Document 0JomQ1oj94xjRnED4p8beLKQb
off 3M
SKatheerinee. Rneet POD.
SIeMeEsavOipreorsnimoemnsal Hoskth 31d
AR326_ IHG
9o00a8Fas,t
Ave, Bung 5135-331
235.26
681778 4331
nwzoos THQDl0S 00373 884476 Corti Mi
nog 03EE nny
mE 2 --_ %
Oc ofaon Fm Tov RDoocoumme6n4t26Processing Center (Mail Code 7407M)
NO TSCA CBI
Bedp-4o-27
Attention: TSCA 8(e) Coordinator
c
-
-
US. Environmental Protection Agency
Cont
W1a2s0h1iCnogntsotni,tuDt.ioCn.Av2e0n4u6e0, N. W.
Ntaaiin NO cg,
Re: FTlSuCorAocSheecmtiicoanls8,(eD)oScukpeptleNmose.ntal Notice: Sulfonate-based and Carboxylic-based
Dear Sir:
u3oMroischsuebmmiictatilnsg ths notice to supplement its previous submissions on sulfonyl and carboxylic-based
Hethure Fl z= 3M has recently receivedthe enclosed analytical report whichpresentsdata from 36 lots of
`commercially available normal pooled human serum that were purchasbeyd the 3M Environmental
Laboratory and subsequently screened for endogenous fluorochemicals. Each: sample was analyzed
tupuhesseriifnnrlggeuplaooirrpqotruowoictdatecsaihnndrepeorsrmeuiaclvtifepoodigntarfatartepiooh(mnyPeFttxeOtacSrnha)dnpieo(qmlCuayetmFeaa1dsn,csSduOsrsypvc)eercesatenarndonemdpdeefstrohrfroyluus(loiLdxrtCobeo/eecMntcSado/ninoMfsifSice)drae.ecrnietMddfu(lacuPohFsroOeofrcAeht)eehnm(eiiCncd;gaaFltq,sau,siapClnriOcetOlsyHued)dntai,enadgin
sconehlneyemraainldcyanacolrotersrnoeobcroerwsasetarirevielpyroofrpderpuercveesideonubtsyalt3iyvMer.eopfoItrnhtaeedddgivetanileournea,sltpahoenpdtuoelvraetldisooonsfollteuvreoelrspo.rcehsNeeonmttica"lasllousfbteshpaeotradielotdeTcstkreed
information
=
1Fyouhave any questions, plese dono esa1 ctonteact Dale L. Baconat 651) 784736. 22 _23
Sincerely,
i) is
= 25
w o2
SKtaa tVhiecrienDPe.reRseiededn,tPhD
a
Environmental Technology and Safety Services
BEST COPY AVAILABLE (nn
e------------e -- Co h
-_--_--m--m--m--m--mm--m---- sw EROMEeNTARL LeUsOERAoTOnRY
3M
NO TSCA CBI
Analytical Report
Revised August 12, 2005
Initial Quantitative Obtained in 2004
fSocrrEeenndionggeonfouCosmFmleurocrioaclheLmoitcsalosf
HUsuimnagnPrSoetreiunm
Precipitation and Liquid Chromatography/Tandem Mass Spectrometry
l eee em Laboratoi ry Reques-- t Nruemebrere:-- rEs05-t012-- 0bee-- en
Testing Laboratory
eee3M--Envi--ro3nM--meEnntv--ailroH--nemaelnt--htaaln--LdabSoa--rfaettyo--rOype--rati--ons ------
.
3M Environmental Laboratory
Project Lead Mark E. Ellefson
Building 2-36-09" PO Box 3331"
Saint Paul, MN 55133-3331
Phone: 651-778-5405
Testing Facility Management
Wiliam K. Reagen, Ph.D.
Building 2-3E-09
PO Box 3331 Saint Paul, MN 55133-3331
Phone: 651-778-6565
Sr o me--------------------------
Requestor
Dale Bacon
Building 2-3E-09 PO Box 3331
Saint Paul, MN 55133-3331 Phone: 651-778-4736
Page tors 2
.+ 0EVRONMERNTAE LLANBGSOERoAsTT 0O1R2Y0 3MEnvironmentalLaboratory Analytical Report ProjectNumber: E05-0120
Tpohoelefdohlluomwianngsanearluytmitchaaltrweeporretppruerscehnatsseddabtyafthreom3cMoEmnmveirrcoinamlelnytaavaliLlaabbolreantoorrmyaalnd subsequentlyscreened forendogenous fuorochemicals. Tphriortteyi-nspirxesceippiatraattieolnottsecohfnpioqouleaednndoscrrmeaelnheudfmoarnssiexrteuemn wdeirffeeerxetnrtfacltueodruoschienmgicaals uSsaimnpglleisquwiedrcerehceriveodfmroam vtarioougssmurappsalsiseppresbchettryowme/eetnrt9y/(a9L/0Cn4/aMdnSd/eM1S2m)/.2/0S4e.ruSmamples. were analyzed between 02/03/05 and 02/09/05. (L"TLhiOsQrsan)goe-ffainpdipngsrtuodyxwia1sm0d0ae-st3ig0en0elpdptyto.prUopvoindreelvoiweewrolfimtihtesodaftaq,uainttwiatastdiiosncovered 1t0ha0t-m3a0n0ypofptttheatarrggleeetvaetnla.lyAtsesanwienrteeprrimesseoaltuattiocnoanncednutnrtaitlioanmsomruecdhelfionwietirvtehsatnutdhye (LanObDe)cwoamspdleeftiende,dsfiognraelatcohnaoniasleytrea.tiAosswaerreeseuvlatl,ucataeldaindbarcluiarmvitetsoiwfeodrenteection equxatlriatpyodlaatteatdboebtewdeeetnetrhmeinLeLdOfoQrasnodmtehe=n~aelwytleysdaesfilnoewdaLsO1D9,.9aplplto.wiTnhgsecrreefeoariient,g e`xmtursatpboelaetmepdhcausrivzeesdtahnadstmhouuclhdobfetchoendsai:deprreedsaesnstcerdeienntihnigsrqueaploitrytwdaatsadeornliyv.ed from s`aSmapmplleesriensuwlthsiacrhethseruewmamsdaetreicnitTaabzlbeleaen1da.lyTthe.eSaavemrpalgeevvaalluueesstrheaptowretredereepsrsetsheaantthe limitofdetection werenotincludedintheaveragevaluecalculations.Pleaserefer to Section 5forindivsaimpdleureasulls.
Page2018 5 i ----------------------------------
_--_--
Table 1. SumofmPefaluorrinayted Acid Results summRoNENTRAeLeAsOeRATrORY
| wen so[es[ow [oman|128|owt[osm col P E EomeA rI T-
EET FT
ETEeAN SHRE SESRR
Vo g |
[ [Mt edoiann tow
T17o75s05o85][|5276455s |||WnWRRe"[[|0o19e08s24s[[|24a8r50r|]|7o2s66o5r|||wwnawe [[oossseeot[[ei<iooonn]]
|_StndardDeviation |2.17 |067 |NR [oss]5.64[ori|ww Tozos | no"|
Nuom fSab mple es wr ith
DNetDeTctNaobtleDAeneatleydt.eTh ssvosded oy 702 opi
aly NR NotR?epor= tepd.eThilsanbaelyetsewasdetected iarseeofith]e365srampelersbutappeaErsetobe 3 cdoyn:taminantor
Tabl2e. SummaryofOtherPafucrinatedand HighlyFluorinatedAnalytaResults
[ooml | wan[a0e [sen [sem n aos|sos| [M fF eMw edin -- }|<<o0ooonoloonos}ms3e2rs7|||1166o 30||cc<ooeob.Js|o0os0s8t|m||
[Wh Too 0.190 [00ros| 580 | 328| 00 | 0.185|
tow
[StanFdraarodfcSaDtemvpiilaetsoiwoinnth
T| N<o"oJo00s1l 2[0o012o2]10s.758210|2167.11o1||c<N2 o0]1|n000 o2s5ef7||
VD NotDette.Thi a asmodecd nyoe pie
NMR data. Peaks were observed in thechromatogramscorresponding to linearand branched
idseotmeerrmsinfeodrfPoFrOboStahnadnaPlFytOeAs.aRnedlaarteipvreesberaatnecdhbeedlaonwd(ltinheeahruimsaomnesrerrautimovsawleuree.
represents anaverageof %branchedisomers forallof the humanserumsamplesthat
containeddetectable levels of eachanalyte). "The %branchedisomer results for
individual samarpe pl rese ents ed in Table
PFOS(Lot171)and PFOA(Lot 332)were
1a2n.aIlnyzaedddittoigoent,hewrealls-cahmaartacrtiexrsipziekdel
ots
of
(QC05020in3ra-bb0it0se4ru)m. Data fromthatanalysis wasevaluatedfor
`branched andlinearisomerdistributionandagreeswellwithpreviously obtained
Page 3aris 4
_-- sw ewmOMEi NTALr LAsORk ATORY
Table3.Summaryof PFOAandPFOS isomerRatios
E RE e = ETERS
Ee
Ea
CRE TR
| |WH __autm ixasn oiSke e(u | |am c2o6a %%sv o| | zeoa ss30g w%0eq| |
Ses Tw L6252(NMRchiractarzad
ng|_|on] Lot 171(NMRcharacterviazleued
EERE
ECRRSMEY
Tabl4e. ReferenceSubstances
Lse L DR te Siprlsaa h0)ER oa de (SG. CEE| T toaec ]
| vatanboinds |pacino | wa |rom |wax[om |
[ontop a | accoace | awe |ronmaworor| sesw| eos |
[reuters | a Goact |otis| tren |oe| om |
[Tribecd | iA pc | wwe | toa | maw[eon |
|retuooscnnenst |sroncincd| ow ___|rovimoowoin [mow| oom |
[omietommmicnit| nat |ouvir | tan |mm| om |
([oombtomiomotoa| Clorc |ours| tons |sox| oom |
|roto
s |cnc |ouverts| tran | seen| oon |
[remote s | caso | umd | torsrioun |mem| eon |
[rotommentoomie | ms | ow __| rose[moon| 07 |
[rotons| voix|
[formo [osma|
[Cette | ome| ow | romomian| nw| ows |
[rEtmEE
HE
oworss| | svwwi |[towrs [mwaenra || oon wn|
o fe om m| o:n]
.
Pagedof
AT ld WENRONMEPNTARL LOAWBoOECRosATaTOrtRsY
`Thesamplesforthisstudywereprepared asfollows:
1. Theserumsampleswereallowedtothaw andwarmtoroom temperature.
2.
O1n5me mLilslcirlietwe-rcoafpspeerdupmol(yorprwoaptyerleinnetcheenctraisfeuogfe
method tube.
blanks)
wasaddedto
a
3. Matixspikes andmethod blankswer preparedbyspikingthescum (or
`swuartreorg)awtietthotahlelsanearluymtessaomfplinetse,rceaslti:bIrnataidodnisttiaonnd,a'r'dCsP,mFaOtrAiwxablsaankdsd,eadnads a
``mmeetthhooddbpelrafnokrsmaatnaceconcentrati.onof 3.01ng/mLtoevaluateextraction
4. Ttuwbeosmiwlelrielcitaeprpsoefdaacentdovnoitrrtielxemwiexreeda,dadleldowtoitnghtehseesruemruamupdrtohteecieansttroifuge
precipitate.
5. tThheeprteucbiepsitwaetree.centrifigedatiapproximately3000ro for20minutestopellet -
6. Aaftuertreofmoovrvaanilalfayrsoimls.thecentrifige,thesupernatantwastransferredtoan
tQuaanndteifmimcaatsisosnpweacstraocmceotmryp.lTishheefdoblylhoiwginhgppearrfaomremtearnscewleirqeuuisdcehdr:omatography
Loiqauid ChromatogForaph: Hewlett-Packard Series 1100LiquidChromatograph
CAonalluymtinctaelmcpoelrautmu:reK:e3y0stoCne BetasilTMCis2x100mm, Sumparticlesize.
SFltoowpTratei: 3200m0.W0Lmeiimnui;tnes
Injectionvolume: 5uL.
SMolvo entphAba:s2e.i c0ommpMlonaemne mtosn:iumacetateinASTMType Iwater
SSoollvveeanttGBr:aHdiPeLatE:Grade Methanot
--
-
I0me0 a%8
u10s0 2om%
1685500 2o0o%
200 20%
Pages ors
6
--------------------
ECE
ees-------- rwmT ron
mean 50rCose egsive Mass Spectrometer: MDX `Sciex API 4000 Q-Trap Mass Spectrometer Detector
Software: AnalysTMt Version 1.4
GS1:35
GS2: 45
EAnnTtarleayinsci.es TPyoctpveeni:tisMautll:oti1np0lPeaRreaamcetieosn Monitoring (MRM)
FE H reasesE es sR meoT E wse T [ow ee we]|
[onres sso smszoni4mo|
r o--T T srmessaIs |
aot a
F o F nee sTr io me s--i --wmmoaoemwe waamssms soT T |= s m n |||
omar we
wes | 4 |
l Quanity ee ach analys te, hereei bcyopmroevindo itngegdreeatn etr slcasiotnis victyutrhatn iTnIdCiv)iwduaal Qs3dfon
edo
4.1 Calibration
4A 1 Calbatin Cave ia natoesapies, Stok sluons Anextractedcalibrationcurvewas formedbyspikingrabbitserumatprescribed
`wereindividuallypreparedfromneat fluorochemicals and diluted in methanol (except PFBA, which was prepared in Milli-Q water to prevent esterificationfrom
a wi ric cvs meg 1 occurring). Samples were quantified versus an 8-point extracted calibration curve.
]
resorts
_
-_--
WEMROWERNTeAcLtLAoBOCRaAsTaOrReYs
Calibrationstandardswere within + 20% (25%for the LowerLimitofQuantitation (LLOQ) with 2coefficientofdetermination >0.995. Low-level calibration standards,whichwerenotwithin 25%,werenotincludedinthecalibrationcurve.
Calibrationstandardsthatwerenotgreaterthantwicethe methodblankswerenot included in thecalibratcuiroven.
412 ContinuingCalibrationVerification(CCV)
AContinuing Calibration Verification (CCV)wasrunatleastonceevery10
swaimtphliens,2br5a%cfkoetrianlglatnhaelsyatemsp.leswiththecalibrationcurve.The CCVrecovery was
413 SystemSuitability
|
Fivesystem suitabilitystandardswereanalyzedbeforetheinitialcalibrationcurve
andafter the lastCCV. These sampieshad area counts with a%RSD of 5% and a
retention time%RSDof 2%whenevaluatedindependentlyexceptforthefollowing
instances:
Severalindividualtransitionsmonitoredforthe CioAcid failedthe retention time
sqyusatnetmitsautiitoanboiflitthyecrTiItCer,iwah. iHcohwepavsesre,dthseydstaetmasrueiptoabritleidtwyraesqudierrievmeedntfsr.om the
-
All ofthe system suitabilitysctsfor FBSA areacounts failed. The %RSDswere
-
t6h.i2s%an,a7l.y5t%etahnerde8f.o4r%et.hHeodwaetvaewre,snaoccseapmtpelde.shadareacounts above theLLOQfor
414 LowerLimitofQuantit(aLtLiOoQn)
`TheLLOQforeachanalytewas equaltothe loweststandardinthe calibrationcurve
thatmetaccuracyrequirementsof 75% -125%.
415 LimitofDetection(LOD).
`hTahteLwOerDf2o-r3 camcehsaanbaloyvteewthaesmeeqtuhaoldtboltahneka.reacounts(convertedto a concentration) 416 Blanks
Method Blanks
Allof the methodblanks inthis study hadareacounts < 50% of the LLOQ. MatrixBlanks
`Twomatrix blankswerepreparedinrabbitserumandanalyzed withthe samples.
Onematrixblank containedendogenouslevelsof PFOSthatwere abovetheLLOQ. Anaverageendogenous valuefor PFOS wasdetermined bysubstituting a number `equal to %theLLOQ for BLK050203-003.
PageTof 18
--------------
SMENVIROMER NTALLC ANBOOREAT oTOsRY.
Tabl6e. Average Endogenous PFOS Value Determination
I emeswmsos| mesos
<uoa oam
| |
"rTehsepsovneserfagoermeenneddooggmeenneoouusslPeFveOlSsvoaflPuF| eOwSaisusreabdobittcssoerresucmt.the at| xspike
417 SolveatBlanks Acetonitrile blankswere
analyzedthroughout
theanalytical
run.Analyteresponsewas
ssthan %theLLOQ inallcases
418 Surogate
1``3meaxttPrraiFcxtbOiloAannmkwesat,shaaonddddpemedreaftsohroamdsaubnrlcraeon.gkasAtlaetltosacauolznrlcosegenarterurametsciaoomvnpelroeifse3,s.cw0ae1lrinbegrw/aimttiLhointsonteavna2dl0au%ra.dtse,the
4.2
Laboratory Control Spikes (LCS) Six LaboratoryControlSpikes(LCS)
werepreparedin
rabbitserumandanalyzed
2
withthe samples.Results werecorrectedfor cadogenouslevelsofanalytein the
`matrix (whereapproapndarreipreascatteedbe)lo,w.
Table7. Matrix SpikeRecoveries inRabbitSerum
br: aHIE BECERNeaOL a deeSsEos REY
reacass Tocoummon
LLOQ= 3.00nmi. Coomons |
am
75s
| cuenIm
| no | we
|
Comsemons | mss | ma | sox |
rer
1
1
SA au Svansm, lcomcorsozosoe[|s ryso |oa[e es |
locosoapeseac0ss | | mir | wm| ox |
NA ot Applets
Pagetorts q
n
3h of gree
Tabl7e.Mas SpikeRecoveries nRabiSern cota) aMEmmOMEPNRTACLTLusOERnAoTtOeRsY
EE oye 8 -
f[oocommomasnoana|| ssom | <cuuobea T | m wa ||
LoPanAsaCtnHomt |I oOGgmmsaoonrTTrems | cmtieonT | om w |
[orTcosoasaoe-- |T ves | sT ez | vamT |
To
[oowmmson 3m |. <uoa | wa | [owen Tso | cuca | wa |
Loass7es2n3o0m. [[oCGoosooemsmmasooasn|T|esvsams | Tw<i#oo0aT1|1eonmxa]]|
-- F[asomocmI -- ooo[|sse76wa5| m|s<oueosoaet s||t wm]l
can oI was|a am <uoa|--w-- m]
UoQsaizngm[|cOoCmHmGaOsN[|Taess T|rieose | | wewn]|
[comes| 7s| @m1 | ex |
RC
eZ|
LioascazumTaegm T= oommnan|Taw [a aw em |
i
[Fooosmmsoos7s3----1 as wos]
[ ccosasaoe | 73
ma | om |
CRAD
[I omen EsTw 5er | ow]| |ocwmsas| 3e0- | am | oo |
.
LoQmararnome[|eOoCmoasnmoonn|| 775586T | raz a | ioomw ||
A= otAppian[como| vse | 783 | vom]
Pageor18 io a ------ --i------------------
rari
aMEWROWES NTALo LSORAr TORY
"Tabl7e.MatixSpikeRecoveries aRabbi Serum comansd)
EnERI
SE oil RE
rehF|irEa B z aE le E
Ton LT LE RO TLL Howe
[oco | sao onm| soasor| [Cocososmsora | sor | sos |
tex
|
[I Cocomensoss| ao--|--ae | wx |
[Coc | oas orm| soao m s| [Cocosaovoos| sor | ars |ww
|
--r + -- --r--
or
1
[oc|os amo s m |aao m n|
[Cooosameor|a aoa _| sos | ox|
L10a=c0o3e0d3net.[[[oCo coCcsessoOemze amRczonOnss[||T rssmeaa a | | 21m8sa e ||oe=mwv | |||
[oomomzom| aw | ass |wx |
PRGA
[Cocosoamaom| [Cocosazoss|
zee | 2s |ew | ase | aes |ww |
Loawomsnom,| OGGooa | [Cocosomacos|
tse 76
| m2 | ww | | mea | om |
[acusoa[ a00s.|me [mes[ex | rr Tr 1
1
cosmo | am | am |
LLoax 003nom | OCostmvoos| res | me | wx |
Cocowmvos| res | mr | wees |
rr Tr
1
rr
1]
[Cocosomsos|
Lwoawosmnomt[coos |
Cocosmvoos |
300 | 2m | om |
se | me | sew|
rss | mao | woos |
Page100618
IW
ee
uBwmOmEH ITA AS soToRY
Table 7. Matrix Spike Recoveries in Rabbit Serum (continued)
EERE
SECT
he
ES0
! HE
RE SE
L0a=oPsrressro[m.o|c[ o"oscmoaummmC oeosseT|o rm3gsm--c T =|a ao aosrTeus oy] om ]|
F | ccosmsoosr Tr |e mia |s ow |eee]
uoa=ostseom.| acoso | res | raz |ex | _r | acosoTr osTeee Teen | .
ocoszmnon | am: | se |ow |
Uoa=erm nomi|_oommen | rg oe] [CreromcmsewmI meons[ [ma oT | m sT es T |pxT | commis | sa 00 Te]
I - = [ocosmmvooTres TpTie |
[oc | oves s mm aaTo ies|
12
EE ------------------l
Pagetiaris
a
sue soA RATE ORY Tiavtelies. FOS Mats Spt Racoey in Rai Scum, Comactodr Endep
FLElP e ine AOE ERLL
TER
=a
Err os i {co[Cmocmtommnme| sssoosn[|oTamuT[|eowwes
||
aasms
|oT x|on | am |
[ocowmmmoes|sos[31| oss [am |ox|
[[cCiocmoaa|| rmae||rraes || oowsee || mmas| [weee||
4.3 Accuracy
`Theaccuracyforeachanalyte reportedwascalculated by takingtheaverageofthe `matrix spikerecov forethrati anaelys te.Theresults are presenbetleowd.
Table 9. MethodAccuracyforIndiviAndaluyatels:
SE Pec
45
[[pCehewwT|oooe|||| oFmehssaa|| acoox ||
[[eCreasrea || ecmox|||| reorsaas || esomx||
[[opormoog|| [Gioaee |
eeamx ars
|||
|| |
prewoss| | Toros|
womx em
|||
T se ea r[Cmrae ss|TeR mS|S| ToirosS | oe x| nin
4.4 SampleRelatedComments Theextraction andanalysis ofthesamples wasperformed utilizing 2nop-validated `method.Thematrixspikeresults, 'C PFOAsurrogaterecoveries,andthe
extrapolatedcalibrationcurve(betweenthe LLOQandLOD)shouldbeconsidered when evaluatingtheaccuracyandprecisionofthesample results.
Tables 10 and 11 summarize individualsampledata.
1%
Page 20118
Table 10. Parluoinated Acd Results
SM ENRONMER NTALeisome aTaoRY
SES Ra ets a
Ly|
DRE
Ee wTHg ReToad ERA
[[m[RLaoFueseessmeE oT[|aosS s5so%o T[|To"o0g<s0w00[f |awwoa 0ooo|||l <<0oo00n|T|BEo4sssatsB [||ow1L ro82ew1||<I woo000o||["wwoooooo|||a<sciiocooo]|]
[[FDmaaseeue1I7"a0To8ln[[accooo0[<wo0o0|[<C<oT000||E 77069 [[T t2as||Cww=oooo[|sYwooS no|| CccToooo]]
[[moieems1T4ossts"|0<000 wwoooo[|wwoooo||431%8|2w4ri5||a000||asmooo| soioon]]
[[tmessmsTeon acsooT|swooo[[u<s6e0r|5s%s]| ist | woo| woo | too]
Pugs 13001
1%
-- uEROEITA AsCRTAToORY
Table 10. Paruorinatod Acid Rts (continued)
re a. Tr 5 Ti PAHS
WE ER
i
TE
[nat SC RT
a Ee oe ee ae
wh
roES RR
Ban
Cae A
Tapes
[Maeorr=| [meses |
50 ere
| 00| op|<00| 6am[ |26[woo[oo|er|
woo| <00| oo| <oo| 1s|<oo |<oo |<oo|
[assez[aa | [muaesot|<00|
on| oo | woo| op[woo[oo|
rs|ow | 11s[eww |
woo| oo |aoo| <oo |<oo| <oo|
[naesos [kssor
[0s | soo|me | tor|ass | ss|ao0[woo[woo|see
|zes | |zm|
oo <oo
| |
oss |woo ase| <oo
| |
[a7ooo[334 [uaroos [ate
| do | op[woo| amo|tes | <oo | | son[woo | woo|ats |1m | <oo|
00| <o0 | oo| <oo|
[esos [<0 | ao | oo | [ukesss| 00| 00 |woo |
woo|soa |osm | woo |2m |os |
oo| <oo|woo| <oo| osu| oo|
[`mkeost| 5% | [Thesso |<io0|
aco|woo|wo|s@ | te 400|<oo|<o0|sar|ores
|<oo |osm |wow| oo
| |
<oo| <oo |
~
[ess |oer| [sess| <coo|
son| oo| <0o|sas[ores woo|<o0 | oo|2s |ose
| oo| | <oo |
oo| <oo |
<oo| <oo |
Minis | oo oo|<on[00[sm[oss|<00| woo|<o0| [Tksoss|
[oss |
oo| ao0|<oo[woo|ser| 1s |<oo | woo| too | 00 [woo|woo| ems|13s | <oo|woo|
soo| oo|
[aesro [<0 | too | op | [mu esr2| 00| op|oo|
woo|soo | te |<oo| <oo|340 | too | <oo |
aco |woo| woo| soo|
[unesre=|<top| [unsere| oo|
op|<op | woo|<oo |
oo|<too[cto woo |am |ose
| <oo | |<oo|
oo | woo| aoo | soo|
[[aasssseesz||<<o000[|d4o0o0|[oooo[|owooo||z14ss| |oossesss||s<0ooo| |dwoooo| | <<ocoo||
[aosos |asa | son|<oo| woo| zoe | oes | <oo | woo | <o0|
Pe o eeTamTo) [Tween [35 ast| Wa itetan 333[2s |wx
oss [der[12s |
osse |4s [too|
wa |oem | wa |
wa | oem | wa |
[hun
[Boe goswims[ea&ryt1[toswamdo e|Toao10rbt0ooeem3mlp [[roloepls=miscer 1bp0[ |ot0oatsypotw 5nsi[3obe%w emcs[osiwandon:s [[3oss mcel;||o|| [tow
| rae oso
| 3s [24s
[WA | tor |mo| zes| | wa [oss|oem[ow|
wa wa
| ose|wa| |ose| wm |
fn
\
-_--
er
ewR eSesoE wron Table 1. Sample Rafo sAdotnai Pefucrinaatnd HighlyFiorinatedAnsiytss
RE eee ee ah
4
ro
t
i
sib
=5
A (Ee Hii
Pon ARR
maemo T<0|<oo | 00fam |20| op |ome |
[ [w we ee ss tTT7o20o0| s2o00o|tooomme|o arse|r 204|<aooe0T |oooerer]|
[wes [Teo
T<00| <o0| woo[a7 T7200 | <oo[ocr| 4s
| ws |woo|ooew | ves| woo |oon
| |
[--TwAser [200 |oser | aos |= az | 215 | woo | coer|
Heere ees [Tve<or 0|
[wer |"<00| woo|oss |oa | sor| 00|oowe|
[mess [woo 700 ["<00 [tm | 3s | <oo |oer |
.
[meow 100|<00[ows[am | Tuer[06 | <o0[ooew| as
| |
tar| 62 |
<o0 | aos | <oo|ooror|
[wmToo[400|ose [aoe "won |<00[oot
[Tse | ze|<00 | 40 | 22| <o0
| come | |ooeer |
[| TaTwmes ssTTT04000[0wo0o| aeammo ||izrm| |aisme| |<<ooo0||oceeer||
[ [e wes eo n T "T7<00[0 60 ||owmea0 dr||s3a0s| |7 wmsa ||<<i0 o0 6 ||oommeerr||
[ [mTeess sTTT7e0000T|o<oop|[o<moe0r||~s3am"|| 2to1r| |<<ooo0||oooowmo||
|_T"w acoA ["e aoos |cow er|
[wwe "ao0 | doo["ooms | 4m | 17s| woo|oomr|
[oem [wee
Soo sor for aw "T"<00| 00| ao0|"2s
|
aoe ear|"<60
|oor
| |
[e|swsee sT]o<o 00[|w<oo0o||wwoono| | wresr || vwiess || woooo ||oovoemr|]
Page 15ers
16
a
emoAsT can ible 1. Sample autoAdore PceriatadrdHighly Flsintnd
Analytes (continued)
Brdiliat
3
It
[Twaems | 00| <o0| oo[ tm | 6s |<oo|oor |
[Mean [wa [Mew wa
[oom [oases| zr | [oor[omer| sar|
tes| wes |
Wa |ooew | wa|oon |
on | Wa |oir[ooos|sm | me | mw |ors |
tow | wa Toostr [om |omo| art| wa |oow |
[L T r oR sEmT]nors L Rsoeonor em [oTo | 1:|an | wn[oar| Coon[0 | |= | =[%| o |=|
Til 12. mmcRatonfor ialSls
TORSR LT
i x BE rt ; Ee ib pa
=
[ nwewo | coma
i
E
LHC
cn pe
EH
| vx| wx |
[wew sw | e | reweoms peennn || osx | wx |
wes mwmw
| Neva | wen
| ax | wx | | aes | wx |
[ews vse
| een | wows
[aw[we | | ex| ws |
mame wen
| wees [ax| ex | | eos | ow | ax |
mwas | woe | ex| ex |
[rower teem
[them [mes [aes
tame
| tems | | wes [em
|
ex
|
| comes | cama
| aos | wx | | ex | em |
| coma | cweea
| aow | ex | | ww | ux |
CS ET 1
|
----
-_Lo
0 -
anoTnsanpy Tabla 12. lama Ration forict Samples(conte
a wai s et -- e w -- L T Te ] ] wTaT n--T ----i ---- a --
e Rweer T e =e = -- a e e = n|| o weemn T m ee e ] -- EO -------- I T r-- ee e -- =Te e -- nT om n RR *Sample valu cae was < A 7 LLOQ mm tod
Alloriginalrawdataanid theanalyticalreporthavebeenarchivedatthe 3M reserve samples, as wellasthesamplespertainingtothisprojectarearchivedatthe
em ra `Reservesampls,digitalcopiesoforiginaldataandalloriginalpaperdatawill be 70 yes lovin repo ging. retainedinthe archives of 3MEnvironmentalLaboratoryfor a periodofat least
`Underthecondiotftiheostnuds y, endogenous fluorochemicwearles found to be presentinall samples. PFHS, PFOS,andTHPFDS weredetectedineverylot of
Suprmiwehilde FORws proi Bom Tove mm Csoanmvpelresseulys,inFgBtShAe,prTeHsePaFtOmSe,thaonddoltohgeyC.yaCciPdFwOerAesnuortrodgeatteectreedcoivnearineyos fwetrheeall
18 rn eeeeee -------- eeeee
[ I sensu
edd
,Project|
ales
ae -- . o8folr WiliamReagen,LaboratoryManager
Date
`The 3MEnvironmentalLaboratory's QualityAssuranceUnithas auditedthedataand
refoprthoispr rojetct.
a
`QualityAssurance Representative
Dats
4
I
---
AUG 12.2005 SIN
GUALITY ASSOCIATES 28 01
8.Stgmetwess TUTIUT
amos:W0o75no 1
ieee
---
o DE ae -- lor i
`rThee3fMpxEtahvoiisrpcrruomjeetcntt.slLaboratory's QualityAssurance Unitkas andited the data and
NE
xs
TEAR
=
RECEIVED TIME AUG. 12. 4:48PM
F--
PRINT TIME AVG 12. 4:49M 20