Document zdgy0xQL9Dz65oo2VkXEkDD36

HAZLETON LABORATORIES AMERICA, INJC. M O trO U n Chemical & BioMedical Sciences Division 3301 K IN S M A N BLVD. P.O. B O X 7545 M A D IS O N , W IS C O N S IN 53707 P H O N E (608) 241-4471 .,-TtX 7 0 3 9 5 6 > IA Z R A l M D S UD y'' **''* // * / OCT i ]fc~,0c * REPORT OF ANALYSIS W. C. MCCORMICK MINNESOTA MINING AND MANUFACTURING TOXICOLOGY SERUICES ST. PAUL. MN 55101 SAMPLE: T-3607 PURCHASE ORDER NUMBER: P-688709-405 SAMPLE NUMBER: 407039 DATE ENTERED: 07/19/ REPORT PRINTED: 10/05/ ENCLOSED: ACUTE ORAL TOXICITY STUDY - METHOD, SUMMARY, PATHOLOGY RAW DATA APPENDIX SIGNED: STEUEN M. GLAZA STUDY DIRECTOR ACUTE TOXICOLOGY U BY AND FOR HAZLETON LABORATORIES AMERICA, INC. RAW DATA FOR THIS STUDY ARE KEPT ON FILE AT HAZLETON LABORATORIES ' AMERICA, INC. MADISON, WISCONSIN. 000074 Chemical & BioMedical Sciences Division 3301 K IN S M A N BLVD. P.O. B O X 7545 M A D IS O N , W IS C O N S IN 53707 P H O N E (608) 2414471 T LX 703956 H A Z R A L M D S UD SAMPLE NUMBER: 40703983 SAMPLE: T-3607 PAGE ACUTE ORAL TOXICITY Test Animal: Young adult male and female albino rats (approximately 7 weeks of age) of the Sprague-Dawley strain were procured, maintained group cages in temperature- and humidity-controlled quarters, provided continuous access to Purina Rodent Chow and water, and held for an acclimation period of at least 7 days. in Acclimated animals were chosen at random for the study. Test animals were housed by sex in groups of five and identified by animal number and corresponding ear taq. Food and water were available ad libitum throughout the study, except for an overnight period just before test ma terial administration when food, but not wa ter, was withheld. Reason for Species Selection: The rat is the animal classically used due tc its small size, ready availability, and large amount of background data. Method: Five male and five female rats weighing between 202 and 231 g were used for a sinqle dosage level of 5.0 a/kg of body weight. Preparation and Administration of Test Material: An individual dose was calculated for each animal based upon its fasted body weight and admin istered undiluted by qava ge. The dose volume was 2.76 ml/kg of body weight based upon the average bulk density of 1.81 g/ml. Observations: The animals were observed for clinical signs and mortality at 1, 2.5 and 4 hours following test material administration. The animals were observed daily thereafter for 14 days for clinical signs and twice daily for mortality. All animals were weighed just before test material administration, at 7 days and at study termination. Pathology: At study termination all animals were euthanatized, subjected to a gross necropsy examination and all abnormalities were recorded. 000075 @ HAZLETON LABORATORIES AMERICA. INC. Chemical & BloMedlcal Sciences Division 3301 K IN S M A N BLVD. P.0. B O X 7545 M A D IS O N , W IS C O N S IN 53707 P H O N E (608) 241-4471 T U 703956 H A Z R A L M D S UD SAMPLE NUMBER: 40703983 SAMPLE: T-3607 ACUTE ORAL TOXICITY (CONTINUED) SUMMARY Test Animal: Albino Rats - Sprague-Daw1ey strain Source: Harlan Sprague-Daw1e y , Madison UI Date Animals Received: 07/24/84 Method of Administration: Oral Gavage Date Test Started: 08/03/84 Date Test Completed: 08/17/84 Estimated Oral LD5 0 : Male - Greater than 5.0 g/kg of body weight Female - Greater than 5.0 g/kg of body weight Mortality Summary (Number of Deaths) Dosage Leve 1 (q/ k o ) 5.00 Hours 0-4 MF 00 1 MF 00 2 MF 00 Days 34 MF MF 00 00 5 MF 00 6 MF 00 7-14 MF 00 Tota 1 M F Bo th 0/5 0/5 0/10 Average Body Weights (g) Initial Day 7 Terminal Ma 1e 221 276 295 Fe m a 1e 210 226 231 PATHOLOGY An ima 1 Number C21437 C21439 C21434 C21433 C21432 Test Day Sex Died Sacr if iced M - 14 M - 14 M - 14 M - 14 M 14 C21521 F - 14 C21503 F - 14 C21522 F - 14 C21523 F - 14 C21524 F - 14 Necropsy Comments No Uisible Lesions. No Uisible Lesions. No Uisible Lesions. No Uisible Lesions. Lungs - multiple, raised, grey f on all lobes, pinpoint to 2 mm i d iameter. No Uisible Lesions. No Uisible Lesions. No Uisible Lesions. No Uisible Lesions. No Uisible Lesions. 0000V6 Reference: Hitch, R.K., "Acute Oral Toxicity Study," Pesticide Assessment Guidelines, Subdivision F, Hazard Evaluation: Human and Domestic Animals, U.S. Environmental Protection Agency Office of Pesticide and Toxic Substance Series 81-1, pp . 34-39 (November 1982). GROSS CLINICAL OBSERVATIONS Study Tittle: f l f 'i j J - * A / 'O J / O A i f l i J - L j ---- Testt Article: 1~~ 3A>/0"J * ^*3, Dosage Level: Q-C)C^ j0j~ Vehicle: AJA . ------ Species: f i n k ________ _____________ Dose Time: _ _ _________ ^ r * 3 o ------------------- S#xs ---- ------------- ----- 000077 GROSS CLINICAL OBSERVATIONS study T itle: m J r e f r r a L T .M i+ c j. --- ---------------- --------------- Test Article: Dosage Level: T ' ________________ K No. .fljflZ j ) . b 9-/ ^ _____ Vehicle: ____ &L_____________ Species: ___ ___________________________ Dose Tine: Sex: __ _____ _ 000078 ( Test Material o ACUTE ORAL TOXICITY (LD5Q) RECORD _________________ Vehicle AJA___________________ RT No . o Bulk D e n s i t y --- (g/ml) Species fgjt Source t-h? ______ Date Received_Z^ZilSL Dosage S'.O (g/kg) Fasted: Date . Time 3 36piTech. Room No. 3 tom Dose Volume 3-76?(ml/kg) Dose Time jK Z lD Mt_______ Tech. Date Scale Used: Sex Animal No./Ear Tag Noj.2. IM3 7 |4qQ f W w \Prefasted Bodv Weight fcl M iq $2 V \ \Fasted Body Weight (g) m 0-ltO \Actual Dose (ml ) b W IMtl ft.h D-ltO VDay 7 Body Weight (g) \Day 14 Body Weight (e) P-tf/ 3oo 7-lb 771 30? ?XSt 2Pfl Qmt . t-3 NA >? it m Jlp Sit=, NA W ;1b o n g/n titolo ><TOi4 Doses VerifjLed by --Sdik- ___ NA______ BEST COPY AVAILABLE 000079 Dosage S'.> (g/kg) iW 4- Dose Volume (ol/kg) Dose Time // '*20 ______ Tech. Date Scale Used: Animal No./Ear Tag No.AZ l'Vtf Prefasted Body Weight (g) A)4 Fasted Body Weight (g) Actual Dose ( ml ) Day 7 Body Weight (g) Day 14 Body Weight (g) r7lY am \h.lo h.Ub \\23 sr i l l *__ 1^3 |33L*J \\\Tia 303 \t).V o/S7 0 % \731 ?s>o \Z3 o Z2> Z2-V vjo ... $ls JVrt3.. VYf\ Y8*p {k Vp 5 o 5I n NA -- ------^ hKtiDtQ . NA rihm & BP j s oiq _ , Boaea-Verlfied by .f9\4w ___ NA______ MORTALITY (NO. DIED/NO. DOSED) 2 6Dose Level Hours 0 -4 1 Study Day ___________________________________________ 34 5 7 8 9 1 D il 12 13 14 Total s'.d y X D/s; Technician ^3 DateW,$M m am *& 1 pm ea/sm pm * l am % fr t fil? Jl) A % IL 1 V %% f am nm an pm %K % %fcb %' kh %& k>4 h f e m % (a i pm an 9t k f - (il 4%k%S % am nm fi /%f * i& % fpm. am pm am pm am pn t % %% IA . & % m % 4 is sf K% & HA M. h M k V if % 1? NA 9k h `' / s NA - Not Anolicabl* toMltr'LyJLnr'Qr V z - x i j p Raviewej hy pi^ *7/3 -j