Document zdD75BOKoDzK77dpm6QzYQMQ7
Attachments to Letter to C. Auer dated May 18,2000 Studies and Other Information on Certain
Perfluorooctane Sulfonate-Related Compounds
j\ W X X )
4. PFDS
Perfluorodecanesulfonate
Acute Toxicity
1) Final Report, Acute Dermal Toxicity Study in Rabbits, Hazelton Laboratories America, Inc., 3M Reference No. T-4102, Sample No. T837389-410 754, January 21, 1988
2) Final Report, Acute Oral Toxicity Study in Rats, Hazelton Laboratories America, Inc., 3M Reference No. T-4102, Sample No. T837389-410 754, January 25, 1988
3) Final Report, Primary Eye Irritation/Corrosion Study in Rabbits, Hazelton Laboratories America, Inc., 3M Reference No. T-4102, Sample No. T837389-410 754, January 20, 1988
4) Final Report, Primary Dermal Irritation/Corrosion Study in Rabbits, Hazelton Laboratories America, Inc., 3M Reference No. T-4102, Sample No. T837389-410 754, January 21, 1988
Genotoxicitv
1) Mutagenicity Test with T-6357 in the Salmonella --Escherichia coli / MammalianMicrosome Reverse Mutation Assay, Coming Hazleton, Inc. (CHV), Project No. 17387-0-409, 3M Reference No. T-6357, FC-120, April 1, 1996
2) Mutagenicity Test on T-6357 in an In Vivo Mouse Micronucleus Assay, Coming Hazleton, Inc. (CHV), Project No. 17387-0-409, 3M Reference No. T-6357, FC-120, April 23, 1996
Pharmacokinetic Studies
1) Final Report, Analytical Report and Single-Dose Dermal Absorption / Toxicity Study of T-6052 in Rabbits, Hazleton Wisconsin, Inc., Project No. HWI 6329-135, 3M Reference No. FC-120, T-6052 (0.02 % in water), November 20, 1995
2) Single-Dose Dermal Intravenous Pharmacokinetic Study of T-6052 in Rabbits, Hazleton Wisconsin, Inc., Project No. HWI 6329-134, 3M Reference No. T-6052 (0.02 % in water), November 20, 19995
- 6 - 0GC512
Attachments to Letter to C. Auer dated May 18,2000 Studies and Other Information on Certain
Perfluorooctane Sulfonate-Related Compounds Pre-1976 Studies (bibliography only) 1) Acute Oral Toxicity Study with T-1019 in Male Albino Rats, Industrial Bio-Test
Laboratories, Inc., Project No. 601-05394, 3M Reference No. T-1019, August 6, 1974 2) Skin Irritation, Eye Irritation, Acute Oral LD50, WARF Institute, Inc., Project No.
4053863, 3M Reference No. T-992, May 24, 1974 3) Acute Oral Cholinesterase Study with T-1019 in Male Albino Rats, Industrial Bio-
Test Laboratories, Inc., Project No. 601-05394, 3M Reference No. T-1019, August 6, 1974
-7- 000513
H A Z L E T O N LABORATORIES AMERICA. INC.
Chemical & BioMedical Sciences Division
3301 KINSM AN BLVD. P.0. BOX 7545 MADISON, W ISCONSIN 53707 ' PH O N E (608) 241-4471 TLX 703956 H AZR AL MDS UD
FINAL REPORT
BEST COPY AVAILABLE
ROGER G . PERKI NS MINNESOTA MINING & MANUFACTURING TOXICOLOGY SERUICES
ST. P A U L , MN 55101
COMPANY
SAMPLE: T-4102 PURCHASE ORDER NUMBER: T837389-410 754
SAMPLE ENTERED: 09/25/31 REPORT PRINTED: 01/21/81
ENCLOSED:
A C U T E D E R M A L T O X I C I T Y S T U D Y IN R A B B I T S (OEC D G U I D E L I N E S )
o Key Personnel o Method o Summary o Individual Pathology Comments o References o Pathology Report o Raw Data Appendix
SIGNED
S T E U E N M. G L A Z A STUDY DIRECTOR ACUTE TOXICOLOGY
....................Y M - & .
DATE
HAZLETON LABORATORIES AMERICA. INC.
Chemical & BioMedical Sciences Division
3301 KINSM AN BLVD. P.O. BOX 7545 MADISON, W ISCONSIN 53707 PH O N E (608) 241-4471 TLX 703956 H AZR AL MDS UD
RAMPIE NUMBER: 70905762 SAMPLE: T-4102
BEST COPY AVAILABLE PAGE 2
KEY PERSONNEL
Acute Tovicoloou
S t e v e n M. G l a z a Study Director
C a l v i n L. H o r t o n Group Leader Support Services
S h a r e n L. H o w e r y Report Coordinator
Quality Assurance
Debra Curley Arndt Manage r
Anatomical Pathology
T h o m a s E. P a l m e r , P h D Anatomical Pathologist
R o b e rt S a 1a v a Senior Section Supervisor
Dennis Hoffman Group Leader Nec ropsy
Anne Mosher Group Leader Pathology Data
Laboratory Animal Ueterinarian
C i n d y J. C a r y , D U M Diplomate, ACLAM
000515
H A Z L E T O N LABORATORIES AMERICA, INC.
Chemical & BioMedical Sciences Division
3301 KINSM AN BLVD. P.0. BOX 7545 MADISON, W ISCONSIN 53707 PH O N E (608) 241-4471 TLX 703956 H AZR AL MDS UD
SAMPLE NUMBER: 70905762 SAMPLE: T-4102
PAGE
BEST COPY AVAILABLE
OECD DERMAL SCREEN
Objective: To assess the systemic toxicity and relative skin irritancy o f a t e s t m a t e r i a l w h e n it is a p p l i e d to t h e s k i n a c c o r d i n g to t h e Organisation for Economic Cooperation and Development's Guidelines for T e s t ino of C h e m i c a l s .
Test Material: T-4102 Physical Description: Purity and Stability:
Storage and Retention:
Safety Precautions:
Dark amber liquid Sponsor assumes responsibility for
purity and stability determinations. T he test m a t e r i a l w a s s t o r e d at r o o m t e m p e r a t u r e . Any unused material will be discarded according
to HLA St a n d a r d O p e r at in g Procedure. Normal handling procedures were used according to HL A S t a n d a r d O p e r a t i n g P r o c ed ur e.
Test Animal: Young adult rabbits of the New Zealand White strain were p r o c u r e d , m a i n t a i n e d i n d i v i d u a l l y in s c r e e n - b o t t o m c a g e s in t e m p e r a t u r e a n d h u m i d i t y - c o n t r o l l e d q u a r t e r s , p r o v i d e d a c c e s s to w a t e r ad. l i b i t urn and a measured amount of Purina High Fiber Rabbit Chow, and held for an a c c l i m a t i o n p e r i o d of at least 7 days. A n im al h u s b a n d r y an d h o u s i n g at H L A c o m p l y w i t h s t a n d a r d s o u t l i n e d in. t h e " G u i d e f o r t h e C a r e a n d U s e o f L a b o r a t o r y A n i m a l s " , If v a r i a t i o n s f r o m t h e p r e s c r i b e d e n v i r o n m e n t a l c o n d i t i o n s ex isted, they were d o c u m e n t e d and c o n s i d e r e d to have no effe ct on the s t u d y ou tco me. No c o n t a m i n a n t s we r e e x p e c t e d to h a ve b e e n p r e s e n t in the fe ed or w a t e r w h i c h w o u l d h a v e i n t e r f e r e d w i t h or a f f e c t e d the results of the study.
Five male and five female acclimated rabbits, weighing between 2097 g and 2 3 45 g, were c h o s e n at random, t r e a t e d , and m a i n t a i n e d d u r i n g the o b s e r vation period as specified for the accl im at io n period. Test animals were identified by animal number and corr es po nd in g ear tag. A p p r o x imately twenty-four hours before test material application, each rabbit's back was shaved with an elec tr ic clipper. The shaved area made up a p p r ox im at el y 2 0% of the total body surface.
Reason for Species Selection: Historically, the New Zealand White a l b i n o rabbit, h a s b e e n the a n i m a l of c h o i c e d u e to the l arg e a m o u n t of background information on this species.
P r e p a r a t i o n of Test Material: The sample was dosed as received. An individual dose was calculated and weighed out based upon each animal's b o d y weight at initiation.
000516
H A Z L E T O N LABORATORIES AMERICA, INC.
Chemical & BioMedical Sciences Division
3301 KINSM AN BLVD. P.O. BOX 7545 MADISON, W ISCONSIN 53707 PH O N E (608) 241-4471 TLX 703956 H AZR AL M DS UD
SAMPLE NUMBER: 7G905762 SAMPLE: T-4102
PAGE
T COPY AVAILABLE
OECD DERMAL SCREEN
(CONTINUED)
T r e a t m e n t : The test m a t e r i a l was a p p l i e d to ea c h a n i m a l ' s ba ck at a do se level of 2.D g/kg. The area of a p p l i c a t i o n was c o v e r e d wi th a 10 x 10-cm gauze patch secured with paper tape and overwrapped with Saran Wrap and Elastoplast tape. Twenty-four hours later the bandages were removed and the backs were washed with lukewarm tap water and wiped with d i s p o s a b l e p a p e r towels. C o l l a r s w e re a p p l i e d to r e s t r a i n the test a n i m a l s
during the 24-hour exposure period.
R e a s o n f o r R o u t e of A d m i n i s t r a t i o n : H i s t o r i c a l l y , t h i s is the r o u t e of c h o i c e is b a s e d on the m e t h o d of D r a i z e .
Observations: Each animal was observed for clinical signs and mortality at 1, 2 . 5 a n d 4 h o u r s a f t e r test m a t e r i a l a d m i n i s t r a t i o n . T h i r t y minutes after removal of the test material the initial dermal irritation reading was made. Subsequent readings of dermal irritation were made on S t u d y D a y s 3, 7, 10 and 14. The a n i m a l s w e r e o b s e r v e d d a i l y for clinical signs and twice daily (morning and afternoon) for mortality.
The a n i m a l s were w e i g he d just prior to test ma terial ap pl ic a t i o n . Bo dy w e i g h t s w e r e t a k e n a g a i n at 7 d a y s a n d at. s t u d y t e r m i n a t i o n o r at d e a t h .
Pa t h o l o g y : At s t u d y t e r m i n at io n, s u r v i v i n g a n i m a l s were e u t h a n a t i z e d . All an im al s, w h e t h e r dying on test or e u t h a n a t i z e d at te rm in a t i o n , were s u b j e c t e d to a g r o s s n e c r o p s y e x a m i n a t i o n and all a b n o r m a l i t i e s we re recorded. After necropsy, animals were discarded and no tissues were
saved.
Statistical Methods: Other than average body weights, no other statistical method was performed.
Location of Raw Data and Final Report: The raw data and a copy of the final r e p o r t w i l l be r e t a i n e d in the a r c h i v e s of HLA.
H A Z L E T O N LABORATORIES AMERICA, INC.
Chemical & BioMedical Sciences Division
3301 KINSM AN BLVD. P.0. BOX 7545 MADISON, W ISCONSIN 53707 PH O N E (608) 241-4471 TLX 703956 H AZRAL MDS UD
SAMPLE NUMBER: 70905762 SAMPLE: T-4102
PAGE
5
BEST COPY AVAILABLE
OECD DERMAL SCREEN
(CONTINUED)
SUMMARY
Test: A n i m a l : A l b i n o R a b b i t s - N e w Z e a l a n d W h i t e Source: Hazleton Research Products, Inc., Denver PA Date Animals Received: 11/03/87
Method of A d m i n i s t r a t i o n : Dermal Ap plication
Date Test Started: 11/16/87
Date Test Completed: 11/30/87
Estimated Dermal LD50:
Male - Greater than 2.0 g/kg of body weight Female - Greater than 2.0 g/kg of body weight
Dose Leve l (G / K G )
2.0
Hou irs 0- 4 MF
00
1 MF 00
MORTALITY SUMMARY (NUMBER OF DEATHS)
Da vs 23456 MF MF MF MF M F
7- 14 MF
Tot a 1 M F Both
0 0 0 0 10
00 00
0 0 1/5 0/5 1/10
Male Ferna 1e
Average Body Wei gh t s (g )
Initial
Day 7
Terminal
2187 2193
1784 2148
1671 2374
000518
HAZUEXOIM LA BO RATORIES AM ERICA. INC
Chemical & BioMedical Sciences Division
3301 KINSM AN BLVD. P.O. BOX 7545 MADISON, W ISCONSIN 53707 PH O N E (608) 241-4471 TLX 703956 H A ZR A L MDS UD
SAMPLE NUMBER: 70905762 SAMPLE: T-4102
BEST COPY AVAILABLEAGE 6
O E CD DERMAL. S C R E E N
(CONTINUED)
Cl in ica 1 S i g n s (No. of A n i m a l s A f f e c t e d )
Hours 1 .0 2. 5 4. 0
____________________________ D a u s 1 2 3 4 5 6 7 8 9 1 0 11 12 13 14
Males
Appeared Normal 5 5 5 5 4 4 4 0 0 0 0 0 0 0 0 0 0
Thin appearance 0 0 0 0 1 1 0 0 0 1 1 1 3 3 3 3 3
H y p o a c ti v i t y
0 0 0 011 001333 3 3 3 2 3
Soft stool
0 0 0 000011111 1 0 0 1 1
Loss of
appiet ite
0 0 0 00 0 02 3333 3 3 3 2 2
Few feces
0 0 0 000033333 3 3 3 3 3
D ia r rh ea Death
0 0 0 0 0 0 00 0 0 02 2 1 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1-
F e m a les
Appeared Normal 5 5 5 5 5 5 5 4 4 4 4 4 4 4 4 5
Few feces
0 0 0 000011111 1 1 1 0 0
Loss of
appe t ite
0 0 0 000011111 1 1 1 0 0
Upon t r a n s f e r r i n g anim al s to the n e c r o p s y area, Anim al No. F2 Q5 75 a p p e a r e d to g o i n t o a s u b c o n u u 1s i w e s t a t e f o r a p p r o x i m a t e l y 3 to 5 minutes. At this time the an imal was n e c r o p s i e d as a m o r i b u n d
s a c r i f ic e .
Comments: Dermal irritation observed during the st ud y co ns is te d of slight to m o d e r a t e e r y t h e m a and d e s q u a m a t i o n and slight ed em a and fi ssuring.
000513
( ^ H A Z L E T O N LABORATORIES AMERICA, INC.
Chemical & BioMedical Sciences Division
3301 KINSM AN BLVD. P.O. BOX 7545 MADISON, W ISCONSIN 53707 PH O N E (608) 241-4471 TLX 703956 H A ZR A L MDS UD
SAMPLE NUMBER: 7090*5762 SAMPLE: T-4102
m COPY AVAILABLE PAGE 7
OECD DERMAL SCREEN
(CONTINUED)
PATHOLOGY
D o s e L e v e 1 : 2. 0 g / k g of b o d y w e i g h t
An ima 1 Number
Test Day Sex Died Sacrificed
Necropsy Comments
F20455
M
- 14
Perianal stains - brown; animal appears thin.
F2491
M
- 14
An im a 1 thin.
F2057
M
- 14
Perinal stains - tan; animal appears thin.
F20493
M
4
Treated skin - diffusely red; perineum - stained brown; colon contents unformed; cecum - contents d r y to impacted,? c e c u m an d ileum serosa has multiple red areas, up
to 5.0 x 2.0 cm.
F20564
M
*" 1 4
Colon - contains clear, frothy fluid; perianal stains - green: animal appears thin.
F20535
F
14 Perianal stains - green; subcutaneous tissue underneath treated skin - multiple red, p in p o in t foci.
F20558
F
14 Perianal stains - dark brown: left ax il la ry lymph node diffusely dark red; animal appears thin.
F2 0570
F
14 S u b c u t a n e o u s tissue u n d e r n e a t h treated skin - multiple, red
areas, up to 1.2 x 0.8 cm; treated skin - thickened.
F20465
F
-- 14
Subcutaneous skin underneath treated skin - multiple, red, p inpo in t foe i .
F20485
F
14 G020
Subcutaneous skin underneath treated skin - multiple, red
a r e a s , up to 0.2 x 0.1 cm.
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. P.O. BOX 7545 MADISON, W ISCONSIN 53707 PH O N E (608) 241-4471 TLX 703956 H AZR AL M DS UD
SAMPLE NUMBER: 70905762 SA MP LE : T-41.02
PAGE
8
OECD DERMAL SCREEN
(CONTINUED)
Re fe r e n c e s :
1. O r g a n i s a t i o n f o r E c o n o m i c C o o p e r a t i o n a n d D e v e l o p m e n t ' s G u i d e l i n e s for Testing of C h e m i c a l s , Section 402, Acute Dermal Toxicity, Ad op t e d hay 12, 1981.
2. D r g i z e , 3 . H . , " A p p r a i s a l of the S a f e t y of C h e m i c a l s in F o o d s , D r u g s , and Cosmetics - Dermal Toxicity", Association of Food and Drug
Officials of the U.S., p p . 46-59 (1975).
3. O E C D P r i n c i p l e s of G o o d L a b o r a t o r y P r a c t i c e , A n n e x 2, C C 8 1 ) 3 0 ( F in al ).
4. N I H P u b l i c a t i o n No. 8 5 - 2 3 ( r e v i s e d 19 85 ).
000321
HLA LA NO. 70905762 PATHOLOGY REPORT
Acute Dermal Toxicity
Page 9
Ten rabbits (five males, five females) were necropsied. One male died on test
and the remaining animals were euthanatized at the termination of the study. The dose level, day of death, and gross observations recorded for each animal
are on page 7 of this report. The subcutaneous tissue of the treated skin of four females had multiple red foci or areas of variable size. The treated skin of one of these appeared to be thickened. Several animals were noted to be tnin. All otner observations were considered incidental and unrelated to treatment. The treated skin of the died on test male was diffusely red. This animal's death was attributed to enteritis of unknown etiology and unrelated to treatment.
Pathologist ( 1734mcs)
Date
GG0522
BEST COPY AVAILABLE
Personnel Signature Sheet Acute Toxicology
HLA No. 7 0 R O S 7 ( o L
Name
Job Title
Signature
Becky Beckwith
Sr. Lab. Animal Asst.
Steve Beloungy
Lab. Animal Asst.
Ken Bridges Pat Crary
Sr. Lab. Animal Asst. Sr. Lab. Animal Asst.
Shane E1th
Lab Animal Asst.
Shawn Frazier
Lab Animal Asst.
Steven M. Slaza
6roup Leader
Nolly Hahn
Admin. Clerk
Kevin Haul1ton
Lab. Animal Asst.
Jeff Hicks
Sr. Lab. Animal Asst.
Calvin Horton
Group Leader
Sharon L. Howery Administrative Asst.
Gregory Johnson
Lab. Animal Tech.
Paul Krebs
Lab. Animal Asst.
Meyne Nadlson
Section Supervisor
Scott NcConnell
Lab. Animal Tech.
Shelley NcConnell Dawn Conant
Lab. Animal Tech. Sr. Lab. Animal Asst.
Don Nevis
Lab. Animal Asst.
Robin Olson
Lab. Animal Asst.
Patricia Padgham Joseph J. Daun
Team Leader Lab. Animal Asst.
Nlchael Patzka
Lab. Animal Asst.
John Paulson
Sr. Lab.' Animal Asst.
Jane Polnow
Lab. Animal Tech.
Dennis B. Steiner Lab. Animal Tech.
Nlchael Theslng
Lab. Animal Asst.
Paula 6. Vangen
Administrative Asst.
Albert Olson
Manpower
Jim Jirschele
LTE
Ben Haley
Sr. Lab Animal Asst.
Eileen M. McConnell Admin. Clerk
Annette R. Turner Manpower
j& M L J m s
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Initials
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-
000523
REST COPY AVAILABLE
HLA; 10^0Sl(o9-
ACUTE DERMAL APPLICATION LD50 IN NEW ZEALAND WHITE RABBITS
Test Material: -j- MI QSl
Physical Description: __rtflrW &n)n>v
Vehicle: Jd ,
Dosage Level (g/kg): r?-Qq/l<3 ate Anliittnal:s Clip.,ped:/__;_t_e_/_g_?_______ Technician: 6 f t
Date Animal Received: wI/P^ l#e i
Source: Hazleton Research Products
Room No.: -3<=w
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c. <`/W*7- "/
Animal Number
DOSE CALCULATIONS
COMPOUND PREPARATION WEIGHTS
E t a b T o lidi
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Dose
Tare Weight Total Weight Sample Weignt
tko)
(sAg) Animal (g)
()
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Scale Used:
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Date: //-/ -S? Conducted by:
& D Date:
7 Approved by:
T<O-
to>o<f P il\
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Date: Date:
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Animal Body Weionts (g)
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Prep
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CofZec-'e*/ /&ft -/if
Reviewed By Q o . (00261/vmt)
Date.i Q l
BEST COPY AVAILABLE
MORTALITY REORO
HLA: l Q Q 6 5 l ( o ^
Test material:
*j/0 L
Dose level:
i 2Animal
3
4 5 6 e 9 10Observation Period (Days) i
11
12 13 14
Number
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Reviewed by:
Date: I Q-fa f f f
(00261/vmt)
Dose level:
O O O Cfi 10
cri
BEST COPY AVAILABLE
OBSERVATIONS (Individual)
Test material: "f-tJ lO c .
HLA: 7^703 7 &A
UFV^.-O Ttt/Wu,fifc|i3>U<b Au&nrtAS 1
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Surviving animals submitted for terminal necropsy.
Technician { f t
Date U/W;a a
Surviving animals
designated for sacrifice
and discard.
Tpchnlclan
fOfl_______
Date
Oft
<be+*s*r*rm hit?
Oste, u d *
a ***
^
v Indicates condition exists
SI Slight. - Condition not evident. * Found dead, P.M. check.
Qr)Reviewed by:
Date: Ic ia & j
Dose level:
IFST COPY AVAILABLE
OBSERVATIONS (Individual) Test material: J7~- V/<03.
HLA: -7fl?<3iS7.'n?
"JEST COPY AVAILABLE
HU: IM O S lte
ACUTE DERMAL IRRITATION OBSERVATIONS
Test material: 'T ~
Dose level: A
mi
Ervthema Edema Atonla Desauamatlon Coriaceousness F1ssur1no
Ervthema Edema Atonla Desauamatlon Coriaceousness F1ssur1no
Ervthema Edema Atonla Desauamatlon Coriaceousness F1ssur1nq
1
hi
Anima
i 0 O 0 0 n
Observation Period (Davsl
Males
Females
3
10 14 '
3 7 10 14
V>q ''33
li5 i
No.: P2-64SSTntacfyAbraded
/ 1 -- f--
i
/J /
i
On
d?
A
0 r>
Ci
oa
a
0 a 3
r\
" / n ' U _ "h i*
Anima No. :P2-cs3 Intact!/Abraded
1
/ ) ~~r
i
t ./
1
1
o
oA
f) _ Ci
n Ao
o
__ D __
__ n
a
Anima
1
0 0
n n
Anima n 0 n
o
O o
No. :F2-rt441 Ofntac^Abraded
/ 1m i
J
/ n
A
D 0D
0 3(% v
0 o
O \I
t?`hit/fl
Mn rfntact/Abraded
oa
0
0
oo
0
o
oG
0
n
r> t
i
o n0
o
h _ C ____ n
Animal No.: F Z - ^ d i t a c ^ A braded
1
/1
r_ O _
n / rs 0 O
n O r> W t l T ~ Q
o 0 r> A tLl 1
r> A 0 A
n n rs 1
Animal No.: Fa-cs-jTtf'ntcVA braded
1
(i
7 O
o on o O
n Gn n o
O n tro o
n
O fS Q
o
o. A
6
CA
Ervthema Edema Atonla Desauamatlon Coriaceousness F1ssur1na
Anima
U
6
r> o n
a
No. :PZ-W^(ntacJ>Abraded o
o n/si ft"7 ef O O
0
Animal No.: F7_-olfc4 Lniftct^Abraded
J SL 5
1
/ /i
A
fi
oo
0
n o 0 1A
n
b fi
nA
_ _____ O
___ ____ A ___
Ervthema
Edema Atonla Desauamatlon
Coriaceousness F1ssur1na
Technician
Date
iqg?
Anima
1 0
n n n
Q
No. :F2-<0SlW<;Tntact7ftbraded
1 1>145 i
I
0 rt 0
a
n0
1
o /S 1 t
1
o ri 0
e> a
i
1
& kR Qj<(.5 J k - J 1 _ 1& 3 ..
ttft 7v T
Animal No. :fi-C ti& T ntacOAbraded
1/
A
n O f) 0 O
A
o f*) JUL <?
O
0o
rWTT
n o
O
o
0 ri
nO
as L S v au),
-- UK.
>'/6 ci
A - Subcutaneous hemorrhage. B - Blanching. C - Scab formation. D - Eschar. E - Exfoliation.
>
fOO?Sl/vmn
6) /Jr*V fa.s < 6
<S><iJCOrXi, ScXrC.ES E/oViZeQ . * 8 "/{g-*.
W o * )$<*>)
-<27
Reviewed by:^ k n __________ Date: -fa-&7
000528
