COMMONWEALTH OF KENTUCKY HANCOCK CIRCUIT COURT SHIRLEY H.

S. cien~ tist : Paul Connolly Technical Lead-LC/MS Christine Edwards Techaician Mark Ammennan Sample Custodian Amy Sheehan Associate Scientist Brittany Kravets .

However, because of the fact that uric acid and PFOA/PFOS were measured at the same time, we cannot know if an increase in either PFOA or PFOS would lead to an increase in uric acid.

The following personnel from Exygen Research were associated with various phases o f this interim portion o f the study: Name John Flaherty Karen Risha Paul Connolly Amy Sheehan Christine Edwards Brittany Kravets Mindy Cressley Krista Gallant Scott Crain Eric Hoover Carlyle Morrell Shawn Robb Mark Ammerman Eric Edwards Edward Cams Title Vice President Laboratory Supervisor Technical Lead-LC/MS Associate Scientist Technician Technician Technician Technician Technician Technician Intern Technician Technical Lead - Facilities Sample Custodian Sample Custodian Sample Custodian Exygen Research Page 6 of 132 Interim Report #10-Analysis o f Fish and Clam Samples Exygen Study No. : P0001131 TABLE OF CONTENTS Page TITLE PA G E ................ ........................................ ................................ .....................................1 GOOD LABORATORY PRACTICE COMPLIANCE STATEM ENT.............................. .2 QUALITY ASSURANCE STATEM ENT.................. 3 CERTIFICATION OF AUTHENTICITY............................. .....4 STUDY IDENTIFICATION................................... ....5 PROJECT PERSONNEL......................... 6 TABLE OF CONTENTS.................................................................................................... .......7 LIST OF TABLES............................ ............ ..............................................................................8 LIST OF FIGURES.......................................... .................... .......................................................9 LIST OF APPENDICES................................. .......................... .............................................. 10 1.0 SUMMARY............. ......................................... ............................................................. ...II 2.0 OBJECTIVE..................................... ........ .................................................... ....................12 3.0 INTRODUCTION................................................... ............................................. ............ 13 4.0 ANALYTICAL TEST SAMPLES.............. ............................................................... .13 5.0 REFERENCE M ATERIAL....................................................................... 13 6.0 DESCRIPTION OF ANALYTICAL M ETHOD............................................................15 6.1 Extraction Procedure............................... ............. ........................................................15 6.1.1 Sample Preparation............................................................. ....................... ....15 6.1.2 Glassware Preparation............................................ ..15 6.1.3 Column Preparation.............................................. 15 6.1.4 Sample Extraction..... ......................................................................................15 6.2 Preparation o f Standards and Fortification Solutions..................................................16 6.3 Chromatography.............................................................................. ......... ....... ............. 17 6.4 Instrument Sensitivity.............................................................................. 17 6.5 Description o f LC/MS/MS Instrument and Operating Conditions............................17 6.6 Quantitation and Example Calculation..................... .............................................. .....18 7.0 EXPERIMENTAL D ESIG N ............................. ......................... ....................................20 8.0 RESULTS................................. ............. ........... ................... .......................................... 20 9.0 CONCLUSIONS................... ................................................................................ ........... 21 10.0 RETENTION OF DATA AND SAMPLES ....... ......... ...................... ........................ .21 Exygen Research Page 7 of 132 Interim Report #10-Analysis of Fish and Clam Samples Exygen Study No.: P0001131 LIST OF TABLES Page Table L Summary of PFBS, PFHS and PFOS in Fish Samples......................................... 23 Table II.

107 f AR 26 _ 1126 REVISED DRAFT HAZARD ASSESSMENT OF PERFLUOROOCTANOIC ACID AND ITS SALTS s ._. = =2 x 25 = a U.S.

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EOS-0210 Interim Report #18 Northern Alabama Potable Witter Systems, December 2005 Interim Report #18: Analysis of Water Samples from Northern Alabama Potable Water Systems, December 2005 Study Title A nalysis of Perfluo robutanesulfonate (PFBS), Perfluorohexanesulfonate (PFHS), an d Perfluorooctanesulfonate (PFOS) in Wate r , So il, S edim ent, Fis h , Clam s, V eg etatio n , Sm all Mam m al Liver an d Sm all Mam m al S erum Using LC/MS/MS FOR THE 3M DECATUR MONITORING PROGRAM Data Requirement EPA TSCA Good Laboratory Practice Standards 40 CFR 792 Author M ichelle D.

EOS-0210 Interim Report #18 Northern Alabama Potable Witter Systems, December 2005 Interim Report #18: Analysis of Water Samples from Northern Alabama Potable Water Systems, December 2005 Study Title A nalysis of Perfluo robutanesulfonate (PFBS), Perfluorohexanesulfonate (PFHS), an d Perfluorooctanesulfonate (PFOS) in Wate r , So il, S edim ent, Fis h , Clam s, V eg etatio n , Sm all Mam m al Liver an d Sm all Mam m al S erum Using LC/MS/MS FOR THE 3M DECATUR MONITORING PROGRAM Data Requirement EPA TSCA Good Laboratory Practice Standards 40 CFR 792 Author M ichelle D.

EOS-0210 Interim Report #18 Northern Alabama Potable Witter Systems, December 2005 Interim Report #18: Analysis of Water Samples from Northern Alabama Potable Water Systems, December 2005 Study Title A nalysis of Perfluo robutanesulfonate (PFBS), Perfluorohexanesulfonate (PFHS), an d Perfluorooctanesulfonate (PFOS) in Wate r , So il, S edim ent, Fis h , Clam s, V eg etatio n , Sm all Mam m al Liver an d Sm all Mam m al S erum Using LC/MS/MS FOR THE 3M DECATUR MONITORING PROGRAM Data Requirement EPA TSCA Good Laboratory Practice Standards 40 CFR 792 Author M ichelle D.

"McCrea, Deborah" <mccrea@taftlaw.com> 06/12/2009 03 :54 PM p. 1 To cc bcc Subject AP aas-3gq3 NCIC OPPT@EPA .

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AR226-3160 TRADE SECRET Study Title H-23925: Biopersistence Screening 10-Dose Oral Gavage Study in Rats Laboratory Project ID: DuPont-2921 AUTHOR: Carol Finlay, B.A.

"fRev. 1/94) Subpoena In a Civil Case Issued by the UNITED STATES DISTRICT COURT EASTERN _______________ DISTRICT O F ______MISSOURI.

A t ^ 3-6 "McCrea, Deborah" <mccrea@taftlaw.com> 06/12/2009 03:54 PM To NCIC OPPT@EPA cc "Bilott, Robert A."