Document 060r2ELLxD42rM7yR21nNL4oV

METHODS AND MATERIALS This inv estig atio n was perfformed a t the aquatic toxicology l s a u b s o c reapt.toirbyi l -if ity Bi o o f nomi rain cs, bow |int rco.u, t Wareham, (Salmo`g M ai as rd sach neri us ) e t tt o s. The 8230 . (a light brown liq u id tested as 100$ activ e) was measured in terms o f the median tolerance lim it (TLcq) , the nominal c o n c e n t r a t i o n o f the chemijcal in Water w'h ic h c a u s e s 56 p e r c e n t response (death) under th^ test conditiqps-during a specified tim e i n t e r v a l . The p r e d i c t i o n o f a TL^0 v a l u e , and i t s 95% confidence in te rv al, was ta'sd on conversion of the concentrations tested and the corresponding observed .percent m ortalities to logs and probits respectively, and the subsequent mathematical c a l c u l a t i o n o f a ' Iiiidir`/ir ^ ^ |^ - -'e q u 8 ,' o |} . -The t o x i c i t y o f th e c h em ical t e s t e d was e v a lu a te d under s t a t i c b io a s s a y conditions for a 96-hour period. . The t e s t p r o c e d u r e s f o r th!e s t a t i c bioassayis a r e i n c o m plete acco rd a n c e w it h th e P ish E=ioassay P roced ure in t h e 1970 e d i t i o n o f s t a n d a r d Methods (APHA) e x c e p t f o r t h o s e c o n d i t i o n s described below. i I .Company Sanitized. Does not contain TSCA CBt : -fe :- W o The rainbow tro u t used in these te s ts were obtained from a commercial f i s h hatchery in M assach u setts and had a mean w eig ht of 1 .3 g and a mean le n g th o f 58 mm. The t e s t population was held in the laboratory hatchery f a c i l i t i e s io r a t le a s t 30 days p rio r to te s tin g and during th is pei led m o r t a l i t y was <2%. S t a t i c b ic a s s a y s were con d ucted in 5- g a l l o n glass vessels held in constant,temperature water baths at (~ 1*0). The v essels were not aera te d , and there was a single in tro d u ctio n of the te s t compound in to the te s t v essels. A solvent control, u tiliz in g the g reatest amount of isopropyl alcohol contained in any te s t vessel, was conducted. Fish were acclimated to the te st system containing reconstituted water as the standard diluent for a t least 2k hours prior to testin g . ien fis h were exposed in each t e s t v e s s e l. The r e c o n s t i tu t e d w ater used in th e t e s t system was prepared cy adding the fo llo w in g chemicals to deionized water: Hardness 38 rng/l NaHCOm^g of s CaalStO/ ^l i t e r wMgaStOe^r k8 30 30 K^l 2 The pH of th e s t a n d a r d d i l u e n t was 7 .1 , and the m ethyl o rang e a l k a l i n i t y was 35 ppm as CaCOy D is s o lv e d oxygen values for the various test vessels ranged from 6.? i n i t i a l l y to 5*3 m g/l a t t.he end of the t e s t s . jCompany Sanitized. Does not contain TSCA CBS IT.e p r e d i c t e d TL^0 v a lu e s (95% c o n fid e n c e i n t e r v a l ) a r e p r e s e n t e d in l a b l e 1 . T'able 2 p r e s e n t s a summary o f o b se rv e d m o r t a l i t y f o r the b io a s s a y a f t e r 2k and 96 h ours o f e x p o s u re . A g e la tin o u s suspension was formed immediately a f t e r in tro d u c tio n of the chemical, and various methods of mechanical s tirrin g would not a lte r the substance. Company Sanitized. Does not contain TSCA CBf '.ni i-Y W7B9ia0or nomic ehtfaamit,: s, ]nc. MS tarseseatchu s* 11 June, 1973 ~ PREPARED BY: R o b e rt E. B e n tle y AFPROVED BY: Kenneth j. Macek, Ph.D. Director Company Sanitized. Does not contain TSCA CB7 '.'3ole 1 - - Acute t o x i c i t y of 8230 to rainbow t r o u t 3 (Jlalmo g a ird n e rj ) rhese data are based on r e s u l t s of b io a ssa y s conducted at: th e a q u a t i c t o x ic o lo g y l a b o r a t o r y o f B ionom ics, I n c ., Wareham, M a ssa ch u se tts. Compound 8230 TL^0 - m i l l i g r a m / l i t e r 2^ hour 96 hour >1000 >1000 LN( roengv/Ee1lf f ) e c t 1000 aAssay conducted a t llC (- i . 0 ) , mean w eight o f rainbow t r o u t 1.3 g. , Company Sanitized. Does not contain TSCA CBf Table 2 __: o ru e n ti at,ions ol S230 t e s t e d and c o r re s p o n d in g observed percent m ortality for rainbow trout rsalmo g aird r.eri) at and 96 hours d u rin g a s t a t i c M oassay . Compound 82jC Con(cmegn/tlr)a tio n 1000 750 5~60 490 370 280 210 100 control - control (isopropyl alcohol) 245 hmoourrta lity yfocbhsoeurvred 00 00 00 00 00 00 00 00 00 00 Company Sanitized. Does not contain TSCA CBt