Document 0LMwr9g55jRNgXM1ZMOvwRM

PROPOSAL TO MONSANTO COMPANY TITLE: The Degradation of Polychlorinated Biphenyls by Fungi and Anaerobic Bacteria PRINCIPAL INVESTIGATOR: Dr. Chun C. Lee (SSN 521-52-6704) INSTITUTION: New York University Medical Center Institute of Environmental Medicine Laboratory for Environmental Studies 550 First Avenue New York, New York 10016 DURATION OF PROPOSED WORK: June 1, 1979 through May 31, 1982 TOTAL AMOUNT REQUESTED: $154,931 <^?.c5^-g---______ Chun C. Lee Research Assistant Professor Institute of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 Merril Eisenbud Associate Director Institute of Environmental Medicine New York University Medical Center Tuxedo, New York 10987 Institute of Environmental Medicine New York University Medical Center 550 First Avenue New York, New York 10016 Thomas^A.f ^itzgerald. Director Office^of Grants Administration and Institutional Studies 550 First Avenue New York, New York 10016 HONS 013879 OBJECTIVES This research will seek to isolate and cultivate fungi and anaerobic bacteria from a variety of aquatic environ ments, which are able to grow in the presence of polychlorinated biphenyls and degrade the polychlorinated biphenyls. After such cultures have been established, the rate and mode of polychlorinated biphenyl degradation by these cultures will be examined, both in vitro and in situ. INTRODUCTION This study is proposed to investigate the role of fungi and anaerobic bacteria in polychlorinated biphenyl (PCB) degradation. Because of their low solubility in water, high specific gravity, and high adsorbability, PCBs in aquatic environments tend to accumulate in reducing or anaerobic sediments of lakes, rivers, estuaries and coastal zones (3,4,9). Evidence available to date indicates that microbial degradation may be a mechanism by which PCBs are eliminated from the environment (1,5,7,8,10,11,13). PCB resistant or degrading bacteria have been found to be ubiquitously distri buted in the natural environment (11). Thus far, degradation studies have centered on aerobic bacterial activity, and are not applicable, directly, to PCBs found in reducing or anaerobic sediments. Little information is available on the role of fungi. 1 HONS 013880 SPECIFIC AIMS 1. To isolate and cultivate fungi and anaerobic bacteria from a variety of freshwater, estuarine and marine environments which are able to grow in the presence of PCBs (Aroclor 1016 and Aroclor 1254) and to investigate their ability to degrade the PCBs. Initially, this will be done with mixed cultures; studies will be done with pure cultures as they are purified. 2. To determine the rate and mode of PCB degradation by the fungi and by the anaerobic bacteria in vitro; first with PCBs serving as the sole carbon source, and second with PCBs amended with alternate carbon sources and alternate inorganic energy sources. 3. To determine the rate and mode of PCB degradation by the fungi and by the anaerobic bacteria iri situ: natural deposits (with or without added PCBs) will subsequently be inoculated with a specific fungal or anaerobic bacterial culture, and the change in PCB content examined after incubation over pre-determined time intervals. Fungi and anaerobic bacteria will be added to sterilized sediments and non-sterilized sediments in order to investi gate the ability of the inoculated organisms to degrade PCBs under as natural conditions as possible. METHODS 1. Procedures for Specific Aim 1: Sediments for the isola tion of fungi and anaerobic bacteria will be collected"from 2 I MOMS 013881 representative sites in freshwater, estuarine and marine environments. These samples will be collected by the principal investigator or by requests of colleagues at other institutions. Physical and chemical aspects of the environment at the time of sampling (e.g., salinity, depth, pH, Eh, water temperature) will be recorded when possible. Immediately upon collection, the sediment will be transferred to duplicate sets of glass bottles which will be almost filled with filtered water and sealed with serum caps. One set will be gassed with nitrogen to maintain or to establish reducing conditions necessary for anaerobes; the other will be maintained aerobic for fungal isolation. Both sets of samples will be used for subsequent isolation of PCB degraders by enrichment culture. Enrichment cultures for anaerobic bacteria will be set up by adding 1-2 g of sediment to a 250 mi flask containing 100 ml of an organic medium (thioglycollate medium) made with distilled water or water of appropriate salinity and pH for estuarine and marine samples. Techniques for anaerobic cultures as described by Bryant (2) will be strictly followed. Cultures will be incubated for three days to two weeks at 26C; intermittently, culture flasks will be shaken by hand. Isolation will be carried out in a second flask which contains 100 ml of a mineral salt solution adjusted to the desired salinity: (NH4)2SC>4, 1 g; KH2P04, 0.2 g; K2HP04, 1.6 g; MgS04*7H20, 0.2 g; NaCl, 01. g; FeS04'7H20, 0.01 g; CaCl2'2H20, 0.02 g (pH 7.5) containing 0.05% of the appropriate PCB. Following standard anaerobic techniques, 1 ml of the enrich HONS 013882 3 ment culture will be transferred to this second medium and incubated as before until substantial growth is observed. Such cultures will be maintained in duplicate or triplicate. One series will be incubated in the light and one in the dark to enable the growth of phototrophic bacteria as well as strict chemotrophs and heterotrophs. Enrichment cultures for fungi will be started in Sabouraud dextrose agar (Difco) made to the desired salinity and incubated under aerobic conditions. After initial growth, 1 mi of the enrichment culture will be transferred to the PCB- raineral salts medium (described above) but amended with 1 mi of a trace elements solution per liter. The trace elements solution contains 0.1 g 0.05 g each of CuS04-5 HjO, MnSO^-HjO, ZnSO^7 HjO, Na2Mo04> and CoC12*6 H20 per liter. After incubation for predetermined time intervals (4 days to 1 month), aliquots of the incubated samples will be examined by high performance liquid chromatographic techniques, or they will be transferred to separatory funnels and extracted with hexane for gas chromatographic analysis. Gas chromato grams will be run with 1-5 vZ aliquots of the hexane extracts. Initially, only the disappearance of certain PCB homologs from the mixed culture systems will be measured. If the PCBs disappear or decrease in quantity, degradation products will be looked for, perhaps using thin-layer chromatographic techniques and with 14 C-labelled PCBs. Mixed cultures will be purified by plating onto mineral salts agar plates-containing 0.05t PCBs; degradation studies will be conducted with the resultant pure cultures. 4 MOMS 013883 2. Procedures for Specific Aim 2: Once the respective microbial systems capable of growth on PCBs are obtained, the kinetics of PCB degradation will be followed by analyzing the PCBs in the growth medium at different time intervals. The cells will be harvested by centrifugation and washed twice with 0.5 M phosphate buffer (pH 7.0). The washed cells will be injected into culture tubes containing the mineral salts medium and PCB. The procedures employed will be similar to those described by Halvorson et al. (6), who used resting suspensions of bacteria harvested from a sewage lagoon to examine the degradation of mixtures of pesticides. Comparisons will be made of gas'chromatograms of PCBs before and after incubation with test organisms and with non-inoculated PCB controls over the pre-determined time interval. Metabolites and residual PCBs will be characterized by the use of column and thin-layer chromatographic techniques of Stalling and Huckins (12), and Furukawa and Matsumura (5). 3. Procedures for Specific Aim 3: In situ studies will be conducted with natural sediments whose PCB content will have been determined previously. Sediments will be autoclaved to kill indigenous microbial flora. Depending on the test organ isms to be used in the test (fungi or anaerobic bacteria), the test conditions of the sediment will either be made anaerobic or left aerobic. The specific organism for the PCB degrada tion experiments will be inoculated into the sediments, and the rest of the experiment will be run as in Procedure fofSpecific Aim 2 to determine PCB degradation. A second variation of the 5 HONS 013884 in gitu study involves the inoculation of the test isolates into non-sterilized sediment of known PCB content along with all of its indigenous flora and fauna; this is designed to test the ability of the isolated PCB degraders to function and compete with other organisms under natural conditions. PCB degradation will be assessed as per Procedure for Specific Aim 2. 4. The research tasks will be performed in the following sequence, assuming a beginning date of June 1, 1979 June i, 1979 - May 31, 1980: Specific Aim 1. June i. 1980 - May 31, 1981: Specific Aim 2. June i. 1981 - May 31, 1982: Specific Aim 3. HONS 013885 6 LITERATURE REFERENCES 1. Ahmed, M. and D.D. Focht. (1973). "Degradation of poly chlorinated biphenyls by two species of Achromobacter." Can. J. Microbiol. 19: 47. 2. Bryant, M.P. (1972). "Commentary on the Hungate technique for culture of anaerobic bacteria," Amer. J. Clin. Nutrition 25: 1324. 3. Duke, T.W., J.I. Lowe, and A.I. Wilson. (1970). "PCB (Aroclor 1254) in the water, sediment, and biota in Escambia Bay, Florida," Bull Environ. Contamin. Toxicol. 5^: 171. 4. Fulk, R., D. Gruber, and R. Wullschleger. (1976). "Labora tory study of the release of pesticide and PCB materials to the water column during dredging and disposal operations," Final Rept. of U.S. Army Engineer Contract No. DACW39-74CoIT?. 5. Furukawa, K. and F. Matsumura. (1976). "Microbial meta bolism of polychlorinated biphenyl components by Alkaligenes sp.," J. Agri. Food Chem. 24: 251. 6. Halvorson, H., M. Ishaque, J. Solomon, and O.W. Grussendorf. (1971). "A biodegradability test for insecticides," Can. J. Microbiol. 17: 585. 7. Kaiser, K. and P. Wong. (1974). "Bacterial degradation of polychlorinated biphenyls. Identification of some metabolic products from Aroclor 1242," Bull. Environ. Contamin. Toxicol. 11: 291. 8. Liu, D. (1976). "PCB degradation," Mar Pollut. Bull. Ti 83. 9. Munson, T.O., H.D. Palmer, and S.M. Forns. (1976). "Trans port of chlorinated hydrocarbons in the Upper Chesapeake Bay," Nat'l Conf. on Polychlorinated Biphenyls, Chicago, 111., p. 2l8. 10. Sayler, G.S., M. Shon and R.R. Colwell. (1977). "Growth of an estuarine Pseudomonas sp. on polychlorinated biphenyl," J. Microbial Ecol. 3: 241. 11. Sayler, G.S., R. Thomas and R.R. Colwell. (1978). "Poly chlorinated biphenyl (PCB) degrading bacteria and PCB in estuarine and marine environments," Estuarine and Coastal Marine Science 6: 553. MOMS 013886 7 12. Stalling, D. and J. Huckins. (1973). "Reverse phase thin layer chromatography of some Aroclors, Halowaxes, and pesticides.," J.A.O.A.C. 56: 367. 13. Wong, P. and K. Kaiser. (1975). "Bacterial degradation of polychlorinated biphenyls II. Rate studies," Bull. Environ. Contamin. and Toxicol. 13: 249. 8 MOWS 01387 FACILITIES AVAILABLE The proposed research will be carried out using the facilities of the analytical chemistry and aquatic ecology sections of the Laboratory for Environmental Studies. The proposed research will be carried out in the culture and analytical facilities at the A.J. Lan2a Laboratory, Tuxedo, New York, and at the Hudson River field station maintained by the Medical Center at Buchanan, N.Y. NYU boats equipped for collection of samples and certain field analytical procedures are berthed at Stony Point, N.Y. and operate throughout the open-water months. Dock facilities are available at the Buchanan wet-lab, but only on a temporary basis. Equipment available for this project includes apparatus for: Extraction/concentration of organics Freeze-dry techniques Scintillation spectrometry (low level - beta) Gel electrophoresis Thin-layer chromatography ' Gas-liquid chromatography Respiration studies Water quality analysis Autoradiography Culture facilities and equipment for aerobic and anaerobic microorganisms Culture of aquatic organisms Maintenance of aquatic organisms . ... Computer data analysis HONS 013888 3 PRINCIPAL INVESTIGATOR ASSURANCE The undersigned agrees to accept responsibility for the scientific and technical conduct of the research project and for provision of required progress reports if a contract is awarded as a result of this application. Date Principal Investigator HONS 013889 PROPOSED BUDGET DEGRADATION OF POLYCHLORINATED BIPHENYLS BY FUNGI AND ANAEROBIC BACTERIA I. Personnel Name C.C. bee Title Prin. Invest. Theo. J. Kneip Tech. Advisor J.M. O'Connor Tech. Advisor Research Technician Laboratory Aide Clerical Assistance 1979--B0 % Time Salary 20 4, 592 10 N.C. 10 N.C. 100 12,500 50 5,071 10 1,200 Total Fringe benefits Overhead $23,363 4,731 12,559. Total salaries/benefits $40,653 Year 1980- 1981 % Time Salary 20 4,821 10 N.C. 10 N.C. 100 13,125 50 5,325 10 1,260 $24,531 5,029 12,879 $42,439 1981--82 % Time Salary 20 5,159 10 N.C. 10 N.C. 100 14,044 50 5,698 10 1,348 $26,249 5,447 13,780 $45,476 Equipment a. High performance liquid chromatograph (partial) b. Shaker table c. Incubator d. Spectrophotometer Totals $12,000 500 $12,500 - 600 1,150 $1,750 - - - ii HONS 0138B9*0 3. Supplies a. Glassware b. Chemicals c. Gases (N^; CC>2) d. Chromatographic equipment (gases, columns, etc.) 1979-00 $500 500 200 600 1,800 4. Miscellaneous Expenses a. Travel to scientific meeting b. Personal travel (collection, etc.) c. Publication costs d. Other costs (Xerox, inter-library loan, service, etc.) $500 500 - 500 Totals 1. Salaries 2. Equipment 3. Supplies 4. Miscellaneous $1,500 $40,653 12,500 1,800 1,500 Total amount requested $56,453 1980-81 5500 500 200 600 $1,800 $500 200 300 500 $1,500 $42,439 1,750 1,800 1,500 $47,489 1981-82 $300 200 400 $900 $500 100 300 300 $1,200 $45,476 900 1,200 $47,576 12 HONS 013891 CURRICULUM VITAE May 1979 Chun Chi Lee (Formerly Known as King Mon Tsue) Date and Place of Birth: Marital Status: Residence: Professional Address: Title: Education: Dissertation Title: Positions Held: Course offering: (Department of Environmental Medicine and Department of Biology) August 31, 1939 - Hong Kong Married, Two children R.D. #2, Felter Hill Rd. Monroe, New York 10950 New York University Medical Center Institute of Environmental Medicine 550 First Avenue New York, New York 10016 Telephone: (212) 679-3200, Ext. 5276 Research Assistant Professor Laboratory for Environmental Studies 1962 - B.S. in Zoology Colorado State University 1969 - Doctor of Philosophy in Marine Science University of Miami (Florida) The decomposition of organic matter in some shallow water calcareous sediments of Little Black Water Sound, Florida Bay New York University Medical Center Department of Environmental Medicine 1975 - Present Aquatic and Estuarine Microbiology University of Miami (Florida) Rosenstiel School of Marine and Atmospheric Science, 1969-1975 Research Scientist, Functional Biology 19-69- - 1973 Research Assistant Professor Biology and Living Resources 1973 - 1975 HONS 013892 Positions Held: Professional Societies: Research Interests: Awards and Grants: University of Miami (Florida! Institute of Marine Science 1962 - 1969 NDEA Fellow, Marine Biology 1962 - 1965 Graduate Assistant 1965 - 1967 Senior Field Biologist McMurdo Sound, Antarctica 1967 - 1968 Senior Field Biologist First International Weddell Sea Oceanographic Expedition 1968 - 1969 Colorado State University Department of Botany and Plant Pathology 1958 - 1962 Research Assistant, 1958 - 1962 American Association for the Advancement of Science American Society of Limnology and Oceanography Microbiology Algal and Protozoan Physiology and Ecology Biochemistry of Organic Matter in Aquatic Sediments Achievement Award in Biological Science Colorado State University, 1962 National Defense and Educational Act Predoctoral Fellowship, 1962 - 1965 National Science Foundation Grant University of Miami, $44,000 1973 - 1975 National Science Foundation Grant New York University Medical Center, $3,900 1976 HONS 013893 PUBLICATIONS C. C. LEE 1. Meyers, S. P., W. A. Feder and King Tsue Nutritional relation ships among certain filamentous fungi and a marine nematode. Science, 141, 520-522 (1963). 2. Meyers, S. P., W. A. Feder and King Tsue. Studies of relation ships among nematodes and filamentous fungi in the marine environment. Dev. Indus. Microbiol., 5., 354-364 (1964). 3. Altman,.J. and King Tsue. Changes in plant growth with chemicals used as soil fumigants. Plant Disease Reporter, 4, 600-602 (1965) 4. Bunt, J. S. and C. C. Lee. Observations within and beneath antarctic sea ice in McMurdo Sound and the Weddell Sea. Methods and Data. IMAS Special Publ., Univ. of Miami, 69-1 (1969). 5. Bunt, J. S. and C. C. Lee. Seasonal primary production within and beneath antarctic sea ice. J. Mar. Res., 2jH3), 304-320 (1970) 6. Bunt, J. S., K. E. Cooksey, M. Heeb, C. C. Lee and B. F. Taylor. Algal nitrogen fixation in the marine subtropics: Assay by acetylene reduction. Nature, 227, 1163 (1970) . 7. Corcoran, E. F. and C. C. Lee. The distribution of chlorinated hydrocarbon pesticides in the Mississippi River Delta and the adjacent Gulf of Mexico. Joint Southeast and Southwest ACS Meeting, New Orleans (Abst.). Dec. 2-4, 1970. p. 88. 8. Taylor, D. L. and C. C. Lee. A new Cryptomonad from Antarctica: Cryptomonas cryophila sp. Nov. Archiv fur Mikrobiol., 75, 269-280 9. Bunt, J. S., C. C. Lee and E. L. Lee. Primary productivity and related data from tropical and subtropical marine sediments. Mar. Biol., 16, 28-36 (1972). 10. Bunt, J. S. and C. C. Lee. Data on the composition and dark survival of four marine microalgae. Limnology and Oceanogr., 17, 458-461 (1972). 11. Fenchel, T. and C. C. Lee. Studies on ciliates associated with sea ico from Antarctica. I. The nature of the fauna. Archiv fur Protistenk., 114, 231-236 (1972). 12. Lee, C-C. and T. Fenchel. Studios on ciliates associated with sea ice from Antarctica. II. Temperature responses and tolerances in comparison with ciliates from temperate and tropical habitats. Archiv fur Protistenk., 114, 237-244 (1972). HONS 013894 PUBLICATIONS CONT'D. 13. Bunt, J. S., C. C. Lee and M. A. Heeb. The site off Freeport intended for Hydro-Lab. Hydro Lab J., 1^3-6 (1972). 14. Bunt, J. S., C. C. Lee. An exploratory study of benthic primary production. Hydro Lab J., 1_:7-10 (1972). 15. Taylor, B. F., C. C. Lee and J. S. Bunt. Nitrogen-fixation associated with the blue-green alga, Trichodesmium, as measured by the acetylene-reduction technique. Archiv fur Mikrobiologie, 88:205-212 (1973). 16. Lee, C.C., E. L. Lee and J. S. Bunt. Distribution of biomass in a coral reef transect. In: Results of the Tektite Program, coral reef invertebrates and plants (Sylvia Earle and Robert Lavenberg, editors), Natural History Museum, Los Angeles County, Science Bull., 2:69-75 (1975). 17. Steinhausler, F., S. Schaffer, N. Cohen, C. C. Lee, J. M. O'Connor and McDonald E. Wrenn. Effect of high LET radiation on intra cellular ATP content of prokaryotic and eukaryotic algae. Radi ation Research Society , 26th Meeting, Toronto, Canada (Abst.). May 14-18, 1978, pp. 591-592. 18. O'Connor, J. M., C C. Lee and M. Eisenbud. Environmental Health. MESA New York Bight Atlas Monograph 32. (In press). 19. Lee, C. C., R. Califano and R. Sansur. The degradation of poly chlorinated biphenyls (Aroclor 1254) by anaerobic bacteria and fungi from the Hudson River. Atlantic Estuarine Research Society, 61st Annual Meeting, Rehoboth, Del. April 26-28, 1979. MOMS 013095 Date and Place of Birth: Marital Status: Residence: Professional Address: Title: Education: Positions Held: CURRICULUM VITAE Theodore J. Kneip April, 1979 December 20, 1926; St. Paul, Minnesota Married, Six children 20 Stonefield Road Glen Rock, New Jersey 07452 New York University Medical Center Institute of Environmental Medicine 550 First Avenue New York, New York 10016 Telephone: (212) 679-3200, Ext. 5232 Research Professor, Deputy Director Laboratory for Environmental Studies 1950 - Bachelor of Chemistry (with distinction) . University of Minnesota 1952 - Master of Science University of Illinois 1954 - Doctor of Philosophy University of Illinois New York University Medical Center Department of Environmental Medicine 1967 - present . Senior Research Scientist 1967-1975 Adjunct Associate Professor 1969-1971 Adjunct Professor 1971-1975 Research Professor 1975 - present Acting Director, . Laboratory for Environmental Studies 1968-1970 Deputy Director, ' "* Laboratory for Environmental Studies 1970 - present HONS 013896 Curriculum Vitae Page 2 Course Offerings: (Department of Environmental Medicine) Analytical Chemistry of Environmental Contaminants Positions Held: (continued) Honor Societies: Professional Societies: Mallinckrodt Chemical Works St. Louis, Missouri 1954-1966 Research Chemist - Uranium Division 1957-1959 Group Leader, Uranium Division 1957-1959 Asst. Manager, Analytical Laboratory Uranium Division 1961-1963 Manager, Laboratory Products Research 1963-1966 Phi Lambda UpsiIon, 1950 Sigma Xi, 1954 American Chemical Society American Association for the Advancement of Science American Industrial Hygiene Association American Conference of Governmental Industrial Hygienists The New York Academy of Sciences Vice Chair - Environmental Sciences Section 1976 Chair - Environmental Sciences Section 1977-1978 Elected Fellow - 1977 Committees: Chairman of Subcommittee #6 - Metals 1 Intersociety Committee on Manual of Methods for Ambient Air Sampling and Analysis 1971-1977; Member 1971-present Member of International Meeting on Effects and Dose-Response Relationships of Toxic Metals, Tokyo, November 18-23, 1971. Organized by: The Subcommittee on Toxicology of Metals of the Permanent Commission and International Association on Occupational Ileal th. HONS 013897 Curriculum V1tae Page 3 Committees: (continued) Consulting: Member of American Society for Testing and Materials Committee 0-22: Methods of Sampling and Analysis of Atmosphere-., Subcommittees D-22.0T, 1973-present. 0-22.09, 1975-present. New York State Governor's ad hoc committee on PCO's, 1975. Secretariat, International Standard-. Organization/Technical Committee 14G (Air Qua 1ity)/Workinq Group 3 Nitrogen Compounds, 1975-1976. Secretariat, International Standards Organization/Technical Committee 2 Workplace Atmospheres, 1976-present. Various corporate organizations including Consolidated Edison, RSR Corporation, . Equitable Environmental Health. United States department of Commerce, National Oceanic and Atmospheric Administration, Marine Ecosystems Analysis Project, New York Bight Contaminant: Panel Cornell University, Hew York State College of Agriculture and Life Sciences and New York State College of Human Ecology, Cooperative Extension, New York City Urban Gardening Program. HONS 013898 Curriculum Vitae Page 4 List of Published Works: 1. Laitincn, H. A. and T. J. Kneip. "Polarographic and Coulonotric Study of p-Dirr.ethvlaninobonzcne," Journal of the American Chrnic ll Society, 7:73C-7-IO (1756). ' 1-------~ 2. King, P., E. Bretz and T. J, Kneip. "Determination of Iodine in Mineral Promixcs," Analytical Chemistry, _34: 563-567 (1962). 3. Kneip, T. J. , T. I!. Beaslv, P. King and W. K. Dean. "Programmed Temperature Gas Chromatography with Dual Electron Capture Detector for Pesticide Residue Analysis," Analytical C'nonistrv, 39:1510 1512 (1967). -- 4. Cohen, N. and T. J. Kneip. "A Method for the Analysis of 210Pb in Urine of Uranium Miners," Journal Health Physics, 17:125-130 (1969) . 5. Strehlow, C. D. and T. J. Kneip. "The Distribution of Lead and Zinc in the Human Skeleton," Journal American Industrial Hygiene Association, 20p;372-378 (1969). * Knein, T. J., G.'P. Howells and M. E. Wrenn. "Trace Metals, ' Radionuclides and Pesticides in the Hudson River," Svmposiun on Hudson River Ecology, October 1969, Proceedings of the Symposium, Hew York State Department of Environmental Conservation, Albany,' New York. ' 7. Furtado, V. C., T. J. Knein and M. Eisenbud. "Measurement of Low Levels of ^lj in Reactor Atmospheres," Nuclear Applications and Technology, 9: 268-273 (1970). .8 Knein, T. J., M. Eisenbud, C. D. Strehlow and P. C. Freudenthal. "Airoorne Particulates in Me/ York City," Journal Air Pollution Control Association, 2: 144-149 (1970). 9. Howells, G. P.. T. J. Knoip and M. Eisenbud. "Water Quality in Industrial Areas: Profile of a River," Environmental Science and Technology, 4y.26-35 (1970). 10. Kneip. T. J. "Atomic Atosrption Spectrophotometry," AIHA Confer ence on Advances in Instrumental Method of Analyses, Environmental Trace Substances Center, University of Missouri, Columbia, Missouri (May, 1970). HONS 0X3899 Curriculum Vitae Page 5 11. Laurer, Gi R- T. .7. Kneip R. E. Albert and F. 3. Kent. "In Situ Determination of Lead on Painted Surfaces fer the Prevention o Childhood Load Poisoning," Proceedings of the Third Svmoosiun on Low Energy X- and Gamma Rav Sources and Aon lica t ror.i;, Dos eon, Gordo end-Breach Science Publishers, New York, pp. 289-301 (i9 / i) . 12. Lcntsch, J. W., M. E. Wrenn, T. J. Kneip and M. Eiscnbud. "Manmade Radionuclides in the Hudson River Estuary," Proc. 5th Annual Midyear Topical Symposium, Health Physics Society, Idaho Falls, Idaho, p". 499 (197 0) . ' 13. Howells, G. P., K. Eisenbud ar.d T. J. Knein. "Ecology of the Estuary of the Lower Hudson River," Proc. FAQ Tech. Conf. on Marine Pollution and its Effects on Living Resources and Fishing, PuFH FIR:70/E-20, Food and Agriculture Organization or the United Nations, Hew York (1970). 14. Kneip. T, J. "Concentration Factors, Environmental Comolexity and the Analytical Chemist," American Chemical Society, Analytical Division--National Bureau of Standards Summer Symposium: Key to Progress in National Problem Areas, Washington, D. C. June 1971, Proceedings of the Svmnosium 15. Kneip. T J G. P. Howells and M. E. Wrenn. "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River," Proc. FAQ Tech. Conf. on Marine Pollution and its Effects on Living Resources and Fishing, Puhi. FIR:,'iP/7 0/E-20. Food and AgncuLturc organization of the United Nations, New York (1970). 16. Laurer, G. R., T. J. Kneip, R. E. Albert and F. S. Kent. "X-ray Flourcscence: Detection of Lead in Wall Paint," Science, 172 466-468 (1971). . -------------- ------ 17. Kneip, T. J., H. Cohen, D. H. Goldstein and E. A. 5. Kuchmore, "Experimental Studies of Lead Toxicity in Trace Substances in Environmental Health - V," Proceedinos of University of Missourij s 5th Annual Conference on Trace Substances In Environmental Health (ed. by D. D. Hemphill), pp. 349-361, ' ( June r57i). 18. Eiscnbud, M. and T. J. Kneip. "Trace Metals in the Atmosphere," Tech. Paper 116, New York State Department of Environmental Con servation, Environmental Quality Research and Development Unit (July, 1971). 19. Lentsch, J. W., T. J. Kneip, M. E. Wrenn, G. P. Howells and M. Eisenbud. "Stable Manganese and Manganese-54 Distributions in The Physical ar.d Eiolcgical Ccmconents of the Hudson River Estuary, Proc. Third National Svmr,oaiun on Radioecoloov, Oak Ridge (1971) . HONS 013900 Curriculum Vitae Page G 20. Kneip, T. J. "Sampling and Analytical Problems in a Complex -Natural System: The Hudson River Estuary," American Laboratory 3^47-52 (1971) . 21. Cohen, N., T. J. Kneip. D. Goldstein and E.A.S. Muchmore, "The Juvenile Baboon as a Model for Studies of Lead Poisoning in Children," Journal of Medical Primatology 1: 142-155 (1972). ~ 22. Kneip, T. J. and G. R. Laurer. "Isotope Excited X-ray Fluorescence, " Analytical Chemistry, 4_:57A-66A (1972). 23. Laurer, G., M. Eisenbud, R. E. Albert, T. J. Kneip and N. Nelson. "New York City Health Research Council' X-ray Fluorescence Analyzer for Lead," Isotopes and Radiation Technology 2:275-277 (1972). *24. Kneip T, J., et al., "Tentative Method of Analysis for Inorganic Lead Content of the Atmosphere," Health Laboratory Science 9 (1) :79 -- 83 (1972). 25. Laurer, G. R., R. E. Albert, T. J. Kneip, B. Pasternack, C. Strehlow, N. Nelson and F. S. Kent, "The Distribution of Lead Paint in New York City Tenement Buildings, AJPH 63 163-168 (1973). -------- 26. Kneip, T. J. and G. J. Lauer. ."Trace Metal Concentration Factors in Aquatic Ecosystems," in Vol. 5, Proqress in Analytical Chemistry, Plenum Press, New York") New York (1973) . 27. Ahuja, S., E. M. Cohen, T. J. Kneip, J. L. Lambert, G. Ziweig, eds. Chemical Analysis of the Environment and Other Modern Techniques, Plenum Press, New York, New York (1973) . 28. *29. Kleinman, M. T., T. J. Kneip and M. Eisenbud, "Meteorological Influences on Airborne Trace Metals and Suspended Particulates," Proceedings, 7th Annual Conference on Trace Substances in Environmental Health, University of Missouri, Columbia, Missouri, June 12-14 (1973) . Kncin. T. J.. et al., "Tentative Method of Analysis for Cadmium Content of Atmospheric Particulate Matter," Health Laboratory Science 10(3):214-217 (1973). *30. Kneip, T. J., et al., "Tentative Method of Analysis for Molybdenum Content of Atmospheric Particulate Matter by Atomic Absorption Spectrophotometry," Health Laboratory Science 10(3):218-220 (1973). ~ `Reprinted in Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, U.C. (1977) . HONS 013901 Curriculum Vitae Page 7 31. Kneip, T. J. , et al., "Tentative Method of Analysis for Nickel Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 1(3):221-225 (1973). 32. Kneip, T J, et al., "Tentative Method of Analysis for Copper Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(3):226-230 (1973). 33. Kneip, T. J., et al., "Tentative Method of Analysis for Chromium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 10(4) : 358-361 (1973) . 34. Kneip, T. J., G. P. Howells, M. E. Wrenn, "Trace Elements, Radionuclides and Pesticide Residues in the Hudson River," In: Marine Pollution and Sea Life. Fishing News (Books) Ltd., Surrey, England, p. 169 (1973) . (Reprinted from Ref. 15) 35. Kneip, T. J., M. T. Kleinman and M. Eisenbud, "Relative Contributions of Emission Sources to the Total Airborne Particulates in New York City," Proceedings, Third International Clean Air Congress, October 8-12^ TT73 (1974). 36. Cohen, N.,T. J. Kneip, V. Rulon and D. Goldstein, "Biological and Toxicological Response of Infant Baboons to Lead Driers in Paint," Env III th. Perspectives, Experimental Issue 117, DHEW PubTTTNIH 74-218, U.S. Gov't Printing Office, Washington, D.C., p. 161 (1974). 37. Kneip, T. J., N. Cohen and V. Rulon, "Potential Sources of Analytical Error in the Erythrocytic 6-Aminolevulinic Acid Dehydratase Test for Lead Exposure," Analytical Chemistry, 46:1863-1866, (1974). 3- Bernstein, D. M., T. J. Kneip, M. T. Kleinman, R. Riddick and M. Eisenbud, "Uptake and Distribution of Airborne Trace Metals in Man," Proceedings of the 8th Annual Conference of Trace Substances in Environmental Health, University of Missouri, Columbia, Missouri, June (1974) . 39. Kneip, T. J., G. Rfe and T. Hernandez, "Cadmium in an Aquatic Ecosystem: Distribution and Effects," Trace Substances in Environmental Health - VIT1, 1974 , A SymjiosTum. D. D. ilemphi 11, od . , University of Missouri, Columbia (1974). HONS 013902 Curriculum Vitae Page 8 40. nondietti, E. A., F. II. Swceton, T. Tamura, R. M. Porhac, L. D. Nulctt, and T. J. Kneip, "Characterization of Cadmium and Nickel Contaminated sediments from Foundry Cove, New York," I_n: Proceedings of the First Annual NSF-RANN Trace Contaminants Conference, Oak Ridge, Tennessee, Augun t, 1973 . pp. 211-224 . CONF-730802, ORNL, Oak Ridge, Tennessee (1974). *41. Kneip, T. J., et al., "Tentative Method of Analysis for Cadmium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 1_1 (2) : 112-116 (1974). *42. Kneip, T. J., et al., "Tentative Method of Analysis for Iron Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 118-121 (1974). *43. Kncip, T J, et al., "Tentative Method of Analysis for Lead Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 122-127 (1974). *44. Kneip, T. J., et al., "Tentative Method of Analysis for Manganese Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11 (2) :128-133 (1974). *45. Kneip, T. J-, et al., "Tentative Method of Analysis for Zinc Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(2): 134-139 (1974). . *46. Kncip, T J., et al., "Tentative Method of Analysis for Vanadium Content of Atmospheric Particulate Matter by Atomic Absorption Spectroscopy," Health Laboratory Science 11(31:240-245 (1974). *47. Knoip, T. J., et al. Tentative Method of Analysis for Elemental Mercury in Ambient Air by Collection on Silver Wool and Atomic Absorption Spectroscopy," Health Laboratory Science 11 (4) :342-346 (1974). MOMS 013903 Curriculum Vitae 1 Page 9 4B. Kneip, T. J., M. Eisenbud, M. T. Kleinman and D. M. Bernstein, "Studies of Trace Substances in an Urban Atmosphere," Proceedings of the International Symposium nf Recent Advances in the Assessment of the Health Effects of Environmental Pollution, Paris, June 24-28, 1974. Commission of European communities, Luxembourg, p.431-450. NTIS EUR53C0 (1975). *49. Goldstein, D. II., T. J. Kneip, V. Rulon and N. Cohen, "Erythrocytic 6-Aminolevulinic Acid Dehydratase (6-ALAD) Activity as a Biological Parameter for Determining Exposures to Lead," Journal of Occupational Medicine, 17_:157-162 (1975) 50. Kncip, T. J., "Potential Applications of X-ray Analytical ' Method in Paint Analysis," Journal of Paint Technology, 47:71-76 (1975). 51. Kneip, T. J., T. Hernandez and Gary He, "Cadmium in an Aquatic Ecosystem: Transport and Distribution," In: Trace Contaminants in the Environment," Proceedings of the Second. Annual NSF-RANN Trace Contaminants Conference, Asilomar, Pacific Grove, California, 29-31 August, 1974 TT?75) . 52 Kneip, T. J., et al., "Tentative Method of Analysis for Elemental Mercury in the Working Environment by Collection on Silver Wool and Atomic Absorption Spectroscopy," Health Laboratory Science 12(2) :158-162 (1975). 53. Kneip, T. J., et al., "Lead in Blood and Urine," Health Laboratory Science 12 (4) :375-378 (1975). 54. Kneip, T. J., et al., "Mercury in Urine," Health Laboratory Science 12 (4) :379--382 (1975). 55. Kneip, T, J., et al., "General Atomic Absorption Procedure rur Trace Metals in Airborne Material Collected on Membrane Filters," Health Laboratory Science 12 (4): 38 3-393 (1975). 56. Kneip, TJ, N. Cohen, V. Rulon, David Goldstein and N. Nelson, "Factors Influencing Dose-Response Relationships for Toxic Metals," In: Effects and Dose-Response Relationships of Toxic Metals, G.F. Hordborg, ed.. Associated Scientific Publishers, Amsterdam (1976). 57. Eisenbud, M., T. J. Kneip, M. T. Kleinman, D. M. Bernstein, "Trace Metals in Urban Aerosols," Final Report to Electric Tower Research Institute, October, 1975. NTIS Pb-248-324 (1976). HONS 013904 Curriculum Vitae Pa<jQ 10 58. Bernstein., D. M., M. T. Kleinman, T. J. Kneip, t. L. Chan and M. Lippmann, "A High-Volume Sampler ior the Determination of Particle Size Distributions in Ambient Air,*' Journal of the American Air Pollution Control Association 26(11), m9^ro7rrr57?'). 59- Kleinman, M. T., T. J. Kneip and M. Eisenbud, "Seasonal Patterns of Airborne Particulate Concentrations in New York City," Atmospheric Environment 10 : 9-11 (1 9 7 G) . 60 Kleinman, M. T., D. M. Bernstein and T. J. Kneip, "An Apparent Effect of the Oil Embargo on Total Suspended Particulate Matter and Vanadium of New York City Air," J. Air Poll. Con. Assoc. 2^7:65-67 (1977). 61. Kneip, T.J., R. Peter Mallon and Virginia P. Rulon, "The Effects of Anemia on Heme Synthesis Parameters During Load Exposure," American Industrial Uyqiene Association Journal 37, 578 -585 (1977) . 62. Kneip, T. J., et al. , "Analytical Method for Antimony In Air and Urine," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 7C5-769 (1977). 63. Kneip, T. J., et al., "Analytical Method for Arsenic In Urine and Air," In: Methods of Air Sampling and Analysis, Second edition, Katz, M. , ed. American Public Health Association, Washington, D. C., pp. 769-773 (1977). 64. Kneip, T. J., et al., "Analytical Method for Arsenic, Selenium and Antimony in Urine and Air by Hydride Generation and Atomic Absorption Spectroscopy," In: Methods of Air Sampling and Analysis, Second edition, Katz, M., ed. American Public Health Association, Washington, D. C., pp. 774-780 (1977). 65. Kneip, T. J., et al., "Analytical Method for Chromic Acid Mist in Air," In: Methods of Air Sampling and Analysis, Second edi tion, Katz, M. , ed. American Public Health Association, Washington, D. C., pp. 785-788 (1977). 66. Kneip, T. J., et al., "Analytical Method for Load in Air," In: McthoJs of Air Sampling and Analysis, Second edition, Katz, M., cell American Public Heal th Association, Washington, D. C., pp. 808-818 (1977). 67. Hazen, R. E. and T. J. Kneip, "The Distribution.-pf Cadmium in the Sediments of Foundry Cove," In: Hudson River Ecology, Proceeding of a Symposium, Hudson River Environmental Society! ed. March 197G (1977). HONS 013905 Curriculum Vitae Page 11 68. Kneip, T. J., M. T. Kleinman, R. Riddick and D. M. Bernstein, "Trace Elements in Human Tissues," Proceedings of the International Workshop on Biological Specimen Collections, April 1 U-22, i 9 7 7 , Luxembourg, (in press, 1977) . 69. Kneip, T. J., "Effects of Cadmium in an Aquatic Environment," In.: Cadmium 77, Edited Proceedings First International Cadmium Conference, San Francisco, 31 Jan.-2 Teb.. 1977 . Metal Bulletin Limited, London, [Tp"! 120-124, (1978). 70. Daisey, J. M. , I. ilawryluk, T. J. Kneip and F. Hukai, "Mutagenic Activity in Organic Fractions of Airborne Particulate Matter," Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 71. Kneip, T. J., M. A. Leyko, M. T. Kleinman, M. Lippmann and J. M. Daisey, "Organic Matter in New York City TSP: Comparisons of Seasonal Variations and Relationships to Source Tracers," Presented at the Carbonaceous Particles in the Atmosphere Conference, March 20-24, 1978, Berkeley, California. 72. I.ioy, P. J., G. T. Wolff and T. J. Kneip, "Toxic Airborne Elements in the New York Metropolitan Area," J. Air Poll. Con. Assn. , 2(5):510-512 (1978). 73. Leaderer, B. P., D. M. Bernstein, J. M. Daisey, M. T, Kleinman, T. J. Kneip, E. 0. Knutson, M. Lippmann, P. J. Lioy, K. A. Rahn, D. Sinclair, R. L. Tanner, and C. T. Wolff, "Summary of the New York Summer Aerosol Study (NYSAS)," J. Air Poll. Con. Assn., 28(4):321-327 (1978). 74. Fennikoh, K. B., H. I. Hirshfield and T. J. Kneip, "Cadmium Toxicity in Planktonic Organisms of a Freshwater Web," Environ. Res. June (1978) . 75. Kneip, T. J. and M. T. Kleinman, "Sampling and Multielement Analysis of Ambient Aerosols," In: Special Environmental Report No. 10, Air Pollution Measurement Techniques, Proceedings of the WMO Air Pollution Measurement Techniques Conference (APOMET), Gothenburg, 11-15 October, 1976. World Meteorological Organization, Geneva, Switzerland, pp.132-139, (1977). 76. Lnurcr, G. R. and T. J. Kneip, "Portable X-ray Units for Analyzing Lead in Paint," Iji: A Report of the Committee on Environmental Improvement: Assessing iho Problem of Lead-Based Paint Hazards, pp. 13-17, American chemical Society, Washington, D.C. (August, 1978). HONS 013^06 Curriculum Vitae Pago 12 77. Kneip, T. J. and R. E. Hazen, "Deposit and Mobility of Cadmium in a Marsh-Cove Ecosystem and the Relation to Cadmium Concentration in Biota." Environ. Health Persp. Feb. (1979) . 78. Lowenberg, R. and T. J. Kneip. "Dietary intakes of lead and cadmium in vegetables grown in New York City." Environ. Health Persp. (In press, 1979). 79. Kneip, T. J. "Concentrations of lead and cadmium in garden vegetables grown in New York City." Environ. Health Persp (In press, 1979) . 80. Ilazen, R. E. and T. J. Kneip. "Biogeochemical cycling of cadmium in Marsh Ecosystem." In: Bioqeochemistry of Cadmium, Wiley and Sons, New York. J. O. Nriagu, Ed. (In press, 1979) . 81. Daisoy, J. M., M. A. Leyko and T. J. Kneip, "Source identification and allocation of PAH compounds in the New York City aerosol: Methods and applications." In: Polynuclear Aromatic Hydrocarbons, p. w. Jones and P. Lever, eds. Ann Arbor Science Publishers, Inc. Ann Arbor, Michigan (In press, 1979) pp. 201-215. 82. Kneip, T. J., M. Lippmann, F. Mukai and J. M. Daisey, Trace Organic Compounds in the New York City Atmosphere. Part I. Preliminary Studies. Report to Electric Power Research Institute. (In press, 1979). 83. Kneip, T. J. and M. Lippmann, Eds. New York Summer Aerosol Study. Annals of the New York Academy ot Sciences. (In press, 1979). 84. Kneip, T. J. and P. J. Lioy, Eds. Aerosols: Anthropogenic and Natural--Sources and Transport. Annals of the New York Academy of Sciences. (In press, 1979). 85. Kneip, T. J., Reviews on 1) Haloethern and Haloalkylothers; 2) Phthlalate Esters; 3) Nitrosamines; 4) Dichlorobenzidine. In: Chemical Pollutants of New York Bight: Priorities, J. S. O'Conner and 11. M. Stanford, eds. NOAA/MESA Report (In press, 1979). HONS 013907 PERSONAL EDUCATION HONORS AWARDS SOCIETIES AFFLILIATIONS CURRICULUM VITAE JOSEPH MARTIN O'CONNOR Born: November 19, 1944, Boston, Massachusetts Married: 2 children Bachelor of Science, Biology June, 1966 College of the Holy Cross Worcester, Massachusetts (Philosophy minor) Doctor of Philosophy, Ecology/Physiology November, 1971 State University of New York at Albany Albany, New York Dissertation Title: Photoperiodic control of pituitary gonadotropin release in trout (Salmo qairdneri Richardson and Salvelinus fontinalis (Mitchell) and in the leopard froq (Rana pipiens Shreber) Sigma Xi Beta Beta Beta Biological Society Student representative to Faculty Senate State University of New York at Albany R.E.S.A. (Sigma Xi) Research Grant-in-Aid N.S.F. Pre-doctoral fellowship (Honorable) Research Grant-in-Aid, Edmund Niles Hyuck Preserve Fellowship, Research Foundation of New York State New York Academy of Sciences American Society of Limnology and Oceanography Estuarine Research Federation Atlantic Estuarine Research Society AAAS American Society of Natural History Ecological Society of America American Fisheries Society Sigma Xi Great Lakes Research Society Florida Defenders of the Environment Board of Consultants, Lawler, Matusky and Skelly Engineers, Tappan, New York HONS 013908 EXPERIENCE present 1974-75 1973-74 1970-73 1967-70 1965-67 (summers) PROFESSIONAL ACTIVITIES 2 Assistant Director Laboratory for Environmental Studies Institute of Environmental Medicine New York University Sterling Forest, Tuxedo, New York Senior Biologist Office of Professional and Technological Development Lawler, Matusky and skelly Engineers 415 Rt. 303, Tappan, New York Director Biological Studies Section and Laboratory Office of Research and Technology Quirk, Lawler and Matusky Engineers and QLM Laboratories, Inc. Tappan, New York Research Associate University of Maryland Natural Resources Institute Hallowing Point Field Station Prince Frederick, Maryland Graduate Research Fellow Dept, of Biological Sciences State University of New York at Albany Faculty Research Assistant University of Maryland Natural Resources Institute Chesapeake Biological Laboratory Solomons, Maryland Member, Advisory Committee Chesapeake Bay R.A.N.N. Program of Science Foundation 1971-1972 the National U.S. Dept, of Agriculture Graduate School Programs in Aquatic Ecology and Pollution Biology Counselor Rockland County Guidance Center for Women Guidance in Career Programs Adjunct Assistant Professor Inst, of Mar. and Atmos. Sciences of the City University of New York HONS 013909 Advisor, Fisheries Biology u.S. Dept, of Commerce National Oceanic and Atmospheric Administration New England Offshore Mining Environmental Study 1972-1974 Visiting Investigator University of Delaware College of Marine Studies Bayside Laboratory Lewes, Delaware 1972 RESEARCH INTERESTS' Population dynamics Community dynamics Respiratory physiology of fishes Reproductive physiology of fishes Chemical-biological interrelationships in ecology Ethology of fishes TEACHING INTERESTS Physiological ecology General physiology General ecology Pollution biology Ichthyology PUBLICATIONS O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of frog ovarian oocytes. Amer. Zool. 9:549. O'Connor, J.M. and R.G. Stross. 1970. Photoperiodic control of the pituitary gonadotropin release patter of the frog, Rana pipiens Shreber. Abstr. Proc. N.E. Division Comp. Physiology section, Amer. Soc. Zool. O'Connor, J.M. 1970. The effects of thermal shock on the tidal activity patterns of the hogchoker, Trincctes maculates. Abstr. Amer. Society of Ichthyologists and Herpetologists, Ann. Mtg., New Orleans, La. O'Connor, J.M. 1971. Photoperiodic control of the pituitary gonadotropin release patterns in the rainbow trout (Salmo qairdneri (Rich.,)), the brook trout (Salvelinus fontinalis Mitchell) and in the leopard frog (Rana pipiens Shreber). Ph.D. Thesis. Library, State University of New York at Albany, and University Microfilms, Ann Arbor, Mich. MONS 013910 4 O'Connor, J.M. 1972. Pituitary gonadotropin release patterns in rainbow trout (Salmo gairdneri) brook trout (Salvelinus fontinalis) and in the leopard frog (Ra na pipiens). Comp. Biochem. Physiol. 43(A):739-746. O'Connor, J.M. 1972. Tidal rhythm in the activity patterns of the hogchokcr, Trinectes maculatus (Bloch and Schneider). Exp. Mar. Biol. Ecol. 9:173-178. Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1972. The effects of suspended and deposited sediments on estuarine organisms. Univ. Md. N.R.I. ref. 72-9. Sherk, J.A. , J.M. O'Connor and D.A. Neumann. 1973. The effects of suspended and deposited sediments .on estuarine organisms. Univ. Md. N.R.I. ref. no. 73-4. O'Connor, J.M., R.W. Williams and J. Converse. 1974. Studies of fish impingement at the Nine Mile Point Nuclear Station, Lake Ontario. Abstr. 17th Conference on Great Lakes Re search. Int'l Assn, for Great Lakes Research. O'Connor, J.M. and J.A. Sherk. 1974. The responses of some estuarine organisms to Concentrations of suspended solids. Proc. of the Seventh Dredging Seminar, Ctr. for Dredging Studies, Texas A&M University. Sherk, J.A., J.M. O'Connor and D. Neumann. 1975. The effects of suspended sediments on estuarine environments. IN: L.E. Cronin (Ed.). Estuarine Research, Vol. II. Geology and Engineering, Academic Press, N.Y. pp. 541-557. Neumann, D.A., J.M. O'Connor and J.A. Sherk. 1975. Respiratory and hematological responses of the oyster toadfish (opsanus tau) to suspended sediments. Trans. Amer. Fish. Soc. ToT:77 5-781. Sherk, J.A. and J.M. O'Connor. 1976. Effects of sedimentation on coastal zone organisms. Proc. Ill Federal Inter-Agency Sedimentation Conference. O'Connor, J.M. and S. Schaffer. 1977. gear on striped bass ichthyoplankton. 18:312-315. Effects of sampling Chesapeake Science Sherk, J.A., J.M. O'Connor and D.A. Neumann. 1976. Effects of suspended solids on selected estuarine plankton. U.S. Army Corps of Engineers Coastal Engineering Research Center. Misc. Rept. 76-1. 50 pp. Jan. 1976. MOMS 013911 O'Connor, J.M., D.A. Neumann, and J.A. Sherk, Jr. 1976. Lethal effects of suspended sediments on estuarine fish. Technical Paper No. 76-20, U.S. Army, Coastal Engineering Research Center, 38 pp. O'Connor, J.M. , D.A. Neumann, and J.A. Sherk. 1977. Sublethal effects of suspended sediments on estuarine fish. Technical Paper No. 77-3, U.S. Army, Coastal Engineering Research Center, 90 pp. Rachlin, J., A.P. Beck, and J.M. O'Connor. 1978. Karyotypic analysis of Hudson River striped bass, Morone saxatilis (Walbaum) . Copeia 1978 ( 2) :334-336 . Ginn, T.C. and J.M. O'Connor. 1978. Responses of the estu arine amphipod Cammnrus daiberi to chlorinated power plant effluent. Estuarine Coastal Mar. Sci. 6:459-469. Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1978. An opera tional system for simulating the physical and chemical en vironment of the condenser tubes of a power plant. IN: Thorp, J. and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic systems (in press). Zubarik, L.S., and J.M. O'Connor. 1978. A radioisotopic study of mercury uptake by Hudson River biota. IN: Thorp, and J.W. Gibbons, eds. Energy and Environmental Stress in Aquatic Systems (in press). J. O'Connor, J.M., C.C. Lee, and M. Eisenbud. Environmental Health. M.E.S.A. Now York Bight Atlas Monograph 32 (in press). Ginn, T.C., G.V. Poje, and J.M. O'Connor. 1978. Survival of planktonic organisms following passage through a simu lated power plant condenser tube. Proc. Fourth Nat'l Workshop on Entrainment and Impingement, pp. 91-101. PUBLICATIONS IN REVIEW O'Connor, J.M., D.A. Neumann and J.A. Sherk. The responses of estuarine fishes to highly turbid environments. Water Res. O'Connor, J.M. and J.A. Sherk. The effects of suspended mineral solids on the white perch, Morone americana. Trans. Amer. Fish. Soc. O'Connor, J.M. and J.A. Mihursky. The effects of thermal shock on the activity patterns of the hogchoker, Trinoctcs maculatus. Chcs. Sci. HONS 013912 6 MANUSCRIPTS IN PREPARATION Eichorn, K.L., T.C. Ginn and J.M. O'Connor. Response of striped bass (Morone saxatilis) to chlorinated cooling water. O'Connor, J.M., G.V. Pojo, and S. Rigdon. Respiration of striped bass (Morone saxatilis) eggs and larvae. Becker, A.J. and J.M. O'Connor. Aerobic shutdown in the green crab (Carcinus maenas) in response to temperature, salinity and sulfide. Steinhausler, F., S. Schaffer, N. Cohen, C. Lee, J. O'Connor and M. Nrcnn, Effect of high LET radiation on intracellular ATP Content of prokaryotic and eukaryotic algae. Hendrick, E., J.M. O'Connor, and G.R. Laurer. A method for analysis of trace mineral content of fish otoliths using X-ray fluorescence spectroscopy. PRESENTATIONS . O'Connor, J.M. 1969. The effects of melatonin on in vitro ovulation of ovarian oocytes in the frog, Rana pipiens. Amer. Soc. Zool. O'Connor, J.M. and R.G. Stross. 1969. Photoperiodic control of the pattern of pituitary gonadotropin release in the frog Rana pipiens Shreber. Amer. Soc. Zool. O'Connor, J.M. 1966. Alterations in the tidal activity rhythm in the hogchoker after exposure to thermal shock. Atlantic Estuarine Research Society, Cambridge, Md. October, 1966. . O'Connor, J.M. and J.A. Sherk. 1971. The lethal effects of suspended solids on estuarine fishes. Joint Mtg. Atlantic Estuarine Research Society and the Estuarine Research Federation. Pleasantville, N.X. October, 1971. Neumann, D.A., J.M. O'Connor and J. Sherk. 1972. Studies of the respiration of the striped bass and the white perch exposed to suspended sediments. Atlantic Estuarine Research Soc. Ocean City, Md. April, 1972. O'Connor, J.M., J.A. Sherk and D. Neumann. 1973. "Inert" mineral compounds as potential factors in mortality and toxin transport in estuarine organisms. E.P.A. v.'orkshop on Chemical Pathogens in the Environment. Pensacola, Fla., May, 197 3. .... MOWS 013913 7 Sherk, J.A., J.M. O'Connor and D. Neumann. 1973. The effects of suspended solids on estuarine environments. Symp. on Recent Advances in Estuarine Research. IX int'l. Conference Of the Estuarine Research Federation. Myrtle Beach, S.C. October, 1973. O'Connor, J.M., and J.A. Sherk. 1974. The responses of some estuarine organisms to concentrations of suspended solids. Seventh Dredging Seminar. Sp. by the Center for Dredging Studies, Texas A&M University, New Orleans, La. November, 1974. O'Connor, J.M., R. Williams and J. Converse. 1974. Impinge ment studies at the nine mile point nuclear power station, Lake Ontario. 17th Conference on Great Lakes Research, August, 1974. Hamilton, Ontario, Canada. O'Connor, J.M., and S.G. O'Connor. 1975. Seasonal Compartmentalization of primary productivity in the New York Bight, 1973-74. Am. Soc. of Limnology and Oceanography, Halifax, N.S., Canada, June, 1975. O'Connor, J.M. 1976. Assessment of entrainment impact at the Indian Point Nuclear "Station. Third National Work shop on Entrainment and Impingement, New York City. Poje, G.V. and J.M. O'Connor. 1976. Gammarus tigrinus: an estuarine predatory carnivore. Presented to the Atlantic Estuarine Research Society. May 1976. Ginn, T.C., K.L. Eichorn and J.M. O'Connor. 1976. Response of the striped bass Morone saxatilis and the amphipod Gammarus to chlorinated power plant effluent. Northeast Fish and wild life Conference. April 26-29, Hershey, Pennsylvania. Poje, G.v., T.C. Ginn and J.M. O'Connor. 1977. Use of a condenser tube simulator for evaluating power plant entrain ment effects. Northeast Fish and Wildlife Conference, Boston, Massachusetts. April 3-6, 1977. Poje, G.V., T.C. Ginn, and J.M. O'Connor. 1977. An operational system for simulating the physical and chemical environment of the condenser tubes of a power plant. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. Zubarik, L.S., and J.M. O'Connor. 1977. A radioisotopic study of mercury uptake by Hudson River biota. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. MQNS 013914 8 O'Connor, J.M..C.C. Coutant, and J.p. Lawler. 1977. Environ mental impact assessment as ecological research. Symposium on Energy and Environmental Stress in Aquatic Systems, Augusta, Georgia, November, 1977. O'Connor, J.M., T.C. Ginn, and G.V. Pojc. 1978. Survival of planktonic organisms following passage through a simulated power plant condenser tube. Fourth Nat'l Workshop on En trainment and Impingement. Chicago, Illinois. Steinhausler, F., S. Schaffer, N. Cohen, C. Lee, J. O'Connor, and M. Wrenn. 1978. Effect of high LET radiation on intra cellular ATP content of prokaryotic and eukaryotic algae. Radiation Research Society. Toronto, Ontario, Canada. HONS 013915