFPeA 2 263.0/219.0 Da Baseline Missed c Wrong Peak PFBS 2 299.0/99.0 Da Baseline Missed Wrong Peak 6.^2 t ... ::i ......- 1 1 _- ' ........ 1 m *.5........M.i.... SI | .. Ml f.S /ft PFHpA2 363.0/319.0 Da l. Baseline c Missed u Wrong Peak PFHxS 2 399.0/99.0 Da o Baseline Missed Wrong Peak S 13C-PFDA 515.0/470.0 Da Baseline Missed o Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Ite magesnotavalai N/A G Baseline Missed Wrong Peak Before After Page 88 of 245 114 p. 104 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrum ent Name Ewan's ProjectsVPFCs 02081 OtASamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sam ple Name A cquisition Date Acquisition Method Injection Vial K 1 0 0 0 9 1 1-011 2/8/2010 10:09:37 PM PFCkinetexLong.dam 51 Injection Volum e Sam ple Type Dilution Factor W eight to Volum e 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13 C -P F O S PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 3752400 2810900 6193900 2468000 130020 6193900 144860 144860 144860 Analyte RT (Exp RT ) 1.30 (1.36) 2.26 (2.42) 2.29 (2.53) 0.00 (3.34) 4.11 (4.06) 0.00 (4.10) 0.00 (4.63) 0 .0 0 (5 .1 4 ) 0.00 (5.74) Analyte Area 24615 8874 50866 0 4227 0 0 0 0 Calc. Cone (n g /m L ) 0.169 0.051 0.092 N/A 0.013 N/A N/A N/A N/A Modified? No No No No No No No No No %^ V,?ro\V\ J "lA . I U: to Page 90 of 242 115 iu m b ia Created with A n a ls t t ^ o r t e r Printed: 14/02/2010 6:41:00 PM IRC Summary A nalyte Peak Nam e PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S _13C -P F O S PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.31 (1.36) 1.37 (1.36) 2.33 (2.44) 2.24 (2.44) 2.31 (2.53) 2.30 (2.53) 0.00 (3.34) 3.28 (3.34) 0.00 (4.06) 4.10 (4.06) 3.91 (4.10) 4.70 (4.64) 4.74 (4.64) 5 .1 7 (5 .1 3 ) 5.56(5.56) A rea 41680 3752400 1504 2810900 14973 6193900 0 2468000 0 130020 182 252 144860 214 332 M odified? No C alc (E xp) M RM R atio 1.69 (2.38) R atio C o n firm s ID No No 152.44 (50.17) No No 0.17 (0.28) No No 3 1 6 .7 4 (1 9 .1 7 ) No No 0 .29(0.32 ) YES No 121.77 (22.53) No No 0 .0 0 (0 .1 5 ) No No 0.00 (22.10) No No 0 .0 0 (0 .2 2 ) No No 30.7 6 (1 .8 6 ) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0 .0 0 (0 .3 2 ) No No 0 .0 0 (0 .3 6 ) No Page 91 of 242 116 f % CofifSTSfeisI imJb Analytical Servians" Quantitative Peak Review p. 106 Created with Analyst Reporter Printed: 14/02/2 010 6:41:00 PM K1000911-011 2.26 PFOA 1 413.0/169.0 Da Baseline u Missed n Wronn Peak A PFOS 1 499.0/80.0 Da Baseline Missed i i W rn n n Peak 4.11 PFUnA 1 563.0/519.0 Da cj Baseline a Missed i/rnnn P e a k 13C -P F O A 417.0/372.0 Da u Baseline Missed n W ronn P aak I 2f 1 13C -P F O S 503.0/80.0 Da Baseline Missed n W rnnn Peak 13C-PFUnA 565.0/270.0 Da u Baseline d Missed ___ n Wrnnn Peak PFNA 1 463.0/419.0 Da Baseline u Missed n W rnnn P eak 13C-PFNA 468.0/423.0 Da Baseline Missed r Wronn Peak 3.28 PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed pi W r n n n P e a k k / 'r'i PFDS 1 599.0/80.0 Da Baseline o Missed ___ n Wrnnn Peak 13C-PFOS 503.0/80.0 Da d Baseline g Missed ___ n Wrnnn Peak PFDoA 1 613.0/569.0 Da Baseline c Missed r: W r n n n P s a k 13C-PFDoA 615.0/169.0 Da u Baseline Missed n W rnnn P eak I! K \if\ PFTrA 1 663.0/619.0 Da Baseline u Missed ___ r i W r n n n P a a k 13C-PFDOA 615.0/169.0 Da c Baseline Missed ____n W r n n n P e a k PFTeA 1 713.0/669.0 Da u Baseline Missed ___ n Wrnnn Peak 13C-PFDoA 615.0/169.0 Da c Baseline Missed n W rnnn P eak Before After Page 92 of 242 117 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation S-J ; ',_j : 1 .. ! ' ; p: 1,31 l 5,1 > PFOA 2 413.0/369.0 Da u Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak ".i . ........| Tr,* ml, PFUnA2 563.0/269.0 Da l Baseline n Missed d Wrong Peak 3^1 '1' 4$ 4 i SJl PFDS 2 599.0/99.0 Da D Baseline Missed Wrong Peak K1000911-011 (1 h f \ r 3l n / 1Vv.-,...n..........,... P F O S '2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak CflO ` ,ofi id 41 `,0 IS PFDoA2 613.0/169.0 Da u Baseline Missed u Wrong Peak am v .-,"<P ... PFTrA 2 663.0/169.0 Da Baseline Missed c Wrong Peak PFTeA2 713.0/169.0 Da Baseline c Missed c Wrong Peak Before After Page 93 of 242 118 C olum bia ices1 p. 108 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrum ent Name Ewan's Projects\PFCs 0 2 0 8 1 0 \S am ples S hort.w iff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-011 2/9/2010 8:22:11 AM PFCkinetexShort.dam 51 Injection Volum e Sample Type Dilution Factor W eight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHXA PFBS 1 1 8 0 -P F H S PFHxA 1 13C-PFHxA PFHpA 1 13C-PFHxA PFHxS 1 1 8 0 -P F H S S J80-P FH S 13C-PFDA SJ3C -PFD A 13C-PFDA IS Area 5632100 6722400 3712300 6722400 6722400 3712300 4862200 4862200 Analyte RT (Exp R T ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.31 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65) Analyte Area 94959 162240 61863 160800 31050 171000 3712300 4862200 Calc. Cone (n g /m L ) 0.10 0.23 0.06 0.17 0.06 <4.^ 0.13 0.87 1.00 Modified? Mo No Mo No No No No No n Page 89 of 245 119 Created with A n a ls t l ^ o r t e r Printed: 15/02/2010 2:34:00 PM IRC Summary A n alyte Peak Nam e PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA 2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.26(1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.22) 6.31 (6.32) 1.27(1.26) 5.30(5.30) A rea 94959 162240 23900 14347 355570 66893 5632100 6722400 M odified? No C alc (Exp) MRM R atio 1.00 (1.00) R atio C o n firm s ID YES No 1.0 0 (1 .0 0 ) YES No 0 .39(0.40 ) YES No 0 .0 9 (0 .1 0 ) YES No 11.45(3.0 3) No No 0 .3 9 (0 .4 0 ) YES No 59.31 (13.55) No No 41.81 (13.06) No Page 90 of 245 120 es* Quantitative Peak Review 1.26 PFBA 1 213.0/169.0 Da o Baseline a Missed n Wronn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n Wronn Peak p. 110 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM K1000911-011 3.59 i PFPeA 1 263.0/219.0 Da Baseline Missed n Wronn Peak 5.31 13C-PFHxA 315.0/270.0 Da Baseline o Missed n Wronn Peak PFBS 1 299.0/80.0 Da Baseline Missed i ~Wrnnn Peak 1 8 0 -P F H S 403.0/103.0 Da Baseline Missed n W rn n n Peak PFHxA 1 313.0/269.0 Da c Baseline Missed r Wrnnn Peak 13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHpA 1 363.0/169.0 Da Baseline u Missed r W rnnn P eak 13C-PFHXA 315.0/270.0 Da Baseline l Missed n W rnnn P&ak 7,65 PFHxS 1 399.0/80.0 Da c. Baseline Missed r: Wrnnn Peak 180-P F H S 403.0/103.0 Da u Baseline c Missed n Wrnnn Peak 7.65 S _ 1 8 0 -P F H S 403.0/103.0 Da Baseline u Missed r: Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed ___ n Wrnnn Peak S_13C-PFDA 515.0/470.0 Da u Baseline Missed ___ n Wrnnn Peak___ Before After 13C-PFDA 515.0/470.0 Da j Baseline c Missed __ n Wrnnn Peak Page 91 of 245 121 Ion Ratio Confirmation Created with A n a ls t Importer Printed: 15/02/2010 2:34:00 PM K1000911-011 PFBA2 213.0/169.0 Da u Baseline a Missed i_ Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed n Wrong Peak PFBS2 299.0/99.0 Da c Baseline Missed Wrong Peak PFHxA2 313.0/119.0 Da Baseline Missed Wrong Peak PFHpA2 363.0/319.0 Da u Baseline Missed c Wrong Peak PFHxS2 399.0/99.0 Da d Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline o Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After Tits inaje is nd avaiaWe N/A Baseline Missed Wrong Peak Page 92 of 245 122 jr t Columbia Analytical Servicer p. 112 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrum ent Name Ewan's Projects\PFCs 02081 OtASamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sam ple Name Acquisition Date Acquisition Method Injection Vial K1000911-012 2/8/2010 10:17:11 PM PFCkinetexLong.dam 52 Injection Volum e Sample Type Dilution Factor W eight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFDOA PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 4142900 3194300 7437500 2278600 131860 7437500 173360 173360 173360 Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.33 (2.53) 0.00 (3.34) 4.10 (4.06) 4.12 (4.10) 4.74 (4.63) 5.27 (5.14) 5.72 (5.74) Analyte Area 0 0 23249 0 11109 9537 9397 8079 8015 Calc. Cone (ng/mL) N/A Modified? No N/A Mo 0.044 No N/A No 0.067 No 0.031 No 0.065 No 0.096 No 0.175 No _______ . f Li- nr Page 94 of 242 123 C o llItT ilb iS Analytical S-ctrvk&s" p. 113 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.38(1.36) 1.37(1.36) 2.25 (2.44) 2.29 (2.44) 2.34 (2.53) 2.34 (2.53) 0.00(3.34) 3.31 (3.34) 0.00(4.06) 4.10(4.06) 4.10(4.10) 4.76 (4.64) 4.73 (4.64) 5.27 (5.13) 5.73 (5.56) Area 5690 4142900 702 3194300 6321 7437500 0 2278600 0 131860 2846 2076 173360 2658 3313 Modified? Calc (Exp) MRM Ratio No 0.00 (2.38) Ratio Confirms ID No No 0.00(50.17) No No 0 .0 0 (0.28) No No 0.00(19.17) No No 0.27(0.32) YES No 319.91 (22.53) No No 0.00 (0.15) No No 0 .0 0 (2 2 .1 0 ) No No 0 .0 0 (0 .2 2 ) No No 11.87(1.86) No No 0.30 (0.32) YES No 0.22 (0.29) YES No 18.45 (3.08) No No 0.33 (0.32) YES No 0.41 (0.36) YES Page 95 of 242 124 / ? C olum bia l L 3 Amilvtkal Stryke r Quantitative Peak Review Created with Analyif Reporter Printed: 14/02/2010 6:41:00 PM K1000911-012 Before After 125 Page 96 of 242 Ivtiol S^rvic^s1 Ion Ratio Confirmation p. 115 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM K1000911-012 1.38 PFOA 2 413.0/369.0 Da Baseline Missed l Wrong Peak PFUnA 2 563.0/269.0 Da u Baseline Missed c Wrong Peak ~-vV\A/'fvA/^v PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak Milj l ..-- 4 TM PFDS2 599.0/99.0 Da d Baseline Missed Wrong Peak PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak .'IjlXi| "no 4 ; r | ............ 5. 17........... -is sn si PFDoA2 613.0/169.0 Da C Baseline Missed Wrong Peak PFTrA2 663.0/169.0 Da a Baseline Missed u Wrong Peak PFTeA2 713.0/169.0 Da u Baseline c Missed u Wrong Peak Before After Page 97 of 242 126 p. 116 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-012 2/9/2010 8:31:46 AM PFCkinetexShort.dam 52 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 180-PFHS PFHxA 1 130-PFHxA PFHpA 1 130-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 5849700 6632000 3937200 6632000 6632000 3937200 4209400 4209400 Analyte RT (Exp RT ) 1.26 (1.27) 3.60 (3.60) 4.26 (4.26) 5.30 (5.31) 6.23 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65) Analyte Area 52298 52835 9103 41708 16414 56251 3937200 4209400 Calc. Cone (ng/mL) 0.03 0.06 0 .0 2 0.01 o.oo n&y 0.03 1.06 1.00 Modified? No No No No No No No No J i S' Page 93 of 245 127 ium bia sM q !Swmtm p. 117 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary 1 Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp RT) 1.26(1.27) 3.60 (3.60) 4.25 (4.26) 5.31 (5.31) 6 .2 1 (6 .2 2 ) 6.31 (6.32) 1.26(1.26) 5.30(5.30) Area 52298 52835 3676 5897 371420 18782 5849700 6632000 Modified? Calc (Exp) MRM Ratio No 1 .0 0 (1 .0 0 ) Ratio Confirms ID YES No 1 .0 0 (1 .0 0 ) YES No 0.40(0.40) YES No 0.14(0.10) YES No 22.63(3.03) no r t iL No 0.33(0.40) YES No 111.85(13.55) No No 159.01 (13.06) No Page 94 of 245 128 / Columbia i L j Analytical Services" Quantitative Peak Review p. 118 Created with Analyst Reporter Printed: 15 / 0 2 /2 0 1 0 2 :3 4 :0 0 PM K1000911 -012 Before After Page 95 of 245 129 p. 119 Created with Analyst Reporter >ia Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 1.26 "i/(/WJV Wv, PFBA2 213.0/169.0 Da Baseline Missed u Wrong Peak 3.60 PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak K1000911-012 W PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak This'mage is tol avaiabte PFHpA2 363.0/319.0 Da u Baseline c Missed l Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed a Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed c Wrong Peak Page 96 of 245 130 bm Ica! Services Created with Analy&l- F ^ o rte r Printed: 14/02/2010 6:41:00 PM Project Data File Result Table Instrum ent Name Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000 Sam ple Name Acquisition Date Acquisition Method Injection Vial K1000911-013 2/8/2010 10:24:46 PM PFCkinetexLong.dam 53 Injection Volum e Sam ple Type Dilution Factor W eight to Volume 5 Unknown 1.00 0 .0 0 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 130-PFDoA PFTeA 1 130-PFDoA IS Area 2570000 1802900 4902700 2235100 88183 4902700 107340 107340 107340 Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.33 (2.53) 0.00(3.34) 4.11 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74) Analyte Area 0 Calc. Cone (n g /m L ) N/A 0 N/A 27136 0.067 0 N/A 5155'"'' A ; \2(l0 jj 0 N/A 0 N/A 0 N/A 0 N/A Modified? No No No No No No No No Mo /A. 9 \i (0 Page 98 of 242 131 p. 121 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 1.41 (1.36) 1.37(1.36) 2.40 (2.44) 2.31 (2.44) 2.40 (2.53) 2.37 (2.53) 0.00 (3.34) 3.33 (3.34) 0.00 (4.06) 4.11 (4.06) 3.97 (4.10) 4.75 (4.64) 4.75 (4.64) 5.24 (5.13) 5.71 (5.56) Area 9706 2570000 438 1802900 7912 4902700 0 2235100 0 88183 120 605 107340 389 690 Modified? Calc (Exp) MRM Ratio No 0.00 (2.38) Ratio Confirms ID No No 0.00 (50.17) No No 0 .0 0 (0.28) No No 0.00 (19.17) No No 0.29(0.32) YES No 180.67 (22.53) No No 0.00(0.15) No No 0 .0 0 (2 2 .1 0 ) No No 0 .0 0 (0 .2 2 ) No No 17.11 (1.86) No No 0.00 (0.32) No No 0.00 (0.29) No No 0.00 (3.08) No No 0.00 (0.32) No No 0.00(0.36) No Page 99 of 242 132 Quantitative Peak Review 1.37 p. 122 Created with Analyst Reporter Printed: 14/02/2010 6 41:00 PM K1000911-013 2.31 PFOA 1 413.0/169.0 Da u Baseline u Missed i Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline a Missed ___11 Wrnnn Peak___ 2.37 PFOS 1 499.0/80.0 Da c Baseline Missed n Wrnnn Peak Vr1S 1 13C-PFOS 503.0/80.0 Da Baseline Missed ri W rn n n P p a k PFNA 1 463.0/419.0 Da Baseline c Missed n Wrnnn Peak__ _ j'v/- PFDA 1 513.0/469.0 Da Baseline Missed n W rnnn P sak 13C-PFNA 468.0/423.0 Da u Baseline Missed n Wrnnn Peak___ 13C-PFDA 515.0/470.0 Da Baseline Missed n W rnnn P oak 2.37 PFUnA 1 563.0/519.0 Da Baseline o Missed i Wrnnn Peak___ 13C-PFUnA 565.0/270.0 Da c Baseline Missed n Wrnnn Peak___ PFDS 1 599.0/80.0 Da Baseline Missed ___i i Wrnnn Peak___ 13C-PFOS 503.0/80.0 Da o Baseline Missed n Wrnnn Peak V :,y f'iv'y'.WY' ' ..'A' PFDoA 1 613.0/569.0 Da c Baseline Missed i 1Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline c Missed n Wrnnn Peak PFTrA 1 663.0/619.0 Da a Baseline Missed _____n Wrnnn Peak___ 130-PFDoA 615.0/169.0 Da Baseline c Missed ___n W rnnn Peak___ PFTeA 1 713.0/669.0 Da Baseline Missed , Wrnnn Peak_____ 13C-PFDOA 615.0/169.0 Da Baseline Missed r Wrnnn Peak Before After 133 Page 100 of 242 jrf C o lu m b ia Analytical Services" p. 123 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation i 1.41 j .jjmffffflifi i ,,,,j PFOA2 413.0/369.0 Da u Baseline Missed Wrong Peak PFNA 2 463.0/219.0 Da c Baseline o Missed Wrong Peak K1000911-013 I uvia| PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak ind ril PFDA2 513.0/269.0 Da d Baseline l Missed d Wrong Peak PFUnA 2 563.0/269.0 Da cj Baseline Missed Wrong Peak JL97 PFDS2 599.0/99.0 Da d Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak /JL PFTeA 2 713.0/169.0 Da u Baseline Missed u Wrong Peak Before After Page 101 of 242 134 p. 124 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 02081 OXSamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial K1000911-013 2/9/2010 8:41:17 AM PFCkinetexShort.dam 53 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 13C-PFHxA PFBS 1 180-PFHS PFHxA 1 13C-PFHxA PFHpA 1 13C-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA S_13C-PFDA 13C-PFDA IS Area 2880400 3715800 2930800 3715800 3715800 2930800 3692800 3692800 Analyte RT (Exp RT) 1.27 (1.27) 3.60 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65) Analyte Area 32645 75618 8028 69215 16510 58653 2930800 3692800 Calc. Cone (ng/mL) 0.05 0.19 0.03 0.12 0.06 /il0.05 0.90 1.00 Modified? No No No No Mo No Mo No 'i v-'t 7 (Q ,(< Page 97 of 245 135 / C o lu m b ia A n a lytic a l Son/ices" p. 125 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary A n alyte Peak Nam e PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT (Exp R T ) 1.27(1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.21 (6.22) 6.31 (6.32) 1.26 (1.26) 5.30 (5.30) A rea 32645 75618 4049 6602 310350 21493 2880400 3715800 M odified? No C alc (Exp) MRM R atio 1.00(1.00) R atio C o n firm s ID YES No 1.00(1.00) YES No 0.50 (0.40) No No 0.10(0.10) YES No 18.80(3.03) No J -fU i No 0.37 (0.40) YES No 88.23(13.55) No No 53.68 (13.06) No Page 98 of 245 136