Document 0LzRkwyg2jKQxGRvXE04oN83b
HR226 - 3258
440
TRADE SECRET
Study Title H-25434: Static, Acute, 48-Hour Screening Test to
Daphnia magna Laboratory Project ID: DuPont-11409
AR226 -3258 DuPont11409
AuThoR: Laurie D. Bouchelle Alan Semel, M.S.
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STUDY COMPLETED ON: October 2, 2002
PERFORMING LABORATORY: EL du Pont de Nemours and Company Haskell Laboratory for Health and Environmental Sciences Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050
work Request Nuvo: (EN
stupy Cope Numser:(IY
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Page 1017
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Company Sanitzed. Does not contain TSCA CBI
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Duron 11409
CERTIFICATION
`We, the undersigned, declare that this report provides an accurate evaluationofdata obtained
from this study.
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Substance Tested: (EY Svnonvms/Codes: + H-25434 `Haskell Number: 25434
" `Known Impurities: Not characterized Physical Characteristics: Off-white, milky semi-opaque aqueous dispersion
Stability: The test substance appeared to be stable under the conditionsofthe study; no evidenceof instability was observed.
`Sponsor: E.L du Pont de Nemours and Company Wilmington, Delaware 19898 USA.
Study Initiated Completed: September 24, 2002/ (see report cover page) In-Life Initiated/Completed: September 24, 2002/ September 26, 2002
e Company Sanitized. Does not contain TSCA GBI
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SUMMARY
`The acute toxicityofH-25434 to the determined in an unaerated, 48-hour,
water static
lea, test.
Daphnia
magna
(less
than
24
hours
old)
was
m"Tehaensttuedmypewraastucroendoufc2t0e.d6wCit(hr4ancgoenocfe2n0t.r5a-ti2o0n.s7oCf).H-2O5n4e3t4esatncdhaamdibleurtiwoanswautseerdcpoenrtrtoelstat a
ccoonntcreonltraantdiotnhewi1t.0h, 1100taesntdo1rg0a0nmigs/msLitnesetaccohnccehnatmrbaetri.onsBawseerde oclneavriswuiatlhonbsoecrovlatoiroanst,estthestwaartt.er
`The 1000 mg/L test concentration was cloudy within acceptable limits during the exposure.
at
test
start.
All
water
quality
parameters
were
aEnxdpo1s0u0r0eomfgw/aLtreersufllteaesditno0t,he0,wa0t,e1r0,coanntdrol10a0nd%niommmiobniallitHy-,2r5e4s3pe4ctciovnecleyn,traatttihoensenodfo1f, 4108,h1o0u0r,s. N`noomiimnmaolbciolnicteyntorrastuibolnecthaaulsienfgfenctosiwmemroebisleietnyian ttheestweantderwcaosni1r0olmtge/sLt.orgTahneislmosw.estThneomhiingahlest ccoonncceennttrraattiioonnscawuesrienugs1ed00fo%ricmamlocublialtiitoyn aotftEesCtseonvdalwuaess. 1000 mg/L. Nominal H-25434
The results are summarized as follows:
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Nominal concentrations of H-25434, mg/L 48-hour ECso (95% fiducial limits) for
| dilution water conirol, | 234 (85 to 592)
1,
10, 100, and 1000
H-25434, based on nominal
concentrations, mg/L*
`H-25434
using the
ewxahtiebritfeleda,lDoawpchonnicaermnafgonr aaq(ulaetsiscthhaazna2r4d"hoinurasn
oulnda),erated,
48-hour,
static,
acute
test
* FPerltieer,sWh.aHw.ndaanMtdarWeienbreerO,rCgaLr,isEmdss.. (E1P9A85/)G.ODMAe-t$h5o.d01sf3o.r MUneitaedsStutahrseiEAncnvuitgreonTmeontxalioPrfcotEeficftetinotnysAg0ency, Environmental MonitoringandSupport Laboratory, Cincinnati, Ohio.
~~ * Smm(reWct.hGho.edkLsa,naJdni,dsC,elv1ea.5ml.eunaHttuisgo,nhoRe.fs,,dMaaotnrad.cMoE.cnAkv.,iRrLo.enwaminesdn,tREaadlbsTe)r,,poWp.2s2(-139i9a9.3nc)dA.RmoieArsssiklecAsasssnoienSsgosgcmeiceeonytltyo,gfiAocrSaTlTehsMatSzinaTgrPdaun1nd1d7eM9a,rteTrSiaClAs:, Philadelphia.
ree meee
reer
CompanySanitzed. Doesnotcontain TSCA Cai
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MATERIALS AND METHODS
A. Test Solution Preparation Tsoelsuttisounbssotfan1c,e 1s0o,lu1t0i0o,nsanwder1e0p0r0empgar/eLdwbeyredirpercetpaadrdeidtiboynoadfdHin-g25t4he34aptporodpirluitaitoenawmaoteurn.toTfetsetst material to HLWW in 1-L beakers and stirring for approximately 30 minutes. The water control a1n0d00thmeg1/.L0,te1s0tacnondce1n0t0rmatgi/oLn wteasst ccolnocuednytraatttieosntsstwaertr.e clear with no color at test start. The
B. Dilution Water
`Dainldsuetiaolnedwattoerbeodrriogcikn.ateTdhferhoamrtdhneesHsaosfketlhle LHabLorWaWtoriysadwjeullstwehditcoh aips p3r7o8x-ifmeeattedleyep10a0n-d14is0cmags/edL
tashrCoaugChOa;gbryetehnesfalnodwf-iplrtoerpotortrieomnoedveadidriotni,oannodffCialtCelre.d tThhreouHghL5W0-W, 1i0s-,thaenndaScr-aptemd,fipltaesrssetdo
rpoelmyovvienyplarcthilcourliatdees.pip`iTnhge.waTtheerdiisluhteiaotnedwaotrecrhiisllaendaalsyzaepdprtowpircieatyeeaarnldy dfiosrtmraibjuotredantihornosugahndaged
cations, metals, otal organochlorine and organophosphatepesticides, and polychlorinated
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`biphenyls. The dilution water meets. OECD and ASTM specifications.
C. Test Organism Culture Dbeaapkhenrisa(1m0agpenrabweearkeerraetarceudltautreHaisnkietilaltiLoanb)owrhaitcohrycoinnt2a5i0ne-dmL20(01 mpeLr obera1ke0r0)0 omrL1o0f0s0er-amtLePdy,rex `fainldtetrreodutHcLhWowW(hYeClTd)atm2i0x.t9urCe.(sDtaapnhdanriddiszewdertoe 1f7ed00ontoa2d1a0i0lymbga/siLstwoitatlhsaolyiedass)ta,ncdertehael lgeraeveens iaslgeaq,uSievleanlaestntotru0m.1c-a0p.r2imcogrntuottaulmo,ragtanaitcactearobfo6n2p,e5r00dacpehlnlisd/)m.L N(tehoenacotmebsiunsaetdioinnotfhisYCteTst awnedrealga eSiscskntehsasn,2i4njhuoryu,rsanodldabannodrmwaelrieticeosllweecrteednfortosmeetnhea1n2dtehpbhriopopdioafw2e8r-ednaoyt obledinpgarpernotdduacpehdnibdyst.he `4p8a-rhenoturdapprhentiedsst.perNiood.aduDlatpilmimnoibaimlaitgynwaawsesreeeindeinnttihfeiecdubltyurleasbeulsseodnftohretcesutlitnugredubreiankgertsheand test chambers.
D. Test Methods
Four nominal concentrations anda dilution water control were concentrations were 1, 10, 100, and 1000 mg/L H-25434.
used
in
this
study.
The
nominal
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DuPont-11405
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uPsyerdexas bteesatkecrhsam(b2e5r0s-.mLO)necornetpaliinciantge 2te0s0t cmhLaomfbteerstwassoulsuteidonpe(r6t8e-sctmcotnecsetnstorlauttiioonn dweiptthh) were
10 daphnids in cach chamber. The test chambers were covered witha glass plate during the test.
`duDraipnhgnitahemtaesgtn. aAndedointaitoenso,fledsaspthhnaind2s4tohotuesrtsosloldu,twieonrsewuassediniintitahtiesdsatfutdeyr.miDxaipnhgniofdtshweerteestnot fed scroiltuetriioonnsfwoarsthceomepffleectted(.imImmomboibliitlyi)tywaasnda lbaechkaovfiorreaalctoibosnertvoaatpipolniscawteiroenmoafadegednatillye.stTihmuelus,
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cDoinstsroollvaedndoxteysgtesnubcsotnacnecntercaotnicoenn,trpaHt,ioansn.d tTehmepsereamteuraesuwreermeemnetasswuerreedtiankaelnl rbeepfloirceatdeaspohfntihdes were a`wdedreednoatt taeesrtastteadrtd,uarnidngatthteestteestndanodr awtertoetadlisipmomsoebdiolfiityniannaarpepprloipcraitaetcehmamabnenre.r aTtestetstsoelnudt.ions
~
REFERENCES
1. OCrhgeamniicsaaltsi:on20fo3r,E1c7oJnuolmyi1c99C2o.-Operation and Development (OECD). Guideline for Testing
2. AComnedruicctainngSoAcciuettye fToorxTiecsittiynTgeasntsdwMiattherFiiaslhses(,AMSaTcMro)i.nve(r1i9c8b8)r.ateSst,anadnadrdAmGpuhiidbeiafnosr. E729-88a. Annual Book ofASTM Standards, Vol. 11.04.
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Company Saniized. Does not contain TSCA CBI
125434: Static, Acuts, 48-Hour
SoongTetioDophniamagra
wow
TABLE 1
DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES
`Nominal
Test Initiation (0 hours)
H-25434 Dissolved
Concentration | (mg
Oxygen 2
p
Test Completion (48 hours
Dissolved
Oxygen
(mg
p
`Water Control 8s
10
86
80 79
87 87
80 80
10 100
86 86
77 77
87 87
80 80
1000
86
77
88
79
a Measurement performed at 24 hours due to total immobility.
TABLE? IMMOBILITY OF Dapimia magna IN AN UNAERATED, STATIC, ACUTE, 48-HOUR TEST
WITH H-25434
Nominal H-25434 Concentration
(ng) `Water Control
10 10 100 1000
Numberof Dead Test Organisms / NumberofTest Organisms at Study Start
oo 10
10 10
010 10
010 110
10/10
10/10
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Company
Sanitized.
i------ Does not contain TSCA
Cai
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