Document 0gvX10Ya7R75KRkOZMNr9edmO

FOR DU FONT USE ONLY AR226-2890 Copies toufl E. I. du Font de Neaours and Company Uaskell Laboratory for Toxicology and Industrial Medicine Elkton Road, He-ark. Delaware 19711 HASKELL LABORATORY REPORT NO. 866-80 Material Tested ~Haskell No. 13,384" Ocher Codes/Synonyms Study Initiated/Completed 7/i7/o - a/is/yo INHALATION MEDIAN LETHAL COMCENTRATIQN (LC50) he purpose of this investigation was to determine the 4-bour LC50 In male albino Crl:CD rats. Procedures; ofHHHIjin A" General: Groups of 10 male, albino Crl:CD rats. approximately 8 weeks old^and weighing '.'corn 224 to 293 g, were exposed via inhalation to atmospheres air for single .4br periods. Except during exposure, rats werenoueaoTn suspended stainless-steel 're mesh cages. Purina Certified Rodent Chow i!f5002 and water were suppli'eA ^d libitum. Duving exposure all raca were observed, clinical signs note4 '-"^ chamber temperature monitored. Following exposu.: , rats were weighed and observed daily (excluding weekends) for a 14-day recovery period. Generation: The sample was liquified by heating to approximately 100''C. A 50 cc plastic syringe wrapped in heating tape was loaded with liquified sampi'1 acd placed on syringe drive. The sample was syringe driven through a Spraying Systems Nebulizer,, The aerosolized stream sprayed into a heated, 3 neck, CcsigaaTy-SimffTzed. Does not contain TSCA CBI round boi-con flask. Dilution air was added and the resultant vapor/aerosol m lecture went through heated glass connectors into the top of a 20 1 glass [ chamber containing the test r. ! Analytical: Chamber samples were collected approximately every 1/2 hr by drawing a known volume of chamber atmosphere through 2 tandem midget iaplngers. The inpingerB contained acetone (ACS grade) as the trapping solvent. Samples were analyzed using a Hewlett Packard 5730A g&a chromato- graph equipped with a flame ionizatlon detector. The column was a 6' x 1/4" O.D. glass coil packed with 20X Neopentyl and Glycol Sebacate on 60/80 mesh chroaosorb FAH. Oven temperature w^s programmed tc hold at 80C for 2 minutes then increase 8C a minute to 220C and bold at that temperature for 4 minutes ^^^H^^Uconcentrations were based on the second lareesl^ofiak^^^ome^^ out incheG.C. (-hroaatograph. This peak is identified ^(HH^^^^^B.^ on the basis of the G. C. peak height area and the reporter sample^^^^^^^ conposicion. Chamber atmoBohereconaentrations were *'ien calculated and expressed as the equivalentjI^IBHBjconcencration (mg/1). Results: Observations; During all exposures rats exhibited reduced response to sound, irregular respiration, salivation, wet fur, and tearing from eyes. In exposures ^ ia.2 og/1 rats exhibited occasional gasping and varying degrees <.f dark red ocular and nasal discharge. In correlation to dosage, rats exhibited slight to severe weight loss, lasting 24-96 hrs post-exposure followed by normal weight gain* All deat-hs occurred 24-96 hrs post-exposure. Mortality Data; fencracion ng/I (range) 3.5 (2.1-4.4) 13.3 (9.2-15.9) 18.2 (12.1-23.2) 35.7 (27.3-51.4) Standard Deviation g/l + 0.8 7 2.0 7 4.0 7 7.5 Fractional Mortality ()? deaths/^ exposed) 0/10 1/10 2/10 5/10 The LCSO** wsa calc ilated to be 35.3 mg/1 with a lower limit of 23.8 mg/1 and an upper limit of 790.4 ag/1 with 95Z confidence limits. Cp3nyS,,.I^.D^no<o.----TSCACBI .Summary: Groups of 8 week old Crl:CD male rats were exposed to vapors of a|||l----[)for single 4 hr periods. The LC50** was calculated to be 35.3 mg/1 ^^H^^ffldence limits and a range of 23.8 - 790.4 mg/1). Clinical signs observed during exposure included reduced response Co sound, irregular respiration, salivation, wet fur, and tearing r'-"n eyes. A dark red discharge was exhibited from eyes and nose of rats exposed to ^ IS.2 Bg/1. Slight to severe weight loss was observed 24-96 hrs post-exposure in accordance with dosage and followed by normal weight gain. All deaths occurred 24-y6 hrs post-exposure. * CcapoaitiOD; ** D. J. Finney, Probit Analysis, 3rd Ed., 1971, Cambridge University Press. + this exposure was the highest concentration obtainable using the previously mentioned generation procedures and conditions. Report by; \^A ^ wk.^l \JJoseph C. Haaili Technologist jca;jrg Study Director; Approved by: JUL L-. ^fe^^JLg Gerald L. Kennedy Chief, Acute Investigations Section Bo A. Burgess amber 1.0. 1980 3 - Con,pnn,Sa^ed.t>.e>n-c^"TSCACB.