Document 0eVx1eDm1DdKgwb3L6D49vqx
CHRONIC TOXICITYTO FRESHWATER INVERTABRATES (Daphniamagna)
TEST SUBSTANCE
IdentityP:effluorooctanaocicda,mmonium saltm;ay alsobe referrteod as PFOA ammonium saltA,mmonium perfluorooctanoateF,C116,FC-126, FC-1 69,or FC-143. (Octanoicacid, pentadecafluoro-a,mmonium saltC,AS # 3825-26-1)
Remarks: The 3M productionlotnumber is264. The testsample is'FC143. It'psuritywas notsufficientclhyaracterizedt,hough current informationindicatesitisa mixtureof96.5 - 100% testsubstance and 0 3.5% C6, C7, and Cg perfluoroanalogue compounds. The 3M production lotnumber is264.
METHOD:
Method: USEPA-1982, OECD 1981. Type: Semi-StaticLife-CyclTeoxicity GLP: No Year completed: 1984 Species: Daphnia magna Supplier:In-housecultures3,M EnvironmentalLab, St Paul,MN Analyticalmonitoring:Temperature,pH and dissolvedoxygen Exposure period:21 days Statisticamlethods: Probitanalysis(foracutetoxicitvyalues)and Moving Average Angle Method forchronicstudy. Statisticwsere recalculatebdy ASci Corp.,Duluth,MN in1998. NOECs and LOECs were generatedusingToxstat.Reproductionwas normal and homogenous thereforeDunnett'sTest was used. Fisher'sTest was used forsurvival.IC5os(reproductionw)ere generatedusing lCp program. SurvivalEC5os were generated usingTrimmed spearman-Karber. Test organism age: < 24-houroldneonates fortestinitiation. Pretreatment: None
Test conditions:
Dilutionwater:Aerated carbon-filterweedllwater
Dilutionwater chemistry (sampled one month priorto study
initiation)
hardness: 240 mg/L as CaC03
alkalinity: 230 mg/L as CaC03
pH:
7.8
Conductivity: 340 @imhos/cm
COD:
< 0.4mg/L
Stock and testsolutionpreparation:Informationot available
Renewal rateE:verytwodays Exposurevessels2:50-mLglassbeakerscontaining approximately200 mL testsolution.The depth was approximately 5 cm. Beakers were covered withwatch glasses Number of replicates4: Number of testorganisms per replicate5: Loading: 1 daphnid per 40 mL Number of concentrations:6 plusa blankcontrol
Feeding:A suspension offishfood (troucthow) and yeast containing5 mg dry solidsper one mL ofmixturewas fed on a daily basis Lighting: Cool-whitefluorescentlights.Intensitryanged 50-100 ftc. Photopedod of 16-hourslight8,-hoursdark witha 20-minute transitiopneriod.
Water chemistry of new and old solutionsduring the study: PH and dissolved oxygen: Informationotavailable Test temperature range (0- 21 days):22+ 20C
Element basis:Survivaland reproduction.
RESULTS
Nominal Element
concentrations:Blank control,5,8,13, 22, 36,and 60 value: 14-day NOEC (survival=)60 mg/L
14-day NOEC (reproduction=)8 mg/L 21 -day NOEC (survival=)22 mg/L 21-day NOEC (reproduction=)22 mg/L 14-day ICFO(reproduction=)40 (28-48)mg/L 21 -day IC5o(reproduction=)43 (35- 46) mg/L
mg/L
Allelement values based on mean measured concentrations
Biologicalobservations Survival:Allsurvivingfirsgtenerationdaphnids appeared normal attesttermination.Survivalinthe 36 and 60 mg/L treatmentswas statisticaslilgynificantdliyfferenftrom the negativecontrolgroup. Reproduction: Daphnids inthe controlstartedproducingneonates on Day 7. Reproductionwas statisticaslilgynificantdliyfferenftrom the control(Dunneft'sa)tthe 13,22, 36, and 60 mg/L concentrationsafter14-days,and inthe36 and 60 mg/L test solutionsat 21-days.
Summary ofPercentMortality
Nominal
Conc.,mg/L Day 14
Day 21
Negative
0
0
Control
5.0
0
5
8.0
0
20
13
0
20
22
0
20
36
0 1 25 1
60
5
I- --80
Reproduction after 21 -days
Nominal Number of Mean Live
Concentration, Surviving Young /Adult
mg/L
Daphnids Daphnid(+SD)
NegativeControl 20
165+13
5.0
19
145+29
8.0
16
138+52
13
16
131+38
22
16
130+11
36
15
108+24
1
60
1 4 1 36+12
CONCLUSIONS
There were no adverse effectson survivalor reproductionDaphnia magna exposed to the testsubstance at concentration<22 mg/L for21 days.
Author and/orsubmitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133
DATA QUALITY
Reliability:Klimischranking3. Study was apparentlywell conducted according to the methodology availableatthe time oftestinitiation.
Informationwas notavailableon testsubstance purity.The raw data forthis study could not be located,and some key study elements could notbe evaluated at thistime.
REFERENCES
This study was conducted atthe 3M Company EnvironmentalLaboratory,St Paul,MN. Recalculationofthe effectand no-effectconcentrationwsas performed atASci Corporation,Duluth,MN
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
OTHER
Last changed: 5/25/06-
LR
ENVIRONMENTAL
LABORATORY
DATA SUMMARY REPORT ON THE'TESTS FOR
ACUTE & CHRONIC TOXICITY OF FLUOROCHEMICALS
TO DAPHNIA MAGNA (WATER FLEA)
(9972512600)
TEST SUBSTANCE
Description: FC--145 1,.O'r2-ip4
Additional Identificationt STATIC ACUTE TEST
Begin: 817-ISq- End: 8/4/04
Dose Range: Z-S, 40, JpS) Ioo Temperature:
Duration: )41814ouris
100) Z-50.04w 16 so w3tt
Results:
-
24-Hr. EC 50
(95% Confidence
Limits) :.4(1c(31do4'lZ-)
1
-
48-Hr.
EC 50
(95%
C.L.):
2-tolo (7-al- WI)t"
RENEWAL CHRONIC TEST
Begin: 8/'1/84 End:
Duration:
Dose Range: 5) 0)132).Z@3)(,P)(POK50
Temperature: ZJ,:t IOC
?-I -IAY@5
Resultss
Young Reproduction
Impairment
- Approximate NOEC
- MATC (Limits)CM-MJ;TC-
-
.14 -Day
EC 50
(95% C.L.)
- Z-1-Day EC 50 (95% C.L.)
Chronic/Acute Ratios
I &s III(IS-W W,5 (Z&-45)
.38
4Z.)
Application Factors
(t4ATC/Acute EC 50)
Enve Safety Factor
(MATC/EEC)
Adults Mortality
"III m8Lk >W
40 (51-SZ)
A &@a4;s+;casl@tikgicavwzic-s+waa- recormed, twv@k
-2-
ACCEPTABILITY OF CHRONIC TEST
The D. maana control' populat ion have met the following quality criterias
- Adults Mortality:
A'O*"C (max. 20%)
- First Brood Releases
@/jAm (max. 9 days)
- Cumulative No. Young per Adult (14-Day):YZain. 20)
- Cumulative No. Young-per Adult (21-Day):146min. 40-60)
- Ephippia:
"A*_(none)
- D.O. Saturations
BZZ(aLn. 601)
- pH Level:'
S.oto2(max. +0.5
from initial)
COMMENTS
Reported and Approved 7bby@: ,@ Dat*t
L4 &I V
C@A L@
I
A% L
TABLE I. SUMMARY OF ACUTE TOXICITY OF
145
T 0 DAPHNIA MAGNA UNDER STATIC EXPOSURE CONDITIONS
CHEMICAL TREATMENT
24-HR. EXPOSURE
4
MORTALITY
(mg/1)
CONTROL
0
0
0
0
IC)O
0
livo
Z,50
)4oo
40
qS
EC 50 (95% CONFIDENCE mg/l
(2 LIMITS)
(1) (2)
Data are averages of'two replications for,each treatment with ten (10) d replicate. A total of twenty daphnids wdre used per treatment.
EC values were calculated using logistic regression methods (Probit An 50
ENVIRONMENTAL
LABORATORY
TABLE'IL,
SUMMARY OF CHRONIC TOXICITY OF
TO DAPHNIA MAGNA UNDER RENEWED STATIC
rr--i461 LiTr2jo4
EXPOSURE CONDITIONS
CHEMICAL TREATMENT
14-DAY EXPOSURE
YOUNG REPRODUCTION
ADULT
IMPAIRMENT (1)
MORTALITY (1)
21-DN. YOUNG REPRO
IMPAIRMENT
CONTROL
IPO
EC so (95% CONFIDENCE
mg/]L
(2 LIMITS)
0
. 3(P foe
0
0
0
0
0 0
> 100
40
k4kes+ 4et.,+CD"CCD@
(1) Data &re averages of four replications for each treatment with five (5) daphn total of twenty daphnide were used per treatme n t.
(2) (3)
EC values were calculated using logistic regression methods (Probit Analyzi 50
ceaelmttledu@55itMtovim Aqevor- Amle- Mekuza
EC'SD V&lue-S U)ere
116
075.9
. -z@ 15--1-
0) fdl-il
Gc,7'9-1-41
90bi'O
l@i
gobl'o
go I
L.b9
ez
7-A
;Iairi%gsaiyslaslr
vefy" oulmn-Yot@
CA,k A117;=26P
IEst bKm
CMT
(o 4-
0. Oo@,54.;, 0,oo!sz, 0 L)od22
1Z5C):tI
o,ooqz-@
C((O E34-
19 1:L01001501-
C)IOO(5
(@e
C)10 1
0 i-S
C)i
115 oooot-,,.*
150
2.q -:5(0I-tZ 0.0c)oc@,
SUMMARY
MTE/RRR
ENVIRONMENTAL
LABORATORY
1-3-84
OF EXPERIMENTAL
DESIGN AND CONDITIONS FOR CONDUCTING
RENEWAL CHRONIC EFFECTS TESTS WITH DAPHNIDS
Project: Fate of Fluorochemicals - Phase Il (9972512600)
Exposure:
Type - renewal static Renewal frequency - every Duration - 14-21 days
2 days
Test Species:
Daphnia magna or -Daphnia pulex'(use of D.-*magna is pieferred)
Age of test-animals
12+12 hrs instars (neonates)
No. animals/beaker
5
No. of replicate test beakers per concentration
4
Total No. animals per concentration - 20
Test Vessel: 250 ml glass beaker (Pyrex) covered with a watch glass Test Solution Volume: 200 ml
Loading Ratio: One animal per 40 ml
Dilution Water Supply:
Aer.ated carbon-filtered well water (Bldg. 2) of
consistent chemical quality. Water hardness
classification: hard (hardness
250 mg/L as CACO 3
Untreated Controls: 100% well water
Toxicant boi6 Ranae: Minimum of five logarithmic concentrations (by weight)
Test Conditions:
Temperature
- 22+2 0 C (68-750F)
Light Quality - Type - ambient laboratory light
(cool-white fluorescent)
- Intensity - ambient lab levels (50-100 fc)
- Photoperiod - 16 hrs light; 20 minutes
transition period
Aeration During Test - None
I Foo d and Feedina Regime:
A suspension of fish food (trout chow) and yeast (Fleischmann's) containing 5 mg dry solids per one ml of mixture, on a daily basis.
Measurements and Observations: Effects (noted daily and recorded on renewal days) - No. young, live and dead; brood size, and no. broods - No. dead adults - Immobility, presence of unhatched eggs or eph.ippia, and other signs of stress water Quality (of dilution water and of replicates of old test solutions made on renewal days) - Physical: temperature, D.O., and pH - Chenticali analytical measurements. of toxicant concentrations in initial stock and in old test solutions (optional)
Validity of Test: The control population of D. maona- should meet the following requirements: - Mortality of adults - maximum 20% - First brood release - maximum 9 days - No. young per adult (14 days) - minimum" 20 - No. young per adult after 21 days - minimum 60 - Ephippia - none - D.O. saturation - minimum 60% throughout the test - pH - maximum +0.5 units from initial
Treatments of Results:
48-Hr Acute Test
- 24-hr ECso - 48-hr EC 50 with 14-21 Day Chronic
95% confidence TestC7@@
limits
- Approximate no observed effects concentration ONOEC"
- 14- and/or 21-day EC 50 with 95% confidence limits. - maximum acceptable toxicant concentration *KATCN
- Chronic/acute ratios
- Dose/response curve based on cumulative mortality of adults
- Safety factor
References:
- USEPA-1982. Env. Effects Test Guidelines. EPA560/6-82-002 - OECD-1981. Guidelines for Testing of Chemicals - ASTM-(latest drafts, 1983). Proposed Standard Practice for
Conducting Renewal Chronic Effects Tests with Daphnids
*Final results are calculated from mean values of cumulative no. young per adult, or cumulative mortality of adults.
Definitions:
Daphnia Chronic Toxicity Test - A 14-21 day experimental study of the survival, growth and reproduction of asexually-reproducing Daphnia beginning with-12+12 hr old organism.
Significant - *Statistically significantly different* refers to pZO.05 with respect to the control.
Approximate significant
NOEC - The highest test concentration that will produce no observed effect on reproduction of young or adult survival.
Chronic EC (s) - The values of toxicant concentrations which impair so
reproduction of young or induce adult mortality by 50%.
MATC '- The highest concentration of toxicant that has no adverse ef fect on survival, growth, ahd reproduction of a species.
GM-MATC is the calculated geometric mean between the highest test concentration having no significant effect and the lowest test concentration having a significant effect.
Application Factor (AF). The quotient of the chronic MATC divided by the acute 48-hr EC so (MATC/Acute EC 50 ). "AF" is a number used to estimate concentration of toxicant that will not cause significant effect to a population during chronic exposure.
Environmental Safety Factor - The ratio of the chronic HATC with the expected environmental concentration (MATC/EEC$. Safety factors >20 at the predicted exposure level are desirable to have sufficient confidence in the environmental safety assessment.
B-23-84
ENVIRONMENTAL LABORATORY IPINAL REPORT
LAB REQUEST NO* 92055
REQUESTER NAME: RRR. DEPt:-0222 PROJECT NO: 06 DATE RECEIVED: 8-16-84 DESC'.0 JUNE CARBON-FILTERED
WELL WATER
CONTRACT LAB:
CONTRACT LAB COST:
0
3,M E-L HOURS:
3
EST CHPLT DATE: 8-24-84
DATE COMPLETED: 8-24-84
SA"LE I
DATE 7@06m84
CODE RM 44
DESCRIPTION
2
DI
WATER.
TEST
PH RESMCL COND TOT-HARD TOT-ALI TS SH3-1 SOL-F COD-LOV TOT-COLIF SPC
PH RES-CL COND SPC
RESULT -----------
7,8 <0*02 MCIL 340 UNHOS/CM 240 MG/L 230 NG/L 270 NO/L <0*4 MCIL 0.2 MG/L <Oe4 KG/L, NEGATIVE 54 PER ML
6.6 <0.02 MGIL\----1.8 UKH()S/Cg 14 PER ML
=.CONTRACT LAB APPROVED AND SUBMITTED BY --Llll000 DATEI)
August10,1998
AScl Corporation
4444dnrparBkoulevarDdu,luthM,innesot5a5811
(218)722-4040
Fax(218)722-2592
Ms. Susan Beach 3M Environmental Technology and SafetyServices 935 Bush Avenue Building2-3E-09 PO Bo;L 33331- St.Paul,MN 55133-3331
Dear Sue:
FC-143 Daphnia magna reproductiondata for concentrationsabove the survivalNOEC were not includedduring hypothesistestingforthereproductionNOEC and LOEC. EPA guidance stipulatetshatconcentrationwshich significantleyffectsurvivalshouldnot be includedin hypothesistestingforreproduction.The exclusionof such concentrationussually resultsin a more powerful and appropriatestatisticaanlalysis.
Sinrc@eeryel,y,
Joe Dierkes BiologistA,SCI
Title: File:
FC-143 DAPHNIA MAGNA 21 DAY REPRODUCTION
fc2ld
Transform:
NO TRANSFORMATION
ANOVA Table ----------------------------------------------------------------------------
SOURCE
DF
ss
ms
F
----------------------------------------------------------------------------
Between
4
115876.3000
28969.0750
1.0871
Within (Error)
15
399732.5000
26648.8333
----------------------------------------------------------------------------
Total
19
515608.8000
----------------------------------------------------------------------------
(P-value = 0.3980)
Critical F
= 4.,8932 (alpha = 0.01, df = 4,15) = 3.0556 (alpha = 0.05, df = 4,15)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha 0.05)
Press any key to continue or ESC to return to menu...
Title: File:
FC-143 DAPHNIA MAGNA 21 DAY REPRODUCTION
fc2ld
Transform:
NO TRANSFORMATION
Dunnett's Test - TABLE 1 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
GROUP
IDENTIFICATION
----- --------------------
TRANSFORMED
MEAN -----------
MEAN CALCULATED IN
ORIGINAL UNITS
T STAT
------------------ ------
SIG 0.05 ---
1
CONTROL
825.7500
825.7500
2
5 MG/L
722.5000
722.5000
0.8945
3
8 MG/L
647.7500
647.7500
1.5420
.4
13 MG/L
630.5000
630.5000
5
22 MG/L
625.5000
625.5000
1.7348
----------------------------------------------------------------------------
Dunnett critical value 2.3600
(1 Tailed, alpha 0.05, df 4,15)
Press any key to continue...
Title: File:
FC-143
DAPHNIA MAGNA fc2ld
21 DAY REPRODUCTION Transform:
NO TRANSFORMATION
Dunnett's Test - TABLE 2 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF
GROUP
IDENTIFICATION
REPS
----- -------------------- -------
MIN SIG DIFF
t OF
(IN ORIG. UNITS) CONTROL ---------------- -------
DIFFERENCE FROM CONTROL ------------
1
CONTROL
4
2
5 MG/L'
4
272.4182
33.0
103.2500
3
8 MG/L
4
272.4182
33.0
178.0000
4
13 MG/L
4
272.4182
33.0
195.2500
5
22 MG/L
4
272.4182
33.0
200.2500
----------------------------------------------------------------------------
Press any key to continue or ESC to return to menu...
Title: File:
FC-143
DAPHNIA MAGNA fc2ld
21 DAY REPRODUCTION Transform:
NO TRANSFORMATION
Shapiro - Wilk's Test for Normality ----------------------------------------------------------------------------
D = 399732.5000 W = 0.9767
Critical W = 0.8680 (alpha = 0.01 , N = 20) W = 0.9050 (alpha = 0.05 , N = 20)
----------------------------------------------------------------------------
Data PASS normality test (alpha-= 0.01). Continue analysis.
o*7e/@ te ro
Press any key to continue
or ESC to return to menu...
Title: File:
FC-143
DAPHNIA MAGNA fc2ld
21 DAY REPRODUCTION Transform:
NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance ----------------------------------------------------------------------------
Calculated El statistic Data PASS Bl homogeneity
6.7904
(P-value = 0.1474)
test at 0.01 level. Continue analysis.
----------------------------------------------------------------------------
Critical B = 13.2767 = 9.4877
(alpha = 0.01, df = 4) (alpha = 0.05, df = 4)
Press any key to continue or ESC to return to menu...
Title: File:
FC-143
DAPHNIA MAGNA FC14D
14 DAY REPRODUCTION Transform:
NO TRANSFORMATION
Dunnett's Test
- TABLE 1 OF 2
---------------------------------------------------H-o-:-Co-n-t-r-o-l<-T-r-e-a-tm-e-n-t---------
GROUP
IDENTIFICATION
TRANSFORMED MEAN
MEAN CALCULATED IN ORIGINAL UNITS
T STAT
SIG 0.05
----- -------------------1
-----------
------------------ ------ ---
CONTROL
359.2500
359.2500
2 3
5 MG/L
311.2500
311.2500
1.0073
4
8 MG/L 13 MG/L
264.7500 228.5000
264.7500
1.9830
5
22 MG/L
224.2500
228.5000
2.7437
6
36 MG/L
191.2500
224.2500 191.2500
'2.8329
7
60 MG/L
115.2500
115.2500
3.5254 5.1203
----------------------------------------------------------------------------
Dunnett critical value 2.4600
(1 Tailed, alpha 0.05, df [used] = 6,20)
(Actual df = 6,21)
Press any key to continue...
Title: File:
FC-143
DAPHNIA MAGNA FC14D
14 DAY REPRODUCTION Transform:
NO TRANSFORMATION
Dunnett's Test
- TABLE 2 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
GROUP
IDENTIFICATION
N'UM OF REPS
MIN SIG DIFF (IN ORIG. UNITS)
k OF CONTROL
DIFFERENCE FROM CONTROL
----- -------------------- ------- ---------------- ------- ------------
1
CONTROL
4
2
5 MG/L
4
117.2286
32.6
48.0000
3
8 MG/L
4
117.2286
32.6
94.5000
4
13 MG/L
4
117.2286
32.6
130.7500
5 6
22 MG/L
4
117.2286
32.6
135.0000
36 MG/L
4
7
60 MG/L
4
----------------------
117.2286. 117.2286
32.6 32.6
168.0000 244.0000
-------------------------------------------------------
Press any key to continue or ESC to retu
Title: File:
FC-143
DAPHNIA MAGNA FC14D
14 DAY REPRODUCTION Transform:
NO TRANSFORMATION
ANOVA Table ----------------------------------------------------------------------------
SOURCE
DF
ss
ms
F
----------------------------------------------------------------------------
Between
6
152796.3571
25466.0595
5.6071
within (Error)
21
95377.5000
4541.7857
----------------------------------------------------------------------------
Total
27
248173.8571
----------------------------------------------------------------------------
(P-value
0.0013)
Critical F = 3.8117 = 2.5727
(alpha-= 0.01, df = 6,21) (alpha = 0.05, df = 6,21)
Since F > Critical F REJECT Ho: All 6qual (alpha 0.05)
Press any key to continue or ESC to return to menu...
Title: File:
FC-143
DAPHNIA MAGNA FC14D
14 DAY REPRODUCTION Transform:
NO TRANSFORMATION
Shapiro - Wilk's Test for Normality -------------------------------*---------------------------------------------
@D = 95377.5000 W = 0.9434
Critical W = 0.8960 (alpha = 0.01 , N = 28) W = 0.9240 (alpha = 0.05 , N = 28)
---------------------------------------------------------------------------Data PASS normality test (alpha 0.01). Continue analysis.
Press any key to continue or ESC to return to menu...
Title: File:
FC-143 DA PHNIA MAGNA FC14D
14 DAY REPRODUCTION Transform:
NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance ----------------------------------------------------------------------------
Calculated Bl statistic = 10.3740
(p-value = 0.1098)
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
----------------------------------------------------------------------------
Critical 13= 16.8119 = 12.5916
(alpha = 0.01, df = 6) (alpha = 0.05, df = 6)
Press any key to continue or ESC to return to menu...
Summary of Fisher's Exact Tests ----------------------------------------------------------------------
NUMBER
GROUP
IDENTIFICATION
EXPOSED
----- ------------------------- -----------
NUMBER
SIG
DEAD
0.05
----------- -----
1
CONTROL
20
0
5 MG/L
20
2
1
8 MG/L
20
4
3
13 MG/L
20
4
4
22 MG/L
20
4
5
36 MG/L
20
5
6
60 MG/L
20
16
--------------------------------------------------------------------------
Press any key to continue...
-------------------S-u-mm-a-r-y--o-f--F-i-sh-e-r-'-s--E-x-a-c-t --TI-e--s--t--s--------------------------
GROUP
IDENTIFICATION
----- ---------------------
NUMBER
EXPOSED ---------
NUMBER
DEAD -----------
SIG
0.05 -----
1
CONTROL
20
0
2
5 mg/l
20
0
8 mg/l
20
0
3
13 mg/l
20
0
4
22 mg/l
20
0
5
36 mg/l
20
0
6
60 mg/l
20
1
----------------------------------------------------------------------------
Press any key to continue ...
ENTER WOULD
THE NUMBER OF MORTALITIES 5 20 20 20 25 80
AT EACH CONCENTRATION:
YOU LIKE THE AUTOMATIC TRIM CALCULATION(Y/N)?
Y
DATE:
8/7/1984
CHEMICAL: FC-143
TEST NUMBER: I
DURATION: SPECIES:
21 DAYS D.MAGNA
RAW DATA: CONCENTRATION(MG/L) NUMBER EXPOSED: MORTALITIES:
SPEARMAN-KARBER.TRIM:
5.00 100 5
8.00 100 20
20.00*
13.00 100 20
-
22.00 100 20
36.00 100 25
60.00 100 80
SPEARMAN-KARBER
ESTIMATES:
EC50:
44.57
95k LOWER CONFI35ENCE:
40.88
95k UPPER CONFIDENCE:
48.60
--------------------------------------------
7 -----------------------------------
WOULD YOU LIKE TO-HAVE A COPY SENT TO THE PRINTER(Y/N)?
@ld
Toxicant/Effluent: FC-143
Test Start Date: 8/7/1984
Test Species: D.MAGNA
Test Duration:
14
DATA FILE:
Test DAY
Ending
Date:
8/21/198
-----------------------------------------------------------------------
Conc. ID
Number Replicates
Concentration
Response
Std.
Pooled
MG/L
Means
Dev.
Response Means
-----------------------------------------------------------------------
1
4
2
4
0.000
359.250
30.739
359.250
3
4
5.000 8.000
311.250
45.959
311.250
4
4
5
4
13.000 22.000
264.750 228.500
107.952 111.075
264.750 228.500
6
4
36.000
224.250
48.072
224.250
7
4
60.000
191.250 115.250
41.548
191.250
---------------------------------------------------2-6.-5-8-8-----1-1-5-.-25-0------
The Linear Interpolation Estimate:
39.6711
Entered P Value: 50
-----------------------------------------------------------------------
Number of Resamplings:
80
The Bootstrap Estimates Mean: Original Confidence Limits:
Expanded Confidence Limits:
Resampling time in Seconds:
Press
80-Resamples Generated
39.1710 Lower: Lower:
Standard
27.6757 20.4784
Deviation:
6.7021
Upper:
48.1897
Upper:
53.3009
1.32 Random Seed: -309057854
Any Key to Corit-inue
Toxicant/Effluent: FC-143
Test Start Date: 8/7/1984
Test Species: D.MAGNA
Test Duration:
21
DATA FILE:
Test DAY
Ending
Date:
8/28/84
/CR
Ics-a l@re(
-----------------------------------------------------------------------
Conc.
Number
Concentration
Response
Std.
ID
Replicates
MG/L
Means
Dev.
Pooled Response Means
-----------------------------------------------------------------------
1
4
2
4
0.000 5.000
825.750 722.500
65.383 143.412
825.750 722.500
3
4
4
4
8.000 13.000
647.750 630.500
242.861 212.111
647.750
5
4
6
4
22.000 36.000
625.500 523.000
66.556 129.956
630.500 625.500 523.000
7
4
60.000
155.250
35.753
155.250
-----------------------------------------------------------------------
The Linear Interpolation Estimate:
43.1869
Entered P Value: 50
-----------------------------------------------------------------------
Number of Resamplings:
80 80 Resamples Generated
The Bootstrap'Estimates Mean: Original Confidence Limits: Expanded Confidence Limits: Resampling time in Seconds:
Press
42.7226 Lower: Lower:
Standard 35.0020 30.0910
Deviation:
2.9224
Upper:
46.3442
Upper:
48.2386
1.26 Random Seed: -523848694
Any Key to Cont7inue