Document 0JKKG7oQYzXadwa8NnoXE41Vk

(Me0 Surface Protection Solutions 4417 Lancaster Pike, BMP23-2236 Wilmington, DE 19805 ~C. et :M Marsi - DuPont Malinowski - DuPont C93 10 : 34 A~.aaE ~3,a~ ot a(o May 30, 2008 Attn: Jim Willis (phone 202-564-0104) Document Control Office (7407M) r+~ United States Environmental Protection Agency (EPA) Office of Pollution Prevention and Toxics (OPPT) EPA East, Room 6248 1200 Pennsylvania Avenue, N. W. Washington, D. C . 20460 Dear Jim, As part of DuPont's voluntary sharing of information on our biodegradation research with EPA, enclosed is a Company Sanitized copy of the final study report for a two-year aerobic soil biodegradation research study conducted by Wildlife International (Project No. 112E-108) for DuPont . We would like to meet with you and discuss the study and our interpretation of the results. We will follow up in the near future to schedule time. We plan to publish this work and anticipate submitting a manuscript this summer. Thank you for offering to wait to post this to the Docket until publication. We don't think this will be necessary, however we can discuss it further when we meet . Please contact me if you have any questions regarding the study. Kind regards, IW'-i sa Robert C. Buck Tel. 302 892 8935 ; Email robert .c.buck@usa.dupont.com Company Sanitized I Z110 L( Company Sanitized . Does not contain TSCA CBI . [ ] : TRANSFORMATION POTENTIAL IN AEROBIC AND ANAEROBIC SOILS WILDLIFE INTERNATIONAL, LTD . PROJECT NO.: 112E-108 Organisation for Economic Cooperation and Development OECD Guideline 307 AUTHORS : Edward C. Schaefer Joel Stenzel Raymond L. Van Hoven STUDY INITIATION DATE : March 10, 2005 STUDY COMPLETION DATE: March 17, 2008 Submitted to: E.I. du Pont de Nemours and Company Wilmington, Delaware 19898 USA Wildlife International, Ltd. 8598 Commerce Drive Easton, Maryland 21601 USA 1 (410) 822-8600 Page 1 of 298 Company Sanitized ca ,-, .-- 3 I 20Z( p. 3 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No . : 112E-108 -2GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT SPONSOR : E.I. du Pont de Nemours and Company TITLE: ~ ~ Transformation Potential in Aerobic and Anaerobic Soils WILDLIFE INTERNATIONAL, LTD. PROJECT NO . : 112E-108 STUDY COMPLETION DATE: March 17, 2008 This study was conducted in compliance with Good Laboratory Practice Standards as published by the U.S . Environmental Protection Agency in 40 CFR Part 792 and OECD Principles of Good Laboratory Practice (ENV/MC/CHEM (98) 17), with the following exceptions : Periodic analyses of well water for potential contaminants were perforined using a certified laboratory and standard U.S. Environmental Protection Agency analytical methods. The characterization of the test substance, and the stability of the test substance under the conditions of storage at the test site, were not determined in accordance with Good Laboratory Practice Standards. Original documentation for the preparation of two combined stocks were not maintained . Documentation of the stocks preparation was re-created from the information on the stock label. STUDY DIRECTOR : > Edward C. Schaefer Director of Biodegradation l 7 &42c * j?..eop _ DATE Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -3- QUALITY ASSURANCE STATEMENT This study was examined for compliance with Good Laboratory Practice Standards as published by the U.S . Environmental Protection Agency in 40 CFR Part 792 and OECD Principles of " Good Laboratory Practice (ENV/MC/CHEM (98) 17). The dates of all inspections and audits and the dates that any findings were reported to the Study Director and Laboratory Management were as follows: ACTIVITY : DATE CONDUCTED: DATE REPORTED TO : STUDY DIRECTOR : MANAGEMENT : Protocol March 1, 2005 March 1, 2005 March 11, 2005 Stock Solution Preparation March 15, 2005 March 15, 2005 March 16, 2005 Headspace Sampling March 24, 2005 March 24, 2005 April 13, 2005 Stock Solution Preparation April 19, 2005 April 19, 2005 April 22, 2005 Analytical Data - Day 0 Analyses October 10-26, 2005 October 26, 2005 November 8, 2005 Analytical Data - Week I Analyses Analytical Data - Week 2 Analyses October 10-28, 2005 October 10November 1, 2005 October 28, 2005 November 1, 2005 November 21, 2005 November 21, 2005 Analytical Data - Month I Analyses October 10November 1, 2005 November 1, 2005 November 21, 2005 Analytical Data - Month 2 Analyses October 10November 1, 2005 November 1, 2005 November 21, 2005 Analytical Data - Month 4 Analyses October 10November 2, 2005 November 2, 2005 December 1, 2005 Soil Moisture Determination and Dosing Data October 10December 2, 2005 December 2, 2005 August 30, 2006 Oxygen Measurement October 17, 2005 October 17, 2005 October 19, 2005 Dosing Viability Controls October 17 ; 2005 October 17, 2005 September 26, 2006 Microbial $iomass Sample Processing October 18, 2005 October 18, 2005 November 3, 2006 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 ACTlVITY: Analytical Sample Processing (6-month) Month 6 Analytical Data Analytical Draft Report 9 and 12 Month Analytical Data and Tables Biological Data and Draft Report Data and Draft Report (including 15, 18 and 24 month analytical data) Final Report -4- QUALITY ASSURANCE STATEMENT (continued) DATE CONDUCTED: DATE REPORTED TO : STUDYDIRECTOR : MANAGEMENT: October 20, 2005 November 14-17; .2005 November 17-22, 2005 August 21-25 and 28, 2W6 October 23 November 9, 2006 October 24 November 2, 2007 October 20, 2005 November 17, 2005 November 22, 2005 August 29, 2006 November 9, 2006 November 2, 2007 October 24, 2005 November 28, 2005 December 1, 2005 October 3, 2006 December 18, 2006 November 20, 2007 March 17, 2008 March 17, 2008 March 17, 2008 All inspections were study-based unless otherwise noted. 67~d~- KYtaa Linda R. Mitchell Director of Regulatory and Ecotox Operations /7 /u"t-JI .2Da6 DATE Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -5REPORT APPROVAL SPONSOR: E.I. du Pont de Nemours and Company TITLE: C ) Transformation Potential in Aerobic and Anaerobic Soils WILDLIFE INTERNATIONAL, LTD. PROJECT NO. : 112E-108 STUDY DIRECTOR: Edward C. Schaofer Director of Biodegradation 17 P~-4t-C fA 2-0 &P DATE aK :n t'1, .J ?1 A ~ DATE MANAGEMENT: 1rD_ Y~r~ e!_ Henry O. Knueger, Ph .D. Director of Aquatic Toxicology/Terrestrial Plants and Insects ~/ 17 U DATE Willard B. Nixon, Ph. . Director of Chemistry ~~/r DATE Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 6 TABLE OF CONTENTS Title Page.. ... .. .... .. . .. ... ... . ..... ... ... ... . .. . .. ... ... ... ..... ... . .. .. ... ... .. . ... .. . .. ... . .. . .. ..... ... . ..... ..... ... .. ... .. . . . ...... .. . .. . ... . .. ... . ... ... l Good Laboratory Practice Compliance Statement .. ..... . .. ... ... .. . ...... ..... ... ... ... ..... ... ..... ..... .. . ......., ... ...... . .. .... . .2 Quality Assurance Statement ... .--.- .--. ... ..... ... ... ............. . ..... ... ..--- .... .. ... .. . ... ... ... .. ..... ... ..... .. . ... ... .. ... ... ... ... ... . . .. .3 Report Approval ....... . .. .... .. . .. ... ... . ........ ... .. ...... ... ... .. ... ... .. . ..... ... ... ... ... ... .. ... ... ... .. . .. ... .......... . .. . ... .. ... ... ... . .. .... . . . . 5 Table of Contents .. ... .. . ... ... ... ... ... ... ... ... .. ... ... .. . ... ... .. ..... ... ... ... ...... .. . ..... ...... ........ ...... .. ..... ... ... ...... ... ...... . .. .... .. . .6 Study Information ....... . .. . ... ... ......... ... ..... ... ... .. ...... ... ..... ... ... ... ...... .. . ........ .. . ........ ..... ... .. ... ... ... ... ......... ... ... ... ...16 Introduction ................ . .. ...... ......... . .. ...... ... .. . .. ... ... ... ..... ...... ... .. ... ... . .......... . ............. ... ........... ... ........ . ........ . ...17 Objective ... ........ ... ... ... ... ... ... ... ... ...... ... ........ .. . ........ ..... . .. ...... .. . .. .... ... ..... ... ... ... ........ .. ........ .... ... .. ...... . ........ . ...17 r Experimental Design ..... ... ... ... ... ... ... ... .. ...... .. . .. ... ... ..... ... ...... ... .. ...... . .. ... ...... ... .......... ... ......... .. . .. .. ....... ... ... . ...17 Materials and Methods .. ... ......... ........ ... ... ..... ...... ... ..... .. . ........ ... ... ... . .. .. . ........ . ............... .. . ...... .. . .. ....... ...... . ...18 Test Substance.. ... .... .. . .. ....... ........ ... ...... .. ... ... ............. ...... ... ... ... ... .. . .. ...... . .. ..... ........ ... ...... .. ... ... ... . ...... . .. .18 Measured Analytes . ... ... ...... ... ......... ........... .. . ....... ... ... ...... ..... . ... ..... .. . ...... ... ..... .......... ... .... .. ... .. . ... . .. ...... . .18 Internal Standards .... .. . .. ... ... ......... ... ........ ... .. . .. ........ ... ...... ...... ... ... .. ... ...... ... ................ ...... .. ... .. . ... . ..... .... .21 Test Soils . . ........ ... ... ... . .. ...... ... ... ... ........... ... .. . .......... ......... ..... . ... ... .. ... ...... ... ................ ...... .. . .. ... ... .......... .22 Test Apparatus and Conditions ..... ... ... ...... .. . .. ........ ... ........... . ... ... .. .. . ...... ... ..... .......... ... . ... .. ... ... ...... ...... . .23 Soil Pre-Incubation . ... ... ...... ... ........ ...... ........ . .. ........ ... ... ........ .... ..... .. . ...... ... .. ... .......... ... .... ..... .. . ... ... ... ... . .23 Preparation of the Test Chambers . ...... ...... .. . .. ..... ... ... ... ........ . ... ..... .. . ...... ..... ... .....:.... ... ...... ... .. . ... ... ... ... . .23 Maintenance of Test Chambers ..... ... ... ...... .. . .. .. ... ... ... ... ........ . ... ..... .. . .. .... .. ... ... .. ........ ... ... ...... .. . ... ... ... :.. . .24 Sampling and Measurements ........ ... ... ........ . .. .. ... .. .... ... ..... ... .... ..... .. . .. ......... .. ... ........ ... ... . .. ... .. . ... ... ... ... . .25 Quality Criteria .... ... ... .. . ...... .. . ........ ...... .. ...... ... .. ... ...... ... ........... ...... .. ... ...... ..... ... ........... ... ........ . ... ... ... ... ..25 Viability Assessments . ......... ......... ........ ... ... ... .. .. . .. ......... ...... ... ... ..... ... ...... ..... ... ..... ............ ..... .... ... ... .....26 Results .. ... ........ ... ... ... ... ... ...... ... ... ... ... ........ .. ... ...... ... .. ... ... ... ..... ... ... . ..... ...... .. . ..... ..... . .. ... ..... ............ . ........ . .....26 Test Conditions .. ... ...... ... ... ... ...... ........ ... ........ ... .. ... ......... ........ ...... ...... .. . ........................ . .. ...... ... ....... .....26 Characterization of Soils .. . ... ... ... ........ ... .. ... ... ... .. ... ................. ... ......... ... .......... ... ............ ..... ... ... ...... ......27 Sample Processing and Analyses ....... .. . .. ...... ... .. . .---- ... ... ........ . ..... ... ... ... ........ .----.- .--....... ........ ......... ......27 Headspace Analyses ... ......... ..... ... ..... ...... ..... ... ...... ..... ... ... .--- .. ...... ... ....:............. ...... . .. ......... ... .........27 Soil Analyses ..... ........ . ........ . .......... ... ........... .. . .. ... ... ... ... ... ... ......... .. . .. ..... ... ..... ... ...... ... ........ . ... ...... ...27 Stock and Calibration Standard Preparation .. ............ ... ... ... ... .. ...... . .......... ... .. ... ...... ... ...... ... ... ... ......29 Calibration Curves and Limits of Quantitation .. ... ..... ... ... ...... ........ ... ..... ... ..... ......... ... ...... ... ...... ......31 References . .. . .. .... .. ... ...... ... ...... .. . .....-- .-..--- ......---.. ........ ..---- ..... ... ........... . ........ . .. .. . .......... ... ... .... .--.-- .. .-- .---.. ....34 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No .: 112E-108 -7TABLE OF CONTENTS - Continued - TABLES Table 1 . Table 2. Frequency of Measurements .. .- .--.- .--.. ... ... ... ... ... ... . .. ... .. . .. .... ... .. .... .. . .--- . ..... . ........ ....... .. . .. ...... .... . .35 Test Chamber Headspace Oxygen Content ... . .. .... ... ..... ... ... ... ... . .. ... ... ..... ......... .. . ...... . .. ...... ... .... .36 Table 3 . Selected Soil Properties . ... ... .. .... ... ........ ......... . ...... ... ........ . .. ... ... ... ...... ..... ......... ... ... ... . .. ... ... ... . ... .37 Table 4. Soil Viability by Glucose Mineralization :Alfisol. ........... . ........ . ...... ..... .. . ... ... .. ... .... ... ........ .... ... .38 Table 5. Soil Viability by Glucose Mineralization :lnceptisol ..... ... ...... ... ... ... ... ..... ... . .. ... ... ... ......... ... ... ....38 Table 6. Soil Viability by Glucose Mineralization:Ultisol .......... . ........ ... ....... ....... ... . ........ ... ...... ... ... ... ....39 Table 7. Soil Viability by Glucose Mineralization:Mollisol .. ..... ... ...... ... ....... .. ........ . ..... ... ......... ... ...... ....39 Table 8. Soil Microbial Biomass . . ... .. .... ... ........ ... ...... . .. .... ... ........ ... ...... ... ...... . ....... ... . ........ ... ...... ... ...... . ...40 Table 9. Typical GC/MS Operational Parameters for Analysis of 8-2 FTOH in Headspace Gas . ...... ....42 Table 10. Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in Soil Extracts .... ... ... ... ... .. . .. . ... ... ........ ...... ... .. . ... ... ... ....:.... ...43 Table 11 . Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTOH in Soil Extracts .. .... ..44 Table 12. Typical LC/MS/MS Operational Parameters for Analysis of7-3 Acid and 7-2 sFTOH in Soil Extracts .. ......... . .... ...... ..... .. . ......... .. ...... ... .... .. . ...... ...... ... ... ... ... .. . ... ... ........ ... ... ...... ... ... . ..... ... ... ......45 FIGURES Figure 1 . Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers.. ... ... ..46 Figure 2. Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts. . . ..... ... ......... ...... ... ..... . ... ... ... ......... ... ..47 Figure 3. Representative ion chromatogram for 8-2 FTOH in a low-level (25 .0 ng a.i./mL) calibration standard by GC/MS ------- ...... ...... . ......... ... ...... ... ... ... ... .............. ... . ..... .---...... ..............48 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No .: 112E-108 -8TABLE OF CONTENTS - Continued - Figure 4. Representative ion chromatogram for 8-2 FTOH in a high-level (500 ng a.i./mL) calibration standard by GC/MS .... . ........ .. . .. .... ... .. ... .. .... .. ... .. ... ... ... .. . .. .... .. ... ...... .. . .. .. .. . ...... .. . .. ... .49 Figure 5. Representative ion chromatogram for 8-2 FTOH in a low-level (10 .0 ng a.i./mL) calibration standard by LC/MS/MS. ........ . .. ...... ... ... .. ...... .. . .. ... ... ... .. . ...... .. ..... ...... . .... .. . ...... .. . .. ... .50 Figure 6. Representative ion chromatogram for 8-2 FTOH in a high-level (1000 ng a.i./mL) calibration standard by LC/MS/MS . ..... ... ... .. . ... ... .. .. . ... ..... ... .. . ........ ... ... ... .. ... ... ... ....... . ........ ..... . .51 Figure 7. Representative ion chromatogram for 7-2 sFTOH in a low-level (5 .00 ng a.i./mL) calibration standard by LC/MS/MS ... ... .. .... .. . .. ... ..... . ............. ........... . .. ...... ...... .......... ... ... ........ . .52 Figure 8 . Representative ion chromatogram for 7-2 sFTOH in a high-level (250 ng a.i./mL) calibration standard by LC/MS/MS ... ..... ...... ... ........... .............. ..... ... ...... ..... ...... ........ ... ... ....... . ..53 Figure 9 . Representative ion chromatogram for 8-2 FTA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standards by LC/MS/MS . ... .. ... .. .... .. ... ........ ... ........... ... ........ ... ... ..... ... .. . ..... ...54 Figure 10. Representative ion chromatogram for 8-2 FTA in a high-level. (5.00 ng a.i./mL) combined PFCA calibration standards by LC/MS/MS ... . .. ... .. ... . .. ... .. ... ........ ... ... ... ... ..... ... ... .. ... ........ ..... ... .55 Figure 11 . Representative ion chromatogram for 7-3 Acid in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.. ... ... .. ... ... ... ..... .. ... ...... .. . ...... .. . .. ... ... ... .... ... .............. .56 Figure 12. Representative ion chromatogram for 7-3 Acid in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS.. ... ... .. .. . .. ...... ... .. ... ........ ....... .. . .. ..... ........ ... .............. .57 Figure 13 . Representative ion chromatogram for 8-2 FTUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS .. .. ... .. ......... .. ......... ... .. . .. .... .. .. ... ...... ........ .58 Figure 14. Representative ion chromatogram for 8-2 FTUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS .... ..... .. . ........ ... ...... ... .. ........... ... ... .......... . .59 Figure 15 . Representative ion chromatogram for PFOA in a low-level (0 .100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS .... ....... . ........ ... ...... ..... ... ... .......... . .......... ..60 Figure 16. Representative ion chromatogram for PFOA in a high-level (5 .00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS ........... ... ......... ... ..... ..... . .. ... .. ... ... ........... ..61 Figure 17. Representative ion chromatogram for PFNA in a low-level (0 .100 ng a.i ./mL) combined PFCA calibration standard by LC/MS/MS . ............. ... ........ . ..... ..... . .. ... .. ...... ..... ... .....62 p. 10 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. Project No.: 112E-108 -9TABLE OF CONTENTS - Continued - Figure 18. Representative ion chromatogram for PFNA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS .. ...... ... ...... ... ... ... ... ... . .. .... .. . ... . .. ... ......... . ..63 Figure 19. Representative ion chromatogram for PFDA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS ..... ... ... ... ... ......... ....... ...... ... .... ... .. ... ... .... ..64 Figure 20. Representative ion chromatogram for PFDA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS ..... ... ...... . .. .... ..... ....... ... ... ... .... ... ........ .... ..65 Figure 21. Representative ion chromatogram for PFUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by GC/MS . . .. ... ... ... ...... ... ...... . ...... ......... . .. .... ... ..... ... .... ..66 Figure 22. Representative ion chromatogram for PFUA in a high-level (5 .00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . ... ... ... ... ... ...... ......... . .. .... ..... .... ... ... ... . .. .... ... ............ ..67 Figure 23. Representative calibration curve for 8-2 FTOH by GC/MS .... ... .... ............... ... ... .... ... ..... ....... ..68 Figure 24. Representative calibration curve for 8-2 FTOH by LC/MS/MS . . ... ... ...... . ..... ... . ... ... ... ........ . ... ..69 Figure 25. Representative calibration curve for 7-2 sFT0H by LC/MS/MS .... . ......... ...... .. . ... . ........ ... ... ... ..70 Figure 26. Representative calibration curve for 8-2 FTA by LC/MS/MS .. ... ... . ........ . ...... ... ... ......... ... ... . .. ..71 Figure 27. Representative calibration curve for 7-3 Acid by LC/MS/MS ... .... ... .. .... . ........ . ... ... ... ...... ... ... ..72 Figure 28. Representative calibration curve for 8-2 FTUA by LC/MS/MS .. .... .. ............. .. . ... ......... ... ... . .. ..73 Figure 29. Representative calibration curve for PFOA by LC/MS/MS :. .. . ...... ... .. .... . ........ . ......... ...... ... ... ..74 Figure 30. Representative calibration curve for PFNA by LC/MS/MS..... . ...... ... ...... . ........ . ... ...... ...... ... ... ..75 Figure 31. Representative calibration curve for PFDA by LC/MS/MS .... ... .... ..... .... . ........ ....... ... ...... ... ... ..76 Figure 32. Representative calibration curve for PFUA by LC/MS/MS..... ... ......... .... ... ...... .... ... ......... ... .....77 Figure 33. Representative ion chromatogram for 8-2 FTOH in the sampled headspace (C18 cartridge extract) from a Day 0 untreated control soil by GC/MS ...... ... ... ...... ... ....... ...... ... . ... ... ......... .....78 Figure 34. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS . ......... . ...... .. . ...... ... ...... ... ....... ... ................ ....... ... ......... .....79 p. 11 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No .: 112E-108 -10TABLE OF CONTENTS - Continued - Figure 35. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS ..... ..... . .. .. ... .. ... ... ... ..... ... ... .. . .. ... ... . .. .. . ..... ...... ..... ... ... ... ..... .80 Figure 36. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 , untreated control soil by LC/MS/MS ....... ... .. . .. .. ... .. ....... .. ... ........... .. .... .. . .. ... ... ... ... .. ...... ... ... .. ... . 81 Figure 37. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 untreated control soil by LC/MS/MS .. ........... ... .. .. ... ... ... ... ..... ...... .. ... ... ... . .. ... ... ... ... .. ...... ... ..... ....82 Figure 38 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . ... ..... ... ....... ... ... ... ..... ... ........ ... ... ... . .. ... ...... ..... ........ ... ....... 83 Figure 39. Representative ion chromatogram for PFOA in the soil extract from a Day 0 untreated control soil by LC/MS/MS .. ......... ...... .. . .. ... .......... ...... ..... ..... ...... ... ..... . ........ . .. ...... .. ... ...... ..... .. ... 84 Figure 40. Representative ion chromatogram for PFNA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . . ... ... .. ... ...... ..... ... ..... ........ . .. ... ..... ... ... ... ..... ... ...... . .. ... ....... . ........... .. . ..85 Figure 41 . Representative ion chromatogram for PFDA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . . ... ..... ... ..:.. ..... ... ..... ........ ... ........ ... ... ........ ... ... ... ... ..... ..... ... . .. ........ ... 86 Figure 42. Representative ion chromatogram for PFUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . . .. ...... ... ... ... ..... ... .. ........ ... .. . .. ... ... ... ... .. ...... ... ...... .. . .. ... ..... ... . .......... ... 87 Figure 43. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Clg cartridge extract) from a Month 24 untreated control soil by GC/MS ... ... ........ . ........ ... .............. .......... ...88 Figure 44. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS .......... . .. ..... .. .... ... .. ... ..... .... .. ......... ........ . .. ........... ... ........ ...89 Figure 45 . Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS .......... . .. ..... ...:.. .. . .. ... ..... ... ........... . ........ ... ... ........ ..............90 Figure 46 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 untreated control soil by LC/MS/MS .......... ... ....... ... ... ... ... .. ...... ........ ... ......... ...... .. .. ... ... ... .........91 Figure 47. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 untreated control soil by LC/MS/MS ............... ..... ... ... ... ..... ...... .. ... ... ... .---.. ... ..... . .. .. ... ... ... ... ......92 Figure 48 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS .. ............. .. ... ... ... ... .. ... ...... ..... ...... ...... ... ..... ... ..... ...... ... ......93 p. 12 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -11TABLE OF CONTENTS - Continued - Figure 49. Representative ion chromatogram for PFOA in the soil extract from a Month 24 untreated control soil by LC/MS/MS . . ... ...... ..... . .. . .. .... ... ... . .. . ... ... ... ... ... ... ... ... ... ..---. .. . .. ....... ... . .. .94 Figure 50 . Representative ion chromatogram for PFNA in the soil extract from a Month 24 untreated control soil by LC/MS/MS ........ ... ...... . ..---... . ...... ... ... . ........ . .. ....... ..... ... ... ............ ... . .. .95 Figure 51 . Representative ion chromatogram for PFDA in the soil extract from a Month 24 untreated control soil by LC/MS/MS ...... ..... ...... . ......--- ... ... ....... ...... ... ... ... ... ... ..... . .. ... ... ... ...... ....96 Figure 52 . Representative ion chromatogram for PFUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS ...... ... ...... .. . ...... ...... . ...... ... ....... ........---...... ... ... ..... ... .... ......97 Figure 53 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (CI8 cartridge extract) from a Day 0 fortified (250 gg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by GC/MS ....---. . .. ....... ..... ... ... .....-.-- .---------- . ... . ...... ... .... ... ........ . ... ..... ... ... ... ... .. .... ......98 Figure 54. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS . ..... .... ... ..99 Figure 55. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 fortified (250 gg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS . ......... ... 100 Figure 56. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 fortified (250 ug 8-2 FTOH/kg and 10.0 jig PFCAs/kg) control soil by LC/MS/MS . ...... ...... 101 Figure 57. Representative ion chromatogram for PFOA in the soil extract from a Day 0 fortified (250 gg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS . ...... ...... 102 Figure 58. Representative ion chromatogram for PFNA in the soil extract from a Day 0 fortified (250 jig 8-2 FTOH/kg and 10.0 p g PFCAs/kg) control soil by LC/MS/MS . ...... .... ..103 Figure 59. Representative ion chromatogram for PFDA in the soil extract from a Day 0 fortified (250 p.g 8-2 FTOH/kg and 10.0 jig PFCAs%lcg) control soil by LC/MS/MS . ... ... .... .. 104 Figure 60. Representative ion chromatogram for PFUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS .... ... .... .. 105 Figure 61 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C8 cartridge extract) from a Month 12 fortified (250 gg 8-2 FTOH/kg and 10 .0 gg PFCAs/kg) control soil by GC/MS ...... . ........ . ................... ... ....... ......... .... ... ...... ...... ..... . ... ... .. . .. .... .. . ..... ... ....106 p. 13 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. Project No.: 112E-108 -12TABLE OF CONTENTS - Continued - Figure 62. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 fortified (250 ~ig 8-2 FTOH/kg and 10.0 jig PFCAs/kg) control soil by LC/MS/MS ... ... .. .. ... 107 Figure 63. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 fortified (250 ~Lg 8-2 FTOH/kg and 10.0 ~tg PFCAs/kg) control soil by LC/MS/MS ... ..... ..... 108 Figure 64. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 fortified (250 gg 8-2 FTOH/kg and 10.0 jig PFCAs/kg) control soil by LC/MS/MS ........ .. ... 109 Figure 65 . Representative ion chromatogram for PFOA in the soil extract from a Month 12 fortified (250 gg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS .. ........... 110 Figure 66. Representative ion chromatogram for PFNA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS .. ... ........ Ill Figure 67 . Representative ion chromatogram for PFDA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.. ... ..... ... 112 Figure 68 . Representative ion chromatogram for PFUA in the soil extract from a Month 12 fortified (250 ~ig 8-2 FTOH/kg and 10.0 ~ig PFCAs/kg) control soil by LC/MS/MS . . .......... . 113 Figure 69 Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C1g cartridge extract) from a Day 0 sterile soil treated with 200 mg/kg H=26634 by GUMS ........... .......... . 114 Figure 70. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 sterile soil treated with 200 mg/kg H-26634 by LC/MS/MS ...... ... .. ... .. ........ ............ .. ... ...... ... ........ ...115 Figure 71. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg (_ )by LC/MS/MS ...... .. . .. .. . .. ........ ..... . .............. ... ... ........ ...116 Figure 72. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kC ]'by LC/MS/MS ...... .. ... ..... ............. . ........ ... ... ... .. . ........ ...117 Figure 73 . Representative ion chromatogram for PFOA in the soil extract from a Day 0 sterile soil treated with 200 mg/kgC_ )by LC/MS/MS ........ ... .. ... ... .. ........ ......... ... ...... .. . ...........118 Figure 74. Representative ion chromatogram for PFNA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg[ ] by LC/MS/MS .. ...... ..... ... ..... ........ ......... ... ........ . ...........119 Figure 75 . Representative ion chromatogram for PFDA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg(' 3. by LC/MS/MS ........ ..... ........ ........ ......... .. . ........ . ...........120 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No .: 112E-108 -13TABLE OF CONTENTS - Continued - Figure 76. Representative ion chromatogram for PFUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kgL ] by LC/MS/MS ... . ...... .. . .. .... ... .... ....... ...... ... ... . .. ... .. .... ... .121 Figure 77. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cl8 cartridge extract) from a Month 12 sterile soil treated with 200 mg/kt ay GC/MS.... ......... ... 122 Figure 78. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 sterile soil treated with 200 mg/kg C )by LC/MS/MS ...... ... ... ... ... ... . ... . .:.... . .. ...... ......... .. .... ... ..123 Figure 79. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC -) by LC/MS/MS ... . ... ... .. . .. .... .............. ... ... ... ... . ..... .. .... ... .124 Figure 80. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC I by LC/MS/MS ..... ... ........ . ...... . ... . .. .... . .. ...... ...... ..... .... ...125 Figure 81 . Representative ion chromatogram for PFOA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg[ Dby LC/MS/MS ..... . ......... ... ... .... . ...... ... ...... ... ... ..... ... .... ..126 Figure 82. Representative ion chromatogram for PFNA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg[ 3 by LC/MS/MS ... ...... ... ....... .... .. .... ....... ... ...... ... ..... ... .....127 Figure 83 . Representative ion chromatogram for PFDA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgL )by LC/MS/MS . ... ... . .. ...... .... ... .... ... .... ......... .. . ... .. ... ... ....128 Figure 84. Representative ion chromatogram for PFUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg C 3by LC/MS/MS ..... ...... ... ... ... . ... .... ... ........---. .... .. ... ... ... ...129 Figure 85. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C18 cartridge extract) from a Day O live soil treated with 200 mg/kg ~ by GC/MS ..... .... .. . .. .. ...... . ..130 Figure 86. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 live soil treated with 200 mg/kgC )by LC/MS/MS .......... ...... ... ... . ...... .... ... ... ...... ........... .....131 Figure 87. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day O live soil treated with 200 mg/kg[ ~by LC/MS/MS . ... ... ...... ... .... ....... ... .... ........ . .............. ... .132 Figure 88 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Day O live soil treated with 200 mg/kgC ]by LC/MS/MS .... ....... .. . ... ... . ... ....... . ... ..... . .. .... .. .. ... .... ...133 Figure 89. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day O live soil treated with 200 mg/kg C )by LC/MS/MS .. ......... ... .......... . ...... . ... ... ... ...... .. ... ...... . ..134 p. 15 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 =14TABLE OF CONTENTS - Continued - Figure 90. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day O live soil treated with 200 mg/kg t 'Jby LC/MS/MS .... .. ... .. ...... . ........ ... .. ...... .. ... ... ... ..... ..... ...135 Figure 91 . Representative ion chromatogram for PFOA in the soil 'extract from a Day O live soil treated with 200 mg/kg C )by LC/MS/MS .. ...... ... .. . .. ...:.. . .. ...... ..... ... .. .... ... .. .......... .136 Figure 92. Representative ion chromatogram for PFNA in the soil extract from a Day O live soil treated with 200 mg/kgL )by LC/MS/MS .... ........... ... ...... ... ... ........ .. ... ... . .. ... .. . .. .. ..137 Figure 93. Representative ion chromatogram for PFDA in the soil extract from a Day O live soil treated with 200 mg/kgfL -3 by LC/MS/MS ... ... ..... . ...... .. . ..... ... ... .. ... ........ . ..... ..........138 Figure 94. Representative ion chromatogram for PFUA in the soil extract from a Day 0 live soil treated with 200 mg/kgL' -)by LC/MS/MS .. ........... ... ...... . ..... ... ..... ..:...... ... ..... ........139 Figure 95. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,g cartridge extract) from a Month 24 live soil treated with 200 mg/kg( ~~y GC/MS .. ........ ... ..... .140 Figure 96. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg C I by LC/MS/MS ... ........: ........ . ........ ... .. . .. ........ . ....---........141 Figure 97. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg j ]by LC/MS/MS .. ...... ...... ... ... ... ... ...... .. ... .. ... ... ... ... .. ..... ...142 Figure 98. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 live soil treated with 200 mg/kg[- " J)y LC/MS/1\4S . .... .. ... ......... ...... ... ........ ..... . .. ...... ... .. ... ...143 Figure 99. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 live soil treated with 200 mg/kg :~ ]by LC/MS/MS . .. . ..... ... ... ... ... ... ...... ... ..... ..... ... ...... ..... ... .144 Figure 100. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 live soil treated with 200 mg/kgC ) by LC/MS/MS ... .. ...... ... ... ... ... ...... .. ... ........... . .. ..... .....145 Figure 101. Representative ion chromatogram for PFOA in the soil extract from a Month 24 live soil treated with 200 mg/kgt )by LC/MS/MS .. ..... ...... ... ...... ... ... ................. ... .. ... .. .. .146 Figure 102 . Representative ion chromatogram for PFNA in the soil extract from a Month 24 live soil treated with 200 mg/kgE )by LC/MS/MS . ........ .:.... ... . ........ ... ............. . ... ....--- .-- .147 Figure 103 . Representative ion chromatogram for PFDA in the soil extract from a Month 24 live soil treated with 200 mg/kg[ ~ny LC/MS/MS ... ........ ... ...... . .. ... ... ..... ... ........... ...---.-- .148 p. 16 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -15- TABLE OF CONTENTS - Continued - Figure 104. Representative ion chromatogram for PFUA in the soil extract from a Month 24 live soil treated with 200 mg/kg~ ,by LC/MS/MS .. . ... .. ... ... . ... ... .. . ... ... .... ... ...... ..... .. . ... ... .149 APPENDICES Appendix 1. Measured Concentration of Selected Analytes (4g/kg Soil Dry Weight) . .. . .. .... .. .. ... ... ...150 Appendix 11. Measured Concentration of Selected Analytes (~tmol/kg Soil Dry Weight) ... ... ..... .. . .....223 Appendix Ill . Example Calculations . . .. ... ... . ......... ... . .. .. . ........ ..... . ... . .. ... ... ... . ... .. ... .... ... .... ... ... ... ... .. ...... .. ..296 Appendix IV. Duration of Incubation ...... ... .... .. . ... ... .. . ...... .. ... .. .... ... ...... .. . .... .. ... ....... ... ... .... ... ............. ... .298 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -16- STUDY INFORMATION Study Initiation Date : Experimental Start Date: Experimental Termination Date: Study Completion Date: 10 March 2005 11 March 2005 07 September 2007 17 March 2008 Project No. : 112E-108 Study Director: Sponsor : Sponsor Representatives: Study Personnel : Edward C. Schaefer E.I. du Pont de Nemours and Company William Berti and Bogdan Szostek Edward C. Schaefer, B.S., Director ofBiodegradation Raymond L. Van Hoven, Ph.D., Scientist, Chemistry Joel I. Stenzel, B.S., Senior Chemist R. Scott Flaggs, B.S., Biologist, Biodegradation Abul Siddiqui, B .S., Scientist, Biodegradation Frank Lezotte, B.S., Senior Chemist, Chemistry David L. Weiss, M.S., Chemist, Chemistry William Schutt, B.S ., Chemist, Chemistry John R. Murrell, B.S ., Senior Chemist Willard B. Nixon, Ph.D., Director of Chemistry Henry O. Krueger, Ph.D., Director ofAquatic Toxicology/Terrestrial Plants and Insects Molly E. Matthews, Biologist, Biodegradation Jon A. MacGregor, B.S., Scientist Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -17- INTRODUCTION This report describes a laboratory research study conducted to assess aerobic and anaerobic transformation of C In soil systems . This study was conducted by Wildlife International, Ltd . for E.I. du Pont de Nemours and Company at the Wildlife International, Ltd. research facility in Easton, Maryland. The raw data generated by Wildlife International, Ltd . and the original final report final report will be archived by the study sponsor. A certified copy will be retained under Project Number 112E-108 in the archives located at on the Wildlife international, Ltd. site. OBJECTIVE The objective of the research study was to assess the potential for transformation of the test substance in aerobic and anaerobic soils. EXPERIIVIENTAL DESIGN The test was conducted with four test soils under aerobic conditions. The study was initially designed to set-up to also include four soils under anaerobic conditions, however a check of the system indicated that the test chamber closures did not exclude air. The systems initially started under anaerobic conditions up to and including month 6 are included in this report because they were extracted'and analyzed as planned . Four groups were established for each soil type and condition (aerobic or anaerobic) : (1) untreated (control) live soil; (2) 200 mg test substance/kg dw treated live soil; (3) 200 mg test substance/kg dw treated sterile soil; (4) 250 ~ig 8-2 telomer B alcohol/kg dw treated sterile soil; 10 ~Lg 8-2 telomer B acid /kg dw; 10 ~ig 8-2 unsaturated telomer B acid/kg dw; and 10 ~Lg perfluorooctanoic acid/kg dw, heptadecafluorononaoic acid, nonadecafluorodecanoic acid, and perfluoroundecanoic acid at 10 p.g/kg dw all added to sterile soil. The test systems reported here were incubated in sealed containers at approximately 20C for at approximately 24 months . Two incubation chambers from each of the groups were removed at appropriate time intervals and the soil samples were extracted and analyzed for potential metabolites . In addition to the day zero samples, eight samplings were performed with all of the groups . Three additional sampling intervals were performed with only the untreated control (1) and 200 mg test substance/kg dw treated live soil (2) groups . Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. Project No.: 112E-108 -18- Additional untreated and test substance treated soil chambers were prepared for use as matrix fortification samples, biomass determinations, viability controls and to monitor aerobic conditions as necessary . " The test groups were prepared using 25 grams (dry weight equivalent) of soil. While this amount of soil is less than the 50 to 200 grams specified in the OECD 307 guideline, it was chosen to allow for 1) the extraction of the entire test chamber contents and internal surfaces; 2) sufficient headspace to minimize the need for active aeration which could result in a loss of potential transformation products. METHODS AND MATERIALS The test system and study conditions are based on the OECD Guideline for Testing of Chemicals, Guideline 307, Aerobic and Anaerobic Transformation in Soil (1). As this guideline is designed for low molecular weight substances, but not for polymers, adaptations of the method may be necessary for feasibility reasons . Nevertheless, the study was carried out in such a way that the recommendations given in the guideline were followed as closely as possible. Test Substance The following substance was used in this study : ID:( Date received : 2 November 2004 Wildlife International Ltd. ID: 6918 Measured Analytes Name : 8-2 Telomer B Acid, 8-2 Fluorotelomer Acid Synonym used in Report : 8-2 FTA CAS Name: Decanoic acid, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoro-(8CI,9CI) CAS Number : 27854-31-5 Molecular Weight: 478.1 g/mole ID : H-26210 _ Date received : 1 September 2004 , Expiration/reanalysis date : 12 October 2007 Purity : 97%; 3% 2H-Hexadecafluoro-2-Decanoic Acid Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -19- Description: Solid Wildlife International Ltd . ID: 6826 Name: 8-2 Telomer B Alcohol, 8-2 Fluorotelomer Alcohol Synonym used in Report : 8-2 FTOH CAS Name: 1-Decanol, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoro- (7CI, 80, 90) CAS Number: 678-39-7 Molecular Weight: 464.11 g/mole ID: H-24691 Date received : 1 September 2004 Expiration/reanalysis date : 12 October 2007 Purity : 99 .2% Description : Solid Wildlife International Ltd. ID: 6828 Name: 8-2 Unsaturated Telomer B Acid, 8-2 Fluorotelomer Unsaturated Acid Synonym used in Report: 8-2 FTUA CAS Name: 2-Decenoic acid, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-hexadecafluoroCAS Number : 70887-84-2 Molecular Weight: 458.1 g/mole ID: H-26211 Date received : 1 September 2004 Expiration/reanalysis date : 12 October 2007 Purity : 98%; 0.5% 8-2 FTOH ; 1 .5% unknown Description: Solid Wildlife International Ltd. ID : 6829 (9CI) Name: N-Perfluorooctanoic Acid Synonym used in Report : PFOA CAS Name : Octanoic acid, pentadecafluoro- (8CI, 90) CAS Number : 335-67-1 Molecular Weight : 414.07 g/mole ID: H-24749 Date received: 10 September 2004 Expiration/reanalysis date: 12 October 2007 Purity : 99.2% Description: Solid Wildlife International Ltd. ID: 6849 Name: Heptadecafluorononaoic Acid, Perfluorononanoic Acid Synonym used in Report: PFNA CAS Name: Nonanoic acid, heptadecafluoro-(8CI, 90) CAS Number : 375-95-1 Molecular Weight : 464.08 g/mole ID: H7568 Date received: 30 November 2004 Expiration/reanalysis date: 12 October 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife InteYnational, Ltd. Project No.: 112E-108 -20- Purity: 99+% Description : Solid Wildlife International Ltd. ID: 6944 Name: Nonadecafluorodecanoic Acid, Perfluorodecanoic Acid Synonym used in Report: PFDA CAS Name: Decanoic acid, nonadecafluoro (6CI, 70, 80, 90) CAS Number : 335-76-2 Molecular Weight: 514.08 g/mole ID : RI 1K Date received : 30 November 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99+% Description: Solid Wildlife International Ltd. ID: 6943 Name: Perfluoroundecanoic Acid, Perfluoroundecanoic Acid Synonym used in Report: PFUA CAS Name: Undecanoic acid, heneicosafluoro-,ammonium salt (9CI) CAS Number: 4234-23-5 Molecular Weight: 564.09 g/mole m: Q30G Date received : 30 November 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99+% Description : Solid Wildlife International Ltd . ID: 6945 Name: 2H, 2H, 3H, 3H Pentadecafluoro-Decanoic acid Synonym used in Report : 7-3 Acid CAS Name: Decanoic acid, 4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-pentadecafluoro- CAS Number: 812-70-4 . Molecular Weight: 442 .12 g/mole ID: 26833 Date received :,20 April 2006 Expiration/reaalysis date: Not provided Purity : Not provided Description : Solid Wildlife International Ltd . ED: 7592 Name : 1-Perfluoro heptyl ethanol, 7-2 Secondary Fluorotelomer Alcohol Synonym used in Report : 7-2 sFTOH CAS Name: Not provided CAS Number: Not provided Molecular Weight: 414 .11 g/mole ID: 27497 Lot: 72SFTOHGA05 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -21 - Date received : 20 April 2006 Expiration/reanalysis date: Not provided Purity : Not provided Description : Liquid Wildlife International Ltd . ID: 7593 Internal Standards Name: N-Perfluorooctanoic Acid [1,2-di-13C] Synonym used in Report : 2-13C PFOA CAS Name: NA CAS Number: NA ID: H-25617 Dates received: 1 September 2004 and 20 April 2006 Expiration/reanalysis date: 12 October 2007 and 31 January Purity : 96.4% (not given for W1L 7591) Description : Solid Wildlife International Ltd. ID: 6827, 7591 Standard Reanalysis Date: August 2007 Purity: Confirmed Expiration Date: August 2010 2007 Name: 1D, 1D, 2D, 2D, 3-13C-Heptadecafluorodecanol Synonym used in Report : HDFD CAS Name: NA CAS Number : NA ID: H-25433 Dates received : 10 September 2004 and 20 April 2006 Expiration/reanalysis date : 12 October 2007 Purity : 100% (not given for WIL 7590) Description: Solid Wildlife International Ltd. IDs: 6850, 7590 Project No. : 112E-108 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -22- Test Soils Four freshly collected United States (U.S.) soils were used to evaluate the test substance under aerobic and anaerobic conditions. The soils were collected from each of the following soil orders : Soil Order Percent of US Land* Alfisol 13 Mollisol 25 Inceptisol 16 Ultisol 13 *Percent of total land in the US, from Foth, H.D . 1990 . Fundamentals of Soil Science 8 ed. John Wiley & Sons, New York) The soils were processed between 6 and 22 days after collection. Processing included removing vegetation, larger soil fauna, stones and passing the soil through a 2 mm sieve . The soil used in the sterile groups was sterilized by cobalt irradiation. The soils were stored in the dark at 4 2 C. Characterization of the soils was conducted by Agvise Laboratories (Northwood, North Dakota, USA). The following is a list of the minimum physicochemical properties ofthe soil. " Texture (i.e., percentage of sand, silt and clay) (NUT.02.06) " pH (NUT.02 .05) " Organic carbon (NUT.20.20) " Bulk density (NUT.02.10) " Field moisture capacity (% moisture at 1/3 bar) " Cation exchange capacity (NUT.02.03) The percent moisture and water holding capacity (ISO 14240-2 ; Annex A) of each of the soil types was determined . In addition, the microbial biomass (ISO 14240-2 ; fumigation-extraction) of a live sample of each soil type was determined . The soil microbial biomass was determined at the beginning of the test prior to adding the test substance and in test and control soil samples at 4, 6 and 12 months using the fumigation-extraction method . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -23- Test Apparatus and Conditions The test chambers were 250 mL glass serum bottles (Wheaton, Milleville NJ) . The chambers were sealed with butyl rubber septa (Wheaton, Milleville NJ) that were covered with aluminum foil and aluminum crimp caps (Wheaton, Milleville NJ) . The test chambers were identified by project number, test substance ID, test concentration, and unique identifier and incubated statically at approximately 20 2C . Soil Pre-Incubation The soil moisture of each test soil content was adjusted to 40 to 60% of water holding capacity (which is equivalent to a pF of between 2.0 and 2.5, or from 0.1 to 0.33 bar). The test soil then was allowed to warm to the test temperature for at least 2 days. A pre-incubation period of at least 7 days for the aerobic soils and at least 14 days for the anaerobic soils was performed under the appropriate conditions (i.e. temperature, soil moisture content, aerobic or anaerobic). Approximately 25 grams dry weight equivalent of test soil was added to the test chambers. Sterile soil treatments were dosed at approximately 200 mg/kg (dw) with both chloramphenicol (Aldrich, 98%, CAS 56-757) and cycloheximide (Sigma, min. 94%, CAS 66-81-9) to inhibit microbial growth. The chambers to be incubated under aerobic conditions were sealed with aluminum foil-lined septa and incubated. The chambers to be incubated under anaerobic conditions were flushed with anaerobic mixed gas, sealed with aluminum foil-lined septa and incubated. The soil moisture content was maintained in the optimal microbial growth range of 40 to 60% water holding capacity (WHC) . The soil moisture content of each chamber was checked and adjusted, if necessary, once during the pre-incubation period by weighing the test vessels . Sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) was added as necessary to compensate for water losses . Preparation of the Test Chambers The soil moisture content was checked and adjusted, if necessary, prior to the addition of the test substance. Sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) was added as necessary to compensate for water. Anaerobic treatments and controls were prepared within an anaerobic environment and stored in a temperature controlled room . The test substance was applied to the soil within the test chambers as described below. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -24- Backp,round Blank Control : Soils with no test substance added was tested in duplicate to check for background concentrations of analytes (see Sampling and Measurements) . The entire bottle was extracted after a sample of the headspace had been taken. Test Substance : The test substance dosed treatments were prepared by dosing approximately 25 grams (dry weight equivalent) of soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The test chambers were sealed with septum lined with aluminum foil, mixed and incubated . The entire bottle was extracted after a sample ofthe headspace had been taken . Abiotic Controls : The abiotic controls were prepared by dosing approximately 25 grams (dry weight equivalent) of 60Co-sterilized soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The entire bottle was extracted after a sample of the headspace had been taken. SRike Recovery Controls : The spike recovery controls were prepared by dosing approximately 25 grams (dry weight equivalent) of 60 Co-sterilized soil with appropriate volumes of 8-2 FTOH stock (250 pg/mL in ethanol) and fluorinated acid stocks (10 ~tg/mL in water) . The stocks were injected directly into the soil using a glass microsyringe. The test chambers were immediately sealed with septum lined with aluminum foil and the contents of the chambers were mixed. The entire bottle was extracted after a sample ofthe headspace had been taken . Viability Controls (: untreated) The metabolic activity of untreated test soil was assessed on a monthly basis . Duplicate incubation chambers for each test soil and condition (aerobic and anaerobic) were dosed at approximately 100 mg/kg dw with a combination of radiolabeled and non-labelled glucose . The evolved 14C02 (aerobic and anaerobic conditions) and '4CH4 (anaerobic conditions) was measured and the percent biodegradation calculated. Aerobic Controls (treated) : The aerobic controls were prepared by dosing approximately 25 grams (dry weight equivalent) of soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The test chambers were sealed with septum lined with aluminum foil, mixed and incubated. The oxygen content of a sacrificial chamber was measured at monthly intervals. Maintenance of Test Chambers The soil moisture content was maintained in the optimal microbial growth range of 40 to 60% water holding capacity (WHC ; a pF of between 2.0 and 2.5, or from 0.1 to 0.33 bar) . The soil moisture content was initially checked and adjusted, if necessary, at 1 to 2 week intervals by weighing the test vessels but was changed to monthly intervals based on the observed weight losses . Adding sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) compensated for water losses . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 - 25 - The oxygen content in the headspace of two aerobic control chambers was assessed on a monthly basis to ensure aerobic conditions . If the mean measured oxygen content within the control chambers was less than 19%, aerobic test chambers was aerated with filter-sterilized, oil-free air . Test chambers did not have to be opened to add water or for monitoring purposes . Soil moisture content was adjusted by injecting the appropriate amount of sterile water through the septa and onto soil. The headspace oxygen content was measured by inserting the probe of an oxygen analyzer through the septa and into the headspace of the aerobic control chamber. Sampling and Measurements Duplicate chambers from each of the control, treated and spike recovery soils were extracted and analyzed. Sampling intervals were at 0, 1 and 2 weeks and 1, 2, 4, 6, 9 12, 15, 18 and 24 months for aerobic . Potential volatile transformation products in the headspace of the soils chambers were collected using a C18 cartridge. At each sampling time prior to opening the test vessel, the septum was pierced using a needle connected to, a C18 cartridge and syringe. A 20-mL volume of headspace gas from within the chamber was pulled through the C18 cartridge using the attached syringe . The C18 was subsequently extracted with ACN and analyzed. Sample extracts were stored at -10C or lower if analysis is delayed more than 24 hours. Extracts were analyzed for: 1 . 8-2 FTOH 2. 8-2 FTA 3 . 8-2 FTUA 4. PFOA 5 . PFNA 6. PFDA 7. PFUA 8. 7-2 sFTOH 9. 7-3 Acid Quality Criteria Recovery for a given sampling time point should range between 70 to. 120% for the analysis of the 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFNA, PFDA, PFUA, and PFOA from the spike recovery control samples . These ranges should be interpreted as targets and should not be used as criteria for acceptance of the test. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -26- Viability Assessments On a monthly basis, duplicate incubation chambers for each test soil and condition were dosed with 1 mL of the stock solution containing both radiolabelled and non-labelled forms of d-glucose which resulted in a concentration of approximately 3000 mg/kg dw. The headspace of each of the aerobic test chambers was continuously purged at an air-flow rate of approximately 1 bubble per second. The air exiting the test chambers was be passed through a series of gas washing bottles with at least one containing approximately 100 rnL of 1 .5 N KOH . The headspace of each of the anaerobic chambers was continuously purged with a flow of nitrogen and passed through a gas collection system consisting of two sets of COZ traps and a combustion apparatus. The displaced gases initially passed through one empty bottle followed by two bottles each containing approximately 100 mL of 1.5N KOH (COZ trapping solution) followed by another empty bottle . The gas was combined with a flow of oxygen and channeled through a quartz column that was packed with cupric oxide and maintained at approximately 800C in a tube furnace to combust methane to CO2 . The gas exiting the combustion column was passed through an empty bottle followed by two additional COZ traps. The COZ traps connected to the aerobic chambers and the 15` COZ trap in each set (before and after furnace) of the anaerobic mineralization apparatus were removed for analysis and replaced at least weekly. Three replicate 1 mL aliquots of COZ trapping solution were analyzed for radioactivity by liquid scintillation counting (LSC; Packard Tri-Carb Liquid Scintillation Counter). The assessment was terminated when 50% ofthe initial activity was recovered as mineralization products or after 28 days. RESULTS Test Conditions Room temperatures during the acclimation period for all four soils ranged from 18 to 22 C. Temperatures recorded during the test for all four soils ranged from 17 to 25 C. Incubation temperatures outside of the range 18 to 22 C were observed three times over the duration of the study . The frequency of measurements performed on the test soils is presented in Table 1 . The moisture contents of the soils were adjusted as needed based on soil moisture content determinations . Each vessel required water additions at least once during the initial 12-month test period, but no additions were required for shorter incubation times . The maximum amounts ofwater added to any vessel over 12 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -27- and 24 month periods were 1 .2 and 3.9 mL, respectively . The oxygen content in the headspace of the two aerobic control chambers used to assess aerobic conditions is presented in Table 2. Characterization of Soils The test soils were characterized before the start of acclimation, and the results are presented in Table 3. The results of the viability assessments are presented in Tables 4-7 . The results ofmicrobial biomass determinations performed at the start of the test, during the test, and at the end of the test are presented in Table 8. Sample Processing and Analysis Soil chambers were either processed immediately upon receipt or removed from frozen storage and prepared for analysis of the candidate analytes 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA. Headspace Analyses Prior to opening the soil chambers, a 20-mL volume of headspace gas was sampled from each chamber for 8-2 FTOH using a gas-tight syringe (Becton Dickinson, 10 mL with Luer-LokT"' tip), hypodermic needle (Becton Dickinson, 18G 1 %2) and a C,g cartridge (600 mg, Alltech Maxi-Clean) . Each sampled C18 cartridge was extracted with 10 mL of acetonitrile (Burdick & Jackson, HPLC-Grade, CAS No. 75-05-8). The extracts were fortified with 100 gL of a 0.100 mg HDFD/mL internal standard in acetonitrile to yield an internal standard concentration of 100 ng/mL in each extract. The fortifications were performed using a gas-tight syringe (Hamilton 81000 Series). An aliquot of each sample extract was transferred into a glass autosampler vial, sealed and analyzed for 8-2 FTOH by GC/MS. A method outline and typical instrumental conditions for analysis of 8-2 FTOH in the headspace of the soil chambers are provided in Figure 1 and Table 9, respectively. Soil Analyses Following headspace sampling, the crimp seal from each soil chamber was removed and the aluminum foil liner was placed inside the test vessel . A 125-milliliter aliquot of acetonitrile extraction solvent (measured with a graduated cylinder) followed by a 10-milliliter aliquot of 200 mM sodium hydroxide (measured with a serological pipette and prepared with Aldrich, Lot No. 14227T0 NaOH or Fisher, Lot No. 926471 and Burdick & Jackson HPLC-Grade reagent water, CAS Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 - 28 - No. 7732-18-5) were added to each test vessel containing the soil sample . The acetonitrile extraction solvent contained 100 ng/mL of HDFD internal standard and was prepared by fortifying four liters of acetonitrile measured with a graduated cylinder with 400 pL of a 1 .00 mg HDFD/mL primary stock in isopropyl alcohol (Burdick & Jackson, HPLC-Grade, CAS No. 67-63-0) and a gas-tight syringe . The vessels were re-sealed and placed onto a gyratory shaker table (New Brunswick Scientific, Model G10) set for 250 rpm for three hours at room temperature . The vessels were removed from the gyratory table and allowed to settle for several minutes at ambient temperature . Using a volumetric pipette, a 10-mL aliquot ofthe extract from each vessel was transferred into a separate polypropylene centrifuge tube (Nalgene, 50-mL polypropylene Oak Ridge type, Catalog No. 3119-0050) containing 25 milligrams of ENVI-Carb (Supelco, 120/400) . The test vessel, including the remainder of the extract, was replaced into frozen storage (operating range -10 to -20C) . The extract aliquots were centrifuged (Sorvall RC5BPlus fitted with Sorvall SS-34 rotor) for 10 minutes at 12000 rpm (17000 g) and 20 degrees C. An aliquot of supernatant was diluted with reagent water containing 1 .00 ng/mL of 2-13C PFOA internal standard and in the proportions 1 part extract: 3 parts dilution water and the diluted extract was analyzed for 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by HPLC tandem mass spectrometry (LC/MS/MS) . The dilutions were performed directly in HPLC autosampler vials using calibrated micropipettes . The dilution water was freshly prepared by fortifying 100 mL of reagent water with 100 ~tL of a 2-13C PFOA secondary stock in acetonitrile using a borosilicate glass, Class A volumetric flask and either a gas-tight syringe or calibrated micropipettes . A second aliquot of supernatant was diluted with reagent water containing 1 .00 ng/mL of HDFD internal standard and in the proportions 1 part extract: 1 part dilution water and the diluted extract was analyzed for 8-2 FTOH by LC/MS/MS . The dilutions were performed directly in HPLC autosampler vials using calibrated micropipettes. The dilution water was freshly prepared by fortifying 100 mL of reagent water with 100 [tL of a HDFD secondary stock in acetonitrile using a borosilicate glass, Class A volumetric flask and gas-tight syringe. The remainder of the soil extract supernatant was decanted into glass scintillation vials (Fisher, 20-mL disposable, Catalog No . 03-3377) and placed in frozen storage. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -29- At the request of the Sponsor, two additional analytes, 7-2 sFTOH and 7-3 Acid, were screened for in selected stored soil extracts collected prior to the Month 18 sampling interval using LC/MS/MS. The earlier sampling intervals included Day 0 and Months 2, 6, 9, 12 and 15. 7-2 sFTOH and 7-3 Acid were also screened for in soil extracts generated concurrent with the Months 18 and 24 sampling intervals. The soil treatments selected for the screen included one untreated control soil replicate and the live treated soil replicates in each of the four soil types . Three different sample preparation regimes were used. For concurrent analyses of 7-2 sFTOH and 7-3 Acid, an aliquot of soil extract was diluted with reagent water containing 100 ng/mL of HDFD internal standard and 1.00 ng/mL of 2-13C PFOA internal standard in the proportions 1 part extract : 3 parts dilution water. This preparation was used for Month 18 and batch analyses on the earlier sampling intervals, except Day 0. For 7-2 sFTOH analyses only, an aliquot of soil extract was diluted with reagent water containing 1 .00 ng/mL of HDFD internal standard and in the proportion of 1 part extract: 3 part dilution water. For 7-3 Acid analyses only, an aliquot of soil extract was diluted with reagent water containing 1 .00 ng/mL of 2-13C PFOA internal standard and in the proportions 1 part extract: 3 parts dilution water. The latter two preparations were used for processing Day 0 and Month 24 samples where re-analyses due to instrumental or sample stability issues were required (e.g. 7-2 sFTOH stability in the dilutions was suspect). prepared before use . Each of the dilution solvents in reagent water was freshly A method outline for the analysis 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts by LC/MS/MS is presented in Figure 2. Typical instrumental conditions for LC/MS/MS analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA are provided in Table 10. Typical instrumental conditions for LC/MS/MS analysis of 8-2 FTOH are provided in Table 11 . Typical instrumental conditions for LC/MS/MS analysis of 7-2 sFTOH and 7-3 Acid are provided in Table 12. Stock and Calibration Standard Preparation Individual component primary stock solutions (1 .00 mg/mL) were prepared for each analyte and internal standard from the neat materials using an analytical balance and volumetric glassware. Individual component secondary stock solutions (100, 10 .0, 1 .00 and 0.100 gg/mL) were also prepared by serial dilution. The 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 2-13C PFOA and HDFD (for 24 month interval only) primary stock solutions were prepared in acetonitrile . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -30- The 8-2 FTOH, 7-2 sFTOH and HDFD (except as noted for the 24 month interval) primary stock solutions were prepared in isopropyl alcohol . All secondary stocks were serially diluted with acetonitrile . The 100-~ig/mL HDFD secondary stock solution was used to fortify the internal standard into soil extraction solvent, calibration standards containing 8-2 FTOH and 7-2 sFTOH and dilution solvents for analyses of soil extracts for these analytes. For GC/MS analyses of 8-2 FTOH in the headspace gas of soil test chambers, 8-2 FTOH calibration standards in acetonitrile (25 .0, 50.0, 100, 250 and 500 ng 8-2 FTOH/mL) and each fortified at 100 Vtg HDFD/mL internal standard, were prepared from the appropriate individual component stocks . Representative ion chromatograms for 8-2 FTOH calibration standards by GC/MS are presented in Figures 3 and 4. For LC/MS/MS analyses of 8-2 FTOH in soil extracts, 8-2 FTOH calibration standards in 50: 50 (v/v) acetonitrile : reagent water (10 .0, 25 .0, 50.0, 100, 250 500 and 1000 ng 8-2 FTOH/mL) and each fortified at 100 ~Lg HDFD/mL internal standard, were prepared from the appropriate individual component stocks . Representative ion chromatograms for 8-2 FTOH calibration standards by LC/MS/MS are presented in Figures 5 and 6. For 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA analyses of soil extracts, a 100~tg/mL -combined stock of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in acetonitrile was prepared from the individual component primary stocks . Secondary combined acid stocks in acetonitrile (10 .0, 1 .00 and 0.100 ~tg/mL) were prepared by serial dilution from the 100 jig/mL combined stock solution. Combined 8-2 FTA; 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standards in 25: 75 (v/v) acetonitrile : reagent water (0.100, 0.250, 0.500, 0.750 and 1 .00 ng/mL) and each fortified with 0.75 ng 2-13C PFOA/mL internal standard were prepared from the 0.100-gg/mL combined stock. A 5 .00 ng/mL combined 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standard in 25 : 75 (v/v) acetonitrile : reagent water was prepared from the 1 .009g/mL combined stock. All stock and calibration standards were stored under refrigerated (-4 degrees C) conditions when not in use . For 7-3 Acid and 7-2 sFTOH analyses of Months 2, 6, 9, 12, 15, 18 and 24 (7-3 Acid only) soil extracts, two sets of combined stocks were prepared . A 10-pg/mL combined stock of 8-2 FTA, Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -31- 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in acetonitrile was prepared from the individual component primary stocks . Secondary combined stocks in acetonitrile (1 .00 and 0.100 pg/mL) were prepared by serial dilution from the 10.0-4g/mL combined stock. A 10.0-gg/mL combined stock of 8-2 FTOH and 7-2 sFTOH in acetonitrile was prepared from the individual component primary stocks . Calibration standards in 25 : 75 (v/v) acetonitrile : reagent water containing 0.75 ng 2-13C PFOA/mL and 100 ng HDFD/mL internal standards were prepared from the 0.100 and 1 .00-~tg/mL combined perfluorinated carboxylic acid (PFCA) and 10.0-gg/mL combined telomer alcohol stock solutions, respectively . The nominal concentrations of7-3 Acid and 7-2 sFTOH in the calibration standard set (7-3 Acid/7-2 sFTOH) were 0.100/5 .00, 0.250/10 .0, 0.500/25 .0, 0.750/35 .0, 1 .00/50 .0 and 5 .00/250 ng/mL. Due to stability concerns, fresh preparations of 7-2 sFTOH calibration standards were prepared from the 10.0-gg/mL combined stock of 8-2 FTOH and 7-2 sFTOH in acetonitrile for analyses of 7-2 sFTOH in Month 24 soil extracts. The nominal concentrations of 7-2 sFTOH were as specified above. For 7-3 Acid analyses of Day 0 soil extracts, a combined calibration standard for set for 7-3 Acid and 7-2 sFTOH only was prepared as described above from individual component 7-3 Acid (0.100 and 1 .00-ng/mL) and 7-2 sFTOH (10 .0-ng/mL) secondary stock solutions . Only 7-3 Acid results were reported from this standard set due to a delay in instrumental analyses. For 7-2 sFTOH analyses, another set of standards was freshly prepared from the 10.0-gg/mL 7-2 sFTOH secondary stock solution. Representative ion chromatograms for 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standards by LC/MS/MS are presented in Figures 7-22. Calibration Curves and Limits of Quantitation Calibration standard sets for a given analysis were injected at the beginning and end of each analytical sequence with one calibration standard injected after a maximum of every five study samples. For 8-2 FTOH analyses in the headspace of test vessels by GC/MS, linear regression equations were generated using adjusted peak areas (for the internal standard response ratio) versus the respective concentration of the calibration standards. The concentration of 8-2 FTOH in the sampled headspace of the soil test vessels was determined by substituting the peak area response ratios obtained for the samples into the applicable linear regression equation . The concentrations were converted to a mass basis using the appropriate volumes, weights and unit conversion factors . A representative calibration curve for 8-2 FTOH analyses by GC/MS is presented in Figure 23 . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -32- For soil extract analyses of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA,7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS, weighted linear regression equations were generated using peak area response ratios (analyte:intemal standard) versus the respective concentration ratios (analyte :internal standard) of the calibration standards . The concentration of each analyte in the samples was determined by substituting the peak area response ratios obtained for the samples into the applicable linear regression equation. Representative calibration curves for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA analyses by LC/MS/MS are presented in Figures 24-32, respectively. The limits of quantitation (LOQ) for these analyses were defined on a low-level standard equivalent basis and were calculated for 8-2 FTOH in the sampled headspace and for each analyte in soil as the product of the low-level standard concentration and the dilution factor of the untreated control samples . The LOQ for 8-2 FTOH in a 20 mL headspace gas aliquot was 12.5 ng/mL sampled headspace gas, or 0.250 pg for a 20-mL sample . The LOQ was 2.0 pg/kg for soil analyses of 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA. The LOQs for soil analyses of 8-2 FTOH and 7-2 sFTOH were 100 and 100 gg/kg, respectively. Statistical derivation of the limits of detection (LOD) was completed for all analytes with the exception of 7-3 Acid and 7-2 sFTOH during method verification trials . The calculation for each analyte was based on three times the concentration equivalent of the standard deviation of five replicate analyses of a reagent blank. Method verification trials and derivation of statistical LODs for 7-3 Acid and 7-2 sFTOH was outside the scope of the study. Measured concentrations of the analytes are presented in mg/kg soil dry weight and pmol/kg soil dry weight in Appendix I and II, respectively. Measured concentrations of 7-2 sFTOH and 7-3 Acid in the untreated soils were less than the LOQ and are not presented . Example calculations are presented in Appendix III. Representative chromatograms are presented for each of the four treatment groups in aerobic alfisol soil. Similar chromatographic characteristics were evident for the aerobic ultisol, mollisol and inceptisol soils. Representative ion chromatograms for 8-2 FTOH by GC/MS in the sampled headspace of untreated control, fortified control, sterile and live samples treated with[ ~ from Day 0 of the test are presented in Figures 33, 53, 69 and 85, respectively. Representative ion chromatograms for Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -33- 8-2 FTOH in the sampled headspace of untreated control and live samples treated withC - Month 24 of the test are presented in Figures 43 and 95, respectively. -~from Representative ion chromatograms for 8-2 FTOH by GGMS in the sampled headspace of fortified control and sterile samples treated withC ~ from Month 12 of the test are presented in Figures 61 and 77, respectively . Representative ion chromatograms for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from untreated control samples from Day 0 and Month 24 of the test are presented in Figures 34-42 and 44-52, respectively . Representative ion chromatograms for 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from fortified control samples from Day 0 and Month 12 of the test are presented in Figures 54-60 and 62-68, respectively. Representative ion chromatograms for 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from sterile samples treated with L Month 12 of the test are presented in Figures 70-76 and 78-84, respectively . ,from Day 0 and Representative ion chromatograms for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from live samples treated withL jfrom Day 0 and Month 24 of the test are presented in Figures 86-94 and 96-104, respectively. Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. -34REFERENCE Project No.: 112E-108 1 Organisation for Economic Cooperation and Development. April 2002. Aerobic and Anaerobic Transformation in Soil. OECD Guideline 307. p. 36 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -35 - Table 1 Frequency of Measurements' Interval Biomass Soil moisture Activity Headspace oxygen Soil metabolic Analytical interval content activity Day 0 Week 1 Week 2 Week 4 Week 6 Month 2 Month 3 Month 4 Month 5 Month 6 Month 7 Month 8 Month 9 Month 10 Month 11 Month 12 Month 15 Month 18 Month 21 Month 23 Month 24 'Additional chambers for the measurement of biomass and headspace oxygen content beyond the initial 12 month test period were not prepared at the start of the study . p. 37 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Interval Month 1 Month 2 Month 3 Month 4 Month 5 Month 6 Month 7 Month 8 Month 9 Month 10 Month 11 Month 12 -36- Table 2 Test Chamber Headspace Oxygen Content Alfisol 20.8 21 .0 20.9 20.8 20.8 20.9 20.8 20.8 20.8 20.9 20.8 20.9 Avera e Ox Ince tisol 20.8 20.9 20.9 20.8 20.7 20.9 20.7 20.8 20.8 20.9 20.8 20.9 en Content % Mollisol 20.7 20.7 20.6 20.8 20.7 20.7 20.8 20.9 20.8 20.8 20.8 20.8 Project No.: 112E-108 Ultisol 20.8 ' 20.7 20.6 20.8 20.8 20.8 20.7 r 20.8 20.8 20.9 20.8 20.8 p. 38 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Proj ect No. : 112E-108 Soil label Texture class Alfisol Inceptsol Ultisol Mollisol Sandy loam Loam Sandy loam Sandy clay loam 1- cation exchange capacity 2- H in 1 :1 soil water ratio -37Table 3 Selected Soil Properties Organic carbon content % 1 .4 10 2.9 2.1 Sand Silt Clay % 65 24 11 45 32 23 55 31 14 59 18 23 CEC' meq/100 g 7.8 16 .9 7.9 17 .7 Water holding capacity pH2 % 5.8 54 .5 6.6 43 .3 4.8 59 .3 6 .9 63 .2 Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -38- Table 4 Soil Viability by Glucose Mineralization: Alfisol Month Glucose Mineralization Rate Aerobic An_ aerobic 1 1 .83 1 .81 2 1 .79 2.68 3 2.45 7.34 4 2 .73 7.36 5 2 .54 4.17 6 2.60 3.00 7 3.99 NA 8 3.79 NA 9 7.57 NA 10 2.64 NA 11 2.39 NA 12 2 .00 NA 15 1.64 NA 18 1.81 NA , 24 1 .81 NA - (percent biodegradation/elapsed time in days) x 100 Table 5 Soil Viability by Glucose Mineralization: Inceptisol Month Glucose Mineralization Rate' Aerobic Anaerobic 1 2.48 1.95 2 4.15 3.89 3 8.60 8.44 4 8.90 7.76 5 8.29 3 .34 6 8.97 3 .02 7 9.50 NA 8 9.47 NA 9 10.46 NA 10 8.91 NA 11 8.50 NA 12 8.04 NA 15 8.14 NA 18 8.06 NA 24 8.90 NA - (percent biodegradation/elapsed time in days) x 100 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -39- Table 6 Soil Viability by Glucose Mineralization: Ultisol Month Glucose Mineralization Rate' Aerobic Anaerobic 1 2.23 2.22 2 2.56 2.39 3 1.67 2.86 4 1 .95 4.22 5 4 .04 5.41 6 2.46 3.80 7 3 .79 NA 8 7.33 NA 9 7.37 NA 10 8.83 NA 11 2.99 NA 12 3.61 NA 15 8.19 NA 18 2.37 NA 24 3 .65 NA '- (percent biodegradation/elapsed time in days) x 100 Table 7 Soil Viability by Glucose Mineralization: Mollisol Month Glucose Mineralization Rate' Aerobic Anaerobic 1 2.36 1 .85 2 2.54 2.99 3 1 .72 2.57 4 3.63 3.99 5 4.06 4.82 6 3 .66 4.16 7 3 .74 NA 8 3 .95 NA 9 3 .60 NA 10 7.51 NA 11 2.73 NA 12 1 .78 NA 15 3 .59 NA 18 2.11 NA 24 1 .81 NA '- (percent biodegradation/elapsed time in days) x 100 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -40- Table 8 Soil Microbial Biomass (n/g) (ISO 14240-2 ; fumigation-extraction) ALFISOL Aerobic Anaerobic Treated Untreated Treated Untreated Post Inc . - 244 - 128 Month 4 317 323 435 287 Month 6 680 279 593 645 Month 12 769 675 NA NA NA - Not analyzed INCEPTISOL Aerobic Anaerobic Treated Untreated Treated Untreated - 131 - 132 138 109 184 106 432 483 423 467 504 564 NA NA Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. Project No. : 112E-108 -41- Table 8 (continued) Soil Microbial Biomass (~tg/g) (ISO 14240-2; fumigation-extraction) Post, Inc. Month 4 Month 6 Month 12 MOLLISOL Aerobic Anaerobic Treated Untreated Treated Untreated - 190 - 53 216 218 218 205 243 259 267 270 714 734 NA NA ULTISOL Aerobic Anaerobic Treated Untreated Treated Untreated - 312 - 54 36 31 26 34 486 194 500 407 565 570 NA NA NA - Not analyzed Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -42Table 9 Typical GC/MS Operational Parameters for Analysis of 8-2 FTOH in Headspace Gas Instrument: Detector: Analytical Column : Injector Temperature: Oven Temperature Profile : Hewlett-Packard Model 5890 Series II Gas Chromatograph (GC) Hewlett-Packard Model 5971 Mass Selective Detector Phenomenex Zebron ZB-5 (30 m x 0.25 mm, 0.25-1,un film thickness) . 150C Initial temperature : 50C Initial hold time: 2.00 minute Ramp: Rate (C/minute) 20.00 50.00 Final Temnerature (0 100 300 Detector Temperature : Carrier Gas : Septum Purge: Split Vent: Injection Volume : Data Acquisition Mode : Acquisition Ions: Peak Retention Times : 8-2 FTOH: HDFD: Final hold time: 3.00 minute 280C Helium, -l mL/min ; CHP = -14 psi Helium, -5 mL/min -40 mL/min 2 [tL (2 stops w/ 10 111, syringe), splitless SIM Component Quantitation 8-2 FTOH 31 m/z HDFD 33 m/z Approximately 5.0 minutes Approximately 5.0 minutes Confirmation 95 m/z 98 m/z p. 44 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -43Table 10 Typical LC/MS/NIS Operational Parameters for Analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in Soil Extracts Instrument: Analytical Column : Column Temperature: Mobile Phase: Gradient Elution Profile : Injection Volume : Monitored Ion Transitions : PFOA 2-13 PFOA 8-2 FTA 8-2 FTUA PFNA PFDA PFUA (internal standard) LC/MS/MS Analog Parameters : Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source . Operated in the negative ion multiple reaction-monitoring mode . Zorbax RX-C8 (150 mm x 2.1 mm, 3-~tm particle size) 40C A: 0.1% Formic Acid in Reagent Water B : Acetonitrile Time (min) 0.00 1 .00 8.00 9.00 9.10 14 .00 15 .00 20 .00 Flow Rate (gL/min) 250 250 250 250 250 250 250 250 Percent A 70 .0 70 .0 15 .0 15 .0 5.0 5.0 70 .0 70 .0 Percent B 30 .0 30 .0 85 .0 85 .0 95 .0 95 .0 30 .0 30 .0 50 .0 gL Q1 413 .0 amu 415 .0 amu 477.0 amu 457.0 amu 463.0 amu 513.0 amu 563.0 amu Q3 369.0 amu 370.0 amu 393.0 amu 393.0 amu 419.0 amu 469.0 amu 519.0 amu Dwell Time 250 msec 250 msec 250 msec 250 msec 250 msec 250 msec 250 msec Retention Time 9.82 min 9.82 min 12.2 min 12 .3 min 10 .6 min 11 .2 min 11 .8 min IS =4500 TEM = 400 DP=-10 FP=-140 EP = -10 CE _ -15 CXP = -25 CUR =8 CAD =4 NEB = 12 p. 45 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -44Table 11 Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTOH in Soil Extracts Instrument: Analytical Column : Guard Column : Column Temperature: Mobile Phase: Isocratic Profile: Injection Volume : Monitored Ion Transitions : 8-2 FTOH HDFD (internal standard) LC/MS/MS Analog Parameters : Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source. Operated in the negative ion multiple reaction-monitoring mode . THERMO EC Betasil C-18 (50 mm x 2.1 mm, 5-4m particle size) Javelin Betasil C-18 (20 mm x 2.1 mm) 40C A: 0.15% Acetic Acid in Reagent Water B: Acetonitrile Time min 0.00 6.00 Flow Rate (pUmin) 250 250 Percent A 30 .0 30 .0 Percent B 70 .0 70 .0 25 .0 pL Q1 523 .1 amu 528 .1 amu IS = -4500 TEM = 450 DP = -25 FP = -250 EP = -10 Q3 58 .9 amu 58 .9 amu Dwell Time 500 msec 500 msec CE = -50 CXP = -10 NEB = 12 CUR = 7 CAD = 6 Retention Time 4.18 min 4.15 min Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -45Table 12 Typical LC/MS/MS Operational Parameters for Analysis of 7-3 Acid and 7-2 sFTOH in Soil Extracts Instrument : Analytical Column: Column Temperature: Mobile Phase: Gradient Elution Profile: Injection Volume : Reported Ion Transitions : 7-3 Acid 2-13 PFOA (internal standard) 7-2 sFTOH HDFD (internal standard) LC/MS/MS Analog Parameters : Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source. Operated in the negative ion multiple reaction-monitoring mode . Zorbax RX-C8 (150 mm x 2.1 mm, 3-Rm particle size) 40C A: 0.15% Acetic Acid in Reagent Water B: Acetonitrile/ 0.15% Acetic Acid Time (min) 0.00 1 .00 1 .01 7.00 12 .00 13 .00 20 .00 Flow Rate (pL/min) 250 250 250 250 250 250 250 Percent A 95 .0 95 .0 70 .0 15 .0 15 .0 95 .0 95 .0 Percent B 5.0 50 .0 30 .0 85 .0 85 .0 5.0 5.0 50 .0 gL Q1 441 .0 amu 415 .0 amu 473 .0 amu 528 .0 amu Q3 337 .0 amu 370.0 amu 59 .0 amu 59 .0 amu Dwell Time 50 msec 50 msec 50 msec 50 msec Retention Time 11 .6 min 10 .0 min 11 .9 min 12 .1 min IS = -4500 TEM = 400 EP = -10 CUR = 7 CAD = 6 NEB = 132 DP = FP = CE _ CXP = 7-3 Acid/ 2-13 PFOA -40 -350 -15 -11 7-2 sFTOH/ HDFD -25 -250 -50 -10 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -46- Project No.: 112E-108 Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers For each sample, fit a Cl8 cartridge between the end of an appropriate volume syringe and a hypodermic needle. With the plunger fully depressed, insert the needle of the disposable syringe through the septum of the sample and pull a full syringe volume . Remove needle, expel headspace air and repeat. Detach CI8 cartridge and place in appropriate holder. Attach the barrel of a 10 mL disposable syringe to CIg cartridge and use a volumetric pipette to transfer acetonitrile extraction solvent into the syringe barrel . Insert the syringe plunger into the barrel and inject the acetonitrile through the C,8 cartridge into a 20-cc glass scintillation vial. Fortify the extract to contain 100 ng/mL 1D, 1D, 2D, 2D, 3-13C Heptadecafluoro Decanol internal standard. Pipette an aliquot of the extract directly into an autosampler vial. Cap vial and submit for GC/MS analysis of 8-2 FTOH. All sample vials not analyzed immediately are place in refrigerated storage . Store the remaining trap extract in scintillation vials under frozen conditions. Figure 1 . Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -47- Project No. : 112E-108 Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts For each sample vessel, remove crimp seal and place foil liner into the vessel. Using a graduated cylinder, measure and transfer 125 milliliters of extraction solvent into each sample vessel . The extraction solvent consists of 100 ng/mL 113, 1D, 2D, 213, 3-13C Heptadecafluoro Decanol internal standard in acetonitrile . Add a 10-mL aliquot of200 mM NaOH into each sample . Re-crimp each sample vessel with a new aluminum crimp cap and the original septum. Extract the samples for approximately three hours using a gyratory shaker table set for 250 rpm . 1 Allow suspended material in extracts to settle . Remove a 10-mL aliquot ofthe extract using a serological pipette and transfer into a separate 50-mL plastic centrifuge tube preloaded with approximately 25 mg of Envi-CarbTM. Centrifuge samples at a setting of 12000 rpm for 10 minutes . Transfer extract supernatant for each sample into a separate labeled scintillation vial. For 8-2 FTOH analyses, pipette an aliquot of the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 100 ng/mL 1D, 1D, 2D, 2D, 3-13C Heptadecafluoro Decanol (HDFD) internal standard to produce a final solution composition of 1 :1 (v/v) extract: water. For 7-2 sFTOH analyses, pipette an aliquot of the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 100 ng/mL HDFD to produce a final solution composition of 1 :3 (v/v) extract:water . Perform dilution directly in an autosampler vial, cap and vortex. Submit for HPLC/MS/MS analysis of telomer alcohols . All sample vials not analyzed immediately, place in refrigerated storage . For PFCA analyses of each sample, dilute an appropriate volume of the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 1 .00 ng/mL N-Perfluorooctanoic Acid, [1,2 di13C] internal standard to produce a final solution composition of 1 :3 extract : water. Perform dilution directly in an autosampler vial, cap and vortex. Submit for HPLC/MS/MS analysis ofPFCAs . All sample vials not analyzed immediately, place in refrigerated storage . Re-crimp each sample vessel with a new aluminum crimp cap and the original septum. Store sample vessels and extract scintillation vials under frozen conditions. Figure 2. Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts . p. 49 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -48- Wildlife International, Ltd. GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : T :\DATA\ALFI025\0101005 DW 20 Apr 106 5 :41 pm GCMS#1 TA-30 25 .0 ug I .D a .i ./L IAbundance 1500 Ion 31 .00 (30 .70 to 31 .70) : OlO1005 .D Abundance 250 200 150 Time-->4 .00 Ion 95 .00 (94 .70 to 95 .70) : OlO1005 .D S .SO2 Figure 3. Representative ion chromatograms for 8-2 FTOH in a low-level (25 .0 ng a.i./mL) calibration standard by GC/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No .: 112E-108 -49- Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : T :\DATA\ALFI025\0501009 .D DW 20 Apr 106 7 :03 pm GCMS#1 TA-34 500 ug a .i ./L 5 Abundance Ion 31 .00 (30 .70 to 31 .70) : 0501009 .D 4 .199 Time-->4 .00 IAbundance i 3000 1 4 .20 4 .40 4 .60 4 .80 Ion 95 .00 (94 .70 to 5 .00 5 .20 5 .40 95 .70) : 0501009 .D 5~00 5 .60 2000- 1000 - Irime-->4 .00 4 .20 4 .40 4 .60 4 .80 5 .00 5 .20 5 .40 5 .60 Figure 4. Representative ion chromatograms for 8-2 FTOH in a high-level (500 ng a.i./mL) calibration standard by GC/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -50- p. 51 Project No.: 112E-108 Figure 5 . Representative ion chromatogram for 8-2 FTOH in a low-level (10 .0 ng a.i./mL) calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -51 - Project No.: 112E-108 Figure 6. Representative ion chromatogram for 8-2 FTOH in a high-level (1000 ng a.i./mL) calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. -52- Project No. : 112E-108 ......,.. .... . ... '_- . i ---~-T----~--7 'T aa Figure 7. Representative ion chromatograms for 7-2 sFTOH in a low-level (5.00 ng a.i./mL) calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -53- p. 54 Project No. : 112E-108 ~~ ,--I -I -- -s I._ Figure 8. Representative ion chromatograms for 7-2 sFTOH in a high-level (250 ng a.i./mL) calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 ~.~.: amam,- s 'RskNin:.'07~' kY~(u) "97ACNdOmJ cvmiK'IGASC MiM.m:' s~plt I 6~plc ryp: ~ sciva .aa o vg ~.i ./L Caleula.a Cmt. o cHa]f ug t. i ./tg ~. TS_: of/e/oooix xcditi W: Yr c F+ve~alggsichs: Wlyc[ duac c iTtuhWl: Thaah eia : x . acxb : s.. . xiuon.: m~. e. a ~ 31 .11 a cp. ep ao.~c .a tm : k .l~tiv. tr: xe es~~x..: ' Y~t =112_1 c c `PS 3Y . ~ m~n -54- 90m Ss~ StW 5307 sam cem .em a~m am 7!D) 7C07 310) - 120 - ]oy 3Gm w 7s0 blm 3Z0 ' OD) ,Om 7!m ta~ 710 ,I36 nr o ema~ 7yY tom "~ nv a nsu 7 x s . s s 7 e a n n tz ta w ts s n ts ,c Figure 9. Representative ion chromatogram for 8-2 FTA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -55- Project No .: 112E-108 AlkN.n" '62;)' ~Cmma'IL?6E' t~pl. S:d.c : _MMDCiiOmJ /nM"im~ 77 ~cVVU nPim . ~eullt.d [mc: Tcq_ D.t.: Tcq. Tas. 7~Saa~ i./L a !Si! u .a ./k 05/11/70055 ' 05 .5 . .50 !x xrd1li W: Pmc. 711griel. : s: . :tv~eia ; rn..wla : s ~an.: ss~y.. xx S._aSSknec: : s:. . ! 1: w' v :~ : nq.~lca.a.u.: kx: 1D..lya ! x Qu .ic e! a .:+a 70 o TmTa xaec : e:S i1 T.i..:.: 7 t"t~c. 17 .! d 9 .1N 711M 2yw 2sw v.~ xa .+ zsr z~r zt.t xzr z,.o zDw +w +7 .a - t8N 1225 tYt 1Lt 1 .1 W 1M LOm. GOm. 70m. lOm. 600). ~Om. ]OD). 1~. DD + 2 5 ~ s s ~ s s ax u n +s +s n +s n Figure 10. Representative ion chromatogram for 8-2 FTA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -56- Project No. : 112E-108 1 ~ . ,.., Figure 11 . Representative ion chromatogram for 7-3 Acid in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -57- Project No.: 112E-108 ., Figure 12. Representative ion chromatogram for 7-3 Acid in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. -58- Project No.: 112E-108 anp.:rr:.: ampe_wi a~ru . a:uu.iw:pv 'M4N:n.'b2N7 NWrI~9NA3a .m7 Cmm.:['ILl4E' hn6"im:' prpl. Ivnc : : mlr e 7ype . im : :WN ug . .i ./L c.leul.W cm Aty. a.n . as . a up ..i ./tp 00/70~/7000~ Os . : 0 llatifi.d : Aoanc ~ :e,oeiessnie~: TeceXOla: ~TE_n.EOld : 1 : el.. .i~ op. p s~ y. pXi"t1,tN e~. s wcio: TW ~g ~i.`i:c:aax : ype niimxir : p ~ s ~:e : s rf.. : rs. .~ xe wmin as ui~ u.s . .n rr . w xii:nurtw - SM1 44AA.4 42" aaw sew ss.~ sa .~ 33M 3aM 31ee 26M n 3NA 33M 2At 12M 16N 1A .! 12 .4 tA1 nOD). n0m. "00). 30m. oa 7 3 s s s v n . . .. 1270 n tt c r 15 ts n tn u Figure 13. Representative ion chromatogram for 8-2 FTUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -59- yeO.Rme:amP_waauR.u:-a~uem:WC- c. :wTms~a:CU,Itws- ' f~ekN~r.i 47NT ~y~l'"9NA]Deml ConnK'tL?6H" Inp"'vr.' . 8~7~ ]ea . '71T :, cpr.vl~cHCm<: " cq . D Aaq. rau: Suvd~ca ^9 f uy oi%ii% 7ooe o".se .se in ~a ./b ".a ./t9 2~ 7TK neaisi .a : nma : 7 "ml~g:ricns : Yulyec i:ms TR.eneld~ _ ~fin ae : ~ fR . 8 .19hc : iR . Pu7t Y ~ eR~. V~1. o . clu~ic inaav: ~4 1acS wc0.h Fo ~e tW5 as 2Fe5 33 " 5 sa .o vc Ib5 tm a T' l~iync : =I T, r~ w1i"l 7e ~'ccp~ an .7 un 21"s 3is -5 1bs 7]a5 165 - 75e5 tI"5 13e5 1 1. ' 1De5 YINI eD." fD.1 snw snr sae zw" l~e oD i a s a s s T s e v ------ - ----- . .- -. , 1270 . ., ' , 4 u is es n ls ~a Figure 14. Representative ion chromatogram for 8-2 FTUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -60- Project No.: 112E-108 3 Figure 15 . Representative ion chromatogram for PFOA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not .contain TSCA CBI . Wildlife International, Ltd. -61- _.;, o_p ,..E.. Project No.: 112E-108 _ ;.., Figure 16. Representative ion chromatogram for PFOA in a high-level (5 .00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. p. 63 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -62- Project No. : 112E-108 axkwm.MY:axx'MS(e7'"sn"tuo .n,:r co~.wticnste- a.u~o,e- =ci . r c - )~ eutaact[cnc 'cp 7Im.. Oo ug ave a'700iw9 o_ .of o = ./t .t ./kp - - nalya Clamm .c mm~c]d .~ 1 -pe e. Usa F.Ltavm FT, tb indw : -o .o S.OK~ ",~.F "AM mmc 02W " OK ]~ ] .6e0 ] "M 37m0 ]M1 2dK G 7.6M I" .o . 33M 2Oea1BM16M" 1 "N12M1oM~ 90W.0 9000.0 "N0.0 10000 0.0 3 4 5 fT i ! H 70 11 R 11 14 11 16 __ . TXro.IMn Figure 17. Representative ion chromatogram for PFNA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -63 - Project No. : 112E-108 ' fanM 7LMt7T .41. 1 - 4m- _' -_ _ ciyi e. _ :.i>, .+ .i~ a ,... oa . .._a aN ., -v _ v1 . p =. e~eQ .cfve ei . No - sln . w on 7a .d .tmv B -+ 11 .1 mu. _o .o aas zws xas 28e5 2 Sa5 045 R~.S 2~~ 21.5 t .p5 1 NS +7S r tA5 i~ 1 .SC - f .aS 7aa tb5 nn 1os sa.. tor s.o .~ s o .a . a.f aa+ Z a~ ao +o .n , a . s s v s a lo n +2 + . 7 _ +s +e+sis Figure 18. Representative ion chromatogram for PFNA in a high-level (5 .00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. p. 65 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -64- vrrwm..o,oArm' Wwc.q-3,3w~su.a .nv~ oom~ .n ,.aa-,e.a . .Y: rwoum- .mw~il". x~x St . d.rd - ua~c~~- - - o<i :vzu Alpcrlchm : ~rnc..n_ia~ hcla : wlac-enc: h.: I". " . cl.u..c xs v.i . ~ ~. .. :*.ac'...,ve..t:I..T,: IT, _ _ s .o.x 3esa SBN 5.<K S1M so., e s,a ~ s .a aw a2,a a .aa 3AN 3.lN ' 3AN = 32M ~G 3Ae6 2.~ 2AM R/M 2 .]a 2 .OM 78M 18.7 1 AK tM 1At fi000.0 lOODA ~OOD 0 MW.O 00 - Project No.: 112E-108 Figure 19. Representative ion chromatogram for PFDA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -65 - Project No.: 112E-108 ~~R:z,a ~ 1 com:wT.ursa' w.n+~R- ..Fi. z ~ii ..ala .. _ _ o.iai~oes- . . . 3 _ _ __ .smr..nma : - ..,:7a : ]c Wak~R .00 e. e Re1 .cRVV.c.0S . c . Typ.: m T 3 Nv Ftn - _- ro . Ti.e . .a .a .m 7,s 7as _c .o 3y5 3rs :ss rc 3Se5 2".5 3].5 7M 3,.5 2M5 ,AS 1H5 ~: 1>b 6 i3 , .t .5 t 1 SaS ,s5 n , as cw eos 7 .ow e.a.c SM "AK 7.ba 30 .c n 3 0 ~ 5 6 7 ! Y ,0 ,7 77 75 16 17 7 1! P Figure 20. Representative ion chromatogram for PFDA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife Intemational, Ltd. -66- RoeW,f.Yn~Knu3Ls1-,,Ier-b-n. Mrla1'-SCi .auai-- .Q'376A mpl. , .a- . ./~ oi/11~700f -d~r1W, - - dlgezichn: A :ulyec CLevac held~ 2- 4~ -p ~rn*..n_la : ix v.i . re: o . _ .oo SOw 6BM <ew- ~ eaz.o..: ~ow~ aew 9ax s~w S .zw a aa zew ze.a z2 . 2-- p. 67 Project No .: 112E-108 , ae eaoo.o eaoo 00 -o m3o. o.o ,a tz 1e 17 ,e ta Figure 21 . Representative ion chromatogram for PFUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -67- Project No.: 112E-108 ~~: *~9._ 9~:~.=x ..,~ - u:; ~i ;` uuw ~s_. .sn m nwata.d : - x16=zvcl:m . -- _ Ar:+ly.c ~l.. .ac - _ =7- cF xx7k R .1 . R a. e Ra.uve R7 . xe on iv val.y 79 ivme . =7 .t mm m. ' man _c .e T8.5 T7.5 38.5 T3.5 3N5 vc 2].5 2~ 31.5 2.M 'W 11.5 17 S , 6 .5 S 1 .15 1~ .5 12.5 11 .5 1.0.5 9A1 eow 70.. sow 50N ~ M. TAN 1 AIM 00 , 3 ] e e 7 e ,0 1, ,3 1] 14 te 16 ,7 le t Y Figure 22. Representative ion chromatogram for PFUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -68- Project No.: 112E-108 700000 w 600000 0 500000 - d 400000 x a 300000 0 200000 100000 0' 0 ; -- 100 200 300 400 500 600 Concentration (gg 8-2 FTOH/L) Figure 23 . Representative calibration curve for 8-2 FTOH by GGMS. p. 70 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -69- Project No.: 112E-108 042406 MI2 s3ara>(a-2 Frai):-uneY ftmssicnP1 1x vos"t y=1 z x " o .ooB29(r=0.sese) 12 .011 .5- 11 .0 . 10.5 / 10.0 9.5 90 ~% fl5 ao 7.5 7.0 / &5 6.0- ////~~~ S5- 5.0- / 4.5- / 40 35 , j 3.0- J_ Z& 20 1. 1 .0 r QS ~, 0.0 1 2 3 4 5 6 7 8 9 10 hmai~te Cac / IS Cam Figure 24. Representative calibration curve for 8-2 FTOH by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -70- p. 71 Project No. : 112E-108 052207_M24S34-TaTAL.nib (7-2 FTOH): "tineaP Regreaion ("1 /x"weightin9): Y' 1.91 x r-O,p272 (r= 0.9986) 5.0 48 4.6 4A 42 4.0 3.8, 35 3A 32 3.0 2.8 26 2A 22 2.0 1.81.6 . 1A 12 1.0 0.80.6, 0.4 02 - 0_1 y' '' % " // ' ~ ~ . ~l 02 03 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1 1'2 113 1.4 1.5 1.6 1.7 1.8 1 .9 20 21 22 23 2.4 2.5 Analyle Conc. / IS Conc. Figure 25. Representative calibration curve for 7-2 sFTOH by LC/MS/MS . p. 72 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -71- Project No.: 112E-108 ' 042006 M12 TELALAE.rdb (8-2 (S)) : "Linear" Regression ("1 / x" weigMlng) : y=0 .116 x+0.00123 (r= 0 .9992) 0 .80 - 0 .75 - 0 .70 0 .65 - i 0.60 - i ' 0.55 D 0 .50 < m 0 .45- D m 0 .40- m 0 .35 i % / D 0.30 - m 0.25 - 0.20 7 0 .15 0 .10 - / ~` / j i / f~ 0 .05 0 . 00 - 0 .5 1 .0 1 .5 2.0 2 .5 3 .0 3 .5 4 .0 4 .5 5.0 5 .5 6.0 Analy6e Conc . l IS Conc . f 6.5 Figure 26. Representative calibration curve for 8-2 FTA by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -72- Project No. : 112E-108 " 1~ENl5SiZ#~(73F~y1rm"F~~('1/~Yve~Tr~ 30 25 r`f / ! 20 15 " . 05 ` / / _ .. . . , .... 05 t0 15 20 25 30 35 40 45 50 55 60 65 Lt~~/19(b~ Figure 27. Representative calibration curve for 7-3 Acid by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -73- Project No.: 112E-108 042006 M12 TELALAE.rdb (8-2 (U)) : "Linea' Regression ("1 /)r meighting): y = 1 .07 x+-0 .00685 (r=0 .9996) 7.4 TO - 6 .5 - 6 .0 5.5 - 5.0- ' 4 .5- < y 4 .0- i i 3.5 . J / 3.0 m 2 .5 2 .0 1 .5 - 1 .0 . 0 .5 j / f - 0 .0 0 .5 1 .0 1 .5 20 2 .5 3 .0 3.5 4 .0 4.5 5.0 5 .5 Analyle Conc . / IS Conc . Figure 28. Representative calibration curve for 8-2 FTUA by LC/MS/MS . r 6 .0 6 .5 p. 75 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -74- Project No. : 112E-108 M 042006 M12 TELALAE.rdb (PFOA C-8): "Linear" Regression ("1 h,"weighting) : y=1 .12 x+0 .0538 (r= 0.9985) 7.5 7.0 6 .5 6 .0- 5.5 - 5.0 - m < - 4 .5- m 4 .0 m m 3 .5 ,./' 3.0 m m 2.5 2.01 .5 - ,/ ., ' 1 /J 1 .0 - 0 .5 0.0 1!~~ 0:5 1 .0 1 .5 2 .0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 5 .5 6.0 6.5 AnelyOe Conc . / IS Conc . Figure 29. Representative calibration curve for PFOA by LC/MS/MS . p. 76 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -75 - Project No. : 112E-108 042006 M12 TELALAE.rdb (C-9 Add): "Linear" Regression ("1 ! x" weightFi9) : y= 1 .34 x + -0 .00636 (r = 0 .9991) 9 .0 - 8 .5 . /r 8.0 j r 7 .5 7 .0 - 6 .5 - 6~0 - m < 5.5 - m 5.0 ! ! / / m 4.5 / 4.0 3 .5- m 3 .0 - 25 . i f 2.0 ~i 1 .5 - 1 .0 - 0. 5 0 .0 ~ 0.5 1 .0 1 .5 20 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 55 6.0 6.5 Analyle Conc . / IS Conc . Figure 30. Representative calibration curve for PFNA by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -76- Project No.: 112E-108 ~ 042006 M12 TELALAE.1db (G10 Acid) : ' Linear' Regressi.~n ("1 /X' vneighting): y= 1 .4 x+-0 .0404 (r= 0.9996) 9 .5 9.0 8 .5 8 .0 ! ~ 7 .5 7.0 y 6.5 d 6.0 < m 5 .5 D 5 .0 r /-~ / ~ .r ,rj/ 4.5 v i 4.0 3.5 m 3.0 ~ i ~i 2 .5 j 2 .0 1 .5 1 .0 r 0.5 ~r 0 .0 0 .5 1 .0 1b 2 .0 2 .5 3 .0 3.5 4 .0 4.5 5.0 5 .5 6.0 6.5 Analyie Conc. / IS Conc. Figure 31 . Representative calibration curve for PFDA by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -77- Project No.: 112E-108 042006 M12 TELALAE.rdb (C-11 Acid): "Linea' Regression ("1 I~f' weighting) : y= 1 .29 x+-0 .0743 (r=0 .9994) 8 .9 . 8 .5 ./ 8 .0 . 7 .5 / i 7.0 6.5 y 6.0 - m 5 .5- < m 5 .0 - D 4 .5- m 4 .0 j / % / l D 3. 5 - ,/ 3.0 - 2.5 2 .0- l/ ' 1 .5 - 1 .0 ~r " 0 .5 0.0 f 0.5 1 .0 1 .5 20 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 5 .5 6.0 6.5 Analy1e Conc. ! IS Conc . Figure 32. Representative calibration curve for PFUA by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -78- Wildlife International, Ltd. GC-MS#2 MSD Serial No . File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : C :\HPCHEM\1\DATA\ALFI005\0701011 .D fjl 30 Apr 105 GCMS#2 4 :10 pm 1570-TEX 7 3118A02483 Abundance Ion 31 .00 (30 .70 to 31 .70) : 0701011 .D 3000 2000 1000 ime-->4 .00 4 .20 4 .40 4 .60 4 .80 5 .00 5 20 5 40 5 . 60 5 . 80 unaance, Ion 95 .00 (94 .70 to 95 .70) : 0701011 .D 1000- 500 Time-->4 .00 4 .20 4 .40 4 .~60 4 . ~80 5 .~00 5 .~20 5 .~40 ~5 .'60 5 . 10 Figure 33 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Ci8 cartridge extract) from a Day 0 untreated control soil by GC/MS . 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -79- p. 80 Project No.: 112E-108 -., Figure 34. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS. 8-2 FTOH was not present (no peak at 4.18 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -80- Project No. : 112E-108 Figure 35. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS. 7-2 sFTOH was not present (no peak at 11 .9 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -81 - . iw.-V .jmwoFm.-- ~~ . 1~-J, T~ : 14 81-k W "os 30 S.p . W,d51p . H.ighl : ; .T 1*.k V. W. MMOO p. 82 ProjectNo .: 112E-108 25MOD : 2MM 1~w i mo. o W I Printing Date : Friday, April 22, 2005 e.a ; 70.?" 7 :7.V 0 .,310 .M_ P. - .1 uD7_s.az>qv.r __ _ - 1 2 ] -" 6 E 7~ E 9 1D ~ 111 ~ 12 - 13 715i 16 77 1! ~fY Twr .~ . .. . __ . .- ._- ._ . _ Figure 36. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. 8-2 FTA was not present (no peak at 12.2 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -82- p. 83 Project No.: 112E-108 I ~.. i ' i ' b ' n ' h "b t O J Figure 37. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 untreated control soil by LC/MS/MS . 7-3 Acid was not present (no peak at 11 .6 min). p. 84 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No .: 112E-108 -83- ij =~nqla , wk Nan,a. "ea tur uaa.ar ~+s/ .mss 0 .Na~ il S co-nm.r. " ~-C). Am+ken- 1 . IM.[ ], 11 Saaple i~ : Concmi[a[im: .1..k 0.0D uq ..i ./L 7 00.3 i ~ G1culdeE Canc : N/L 'Fc9 . Da't~e : (I M4" D,/Il/Ds D7 :10: D, PN q soa, ; ~ XoeiH~C: Mvwl,i,q t.cto[ : ~ Novc TY,[eenol-: ~~ Aiu t6[.aM1e: n x~ . . s.oo . vineawtna 6x0.cte0 AT: s .P . xietn : li s .P . N.1qet: sxp . De.k Rano : '-p Ae~A.tan : j:i: RzP. vel. R ; Ufe Ael+nvet ".T : 1 201 .93 ]009 .6, ao .D 12 .3 0 .11 D .Dl 5 .00 , .00 3' .00 No cPa =va mn I B OON ~ asw+ e.ow i T7.01~ I 7 OON ~ TyW: iI RetsntiM SSa2 : Ate.: I~Ne ;qAt : i~ Su[t Ti.e : ~i ~a:E Tvu: Na Nn N/A N/> N/A N/A N/A cnvn s cpant sfn 6EMa~ 600~" ~ 6.SOa1 i S.OOa, : 100a . ~ 00_ uuE 1-41 ].SDI 7.OON 7.fi0a1{ 200a, I 1 , .OON 1 7 .OA, , lODO.W ~ . .. . --- -- "-- ------- . .. . Printing Date : Friday, April 22, 21M15 ea e.~, Ta w 10.15 11 .10 ,zrr, ,1a .,ass _.. ~ s er --'e ~~ - o +o t+ u +a u i's sn ie +a- I TwN. r~n J Figure 38. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . 8-2 FTUA was not present (no peak at 12.3 min). p. 85 Company Sanitized . Does not contain TSCA CBt . Wildlife International, Ltd. Project No.: 112E-108 .""~'n s. .p.N.m.:- :pN=var s.mpri~T:roar~ob=Teuue..w;r Wax.m.:YFWCr w+luz'" t3.~lW .o .mt j ewMrne'n]E-tro.t00.tt0-(t1TCr Amebten :- ~I sa~l! InGU: : I. :i sa~l . Tro.: EllnA , c~.~nlnciauen : , caleul.cea eonc c .oo p/a uv a.i ./>. " Acq . nace : 0!/23/O S t.oas ~ Acq. Tfme : 01 :t0:01 1pN ~poEi_ilQ: No ~ ; mmc0inq F.ctcr: 1 I ft- ThmaOOIU : 189 .77 cpa Nea 11:reaTOlA : 941 .84 epa ' pu. . SwoCha : I ; ; AT Ninaow : 3o .o ; . E.wccla A- : s.s : an i 5!D- Niam: 0 .20o 5ep. N.iqht : 0 .01 II ErzD- -k s.oo Ezp. AC7 . R.cvo : I.Oo i Exp. Val . Ratvo: 3.00 Va! RellfiV< RT: 4o s.ao.! i ?.so., ' : LOV Type : I Aa .nclon II Arla: YCaq[c7 Tiw. i.e : il EiW T1Ne : po PuR N/A N/A N/71 p/A N/A cow, , cDe C nin sin E,~ .iIn< . ; i B.OMI ) ~ S.bO.i I ~ xD.'j L ~3Wl,1J tAM! 3 .SON 1 3.ml1 tAM { 200N'I -84- 1 II 3700 .W i 1~ t~ 7 " .S 6 7 D Y 70 11 12 71 1a 15 18 17 1E Figure 39. Representative ion chromatogram for PFOA in the soil extract from a Day 0 untreated control soil by LC/MS/MS . PFOA was not present (no peak at 9.82 min) . p. 86 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -85 - yy~-~~^^~_ mpYN .nw .'Snnpl._007 BrrW+1 .'l5 V "r:'Oa~00_TEIaIAE.wR / Pwk Wm+:'Gi .cIC kYWwl:'~t.W1YD+inf !I . c~,rrc "112E-10l,10P,110~t7TCr ~kon.- ii 9Yple I1W.r : saeple T: ype .e<rataon: La s1mk 0.00 ug +.v ./L I.ODrS , ' Ca3wlated Cw~c : N/J~ 1 ~~9. O+Le : ~ Aca. 7iae .. Oa/xl/DS 07a0 :D1 PM 9 SO+a + r.odiLV.E : eunUing Faccor : I Noiae f: t-hold I ue+ mteenole : .. r, . s.oew : X7 Y1oGd: Eip"cted XT: Sep. NSCth: ~~ S.DNeL9hc : LxPxW] " k+cvo : Exp. Val. P.LVO : Vae RelaLive 11T : No 1 192.C5 964 .2s t 30 .0 ]0 .6 0 .]0 0 .01 s . DD a.00 L00 Xo cpa nP' man tvm7vne . ku : Me1gNr: ~I SurL 7va.: EeW 7v~e : No X+t N/> N/7. X/A N/A > cpa c ~1n vin P OI4a ! esD .a jI eDDea 1r 750+" 7 00./ ~ e7Pa i 6.OD.1 " : E.SMa' OON "OO.a 160." 3W.a ].bDe1 7.00 .! 13D+a 1.OOr" 9000.00 000 Printing Date: Friday, April 22, 2005 202 7 .]! I,b 5. . &OI l.] "_ 7 .! 0.f0 107l-tO.SB11 JaJ2~T.."1r1 2301J.,TT_ . __ j-3 1 ~ " 6-7 ! 9 10 17 1] 75 1 " 15 ^76 17 r 1 .1 - Y - Figure 40. Representative ion chromatogram for PFNA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. _ PFNA was not present (no peak at 10.6 min). p. 87 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -86- Project No.: 112E-108 SanpYwm.-J.npN~WS 9amp. .-+~0'-kaY.q2f05_00_TEUIAE..[T ~~ Pcwmt..w.~.c-.+'fY2-Et-O,Anet.i,Cou,Anlowlyuerlc'refAJw.WaNmlE.OO O.-mf -" ~ l+.Pl. vaa.: 1 4 l.Npl. 7ype : Blank Cencencr.cvan : O.EO, uq ..a ./L + 00.! I Glculaua Ew.c: N/A Ace9Ora u: : 0!/21/OS mao:o . an Pao.. Noaili .a : .7WnrAtnq F. .i Nea< rnt. ..naa : il ~u 9nreabola : XiMw: ;I Gvpecyea A2 : '~ s.p . aacn : ' EYp. Mev`n~ 0. f"aa tlo : e.P . vu.il . . x.ua: n. ic : u. :A.latavetxr : Nc 1 ls: .lo lie .50 1 30 .0 71 .2 0.20 C.Ol 5.00 . .on = .oo uo m= R. ~n e.ww a .mw~ > .lo.. I >.m7s ~ " ~~.nvionraw: Araa : xia N/w e.wtn e.owa a.ew 9 -4 1 4.m.a J ffiW ~ 3.OMi~ 23M'1 -41 i3SaI t .COaa SOOD.W oro T, + IPrinting Date: Friday, April 22, 2005 -- x~_a . !.,! .Jn.Me.ae a.o+ AE rn.>s> a~nT_>oy tf.t+.o ,l.ao.f_Jee 2 s ~ s e > e s fo n n ~ ia u __ 1$-e ti __ +e Figure 41 . Representative ion chromatogram for PFDA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. PFDA was not present (no peak at 11 .2 min) . p. 88 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -87- '. ~ y:,p,w::,. "ar,p.-me" aw,yrn. ",570" fr arnos_oD_Tuuewr___ 1,, PrtWUr:Y-11 .1sf Yrywl~ "3e7WS,p.O .mf D.,w..~c " n2e-,m.,m. WUTC7" .:.w .en- !i s..pl. xna..a peyle Tyye : blank +~e D .pO up .a ./ : cacxi.c .ao eca . wY: c xn, aaxr :vas xcy . r , r-lo :o~ m pso..:, ea.a: i e~wex::w nccec: .nola: llc .D3 ~' kwrna ..n.la : s7D-ls ccyp . xa~awTx~ ~xD .o c.a a ll .s .a :, 3p.iYSaNTC .20 Xp . xeapxc eap. Peat R.nle : s-OD ; Ly . Ta7 . R .00 ~i pp . va . rt 2 .00 e Nelalveix.. se pmw; esD .~ 1 enw ; 7a1. >DOa: II ~i ~ mT on Ta .e . j ~a T~.x : 7Naqxi : ~ Si.[i Ta.e : N,.x/At x/R N/A N/A e~ a .,nCpa c eeo..I 6.mw~ Project No. : 112E-108 o.. : >.DD.. : 2.7. 0. 2.mM; , .m..l 70pDm i om -- + Printing Date : Friday, April 22, 2005 ~ ~ ~ i- i s.a am as, 7~p de 7 e .. p~ a ,p Tw ns,. ++ar,tm ,a.an~ + n s +~ +s _ u+i7s -~ +6 -- - ----- -- - - - ------------ Figure 42. Representative ion chromatogram for PFUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. PFUA was not present (no peak at 11 .8 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -88- .. ~l~iaeil3fe I 146D 54ria-1 NO:; . 31:1:]: :0 nU:#33 ri, zg :. ~Op:e~:st-a;x ::: Y7~it-d Acqu~.red : $~Le 1S~,e : H14-0 3xi ffl: X:-..:-~-.qc"s~2--\ i AT~ Iq ! t' ~~fl-7 A n-i ~o.r~~oi .a ~ ~ 3: : p~ pitm. I~~:rT~s ~ 12.~--I f~8y 1 Q~ . ]:1 Q ~ -1~+t3~2i~e. ?~f1 o:{i I0ft 31 . 0r.{3 (34' 0 .7t 01 o LOOM : $a Tisiie,--s ' +4 , 4-0 4 :4 5 . 40. ~ 5 : 'tCJa: vvY Y 5 . aa '200. :5'~2'a. : Figure 43. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (CIg cartridge extract) from a Month 24 untreated control soil by GC/MS . 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -89- Project No._ 112E-108 Figure 44. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS . 8-2 FTOH was not present (no peak at 4.18 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -90- p. 91 Project No. : 112E-108 Figure 45. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS . 7-2 sFTOH was not present (no peak at 11 .9 min) . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -91- p. 92 Project No. : 112E-108 lo= . , ~ , -- _ . . .. .. .. ., . . Figure 46. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace 8-2 FTA at 12 .3 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -92- p. 93 Project No.: 112E-108 Figure 47. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 untreated control soil by LC/MS/MS . 7-3 Acid was not present (no peak at 11 .6 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -93 - p. 94 Project No. : 112E-108 1~..- _.. , Figure 48. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS . 8-2 FTUA was not present (no peak at 12 .3 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -94- p. 95 Project No .: 112E-108 Figure 49. Representative ion chromatogram for PFOA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace PFOA at 9.91 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -95- p. 96 Project No. : 112E-108 Figure 50. Representative ion chromatogram for PFNA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace PFNA at 10.7 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 96 - p. 97 Project No.: 112E-108 Figure 51. Representative ion chromatogram for PFDA in the soil extract from a Month 24 untreated control soil by LC/MS/MS . Trace PFDA at 11 .4 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife InteYnational, Ltd. -97- Project No.: 112E-108 I~~.= ., .. ., - .. Figure 52 . Representative ion chromatogram for PFUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS . Trace PFUA at 12.0 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -98- Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : C :\HPCHEM\1\DATA\ALFI005\0801012 .D fjl 30 Apr 105 4 :29 pm GCMS#2 1719-TEX 8 Abundance Ion 31 .00 (30 .70 to 31 .70) : 0801012 .D Figure 53 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C1g cartridge extract) from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by GC/MS . - 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 99 - Project No.: 112E-108 Figure 54. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 fortified (250 gg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS . p. 101 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No .: 112E-108 SPmplaNPer''6arqla-OOT SPmpY1D:'+718- FY'YH21bg~DG?EUUEmIP P-kNemero-21sy wP.g.al:'m.m9a.o .pw I -R1c. ~~N ~.-'112e-tm .Itsw.no-~swNer Aleorw~ - j s .eDle TyDe: CM -"ti- a 30 .0 Dq , .ooP1 I jlGlculxnd cxs 7.7519 W a.i ./ke F IIAO9- Dat< : . OV2]/05 P~AO.ao i ; Acq hse: 07 :77:27 PM Moeili.d : I nunwny L I Neiae TnreatwlC " Acea TRCeaNold:~ I~ 4it. ]~oeNa : ~ RT NiMw : II ~; ExDeYed R* : sep. ldtn: i Lap. N.ipht: ,- Lsp. Pe.k Aatio: '~ LxD" AG7- 0.atio: I Lxp. val. Re ao : e Ds . Rel.civetRT : No 1 31 06 130 .11 1 10 . L 1 _ ._ o .20 O.ol SAD 1- Ne D cpa epa .u, II In.0 7ype : ~ :ent on 7iae : I Re19ht : II Ciact hw : LM Tiar : aese -.-.'2.* sln 15075 . ounb 2931 .21603e cpa 12 .0 ~n 12 .i 9oao 00 ~ &SOp W f L000 DU j 76 .0 1 700000 { S'OD.ODROOe.OD~ ~fiLL.00 ~ I .070.00 i 1,SOO.W I .WO-OUi 1SWDU , 900D-00 i 3lIp.00 ' 1000 .00 ~ I 110D.00~ 100D.0U i LOO.Wo -100- 1223 6.W s sT'-~ 10~ 11 .09 - i c +o~++iz 7Yw . ~ 7A151] .!9 iau ts _. ieiiteie Figure 55. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 fortified (250 gg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS . p. 102 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -101 - S.mPbN.m.:'Srrq4_ ~.4~ID:'777C FW :'04f05~GU_TEIMAE .riIF F'pkN.mr32(UY kbWn1~457,098].O .mf Comm.rk'172E-tOR,tOG,170~SPINEY M .qYbn:- Suyls InMx : 75 SuWls xype : I Ceneanctac3on : OC 10 .0 uq - ./L 1.0.061 I Caiculated C: one :~ Acq_ Date : ;Acq . Ti- 8 .3876 uq s .i ./kq 04/21/OS 07 :31 :27 p!f ' r 8~504'I Nodntpad : No Dunchnq Facioi : 1 . Noiee Shreaho1d : 201 .93 cpe Anea IITneeholL: 1009 .65 cpa Nm. amooche : 1 , RT VcMw : 30 .0 Lxpa LeC Rx : 12 .3 ain I ; Sep . Sep . ILxP . I dP . ' ~ CxP . Vndth : Neiqht : Peak R.cio : Mj . Raoio : V.1 . Ra>o : 0 .20 0 .01 5 .00 4 .00 IN." ~lnc . xyPe : ~~ Mcancion 7ise : F Araa : 34iqht : sc .ac ?iae . End x1~ee : e.aa xo e. .. 12 .3 140610 . ounce 26908, ;6250? epa 12 .1 i n 12 .5 P.~4II 8'S.04{ S_00.j 7.50.4j 7.00.4 1 6.OON i = S.SON Ii RP 500.4 1 ' 4 .50.4 - 4.OON -1I ] .SOM i 1! 100N 2~ 2D0." iI tlb/ 1 t..l i! 6o~.m i~, aao 123 + 5` 72.2D 8--7 3-8 e .2_7 s.e . to.Is 77 .73 172.n ta.a 7 e a i0 ti i2 t3m1516"--'8'; --- Figure 56. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 ug PFCAs/kg) control soil by LC/MS/1vIS. p. 103 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 f! ympyNRm. :'SLmq~ 00 mqb10:'1719' FW:'0171~~7E1AU~~f VrkW;n :'DFOACd IM.ywY'~19019BB.O .m;f I u{svRCEr ry .wr+en." 3aRle IM.z: 15 SasDl. TyDe : iI Cnncentucion : 30c.=0 uq + . : ./L 1.00.5 . IIC+1cu3+teE Conc : 9 .1702 u9 a . : ./k9 I Mp . 04ce : 09/21/OS I~ Mp . Tawe . . 07 :31:27 DM j-i Ia f d : ~ "urK":n9 Lactot : Ne1se h- Id : '11CeRThvshold : ;' R,u. Smootb : ' XT Windwe I' ;:p.c~d RT : D. . .d 1 : I3eD. N.iyRt: I! !- Y .Rk Ratio: iI L~D. m) . R [xD~ v+l. ... ;1 Oae RelatSVeR7 . Ro 1 189 .77 9i8 .e1 1 30 .0 9-82 0 .20 0.01 5.00 .-00 3 .00 xo ~Da cpa rn 9~, 850. . p .OpK ; ' T50.4- T OON " 1 lnt. Sype : il Ret.ntion Ti,-: .I x,a9"t : Scut Ta~e : 6~,d 71r: 9.91 170090 . min 28277 .51469 WtDa 9.67 10 .0 ain I 8fb~" I L.OOw, i S.SON I E - 4~ 4 .OOM 3 .SOr11 I 9.OON ~ 230N i i 20M11 190." I I fA0.1i sooo.ao! o.ao I- -102- i 9 6 7 L 9 1'0- 11 12-13 1 " _15 778 7--18 ` tY _ T..R.-. ~~~... .... ...-. Figure 57. Representative ion chromatogram for PFOA in the soil extract from a Day 0 fortified (250 ~tg 8-2 FTOH/kg and 10.0 ~ig PFCAs/kg) control soil by LC/MS/MS . p. 104 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -103- ~j sarnpYqama's.npY_ smpw3o:Y219' PY 131],~~TEUUEwT PwtNar :'CiMd Y.yaak'a9a.a~:6t +"A.aai ~i Cervrt',72E-,09.+09.7+OjaP9fEr - II s+aple LNU: 15 i s~h =YPae OC 10 .0 u0 a.i ./L t lculataC Cenc: 9 .7699 uq a.i ./kq I Acq. dw: D1/27/Ob ka . rir: D'r :31 :z, w 1.OG9 s~D.+= MahliW : : e,mcMhq Fa[[Ot : aho30: .'AreaalTraaM1Q : j M y a.eetln : 100w: II Cxp.c W A:: t NaGth: CPl+ai9ht : p. PoaR 9 2 0: I~~Pk~J " 9at1o: ~I Gxy. v11. tatlM1 : : Vae telat,ve . Fo 1 393.95 96, .25 1 30 . D 30 .6 0.2< 0.07 b .OC I .00 3 .00 e rya cpa .tn B.Wa61 "b0.a ; appH{ >.a0a1 I ZODN' C ~: ivr arw~ Nsta1K : tt Ta7: ero r.a : t t Te aa10 .6 ~ 226360 . - fe665 .99635ewcpe 30 .6 lo .e an ' "~a : 59M a.ODN - " 9pM i "OW ~i a.a0.t zmr tsa6~ zmw { ,~ I{ ,aw i aaDm i ami 2 i-_ _. tnrn ".g_w.,a,ey~+Dr . >,r s i r.,94, nis Figure 58. Representative ion chromatogram for PFNA in the soil extract from a Day 0 fortified (250 ug 8-2 FTOH/kg and 10.0 jig PFCAs/kg) control soil by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. I~ e.'n`N.n,. " snm`_oor s.~rorio. "trlr rs . .w :cqcotewwe: wvNr . . . "caowc sry..t's,aaws.o ...r i ca .n .m "nze.,ae.lw.ttoiav,KEr .imossw' s.Rl. lne..: ls ~ suolc r ~ cnnc.ncuuen : lon uv . a./n c.oo.5 ; c.loa .c<a cone: s.'ae ~q .. :./aa I; r,cy . rir: o~ :zl :z> nc mlno r I Nolae LTCeaVelG: .. ThrarNClc: ~~ Ihm. S.eorm: Nr rlmw : I as..nc.. c~lbacn":: : il ~ n. xaqnr: .v. c+rv. ...v ul . cace: RN.cio : II e. va . : ~ Vae NelaiveCRT : t 767J0 B3e .50 7 so .o zo l..zzo o .ol s .oo , .oo 3- Ao .. : P.un'CMTi l.re Te ape rye vn . e..yj. ' s.ow1~ r .so ..a -1 .in ~ff Sos, ~ - " COa I 7GW. - 104 - p. 105 Project No.: 112E-108 Figure 59 . Representative ion chromatogram for PFDA in the soil extract from a Day 0 fortified (250 gg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. p. 106 Project No. : 112E-108 -105- I!~~~-~ Dr"-r~ yYwnr: "Eimpf,_ Dr~YI . , ,r Fil..'GZt~[70=EIAtA .I,r . M4Nrw'C- 0.uC W.ywY~DD.0D'3,DA.m! P o...,.~c- 2e-,oa,aD. otDDRiEr ti.....e:- s ..ol. 2ca.x: 7s ~~ s.yle ryp.: pc 4 c.io+t.:ed cmr: s.les~ ~D ..a-icD ;:4cR . o.ae : winios IIF,c . t- 0~ :7, :n > D.saH : Radtfi.d : ( Ewe :a?D Faccov : nr .a :wld : !i~.. rnr..neia ' :nm. s.eocnr : 0.t Y :ndw : ExpecHd RT : Sep. vlael :: sap. Wi9nr Dzp. Mat R ' EnD~ Ad) . 0.+cio: ~ Lxp . .l . R.cao : , . R.Lcave RT : le 3I . .U3 570I .15 30 .0 1] .D 0.20 0.07 5.00 ..00 3 .00 Ne - m. .in . esow D.DD.j ISDaL- I f Int :~: Dnc : r,wc : c,w T- !se To Gw 17 .E 7znEll..DsZZSS~cv" Es"II E .m.. s ao.. i F I t70H I ..W .~ ~ ]70a1 aao.c= 2 sw "' 2m..i .n , m..; soopoo= oDO---T'~-'- 2 2 " s .m s.a, aaa 2.DUa i.n sr. ./ !52zqys, .,aeE_ S e r -e i 10 ' 1, ,--~17 14 is`.. 1. 7~tn . _ _I '? ,e .--19 I ....~ Figure 60. Representative ion chromatogram for PFUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 p.g PFCAs/kg) control soil by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 106 - p. 107 Project No.: 112E-108 Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : T :\DATA\ALFI025\0801012 .D DW 20 Apr 106 8 :04 pm GCMS#1 1735-TEX 112E-108,109,110 8 Abundance Ion 31 .00 (30 .70 to 31 .70) : 0801012 .1) 2000 - 1000- ._r,_- .--1 -~,T-1 ----TT-r- ...,-T-T -r-~ ~ Time-->4 .F0-0 4 .20 4 .40 4 .60 4 .SOT 5 .00~ 5 .20 5 .40 Abundance Ion 95 .00 (94 .70 to 95 .70) : 0801012 .1) 250 - 5 .60 Figure 61 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (CI8 cartridge extract) from a Month 12 fortified (250 ~tg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by GUMS. 8-2 FTOH was not present (no peak at 5 .0 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 107 - p. 108 Project No. : 112E-108 Figure 62. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 fortified (250 ~tg 8-2 FTOH/kg and 10.0 ug PFCAs/kg) control soil by LC/MS/MS. Trace 8-2 FTOH at 3.98 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -108- Project No.: 112E-108 3 Figure 63. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 fortified (250 gg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 109- p. 110 Project No. : 112E-108 - - _ Figure 64. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 fortified (250 gg 8-2 FTOH/kg and 10.0 jig PFCAs/kg) control soil by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -110- Project No. : 112E-108 .,a,. - :~. ,.,..,; .. . ..,~ ---- ---I Figure 65. Representative ion chromatogram for PFOA in the soil extract from a Month 12 fortified (250 ~tg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 111 - p. 112 Project No . : 112E-108 Figure 66. Representative ion chromatogram for PFNA in the soil extract from a Month 12 fortified (250 jig 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -112- Project No. : 112E-108 c.onm'a,,wrwrxr~tn~.w.m~.o.,..,N J~,anns..r .i Figure 67. . Representative ion chromatograrn for PFDA in the soil extract from a Month 12 fortified (250 ~tg 8-2 FTOH/kg and 10.0 ug PFCAs/kg) control soil by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 113 - Project No.: 112E-108 ~.:~. ,..pv. . ..,.,. ~~ Figure 68 . - Representative ion chromatogram for PFUA in the soil extract from a Month 12 fortified (250 Rg 8-2 FTOH/kg and 10.0 gg PFCAs/kg) control soil by LC/MS/MS. p. 115 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 - 114 - Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : C :\HPCHEM\1\DATA\ALFI005\1201020 .D fjl 30 Apr 105 7 :04 pm GCMS#2 1665-TEX 12 undance i Ion 31 .00 (30 .70 to 31 .70) : 1201020 .1) I 2000 1000 1 l ime-->4 .0.0 . i Abundance 1500 ~ . 4. ..2.0 . . 4. ..4.0 . . 4. ..6.0 Ion 95 .00 ' VV- T~ ` 4 .8 0 5 .0 0 5 .20 (94 .70 to 95 .70) TI "~ 5 .40 5 .60 : 1201020 .D 5 .80 1000 - 500 - Time-->4 .~00 4 .~20 4 .~40 4 .60 4 .80 5 .00 5 .20 5 .40 5 .60 5 .80 Figure 69. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Clg cartridge extract) from a Day 0 sterile soil treated with 200 mg/kgC )by GC/MS . 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -115- p. 116 Project No.: 112E-108 Figure 70. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 sterile soil treated with 200 mg/kgE )by LC/MS/MS . Trace 8-2 FTOH at 4.09 min was <LOD. p. 117 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 -116- B++piliaiii+ . "'8ir+pa_Dil~ '6~~YIMDS''wxtM05kL_DO_TEIAUE. v+atNN,w : "9-xlar Na+y+N'~n.mqo+mr na,anc ",txE-,ps" wNeasn.- s-l .l:w .: x0 swple ryp+ : untne~m N/A 1 .m+r G]cul+vM Acp. D p. Taw. Cnvc 0.0000 u9 p~/27/09 09 :38 :71 RI +.L/ty iXD.m IbDan,W: wc rq T+nLpz : a< TLrmlwld : e+ TLVa+we16: ~YiiWe+: S+pecsM FL N+aqt.c : G0h+t R+cao: Lxp. Ad7. P+tte: Y+3. P+ + R+l+civaAT . ~I Int. Typ+ : I u - en icw. ~ s+spnx: ~ un n .+ . No 7 3".OE :20 .71 )0 .0 0.07 S.OG t.00 ;.00 ut niA cv' c Nan + ccy~s T .tn . .n 90m.m 7 sspa, il{m l.0VAOI 79WA7 ~ >lmA7 ~ ' S7m.m' ~V wpo.m : S > -um.m : ~WOmj YmLD J 700nm I 3mL0 { 70pOD0 ~ ,9mm I tWO.m~ om Ptitding Date : FEiday, Apri122, 2005-_ 0 ." i- i e s 's r' s 9 to 7 "n-v+' luis ,i n ,. Figure 71 . Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg by LC/MS/MS . 8-2 FTA was not present (no peak at 12.2 min). Company Sanitized . Does not contain TSCA CBI . Wildlife InteYnational, Ltd. p. 118 Project No.: 112E-108 - 117- 114 sarrpN.rr. :-s .rro~a-o1r s .Np .1o-t Pr 1 _ _ prY wm.~ "e-2 (ur wW ..) '~57 oRg3.0 .nw ' Garrww .tY12E.f06" Nwwoson- S .ople lm .a : 20 Sasp1 . rypa : -k- Cwrcentratlon: N/A 1 .OOa5- Calc1v.nW Conc : ~1cq . Wta : ; acq. Ti- 0 .0000 uq a .a ./kq DA/31/OS 09 :1P :11 PM 85Ma i ;; Modlfied : No ; nunenlnq racter : 1 ; Nolae 7nresnola: 201 .93 cps ~: Area rnteatwld : 1009 .67 1, Moa. Snootne: 1 II RT Ninaw: ]0 .0 !%Peet.d RT : ' sep, Naatn: S .p . Raiqnc: 12 .1 o.zo 0.01 Cap. Peak lutio: 5.f0 Sxp. JWj. Rateo: 4.00 ~i Cap. Val. Ratio: ).00 r~ llae R.lativ. RT : Nc 900aa a.eo.a e.oaal T.5.0" ; 1.0.0~i Ret .ntrqnTrse : ku : : M.r9nt: il Start Ti! Cnd Ti-: Ne N/Ak N/A N/A N/A N/A cpa ta ~n a.5ow ~ 6.OOH x S.SON ~ .~6 S.OOa = ! F 4.SON - ..ODa, j i i50a. ; ...N' 25~.. J,, ZOOM j 1 .SOa, I; i 5000.00 ; Printing Date: Friday, April 22, 2005 OM", 7.M e.u _-6 ~~-) _~~ ! o.n te.t2 tt.t6 rtx oas_ 1ax1 9~-_~ t0 71 13 r.n.~ _ __ 11 5 8 17 _ 18 I' Figure 72. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg by LC/MS/MS . 8-2 FTUA was not present (no peak at 12.3 min). p. 119 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 - 118- 6.n. p H~,t" bwR`ID.',eOS }i.rot7i06~7F.UlAErT p~lxnn.: "PFWGd M.y.tt'.+J.U7q .0.mY Dwm.ntY,3E-,Ob' Nw`Ren' 1=]I: in a0 aS ea : Centmtcatien : t~.~l~cu~ enc: Unknovn X/.3 Ot/27/OS e.t ./Rq ~ , .OO.a'+ uq . x>~e . 09:]e:31 771 MaavlvM: Nng q - . Tett.nole : lesn7 ap, ua rnn[nola : qte .es cpt ~I~ x~.. Rr s~ .e.e:tm : 1 I 11 e.wt,r>,t.a Ar: s rietn : 3s .ez .>n c.zo v. x.>9nt: ccv eap. Nd R 5.00 9 .OPt e.mw ,e .m., 1 . R. : : . UfeRt1[qv[tAi: 3.00 He ~j xnt~Typ. : a>. "t I~ 9UrciTrr : eM re eas. Te a... .el luos . .A5193c~ 1 "79 .70 30 .0 ~an T .OOM 1 asw' 0.00N i I 6'At ~ {, amr ~ ..asw I awr~ aoow z~.. j z- ~ , DA01.- ~ -- x -._s" . - . . --~s-_ e7 e ---- " ,, 9 T-,0.- ,2 -,3 ~ - ~ - ---- .- - Figure 73. Representative ion chromatogram for PFOA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg (_ 1by LC/MS/MS. Trace PFOA at 9.81 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -119- p. 120 Proj ect No.: 112E-108 Lan,rR- 7E.706~ AmobLe, - ;I s+yl . 1na.R: :o ' s+.Pt . rra: vuinwn :, concmcucaen : n/A aloulaua coeo: o.oooJ oy +. :./kq ! Mq . 0 D4/31/05 M9Tiw: J9 :1!:31 M Nae>[>ee : Ne n~R,cn>np r.ator: 1 .. rnremola: lv :.LS cpa e. mr.+nele : 964.25 cPa N1>e. s.oen., : 1 Rr V>Mw : c.P.ec.a R 30 .0 lD .s ..n seP. v>acnoo. :o ,;s.P. N.;qnc : o.ol li LxP. N.k Wtro : S.CO ~ LvP. 1~7) . R+ >e : 1 .00 ILx~. 1 . R+ >e : ~.00 W.- .1 ; , oos , 9' B L.yos e.oo 7L.01 I --=!T- N/Ak AruN/A ~M.Sgnt: N/A Istarr T>~e: N/A M T> .r : N/A an cPre ~an a Dos : ssow~ sm.+! i IDOM ; .m..; 3.60 .1 . 900.. . 7'JON 3.00.1 ~ 730.1 ~ 1DO.~ EOOOWO.W Printing Date : Friday, April 22, 2005 , .~ . . 6 ._ ~`W __~_ . . . 1D9-0'~ IM ,&.0f^-10 .72 _ i o ~ ~ to ~ 11 ti 1a u-.is .. (L . ._ 3-7 _ n . mw . . . - ---J Figure 74 . Representative ion chromatogram for PFNA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg( Jby LC/MS/MS. PFNA was not present (no peak at 10 .6 min) . p. 121 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -120- s+.a+x .n .~ PetNwn :Y-tOAdC 11~In1771U'[89.O+mu' 7uql+ tM++ : ~ ~+R~+ +YP+ : II Calculataa II Ac9. D+:a : IIACq . Tict : t+rc: 29 OnFN/A 0.77791 0[/S1/OS 09:1Ba1 u9 + M :-/kq 1 .OOa5 I 8 I I d:n~eA1W : cte[: x 1 Folae TApehola : i Ars+ T1T+ohola: 167 .70 l36 .30 cp+ ~I~ N S.aotha : EcPaicMYewv:x: : ~~ sap. lace: s+p . xa.9nc : I EaP" t'++l Paclo: 1 7` .l 0.z ~t+ o.zo o .ql l.00 E+PAai- 9atio: 1.00 ExP. val. l+tta: 3 .00 1, q+e M .-l IA AT : xc iin.eeuon~T[.+: nAna: ;i IN19h[ : il 7tvt Ti : ; Cp Tl~+ : a++ihz~.in 7192 .7 ca 1226 .09099c..cp+ 31 ., 17 .3 wvn 9 I l.SO+,t I a.aow ~ 7.70+1 ; I 7~+11 esq++~ q S l.SOal i '! S.OW I af0+[ 'I ' I +00+[ : !'-0N AOON L 250N ! 200ac' t .6oM II 1 .OOa ~ 5000A0 S1 000 -- a 7 E 9 '--. to~ - tt t2t=~17~tl~~ t5 t 6 17 1 9 fY `~-- ._ --- ~---~ -- -.. .----~ ------- -- ~ -- -----------------~ - - ~ --- - - -- --r --.-~------- - - . . .-'-- - - Printing Date : Friday, April 22, 2005 Figure 75. Representative ion chromatogram for PFDA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg~ ))y LC/MS/MS . Trace PFDA at 11 .2 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -121- u;;w0aw. 5m~c _-_ -ND: '1'~ F"- :~,210-5U~Y~W,2 .0- M~.4ip- ---d C- 1.0-0 -i 1-1~ I U.0 " L~ : :,9k R`,4. : R- : X-- 0 .01 5 .00 4 .00 3 .0 . Ti-,ht : Ii. . T- .. . T . a57 l . - -: 12 .0 - p. 122 Project No.: 112E-108 ..mw ; )so .1i I 3~I z~ l 2 . .W ! , .x.1 ~~ {I aao, hinting Date : Friday,April 22, 2005 s.r~-amTm ~ a~a -+o.at,n.1a 11a1 12utJa_1T ai 5 a 7 a 1 10 11 1~ 13 11 1a ~~~te --~- 17 '~11 19 Figure 76. Representative ion chromatogram for PFUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg[ I by LC/MS/MS. Trace PFUA at 11 .8 min was <LOD. p. 123 Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. -122- Project No.: 112E-108 Wildlife International, Ltd . GC-MS#2 MSD Serial No . File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : T :\DATA\ALFI025\1301021 .D DW 20 Apr 106 11 :09 pm GCMS#1 1682-TEX 112E-108 13 3118A02483 Abundance Ion 31 .60 (30 .70 to 31 .70) : 1301021 .5 3000- 2000 ~ 1000 a , ~ime-->4 .00 I undance , ~ , , -,-,-, 4 .20 4 .40 4 .60 4 .80 Ion 95 .00 (94 .70 to 5 .00 95 .70) 5 .20 5 .40 : 1301021i D 300 4 5 .60 250- 200 4 151 1-- . ~ - T , ~~ . ~l U Time-->4 .00 4 .20 4 .40 4 .60 V 4 .80 "~'V -~ 5 .00 5 .20 5 .40 5 .60 Figure 77. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cl$ cartridge extract) from a Month 12 sterile soil treated with 200 mg/kgC _1by GC/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -123- p. 124 Project No. : 112E-108 Figure 78 . Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 sterile soil treated with 200 mg/kgf, I by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -124- Proj ect No. : 112E-108 Q -e T ' ` 3e i em Figure 79. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg~ 'by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife InteYnational, Ltd. -125- p. 126 Project No .: 112E-108 Figure 80. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg( )by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 126 - Project No. : 112E-108 Figure 81 . Representative ion chromatogram for PFOA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg(I ')by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -127- p. 128 Project No.: 112E-108 -T ,y..___ p- Figure 82. Representative ion chromatogram for PFNA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC ] by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -128- p. 129 Project No.: 112E-108 ` Figure 83. Representative ion chromatogram for PFDA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC .3by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -129- p. 130 Project No. : 112E-108 Figure 84. Representative ion chromatogram for PFUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg[ _) by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 - 130 - Wildlife International, Ltd . GC-MS#2 MSD Serial No . 3118A02483 File : Operator : Date Acquired : Instrument : Sample Name : Misc Info : Vial Number : C :\HPCHEM\1\DATA\ALFI005\1001014 .D fjl 30 Apr 105 5 :06 pm GCMS#2 1593-TEX 10 iAbundance Ion 31 .00 (30 .70 to 31 .70) : 1001014 .D Time-->4 .00 Abundance 1500- 4 .20 4 .40 4 .60 Ion 95 .00 4 .80 5 .00 5 .20 5 .40 5 .60 5 .80 (94 .70 to 95 .70) : 1001014 .D 10007, 500- ,Time-->4 .00 4 .20 4 .40 4 .60 4 .80 5 .00 5 .20 5 .40 5 .60 5 .80 Figure 85 . Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cl8 cartridge extract) from a Day O live soil treated with 200 mg/kg;L 3by GUMS. 8-2 FTOH is not present (no peak at 5 .0 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 131 - Project No. : 112E-108 Figure 86. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 live soil treated with 200 mg/kg(- ]by LC/MS/MS. Trace 8-2 FTOH at 3.93 min was <LOD. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -132- p. 133 Project No. : 112E-108 ...v Figure 87. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day O live soil treated with 200 mg/kgt ))y LC/MS/MS. Trace 7-2 sFTOH at 11 .9 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. C.m.pt -tt2E.,W ~-.. .o-o : "tse2' i . "a t v . .YN .m.:Y-2f5T 1Y.yl.)Y7,A900 .mf ' Supl. 1eM.x : 1, rtan~ ' I, [!)culaM ~. F[90! "FCp . T1aa : on~ <FU 0./21/OS OBa~a! M .ediffad : ': 8WK7ti7f9 " lp 's . T : A-Old 21 .06 ~< Ftu Tht..Mld: 120 .31 ~; Ma . 9epotl~a: 1 i RS aindw: ]0 .0 '~ Lapect .d NT : s.P . Width; li s! . w[ynt: 12 .2 0.20 o.m Eap. W!F Ra 5.00 y Lap. M) . L.p . vl1. w i! We X .- T : 1.00 3.00 w vpn cp[ s[n int. Type : -t... T- wfqAt: ~I St![t Tawe: ; e~e Tue: . To 8u! .612 .2 l73 .B1 cc~e IQ .093l3 12 .1 lz .f .~p 11500 .00 I BOOC 00 I L90000' YOOAOi Tsm.ao' 1 7WarolI l60D.00 ~ E000.00 _ 7batt.a0 ~ ~smom' - apoopJ 6mom I aem.oo I" wm.m I 2sw ao I 2aop.m' t9m.oo ,oepm~ soo .m~ Owl- i i -133- taT~ iiio n ,r., .a, t~ ~la ~ ti p. 134 Project No.: 112E-108 Figure 88. " Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 live soil treated with 200 mg/kg[ )by LC/MS/MS. Trace 8-2 FTA at 12 .2 min was <LOD. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 134 - Project No.: 112E-108 I ~.. Figure 89. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day O live soil treated with 200 mg/kgC- jby LC/MS/MS . 7-3 Acid is not present (no peak at 11 .6 min). Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -135- ''~s ."x~~prwxw : "a .mp._mr s.xww] : " 7 Camrx't73E-7C6' Iuw~. .n- i~ a..pl. IpMa : 17 ~I Supl. iypv : U wn Crnpntz.cim : n X/A I~c+lcvlac.a conc : o.oooY Nv ..i ./kv M9 . U. 01/31/US :I M07>w: Cl :11:1a Px MoaiSiM: wo ~Y.cninv hecoc: 1 i~we>. . Shz. .nola : zo1 .97 cpa .aru Tnreahela : 1009 .s1 cn . i xr. s~aocm : 1 wT w.,ww: 3o .o cxp.ccw xT z~ . : ~an s .p . riacn:o .zo Sap. xapnu sxp . na.k Mtic : I c .p . Aq . xaio : I Lzp . va . xauo : C.U1 5 .00 Loo 7 .00 Y.e Ml.tivs AT : xo Int Sype: xetmtim Tite : Aze": Mei9nt : aGtt TSX.: Lna Ti.e : wo h.h N/A N/A x/x N/A w/r ~in s cp. t an r~.:wx]as_ 7ElMAEwtP ~ Uos' w'~1 i ar,p,1 : saa .17~M ~ I , ~- 6.OW ~ ssoa #' s.ao.1 . E 'sa' j a.aw j z.ao.. ~ zaow i p. 136 Project No. : 112E-108 Figure 90. Representative ion chromato for 8-2 FTUA in the soil extract from a Day 0 live soil treated with 200 mg/kg~ ]by LC/MS/MS . 8-2 FTUA was not present (no peak at 12 .3 min) . p. 137 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -136- s .apain',sa3' w:wz+moD_ PwlN.ma''PFCAtd IA,yw1:'113 .D1il9Aaml LeimYM~bn:- I Suqle 1Max: 17 Saaple Typa : ,intnor., I C.ncantzation : N/A CalOUlated Conc : D.65385 u9 a.i ./iq ACq. Dau: DI/21/DS Acq. Time : 08 :11:18 PN tODaS~ ' nodiLeE: ~II lunchinq FaOmz: tbiae ThzaaholG : ea Thzuhold : Wot11a : RT Yi+Ww: . : Exyeet .0 RT : Sep. 57~dth : Sry. Mai9ht : I P:y. MaR Ralo: Cxp. d0). R.tn-: Cip. L Raqo : II D.e pelauve RT: No 1 189 .77 913 .l1 ] ]0 .0 9.92 0.20 O .DI 5.00 ..DO 7.00 Ro opa cpa un 'i lnc. Typa : " Receotion Tim: Atec Nea9ht : 9tatt T :Ye : P~M Tim: se .e Raee 9.T8O1 .1n t1317 . wnte 2960 .59297c cpa 9 .1 . min 30 .0 ,ein S.Um , 8.00" ]SDN ! Y~ i I 7.3Ga i ulw . 1 a.WN: 3.50.4 3.00e . V 2.00m ~ , ~. , .DD .a : 5000.00 '~ Goo ; J 1 2 i -i- 5 6 7 0 -0 +o +1 12 13 u s +e n +e ,a T.... ~. --- Figure 91. Representative ion chromatogram for PFOA in the soil extract from a Day O live soil treated with 200 mg/kgf _)by LC/MS/MS . Trace PFOA at 9.83 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -137- ~IC ~.M-2E.,W 9 .mp1 . Wm."S.npYUll' S .n~pblD.'1 ' Y. 1 Y.albm..'CiAOS IYWwI:'~E'7.OY~19.O .mu' I Supl . ln4x : 1' s ..pi . "rra : unanwn conc.ncracaon : x/ " ,oDdc .lcul .r .a cen< : e .nszs ~q +.i ./ .c ~ Mq . D.te: ~~I r.:q . n..c . 01 :21/OS Daa+ae px ~ xoalti .a : xo 9~ncn~nq e.ccor: 1 I Bol .< Tnr .enole : tq2 .95 cpa Arca Tnmnola : 961 .zs cpa xu.. s.ootn.: 1 Rr wmw: 30 .0 I cxpecua Rr : lo .s .io I~ s.p~ x,utn : o.x "I S .p . Ne: 0.01 ;i Exp. YnP.c1o : 5.00 9 Exp. AG7 . P.no: 1.00 -~ Exp, Val . P.r1o : 7.00 ' We Pelalve PT : No tnv. Typ.: R-1- TLre.: ~. Nelqnt : : Sc.rc Tar : ~ Ena Ta .e : E .. To e.ae " 30 .6 5511 .0 984.4 2 q cp.s 101 6.11 1D .7 .rn ' Em.. ~ I+o 75Ow 1 E~ ; , .wIC p. 138 Project No. : 112E-108 i 3 .9D..' 1 SDOwI . I zso.. ' z9aa= 1 .so ..- ,Do ..= s9m.ao - 7 - _2 - 7 ~ ~S - -t 7 E 9 10 11 7M. mn 12 ,3 14 15 ,E 17 ~ ,E ,9 Figure 92. Representative ion chromatomam for PFNA in the soil extract from a Day O live soil treated with 200 mg/kg C -_]by LC/MS/MS . Trace PFNA at 10.6 min was <LOD. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. ~....~- ~ .~. ~...,M~ _,.~....v ..,*. ,~ ... .. . -138- p. 139 Project No. : 112E-108 , , a~1 . ,- .---~ ; ~-tt-- Figure 93 . Representative ion chromatogram for PFDA in the soil extract from a Day O live soil treated with 200 mg/kgC jby LC/MS/MS. Trace PFDA at 11.2 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -139- i a.;nPlaHanr :-~anp7.Dn' 8.npl .ro.'780J' Pi .:'W2116_p0-TEIAUE.wT / P.ak wm.v ~c- 1 ~uc u+1.aC-m osla.o a..r - Comtinc'7tIE-t08-- M;ebBen - sa.v1 . xna.. : x7 S..PL. ira: unk.wn Cmttnezaclon : X/A laU.b' calculrt.d canc a .000U uq a.1 ./kq ~I M9- Oat< : 01/21/05 ; Aa4Ti- : 08 :11 :18 PM 8 ~ . Moaafa .d: ~ E~x~ raDtor : Noiae Tnzeanola : ~IAru 7reanoid : we:na : ~ kT vinew : Erzpectad RT : S.p . V:dth: Sep. Highc: Exp. P.ak Mtaa : ExD. Adj . Mtto : EaD" ~al . Ilatto : Uae PQatav. ItT : Yo 1 lts .o 570 .15 ] ]o.o 11 .0 0 .20 0 .01 5.00 s .U0 ] .00 Ilo cps cpa tun 9.OUaa B.SON i B.OOss ~ 7.BbI7.OON ;' Int. 7ype : '~ A.cancion ti~e : ~I K.a : . Yaqnt: c xa:. . : CxMrT :a:.: Yo ruk Y/A N/A /r Ya Y/T wmta cw nin O.SDM em.1 I t, SSON I ~S b.00H ~ ISOM ~ 10bs 3.SON + 1.ODN I 250.. A 2 7laow I .qOM ; 4--T- s T i 6 la77 p. 140 Project No. : 112E-108 i . 1'b le' ir is Is Figure 94. Representative ion chroniatogram for PFUA in the soil extract from a Day O live soil treated with 200 mg/kg( ]by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 140 - Proj ect No.: 112E-108 Wil.d3.ife' .lnt:eWnzit3.,orla1 .. X~t- . =^ "0.S#2 NSD: Serial No . ~118~Q~48:~ Ei-3..e :;: 3C : vC`iEMs1~;1ND1kTk\A1-M24-;1\-jo"~A1m: a: erator D-0 Date .Ae4ia13~-OiS : 38 Apt 1:G}-4 3 :28 Pm . I_ F. i. sVT,1. . .0ftertit .:: C04e* 1 ,~~pl .e :AI4,me s 1615-TEX Vi:a~ t~u~tbe~ : T T-c?A 31 .-0;0 75- ta M .. 70). 07-01,01-1 JD 25'0~ 1CfQ0 x 4 - : :g9 4 . ~~o. - . ~t....~anEe . .I"s~ .a ~ . ~o ~. 00 9 . : 0 34 :.7Q. to 9 :-< TO5? : t3"9Z3];C1,7:1._:D r~~Y) :2~io~ ISO, " -: . :. ... .. ` .V . " .. . Figure 95. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cl8 cartridge extract) from a Month 24 1ive soil treated with 200 mg/kgC _7by GUMS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -141- Project No. : 112E-108 Figure 96. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg[ ]by LC/MS/MS. Trace 8-2 FTOH at 3.96 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -142- Project No. : 112E-108 . . -n - n- i H u v v a o Figure 97 . Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg[ ]by LC/MS/MS . . Trace 7-2 sFTOH at 11 .9 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -143- - .;..r..k ~ p. 144 Project No. : 112E-108 . , a - sy Figure 98. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 live soil treated with 200 mg/kgC )by LC/MS/MS. Trace 8-2 FTA at 12.3 min was <LOQ . Company Sanitized . Does not contain TSCA CBI . Wildlife Intemational, Ltd. -144- Project No.: 112E-108 ~~~=,~~W~~ .~ Figure 99. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 live soil treated with 200 mg/kg t jby LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -145- p. 146 Project No. : 112E-108 1~ Figure 100 . Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 live soil treated with 200 mg/4 ] by LC/MS/MS. Trace 8-2 FTUA at 12.3 min was <LOQ. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -146- Project No. : 112E-108 71. ... . -- ~ .. ., . ,. .. ,. . ., ,. . Figure 101 . Representative ion chromatogram for PFOA in the soil extract from a Month 24 live soil, treated with 200 mg/kgC Jby LC/MS/MS. Note: Secondary dilution required and reported for PFOA. calibration range . Original analysis was over Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 147 - p. 148 Project No. : 112E-108 - _ ,... .,.. ~.. ,.,., .. . , ,., ="i - Figure 102 . Representative ion chromatoffam for PFNA in the soil extract from a Month 24 live soil treated with 200 mg/kg[ - -]by LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 148 - Project No. : 112E-108 :----~---,:- . ,. ,. .---~ --- --..- - . Figure 103 . Representative ion chromatogram for PFDA in the soil extract from a Month 24 live soil treated with 200 mg/kgC ])y LC/MS/MS. Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 149 - p. 150 Project No.: 112E-108 Figure 104. Representative ion chromatogram for PFUA in the soil extract from a Month 24 live soil treated with 200 mg/kg( 1by LC/MS/MS . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -150Appendix I Measured Concentration of Selected Analytes (~Lg/kg Soil Dry Weight) Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -151- p. 152 Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (pg/kg) in Soils Treated with C Soil Type Ulfisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOD =1IInrt of detection (0 .206 jig/kg) LOQ = limit of quantitation (2.0 gg/kg) Aerobic Live Sterile <LOQ <,OQ 17 .8 19 .1 32.0 36 .5 57 .8 61 .4 122 .1 150 .0 269.7 373.8 376A 441.1 487.0 558.6 367.7 344.1 340.1 362.6 410.7 328.7 365.9 329.1 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2 .0 <LOQ 2 .4 <LOQ 2 .2 Q.OQ 4.9 <LOQ 7.8 Q.OQ <LOQ <LOQ Anaerobic Live Sterlle <LOQ <LOQ 21 .7 22 .7 26.8 23 .9 51 .5 40 .3 70 .0 73 .3 114.7 133.5 148.6 104.7 <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ 2 .5 <LOQ 2 .8 <LOQ Thursday, Novanber 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -152- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (gg/kg) in Soils Treated withC Soil Type Mon;sol Sample Interval Day o Week 1 Week 2 Week 4 Mouth 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ a,flQ 15 .4 15 .1 17.8 18.0 21 .7 20.2 31 .8 30.3 46.0 58 .6 74.7 74.4 92.5 69.3 103 .8 94.0 98.5 247.9 126.9 118.8 229.3 207.5 <LOQ a.oQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ 2.9 <LOQ 3 .2 2.3 3 .4 3 .3 3 .0 3 .1 LOD = limit of detection (0.206 pgJkg) LOQ =limit of quantitation (2 .0 pglkg) Anaerobic Live Sterile <LOQ <LOQ 14 .7 15 .1 18 .8 18 .1 20 .2 22.3 42 .8 43 .2 78 .5 61 .5 117 .4 91 .0 . <LOQ a.oQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ 2.7 <LOQ 3 .6 2.4 Tlhursdcy, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -153- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA in Soils Treated with C 'T' Soil Type Alfisol __ Sample Interval Day 0 Week 1 week 2 Week 4 . Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live SYaFle <LOQ <LOQ 8 .5 8 .2 11 .4 11 .0 14 .9 15 .8 23.2 25.0 32.0 33 .1 58 .4 52 .5 97 .9 93 .7 65 .4 84 .2 133 .1 103 .5 194 .4 155.9 196.5 190.7 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 3 .3 <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ 9 .4 10_9 14_1 15 .4 19_7 19 .8 17.9 31 .1 90 .0 77 .8 99 .3 83 .5 <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.0 <LOQ 2.2 <LOQ LOD =limit of detection (0 .206 Rg/1cg) LOQ = limit of qvantitation (2 .0 ggllcg) Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 154 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (gg/kg) in Soils Treated witbC Soil Type- Sample Interval . Aerobic Live Sterzle Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ Q,OQ 18 .9 20.8 28 .6 29.0 55 .7 54.7 122_2 97.7 190.4 189.4 264.7 250.4 288.5 339.5 367.8 350.0 402.3 385.3 422.5 446.5 530.9 554.1 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.1 <LOQ 2.8 <LOQ 4.4 <LOQ 5 .6 3 .0 6.2 2.8 4.2 2.5 LOD = limit of detection (0 .206 Wg/kg) LOQ = limit of quantitation (2 .0 Rg/kg) Anaerobic Live SYuile <LOQ <LOQ 20 .9 17 .7 26 .5 26 .8 75 .5 48 .1 138 .0 144_3 284.5 302.6 327.0 347.1 <LOQ <LOQ <LOQ Q,OQ <LOQ 4,OQ _ <LOQ <LOQ <LOQ <LOQ 5 .0 <LOQ 7 .6 <LOQ Friday, Oaober 22, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -155- p. 156 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTUA (p.g/kg) in Soils Treated wi<, ] Soil Type Ultisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOD <LOQ Q,OQ <LOQ 2.1 2 .5 4 .0 3 .2 6.3 6 .7 12 .3 12 .7 12 .1 19 .3 30 .9 19.7 6.0 7.6 11 .3 13 .5 15 .6 6 .4 15 .6 32.3 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 4,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD Q.,OQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0.119 Vg/kg) LO Q =limit of quantitation (2.0 ~Lg/kg) Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -156- Project No.: 112E-108 Wildlife Intemational, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTUA (Ag/kg) in Soils Treated withc Soil Type Mollisol Sample Interval Day 0 week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live - Sterile <LOQ <LOQ <LOD <LOD <LOD <LOD <,OQ Q,OQ <LOQ Q,OQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ 3 .0 <LOQ <LOD <LOD <LOD <LOD <LOD Q .OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <,OQ <LOQ Q,OD QAD Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <L.OQ <LOQ Q..OQ Q .OQ <LOQ <LOQ <LOQ <LOD <LOD <i.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.,OQ <LOD <LOD LOD = limit ofdetection (0 .119 gg/icg) LOQ =lunit of quantitation (2 .0 jig/kg) Friday, Odober 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -157- p. 158 Project No . : 112E-108 Wildlife International, Ltd. Project Number 112E-308 Measured Concentrations of 8-2 FTUA (pg/kg) in Soils Treated witht Soil Type Alfisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic --- Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <I,OQ <LOQ <LOQ <LOD <LOD <LOD <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LQQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Q,OQ Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOQ 3 .9 2.2 2.3 <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ LOD =limit of dctection (0.119 pg/lcg) LOQ = Emit of quantitation (2 .0 ug/kg) Friaay, oeroe~ 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -158- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Soil Type Incentisol Measured Concentrations of 8-2 FTUA (wg/kg) in Soils Treated with C Sample Interval Aerobic Live Sterile Anaerobic Live Sterile Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD Q.OD <LOQ Q,OQ 5 .9 5 .0 25 .2 12 .3 77 .7 88 .9 164 .0 160.4 192.1 161 .0 209.5 206.9 225 .6 252.1 256.2 242.7 248.5 239.0 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 2 .7 <LOQ 2 .2 <LOQ <L.OQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ 7 .7 <LOQ 5.6 5.5 13 .0 13 .0 15 .4 10.0 <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOQ <LOQ . LOD = limit of detection (0 .119 pg/kg) LOQ =limit ofquantitation (2 .0 pg/kg) Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -159- p. 160 Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Soil Type ot Measured Concentrations of 8-2 FTA (gg/kg) in Soils Treated with c Sample Interval Aerobic Live Sterile Anaerobic Live Sterile Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOQ <LOQ 3 .8 3 .7 6.2 4.7 8.6 8 .5 9 .7 8 .0 10 .1 19 .0 19 .7 12 .0 3 .0 4.4 4.3 3.8 6.7 2.9 4.7 5 .2 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD 2.0 <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (0 .503 jig/kg) LOQ =limit of quantitation (2 .0 Ag/kg) Friday, ocroaa tz, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, .Ltd. -160- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-I08 Measured Concentrations of 8-2 FTA (pg/kg) in Soils Treated with[ Soil Tpe - Mollisol Sample Interval . Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile Anaerobic Live Sterile <LOD <,OQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD Q,OQ 2.3 <,OD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD<LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD Q,OD <LOD LOD =limit of detection (0 .503 gg/kg) LOQ =limit of quantitation (2 .0 pg/kg) Friday, Ottober 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -161- p. 162 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-1Os Measured Concentrations of 8-2 FTA (Ag/kg) in Soils Treated with C Sample Soil Type - - - Interval A1Sso1 Day 0 Wxkl Week 2 Week 4 . Month 2 Mouth 4 " Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of deteation (0 .503 gg/kg) LOQ = lanit of quantitation (2 .0 p&/kg) Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <,OD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Friday, Ociober l2, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -162- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Soil Type Incentisol Measured Concentrations of 8-2 FTA (Fcg/kg) in Soils Treated with(- Sample - -- Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic - .Live Sterile <LOQ <LOQ <LOD <LOD <LOQ <LOQ 5 .5 4 .8 18 .0 13 .0 73 .7 75 .7 162 .8 144 .6 163 .8 126.8 133.2 140.6 193.0 205.7 213.7 226.5 202.5 221.7 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 2 .9 <LOQ 2.8 Q,OQ <LOQ Q.OQ Anaerobic Live SYerile <LOD <LOD <LOQ <LOD <LOD <LOD 6.6 <LOQ 2 .5 2.6 6 .1 5 .9 8 .5 5 .3 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOQ LOD =limit of detcction (0.503 pg/kg) LOQ = limit of quantitation (2 .0 Wg/icg) Friday, Odober 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -163- p. 164 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFNA (ug/kg) in Soils Treated withC . Soil Type - Ult;so[ Sample Interval Day o Week 1 Week 2 Week4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ <LOQ 7.0 7.2 11 .5 12 .3 17,6 16 .7 24 .0 21 .5 28 .0 30 .0 28 .5 30 .7 33 .2 30.1 8.6 15 .7 23 .8 26.2 29.8 24 .4 31 .0 29 .5 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ Anaerobic Live Sterile <LOQ Q.OQ . 6.5 7.0 9.2 9 .5 15 .7 13 .7 15 .4 21 .5 23 .4 22_4 30 .0 25.5 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =limit of detection (0.124 I&kg) LOQ =limit of qvantitation (2 .0 g&/kg) Friday, October I$ 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -164- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Sail Type Mollisol Measured Concentrations of PFNA (jig/kg) in Soils Treated with I Sample Interval Day 0 W=k 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ <LOQ 5 .8 6.0 5 .7 8 .3 10.6 12.3 18 .1 17 .2 22 .4 22 .5 21 .2 20 .0 17 .3 11 .1 19 .5 18 .4 14 .6 25 .8 20 .7 17 .1 25 .7 34 .7 <LOD <LOD <LOQ <LOQ Q.OD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ d.OQ Q.OQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterfle <LOQ Q,OQ 4 .2 4 .3 5 .8 4 .6 4,3 5 .9 6 .5 8 .2 12 .1 9.3 16 .0 12 .1 <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ Q .OQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ LOD =1umt ofdetection (0 .124 W)lcg) LOQ =limit ofqaantitation (2 .0 jig/kg) Friday, Oc(obtr I2, 2007 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -165- p. 166 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFNA in Soils Treated with C g/kg) Soi1 Type Alfisol Sample Interval ' Day 0 Week 1 Week 2 Waok 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ <LOQ 3.3 4.0 4.6 3.9 6.6 7.7 10 .4 16 .2 10 .6 13 .5 8 .9 15 .9 17.6 23 .3 13 .4 17 .5 23 .1 15 .2 15 .7 17 .7 25 .3 22 .0 <LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ d,OQ d,OQ <LOQ <LOQ <LOD Q,OQ <LOQ <LOQ Q,OD <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ 2.4 2.7 3 .2 4.8 4.9 3 .6 6.6 8 .9 9.2 11 .2 7 .9 <LOQ <LOQ <LOQ <LOD <LOQ <LOD Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =limit of detection (0 .124 pg/Icg) LOQ =limit ofquantitation (2 .0 pg/kg) Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -166- Project No .: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFNA (,ug/kg) in Soils Treated withc Soil Type Tnccvtisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterik <LOQ <LOQ 20.3 25 .1 29.0 33 .4 36 .9 35 .2 62 .2 63 .9 59 .4 61 .9 67 .9 79 .9 79 .9 79 .5 71 .5 70 .7 73 .9 74.1 79.2 80 .6 96 .5 104 .2 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <I.OQ Q~OQ <LOQ <LOQ LOD =limit of detection (0 .124 gg/kg) LOQ =limit of quantitation (2 .0 jigJkg) Anaerobic Live Sterfle <LOQ <LOQ 9 .5 7 .2 13 .1 13 .5 34 .9 21 .8 24 .8 26 .6 28 .7 35.7 34.0 36.1 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ F.cday, ocreau 12, 2ooz Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -167- p. 168 Project No .: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (ILg/kg) in Soils Treated with[ Soil Type Ultisot Sarnpk Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 . month 18 Month 24 LOD =limit ofdatection (0.114 g&/kg) LOQ = limit of quantitation (2 .0 pg/kg) Aerobic Live SYa'ile <LOQ <LOQ 4.6 4.8 7.6 8.4 10 .7 11 .9 18 .0 18 .5 26 .6 28 .2 30 .4 35 .5 37.9 39 .3 9.2 20 .9 33 .3 34 .0 48 .1 36 .5 50 .7 44 .3 <IAQ <LOQ <LOQ <LOQ <LOQ <LOQ -COQ <LOQ <i.OQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ 4.8 5 .2 6 .9 7 .3 9.3 8.5 13 .2 15 .0 25.4 28.4 21 .9 19.6 <LOQ <LOQ <LOQ <.OQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ Aw.*day, woVemaer 08,2W7 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -168- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (gg/kg) in Soils Treated withc Soil Type Mollisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ Q .OQ 3 .4 3 .7 4.7 5 .2 6 .2 6 .2 9.9 10 .1 14_6 16.4 17.3 17 .6 16 .6 12 .4 19 .7 18 .6 14 .6 27 .7 22 .0 21 .4 45 .4 48.5 <LOQ 2LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.0 <LOQ Q.OQ <LOQ <LOQ 2.1 . <LOQ <LOQ LOD =limit of detection (0.114 pg/kg) LOQ =limit of quantitation (2.0 Ag/lcg) Anaerobic Live Sierile 4.OQ <LOQ 3 .7 3 .7 5 .4 4_7 6 .5 6 .7 11 .0 10 .7 17 .4 14 .2 21 .0 17.5 <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ . <LOQ <LOQ <LOQ <LOQ Thursday, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -169- p. 170 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (jLglkg) in Soils Treated with( Soil Type Affisol Sample Interval Day 0 Woekl Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ <LOQ <LOQ 2.1 2.9 3.0 4.4 4.8 8.1 9 .2 8.4 9.8 9.3 12.5 17.1 19 .7 12.9 16.1 26 .3 15 .3 23.3 20 .7 37 .6 36.8 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD Q.OQ <LOQ Anaerobic Live Sterile <L0Q <LOQ <LOQ 2 .4 3 .1 3.6 5 .4 5.6 4 .8 8 .7 12 .9 13 .2 14 .6 12 .2 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =lunit of detection (0 .114 gg/lcg) LOQ =limit of quanttation (2 .0 pg/ko Friday, Octabu 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -170- Project No. : 112E-108 41Tildlife.l'nternational, Ltd. Project Number I 12E-108 Measured Concentrations of PFDA (ug/kg) in Soils Treated witbC Soil Type Inceotisol Sampk Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile q,OQ Q.OQ 4.5 5.4 8.1 8.3 15 .6 15 .6 34 .3 29 .8 43 .5 50 .4 52 .3 68 .8 66 .7 57.2 62.0 69.8 62 .9 66.0 81 .4 77 .9 86 .7 ` 89 .6 <LOQ <LOQ <LOQ Q.OQ Q.OQ <LOQ <LOQ <LOQ 2 .1 <LOQ 2.6 <LOQ 3 .1 3 .1 2.9 2 .3 2 .3 2 .0 LOD =limit of deection (0.114 ;ig/lcg) LOQ =1unit of qvantitation (2 .0 Wg/lcg) Anaerobic Live Sterile <LOQ <LOQ 5.3 4.5 7.5 7.8 20 .9 11 .5 21 .4 22 .9 33 .7 39 .3 47 .0 41 .0 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2 .7 <LOQ Friday, Odobo 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -171- p. 172 Project No .: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA ("/kg) in Soils Treated wi Soil Type Ulfisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile Q,OQ <LOQ <LOQ <LOQ 3 .0 3 .3 5 .1 4 .7 8 .5 8 .0 13 .2 15 .0 16 .0 16 .4 18 .8 17 .0 3 .6 10 .5 15 .1 15 .5 19 .2 16 .1 22 .5 22 .7 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ Anaerobic Live SlerUe <LOQ <LOQ <LOQ <LOQ 2 .7 2 .6 4 .1 3 .9 6 .9 8 .2 15 .5 13 .1 18 .2 17 .6 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ ' LOD = limit of detection (0 .128 Wg/kg) LOQ = limit of quantitation (2.0 ~L81kg) Frlday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -172- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Soil Type Mollisol Measured Concentrations of PFUA (p.g/kg) in Soils Treated with I Sanple Interval Aerobic Live Sterile Anaerobic Live Stefile Day 0 Week 1 Week 2 Week 4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ 2 .1 2.7 2 .7 6.0 5 .7 10 .0 10 .8 9 .5 9 .6 8 .2 4 .6 8 .8 8 .6 6 .1 11 .6 10 .4 9 .2 14 .5 21 .3 <LOQ <LOQ <LOD <LOD <LOD <LOD 4.OQ <LOQ <LOQ Q.OD Q.OQ Q.OD <LOQ <LOQ <LOQ Q,oQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2 .0 2.5 3.5 6.0 4.7 6.8 5.5 <LOQ <LOQ <LOQ <LOQ <LOD <LOD Q.OQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOD LOD = limit of detection (0.128 Wg/lcg) LOQ = limit of quantitation (2.0 gg/kg) Friday, oaoba12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -173- p. 174 Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA (ug/kg) in Soils Treated witbL Soil Tvke AlBsol Sample Interval ' Day 0 Week l Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOD <LOD <LOQ <LOQ <LOQ <LOQ 2 .0 2 .3 4 .6 6 .1 4 .1 6 .2 3 .2 6 .2 7 .3 11 .3 7 .6 9 .2 13 .5 5 .8 7 .0 7 .2 14 .4 10 .1 <LOD <LOD <LOQ Q.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Q,OD Q.OD <I,OQ <LOQ <L,OD <LOQ <LOQ Anaerobic Live Sterile <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 3 .0 3 .1 3 .3 4 .5 3 .5 <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOD Q.,OD <LOQ <LOQ LOD = limit of detection (0 .128 gglkg) LOQ = limit of qnantitation (2.0 ~Lg/kg) Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -174- Project No. : 112E-108 Wildlife International, Ltd. Project Number 1 12E- 108 Soil Type Inceotisol Measured Concentrations of PFUA ( in Soils Treated with(- . Sanple Interval Aerobic Live Sterile /kg) Anaerobic Live SYerile Day 0 Week l Woek 2 W=k 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <,OQ <LOQ <LOQ 2 .4 3 .9 4.3 7.1 6.0 19 .0 19 .8 18 .7 18 .8 20 .8 28 .0 29 .4 28 .2 25.9 29.3 26 .1 25 .7 34 .9 33 .0 40 .5 45 .3 <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ Q.OQ <LOD <LOD <LOQ <LOQ 3 .4 3 .5 9.6 6.3 14.1 14.2 17.1 19.1 22 .2 21 .1 <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ Q,OD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ LOD =limit of detection (0 .128 gg/kg) LOQ = limit of quantitation (2 .0 ~Lg/kg) Friday, Oacbn 22,2#07 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -175- p. 176 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (pg/kg) in Soils Treated with [ Soil Type Uwso1 Sample Interval Day 0 Week 1 Weak 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <.OQ <LOD <.,OQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =linrit of detection (22.3 RgJkg) LOQ =limit of quantitation (100 ~Lg/kg) Monday, Octobo 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -176- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Soil Type Mollisol - Measured Concentrations of 8-2 FTOH in Soils Treated with C r) Sample Interval Day 0 Week l Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 1_2 Month 15 Month 18 Month 24 Aerobic --- Anaerobic Live Sterile Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <L.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Q.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q .OD <LOD <LOD <LOD <LOD LOD = limit of detection (22.3 ggAcg) LOQ = limit of quantitatioai (L00 4kg) Monday, Odober 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -177- p. 178 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (pg/Icg) in Soils Treated withc Soil Type Alfisol Sample Interval Day 0 Week l Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Mouth 15 Month 18 Month 24 Aerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (22.3 gg/kg) LOQ =1mmit of quantitation (100 jig/kg) Monday, Odobo I5, 2007 Company Sanitized . Does not contain TSCA CBI. Wildlife International, Ltd. -178- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Soil Type Incentisol Measured Concentrations of 8-2 FTOH (gg/kg) in Soils Treated with ~_ Sample Interval Day 0 Week i Wwk2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Q.OQ 174 125 148 138 <LOQ 140 100 <LOQ 131 165 173 121 <1,OQ <1.OQ <LOQ <LOQ 4,OD <LOD <LOD d,OD Q,OD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterfle <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (22,3 ug/k6 LOQ =limit of quantitation (100 KgW Monday, October 1S, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -179- p. 180 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (p .gIL) in Headspaces Gases from Chambers Treated with E Soil Type Sample _ Interval Aerobic Live Sterile Ultisol Day o Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q. .OQ <LOQ <LOQ <LOQ <L .OQ - <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (12.5 gg/L of headspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 180 - Project No.: 112E-108 Widdlife International, Ltd. Project Number 1i2E-ios Measured Concentrations of 8-2 FTOH (pLg/L) in Headspaces Gases from Chambers Treated with[ Sample Soil Type - - interval Aerobic Live Sterile Mollisol Day 0 Week 1 Week 2 W=k 4 Month 2 Month 4 Month 6 . Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 4,OQ Q.OQ <LOQ <LOQ <LOQ Q,0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (12 .5 Etg/L of hcadspace) Anaerobic Live Sterile Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ Q,OQ <LOQ <L.OQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ 4,0Q <LOQ <LOQ <LOQ Monday, October Is, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 181 - p. 182 Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (p.g/L) in Headspaces Gases from Chambers Treated with C J Soil Type Alfisol Sample Intwal Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ < .0Q <LOQ <LOQ <AQ <LOQ <,OQ <LOQ <LOQ <LOQ < .0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ LOQ = limit of quantitation (12.5 n/L of headspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 ------------- - 182 - Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (p.g/L) in Headspaces Gases from Chambers Treated with f- Soil Type Sample Interval Aerobic Live Sterile Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 ' Mouth 6 Month 9 Month 12 Month 15 Month 18 Month 24 Q,OQ <,OQ <LOQ Q,OQ <LOQ <,OQ <,OQ <LOQ QAQ <LOQ <LOQ <,OQ <LOQ <.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q OQ Q,OQ <LOQ <LOQ <1OQ <,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <1,OQ <LOQ <LOQ<LOQ <LOQ <LOQ <LOQ 1 .-Q LOQ = limit of quantitation (12.5 WglL ofheadspace) Anaerobic Live Sterile <LOQ <LOQ <,OQ <LOQ <LOQ <L,OQ <LOQ <L,OQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ U.OQ <LOQ <LOQ <LOQ <IAQ <LOQ <LOQ <LOQ <LOQ Monday, Odobcr 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -183- p. 184 Project No.: 112E-108 Wildlife International, Ltd. Project Number 1121r108 Measured Concentrations of 7-2 s FTOH (pgJkg) in Soils Treated with[, Soil Type Sample interval Aerobic Live dYerile Ultisol Day0 . Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 , Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ 133 .0 166 .1 <LOQ <LOQ <.OQ <LOQ 115.6 <LOQ <LOQ <LOQ <LOQ LOQ =limit of quantitation (100 n/kg) Anaerobic Live SYerile Tkarsday, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -184- : .~- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (gg/kg) in Soils Treated with(' Soy Type Sample Interval Aerobic Live Sterile Mollisol . Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <.OQ <LOQ <LOQ <LOQ Q <LOQ <LOQ Q,OQ <LOQ LOQ = limit of quantitation (100 ug/kg) Anaerobic Live Sterile Tihursday, November 08, 207 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -185- p. 186 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (p.g/kg) in Soils Treated withc, Soil 7)pe t Sangrte _ Interval Day 0 Month 2 Month 6 Month 9 Month 12 . Month 15 Month 1s Month 24 Aerobic Live bYerile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ <LOQ Anaerobic Live Sterile LOQ =limit of quantitation (100 gg/lcg) rh~day, November oa, 200 7 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 186 - Project No . : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (Ftg/kg) in Soils Treated withC Soil Type Incentisol Sample Interval Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile q,OQ Q.OQ <j,OQ <LOQ 278.3 278.3 192,8 464.2 193 .0 118 .9 173 .2 182 .2 <LOQ Q,OQ Q,OQ <LOQ LOQ =limit of quantitation (100 jig/kg) Anaerobic Live Sterile 27wrsday, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 187 - p. 188 Project No .: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-3 Acid (Wglkg) in Soils Treated witlC Soil Type Uttisol sample Interval Day o Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Aerobic Live Sterile <LOQ Q.OQ 6.6 9.1 16 .3 21 .4 31 .3 28 .7 41 -7 33 .4 34.1 40 .1 27.8 17.0 54.3 56 .2 Anaerobic Live Sterile LOQ =limit of quantitation (2 .0 Wg/kg) Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -188- Project No.: 112E-108 Wildlife International, Ltd, Project Number I1zE-108 Soil Type Mollisol Measured Concentrations of 7-3 Acid (tu.g/kg) in Soils Treated with E Sample Interval Aerobic Live Sterile Anaerobic Live Sterile Day 0 Month 2 Month 6 . Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ 2,4 2.7 2_7 2.9 5 .0 4 .8 6 .6 9 .0 3 .5 3 .4 8 .9 6 .6 LOQ =1mmit of quantitation (2.0 gg/lcg) wedmaday, ararck Z2,2008 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -189- p. 190 Project No.: 112E-108 Wildlife International, Ltd, Project hTurnber 112E-108 Measured Concentrations of 7-3 Acid (p.g/kg) in Soils Treated withC Soil Type Affiso1 Sa-ple Interpal Day 0 Mouth 2 Month 6 Month 9 Month 12 Month 15 Month 1 S Month 24 Aerobic Live Sterlie <LOQ <LOQ Q.OQ ~LOQ 3 .2 2 .5 3 .2 2 .5 4.9 6.1 7.6 8.9 8.1 6.4 6.6 7.3 Anaerobic Live Sterile LOQ =limit of quantitation (2.0 jig/kg) Wednerday, Marc* I7, 2008 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 190 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-3 Acid (1%g/kg) in Soils Treated with(- Soil Type Inceotisol S-Ple InYervar Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOQ =limit of quantitation (2.0 Wg/kg) Aerobic Live Sterile <I,OQ <LOQ 16 .7 9.6 67.7 81 .6 128 .3 153 .2 277.0 246.2 260.7 289.7 282.3 212 .2 355 .2 321 .9 Anaerobic Live Sterile Wednesday; March I2, 2008 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -191- p. 192 Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of PFOA (pg/Izg) in Untreated and Fortified Soils Soil Type Smnple Interval Untreated Controls Aerobic Anaerobic U1tisn1 Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ d,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =limit of detection (0 .206 gg/kg) LOQ =limit of quantitation (2 .0 llg(kg) Fortified Recoveries Aerobic Anaerobic 9 .5 95% 7 .8 78% 8 .6 86% 9 .1 91% 9 .2 92% 9 .2 92% 10 .4 loa% 10 .3 103% 12 .0 120% 11 .4 114% 10 .6 106% 10 .4 104% 9.2 92% 10 .0 loo% 7.4 74% 6.8 ss% 9.5 95% 9.2 92% 10 .5 105% 11 .3 113% 9.2 92% 9.8 98% 9.3 93% 9.0 90% 11 .4 114% 10 .8 loa% 11 .4 114% 11 .3 113% 11 .2 112% 11 .5 115% Friday, Octobar 12, 2007 Page 1 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -192- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFOA (pg/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 Weak 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD q,OQ . <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (0 .206 Ilg/kg) LOQ = Iialit ofquantitation (2 .0 uglkg) Fortk;fied Recoveries Aerobic Anaerobic 8 .9 9 .3 9 .7 10 .0 8.8 9.4 9 .1 9 .0 10 .7 11 .2 13 .2 12 .1 13 .1 12.0 11 .4 11 .6 9 .2 10 .4 89% 93% 97% loo% 89% 94% 91% 90% 107% 112% 132% 121% 131% 120% 114% 116% 92% l04% 9.3 10_1 8.5 9.6 8.5 8 .9 9.5 9.9 12 .2 10 .9 14 .0 13 .9 14 .5 14 .3 93% 101% 83% 96% ss% 89% 95% 99% 122% 109% 140% 139% 143% 143% Friday, October 12, 2007 Page 1 ofd Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -193- p. 194 Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of PFOA (p.g/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic l Day 0 <L.OD Week 1 <LOD <LOD Week 2 <LOD <LOD <LOD Wk 4 <LOD <LOD Month 2 <LOD <LOD Month 4 <LOQ Q,OQ Month 6 <LOD <LOD Month 9 <LOD <LOD Month 12 <LOD <LOD Month 15 <LOD Month 18 <LOD Month 24 Q.OD Y.OD =lunit of detection (0 .206 pg/icg) LOQ =limit of quantitation (2 .0 jig/kg) <LOD <LOD <LOD Q,OD <LOD <.OD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD Forh'fied Recoveaes Aerobic Anaerobic 9.2 92% 8.3 83% 8 .9 89% 10.0 ioo% 9.9 99% 9.4 94% 10 .0 100% 10 .1 lol% 11 .0 110% 10 .7 lo"r% 10 .8 109% 11 .5 lls% 11 .0 130% 11 .1 ul% 11 .5 115% 12 .5 125% 9~2 92% 9.3 93% 9.2 8.2 8.7 9.3 9.6 10 .0 10 .6 10.4 10.4 10 .7 11 .1 10.9 10.5 11 .2 92% 82% 97% 93% 96% 100% 106% 104% 104% 107% 11176 109% 103% 112% Friday, October 12, 2407 Page 3 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 194 - Project No. : 112E-108 Wildlzfe International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of PFOA (Wg/kg) in Untreated and Fortified Soils Soil Type Sampde Interval Untreated Controls Aerobic Anaerobic Inceotisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ Q.OQ q,OQ <,OQ <LOQ <,OQ <,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =limit ofdetection (0 .206 tlg/kg) LOQ =limit of quantitation (2.0 jig/kg) Forh'fied Recoveries Aerobic Anaerobic 10 .4 10 .5 12 .9 10 .1 10 .6 10 .9 12 .4 11_2 11 .3 11 .7 13 .4 10 .8 12.1 10.6 14.5 12 .4 9 .6 9 .6 104% los% 129% lol% l06% 109% 124% 112% 113% 117% 134% los% 121% 106% 145% 124% 96% 96% 9 .7 9 .4 10.9 9 .8 10.8 10.5 10.7 10.9 11 .9 11 .1 10.6 11 .5 11 .3 11 .6 97% 94% 109% 98% loa% los% 107% 109% 119% 111% 106% 115% 113% 116% Friday, ouab~ .t2, 2007 pag, 4 ofs Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -195- p. 196 Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (Wg/kg) in Untreated and Fortified Soils Soil Type Ultisol Sample Intcrval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortif=ed Recoveries Aerobic Anaerobic 7.2 72% 6.5 65% 7.7 77% 7 .9 79% 6_9 69% 6 .9 69% 7 .2 72% 7 .2 72% 8 .9 s9% 8 .0 so% 6 .5 65% 7 .3 73% 2.9 29% 5.4 54% 5.1 51% 4.0 40% 8.0 so% 7.4 74% 7.7 77% 8.7 87% 8.4 94% 8.2 82% 8.2 92% 7.9 79% 9.3 93% 7.6 76% 8.2 82% 8.0 sosc 6.5 65% 7.0 70% LOD =lunit of detection (0 .119 wg/kg) LOQ = limit of quantitation (2 .0 ~Lglkg) Friday, Odober 12, 2007 Page 1 nfd Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 196 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (p.g/kg) in Untreated and Fortified Soils Soil T1pe Mollisol Sample Inter9al Day 0 Week 1 Week 2 Week 4 Month 2 Mouth 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 . Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD LOD =limit o detection (0 .119 tlg/kg) LOQ =limit of quantitation (2 .O llg/kg) Fortified Recoveries Aerobic Anaerobic 9.2 9.0 8.1 8 .5 9.1 9 .5 9 .0 9 .1 8 .8 8 .7 10.0 9.4 10.2 8 .2 7 .8 9 .0 5 .9 6 .3 92% 90% 81% 85% 91% 95% 90% 91% n% 87% loo% 94% 102% s2% 79% 90% 59% 63% 7.3 8.8 7.7 8.7 7.7 9 .8 8 .0 8 .0 9 .7 7.8 9.9 10 .7 10 .0 8 .5 73% 88% 79% SR4 77% 98% so% 80% 97% 78% 99% 107% loo% 95% Friday, October 12, 2007 Page 2 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -197- p. 198 Proj ect No.: 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (p.g/k.g) in Untreated and Fortified Soils Soil Type AlSsol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month is Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Forn;fudRecoveries Aerobic Anaerobic 8 .4 94% 7 .7 77% 8 .6 86% 9 .8 98% 10 .5 105% 7.6 76% 7_5 75% 9.2 92% 9.6 96% 9.1 91% 6.6 66% 9 .0 90% 8 .4 84% 9 .0 90% 8.0 So% 11 .1 ttt% 6.2 62% 6.7 67% 8 .9 7 .5 9 .3 9 .5 9 .2 8 .9 8 .6 9 .4 10 .9 10 .8 8 .1 8 .3 10 .3 8 .3 99% 75% 93% 95% 92% 89% 96% 94% ]09% 10s% s]% 93% ]03% 83% LOD =limit of detection (0.119 Rg/tcg) LOQ =limit of quantitation (2 .0 pg/kg) Friday, Odobar 1.2, 2007 Page 3 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -198- Project No.: 112E-108 Wildlife Intemational, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (Icg/kg) in Untreated and Fortified Soils Soil Type Incentisol Sample Interval Day 0 Week I Week 2 Week 4 Mouth 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic -WD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD Q .OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic Anaerobic 11 .0 llo% 9.6 96% 10 .8 ]OS% 8 .3 83% 8 .8 sa% 9.7 97% 9.4 94% 7.9 79% 8.0 ao% 8 .9 09% 10 .1 lol% 8 .1 81% 7 .0 70% 6 .8 68% 7.4 74% 6.4 64% 5.3 53% 5.2 52% 7.9 79% 8.1 81% 8.3 83% 7_5 75% 8.5 85% 7.8 78% 7.7 77% 8.2 82% 7.6 76% 8.8 88% 6.6 66% 7.0 70% 6.1 61% 7.1 7155 LOD =lunit of detection (0.119 ~Lg/kg) LOQ = limit of quarititation (2.0 tlg/icg) Friday, October 12, 2007 pag. 4 of 4 Company Sanitized . Does not contain TSCA CBf . Wildlife International, Ltd. -199- p. 200 Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (pglkg) in Untreated and Fortified Soils Soil Type Ultisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOT) <LOD <LOQ <LOQ <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic Anaerobic 6.1 61% 5 .8 58% 6 .2 62% 6 .9 69% 5 .4 54% 5 .5 55% 5.4 54% 5.3 53% 3.3 33% 2.4 24% 3.4 3476 3.7 37% (1 .4) (1 .4) 4.1 41% (1 .7) 6_7 67% 6.6 66% 7.0 70% 6.9 69% 6.1 61% 6.5 65% 5.3 53% 6.3 63% 7.2 72% 5.1 51% 4.8 49% 4.6 46% 3.5 35% 3 .2 324'. LOD =limit of detection (0 .503 Eag/kg) LOQ =limit of quantitation (2 .0 pg/kg) Values in parentheses are <LOQ and >=LOD Friday, Octaber 12, 2007 Page 1 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -200- Project No. : 112E-108 Wildlfe International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (gg/kg) in Untreated and Fortified Soils Soil Type Moliis01 Sample Interval Day 0 a'e& 1 Weolc 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD Q.OD 2.7 <LOD <LOD <LOD <LOQ Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic " Anaerobic 8 .7 srx 9 .0 90% 8 .4 84% 9.0 90% 9.0 90% 8 .8 89% 8 .3 83% 8 .1 ei% 7 .8 78% 7 .9 79% 8,4 94% 6.9 69% 6.4 64% 6.2 62% 5.0 sos6 6.2 62% 2.3 23% (1 .7) 7.0 70% 8.7 87% 7.1 71% 7.6 76% 6.4 64% 8.3 83% 6.3 63% 7.0 70% 8.0 Su% 7.1 nss 5.8 59% 6.5 65/. 5.7 57% 5.2 52% LOD =lunit of detection (0 .503 gglkg) LOQ =limit of quantitation (2 .0 Kg/kg) Values in parentheses are <LOQ and >=LOD Friday, Odobar 12, 2007 Page 2 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -201 - p. 202 Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (pg/kg) in Untreated and Fortified Soils Soil Type -Alfisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <L .OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <T.OQ <LOQ Fortified Recoveries Aerobic Anaerobic 7 .8 78% 7 .9 79% 7 .8 72% 8 .4 94% 10 .6 106% 8 .7 87% 6 .8 68% 8 .4 s<% 7 .5 75% 7 .0 70% 4 .2 42% 6 .7 67% 6 .0 60% 6 .6 66% 5.1 51% 7.7 77% 4.0 40% 3.7 37% 8.8 as% 6.7 6796 8.5 85% 8.4 84% 8.4 94% 8.1 81% 7.7 77% 8.3 93% 8.8 98% 8.3 83% 6.7 67% 6.6 66% 7.3 73% 7.0 70% LOD =limit of detection (0 .503 gg/kg) LOQ =limit of quantitation (2 .0 Kg/kg) Values in parentbeses are <LOQ and >=LOD Friday, October 12, 2007 ' Page 3 of f Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -202- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (mg/kg) in Untreated and Fortified Soils Soil Type IneentisoI Sample Interval Day 0 Week 1 Week 2 weak 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD Q,OD <LOD <,OD <LOD <LOQ <LOQ <,OQ <LOD <LOD <LOD <LOQ <LOD <,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic Anaerobic 10 .7 9 .6 9.8 7.6 7.0 8 .4 7 .1 6 .3 5 .5 5 .8 5.3 4.5 4.3 3 .7 3 .4 3 .4 2.1 (1 .8) 107% 9s% 98% 76% 70% 84% 71% 63% 55% 5s% 53% 45% 43% 37^A 34% 34% 21% 7.8 78% 7.2 n% 7.0 70% 6.7 67% 7.2 72% 7.6 76% 5.9 59% 6.6 66as 4.8 48% 6.3 63% 3 .6 36% 3.4 34% 2.6 26% 3.5 35% LOD = limitofdetection (0 .503 Wg/kg) LOQ =lirnit of quantitation (2 .0 gglkg) Values in parentheses are 4,0Q and >=LOD Friday, oaob~ 12 2007 PW 4 ofe Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -203- p. 204 Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (gg/kg) in Untreated and Fortified Soils Soil Type Ultisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ LOD =lunit of detection (0 .124 uglkg) LOQ =limit of quantitation (2 .0 jig/kg) Fortied Recoveries Aerobic Anaerobic 9 .4 7 .8 9.7 9.8 9.5 9.8 10 .4 10 .1 11 .4 11 .4 10.3 10 .6 7 .0 10 .3 7.9 6 .7 94% 79% 97% 98% 95% 98% 104% lol% 114% 114% 303% 106% 70% 103% 79% 67% 10 .0 9 .9 9.9 10.7 9.7 10 .3 10 .3 9.6 11 .2 11 .0 11 .6 11 .7 10 .8 11 .6 300% 99% 99% 107% 97% 103% 103% 112% 130% 116% 317% 108% 11696 Friday, October 22, 2007 Page I of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 204 - Proj ect No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (p.g/kg) in Untreated and Fortified Soils Soil Type Mollisol Sample Interval Day 0 Week 1 Weak 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ QAD <LOD <LOD <LOD <LOD <LOD <LOQ Q,OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortif ed Recoveries Aerobic Anaerobic 9.4 94% 9.6 96% 9.7 97% 9.7 97% 9 .5 95% 9.9 99% 10.2 ]02% 8.6 86% 10.7 107% 10.6 106% 12.2 122% 11 .9 119% 12.0 120% 11 .7 117% 10.0 100% 11 .2 112% 8.1 sl% 8.3 83% 9_6 96% 10.1 lal% 8 .9 99% 9.8 98% 9.6 96% 9.9 99% 9.3 93% 9.8 99% 10.7 107% 10.1 lol% 11 .6 116% 12.8 128% 12.2 122% 11 .9 119% LOD =limit of detection (0.124 4glicg) LOQ =lilnit of quantitation (2 .0 gglkg) Friday, Odaber 12, 2007 Page 2 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -205- p. 206 Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (pg/kg) in Untreated and Fortified Soils Soil Type Alfisol SampleInterval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month-12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD Q.OD <LOQ Q,OQ <LOQ <.OQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ LOD =1unrt ofdetection (0 . 124 1tg/kg) LOQ =limit ofquantitation (2 .O llglkg) Fortified Recoveries Aerobic Anaerobic 9 .8 9 .3 9.4 11 .2 10.3 9.1 10.3 10.7 11 .3 11 .0 10,0 11 .4 10 .8 11 .4 11 .3 12.3 8 .7 8 .6 98% 93% 94% 112% 103% 91% ]03% 107% 113% 110% 100% 114% los% 114% 113% 123% 97% 86% 9.7 8 .1 9.4 10.1 9.8 10 .1 10 .3 10 .0 11 .0 11 .0 10 .7 10.7 11 .5 10 .2 97% Si% 94% lol% 98%, 301% 103% loo% 110% 110% 107% 10?% 115% 302% Friday, October 12, 2007 pag. 3 al 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 206 - Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of PFNA (W/kg) in Untreated and Fortified Soils Soil Type _ Incentisol ,Sarnple Interval Day 0 Week ] Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <.OQ <LOQ Q.OD <LOD <LOQ <,OQ <LOQ <LOD <LOQ Q,OD <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <,OQ Q..OQ <LOQ <LOQ QJOD <LOQ . <LOQ <LOQ <LOQ Fordfied Recoveries Aerobic Anaerobic 12 .4 124% 11 .6 116% 13 .0 130% 10.6 !06% 10.7 107% 10.7 107% 12 .5 125% 10 .7 107% 11.2 112% 11 .9 119% 14 .0 140% 10 .5 105% 10 .4 104% 10 .0 100% 13 .0 lao% 10 .9 109% 8.9 99% 8.8 sa% 10.2 102% 10 .0 loo% 10 .8 los% 9.8 98% 10 .3 103% 10 .2 102% 10 .1 lol% 10 .7 lor% 11 .1 111% 11 .1 111% 11 .0 110% 10 .8 109% 10 .1 lolst 10 .8 loe% LOD = Gmit of ddaction (0 .124 gg/kg) LOQ =limit of quantitation (2 .O "/kg) Friday, October 11, 2007 Page 4 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -207- p. 208 Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of PFDA (jig/kg) in Untreated and Fortified Soils Soil Type Ultisol Sample interval Day 0 Week 1 Week 2 Week 4 Mouth 2 Month 4 Mouth 6 Month 9 Month 12 month 15 month 18 Month 24 Untreated Controls Aerobic Anaerobic <,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ Fortified Recoveries Aerobic Anaerobic 9.3 93% 7.4 74% 8.9 as% 8 .6 ss% 8 .8 88% 8 .9 E9% 9.4 94% 9.3 93% 10 .9 ]09% 10 .9 109% 9 .6 96% 10 .0 100% 5 .2 52% 9.5 95% 8.4 94% 7.0 70% 9.2 92% 9.1 91% 9.0 90% 9.7 97% 9.4 94% 9.2 92% 9.3 93% 8.0 so% 10 .4 104% 10 .2 ]02% 11 .0 1]0% 10 .9 109% 9.6 96% 10 .4 104% LOD =limit of dctection (0.114 ug/kg) LOQ =limit of quantitation (2 .O jig/kg) Friday, October 12, 2007 Page l oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -208- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (fLg/kg) in Untreated and Fortified Soils Soil Type Moltisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 , Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <,OD <LOD Q,OD <LOD d,OD Q,OQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <,OQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortifud Recoveries Aerobic Anaerobic 10 .0 100% 10 .0 100% 9 .8 98% 10 .4 104% 9 .7 97% 10 .1 lolsa 9 .8 98% 8 .1 sl% 9.9 99% 9.7 97% 11 .7 117% 10 .9 109% 12 .1 121% 11 .2 112% 9.2 92% 10 .2 102% 7.6 76% 8.0 80% 9 .2 9 .9 8 .2 9 .5 9 .2 9 .3 9.0 9.0 10.4 9.2 11 .4 12 .1 11 .2 10 .9 92% 99% 82% 95% 92% 93% 90% 90% 104% 92% 114% 121% 112% 109% LOD = limit ofdetection (0 .114 ~cg/lcg) LoQ =lirnitofqllantiration. (2 .0 Kgks) Friday, October 12, 2007 Pagn 2 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -209- p. 210 Project No. : 112E-108 Wildlife International, Ltd. Proj ect Numbers 112E-108,109,110 Measured Concentrations of PFDA (p.g/kg) in Untreated and Fortified Soils Soil Type- Alfisol Sample Interval Day 0 Wcek 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD Q.,OD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOQ ForlifliedRecoveries Aerobic Anaerobic 9 .3 93% 9 .3 93% 9 .4 94% 11 .0 110% 10 .1 101% 8 .6 86% 9 .0 90% 10 .5 105% 11 .9 119% 11 .2 112% 9.3 93% 10 .9 109% 10 .7 107% 11 .2 112% 10 .9 109% 13 .3 133% 9.2 92% 9.3 93% 9.2 92% 7.4 74% 8.7 87% 9.3 93% 9.0 90% 9.5 95% 9.7 97% 9.6 96% 11 .3 113% 11 .4 114% 9.0 90% 9.6 96% 11 .6 116% 8.6 86% LOD =limit of detection (0.114 ~Lg/kg) LOQ = limrt of quantitation (2.O llg/kg) Friday, Odobar 12, 2007 Par 3 of s Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 ---------- -210- Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils Soi1 Type Incentisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ Fortified Recoveries Aerobic Anaerobic 13 .9 12.4 14.6 11 .6 11 .3 11 .6 12 .1 11 .5 12 .0 13 .3 14 .1 10 .8 10 .7 9 .2 12 .8 10 .9 9.5 9 .2 139% 124% 146% 116% 113% 116% 121% lls% 120% 133% 141% los% 107% 92% 128% 109% 95% 9w. 9.9 9.7 10.7 9.6 10.8 10 .5 10 .1 10 .7 11 .8 12 .2 10 .8 11 .6 9.6 10 .6 99% 97% 107% 96% los% lOJ% 101% l07% 118% 122x los% 116% 96% 106% LOD =limit of detection (0 .114 ~Lg/kg) LOQ =limit of quantitation (2 .O llg/kg) Friday, October 12, 2007 Pagr 4 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 211 - p. 212 Project No.: 112E-108 Wzldlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (Wg/kg) in Untreated and Fortified Soils Soil Type Ultisol Sample IntQVat Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Mouth 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ d.OQ Q,OQ Fortified Recoveries Aerobic Anaerobic - 8 .5 6.9 8 .2 8 .2 7 .6 7 .8 8 .6 7 .4 10 .2 10 .1 9.2 9.3 4.1 8.4 7.2 6.2 95% 69% 92% 82% 76% 79% 86% 74% 102% 101% 92% 93% 41% 94% n% 62% 8.5 95% 8.8 88% 8.5 85% 8.4 84% 8.8 sa% 8.6 86% 9.1 91% 7.0 70% 9.8 98% 9.5 95% 9.4 94% 9.5 95% 8.8 se% 9.9 99% LOD = limit of detection (0 .128 ~lg/kg) LOQ = limit of quantitation (2 .0 Wg/kg) Fridcy, October 71, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 212 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,130 Measured Concentrations of PFUA (gg/kg) in Untreated and Fortified Soils Soil Type Mollisol ' Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Mouth 6 Month 9 Month 12 Month is Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ Q,OQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD LOD =lirnit of detection (0 .12811g/lcg) LOQ =limit of quantitation (2 .O llg/kg) Fortified Recoveries Aerobic Anaerobic 9 .5 9 .6 8 .7 9_3 9.0 9.2 8 .7 6 .9 8 .4 8 .4 10 .1 10 .1 9.5 8.7 7.4 8 .8 6 .1 6 .3 95% 96% 97% 93% 90% 92% 87% 69% 94% 84% lol% iol% 95% &7% 74% sa% 61% 63% 8.2 82% 8.6 86% 6.7 67% 7.6 76% 6.3 63% 7.1 71% 6.3 63% 8.0 80% 8.5 85% 7.5 75% 9.6 96% 9.4 94% 9.8 98% 7.9 79% Friday, ocrobe.l2, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -213 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (gg/kg) in Untreated and Fortified Soils soil Type Sarnple Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 <LOD <LOD <LOD <LOD Week 1 <LOD <LOD <LOD <LOD Week 2 <LOD <LOD <LOD Q.OD Week 4 <LOD <LOD Month 2 Month 4 <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD Month 6 . <LOD <LOD <LOD <AD <LOQ Q,OQ Month 9 <LOD <LOD Month 12 <LOQ <LOQ Month 15 <LOD Month 18 <LOD Month 24 <LOD LoD =1imit ofdctoction (0.128 pg/kg) LOQ =limit of quantitation (2 .0 Ftg/kg) Fortified Recoveries Aerobic Anaerobic 9.2 92% 8.7 97% 9.2 92% 10 .9 109% 9.4 94% 8.0 so% 7.7 77% 9.4 94% 10 .3 103% 9 .7 97% 7 .5 75% 8 .9 9.9% 8.9 99% 9.3 93% 8.6 96% 12.2 122% 8.7 sr.e 8.8 sa% 8.3 83% 6.1 61% 8.3 83% 8.6 96% 7.9 79% 7.9 79% 7.0 70% 7.6 76% 8.8 88% 9.9 99% 7.4 74% 7.2 72% 9.5 95% 6.7 67% Friday, Oaober 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -214- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (gg(kg) in Untreated and Fortified Soils Soil Type Incentisol Sample Interval Day o Week 1 W=k 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 , Untreated Controls Aerobic Anaerobic <LOQ <LOQ <,OD <LOD <LOD Q,OD <LOD <LOD <LOD <,OD <,OD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <..OQ <LOD <LOD <,OD <,OD <LOD <LOD <LOD <,OD <LOD <LOD <LOD <LOD <LOD <LOD <,OD <LOQ <LOQ Fortified Recoveries Aerobic Anaerobic 12 .0 120% 11 .2 112% 12 .5 125% 10.1 lol% 9.2 92% 10.3 103% 9.8 98% 8 .9 89% 10 .4 104% 11 .7 117% 12 .2 122% 8.8 sa% 8.2 8z% 8.3 83% 9.9 99% 8.2 92% 7.4 74% 6.9 69% 7.9 7.4 7.9 7.4 7.4 8 .7 8 .0 8 .5 8 .7 10 .3 6 .9 7 .3 7 .0 8 .5 79% 74% 79% 74% 74% 97% 80% 95% 97% 303% 69% 73% 70% es% LOD = limit of detection (0 .128 ~,g/kg) LOQ =limit of quantitation (2 .0 gglkg) Frid4q, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -215- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 F70R (Jag/kg) in Untreated and Fortified Soils Soil Type Ultisol Sample Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD -:3-OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Forh'fied Recoveries Aerobic Anaerobic 289 283 244 247 210 208 165 147 102 ( 98 ) ( 45 ) ( 53 ) ( 41 ) <LOD <LOD <LOD <LOD <LOD 115% 113% 97% 99% 84% 83% 66% 59% 41% 39% la% 21% 16%^ o% 0% o% 0% o% 291 288 220 258 116% 113% 98% 103% 249 99% 181 n% . 179 7156 155 62% 165 66% 117 47% ( 87 ) 35% ( 541 22% f 68 ) 27% LOD =limit of detection (22 .3 Ag/kg) LOQ =limit of quantitation (100 ilglkg) Values in parentheses are <LOQ and >=LOD Monday, October IS, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -216- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (gg/kg) in Untreated and Fortified Sofls Soil Type Mollisol Santple Interpal Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD Q,01) <LOD <LOD Q,OD <LOD QAD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic Anaerobic 299 120% 261 104% 213 85% 217 9M 194 78% 112 45% 119 49% ( 51 ) Ziu ( 63 ) 25% <LOD `K ( 32 ) 13% <LOD o% <LOD o% <LOD <LOD % <LOD <LOD % 266 279 136<LOD 12% 244 90% 273 109% 257 . 103X 243 97% 191 77% 192 77% ( 99 ) x' 162 65% ( 70 ) 29% ( 46 ) 18% ( 36 ) 14% <LOD % LOD =limit of detection (22.3 gglkg) LOQ = limit of quantitation (1001eg/lcg) Values in parentheses are <LOQ and >=LOD Monday, October 25, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 217 - p. 218 Project No.: 112E-108 Wildlife International, Ltd. Proj ect Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOR (pg/lzg) in Untreated and Fortified Soils Soil Type-- Alfisol Sanrple Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Forh'fied Recoveries Aerobic Anaerobic 307 245 210 143 156 142 117 112 ( 75 ) ( 79 ) ( 54 1 ( 51) <LOD ( 29 ) <LOD <LOD <LOD <LOD 123% 98% 84% 57% 62% 57% 47% 45% 30% 32% 22% 21% 0% 12% 0% 0% o% o% 267 254 215 203 205 161 127 143 ( 81 ) ( 62 ) ( 501 ( 371 <LOD <LOD 107% 102% 86% 81% 82% 64% 51% 57% 33% 23% 20% 15% o% 0% LOD =limit of detection (22 .311g/kg) LOQ = limit ofqaantitation (100 p.g/kg) Values in parentheses are <LOQ and >=LOD Monday, October 15, 2907 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -218- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (gg/kg) in Untreated and Fortified Soils Soil T)pe - - incmbsot Sample Interval Day a Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls Aerobic Anaerobic a.oD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD -*,OD Fortified Recoveries Aerobic Anaerobic 285 262 267 285 245 234 218 284 201 200 157 186 ( 86 ) ( 85 ) ( 55 ) 115 <LOD <LOD 114% 105% 107% 114% 98% 94% M 114% $l% 80% 63% 74% 34% 34% 22% 46% o% o% 287 285 25$ 260 237 235 230 219 134 165 106 106 ( 42 ) ( 37 ) 115% 114% 303% 104% 95% 94% 92% 87% 54% 66% 42% 42% 19% 15% LOD =limit of detection (22 .3 ilg/kg) LOQ =limit of quantitation (100 Etg/lcg) Values in parentheses are <LOQ and >=LOD aroneQy, oaobQls 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -219- p. 220 Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (pg/L) in Headspace Gases from Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Ultisol . Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ d,OQ <LOQ <LOQ <LOQ <LOQ <7AQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =limit of quantitation (12.5 pg/L of beadspacx) Forhfied Solls Aerobic Anaerobic <LOQ <LOQ d,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 1s, 2007 page 1 Of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 220 - Project No. : 112E-108 Wildlzfe International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH ()cg/L) in Headspace Gases from Untreated and Fortified Soils Soil Type Sample 1nterval Untreated Controls Aerobic Anaerobic MolIisol bay 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (12.5 Ixg/L of hoadspaca) Fortified Soils Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L .OQ <LOQ <LOQ <LOQ <LOQ d.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 15,26V7 Page 2 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 221 - Project No . : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (p,g/L) in Headspace Gases from Untreated and Fortified Soils Soil T~pe Sample Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ d,QQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitadiom (12.5 Ixg/L ofheadspace) Forttfied Soils Aerobic Anaerobic <LOQ <LOQ <LOQ Q .OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, Oclober IS, 1007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 222 - Project No. : 112E-108 ~ Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (p.g/L) in Headspace Gases from Untreated and Fortified Soils Soil Type - - Sample interval Untreated Controls Aerobic Anaerobic Incentisoi Day 0 Wcek 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <L0Q <LOQ C1,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ < .OQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =limit of quantitation (12.5 gg/L of headspace) Fortifzed Soils Aerobic Anaerobic <LOQ <JOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ . <LOQ <LOQ <LOQ Q..OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 15, 2007 Page 4 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. p. 224 Project No.: 112E-108 -223Appendix II Measured Concentration of Selected Analytes (pmoUkg Soil Dry Weight) Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -224- Project No.: 112E-108. Wildlife International, Ltd. Project Number 112E-1Q8 Measured Concentrations of PFOA (N.mol/kg) in Soils Treated with (, Soil Type Sample Interval Aerobic _ Live Sterile IAtisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0 .043 0 .046 0.077 0.088 0.140 0 .148 0 .295 0.362 0.651 0.903 0 .909 1 .065 1 .176 1 .349 0.888 0.831 0.821 0 .876 0 .992 0 .794 0.884 0.795 LOD =limit of ddection (0.0005 gmoUkg) LOQ =limit of quantitation (0.0048 pmollkg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0 .005 <LOQ 0.006 <LOQ 0.005 <LOQ 0.012 <LOQ 0.019 <LOQ <LOQ <.OQ Anaerobic Live Sterile <LOQ <LOQ 0.053 0 .055 0 .065 0 .058 0.124 0.097 0.169 0.177 0.277 0.322 0.359 0.253 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.006 <LOQ 0 .007 <LOQ 17mraday, November Q8, 2b07 . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -225 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (WnioUkg) in Soils Treated withL Soil Type Sample Interval Aerobic Live Sterile Mollisol Day 0 Week 1 Week 2 Week 4 - Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0 .037 0 .036 0.043 0.044 0.052 0_049 0.077 0.073 0 .111 0.142 0.180 0.180 0.223 0.167 0.251 0 .227 0 .238 0 .599 0 .307 0 .287 0 .554 0.501 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.007 <LOQ 0.008 0 .006 0.008 0 .008 0 .007 0.008 LOD = limit of detection (0.0005 WmoUkg) LOQ = limit of quantitation (0 .0048 pmol/kg) Anaerobic Live Sterile <LOQ <LOQ 0 .035 0 .036 0 .045 0 .044 0.049 0.054 0.103 0.104 0.189 0.149 0.284 0 .220 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ a.oQ <LOQ 0.006 <LOQ 0.009 0.006 Thursday, Navumber 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 226 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (p.mol/kg) in Soils Treated with c. Soil Type Sample Interval Aerobic Live Sterile A15sol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.020 0.020 0.027 0.026 0.036 0.038 0.056 0 .060 0 .077 0.080 0.141 0.127 0.236 0.226 0.158 0.203 0.321 0 .250 0.469 0.376 0.475 0.460 <,OQ <LOQ <LOQ <LOQ <,OQ <,OQ <LOQ <L.OQ <LOQ <,OQ <LOQ <LOQ <LOQ <L .OQ 0.008 <,OQ <LOQ <,OQ LOD =lunit of detection (0.0005 p,mol/kg) LOQ = limit of quantitation (0 .0048 prnol/kg) Anaerobic Live Sterile <LOQ <LOQ 0 .023 0 .026 0 .034 0 .037 0.048 0.048 0.043 0.075 0.217 0 .188 0 .240 0 .202 <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ Q,OQ <LOQ <LOQ 0 .005 <LOQ 0 .005 <LOQ Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 227 - Project No.: ~112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (p.mol/kg) in Soils Treated with C Soil Type Sample Interval Aerobic Live bYadle Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 _ Month 12 Month 15 Month 18 Month 24 <LOQ Q .OQ 0.046 0.050 0.069 0.070 0.135 0 .132 0 .295 0 .236 0 .460 0 .457 0 .639 0 .605 0 .697 0.820 0.888 0.845 0 .972 0 .930 1 .020 1 .078 1,282 1 .338 <LOQ <LOQ <LOQ <LOQ <LOQ QAQ 0.005 <LOQ 0 .007 <LOQ 0 .011 <LOQ 0 .013 0 .007 0 .015 0 .007 0.010 0.006 LOD = limit of detection (0 .0005 Eunollkg) LOQ = limit of quantitation (0 .0048 fnnol/kg) Anaerobic Live Sterile <LOQ <LOQ 0.050 0.043 0 .064 0 .065 0 .182 0 .116 0 .333 0.349 0.687 0.731 0.790 0.838 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0 .012 <L,OQ 0 .018 <LOQ Fridny, Octobo I2, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -228- Project No.: 112E-108 4 WildTife International, Ltd. Project Number 112B-108 Measured Concentrations of 8-2 FTUA (u.mol/kg) in Soils Treated with[ Soil Type Sanrple Interval Aerobic Live Sterile Ultiso[ Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD Q.OQ <LOQ <LOQ 0.005 0.006 0.009 0.007 0.014 0.015 0.027 0.028 0 .026 0 .042 0 .067 0 .043 0 .013 0 .017 0.025 0.029 0.034 0.014 0.034 0 .071 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD LOD =limit of detection (0.0003 gmoUkg) LO Q =limit of quantitation (0.0044 ftmol/kg) Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD Friday, October 12, 2007 Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. - 229 - p. 230 Project No .: 112E-108 WildlWe International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTUA (p.mol/kg) in Soils Treated withE Soil Type Sample Inlerpal Aerobic Live Sterlle MoDisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month is Month 24 <LOQ <LOQ <LOD Q,OD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ 0.007 <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOQ <LOQ <LOD <LOD LOD =limit of detection (0 .0003 EunoUkg) LOQ =limit of quantitation (0 .0044 punol/kg) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD 4,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD Friday, Odaber 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 230 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTUA (jr.moI/kg) in Soils Treated witht Soil Type Sample Interval Aerobic Live sterile Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 19 Month 24 <LOD <LOD <LOD <LOD <,OD <LOD Q,OQ <LOQ <LOQ < .,OD <LOD <LOD <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ LOD = limit of detection (0 .0003 p.moNkg) LOQ =limit of quantitation (0 .0044 pmol/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <,OD <LOD <LOD <LOD <LOQ <LOQ Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOQ 0 .008 0 .005 0 .005 <LOQ <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD d,OD Q..OQ Q.OQ Friday, Oclober I2, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 231 - p. 232 Project No. : 112E-108 Wildlife International, Ltd. ProjectNumber 112,E-los Measured Concentrations of 8-2 FTUA (P.mot/kg) in Soils Treated with[ Soil Type SamPle Interval Aerobic Live Staile Incentisol Day 0 Week 1 Week 2 Wk 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOQ <LOQ 0 .013 0 .011 0 .055 0 .027 0 .170 0.194 0.358 0.350 0.419 0 .352 0 .457 0 .452 0.492 0.550 0.559 0.530 0 .542 0 .522 LOD =limit of detection (0 .0003 FimoUkg) LOQ =limit of quantitation (0 .0044 Eunol/kg) Q..OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 0.006 <i.OQ 0.005 <LOQ <LOQ <LOQ Anaerobic Live 3terile <LOD <LOD <LOD <LOD <LOD <LOQ 0 .017 <LOQ 0 .012 0 .012 0 .028 0.028 0.034 0.022 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ x}iday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 232 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTA (gmoUkg) in Soils Treated withL Soil Type Sarxple Interval Aerobic Live Sterile Ultisol Day 0 Weak 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOQ Q,OQ 0.008 0.008 0.013 0.010 0.018 0 .018 0.020 0 .017 0 .021 0,040 0 .041 0.025 0.006 0.009 0.009 0.008 0.014 0.006 0.010 0.011 <I,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD _ <LOD <LOD <LOD Q.OD <.OQ Q.OD <LOD <LOD <LOD 0.004 LOD =limit of detectim (0_0011 pmoUW LOQ = Emit of quanntation (0 .0042 pmol/kg) Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD Friday, October 22, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 233 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTA (}cmoUkg) in Soils Treated withc Soil Type Sampk Interval Aerobic Live Stertk Mollisol Day 0 Week i VS'eck 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 month Is Month 24 <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD <LOD <LOQ 0.005 <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD ~ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (0 .0011 Funol/kg) LOQ =limit of quaatitation (0.0042 Eunol/kg) Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife Intemational, Ltd. - 234 - Proj ect No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTA (gmol/kg) in Soils Treated wnthC Soil Type Sanrple Interval Aerobic Live Sterile Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 , Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD Q.AD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ LOD =limit of detecrion (0 .0011 punollkg) LOQ = bunk of quantitation (0.0042 Nmol/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <,OQ <LOD <LOD <LOQ <LOQ <LOQ Q.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ FH&y, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 235 - p. 236 Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTA (pmolllcg) in Soils Treated with i~ Soil TypeInceotisol Sample Interva-l- --- ---Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month t 5 Month 18 Month 24 Aerobic ---_- .Live Sterile <,OQ <LOQ < .OD <LOD <LOQ <L.OQ 0 .011 0 .010 0_038 0 .027 0.154 0.158 0.341 0.302 0.343 0 .265 0 .279 0 .294 0 .404 0.430 0.447 0.474 0.423 0.464 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OQ <LOD 0.006 <L.OQ 0.006 Q.,OQ <LOQ <LOQ LOD =limit of detection (0 .0011 pmol/kg) LOQ =limit of quandtation (0 .0042 Funol/kg) Anaerobic Live Sterile <LOD <LOD <LOQ <.OD <LOD <LOD 0.014 <LOQ 0.005 0 .005 0 .013 0 .012 0 .018 0 .011 <LOD <LOD <LOD <LOD <LOD <,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <.OQ Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 236 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFNA (gmoUkg) in Soils Treated wittL Soil Type -- Sample Irrterval Aerobic Live Sterile Ultisol Day 0 week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 d.OQ <LOQ 0.015 0.016 0.025 0.026 0 .038 0 .036 0 .052 0 .046 0 .060 0 .065 0 .061 0 .066 0 .072 0 .065 0 .018 0 .034 0 .051 0 .057 0 .064 0 .053 0.067 0.064 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD = limit of detection (0 .0003 pmol/kg) LOQ = limit of quantitation (0 .0043 lunol/kg) Anaerobic Live Sterile <LOQ <LOQ 0.014 0.015 0.020 0_020 0.034 0.029 0.033 0.046 0.050 0 .048 0 .065 0 .055 <LOQ <LOQ <LOQ <LOQ <1.oQ a,OQ <LOQ <LOQ Q.OQ Q,OQ Q,OQ <LOQ <LOQ Q,OQ Friday, Odober 12, 2007 . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -237- p. 238 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFNA (Wmol/kg) in Soils Treated witbE Soil Type Sample Interval Aerobic Live Sterik Mollisol ' Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Q.OQ <LOQ 0.013 0.013 0 .012 0 .018 0 .023 0 .026 0 .039 0 .037 0 .048 0.048 0.046 0.043 0.037 0.024 0.042 0.040 0.031 0 .056 0 .045 0 .037 0 .055 0 .075 <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ LOD = limit of detection (0 .00031unoUlcg) LOQ = limit of quantitatioai (0 .0043 txmoUkg) Anaerobic Live Sterile <LOQ <LOQ 0 .009 0.009 0 .013 0 .010 0 .009 0 .013 0 .014 0 .018 0 .026 0 .020 0.034 0 .026 <LOD <LOD <LOQ Q,OQ <LOQ <LOD <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ Q,OQ FKday, October 12,2W7 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -238- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112.E-i08 Measured Concentrations of PFNA (}unoUkg) in Soils Treated with[ Soil Type Sample Interval Aerobic Live Sterile Alfisol , . Day 0 week 1 week 2 We& 4 Month 2 Month 4 Month 6 Month 9 Month 12 Mouth 15 Month 18 Month 24 <,OQ <LOQ 0 .007 0 .009 0 .010 0 .008 0.014 0.017 0 .022 0 .035 0 .023 0 .029 0 .019 0.034 0.038 0.050 0.029 0.038 0_050 0.033 0.034 0.038 0 .054 0 .047 LOD =limit of detection (0 .0003 KmoUkg) LOQ =limit of quantitation (0.0043 EunoUlcg) Q,OD <LOD <LOQ <LOQ Q.OD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ _ <LOQ <LOQ <LOD <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ 0.005 0.006 0.007 0.010 0 .010 0 .008 0 .014 0.019 0.020 0.024 0.017 <LOQ <LOQ <LOQ Q,OD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 239 - p. 240 Project No .: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFNA (jumol/kg) in Soils Treated with L Soil Type Sample Interval Aerobic Live Sterile Inceotisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 . Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.044 0.054 0.062 0.072 0 .079 0 .076 0 .134 0 .138 0 .128 0 .133 0.146 0_172 0.172 0_171 0 .154 0 .152 0 .159 0 .160 0 .171 0 .174 0.208 0.224 LOD =limit of detection (0.0003 WmoUkg) LOQ =limit of quantitation (0.0043 pmoUkg) <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ Q,OQ Anaerobic Live Sterile <LOQ <LOQ 0 .021 0 .016 0 .028 0 .029 0 .075 0 .047 0 .053 0 .057 0 .062 0.077 0.073 0.078 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ Q.OQ Friday, oawbQ 12, 2 oo7 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. ~ - 240 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PPDA (p.mol/kg) in Soils Treated with I Soi1 Type Sample Interval Aerobic Live Sterile Uttisol Day 0 Week 1 Week 2 Weok 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0 .009 0 .009 0 .015 0 .016 0 .021 0 .023 0 .035 0 .036 0.052 0.055 0.059 0.069 0.074 0.076 0.018 0.041 0.065 0.066 0.093 0.071 0.099 0.086 <LOQ <LOQ <LOQ <I,OQ dAQ <LOQ <LOQ <LOQ <LOQ Q,0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =limit of detection (0 .0002 Nmol/kg) LOQ =limit of quantttation (0 .0039 kmoUkg) Anaerobic Live Sterile <LOQ <LOQ 0.009 0.010 0.013 0.014 0 .018 0 .016 0 .026 0 .029 0 .049 0 .055 0 .043 0 .038 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ ' Y'duraday, November 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 241 - p. 242 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (wmol/kg) in Soils Treated withc Soil Type Sample Ixterval Aerobic Live Sterile Mollisol Day 0 W=k 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.007 0.007 0.009 0.010 0_012 0.012 0 .019 0 .020 0 .028 0 .032 0 .034 0 .034 0 .032 0.024 0.038 0.036 0,028 0.054 0.043 0.042 0.088 0 .094 LOD =limit of detection (0 .0002 wmoUkg) LOQ =1imit of quantitation (0.0039 IxmoUkg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.004 <LOQ <LOQ <LOQ <LOQ 0 .004 <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ 0.007 0.007 0.010 0.009 0.013 0.013 0.021 0 .021 0 .034 0 .028 0 .041 0 .034 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 7h wsday, xoyembu oe. 2W7 Company Sanitized . Does not contain TSCA CBI . Wildlife InteYnational, Ltd. - 242 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (M,mot/kg) in Soils Treated witl~ Soil Type Sample interval Aerobic Live Sterile Alfisol Day 0 Week i We& 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 MoirtL 15 Month 18 Month 24 <LOQ <LOQ Q,OQ 0 .004 0 .006 0 .006 0 .009 0.009 0.016 0.018 0.016 0,019 0_018 0_024 0 .033 0 .038 0 .025 0.031 0.051 0.030 0.045 0 .040 0.073 0 .071 LOD =limit of detection (0 .0002 pmoUkg) LOQ= limit of quantitation (0 .0039 umol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ 0.005 0 .006 0 .007 0 .011 0 .011 0 .009 0 .017 0.025 0.026 0.028 0.024 <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Frday, Ocfobn 12, 2007 Company Sanitized. Does not contain TSCA CBI . Wildlife International, Ltd. - 243 - Project No. : 112E-108 Wildlife International, Ltd. Project Number i 12E-i08 Measured Concentrations of PFDA (jLmollkg) in Soils Treated witbc Soil Type Sample Interval Aerobic Live Sterile Incentiso! Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LpQ <LOQ 0 .009 0 .010 0 .016 0 .016 0 .030 0.030 0.067 0.058 0 .085 0 .098 0 .102 0 .134 0.130 0.111 0.121 0.136 0.122 0.128 0 .158 0 .151 0 .169 0 .174 LOD =lunit of detection (0 .0002 WmolQcg) LOQ =1mmrt of quantita.tion (0 .0039 pmollkg) 4.,OQ <LOQ <LOQ <,OQ <LOQ <LOQ <LOQ <LOQ 0.004 Q,OQ 0.005 <LOQ 0 .006 0.006 0 .006 0 .005 0 .004 0.004 Anaerobic Live Sterik <LOQ Q..OQ 0.010 0.009 0.015 0 .015 0 .041 0 .022 0 .042 0 .045 0 .065 0.076 0.091 0.080 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0 .005 <LOQ Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 244 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA C=oUkg) in Soils Treated with( Soil Type Sample Interval Aerobic Live Sterile Uitisol DayO Week i Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Q,OQ <LOQ Q.OQ Q,OQ 0.005 0.006 0.009 0.008 0.015 0.014 0.023 0.027 0.028 0 .029 0 .033 0 .030 0 .006 0 .019 0 .027 0 .028 0 .034 0 .029 0.040 0 .040 LOD =limit ofdetection (0.0002 FunoVkg) LOQ =lmiit of quantitation (0 .0036 jtmoUkg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ 0 .005 0.005 o.007 0.007 0 .012 0 .015 0 .027 0 .023 0.032 0 .031 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, Odober 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 245 - p. 246 Project No .: 112E-108 Wildlife International, Ltd. ProjectNumber 112E-108 Measured Concentrations of PFUA (~mollkg) in Soils Treated wittc Soil Type Sample Interval Aerobic Live Sterila Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Mouth 15 - Month 18 Month 24 <LOQ d.OQ <LOQ <LOQ <LOQ 0 .004 0 .005 0 .005 0 .011 0 .010 0 .018 0 .019 0.017 0.017 0.015 0.008 0 .016 0 .015 0 .011 0 .020 0 .018 0 .016 0 .026 0.038 LOD =limit of detection (0 .0002 Eunollkg) LOQ =lumut of quantiRation (0 .0036 pmoUkg) <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ Q,OD QAQ <LOQ <LOQ <LOQ <LOQ <LOQ Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q. .OQ 0.004 0.004 0 .006 0 .011 0 .008 0 .012 0.010 <LOQ <LOQ <LOQ <LOQ Q.OD Q,OD Q,OQ <LOQ Q.OD <LOD <LOQ <LOQ <LOQ <LOD Friday, Odober 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -246- Project No. : 112E-108 Wildlife International, Ltd. ProjectNurnber 112E-108 Measured Concentrations of PFUA (Itmol/kg) in Soils TreatedwithC ] Sarnple Soil Type - - Interval Aerobic Live Sterile Alfisol Day 0 Week l Week 2 weak 4 Month z Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOQ Q,OQ Q,OQ Q,OQ 0 .004 0 .004 0.008 0.011 0.007 0.011 0 .006 0_011 0.013 0.020 0.014 0.016 0.024 0.010 0.012 0.013 0.025 0.018 LOD =1unn of deteo6on (0 .0002 ,,unolvlcg) LOQ =limit of quantitation (0 .0036 jimol/kg) <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD Q.OQ <LOQ <LOD <LOQ Q,OQ Anaerobic Live Sterile <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <.OQ 0 .005 0 .005 0 .006 0 .008 0 .006 <LOD <LOD <LOD <LOD Q,OQ <LOQ <LOD <LOD d,oQ <LOD <LOD <LOD "<LOQ <LOQ Friday, October 12, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -247- p. 248 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA (jLmol/kg) in Soils Treated with[ soil ywe __ Sa-ple interval Aerobic Live sterile Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 1S Month 24 <LOQ Q,OQ <LOQ 0 .004 0 .007 0 .008 0 .013 0 .011 0 .034 0 .035 0 .033 0_033 0_037 0_050 0_052 0_050 0.046 0.052 0.046 0.045 0.062 0.058 0.072 0.080 <LOQ <LOQ Q.OQ Q.OQ Q,OD <LOD <LOD Q,OD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <.OQ QAQ <LOQ LOD = limit. of detection (0 .0002 Eunol/W LOQ = limit of quantitation (0 .0036 lunoUkg) Anaerobic Live Sterile <LOD <LOD Q,OQ <LOQ 0_006 0 .006 0_017 0.011 0 .025 0.025 0 .030 0 .034 0 .039 0 .037 <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ F.iaaD~ octob~ ia, 2907 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 248 - Project No. : 112E-108 Wildlzfeinternational, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (p.mol/kg) in Soils Treated wit< Soil Type Sample Interval Aerobic Live Sterile Ultisol Day 0 Week I W=k 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ < .OQ <LOQ Q.OQ <,OQ Q.OQ <LOQ <LOD <LOQ Q,OQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (0 :048 ! unoUkg) LOQ =limit of quantitation (0 .216 Funollkg) Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD " <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Monday, Oaober IS, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 249 - p. 250 Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (pmol/kg) in Soils Treated with[ Soil Type SanrTle Interval Aerobic Live Sterile Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0.048 lunol/kg) LOQ =limit of quantitation (0 .216 Eunol/kg) Anaerobic Live Sierile <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Monday, October rs, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 250 - Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (pmol/kg) in Soils Treated withc Soil Type Sample Interval Aerobic Live Sterile A18so1 Day 0 Week i Week 2 ' Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD Q.OD Q,OD <,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD _ LOD =limit of detection (0 .048 EunoUkg) LOQ =limit of quantitation (0 .216 Wnollkg) Anaerobic Live Sterile <LOD <LOD <.OD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Monday, October IS, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 251 - p. 252 Project No.: 112E-108 Wildlife International, Ltd. ProjectNumber 112E-108 Measured Concentrations of 8-2 FTOH (p.mol/kg) in Soils Treated withl Soil Type Sample Interval Aerobic Live Sterile Incentisol Day 0 Week 1 Week 2 Week 4 " Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD Q,OQ Q,OQ 0 .374 0 .269 0_319 0 .297 <LOQ 0 .302 0 .216 <1,OQ 0.282 0.356 0.374 0_261 <LOQ <LOQ <LOQ <LOQ LOD =lnnit of detection (0 .048 gmol/kg) LOQ =1unrt of quantitation (0 .216 Funo1/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 4.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD Anaerobic Live Sterile <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD Monday, October 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 252 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (wmol/L) in Headspaces Gases from Chambers Treated withC Soil Type Sample Interval Aerobic Live Sterile Ultisol Day 0 Week 1 Week2 Wedc 4 . Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <,OQ <,OQ <LOQ <LOQ <L,OQ <LOQ <LOQ <,0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 4 .0Q <LOQ <LOQ <.OQ 4,OQ <LOQ <LOQ <LOQ < .0Q <LOQ <LOQ <LOQ d,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =limit of quantitation (0 .027 Wmol/L of headspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,0Q <LOQ <LOQ Monday, October 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife InteYnational, Ltd. -253 - p. 254 Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations,of8-2 FTOH (pmol/L) in Headspaces Gases from Chambers Treated withC Soil Type - - - Sample Interval Aerobic Live Sterile Mollisol Day 0 Week 1 W=k 2 Week 4 Month 2 Month 4 Month 6 Month 9 Mouth 12 Mouth 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = hmit of quantitation (0.027 ;Lmol/L of headspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -254- Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTOH (p.mol/L) in Headspaces Gases from Chambers Treated withE Soil T)pe Sample interval Aerobic Live Sterile A18so1 Day 0 Week l W=k 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ <LOQ <,0Q <LOQ <LOQ d,OQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =linrit of qnantitation (0 .027 N.moUL of beadspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <L,OQ Q .OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ Q,OQ <LOQ Q,OQ Mond4y, October ls, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 255 - p. 256 Project No. : 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of S-2 FTOH (pmoUL) in Headspaces Gases from Chambers Treated withE Soil Type Sample . Interval Aerobic Live Sterile Incentisol Day 0 week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =limit of quantitation (0.027 pmol/L ofheadspace) Anaerobic Live Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <i.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 15, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 256 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (P,mol/kg) in Soils Treated with I Soil Type Sample Interval Aerobic Live Sterile Ultisol Day o Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <,OQ <,OQ <,OQ Q,OQ <LOQ 0 .321 0 .401 <LOQ <LOQ <,OQ <LOQ 0.279 <LOQ <LOQ <LOQ <,OQ LOQ = limitofquantitation (0 .24 umollkg) Anaerobic Live Sterile Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 257 - p. 258 Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (p,moUkg) in Soils Treated with Soil Type Sample Interval Aerobic Live Sterile Mollisol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month ls Month 24 <LOQ Q <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q .OQ <LOQ <LOQ <LOQ Q.OQ LOQ =limit of quantitation (0 .24 jiuwUicg)' Anaerobic Live Sterile Tfiirrsday, Nwember 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 258 - Project No. : 112E-108 .Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (p.moUkg) in Soils Treated with C Soil Type Sample Interval Aerobic Live Sterile Anaerobic Live Sterile A1fiso1 Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <L.OQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ LOQ =limit of quantitation (0 .24 Eunollkg) 27&~~aoy, NA.emb~ oa, zoo7 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 259 - p. 260 Project No .: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-2 s FTOHJ (ILmol/kg) in Soils Treated with[ Soil Type Sample Interval Aerobic Live Sterile Inceotisol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ Q.OQ <LOQ <LOQ 0 .672 0 .672 0 .466 1 .121 0.466 0.287 0.418 0.440 <LOQ <LOQ <LOQ <,OQ LOQ =litnit of qnantitarion (024 pmol/kg) Anaerobic Live Sterile 77eursday, Novernber 08, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -260- Project No.: 112E-108 Wildlife International, Ltd. Project Number l I2E-108 Measured Concentrations of 7-3 Acid (pmolJkg) in Soils Treated withE Soil Type Sample Interval Aerobic Live Sterile Ultisol Day o Month 2 Month 6 Month 9 Mouth 12 Month 15 Month 18 Month 24 Q.OQ <LOQ 0.015 0.021 0.037 0.048 0.071 0.065 0 .094 0 .076 0.077 0.091 0.063 0.038 0.123 0.127 LOQ =limit of quantitation (0 .0045 Vmol/kg) AnaerobicLive Sterile Wednesday, March 12, 2008 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 261 - p. 262 Project No .: 112E-108 -Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-3 Acid (pmoUkg) in Soils Treated with[ Soil Type Sample Interval Aerobic Live Sterile Mollisol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Q,OQ <LOQ <LOQ <LOQ 0.005 0.006 0.006 0.006 0.011 0.011 0.015 0 .020 0 .008 0 .008 0 .020 0 .015 LOQ =limit of quantitation (0.0045 limoUkg) Anaerobic Live Sterile we*baaQy Mayx* 12,2009 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No.: 112E-108 Wildlife International, Ltd. Project Number 112B-108 Measured Concentrations of 7-3 Acid (ILmol/kg) in Soils Treated withc Soil Type - Sample Interval Aerobic Live Sterile Alfi .yol Day 0 Month 2 Mouth 6 Month 9 Month 12 Month 75 Month 18 Month 24 <LOQ <LOQ QOQ <LOQ 0.007 0.006 0.007 0.006 0.011 0.014 0 .017 0 .020 0 .018 0 .015 0 .015 0 .016 LOQ=limit of quantitation (0 .00451emoUkg) Anaerobic Live Sterile Wednesday, March I2,1008 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 263 - p. 264 Project No.: 112E-108 Wildlife International, Ltd. Project Number ii2E-to8 Measured Concentrations of 7-3 Acid (pimoUkg) in Soils Treated witht Soil Type Sample Interval Aerobic Live Stertle Inceotiso3 Day 0 Mouth 2 Month 6 Month 9 Month 12 Month 15 Month 18 Monfli24 <LOQ Q,OQ 0.038 0.022 0.153 0.185 0 .290 0.347 0 .626 0 .557 0 .590 0 .655 0 .638 0 .480 0 .803 0 .728 LOQ = limit of quantitation (0 .0045 FunoUkg) Anaerobic Live Sterile Wednesday, March 12, 2008 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -264- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFOA (p.mol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic U11:isol , Day 0 Week 1 Week.2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =limit of detection (0.0005 tlmol/kg) LoQ = limit of quantitation (0.0048 Ellnovlcg) Fortified Recoveries Aerobic Anaerobic 0.023 0.019 0.021 0.022 0.022 0.022 0.025 0.025 0.029 0.028 0.026 0 .025 0 .022 0 .024 0.018 0 .017 95% 78% 86% 91% 97% 92% 104% 303% 120" 114% 106% 104% 92% 100% 7456 ss% 0.023 0.022 0.025 0.027 0.022 0 .024 0 .023 0 .022 0 .027 0 .026 0 .027 0 .027 0 .027 0 .028 95% 92% 105% 113% 9266 98% 93% 90% 114% 10895 114% 113% 112% 115% Friday, October 12, 2007 Page I of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -265- p. 266 Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFOA (Pmol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic MoRisol Day 0 week 1 week 2 week 4 Month 2 Month 4 Month 6 month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Q.OQ <LOD <LOD <LOQ <LOD <LOQ <LOQ 4.OQ <LOD <LOD <LOD <LOD <LOD <I,OQ <LOD <LOD <LOD Q.OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0 .0005 FlmoUko LOQ =limit of quantitation (0.0048 ilmoUkg) ForliftedRecoveries Aerobic Anaerobic 0.022 0.022 0.023 0.024 0.021 0 .023 0 .022 0 .022 0.026 0.027 0.032 0.029 0.032 0.029 0.028 0.028 0.022 0 .025 89% 93% 974'. 100% 88% 94% 91% 90% lone 112% 132% 121% 131% 120% 114% 116% 92% 104% 0.023 0.024 17.020 0.023 0.020 0. 021 0.023 0.024 0.030 0.026 0.034 0.034 0.035 0.035 93% lol% 85% 96% 85% 89% 95% 99% 122% 109% 140% 139% 145% 143% Friday, October 12, 2007 pw 2 0! 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -266- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFOA (p,mol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week I Week 2 R'4=k 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <,oD <LOD <LOD <LOQ <LOQ <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD QAD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <,OD <LOD <LOD <LOD LOD = limit of detection (0.0005 Wmol/kg) LOQ =limit of quantitation (0.0048 Eunol/kg) Fortified Recoveries Aerobic Anaerobic 0 .022 0 .020 0 .022 0 .024 0_024 0.023 0.024 0.024 0_026 0 .026 0 .026 0_028 0 .027 0 .027 0.028 0.030 0.022 0.022 92% 83% 99% 100% 99% 9a% ioo% ioi% iiax 107% 108% us% 110% iusc 115% izs% 92% 93% 0_022 0.020 0.021 0.022 0.023 0 .024 0 .025 0 .025 0.025 0.026 0.027 0.026 0.025 0.027 92% 82% 97% 93% 9694 ioosa 106% 104% 104% io7sc iii% 105% 112% Friday, october 12, 2007 Page 3 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -267- p. 268 Project No . : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFOA (pmol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Inceotisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 month 9 lvSoath 12 Month 15 Month ls Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <,OQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <I,OQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <,OQ LOD =1k& of detection (0.0005 pnwUkg) LOQ =limit of quantitation (0.0048 ilmol/kg) Forfifled Recoveries Aerobic Anaerobic 0 .025 0.025 0.031 0.024 0.026 0.026 0.030' 0.027 0.027 0 .028 0 .032 0.026 0.029 0.026 0.035 0.030 0.023 0 .023 104% 105% 129% 101% 306% 109% 124% 112% 113% 117% 134% los% 121% 106% 145% 124% 96% 96% 0.023 97% 0.023 94% 0.026 309% 0.024 98% 0.026 loa% 0.025 105% 0.026 107% 0.026 109% 0.029 119% 0.027 111% 0.026 106% 0.028 115% 0.027 113% 0.028 l ls% Friday, October I2, 2007 Page 4 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 268 - Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTLJA (jitmol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated ControLs Aerobic Anaerobic Ultisol Day 0 week 1 Week 2 Week 4 Month 2 Month ,t Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Q,OQ <.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0 .0003 IunolQcg) LOQ =limit of quantitation (0.0044 Itmol/kg) Fort:'feed Recoveries Aerobic Anaerobic 0 .016 72% 0 .014 65% 0 .017 r7% 0 .017 0.015 69% 0.015 69% 0 .016 72% 0 .016 0 .019 a% 0.017 80% 0.014 65% 0.016 n% 0.006 29% 0.012 54% 0.011 51% 0.009 ao% 0.017 ao% 0.016 74% 0_017 77% 0.019 97% 0.018 94% 0.018 sz% 0.018 82% 0.017 79% 0.020 93% 0.017 76% 0.018 84% 0.017 80% 0.014 65% 0.015 70% Friday, October 12, 2007 Pags 1 0f4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -269- p. 270 Proj ect No.: 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Coneentrations of 8-2 FTUA (ja.mol/kg) in Untreated and Fortified Soils Soil Tyre Sample Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 Week 1 Wwx 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD QDD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD a.OD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD Q .OD <LOD LOD = limit of detoction (0 .0003 Winollkg) LOQ = limitof quantitation (0 .0044 Pmollkg) Fortified Recoveries Aerobic Anaerobic 0.020 0.020 0 .018 0 .019 0 .020 0 .021 0 .020 0.020 0.019 0.019 0.022 0 .020 0 .022 0 .018 0 .017 0 .020 0.013 0.014 92% 90% 91% 85% 91% 95% 9o+c 91% B8% 97% 100% 9a% l02% az% 79% 90% s9% 63% 0.016 73% 0.019 ss% 0.017 M 0.019 sr% 0.017 77% 0.021 98% 0.018 so% 0.018 so% 0.021 97% 0.017 78% 0.022 9994 0.023 lo% 0.022 loo% 0.019 ss% FrFday, Orlobcr 12, 2007 Page2 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -270- Project No .: 112E-108 Wildlife International, Ltd. Project Numbers 1121r108,109,110 Measured Concentrations of 8-2 FTUA (p,mol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 . Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =liumit of detection (0.0003 wmol/kg) LOQ = limit of quandtation (0.0044 pmol/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortffied Recoveries Aerobic Anaerobic 0 .018 0.017 0.019 0.021 0.023 0 .017 0.016 0.020 0.021 0 .020 0.014 0 .020 0 .018 0 .020 0.018 0 .024 0 .014 0 .015 s4% 77% 86% 98% 105% 76% 75% 92% 96% 91% 66% 9a% s4% 90% 90% 111% 62% 67% 0 .020 0 .016 0 .020 0.021 0_020 0_019 0 .019 0 .020 0.024 0.024 0.018 0.018 0 .023 0 .018 89% 75% 93% 95% 92% 89% 96% 94% 109% 108% 81% 93% 103% 83% Friday, October 12,1007 Page 3 aj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 271 - p. 272 Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (pmol/kg) in Untreated and Fortified Soils Soil Type- Sarnple Interval Untreated Controls Aerobic Anaerobic Incentisol Day 0 Weak 1 Week 2 Week 4 Month 2 Mamth 4 Month 6 Month 9 Month 12 month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0 .0003 lunoAg) LOQ =limit of quantitation (0 .004411moUkg) Fortl;fied Recoveries Aerobic Anaerobic 0.024 0.021 0.024 0.018 0.019 0.021 0.020 0.017 0.018 0.020 0.022 0.018 0.015 0.015 0.016 0.014 0.012 0.011 110% 96% 109% 93% 8856 97% 94% 79% eo% 89% 101% 81% 70% 68% 74% 641A 53% 5278 0.017 79% 0.018 81% 0.018 93% 0.016 75% 0.019 85% 0_017 78% 0.017 77% 0.018 82% 0.017 76% 0.019 s89A 0.014 66% 0.015 70% 0.013 61% 0.016 71% Friday, October 12, 2007 Page 4 off Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 272 - p. 273 Project No.: 112E-108 Wildlife International, ,Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (p.mol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Forh;fied Recoveries Aerobic Anaerobic Uttisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 . Mouth 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Q,OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 0.013 61% 0.012 58% 0.013 62% 0.014 sv% 0.011 54% 0.011 55% 0.011 54% 0.011 53% 0.007 33% 0.005 za% 0.007 34% 0.008 37% <LOQ <LOQ 0.009 41% <LOQ 0 .014 0.014 0.015 0.015 0.013 0.014 0 .011 0 .013 0.015 0.011 0.010 0.010 0.007 0 .007 67% 66% 70% 69% 61% 65% 53% 63% 72% 51% 48% 46% 35% 32% LOD = limit ofdetection (0.0011 p.mollkg) LOQ =lilnit of quantit'at%on (0 .0042 umolllkg) Friday, Odobo 11,1007 Page 1 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -273- p. 274 Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (p,mol/kg) in Untreated and Fortified Soils Soil Type - Sample Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 Week 1 Week 2 Wxk4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD 0 .006 <LOD <LOD <LOD <LOQ <LOD <LOD Q.OD <LOD <,OD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD LOD = limit of detection (0 .0011 lxmollkg) LOQ =limit of yuantitation (0 .00421unoUkg) Forh'fied Recoveries Aerobic Anaerobic 0.018 97% 0.019 90% 0.018 u% 0 .019 90% 0 .019 90% 0.018 98% 0.017 83% 0.017 si% 0.016 78% 0.017 79% 0.017 94% 0.014 69% 0.013 64% 0.013 62% 0.010 50% 0.013 62% 0.005 23% <LOQ 0.015 70% 0.018 s7% 0.015 71% 0.016 76% 0.013 64% 0.017 s3% 0.013 63% 0.015 70% 0.017 so% 0.015 71% 0.012 58% 0.014 65% 0.012 57% 0.011 52% Friday, October 12, 2007 Page 2 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -274- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA ()LmoUkg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 week 2 Week 4 Wnth 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <,OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q .OD Q.OD <LOQ <LOQ LoD = limit of detection (0.0011 gmlvlg) LOQ =limit of quantitation (0 .0042 funol/kg) Forttfied Recoveries Aerobic Anaerobic 0 .016 0 .017 0.016 0.018 0.022 0.018 0.014 0 .017 0 .016 0.015 0.009 0 .014 0 .013 0 .014 0.011 0.016 0.008 0 .008 79% 79% 79% 84% 106% sna 62% 94% 75% 70% 42% 67% 60% 66% 51% rn% 40% 37% 0.018 SS% 0_014 67% 0.018 ss% 0.018 94% 0.018 84% 0.017 81% 0.016 77% 0_017 93% 0.018 asec 0.017 83% 0.014 67% 0.014 66% 0 .015 73% 0 .015 70% Friday, Odober 12, 2007 Page 3 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -275- -- : :~ p. 276 Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (p.mol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Inceotisol Day 0 Week 1 Wcek 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <.OD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD d,OD <LOD <,OD <LOD <LOQ Q.OQ <LOQ <LOD <LOD <LOD <LOQ LOD =lunit of detection (0 .0011 lemol/kg) LOQ =limit of quantitation (0 .0042 EunaUkg) Q.OD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic Anaerobic 0.022 0.020 0.020 0.016 0.015 0.018 0 .015 0 .013 0 .011 0 .012 0 .011 0 .009 0.009 0.008 0.007 0 .007 0 .004 <LOQ 107% 96% 98% 76% 70% 94% 71% 63% 55% 58% 53% 45% 43% 37% 34% 34% zi% 0.016 78% 0.015 72% 0.015 70% 0.014 67% 0.015 72% 0.016 76% 0.012 59% 0.014 66% 0.010 49% 0.013 63% 0.008 36% 0.007 34% 0.005 26% 0.007 35% Friday, October I2, 3007 Page 4 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 276 - p. 277 Project No.: 112E-108 Wildlifc International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (ALmollkg) in Untreated and Fortified Soils Soil Type _ _ Sanrple Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Q,OQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ, <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD =limit of detection (0.00031unol/kg) LOQ =limit of quantitation (0 .0043 llmollkg) Forhfied Recaverfes Aerobic Anaerobic 0.020 o.017 0 .021 0 .021 0 .021 0 .021 0 .022 0 .022 0 .024 0 .024 0 .022 0 .023 0.015 0.022 0 .017 0 .014 94% 78% 97% 98% 95% 98% 104% lol% 114% 114% 103% 106% 70% 103% 79% 67% 0 .022 0 .021 0 .021 0 .023 0 .021 0 .022 0 .022 0 .021 0 .024 0 .024 0.025 0.025 0.023 0.025 100% 99% 99% 307% 97% 303% 103% 96% 112% luo% 116% 117% los% 116% Friday, Oetober I2, 2007 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -277- p. 278 Project No.: 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of PFNA (AmoUkg) in Untreated and Fortified Soils Soil Type Sanrpie Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 week 1 Week 2 week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD Q.,OD Q,OD <LOD <LOD <LOD <LOD Q.OD <LOD <LOD LOD =limit of dotection (0 .0003 lllnol/kg) LOQ =limit of quantitation (0.0043 11moUkg) Forh;fied Recoveries Aerobic Anaerobic 0 .020 0 .021 0 .021 0 .021 0 .021 0 .021 0 .022 0 .018 0 .023 0.023 0.026 0.026 0.026 0.025 0.022 0.024 0.017 0 .018 94% 96% 97% 97% 93% 99% 102% 96% lorx 106% 122% 119% 120% In% 100% 112% 81% 83% 0 .021 0 .022 0 .019 U .021 0 .021 0 .021 0 .020 0 .021 0 .023 0 .022 0.025 0 .028 0 .026 0 .026 96% 101% 89% 98% 96% 99% 93% 98% 107% lol% 116% 128'X 122% 119% Friday, October 12, 2097 Page 2 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -278- Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (p.moi/kg) in Untreated and Fortified Soils Sample Soil Type -- Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 Wwx 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD Q,OD Q.OD <LOD <LOD Q,OQ <LOQ <I,OQ Q,OQ <LOQ Q,OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ LOD =limit of detection (0_0003 pmol/kg) LOQ =limit of quantitation (0 .0043 Elmol/kg) Fortified Recoveries Aerobic Anaerobic 0 .021 0.020 0.020 0.024 0 .022 0_020 0 .022 0.023 0.024 0.024 0.022 0.025 0.023 0.025 0_024 0.026 0.019 0.018 98% 93% 94% 112% 303% 91% 103% 107% 113% Llo% loo% 114% loa% 114% 113% 323% 87% 96% 0.021 0.017 0 .020 0 .022 0.021 0.022 0.022 0.022 0.024 0_024 0.023 0_023 0.025 0.022 97% 8176 94% 101% 98% 301% 103% 100% 110% no% 107% 107% 115% 102% Friday, October 12, 2007 Pap 3 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 279 - p. 280 Project No. : 112E-108 Wildlife International, Ltd. , Project Numbers 112E-108,109,110 Measured Concentrations of PFNA (p.moUkg) in Untreated and Fortified Soils Soil Type- Sampla Interval Untreated Controls Aerobic Anaerobic Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ Q,OQ <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <_.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <L0Q <LOD LOD =limit of detection (0 .0003 l,moUkg) LOQ =limit of quantitation (0 .0043 lunol/kg) <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ FortifiedRecoveries Aerobic Anaerobic 0.027 0.025 0.028 0.023 0.023 0.023 0.027 0.023 0.024 0.026 0.030 0.023 0.022 0.021 0 .028 0.023 0.019 0 .019 124% 116% 130% 106% 107% 107% 125% 107% 11276 119% 140% los% 104% 100% 130% 109% 99% 88% 0 .022 0 .022 0 .023 0 .021 0 .022 0.022 0.022 0.023 0.024 0.024 0.024 0.023 0.022 0 .023 102% 100% 108% 98% 103% 102% 101% 107% 111% 111% 110% 109% 101% 108% Friday, October 12, 2007 Page 4 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -280- Project No . : 112E-108 Wildlife International, Ltd. Project Numbers 1 12E- 108,109,110 Measured Concentrations of PFDA (p.moUkg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 19 Month 24 <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ LOD = limit of detection (0 .0002 Funollkg) LOQ =limit of quantitation (0 .0039 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ < .,0Q <LOQ <LOQ Fortc;fied Recoveries Aerobic Anaerobic 0.018 93% 0.014 74% 0.017 e9% 0.017 86% 0.017 88% 0.017 e9% 0.018 94% 0.018 93% 0.021 109% 0.021 109% 0 .019 96% 0.019 100% 0 .010 52% 0.018 95% 0.016 84% 0.014 70% 0.018 92% 0.018 91% 0.018 90% 0.019 97% 0.018 94% 0.018 92% 0.018 93% 0.016 90% 0_020 104% 0.020 102% 0.021 110% 0_021 109% 0 .019 96% 0.020 304% Friday, October 12, 2007 Page Z of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. -281 - p. 282 Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 1128-108,109,110 Measured Concentrations of PFDA (p.mol/kg) in Untreated and Fortified Soils Soil Type - Sample interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 Weak 1 Week 2 Week 4 Month 2 Mouth 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ Q,OQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD Q.OD <LOD <LOD <,OD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <IAQ Q.OQ 4,OQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0 .000211a1ol/kg) LOQ =lirnit of quantitation (0 .0039 Nmolllcg) Fortifted Recoveries Aerobic Anaerobic 0 .019 0 .019 0.019 0.020 0.019 0.020 0.019 0.016 0.019 0 .019 0 .023 0 .021 0.024 0.022 0.018 0.020 0.015 0.016 100% lo0% 9856 104% 97% 101% 98% 81% 99% 97% ln% 109% 121% 112% 92% 102% 76% am 0 .018 0 .019 0 .016 0 .018 0 .018 0 .018 0 .017 0 .018 0 .020 0 .018 0 .022 0.024 0.022 0 .021 92% 99% 82% 95% 92% 93% 90% 90% 104% 92% 114% 121% 112% 109% Friday, October 12, 2007 - Page 2 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 282 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFDA (}cmoUkg) in Untreated and Fortified Soils ' Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <I,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOQ <LOD <L.OD <LOD <LOD LOD =1imrt of detection (0.0002 Wno1/icg) LOQ = limit of quantitation (0 .0039 punollkg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <i .OD Q.OQ Fortlfed Recoveries Aerobic Anaerobic 0 .018 0 .018 0 .018 0 .021 0 .020 0.017 0.018 0.020 0_023 0.022 0.018 0.021 0 .021 0 .022 0.021 0 .026 0 .018 0 .018 93% 93% 94% Ilo% 101% 96% 90% 105% 119% 112% 93% 109% 107% 112% 309% 133% 92% 93% 0.018 0 .014 0 .017 0 .018 0 .018 0 .019 0 .019 0 .019 0 .022 0 .022 0.018 0.019 0.023 0.017 92% 74% 97% 93% 90% 95% 97% 96% 113% 114% 90% 9s% 116% 96% Friday, Odobv 12, 2007 Page 3 Jr4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 283 - p. 284 Project No. : 112E-108 Wildlzfe International, Ltd. Project Numbers 1121<108,109,110 Measured Concentrations of PFDA (p.mol/kg) in Untreated and Fortified Soils Soil Type Sample -- - Interval Untreated Controls Aerobic Anaerobic Incentisol Day 0 Wcek 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <,OQ <LOQ Q.OQ <LOQ Q .OQ <LOQ <LOQ <LOQ QOQ <LOQ <LOQ <J,OD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD = linzit of detection (0 .0002 tllnoUkg) LOQ =limit ofquantitation (0 .003911mol/kg) Fortified Recoveries Aerobic Anaerobic 0 .027 0 .024 0 .028 0 .023 0 .022 0 .022 0.024 0 .022 0.023 0.026 0 .027 0.021 0 .021 0.018 0.025 0.021 0.018 0 .018 139% 124% 146% 116% 113% 116% 121% lls% 120% 133% 141% los% 107% 92% 128" lU9% 93% 92% 0.019 0.019 0.021 0 .019 0 .021 0 .020 0 .020 0 .021 0.023 0.024 0 .021 0 .023 0 .019 0.021 99% 97% 107% 96% 108% 103% 101% 107% lla% 122% 108% 116% 96% 106% Friday, Odobe 1Z, 2007 Page 4 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 284 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (WmoUkg) in Untreated and Fortified Soils Soil Type- -- Samp(e Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week 1 Wak 2 Week 4 Month 2 Month 4 Month 6 Month 9 ~ Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <..OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <,OD <LOQ <LOQ <L,OQ <LOQ <LOQ <LOQ Q.OD <LOQ LOD =limit of detection (0.000211mo1/kg) LOQ =limit of quantitation (0 .003611mol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Fortified Recoveries Aerobic Anaerobic 0.015 0 .012 0.015 0.014 0.013 0 .014 0 .015 0 .013 0 .018 0.018 0.016 0.017 0 .007 0 .015 0 .013 0.011 95% 69% 92% 82% 76% 78% 86% 74% 102% 101% 92% 93% 41% 84% 72-A 62% 0.015 85% 0.016 88% 0.015 85% 0.015 84% 0.016 89% 0.015 86s 0.016 91% 0.012 70% 0.017 99% 0.017 95% 0.017 94% 0.017 95% 0.016 99% 0_018 99% Friday, Oaober 11, 2007 Page 1 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 285 - p. 286 Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (p.mol/kg) in Untreated and Fortified Soils Soil Type Sanple Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OQ Q.OQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD LOD =limit of detection (0.00021ccnol/kg) LOQ =limit of .quantitation (0.0036 prnol/kg) <LOQ <LOQ <LOQ <LOD <LOD Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic Anaerobic 0.017 0.017 0 .015 0 .017 0 .016 0 .016 0 .015 0.012 0.015 0 .015 0 .018 0 .018 0.017 0.015 0.013 0 .016 0 .011 0.011 95% 96% 97% 93% 90% 92% 87% 69% 94% 94% lolx lol% 95% e7-A 74% ss% 61% 63% 0.015 82% 0.015 86% 0.012 67% 0.014 76% 0.011 63% 0.013 71% 0.011 63% 0.014 80% 0.015 83% 0.013 75% 0.017 96% 0.017 94% 0.017 98% 0.014 79% . Friday, Octobo 12, 2007 Page 2 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 286 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (p.moUkg) in Untreated and Fortified Soils Soil Type - SarnPle Interval Untreated Controls Aerobic Anaerobic ,^,lfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OQ Q,OQ Q.OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOQ <LOQ LOD =liunit of detection (0 .0002 ~lmoUkg) LOQ =limit of quantitation (0 .0036 tunol/kg) Fortified Recoveries Aerobic Anaerobic 0 .016 92% 0 .016 s7% 0 .016 0 .019 92% 109% 0 .017 94% 0 .014 0 .014 0 .017 0 .018 0 .017 0 .013 80% 77% 94% 103% 97% 75% 0 .016 E9% 0 .016 89% 0 .016 93% 0.015 96% 0.022 122% 0.016 97% 0.016 ss% 0.015 83% 0.011 61% 0.015 83% 0.015 86% 0.014 79% 0.014 79% 0.012 70% 0.013 76% 0.016 as% 0.017 99% 0.013 74% 0.013 72% 0_017 95% 0.012 67% Friday, Odober 12,10D7 Page 3 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 287 - p. 288 Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of PFUA (p.mol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Incentisol Day 0 Weak 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <I,OQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ LOD = find of detection (0.0002 pmollkg) LOQ = limit of quantitation (0 .0036 pmollkg) ForgfledRecoveies Aerobic Anaerobic 0 .021 0 .020 0 .022 0 .018 0 .016 0 .018 0 .017 0 .016 0 .018 0 .021 0.022 0.016 0_014 0 .015 0 .018 0 .015 0 .013 0 .012 120% 112% 125% 101% 92% 103% 98% 89% lati% 117% izu% 88% az% 83% 99% 82% 74% 69% 0 .014 0 .013 0 .014 0 .013 0 .013 0 .015 0.014 0_015 0.015 0.018 0.012 0.013 0.012 0.015 79% 74% 79% 74% 74'h 87% 80% 85% 87% 103% 69% 73% 70% 95% Friday, October 12, 2009 Page 4 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 288 - p. 289 Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (M.moUkg) in Untreated and Fortified Soils Soil Type Sarnpte Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit ofdetection (0 .048 Ilmol/kg) LOQ =limit ofquantitation (0 .216 Ilmol/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD ' <LOD <LOD Foricfied Recoveries Aerobic Anaerobic 0.622 0 .609 0 .525 0.531 0_452 0.448 0.356 0.317 0_220 <LOQ <LOQ Q,OQ <LOQ <LOQ Q.OQ <L.OQ <LOQ Q.OQ 115% 113% 97% 99% 94% 83% 66% 59% 41% 39% 18^.5 21% 16% 9% 9% 0% 0% 0.627 116% 0.621 115% 0.475 se% 0.555 103% 0.536 99% 0.390 72% 0.385 71% 0_334 62% 0.356 66% 0.253 47es Q.OQ 35% <LOQ 229a <LOQ 27% Monday, October 15, 2007 Page 1 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. ~- 289 - Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (p.mol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic MoDisol Day 0 weak 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit of detection (0 .048 pmol/kg) LOQ = limit of quantitation (0 .216 jimollkg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic Anaerobic 0.645 120x 0.562 104% 0.459 85''6 0.469 M 0.419 7r/* 0.241 45% 0.256 4M <LOQ zlx <LOQ zs% <LOQ ?% <LOQ 13% <LOQ 3% <LOQ 4% <LOQ o% <LOQ 3% <LOQ <LOQ 0.574 0.602 112% 0.526 0.589 0.554 103% 0.524 0.412 0.413 :IOQ 40% 0 .349 63% <LOQ <LOQ IVA <LOQ 14% q,OQ 6% - Monday, October 75, 2007 Page 2 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 290 - Project No. : 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (p.mol/kg) in Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Alfisol , Day 0 Week i Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q . .OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD Q.OD <LOD 4.OD <LOD LOD = lunit of detection (0 .048 pmoUkg) LOQ =1unt of quantitation (0 .216 Eunol/kg) <LOD Q,OD Q.OD <LOD <LOD Q.OD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD Fortified Recoveries Aerobic Anaerobic 0.662 0.527 0.453 0_309 0.336 0.307 0.252 0.241 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 123% 98% 84% 57% 62% M 47% 45% 30% 32% 22% 21% 5% 12% 4% a% 03< 0.574 0.548 0.462 0.437 0.441 0.347 0 .274 0 .308 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ iors 102% 86% si% 82% 64% 51% 57% 33% 25% 20% 15% 4% 7% Monday, October 15, 2007 1-age 3 of9 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 291 - p. 292 Project No. : 112E-108 WildlfeInternational, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH ()LmoUkg) in Untreated and Fortified Soils Soil Type Sarnple Interval Untreated Conirols Aerobic Anaerobic Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD Q,OD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =lilnrt of detection (0.048 pmoUkg) LOQ =limit of quantitation (0 .216 ;imollkg) Fortified Recoveries Aerobic Anaerobic 0.613 114% 0.565 105% 0.575 107% 0.613 114% 0.529 98% 0.505 94% 0.470 sM 0.612 114% 0 .434 sl% 0 .430 so% 0 .338 63% 0 .401 74% <LOQ 34% <LOQ 34% <LOQ 22% 0.248 46% <LOQ 0% <LOQ 0% 0.619 115% 0.613 114% 0.555 103% 0.560 104% 0.511 95% 0.507 94% 0.495 92% 0.471 97% 0.289 54% 0.356 66% 0.228 42% 0.228 42% <LpQ 17% <LOQ 15% Monday, October 15, 2007 rage 4 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 292 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers I 12E- 108,109,110 Measured Concentrations of 8-2 FTOH (p.mol/L) in Headspace Gases from Untreated and Fortified Soils Soil Type Sample Interval Untreated Controls Aerobic Anaerobic Ultisol Day 0 Week 1 Week 2 Week a Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ 4,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (0 .027 gmol/L ofheadspace) Fortified Soils Aerobic Anaerobic Q,OQ <LOQ <LOQ <LOQ <LOQ < .01Q Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,oQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q.,OQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Pagr I oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. p. 294 Project No.: 112E-108 -293......... Wildlife International, Ltd. ProjeccNumters ii2E-i 0s,109,ii0 Measured Concentrations of 8-2 FTOH (pmol/L) in Headspace Gases from Untreated and Fortified Soils _ Soil Type - Sample Interval Untreated Controls Aerobic Anaerobic Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (0 .027 pmoUL ofheadspace) Fortified Soils Aerobic Anaerobic <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ <LOQ <L.OQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q-OQ Monday, October 15, 2007 PAW 2 of4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 294 - Proj ect No.: 112E-108 Wildlife International, Ltd. ProjectNumbers 112E-10s,109,110 Measured Concentrations of 8-2 FTOH (pmoUL) in Headspace Gases from Untreated and Fortified Soils Soil Type s-Pre Interval Untreated Controls Aerobic Anaerobic Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ Q.OQ <LOQ Q.OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ , Q,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ <LOQ <LOQ ' = limit of quantrtation (0 .027 lemol/L of headspace) Forti,~xed Soils Aerobic Anaerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Q,OQ . Monday, October 15, 2007 Page 3 oj4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 295 - Project No. : 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,710 Measured Concentrations of &2 FTOH (p .mol/L) in Headspace Gases from Untreated and Fortified Soils Soil Type - Sarxple interval Untreated Controls Aerobic Anaerobic IncentisoF Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Mouth 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <.OQ <LOQ LOQ =1imrt of quantitation (0 .02'7 Kmol/L of headspace) Fortified Soils Aerobic Anaerobic a,oQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ QOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, Odobet IS, 2007 Page 4 of 4 Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Project No. : 112E-108 -296- Appendix lII Example Calculations A. Standard Curves by Linear Regression Standard curves were prepared by plotting the ratio of analyte concentration (~tg/L = ng/mL) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid to the internal standard concentration (0 .75 ng/mL 2-13C PFOA for 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA and 7-3 Acid and 100 ng/mL HDFD for 8-2 FTOH and 7-2 sFTOH) in solutions with known concentrations on the abscissa and the respective peak area responses on the ordinate . Weighted regression (1/x) was applied to analysis of the data to determine the equation with respect to the abscissa as shown below: A = [m x (C /Ci ) + b] x Ai where A Ai m C Ci b analyte area internal standard area slope analyte concentration (ng/mL) internal standard concentration (ng/mL) intercept Concentrations of analytes in the final solutions of processed samples, just prior to injection, were calculated using a rearrangement ofthe above equation: C = C' x (A/A;) - b m B. Residue Concentrations of 8-2 FTOH 8-2 FTA 8-2 FTUA PFOA PFNA PFD PFUA. 7-2 sFTOH and 7-3 Acid in Soil Extracts Residue concentrations (~ig/kg) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid in soil extracts were determined using the following equation : Sample Concentration in gg/kg = (C) ~V`) x MI) x (V2) (W) x (Vil) x (Vu) where C V.- Vii V,2 Vr, VQ W Concentration (ng/rnL) as determined above Extraction volume (125 rnL) . First initial volume (mL) Second initial volume (if required) First final volume (mL) Second final volume (mL) Weight ofsample (25 g dry weight) . Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. - 297 - p. 298 Project No. : 112E-108 Residue concentrations (~Lmol/kg) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid in soil extracts were determined using the following equation: Sample Concentration in ~Lmol/kg = C x molecular weight (~tmol/~Lg) where C = Sample concentration (~Lg/kg) as determined above C. Proce dural Recoveries in Fortified Controls Procedural recoveries were calculated for fortified control samples prepared in each test condition at known concentrations in 8-2 FTOH (250 gg/kg), 8-2 FTA (10.00 pg/kg), 8-2 FTUA (10 .00 pg/kg), PFOA (10 .00 gg/kg), PFNA (10 .00 ~tg/kg), PFDA (10 .00 gg/kg) and PFUA (10 .00 gg/kg). Percent recoveries of the each analyte were determined to provide confirmation that the method was performing adequately. Percent recoveries were determined by dividing the concentration of the analyte recovered in the fortified sample by the nominal concentration added as shown below: gg/kg Found o Percent Recovery = x 100 /o p.g/kg Added Company Sanitized . Does not contain TSCA CBI . Wildlife International, Ltd. Sampling Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Alfisol 0 7 14 . 28 63 119 182 273 365 462 546 728 -298Appendix IV Duration of Incubation Actual Days of Incubation Inceptisol 0 7 14 28 63 119 182 273 365 462 546 728 Ultisol 0 7 14 28 56 119 182 273 364 462 553 728 Project No. : 112E-108 Mollisol 0 7 14 28 56 119 182 273 364 462 553 728 Comp ~",, San ~tized