AR226-3106 H n tin g d o n V CONFIDENTIAL DPT 437/984087 i ACTIVATED SLUDGE - RESPIRATION INHIBITION TEST Sponsor Dupont Speciality Chemicals, Jackson Laboratory, Chambers Works, Deepwater, NJ 08023, USA.

AR226-3166 ____________________ ____________________________ DuPont-4182 TRADE SECRET Study Title H-24159: Biopersistence Screening 10-Dose Oral Gavage Study in Rats Laboratory Project ID: DuPont-4182 Author: Carol Finlay, B.A.

M .M & 414 TRADE SECRET Study Title H-24768: Developmental Toxicity Study in Rats Laboratory Project ID: DuPont-6515 DuPont-6515 T e s t G u i d e l i n e s : U.S.

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b -- --------------------------------------------- ------------------------------------------------------------------------------ 498 fa ite 3 3 DuPont-13882 TRADE SECRET Study Title H-26137, H-26138, and H-26139: Repeated Dose Dermal Toxicity 28-Day Study in Male Rats Test Guidelines: U.S, EPA Health Effects Test Guidelines OPPTS 870.3200 (1998) OECD Guideline for the Testing of Chemicals Section 4: Health Effects, Number 410(1981) Author: Carol Finlay, B.A.

AR226-3327 I Surface Protection Solutions 6-May-2004 Com pany Sanitized.

AR226-3351 - Continuous flow-through - 14 volume renewals/dav - Negative, positive control (PFOS), 4 test substances - Single test concentration ~ 10 pg/L as total fluorine - nominal test substance cone's from 13 - 100 pg/L -1 test chamber/test substance; 70 fish /test chamber - 16 L : 8 D photoperiod - 14 d uptake phase, 14 day depuration phase - Fish fed - 2% body weight; frozen brine shrimp - Loading - 0.2 g/L/day .esu Stock Solutions - % Recovery of Test Substance as Fluoride a DayO @Day 13 esults PFOS Uptake and Depuration O Measured m Modeled Uptake 4 Modeled Depuration Fluoride, ug/g Day Results 8.000 Urethane Polymer Uptake and Depuration o Measured Modeled Uptake a Modeled Depuration 7.000 6.000 5.000 Fluoride, ug/g 4.000 3.000 2.000 1.000 0.000 o o $ oo 10 O O <0 ----- A A A A A Si 00 AA 15 20 Day Fluoride, ug/g Resulti Zonyl BA (Telomer B Alcohol) Uptake and Depuration O Measured Modeled Uptake 4 Modeled Depuration 14.000 12.000 10.000 8.000 XO>- 6.000 4.000 2.000 0.000 -c O -ooo o o 10 oo o o 15 Day OO 20 -ft25

EH.aI.skdeulPl oLnatbdoerNateomryofuorrsHanedalCthoamnpdaEnnyvironmental Sciences 2 December 2004 Toxicokinetics in the Rat: Pilot results Study Director: W.J.

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AR226-3560 ANDREA V.

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14) OECD 202-OPPTS 850.1010, 48-hour static acute toxicity test with the Cladoceran (daphnia magna), 454A-118A PERFLUOROBUTANE SULFONATE, POTASSIUM SALT (PFBS): A 48-HOUR STATIC ACUTE TOXICITY TEST WITH THE CLADOCEFAN (Daphnia magna) FINAL REPORT SANITIZED WILDLIFE INTERNATIONAL, LTD.

Katherine E.

ATSttCn:A SDeocctuiomne8n(et)P/FroYcIessing Center Attachment 1 P-1 P-1 July 28,2006 Page 4 of 5 M Z26 _373J- Acronym codefor Tables 1 and 2 In Tables 1 and 2, the following acronyms are used: acronym meaning CW FW WW DuPont Chambers Works facility in Deepwater, New Jersey DuPont Fayetteville Works facility in Fayetteville, North Carolina DuPont Washington Works facility in Parkersburg, West Virginia PPPFFFHPBeAxAA PFHpA PPPPFFFFOUDNnAAAA PFDoA PFBS PPPFFFOHOSxSSA PPeerrfflluuoorroobpuentatannooicicaacicdid Perfluorohexanoic acid Perfluoroheptanoic acid Perfluorooctanoic acid Perfluorononanoic acid Perfluorodecanoic acid Perfluorotmdecanoic acid PPPeeerrrfffllluuuooorrrooobhdueoxtdaaennceaenssuoulilfcfooanncaaitdtee Perfluorooctane sulfonate Perfluorooctane sulfonamide Attachment 1 Spaed Matrix Lab Blank 1 cw # P % cw p 3 cw n m 34 CW (DUPLICATE) c w v 5 cw i cw < P * CW 7 cw qp ( CW WG18817-102 Y2 WG18817-101 Y1 L8789-1 Y1 LB789-2 Y1 L87B9-3 Y1 WG18817-103Y1 L8789-4 Y1 L8789-5 Y1 LB789-5 Y1 LB789-6 Y1 L8789-7 Y1 L8789-8 Y1 % Recovery ng/mL ng/mL ng/mL ng/mL ng/mL ng/mL ng/mL ng/mL ng/mL ng/mL ng/mL WQ18817 W Q 18817 WQ18817 WQ18817 WG18817 WG18817 WG18817 WG18817 WG18817 WG18817 WG18817 WG18817 PFBA 81.2 ND ND ND ND ND ND <1.44 <1.44 <1.44 NO ND PFPeA 79.0 ND ND ND ND ND ND ND NO ND ND ND PFHxA 85.8 ND 1.82 ND <0.900 <0.900 ND ND ND <0.900 ND <0.900 PFHpA 83.5 ND ND ND ND ND <1.41 3.71 3.71 11.7 6.91 ND PFO A 110 ND 7.71 9.72 24.1 21.6 42.8 13.4 13.4 111 56.7 26.6 PFNA 11B ND 4.28 2.90 7.10 6.78 18.3 4.22 4.22 53.1 29.2 2.37 PFDA 115 ND <1.06 <1.08 1.64 1.51 8.47 1.82 1.82 13.5 7.47 <1.08 PFUnA 128 ND <1.02 <1.02 2.37 2.60 1.70 <1.02 <1.02 3.99 1.55 ND PFDoA 60.9 ND ND ND <0.600 <0 600 0.943 <0.600 <0.600 1.10 0.737 ND PFBS 94.8 ND ND ND ND ND ND ND ND ND ND ND PFHxS 104 ND <3.72 <3.72 39.1 33.5 <3.72 <3.72 <3.72 4.28 <3.72 3.81 PFOS 112 ND 51.1 35.3 68.2 58.0 25.7 35.0 36.0 39.0 53.8 36.8 PFO SA 73.5 ND ND ND ND ND ND ND ND NO ND ND Attachment 1 PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA tp // CW ^ k F w t f f lV L8789-9 Y1 LS789-10Y1 L8789-11 Y1 ngftnL ngAnL WG1B817 WG18817 ngAmL WG18817 ND ND NO ND ND ND ND ND ND NO 1.48 ND 12.9 13.0 28.3 <1.53 3.97 2.28 ND <1.08 <1.08 ND <1.02 <1.02 ND ND ND ND ND ND <3.72 <3.72 6.64 30.7 46.9 37.2 ND ND ND t-2.