Document 06beQa9N3NbDMR0ov7zKy389b
AR 26 -- 085%
MICROBICS MICROTOX TOXICITY TEST
IESTSUBSTAN 000C 000E 000
Identity: A mixture containing perfiuorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number is not noted. The test sample is
FC-603EF. Current information indicates it is a mixture of 1.30% PFOS, 10%
diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6%
Cphrioopraonaelsluyllt)ohneexafmoianmieurm,
5% Alkylether chloride, 0.9%
sulfate, 0.1% N-(3Xanthan gum, 0.9%
starch,
1.0%
triethanolamine, and 0.05% tolyl triazole.
The following summary applies a mixture with incompletely characterized concentrationsof impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample.
METHOD:
00000000
Method: Microbics Microtox "BASIC" Procedure Test type: Acute static YGeLaPr:CoNmopleted: 1996 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions:
Diluent: Milipore Mil-QTM water Test temperature: Not noted. Stock and test solution preparation: A 500 mg/L primary stock MsiollluitpioornewMaisl-pQTMrepawraetderb.y TdihsesoplHvinogf t5h0emsgtotceksstosluutbisotnanwcaesi6n.31.00AmnL osmotic adjustment was made using NaCl dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear and colorless". Al test solutions were made by proportional dilutions...
Exposure vessels: Not given.
--
NNuummbbeerr ooff creopnlciecnattreast:iotnwso: Four plus a blank control
Element basis: Percent ight loss
002288
R0es0 wts00000000000
Nominal concentrations: Blank control, 31.25, 62.5, 125, and 250 mg/L
Element value:
155--mmiinnuuttee EECCsspo == 211476 ((124135 ---- 211499)) mmgg//LL 30-minute ECso = 124 (118 -- 131) mg/L
Element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not
the fluorochemical proportion alone.
c onclusions
The test substance 30-minute ECs for Photobacterium phosphoreum was determined to be 124 mg/L with a 95% confidence interval of 118 to 131
mg/L.
`Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
oaAQuAuTY
0000
Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution.
REFERENCES
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number P2581, 1996.
o0 mer000000000
Last changed: 6/26/00.
002289
05/26/2000
ENVIRONMENTAL LABORATORY STATUS REPORT
1
LAB REQUEST NO. P2581 REQUESTOR NAME: J MOHS-DAVIS PACE/SHARI LOUSHIN DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 08/14/1996 DESC: FC-GO3EF LIGHT WATER(TM) ATC 3%,AR-AFFF
CONTRACT LAB(S): PACE EXP COMP DATE: 11A/S1C8T/1996 PDARTOEJECCOTMPLLEEATDE:D:SA0B1E/A2C8H/1997 GPMHONFEAXNON:O: 665511--777788--76415726
206-6L-04; 7-7490. Standard Testing. Formulation is on document #07-7297-0. Results from Microtox Toxicity Test:
Test Conc. ng/L 31.25 62.5 125 250
Percent Light Loss
s-min Exp. 15-min Exp.
5.1
9.6
14
20
35
as
54
66
30-min Exp. 13 21 50 2
50 95% C.1.
220 (215 - 219)
146 (143 - 149)
124 (118 - 131)
sab 10/31/96
Results from Activated Sludge Respiration Inhibition Test:
Test Conc. mg/L 10 100 1000 1000 (dup)
Percent Inhibition in Respiration Rate 30-min Exp. 3-hr Exp.
0
0
0
0
0
0
0
0
EC50
> 1000
> 1000
sab 10/31/96
Results from Daphnia magna acute toxicity testing:
Test Conc. mg/L 62.5 125 250 500 1000
Percent Immobilization
24-hr Exp. 48-hr Exp.
0
0
0
0
0
0
15
60
25
85
cs0 95% C.1.
> 1000 -
480 (380 - 610)
NOEC*
250
No Observed Effect Concentration
Results from toxicity test with Selenastrum capricornutum (green algae):
Test Conc.
Percent Inhibition in Average Specific Growth Rate
-002290
mg/L
24-hrs Exp. 48-hrs Exp. 72-hrs Exp. 96-hrs Exp.
05/26/2000
ENVIRONMENTAL LABORATORY STATUS REPORT
2
LAB REQUEST NO. P2581
COMMENTS CONTINUED
31.25 62.5 125 250 500
0
12
0
3
1
12
1
7
53
15
4
9
56
40
a1
36
82
79
ot
85
EC50 95% C.I.
170 (150 - 200)
290 (250 - 330)
275 (250 - 300)
290 (265 - 330)
NOEC*
125
No Observed Effect Concentration.
Effects were determined to be algistatic.
sab 12/2/96
Results from Fathead Minnow acute toxicity test:
Test chambers aerated with gentle, single-bubble aeration due to loss of dissolved oxygen observed in range-finder studies.
Test Conc. ng/L 130 216 360 600
Percent Mortality
24-hrs Exp. 48-hrs Exp.
0
0
0
0
0
0
0
0
72-hrs Exp. 5 0 0 5
96-hrs Exp. 20 5 10 15
Leso
> 1000
>1000
> 1000
> 1000
sab 1/27/97
SAMPLE DATE CODE
DESCRIPTION RESULT
1
FC-6O3EF AMBER LIQUID
P-CBOD-05 BOD-05 (CARBONACEOUS)
P-CBOD-10 BOD-10 (CARBONACEOUS)
P-CBOD-20 BOD-20 (CARBONACEOUS)
P-CBOD-28 BOD-28 (CARBONACEOUS)
PACE COD
*C0D
ASCI IC50-ALGAE *ALGAE ASSAY - IC50
BOD-REVIEW BOD TESTING REVIEW
ASCI 48-HR-EC50 *DAPHNIA ACUTE TEST
ASCI 96-HR-LCS0 *FISH ACUTE TEST - 96 HOUR
MICROTOX MICROTOX - EC50
SLUDG-RESP SLUDGE RESPIRATION - EC50
216000 MG/KG 376000 MG/KG 446000 MG/KG 602000 MG/KG 740000 MG/KG 290 MG/L COMPLETE 480 MG/L >1000 MG/L 124 Mo/L >1000 MG/L
2
OUP #1
sermons
MOL or (95% C.I.)
2500 (265 - 330) (380 - 610) (118 - 131) -)
oT 002291
P-CBOD-05 BOD-05 (CARBONACEOUS) * = CONTRACT LAB DATA
110000 MG/KG
05/26/2000
ENVIRONMENTAL LABORATORY STATUS REPORT
LAB REQUEST NO. P2581 SAMPLE DATE CODE
CONTINUED DESCRIPTION
RESULT
2 P-CBOD-10 P-CBOD-20 P-CBOD-28
UP #1
Sree
BOD-10 (CARBONACEOUS) BOD-20 (CARBONACEOUS) BOD-28 (CARBONACEOUS)
326000 MG/KG 439000 MG/KG 318000 MG/KG
3 MOL or (95% C.I.)
00229
BEST COPY AVAILABLE
3
"7
ENVIRONMENTAL LABORATORY:
OR
ane
MICPR RC EPO SSHET EATG OSX
ISEEE:
Lal
ee
b=NTEH ST SR UBSTANCE:e - olson| b\ 37 8 LT E EeR nR RNiR
| meesgeeggl 0 SAE Fripuiatan igor Bins 4 Bo
SEL
RR
kx
E+
M FinalDila iiToenVe tSoulbo uie cr:eDS, A:i O. aEDOeRITsGv|r
C se e Ee
tomers ALO) pg
SRE
reSo a a
:
LE
fos.
J
3% Lr
e ap e eco kSoeconneSwOsM& ,ih Hi E
6 ie pe H Adjisiea d ih SN ------Bir) -- aREa Ol
Ba l atosmn fo 0 ree0r ld
Le oe on BERANEEeA
3
BL ne RTOTHE
BR REE Gio
haceiod
--
| EXChnae
ued toc Suintion
TT
Ax SE
1a
|
En
002293
BEST COPY AVAILABLE
LoTR ty ESdg t
l he y
israel. 8
MICROBTaOsXicDATTAesRtE:PORT
cae Te
e a aTee ne
again ene aes
est Re Li
Fe rie: g mnie Ee
bd jastNnacehnt
Pres
3
AT Gt SE re
RRIES
- NUMBE]
10/17
ais
HEE
ARS
fL RoOEiG e iCe aaGtonntioi) e BTE25R/ L92.E
$0
pr
TTaB0tSeNirEeYS+d e0.5780 ns tona
]
0 Oso edn HOUBmRE Ea
1
BE aEa nRO hc s ho o os een ) ss Te 760s45 AR G RI a HIB2IR0i t 00 t1 nRatdieegina es fpeieall] 4
fFo e 0 5 on0asiTozss 57 er siUgTRm0a0:0itgoo5b5u0sm aneaBo e3t 1
E Peo s T Ladn ana2ya i E 23 E200 RART AsG ri
ff Cocorrl icTe ion n FACTOR = 0T 841E slSioprG e T site ed
| Fads CSoapneEsaTa oh Ge/dL (95x CONEXTDEHNGRE FEAENGECLIN1E ionLRiAGArs T EABEe
j[PilEEieasnos spi aSonmita
e aoR sa SE Ee L SE bi
; B a ei n BY CEE EeeE E
;
ib AR 7e 8 L atsa54
ATL R s
:
PERE SSa E TN L PRE
fnBA
Zo jr
3
002294
BEST COPY AVAILABLE
3 ror sion RE
7
,
py
C (FL ERi TSaae ee BY isBasic TestTE
ee | Cae jal VEEL
C UAEe
"
3%
Test'Time: Je binutes hy Sh
+a sienNo osl eRi inn SEFAR RMEE+"
$0smotic Adjustnent:NaCLi
conc:
cr/canna PEEHES. SiST
.
rekE s a S13 i eCoeC aTno aTeTaieln]eeaeoet5eda 0sr e8bos8o gaheagsgl eate 8t g3
T0al0o
31.2500
;
11.00017874& ZLOEEa LEER
0.112 harSe
xpil
i Zod aa atlRelseoa lRaoldlIiaBtioo f=k Tseh
sL6a2lT0s s00G00000,1i
00]214054 0 240
oeoAARiE)Sk
FS o h Re n nitS ReRe E tSp 850 i00g e000n0Rto1lD R o T e
i
glo0 omhus=edcor conil eCHs an MEANS Hn
Be a
bEdi voooie AE RE BR REEels ice 1059300 10 agi
p bP EeLUni R oelRE SS aEaE iRR J S E SRL OiTs ESlTIe i oh LE e0nl s 7r2ae 7eGas
fo Se
; Sn Ve
|
ied LAE
=a
J
002295
BEST COPY AVAILABLE
: EA
SE
po TOT
bFalees i
atEee renorox para nepoer 81 l4aC l rEd h
be$I HmalEe eLKiL na G Basic Test "Tyiio {piohs eiint ANHoe
ANE TR AR Siale, nL SS
Honetinse t30 mifutes HE GEE
been Ron
TT
GamotickAfnudtment
edict 12 di
NaC
2
IEE EN
OTHE
B k od SiL anVaitCaobnh RueacliaSfai2em8sa/01i3/,059n8.1256
e p0.i0Eahc iaCgora iny fwn a l ]
hs SrEe ECon eldRgeis 20e017071445..10300s
E 831356030 2O000
BG < 2%
SAoviax faton)
LAH uF 43 IL
A
fS oT o o a S Eg en soTe a eee)e [4227/Bn20s8728e)
215505;s.0h000o00n00,t4 GgSietpmGos 418t010 H1n irdE. RDRSE
aso cong BECHER
EGR CofGRREGIION FACTO=R 1.0610 { FEY editor caeLations
Come tee LS
ea
ba ERI Gti we AE LE R
&aReE si Rn: TT
SLR ol Loy
PR ER J
est vate0 (SEER) 4
jC a n o E aE E S la en H SmE e RR SE
i
LL
Rjuuslia
S LR eR
RE
i
Bsus
j
|
002296