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3M Company St. Paul, Minnesota Tennessee River Perfluorochemical (PFC) Assessment Fish Tissue and Surface Water Sampling Report October - December 2012 3M Decatur, Alabama June 2013 11P-0094-9 TENNESSEE RIVER PERFLUOROCHEMICAL (PFC) ASSESSMENT FISH TISSUE AND SURFACE WATER SAMPLING REPORT OCTOBER - DECEMBER 2012 JUNE 2013 Prepared for 3M Company St. Paul, MN 55144 by WESTON SOLUTIONS, INC. West Chester, PA 19380 W.O. No. 02181.129.134 TABLE OF CONTENTS Section Page E.S. EXECUTIVE SUMMARY......................................................................................... ES-1 1. INTRODUCTION.......................................................................................................... 1-1 2. MATERIALS AND METHODS.................................................................................. 2-1 2.1 FISH SA M PLIN G ........................................................................................................2-1 2.2 SURFACE WATER SAMPLING..............................................................................2-6 2.3 STATISTICAL A N A LY SIS...................................................................................... 2-9 3. RESULTS....................................................................................................................... 3-1 3.1 FISH ................................................................................................................................3-1 3.1.1 Largemouth B a s s ..........................................................................................3-1 3.1.2 Channel Catfish.............................................................................................3-2 3.2 SURFACE WATER SAMPLING R E SU L T S.........................................................3-2 3.2.1 3.2.2 Surface W ater at Fish Sampling R eaches................................................. 3-2 Surface W ater at Transect Locations.........................................................3-3 3.3 QUALITY ASSURANCE AND QUALITY CONTROL...................................... 3-3 4. DATA EVALUATION.................................................................................................. 4-1 4.1 EVALUATION OF FISH SAMPLING R ESU LTS................................................ 4-2 4.1.1 4.1.2 4.1.3 Historical D a ta ..............................................................................................4-2 Graphical Exam ination................................................................................4-5 Statistical Analysis....................................................................................... 4-6 4.2 EVALUATION OF SURFACE WATER SAMPLING R ESU L T S...................4-11 4.2.1 4.2.2 4.2.3 Historical D a ta ............................................................................................4-11 Graphical Exam ination..............................................................................4-13 Statistical Analysis..................................................................................... 4-15 5. SUMMARY OF KEY FINDINGS............................................................................... 5-1 5.1 TEMPORAL TRENDS IN FISH FILET TISSUE CONCENTRATIONS.........5-1 5.2 SPATIAL EVALUATION OF FISH FILET TISSUE PFOS CONCENTRATIONS................................................................................................. 5-2 5.3 EVALUATION OF SURFACE WATER PFOS CONCENTRATIONS...........5-3 6. REFERENCES............................................................................................................... 6-1 J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface W ater Report.doc i APPENDICES APPENDIX A INTERLABORATORY COMPARISON APPENDIX B SUMMARY OF PERFLUOROCHEMICAL ANALYTICAL RESULTS APPENDIX C 3M ENVIRONMENTAL LABORATORY ANALYTICAL DATA PACKAGES APPENDIX D AXYS ANALYTICAL SERVICES ANALYTICAL DATA PACKAGE APPENDIX E WATER QUALITY PARAMETER DATA TABLES APPENDIX F STATISTICAL OUTPUTS (Provided on CD) J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface W ater Report.doc 11 LIST OF FIGURES Figure ES-1 Fall 2012 Fish Filet Tissue Mean Median and Geometric Mean - PFOS Concentration Figure 2-1 Fall 2012 Fish Sampling Reaches and Surface W ater Sampling Locations Figure 2-2 Example Box Plot Figure 3-1 Fall 2012 Fish Filet Tissue Results - PFOS Concentrations Figure 3-2 Fall 2012 Fish Filet Tissue Mean Median and Geometric Mean - PFOS Concentration Figure 3-3 Largemouth Bass Filet Median PFOS Concentrations - Fall 2012 Figure 3-4 Channel Catfish Filet Median PFOS Concentrations - Fall 2012 Figure 3-5 Fall 2012 Surface Water - Fish Reaches PFOS Concentrations Figure 3-6 Fall 2012 Surface Water Average Transect Locations - PFOS Concentrations Figure 4-1 Largemouth Bass PFOS Box Plots for the River Mile 320 (Reach 01) Below Triana, AL Location 2007 to 2012 Figure 4-2 Largemouth Bass PFOS Box Plots for the River Mile 307.5 (Reach 02) Point Mallard Location 2004 to 2012 Figure 4-3 Largemouth Bass PFOS Box Plots for the River Mile 301 (Reach 05) Bakers Creek Mouth Location 2004 to 2012 Figure 4-4 Largemouth Bass PFOS Box Plots for the River Mile 296 (Reach 09) Fox Creek Mouth Location 2002 to 2012 Figure 4-5 Largemouth Bass PFOS Box Plots for the River Mile 293.5 (Reach 11) Mallard Creek Mouth Location 2004 to 2012 Figure 4-6 Largemouth Bass PFOS Box Plots for the River Mile 281 (Reach 13) Location 2007 to 2012 Figure 4-7 Largemouth Bass PFOS Box Plots for the 2004 Sampling Locations Figure 4-8 Largemouth Bass PFOS Box Plots for the 2006 Sampling Locations Figure 4-9 Largemouth Bass PFOS Box Plots for the 2012 Sampling Locations J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface W ater Report.doc iii LIST OF FIGURES (CONTINUED) Figure 4-10 Channel Catfish PFOS Box Plots for the River Mile 320 (Reach 01) Below Triana, AL Location 2007 to 2012 Figure 4-11 Channel Catfish PFOS Box Plots for the River Mile 307.5 (Reach 02) Point Mallard Location 2002 to 2012 Figure 4-12 Channel Catfish PFOS Box Plots for the River Mile 301 (Reach 05) Bakers Creek Mouth Location 2004 to 2012 Figure 4-13 Channel Catfish PFOS Box Plots for the River Mile 296 (Reach 09) Fox Creek Mouth Location 2002 to 2012 Figure 4-14 Channel Catfish PFOS Box Plots for the River Mile 293.5 (Reach 11) Mallard Creek Mouth Location 2004 to 2012 Figure 4-15 Channel Catfish Box Plots for the River Mile 281 (Reach 13) Location 2007 to 2012 Figure 4-16 Channel Catfish PFOS Box Plots for the 2004 Sampling Location Figure 4-17 Channel Catfish PFOS Box Plots for the 2006 Sampling Locations Figure 4-18 Channel Catfish PFOS Box Plots for the 2012 Sampling Locations Figure 4-19 Surface Water Temporal Evaluation at River Mile 307.5, River Mile 301 and River Mile 296 Figure 4-20 Surface Water Temporal Evaluation at River Mile 307.5, River Mile 301 and River Mile 296 [Adjusted Scale] Figure 4-21 Average PFOS Concentration at Fish Reach Sampling Locations - Fall 2012 Figure 4-22 Average PFOS Concentrations at Transect Sampling Locations - Fall 2012 J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface W ater Report.doc iv LIST OF TABLES Table 3-1 Fish Sampling Summary and Catch Per Unit Effort Table 3-2 Largemouth Bass Fish Morphometries and PFC Concentrations Table 3-3 Channel Catfish Fish Morphometries and PFC Concentrations Table 3-4 Largemouth Bass Filet PFOS Concentration Summary Statistics Table 3-5 Channel Catfish Filet PFOS Concentration Summary Statistics Table 3-6 Surface W ater PFC Data - Fish Reach Locations Table 3-7 Surface W ater PFC Data - Transect Locations Table 3-8 Interlaboratory Analytical Comparison - PFOS Table 4-1 Mann-Kendall Test for Trend in Fish Filet Tissue PFOS Concentration Table 4-2 Mann-Whitney Test for Significant Differences in Median PFOS Concentrations in Largemouth Bass Filet Tissue (Temporal Evaluation) Table 4-3 Mann-Whitney Test for Significant Differences in Median PFOS Concentrations in Channel Catfish Filet Tissue (Temporal Evaluation) Table 4-4 Mann-Whitney Test for Significant Differences in Median PFOS Concentrations in Largemouth Bass Filet Tissue (Spatial Evaluation) Table 4-5 Mann-Whitney Test for Significant Differences in Median PFOS Concentrations in Channel Catfish Filet Tissue (Spatial Evaluation) Table 4-6 Mann-Kendall Test for Trend in Surface Water PFOS Concentrations J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface W ater Report.doc v 3M ADEM ADPH AXYS CPUE DCNR FBSA FOSA GPS HPLC M S/M S ng/g ng/L ng/mL NIST NPDES PFBS PFC PFHpA PFHS PFHxA PFOA PFOS ppb ppt Q A /Q C RM RPD SCP SRM WESTON LIST OF ACRONYMS 3M Company Alabama Department o f Environmental M anagement Alabama Department o f Public Health AXYS Analytical Services, Ltd. Laboratory catch per unit effort Department of Conservation and Natural Resources perfluorobutanesulfonam ide perfluorooctanesulfonam ide global positioning system high performance liquid chromatography tandem mass spectrometry nanograms per gram (ppb) nanograms per liter (ppt) nanograms per milliliter (ppb) National Institute o f Standards and Technology National Pollutant Discharge Elim ination System perfluorobutanesulfonate perfluorochem ical perfluoroheptanoic acid perfluorohexanesulfonate perfluorohexanoic acid perfluorooctanoic acid perfluorooctane sulfonate parts per billion parts per trillion quality assurance/quality control River Mile relative percent difference scientific collection permit standard reference material W eston Solutions, Inc. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface W ater Report.doc vi EXECUTIVE SUMMARY Since 2000, the 3M Company (3M) has cooperated with the Alabama Department of Environmental Management (ADEM) and the Alabama Department of Public Health (ADPH) to assess perfluorochemical (PFC) levels in fish and surface water in the Tennessee River. Eight sampling events for surface water and eight sampling events for fish tissue have been performed for perfluorooctane sulfonate (PFOS) in the vicinity of the 3M Decatur facility. All of these sampling activities were executed with the knowledge and/or involvement of ADEM and/or ADPH. In the fall of 2012, the most comprehensive fish and surface water PFC data set developed to date in the study area was collected. The fall 2012 sampling spanned an approximately 56 river mile portion of the Tennessee River from River Mile (RM) 320 near Triana, AL downstream to RM264 below Florence, AL. This sampling was performed between October 31, 2012 and December 5, 2012, by Weston Solutions, Inc. (WESTON), on behalf of 3M. Surface water sampling and fish collections were performed at 13 reaches in the W heeler Reservoir and two reaches in the Wilson Reservoir of the Tennessee River. A Work Plan, reviewed and approved by ADEM and ADPH prior to the initiation of field activities, called for the collection of five largemouth bass (Micropterus salmoides) and five channel catfish (Ictalurus punctatus) at each of 15 sampling reaches, for a total of 150 fish (WESTON, 2012a). Fish sampling was performed in accordance with a scientific collection permit (SCP) issued by the Alabama Department of Conservation and Natural Resources (DCNR) to WESTON, and all targeted samples were collected. The Work Plan also called for the collection of three surface water samples from each fish sampling reach as well as ten surface water samples at each of nine pre-determined surface water transects across the river. All proposed surface water samples were collected in accordance with the Work Plan. This sampling was performed primarily to characterize the current concentrations of PFOS in the 150 fish filet samples, 135 surface water samples and associated sample duplicates and other quality assurance/quality control (QA/QC) samples. This emphasis J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc ES-1 on PFOS results from its potential to bioaccumulate and its identification as a contaminant of emerging concern. The PFOS concentration findings from this study are also discussed in the context of historical information. In addition, several other PFCs that are subject to monitoring in National Pollutant Discharge Elimination System (NPDES) permits issued by ADEM were included as target analytes (i.e., perfluorobutanoic acid (PFBA), perfluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA), perfluorobutanesulfonate (PFBS), perfluorohexanesulfonate (PFHS), perfluorobutanesulfonamide (FBSA), perfluorooctanesulfonamide (FOSA), 2-(N-methyl-PFOSA) acetic acid (N-MeFOSAA), and 2-(N-ethyl-PFOSA) acetic acid (N-EtFOSAA)). All primary fish and surface water samples, as well as duplicates at a 10% duplicate to primary ratio, were analyzed by the 3M Environmental Laboratory in St. Paul, MN. An additional subset of samples, at a 10% duplicate to primary ratio, were collected and submitted by WESTON to AXYS Analytical Services, Ltd. Laboratory (AXYS) in Sidney, British Columbia, Canada for verification. Interlab oratory and intralaboratory comparisons indicated very good agreement between PFC analytical results and reinforce the validity of the results. PFOS was detected in all fish samples collected and median, mean and geometric mean concentrations, by species, are presented in Figure ES-1. Median largemouth bass PFOS concentrations ranged from 25.7 to 435 nanograms per gram (ng/g) (ppb), and median channel catfish PFOS concentrations ranged from 1.50 to 283 ng/g (ppb) over the study area. Consistent with previous studies in this section of the Tennessee River, PFOS concentrations were consistently lower in channel catfish compared to largemouth bass. PFOS concentrations were statistically significantly higher in fish collected along the southern shoreline in the industrialized area of Decatur than in the upstream sampling locations and at the cross river northern shoreline locations. Furthermore, median PFOS concentrations were significantly lower in the farthest downstream locations relative to the median PFOS concentrations within the industrialized area of Decatur. Relative to the median PFOS concentrations at their respective adjacent upstream locations, the first downstream instances of statistically significantly lower median PFOS concentrations J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc ES-2 along the southern shoreline below Decatur were observed for channel catfish at the Fox Creek mouth location (RM296) and for largemouth bass at the Mallard Creek mouth location (RM293.5). Compared to historical data sets, statistically significant decreasing trends in largemouth bass filet PFOS concentrations were identified by Mann-Kendall analysis at all locations tested within or downstream of the industrialized area of Decatur (RM281 below Spring Creek, Fox Creek mouth and Bakers Creek mouth locations). Mann-Whitney testing identified statistically significant reductions in median largemouth bass PFOS concentrations over the period of record for all but the Swan Creek mouth and Mallard Creek mouth locations. However, the Mallard Creek mouth median largemouth bass PFOS concentration was statistically significantly lower in 2012 than the median from the previous sampling in 2006. The Swan Creek mouth PFOS tissue concentrations have been lower than those along the southern shoreline over the period of record. For channel catfish, Mann-Kendall analysis identified a statistically significant decreasing trend in PFOS concentrations over time at the Bakers Creek mouth location and Mann-Whitney tests indicated statistically significant reductions in median channel catfish PFOS concentrations at the Bakers Creek mouth and Fox Creek mouth locations within the immediate vicinity of the industrialized area of Decatur. For the overall study area, surface water PFOS concentrations ranged from 0.00639 to 0.385 nanograms per milliliter (ng/mL; parts per billion [ppb]) throughout the fish sampling reaches and from 0.00611 to 0.138 ng/mL in samples from the surface water transects. The highest surface water PFOS concentrations were observed at sampling locations along the industrialized southern shoreline in the immediate vicinity of Decatur. The PFOS data across the river indicate that slow mixing and bank attachment of plumes occurs along the southern shoreline. The transect data indicate that complete mixing across the river occurs between Mallard Creek mouth at RM293.5 and below Spring Creek at RM281. Similar to the fish results, Mann-Kendall testing identified statistically significant decreasing trends in surface water PFOS concentrations at the Fox Creek mouth and J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc ES-3 Bakers Creek mouth locations within the immediate vicinity of the industrialized area of Decatur. Additional findings and detailed spatial and temporal evaluations of PFOS concentrations in the Tennessee River are included in this report. Data for the additional PFCs analyzed during the fall 2012 fish and surface water sampling, along with details pertaining to the sampling and analysis performed, are also provided in this report. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc ES-4 Reach 15 S ta tis tic Bass M edian 86.9 Mean 84.9 Geo Mean 83.8 Catfish 7 .3 3 7 .9 5 7 .7 4 Reach 12 S ta tis tic Bass M edian 6 5 .8 Mean 70.1 Geo Mean 6 8 .8 Catfish 10.7 8.71 8 .1 0 Reach 14 S ta tis tic Bass M edian 75.3 Mean 1 r ' o r IV /I n 7 3 .3 7-1 A Catfish 8.01 9 .1 2 o a r\ Reach 13 S ta tis tic Bass M edian 7 1 .0 Mean 77.0 Geo Mean 7 6 .2 Catfish 10.3 9 .7 7 9 .6 6 R e a c h 11 S ta tis tic Bass M edian 145 Mean 162 Catfish 15.7 15.3 Reach 10 S ta tis tic Bass M edian 104 Mean 134 Geo Mean 122 Catfish 13.4 12.2 11.8 Reach 08 S ta tis tic Bass M edian 8 0 .3 Mean 102 Geo Mean 9 1 .8 Catfish 4 .8 9 8 .3 8 5 .9 0 Round Island I Creek F.Svvan C reek I Reach 06 S ta tis tic Bass M edian 5 0 .6 Mean 6 5 .5 Geo Mean 6 0 .4 Catfish 4 .1 5 3.70 3.59 ' * Legend Facility Boundary | _ _ | Fish Sampling Reaches Notes: -Mean = Arithmetic Mean -Geo Mean = Geometric Mean -Only primary sample results were used for these statistical calculations Reach 09 S ta tis tic Bass M edian 259 Mean 232 Geo Mean 226 Catfish 18.6 2 2 .5 2 0 .0 Reach 07 S ta tis tic Bass M edian 262 Mean 286 Geo Mean 275 Catfish 2 9 .2 4 0 .3 3 3 .3 Reach 05 S ta tis tic Bass M edian 435 Mean 405 Geo Mean 345 l4 Catfish 283 256 199 | Bakers Creek | |r r a I Betty Rye & I Dry Branch I Reach 04 S ta tis tic Bass M edian 159 Mean 208 Geo Mean 181 Catfish 7 .8 5 19.0 10.6 ".IK .'/ Reach 03 S ta tis tic Bass M edian 2 5 .7 Mean 3 5 .2 Geo Mean 31.4 Catfish 2.01 2 .0 9 1.75 Reach 02 S ta tis tic Bass M edian 44.1 Mean 4 3 .2 Geo Mean 4 2 .7 Catfish 2 .6 5 2.41 2 .2 8 R e a c h 01 S ta tis tic Bass M edian 3 2 .7 Mean 3 0 .6 Geo Mean 2 9 .7 Catfish 1.50 2 .0 2 1.55 Imagery Source: ESRI Bing Mapping Service 2011 N W^ E 0 12 4 I Miles Figure ES-1 Fall 2012 Fish Filet Tissue Mean Median and Geometric Mean PFOS Concentrations Tennessee River 1. INTRODUCTION Fish sampling was performed by Weston Solutions, Inc. (WESTON) for the 3M Company (3M) between October 31, 2012 and November 29, 2012 at 13 reaches in the Wheeler Reservoir and two reaches in the Wilson Reservoir of the Tennessee River. Surface water samples were collected at each fish collection reach between November 16 and 20, 2012, as well as across nine surface water transects spanning the study area between December 3 and 5, 2012. The sampling spanned an approximately 56 river mile portion of the Tennessee River from River Mile (RM) 320 near Triana, AL, downstream to RM264 above Florence, AL. The sampling was performed by WESTON for 3M as part of 3M 's continuing cooperative effort with the Alabama Department of Environmental Management (ADEM) and the Alabama Department of Public Health (ADPH) to assess perfluorochemical (PFC) levels in fish in the Tennessee River. This sampling was performed to characterize current concentrations of perfluorooctane sulfonate (PFOS) in filet samples from largemouth bass (Micropterus salmoides) and channel catfish (Ictaluruspunctatus). This emphasis on PFOS results from its potential to bioaccumulate and its identification as a contaminant of emerging concern. In addition, several other PFCs that are subject to monitoring in National Pollutant Discharge Elimination System (NPDES) permits issued by ADEM were included as target analytes (i.e., perfluorobutanoic acid (PFBA), perfluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA), perfluorobutanesulfonate (PFBS), perfluorohexanesulfonate (PFHS), perfluorobutanesulfonamide (FBSA), perfluorooctanesulfonamide (FOSA), 2-(N-methyl-PFOSA) acetic acid (N-MeFOSAA) and 2-(N-ethyl-PFOSA) acetic acid (N-EtFOSAA)). This report includes detailed information regarding the performance of the fall 2012 fish and surface water study. Because o f the primary interest in PFOS, the body of this report is written to present detailed information on this analyte. The corresponding results for the additional constituents are also presented. A discussion of the findings from this study as a whole, as well as in the context of historical information, is also provided. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 1-1 This document is organized into the following sections: Section 1 - Introduction. This section is an overview of the fall 2012 fish and surface water sampling and includes descriptions of the contents of this report. Section 2 - Methods and Materials. This section contains details of the fall 2012 sample collection activities, including descriptions of the sampling locations, sampling methods and sample preparation procedures, the numbers and types of samples collected, analytical methods and the quality assurance/quality control (QA/QC) provisions. Section 3 - Results. This section includes the results of the laboratory analyses of PFOS concentrations in the surface water and fish filet tissue samples collected during the fall 2012 sampling event. Section 4 - Data Evaluation. In this section, the PFOS results from the fall 2012 sampling event are discussed in the context of historical data, and the spatial and temporal trends are evaluated. Section 5 - Key Findings. In this section, the key findings from the fall 2012 sampling event and historical evaluation are presented. Section 6 - References. This section provides a listing of the literature cited. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report'Draft Final Fall 2012 Fish and Surface Water Report.doc 1-2 2. MATERIALS AND METHODS The study area, sampling locations, sampling methods and sample preparation procedures, numbers and types of samples collected, analytical methods and QA/QC procedures for the fall 2012 fish and surface water study are described in the following subsections. 2.1 FISH SAMPLING Fish sampling was performed in accordance with a scientific collection permit (SCP) issued by the Alabama Department of Conservation and Natural Resources (DCNR) to WESTON. All sampling was performed in accordance with a work plan (WESTON, 2012a) that had been reviewed and approved by ADEM and ADPH prior to the initiation of field activities. Observation of electrofishing and trotlining was performed by ADEM personnel on several occasions during fish collection activities. Individual fish sampling reaches (extending from upstream to downstream) are illustrated in Figure 2-1 and are summarized as follows: 1. Tennessee River RM320 - Sampling reach located below Triana, AL, at RM320. Previously sampled by ADEM as TENR-320 in 2007, 2008 and 2009. 2. Point Mallard - Sampling reach located on the southern shoreline of the main channel near Point Mallard above the City of Decatur at approximately RM307.5. Previously sampled by 3M as LOC-3 (DL3) in 2004 and 2006. 3. Tennessee River RM307.5 - Sampling reach located in the backwater area to the north of the main channel near Point Mallard above the City o f Decatur at approximately RM307.5. 4. Mouth of Betty Rye Creek and Dry Branch - Sampling reach located at the confluence of Betty Rye Creek and Dry Branch with the Tennessee River. Sampling reach is along the southern shoreline o f the Tennessee River at approximately RM303. 5. Bakers Creek Mouth - Sampling reach located at the confluence o f Bakers Creek with the Tennessee River at approximately RM301. The sampling reach extends along the southern shoreline of the Tennessee River. Previously sampled by 3M J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 2-1 as DBC in 2004, 2006 and spring 2012. Previously sampled by ADEM as WHEL11 in 2007, 2008 and 2009. 6. Swan Creek Mouth - Sampling reach along the northern shoreline of the Tennessee River at the mouth of Swan Creek at approximately RM301.2. Previously sampled by 3M as LOC-2 (DL2) in 2004 and 2006. 7. Tennessee River RM298 - Sampling reach along the southern shoreline of the Tennessee River at approximately RM298. Sampling reach is upstream of the confluence of Fox Creek with the Tennessee River. 8. Round Island Creek Mouth - Sampling reach along the northern shoreline of the Tennessee River at the mouth of Round Island Creek at approximately RM298. 9. Fox Creek Mouth - Sampling reach along the southern shoreline of the Tennessee River at the mouth of Fox Creek at RM296. Previously sampled by 3M as LOC-1 (DL1) in 2004 and 2006. 10. Browns Ferry - Sampling reach along the northern shoreline of the Tennessee River in the vicinity of Browns Ferry at approximately RM294. 11. Mallard Creek Mouth - Sampling reach along the southern shoreline of the Tennessee River at the mouth of Mallard Creek at approximately RM293.5. Previously sampled by 3M as DMC in 2004 and 2006. 12. Elk River Mouth - Sampling reach along the northern shoreline of the Tennessee River at mouth of the Elk River at approximately RM285. 13. Tennessee River RM281 - Sampling reach along the southern shoreline of the Tennessee River below the mouth of Spring Creek at RM281 and above the Joe Wheeler Dam. Previously sampled by ADEM as TENR-281 in 2007, 2008 and 2009. 14. Tennessee River RM273 - Sampling reach along the southern shoreline of the Tennessee River in the Wilson Reservoir below the mouth of Big Nance Creek at RM273. 15. Tennessee River RM264 - Sampling reach along the northern shoreline of the Tennessee River in the Wilson Reservoir below the mouth of Shoal Creek at RM264. As depicted in Figure 2-1, these 15 sampling reaches provide an extensive representation of the Wheeler Reservoir, including northern and southern shorelines both upstream and downstream of heavily industrialized areas in the vicinity of Decatur, AL, as well as a limited assessment of the northern and southern shorelines of the Tennessee River downstream of the Joe Wheeler Dam, within the Wilson Reservoir. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 2-2 The target species for fish sampling were the largemouth bass and channel catfish. These species were selected for the following reasons: Consistency with the fish samples collected in earlier sampling performed by 3M in 2004, 2006 and 2012 and collections by ADEM in 2007, 2008 and 2009. Predator species representing the highest trophic level. Bottom-dwelling species with possible greater potential for exposure to sediment- associated constituents (channel catfish). Species with a wide distribution and commonly consumed. Recommended target species (EPA, 1993; EPA 2000). Electrofishing and trotlining methods were employed to collect fish samples. Electrofishing, the primary gear type for the collection of largemouth bass, was conducted using a boat-mounted electrofishing unit consisting of generator-powered Smith-Root VI-A electrofisher fitted with two bow-mounted anodes and a single trailing cathode. In each fish sampling reach, electrofishing focused on shoreline areas where sufficient structure was present to attract and hold largemouth bass. The primary method for collecting catfish was trotlining consisting of long lengths of #36 tarred seine twine with multiple snelled hooks in sizes No. 2 through No. 8 attached by trotline clips and swivels. Trotlines were primarily baited with whole threadfin shad (Dorosoma petenense) and, occasionally, with cut bait obtained from gizzard shad (D. cepedianum). Bait fish were obtained by electrofishing and cast netting. Typical individual trotline sets were approximately 150 to 250 feet in length with 40 to 50+ baited hooks equidistantly spaced between anchors and float lines. Both daytime and overnight sets were made. Daytime sets were tended periodically to collect target fish, release non-target species such as blue catfish (I. furcatus) and re-bait. The target size range for largemouth bass was 13 to 18 inches in length, and the target size range for channel catfish was 14 to 24 inches in length, coinciding with the target sizes listed in the ADEM Fish Tissue Monitoring Program Sample Collection Procedures (SOP #2300 Rev. 2.0; ADEM, 2012). The target sample size for both largemouth bass and channel catfish from each of the 15 fish sampling reaches was five specimens of each species for a total of 75 largemouth bass and 75 channel catfish. All target samples were attained. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report'Draft Final Fall 2012 Fish and Surface Water Report.doc 2-3 Additional effort was required in some areas with marginal bass habitat and/or lower species abundance relative to non-target species (i.e., channel and blue catfish in Wilson Reservoir). Sufficient quantities of target fish were present in most of the sampling reaches so that their pre-established locations did not require adjustment by relocation or expansion in the field. Minor alterations were made in the sizes and locations o f some fish sampling reaches to incorporate shoreline areas with suitable bass habitat and abundance. Figure 2-1 depicts the extent of each reach as sampled. In order to collect data suitable for assessing the range of variability in fish tissue PFC concentrations and for consistency with past 3M and ADEM collections, samples were prepared from individual fish specimens for analysis rather than composite samples from multiple specimens. Any deformities, erosions, lesions or tumors (DELTs) were noted, and the data were recorded in the field logbook. Skin-off filet tissue samples were prepared in the field from the right side filets of the fish specimens (primary samples). Fish samples were held on wet ice prior to fileting within approximately 48 hours of capture. Filet samples were weighed, packaged in two polyethylene ziploc bags with sample ID information recorded on a label placed between the two bags, and stored in a freezer prior to shipment. Fish tissue samples were shipped on dry ice to the TestAmerica Burlington laboratory for sample homogenization and reshipment on dry ice to the 3M Environmental Laboratory for perfluorochemical analyses. Verification samples were sent to the AXYS Analytical Services, Ltd. laboratory (AXYS) in Sidney, British Columbia, Canada, for perfluorochemical analyses in accordance with the Work Plan. In addition to the primary samples, field duplicate samples and QA/QC samples were prepared. Field duplicate samples were prepared at a minimum of 10% duplicate to primary sample ratio from the left side filets of the chosen fish specimens. A total of 30 duplicate skin-off filet samples were prepared with 15 duplicate samples designated for analysis by the 3M Environmental Laboratory in St. Paul, MN, and 15 duplicate samples designated for analysis by AXYS. Primary sample results from 3M Environmental Laboratory analyses are tabulated and used in statistical analyses in this report. Analytical results from field duplicates (left side J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report'Draft Final Fall 2012 Fish and Surface Water Report.doc 2-4 filets) analyzed by the 3M Environmental Laboratory and the AXYS laboratory were used for verification of analytical accuracy and performance. A detailed interlaboratory comparison of duplicate sample results and a letter from ADEM to 3M acknowledging the good agreement between laboratories and reinforcing the validity of the results of this study are included in Appendix A. A summary table including the primary and duplicate PFC results is included in Appendix B. On the basis intralaboratory and interlaboratory comparisons and the results from other QA/QC samples, the study analytical results are valid and meet the data quality objectives. Primary sample results reported by the 3M Environmental Laboratory were based on the analysis of two aliquots from the primary sample volume. The results were reduced to a single value by calculating the average concentration (arithmetic mean) for sets comprised of numeric values. In instances with mixed numeric values and non-numeric values (non-detects), the numeric values were carried through to represent the media concentrations. In these instances, the data reduction convention described above is conservative, consistent with past reporting of PFC data by 3M, and may result in overestimation of actual concentrations. The 3M Environmental Laboratory followed analytical methods described by Malinsky et al. (2011) for the quantification of PFC results for fish tissue samples. Details concerning these methodologies are documented in the complete analytical report that is included as an appendix to this report (see Appendix C). In short, all samples were analyzed using a high performance liquid chromatography (HPLC) system interfaced with a mass spectrometer equipped with an electro-spray interface and operated in negative-ion MS/MS mode. Internal standards and surrogate recovery standards were used to aid in achieving the study data quality objectives. Additional QA/QC measures included the collection of equipment rinseate blanks from tissue processing equipment and the shipment of process control fish samples from the 3M Environmental Laboratory to the field prior to fish collection to be fileted and homogenized for evaluation of fileting, homogenization and the sample shipment processes. This step was intended to confirm adequate decontamination of fileting and J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 2-5 homogenization equipment between preparation of individual study samples and the absence of other contamination vectors associated with the preparation and shipment of the filets and subsequent tissue homogenates. Finally, aliquots of the Standard Reference Material (SRM) 1947 fish tissue homogenate (Lake Michigan lake trout, Salvelinus namaycush) were obtained from the National Institute of Standards and Technology (NIST) by the TestAmerica Burlington laboratory and repackaged into two aliquots with one aliquot sent to AXYS for analysis and the other aliquot sent to the 3M Environmental Laboratory for analysis. Study fish filet samples were assigned unique sample IDs of the form MSFxxx for largemouth bass (where MSF = Micropterus salmoides filet) or IPFxxx (where IPF = Ictalurus punctatus filet) in order to provide blind samples to the laboratories. Sample numbers (xxx) were assigned sequentially in order of sample preparation. A cross reference was maintained by WESTON with full sample IDs denoting location and sample collection date information for each of the specimens. 2.2 SURFACE WATER SAMPLING In addition to collecting fish samples, surface water sampling was performed at each of the 13 fish sampling reaches and eight transects across the Wheeler Reservoir and two fish sampling reaches and one transect across the Wilson Reservoir (Figure 2-1). Three surface water samples were collected at each of the fish sampling reaches between November 16 and 20, 2012 in conjunction with fish sampling activities. After completion of fish collection, nine previously agreed upon transects were established approximately perpendicular to the river flow for sampling surface water (Figure 2-1). The surface water transects extended across the width of the Tennessee River but terminated approximately 75 feet from either shoreline. Ten surface water samples were collected from evenly spaced locations along each of the nine transects between December 3 and 5, 2012, as depicted in Figure 2-1. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 2-6 These nine transects are described as follows: T-1 Transect 1 - Below Triana, AL and coincides with the fish sampling Reach 01 located below Triana, AL at RM320. T-2 Transect 2 - Coincides Reach 02 and Reach 03 at approximately RM307.5. This transect spanned the main flow channel as well as the backwater area to the north of the main channel. T-3 Transect 3 - Immediately downstream from the mouth of Betty Rye Creek and Dry Branch and coincides with Reach 04 at approximately RM303. T-4 Transect 4 - Immediately downstream from the mouth of Bakers Creek and coincides with Reach 05 at approximately RM301 and Reach 06 at approximately RM301.2. T-5 Transect 5 - Immediately downstream from the mouth o f Round Island Creek and coincides with the fish sampling Reach 07 and Reach 08 at approximately RM298. T-6 Transect 6 - Immediately downstream from the mouth of Fox Creek and coincides with the Reach 09 at approximately RM296. T-7 Transect 7 - Immediately downstream from the mouth of Mallard Creek and coincides with Reach 11 at approximately RM293.5. T-8 Transect 8 - Below Spring Creek and the Elk River and coincides with Reach 13 at approximately RM281. T-9 Transect 9 - Below Shoal Creek and coincides with Reach 15 at approximately RM264. This is the only surface water sampling transect within Wilson Reservoir. Surface water samples were collected using a peristaltic pump. Sampling depths were determined on the basis of the total water depth at each sampling location. For surface water sampling locations less than 10 feet deep, samples were collected at the 0.6 depth (six-tenths of the total depth of water). For surface water sampling locations greater than or equal to 10 feet deep, samples were collected from the 0.2 and 0.8 depths (two-tenths and eight-tenths of the total depth of water) and composited in equal volumes in the sample containers. Transect sampling location coordinates were predetermined, loaded into global positioning system (GPS) units and used to navigate to the pre-established sampling points. Minor point adjustments were made along the pre-established locations on Transect T2 (nodes 05, 07, 08 and 09) as the locations were either on a sandbar on in an area where water was very J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 2-7 shallow and inaccessible. Additionally, one location (node 10) on Transect T3 was adjusted in the field, as it fell along the northern shoreline of the river. In all instances, surface water samples were adjusted to the closest viable sampling area. Field-determined surface water sampling locations, including the relocated samples and the 45 surface water sampling locations from the fish reaches, were recorded using a GPS unit and are shown on Figure 2-1. Field duplicate surface water samples were collected at a 10% duplicate to primary sample ratio and shipped to the 3M Environmental Laboratory in St. Paul, MN, for analysis. At these same sample locations, as well as one additional location, an additional duplicate surface water sample was collected and forwarded to the AXYS laboratory for analysis. Surface water primary sample results from 3M Environmental Laboratory analyses are tabulated and used in statistical analyses in this report. Analytical results from duplicate samples analyzed by the 3M Environmental Laboratory and the AXYS laboratory were used for verification of analytical accuracy and performance. A detailed interlaboratory comparison of duplicate sample results and a letter from ADEM to 3M acknowledging the good agreement between laboratories and reinforcing the validity of the results of this study are included in Appendix A. On the basis of these results and other QA/QC samples, the study analytical results are valid and meet the data quality objectives. Primary sample results reported by the 3M Environmental Laboratory were based on the analysis of two aliquots from the primary sample volume. The results were reduced to a single value by calculating the average concentration (arithmetic mean) for sets comprised of numeric values. In instances with mixed numeric values and non-numeric values (non-detects), the numeric values were carried through to represent the media concentrations. In these instances, the data reduction convention described above is conservative, consistent with past reporting of PFC data by 3M, and may result in overestimation of actual concentrations. The 3M Environmental Laboratory followed analytical methods described by W olf et al. (2011) for the quantification of PFC results for water samples. Details concerning these J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 2-8 methodologies are documented in the complete analytical reports that are included as an appendix to this report (see Appendix C). All samples were analyzed using an HPLC system interfaced with a mass spectrometer equipped with an electro-spray interface and operated in negative-ion MS/MS mode. Internal standards and surrogate recovery standards were used to aid in achieving the study data quality objectives. In addition to collecting surface water samples for laboratory analysis of PFC concentrations, routine water quality data, including the parameters of temperature, conductivity, dissolved oxygen, pH and turbidity were collected at the time of sampling at each sampling node. 2.3 STATISTICAL ANALYSIS In data analyses throughout this report, only primary sample results are used. Where duplicate sample results exist, they are used in the interlaboratory comparison of PFC results (see Appendix A) and the interlaboratory and intralaboratory comparison of PFOS results (see Subsection 3.3) to evaluate data quality and are not used in calculations, data summaries, or statistical testing. This report contains a spatial evaluation of the 2012 data and temporal evaluation of the 2012 data and previous analytical data using bar graphs generated in Microsoft Excel and box plots generated in the NCSS8 statistical software (NCSS, 2012). Statistical analyses included the nonparametric Mann-Kendall test for trend performed using the ProUCL Version 4.1.01 statistical software (EPA, 2012) to examine changes over time and the nonparametric Mann-Whitney test using the NCSS8 statistical software to identify statistically significant differences in median concentrations between locations at individual sampling events and within locations over time. The box plots display sample data, including the 1st quartile (25th percentile), the median and the 3rd quartile (75th percentile) values within each box. An example box plot with labeling is provided in Figure 2-2. The whisker below the box represents the data point closest to and greater than or equal to the 25th percentile value minus 1.5 times the interquartile range (IQR; 75th percentile minus the 25th percentile), and the whisker above J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 2-9 the box corresponds to the data point closest to but less than or equal to the 75th percentile value plus 1.5 times the IQR. Outliers are shown as data points above or below the whiskers. The box plots are useful for qualitative comparisons of median values and the ranges of different data sets. Sample median values are routinely used throughout this report as a measure of central tendency because they are less subject to distortion by the presence of extreme values than other measures and they correspond with the formal statistical test procedure described below. The Mann-Kendall test for trend was performed with a significance level of a = 0.05 on fish and surface water data sets with four or more sampling events over the period of record to identify the temporal variation. Because the Mann-Kendall test is nonparametric, it does not require any assumptions regarding the underlying distribution of the data. The possible outcomes of a Mann-Kendall test are the identification of a statistically significant decreasing trend, no statistically significant trend or a statistically significant increasing trend at the specified level of significance. The Mann-Whitney test (=Wilcoxon Rank Sum; WRS) was used to determine whether median concentrations were statistically significantly different (at a significance level of a = 0.05) between sampling locations (spatial evaluation) and at individual sampling locations over time (temporal evaluation). The nonparametric Mann-Whitney test does not require any assumptions regarding the underlying data distributions or equality of variances. A parametric equivalent, the t-test, is a robust test for identifying statistically significant differences in arithmetic mean (average) concentrations but requires that both data sets under comparison are drawn from normally distributed populations and that the variances are equal. The majority of the data sets in this study being evaluated are not sufficiently large (i.e., 8 to 10 samples) to allow for distribution goodness-of-fit testing. Because it is not possible to determine whether the requirements of the t-test are met, the Mann-Whitney test was used exclusively to evaluate differences in median concentrations between locations and over time. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 2-10 Shoal Creek Joe Wheeler Dam Elk River Big Nance Creek Spring Creek Mallard Creek Fox Creek Round Island Creek Swan Creek Dry Branch File: Y:\3MDec\MXD\Fish_Reaches_Transects_oct2012_extend.mxd, 6/14/2013 2:56:12 PM, ricksc Facility Boundary | _ _ | Fish Sampling Reach Surface Water Transect Location Fish Reach Surface Water Sampling Locations Transect Locations Transect 1 FISH SAM PLING LOCATIONS: 1. Tennessee River RM320 2. Point Mallard RM307.5 3. Tennessee River RM307.5 4. Mouth o f Betty Rye Creek and Dry Branch RM303 5. Bakers Creek Mouth RM301 6. Swan Creek Mouth RM301.2 7. Tennessee River RM298 8. Round Island Creek Mouth RM298 9. Fox Creek Mouth RM296 10. Browns Ferry RM294 11. Mallard Creek Mouth RM293.5 12. Elk River Mouth RM285 13. Tennessee River RM281 14. Big Nance Creek Mouth RM273 15. Shoal Creek Mouth RM264 Note: Locations are approximate. Imagery Source: ESRI Bing Mapping Service 2011 # 0 1.25 2.5 5 Miles Fall 2012 Fish Sampling Reaches and Surface Water Sampling Locations Tennessee River  m -I mm - Figure 2-2: Example Box Plot Outlier4 33G 22UD Whisker 2 < (Q3) +1.5*IQR 3rd Quartile (Q3) (75thpercentile value) 11 - Median (50thpercentile value) 1st Quartile (Q1) (25thpercentile value) Whisker 3 > (Q1) - 1.5*IQR Notes: 'The interquartile range (IQR) is a measure of the central data distribution; it is calculated by subtracting the concentration at the 25th percentile from the concentration at the 75th percentile [IQR = Q3-Q1] 2Upper whiskers defined as the data point closest to and less than or equal to the 75th percentile plus 1.5 times the IQR 3Lower whiskers defined as the data point closest to and greater than or equal to the 25th percentile minus 1.5 times the IQR 4An outlier is a data point above the upper whisker or below than the lower whisker 3. RESULTS 3.1 FISH A total of 150 fish, including 75 largemouth bass and 75 channel catfish, within the respective ADEM size slot guidance parameters, were collected in the 15 sampling reaches between October 31, 2012 and November 29, 2012. Ten fish, consisting of five largemouth bass and five channel catfish, were collected in each of the 15 sampling reaches. A summary of the overall elapsed time of individual electrofishing runs and an estimate of the number of bass captured and the catch per unit effort (CPUE) is provided in Table 3-1. The collection rate varied by reach and ranged from 0.4 to 6.7 bass per hour, with an average of 3.8 bass per hour. Areas with better bass habitat (deeper water with steep and rocky ledges, e.g., Wilson Reservoir) required fewer electrofishing hours per bass than those areas with shallow and consistent depths (e.g., RM307.5 backwater and Swan Creek). Channel catfish were ubiquitously distributed throughout the river but less abundant than blue catfish (Ictalurus furcatus) in the two Wilson Reservoir fish sampling reaches. Fish lengths, weights and skin-off filet tissue PFOS and other PFC concentrations in nanograms per gram (ng/g; parts per billion [ppb]) on a wet weight basis are tabulated by sampling reach in Table 3-2 for largemouth bass and Table 3-3 for channel catfish. PFOS analytical results for the largemouth bass and channel catfish filet tissue samples are shown in Figure 3-1. The fish filet tissue median, mean and geometric mean concentrations are shown in Figure 3-2. The fish tissue and surface water analytical data packages for analyses performed by the 3M Environmental Laboratory are provided in Appendix C, and the analytical data packages for analyses of fish tissue and surface water split samples performed by the AXYS laboratory are provided in Appendix D. 3.1.1 Largemouth Bass Summary statistics for the largemouth bass filet tissue PFOS concentrations from the primary (right hand filet) samples are provided in Table 3-4. Median largemouth bass J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report'Draf Final Fall 2012 Fish and Surface Water Report.doc 3-1 filet tissue PFOS concentrations ranged from 25.7 ng/g in Reach 03 (backwater area at RM307.5) to 435 ng/g in Reach 05 (Bakers Creek mouth at RM301). Figures 3-2 and 3-3 depict the median PFOS fish tissue concentrations from the fall 2012 fish sampling event spatially by river mile and fish sampling reach. 3.1.2 Channel Catfish Summary statistics for the catfish filet tissue PFOS concentrations obtained by the 3M Environmental Laboratory analysis of primary (right hand filet) samples are provided in Table 3-5. Median catfish filet tissue PFOS concentrations ranged from 1.50 ng/g in Reach 01 (RM320) to 283 ng/g in Reach 05 (Bakers Creek mouth at RM301). Figures 3 2 and 3-4 depict the median channel catfish tissue PFOS concentrations from the fall 2012 fish sampling event spatially by river mile and fish sampling reach. 3.2 SURFACE WATER SAMPLING RESULTS All planned surface water samples were collected, including the three surface water samples collected at each of the 15 fish sampling reaches as well as the 10 samples along each of the nine surface water transects across the river. Duplicate samples were collected in accordance with the Work Plan, and these were submitted to the respective laboratories for analysis. 3.2.1 Surface Water at Fish Sampling Reaches Surface water samples were collected at the three locations within each of the 15 fish sampling reaches for a total of 45 primary samples. At the time of collection of samples for PFC analysis, data on routine water quality parameters were collected and are tabulated in Appendix E. Surface water PFOS and other PFC data in nanograms per milliliter (ng/mL) (ppb) for the fish sampling reaches are provided in Table 3-6, and PFOS results are shown in Figure 3-5. The PFOS concentrations in surface water samples from the fish sampling J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report'Draft Final Fall 2012 Fish and Surface Water Report.doc 3-2 reaches ranged from 0.00639 ppb in Reach 03 (in the backwater area across from Point Mallard at RM307.5) to 0.385 ppb in Reach 05 (Bakers Creek mouth at RM301). 3.2.2 Surface Water at Transect Locations Surface water samples were collected at 10 locations along each o f the nine surface water transect locations for a total of 90 primary samples. At the time of collection of samples for PFC analysis, data on routine water quality parameters were collected and are tabulated in Appendix E. Surface water PFOS and other PFC data in ng/mL (ppb) for the surface water transect locations are provided in Table 3-7, and PFOS results are shown in Figure 3-6. The PFOS concentrations in surface water samples from the surface water transects ranged from 0.00611 ppb in the middle of Transect 3 (at the confluence of Betty Rye Creek and Dry Branch with the Tennessee River at RM303) to 0.138 ppb at the southernmost point along Transect 4 (closest location to Bakers Creek mouth at RM301). The PFOS results for Transect 1, the farthest upstream transect, are consistent (ranging from 0.00613 to 0.00689 ng/mL) with a median PFOS concentration of 0.00653 ng/mL (ppb). For the area studied, these transect results establish the upstream background levels in surface water. The PFOS results for Transect 9, the farthest downstream transect, are consistent (ranging from 0.0190 to 0.0244 ng/mL) with a median PFOS concentration of 0.0203 ng/mL (ppb). 3.3 QUALITY ASSURANCE AND QUALITY CONTROL Field QA/QC samples, including field duplicate and split samples of the primary fish tissue and surface water, and tissue sample processing equipment blank samples were collected as described in Subsections 2.1 and 2.2. Laboratory QA/QC samples included analysis of surrogate spike samples of isotopically-labeled compounds (both laboratories) and matrix spike samples (3M Environmental Laboratory only). Spike recoveries were used to determine the analytical accuracy. On the basis of laboratory control spike results, the 3M Environmental Laboratory and surface water results were reported with analytical method uncertainties of 22% for fish tissue samples and 17% for surface water J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report'Draft Final Fall 2012 Fish and Surface Water Report.doc 3-3 samples. An additional quality control provision included the analysis of aliquots of the NIST SRM 1947 standard reference material for PFOS by each laboratory. The NIST SRM 1947 Certificate of Analysis reports the PFOS concentration of 5.90 0.39 ng/g. PFOS concentrations reported for analyses o f the SRM 1947 split samples were 5.00 ng/g (RPD of 17%) from the 3M Environmental Laboratory and 5.72 ng/g (RPD of 3%) from the AXYS laboratory. The analytical data packages (Appendices B and C) contain detailed accounts o f laboratory QA/QC results. Comparisons of primary sample and field duplicate sample PFOS concentrations obtained from analysis of fish filet tissue samples by the 3M Environmental Laboratory indicated good correspondence with relative percent difference (RPD) values ranging from 0.6 to 19.2% and an average RPD of 6.2% for the study fish (Table 3-8). Comparisons of fish tissue split sample PFOS concentrations obtained by the AXYS laboratory o f left hand filets with the results from the 3M Environmental Laboratory analyses of the corresponding right hand filets indicated similar agreement with RPDs ranging from 0.5 to 18.5% with an average RPD of 8.7% (Table 3-8). Comparisons of primary and duplicate PFOS results in surface water samples from the 3M Environmental Laboratory also showed good correspondence with RPD values ranging from 1.0 to 14.9% with an average of 6.3% (Table 3-8). Comparisons of surface water split sample PFOS results from the AXYS laboratory to the corresponding 3M Environmental Laboratory primary sample results yielded RPD values ranging from 0.8 to 34.1%, with an average o f 13.9% (Table 3-8). Interlaboratory comparisons of analytical results for other PFCs are provided in Appendix A. The overall comparison of PFOS concentrations measured in all fish and surface water duplicate samples indicated very good agreement within and among laboratories and, with the close agreement between the 3M Environmental Laboratory and AXYS laboratory results and the NIST certified analysis of PFOS in the SRM 1947 tissue, the accuracy of PFOS data developed in the fall 2012 study is considered to be very good. Fish tissue sample processing procedural blanks included the preparation o f process control fish samples and the collection of sample processing equipment rinseate blanks. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 3-4 Comparisons of process control fish tissue left side filet PFOS results with those from earlier analyses of right side filet samples from the same specimens indicated close agreement with the exception of the first two samples prepared (the largemouth bass sample DAL-MSF-LS-1001 and the channel catfish sample DAL-IPF-LS-1002. Subsequent review of field records of sample preparation for these two samples including tissue yields and examination of sample homogenates indicated that the samples were inadvertently mislabeled in the field and are noted on Table 3-2 and Table 3-3. PFOS concentrations in aqueous equipment rinseate blanks were below the limit of quantitation (LOQ) of 0.927 nanograms per liter (ng/L; parts per trillion [ppt]) in three samples and slightly above the LOQ at 1.05 ng/L in the remaining sample. Review of the fish tissue sampling blanks indicates that there were no issues associated with carryover of PFOS between fish samples during tissue processing. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 3-5 Legend Facility Boundary Fish Sampling Reaches Notes: - Average PFOS is the average o f primary and duplicate sample analytical results in ng/g, (ppb) -Only primary sample results are included on this figure Imagery Source: ESRI Bing Mapping Service 2011 N W^ E 0 12 4 I Miles Figure 3-1 Fall 2012 Fish Filet Tissue Results PFOS Concentrations Tennessee River Reach 15 S ta tis tic Bass M edian 8 6 .9 Mean 8 4 .9 Geo Mean 8 3 .8 Catfish 7 .3 3 7 .9 5 7 .7 4 Reach 12 S ta tis tic Bass M edian 6 5 .8 Mean 70.1 Geo Mean 6 8 .8 Joe Wheeler Dam Catfish 10.7 8.71 8.10 R e a c h 11 S ta tis tic Bass M edian 145 Mean 162 Geo Mean 156 Elk River Reach 14 S ta tis tic Bass M edian 7 5 .3 Mean 7 3 .3 Geo Mean 7 1 .4 Catfish 8.01 9 .1 2 8 .6 0 Big Nance Creek Reach 13 S ta tis tic Bass M edian 7 1 .0 Mean 7 7 .0 Geo Mean 7 6 .2 Catfish 10.3 9 .7 7 9 .6 6 Spring Creek Catfish 15.7 15.3 14.5 Reach 10 S ta tis tic Bass M edian 104 Mean 134 Geo Mean 122 Catfish 13.4 12.2 11.8 Reach 08 S ta tis tic Bass M edian 8 0 .3 Mean 102 Geo Mean 9 1 .8 Catfish 4 .8 9 8 .3 8 5.90 Round Island I Creek Swan Creek Reach 06 S ta tis tic Bass M edian 5 0 .6 Mean 6 5 .5 Geo Mean 6 0 .4 Catfish 4 .1 5 3.70 3.59 Facility Boundary Fish Sampling Reaches Notes: -Mean = Arithm etic Mean -Geo Mean = Geometric Mean -Only primary sample results were used for these statistical calculations Reach 09 S ta tis tic Bass M edian 259 Mean 232 Geo Mean 226 Catfish 18.6 2 2 .5 2 0 .0 Reach 07 S ta tis tic Bass M edian 262 Mean 286 Geo Mean 275 Catfish 2 9 .2 4 0 .3 3 3 .3 Reach 05 S ta tis tic Bass M edian 435 Mean 405 Geo Mean 345 Fox Creek Catfish 283 256 199 , B V y .'r jo r { W Ll l Reach 04 S ta tis tic Bass M edian 159 Mean 208 Geo Mean 181 * i , n r <*1 f* K jg V Catfish 7 .8 5 19.0 10.6 Reach 02 S ta tis tic Bass M edian 44.1 Mean 4 3 .2 Geo Mean 4 2 .7 Reach 03 S ta tis tic Bass M edian 2 5 .7 Mean 3 5 .2 Geo Mean 3 1 .4 Catfish 2.01 2 .0 9 1.75 Flint Creek Catfish 2 .6 5 2.41 2 .2 8 R e a c h 01 S ta tis tic Bass M edian 3 2 .7 Mean 3 0 .6 Geo Mean 2 9 .7 Catfish 1.50 2 .0 2 1.55 Imagery Source: ESRI Bing Mapping Service 2011 # 0 12 4 Miles File: Y:\3MDec\MXD\Fish_Reach_samp_areas_Tissue_PFOS_Results_Median.mxd, 6/14/2013 2:03:05 PM, ricksc Fall 2012 Fish Filet Tissue Mean Median and Geometric Mean PFOS Concentrations Tennessee River 450 -Q 400 QQ.. bB 350 W) 300 '+O(-D* 250 acui 200 utOoo 150 100 50 0 Figure 3-3: Largemouth Bass Filet Median PFOS Concentrations - Fall 2012 435 R01 (RM 320) Reach 02 & 03 (RM 307.5) Reach 04 (RM 303) Reach 05 & 06 (RM 301) Reach 07 & 08 (RM 298) Reach 09 (RM 296) Reach 10 (RM 294) Reach 11 (RM 293.5) Reach 12 (RM 285) Reach 13 (RM 281) Reach 14 (RM 273) Reach 15 (RM 264) *e ^ ,,aV * * . * * * N o rth sh o re lin e Fish Reach S outh sh o re lin e Shoal Creek R15L1 0.0199 R15L2 0.0184 R15L3 0.0181 R14L1 0.0187 R14L2 0.0190 R14L3 0.0230 Joe Wheeler Dam Big Nance Creek R13L1 0.0248 R13L2 0.0277 R13L3 0.0238 Spring Creek Elk River R12L1 0.0124 R12L2 0.0138 R12L3 0.0133 R11L1 0.0878 R11L2 0.0910 R11L3 0.0695 R10L1 0.00682 R10L2 0.00699 R10L3 0.00649 R08L1 0.00642] R08L2 0.00657 R08L3 0.00767 I (Round Island I Creek Swan Creek R09L1 Mallard Creek 0.103 R09L2 0.125 R09L3 0.0910 R07L1 0.0842 Fox Creek R07L2 0.102 R07L3 0.0348 R05L1 0.385 R05L2 0.0177 R05L3 0.00984 1 Dry Branch R04L1 0.0144 R04L2 0.0180 R04L3 0.0705 R06L1 0.00684] R06L2 0.00672 R06L3 0.00677 R03L1 0.00703] R03L2 0.00734 R03L3 0.00639 R02L1 0.00932 R02L2 0.00906 R02L3 0.00894 Flint Creek R01L1 0.00691 I R01L2 0.00762 R01L3 0.00669 Legend Fish Reach Surface f t 'ater Sampling Locations Facility Boundary | _ _ | Fish Sampling Reaches Notes: - Average PFOS is the average o f primary and duplicate sample analytical results in parts per billion (ppb) - Only the primary sample results are included on the figure - R01L1 - Reach 01 Location 1 - Significant digits determined by analytical data package (data reported in ng/L, ppt) Imagery Source: ESRI Bing Mapping Service 2011 # 0 12 4 Miles File: Y:\3MDec\MXD\Fish_Reach_samp_areas_PFOS_PPB.mxd, 6/13/2013 2:45:20 PM, ricksc Fall 2012 Surface Water - Fish Reaches PFOS Concentrations Tennessee River T910 T909 T908 T907 T906 T905 T904 T903 T902 0.0190 0.0192 0.0203 0.0214 0.0197 0.0244 0.0207 0.0201 0.0205 0.0203 Joe Wheeler Da Elk River Big Nance Creek T810 T809 T808 T807 T806 T805 T804 T803 T802 0.0123 0.0115 0.0133 0.0141 0.0121 0.0154 0.0166 0.0159 0.0154 0.0152 Spring Creek T710 T709 T708 T707 T706 T705 T704 T703 T702 0.0176 0.0134 0.0165 0.0202 0.0242 0.0312 0.0316 0.0468 0.0466 0.0426 T610 T609 T608 T607 T606 T605 T604 T603 T602 0.00783 0.00766 0.00692 0.00649 0.00972 0.0145 0.0238 0.0566 0.107 0.0864 Round Island Creek Swan Creek T510 T509 T508 T507 T506 T505 T504 T503 T502 0.00857 0.00815 0.00820 0.00810 0.00682 0.00720 0.00789 0.0492 0.0765 0.0728 T410 T409 T408 T407 T406 T405 T404 T403 T402 0.00778 0.00673 0.00704 0.00731 0.00742 0.00821 0.00729 0.00752 0.00869 0.138 Dry Branch T310 T309 T308 T307 T306 T305 T304 T303 T302 0.00732 0.00658 0.00698 0.00657 0.00611 0.00730 0.00768 0.00694 0.00746 0.0190 -lint Creek T210 T209 T208 T207 T206 T205 T204 T203 T202 0.00777 0.00698 0.00644 0.00683 0.00679 0.00696 0.00623 0.00650 0.00993 0.00835 T110 T109 T108 T107 T106 T105 T104 T103 T102 0.00670 0.00645 0.00660 0.00638 0.00643 0.00663 0.00689 0.00680 0.00615 0.00613 File: Y:\3MDec\MXD\Fish_Reach_Transect_PFOS_Results_PPB.mxd, 6/13/2013 11:53:11 AM, ricksc Legend Facility Boundary Surface Water Transect Location A Transect Locations Transect 1 Notes: - Average PFOS is the average o f primary and duplicate sample analytical results in parts per billion (ppb) - Only the primary sample results are included on the figure - Sample node numbers are in numerical order across transects from south (Location 01) to north (Location 10) - T110 = Transect 1 Location 10 - Significant digits determined by analytical data package (data reported in ng/L, ppt) Imagery Source: ESRI Bing Mapping Service 2011 # 0 12 4 I Miles Figure 3-6 Fall 2012 Surface Water - Transects PFOS Concentrations Tennessee River Table 3-1 Fish Sampling Summary and Catch Per Unit Effort Date 10/31/2012 10/31/2012 11/1/2012 11/1/2012 11/1/2012 11/2/2012 11/2/2012 11/5/2012 11/6/2012 11/7/2012 11/8/2012 11/8/2012 11/8/2012 11/9/2012 11/13/2012 11/13/2012 11/14/2012 11/14/2012 11/14/2012 11/14/2012 11/15/2012 11/15/2012 11/16/2012 11/20/2012 11/20/2012 11/20/2012 11/20/2012 11/26/2012 11/28/2012 Run 1 2 1 2 3 1 2 1 1 1 1 2 3 1 1 2 1 2 3 4 1 2 1 1 2 3 4 1 2 Reach R09 R09 R11 R10 R10 R13 R12 R14 R15 R01 R01 R01 R01 R02 R03 R03 R04 R04 R04 R06 R04 R06 R06 R11 R09 R08 R07 R05 R06 Hours 0.5 0.8 0.8 0.4 0.8 0.8 1.0 0.8 0.8 0.8 1.0 0.5 0.8 2.0 2.1 2.0 1.0 1.2 1.4 1.5 1.1 3.3 2.3 0.3 0.3 2.5 1.0 1.0 1.5 Timer (hours) 0.15 0.35 0.37 0.2 0.41 0.42 0.47 0.28 0.36 -- -- 0.45 0.38 -- -- -- -- -- -- -- 0.63 -- -- -- -- -- -- -- -- Timer (seconds) 531 1250 1339 715 1488 1517 1683 1015 1285 -- -- 1621 1362 -- -- -- -- -- -- -- 2281 -- -- -- -- -- -- -- -- CPUE (bass/hour) 4.00 1.3 4.0 7.1 2.7 6.0 5.0 6.7 6.0 0.0 1.0 6.0 1.2 2.5 1.0 1.5 0.0 0.9 0.7 0.0 2.8 0.6 0.4 6.0 6.0 2.0 5.0 5.0 1.3 Summary Reach No. R01 R02 R03 R04 R05 R06 R07 R08 R09 R10 R11 R12 R13 R14 R15 Reach Below Triana, AL (RM320) Point Mallard (RM307.5) Point Mallard Backwater (RM307.5) Betty Rye Creek & Dry Branch (RM303) Bakers Creek (RM301) Swan Creek (RM301.2) Tennessee RM298 Round Island Creek (RM298) Fox Creek (RM296) Browns Ferry (RM294) Mallard Creek (RM293.5) Elk River (RM285) Tennessee RM281 Big Nance Creek (RM273) Shoal Creek RM264 Overall Runs 4 1 2 4 1 4 1 1 3 2 2 1 1 1 1 29 Total Hours 3.08 2.00 4.07 4.68 1.00 8.50 1.00 2.50 1.58 1.17 1.08 1.00 0.83 0.75 0.83 34.08 Total Bass (count) 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 75 Average CPUE (bass/hour) 2.1 2.5 1.2 1.1 5.0 0.4 5.0 2.0 3.8 4.9 5.0 5.0 6.0 6.7 6.0 3.8 Notes: CPUE = Catch per unit effort Sampling times are estimated for sampling time on the river and do not include field prep time, sample prep time and/or related activities. -- = Data not recorded Sample ID MSF071 MSF074 MSF075 MSF080 MSF081 MSF084 MSF085 MSF086 MSF087 MSF089 MSF109 MSF111 MSF115 MSF119 MSF124 MSF090 MSF091 MSF092 MSF099 MSF102 MSF169 MSF170 MSF172 MSF173 MSF174 MSF094 MSF096 MSF125 MSF167 MSF168 MSF127 MSF128 MSF129 MSF130 MSF131 MSF141 MSF142 MSF144 MSF145 MSF146 MSF001 MSF004 MSF006 MSF139 MSF140 MSF030 MSF033 MSF035 MSF037 MSF040 MSF042 MSF044 MSF045 MSF157 MSF158 Table 3-2 Largemouth Bass Fish Morphometrics and PFC Concentrations Date 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/15/2012 11/15/2012 11/16/2012 11/29/2012 11/29/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/21/2012 11/21/2012 Analytical Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental Sampling Reach No. R01 R01 R01 R01 R01 R02 R02 R02 R02 R02 R03 R03 R03 R03 R03 R04 R04 R04 R04 R04 R05 R05 R05 R05 R05 R06 R06 R06 R06 R06 R07 R07 R07 R07 R07 R08 R08 R08 R08 R08 R09 R09 R09 R09 R09 R10 R10 R10 R10 R10 R11 R11 R11 R11 R11 Sampling Reach RM 320 RM 320 RM 320 RM 320 RM 320 Point Mallard Point Mallard Point Mallard Point Mallard Point Mallard RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) Betty Rye Creek & Dry Branch Betty Rye Creek & Dry Branch Betty Rye Creek & Dry Branch Betty Rye Creek & Dry Branch Betty Rye Creek & Dry Branch Bakers Creek Bakers Creek Bakers Creek Bakers Creek Bakers Creek Swan Creek Swan Creek Swan Creek Swan Creek Swan Creek River Mile 281 River Mile 281 River Mile 281 River Mile 281 River Mile 281 Round Island Creek Round Island Creek Round Island Creek Round Island Creek Round Island Creek Fox Creek Fox Creek Fox Creek Fox Creek Fox Creek Browns Ferry Browns Ferry Browns Ferry Browns Ferry Browns Ferry Mallard Creek Mallard Creek Mallard Creek Mallard Creek Mallard Creek River Mile RM320 RM320 RM320 RM320 RM320 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM303 RM303 RM303 RM303 RM303 RM301 RM301 RM301 RM301 RM301 RM301.2 RM301.2 RM301.2 RM301.2 RM301.2 RM281 RM281 RM281 RM281 RM281 RM298 RM298 RM298 RM298 RM298 RM296 RM296 RM296 RM296 RM296 RM294 RM294 RM294 RM294 RM294 RM293.5 RM293.5 RM293.5 RM293.5 RM293.5 Length (inches) 13 3/8 16 1/4 13 1/8 13 1/8 14 1/8 16 1/2 18 15 5/8 17 1/8 13 5/8 15 1/4 16 1/8 15 7/8 16 1/8 17 1/2 17 1/2 14 1/2 17 3/4 13 1/2 16 1/8 16 7/8 16 3/4 17 3/4 15 3/8 17 1/4 17 3/8 17 3/8 17 3/8 15 3/8 15 7/8 15 14 7/8 13 1/4 13 1/4 13 1/4 15 1/4 14 5/8 15 3/8 15 3/8 17 1/2 17 3/8 14 1/2 12 3/8 17 1/2 16 3/8 13 17 13 13 3/8 13 1/8 15 5/8 14 1/8 15 14 16 1/2 Weight (pounds) 1.3 2.5 1.1 1.0 1.2 2.6 3.0 2.1 2.4 1.1 1.9 2.5 2.2 2.7 3.1 3.4 1.8 2.9 1.4 2.7 2.4 2.5 3.1 2.2 3.1 2.9 3.0 2.5 2.3 2.4 2.1 1.8 1.2 1.3 1.2 2.1 1.7 1.8 2.0 3.2 1.6 1.1 0.7 2.8 2.6 1.1 2.8 1.1 1.3 1.1 1.8 1.6 2.2 1.4 3.0 PFBA <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <1.00 <1.00 <1.00 <1.00 <1.00 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 0.860 [a] <0.250 <1.00 <0.250 <0.250 <1.00 <1.00 <1.00 <1.00 <0.250 [c] <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.100 <0.100 <0.250 <0.250 PFHxA <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.101 0.260 [a] <2.52 <2.52 <2.52 <2.52 <2.52 0.151 [a] 0.174 [a] <0.101 <0.101 <0.101 <0.252 <0.252 <0.252 <0.252 0.294 [a] 0.132 0.144 <2.52 <0.252 <0.252 <2.52 <2.52 <2.52 <2.52 <0.101 0.136 <0.101 0.122 [a] <0.101 <0.101 <0.0503 <0.0503 <0.0503 0.0942 [a] <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.252 0.317 [a] PFHpA <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0.110 [a] <0.101 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.503 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 0.198 [a] <0.101 <0.101 <0.101 <0.101 0.120 [a] 0.118 [a] <0.101 <0.252 <0.252 PFC Concentrations (ng/g; ppb) PFOA <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 <0.480 <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 0.643 <0.480 <0.480 <0.240 <0.240 <0.240 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 <0.480 <0.480 <0.240 <0.240 <0.480 <0.480 <0.480 <0.480 <0.480 1.57 [a] <0.240 <0.240 <0.480 <0.480 PFBS <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 2.21 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 0.444 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <1.10 <1.10 <1.10 <0.252 <0.252 0.590 0.459 0.219 0.582 [b] 0.259 0.131 [b] <0.252 <0.252 <0.503 <0.503 PFHS <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.100 <0.100 <0.100 <0.100 <0.100 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 0.783 0.391 <0.250 <0.500 <0.250 <0.250 <0.100 <0.250 <0.250 0.416 <0.100 0.123 <0.100 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 PFOS 35.8 32.7 27.9 18.6 37.8 44.1 32.6 49.5 49.0 40.8 21.8 25.7 31.2 23.9 73.2 104 159 434 124 218 435 373 96.4 599 521 69.0 46.9 40.8 [d] 50.6 120 408 262 250 333 178 134 45.0 76.9 175 80.3 259 167 173 279 281 65.2 190 207 101.9 104 210 [b] 116 225 115 145 FBSA 0.555 1.72 0.648 0.361 0.802 7.01 2.72 6.87 25.8 14.8 1.03 1.57 4.28 2.26 6.47 28.4 20.4 8.55 6.10 22.4 67.2 [b] 567 45.8 135 95.0 28.1 2.95 6.23 12.1 20.2 182 110 137 119 128 [c] 114 [c] 15.4 [c] 11.4 [c] 15.2 [c] 17.7 [c] 86.2 [b,c] 58.2 [c] 79.5 [c] 72.9 [c] 133 [c] 22.1 [c] 31.5 [c] 41.3 [c] 63.6 [c] 35.6 [c] 52.8 [c] 107 37.9 74.7 67.3 FOSA 0.137 0.215 0.122 0.148 0.135 [a] 1.44 0.580 0.127 [a] 0.379 1.22 [b] <0.101 0.235 [b] <0.101 0.162 0.258 [b] 1.71 2.24 4.69 1.60 1.37 [b] 7.44 59.9 2.92 9.75 5.20 0.672 0.396 0.200 [d] 1.28 4.22 4.19 4.00 2.46 8.18 2.12 1.75 0.441 [b] 0.243 [a] 0.348 0.465 2.37 3.05 2.26 1.73 3.75 0.571 1.12 1.28 0.716 1.26 1.25 3.09 1.99 1.20 2.28 N-MeFOSAA <0.100 <0.100 <0.100 <0.100 <0.100 0.398 0.122 [b] <0.100 0.284 0.288 [b] <0.100 <0.100 <0.100 <0.100 <0.100 0.289 0.478 0.688 [b] 0.648 1.39 3.55 22.5 0.545 4.67 1.62 0.446 0.171 <0.100 [d] <0.500 1.21 2.22 1.18 1.65 5.07 1.69 1.06 <0.250 <0.250 1.16 0.336 1.45 1.37 0.962 1.43 1.85 <0.500 1.67 0.596 <0.500 0.798 2.61 [b] 1.06 0.768 [b] 0.786 0.974 N-EtFOSAA <0.250 <0.250 <0.250 <0.250 <0.250 0.530 [b] <0.250 <0.250 0.318 0.322 [a] <0.500 <0.500 <0.500 <0.500 <0.500 0.350 [a] 0.464 0.704 0.478 [b] 1.27 3.01 19.0 0.639 3.46 1.43 <0.250 <0.250 <0.500 [d] <0.500 1.56 1.73 1.08 1.19 2.42 1.43 0.703 <0.250 <0.250 0.896 <0.250 1.19 1.17 0.943 1.45 1.76 0.388 [b] 0.845 0.602 0.425 0.652 1.52 0.791 0.777 0.835 0.847 Table 3-2 Largemouth Bass Fish Morphometrics and PFC Concentrations Sample ID MSF020 MSF021 MSF023 MSF025 MSF027 MSF010 MSF011 MSF013 MSF015 MSF017 MSF046 MSF048 MSF052 MSF056 MSF058 MSF060 MSF061 MSF062 MSF065 MSF067 PRE MID POST DAL-MSF-LS-10001 DAL-MSF-LS-10003 DAL-MSF-LS-10006 Date 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 ___ ___ --11/3/2012 11/3/2012 11/29/2012 Analytical Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental Sampling Reach No. R12 R12 R12 R12 R12 R13 R13 R13 R13 R13 R14 R14 R14 R14 R14 R15 R15 R15 R15 R15 Control Control Control Control Control Control Sampling Reach Elk River Elk River Elk River Elk River Elk River River Mile 281 River Mile 281 River Mile 281 River Mile 281 River Mile 281 Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek na na na na na na River Mile RM285 RM285 RM285 RM285 RM285 RM281 RM281 RM281 RM281 RM281 RM273 RM273 RM273 RM273 RM273 RM264 RM264 RM264 RM264 RM264 na na na na na na Length (inches) 13 5/8 15 3/8 14 5/8 15 13 3/8 13 5/8 16 7/8 13 5/8 13 13 1/4 13 3/8 13 3/8 14 15 1/8 17 1/8 16 3/8 14 17 3/8 17 1/8 15 7/8 ___ ___ --17 1/4 16 3/8 16 Weight (pounds) 1.3 2.2 1.8 1.8 1.5 1.4 3.0 1.4 1.3 1.2 1.4 1.5 1.5 2.1 2.7 2.7 1.7 3.1 2.8 2.3 ------- ------- PFC Concentrations (ng/g; ppb) PFBA <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.250 1.42 1.42 1.42 <0.250 <0.250 <0.250 PFHxA <0.0503 <0.0503 <0.101 <0.101 <0.101 <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.0503 <0.101 0.126 [a] 0.170 [a] <0.252 <0.252 <0.252 PFHpA PFOA <0.252 <0.252 <0.101 <0.101 <0.101 <0.252 <0.252 <0.252 <0.252 <0.252 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0.176 [a] <0.503 <0.503 <0.252 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 0.576 [a,c] <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.960 <0.240 <0.240 <0.252 <0.252 <0.252 <0.240 <0.240 <0.240 PFBS <1.10 <1.10 0.184 [b] 0.294 [a] 0.176 [b] <1.10 NR <1.10 <1.10 <1.10 <0.252 0.269 [a] <0.252 <0.252 <0.252 <0.252 0.289 <0.252 <0.252 <0.252 <0.101 <0.252 <0.252 <0.252 <0.252 <0.252 PFHS <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.0960 <0.250 <0.250 <0.500 <0.500 <0.500 PFOS 65.8 98.2 54.9 66.4 65.3 71.0 98.5 68.9 67.1 79.6 54.1 88.9 93.5 [b] 54.8 75.3 64.7 75.5 103 86.9 94.6 0.731 0.523 [a] <0.184 [a] 3.95 3.67 4.31 FBSA 56.7 [c] 23.3 [c] 33.0 [c] 47.9 [b,c] 48.1 [c] 68.6 [c] 81.7 [c] 84.1 [c] 56.2 [c] 51.3 [c] 56.5 129 71.1 19.3 69.7 74.2 85.9 92.6 46.8 38.6 <0.250 [d] <0.250 <0.250 [c] <0.250 <0.250 <0.250 FOSA 1.11 0.699 0.800 0.887 0.988 1.42 2.03 1.37 1.24 1.09 1.11 2.40 2.11 0.530 1.59 2.48 2.21 2.42 1.19 1.98 <0.253 <0.253 <0.253 <0.253 <0.253 <0.253 N-MeFOSAA <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 0.546 [a] 0.684 [a] <0.500 0.435 1.00 0.448 [b] <0.250 0.404 0.365 [b] 0.393 0.419 0.331 0.313 <0.500 <0.100 <0.250 <0.500 <0.500 <0.500 N-EtFOSAA <0.500 0.520 [a] 0.326 0.282 0.344 [b] <0.500 <0.500 <0.500 <0.500 <0.500 0.315 0.795 0.458 0.226 [b] 0.384 0.447 0.544 0.457 0.284 0.493 <0.500 <0.250 <0.250 <0.500 <0.500 <0.500 Notes: PFC concentrations are the average of the primary and duplicate analytical results expressed in ng/g (ppb) on a wet weight basis. na - not applicable -- not supplied NR - Sample had LMS prepared and recovery was outside of 100 + 50% and result not reported. * - quantified from a linear-PFOS (L-PFOS) calibration for total-PFOS (sum of linear and branched isomer peaks) in samples, LMSs and LCSs. [a] Value reported for single replicate results, duplicate result was <LOQ. [b] RPD > 20% [c] Sample had LMS prepared and LMS recovery result was outside of 100 + 30%, but within 100 + 50% and was reported with an expanded uncertainty of + 50%. [d] Value reported for single result, duplicate sample analysis had peak shift out of retention time window and no result determined. PRE - TN_11-0194-1_1 Sample 1_Pre-Study_Homogenization_Process control MID -TN_11-0194-1_1Sample 2_Mid-Study_Homogenization_Process control POST - TN_11-0194-1_1 Sample 3_Post-Study_Homogenization DAL-MSF-LS-10001 = Pre-Sample Filet; Lab Tag No. TN12-0364-1/13 Bass (250904) DAL-MSF-LS-10003 = Lab Tag No. TN12-0364-3/13-Bass (250906) DAL-MSF-LS-10006 = Post-Sample Filet; Lab Tag No. TN12-0364-2/13 Bass (250905) Post-analysis inspection of samples DAL-MSF-LS-10001 and DAL-IPF-LS-10002 indicated the samples had been switched. Table reflects correction. PFOS = Perfluorooctane sulfonate PFBA = Perfluorobutanoic acid PFHxA = Perfluorohexanoic acid PFHpA = Perfluoroheptanoic acid PFOA = Perfluorooctanoic acid PFBS = Perfluorobutane sulfonate PFHS = Perfluorohexane sulfonate FBSA = Perfluorobutanesulfonamide FOSA =Perfluorooctanesulfonamide N-MeFOSAA = 2-(N-ethyl-PFOSA) acetic acid N-EtFOSAA = 2-(N-methyl-PFOSA) acetic acid Sample ID IPF070 IPF076 IPF077 IPF078 IPF079 IPF082 IPF083 IPF112 IPF113 IPF116 IPF117 IPF118 IPF120 IPF122 IPF123 IPF100 IPF101 IPF106 IPF107 IPF108 IPF161 IPF163 IPF164 IPF165 IPF166 IPF095 IPF097 IPF098 IPF104 IPF105 IPF147 IPF148 IPF149 IPF151 IPF152 IPF133 IPF134 IPF135 IPF137 IPF138 IPF012 IPF014 IPF026 IPF029 IPF031 IPF007 IPF009 IPF034 IPF036 IPF153 IPF002 IPF003 IPF039 IPF041 IPF155 Table 3-3 Channel Catfish Fish Morphometrics and PFC Concentrations Date 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/21/2012 Analytical Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental Sampling Reach No. R01 R01 R01 R01 R01 R02 R02 R02 R02 R02 R03 R03 R03 R03 R03 R04 R04 R04 R04 R04 R05 R05 R05 R05 R05 R06 R06 R06 R06 R06 R07 R07 R07 R07 R07 R08 R08 R08 R08 R08 R09 R09 R09 R09 R09 R10 R10 R10 R10 R10 R11 R11 R11 R11 R11 Sampling Reach RM 320 RM 320 RM 320 RM 320 RM 320 Point Mallard Point Mallard Point Mallard Point Mallard Point Mallard RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) Betty Rye Creek & Dry Branch Betty Rye Creek & Dry Branch Betty Rye Creek & Dry Branch Betty Rye Creek & Dry Branch Betty Rye Creek & Dry Branch Bakers Creek Bakers Creek Bakers Creek Bakers Creek Bakers Creek Swan Creek Swan Creek Swan Creek Swan Creek Swan Creek River Mile 298 River Mile 298 River Mile 298 River Mile 298 River Mile 298 Round Island Creek Round Island Creek Round Island Creek Round Island Creek Round Island Creek Fox Creek Fox Creek Fox Creek Fox Creek Fox Creek Browns Ferry Browns Ferry Browns Ferry Browns Ferry Browns Ferry Mallard Creek Mallard Creek Mallard Creek Mallard Creek Mallard Creek River Mile RM320 RM320 RM320 RM320 RM320 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM303 RM303 RM303 RM303 RM303 RM301 RM301 RM301 RM301 RM301 RM301.2 RM301.2 RM301.2 RM301.2 RM301.2 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM296 RM296 RM296 RM296 RM296 RM294 RM294 RM294 RM294 RM294 RM293.5 RM293.5 RM293.5 RM293.5 RM293.5 Length (inches) 18 1/4 17 18 7/8 23 7/8 19 3/4 22 1/8 19 1/2 19 3/8 18 1/4 20 20 7/8 19 3/8 20 7/8 21 3/4 15 7/8 16 5/8 21 3/8 19 1/2 15 1/2 15 1/2 18 1/4 19 1/4 21 1/8 18 5/8 22 5/8 20 5/8 21 1/2 18 5/8 18 1/8 14 7/8 19 3/8 22 1/2 16 1/8 18 5/8 20 3/8 23 3/4 16 3/4 18 1/8 15 3/4 15 5/8 17 3/8 16 1/8 18 1/2 18 1/4 18 1/4 18 7/8 20 5/8 14 17 1/2 19 1/2 20 1/2 18 7/8 14 3/4 16 17 3/8 Weight (pounds) 1.5 1.6 2.5 4.3 2.6 3.6 2.1 2.6 2.0 2.4 2.8 1.7 2.0 3.6 1.2 1.8 2.8 2.5 1.0 0.9 2.0 2.8 3.6 2.3 3.1 2.7 3.4 1.9 1.8 0.9 2.3 3.2 1.3 1.7 2.6 3.9 1.3 1.6 1.0 1.2 1.8 1.2 2.0 2.2 2.1 2.0 3.4 0.8 1.9 2.5 2.2 1.3 0.9 1.1 1.7 PFBA <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <1.00 <1.00 <1.00 <1.00 <1.00 <1.00 <1.00 <1.00 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 2.34 [a] <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 PFHxA <0.0503 0.139 [a] <0.0503 <0.0503 <0.0503 <0.0503 0.137 [a] <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <0.101 <0.101 <0.101 <0.101 <0.101 <0.252 <0.252 <0.252 <0.252 <0.252 0.0965 [a] 0.137 [a] <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.252 <0.252 0.417 [a] <0.101 <0.101 <0.101 <0.101 <0.0503 <0.0503 1.78 [a] <0.101 <0.101 <0.0503 <0.0503 <0.101 <0.101 <0.252 <0.0503 <0.0503 <0.101 <0.101 <0.252 PFC Concentrations (ng/g; ppb) PFHpA <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.503 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.503 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 1.79 [a] <0.101 <0.101 <0.252 <0.252 <0.101 <0.101 <0.252 <0.252 <0.252 <0.101 <0.101 <0.252 PFOA <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 <0.480 <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 <0.480 3.13 [a] <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 PFBS <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.503 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.252 <1.10 <1.10 0.887 [b] 0.460 1.31 <1.10 <1.10 1.862 0.371 [b] <0.503 <1.10 3.82 0.305 [b] 0.405 [b] <0.503 PFHS <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 1.54 [a] <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 PFOS 1.81 0.768 1.50 5.17 0.836 2.92 3.41 [b] 1.78 1.30 2.65 0.897 2.01 0.961 4.45 2.11 4.69 50.9 2.42 7.85 29.1 85.6 83.4 283 542 286 4.37 4.37 2.22 4.15 3.41 26.9 69.6 64.2 11.7 29.2 22.6 3.98 [b] 4.89 [b] 1.90 8.55 12.1 14.3 45.5 [b] 18.6 21.9 8.86 13.4 8.12 14.4 16.3 8.49 18.3 15.7 22.2 11.9 FBSA <0.250 <0.250 <0.250 3.58 <0.250 0.295 0.512 [b] <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 0.575 3.37 0.609 1.39 1.29 69.2 44.8 37.5 34.1 33.3 1.16 1.90 0.867 1.16 0.633 72.8 [c] 65.9 [c] 45.9 [c] 49.5 106 8.87 [c] 1.80 [c] 3.67 [c] 2.5 [c] 3.97 [c] 96.3 [b,c] 52.4 [c] 19.6 [c] 69.2 [c] 84.2 [c] 4.83 [c] 5.29 [c] 5.38 [c] 7.86 [c] 10.1 20.2 [c] 164 [b,c] 53.7 [c] 35.4 [c] 38.6 FOSA 0.153 [b] <0.101 <0.101 0.277 [b] <0.101 0.196 [a] 0.123 [a] <0.101 <0.101 <0.101 <0.101 <0.101 0.300 <0.101 <0.101 0.656 6.68 0.271 [a] 1.28 1.07 34.1 25.6 63.5 [b] 123 88.1 0.258 0.413 0.299 [a] <0.253 0.255 [a] 7.23 3.64 2.45 1.81 4.92 0.384 <0.253 <0.253 <0.253 <0.253 4.81 2.35 6.38 4.20 3.01 <0.505 0.487 0.884 [b] 0.629 0.816 0.947 [b] 2.23 3.58 2.41 1.83 N-MeFOSAA <0.100 <0.100 <0.100 0.149 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 0.122 [a] 0.236 [b] 0.107 [a] <0.100 0.246 [b] 3.42 1.85 3.51 13.2 7.96 <0.100 0.121 <0.100 0.111 [a] <0.100 0.806 0.351 0.299 <0.500 0.731 <0.250 <0.250 <0.250 <0.250 <0.250 0.829 0.654 [a] 2.4 [a] 1.94 0.729 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 1.07 0.716 1.30 <0.500 N-EtFOSAA <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.250 <0.250 <0.250 <0.250 <0.250 2.66 1.16 1.00 4.30 3.32 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.500 <0.500 <0.250 <0.250 <0.250 <0.250 <0.250 0.511 <0.500 4.73 [a] 0.844 0.281 <0.500 <0.500 <0.250 <0.250 <0.500 <0.500 0.714 0.373 0.381 <0.500 Table 3-3 Channel Catfish Fish Morphometrics and PFC Concentrations Sample ID IPF018 IPF022 IPF159 IPF160 IPF179 IPF024 IPF175 IPF176 IPF178 IPF180 IPF047 IPF049 IPF051 IPF053 IPF055 IPF057 IPF063 IPF066 IPF068 IPF069 IPF181 IPF182 DAL-IPF-LS-10002 DAL-IPF-LS-10004 DAL-IPF-LS-10005 Date 11/3/2012 11/3/2012 11/29/2012 11/29/2012 11/29/2012 11/3/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 12/31/2012 12/31/2012 11/3/2012 11/3/2012 11/29/2012 Analytical Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental Sampling Reach No. R12 R12 R12 R12 R12 R13 R13 R13 R13 R13 R14 R14 R14 R14 R14 R15 R15 R15 R15 R15 Control Control Control Control Control Sampling Reach Elk River Elk River Elk River Elk River Elk River River Mile 281 River Mile 281 River Mile 281 River Mile 281 River Mile 281 Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek NIST SAMPLE NIST SAMPLE na na na River Mile RM285 RM285 RM285 RM285 RM285 RM281 RM281 RM281 RM281 RM281 RM273 RM273 RM273 RM273 RM273 RM264 RM264 RM264 RM264 RM264 na na na na na Length (inches) 15 3/4 15 19 19 5/8 16 1/4 14 3/8 17 1/4 19 1/4 20 5/8 17 1/4 16 1/2 15 3/4 15 1/4 14 7/8 16 3/4 16 3/8 14 5/8 22 21 1/8 16 3/4 ----- 16 4/8 15 6/8 17 1/2 Weight (pounds) 1.3 0.8 1.9 2.4 1.3 0.9 1.9 2.1 2.9 1.4 1.5 1.0 0.7 0.9 1.6 1.2 0.9 3.7 2.7 0.8 ----- ------- PFBA <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.250 <0.250 <0.250 <0.250 <0.450 <0.250 <0.250 <0.250 PFHxA <0.0503 <0.0503 <0.252 <0.252 <0.252 <0.101 <0.252 <0.252 <0.252 0.277 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.0503 <0.0503 <0.0503 <0.101 <0.450 <0.252 <0.252 <0.252 PFHpA <0.252 <0.252 <0.252 <0.252 <0.252 <0.101 <0.252 <0.252 <0.252 <0.252 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0.138 [a] <0.252 <0.450 <0.252 <0.252 <0.252 PFC Concentrations (ng/g; ppb) PFOA <0.480 <0.480 <0.480 <0.480 <0.240 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.450 <0.240 <0.240 <0.240 PFBS <1.10 <1.10 <0.503 <0.503 0.506 2.668 <0.252 <0.252 0.322 <0.252 0.327 <0.252 0.337 1.54 [c] <0.252 <0.252 0.854 0.353 [b] <0.252 0.739 <0.252 <0.901 <0.252 <0.252 <0.252 PFHS <0.250 <0.250 <0.250 <0.250 <0.500 <0.250 <0.500 <0.500 <0.500 <0.500 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.901 <0.500 <0.500 <0.500 PFOS 11.7 5.10 11.2 4.87 10.7 10.3 8.57 11.8 10.5 7.69 14.3 5.59 8.01 6.68 11.0 7.33 6.67 10.5 [c] 9.60 5.63 5.00 5.72 <0.366 <0.366 <0.366 FBSA 28.6 [c] 36.9 [c] 20.4 8.47 35.7 12.3 [c] 36.2 28.6 51.2 27.8 63.9 20.6 28.0 15.9 31.5 22.8 16.0 37.5 40.8 18.0 <0.250 [c] --- <0.250 <0.250 <0.250 FOSA 1.21 <0.505 0.620 0.490 0.670 1.07 [b] 1.36 1.54 1.23 1.43 2.71 1.29 1.71 1.31 1.37 [b] 1.58 1.43 1.95 1.48 0.771 <0.253 <0.541 <0.253 <0.253 <0.253 N-MeFOSAA <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 0.539 0.293 <0.250 0.307 <0.250 0.333 [b] <0.250 0.300 0.174 0.198 0.142 <0.250 --- <0.500 <0.500 <0.500 N-EtFOSAA <0.500 <0.500 <0.500 <0.500 <0.500 0.26 [a] <0.500 <0.500 <0.500 <0.500 0.116 [a] 0.141 [a] <0.100 <0.100 0.113 [b] 0.102 [a] 0.147 0.344 0.279 <0.250 <0.250 --- <0.500 <0.500 <0.500 Notes: P F C co n ce n tra tio n s a re th e a ve ra g e o f th e prim ary and d u p lic a te a n a lytica l re su lts e xp ressed in ng/g (ppb) on a w e t w e ig h t basis. na - not applicable -- not supplied N R - S a m ple had LM S prepared and recovery w a s outside o f 100 + 50% and result not reported. * - q u a n tifie d fro m a lin e a r-P F O S (L -P F O S ) c a lib ra tio n fo r to ta l-P F O S (s u m o f lin e a r and b ra n c h e d is o m e r p e a k s ) in s a m p le s , L M S s and LC S s. [a] V a lue reported fo r single replicate results, dup lica te result w a s <LOQ. [b] R P D > 20% [c] S a m ple had LM S prepared and LM S recovery result w a s outsid e o f 100 + 30% , but w ith in 100 + 50% and w a s reported w ith an expa nded unce rtain ty o f + 50% . [d] V a lue reported fo r single result, dup lica te sam ple a na lysis had peak shift out o f retention tim e w in d o w and no result determ ined D A L-IP F -L S -10002 = P re-S a m ple Filet; Lab T ag No. T N 12-0365-1/13 C a t_fish (250919) D A L-IP F -LS -10004 = Lab Tag No. T N 12-0365-2/12 C at_fish (250918) D A L-IP F -L S -10005 = P o st-S am ple Filet; Lab Tag No. T N 12-0385-1/12 C at_fish (250917) Post-analysis inspection of samples DAL-MSF-LS-10001 and DAL-IPF-LS-10002 indicated the samples had been switched. Table reflects correction. PFOS = P erfluorooctane sulfonate P F BA = P erfluo rob utan oic acid P F H xA = Perfluo roh exanoic acid P F H pA = P e rfluo roh epta noic acid P F O A = P erfluorooctanoic acid PFBS = P erfluorobutane sulfonate PFHS = P erfluorohexane sulfonate FB S A = P e rfluorobutanesulfonam ide F O S A =P erflu oro octan esulfona m ide N -M eFO S A A = 2-(N -ethyl-P FO S A ) acetic acid N -E tFO S A A = 2-(N -m ethyl-P F O S A ) acetic acid T a b le 3-4 L a rg e m o u th B a ss F ile t PFOS C o n c e n tra tio n S u m m a ry S ta tis tic s Fall 2012 Reach R e a c h 01 Reach 02 Reach 03 Reach 04 Reach 05 Reach 06 Reach 07 Reach 08 Reach 09 Reach 10 R e a ch 11 Reach 12 Reach 13 R each 14 R each 15 S a m p lin g Reach B e low Triana, AL P oint M allard P oint M allard (B ackw ater) Betty Rye C reek & Dry Branch Bakers Creek Swan Creek R iver M ile 298 R ound Island C reek Fox Creek B row ns Ferry M allard C reek Elk River R iver M ile 281 Big N a nce C reek Shoal Creek R iv e r M ile R M 320 R M 307.5 R M 307.5 R M 303 RM301 RM 301.2 R M 298 R M 298 R M 296 R M 294 R M 293.5 R M 285 RM281 R M 273 R M 264 M in im u m 18.6 32.6 21.8 104 96.4 41 178 45 167 65.2 115 54.9 67.1 54.1 64.7 M a xim u m 37.8 49.5 73.2 434 599 120 408 175 281 207 225 98.2 98.5 93.5 103 Notes: PFOS = Perfluorooctane sulfonate PFOS concentrations are the average of the prim ary and duplicate analytical results expressed in ng/g (ppb) on a wet weight basis. Summary statistics obtained from prim ary data (e.g. field duplicates/ split sample data not included). M ed ia n 32.7 44.1 25.7 159 435 50.6 262 80.3 259 104 145 65.8 71.0 75.3 86.9 Mean 30.6 43.2 35.2 208 405 65.5 286 102 232 134 162 70.1 77.0 73.3 84.9 G e o m e tric Mean 29.7 42.7 31.4 181 345 60.4 275 91.8 226 122 156 68.8 76.2 71.4 83.8 S ta n d a rd D e via tio n 7.66 6.93 21.5 134 193 32.3 87.5 51.7 57.1 61.5 52.2 16.4 12.9 18.5 15.2 Table 3-5 Channel Catfish Filet PFOS Concentration Summary Statistics Fall 2012 Reach R e a ch 01 Reach 02 Reach 03 Reach 04 Reach 05 Reach 06 Reach 07 Reach 08 Reach 09 Reach 10 R e a ch 11 Reach 12 Reach 13 Reach 14 Reach 15 Sampling Reach B e low Triana, A L Point M allard Point M allard (B ackw ate r) Betty Rye C reek & Dry Branch Bakers Creek Sw an Creek R iver M ile 298 R ound Island C reek Fox Creek Brow ns Ferry M allard C reek Elk R iver R iver M ile 281 Big N a nce C reek Shoal Creek River Mile RM320 R M 307.5 R M 307.5 RM303 RM301 RM 301.2 RM298 RM298 RM296 RM294 R M 293.5 RM285 RM281 RM273 RM264 Minimum 0.768 1.30 0.897 2.42 83.4 2.22 11.7 1.90 12.1 8.12 8.49 4.87 7.69 5.59 5.63 Maximum 5.17 3.41 4.45 50.9 542 4.37 69.6 22.6 45.5 16.3 22.2 11.7 11.8 14.3 10.5 Notes: PFOS = Perfluorooctane sulfonate Average PFOS concentrations for fish tissue are the average of the primary and duplicate analytical results expressed in ng/g (ppb) on a wet weight basis. Summary statistics obtained from primary data (e.g. field duplicates/ split sample data not included). Median 1.50 2.65 2.01 7.85 283 4.15 29.2 4.89 18.6 13.4 15.7 10.7 10.3 8.01 7.33 Mean 2.02 2.41 2.09 19.0 256 3.70 40.3 8.38 22.5 12.2 15.3 8.71 9.77 9.12 7.95 Geometric Mean 1.55 2.28 1.75 10.6 199 3.59 33.3 5.90 20.0 11.8 14.5 8.10 9.66 8.60 7.74 Standard Deviation 1.82 0.858 1.44 20.8 189 0.919 25.3 8.30 13.4 3.57 5.36 3.42 1.64 3.54 2.04 Sampling Reach No. R01 R02 R03 R04 R05 R06 R07 R08 R09 R10 R11 R12 Sampling Reach RM 320 Point Mallard RM 307.5 (B a ckw ater) Betty R ye C reek & Dry Branch Bakers Creek Swan Creek RM 298 Round Island Creek Fox Creek Browns Ferry M allard C reek Elk River Location Number L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 Sample ID D AL-SW -R 01L1-0-121116 D AL-SW -R 01L2-0-121116 D AL-SW -R 01L3-0-121116 D AL-SW -R 02L1-0-121116 D AL-SW -R 02L2-0-121116 D AL-SW -R 02L3-0-121116 D AL-SW -R 03L1-0-121116 D AL-SW -R 03L2-0-121116 D AL-SW -R 03L3-0-121116 D AL-SW -R 04L1-0-121116 D AL-SW -R 04L2-0-121116 D AL-SW -R 04L3-0-121116 D AL-SW -R 05L1-0-121119 D AL-SW -R 05L2-0-121119 D AL-SW -R 05L3-0-121119 D AL-SW -R 06L1-0-121120 D AL-SW -R 06L2-0-121120 D AL-SW -R 06L3-0-121120 D AL-SW -R 07L1-0-121119 D AL-SW -R 07L2-0-121119 D AL-SW -R 07L3-0-121119 D AL-SW -R 08L1-0-121119 D AL-SW -R 08L2-0-121119 D AL-SW -R 08L3-0-121119 D AL-SW -R 09L1-0-121119 D AL-SW -R 09L2-0-121119 D AL-SW -R 09L3-0-121119 D AL-SW -R 10L1-0-121119 D AL-SW -R 10L2-0-121119 D AL-SW -R 10L3-0-121119 D AL-SW -R 11L1-0-121119 D AL-SW -R 11L2-0-121119 D AL-SW -R 11L3-0-121119 D AL-SW -R 12L1-0-121117 D AL-SW -R 12L2-0-121117 D AL-SW -R 12L3-0-121117 Date 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 6 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 0 1 1 /2 0 /2 0 1 2 1 1 /2 0 /2 0 1 2 1 1 /2 0 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 9 /2 0 1 2 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 Time 10:45 11:15 11:40 12:50 13:05 13:20 13:50 14:00 14:08 14:45 15:00 15:10 16:25 16:35 16:45 16:15 16:30 16:40 15:10 15:25 15:40 14:05 14:20 14:35 11:40 11:50 12:05 10:35 10:48 11:00 9:35 9:45 10:00 16:20 16:35 16:50 Table 3-6 Surface Water PFC Data - Fish Reach Locations Analytical Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory PFBA 0.00616 0.00650 < 0 .0 0 5 0 0 0.00736 0.00606 0.00613 0.00595 0.00577 0.00500 0.00751 0.00727 0.0117 0.0589 0.00651 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0236 0.0309 0.00941 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00841 0.0295 0.0369 0.0265 0.00716 0.00520 < 0 .0 0 5 0 0 0.0225 0.0242 0.0167 0.00546 0.00689 0.00663 PFHxA 0.00256 0.00283 0.00272 0.00452 0.00556 0.00483 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00285 0.00985 0.0138 0.0124 0.0725 0.00443 0.00347 0.00207 < 0 .0 0 2 0 0 0.00330 0.0344 0.0414 0.0109 0.00240 < 0 .0 0 2 0 0 0.00348 0.0592 0.0619 0.0495 0.00323 0.00210 0.00273 0.0755 0.0702 0.0477 0.00741 0.00756 0.00661 PFHpA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00347 0.00364 0.00470 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00382 0.00457 0.00391 0.05680 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.0169 0.0208 0.00616 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.0221 0.0254 0.0181 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.0195 0.0226 0.0120 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 PFOA 0.00216 0.00304 0.00231 0.0107 0.0104 0.00928 0.00343 0.00370 0.00334 0.0136 0.0150 0.0317 0.246 0.00902 0.00582 0.00297 < 0 .0 0 4 8 0 0.00276 0.0503 0.0626 0.0211 0.00337 0.00256 0.00470 0.0623 0.0799 0.0580 0.00263 0.00253 0.00352 0.0531 0.0619 0.0387 0.00635 0.00651 0.00709 PFC Concentrations (ng/mL ; ppb) PFBS < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0104 0.124 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0464 0.0638 0.00965 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0641 0.0846 0.0594 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0521 0.0511 0.0289 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 PFHS < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00703 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0110 0.0398 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0164 0.0195 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0197 0.0262 0.0196 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0179 0.0189 0.0113 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 PFOS 0.00691 0.00762 0.00669 0.00932 0.00906 0.00894 0.00703 0.00734 0.00639 0.0144 0.0180 0.0705 0.385 0.0177 0.00984 0.00684 0.00672 0.00677 0.0842 0.102 0.0348 0.00642 0.00657 0.00767 0.103 0.125 0.0910 0.00682 0.00699 0.00649 0.0878 0.0910 0.0695 0.0124 0.0138 0.0133 FBSA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00214 0.00207 0.00486 1.130 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.245 0.352 0.0486 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00208 0.185 0.330 0.197 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.129 0.116 0.0741 0.00475 0.00495 0.00512 FOSA N-MeFOSAA N-EtFOSAA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 0.0211 < 0 .0 0 2 0 0 0.0100 < 0 .0 0 5 0 0 0.0180 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 9 9 8 < 0 .0 1 5 0 0 < 0 .0 0 2 0 0 0.00369 0.00565 < 0 .0 0 2 0 0 0.00214 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 0.00615 0.00771 0.00547 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 0.00565 0.00419 0.00389 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 Page 1 of 2 Sampling Reach No. R13 R14 R15 Sampling Reach RM 281 Big N a n c e C re e k Shoal Creek Location Number L1 L2 L3 L1 L2 L3 L1 L2 L3 SampleID D AL-SW -R 13L1-0-121117 D AL-SW -R 13L2-0-121117 D AL-SW -R 13L3-0-121117 D AL-SW -R 14L1-0-121117 D AL-SW -R 14L2-0-121117 D AL-SW -R 14L3-0-121117 D AL-SW -R 15L1-0-121117 D AL-SW -R 15L2-0-121117 D AL-SW -R 15L3-0-121117 Date 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 1 1 /1 7 /2 0 1 2 Notes: PFC concentrations are the average of the primary and duplicate analytical results expressed in in ng/mL (ppb) -- Not Analyzed PFOS = Perfluorooctane sulfonate PFBA = Perfluorobutanoic acid PFHxA = Perfluorohexanoic acid PFHpA = Perfluoroheptanoic acid PFOA = Perfluorooctanoic acid PFBS = Perfluorobutane sulfonate PFHS = Perfluorohexane sulfonate FBSA = Perfluorobutanesulfonamide FOSA =Perfluorooctanesulfonamide N-MeFOSAA = 2-(N-ethyl-PFOSA) acetic acid N-EtFOSAA = 2-(N-methyl-PFOSA) acetic acid Table 3-6 Surface Water PFC Data - Fish Reach Locations Time 15:15 15:35 15:50 11:10 11:30 11:45 12:25 12:55 13:10 Analytical Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory PFBA 0.00795 0.00865 0.00776 0.00682 0.00641 0.00748 0.00792 0.00743 0.00755 PFHxA 0.0102 0.00859 0.0109 0.00482 0.00532 0.00634 0.00424 0.00460 0.00469 PFHpA 0.00336 0.00326 0.00257 < 0 .0 0 2 0 0 0.00218 0.00219 0.00234 0.00223 0.00207 PFOA 0.0144 0.0182 0.0153 0.0111 0.0115 0.0120 0.0115 0.0128 0.0107 PFC Concentrations (ng/mL ; ppb) PFBS 0.0100 0.0120 0.00983 0.00761 0.00769 0.00847 0.0148 0.0132 0.0141 PFHS < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 PFOS 0.0248 0.0277 0.0238 0.0187 0.0190 0.0230 0.0199 0.0184 0.0181 FBSA 0.0204 0.0244 0.0216 0.0147 0.0143 0.0161 0.0348 0.0328 0.0328 FOSA N-MeFOSAA N-EtFOSAA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 Page 2 of 2 Sampling Reach No. T1 T2 T3 T4 Location Number 01 02 03 04 05 06 07 08 09 10 01 02 03 04 05 06 07 08 09 10 01 02 03 04 05 06 07 08 09 10 01 02 03 04 05 06 07 08 09 10 Sample ID D A L-S W -T101-0-121203 D A L-S W -T102-0-121203 D A L-S W -T103-0-121203 D A L-S W -T104-0-121203 D A L-S W -T105-0-121203 D A L-S W -T106-0-121203 D A L-S W -T107-0-121203 D A L-S W -T108-0-121203 D A L-S W -T109-0-121203 D A L-S W -T110-0-121203 D A L-S W -T201-0-121203 D A L-S W -T202-0-121203 D A L-S W -T203-0-121203 D A L-S W -T204-0-121203 D A L-S W -T205-0-121203 D A L-S W -T206-0-121203 D A L-S W -T207-0-121203 D A L-S W -T208-0-121204 D A L-S W -T209-0-121204 D A L-S W -T210-0-121204 D A L-S W -T301-0-121203 D A L-S W -T302-0-121203 D A L-S W -T303-0-121203 D A L-S W -T304-0-121203 D A L-S W -T305-0-121203 D A L-S W -T306-0-121203 D A L-S W -T307-0-121203 D A L-S W -T308-0-121203 D A L-S W -T309-0-121203 D A L-S W -T310-0-121203 D A L-S W -T401-0-121203 D A L-S W -T402-0-121203 D A L-S W -T403-0-121203 D A L-S W -T404-0-121203 D A L-S W -T405-0-121203 D A L-S W -T406-0-121203 D A L-S W -T407-0-121203 D A L-S W -T408-0-121203 D A L-S W -T409-0-121203 D A L-S W -T410-0-121203 Date 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 1 2 /3 /2 0 1 2 Time 8:40 9:00 9:15 9:30 9:45 9:55 10:05 10:10 10:15 10:25 9:30 9:10 8:50 8:30 9:50 10:05 10:15 10:05 11:10 10:45 12:20 12:10 11:50 12:35 12:45 12:55 13:02 13:08 13:10 13:18 15:55 16:15 14:05 14:50 15:08 15:25 15:37 15:22 15:10 14:55 Table 3-7 Surface Water PFC Data - Transect Locations Analytical Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory PFBA < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00519 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00579 0.00672 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00566 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0381 0.00598 0.00783 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00504 0.00567 PFHxA < 0 .0 0 2 0 0 0.00212 < 0 .0 0 2 0 0 0.00218 0.00216 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00200 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00475 0.00516 0.00220 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00211 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00219 0.00455 0.00214 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.0334 0.00286 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00284 PFHpA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00307 0.00480 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00223 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.0196 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 PFOA < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 0.00251 0.00255 0.00256 0.00257 < 0 .0 0 1 9 2 0.00873 0.00275 < 0 .0 0 1 9 2 0.00973 0.0126 < 0 .0 0 1 9 2 0.00242 0.00301 0.00266 0.00208 0.00302 0.00227 0.00390 0.0152 0.00297 0.00278 0.00215 < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 0.00228 0.0745 0.00454 0.00273 0.00225 < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 0.00387 PFC Concentrations (ng/mL ; ppb) PFBS PFHS PFOS FBSA < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00613 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00615 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00680 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00689 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00663 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00643 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00638 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 0.00938 0.00660 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00645 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00670 < 0 .0 0 5 0 0 0.00835 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00993 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00650 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00623 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00696 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00679 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00683 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00644 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00698 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00777 < 0 .0 0 5 0 0 0.0190 < 0 .0 0 2 0 0 0.00232 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00746 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00694 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00768 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00730 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00611 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00657 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00698 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00658 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 0.285 < 0 .0 0 5 0 0 0.00732 0.0174 0.138 < 0 .0 0 2 0 0 0.453 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00869 0.00281 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00752 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00729 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00821 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00742 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00731 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00704 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00673 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00778 < 0 .0 0 2 0 0 FOSA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.0183 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 N-MeFOSAA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00446 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 N-EtFOSAA < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 Page 1 of 3 Sampling Reach No. T5 T6 T7 T8 Location Number 01 02 03 04 05 06 07 08 09 10 01 02 03 04 05 06 07 08 09 10 01 02 03 04 05 06 07 08 09 10 01 02 03 04 05 06 07 08 09 10 Sample ID D A L-S W -T501-0-121204 D A L-S W -T502-0-121204 D A L-S W -T503-0-121204 D A L-S W -T504-0-121204 D A L-S W -T505-0-121204 D A L-S W -T506-0-121204 D A L-S W -T507-0-121204 D A L-S W -T508-0-121204 D A L-S W -T509-0-121204 D A L-S W -T510-0-121204 D A L-S W -T601-0-121204 D A L-S W -T602-0-121204 D A L-S W -T603-0-121204 D A L-S W -T604-0-121204 D A L-S W -T605-0-121204 D A L-S W -T606-0-121204 D A L-S W -T607-0-121204 D A L-S W -T608-0-121204 D A L-S W -T609-0-121204 D A L-S W -T610-0-121204 D A L-S W -T701-0-121204 D A L-S W -T702-0-121204 D A L-S W -T703-0-121204 D A L-S W -T704-0-121204 D A L-S W -T705-0-121204 D A L-S W -T706-0-121204 D A L-S W -T707-0-121204 D A L-S W -T708-0-121204 D A L-S W -T709-0-121204 D A L-S W -T710-0-121204 D A L-S W -T801-0-121205 D A L-S W -T802-0-121205 D A L-S W -T803-0-121205 D A L-S W -T804-0-121205 D A L-S W -T805-0-121205 D A L-S W -T806-0-121205 D A L-S W -T807-0-121205 D A L-S W -T808-0-121205 D A L-S W -T809-0-121205 D A L-S W -T810-0-121205 Date 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /4 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 Time 11:00 10:50 10:20 10:35 11:22 11:30 11:38 11:50 11:57 12:05 13:40 13:32 13:26 13:20 13:10 12:55 12:47 12:40 12:35 12:30 14:35 14:45 15:00 15:10 15:20 15:20 15:10 15:00 14:53 14:45 9:00 9:08 9:20 9:30 9:40 9:52 10:00 10:10 10:23 10:35 Table 3-7 Surface Water PFC Data - Transect Locations Analytical Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory PFBA 0.0237 0.0243 0.0144 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00502 0.00856 0.0432 0.0233 0.0139 0.00809 0.00748 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00572 0.00874 0.0181 0.0157 0.0114 0.00859 0.00971 0.0101 0.00842 0.00696 0.00735 0.00839 0.00689 0.00682 0.00903 0.00776 0.00801 0.00579 0.00626 0.00628 0.00650 0.00562 PFHxA 0.0267 0.0245 0.0137 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00399 0.0694 0.0278 0.0146 0.00790 0.00565 0.00231 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00275 0.0175 0.0158 0.0123 0.00817 0.00949 0.00739 0.00405 0.00390 0.00420 0.00525 0.00458 0.00433 0.00380 0.00446 0.00392 0.00380 0.00295 0.00451 0.00280 0.00279 PFHpA 0.0144 0.0142 0.00919 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.0273 0.0220 0.00962 0.00485 0.00337 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00858 0.00941 0.00823 0.00478 0.00615 0.00583 0.00324 0.00282 0.00232 0.00352 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00281 < 0 .0 0 2 0 0 0.00307 0.00203 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 PFOA 0.0482 0.0457 0.0290 < 0 .0 0 1 9 2 0.00252 0.00259 0.00220 0.00333 0.00327 0.00433 0.0692 0.0686 0.0368 0.0146 0.00937 0.00438 < 0 .0 0 1 9 2 < 0 .0 0 1 9 2 0.00261 0.00312 0.0281 0.0307 0.0298 0.0185 0.0189 0.0145 0.0125 0.00798 0.00686 0.0107 0.00851 0.00943 0.0102 0.00781 0.00808 0.00730 0.00721 0.00749 0.00726 0.00649 PFC Concentrations (ng/mL ; ppb) PFBS PFHS PFOS FBSA 0.105 0.0143 0.0728 0.139 0.115 0.0126 0.0765 0.168 0.0638 0.00835 0.0492 0.0964 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00789 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00720 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00682 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00810 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00820 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00815 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 0.0896 < 0 .0 0 5 0 0 0.00857 0.0242 0.0864 0.00229 0.254 0.124 0.0180 0.107 0.168 0.0570 0.00919 0.0566 0.0753 0.0237 < 0 .0 0 5 0 0 0.0238 0.0321 0.0124 < 0 .0 0 5 0 0 0.0145 0.0158 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00972 0.00222 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00649 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00692 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00766 < 0 .0 0 2 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.00783 0.0710 0.00807 0.0426 < 0 .0 0 2 0 0 0.213 0.0651 0.00837 0.0466 0.118 0.0556 0.00769 0.0468 0.0751 0.0325 < 0 .0 0 5 0 0 0.0316 0.0413 0.0362 0.00517 0.0312 0.0477 0.0269 < 0 .0 0 5 0 0 0.0242 0.0356 0.0190 < 0 .0 0 5 0 0 0.0202 0.0276 0.0140 < 0 .0 0 5 0 0 0.0165 0.0172 0.0106 < 0 .0 0 5 0 0 0.0134 0.0126 0.0177 0.0130 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 0.0176 0.0152 0.0200 0.0322 0.0125 < 0 .0 0 5 0 0 0.0154 0.0314 0.0132 < 0 .0 0 5 0 0 0.0159 0.0348 0.0143 < 0 .0 0 5 0 0 0.0166 0.0350 0.0129 < 0 .0 0 5 0 0 0.0154 0.0350 0.0121 < 0 .0 0 5 0 0 0.0121 0.0282 0.0115 < 0 .0 0 5 0 0 0.0141 0.0270 0.0112 < 0 .0 0 5 0 0 0.0133 0.0257 0.00928 < 0 .0 0 5 0 0 0.0115 0.0189 0.0111 < 0 .0 0 5 0 0 0.0123 0.0141 FOSA 0.00569 0.00661 0.00258 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00617 0.00506 0.00306 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 0.00477 0.00286 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 N-MeFOSAA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 N-EtFOSAA < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 Page 2 of 3 Sampling Reach No. T9 Location Number 01 02 03 04 05 06 07 08 09 10 Sample ID D A L-S W -T901-0-121205 D A L-S W -T902-0-121205 D A L-S W -T903-0-121205 D A L-S W -T904-0-121205 D A L-S W -T905-0-121205 D A L-S W -T906-0-121205 D A L-S W -T907-0-121205 D A L-S W -T908-0-121205 D A L-S W -T909-0-121205 D A L-S W -T910-0-121205 Date 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 1 2 /5 /2 0 1 2 Time 10:40 11:10 11:15 11:30 11:45 12:00 12:10 12:30 13:10 13:10 Table 3-7 Surface Water PFC Data - Transect Locations Analytical Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory PFBA 0.00776 0.00951 0.00745 0.00898 0.00748 0.00676 0.00769 0.00674 0.00866 0.00796 PFHxA 0.00909 0.00772 0.00762 0.00782 0.00878 0.00657 0.00725 0.00658 0.00712 0.00642 PFHpA 0.00332 0.00388 0.00216 0.00328 0.00311 0.00330 0.00399 0.00343 0.00337 0.00337 PFOA 0.0129 0.0119 0.0119 0.0131 0.0132 0.0109 0.0115 0.0121 0.0116 0.0109 Notes: PFC concentrations are the average of the primary and duplicate analytical results expressed in in ng/mL (ppb) -- Not Analyzed PFOS = Perfluorooctane sulfonate PFBA = Perfluorobutanoic acid PFHxA = Perfluorohexanoic acid PFHpA = Perfluoroheptanoic acid PFOA = Perfluorooctanoic acid PFBS = Perfluorobutane sulfonate PFHS = Perfluorohexane sulfonate FBSA = Perfluorobutanesulfonamide FOSA =Perfluorooctanesulfonamide N-MeFOSAA = 2-(N-ethyl-PFOSA) acetic acid N-EtFOSAA = 2-(N-methyl-PFOSA) acetic acid PFC Concentrations (ng/mL ; ppb) PFBS PFHS PFOS FBSA 0.0177 < 0 .0 0 5 0 0 0.0203 0.0347 0.0112 < 0 .0 0 5 0 0 0.0205 0.0351 0.0142 < 0 .0 0 5 0 0 0.0201 0.0334 0.0137 < 0 .0 0 5 0 0 0.0207 0.0369 0.0147 < 0 .0 0 5 0 0 0.0244 0.0354 0.0139 < 0 .0 0 5 0 0 0.0197 0.0432 0.0127 < 0 .0 0 5 0 0 0.0214 0.0439 0.0134 < 0 .0 0 5 0 0 0.0203 0.0454 0.0128 < 0 .0 0 5 0 0 0.0192 0.0463 0.0134 < 0 .0 0 5 0 0 0.0190 0.0455 FOSA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 N-MeFOSAA < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 < 0 .0 0 2 0 0 N-EtFOSAA < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 < 0 .0 0 5 0 0 Page 3 of 3 S am p le ID Date MSF071 MSF073D (MSF071) MSF087 MSF088D (MSF087) MSF109 MSF110D (MSF109) MSF119 MSF121D (MSF119) MSF092 MSF093D (MSF092) MSF170 MSF171D (MSF170) MSF125 MSF126D (MSF125) MSF130 MSF132D (MSF130) MSF141 MSF143D (MSF141) MSF004 MSF005D (MSF004) MSF006 MSF008D (MSF006) MSF030 MSF032D (MSF030) MSF037 MSF038D (MSF037) MSF042 MSF043D (MSF042) MSF027 MSF028D (MSF027) MSF048 MSF050D (MSF048) MSF052 MSF054D (MSF052) MSF054D MSF062 MSF064D (MSF062) 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/29/2012 11/29/2012 11/16/2012 11/16/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 IPF070 IPF072D (IPF070) IPF113 IPF114D (IPF113) IPF101 IPF103D (IPF101) IPF161 IPF162D (IPF161) IPF148 IPF150D (IPF148) IPF135 IPF136D (IPF135) IPF014 IPF016D (IPF014) IPF153 IPF154D (IPF153) IPF155 IPF156D (IPF155) IPF018 IPF019D (IPF018) IPF176 IPF177D (IPF176) IPF057 IPF059D (IPF057) 11/9/2012 11/9/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/29/2012 11/29/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/29/2012 11/29/2012 11/7/2012 11/7/2012 T able 3-8 Interlaboratory Analytical C om parison - PFOS 3M E nvironm ental L aboratory AXYS Analytical Fall 2012 Fish an d S u rfa ce W ater S am pling Decatur, Alabama Analytical Laboratory 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental AXYS Laboratory 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory AXYS Laboratory 3M Environmental AXYS Laboratory Reach Number Reach Name Largem outh B ass Fish Tissue R01 RM 320 R01 RM 320 R02 Point Mallard R02 Point Mallard R03 RM307.5 (Backwater) R03 RM307.5 (Backwater) R03 RM307.5 (Backwater) R03 RM307.5 (Backwater) R04 Betty Rye Creek & Dry Branch R04 Betty Rye Creek & Dry Branch R05 Bakers Creek R05 Bakers Creek R06 Swan Creek R06 Swan Creek R07 River Mile 298 R07 River Mile 298 R08 Round Island Creek R08 Round Island Creek R09 Fox Creek R09 Fox Creek R09 Fox Creek R09 Fox Creek R10 Browns Ferry R10 Browns Ferry R10 Browns Ferry R10 Browns Ferry R11 Mallard Creek R11 Mallard Creek R12 Elk River R12 Elk River R14 Big Nance Creek R14 Big Nance Creek R14 Big Nance Creek R14 Big Nance Creek R14 Big Nance Creek R15 Shoal Creek R15 Shoal Creek 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental AXYS Laboratory 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental Channel Catfish Fish T issue R01 RM 320 R01 RM 320 R02 Point Mallard R02 Point Mallard R04 Betty Rye Creek & Dry Branch R04 Betty Rye Creek & Dry Branch R05 Bakers Creek R05 Bakers Creek R07 River Mile 298 R07 River Mile 298 R08 Round Island Creek R08 Round Island Creek R09 Fox Creek R09 Fox Creek R10 Browns Ferry R10 Browns Ferry R11 Mallard Creek R11 Mallard Creek R12 Elk River R12 Elk River R13 River Mile 281 R13 River Mile 281 R15 Shoal Creek R15 Shoal Creek River Mile RM320 RM320 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM303 RM303 RM301 RM301 RM301.2 RM301.2 RM298 RM298 RM298 RM298 RM296 RM296 RM296 RM296 RM294 RM294 RM294 RM294 RM293.5 RM293.5 RM285 RM285 RM273 RM273 RM273 RM273 RM273 RM264 RM264 RM320 RM320 RM307.5 RM307.5 RM303 RM303 RM301 RM301 RM298 RM298 RM298 RM298 RM296 RM296 RM294 RM294 RM293.5 RM293.5 RM285 RM285 RM281 RM281 RM264 RM264 M inimum RPD M axim um RPD A verage RPD Number of sam ples FIST FILET SUMMARY STATISTICS 3M - 3M 3M - AXYS 0.6 0.5 19.2 18.5 6.2 8.7 15 15 AXYS-AXYS 11.6 11.6 11.6 1 PFOS C oncentration (ppb) Relative Percent Difference 35.8 36.3 49.0 56.6 21.8 22 23.9 19.9 434 432 373 345 40.8 [a] 43.9 333 324 134 143 167 168 173 186 65.2 56.1 101.9 106 210 [b] 224 65.3 65.9 88.9 81.6 93.5 [b] 91.7 103 103 99.8 A verage RPD: 1.81 1.66 1.30 1.24 50.9 42.0 85.6 79.3 69.6 57.8 4.89 [b] 4.12 14.3 14.6 16.3 14.5 11.9 12.3 11.7 10.2 11.8 12.7 7.33 7.56 A verage RPD: 1.4 14.4 0.9 18.3 0.5 7.8 7.3 2.7 6.5 0.6 7.2 15.0 3.9 6.5 0.9 8.6 1.9 11.6 3.2 6.3 8.6 4.7 19.2 7.6 18.5 17.1 2.1 11.7 3.3 13.7 7.3 3.1 9.7 S am p le ID T1 - 01 T2 - 02 T3 - 03 T4 - 04 T5 - 05 T6 - 06 T7 - 07 T8 - 08 T9 - 09 R02 - L3 R05 - L1 R06 - L2 R10 - L3 R15 - L1 Date 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 11/16/2012 11/16/2012 11/16/2012 11/19/2012 11/19/2012 11/19/2012 11/20/2012 11/20/2012 11/19/2012 11/19/2012 11/19/2012 11/17/2012 11/17/2012 11/17/2012 T able 3-8 Interlaboratory Analytical C om parison - PFOS 3M E nvironm ental L aboratory AXYS Analytical Fall 2012 Fish an d S u rfa ce W ater S am pling Decatur, Alabama Analytical Laboratory R each Num ber Reach Name River Mile 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory Surface W ater - S urface W ater T ransects ------- ------- ---- -------- ------- - - - - - 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory S urface W ater - Surface W ater Fish R eaches --------------- - SURFACE WATER SUMMARY STATISTICS 3M - 3M 3M - AXYS M inimum RPD 1.0 0.8 M axim um RPD 14.9 34.1 A verage RPD 6.3 13.9 Number of sam ples 14 14 AXYS-AXYS 14.6 14.6 14.6 1 N o tes: Average PFO S concentrations for fish tissue are the average of the primary and duplicate analytical results expressed in ng/g (ppb) on a wet weight basis. Average PFO S concentrations for surface water are the average of primary and duplicate analytical results in ngm/L (ppb). [a] Value reported for single result, duplicate sample analysis had peak shift out of retention time window and no result determined. [b] RPD > 20% PFOS C oncentration (ppb) Relative Percent Difference 0.00613 0.00636 0.00526 0.00993 0.00943 0.00844 0.00694 0.00751 0.00668 0.00729 0.00846 0.00630 0.00720 0.00678 0.00510 0.00972 0.0111 0.00745 0.00862 0.00202 0.0193 0.0173 0.0133 0.0143 0.0128 0.0192 0.0194 0.0177 A verage RPD: 0.00894 0.00846 0.00759 0.385 0.405 0.388 0.00672 0.00766 0.00649 0.00663 0.00548 0.0199 0.0189 0.0220 A verage RPD: 3.7 15.3 5.2 16.2 7.9 3.8 14.9 14.6 6.0 34.1 13.3 26.4 14.6 4.6 15.5 7.2 3.8 1.0 8.1 11.4 5.5 16.3 5.1 0.8 13.1 2.1 16.9 5.2 10.0 8.3 4. DATA EVALUATION Data generated during the fall 2012 fish and surface water sampling program and previous studies in the Tennessee River were evaluated to identify spatial and temporal trends in fish tissue and surface water PFOS concentrations. Qualitative evaluations were performed on fish and surface water data sets insufficient for quantitative statistical evaluation because of confounding factors, small sample size, and/or spatial coverage limitations. Graphical techniques were primarily used such as time series bar graphs and box plots by individual sampling location to show temporal trends and bar graphs and box plots of concentrations for multiple locations during individual sampling events vs. distance (river mile; RM) to show spatial variations. A typical box plot with definitions is provided as Figure 2-2. Quantitative evaluation of temporal trends in the fish and surface water data sets that support formal statistical testing included trend analysis for locations with sufficiently large data sets (i.e., greater than or equal to 4 sampling events) using the nonparametric Mann-Kendall trend test to determine whether media concentrations have changed over time. Tests for differences in median concentrations between locations/areas were performed using the nonparametric Mann-Whitney (=Wilcoxon Rank Sum; WRS) test on fish data sets with five or more sample results per location in each sampling event. Mann-Whitney tests were performed for spatial evaluation (i.e., evaluating whether median concentrations at one location are statistically significantly different than median concentrations at another location during the same time period) as well as temporal evaluations (i.e., evaluating whether median concentrations are statistically significantly different between two time periods at the same sampling location). Data for the spatial/temporal analysis are introduced and described in the following fish and surface water subsections. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 4-1 4.1 EVALUATION OF FISH SAMPLING RESULTS 4.1.1 Historical Data Between 2002 and 2012, eight fish sampling events have been performed in the Tennessee River in the vicinity of the 3M Decatur facility with analysis of fish filet tissue samples for PFOS concentrations. These eight events are summarized below: (1) April 2002 Entrix - A total of 38 fish specimens, including 18 largemouth bass, 19 channel catfish, and 1 smallmouth bass (Micropterus dolomieu), were collected across three sampling reaches between April 15 and April 18, 2002. The sampling locations in this field event were downstream of the 3M Decatur facility at Fox Creek (approximately RM296), across the river from the 3M Decatur facility (approximately RM301.2 at the mouth of Swan Creek) and upstream of the facility near Point Mallard (approximately RM307.5). All samples were prepared as skin-off filets (Entrix, 2003). (2) December 2004 WESTON - Fish sampling was performed at six locations (reaches) in the Tennessee River and Bakers Creek from October through December 2004. A total of 120 fish specimens, including 60 largemouth bass and 60 channel catfish, were collected across six sampling reaches with 10 largemouth bass samples and 10 channel catfish samples at each of the six reaches. The samples were collected from the three LOI locations, Bakers Creek (DBC), a facility outfall cove sampling area (DOU), and Mallard Creek (DMC). O f the 10 samples per species, at each reach, five were prepared for skin-on filet tissue samples and five were prepared for whole body samples (WESTON, 2008). (3) December 2006 WESTON - Fish sampling was performed at the same six reaches in the Tennessee River and Bakers Creek as the 2004 sampling event. A total of 112 fish samples were collected, including 10 samples of largemouth bass and 10 samples of channel catfish at each of the sampling reaches. O f these 10 samples per species, five were prepared for skin-on filet tissue samples and five were prepared for whole body samples. At the cross river location, only two J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 ReportYDraft Final Fall 2012 Fish and Surface Water Report.doc 4-2 largemouth bass samples were collected, and both were designated for whole body analysis (WESTON, 2008). (4) 2007 ADEM - A total of 48 fish specimens, including 24 largemouth bass and 24 channel catfish, were collected from the Tennessee River by ADEM in 2007. Six largemouth bass and six channel catfish were collected at each of four sampling locations: (1) Wheeler Lake at approximately RM281 (TENR-281), (2) upstream of the I-65 bridge at approximately RM320 (TENR-320), (3) near the 3M WWTP outfall at approximately RM300 (TENR-300), and (4) near Bakers Creek (WHEL-11). All samples were prepared as skin-off filets. (5) 2008 ADEM - A total of 60 fish specimens, including 30 largemouth bass and 30 channel catfish, were collected by ADEM in 2008. Six largemouth bass and six channel catfish were collected at each of the five sampling locations: (1) upstream on Flint Creek (approximately 9 miles upstream of the confluence with the Tennessee River) [FTCM-6], (2) Wheeler Lake at approximately RM281 (TENR281), (3) near the 3M WWTP outfall at approximately RM300 (TENR-300), (4) upstream of the I-65 bridge at approximately RM320 (TENR-320), and (5) near Bakers Creek (WHEL-11). All samples were prepared as skin-off filets. Data from the FTCM-6 samples were not used for comparisons with results of sampling in the main river study area.6 (6) 2009 ADEM - A total o f 102 fish specimens, including 48 largemouth bass and 42 channel catfish, were collected by ADEM in 2009. An additional six golden redhorse and six smallmouth buffalo were collected but were not included in this analysis. Six largemouth bass and six channel catfish were collected at seven of the eight sampling locations, whereas only six largemouth bass were collected at one sampling location (BGNL-1). The eight sampling locations were: (1) upstream of the mouth of Flint Creek (WHEL-6), (2) upstream on Flint Creek (approximately 9 miles upstream of the confluence with the Tennessee River) [FTCM-6], (3) Wheeler Lake at approximately RM281 (TENR-281), (4) near the 3M WWTP outfall at approximately RM300 (TENR-300), (5) upstream of the J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 4-3 I-65 bridge at approximately RM320 (TENR-320), (6) near Bakers Creek (WHEL-11), (7) upstream of the mouth of Big Nance Creek (WILL-1), and (8) upstream on Big Nance Creek (BNGL-1). All samples were prepared as skin-off filets. Only the TENR-281, TENR-300, W HEL-11 and TENR-320 locations were used for comparison with fish filet PFOS concentration data from other sampling events in the study area. (7) April 2012 WESTON - Fish sampling was performed at three adjoining reaches in the vicinity of the mouth of Bakers Creek on April 3 to 6, 2012. A total of 26 largemouth bass were collected across the three sampling reaches with 10 largemouth bass samples from the upper reach immediately above the mouth of Bakers Creek, 10 largemouth bass samples from the mouth o f Bakers Creek and six largemouth bass samples from the reach immediately below the mouth of Bakers Creek. All samples were prepared as skin-off filets. (8) November/December 2012 WESTON - As described in Subsection 3.1, a total of 150 fish specimens, including 75 largemouth bass and 75 catfish, were collected in the fall 2012 sampling event. These specimens included five largemouth bass and five channel catfish at each of the 15 fish sampling reaches. All samples were prepared as skin-off filets. An initial fish sampling event was performed in June 2000 by ENTRIX. However, the sample types collected were whole body fish samples and fish liver samples. These sample types are inconsistent with filet samples collected during subsequent sampling events and therefore were not considered in this evaluation. Fish and surface water data from past studies were aggregated by reach/river mile for comparison with current study data. For example, data developed by 3M from the mouth of Bakers Creek and the DOU locations were combined with the ADEM TENR-300 location as RM301 to allow for the evaluation of spatial and temporal trends. There are several sources of uncertainty associated with comparisons between past and present data sets. These include the accuracy o f location information, improvements in analytical J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 ReportYDraf Final Fall 2012 Fish and Surface Water Report.doc 4-4 methods over time, differing tissue sample types (i.e., skin-on filet samples from 2004 and 2006 vs. skin-off filet samples from ADEM (2007 to 2009) and the April and November 2012 collections), and differences in surface water sampling methods such as the collection of discrete water samples from the near surface versus composite samples intended to characterize the full water column. The magnitude and direction of any biases introduced from these variables is unknown but is not expected to invalidate comparisons between locations and years or significantly alter any trends observed. 4.1.2 Graphical Examination Box plots were generated from largemouth bass and catfish filet tissue PFOS data by sampling location (river mile) for each of the sampling events to illustrate temporal changes and by event for each of the sampling locations to illustrate spatial variation. Largemouth bass box plots for each event at RM320, RM307.5 (Point Mallard), RM301 (Bakers Creek), RM296 (Fox Creek), RM293.5 (Mallard Creek mouth) and RM281 are shown in Figures 4-1 through 4-6, respectively. Largemouth bass box plots for each of the locations sampled during the November 2004, December 2006 and November 2012 sampling events are shown in Figures 4-7 through 4-9, respectively. A typical box plot with definitions is provided as Figure 2-2. Channel catfish box plots illustrating changes over time at the RM320, RM307.5, RM301, RM296, RM293.5 and RM281 locations are shown in Figures 4-10 through 4-15, respectively. Channel catfish box plots for each of the sampling locations associated with the November 2004, December 2006 and November 2012 sampling events are shown in Figures 4-16 through 4-18. A complete set of largemouth bass and channel catfish box plots are provided in Appendix F. These combination box plots allow for qualitative comparisons of sample median values and ranges between different data sets and provide a visual representation of spatial or temporal trends in PFOS concentrations. The significance of observable differences between locations and within locations over time is provided in the quantitative statistical analysis in the following subsections. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draf Final Fall 2012 Fish and Surface Water Report.doc 4-5 4.1.3 Statistical Analysis 4.1.3.1 Temporal Evaluation Mann-Kendall TrendAnalysis Sampling locations with PFOS concentration data for tissue samples from four or more sampling events were RM281 (below Spring Creek), RM296 (Fox Creek mouth; largemouth bass only), RM301 (Bakers Creek mouth), RM307.5 (Point Mallard) and RM320 (below Triana, AL). The results of Mann-Kendall tests for trend performed on these largemouth bass and channel catfish filet tissue PFOS data sets at a significance level of a=0.05 are provided in Table 4-1. Statistically significant decreasing trends were identified for largemouth bass PFOS concentrations at all locations except for Point Mallard at RM307.5 where no statistically significant trend was identified. Sample median concentrations at upstream locations RM307.5 and RM320 are an order of magnitude lower than the downstream reaches (i.e., RM301, RM296 and RM281). Statistically significant decreasing trends were identified for channel catfish PFOS concentrations at the Point Mallard (RM307.5) and Bakers Creek mouth (RM301) locations. An increasing trend was identified for the RM320 location (below Triana) channel catfish PFOS concentrations, and no statistically significant trend was evident at RM281 (below Spring Creek). The sample median concentration at the upstream reaches (RM320 and RM307.5) is two orders of magnitude lower than the Bakers Creek (RM301). The sample median concentration at Reach 281 is an order of magnitude lower than Bakers Creek (RM301). Mann-Whitney Tests In addition to Mann-Kendall trend analysis, the Mann-Whitney test was performed at locations with filet tissue data spanning multiple sampling events to test for statistically significant differences in median concentrations over time. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 4-6 The results of Mann-Whitney tests performed on these largemouth bass and channel catfish filet tissue PFOS data sets at a significance level of a=0.05 are provided in Table 4-2 and Table 4-3, respectively, and are summarized below. Graphical presentations of sample median filet tissue concentrations for the largemouth bass and channel catfish data at specific sampling reaches for the various sampling events are provided in the box plots in Figures 4-1 through 4-6 and Figures 4-10 through 4-15 for the largemouth bass and channel catfish, respectively. Largemouth Bass Statistically significant declines in median filet PFOS concentrations were identified between the first sampling event and the most recent sampling event (November 2012) at the following reaches: Reach 01 (RM320), Reach 02 (Point Mallard at RM307.5), Reach 05 (Bakers Creek mouth at RM301), Reach 09 (Fox Creek mouth at RM296) and Reach 13 (below Spring Creek at RM281). The only two sampling reaches with sufficient data for analysis with no significant decline between the first sampling event and the most recent sampling event were Reach 06 (Swan Creek mouth at RM301.2) and Reach 11 (Mallard Creek mouth at RM293.5). Reach 06 (RM301.2) is located along the northern shoreline of the river where no statistically significant differences in median PFOS concentration were identified between the three sampling events in June 2002, November 2004 and November 2012, with sample median values ranging from 50.6 to 72.4 ng/g (ppb). Reach 11 (Mallard Creek mouth; RM293.5) is located along the southern shoreline downstream of the industrialized area in Decatur. Although no statistically significant decrease in median PFOS concentration was identified between the first sampling event (November 2004) and the most recent sampling event (November 2012) at Reach 11, a statistically significant decline in median filet PFOS concentration was identified between the two most recent sampling events (December 2006 and November 2012). Examination of the initial 2004 data set for the reach at the mouth of Mallard Creek indicates high variability and a median value that likely underestimated the central tendency of the fish tissue concentrations in 2004 (see Figure 4-5). J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 4-7 Channel Catfish Statistically significant declines in median channel catfish filet PFOS concentrations were identified between the first sampling event (initial date dependent on sampling reach) and the most recent sampling event (November 2012) at Reach 05 (Bakers Creek mouth at RM301), Reach 06 (Swan Creek Mouth at RM301.2) and Reach 09 (Fox Creek mouth at RM296). No statistically significant temporal trends in median concentrations were identified at Reach 01 (RM320) or Reach 11 (RM293.5). No trends were identified relative to the most recent sampling event at Reach 13 (RM281). A statistically significant decline in median concentration was identified at Reach 02 (Point Mallard Creek Mouth at RM307.5) between the two most recent sampling events (December 2006 and November 2012). 4.1.3.2 Spatial Evaluation The fish tissue data from the fall 2012 sampling event covered a 56 river mile portion of the Tennessee River and allowed for broader spatial analysis than previously possible. The Mann-Whitney data analysis identified statistically significant differences in median fish tissue PFOS concentrations in both largemouth bass and channel catfish samples from adjacent fish sampling reaches in the river. The outcome of Mann-Whitney testing and the sample median values are presented in Table 4-4 for the largemouth bass and Table 4-5 for the channel catfish. Graphical presentations of sample median filet tissue concentrations for the largemouth bass and channel catfish data in each of the sampling reaches are provided in the box plots in Figures 4-9 and 4-18. Largemouth Bass In the upstream reaches (Reaches 01 through 03; RM320 through RM307.5), a statistically significant difference was found between median filet PFOS tissue concentrations in Reaches 01 and 02. No statistically significant difference was found between Reaches 01 and 03 or between Reaches 02 and 03. W ith the exception of a comparison of Reach 06 and Reach 02, all of the downstream comparisons of Reaches 04 J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 4-8 through 15 against Reaches 01, 02 and 03 are significantly different (i.e., higher downstream median concentrations). Among the consecutive reaches along the industrialized southern shoreline in the vicinity of Decatur and immediately downstream, no significant differences in median filet PFOS concentrations were identified between Reach 04 (Betty Rye Creek and Dry Branch at RM303) and Reach 05 (Bakers Creek mouth at RM301), Reach 05 and Reach 07 (RM298), and Reach 07 and Reach 09 (Fox Creek mouth at RM296). A statistically significantly different decrease in median PFOS concentrations was identified at Reach 11 (Mallard Creek mouth at RM293.5) relative to the upstream Reach 09 (Fox Creek mouth at RM296). No statistically significant differences in median filet PFOS concentrations were found between Reach 06 (Swan Creek; RM301.2) and Reach 08 (Round Island Creek; RM298) or between Reach 08 (RM298) and 10 (Browns Ferry; RM294), which span the north shoreline of the Tennessee River. Median filet PFOS concentrations at the reaches on the southern shoreline were statistically significantly higher than the median concentrations at adjacent reaches along the northern shoreline of the Tennessee River. The first location downstream below the industrialized southern shoreline in the vicinity of Decatur where median filet PFOS concentrations along the southern shoreline were not significantly different from the adjacent reach along the northern shoreline was observed in the comparison of Reach 10 (Browns Ferry; RM294) and Reach 11 (Mallard Creek; RM293.5). As described above, Mallard Creek mouth is also the first location downstream of Decatur where the median PFOS concentration was significantly lower than the median at the adjacent upstream location along the southern shoreline (Fox Creek mouth; RM296). Farther downstream, no statistically significant differences in median filet PFOS concentrations were detected between the farthest downstream sampling reach in Wheeler Reservoir (Reach 13; RM281) and the farthest upstream reach in Wilson J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 4-9 Reservoir (Reach 14; RM273) or between the two Wilson Reservoir sampling locations (Reaches 14 and 15). Channel Catfish In the upstream reaches (Reaches 01 through 03; RM320 through RM307.5), no statistically significant differences were found between the median channel catfish filet PFOS concentrations between Reaches 01, 02 and 03. W ith the exception of the comparison between Reach 06 and Reach 01 and between Reach 06 and Reach 03, all of the downstream comparisons of Reaches 04 through 15 against Reach 01, 02 and 03 are statistically significantly different (i.e., higher median PFOS concentrations in the downstream reaches). The median filet PFOS concentration was statistically significantly higher in Reach 05 (Bakers Creek mouth at RM301) than the median concentration associated with the adjacent upstream Reach 04 (Betty Rye Creek and Dry Branch at RM303). Downstream from Reach 05 along the southern shoreline of the Tennessee River, the median PFOS concentration at Reach 07 (RM298) was statistically significantly lower than the median at Reach 05. Similar to the largemouth bass filet PFOS concentration, the catfish median filet PFOS concentration at Reach 06 (Swan Creek; RM301.2) located on the northern shoreline was statistically significantly lower than the median filet PFOS concentrations at Reaches 04 and 05. Along the northern shoreline in the vicinity of Decatur, no significant differences in median PFOS filet concentrations were found between Reach 06 (RM301.2) and Reach 08 (RM298), Reach 08 and Reach 10 (RM294) or Reach 10 and Reach 12 (RM285), which are adjacent reaches spanning the northern shoreline of the Tennessee River. The reaches on the southern shoreline had statistically significantly higher median filet PFOS concentrations than the adjacent reaches located on the northern shoreline of the Tennessee River. The first instance below the facility where channel catfish filet median PFOS concentrations were not significantly different between the northern and southern J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 4-10 shoreline locations was between Reach 09 (Fox Creek mouth; RM296) and Reach 10 (Browns Ferry; RM294). Reach 09 is immediately downstream and adjacent to Reach 07 (RM298), the first instance downstream along the southern shoreline where the median PFOS concentration was statistically significantly lower than the immediate upstream location (Reach 05). No statistically significant difference between median filet PFOS concentrations was identified between Reach 07 (RM298) and Reach 09 (Fox Creek mouth; RM296) or between Reach 09 and Reach 11 (Mallard Creek Mouth; RM293.5) on the southern shoreline of the river. Farther downstream in W heeler Reservoir, median PFOS concentrations at Reach 12 at the Elk River mouth (RM285) on the northern shoreline and Reach 13 below Spring Creek (RM281) on the southern shoreline were statistically significantly lower than Reach 11 at the Mallard Creek mouth (RM293.5). Farther downstream, no significant difference in median filet PFOS concentrations were detected between the farthest downstream sampling reach in W heeler Reservoir (Reach 13; RM281) and the farthest upstream reach in Wilson Reservoir (Reach 14; RM273) or between the two Wilson Reservoir locations (Reaches 14 and 15). 4.2 EVALUATION OF SURFACE WATER SAMPLING RESULTS 4.2.1 Historical Data Since 2000, there have been eight separate surface water sampling events for PFOS in the Tennessee River in the vicinity of the 3M Decatur facility. These eight events are summarized below: (1) June 2000 Entrix - Twenty-six surface water samples were collected from five locations in the Tennessee River from June 19 to 21, 2000, and one sample was collected at the 3M outfall. The five locations ranged between a location upstream of the Guntersville Dam at RM370 and the mouth of Fox Creek at RM296 (Entrix, 2001). J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 ReportYDraft Final Fall 2012 Fish and Surface Water Report.doc 4-11 (2) November 2000 - A total of 40 surface water samples were collected between November 14 and 16, 2000 across approximately 80 river miles of the Tennessee River between RM337 and RM261, and the results were published in the Journal o fEnvironmental Science and Technology (Hansen et al., 2002). (3) April 2002 ENTRIX - Three grab samples of surface water were collected on April 15, 2002 at each of three locations on the Tennessee River; downstream of the 3M Decatur facility at Fox Creek (approximately RM296), across the river from the 3M Decatur facility (approximately RM301) and upstream of the facility near the Highway 31 bridge (approximately RM307.5). These sampling locations are the three LOI locations (Entrix, 2003). (4) December 2004 WESTON - A surface water sample was collected from each of eight sampling locations along the Tennessee River between RM293.5 and RM307.5 on December 1 to 2, 2004. The eight locations sampled included the three LOI sampling locations, the mouth of Mallard Creek (approximately RM293.5; DMC), the cove and mouth of Bakers Creek (approximately RM301; DOU), Bakers Creek (approximately RM301; DBC), and location DDO downstream of the Daikin wastewater treatment effluent outfall (WESTON, 2008). (5) July 2005 WESTON - A longitudinal series of surface water sampling was performed along 53 miles of the Tennessee River and at three transects across the width of the river channel in July 2005. The longitudinal series consisted of 11 samples collected between RM207 and RM254. The three transects were located near the 3M Decatur facility at approximately RM301, RM299 and RM287, and consisted of 10 equally spaced sampling stations across the river (WESTON, 2008).6 (6) December 2006 WESTON - One surface water sample was collected from each of six sampling locations along the Tennessee River on December 13-15, 2006. Surface water sampling was performed at the same six reaches in the Tennessee J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 ReportYDraft Final Fall 2012 Fish and Surface Water Report.doc 4-12 River and Bakers Creek as the 2004 sampling event and included the three LOI locations DMC, DOU and DBC; at the mouth of Mallard Creek; along the 3M facility shoreline and in the mouth of Bakers Creek, respectively (WESTON, 2008). (7) April 2012 WESTON - One set of water samples was collected on April 5, 2012 and consisted of three surface water samples, one water sample in each of the three sampling reaches: upstream of Bakers Creek, in the mouth of Bakers Creek, and immediately downstream of Bakers Creek (WESTON, 2012b). (8) November - December 2012 WESTON - As described in Subsection 3.2, a total of 135 surface water samples, excluding duplicates, were collected over approximately 56 miles of the Tennessee River in November/December 2012 by WESTON. These samples included three surface water samples collected at each of the 15 fish sampling reaches in November 2012 and 10 surface water samples collected across each of nine surface water transects. Sampling locations were selected to include areas with historical data on PFOS concentrations. 4.2.2 Graphical Examination Time series three-dimensional bar graphs were generated from surface water PFOS data by sampling location (river mile) for each of the sampling events to illustrate temporal changes and by event for each of the sampling locations to illustrate spatial variation. The three-dimensional bar graph was prepared for the three surface water reaches with four or more sampling events (i.e., Point Mallard (RM307.5), Bakers Creek (RM301) and Fox Creek (RM296). These time series plots are shown in Figure 4-19 and Figure 4-20. Visual interpretation of the bar graphs indicates a decreasing trend at each of these locations between 2000 and 2012. A three-dimensional bar graph was also generated from surface water PFOS data by sampling location (river mile) for the fall 2012 sampling event to illustrate spatial variation. Figure 4-21 depicts the surface water PFOS data from the fish sampling J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 ReportYDraft Final Fall 2012 Fish and Surface Water Report.doc 4-13 reaches and Figure 4-22 depicts the surface water PFOS data from the surface water transects. The surface water PFOS concentrations from the fall 2012 sampling in Wheeler and Wilson Reservoirs of the Tennessee River indicate that the principal sources of PFOS enter the river along the southern shoreline of the Tennessee River within the industrialized area at and below the City of Decatur and upstream at Flint Creek with bank-attached flow and relatively slow mixing across the river. Upstream of Decatur, the surface water PFOS concentrations were less than 0.01 ng/mL (ppb) with little variation across the river. The highest surface water PFOS concentrations were located between RM303 and RM293.5, which corresponds with the reach of river impacted by sources along the industrialized southern shoreline in the vicinity o f Decatur. As depicted in Figures 4-21 and 4-22, PFOS is more uniformly distributed across the river and decreases in magnitude downstream from this area. Based on the similarities in surface water PFOS concentrations across transect number 08, complete mixing across the width of the river appears to occur between the mouth of Mallard Creek (Reach 11; RM293.5) and below Spring Creek (Reach 13; RM281), see Figures 3-6 and 4-22. In addition to the industrial shoreline in the vicinity of Decatur (which includes the area of the 3M Decatur facility, Daikin and the Decatur Utilities wastewater treatment plant) potential PFOS sources were identified in areas of Wheeler Reservoir, including the confluences of Flint Creek, Betty Rye Creek and Dry Branch, and Fox Creek with the Tennessee River. Potential source areas were identified where PFOS concentrations were observed at higher concentrations than the corresponding upstream concentrations and, particularly, at the sampling locations closest to the shore, relative to the cross stream concentrations (see Figures 3-5, 3-6, 4-21 and 4-22). PFOS sample median concentrations were approximately 0.006 ng/mL greater in the surface water transect (T9) in Wilson Reservoir, with a median concentration of 0.0203 ng/mL compared to the median concentration for the farthest downstream surface water transect in Wheeler Reservoir (T8) of 0.0147 ng/mL. This change in concentrations J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 4-14 indicates potential additional PFOS sources entering the Tennessee River in the general vicinity of the Wheeler Dam. 4.2.3 Statistical Analysis Consistent with the temporal evaluation of fish tissue results described in Subsection 4.1.3, Mann-Kendall trend tests were also performed on surface water PFOS data sets with four or more sampling events over time. As shown in Table 4-6, statistically significant decreasing median PFOS surface water concentrations were identified at the three locations with sufficient data to perform the analysis: Point Mallard (RM307.5), Bakers Creek mouth (RM301) and Fox Creek mouth (RM296). These three locations span stretches of the Tennessee River within or above the industrialized southern shoreline in the immediate vicinity of Decatur. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 4-15 Figure 4-1 Largemouth Bass PFOS Box Plots for the River Mile 320 (Reach 01) Below Triana, AL Location 2007 to 2012 Figure 4-2 Largemouth Bass PFOS Box Plots for the River Mile 307.5 (Reach 02) Point Mallard Location 2004 to 2012 Figure 4-3 Largemouth Bass PFOS Box Plots for the River Mile 301 (Reach 05) Bakers Creek Mouth Location 2004 to 2012 Figure 4-4 Largemouth Bass PFOS Box Plots for the River Mile 296 (Reach 09) Fox Creek Mouth Location 2002 to 2012 Figure 4-5 Largemouth Bass PFOS Box Plots for the River Mile 293.5 (Reach 11) Mallard Creek Mouth Location 2004 to 2012 Figure 4-6 Largemouth Bass PFOS Box Plots for the River Mile 281 (Reach 13) Location 2007 to 2012 Figure 4-7 Largemouth Bass PFOS Box Plots for the 2004 Sampling Locations Legend: = S am p le reach located on the north bank of the T e n n e s s e e R iver = S am p le reach located on the south bank of the T e n n e s s e e R iver Figure 4-8 Largemouth Bass PFOS Box Plots for the 2006 Sampling Locations qdd s o d d sseg Lunotti allien Figure 4-9 Largemouth Bass PFOS Box Plots for the 2012 Sampling Locations Legend: = S am p le reach located on the north bank o f the T e n n e s s e e R iver = S am p le reach located on the south bank of the T en n essee R iver Figure 4-10 Channel Catfish PFOS Box Plots for the River Mile 320 (Reach 01) Below Triana, AL Location 2007 to 2012 Figure 4-11 Channel Catfish PFOS Box Plots for the River Mile 307.5 (Reach 02) Point Mallard Location 2002 to 2012 Figure 4-12 Channel Catfish PFOS Box Plots for the River Mile 301 (Reach 05) Bakers Creek Mouth Location 2004 to 2012 Bgx Plot of Channel_Catfish_PFOS_ppb River_Mile=301 Channel_Catfish_PFOS_ppb i Jun-02 i Nov-04 i Dec-06 i Jul-07 Date i Jul-08 i Jul-09 i Nov-12 Figure 4-13 Channel Catfish PFOS Box Plots for the River Mile 296 (Reach 09) Fox Creek Mouth Location 2002 to 2012 Figure 4-14 Channel Catfish PFOS Box Plots for the River Mile 293.5 (Reach 11) Mallard Creek Mouth Location 2004 to 2012 Figure 4-15 Channel Catfish PFOS Box Plots for the River Mile 281 (Reach 13) Location 2007 to 2012 Figure 4-16 Channel Catfish PFOS Box Plots for the 2004 Sampling Locations = Sample reach located on the south bank of the Tennessee River Figure 4-17 Channel Catfish PFOS Box Plots for the 2006 Sampling Locations Legend: = S am p le reach located on the north bank of the T e n n e s s e e R iver = Sample reach located on the south bank of the Tennessee River Figure 4-18 Channel Catfish PFOS Box Plots for the 2012 Sampling Locations = Sample reach located on the south bank of the Tennessee River Figure 4-19 Temporal Evaluation of Surface W ater Concentrations at Point Mallard (RM 307.5), Bakers Creek (RM 301) and Fox Creek (RM296) Fall 2012 59.13 2000.47 2002.29 2004.95 Sample Date (Decimal Year) 2006.95 2012.26 2012.89 60 50 -Q a. 40 a> a. Uc 30 uO tOo 20 10 0 RM307.5 (Point Mallard) RM301_(Bakers Creek) RM296 (Fox Creek) <>u Figure 4-20 Temporal Evaluation of Surface W ater Concentrations at Point Mallard (RM 307.5), Bakers Creek (RM 301) and Fox Creek (RM 296) [adjusted scale] 59.13 Fall 2012 4.57 2.96 2000.47 2002.29 2004.95 Sample Date (Decimal Year) 2006.95 Note: Scale adjusted to show details, for accurate scale see Figure 4-19 2012.26 2012.89 5 4 iI 3 I I I2 1 ((cU0 -QOQ-. u U DO oLO C 0 RM307.5 (Point Mallard) RM301_(Bakers Creek) 4 RM296 (Fox Creek) ' a>i Figure 4-21: Surface W a te r Average PFOS Concentrations a t Fish Reach Locations - Fall 2012 R07 R06 R05 R04 R03 R02 R01 R08 R09 R10 R11 R12 Fish Reaches R01 R13 R03 R14 R15 R05 R07 R09 R11 R13 R15 R02 R04 R06 R08 R10 R12 R14 Figure 4-22: Surface Water Average PFOS Concentrations (o -Q tO E EO O .+y* *O3 U tU) ... Q. ni tnoi --+; nui Q 0.14 264 281 293.5 296 298 301 303 307.5 320 River Mile Table 4-1 Mann-Kendall Test for Trend in Fish Filet Tissue PFOS Concentrations Location R M 3 2 0 (B elow Triana) R M 3 0 7 .5 (Point M allard) R M 301 (B akers C reek mouth) R M 2 9 6 (Fox C reek mouth) R M 281 (B elow Spring C reek) Largemouth Bass Filet Tissue PFOS Trend D ecreasing N ot statistically significant D ecreasing D ecreasing D ecreasing Channel Catfish Filet Tissue PFOS Trend Increasing D ecreasing D ecreasing Not calculated (<4 events) N ot statistically significant M an n -K en d all test for trend at significance level o f a = 0 .0 5 Jul-07 (65.5) Jul-08 (59.1) Jul-09 (48.5) Nov-12 (32.7) Table 4-2 Mann-Whitney Test for Significant Differences in Median PFOS Concentrations Largemouth Bass Filet Tissue Temporal Evaluation Reach 01 (RM 320) Jul-07 (65.5) Jul-08 (59.1) Jul-09 (48.5) NS NS NS S S NS Jun-02 (2.5) Nov-04 (114) Dec-06 (19.6) Nov-12 (44.1) Reach 02 (RM 307.5) Jun-02 (2.5) Nov-04 (114) S SS SS Dec-06 (19.6) NS Nov-04 (3355) Dec-06 (1127.5) Jul-07 (1090.7) July-08 (929.7) Jul-09 (680.9) Apr-12 (852) Nov-12 (435) Nov-04 (3355) Reach 05 (RM 301) Dec-06 (1127.5) Jul-07 (1090.7) July-08 (929.7) Jul-09 (680.9) Apr-12 (852) NS S NS S NS NS S S NS NS S NS NS NS NS S S NS NS NS S Jun-02 (51.3) Nov-04 (72.4) Nov-12 (50.6) Jun-02 (629) Nov-04 (432) Dec-06 (296) Nov-12 (259) Nov-04 (147) Dec-06 (295) Nov-12 (145) Jul-07 (139.6) Jul-08 (82.7) Jul-09 (138.4) Nov-12 (71) Reach 06 (RM 301.2) Jun-02 (51.3) Nov-04 (72.4) NS NS NS Reach 09 (RM 296) Jun-02 (629) Nov-04 (432) NS SS SS Reach 11 (RM 293.5) Nov-04 (147) Dec-06 (295) NS NS S Reach 13 (RM 281) Jul-07 (139.6) Jul-08 (82.7) S NS S S NS Dec-06 (296) NS Jul-09 (138.4) S Notes: PFOS = Perfluorooctane sulfonate R01 = Reach 01 RM = River mile Median PFOS Concentrations in ng/g (ppb) provided in parenthesis. N = Not Significant (Mann-Whitney Test determined no significant statistical difference). S = Significant (Mann-Whitney Test determined a significant statistical difference). Table 4-3 Mann-Whitney Test for Significant Differences in Median PFOS Concentrations Channel Catfish Filet Tissue Temporal Evaluation Jul-07 (1.9) Jul-08 (1.1) Jul-09 (1.5) Nov-12 (1.5) Reach 01 Tennessee River (RM 320) Jul-07 (1.9) Jul-08 (1.1) Jul-09 (1.5) S NS NS NS NS NS Reach 02 Point Mallard (RM 307.5) Jun-02 (14.4) Nov-04 (4.36) Dec-06 (4.88) Jun-02 (14.4) Nov-04 (4.36) NS Dec-06 (4.88) NS NS Nov-12 (2.65) NS NS S Nov-04 (404) Dec-06 (747) Jul-07 (8.65) Jul-08 (43.1) Jul-09 (136.4) Nov-12 (283) Reach 05 Bakers Creek (RM 301) Nov-04 (404) Dec-06 (747) Jul-07 (8.65) Jul-08 (43.1) Jul-09 (136.4) NS SS S S NS S S NS NS S S S S NS Jun-02 (2.5) Nov-04 (3.16) Dec-06 (4.29) Nov-12 (4.15) Reach 06 Swan Creek (RM 301.2) Jun-02 (2.5) Nov-04 (3.16) Dec-06 (4.29) S S NS S NS NS Jun-02 (98.9) Nov-04 (11.2) Dec-06 (47.6) Nov-12 (18.6) Reach 09 Fox Creek (RM 296) Jun-02 (98.9) Nov-04 (11.2) Dec-06 (47.6) S SS S NS NS Reach 11 Mallard Creek (RM 293.5) Nov-04 (15.6) Dec-06 (13.4) Nov-04 (15.6) Dec-06 (13.4) NS Nov-12 (15.7) NS NS Reach 13 Tennessee River (RM 281) Jul-07 (7.85) Jul-08 (4.33) Jul-09 (18.4) Jul-07 (7.85) Jul-08 (4.33) S Jul-09 (18.4) S S Nov-12 (10.3) NS S NS Notes: PFOS = Perfluorooctane sulfonate N = Not Significant (Mann-Whitney Test determined no significant statistical difference). R01 = Reach 01 S = Significant (Mann-Whitney Test determined a significant statistical difference). RM = River mile Median PFOS Concentrations in ng/g (ppb) provided in parenthesis. R02 (2.5) R06 (51.3) R09 (629) R02 (114) R05 (3355) R06 (72.4) R09 (432) R11 (147) R02 (19.6) R05 (1127.5) R09 (296) R11 (295) R01 (65.5) R05 (1090.7) R13 (139.6) R01 (59.1) R05 (929.7) R13 (82.7) R01 (48.5) R05 (680.8) R13 (138.4) JUNE 2002 R02 RM 307.5 R02 (2.5) R06 RM 301.2 R06 (51.3) Table 4-4 Mann-W hitney Test for Significant Differences in Median PFOS Concentrations Largemouth Bass Filet Tissue Spatial Evaluation S S NOVEMBER 2004 R02 R05 RM 307.5 RM 301 R02 (114) R05 (3355) R06 RM 301.2 R06 (72.4) R09 RM 296 R09 (432) S S S DECEMBER 2006 R02 R05 RM 307.5 RM 301 R02 (19.6) R05 (1127.5) R09 RM 296 R09 (296) NS S S NS JULY 2007 R01 RM 320 R01 (65.5) R05 RM 301 R05 (1090.7) S S JULY 2008 R01 RM 320 R01 (59.1) R05 RM 301 R05 (929.7) S S JULY 2009 R01 RM 320 R01 (48.5) R05 RM 301 R05 (680.8) S S Table 4-4 (continued) Mann-W hitney Test for Significant Differences in Median Concentrations Largemouth Bass Filet Tissue Spatial Evaluation R01 (32.7) R02 (44.1) R03 (25.7) R04 (159) R05 (435) R06 (50.6) R07 (262) R08 (80.3) R09 (259) R10 (104) R11 (145) R12 (65.8) R13 (71) R14 (75.3) R15 (86.9) R01 RM 320 R01 (32.7) R02 RM 307.5 R02 (44.1) R03 RM 307.5 R03 (25.7) R04 RM 303 R04 (159) R05 RM 301 R05 (435) NOVEMBER 2012 R06 R07 RM 301.2 RM 298 R06 (50.6) R07 (262) R08 RM 298 R08 (80.3) R09 RM 296 R09 (259) R10 RM 294 R10 (104) R11 RM 293.5 R11 (145) R12 RM 285 R12 (65.8) R13 RM 281 R13 (71) R14 RM 273 R14 (75.3) S NS NS S SS S S S NS S NS S S S S S S NS NS S S S S NS S NS S S S S NS NS S NS S S S S NS S S S NS S S S S NS NS S S NS S NS S S S S S NS S NS S S S S S S S S NS S NS S NS S S S S S S S NS S NS S S S NS NS S S S S S NS S NS S NS S NS NS NS Notes: PFOS = Perfluorooctane sulfonate R01 = Reach 01 RM = River mile Median PFOS Concentrations in ng/g (ppb) provided in parentheses. Values in Bold are from comparisons between adjacent reaches. N = Not Significant (Mann-Whitney Test determined no significant statistical difference). S = Significant (Mann-Whitney Test determined a significant statistical difference). Table 4-5 Mann-Whitney Test for Significant Differences in Median PFOS Concentrations Channel Catfish Filet Tissue Spatial Evaluation R02 (14.4) R06 (2.5) R09 (98.9) JUNE 2002 R02 RM 307.5 R02 (14.4) R06 RM 301.2 R06 (2.5) NS SS R02 (4.36) R05 (404) R06 (3.16) R09 (11.2) R11 (15.6) NOVEMBER 2004 R02 R05 R06 RM 307.5 RM 301 RM 301.2 R02 (4.36) R05 (404) R06 (3.16) R09 RM 296 R09 (11.2) S NS S S NS R02 (4.88) R05 (747) R06 (4.29) R09 (47.6) R11 (13.4) DECEMBER 2006 R02 R05 R06 RM 307.5 RM 301 RM 301.2 R02 (4.88) R05 (747) R06 (4.29) R09 RM 296 R09 (47.6) S NS S S S R01 (1.9) R05 (8.65) R13 (7.9) JULY 2007 R01 RM 320 R01 (1.9) R05 RM 301 R05 (8.65) S NS R01 (1.1) R05 (43.1) R13 (4.33) JULY 2008 R01 RM 320 R01 (1.1) R05 RM 301 R05 (43.1) S S R01 (1.49) R05 (136.4) R13 (18.4) JULY 2009 R01 RM 320 R01 (1.49) R05 RM 301 R05 (136.4) S NS R01 (1.5) R02 (2.65) R03 (2.01) R04 (7.85) R05 (283) R06 (4.15) R07 (29.2) R08 (4.89) R09 (18.6) R10 (13.4) R11 (15.7) R12 (10.7) R13 (10.3) R14 (8.01) R15 (7.33) R01 (1.5) R01 RM 320 Table 4-5 (continued) Mann-Whitney Test for Significant Differences in Median PFOS Concentrations Channel Catfish Filet Tissue Spatial Evaluation R02 (2.65) R02 RM 307.5 R03 (2.01) R03 RM 307.5 R04 (7.85) R04 RM 303 R05 (283) R05 RM 301 NOVEMBER 2012 R06 (4.15) R07 (29.2) R08 (4.89) R06 R07 R08 RM 301.2 RM 298 RM 298 R09 (18.6) R09 RM 296 R10 (13.4) R10 RM 294 R11 (15.7) R11 RM 293.5 R12 (10.7) R12 RM 285 R13 (10.3) R13 RM 281 R14 (8.01) R14 RM 273 NS NS NS SSS SSSS NS S NS S S S S S NS S S S S S NS S NS S S S S NS S S NS S S S S NS S S S NS NS S S S NS S S S NS NS NS S S S NS S S S NS S NS S S S S NS S S S NS S NS S NS S S S NS S S S NS S NS S NS NS S S S NS S S S NS S S S NS NS NS Notes: PFOS = Perfluorooctane sulfonate R01 = Reach 01 RM = River mile Median PFOS Concentrations in ng/g (ppb) provided in parentheses. Values in Bold are from comparisons between adjacent reaches. N = Not Significant (Mann-Whitney Test determined no significant statistical difference). S = Significant (Mann-Whitney Test determined a significant statistical difference). Table 4-6 Mann-Kendall Test for Trend in Surface Water PFOS Concentrations Location R M 3 0 7 .5 (Point M allard) R M 301 (B akers C reek mouth) R M 2 9 6 (Fox C reek mouth) Surface Water PFOS Trend D ecreasing D ecreasing D ecreasing M ann-K endall test for trend at significance level of a = 0 .0 5 5. SUMMARY OF KEY FINDINGS The fall 2012 fish and surface water study performed over 56 miles of the Tennessee River and described in this report represents the most comprehensive data set developed to date for PFOS concentrations in the study area. This study was designed to support both the qualitative and quantitative evaluation of current conditions, including the current variation between sampling locations and the identification of trends over time by comparisons with relevant data collected since 2000 in past studies. The following key findings can be drawn from this evaluation. 5.1 TEMPORAL TRENDS IN FISH FILET TISSUE CONCENTRATIONS Examination of time series box plots, bar graphs and median concentrations indicates generally declining levels of PFOS in fish tissue over the period of record. Mann-Kendall testing indicated statistically significant decreasing trends in largemouth bass PFOS concentrations at the RM281 (below Spring Creek), Fox Creek mouth (Reach 09; RM296) and Bakers Creek mouth (Reach 05; RM301) locations. Mann-Whitney testing indicated that reductions in median largemouth bass PFOS concentrations over the period of record were statistically significant for all but the Swan Creek mouth (Reach 06; RM301.2) and Mallard Creek mouth (R eachll; RM293.5) locations. However, the Mallard Creek mouth median largemouth bass PFOS concentration was statistically significantly lower in 2012 than the median from the previous sampling in 2006. The Swan Creek mouth PFOS tissue concentrations have been lower than those along the southern shoreline over the period of record. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 ReportYDraft Final Fall 2012 Fish and Surface Water Report.doc 5-1 Mann-Kendall testing indicated that statistically significant decreasing trends in channel catfish PFOS concentrations occurred at the Bakers Creek mouth (Reach 05; RM301) and Point Mallard (Reach 02; RM307.5) locations. Mann-Whitney testing indicated that reductions in median channel catfish PFOS concentrations over the period of record were statistically significant for the Bakers Creek mouth (Reach 05; RM301) and Fox Creek mouth (Reach 09; RM296) locations. 5.2 SPATIAL EVALUATION OF FISH FILET TISSUE PFOS CONCENTRATIONS For both species of fish evaluated, the highest median PFOS concentrations occur along the southern shoreline in the immediate vicinity of the City of Decatur. Fish tissue data indicate that several sources along this stretch of river contribute to the concentration of PFOS observed. With the exception of Swan Creek (Reach 06; RM301.2) relative to Point Mallard (Reach 02; RM307.5), all median largemouth bass and channel catfish PFOS concentrations at downstream locations were statistically significantly higher than the upstream sampling locations (Reaches 01, 02 and 03; RM320 and RM307.5). The median largemouth bass PFOS concentration at Betty Rye and Dry Branch (Reach 04; RM303) was not statistically significantly different from the median concentrations at Reaches 05, 07, 08, 09, 10 or 11 (RM301, RM298, RM298, RM296, RM294 and RM293.5, respectively). Similarly, the Bakers Creek mouth (Reach 05; RM301) median concentration was not statistically significantly different from the respective Reach 07, 09 or 11 median concentrations. No statistically significant differences were found in largemouth bass or catfish median concentrations among the northern shoreline locations at Swan Creek mouth (Reach 06; RM301.2), Round Island Creek mouth (Reach 08; RM298) and Browns Ferry (Reach 10; RM294). Median largemouth bass and channel catfish J:\FOLDERS.0-9'3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012'Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 5-2 PFOS concentrations at the Betty Rye Creek and Dry Branch mouth (Reach 04; RM303) and Bakers Creek mouth (Reach 05; RM301) southern shoreline locations were statistically significantly higher than medians at the cross river northern shoreline locations. The first instances of statistically significantly lower median PFOS concentrations along the southern shoreline below Decatur were observed for channel catfish at the Fox Creek mouth (Reach 09; RM296) location and for largemouth bass at the Mallard Creek mouth (Reach 11; RM293.5) location. No statistically significant differences in median concentrations were observed between the Mallard Creek mouth and the Elk River mouth (Reach 12; RM285) location on the northern shoreline for largemouth bass or between the Fox Creek mouth and the northern shoreline Round Island Creek (Reach 08; RM298) mouth location for channel catfish. Relative to the Mallard Creek location (Reach 11; RM293.5), channel catfish median PFOS concentrations were statistically significantly lower at the downstream Elk River mouth (Reach 12; RM285) and below Spring Creek (Reach 13; RM281) locations in W heeler Reservoir and the two Wilson Reservoir locations (Reach 14 and 15). No statistically significant differences in largemouth bass and channel catfish median concentrations were observed between the farthest downstream location in Wheeler Reservoir (below Spring Creek; RM281) and the Wilson Reservoir sampling locations. 5.3 EVALUATION OF SURFACE WATER PFOS CONCENTRATIONS Examination of time series plots, bar graphs and median concentrations indicates generally declining levels of PFOS in surface water over the period of record. As noted in previous work, the highest PFOS concentrations are observed along the southern shoreline in the immediate vicinity of Decatur, AL, and generally J:\FOLDERS.0-9'3M-DECATYPhase_3_Work_PlanYFish sampling PFOS 2012\Fall 2012 ReportYDraft Final Fall 2012 Fish and Surface Water Report.doc 5-3 close to shore. Furthermore, the data indicate that several sources along this stretch of river contribute to the concentrations observed. The PFOS results for Transect 1, the farthest upstream transect, do not vary appreciably and are characterized by a median PFOS concentration of 0.00653 ng/mL (ppb). For the area studied, these transect results establish the upstream background levels in surface water. The PFOS results for Transect 9, the farthest downstream transect, also do not vary appreciably with a median PFOS concentration of 0.0203 ng/mL (ppb). Statistically significant decreasing trends in surface water PFOS concentrations were identified at the Fox Creek mouth, Bakers Creek and Point Mallard locations. Surface water PFOS concentrations from cross river transect sampling indicate that the principal sources of PFOS enter the river along the industrialized southern shoreline in the vicinity of Decatur. The highest surface water PFOS concentrations were observed at the sampling locations closest to the southern bank of the river. Slow mixing of PFOS across the river indicates bank attachment of plumes entering along the southern shoreline with complete mixing occurring between Mallard Creek mouth at RM293.5 and below Spring Creek at RM281. An increase in average PFOS concentrations between the farthest downstream transect samples in Wheeler Reservoir below Spring Creek and the transect samples from Wilson Reservoir below Shoal Creek indicates potential PFOS sources entering the Tennessee River in the vicinity of Wheeler Dam. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 5-4 6. REFERENCES ADEM (Alabama Department of Environmental Management). 2012. Fish Tissue Monitoring Program Sample Collection Procedures SOP #2300 Rev. 2.0. Alabama Department of Environmental Management. Montgomery, AL. Entrix, Inc. 2001. SelectedFluorochemicals in the Decatur, Alabama Area. AR226-1109. Entrix, Inc. East Lansing, MI. Entrix, Inc. 2003. Survey o f Selected Fluorochemicals in the Decatur, Alabama Area 2002 Sampling. AR226-1364. Entrix, Inc. East Lansing, MI. EPA (U.S. Environmental Protection Agency). 1993. Fish Field and Laboratory Methods for Evaluating the Biological Integrity o f Surface Waters. EPA. Environmental Monitoring Systems Laboratory. Cincinnati, OH. EPA. 2000. Guidance fo r Assessing Chemical Contaminant Data fo r Use in Fish Advisories, Volume I, Fish Sampling and Analysis. Third Edition. EPA Office of Water. Washington, District of Columbia. EPA. 2012. ProUCL Version 4.1.01. Statistical Software. EPA Office of Research and Development (ORD) Site Characterization and Monitoring Technical Support Center (TSC). http://www.epa.gov/osp/hstl/tsc/software.htm. Hansen, K.J., H.O. Johnson, J.S. Eldridge, J.L. Butenhoff and L.A. Dick. 2002. Quantitative Characterization of Trace Levels of PFOS and PFOA in the Tennessee River. Environmental Science and Technology. (36):1681-1685. Malinsky, M.D., C.B. Jacoby, W.K. Reagen. 2011. Determination of perfluorinated compounds in fish fillet homogenates: method validation and application to fillet homogenates from the Mississippi River. Anal. Chim. Acta. 63: 248-257. NCSS. 2012. NCSS8 Statistical Analysis and Graphical Software. NCSS, LLC. Kaysville, Utah. WESTON (Weston Solutions, Inc.). 2008. Perfluorinated Sulfonates Data Report, 3M Decatur, Alabama Facility, Site-Related Environmental Monitoring Program. Weston Solutions, Inc., W est Chester, PA. September 2008. WESTON. 2012a. Revised Work Plan fo r Fall 2012 Fish and Surface Water Sampling in the Tennessee River. Weston Solutions, Inc., W est Chester, PA. October 2012. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 ReportYDraft Final Fall 2012 Fish and Surface Water Report.doc 6-1 WESTON. 2012b. Fish Sampling and Analysis fo r PFOS in Largemouth Bass from the Tennessee River in the Vicinity o f the 3M Decatur Facility. Weston Solutions, Inc., West Chester, PA. May 2012. Wolf, S.T. and W.K. Reagen. 2011. Method for the determination of perfluorinated compounds (PFCs) in water by solid-phase extraction and liquid chromatography/tandem mass spectrometry (LC/MS/MS). Anal. Methods, 3: 1485-1493. J:\FOLDERS.0-9\3M-DECAT\Phase_3_Work_Plan\Fish sampling PFOS 2012\Fall 2012 Report\Draft Final Fall 2012 Fish and Surface Water Report.doc 6-2 APPENDIX A INTERLABORATORY COMPARISON Fall 2012 Tennessee River Fish and Surface Water Study Interlaboratory Comparison of PFC Results During the fall 2012 fish sampling campaign in the Tennessee River, all primary largemouth bass and channel catfish tissue samples were collected from the right side of the fish specimens, homogenized by TestAmerica and sent to the 3M Environmental Laboratory in St. Paul, Minnesota for analysis. Among other quality assurance/quality control (QA/QC) provisions, a subset of duplicate samples at a 10% duplicate to primary sample ratio were collected from the left side filet, homogenized by TestAmerica and sent to the AXYS Analytical Laboratory in British Columbia, Canada for analysis as split samples. All samples were analyzed at both laboratories for the following perfluorochemicals (PFCs): perfluorooctane sulfonate (PFOS), perfluorobutanoic acid (PFBA), perfluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA), perfluorobutanesulfonate (PFBS), perfluorohexanesulfonate (PFHS), and perfluorooctanesulfonamide (FOSA). Relative percent difference (RPD) values were calculated between the split sample concentrations obtained by the AXYS laboratory of left side filets and the results from the 3M Environmental Laboratory analyses of the corresponding right side filets (see attached table). RPD values were only calculated where both the primary and duplicate samples had concentrations above the detection limit. This comparison showed good correspondence between the two laboratories for all the PFCs. The calculated RPDs ranged from 0.443 to 49.5% for all the PFCs. In particular, the calculated RPDs ranged from 0.462 - 18.5% (averaging 8.75%) for PFOS and from 0.443 to 49.5% (average of 18.9%) for FBSA. PFBS was detected in both the primary and duplicate filet in only one sample and the associated RPD was 8%. The AXYS fish filet tissue split samples PFOS concentrations were generally slightly lower than the respective 3M Environmental Laboratory PFOS concentrations for the primary samples, especially on the channel catfish samples that had generally lower measured levels. Split samples were also collected of surface water samples from the fish sampling reaches and the transect locations at a 10% duplicate to primary sample ratio. Comparisons of PFC concentrations obtained by the AXYS laboratory analysis of the duplicate surface water samples with the results from the 3M Environmental Laboratory corresponding primary sample analysis results indicated good correspondence with RPD values ranging from 0.710 to 49.9% (with averages ranging from 11.4% for PFHS to 22.7% for PFBA) for the surface water (see attached table). FOSA was detected in the primary sample and duplicate in only one surface water sample and the associated RPD was 3.86%. The AXYS surface water PFOS concentrations in the duplicate were generally slightly lower than the respective 3M Environmental Laboratory PFOS concentrations for the primary samples, with an average RPD of 13.9%. In conclusion, the overall comparison of PFC concentrations measured in all fish and surface water samples analyzed by both the laboratories indicated good agreement between the laboratories and reinforce the validity of the results of this study. Interlaboratory Analytical Comparison 3M Environmental Laboratory and AXYS Analytical Services Laboratory Fall 2012 Fish and Surface Water Sampling Tennessee River Sample ID MSF087 MSF088D (MSF087) MSF109 MSF110D (MSF109) MSF092 MSF093D (MSF092) MSF170 MSF171D (MSF170) MSF125 MSF126D (MSF126) MSF006 MSF008D (MSF006) MSF030 MSF032D (MSF030) MSF052 MSF054D (MSF052) MSF062 MSF064D (MSF062) IPF070 IPF072D (IPF070) IPF148 IPF150D (IPF148) IPF135 IPF136D (IPF135) IPF153 IPF154D (IPF153) IPF155 IPF156D (IPF155) IPF018 IPF019D (IPF018) T1-01 T2-02 T3-03 T4-04 T5-05 T6-06 T7-07 T8-08 T9-09 R2 -L3 R05 - L1 R6 - L2 R10 - L3 R15 - L1 Date 11/9/2012 11/9/2012 11/15/2012 11/15/2012 T17157212 11/15/2012 11/29/2012 11/29/2012 11/16/2012 11/16/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/9/2012 11/9/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 11/16/2012 11/16/2012 11/19/2012 11/19/2012 11/20/2012 11/20/2012 11/19/2012 11/19/2012 11/17/2012 11/17/2012 Analytical Laboratory 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS Sampling Reach No. R02 R02 R03 R03 R04 R04 R05 R05 R06 R06 R09 R09 R10 R10 R14 R14 R15 R15 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS R01 R01 R07 R07 R08 R08 R10 R10 R11 R11 R12 R12 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS _ _ _ _ _ _ _ _ _ 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS 3M Environmental AXYS R02 R02 R05 R05 R06 R06 R10 R10 R15 R15 Location Point Mallard Point Mallard RM 307.5 (Backwater) RM 307.5 (Backwater) Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Bakers Creek Bakers Creek Swan Creek Swan Creek Fox Creek Fox Creek Browns Ferry Browns Ferry Big Nance Creek Big Nance Creek Shoal Creek Shoal Creek Average RPD: RM 320 RM 320 River Mile 298 River Mile 298 Round Island Creek Round Island Creek Browns Ferry Browns Ferry Mallard Creek Mallard Creek Elk River Elk River Average RPD: Transect 1 (RM 320) Transect 1 (rM 320) Transect 2 (RM 307.5) Transect 2 (rM 307.5) Transect 3 (RM 303) Transect 3 (RM 303) Transect 4 (rM 30l) Transect 4 (rM 30l) Transect 5 (rM 298) Transect 5 (rM 298) Transect 6 (RM 296) Transect 6 (RM 296) Transect 7 (RM 293.5) Transect 7 (rM 293.5) Transect 8 (RM 281) Transect 8 (RM 281) Transect 9 (RM 264) Transect 9 (RM 264) Average RPD: Point Mallard Point Mallard Bakers Creek Bakers Creek Swan Creek Swan Creek Browns Ferry Browns Ferry Shoal Creek Shoal Creek Average RPD: PFBA PFHxA PFHpA Result <0.250 <0.488 <1.00 <0.495 <0.250 <0.474 <0.250 <0.446 <1.00 <0.481 <0.250 <0.495 <0.250 <0.469 <0.100 <0.495 <0.100 <0.398 --- <0.250 <0.490 <0.250 <0.452 <0.250 <0.481 <0.250 <0.503 <0.250 <0.472 <0.250 <0.483 -- <5.00 5.42 6.72 8.61 <5.00 5.91 <5.00 6.09 <5.00 6.14 <5.00 6.01 8.42 9.21 6.28 8.89 8.66 8.91 15.4 6.13 8.82 58.9 75.5 <5.00 6.18 <5.00 6.79 7.92 10.4 29.3 RPD Result RPD Result Largemouth Bass Fish Tissue ng/g; ppb) NC <0.101 NC <0.503 <0.488 <0.488 NC <2.52 <0.495 NC <0.252 <0.495 <0.101 <0.503 NC <0.474 NC <0.474 NC <0.252 <0.446 NC <0.252 <0.446 <2.52 <0.252 NC <0.481 NC <0.481 NC <0.0503 <0.495 NC <0.252 <0.495 <0.101 0.198 [a] NC <0.469 NC <0.469 <0.101 <0.101 NC <0.495 NC <0.495 NC <0.101 <0.398 NC <0.101 <0.398 --- --- Channel Catfish Fish Tissue (ng/g; ppb) NC <0.0503 <0.490 NC <0.101 <0.490 NC <0.101 <0.452 NC <0.252 <0.452 NC <0.101 <0.481 NC <0.252 <0.481 NC <0.252 <0.503 NC <0.252 <0.503 NC <0.252 <0.472 NC <0.252 <0.472 NC <0.0503 <0.483 NC <0.252 <0.483 --- --- Surface Water - Surface Water Transects (ng/L; ppt) NC <2.00 1.69 NC <2.00 1.16 24.7 5.16 4.93 4.56 4.80 6.01 NC <2.00 1.67 NC <2.00 1.15 NC <2.00 1.58 NC <2.00 1.28 NC <2.00 1.56 NC <2.00 1.29 NC 2.31 2.09 10.0 <2.00 2.11 B 8.96 4.05 4.65 13.8 3.24 3.93 34.4 4.51 3.61 22.2 <2.00 3.11 7.12 3.37 2.85 5.42 27.1 3.73 18.3 14.7 Surface Water - Fish Reaches (ng/L; ppt) 36.0 4.83 3.83 23.1 4.70 4.64 24.7 72.5 66.1 9.24 56.8 64.7 NC <2.00 1.62 NC <2.00 <1.21 NC 2.73 1.64 49.9 <2.00 1.24 27.1 4.24 4.21 0.710 2.34 3.88 20.7 21.3 RPD NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC 22.4 NC NC NC NC 19.2 NC 10.1 1.28 13.0 NC NC 49.5 PFOA Result RPD <0.240 <0.488 <0.480 <0.495 <0.240 <0.474 0.643 <0.446 <0.480 <0.481 <0.480 <0.495 <0.480 <0.469 <0.240 <0.495 <0.240 <0.398 --- NC NC NC NC NC NC NC NC NC <0.240 <0.490 <0.240 <0.452 <0.240 <0.481 <0.480 <0.503 <0.480 <0.472 <0.480 <0.483 --- NC NC NC NC NC NC <1.92 2.54 12.6 10.5 2.78 3.07 2.25 2.53 2.52 2.65 4.38 3.62 12.5 9.73 7.49 7.56 11.6 9.6 13.7 NC 18.2 9.91 11.7 5.03 19.0 24.9 0.930 18.9 9.28 9.14 246 210 <4.80 2.75 3.52 2.56 11.5 10.6 14.3 1.52 15.8 NC 31.6 8.14 Minimum RPD Fish Maximum RPD: Average RPD: Minimum RPD Surface Water Maximum RPD: Average RPD: Notes: 3M Environmental fish tissue concentrations are the average of the primary and duplicate analytical results in ng/g (ppb) on a wet weight basis 3M Environmental surface water concentrations are the average of primary and duplicate analytical results in ng/L (ppt) AXYS fish tissue concentrations are results from analysis of a single sample aliquot in ng/g (ppb) AXYS surface water concentrations are results from analysis of a single sample aliquot in ng/L (ppt) Summary Statistics --- --- --- ----- --- --- ----- --- --- --2.85 0.710 1.28 0.930 36.0 49.9 49.5 31.6 22.7 17.8 19.3 13.8 AXYS analytical suite did not include FBSA, N-MeFOSAA or N-EtFOSA. NC - Not Calculated. One or more data points was below the detection limit. B - PFHpA detected in laboratory blank at 1.48 ng/L. Analyte found in sample and associated blank. PFBS Result RPD <0.252 <0.976 <0.252 <0.990 <0.252 <0.948 2.21 2.04 <0.252 <0.962 <1.10 <0.99 0.590 <0.939 <0.252 <0.990 <0.252 <0.797 8.00 NC NC NC 8.00 NC NC NC NC NC <0.252 <0.980 <0.252 <0.905 <0.252 <0.962 <0.503 < 1.01 <0.503 <0.943 <1.10 <0.966 --- NC NC NC NC NC NC <5.00 <2.01 <5.00 3.83 <5.00 <2.07 <5.00 <2.37 <5.00 <2.23 <5.00 2.14 19.0 15.0 11.2 11.4 12.8 12.6 8.96 NC NC NC NC NC NC 23.5 1.77 1.57 <5.00 2.70 124 166 <5.00 2.69 <5.00 <2.15 14.8 20.9 31.6 NC 29.0 NC NC 34.2 PFHS Result RPD <0.250 <0.976 <0.100 <0.990 <0.250 <0.948 0.783 <0.893 <0.100 <0.962 <0.250 <0.990 <0.250 <0.939 <0.250 <0.990 <0.250 <0.797 --- NC NC NC NC NC NC NC NC NC <0.250 <0.980 <0.250 <0.905 <0.250 <0.962 <0.250 < 1.01 <0.250 <0.943 <0.250 <0.966 --- NC NC NC NC NC NC <5.00 <2.01 <5.00 <2.03 <5.00 <2.07 <5.00 <2.37 <5.00 <2.23 <5.00 <2.01 <5.00 <2.18 <5.00 <2.10 <5.00 <2.32 -- NC NC NC NC NC NC NC NC NC <5.00 <1.9 39.8 35.5 <5.00 <2.42 <5.00 <2.15 <5.00 <2.2 11.4 NC 11.4 NC NC NC PFOS Result RPD 49.0 56.6 21.8 22.0 434 432 373 345 40.8 [c] 43.9 173 186 65.2 56.1 93.5 [b] 91.7 103 99.8 6.47 14.4 0.913 0.462 7.80 7.32 7.24 15.0 1.94 3.16 1.81 1.66 69.6 57.8 4.89 [b] 4.12 16.3 14.5 11.9 12.3 11.7 10.2 12.2 8.65 18.5 17.1 11.7 3.31 13.7 6.13 5.26 15.3 9.93 8.44 16.2 6.94 6.68 3.82 7.29 6.30 14.6 7.20 5.10 34.1 9.72 7.45 26.4 20.2 17.3 15.5 13.3 12.8 3.83 19.2 17.7 8.13 17.6 8.94 7.59 16.3 385 388 0.776 6.72 7.66 13.1 6.49 5.48 16.9 19.9 22.0 10.0 11.4 FOSA Result RPD 0.379 <0.585 <0.101 <0.594 4.69 4.07 59.9 55.4 0.200 [c] <0.577 2.26 2.25 0.571 <0.563 2.11 1.52 2.42 1.46 --- NC NC 14.2 7.81 NC 0.443 NC 32.5 49.5 0.153 [b] <0.588 3.64 3.87 <0.253 <0.577 0.816 <0.603 1.83 1.74 1.21 0.846 15.5 NC 6.13 NC NC 5.04 35.4 <2.00 <1.01 <2.00 <1.02 <2.00 <1.04 <2.00 <1.18 <2.00 <1.11 <2.00 <1.00 <2.00 <1.09 <2.00 <1.05 <2.00 <1.16 --- NC NC NC NC NC NC NC NC NC <2.00 <0.952 21.1 20.3 <2.00 <1.21 <2.00 <1.08 <2.00 <1.10 3.86 NC 3.86 NC NC NC 8.00 --- 0.462 0.443 8.00 --- 18.5 49.5 8.00 --- 8.75 18.9 1.57 11.4 0.776 3.86 34.2 11.4 34.1 3.86 18.0 11.4 13.9 3.86 [a] - Value reported for single replicate results, duplicate result was <LLOQ [b] - RPD > 20% [c] - Value reported for single result, duplicate sample analysis had peak shift out of retention time window and no result determined Lance R . L eF leur D irector Alabama Department of Environmental Management adem.alabama.gov 1400 Coliseum Blvd. 36110-2400 Post Office Box 301463 Montgomery, Alabama 36130-1463 (334)271-7700 FAX (334) 271-7950 R obert J. B entley G overnor May 13,2013 Gary A. Hohenstein, Manager Environmental and Regulatory Affairs 3M EHS Operations 3M Center, Bldg 224-5W-03 St. Paul, MN 55144-1000 Dear Mr. Hohenstein: We appreciate 3M agreeing to work with the Department regarding the fall 2012 PFC Tennessee River sampling project. This data is greatly needed and will help everyone make sound decisions regarding future actions along the river. The Department has conducted a review of the data from the split samples collected related to the primary samples. Based on this review, we believe that both the surface water and fish samples are in good agreement. This agreement between the samples reinforces the validity of the results of this study. If 1can be of any help, please don't hesitate to give me a call at (334) 394-4382. Sincerely, Steven O. Jenkins, Chief Field Operations Division Birm ingham Branch 110 Vulcan Road Birmingham, AL 35209-4702 (205) 942-6168 (205) 941-1603 (FAX) Decatur Branch 2715 Sandlin Road, S. W. Decatur, AL 35603-1333 (256) 353-1713 (256) 340-9359 (FAX) M obile Branch 2204 Perimeter Road Mobile, AL 36615-1131 (251) 450-3400 (251) 479-2593 (FAX) M obile-Coastal 4171 Commanders Drive Mobile, AL 36615-1421 (251) 432-6533 (251) 432-6598 (FAX) APPENDIX B SUMMARY OF PERFLUOROCHEMICAL ANALYTICAL RESULTS Sam ple ID MSF071 MSF073D (MSF071) MSF074 MSF075 MSF080 MSF081 MSF084 MSF085 MSF086 MSF087 MSF088D (MSF087) MSF089 MSF109 MSF110D (MSF109) MSF111 MSF115 MSF119 MSF121D (MSF119) MSF124 MSF090 MSF091 MSF092 MSF093D (MSF092) MSF099 MSF102 MSF169 MSF170 MSF171D (MSF170) MSF172 MSF173 MSF174 MSF094 MSF096 MSF125 MSF126D (MSF126) MSF167 MSF168 MSF127 MSF128 MSF129 MSF130 MSF132D (MSF130) MSF131 MSF141 MSF143D (MSF141) MSF142 MSF144 MSF145 MSF146 MSF001 MSF004 MSF005D (MSF004) MSF006 MSF008D (MSF006) MSF139 MSF140 Date 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/15/2012 11/15/2012 11/16/2012 11/16/2012 11/29/2012 11/29/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/21/2012 11/21/2012 Analytical Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental S am p lin g Reach No. R01 R01 R01 R01 R01 R01 R02 R02 R02 R02 R02 R02 R03 R03 R03 R03 R03 R03 R03 R04 R04 R04 R04 R04 R04 R05 R05 R05 R05 R05 R05 R06 R06 R06 R06 R06 R06 R07 R07 R07 R07 R07 R07 R08 R08 R08 R08 R08 R08 R09 R09 R09 R09 R09 R09 R09 Appendix B Table 1 Largemouth Bass Fish Morphometries and PFC Concentrations Sampling Reach RM 320 RM 320 RM 320 RM 320 RM 320 RM 320 Point Mallard Point Mallard Point Mallard Point Mallard Point Mallard Point Mallard RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Bakers Creek Bakers Creek Bakers Creek Bakers Creek Bakers Creek Bakers Creek Swan Creek Swan Creek Swan Creek Swan Creek Swan Creek Swan Creek River Mile 281 River Mile 281 River Mile 281 River Mile 281 River Mile 281 River Mile 281 Round Island Creek Round Island Creek Round Island Creek Round Island Creek Round Island Creek Round Island Creek Fox Creek Fox Creek Fox Creek Fox Creek Fox Creek Fox Creek Fox Creek River Mile RM320 RM320 RM320 RM320 RM320 RM320 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM303 RM303 RM303 RM303 RM303 RM303 RM301 RM301 RM301 RM301 RM301 RM301 RM301.2 RM301.2 RM301.2 RM301.2 RM301.2 RM301.2 RM281 RM281 RM281 RM281 RM281 RM281 RM298 RM298 RM298 RM298 RM298 RM298 RM296 RM296 RM296 RM296 RM296 RM296 RM296 Length (inches) 13 3/8 13 3/8 16 1/4 13 1/8 13 1/8 14 1/8 16 1/2 18 15 5/8 17 1/8 17 1/8 13 5/8 15 1/4 15 1/4 16 1/8 15 7/8 16 1/8 16 1/8 17 1/2 17 1/2 14 1/2 17 3/4 17 3/4 13 1/2 16 1/8 16 7/8 16 3/4 16 3/4 17 3/4 15 3/8 17 1/4 17 3/8 17 3/8 17 3/8 17 3/8 15 3/8 15 7/8 15 14 7/8 13 1/4 13 1/4 13 1/4 13 1/4 15 1/4 15 1/4 14 5/8 15 3/8 15 3/8 17 1/2 17 3/8 14 1/2 14 1/2 12 3/8 12 3/8 17 1/2 16 3/8 Weight (pounds) 1.3 1.3 2.5 1.1 1.0 1.2 2.6 3.0 2.1 2.4 2.4 1.1 1.9 1.9 2.5 2.2 2.7 2.7 3.1 3.4 1.8 2.9 2.9 1.4 2.7 2.4 2.5 2.5 3.1 2.2 3.1 2.9 3.0 2.5 2.5 2.3 2.4 2.1 1.8 1.2 1.3 1.3 1.2 2.1 2.1 1.7 1.8 2.0 3.2 1.6 1.1 1.1 0.7 0.7 2.8 2.6 PFBA <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.488 <0.250 <1.00 <0.495 <1.00 <1.00 <1.00 <1.00 <1.00 <0.250 <0.250 <0.250 <0.474 <0.250 <0.250 <0.250 <0.250 <0.446 <0.250 <0.250 <0.250 0.860 [a] <0.250 <1.00 <0.481 <0.250 <0.250 <1.00 <1.00 <1.00 <1.00 <0.250 <0.250 [c] <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.495 <0.250 <0.250 PFHxA <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.101 <0.488 0.260 [a] <2.52 <0.495 <2.52 <2.52 <2.52 <2.52 <2.52 0.151 [a] 0.174 [a] <0.101 <0.474 <0.101 <0.101 <0.252 <0.252 <0.446 <0.252 <0.252 0.294 [a] 0.132 0.144 <2.52 <0.481 <0.252 <0.252 <2.52 <2.52 <2.52 <2.52 0.0992 [a] <0.101 0.136 <0.101 <0.101 0.122 [a] <0.101 <0.101 <0.0503 <0.0503 <0.0503 <0.0503 <0.495 0.0942 [a] <0.101 PFHpA <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0.110 [a] <0.101 <0.503 <0.488 <0.503 <0.252 <0.495 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.503 <0.474 <0.503 <0.503 <0.252 <0.252 <0.446 <0.252 <0.252 <0.252 <0.503 <0.503 <0.252 <0.481 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.495 <0.252 <0.252 PFOA <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.488 <0.240 <0.480 <0.495 <0.480 <0.480 <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.474 <0.240 <0.240 <0.480 0.643 <0.446 <0.480 <0.480 <0.240 <0.240 <0.240 <0.480 <0.481 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 <0.480 <0.480 <0.480 <0.495 <0.240 <0.240 P FC Concentrations (ng/g; ppb) PFBS <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.976 <0.252 <0.252 <0.99 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.948 <0.252 <0.252 <0.503 2.21 2.04 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.962 <0.503 <0.503 0.444 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <1.10 <1.10 <1.10 <1.10 <0.99 <0.252 <0.252 PFHS <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.976 <0.250 <0.100 <0.99 <0.100 <0.100 <0.100 <0.100 <0.100 <0.250 <0.250 <0.250 <0.948 <0.250 <0.250 <0.250 0.783 <0.893 0.391 <0.250 <0.500 <0.250 <0.250 <0.100 <0.962 <0.250 <0.250 0.416 <0.100 0.123 <0.100 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.99 <0.250 <0.250 PFOS 35.8 36.3 32.7 27.9 18.6 37.8 44.1 32.6 49.5 49.0 56.6 40.8 21.8 22 25.7 31.2 23.9 19.9 73.2 104 159 434 432 124 218 435 373 345 96.4 599 521 69.0 46.9 40.8 [d] 43.9 50.6 120 408 262 250 333 324 178 134 143 45.0 76.9 175 80.3 259 167 168 173 186 279 281 FBSA 0.555 0.529 1.72 0.648 0.361 0.802 7.01 2.72 6.87 25.8 ___ 14.8 1.03 ___ 1.57 4.28 2.26 2.06 6.47 28.4 20.4 8.55 ___ 6.10 22.4 67.2 [b] 567 ___ 45.8 135 95.0 28.1 2.95 6.23 ___ 12.1 20.2 182 110 137 119 143 [c] 128 [c] 114 [c] 111 [c] 15.4 [c] 11.4 [c] 15.2 [c] 17.7 [c] 86.2 [b,c] 58.2 [c] 60.9 [c] 79.5 [c] ___ 72.9 [c] 133 [c] FOSA 0.137 0.161 0.215 0.122 0.148 0.135 [a] 1.44 0.580 0.127 [a] 0.379 <0.585 1.22 [b] <0.101 <0.594 0.235 [b] <0.101 0.162 0.141 0.258 [b] 1.71 2.24 4.69 4.07 1.60 1.37 [b] 7.44 59.9 55.4 2.92 9.75 5.20 0.672 0.396 0.200 [d] <0.577 1.28 4.22 4.19 4.00 2.46 8.18 7.21 2.12 1.75 1.83 0.441 [b] 0.243 [a] 0.348 0.465 2.37 3.05 3.08 2.26 2.25 1.73 3.75 N-MeFOSAA <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 0.398 0.122 [b] <0.100 0.284 ___ 0.288 [b] <0.100 ___ <0.100 <0.100 <0.100 <0.100 <0.100 0.289 0.478 0.688 [b] ___ 0.648 1.39 3.55 22.5 ___ 0.545 4.67 1.62 0.446 0.171 <0.100 [d] ___ <0.500 1.21 2.22 1.18 1.65 5.07 5.54 1.69 1.06 1.02 <0.250 <0.250 1.16 0.336 1.45 1.37 1.35 0.962 ___ 1.43 1.85 N-EtFOSAA <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 0.530 [b] <0.250 <0.250 0.318 ___ 0.322 [a] <0.500 ___ <0.500 <0.500 <0.500 <0.500 <0.500 0.350 [a] 0.464 0.704 ___ 0.478 [b] 1.27 3.01 19.0 ___ 0.639 3.46 1.43 <0.250 <0.250 <0.500 [d] ___ <0.500 1.56 1.73 1.08 1.19 2.42 2.87 1.43 0.703 0.753 <0.250 <0.250 0.896 <0.250 1.19 1.17 1.13 0.943 ___ 1.45 1.76 Sam ple ID MSF030 MSF032D (MSF030) MSF033 MSF035 MSF037 MSF038D (MSF037) MSF040 MSF042 MSF043D (MSF042) MSF044 MSF045 MSF157 MSF158 MSF020 MSF021 MSF023 MSF025 MSF027 MSF028D (MSF027) MSF010 MSF011 MSF013 MSF015 MSF017 MSF046 MSF048 MSF050D (MSF048) MSF052 MSF054D (MSF052) MSF054D MSF056 MSF058 MSF060 MSF061 MSF062 MSF064D (MSF062) MSF065 MSF067 PRE MID POST DAL-MSF-LS-10001 DAL-MSF-LS-10003 DAL-MSF-LS-10006 Date 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 ------- 11/3/2012 11/3/2012 11/29/2012 Analytical Laboratory 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental S am p lin g Reach No. R10 R10 R10 R10 R10 R10 R10 R11 R11 R11 R11 R11 R11 R12 R12 R12 R12 R12 R12 R13 R13 R13 R13 R13 R14 R14 R14 R14 R14 R14 R14 R14 R15 R15 R15 R15 R15 R15 Control Control Control Control Control Control Sampling Reach Browns Ferry Browns Ferry Browns Ferry Browns Ferry Browns Ferry Browns Ferry Browns Ferry Mallard Creek Mallard Creek Mallard Creek Mallard Creek Mallard Creek Mallard Creek Elk River Elk River Elk River Elk River Elk River Elk River River Mile 281 River Mile 281 River Mile 281 River Mile 281 River Mile 281 Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek na na na na na na Appendix B Table 1 Largemouth Bass Fish Morphometries and PFC Concentrations River Mile RM294 RM294 RM294 RM294 RM294 RM294 RM294 RM293.5 RM293.5 RM293.5 RM293.5 RM293.5 RM293.5 RM285 RM285 RM285 RM285 RM285 RM285 RM281 RM281 RM281 RM281 RM281 RM273 RM273 RM273 RM273 RM273 RM273 RM273 RM273 RM264 RM264 RM264 RM264 RM264 RM264 na na na na na na Length (inches) 13 13 17 13 13 3/8 13 3/8 13 1/8 15 5/8 15 5/8 14 1/8 15 14 16 1/2 13 5/8 15 3/8 14 5/8 15 13 3/8 13 3/8 13 5/8 16 7/8 13 5/8 13 13 1/4 13 3/8 13 3/8 13 3/8 14 14 14 15 1/8 17 1/8 16 3/8 14 17 3/8 17 3/8 17 1/8 15 7/8 ------- 17 1/4 16 3/8 16 Weight (pounds) 1.1 1.1 2.8 1.1 1.3 1.3 1.1 1.8 1.8 1.6 2.2 1.4 3.0 1.3 2.2 1.8 1.8 1.5 1.5 1.4 3.0 1.4 1.3 1.2 1.4 1.5 1.5 1.5 1.5 1.5 2.1 2.7 2.7 1.7 3.1 3.1 2.8 2.3 ------- ------- P F C Concentrations (ng/g; ppb) PFBA <0.250 <0.469 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 NR <0.100 <0.100 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.100 <0.100 <0.100 <0.100 <0.495 <0.450 <0.100 <0.100 <0.100 <0.100 <0.100 <0.398 <0.100 <0.250 1.42 1.42 1.42 <0.250 <0.250 <0.250 PFHxA <0.101 <0.469 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.252 0.317 fai <0.0503 <0.0503 <0.101 <0.101 <0.101 <0.101 <0.0503 <0.0503 <0.0503 <0.0503 <0.0503 <0.101 <0.101 <0.101 <0.101 <0.495 <0.450 <0.101 <0.101 <0.101 <0.101 <0.101 <0.398 <0.101 <0.0503 <0.101 0.126 [a] 0.170 [a] <0.252 <0.252 <0.252 PFHpA 0.198 [a] <0.469 <0.101 <0.101 <0.101 <0.101 <0.101 0.120 [a] <0.101 0.118 [a] <0.101 <0.252 <0.252 <0.252 <0.252 <0.101 <0.101 <0.101 <0.101 <0.252 <0.252 <0.252 <0.252 <0.252 <0.101 <0.101 <0.101 <0.101 <0.495 <0.450 <0.101 <0.101 <0.101 <0.101 <0.101 <0.398 <0.101 0.176 [a] <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 PFOA <0.480 <0.469 <0.480 <0.480 <0.480 <0.480 <0.480 1.57 [a] NR <0.240 <0.240 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 0.576 [a,c] <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.495 <0.450 <0.240 <0.240 <0.240 <0.240 <0.240 <0.398 <0.240 <0.240 <0.960 <0.240 <0.240 <0.240 <0.240 <0.240 PFBS 0.590 <0.939 0.459 0.219 0.582 [b] 0.996 [b] 0.259 0.131 [b] 0.138 [b] <0.252 <0.252 <0.503 <0.503 <1.10 <1.10 0.184 [b] 0.294 [a] 0.176 [b] 0.859 <1.10 NR <1.10 <1.10 <1.10 <0.252 0.269 [a] <0.252 <0.252 <0.99 <0.901 <0.252 <0.252 <0.252 0.289 <0.252 <0.797 <0.252 <0.252 <0.101 <0.252 <0.252 <0.252 <0.252 <0.252 PFHS <0.250 <0.939 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.99 <0.901 <0.250 <0.250 <0.250 <0.250 <0.250 <0.797 <0.250 <0.250 <0.0960 <0.250 <0.250 <0.500 <0.500 <0.500 PFOS 65.2 56.1 190 207 101.9 106 104 210 [b] 224 116 225 115 145 65.8 98.2 54.9 66.4 65.3 65.9 71.0 98.5 68.9 67.1 79.6 54.1 88.9 81.6 93.5 [b] 91.7 103 54.8 75.3 64.7 75.5 103 99.8 86.9 94.6 0.731 0.523 [a <0.184 [a] 3.95 3.67 4.31 FBSA 22.1 [c] --- 31.5 [c] 41.3 [c] 63.6 [c] 66.4 [c] 35.6 [d 52.8 [c] 55.5 [c] 107 37.9 74.7 67.3 56.7 [c] 23.3 [c] 33.0 [c] 47.9 [b,c] 48.1 [c] 45.0 [d 68.6 [c] 81.7 [c] 84.1 [c] 56.2 [c] 51.3 [d 56.5 129 116 71.1 --- --- 19.3 69.7 74.2 85.9 92.6 --- 46.8 38.6 <0.250 [d] <0.250 <0.250 [c] <0.250 <0.250 <0.250 FOSA 0.571 <0.563 1.12 1.28 0.716 0.750 1.26 1.25 1.32 3.09 1.99 1.20 2.28 1.11 0.699 0.800 0.887 0.988 0.970 1.42 2.03 1.37 1.24 1.09 1.11 2.40 2.11 2.11 1.52 1.69 0.530 1.59 2.48 2.21 2.42 1.46 1.19 1.98 <0.253 <0.253 <0.253 <0.253 <0.253 <0.253 N-MeFOSAA <0.500 --- 1.67 0.596 <0.500 0.523 0.798 2.61 [b] 2.86 1.06 0.768 [b] 0.786 0.974 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 0.546 [a] 0.684 [a] <0.500 0.435 1.00 0.883 0.448 [b] --- --- <0.250 0.404 0.365 [b] 0.393 0.419 --- 0.331 0.313 <0.500 <0.100 <0.250 <0.500 <0.500 <0.500 N-EtFOSAA 0.388 [b] --- 0.845 0.602 0.425 0.464 0.652 1.52 1.62 0.791 0.777 0.835 0.847 <0.500 0.520 [a] 0.326 0.282 0.344 [b] 0.337 <0.500 <0.500 <0.500 <0.500 <0.500 0.315 0.795 0.731 0.458 --- --- 0.226 [b] 0.384 0.447 0.544 0.457 --- 0.284 0.493 <0.500 <0.250 <0.250 <0.500 <0.500 <0.500 Notes: PFC concentrations are the average of the primary and duplicate analytical results expressed in ng/g (ppb) on a wet weight basis. Sample IDs with a "D" suffix are field duplicates obtained from left side filet samples. Field duplicate are samples identified with "D" suffix and the corresponding primary sample ID in parentheses. na - not applicable -- not supplied NR - Sample had LMS prepared and recovery was outside of 100+ 50% and result not reported. * - quantified from a linear-PFOS (L-PFOS) calibration for total-PFOS (sum of linear and branched isomer peaks) in samples, LMSs and LCSs. [a] Value reported for single replicate results, duplicate result was <LOQ. [b] RPD > 20% [c] Sample had LMS prepared and LMS recovery result was outside of 100 + 30%, but within 100 + 50% and was reported with an expanded uncertainty of 50%. [d] Value reported for single result, duplicate sample analysis had peak shift out of retention time window and no result determined. PRE - TN_11-0194-1_1 Sample 1_Pre-Study_Homogenization_Process control MID -TN_11-0194-1_1Sample 2_Mid-Study_Homogenization_Process control POST - TN_11-0194-1_1 Sample 3_Post-Study_Homogenization DAL-MSF-LS-10001 = Pre-Sample Filet; Lab Tag No. TN12-0364-1/13 Bass (250904) DAL-MSF-LS-10003 = Lab Tag No. TN12-0364-3/13-Bass (250906) DAL-MSF-LS-10006 = Post-Sample Filet; Lab Tag No. TN12-0364-2/13 Bass (250905) Post-analysis inspection of samples DAL-MSF-LS-10001 and DAL-IPF-LS-10002 indicated the samples had been switched. Table reflects correction. PFOS = Perfluorooctane sulfonate PFBA = Perfluorobutanoic acid PFHxA = Perfluorohexanoic acid PFHpA = Perfluoroheptanoic acid PFOA = Perfluorooctanoic acid PFBS = Perfluorobutane sulfonate PFHS = Perfluorohexane sulfonate FBSA = Perfluorobutanesulfonamide FOSA =Perfluorooctanesulfonamide N-MeFOSAA = 2-(N-ethyl-PFOSA) acetic acid N-EtFOSAA = 2-(N-methyl-PFOSA) acetic acid Sample ID IPF070 IPF072D (IPF070) IPF076 IPF077 IPF078 IPF079 IPF082 IPF083 IPF112 IPF113 IPF114D (IPF113) IPF116 IPF117 IPF118 IPF120 IPF122 IPF123 IPF100 IPF101 IPF103D (IPF101) IPF106 IPF107 IPF108 IPF161 IPF162D (IPF161) IPF163 IPF164 IPF165 IPF166 IPF095 IPF097 IPF098 IPF104 IPF105 IPF147 IPF148 IPF150D (IPF148) IPF149 IPF151 IPF152 IPF133 IPF134 IPF135 IPF136D (IPF135) IPF137 IPF138 IPF012 IPF014 IPF016D (IPF014) IPF026 IPF029 IPF031 Date 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/9/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/15/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 Analytical Laboratory 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental Sampling Reach No. R01 R01 R01 R01 R01 R01 R02 R02 R02 R02 R02 R02 R03 R03 R03 R03 R03 R04 R04 R04 R04 R04 R04 R05 R05 R05 R05 R05 R05 R06 R06 R06 R06 R06 R07 R07 R07 R07 R07 R07 R08 R08 R08 R08 R08 R08 R09 R09 R09 R09 R09 R09 Sampling Reach RM 320 RM 320 RM 320 RM 320 RM 320 RM 320 Point Mallard Point Mallard Point Mallard Point Mallard Point Mallard Point Mallard RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) RM 307.5 (Backwater) Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Betty Rye & Dry Branch Creek Bakers Creek Bakers Creek Bakers Creek Bakers Creek Bakers Creek Bakers Creek Swan Creek Swan Creek Swan Creek Swan Creek Swan Creek River Mile 298 River Mile 298 River Mile 298 River Mile 298 River Mile 298 River Mile 298 Round Island Creek Round Island Creek Round Island Creek Round Island Creek Round Island Creek Round Island Creek Fox Creek Fox Creek Fox Creek Fox Creek Fox Creek Fox Creek Appendix B Table 2 Channel Catfish Fish Morphometries and PFC Concentrations River Mile RM320 RM320 RM320 RM320 RM320 RM320 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM307.5 RM303 RM303 RM303 RM303 RM303 RM303 RM301 RM301 RM301 RM301 RM301 RM301 RM301.2 RM301.2 RM301.2 RM301.2 RM301.2 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM298 RM296 RM296 RM296 RM296 RM296 RM296 Length (inches) 18 1/4 18 1/4 17 18 7/8 23 7/8 19 3/4 22 1/8 19 1/2 19 3/8 18 1/4 18 1/4 20 20 7/8 19 3/8 20 7/8 21 3/4 15 7/8 16 5/8 21 3/8 21 3/8 19 1/2 15 1/2 15 1/2 18 1/4 18 1/4 19 1/4 21 1/8 18 5/8 22 5/8 20 5/8 21 1/2 18 5/8 18 1/8 14 7/8 19 3/8 22 1/2 22 1/2 16 1/8 18 5/8 20 3/8 23 3/4 16 3/4 18 1/8 18 1/8 15 3/4 15 5/8 17 3/8 16 1/8 16 1/8 18 1/2 18 1/4 18 1/4 Weight (pounds) 1.5 1.5 1.6 2.5 4.3 2.6 3.6 2.1 2.6 2.0 2.0 2.4 2.8 1.7 2.0 3.6 1.2 1.8 2.8 2.8 2.5 1.0 0.9 2.0 2.0 2.8 3.6 2.3 3.1 2.7 3.4 1.9 1.8 0.9 2.3 3.2 3.2 1.3 1.7 2.6 3.9 1.3 1.6 1.6 1.0 1.2 1.8 1.2 1.2 2.0 2.2 2.1 PFBA <0.250 <0.490 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <1.00 <1.00 <1.00 <1.00 <1.00 <1.00 <1.00 <1.00 <1.00 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.452 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.481 <0.250 <0.250 <0.250 <0.250 <0.250 2.34 [a] <0.250 <0.250 PFHxA <0.0503 <0.490 0.139 [a] <0.0503 <0.0503 <0.0503 <0.0503 0.137 [a] <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 0.0965 [a] 0.137 [a] <0.101 <0.101 <0.101 <0.101 <0.101 <0.452 <0.101 <0.252 <0.252 0.417 [a] <0.101 <0.101 <0.481 <0.101 <0.101 <0.0503 <0.0503 <0.0503 1.78 [a] <0.101 <0.101 PFC Concentrations (ng/g; ppb) PFHpA <0.101 <0.490 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.503 <0.503 <0.503 <0.252 <0.252 <0.452 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.481 <0.252 <0.252 <0.252 <0.252 <0.252 1.79 [a] <0.101 <0.101 PFOA <0.240 <0.490 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.480 <0.480 <0.480 <0.480 <0.480 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.452 <0.240 <0.480 <0.480 <0.240 <0.240 <0.240 <0.481 <0.240 <0.240 <0.480 <0.480 <0.480 3.13 [a] <0.480 <0.480 PFBS <0.252 <0.980 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.905 <0.252 <0.503 <0.503 <0.252 <0.252 <0.252 <0.962 <0.252 <0.252 <1.10 <1.10 <1.10 0.887 [b] 0.460 1.31 PFHS <0.250 <0.980 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.905 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.962 <0.250 <0.250 <0.250 <0.250 <0.250 1.54 [a] <0.250 <0.250 PFOS 1.81 1.66 0.768 1.50 5.17 0.836 2.92 3.41 [b] 1.78 1.30 1.24 2.65 0.897 2.01 0.961 4.45 2.11 4.69 50.9 42.0 2.42 7.85 29.1 85.6 79.3 83.4 283 542 286 4.37 4.37 2.22 4.15 3.41 26.9 69.6 57.8 64.2 11.7 29.2 22.6 3.98 [b] 4.89 [b] 4.12 1.90 8.55 12.1 14.3 14.6 45.5 [b] 18.6 21.9 FBSA <0.250 ___ <0.250 <0.250 3.58 <0.250 0.295 0.512 [b] <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 0.575 3.37 3.56 0.609 1.39 1.29 69.2 65.8 44.8 37.5 34.1 33.3 1.16 1.90 0.867 1.16 0.633 72.8 [c] 65.9 [c] ___ 45.9 [c] 49.5 106 8.87 [c] 1.80 [c] 3.67 [c] ___ 2.5 [c] 3.97 [c] 96.3 [b,c] 52.4 [c] 55.5 [c] 19.6 [c] 69.2 [c] 84.2 [c] FOSA 0.153 [b] <0.588 <0.101 <0.101 0.277 [b] <0.101 0.196 [a] 0.123 [a] <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0.300 <0.101 <0.101 0.656 6.68 6.26 0.271 [a] 1.28 1.07 34.1 31.4 25.6 63.5 [b] 123 88.1 0.258 0.413 0.299 [a] <0.253 0.255 [a] 7.23 3.64 3.87 2.45 1.81 4.92 0.384 <0.253 <0.253 <0.577 <0.253 <0.253 4.81 2.35 2.40 6.38 4.20 3.01 N-MeFOSAA <0.100 ___ <0.100 <0.100 0.149 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 0.122 [a] 0.236 [b] 0.243 0.107 [a] <0.100 0.246 [b] 3.42 3.09 1.85 3.51 13.2 7.96 <0.100 0.121 <0.100 0.111 [a] <0.100 0.806 0.351 ___ 0.299 <0.500 0.731 <0.250 <0.250 <0.250 ___ <0.250 <0.250 0.829 0.654 [a] 0.696 [a] 2.4 [a] 1.94 0.729 N-EtFOSAA <0.250 ___ <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 2.66 2.50 1.16 1.00 4.30 3.32 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 ___ <0.250 <0.500 <0.500 <0.250 <0.250 <0.250 ___ <0.250 <0.250 0.511 <0.500 <0.500 4.73 [a] 0.844 0.281 Appendix B Table 2 Channel Catfish Fish Morphometries and PFC Concentrations Sample ID IPF007 IPF009 IPF034 IPF036 IPF153 IPF154D (IPF153) IPF002 IPF003 IPF039 IPF041 IPF155 IPF156D (IPF155) IPF018 IPF019D (IPF018) IPF022 IPF159 IPF160 IPF179 IPF024 IPF175 IPF176 IPF177D (IPF176) IPF178 IPF180 IPF047 IPF049 IPF051 IPF053 IPF055 IPF057 IPF059D (IPF057) IPF063 IPF066 IPF068 IPF069 IPF181 IPF182 DAL-IPF-LS-10002 DAL-IPF-LS-10004 DAL-IPF-LS-10005 Date 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/3/2012 11/21/2012 11/21/2012 11/3/2012 11/3/2012 11/3/2012 11/29/2012 11/29/2012 11/29/2012 11/3/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/29/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 11/7/2012 12/31/2012 12/31/2012 11/3/2012 11/3/2012 11/29/2012 Analytical Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental 3M Environmental AXYS Laboratory 3M Environmental 3M Environmental 3M Environmental Sampling Reach No. R10 R10 R10 R10 R10 R10 R11 R11 R11 R11 R11 R11 R12 R12 R12 R12 R12 R12 R13 R13 R13 R13 R13 R13 R14 R14 R14 R14 R14 R15 R15 R15 R15 R15 R15 Control Control Control Control Control Sampling Reach Browns Ferry Browns Ferry Browns Ferry Browns Ferry Browns Ferry Browns Ferry Mallard Creek Mallard Creek Mallard Creek Mallard Creek Mallard Creek Mallard Creek Elk River Elk River Elk River Elk River Elk River Elk River River Mile 281 River Mile 281 River Mile 281 River Mile 281 River Mile 281 River Mile 281 Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Big Nance Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek Shoal Creek NIST SAMPLE NIST SAMPLE na na na River Mile RM294 RM294 RM294 RM294 RM294 RM294 RM293.5 RM293.5 RM293.5 RM293.5 RM293.5 RM293.5 RM285 RM285 RM285 RM285 RM285 RM285 RM281 RM281 RM281 RM281 RM281 RM281 RM273 RM273 RM273 RM273 RM273 RM264 RM264 RM264 RM264 RM264 RM264 na na na na na Length (inches) 18 7/8 20 5/8 14 17 1/2 19 1/2 19 1/2 20 1/2 18 7/8 14 3/4 16 17 3/8 17 3/8 15 3/4 15 3/4 15 19 19 5/8 16 1/4 14 3/8 17 1/4 19 1/4 19 1/4 20 5/8 17 1/4 16 1/2 15 3/4 15 1/4 14 7/8 16 3/4 16 3/8 16 3/8 14 5/8 22 21 1/8 16 3/4 ----- 16 4/8 15 6/8 17 1/2 Weight (pounds) 2.0 3.4 0.8 1.9 2.5 2.5 2.2 1.3 0.9 1.1 1.7 1.7 1.3 1.3 0.8 1.9 2.4 1.3 0.9 1.9 2.1 2.1 2.9 1.4 1.5 1.0 0.7 0.9 1.6 1.2 1.2 0.9 3.7 2.7 0.8 ----- ------- PFBA <0.250 <0.250 <0.250 <0.250 <0.250 <0.503 <0.250 <0.250 <0.250 <0.250 <0.250 <0.472 <0.250 <0.483 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.100 <0.250 <0.250 <0.250 <0.250 <0.450 <0.250 <0.250 <0.250 PFHxA <0.0503 <0.0503 <0.101 <0.101 <0.252 <0.503 <0.0503 <0.0503 <0.101 <0.101 <0.252 <0.472 <0.0503 <0.483 <0.0503 <0.252 <0.252 <0.252 <0.101 <0.252 <0.252 0.421 <0.252 0.277 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.0503 <0.0503 <0.0503 <0.101 <0.450 <0.252 <0.252 <0.252 PFHpA <0.252 <0.252 <0.101 <0.101 <0.252 <0.503 <0.252 <0.252 <0.101 <0.101 <0.252 <0.472 <0.252 <0.483 <0.252 <0.252 <0.252 <0.252 <0.101 <0.252 <0.252 <0.252 <0.252 <0.252 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0.138 Tal <0.252 <0.450 <0.252 <0.252 <0.252 PFC Concentrations (ng/g; ppb) PFOA <0.480 <0.480 <0.480 <0.480 <0.480 <0.503 <0.480 <0.480 <0.480 <0.480 <0.480 <0.472 <0.480 <0.483 <0.480 <0.480 <0.480 <0.240 <0.480 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.240 <0.450 <0.240 <0.240 <0.240 PFBS <1.10 <1.10 1.862 0.371 [b] <0.503 < 1.01 <1.10 3.82 0.305 [b] 0.405 [b] <0.503 <0.943 <1.10 <0.966 <1.10 <0.503 <0.503 0.506 2.668 <0.252 <0.252 <0.252 0.322 <0.252 0.327 <0.252 0.337 1.54 [c] <0.252 <0.252 0.327 0.854 0.353 [b] <0.252 0.739 <0.252 <0.901 <0.252 <0.252 <0.252 PFHS <0.250 <0.250 <0.250 <0.250 <0.250 < 1.01 <0.250 <0.250 <0.250 <0.250 <0.250 <0.943 <0.250 <0.966 <0.250 <0.250 <0.250 <0.500 <0.250 <0.500 <0.500 <0.500 <0.500 <0.500 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.250 <0.901 <0.500 <0.500 <0.500 PFOS 8.86 13.4 8.12 14.4 16.3 14.5 8.49 18.3 15.7 22.2 11.9 12.3 11.7 10.2 5.10 11.2 4.87 10.7 10.3 8.57 11.8 12.7 10.5 7.69 14.3 5.59 8.01 6.68 11.0 7.33 7.56 6.67 10.5 [c] 9.60 5.63 5.00 5.72 <0.366 <0.366 <0.366 FBSA 4.83 [c] 5.29 [c] 5.38 [c] 7.86 [c] 10.1 --- 20.2 [c] 164 [b,c] 53.7 [c] 35.4 [c] 38.6 --- 28.6 [c] --- 36.9 [c] 20.4 8.47 35.7 12.3 [c] 36.2 28.6 29.9 51.2 27.8 63.9 20.6 28.0 15.9 31.5 22.8 26.1 16.0 37.5 40.8 18.0 <0.250 [c] --- <0.250 <0.250 <0.250 FOSA <0.505 0.487 0.884 [b] 0.629 0.816 <0.603 0.947 [b] 2.23 3.58 2.41 1.83 1.74 1.21 0.846 <0.505 0.620 0.490 0.670 1.07 [b] 1.36 1.54 1.60 1.23 1.43 2.71 1.29 1.71 1.31 1.37 [b] 1.58 1.69 1.43 1.95 1.48 0.771 <0.253 <0.541 <0.253 <0.253 <0.253 N-MeFOSAA <0.500 <0.500 <0.500 <0.500 <0.500 --- <0.500 1.07 0.716 1.30 <0.500 --- <0.500 --- <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 <0.500 0.539 0.293 <0.250 0.307 <0.250 0.333 [b] <0.250 <0.250 0.300 0.174 0.198 0.142 <0.250 --- <0.500 <0.500 <0.500 N-EtFOSAA <0.500 <0.500 <0.250 <0.250 <0.500 --- <0.500 0.714 0.373 0.381 <0.500 --- <0.500 --- <0.500 <0.500 <0.500 <0.500 0.26 [a] <0.500 <0.500 <0.500 <0.500 <0.500 0.116 [a] 0.141 [a] <0.100 <0.100 0.113 [b] 0.102 [a] <0.100 0.147 0.344 0.279 <0.250 <0.250 --- <0.500 <0.500 <0.500 Notes: P F C co n ce n tra tio n s a re th e a ve ra g e o f th e prim ary and d u p lic a te a n a lytica l re su lts e xp ressed in ng/g (ppb) on a w e t w e ig h t basis. S a m ple IDs w ith a "D " suffix are field dup lica tes obtained from left sid e filet sam ples. F ie ld d u p lic a te s a m p le s a re id e n tifie d w ith "D " s u ffix and th e c o rre s p o n d in g p rim a ry s a m p le ID in p a re n th e s e s . na - not applicable -- not supplied N R - S a m ple had LM S prepared and recovery w a s outside o f 100 + 50% and result not reported. * - q u a n tifie d fro m a lin e a r-P F O S (L -P F O S ) c a lib ra tio n fo r to ta l-P F O S (s u m o f lin e a r and b ra n c h e d is o m e r p e a k s ) in s a m p le s , L M S s and LC S s. [a] V a lue reported fo r single replicate results, dup lica te result w a s <LOQ. [b] R P D > 20% [c] S a m ple had LM S prepared and LM S recovery result w a s outsid e o f 100 + 30% , but w ith in 100 + 50% and w a s reported w ith an expa nded unce rtain ty o f + 50% . [d] V a lue reported fo r single result, dup lica te sam ple a na lysis had peak shift out o f retention tim e w in d o w and no result determ ined. D A L-IP F -L S -10002 = P re-S a m ple Filet; Lab T ag No. T N 12-0365-1/13 C a t_fish (250919) D A L-IP F -LS -10004 = Lab Tag No. T N 12-0365-2/12 C at_fish (250918) D A L-IP F -L S -10005 = P o st-S am ple Filet; Lab Tag No. T N 12-0385-1/12 C at_fish (250917) Post-analysis inspection of samples DAL-MSF-LS-10001 and DAL-IPF-LS-10002 indicated the samples had been switched. Table reflects correction. PFOS = P erfluorooctane sulfonate P F BA = P erfluo rob utan oic acid P F H xA = Perfluo roh exanoic acid P F H pA = P e rfluo roh epta noic acid PFO A = P erfluorooctanoic acid PFBS = P erfluorobutane sulfonate PFHS = P erfluorohexane sulfonate FB S A = P e rfluorobutanesulfonam ide F O S A =P erflu oro octan esulfona m ide N -M eFO S A A = 2-(N -ethyl-P FO S A ) acetic acid N -E tFO S A A = 2-(N -m ethyl-P F O S A ) acetic acid Sampling Reach No. R01 R02 R03 R04 R05 R06 R07 R08 R09 R10 R11 R12 Sampling Reach RM 320 Point Mallard RM 307.5 (Backwater) Betty Rye & Dry Branch Creek Bakers Creek Swan Creek RM 298 Round Island Creek Fox Creek Browns Ferry Mallard Creek Elk River Location Number L1 L2 L3 L1 L2 L3 L3 L3 L1 L2 L3 L1 L2 L3 L1 L1 L1 L2 L3 L1 L2 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L1 L2 L3 L3 L3 L1 L2 L3 L1 L2 L3 Sample ID DAL-SW-R01L1-0-121116 DAL-SW-R01L2-0-121116 DAL-SW-R01L3-0-121116 DAL-SW-R02L1-0-121116 DAL-SW-R02L2-0-121116 DAL-SW-R02L3-0-121116 DAL-SW-R02L3-DUP-121116 DAL-SW-R02L3-DUPA-121116 DAL-SW-R03L1-0-121116 DAL-SW-R03L2-0-121116 DAL-SW-R03L3-0-121116 DAL-SW-R04L1-0-121116 DAL-SW-R04L2-0-121116 DAL-SW-R04L3-0-121116 DAL-SW-R05L1-0-121119 DAL-SW-R05L1-DUP-121119 DAL-SW-R05L1-DUPA-121119 DAL-SW-R05L2-0-121119 DAL-SW-R05L3-0-121119 DAL-SW-R06L1-0-121120 DAL-SW-R06L2-0-121120 DAL-SW-R06L2-DUPA-121120 DAL-SW-R06L3-0-121120 DAL-SW-R07L1-0-121119 DAL-SW-R07L2-0-121119 DAL-SW-R07L3-0-121119 DAL-SW-R08L1-0-121119 DAL-SW-R08L2-0-121119 DAL-SW-R08L3-0-121119 DAL-SW-R09L1-0-121119 DAL-SW-R09L2-0-121119 DAL-SW-R09L3-0-121119 DAL-SW-R10L1-0-121119 DAL-SW-R10L2-0-121119 DAL-SW-R10L3-0-121119 DAL-SW-R10L3-DUP-121119 DAL-SW-R10L3-DUPA-121119 DAL-SW-R11L1-0-121119 DAL-SW-R11L2-0-121119 DAL-SW-R11L3-0-121119 DAL-SW-R12L1-0-121117 DAL-SW-R12L2-0-121117 DAL-SW-R12L3-0-121117 Date 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2010 11/20/2012 11/20/2012 11/20/2012 11/20/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/17/2012 11/17/2012 11/17/2012 Appendix B Table 3 Surface Water PFC Concentrations - Fish Reach Locations Time 10:45 11:15 11:40 12:50 13:05 13:20 13:20 13:20 13:50 14:00 14:08 14:45 15:00 15:10 16:25 16:25 16:25 16:35 16:45 16:15 16:30 16:30 16:40 15:10 15:25 15:40 14:05 14:20 14:35 11:40 11:50 12:05 10:35 10:48 11:00 11:00 11:00 9:35 9:45 10:00 16:20 16:35 16:50 Analytical Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory PFBA 6.16 6.50 <5.00 7.36 6.06 6.13 6.10 8.82 5.95 5.77 5.00 7.51 7.27 11.7 58.9 58.3 75.5 6.51 <5.00 <5.00 <5.00 6.18 <5.00 23.6 30.9 9.41 <5.00 <5.00 8.41 29.5 36.9 26.5 7.16 5.20 <5.00 <5.00 6.79 22.5 24.2 16.7 5.46 6.89 6.63 PFHxA 2.56 2.83 2.72 4.52 5.56 4.83 3.61 3.83 <2.00 <2.00 2.85 9.85 13.8 12.4 72.5 70.5 66.1 4.43 3.47 2.07 <2.00 1.62 3.30 34.4 41.4 10.9 2.40 <2.00 3.48 59.2 61.9 49.5 3.23 2.10 2.73 <2.00 1.64 75.5 70.2 47.7 7.41 7.56 6.61 PFHpA <2.00 <2.00 <2.00 3.47 3.64 4.70 3.78 4.64 <2.00 <2.00 <2.00 3.82 4.57 3.91 56.8 59.0 64.7 <2.00 <2.00 <2.00 <2.00 <1.21 <2.00 16.9 20.8 6.16 <2.00 <2.00 <2.00 22.1 25.4 18.1 <2.00 <2.00 <2.00 <2.00 1.24 19.5 22.6 12.0 <2.00 <2.00 <2.00 PFOA 2.16 3.04 2.31 10.7 10.4 9.28 9.12 9.14 3.43 3.70 3.34 13.6 15.0 31.7 246 242 210 9.02 5.82 2.97 <4.80 2.75 2.76 50.3 62.6 21.1 3.37 2.56 4.70 62.3 79.9 58.0 2.63 2.53 3.52 3.50 2.56 53.1 61.9 38.7 6.35 6.51 7.09 PFC Concentrations (ng/L; ppt) PFBS PFHS PFOS FBSA <5.00 <5.00 6.91 <2.00 <5.00 <5.00 7.62 <2.00 <5.00 <5.00 <5.00 <5.00 6.69 9.32 <2.00 <2.00 <5.00 7.03 9.06 <2.00 <5.00 <5.00 8.94 <2.00 <5.00 <5.00 8.46 <2.00 2.70 <5.00 <1.9 <5.00 7.59 7.03 --<2.00 <5.00 <5.00 7.34 <2.00 <5.00 <5.00 <5.00 <5.00 6.39 14.4 <2.00 2.14 <5.00 <5.00 18.0 2.07 10.4 11.0 70.5 4.86 124 39.8 385 1130 116 42.3 405 1050 166 35.5 388 -- <5.00 <5.00 17.7 <2.00 <5.00 <5.00 <5.00 <5.00 9.84 6.84 <2.00 <2.00 <5.00 <5.00 6.72 <2.00 2.69 <2.42 7.66 -- <5.00 46.4 <5.00 16.4 6.77 84.2 <2.00 245 63.8 19.5 102 352 9.65 <5.00 <5.00 <5.00 34.8 6.42 48.6 <2.00 <5.00 <5.00 6.57 <2.00 <5.00 64.1 <5.00 19.7 7.67 103 2.08 185 84.6 26.2 125 330 59.4 <5.00 19.6 <5.00 91.0 6.82 197 <2.00 <5.00 <5.00 6.99 <2.00 <5.00 <5.00 6.49 <2.00 <5.00 <5.00 6.63 <2.00 <2.15 52.1 <2.15 17.9 5.48 87.8 --129 51.1 18.9 91 116 28.9 <5.00 11.3 <5.00 69.5 12.4 74.1 4.75 <5.00 <5.00 13.8 4.95 <5.00 <5.00 13.3 5.12 FOSA N-MeFOSAA <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <0.952 <2.00 --<2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 21.1 18.0 20.3 -- <2.00 10.0 8.20 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <9.98 <1.21 -- <2.00 3.69 <2.00 2.14 5.65 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 6.15 <2.00 <2.00 7.71 <2.00 5.47 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <1.08 5.65 --<2.00 4.19 <2.00 3.89 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 N-EtFOSAA <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 --<5.00 <5.00 <5.00 <5.00 <5.00 <5.00 18.0 17.8 -- <5.00 <5.00 <5.00 <15.0 -- <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 --<5.00 <5.00 <5.00 <5.00 <5.00 <5.00 Page 1 of 2 Sampling Reach No. R13 R14 R15 Sampling Reach RM 281 Big Nance Creek Shoal Creek Location Number L1 L2 L3 L1 L2 L3 L1 L1 L1 L2 L3 Sample ID DAL-SW-R13L1-0-121117 DAL-SW-R13L2-0-121117 DAL-SW-R13L3-0-121117 DAL-SW-R14L1-0-121117 DAL-SW-R14L2-0-121117 DAL-SW-R14L3-0-121117 DAL-SW-R15L1-0-121117 DAL-SW-R15L1-DUP-121117 DAL-SW-R15L1-DUPA-121117 DAL-SW-R15L2-0-121117 DAL-SW-R15L3-0-121117 Date 11/17/2012 11/17/2012 11/17/2012 11/17/2012 11/17/2012 11/17/2012 11/17/2012 11/17/2012 11/17/2012 11/17/2012 11/17/2012 Notes: PFC concentrations are the average of the primary and duplicate analytical results expressed in in ng/L (ppt). DUP - Field duplicate sample (analyzed by the 3M Environmental Laboratory) DUPA - Field duplicate sample (analyzed by the AXYS Analytical Laboratory) -- Not Analyzed PFOS = Perfluorooctane sulfonate PFBA = Perfluorobutanoic acid PFHxA = Perfluorohexanoic acid PFHpA = Perfluoroheptanoic acid PFOA = Perfluorooctanoic acid PFBS = Perfluorobutane sulfonate PFHS = Perfluorohexane sulfonate FBSA = Perfluorobutanesulfonamide FOSA =Perfluorooctanesulfonamide N-MeFOSAA = 2-(N-ethyl-PFOSA) acetic acid N-EtFOSAA = 2-(N-methyl-PFOSA) acetic acid Appendix B Table 3 Surface Water PFC Concentrations - Fish Reach Locations Time 15:15 15:35 15:50 11:10 11:30 11:45 12:25 12:25 12:25 12:55 13:10 Analytical Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory PFBA 7.95 8.65 7.76 6.82 6.41 7.48 7.92 6.62 10.4 7.43 7.55 PFHxA 10.2 8.59 10.9 4.82 5.32 6.34 4.24 4.71 4.21 4.60 4.69 PFHpA 3.36 3.26 2.57 <2.00 2.18 2.19 2.34 <2.00 3.88 2.23 2.07 PFOA 14.4 18.2 15.3 11.1 11.5 12.0 11.5 10.9 10.6 12.8 10.7 PFC Concentrations (ng/L; ppt) PFBS PFHS PFOS FBSA 10.0 <5.00 24.8 20.4 12.0 <5.00 27.7 24.4 9.83 7.61 <5.00 <5.00 23.8 18.7 21.6 14.7 7.69 <5.00 19.0 14.3 8.47 14.8 13.7 20.9 <5.00 <5.00 <5.00 <2.2 23.0 19.9 18.9 22.0 16.1 34.8 31.8 -- 13.2 14.1 <5.00 <5.00 18.4 18.1 32.8 32.8 FOSA <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <1.1 <2.00 <2.00 N-MeFOSAA <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 -- <2.00 <2.00 N-EtFOSAA <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 -- <5.00 <5.00 Page 2 of 2 Sampling Reach No. T1 T2 T3 T4 Location Number 01 01 01 02 03 04 05 06 07 08 09 10 01 02 02 02 03 04 05 06 07 08 09 10 01 02 03 03 03 04 05 06 07 08 09 10 01 02 03 04 04 04 05 06 07 08 09 10 Sample ID DAL-SW-T101-0-121203 DAL-SW-T101-DUP-121203 DAL-SW-T101-DUPA-121203 DAL-SW-T102-0-121203 DAL-SW-T103-0-121203 DAL-SW-T104-0-121203 DAL-SW-T105-0-121203 DAL-SW-T106-0-121203 DAL-SW-T107-0-121203 DAL-SW-T108-0-121203 DAL-SW-T109-0-121203 DAL-SW-T110-0-121203 DAL-SW-T201-0-121203 DAL-SW-T202-0-121203 DAL-SW-T202-DUP-121203 DAL-SW-T202-DUPA-121203 DAL-SW-T203-0-121203 DAL-SW-T204-0-121203 DAL-SW-T205-0-121203 DAL-SW-T206-0-121203 DAL-SW-T207-0-121203 DAL-SW-T208-0-121204 DAL-SW-T209-0-121204 DAL-SW-T210-0-121204 DAL-SW-T301-0-121203 DAL-SW-T302-0-121203 DAL-SW-T303-0-121203 DAL-SW-T303-DUP-121203 DAL-SW-T303-DUPA-121203 DAL-SW-T304-0-121203 DAL-SW-T305-0-121203 DAL-SW-T306-0-121203 DAL-SW-T307-0-121203 DAL-SW-T308-0-121203 DAL-SW-T309-0-121203 DAL-SW-T310-0-121203 DAL-SW-T401-0-121203 DAL-SW-T402-0-121203 DAL-SW-T403-0-121203 DAL-SW-T404-0-121203 DAL-SW-T404-DUP-121203 DAL-SW-T404-DUPA-121203 DAL-SW-T405-0-121203 DAL-SW-T406-0-121203 DAL-SW-T407-0-121203 DAL-SW-T408-0-121203 DAL-SW-T409-0-121203 DAL-SW-T410-0-121203 Date 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/4/2012 12/4/2012 12/4/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 Time 8:40 8:40 8:40 9:00 9:15 9:30 9:45 9:55 10:05 10:10 10:15 10:25 9:30 9:10 9:10 9:10 8:50 8:30 9:50 10:05 10:15 10:05 11:10 10:45 12:20 12:10 11:50 11:50 11:50 12:35 12:45 12:55 13:02 13:08 13:10 13:18 15:55 16:15 14:05 14:50 14:50 14:50 15:08 15:25 15:37 15:22 15:10 14:55 Appendix B Table 4 Surface Water PFC Concentrations - Transect Locations Analytical Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory PFBA <5.00 <5.00 5.42 <5.00 <5.00 <5.00 <5.00 5.19 <5.00 <5.00 <5.00 <5.00 5.79 6.72 5.50 8.61 <5.00 <5.00 <5.00 <5.00 <5.00 5.66 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 5.91 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 38.1 5.98 7.83 <5.00 <5.00 6.09 <5.00 <5.00 <5.00 <5.00 5.04 5.67 PFHxA <2.00 <2.00 1.69 2.12 <2.00 2.18 2.16 <2.00 <2.00 2.00 <2.00 <2.00 4.75 5.16 4.58 4.93 2.20 <2.00 <2.00 <2.00 2.11 <2.00 <2.00 2.19 4.55 2.14 <2.00 <2.00 1.67 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 33.4 2.86 <2.00 <2.00 <2.00 1.58 <2.00 <2.00 <2.00 <2.00 <2.00 2.84 PFHpA <2.00 <2.00 1.16 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 3.07 4.80 3.64 6.01 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 2.23 <2.00 <2.00 <2.00 1.15 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 19.6 <2.00 <2.00 <2.00 <2.00 1.28 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 PFOA <1.92 <1.92 2.54 <1.92 2.51 2.55 2.56 2.57 <1.92 8.73 2.75 <1.92 9.73 12.6 10.7 10.5 <1.92 2.42 3.01 2.66 2.08 3.02 2.27 3.90 15.2 2.97 2.78 2.29 3.07 2.15 <1.92 <1.92 <1.92 <1.92 <1.92 2.28 74.5 4.54 2.73 2.25 <1.92 2.53 <1.92 <1.92 <1.92 <1.92 <1.92 3.87 PFC Concentrations (ng/L; ppt) PFBS PFHS PFOS FBSA <5.00 <5.00 <2.01 <5.00 <5.00 <2.01 6.13 6.36 5.26 <2.00 <2.00 --- <5.00 <5.00 6.15 <2.00 <5.00 <5.00 6.80 <2.00 <5.00 <5.00 6.89 <2.00 <5.00 <5.00 6.63 <2.00 <5.00 <5.00 6.43 <2.00 <5.00 <5.00 6.38 <2.00 <5.00 9.38 6.60 <2.00 <5.00 <5.00 6.45 <2.00 <5.00 <5.00 <5.00 <5.00 6.70 8.35 <2.00 <2.00 <5.00 <5.00 9.93 <2.00 <5.00 <5.00 9.43 <2.00 3.83 <2.03 8.44 --- <5.00 <5.00 6.50 <2.00 <5.00 <5.00 6.23 <2.00 <5.00 <5.00 6.96 <2.00 <5.00 <5.00 6.79 <2.00 <5.00 <5.00 6.83 <2.00 <5.00 <5.00 6.44 <2.00 <5.00 <5.00 6.98 <2.00 <5.00 <5.00 <5.00 <5.00 7.77 19.0 <2.00 2.32 <5.00 <5.00 7.46 <2.00 <5.00 <5.00 6.94 <2.00 <5.00 <5.00 7.51 <2.00 <2.07 <2.07 6.68 --- <5.00 <5.00 7.68 <2.00 <5.00 <5.00 7.30 <2.00 <5.00 <5.00 6.11 <2.00 <5.00 <5.00 6.57 <2.00 <5.00 <5.00 6.98 <2.00 <5.00 <5.00 6.58 <2.00 <5.00 285 <5.00 17.4 7.32 138 <2.00 453 <5.00 <5.00 8.69 2.81 <5.00 <5.00 7.52 <2.00 <5.00 <5.00 7.29 <2.00 <5.00 <2.37 <5.00 <2.37 8.46 6.30 <2.00 --- <5.00 <5.00 8.21 <2.00 <5.00 <5.00 7.42 <2.00 <5.00 <5.00 7.31 <2.00 <5.00 <5.00 7.04 <2.00 <5.00 <5.00 6.73 <2.00 <5.00 <5.00 7.78 <2.00 FOSA <2.00 <2.00 <1.01 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <1.02 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <1.04 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 18.3 <2.00 <2.00 <2.00 <2.00 <1.18 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 N-MeFOSAA <2.00 <2.00 --- <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 --<2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 --<2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 4.46 <2.00 <2.00 <2.00 <2.00 --- <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 N-EtFOSAA <5.00 <5.00 --- <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 --<5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 --<5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 --- <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 Page 1 of 3 Sampling Reach No. T5 T6 T7 T8 Location Number 01 02 03 04 05 05 05 06 07 08 09 10 01 02 03 04 05 06 06 06 06 07 08 09 10 01 02 03 04 05 06 07 07 07 08 09 10 01 02 03 04 05 06 07 08 08 08 09 10 Sample ID DAL-SW-T501-0-121204 Date 12/4/2012 DAL-SW-T502-0-121204 12/4/2012 DAL-SW-T503-0-121204 12/4/2012 DAL-SW-T504-0-121204 12/4/2012 DAL-SW-T505-0-121204 12/4/2012 DAL-SW-T505-DUP-121204 12/4/2012 DAL-SW-T505-DUPA-121204 12/4/2012 DAL-SW-T506-0-121204 12/4/2012 DAL-SW-T507-0-121204 12/4/2012 DAL-SW-T508-0-121204 12/4/2012 DAL-SW-T509-0-121204 12/4/2012 DAL-SW-T510-0-121204 DAL-SW-T601-0-121204 12/4/2012 12/4/2012 DAL-SW-T602-0-121204 12/4/2012 DAL-SW-T603-0-121204 12/4/2012 DAL-SW-T604-0-121204 12/4/2012 DAL-SW-T605-0-121204 12/4/2012 DAL-SW-T606-0-121204 12/4/2012 DAL-SW-T606-DUP-121204 12/4/2012 DAL-SW-T606-DUPA-121204 12/4/2012 DAL-SW-T606-DUPA-121204 (D 12/4/2012 DAL-SW-T607-0-121204 12/4/2012 DAL-SW-T608-0-121204 12/4/2012 DAL-SW-T609-0-121204 12/4/2012 DAL-SW-T610-0-121204 DAL-SW-T701-0-121204 12/4/2012 12/4/2012 DAL-SW-T702-0-121204 12/4/2012 DAL-SW-T703-0-121204 12/4/2012 DAL-SW-T704-0-121204 12/4/2012 DAL-SW-T705-0-121204 12/4/2012 DAL-SW-T706-0-121204 12/4/2012 DAL-SW-T707-0-121204 12/4/2012 DAL-SW-T707-DUP-121204 12/4/2012 DAL-SW-T707-DUPA-121204 12/4/2012 DAL-SW-T708-0-121204 12/4/2012 DAL-SW-T709-0-121204 12/4/2012 DAL-SW-T710-0-121204 DAL-SW-T801-0-121205 12/4/2012 12/5/2012 DAL-SW-T802-0-121205 12/5/2012 DAL-SW-T803-0-121205 12/5/2012 DAL-SW-T804-0-121205 12/5/2012 DAL-SW-T805-0-121205 12/5/2012 DAL-SW-T806-0-121205 12/5/2012 DAL-SW-T807-0-121205 12/5/2012 DAL-SW-T808-0-121205 12/5/2012 DAL-SW-T808-DUP-121205 12/5/2012 DAL-SW-T808-DUPA-121205 12/5/2012 DAL-SW-T809-0-121205 12/5/2012 DAL-SW-T810-0-121205 12/5/2012 Time 11:00 10:50 10:20 10:35 11:22 11:22 11:22 11:30 11:38 11:50 11:57 12:05 13:40 13:32 13:26 13:20 13:10 12:55 12:55 12:55 12:55 12:47 12:40 12:35 12:30 14:35 14:45 15:00 15:10 15:20 15:20 15:10 15:10 15:10 15:00 14:53 14:45 9:00 9:08 9:20 9:30 9:40 9:52 10:00 10:10 10:10 10:10 10:23 10:35 Appendix B Table 4 Surface Water PFC Concentrations - Transect Locations Analytical Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory 3M Laboratory PFBA 23.7 24.3 14.4 <5.00 <5.00 5.48 6.14 <5.00 <5.00 <5.00 5.02 8.56 43.2 23.3 13.9 8.09 7.48 <5.00 <5.00 6.01 5.71 <5.00 <5.00 5.72 8.74 18.1 15.7 11.4 8.59 9.71 10.1 8.42 7.66 9.21 6.96 7.35 8.39 6.89 6.82 9.03 7.76 8.01 5.79 6.26 6.28 6.89 8.89 6.50 5.62 PFHxA 26.7 24.5 13.7 <2.00 <2.00 <2.00 1.56 <2.00 <2.00 <2.00 <2.00 3.99 69.4 27.8 14.6 7.90 5.65 2.31 2.41 2.09 2.24 <2.00 <2.00 <2.00 2.75 17.5 15.8 12.3 8.17 9.49 7.39 4.05 4.53 4.65 3.90 4.20 5.25 4.58 4.33 3.80 4.46 3.92 3.80 2.95 4.51 3.85 3.61 2.80 2.79 PFHpA 14.4 14.2 9.19 <2.00 <2.00 <2.00 1.29 <2.00 <2.00 <2.00 <2.00 <2.00 27.3 22.0 9.62 4.85 3.37 <2.00 <2.00 2.11 B 1.88 B <2.00 <2.00 <2.00 <2.00 8.58 9.41 8.23 4.78 6.15 5.83 3.24 3.77 3.93 2.82 2.32 3.52 <2.00 <2.00 <2.00 2.81 <2.00 3.07 2.03 <2.00 <2.00 3.11 <2.00 <2.00 PFOA 48.2 45.7 29.0 <1.92 2.52 3.01 2.65 2.59 2.20 3.33 3.27 4.33 69.2 68.6 36.8 14.6 9.37 4.38 3.41 3.62 3.77 <1.92 <1.92 2.61 3.12 28.1 30.7 29.8 18.5 18.9 14.5 12.5 11.9 9.73 7.98 6.86 10.7 8.51 9.43 10.2 7.81 8.08 7.30 7.21 7.49 6.79 7.56 7.26 6.49 PFC Concentrations (ng/L; ppt) PFBS PFHS PFOS FBSA 105 14.3 72.8 139 115 12.6 76.5 168 63.8 8.35 49.2 96.4 <5.00 <5.00 7.89 <2.00 <5.00 <5.00 7.20 <2.00 <5.00 <2.23 <5.00 <2.23 6.78 5.10 <2.00 --- <5.00 <5.00 6.82 <2.00 <5.00 <5.00 8.10 <2.00 <5.00 <5.00 8.20 <2.00 <5.00 <5.00 8.15 <2.00 <5.00 89.6 <5.00 24.2 8.57 86.4 2.29 254 124 18.0 107 168 57.0 9.19 56.6 75.3 23.7 <5.00 23.8 32.1 12.4 <5.00 14.5 15.8 <5.00 <5.00 9.72 2.22 <5.00 <5.00 11.1 2.18 2.14 <2.01 7.45 -- <2.01 <2.01 8.62 -- <5.00 <5.00 6.49 <2.00 <5.00 <5.00 6.92 <2.00 <5.00 <5.00 7.66 <2.00 <5.00 71.0 <5.00 8.07 7.83 42.6 <2.00 213 65.1 8.37 46.6 118 55.6 7.69 46.8 75.1 32.5 <5.00 31.6 41.3 36.2 5.17 31.2 47.7 26.9 <5.00 24.2 35.6 19.0 <5.00 20.2 27.6 21.0 <5.00 19.3 26.4 15.0 <2.18 17.3 --- 14.0 <5.00 16.5 17.2 10.6 <5.00 13.4 12.6 17.7 <5.00 17.6 20.0 13.0 <5.00 15.2 32.2 12.5 <5.00 15.4 31.4 13.2 <5.00 15.9 34.8 14.3 <5.00 16.6 35.0 12.9 <5.00 15.4 35.0 12.1 <5.00 12.1 28.2 11.5 <5.00 14.1 27.0 11.2 <5.00 13.3 25.7 10.9 <5.00 14.3 25.7 11.4 <2.1 12.8 --- 9.28 <5.00 11.5 18.9 11.1 <5.00 12.3 14.1 FOSA 5.69 6.61 2.58 <2.00 <2.00 <2.00 <1.11 <2.00 <2.00 <2.00 <2.00 <2.00 6.17 5.06 3.06 <2.00 <2.00 <2.00 <2.00 <1 <1 <2.00 <2.00 <2.00 <2.00 4.77 2.86 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <1.09 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <1.05 <2.00 <2.00 N-MeFOSAA <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 --- <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 -- -- <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 --<2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 --- <2.00 <2.00 N-EtFOSAA <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 --- <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 -- -- <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 --<5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 --- <5.00 <5.00 Page 2 of 3 Sampling Reach No. T9 Location Number 01 02 03 04 05 06 07 08 09 09 09 10 Sample ID DAL-SW-T901-0-121205 DAL-SW-T902-0-121205 DAL-SW-T903-0-121205 DAL-SW-T904-0-121205 DAL-SW-T905-0-121205 DAL-SW-T906-0-121205 DAL-SW-T907-0-121205 DAL-SW-T908-0-121205 DAL-SW-T909-0-121205 DAL-SW-T909-DUP-0-121205 DAL-SW-T909-DUPA-0-121205 DAL-SW-T910-0-121205 Date 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 12/5/2012 Time 10:40 11:10 11:15 11:30 11:45 12:00 12:10 12:30 13:10 13:10 13:10 13:10 Appendix B Table 4 Surface Water PFC Concentrations - Transect Locations Analytical Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory 3M Laboratory AXYS Laboratory 3M Laboratory PFBA 7.76 9.51 7.45 8.98 7.48 6.76 7.69 6.74 8.66 8.97 8.91 7.96 PFHxA 9.09 7.72 7.62 7.82 8.78 6.57 7.25 6.58 7.12 6.28 5.42 6.42 PFHpA 3.32 3.88 2.16 3.28 3.11 3.30 3.99 3.43 3.37 3.75 3.73 3.37 PFOA 12.9 11.9 11.9 13.1 13.2 10.9 11.5 12.1 11.6 10.9 9.6 10.9 PFC Concentrations (ng/L; ppt) PFBS PFHS PFOS FBSA 17.7 <5.00 20.3 34.7 11.2 <5.00 20.5 35.1 14.2 <5.00 20.1 33.4 13.7 <5.00 20.7 36.9 14.7 <5.00 24.4 35.4 13.9 <5.00 19.7 43.2 12.7 <5.00 21.4 43.9 13.4 <5.00 20.3 45.4 12.8 <5.00 19.2 46.3 14.0 <5.00 19.4 45.6 12.6 <2.32 17.7 --- 13.4 <5.00 19.0 45.5 FOSA <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <1.16 <2.00 N-MeFOSAA <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 --- <2.00 N-EtFOSAA <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 --- <5.00 N o tes: P F C c o n c e n tra tio n s a re th e a v e ra g e o f th e p rim a ry a n d d u p lic a te a n a ly tic a l re s u lts e x p re s s e d in in n g /L (p p t). D U P - Field dup lica te sam ple (analyzed by th e 3M E n vironm ental Laboratory) D U P A - Field dup lica te sam ple (analyzed by th e A X Y S A n a lytica l Laboratory) -- Not A nalyzed PFO S = P e rfluo roo ctane sulfonate P F B A = P e rfluo rob utan oic acid P F H xA = P e rfluo roh exanoic acid P F H pA = P e rfluo roh epta noic acid P F O A = P erfluo roo ctano ic acid PFBS = P erfluo rob utan e sulfonate PFH S = P e rfluo roh exane sulfonate F B S A = P e rflu o ro b u ta n e su lfo n a m id e F O S A =P erflu oro octan esulfona m ide N -M eF O S A A = 2 -(N -ethyl-P F O S A ) acetic acid N -E tF O S A A = 2 -(N -m ethyl-P F O S A ) acetic acid Page 3 of 3 APPENDIX C 3M ENVIRONMENTAL LABORATORY ANALYTICAL DATA PACKAGES 3M Environmental Laboratory Report No. ISO12-01-01-03 Final Report Analysis of Water Samples from the Tennessee River - Fall 2012 Laboratory Request Number: IS012-01-01-03 Method Requirement: 3M Method ETS-8-044.1 Report Date - Date of Final Signature Testing Laboratory 3M Environmental, Health, Safety, and Sustainability Operations Environmental Laboratory 3M Center, Bldg 260-05-N-17 St. Paul, MN 55144 Requester Gary Hohenstein 3M Environmental, Health, Safety, and Sustainability Operations 3M Building 224-5W-03 Saint Paul, MN 55144-1000 Phone: (651)737-3570 ACCREDITED | T e s tin g C ert #2052.01 The testing reported herein meet the requirements o f ANSI/ ISO/IEC 17025:2005 " General Requirements for the Competence o f Testing and Calibration Laboratories" , in accordance with the A2LATesting Certificate # 2052.01. Testing that complies with this International Standard also meets principles o f ISO 9001:2000. PAGE 1 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 3M E nviro n m en tal Laboratory 3M Environm ental Laboratory M anager: W illiam K. R eagen, Ph.D. 3M Principal Analytical Investigator and Report Author: Susan W o lf Analytical Report IS012-01-01-03 Analysis of W a te r S am ples from the Tenn essee River - Fall 2 0 1 2 Report Date: D ata of Last Signature 1 Summary/Introduction T h e 3M Environmental Laboratory prepared sam ples collected by W eston personnel from the T en n e s s e e River, conducted per the study w ork plan dated O ctober 2 0 1 2 and titled "Revised W ork Plan for Fall 2 0 1 2 Fish an d S u rface W a te r S am p ling in th e T e n n e s s e e " in D ecatu r, A la b a m a . S am p les w ere collected from the fish sam pling reaches from N ovem ber 1 6 - 2 0 , 2 0 1 2 . S am ples w ere collected from the transect locations from D ecem ber 3-5, 2 0 1 2 . Equipm ent blank sam ples w ere collected N ovem ber 3 and 29, 20 1 2 . S am ples w ere returned to the 3M Environmental Laboratory on N ovem ber 6, 30 and D ecem b er 11, 201 2 , at am bient conditions and under 3 M Environmental Laboratory project n u m b er IS 0 1 2 -0 1 -0 1 -0 3 . All sam p le s w e re a n alyzed for P F B A , P F H x A , P F H p A , PFO A, PFBS, PFH S, PFO S, FBSA, FOSA, M eFO S A A and EtFOSAA. T h e 3M Environmental Laboratory prepared sam ple containers for forty-five (4 5 ) surface w ater locations associated with the fish sampling reaches, and ninety (9 0 ) surface w ater locations associated w ith the nine transect locations. E ach em p ty container w a s m arked with a "fill to h e re " line that corresponded to a final volum e of 2 0 0 m L. Single sam ple replicates w ere collected at each of the locations. Field sam ple duplicates w ere collected at 1 0 % of the sam pling locations (four (4) field duplicates collected at the fish sampling reaches and nine (9) field duplicates collected at the transect locations). In addition to e a c h o f th e field duplicate s am p les, at th o se s a m e locations, a targ et an alyte field matrix spike sam ple w a s also collected (four (4) target analyte field matrix spikes collected at the fish sam pling reaches and nine (9 ) target analyte field matrix spikes collected at the transect locations). All sam p les bottles included the addition o f internal standards (IS s) and surrogate recovery standards (S R S s ) 13C 3-P F B A , 13C 4-P F O A , an d 13C 4-P F O S , which w e re a d d e d to the s am p le containers prior to being sent to the field for sam ple collection. S am ple bottles reserved for field matrix spikes w ere fortified with solutions containing the target analytes, prior to being sent to the field for sam ple collection. S am p les w e re prepared and analyzed using direct injection with and without solvent dilution according to 3 M Environm ental Laboratory m etho d E T S -8 -0 4 4 .1 "M etho d o f A nalysis for th e D eterm ination of Perfluorinated C o m p o u n d s in W a te r by L C /M S /M S ; D irect Injection A nalysis". Internal standards w e re used to aid in th e d ata quality objectives. T a b le 1 s u m m arizes th e s a m p le results using th e analytical m ethod identified ab o ve. All results for quality control sa m p le s p rep ared and a n a ly ze d with th e sam p les will be reported and discussed els e w h e re in this report. The testing reported herein meet the requirements of ANSI/ISO/IEC 17025:2005 " General Requirements for the Competence o f Testing and Calibration Laboratories" , in accordance with the A2LA Testing Certificate # 2052.01. Testing that complies with this International Standard also meets principles o f ISO 9001:2000. T e s tin g C ert #2052.01 PAGE 2 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 1. Sample Results Summary ,1) 3M UMS ID Sample Description Fish Sampling Reaches ISO12-01-01-03-007 DAL-SW-R01L1-0-121116 ISO12-01-01-03-008 DAL-SW-R01L2-0-121116 ISO12-01-01-03-009 DAL-SW-R01L3-0-121116 ISO12-01-01-03-010 DAL-SW-R02L1-0-121116 IS012-01-01-03-011 DAL-SW-R02L2-0-121116 IS012-01-01-03-012 DAL-SW-R02L3-0-121116 IS012-01-01-03-015 DAL-SW-R03L1-0-121116 IS012-01-01-03-016 DAL-SW-R03L2-0-121116 IS012-01-01-03-017 DAL-SW-R03L3-0-121116 IS012-01-01-03-018 DAL-SW-R04L1-0-121116 IS012-01-01-03-019 DAL-SW-R04L2-0-121116 ISO12-01-01-03-020 DAL-SW-R04L3-0-121116 ISO12-01-01-03-052 DAL-SW-R05L1-0-121119 IS 0 12-01-01-03-055 DAL-SW-R05L2-0-121119 ISO12-01-01-03-056 DAL-SW-R05L3-0-121119 IS 0 12-01-01-03-057 DAL-SW-R06L1-0-121120 ISO12-01-01-03-058 DAL-SW-R06L2-0-121120 ISO12-01-01-03-059 DAL-SW-R06L3-0-121120 ISO12-01-01-03-049 DAL-SW-R07L1-0-121119 ISO12-01-01-03-050 DAL-SW-R07L2-0-121119 ISO12-01-01-03-051 DAL-SW-R07L3-0-121119 IS012-01-01-03-046 DAL-SW-R08L1-0-121119 ISO12-01-01-03-047 DAL-SW-R08L2-0-121119 ISO12-01-01-03-048 DAL-SW-R08L3-0-121119 PFBA PFHxA PFHpA Concentration (ng/L; parts per trillion) PFOA PFBS PFHS PFOS FBSA 6.16 2.56 <2.00 2.16 <5.00 <5.00 6.91 <2.00 6.50 2.83 <2.00 3.04 <5.00 <5.00 7.62 <2.00 <5.00 2.72 <2.00 2.31 <5.00 <5.00 6.69 <2.00 7.36 4.52 3.47 10.7 <5.00 <5.00 9.32 <2.00 6.06 5.56 3.64 10.4 <5.00 7.03 9.06 <2.00 6.13 4.83 4.70 9.28 <5.00 <5.00 8.94 <2.00 5.95 <2.00 <2.00 3.43 <5.00 <5.00 703 <2.00 5.77 <2.00 <2.00 3.70 <5.00 <5.00 7.34 <2.00 5.00 2.85 <2.00 3.34 <5.00 <5.00 6.39 <2.00 7.51 9.85 3.82 13.6 <5.00 <5.00 14.4 2.14 7.27 13.8 4.57 15.0 <5.00 <5.00 18.0 2.07 11.7 12.4 3.91 31.7 10.4 11.0 70.5 4.86 58.9 72.5 56.8 246 124 39.8 385 1130 6.51 4.43 <2.00 9.02 <5.00 <5.00 17.7 <2.00 <5.00 3.47 <2.00 5.82 <5.00 <5.00 9.84 <2.00 <5.00 2.07 <2.00 2.97 <5.00 <5.00 6.84 <2.00 <5.00 <2.00 <2.00 <4.80 <5.00 <5.00 6.72 <2.00 <5.00 3.30 <2.00 2.76 <5.00 <5.00 6.77 <2.00 23.6 34.4 16.9 50.3 46.4 16.4 84.2 245 30.9 41.4 20.8 62.6 63.8 19.5 102 352 9.41 10.9 6.16 21.1 9.65 <5.00 34.8 48.6 <5.00 2.40 <2.00 3.37 <5.00 <5.00 6.42 <2.00 <5.00 <2.00 <2.00 2.56 <5.00 <5.00 6.57 <2.00 8.41 3.48 <2.00 4.70 <5.00 <5.00 7.67 2.08 FOSA MeFOSAA EtFOSAA <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 21.1 <2.00 <2.00 <2.00 <2.00 <2.00 3.69 5.65 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 10.0 <2.00 <2.00 <2.00 <9.98 <2.00 2.14 <2.00 <2.00 <2.00 <2.00 <2.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 18.0 <5.00 <5.00 <5.00 <15.0 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 (1) All samples associated with the reported sampling location were analyzed using internal calibration. The analytical method uncertainties for the reported results are as follows: PFBA 16%, PFHxA 20%, PFHpA 21%, PFOA 23%, PFBS 20%, PFFIS 25% , PFOS 17%, FBSA 17%, FOSA 14%, MeFOSAA 25%, and EtFOSAA 17%. PAGE 3 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 1 continued. Sample Results Summary |1) 3M LIMS ID Sample Description Fish Sampling Reaches IS012-01-01-03-043 DAL-SW-R09L1 -0-121119 ISO12-01-01-03-044 DAL-SW-R09L2-0-121119 ISO12-01-01-03-045 DAL-SW-R09L3-0-121119 ISO12-01-01-03-038 DAL-SW-R10L1-0-121119 ISO12-01-01-03-039 DAL-SW-R10L2-0-121119 ISO12-01-01-03-040 DAL-SW-R10L3-0-121119 IS 0 12-01-01-03-035 DAL-SW-R11L1-0-121119 ISO12-01-01-03-036 DAL-SW-R11L2-0-121119 ISO12-01-01-03-037 DAL-SW-R11L3-0-121119 ISO12-01-01-03-032 DAL-SW-R12L1-0-121117 ISO12-01-01-03-033 DAL-SW-R12L2-0-121117 IS012-01-01-03-034 DAL-SW-R12L3-0-121117 ISO12-01-01-03-029 DAL-SW-R13L1-0-121117 ISO12-01-01-03-030 DAL-SW-R13L2-0-121117 ISO12-01-01-03-031 DAL-SW-R 13L3-0-121117 ISO12-01-01-03-021 DAL-SW-R14L1-0-121117 ISO12-01-01-03-022 DAL-SW-R14L2-0-121117 ISO12-01-01-03-023 DAL-SW-R 14L3-0-121117 ISO12-01-01-03-024 DAL-SW-R15L1-0-121117 ISO12-01-01-03-027 DAL-SW-R15L2-0-121117 ISO12-01-01-03-028 DAL-SW-R15L3-0-121117 PFBA PFHxA PFHpA Concentration (ng/L; parts per trillion) PFOA PFBS PFHS PFOS FBSA 29.5 59.2 22.1 62.3 64.1 19.7 103 185 36.9 61.9 25.4 79.9 84.6 26.2 125 330 26.5 49.5 18.1 58.0 59.4 19.6 91.0 197 7.16 3.23 <2.00 2.63 <5.00 <5.00 6.82 <2.00 5.20 2.10 <2.00 2.53 <5.00 <5.00 6.99 <2.00 <5.00 2.73 <2.00 3.52 <5.00 <5.00 6.49 <2.00 22.5 75.5 19.5 53.1 52.1 17.9 87.8 129 24.2 70.2 22.6 61.9 51.1 18.9 91.0 116 16.7 47.7 12.0 38.7 28.9 11.3 69.5 74.1 5.46 7.41 <2.00 6.35 <5.00 <5.00 12.4 4.75 6.89 7.56 <2.00 6.51 <5.00 <5.00 13.8 4.95 6.63 6.61 <2.00 7.09 <5.00 <5.00 13.3 5.12 7.95 10.2 3.36 14.4 10.0 <5.00 24.8 20.4 8.65 8.59 3.26 18.2 12.0 <5.00 27.7 24.4 7.76 10.9 2.57 15.3 9.83 <5.00 23.8 21.6 6.82 4.82 <2.00 11.1 7.61 <5.00 18.7 14.7 6.41 5.32 2.18 11.5 7.69 <5.00 19.0 14.3 7.48 6.34 2.19 12.0 8.47 <5.00 23.0 16.1 7.92 4.24 2.34 11.5 14.8 <5.00 19.9 34.8 7.43 4.60 2.23 12.8 13.2 <5.00 18.4 32.8 7.55 4.69 2.07 10.7 14.1 <5.00 18.1 32.8 FOSA MeFOSAA EtFOSAA 6.15 7.71 5.47 <2.00 <2.00 <2.00 5.65 4.19 3.89 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 (1) All samples associated with the reported sampling location were analyzed using internal calibration. The analytical method uncertainties for the reported results are as follows: PFBA 16%, PFHxA 20%, PFHpA 21%, PFOA 23%, PFBS 20%, PFHS 25%, PFOS 17%, FBSA 17%, FOSA 14%, MeFOSAA 25%, and EtFOSAA 17%. PAGE 4 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 1 continued. Sample Results Summary (11 3M LIMS ID Sample Description Transect Sampling Locations ISO12-01-01-03-062 DAL-SW-T101 -0-121203 ISO12-01-01-03-065 DAL-SW-T 102-0-121203 ISO12-01-01-03-066 DAL-SW-T103-0-121203 ISO12-01-01-03-067 DAL-SW-T104-0-121203 ISO12-01-01-03-068 DAL-SW-T105-0-121203 ISO12-01-01-03-069 DAL-SW-T106-0-121203 ISO12-01-01-03-070 DAL-SW-T107-0-121203 ISO12-01-01-03-071 DAL-SW-T108-0-121203 ISO12-01-01-03-072 DAL-SW-T109-0-121203 ISO12-01-01-03-073 DAL-SW-T110-0-121203 ISO12-01-01-03-074 DAL-SW-T201-0-121203 ISO12-01-01-03-075 DAL-SW-T202-0-121203 ISO12-01-01-03-078 DAL-SW-T203-0-121203 ISO12-01-01-03-079 DAL-SW-T204-0-121203 ISO12-01-01-03-080 DAL-SW-T205-0-121203 ISO12-01-01-03-081 DAL-SW-T206-0-121203 ISO12-01-01-03-082 DAL-SW-T207-0-121203 ISO12-01-01-03-083 DAL-SW-T208-0-121204 ISO12-01-01-03-084 DAL-SW-T209-0-121204 ISO12-01-01-03-085 DAL-SW-T210-0-121204 PFBA PFHxA PFHpA Concentration (ng/L; parts per trillion) PFOA PFBS PFHS PFOS FBSA FOSA MeFOSAA EtFOSAA <5.00 <5.00 <5.00 <5.00 <5.00 5.19 <5.00 <5.00 <5.00 <5.00 5.79 6.72 <5.00 <5.00 <5.00 <5.00 <5.00 5.66 <5.00 <5.00 <2.00 2.12 <2.00 2.18 2.16 <2.00 <2.00 2.00 <2.00 <2.00 4.75 5.16 2.20 <2.00 <2.00 <2.00 2.11 <2.00 <2.00 2.19 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 3.07 4.80 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <1.92 <1.92 2.51 2.55 2.56 2.57 <1.92 8.73 2.75 <1.92 9.73 12.6 <1.92 2.42 3.01 2.66 2.08 3.02 2.27 3.90 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 9.38 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 6.13 6.15 6.80 6.89 6.63 6.43 6.38 6.60 6.45 6.70 8.35 9.93 6.50 6.23 6.96 6.79 6.83 6.44 6.98 7.77 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 (1) All samples associated with the reported sampling location were analyzed using internal calibration. The analytical method uncertainties for the reported results are as follows: PFBA 16%, PFHxA 20%, PFHpA 21%, PFOA 23%, PFBS 20%, PFHS 25%, PFOS 17%, FBSA 17%, FOSA 14%, MeFOSAA 25%, and EtFOSAA 17%. PAGE 5 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 1 continued. Sample Results Summary (1) 3M UM S ID Sample Description Transect Sampling Locations ISO12-01-01-03-086 DAL-SW-T301 -0-121203 ISO12-01-01-03-087 DAL-SW-T302-0-121203 ISO12-01-01-03-088 DAL-SW-T303-0-121203 ISO12-01-01-03-091 DAL-SW-T304-0-121203 ISO12-01-01-03-092 DAL-SW-T305-0-121203 ISO12-01-01-03-093 DAL-SW-T306-0-121203 ISO12-01-01-03-094 DAL-SW-T307-0-121203 ISO12-01-01-03-095 DAL-SW-T308-0-121203 ISO12-01-01-03-096 DAL-SW-T309-0-121203 ISO12-01-01-03-097 DAL-SW-T310-0-121203 ISO12-01-01-03-098 DAL-SW-T401-0-121203 ISO12-01-01-03-099 DAL-SW-T402-0-121203 IS012-01-01-03-100 DAL-SW-T403-0-121203 IS012-01-01-03-101 DAL-SW-T404-0-121203 IS012-01-01-03-104 DAL-SW-T405-0-121203 IS012-01-01-03-105 DAL-SW-T406-0-121203 IS012-01-01-03-106 DAL-SW-T407-0-121203 IS012-01-01-03-107 DAL-SW-T408-0-121203 IS012-01-01-03-108 DAL-SW-T409-0-121203 IS012-01-01-03-109 DAL-SW-T410-0-121203 PFBA PFHxA PFHpA Concentration (ng/L; parts per trillion) PFOA PFBS PFHS PFOS FBSA FOSA MeFOSAA EtFOSAA <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 38.1 5.98 7.83 <5.00 <5.00 <5.00 <5.00 <5.00 5.04 5.67 4.55 2.14 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 33.4 2.86 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 2.84 2.23 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 19.6 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 15.2 2.97 2.78 2.15 <1.92 <1.92 <1.92 <1.92 <1.92 2.28 74.5 4.54 2.73 2.25 <1.92 <1.92 <1.92 <1.92 <1.92 3.87 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 285 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 17.4 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 19.0 7.46 6.94 7.68 7.30 6.11 6.57 6.98 6.58 7.32 138 8.69 7.52 7.29 8.21 7.42 7.31 7.04 6.73 7.78 2.32 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 453 2.81 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 18.3 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 4.46 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 (1) All samples associated with the reported sampling location were analyzed using internal calibration. The analytical method uncertainties for the reported results are as follows: PFBA 16%, PFHxA 20%, PFHpA 21%, PFOA 23%, PFBS 20%, PFHS 25%, PFOS 17%, FBSA 17%, FOSA 14%, MeFOSAA 25%, and EtFOSAA 17%. PAGE 6 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 1 continued. Sample Results Summary (11 3M LIMS ID Sample Description Transect Sampling Locations IS012-01-01-03-110 DAL-SW-T501 -0-121204 IS012-01-01-03-111 DAL-SW-T502-0-121204 IS012-01-01-03-112 DAL-SW-T503-0-121204 IS012-01-01-03-113 DAL-SW-T504-0-121204 IS012-01-01-03-114 DAL-SW-T505-0-121204 IS012-01-01-03-117 DAL-SW-T506-0-121204 IS012-01-01-03-118 DAL-SW-T507-0-121204 IS012-01-01-03-119 DAL-SW-T508-0-121204 IS012-01-01-03-120 DAL-SW-T509-0-121204 IS012-01-01-03-121 DAL-SW-T510-0-121204 IS012-01-01-03-122 DAL-SW-T601-0-121204 IS012-01-01-03-123 DAL-SW-T602-0-121204 IS012-01-01-03-124 DAL-SW-T603-0-121204 IS012-01-01-03-125 DAL-SW-T604-0-121204 IS012-01-01-03-126 DAL-SW-T605-0-121204 IS012-01-01-03-127 DAL-SW-T606-0-121204 IS012-01-01-03-130 DAL-SW-T607-0-121204 IS012-01-01-03-131 DAL-SW-T608-0-121204 IS012-01-01-03-132 DAL-SW-T609-0-121204 IS012-01-01-03-133 DAL-SW-T610-0-121204 PFBA PFHxA PFHpA Concentration (ng/L; parts per trillion) PFOA PFBS PFHS PFOS FBSA 23.7 26.7 14.4 48.2 105 14.3 72.8 139 24.3 24.5 14.2 45.7 115 12.6 76.5 168 14.4 13.7 9.19 29.0 63.8 8.35 49.2 96.4 <5.00 <2.00 <2.00 <1.92 <5.00 <5.00 7.89 <2.00 <5.00 <2.00 <2.00 2.52 <5.00 <5.00 7.20 <2.00 <5.00 <2.00 <2.00 2.59 <5.00 <5.00 6.82 <2.00 <5.00 <2.00 <2.00 2.20 <5.00 <5.00 8.10 <2.00 <5.00 <2.00 <2.00 3.33 <5.00 <5.00 8.20 <2.00 5.02 <2.00 <2.00 3.27 <5.00 <5.00 8.15 <2.00 8.56 3.99 <2.00 4.33 <5.00 <5.00 8.57 2.29 43.2 69.4 27.3 69.2 89.6 24.2 86.4 254 23.3 27.8 22.0 68.6 124 18.0 107 168 13.9 14.6 9.62 36.8 57.0 9.19 56.6 75.3 8.09 7.90 4.85 14.6 23.7 <5.00 23.8 32.1 7.48 5.65 3.37 9.37 12.4 <5.00 14.5 15.8 <5.00 2.31 <2.00 4.38 <5.00 <5.00 9.72 2.22 <5.00 <2.00 <2.00 <1.92 <5.00 <5.00 6.49 <2.00 <5.00 <2.00 <2.00 <1.92 <5.00 <5.00 6.92 <2.00 5.72 <2.00 <2.00 2.61 <5.00 <5.00 7.66 <2.00 8.74 2.75 <2.00 3.12 <5.00 <5.00 7.83 <2.00 FOSA MeFOSAA EtFOSAA 5.69 6.61 2.58 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 6.17 5.06 3.06 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 (1) All samples associated with the reported sampling location were analyzed using internal calibration. The analytical method uncertainties for the reported results are as follows: PFBA 16%, PFHxA 20%, PFHpA 21%, PFOA 23%, PFBS 20%, PFHS 25%, PFOS 17%, FBSA 17%, FOSA 14%, MeFOSAA 25%, and EtFOSAA 17%. PAGE 7 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 1 continued. Sample Results Summary (1) 3M UM S ID Sample Description Transect Sampling Locations IS012-01-01-03-134 DAL-SW-T701 -0-121204 IS012-01-01-03-135 DAL-SW-T702-0-121204 IS012-01-01-03-136 DAL-SW-T703-0-121204 IS012-01-01-03-137 DAL-SW-T704-0-121204 IS012-01-01-03-138 DAL-SW-T705-0-121204 IS012-01-01-03-139 DAL-SW-T706-0-121204 ISO12-01-01-03-140 DAL-SW-T707-0-121204 ISO12-01-01-03-143 DAL-SW-T708-0-121204 IS012-01-01-03-144 DAL-SW-T709-0-121204 ISO12-01-01-03-145 DAL-SW-T710-0-121204 IS012-01-01-03-146 DAL-SW-T801-0-121205 ISO12-01-01-03-147 DAL-SW-T802-0-121205 IS012-01-01-03-148 DAL-SW-T803-0-121205 IS012-01-01-03-149 DAL-SW-T804-0-121205 IS012-01-01-03-150 DAL-SW-T805-0-121205 IS012-01-01-03-151 DAL-SW-T806-0-121205 IS012-01-01-03-152 DAL-SW-T807-0-121205 IS012-01-01-03-153 DAL-SW-T808-0-121205 IS012-01-01-03-156 DAL-SW-T809-0-121205 IS012-01-01-03-157 DAL-SW-T810-0-121205 PFBA PFHxA PFHpA Concentration (ng/L; parts per trillion) PFOA PFBS PFHS PFOS FBSA FOSA MeFOSAA EtFOSAA 18.1 15.7 11.4 8.59 9.71 10.1 8.42 6.96 7.35 8.39 6.89 6.82 9.03 7.76 8.01 5.79 6.26 6.28 6.50 5.62 17.5 15.8 12.3 8.17 9.49 7.39 4.05 3.90 4.20 5.25 4.58 4.33 3.80 4.46 3.92 3.80 2.95 4.51 2.80 2.79 8.58 9.41 8.23 4.78 6.15 5.83 3.24 2.82 2.32 3.52 <2.00 <2.00 <2.00 2.81 <2.00 3.07 2.03 <2.00 <2.00 <2.00 28.1 30.7 29.8 18.5 18.9 14.5 12.5 7.98 6.86 10.7 8.51 9.43 10.2 7.81 8.08 7.30 7.21 7.49 7.26 6.49 71.0 65.1 55.6 32.5 36.2 26.9 19.0 14.0 10.6 17.7 13.0 12.5 13.2 14.3 12.9 12.1 11.5 11.2 9.28 11.1 8.07 8.37 7.69 <5.00 5.17 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 42.6 46.6 46.8 31.6 31.2 24.2 20.2 16.5 13.4 17.6 15.2 15.4 15.9 16.6 15.4 12.1 14.1 13.3 11.5 12.3 213 118 75.1 41.3 47.7 35.6 27.6 17.2 12.6 20.0 32.2 31.4 34.8 35.0 35.0 28.2 27.0 25.7 18.9 14.1 4.77 2.86 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 (1) All samples associated with the reported sampling location were analyzed using internal calibration. The analytical method uncertainties for the reported results are as follows: PFBA 16%, PFHxA 20%, PFHpA 21%, PFOA 23%, PFBS 20%, PFHS 25%, PFOS 17%, FBSA 17%, FOSA 14%, MeFOSAA 25%, and EtFOSAA 17%. PAGE 8 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 1 continued. Sample Results Summary (1) 3M UM S ID Sample Description Transect Sampling Locations IS012-01-01-03-158 DAL-SW-T901 -0-121205 IS012-01-01-03-159 DAL-SW-T902-0-121205 IS012-01-01-03-160 DAL-SW-T903-0-121205 IS012-01-01-03-161 DAL-SW-T904-0-121205 IS012-01-01-03-162 DAL-SW-T905-0-121205 IS012-01-01-03-163 DAL-SW-T906-0-121205 IS012-01-01-03-164 DAL-SW-T907-0-121205 IS012-01-01-03-165 DAL-SW-T908-0-121205 IS012-01-01-03-166 DAL-SW-T909-0-121205 IS012-01-01-03-169 DAL-SW-T910-0-121205 Trip and Equipment Blanks ISO12-01-01-03-001 DAL-MSW-LS-EQB01 ISO12-01-01-03-002 DAL-MSW-LS-EQB02 ISO12-01-01-03-003 DAL-MSW-LS-EQB03 ISO12-01-01-03-060 DAL-SW-TRIP01-0-121116 IS012-01-01-03-170 DAL-SW-TRI P-01-0-121203 IS012-01-01-03-172 DAL-IPW-LS-EQB04 PFBA PFHxA PFHpA Concentration (ng/L; parts per trillion) PFOA PFBS PFHS PFOS FBSA FOSA MeFOSAA EtFOSAA 7.76 9.51 7.45 8.98 7.48 6.76 7.69 6.74 8.66 7.96 9.09 7.72 7.62 7.82 8.78 6.57 7.25 6.58 7.12 6.42 3.32 3.88 2.16 3.28 3.11 3.30 3.99 3.43 3.37 3.37 12.9 11.9 11.9 13.1 13.2 10.9 11.5 12.1 11.6 10.9 17.7 11.2 14.2 13.7 14.7 13.9 12.7 13.4 12.8 13.4 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 20.3 20.5 20.1 20.7 24.4 19.7 21.4 20.3 19.2 19.0 34.7 35.1 33.4 36.9 35.4 43.2 43.9 45.4 46.3 45.5 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <1.92 <1.92 <1.92 <1.92 <1.92 <1.92 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 <0.927 1.05 <0.927 <0.927 <0.927 <0.927 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <2.00 <5.00 <5.00 <5.00 <5.00 <5.00 <5.00 (1) All samples associated with the reported sampling location were analyzed using internal calibration. The analytical method uncertainties for the reported results are as follows: PFBA 16%, PFHxA 20%, PFHpA 21%, PFOA 23%, PFBS 20%, PFHS 25%, PFOS 17%, FBSA 17%, FOSA 14%, MeFOSAA 25%, and EtFOSAA 17%. PAGE 9 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 2. Surrogate Recovery Standards Summary SRS Recovery (%) 3M UM S ID Sample Description Fish Sampling Reaches IS 012-01-01-03-007 DAL-SW-R01L1-0-121116 IS 012-01-01-03-008 DAL-SW-R01L2-0-121116 IS 012-01-01-03-009 DAL-SW-R01L3-0-121116 IS 012-01-01-03-010 DAL-SW-R02L1-0-121116 IS 012-01-01-03-011 DAL-SW-R02L2-0-121116 IS 012-01-01-03-012 DAL-SW-R02L3-0-121116 IS 012-01-01-03-013 DAL-SW-R02L3-DB-121116 IS 012-01-01-03-014 DAL-SW-R02L3-FMS-12 1116 IS 012-01-01-03-015 DAL-SW-R03L1-0-121116 IS 012-01-01-03-016 DAL-SW-R03L2-0-121116 IS 012-01-01-03-017 DAL-SW-R03L3-0-121116 IS 012-01-01-03-018 DAL-SW-R04L1-0-121116 IS 012-01-01-03-019 DAL-SW-R04L2-0-121116 IS 012-01-01-03-020 DAL-SW-R04L3-0-121116 IS 0 12-01-01-03-052 DAL-SW-R05L1-0-121119 IS 0 12-01-01-03-053 DAL-SW-R05L1-DB-121119 IS 0 12-01-01-03-054 DAL-SW-R05L1 -FMS-121119 IS 0 12-01-01-03-055 DAL-SW-R05L2-0-121119 IS 0 12-01-01-03-056 DAL-SW-R05L3-0-121119 IS 0 12-01-01-03-057 DAL-SW-R06L1-0-121120 IS 0 12-01-01-03-058 DAL-SW-R06L2-0-121120 IS 0 12-01-01-03-059 DAL-SW-R06L3-0-121120 IS012-01-01-03-049 DAL-SW-R07L1-0-121119 IS 0 12-01-01-03-050 DAL-SW-R07L2-0-121119 IS 0 12-01-01-03-051 DAL-SW-R07L3-0-121119 IS012-01-01-03-046 DAL-SW-R08L1-0-121119 IS 012-01-01-03-047 DAL-SW-R08L2-0-121119 IS 012-01-01-03-048 DAL-SW-R08L3-0-121119 IS012-01-01-03-043 DAL-SW-R09L1-0-121119 IS 012-01-01-03-044 DAL-SW-R09L2-0-121119 IS 012-01-01-03-045 DAL-SW-R09L3-0-121119 IS 0 12-01-01-03-038 DAL-SW-R10L1-0-121119 IS 0 12-01-01-03-039 DAL-SW-R10L2-0-121119 IS 012-01-01-03-040 DAL-SW-R10L3-0-121119 IS 012-01-01-03-041 DAL-SW-R10L3-DB-121119 IS 012-01-01-03-042 DAL-SW-R10L3-FMS-121119 IS 0 12-01-01-03-035 DAL-SW-R11L1-0-121119 IS 0 12-01-01-03-036 DAL-SW-R11L2-0-121119 IS 0 12-01-01-03-037 DAL-SW-R11L3-0-121119 IS 0 12-01-01-03-032 DAL-SW-R12L1-0-121117 IS 0 12-01-01-03-033 DAL-SW-R12L2-0-121117 ISO12-01-01-03-034 DAL-SW-R12L3-0-121117 13c 3-p f b a 90.6 93.8 93.8 93.8 94.2 94.8 926 93.6 89.8 922 95.6 90.6 922 928 93.6 91 2 922 928 91.4 88.2 94.2 89.4 90.4 96.0 99.4 94.6 95.0 92.6 89.8 922 95.8 91.4 928 90.0 93.0 90.8 90.2 94.4 94.2 90.2 95.2 96.0 13C4-PFOA 93.0 82.4 83.4 87.2 87.0 82.0 85.0 87.4 87.2 81.4 86.6 85.4 83.8 84.4 82.4 83.0 87.4 84.8 84.6 85.2 85.6 86.2 82.8 86.2 86.4 83.8 85.4 84.2 85.4 83.6 83.6 80.0 83.8 89.0 86.6 84.0 79.0 83.2 85.2 76.6 85.8 87.4 13C,,-PFOS 89.1 91.4 91.8 88.5 90.6 90.8 91.0 89.5 91.1 926 92.0 929 94.8 90.8 89.1 929 91.6 91.4 99.4 90.1 89.4 91.5 96.9 88.3 97.9 87.7 89.7 90.0 95.6 95.0 973 91.6 89.7 91.8 91 2 91.8 94.1 89.7 90.0 89.3 937 95.6 PAGE 10 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 2 continued. Surrogate Recovery Standards Summary SRS Recovery (%) 3M UM S ID Sample Description Fish Sampling Reaches IS 012-01-01-03-029 DAL-SW-R13L1-0-121117 IS012-01-01-03-030 DAL-SW-R13L2-0-121117 IS 012-01-01-03-031 DAL-SW-R13L3-0-121117 IS 0 12-01-01-03-021 DAL-SW-R14L1-0-121117 IS 0 12-01-01-03-022 DAL-SW-R14L2-0-121117 IS 0 12-01-01-03-023 DAL-SW-R14L3-0-121117 IS 0 12-01-01-03-024 DAL-SW-R15L1-0-121117 IS 0 12-01-01-03-025 DAL-SW-R15L1-DB-121117 IS 0 12-01-01-03-026 DAL-SW-R15L1-FMS-121117 IS 0 12-01-01-03-027 DAL-SW-R15L2-0-121117 ISO12-01-01-03-028 DAL-SW-R15L3-0-121117 Transect Sampling Locations IS012-01-01-03-062 DAL-SW-T101 -0-121203 IS 0 12-01-01-03-063 DAL-SW-T101 -DB-121203 IS012-01-01-03-064 DAL-SW-T101-FMS-121203 IS 0 12-01-01-03-065 DAL-SW-T102-0-121203 IS 0 12-01-01-03-066 DAL-SW-T103-0-121203 IS 0 12-01-01-03-067 DAL-SW-T104-0-121203 IS 0 12-01-01-03-068 DAL-SW-T105-0-121203 IS 0 12-01-01-03-069 DAL-SW-T106-0-121203 IS012-01-01-03-070 DAL-SW-T107-0-121203 IS 0 12-01-01-03-071 DAL-SW-T108-0-121203 IS012-01-01-03-072 DAL-SW-T109-0-121203 IS012-01-01-03-073 DAL-SW-T110-0-121203 IS012-01-01-03-074 DAL-SW-T201-0-121203 IS 0 12-01-01-03-075 DAL-SW-T202-0-121203 IS012-01-01-03-076 DAL-SW-T202-DB-121203 IS 0 12-01-01-03-077 DAL-SW-T202-FMS-121203 IS 0 12-01-01-03-078 DAL-SW-T203-0-121203 IS 0 12-01-01-03-079 DAL-SW-T204-0-121203 IS 0 12-01-01-03-080 DAL-SW-T205-0-121203 IS 0 12-01-01-03-081 DAL-SW-T206-0-121203 IS 0 12-01-01-03-082 DAL-SW-T207-0-121203 IS 0 12-01-01-03-083 DAL-SW-T208-0-121204 IS 012-01-01-03-084 DAL-SW-T209-0-121204 ISO12-01-01-03-085 DAL-SW-T210-0-121204 13c 3-p f b a 88.8 926 92.8 95.4 95.4 94.0 90.4 93.1 93.0 94.4 91.8 94.2 96.2 93.8 922 926 93.0 96.6 95.6 96.6 95.0 926 94.6 97.0 94.4 93.6 99.8 94.2 94.0 94.6 95.0 91.6 952 97.4 96.8 13C4-PFOA 79.6 82.2 84.4 81.8 88.0 89.8 85.6 86.8 86.0 83.8 84.0 86.4 86.4 822 86.4 91 2 922 87.8 88.0 84.8 86.6 92.4 90.0 89.0 91.0 91.4 88.2 84.4 87.8 90.6 83.8 83.4 92.0 85.2 926 13C,,-PFOS 92 1 91.2 94.4 939 92.5 90.2 94.1 93.7 95.2 91 2 88.5 91.0 90.0 94.8 94.4 923 90.0 91.8 92.5 91.8 89.1 91 2 94.1 92 1 92 1 90.8 92 1 91.4 929 923 929 93.7 927 929 86.8 PAGE 11 OF 52 3M Environmental Laboratory Report No. IS012-01-01-03 Table 2 continued. Surrogate Recovery Standards Summary 3M UMS ID Sample Description Transect Sampling Locations IS 0 1 2-01-01-03-086 IS 0 1 2-01-01-03-087 IS 0 1 2-01-01-03-088 IS 0 12-01-01-03-089 IS 0 12-01-01-03-090 IS 0 12-01-01-03-091 IS 0 12-01-01-03-092 IS 0 12-01-01-03-093 IS 0 12-01-01-03-094 IS 0 12-01-01-03-095 IS 0 12-01-01-03-096 IS 0 1 2-01-01-03-097 D A L -S W -T 3 0 1-0-121203 DAL-SW-T302-0-121203 DAL-SW-T303-0-121203 DAL-SW-T303-DB-121203 DAL-SW-T303-FMS-121203 DAL-SW-T304-0-121203 DAL-SW-T305-0-121203 DAL-SW-T306-0-121203 DAL-SW-T307-0-121203 DAL-SW-T308-0-121203 DAL-SW-T309-0-121203 DAL-SW-T310-0-121203 IS 0 12-01-01-03-098 IS 0 12-01-01-03-099 IS 0 1 2-01-01-03-100 IS 012-01-01-03-101 IS 0 1 2-01-01-03-102 IS 0 1 2-01-01-03-103 IS 0 1 2-01-01-03-104 IS 0 1 2-01-01-03-105 IS 0 1 2-01-01-03-106 IS 0 1 2-01-01-03-107 IS 0 1 2-01-01-03-108 IS 0 1 2-01-01-03-109 IS012-01-01-03-110 IS012-01-01-03-111 IS012-01-01-03-112 IS012-01-01-03-113 IS012-01-01-03-114 IS012-01-01-03-115 IS012-01-01-03-116 IS012-01-01-03-117 IS012-01-01-03-118 IS012-01-01-03-119 IS 0 1 2-01-01-03-120 ISO12-01-01-03-121 DAL-SW-T401-0-121203 DAL-SW-T402-0-121203 DAL-SW-T403-0-121203 DAL-SW-T404-0-121203 DAL-SW-T404-DB-121203 DAL-SW-T404-FMS-121203 DAL-SW-T405-0-121203 DAL-SW-T406-0-121203 DAL-SW-T407-0-121203 DAL-SW-T408-0-121203 DAL-SW-T409-0-121203 DAL-SW-T410-0-121203 DAL-SW-T501-0-121204 DAL-SW-T502-0-121204 DAL-SW-T503-0-121204 DAL-SW-T504-0-121204 DAL-SW-T505-0-121204 DAL-SW-T505-DB-121204 DAL-SW-T505-FMS-121204 DAL-SW-T506-0-121204 DAL-SW-T507-0-121204 DAL-SW-T508-0-121204 DAL-SW-T509-0-121204 DAL-SW-T510-0-121204 SRS Recovery (%) 13C3-PFBA 13C,,-PFOA 13C4-PFOS 96.4 94.8 91.2 94.2 93.0 94.6 96.2 95.0 94.6 93.0 93.8 95.6 95.4 96.6 94.4 96.0 922 93.8 94.0 928 95.0 94.0 91.4 94.8 952 94.8 972 96.4 94.6 952 94.2 96.0 96.0 93.8 96.4 932 90.0 87.6 90.2 89.6 85.8 90.4 85.4 91 2 89.6 84.6 86.6 88.8 87.0 85.4 91.6 87.8 92.0 88.8 88.0 90.6 81.8 930 86.2 90.0 89.6 88.2 91 2 86.8 88.0 84.6 86.4 88.6 85.2 91.6 85.4 91.4 93.3 95.0 93.3 92.5 94.1 92.5 90.6 90.6 91.6 91.4 90.8 90.6 91.8 92 1 91.0 94.6 91.6 939 90.6 91.6 90.4 92.5 91 2 89.3 97.9 95.8 973 933 93.5 939 939 90.8 91.0 94.1 90.6 91.4 PAGE 12 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 2 continued. Surrogate Recovery Standards Summary 3M UMS ID Sample Description Transect Sampling Locations IS 0 1 2-01-01-03-122 IS 0 1 2-01-01-03-123 IS 0 1 2-01-01-03-124 D A L -S W -T 6 0 1-0-121204 DAL-SW-T602-0-121204 DAL-SW-T603-0-121204 IS012-01-01-03-125 DAL-SW-T604-0-121204 IS 0 1 2-01-01-03-126 IS 0 1 2-01-01-03-127 IS 0 1 2-01-01-03-128 DAL-SW-T605-0-121204 DAL-SW-T606-0-121204 DAL-SW-T606-DB-121204 IS 0 1 2-01-01-03-129 IS 0 1 2-01-01-03-130 IS 012-01-01-03-131 DAL-SW-T606-FMS-121204 DAL-SW-T607-0-121204 DAL-SW-T608-0-121204 IS012-01-01-03-132 DAL-SW-T609-0-121204 IS012-01-01-03-133 DAL-SW-T610-0-121204 IS 012-01-01-03-134 DAL-SW-T701-0-121204 IS 0 1 2-01-01-03-135 IS 0 1 2-01-01-03-136 IS 0 1 2-01-01-03-137 DAL-SW-T702-0-121204 DAL-SW-T703-0-121204 DAL-SW-T704-0-121204 IS 0 1 2-01-01-03-138 IS 0 1 2-01-01-03-139 IS 0 1 2-01-01-03-140 DAL-SW-T705-0-121204 DAL-SW-T706-0-121204 DAL-SW-T707-0-121204 IS 012-01-01-03-141 DAL-SW-T707-DB-121204 IS 0 1 2-01-01-03-142 IS 0 1 2-01-01-03-143 IS 0 1 2-01-01-03-144 DAL-SW-T707-FMS-121204 DAL-SW-T708-0-121204 DAL-SW-T709-0-121204 IS012-01-01-03-145 DAL-SW-T710-0-121204 IS012-01-01-03-146 DAL-SW-T801-0-121205 IS 0 1 2-01-01-03-147 IS 0 1 2-01-01-03-148 IS 0 1 2-01-01-03-149 DAL-SW-T802-0-121205 DAL-SW-T803-0-121205 DAL-SW-T804-0-121205 IS 012-01-01-03-150 DAL-SW-T805-0-121205 IS 012-01-01-03-151 IS 0 1 2-01-01-03-152 IS 0 1 2-01-01-03-153 DAL-SW-T806-0-121205 DAL-SW-T807-0-121205 DAL-SW-T808-0-121205 IS 0 1 2-01-01-03-154 IS 0 1 2-01-01-03-155 IS 0 1 2-01-01-03-156 DAL-SW-T808-DB-121205 DAL-SW-T808-FMS-121205 DAL-SW-T809-0-121205 ISO12-01-01-03-157 DAL-SW-T810-0-121205 SRS Recovery (%) 13C3-PFBA 13C,,-PFOA 13C4-PFOS 93.2 94.6 94.6 93.0 94.0 96.8 94.2 96.6 93.0 94.4 94.0 924 95.8 96.2 95.0 94.6 95.0 99.4 91.6 952 93.4 94.4 93.6 94.4 94.4 93.8 928 95.8 94.8 926 93.0 932 94.6 95.6 95.0 96.0 85.6 83.2 95.4 84.8 83.8 86.0 86.0 86.0 85.5 88.6 84.6 88.8 88.8 85.6 87.0 86.2 85.2 86.6 926 926 929 82.4 87.8 93.6 86.2 89.0 90.4 87.0 88.6 81.0 87.2 84.8 87.4 91.0 93.6 930 92.9 94.1 93.5 95.4 91.6 927 90.2 91.6 89.3 91.4 929 89.7 93.5 96.2 96.2 97.7 94.6 97.7 91.8 90.0 94.9 100 91.0 933 92 1 91.4 91.4 927 939 91.4 96.2 923 96.2 93.7 92.5 923 PAGE 13 OF 52 3M Environmental Laboratory Report No. IS012-01-01-03 Table 2 continued. Surrogate Recovery Standards Summary SRS Recovery (%) 3M UMS ID Sample Description 13c 3-p f b a Transect Sampling Locations IS 0 1 2-01-01-03-158 IS 0 1 2-01-01-03-159 IS 0 1 2-01-01-03-160 IS 012-01-01-03-161 IS 0 1 2-01-01-03-162 IS 0 1 2-01-01-03-163 IS 0 1 2-01-01-03-164 IS 0 1 2-01-01-03-165 IS 0 1 2-01-01-03-166 IS 0 1 2-01-01-03-167 IS 0 1 2-01-01-03-168 ISO12-01-01-03-169 DAL-SW-T901-0-121205 DAL-SW-T902-0-121205 DAL-SW-T903-0-121205 DAL-SW-T904-0-121205 DAL-SW-T905-0-121205 DAL-SW-T906-0-121205 DAL-SW-T907-0-121205 DAL-SW-T908-0-121205 DAL-SW-T909-0-121205 DAL-SW-T909-DB-121205 DAL-SW-T909-FMS-121205 DAL-SW-T910-0-121205 95.6 93.0 95.8 94.6 952 96.4 98.0 94.0 93.0 94.4 932 94.0 Trip Blank Samples and Equipment Blanks IS012-01-01-03-001 DAL-MSW-LS-EQB01 IS 0 12-01-01-03-002 DAL-MSW-LS-EQB02 IS 0 12-01-01-03-003 DAL-MSW-LS-EQB03 IS 0 12-01-01-03-060 DAL-SW-TRIP01-0-121116 IS 0 12-01-01-03-061 DAL-SW-TRIP02-0-121116 IS012-01-01-03-170 DAL-SW-TRIP-01-0-121203 IS 012-01-01-03-171 DAL-SW-TRIP-01-FMS-121203 ISO12-01-01-03-172 DAL-IPW-LS-EQB04 96.0 90.8 93.6 93.0 92.4 94.4 96.6 932 13c ,,-p f o a 86.0 88.2 89.2 88.0 90.6 83.8 88.4 89.4 86.2 95.6 87.8 95.0 88.0 84.8 86.4 89.4 87.2 85.2 88.6 87.2 13C4-PFOS 94.1 91.8 91 0 90.0 93.7 939 939 95.0 96.9 96.2 95.6 923 95.2 95.0 92.5 90.6 92.5 929 88.1 933 2 Method Summary 2.1 Methods Analysis of the surface w ater sam ples w as com pleted following 3M Environm ental Laboratory method E T S -8 -0 4 4 .1 "M etho d o f A nalysis for th e D eterm ination o f Perfluorinated C o m p o u n d s in W a te r by L C /M S /M S ". T a b le 2 lists th e target an alytes fo r this sam pling event. Table 3. Target Analytes. Targ et A n alytes Perfluorobutanoic Acid (C4 Add) Perfluorohexanoic Add (C6 Acid) Perfluoroheptanoic Add (C7 Add) Periluorooctanoic Add (C8 Add) Perfluorobutanesutfonate (C4 Sulfonate) Perfluorohexanesulfonate (C6 Sulfonate) Perfluorooctanesutfonate (C8 Sulfonate) Periluorobutanesulfonamide Periluorooctanesulfonamide 2-(N-methyl-PFOSA) acetic acid 2-(N-ethyl-PFOSA) acetic acid A cronym PFBA PFHxA PFHpA PFOA PFBS PFHS PFOS FBSA FOSA MeFOSAA EtFOSAA R eferen ce M aterial Structure Linear Linear Linear Linear + Branched Linear Linear Linear + Branched Linear Linear + Branched Linear Linear PAGE 14 OF 52 3M Environmental Laboratory Report No. IS012-01-01-03 2.2 Sample Collection W a te r sam p les w e re collected in 2 5 0 m L N a lg e n e TM (high-density polyethylene) bottles p rep ared a t the 3M Environm ental Laboratory. Laboratory prepared Trip Blanks and Trip Blank field m atrix spikes w ere sent with the set of collection bottles. S am p les w ere returned to th e 3M Environm ental Laboratory on N ovem ber 6, 30 and D ecem b er 11, 2 0 1 2 , at am bient conditions. S am ples w ere stored refrigerated at the laboratory after receipt. T able 3 defines how the sam ples w ere identified. Table 4. Sam ple Description Key Code. String Number Exam ple 1 1 2 3 4 5 6 Exam ple 2 1 2 3 4 5 6 String Descriptor Example D A L-S W -R 01L1-0-121116 (Fish Sam pling Location) Sample Location DAL= Decatur, Alabama Sample Type SW = Surface Water Fish Sampling Reach R01 = Reach 1 Sampling Location L1 = Location 1 (three locations sampled within each fish sampling reach) Sample Type 0 = primary sample DB = duplicate sample FMS = Field Matrix Spike Sampling Date 121116-November 16,2012 DA L-SW -T805-0-121205 (Transect Sam pling Location) Sample Location DAL= Decatur, Alabama Sample Type SW = Surface Water Transect Sampling Location T8=Transect8 Sampling Location Sample Type 05 = Location 5 (ten locations sampled within each transect ) 0 = primary sample DB = duplicate sample FMS = Field Matrix Spike Sampling Date 121205- December 05,2012 i i | | ! PAGE 15 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 T a b le 5 sum m arizes the general location of the fifteen fish sampling reaches and nine transect locations Table 5. Sam pling Locations. Fish Sampling reaches R01 Tennessee River RM 320 below Triana R02 Point Mallard RM 307.5 R03 Tennessee River RM 307.5 R04 Betty Rye Branch and Dry Branch RM 303 R05 Bakers Creek Mouth RM 301 R06 Swan Creek Mouth RM 301 R)7 Tennessee River RM 298 R08 Round Island Creek Mouth RM 298 R09 Fox Creek Mouth RM 296 R10 Browns Ferry - RM 294 R11 Mallard Creek Mouth RM 293.5 R12 Elk River Mouth RM 285 R13 Tennessee River RM 281 R14 Tennessee River RM 273 R15 Tennessee River RM 264 Transect Sampling Locations T1 Below Triana coincides with fish reach RM 320 T2 Coincides with fish samplinq at RM 307.5 T3 Coincides with fish samplinq reach at RM 303 T4 Coincides with fish samplinq reach at RM 301 T5 Coincides with fish samplinq reach at RM 298 T6 Coincides with fish samplinq reach at RM 296 T7 Coincides with fish samplinq reach at RM 293.5 T8 Coincides with fish sampling reach at RM 281 T9 Coincides with fish sampling reach at RM 264 2.3 Sample Preparation All sam p le s w e re initially p rep ared by rem oving a 0 .4 m L aliquot o f th e w ell m ixed s a m p le an d diluting it with 0 .4 m L of m ethanol (dilution factor of 2). During the preparation of the laboratory control sam ples, an aliquot of a separate internal standard spiking solution w a s added to the laboratory control sam ples (nom inal concentration of 2 0 0 or 100 ng/L) T h e sam ple bottles w e re spiked with an internal standard mix at a nom inal concentration of 100 ng/L prior to being sent to the field for sam ple collection. T h e laboratory control sam ples w e re then diluted w ith m ethanol in th e s a m e m a n n e r as th e sam p les. All sam p les w e re prepared for P F B A analysis by rem oving an aliquot of the well m ixed sam ple and placing it in an autovial for analysis. S everal o f th e p rep ared sam p le s w e re re -a n a ly ze d fo r P F O S to achieve a lower limit of quantitation than w as obtainable by the analysis of the sam ples diluted with m eth an o l. During the preparation of the laboratory control sam ples, an aliquot of a separate internal standard spiking solution w a s added to the laboratory control sam ples (nom inal concentration o f 100 ng/L). T h e PAGE 16 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 sam ple bottles w ere spiked with an internal standard mix at a nominal concentration of 100 ng/L prior to being sent to the field for sam ple collection. 2.4 Analysis All sam p les and quality control sam p les w e re an alyzed for eleven target analytes using high perform ance liquid chrom atography/tandem m ass spectrom etry (H P L C /M S /M S ). Pertinent instrum ent p aram eters, the liquid chrom atography gradient program , and th e specific m ass transitions an alyzed are described in th e tab les below . T h e following analytical runs w ere processed and reported herein. 1 2 /2 8 /1 2 A nalysis: S a m p le s w e re an aly ze d for all a n alytes by direct injection solvent dilution, h o w ever the Q C criterias w as not m et for several analytes and the L O Q w as higher than anticipated for P F H S an d P F O S . S in ce no im p ro vem en t w a s s een in th e L O Q for P F H S an d P F O S from th e re-analysis, sam ple results from this run w ere reported for P F H S , P F O S , FB SA , F O S A , M e F O S A A , E tF O S A A , and P F O S -S u rro g a te . 1 /5 /1 3 Analysis: S am ples w e re re-analyzed by direct injection solvent dilution for P FH xA , P FH pA , P F O A , P F B S , P F H S , an d P F O S . S in ce no im p ro vem en t w a s seen in the L O Q for P F H S and P F O S , s a m p le results w e re reported fo r P F H S an d P F O S from th e initial analysis. D u e to high background, P F B S w as not reported from this run. S am p le results from this run w ere reported for P F H xA P FH p A P FO A and PFOA-Surrogate. 1 /1 1 /1 3 Analysis: S am p les w e re re-analyzed by direct injection solvent dilution and reported for P FB S . 1 /2 8 /1 2 Analysis: This run w a s a re-analysis by direct injection solvent dilution o f locations D A L S W R 0 5 L 1 , D A L S W R 0 6 L 2 , D A L S W T 4 0 1 , and D A L S W T 7 0 7 , to verify results from initial analysis. F B S A results w ere reported for D A L S W R 0 5 L 1 , P FB S , FBSA , FO S A , M eF O S A A , and E tF O S A A w ere reported for D A L S W R 0 6 L 2 , an d P F B S , P F O S , F B S A , F O S A , an d 13C 4-P F O S w e re reported for D A L SW T707. 1 /3 1 /1 3 Analysis: This run w a s a re-analysis by direct injection solvent dilution o f locations D A L S W R 0 6 L 2 an d D A L S W T 7 0 7 to verify results from initial analysis. P F H x A , P F H p A , P F O A , an d 13C 4P FO A w ere reported for DAL S W R 06L2 and DAL S W T 707. 2 /1 /1 3 Analysis: T h is run w a s a re-analysis o f all sam pling locations that had a P F O S b elo w th e limit of quantitation for th e initial sa m p le analysis. T his w a s a direct injection (no solvent dilution) analysis. Sam pling locations D A L S W R 0 6 L 1 , D AL S W R 06L2, and D AL S W R 06L3 w ere inadvertently m issed during th e re-analysis an d w e re later a n a ly ze d on 2 /7 /1 3 . S a m p le s w e re reported for P F O S an d 13C 4PFOS. 2 /4 /1 3 Analysis: T h is w a s a direct injection (no solvent dilution) analysis o f all sa m p le s for P F B A and 13C 3-P F B A . 2 /6 /1 3 Analysis: This run w as a re-analysis by direct injection (no solvent dilution) of sampling locations, D A L S W R 03L 1 - L3 an d D A L S W R 0 6 L 1 -L 3 for P F O S an d 13C 4-P F O S . PAGE 17 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 D u e to th e natu re of th e sam p le, th e w id e ran g e o f concentrations found in th e s am p le, and th e en vironm ental occurrence of m ultiple isom ers of th e laboratory's an alytes o f interest, th e softw are used fo r processing the analytical results is not ab le to consistently integrate th e analytical p eak, m an u al integration o f th e analytical p e a k is necessary. All m an u al integrations a re p erform ed following th e procedures outlined in m etho d E T S -1 2 -0 1 0 . T h e consistency o f th e laboratory's integration is ensured through th e training o f laboratory personnel, th e p e e r re v ie w p rocess required for all m an u al integrations, the review of m anual integrations by the Q A U , and w here necessary the review of m anual integrations by laboratory m anagem ent. Table 6. Instrument Parameters. Instrument Name Analysis Dates Analytical Method Liquid Chromatograph Guard column Analytical column Injection Volume Mass Spectrometer Ion Source Electrode Polarity Software ETS Kirk 12/28/12 and 1/28/13 ETS-8-044.1 Agilent 1260 Betasil C 18 (4.6 mm X 100 mm), 5 u Betasil C 18 (4.6 mm X 100 mm), 5p 100 uL Applied Biosystems API 5500 Turbo Spray Turbo ion electrode Negative Analyst 1.5.2 ETS McCoy 1/5/13,1/11/13,1/31/13, 2/1/13, 2/4/13, and 2/6/13 ETS-8-044.1 Agilent 1290 Prism RP (2.1 mm X 50 mm), 5 u Betasil C 18 (2.1 mm X 100 mm), 5u 100 uL Applied Biosystems API 5500 Turbo Spray Turbo ion electrode Negative Analyst 1.5.2 PAGE 18 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 7. Liquid Chromatography Conditions. Step Num ber 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 T o tal Tim e (m in) F lo w R ate (pL/m in) Percent A (2 m M am m o niu m acetate) Analysis Dates:12/28/12 and 1/28/13 0.00 750 90.0 0.50 750 4.00 750 90.0 70.0 6.00 750 11.0 750 70.0 20.0 13.0 750 20.0 135 750 16.0 750 10.0 10.0 16.5 750 19.0 750 90.0 90.0 Analysis Dates: 1/5/13,1/11/13,1/31/13,2/1/13, and 2/6/13 0.00 300 90.0 0.50 300 90.0 4.00 300 70.0 6.00 300 11.0 300 70.0 20.0 13.0 300 13.5 300 20.0 10.0 16.0 300 10.0 16.5 300 19.0 300 90.0 90.0 Analysis Date 2/4/13 0.00 300 0.50 300 90.0 90.0 4.00 300 70.0 6.00 300 70.0 6.10 300 10.0 8.00 300 8.10 300 10.0 90.0 10.0 300 90.0 Percent B (M eth an o l) 10.0 10.0 30.0 30.0 80.0 80.0 90.0 90.0 10.0 10.0 10.0 10.0 30.0 30.0 80.0 80.0 90.0 90.0 10.0 10.0 10.0 10.0 30.0 30.0 90.0 90.0 10.0 10.0 PAGE 19 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 8. Mass Transitions. A n a ly te Mass Transition Q 1 /Q 3 Internal Standard Mass Transition Q 1/Q 3 PFBA 213/169 F cjpfba 217/172 PFHxA 313/119 313/269 f 3C JP F H xA 315/270 PFHpA 363/319 363/119 f 3C JP F H pA 367/322 413/369 PFOA 413/219 f 3CrfPFOA 421/376 413/169 PFBS 299/80 299/99 fO JP FB S 303/84 PFHS 399/99 399/80 ? 3C J P F H S 402/80 499/99 PFOS 499/80 ? ]CdPFOS 507/80 499/130 FBSA 298/78 F o jpfbs 303/84 FOSA 498/78 [ ,3CfJFOSA 506/78 570/219 573/219 MeFOSAA 570/169 d 3-M eF O S A A 573/169 570/83 573/83 584/219 589/219 EtFOSAA 584/169 d5-EtFOSAA 589/169 584/83 589/83 [ ,3C J P F B A surrogate 216/172 ["C J P F B A 217/172 l 13C J P F O A surrogate 417/372 f 3CnlPFOA 421/376 ^ C J P F O S surrogate 503/80 f 3CglPFOS 507/80 Dwell time was 20 msec for each transition. The individual transitions were summed to produce a "total ion chromatogram" (TIC), which was used for quantitation. | I ! ' ' 3 Analytical Results 3.1 Calibration S am ples w ere analyzed against a m atrix-m atched stable isotope internal standard calibration curve. Calibration standards w e re prepared by spiking known am ounts of stock solutions into 2 5 m L of 50:50 laboratory reagent w ater:m ethanol. T h e calibration standards contained an internal standard mix at a nominal concentration of 50 ng/L. A total of nine calibration standards ranging from 1 ng/L to 2 5 0 ng/L (nom inal) w ere prepared. O f these nine calibration standards, six contained the surrogates at concentrations ranging from 7 .5 ng/L to 2 5 0 ng/L nom inal. A quadratic, 1/x w eighted, calibration curve of th e ratio o f the standard p e a k a re a counts o ver th e internal standard p eak a re a counts w a s used to fit the d ata for each analyte. T h e data w e re not forced through zero during the fitting process. Calculating the standard concentrations using the peak a rea ratios and the resultant calibration curve confirm ed accuracy of each curve point. T h e reference standards of P F O A and P F O S used to prepare the calibration standards consisted of both linear and branched isomers. For sam ples that exceeded the PAGE 20 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 above calibration range, an additional set of standards w ere prepared which extended the calibration range to 2 0 0 0 ng/L. Each curve point w as quantitated using the overall calibration curve and reviewed for accuracy. Method calibration accuracy requirem ents of 1 0 0 2 5 % (1 0 0 3 0 % for the lowest curve point) w ere m et for all analytes. T h e correlation coefficient (r) w a s g re a te r th an 0 .9 9 5 for all analytes. S am ples for P F B A and P F O S w e re analyzed against a m atrix-m atched stable isotope internal standard calibration curve. Calibration standards w ere prepared by spiking known am ounts of stock solutions into 2 5 m l. o f laboratory reag en t w ater. T h e calibration stan d ard s contained an internal standard m ix at a nominal concentration of 100 ng/L. A total of nine calibration standards ranging from 1 ng/L to 2 50 ng/L (nom inal) w ere prepared. O f these nine calibration standards, six contained the surrogates at concentrations ranging from 10 ng/L to 2 5 0 ng/L nom inal. A quadratic, 1/x w eighted, calibration curve o f the ratio o f the standard p eak a re a counts o ver the internal standard p e a k a re a counts w a s used to fit the d ata for each analyte. T h e data w e re not forced through zero during the fitting process. Calculating the standard concentrations using the peak a rea ratios and the resultant calibration curve confirm ed accuracy of each curve point. T h e reference standard of P F O S used to prepare the calibration standards consisted of both linear and branched isomers. Each curve point w a s quantitated using the overall calibration curve and reviewed for accuracy. Method calibration accuracy requirem ents of 1 0 0 2 5 % (1 0 0 3 0 % for the lowest curve point) w ere m et for all analytes. T h e correlation coefficient (r) w a s g reater than 0 .9 9 5 for P F B A , P F O S , 13C 3-P F B A , and 13C 4-P F O S . 3.2 System Suitability A calibration standard w as analyzed four tim es at the beginning of the analytical sequence to dem onstrate overall system suitability. T h e acceptance criteria of less than or equal to 5 % relative standard deviation (R S D ) for p eak a rea ratio and retention tim e criteria of less than or equal to 2 % R S D w e re m e t for all analytes. 3.3 Limit of Quantitation (LOQ) T h e L O Q as defined in m ethod E T S -8 -0 4 4 .1 is th e low est n on-zero calibration stan d ard in th e curve that m eets linearity and accuracy requirem ents and for which the area counts are at least twice those of the appropriate blanks. T h e L O Q s associated with th e s am p le analysis a re listed in th e T a b le 8 below . PAGE 21 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 9. LOQ A n aly te PFHxA Analysis Date 1/5/13 PFHpA PFOA 1/5/13 1/5/13 PFBS PFHS 1/11/13 12/28/12 PFOS 12/28/12 FBSA 12/28/12 FOSA 12/28/12 N-MeFOSAA N-EtFOSAA 12/28/12 12/28/12 PFBS PFOS 1/28/13 1/28/13 FBSA 1/28/13 FOSA 1/28/13 MeFOSAA EtFOSAA 1/28/13 1/28/13 PFHxA PFHpA 1/31/3 1/31/3 PFOA 1/31/3 PFOS 2/1/13 PFBA 2/4/13 PFOS 2/6/13 (1) A dilution factor of 2 was applied to the LOQ. LOQ, ng/L TM 2.00TM 2 .0 0 |1) 1.92TM 5 . 0 0 111 5.00TM 9.28 TM 2.00 TM 2.00 TM 2.00 TM 5.00 TM 5.00 TM 9.28 TM 2.00 TM 2.00 TM 9.98 TM 15.0TM 2.00 TM 2.00 TM 4.80 TM 0 927 5.00 0 927 3.4 Continuing Calibration During the course of each analytical sequence, continuing calibration verification sam ples (C C V s ) w ere a n a ly ze d to confirm that th e instrum ent resp o nse an d th e initial calibration curve w e re still in control. All reported sam ple results w ere bracketed by C C V s that m et m ethod criteria of 1 00% 2 5 % with the following exceptions: 12/2 8 /1 2 Analysis: Tw enty-one (2 1 ) C C V sam ples w ere anlyzed over the course of the analysis. O ne C C V for M e F O S A A did not m e e t m ethod acceptance criteria with a recovery o f 126% . T w o C C V s for E tF O S A A did not m eet m ethod acceptance criteria with recoveries of 1 2 6 % and 1 2 7 % . O n e of the C C V sam p le s w a s a stan d ard th at did not contain th e surrogate 13C 4-P F O S . 1/5/13 Analysis: Tw enty (2 0 ) C C V sam ples w ere anlyzed over the course of the analysis. O n e of the C C V sam p le s w a s a stan d ard th at did not contain th e surrogate 13C 4-P F O A . 2 /1 /1 3 Analysis: Thirteen (13) C C V sam ples w ere analyzed. T w o of the C C V sam ples w as a standard th at did not contain the surrogate 13C 4-P F O S . T w o C C V s o f th e s a m e concentration did not m e e t m ethod acceptance criteria for P F O S with recoveries of 1 28% and 152% , however; the internal standard area counts suggest that the autiovial m a y have been low on sam ple. A new vial of the standard w as analyzed at the end of the run and m et m ethod acceptance criteria. 3.5 Blanks T h ree types of blanks w ere prepared and analyzed with the sam ples: solvent blanks (procedural blanks), field/trip blanks, and equipm ent rinseate blanks for the surface w ater sam ples. Each blank result w a s review ed and used to evaluate m ethod perform ance. Procedural blank results w ere reviewed PAGE 22 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 according to the m ethod and used to evaluate method perform ance to determ ine the L O Q for each analyte. 3.6 Lab Control Spikes (LCSs) Low , m id, an d high lab control spikes w e re p rep ared an d a n a ly ze d in triplicate fo r th e targ et analytes, while only low and high lab control spikes w ere prepared for the surrogates. L C S s w e re prepared by spiking known am ounts o f the analytes into 10 m L o f laboratory reagent w ater. W h e re applicable, the L C S s w e re th en diluted 1:1 with m ethan o l in th e s a m e m a n n e r as th e sam p les. T h e method acceptance criteria states that the average recovery of LC S s be 1 00% 2 0 % with a R SD < 2 0 % , w h en evalu ated indep end en tly a t e ach concentration level. All L C S s m e t th e s e criteria with the following exceptions. 12/2 8 /1 2 Analysis: T w o sets of LC S sam ples w ere prepared and analyzed with the sam ples. O ne set w as prepared on 12/27/12 and the second set w as prepared on 12/28/12. T h e low set of LC S s prepared on 1 2 /2 7 /1 2 w ere below the limit o f quantitation (B L O Q ) for P F O S , 1 3 C 4 -P F O S , and E tF O S A A . A recovery could not be calculated. T w o of the three low LC S s prepared on 12/2 7 /1 2 for P F H S w ere B LO Q . For the one replicate that w as reportable, the recovery w as 148% . T h e low set o f L C S s p rep ared on 1 2 /2 8 /1 2 w e re B L O Q for 13C 4-P F O S . T w o o f the th ree low LC Ss for P F O S and E tF O S A A w ere B LO Q . For the one replicate that w as reportable for P F O S , the recovery w a s 2 6 4 % . This L C S w as shown to be contam inated for P F O S during a re-anlysis of the sam ples on 1/5/13, however, no P F O S results are being reported from the 1 /5 /1 3 analysis. T h is replicate w a s not used in th e determ ination o f th e m etho d uncertainty in Secion 3.7. T h e low set of LCSs for M e F O S A A had a R P D value of 30% . 1/5/13 Analysis: T w o sets of LC S sam ples w ere prepared and anlyzed with the sam ples. O ne set w as prepared on 12/27/12 and the second set w as prepared on 12/28/12. T h e low set of LCSs prepared on 12/2 7 /1 2 had an average recoveries of 7 3 .1 % for PFH pA. 6 4 .3 % for P F O A , an d 1 5 9 % for 13C 4-P F O A . T h e high recovery for the su rro g ate13C 4-P F O A is possibly d u e to th e tra c e level o f 13C 4-P F O A present in th e 13C 8-P F O A internal standard. T h e low set o f L C S s p rep ared on 1 2 /2 8 /1 2 w e re B L O Q for 13C 4-P F O A . 1/11/13 Analysis: T w o sets of LCS sam ples w ere preapred and anlyzed with the sam ples. O ne set w as prepared on 12/27/12 and the second set w as preapred on 12/28/12. Both sets o f low level L C S s had recoveries n ear 2 0 0 % . Further investigation into the high recoveries concluded that the spiking solution that w as used to prepare the low level L C S s w a s contam inated with P FB S . T h e mid and high sets of LC S s m et m ethod acceptance criteria. T h e low level L C S s a re reported herein, but w e re not in th e determ ination o f th e m ethod uncertainty in Secion 3 .7 . 1/28/13 Analysis: Four spike levels w ere prepared for the target analytes, with only two of the spike levels contained the surrogates. T h e low set of LC S s (5 ppt) prepared on 1 /2 8 /1 3 w ere B LO Q for M e F O S A A and E tFO SA A . T h e low-mid set of LCSs (10 ppt) prepared on 1/28/13 w ere B LO Q for EtFO SA A . O n e of the three low-mid set of LCSs w as B LO Q for M eF O S A A . This spike level w a s at the L O Q and had an a verag e recovery of 127% . PAGE 23 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 1/31 /1 3 Analysis: Four spike level w ere prepared for the target analytes, with ony tw o of the spike levels contained the surrogates. T h e low set of LC S s (5 ppt) prepared on 1/28 /1 3 w ere B LO Q for PFO A. A m etho d deviation is included with th e ra w data. T h e L C S results w e re used to d eterm in e overall m ethod uncertainty in Section 3 .7 . T h e following calculations w e re used to g e n e ra te d ata in T a b le 10 for laboratory control spikes. Calculated Concentration LCS Percent Recovery = 100% Spike Concentration LCS% RSD = standard deviation LCS replicates 100% average LCS recovery PAGE 24 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 12/28/12 Lab ID LCS-121227-1 LCS-121227-2 LCS-121227-3 Average %RSD LCS-121227-4 L C S -121227-5 L C S -121227-6 Average %RSD LCS-121227-7 LCS-121227-8 LCS-121227-9 Average %RSD PFHS Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.00 <5.00 5.00 5.00 <5.00 7.41 1 4 8 % 1(2) 49.8 49.8 48.9 50.5 49.8 47.9 99.0 99.0 99.0 98.4% 2.5% 94.5 100 99.0 98.8% 3.0% %Recovery N A '1' N A 111 148 98.2 101 96.1 95.5 101 100 PFOS (Linear + Branched) Spiked Concentration (ng/L) Calculated Concentration (ng/L) %Recovery 4.63 <9 29 N A '1' 4.63 4.63 <9 28 <9.28 N A |1> N A <1) NA 46.1 41.9 91.0 46.1 42.2 91.5 46.1 44.8 97.2 93.2% 3.7% 91.8 93.8 102 91.8 89.9 98.0 91.8 88.9 96.9 99.0% 2.7% ETS-8-044.1 Analyzed 12/28/12 Lab ID LCS-121227-1 LCS-121227-2 LCS-121227-3 Average %RSD LCS-121227-4 LCS-121227-5 LCS-121227-6 Average %RSD LCS-121227-7 LCS-121227-8 L C S -121227-9 Average %RSD FBSA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.00 5.00 5.00 49.8 49.8 49.8 4.81 5.39 5.12 102% 5.8% 46.8 49.7 52.0 99.0 99.0 99.0 99.3% 5.1% 94.6 97.4 100 98.3% 2.7% %Recovery 96.1 108 102 94.0 99.9 104 95.6 98.4 101 FOSA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.00 5.00 5.00 4.46 5.08 4.30 92.4% 9.1% 49.8 49.8 49.8 99.0 99.0 99.0 50.7 47.5 49.9 99.1% 3.5% 99.6 100 103 102% 1.7% %Recovery 89.3 102 86.0 102 95.3 100 101 101 104 (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 25 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 12/28/12 Lab ID LCS-121227-1 LCS-121227-2 LCS-121227-3 Average %RSD LCS-121227-4 LCS-121227-5 LCS-121227-6 Average + %RSD LCS-121227-7 LCS-121227-8 LCS-121227-9 Average %RSD MeFOSAA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 4.99 4.14 4.99 4.99 5.26 5.64 49.7 49.7 49.7 100% 16% 49.8 42.3 55.7 98.8 98.8 98.8 99.0% 14% 100 107 106 106% 4.1% %Recovery 82.9 105 113 100 85.1 112 101 109 108 EtFOSAA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 4.99 <5.00 4.99 4.99 <5.00 <5.00 49.7 49.7 49.7 NA 46.6 58.8 48.3 98.8 98.8 98.8 103% 13% 94.8 100 101 100% 3.6% %Recovery N A 11' N A '12' N A '11 93.8 118 97.3 96.0 101 103 ETS-8-044.1 Analyzed 12/28/12 Lab ID LCS-121227-1 LCS-121227-2 LCS-121227-3 Average %RSD LCS-121227-4 LCS-121227-5 LCS-121227-6 Average %RSD LCS-121227-7 LCS-121227-8 LCS-121227-9 Average %RSD 13C4-PFOS Spiked Concentration (ng/L) Calculated Concentration (ng/L) 9.55 9.55 9.55 <14.4 <14.4 <14.4 47.6 47.6 47.6 NA 46.0 50.5 48.8 94.7 94.7 94.7 102% 4.6% 89.1 92.7 94.7 97.3% 3.1% %Recovery NA<1' N A '1' N A '1' 96.6 106 102 94.1 979 100 (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 26 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 12/28/12 Lab ID LCS-121228-1 LCS-121228-2 LCS-121228-3 Average + %RSD LCS-121228-4 LCS-121228-5 LCS-121228-6 Average %RSD LCS-121228-7 LCS-121228-8 LCS-121228-9 Average + %RSD PFHS Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.02 6.78 502 5.02 50.0 50.0 50.0 99.5 99.5 99.5 5.23 5.25 115 15% 52.2 51.7 51.7 103% 0.56% 98.5 106 98.1 102% 4.7% %Recovery 135 104 105 104 103 103 99.0 107 98.6 PFOS (Linear + Branched) Spiked Concentration (ng/L) Calculated Concentration (ng/L) %Recovery 4.65 4.65 4.65 12.3 <9 28 <9.28 264 1(2) N A 11' N A '11 46.4 46.4 46.4 922 922 922 NA 44.0 43.6 46.4 96.3% 3.4% 85.1 96.2 96.3 100% 6.7% 94.8 94.0 100 923 104 104 ETS-8-044.1 Analyzed 12/28/12 Lab ID LCS-121228-1 LCS-121228-2 LCS-121228-3 Average %RSD LCS-121228-4 LCS-121228-5 LCS-121228-6 Average %RSD LCS-121228-7 LCS-121228-8 LCS-121228-9 Average %RSD FBSA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.02 502 5.02 50.0 50.0 50.0 99.5 99.5 99.5 5.28 5.19 5.05 103% 1.9% 47.6 53.6 48.0 99.4% 6.6% 96.9 106 97.2 101% 5.4% %Recovery 105 103 101 952 107 96.0 97.4 107 97.7 FOSA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.02 5.59 5.02 4.86 502 5.39 105% 6.9% 50.0 50.9 50.0 51.6 50.0 50.9 102% 0.56% 99.5 104 99.5 105 99.5 102 104% 1.7% %Recoverv 111 96.9 107 102 103 102 105 105 102 (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 27 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 12/28/12 Lab ID LCS-121228-1 LCS-121228-2 LCS-121228-3 Average %RSD LCS-121228-4 LCS-121228-5 LCS-121228-6 Average + %RSD LCS-121228-7 LCS-121228-8 LCS-121228-9 Average %RSD MeFOSAA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.01 6.99 5.01 6.40 5.01 3.79 49.9 49.9 49.9 1 1 5 % 3 0 % 1(2) 48.1 50.4 52.5 99.3 99.3 99.3 101% 4.3% 101 104 107 104% 3.4% %Recovery 140 128 757 96.3 101 105 101 104 108 EtFOSAA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.01 5.28 5.01 <5.00 5.01 <5.00 49.9 49.9 49.9 105% 48.0 49.6 54.7 99.3 99.3 99.3 102% 7.1% 96.7 122 117 112% 12% %Recovery 105 N A '1' N A '11 96.2 99.5 110 97.4 122 118 ETS-8-044.1 Analyzed 12/28/12 Lab ID LCS-121228-1 LCS-121228-2 LCS-121228-3 Average %RSD LCS-121228-4 LCS-121228-5 LCS-121228-6 Average %RSD LCS-121228-7 LCS-121228-8 LCS-121228-9 Average %RSD 13C4-PFOS Spiked Concentration (ng/L) Calculated Concentration (ng/L) 9.60 9.60 9.60 <14.4 <14.4 <14.4 47.8 47.8 47.8 NA 47.2 48.7 48.9 101% 1.9% 95.1 87.4 95.1 90.9 95.1 90.4 94.2% 2.1% %Recovery NA<1' NA<1' NA<1' 98.7 102 102 91.9 95.6 95 1 (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 28 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 1/5/13 Lab ID LCS-121227-1 LCS-121227-2 LCS-121227-3 Average %RSD LCS-121227-4 LCS-121227-5 LCS-121227-6 Average + %RSD LCS-121227-7 LCS-121227-8 LCS-121227-9 Average %RSD PFHxA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.00 4.76 5.00 5.00 4.77 4.89 49.8 49.8 49.8 96.1% 1.5% 48.1 51.5 50.5 99.0 99.0 99.0 100% 3.3% 102 102 101 103% 0.56% %Recovery 95.2 95.4 97.8 96.6 103 101 103 103 102 PFHpA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.00 4.35 5.00 5.00 49.8 49.8 49.8 3.04 3.57 73.1% 1 8 % 1(2) 45.6 47.8 52.8 99.0 99.0 99.0 97.8% 7.6% 925 106 102 101% 6.9% %Recovery 87.0 60.9 71.3 91.5 96.0 106 93.4 107 103 ETS-8-044.1 Analyzed 1/5/13 Lab ID LCS-121227-1 LCS-121227-2 LCS-121227-3 Average %RSD LCS-121227-4 LCS-121227-5 LCS-121227-6 Average %RSD LCS-121227-7 LCS-121227-8 LCS-121227-9 Average %RSD PFOA (Linear + Branched) Spiked Concentration (ng/L) Calculated Concentration (ng/L) %Recovery 4.79 4.79 4.79 2.97 3.50 2.78 62.0 73.1 57.9 47.7 47.7 47.7 64.3% 12% ,2) 425 42.1 42.9 89.0 88.2 89.9 94.9 94.9 94.9 89.0% 0.96% 86.5 90.9 90.6 91.2 95.8 95.5 94.2% 2.7% 13C4-PFOA Spiked Concentration (ng/L) Calculated Concentration (ng/L) %Recovery 10.0 16.9 169 10.0 15.0 151 10.0 15 6 157 159% 5 .8 % (2> 49.7 49.7 49.7 98.8 98.8 98.8 46.3 47.2 47.4 94.5% 1.3% 89.1 85.6 90.2 89.4% 2.7% 93.1 95.0 95.5 90.1 86.7 91.3 (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 29 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 1/5/13 Lab ID LCS-121228-1 LCS-121228-2 LCS-121228-3 Average %RSD LCS-121228-4 LCS-121228-5 LCS-121228-6 Average %RSD LCS-121228-7 LCS-121228-8 LCS-121228-9 Average %RSD ETS-8-044.1 Analyzed 1/5/13 Lab ID LCS-121228-1 LCS-121228-2 LCS-121228-3 Average + %RSD LCS-121228-4 LCS-121228-5 LCS-121228-6 Average %RSD LCS-121228-7 LCS-121228-8 LCS-121228-9 Average + %RSD PFHxA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.02 6.38 5 0 2 6.75 5 0 2 5.01 50.0 50.0 50.0 120% 15% 55.3 54.3 54.9 99.5 99.5 99.5 110% 0.91% 110 106 99.5 106% 4.9% %Recovery 127 134 99.8 111 109 110 110 107 100 PFHpA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.02 4.72 5.02 4.42 5.02 4.78 50.0 50.0 50.0 92.5% 4.1% 46.4 50.6 51.7 99.5 99.5 99.5 98.9% 5.5% 103 108 95.2 103% 6.2% %Recovery 94.0 88.1 95.3 927 101 103 104 108 95.6 PFOA (Linear + Branched) Spiked Concentration (ng/L) Calculated Concentration (ng/L) %Recovery 4.81 4.98 104 4.81 4.09 85.0 4.81 4.55 94.5 47.9 47.9 47.9 94.5% 10% 43.9 46.5 47.0 91.6 972 98.1 95.3 95.3 95.3 95.6% 03.7% 92.1 99.2 90.7 96.7 104 95.1 98.6% 4.8% 13C4-PFOA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 10.0 <15.0 10.0 <15.0 10.0 <15.0 NA 49.9 49.9 49.9 46.1 47.4 46.4 99.3 99.3 99.3 93.4% 1.4% 86.9 85.0 86.4 86.7% 1.1% %Recovery N A '12' N A '1' N A 11' 923 94.9 93.0 87.5 85.6 87.0 (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 30 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 1/11/13 Lab ID LCS-121227-1 LCS-121227-2 LCS-121227-3 Average %RSD LCS-121227-4 LCS-121227-5 LCS-121227-6 Average %RSD LCS-121227-7 LCS-121227-8 LCS-121227-9 Average %RSD PFBS Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.00 10.7 5.00 5.00 10.3 10.2 49.8 49.8 49.8 208% 2.8% ,2) 47.5 50.0 51.1 99.0 99.0 99.0 99.5% 3.8% 97.0 99.9 98.2 99.4% 1.5% %Recovery 215 205 205 95.5 100 103 98.0 101 99.2 ETS-8-044.1 Analyzed 1/11/13 Lab ID LCS-121228-1 LCS-121228-2 LCS-121228-3 Average %RSD LCS-121228-4 LCS-121228-5 LCS-121228-6 Average %RSD LCS-121228-7 LCS-121228-8 LCS-121228-9 Average %RSD PFBS Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.02 502 5.02 9.77 10.3 9.88 50.0 50.0 50.0 199% 2.7% (2) 48.9 50.4 49.9 99.5 99.5 99.5 99.5% 1.6% 98.6 102 100 101% 1.5% %Recovery 195 205 197 97.8 101 99.8 99.1 102 101 (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 31 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 1/28/13 Lab ID LCS-130130-1 LCS-130130-2 LCS-130130-3 Average %RSD LCS-130128-7 LCS-130128-8 LCS-130128-9 Average %RSD LCS-130128-10 LCS-130128-11 LCS-130128-12 Average + %RSD PFBS Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.00 5.12 5.00 5.00 5.44 4.70 49.8 49.8 49.8 102% 7.4% 52.2 53.1 52.9 106% 0.94% 503 489 503 508 503 537 102% 4.8% %Recovery 102 109 93.9 105 107 106 97.3 101 107 ETS-8-044.1 Analyzed 1/28/13 Lab ID LCS-130128-1 LCS-130128-2 LCS-130128-3 Average %RSD LCS-130128-4 LCS-130128-5 LCS-130128-6 Average %RSD LCS-130128-7 LCS-130128-8 LCS-130128-9 Average %RSD LCS-130128-10 LCS-130128-11 LCS-130128-12 Average %RSD PFOS (Linear + Branched) Spiked Concentration (ng/L) Calculated Concentration (ng/L) %Recovery 4.61 3.99 86.6 4.61 3.39 73.5 4.61 4 2 2 91.5 83.9% 1 1 % 921 9.91 108 921 9.59 104 9.21 9.13 99.1 104% 4.3% 46.1 45.7 99.2 46.1 48.6 105 46.1 50.7 110 105% 5.2% 466 474 102 466 494 106 466 519 111 106% 4.2% 13C4-PFOS Spiked Concentration (ng/L) Calculated Concentration (ng/L) NA NA NA NA NA NA 19.0 19.0 19.0 NA 19.8 18.8 20.3 103% 4.0% 95.1 86.5 95.1 87.6 95.1 89.9 92.5% 2.0% NA NA NA NA NA NA NA %Recovery NA NA NA 104 98.9 107 90.9 92 1 94.6 NA NA NA (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 32 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 1/28/13 Lab ID LCS-130128-1 LCS-130128-2 LCS-130128-3 Average %RSD LCS-130128-4 LCS-130128-5 LCS-130128-6 Average + %RSD LCS-130128-7 LCS-130128-8 LCS-130128-9 Average %RSD LCS-130128-10 LCS-130128-11 LCS-130128-12 Average %RSD FBSA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 4.98 4.76 4.98 4.98 9.93 9.93 9.93 49.8 49.8 49.8 503 503 503 4.90 4.69 95.7% 6.4% 9.99 9.07 9.87 103% 2.0% 50.6 54.2 53.0 104% 4.2% 508 530 541 106% 5.2% %Recovery 95.5 89.7 102 101 105.0 104 102 101 109 101 106 112 FOSA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 4.98 5.11 4.98 4.98 9.93 9.93 9.93 49.8 49.8 49.8 503 503 503 4.47 5.10 98.2% 7.5% 10.3 10.5 10.3 104% 0.55% 525 50.1 54.4 105% 3.8% 498 535 561 106% 6.2% %Recovery 103 89.7 102 104 105 104 105 101 109 99.0 106 112 ETS-8-044.1 Analyzed 1/28/13 Lab ID LCS-130128-1 LCS-130128-2 LCS-130128-3 Average %RSD LCS-130128-4 LCS-130128-5 LCS-130128-6 Average %RPD LCS-130128-7 LCS-130128-8 LCS-130128-9 Average %RSD LCS-130128-10 LCS-130128-11 LCS-130128-12 Average %RSD MeFOSAA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 4.97 4.97 4.97 9.91 9.91 9.91 49.7 49.7 49.7 502 502 502 <9.98 <9.98 <9.98 NA 12.0 <9.98 13.1 127% 8.8% 45.7 54.3 50.6 101% 8.5% 645 533 610 119% 9.6% %Recovery N A '12' N A '1' N A 111 121 NA<1' 132 92.0 109 102 128 106 122 EtFOSAA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 4.97 <15.0 4.97 4.97 9.91 9.91 9.91 49.7 49.7 49.7 <15.0 <15.0 NA <15.0 <15.0 <15.0 NA 45.6 50.7 44.3 94.3% 7.2% 502 527 502 575 502 563 111% 4.6% %Recovery NA1' NA1' N A 11' NA11 N A '1' NA11 91.7 102 89.2 105 115 112 (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria o f l 00 20% or a RSD of <20%. PAGE 33 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 1/31/13 Lab ID LCS-130128-1 LCS-130128-2 LCS-130128-3 Average %RSD LCS-130128-4 LCS-130128-5 LCS-130128-6 Average %RSD LCS-130128-7 LCS-130128-8 LCS-130128-9 Average %RSD LCS-130128-10 LCS-130128-11 LCS-130128-12 Average %RSD PFHxA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 4.98 4.01 4.98 4.98 4.97 4.46 9.93 9.93 9.93 89.9% 1 1 % 8.14 9.77 8.76 49.8 49.8 49.8 89.5% 9.2% 46.9 52 1 49.2 99.3% 5.5% 503 547 503 518 503 555 107% 3.5% %Recovery 80.5 99.8 89.5 82.0 98.4 88.2 94.2 105 98.7 109 103 110 PFHpA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 4.98 4.71 4.98 4.98 5.71 5.32 9.93 9.93 9.93 106% 9.8% 10.7 9.20 10.5 49.8 49.8 49.8 102% 8.0% 44.8 49.9 48.4 95.7% 5.4% 503 504 503 575 503 569 109% 7.2% %Recovery 94.5 115 107 108 927 105 90.0 100 97.2 100 114 113 ETS-8-044.1 Analyzed 1/31/13 Lab ID LCS-130128-1 LCS-130128-2 LCS-130128-3 Average %RSD LCS-130128-4 LCS-130128-5 LCS-130128-6 Average %RPD LCS-130128-7 LCS-130128-8 LCS-130128-9 Average %RSD LCS-130128-10 LCS-130128-11 LCS-130128-12 Average %RSD PFOA (Linear + Branched) Spiked Concentration (ng/L) Calculated Concentration (ng/L) %Recovery 4.77 4.77 4.77 9.51 9.51 9.51 47.7 47.7 47.7 <4.80 <4.80 <4.80 NA 9.62 9.64 9.07 99.1% 3.3% 46.2 49.1 48.2 N A '12' N A ,1> N A ,1) 101 101 95.3 96.9 103 101 100% 3.1% 481 489 102 481 516 107 481 516 107 105% 2.7% 13C4-PFOA Spiked Concentration (ng/L) Calculated Concentration (ng/L) NA NA NA NA NA NA 19.8 19.8 19.8 99.3 99.3 99.3 NA 19.7 21.8 21.1 106% 5.0% 91.1 94.3 94.1 93.8% 2.0% NA NA NA NA NA NA NA %Recovery NA NA NA 99.7 110 107 91.7 95.0 94.8 NA NA NA (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 34 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 10 continued. Laboratory Control Spike Recovery. ETS-8-044.1 Analyzed 2/1/13 Lab ID LCS-130201-1 LCS-130201-2 LCS-130201-3 Average %RSD LCS-130201-4 LCS-130201-5 LCS-130201-6 Average + %RSD LCS-130201-7 LCS-130201-8 LCS-130201-9 Average %RSD LCS-130201-10 LCS-130201-11 LCS-130201-12 Average %RSD PFOS (Linear + Branched) Spiked Concentration (ng/L) Calculated Concentration (ng/L) %Recovery 4.64 4 38 94.5 4.64 4.64 9.30 9.30 9.30 4.63 472 98.8% 3.9% 8.46 8.47 8.91 99.8 102 90.9 91.1 95.8 46.4 46.4 46.4 92.6% 3.0% 44.3 45.7 46.5 95.4 98.5 100 922 922 92.2 98.0% 2.4% 90.0 91.7 90.4 98.4% 1.0% 97.6 99.5 98.0 13C,-PFOS Spiked Concentration (ng/L) Calculated Concentration (ng/L) NA NA NA NA NA NA 19.2 19.2 19.2 NA NA NA NA 18.6 19.5 202 101% 4.2% NA NA NA NA 95.1 93.8 95.1 95.2 95.1 97.0 100% 1.7% %Recovery NA NA NA 96.6 101 105 NA NA NA 98.7 100 102 ETS-8-044.1 Analyzed 2/4/13 Lab ID LCS-130201-1 LCS-130201-2 LCS-130201-3 Average %RSD LCS-130201-4 LCS-130201-5 LCS-130201-6 Average + %RSD LCS-130201-7 LCS-130201-8 LCS-130201-9 Average %RSD LCS-130201-10 LCS-130201-11 LCS-130201-12 Average + %RSD PFBA Spiked Concentration (ng/L) Calculated Concentration (ng/L) 5.00 4.08 5.00 5.00 5.22 5.07 10.0 10.0 10.0 95.6% 13% 9.99 8.72 9.17 50.0 50.0 50.0 92.9% 6.9% 48.9 47.9 47.1 99.5 99.5 99.5 96.0% 1.8% 100 100 99.7 100% 0.58% %Recovery 81.7 104 101 99.9 87.2 91.7 97.8 95.8 94.3 101 100 100 13C3-PFBA Spiked Concentration (ng/L) Calculated Concentration (ng/L) NA NA NA NA NA NA NA 20.0 19.8 20.0 20.2 20.0 21.0 102% 3.0% NA NA NA NA NA NA NA 99.1 98.2 99.1 99.4 99.1 103 101% 2.6% %Recovery NA NA NA 98.9 101 105 NA NA NA 99.1 100 104 (1) NA = Not Applicable; the sample result was below the limit of quantitation and a recovery could not be calculated. (2) The average recovery did not meet method acceptance criteria of 100 20% or a RSD of <20%. PAGE 35 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 3.7 Analytical Method Uncertainty Analytical uncertainty is b ased on historical Q C d a ta th at is control charted an d used to e valu ate m ethod accu racy an d precision. T h e m ethod uncertainty is calculated following E T S -1 2 -0 1 2 .2 . T h e standard deviation is calculated for th e set of a ccu racy results (in % ) obtain ed fo r th e Q C sam p les. For m ethod E T S -8 -0 4 4 .1 , th e m ost recent fifty Q C sam p les w e re used (w here applicable). T h e analytical m ethod uncertainty is calculated by multiplying th e standard deviation by a factor o f 2, which corresponds to a confidence level of 95% . Table 11. Analytical Method Uncertainty Analyte PFBA PFHxA PFHpA PFOA PFBS PFHS PFOS FBSA FOSA MeFOSAA EtFOSAA 13C3-PFBA 13GtPFOA 13C rP F O S Standard Dviation 8.14 10.1 10.6 11.6 10.0 124 8.50 8.38 6.79 12.6 8.47 9.07 8.04 7.88 Method Uncertainty 16% 20% 21% 23% 20% 25% 17% 17% 14% 25% 17% 18% 16% 16% 3.8 Field Matrix Spikes (FMS) A field m atrix spike w a s collected a t 1 0 % o f the sam pling locations to verify th at th e analytical m ethod is applicable to the collected matrix. Field m atrix spikes are generated by adding a m easured volum e of field sam ple to a container spiked by the laboratory with the target analytes prior to shipping sam ple containers for sam ple collection. Field matrix spike recoveries within m ethod acceptance criteria of 1 0 0 3 0 % confirm that "unkn o w n " co m p on en ts in th e sa m p le m atrix do not significantly interfere with the extraction and analysis o f th e an alytes o f interest. T h e referen ce stan d ard s for P F O A an d P F O S in the field m atrix spiking solution consisted of linear and branched isomers. In addition, field m atrix spikes for this project also included the use o f stable isotope surrogate recovery standards (S R S s ) 13C 3-P F B A , 13C 4-P F O A , an d 13C 4-P F O S , which w a s a d d e d at a nom inal concentration o f 5 0 ng/L to all s a m p le bottles prior to s a m p le collection. S urro gate m atrix spike recoveries within m ethod a c c e p tan ce criteria o f 1 0 0 3 0 % confirm that "unknow n" co m p on en ts in th e s a m p le m atrix do not significantly interfere with the preparation and analysis of the analytes of interest. Surrogate recovery standard results a re p resen ted in Table 2 o f th e report. PAGE 36 OF 52 3M Environmental Laboratory Report No. ISO12-01-01-03 Table 12. Field Matrix Spike Level. Description FMS PFBA PFHxA PFHpA 100 100 100 PFOA 95.8 Final Concentration (n g /L )(1> PFBS PFHS PFOS FBSA 100 100 92.7 100 FOSA MeFOSAA EtFOSAA 100 99.8 99.8 (1) Location DAL-SW-T707 was overfilled by 10% (220 mL)_ The FMS true values were adjusted accordingly. ,,_ (Sample Concentration o f F M S -A v era g e Concentration:Field Sample & Field Sample Dup.K FMS Recovery = F--------- ---------------------------------- ----------------------------------------------------- -- t - ------ * i oo% Spike Concentration 4 Data Summary and Discussion T h e tables below sum m arize the sam ple results and field matrix spike (F M S ) recoveries for the sampling locations w here a F M S sam ple w as collected, along with the Trip Blank F M S results. Results and average values are rounded to three significant figures according to E P A rounding rules. B ecause of rounding, valu es m a y v ary slightly from th o se listed in th e ra w d ata. Field m atrix spike an d surrogate recoveries meeting the m ethod acceptance criteria of 30% , dem onstrate that the m ethod(s) w ere appropriate for the given matrix and their respective quantitative ranges. All F M S sam p les m et m ethod acceptance criteria with th e following exception: D A L S W R 05L1; the F M S level w as not appropriate for P FO A , P F O S , and FB S A however, the surrogate recovery standards m et m ethod acceptance criteria. DAL S W R 1 5 L 1 ; the F M S recovery for E tF O S A A w as 6 9 .5 % . W h e n rounded to two significant figures (70% ), the recovery m eets method acceptance criteria of 100% 30% . T h e following sampling locations had a sam ple/sam ple duplciate relative percent difference (R P D ) outside m ethod acceptance criteria o f <20% : DAL S W R02L3; the R P D for PFH xA w as 2 9% and 2 2% for PFHpA. DAL S W T202; the R P D for PFH pA w as 27% . DAL S W T606; the R P D for P FO A w as 25% . PAGE 37 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 13. DAL SW R02L3 3M U M S ID Description IS012-01-01-03-012 DAL-SW-R02L3-0-121116 IS012-01-01-03-013 DAL-SW-R02L3-DUP-121116 IS012-01-01-03-014 DAL-SW-R02L3-FMS-121116 Average Concentration (ng/L) %RPD PFBA C o n c e n tra tio n (n g i) % R ecovery 6.13 NA 6.10 NA 100 93.9 6.12 ng/L 0.49% PFHxA PFHpA Concentration (ngA.) % R eco very 4.83 NA 3.61 NA 111 107 4 .2 2 ng/L 2 9 % lv C o n c e n tra tio n (ng/L) % R eco verv 4.70 NA 3.78 NA 103 98.8 4.24 ng/L 2 2 % (V PFOA Concentration (ngA.) % R eco verv 9 2 8 NA 9 12 NA 101 95.8 9.20 n g /L 1.7% 3M U M S ID Description IS012-01-01-03-012 DAL-SW-R02L3-0-121116 IS012-01-01-03-013 DAL-SW-R02L3-DUP-121116 IS012-01-01-03-014 DAL-SW-R02L3-FMS-121116 Average Concentration (ng/L) %RPD PFBS C o n c e n tra tio n (ngA.) % R eco very <5.00 <5.00 102 NA NA 102 <5.00 ng/L PFHS PFOS Concentration (ngA.) VoRecovery <5.00 <5.00 99.7 NA NA 99.7 <5.00 ng/L C o n c e n tra tio n (ngA.) % R eco very 8.94 8.46 94.7 NA NA 928 8.70 ng/L 5 .5% FBSA Concentration (ngA.) VoRecovery <2.00 <2.00 95.5 NA NA 95.5 <2.00 ng/L 3M U M S ID Description IS012-01-01-03-012 DAL-SW-R02L3-0-121116 IS012-01-01-03-013 DAL-SW-R02L3-DUP-121116 IS012-01-01-03-014 DAL-SW-R02L3-FMS-121116 Average Concentration (ng/L) % RPD/% RSD FOSA C o n c e n tra tio n (ngA.) <2.00 <2.00 101 VoRecovery NA NA 101 <2.00 ng/L MeFOSAA Concentration (ngA.) <2.00 <2.00 95.1 VoRecovery NA NA 95.3 <2.00 ng/L EtFOSAA C o n c e n tra tio n (ngA.) <5.00 <5.00 94.2 VoRecovery NA NA 94.4 <5.00 ngA. NA = Not Applicable (1) The sample / sample duplicate RPD did not meet method acceptance criteria of <20%. PAGE 38 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 14. DAL SW R05L1 3M U M S ID Description IS012-01-01-03-052 DAL-SW-R05L1 -0-121119 IS012-01-01-03-053 DAL-SW-R05L1 -DUP-121119 IS 012-01-01-03-054 DAL-SW-R05L1-FMS-121119 Average Concentration (ng/L) %RPD PFBA C o n c e n tra tio n (ng/L) YoRecovery 58.9 NA 58.3 NA 163 104 58.6 ng/L 1.0% PFHxA PFHpA Concentration (ng/L) YoRecovery 72.5 NA 70.5 NA 176 105 71.5 ng/L 2.8% C o n c e n tra tio n (ng/L) YoRecovery 56.8 59.0 178 NA NA 120 57.9 ng/L 3.8% PFOA Concentration (ng/L) YoRecovery 246 NA 242 NA 353 NC 244 ng/L 1.6% 3M U M S ID Description IS012-01-01-03-052 DAL-SW-R05L1 -0-121119 IS012-01-01-03-053 DAL-SW-R05L1 -DUP-121119 IS 012-01-01-03-054 DAL-SW-R05L1-FMS-121119 A v e ra g e C oncentration (ng/L) YoRPD PFBS C o n c e n tra tio n (ngA.) YoRecovery 124 NA 116 NA 233 113 120 ng/L 6.7% PFHS PFOS Concentration (ngA.) YoRecovery 39.8 NA 42 3 NA 137 96.0 41.1 ng/L 6 .1 % C o n c e n tra tio n (ngA.) YoRecovery 385 NA 405 NA 543 NC 395 ng/L 5.1% FBSA Concentration (ngA.) YoRecovery 1130 NA 1050 NA 1260 NC 1090 ng/L 7.3% 1 I wos. 3M U M S ID Description IS012-01-01-03-052 DAL-SW-R05L1 -0-121119 IS012-01-01-03-053 DAL-SW-R05L1 -DUP-121119 IS 012-01-01-03-054 DAL-SW-R05L1-FMS-121119 A v e ra g e C oncentration (ng/L) YoRPD/YoRSD FOSA C o n c e n tra tio n (noA.) YoRecovery 21.1 18.0 NA NA 116 96.5 19.6 ngA. 16% MeFOSAA Concentration (ngA.) 10.0 NA 8.20 99.0 NA 90.1 9.10 ngA. 2 0 % NA = Not Applicable NC = Not Calculated; the endogenous sample concentration was greater than 2x the spike level. 5? !1 EtFOSAA C o n c e n tra tio n (ngA.) 18.0 NA 17.8 NA 113 95.3 17.9 ng/L 1 .1 % PAGE 39 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 15. DALSW R10L3 3M U M S ID Description IS012-01-01-03-040 DAL-SW-R10L3-0-121119 IS 012-01-01-03-041 DAL-SW- R10L3-DUP-121119 IS012-01-01-03-042 DAL-SW- R10L3-FMS-121119 Average Concentration (ng/L) %RPD PFBA C o n c e n tra tio n (ng/L) YoRecovery <5.00 <5.00 95.9 NA NA 95.9 <5.00 ng/L PFHxA Concentration (ng/L) YoRecovery 2.73 <2.00 102 NA NA 99.3 2.73 ng/L PFHpA C o n c e n tra tio n (ng/L) YoRecovery <2.00 <2.00 94.5 NA NA 94.5 <2.00 ng/L PFOA Concentration (ng/L) YoRecovery 352 3.50 90.1 NA NA 90.4 3.52 ng/L 0.57% 3M U M S ID Description IS012-01-01-03-040 DAL-SW-R10L3-0-121119 IS 012-01-01-03-041 DAL-SW- R10L3-DUP-121119 IS012-01-01-03-042 DAL-SW- R10L3-FMS-121119 A v e ra g e C oncentration (ng/L) YoRPD PFBS C o n c e n tra tio n (ngA.) YoRecovery <5.00 NA <5.00 NA 97.6 97.6 <5.00 ng/L PFHS Concentration (ngA.) YoRecovery <5.00 NA <5.00 NA 91.2 91.2 <5.00 ng/L PFOS C o n c e n tra tio n (ngA.) YoRecovery 6.49 NA 6.63 NA 92.5 927 6.56 ng/L Z 1 % FBSA Concentration (ngA.) YoRecovery <2.00 NA <2.00 NA 94.8 94.8 <2.00 ng/L t 1 wo'- 3M U M S ID Description IS012-01-01-03-040 DAL-SW-R10L3-0-121119 IS 012-01-01-03-041 DAL-SW- R10L3-DUP-121119 IS012-01-01-03-042 DAL-SW- R10L3-FMS-121119 A v e ra g e C oncentration (ng/L) YoRPD/YoRSD FOSA C o n c e n tra tio n (noA.) YoRecovery <2.00 <2.00 99.6 NA NA 99.6 <2.00 ng/L MeFOSAA Concentration (ngA.) <2.00 NA <2.00 NA 91.9 92 1 <2.00 ng/L NA = Not Applicable NC = Not Calculated; the endogenous sample concentration was greater than 2x the spike level. 5? !1 EtFOSAA C o n c e n tra tio n (ngA.) <5.00 <5.00 93.6 NA NA 93.8 <5.00 ng/L PAGE 40 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 16. DALSW R15L1 3M U M S ID Description IS012-01-01-03-024 DAL-SW-R15L1 -0-121117 IS012-01-01-03-025 DAL-SW- R15L1-DUP-121117 IS 012-01-01-03-026 DAL-SW- R15L1 -FMS-121117 Average Concentration (ng/L) %RPD PFBA C o n c e n tra tio n (ng/L) % R ecovery 792 6.62 98.9 NA NA 91.6 7.27 ng/L 18% PFHxA PFHpA Concentration (ng/L) % Recovery 4 2 4 NA 4.71 NA 108 104 4.48 ng/L 1 1 % C o n c e n tra tio n (ng/L) % R eco very 2.34 <2.00 106 NA NA 104 2.34 ng/L PFOA Concentration (ng/L) % R eco very 11.5 NA 109 NA 100 92.6 11.2 ng/L 5.4% 3M U M S ID Description IS012-01-01-03-024 DAL-SW-R15L1 -0-121117 IS012-01-01-03-025 DAL-SW- R15L1-DUP-121117 IS 012-01-01-03-026 DAL-SW- R15L1 -FMS-121117 Average Concentration (ng/L) %RPD PFBS C o n c e n tra tio n (ngA.) % R eco very 14.8 NA 13.7 NA 109 94.8 14.3 ng/L 7 .7% PFHS Concentration (ngA.) VoRecovery <5.00 NA <5.00 NA 95.5 95.5 <5.00 ng/L PFOS C o n c e n tra tio n (ngA.) % R eco very 19.9 NA 18.9 NA 113 101 19.4 ng/L 5 .2% FBSA Concentration (ngA.) % R eco very 34.8 NA 31.8 NA 118 84.7 33.3 ngA. 9 .0% 3M U M S ID Description IS012-01-01-03-024 DAL-SW-R15L1 -0-121117 IS012-01-01-03-025 DAL-SW- R15L1-DUP-121117 IS 012-01-01-03-026 DAL-SW- R15L1 -FMS-121117 Average Concentration (ng/L) % RPD/% RSD FOSA C o n c e n tra tio n (ng/L) % R eco verv <2.00 <2.00 101 NA NA 101 <2.00 ng/L MeFOSAA Concentration (ngA.) <2.00 <2.00 85.9 NA NA 86.1 <2.00 ng/L NA = Not Applicable (1) The field matrix spike recovery did not meet method acceptance criteria of 100 30% S? !1 EtFOSAA C o n c e n tra tio n (ngA.) t I SP <5.00 <5.00 69.4 NA NA 6 9 .5 <1) <5.00 ng/L PAGE 41 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 17. DALSW T101 3M U M S ID Description IS012-01-01-03-062 DAL-SW-T101-0-121203 IS012-01-01-03-063 DAL-SW-T101-DB-121203 IS 012-01-01-03-064 DAL-SW-T101-FMS-121203 Average Concentration (ng/L) %RPD 3M U M S ID Description IS 012-01-01-03-062 DAL-SW-T 101-0-121203 IS 012-01-01-03-063 DAL-SW-T101 -DB-121203 ISQ12-01-01-03-064 DAL-SW-T101-FMS-121203 Average Concentration (ng/L) %RPD PFBA C o n c e n tra tio n (ngA-) % R eco verv <5.00 NA <5.00 NA 94.4 94.4 <5.00 ng/L PFHxA PFHpA Concentration (ngA.) VoRecoverv <2.00 NA <2.00 NA 107 107 <2.00 ng/L C o n c e n tra tio n (ng/L) % R eco verv <2.00 NA <2.00 NA 97.2 97.2 <2.00 ng/L PFOA Concentration (ng/L) VoRecoverv <1 92 NA <1 92 NA 90.0 939 <1.92 ng/L PFBS C o n c e n tra tio n (ng/L) VoRecovery <5.00 <5.00 96.1 NA NA 96.1 <5.00 ng/L PFHS PFOS Concentration (ngn.) VoRecovery <5.00 <5.00 933 NA NA 933 <5.00 ng/L C o n c e n tra tio n (ng/L) VoRecovery 6.13 6.36 91.1 NA NA 91.5 6.25 ng/L 3 .7 % FBSA Concentration (ngA.) VoRecovery <2.00 <2.00 87.7 NA NA 87.7 <2.00 ng/L 3M U M S ID Description IS012-01-01-03-062 DAL-SW-T101-0-121203 IS 012-01-01-03-063 DAL-SW-T101 -DB-121203 IS 0 12-01-01-03-064 DAL-SW-T101-FMS-121203 Average Concentration (ng/L) % RPD/% RSD NA = Not Applicable FOSA C o n c e n tra tio n (ngn.) <2.00 <2.00 98.3 VoRecovery NA NA 98.3 <2.00 ng/L MeFOSAA Concentration (ngn.) <2.00 <2.00 99.9 VoRecovery NA NA 100 <2.00 ng/L EtFOSAA C o n c e n tra tio n (ngn.) <5.00 <5.00 94.7 VoRecovery NA NA 94.9 <5.00 ngA. PAGE 42 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 18. DALSW T202 3M U M S ID Description IS012-01-01-03-075 DAL-SW-T202-0-121203 IS012-01-01-03-076 DAL-SW-T202-DB-121203 IS012-01-01-03-077 DAL-SW-T202-FMS-121203 Average Concentration (ng/L) %RPD 3M U M S ID Description IS012-01-01-03-075 DAL-SW-T202-0-121203 IS012-01-01-03-076 DAL-SW-T202-DB-121203 IS012-01-01-03-077 DAL-SW-T202-FMS-121203 A v e ra g e C oncentration (ng/L) YoRPD PFBA C o n c e n tra tio n (ng/L) YoRecovery 672 5.50 97.3 NA NA 90.6 6.72 ng/L 18% PFHxA PFHpA Concentration (ng/L) YoRecovery 5.16 4.58 103 NA NA 98.1 4.87 ng/L 5.4% C o n c e n tra tio n (ng/L) YoRecovery 4.80 NA 3.64 NA 103 98.8 4 .2 2 ng/L 2 7 % ,1> PFOA Concentration (ng/L) YoRecovery 12.6 107 106 NA NA 98.5 11.7 ng/L 1 6 % PFBS C o n c e n tra tio n (ngA.) YoRecovery <5.00 NA <5.00 NA 99.4 99.4 <5.00 ng/L PFHS PFOS Concentration (ngA.) YoRecovery <5.00 NA <5.00 NA 94.6 94.6 <5.00 ng/L C o n c e n tra tio n (ngA.) YoRecovery 9.93 NA 9.43 NA 88.7 852 9.68 ngA. 5.2% FBSA Concentration (ngA.) YoRecovery <2.00 NA <2.00 NA 91.6 91.6 <2.00 ng/L 3M U M S ID Description IS012-01-01-03-075 DAL-SW-T202-0-121203 IS012-01-01-03-076 DAL-SW-T202-DB-121203 IS012-01-01-03-077 DAL-SW-T202-FMS-121203 A v e ra g e C oncentration (ng/L) YoRPD/YoRSD FOSA C o n c e n tra tio n (noA.) YoRecovery <2.00 <2.00 94.8 NA NA 94.8 <2.00 ng/L MeFOSAA Concentration (ngA.) <2.00 NA <2.00 NA 102 102 <2.00 ng/L NA = Not Applicable (1) The sample / sample duplicate RPD did not meet method acceptance criteria of <20%. S? !1 EtFOSAA C o n c e n tra tio n (ngA.) I oS'P- <5.00 <5.00 96.2 NA NA 96.4 <5.00 ngA. PAGE 43 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 19. DALSW T303 3M U M S ID Description IS 012-01-01-03-088 DAL-SW-T303-0-121203 IS012-01-01-03-089 DAL-SW-T303-DB-121203 IS012-01-01-03-090 DAL-SW-T303-FMS-121203 Average Concentration (ng/L) %RPD 3M U M S ID Description IS 012-01-01-03-088 DAL-SW-T303-0-121203 IS012-01-01-03-089 DAL-SW-T303-DB-121203 IS012-01-01-03-090 DAL-SW-T303-FMS-121203 A v e ra g e C oncentration (ng/L) YoRPD PFBA C o n c e n tra tio n (ng/L) YoRecovery <5.00 <5.00 97 1 NA NA 97.1 <5.00 ng/L PFHxA PFHpA Concentration (ng/L) YoRecovery <2.00 <2.00 106 NA NA 106 <2.00 ng/L C o n c e n tra tio n (ng/L) YoRecovery <2.00 <2.00 97.9 NA NA 97.9 <2.00 ng/L PFOA Concentration (ng/L) YoRecovery 2.78 229 91.3 NA NA 927 2.54 ng/L 19Yo PFBS C o n c e n tra tio n (ngA.) YoRecovery <5.00 NA <5.00 NA 98.0 98.0 <5.00 ng/L PFHS PFOS Concentration (ngA.) YoRecovery <5.00 NA <5.00 NA 94.9 94.9 <5.00 ng/L C o n c e n tra tio n (ngA.) YoRecovery 6.94 NA 751 NA 9 2 9 92.4 7.23 ng/L 7.9Yo FBSA Concentration (ngA.) YoRecovery <2.00 NA <2.00 NA 932 932 <2.00 ng/L t I ONP' 3M U M S ID Description IS012-01-01-03-088 DAL-SW-T303-0-121203 IS012-01-01-03-089 DAL-SW-T303-DB-121203 ISQ12-01-01-03-090 DAL-SW-T303-FMS-121203 A v erag e C oncentration (ngA.) YoRPD/YoRSD NA = Not Applicable FOSA C o n c e n tra tio n (ngA.) YoRecovery <2.00 NA <2.00 NA 100 100 <2.00 ng/L MeFOSAA Concentration (ngA.) <2.00 NA <2.00 NA 102 102 <2.00 ng/L S? !1 EtFOSAA C o n c e n tra tio n (ngA.) <5.00 <5.00 102 NA NA 102 <5.00 ng/L PAGE 44 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 20. DALSW T404 3M U M S ID Description IS 012-01-01-03-101 DAL-SW-T404-0-121203 IS012-01-01-03-102 DAL-SW-T404-DB-121203 IS012-01-01-03-103 DAL-SW-T404-FMS-121203 Average Concentration (ng/L) %RPD 3M U M S ID Description IS 012-01-01-03-101 DAL-SW-T404-0-121203 IS012-01-01-03-102 DAL-SW-T404-DB-121203 IS012-01-01-03-103 DAL-SW-T404-FMS-121203 A v e ra g e C oncentration (ng/L) YoRPD PFBA C o n c e n tra tio n (ng/L) YoRecovery <5.00 <5.00 94.7 NA NA 94.7 <5.00 ng/L PFHxA PFHpA Concentration (ng/L) YoRecovery <2.00 <2.00 106 NA NA 106 <2.00 ng/L C o n c e n tra tio n (ng/L) YoRecovery <2.00 <2.00 102 NA NA 102 <2.00 ng/L PFOA Concentration (ng/L) YoRecovery 225 <1 92 89.9 NA NA 91.5 2.25 ng/L PFBS C o n c e n tra tio n (ngA.) YoRecovery <5.00 NA <5.00 NA 95.0 95.0 <5.00 ng/L PFHS PFOS Concentration (ngA.) YoRecovery <5.00 NA <5.00 NA 94.7 94.7 <5.00 ng/L C o n c e n tra tio n (ngA.) YoRecovery 7 2 9 NA 8.46 NA 9 2 9 91.7 7.88 ng/L 15Yo FBSA Concentration (ngA.) YoRecovery <2.00 NA <2.00 NA 91.0 91.0 <2.00 ng/L t I wO' 3M U M S ID Description IS 012-01-01-03-101 DAL-SW-T404-0-121203 IS012-01-01-03-102 DAL-SW-T404-DB-121203 IS012-01-01-03-103 DAL-SW-T404-FMS-121203 A v erag e C oncentration (ngA.) YoRPD/YoRSD NA = Not Applicable FOSA C o n c e n tra tio n (ngA.) YoRecovery <2.00 NA <2.00 NA 102 102 <2.00 ng/L MeFOSAA Concentration (ngA.) <2.00 NA <2.00 NA 97.4 97.6 <2.00 ng/L S? !1 EtFOSAA C o n c e n tra tio n (ngA.) <5.00 NA <5.00 NA 92.1 9 2 3 <5.00 ng/L PAGE 45 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 21. DALSW T505 3M U M S ID Description IS012-01-01-03-114 DAL-SW-T505-0-121204 IS012-01-01-03-115 DAL-SW-T505-DB-121204 ISQ12-01-01-03-116 DAL-SW-T505-FMS-121204 Average Concentration (ng/L) %RPD 3M U M S ID Description IS012-01-01-03-114 DAL-SW-T505-0-121204 IS012-01-01-03-115 DAL-SW-T505-DB-121204 ISQ12-01-01-03-116 DAL-SW-T505-FMS-121204 A v e ra g e C oncentration (ng/L) YoRPD PFBA C o n c e n tra tio n (ng/L) YoRecovery <5.00 5.48 96.7 NA NA 91.2 5.48 ng/L PFHxA PFHpA Concentration (ng/L) YoRecovery <2.00 <2.00 109 NA NA 109 <2.00 ng/L C o n c e n tra tio n (ng/L) YoRecovery <2.00 <2.00 103 NA NA 103 <2.00 ng/L PFOA Concentration (ng/L) YoRecovery 2 52 3.01 92.8 NA NA 94.0 2.77 ng/L 18Yo PFBS C o n c e n tra tio n (ngA.) YoRecovery <5.00 NA <5.00 NA 98.5 98.5 <5.00 ng/L PFHS PFOS Concentration (ngA.) YoRecovery <5.00 NA <5.00 NA 96.3 96.3 <5.00 ng/L C o n c e n tra tio n (ngA.) YoRecovery 7 2 0 NA 6.78 NA 93.7 93.5 6.99 ng/L 6.0% FBSA Concentration (ngA.) YoRecovery <2.00 NA <2.00 NA 88.4 88.4 <2.00 ng/L 1 1 oS'P- 3M U M S ID Description IS012-01-01-03-114 DAL-SW-T505-0-121204 IS012-01-01-03-115 DAL-SW-T505-DB-121204 ISQ12-01-01-03-116 DAL-SW-T505-FMS-121204 A v erag e C oncentration (ngA.) YoRPD/YoRSD NA = Not Applicable FOSA C o n c e n tra tio n (ngA.) YoRecovery <2.00 <2.00 97.9 NA NA 97.9 <2.00 ng/L MeFOSAA Concentration (ngA.) YoRecovery <2.00 NA <2.00 NA 92.6 92.8 <2.00 ng/L EtFOSAA C o n c e n tra tio n (ngA.) <5.00 <5.00 99.6 NA NA 99.8 <5.00 ng/L PAGE 46 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 22. DALSW T606 3M U M S ID Description IS012-01-01-03-127 DAL-SW-T606-0-121204 IS012-01-01-03-128 DAL-SW-T606-DB-121204 IS012-01-01-03-129 DAL-SW-T606-FMS-121204 Average Concentration (ng/L) %RPD 3M U M S ID Description IS012-01-01-03-127 DAL-SW-T606-0-121204 IS012-01-01-03-128 DAL-SW-T606-DB-121204 IS012-01-01-03-129 DAL-SW-T606-FMS-121204 A v e ra g e C oncentration (ng/L) YoRPD PFBA C o n c e n tra tio n (ng/L) YoRecovery <5.00 <5.00 96.6 NA NA 96.6 <5.00 ng/L PFHxA PFHpA Concentration (ng/L) YoRecovery 2.31 NA 2.41 NA 110 108 2.36 ngA. 4 .2% C o n c e n tra tio n (ng/L) YoRecovery <2.00 <2.00 102 NA NA 102 <2.00 ng/L PFOA Concentration (ng/L) YoRecovery 4.38 3.41 922 NA NA 922 3.90 ng/L 25% n PFBS C o n c e n tra tio n (ngA.) YoRecovery <5.00 NA <5.00 NA 97.9 97.9 <5.00 ng/L PFHS PFOS Concentration (ngA.) YoRecovery <5.00 NA <5.00 NA 97.1 97.1 <5.00 ng/L C o n c e n tra tio n (ngA.) YoRecovery 9 7 2 NA 11.1 NA 96.5 92.9 10.4 ng/L 13Yo FBSA Concentration (ngA.) YoRecovery 2 22 NA 2 18 NA 91.0 88.8 2.20 ngA. 1 .8% 3M U M S ID Description IS012-01-01-03-127 DAL-SW-T606-0-121204 IS012-01-01-03-128 DAL-SW-T606-DB-121204 IS012-01-01-03-129 DAL-SW-T606-FMS-121204 A v erag e C oncentration (ngA.) YoRPD/YoRSD FOSA C o n c e n tra tio n (ngA.) YoRecovery <2.00 <2.00 98.6 NA NA 98.6 <2.00 ng/L MeFOSAA Concentration (ngA.) YoRecovery <2.00 NA <2.00 NA 92.4 92.6 <2.00 ng/L EtFOSAA C o n c e n tra tio n (ngA.) I oS'P- <5.00 NA <5.00 NA 96.5 96.7 <5.00 ng/L NA = Not Applicable (1) The sample / sample duplicate RPD did not meet method acceptance criteria of <20%. PAGE 47 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 23. DALSW T707 3M U M S ID Description IS012-01-01-03-140 DAL-SW-T707-0-121204 IS 012-01-01-03-141 DAL-SW-T707-DB-121204 IS012-01-01-03-142 DAL-SW-T707-FMS-121204 Average Concentration (ng/L) %RPD 3M U M S ID Description IS012-01-01-03-140 DAL-SW-T707-0-121204 IS 012-01-01-03-141 DAL-SW-T707-DB-121204 IS012-01-01-03-142 DAL-SW-T707-FMS-121204 Average Concentration (ng/L) %RPD PFBA C o n c e n tra tio n (ng/L) VoRecovery 8.42 7.66 89.5 NA NA 81.5 8.04 ng/L 9.5% PFHxA PFHpA Concentration (ng/L) %Recovery 4.05 4 53 99.0 NA NA 104 4.29n g /L 11% C o n c e n tra tio n (ng/L) VoRecovery 324 3.77 88.3 NA NA 93.3 3.51 ng/L 15% PFOA Concentration (ng/L) VoRecovery 12.5 11.9 973 NA NA 97.7 12.2 ng/L 4 9 % PFBS C o n c e n tra tio n (ngA.) VoRecovery 19.0 NA 21.0 NA 105 93.5 20.0 ngA. 10% PFHS PFOS Concentration (ngA.) VoRecovery <5.00 NA <5.00 NA 90.0 99.0 <5.00 ng/L C o n c e n tra tio n (ngA.) VoRecovery 2 0 2 NA 19.3 NA 107 104 19.8 ngA. 4 .6% FBSA Concentration (ngA.) VoRecovery 27.6 NA 2 6 4 NA 109 90.2 27.0 ngA. 4 4 % 1 I oN'P- 3M U M S ID Description IS012-01-01-03-140 DAL-SW-T707-0-121204 IS 012-01-01-03-141 DAL-SW-T707-DB-121204 ISO12-01-01-03-142 DAL-SW-T707-FMS-121204 A verage Concentration (ngA.) % R PD/% R SD NA = Not Applicable FOSA C o n c e n tra tio n (ngA.) VoRecovery <2 00 NA <2.00 NA 9 7 2 107 <2.00 ng/L MeFOSAA Concentration (ngA.) VoRecovery <2.00 NA <2.00 NA 81.9 90.3 <2.00 ng/L EtFOSAA C o n c e n tra tio n (ngA.) <5.00 NA <5.00 NA 81.0 89.3 <5.00 ng/L PAGE 48 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 24. DALSW T808 3M U M S ID Description IS012-01-01-03-153 DAL-SW-T808-0-121205 IS012-01-01-03-154 DAL-SW-T808-DB-121205 IS012-01-01-03-155 DAL-SW-T808-FMS-121205 Average Concentration (ng/L) %RPD 3M U M S ID Description IS012-01-01-03-153 DAL-SW-T808-0-121205 IS012-01-01-03-154 DAL-SW-T808-DB-121205 IS012-01-01-03-155 DAL-SW-T808-FMS-121205 A v e ra g e C oncentration (ng/L) YoRPD PFBA C o n c e n tra tio n (ng/L) YoRecovery 6 2 8 NA 6.89 NA 96.0 89.4 6.59 ng/L 9.3% PFHxA PFHpA Concentration (ng/L) YoRecovery 4.51 3.85 112 NA NA 108 4.18 ng/L 1 6 % C o n c e n tra tio n (ng/L) YoRecovery <2.00 <2.00 106 NA NA 106 <2.00 ng/L PFOA Concentration (ng/L) YoRecovery 7.49 6.79 96.9 NA NA 937 7.14 ng/L 9 .8 % PFBS C o n c e n tra tio n (ngA.) YoRecovery 11.2 NA 10.9 NA 109 98.0 11.1 ng/L 2 7 % PFHS PFOS Concentration (ngA.) YoRecovery <5.00 NA <5.00 NA 94.4 94.4 <5.00 ng/L C o n c e n tra tio n (ngA.) YoRecovery 13.3 NA 14.3 NA 101 94.1 1 3 .8 ng/L 7 .2% FBSA Concentration (ngA.) YoRecovery 25.7 NA 25.7 NA 107 81.3 25.7 ngA. 0 .0% 1 I NP O' 1 i 3M U M S ID Description IS012-01-01-03-153 DAL-SW-T808-0-121205 IS012-01-01-03-154 DAL-SW-T808-DB-121205 IS012-01-01-03-155 DAL-SW-T808-FMS-121205 A v erag e C oncentration (ng/L) YoRPD/YoRSD NA = Not Applicable FOSA C o n c e n tra tio n (ngA.) <2.00 NA <2.00 NA 102 102 <2.00 ng/L MeFOSAA Concentration (ngA.) <2.00 <2.00 89.9 NA NA 90.1 <2.00 ng/L 5? !1 EtFOSAA C o n c e n tra tio n (ngA.) <5.00 <5.00 89.4 NA NA 89.6 <5.00 ng/L PAGE 49 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 25. DALSW T909 3M U M S ID Description IS 012-01-01-03-166 DAL-SW-T909-0-121205 IS012-01-01-03-167 DAL-SW-T909-DB-121205 IS012-01-01-03-168 DAL-SW-T909-FMS-121205 Average Concentration (ng/L) %RPD 3M U M S ID Description IS 012-01-01-03-166 DAL-SW-T909-0-121205 IS012-01-01-03-167 DAL-SW-T909-DB-121205 IS012-01-01-03-168 DAL-SW-T909-FMS-121205 A v e ra g e C oncentration (ng/L) YoRPD PFBA C o n c e n tra tio n (ng/L) YoRecovery 8.66 8.97 99.5 NA NA 90.7 8.82 ng/L 3.5% PFHxA PFHpA Concentration (ng/L) YoRecovery 7.12 6.28 110 NA NA 103 6.70 ng/L 1 3 % C o n c e n tra tio n (ng/L) YoRecovery 3.37 3.75 109 NA NA 105 3.56 ng/L 11Yo PFOA Concentration (ng/L) YoRecovery 11.6 NA 109 NA 108 101 11.3 ng/L 6 .2 % PFBS C o n c e n tra tio n (ngA.) YoRecovery 12 8 NA 14.0 NA 112 98.6 13.4- ng/L 9.0Yo PFHS PFOS Concentration (ngA.) YoRecovery <5.00 NA <5.00 NA 97.1 97.1 <5.00 ng/L C o n c e n tra tio n (ngA.) YoRecovery 192 NA 19.4 NA 117 105 19.3 ngA. 1.0% FBSA Concentration (ngA.) YoRecovery 46.3 NA 45.6 NA 125 79.1 46.0 ng/L 1 .5 % t wO1' 1 i 3M U M S ID Description IS 012-01-01-03-166 DAL-SW-T909-0-121205 IS012-01-01-03-167 DAL-SW-T909-DB-121205 IS012-01-01-03-168 DAL-SW-T909-FMS-121205 A v erag e C oncentration (ngA.) YoRPD/YoRSD NA = Not Applicable FOSA C o n c e n tra tio n (ngA.) <200 NA <2.00 NA 94.4 94.4 <2.00 ng/L MeFOSAA Concentration (ngA.) <2.00 NA <2.00 NA 90.8 91.0 <2.00 ng/L 5? ! 1 EtFOSAA C o n c e n tra tio n (ngA.) <5.00 <5.00 98.8 NA NA 99.0 <5.00 ng/L PAGE 50 OF 52 3M Environmental Laboratory Report No IS012-01-01-03 Table 26. DAL SW TRIP BLANK PFBA 3M U M S ID Description IS 012-01-01-03-061 DAL-SW-TRI P02-0-121116 (1) IS 012-01-01-03-171 DAL-SW-TRI P-01-FMS-121203 Concentration (ng/L) 92 5 90.1 % R ecovery 92 5 90.1 PFHxA PFHpA Concentration (ng/L) 104 112 % Recovery 104 112 C o n c e n tra tio n (ng/L) 95.0 94.3 "/R ecovery 95.0 94.3 PFOA Concentration (ng/L) 95.4 94.2 % R ecovery 99.6 98.3 PFBS 3M U M S ID IS 012-01-01-03-061 IS 012-01-01-03-171 Description DAL-SW-TRI P02-0-121116 (1) DAL-SW-TRI P-01-FMS-121203 C o n c e n tra tio n (ngA.) 99.3 97.9 % Recovery 99.3 97.9 PFHS Concentration (ng) 97.4 933 % R eco very 97.4 933 PFOS C o n c e n tra tio n (ng/L) 89.7 84.1 % R ecovery 96.8 90.7 FBSA Concentration (ngA.) 97.6 102 % R ecovery 97.6 102 FOSA 3M U M S ID Description IS 012-01-01-03-061 DAL-SW-TRI P02-0-121116 (1) IS 012-01-01-03-171 DAL-SW-TRI P-01-FMS-121203 C o n c e n tra tio n (ng) 97.6 102 "/ R e c o v e r y 97.6 102 MeFOSAA Concentration (ng) 92 3 99.5 % R ecovery 92.5 99.7 EtFOSAA C o n c e n tra tio n (ng/L) 97.2 99.1 % R ecovery 97.4 99.3 (1) Sample IS012-01-01-03-061 identified as DAL-SW-TRIP02-0-121116 is a trip blank FMS sample. The naming of the sample was not consistent with the set of Trip Blank samples collected on December 3,2012. PAGE 51 OF 52 3M Environmental Laboratory Report No. IS012-01-01-03 5 Conclusion Laboratory control spikes w ere used to determ ine the analytical m ethod accuracy and precision for P F O S . T h e accuracy and precision w ere then used to estim ate the m ethod uncertainty for the results. Field matrix spike recoveries dem onstrated that the analytical m ethod w as appropriate for the given sam ple matrix. Analysis w as com pleted using 3M Environm ental Laboratory m ethod E T S -8 -0 4 4 .1 "M etho d of A nalysis for the D eterm ination of Perfluorinated C o m p o u n d s in W a te r by L C /M S /M S ; D irect Injection A n alysis". Analytical results are reported in T a b le s 1 and 1 3 -2 6 of this report. 6 D ata/S am ple R eten tion All rem aining sam p le s and associated project d ata (hardcopy and electronic) will b e archived according to 3M Environm ental Laboratory standard operating procedures. W illiam K. R e a g e n , P h .D ., 3 M E nvironm ental Laboratory M a n a g e r D ate T h e 3 M E nvironm ental Laboratory's Q uality A ssu ran ce U nit has audited the d a ta and report for this project. j? - ' 2 nee Representative D a te PAGE 52 OF 52 3 Analytical Report Determination of PFC Concentrations in Fish Collected from the Tennessee River in the Fall of 2012 Study Title Analytical Study ISO12-01-01-04: Tennessee River Fish Sample Analysis, October 2012 Project Coordinator(s) Jai Kesari, P.E., DEE and Charles Young Weston Solutions, Inc. 1400 Weston Way Westchester, MA 19380 Principal Analytical Investigator Cleston C. Lange, Ph.D. 3M Environmental Laboratory Report Completion Date Date of Final Signature Analytical Laboratory 3M Environmental Health and Safety Operations Environmental Laboratory 3M Center, Bldg 260-05-N-17 St. Paul, MN 55144 Project Identification ISO12-01-01-04 Total Number of Pages,. 39 This laboratory maintains A2LA accreditation to: ANSI/ISO/IEC 17025:2005for the specific tests/calibrations listed in A2LA Certificate # 2052.01 and meets the principles o fISO 9001:2000. The test results included in this report are covered by this accreditationfollowing method ETS-8-045.1 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Table of C ontents T a b le o f C o n te n ts ..........................................................................................................................................................................2 List o f T a b le s .................................................................................................................................................................................... 3 1 Study In fo rm a tio n ..................................................................................................................................................................4 2 S u m m a r y ..................................................................................................................................................................................5 3 In trod u ction ............................................................................................................................................................................15 4 R e fe re n c e S u b s ta n c e s ......................................................................................................................................................16 5 M etho d S u m m a ry ............................................................................................................................................................... 19 5.1 M e th o d s .................................................................................................................................................................. 19 5 .2 S a m p le R e c e ip t................................................................................................................................................... 19 5 .3 S a m p le P rep ara tio n /E x tra c tio n ..................................................................................................................... 2 0 5 .4 L C /M S /M S A n a ly s is ........................................................................................................................................... 2 0 6 Results ................................................................................................................................................................................... 2 4 6.1 Calibration ............................................................................................................................................................ 2 4 6 .2 Limits o f Q uantitation ( L O Q s ) ........................................................................................................................2 5 6 .3 Continuing C a lib ra tio n .......................................................................................................................................2 5 6 .4 B lan ks.......................................................................................................................................................................2 6 6 .5 Laboratory Control S a m p le s (L C S s )........................................................................................................... 2 7 6 .6 Laboratory M atrix S pikes ( L M S s ) ................................................................................................................ 34 6 .7 N IS T S R M 1 9 4 7 R e s u lts ..................................................................................................................................34 6 .8 Fillet E qu ipm ent A q u eo u s R in seate R e s u lts ............................................................................................34 7 C o n c lu s io n .............................................................................................................................................................................36 8 D a ta /S a m p le R e te n tio n .................................................................................................................................................... 37 9 List o f A tta c h m e n ts .............................................................................................................................................................37 10 S ig n a tu re s .............................................................................................................................................................................. 37 2 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 List of Tables T a b le 1. C oncentration Results for T e n n e s s e e R iver Fish T issu e S a m p le s ........................................................6 T a b le 2. Laboratory M atrix S pike (L M S ) R e c o v e rie s ................................................................................................... 14 T a b le 3. R e fe re n c e S ubstances: T a rg e t A n a ly te s ........................................................................................................ 16 T a b le 4. R e fe re n c e S ubstances: S tab le Isotope Labeled Internal S tan dards ( I S ) ......................................... 17 T a b le 5. R e fe re n c e S ubstances: S tab le Isotope Labeled S urrogate R ecovery S tan dards ( S R S ) .........19 T a b le 6. L C /M S /M S Instrum ent Inform ation (E T S -8 -0 4 5 ).......................................................................................... 21 T a b le 7. G rad ien t Liquid C h rom ato graph y Conditions (E T S -8 -0 4 5 ); C 7- C 12 P F C A s , P F S A s and S R S s ...................................................................................................................................................................................... 21 T a b le 8. G rad ien t Liquid C h rom ato graph y Conditions (E T S -8 -0 4 5 ); for C 4-C 6 P F C A s ................................ 2 2 T a b le 9. A nalytes, Surrogates, Internal S tan dards, and Transitions..................................................................... 2 2 T a b le 10. Analytical B a tc h e s ..................................................................................................................................................2 3 T a b le 11. L C S R e s u lts .............................................................................................................................................................2 8 T a b le 12. C oncentration Results for Fillet-Process E qu ipm ent R in seate B la n k s ............................................35 T a b le A 1 . C oncentration R esults for Bass & Catfish Fillet Control M aterials (R ight Side F illets).............. 38 3 1 Study Information Sponsor 3M Company Project Requestor Gary Hohenstein Manager, Environm ental/ Regulatory Affairs Special Projects, EHS Operations Bldg 224-5W -03 St. Paul, MN 55144 Phone: (651) 737-3570 gahohenstein@ m m m .com Project Coordinator(s) Jai Kesari, P.E., DEE Charles Young W eston Solutions, Inc. 1400 W eston W ay W estchester, MA 19380 Jai phone: 610-701-3761 Charles phone: 610-701-3787 Jai.kesari@ westonsolutions.com C harles.young@ westonsolutions.com Principal Analytical Investigator (PAI) C leston C. Lange, Ph.D. 3M Environm ental Laboratory 3M Center, Bldg 260-5N-17 St. Paul, MN 55144 Phone: (651)-733-9860 clange@ m m m .com Analytical Testing Facility 3M EHS Operations 3M Environm ental Laboratory Building 260-5N-17 St. Paul, MN 55144 Study Personnel (3M Environmental Laboratory) M arlene Heying, A nalyst (Pace Analytical, Professional Services) Jon Steege, Analyst (Pace Analytical, Professional Services) Patrick Kenney, Analyst (Pace Analytical, Professional Services) Kevin Eich, A na lyst (Q uality A ssociates, Inc., Professional Services) Laura Harrington, A nalyst (Pace Analytical, Professional Services) Study Dates S tudy Initiation: O ctober 12, 2012 A n a lytica l Initiation: D e ce m b e r 5, 2012 A nalytical C om pletion: February 6, 2012 Report Com pletion: Date of Final Signature ISO12-01-01-04 Tennessee River Fish Study Fall 2012 4 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 2 Summary Reported herein are the determ ined concentrations of certain perfluorinated chem icals (P FC s) m easu red in fish collected from th e T e n n e s s e e R iver in th e Fall o f 2 0 1 2 . T h e s e analyses w e re conducted as 3 M E nvironm ental analytical project IS O 1 2 -0 1 -0 1 -0 4 and w a s perform ed in support o f the W e s to n Solutions, Inc. study plan, titled "R evised W o rk Plan for Fall 2 0 1 2 Fish and S urface W a te r Sam pling in th e T e n n e s s e e R iver", and dated O cto b er 2 0 1 2 . During the Fall of 2 0 1 2 , W eston Solutions, Inc. personnel caught five largem outh bass and five catfish from each of fifteen pre-defined fish sam pling reaches located within the confines of the W ilson and W h e e le r R eservoirs o f th e T e n n e s s e e River, located n e a r D ecatur, A L. T h e fish w e re filleted (skin rem oved ) in th e field and provided blind to the laboratory in term s o f specific information on fish collections. A total of 175 fish tissue sam ples w ere received and quantitatively analyzed. T h e P FC s analyzed w ere perfluorobutanoate (PFBA ), perfluorohexanoate (PFH xA ), perfluoroheptanoate (PFH pA ), perfluorooctanoate (PFO A ), perfluorobutane sulfonate (P FB S ), perfluorohexanesulfonate (P F H S ), perfluorooctanesulfonate (P F O S ) , perfluorobutanesulfonamide (FB SA ), perfluorooctanesulfonamide (F O S A ),2-[N m ethyl-FO SA ]acetic acid (M e F O S A A ) and 2-[N -ethyl-FO S A ]acetic acid (E tF O S A A ) and results reported in Table 1. E ach s am p le w a s extracted in duplicate for determ ination o f th e a v e ra g e P F C concentrations, and approxim ately every10th fish s a m p le (21 in total; 12 % o f total fish) had tw o laboratory matrix spike (L M S ) sam p les prepared from a third and fourth 0.5 gram aliquot and fortified with known quantities of target analytes. T h e LM S recovery results w ere used as a m easure of the sa m p le m e a s u re m e n t accuracy. T h e av e ra g e recoveries o f L M S s for all analytes w e re within 100 + 1 0 % , except F B S A which w as 8 2 .3 % . T h e averag e recovery for P F O S w as 9 7 .2 % with an R S D o f 8 .6 % . T h e L M S recovery results are show n in Table 2. Each s am p le preparation also received two surrogate recovery standards (S R S s ) 13C 4-P F O A and 13C 4-P F O S . T h e recovery o f 13C 4-P F O S w a s 8 8 .0 % (R S D = 3 .8 % ) and for 13C 4-P F O A w a s 8 5 .2 % (R S D = 5 .2 % ). T h e overall R S D s determ ined from the S R S recovery results for sam ples w ere used as a m easure of the analytical m ethod precision and indicated excellent precision. Laboratory control spikes (L C S s ) w e re prepared at 3 levels, each level in triplicate, and analyzed with each analytical batch. A v e ra g e L C S recoveries w e re within 1 0 0 + 1 4 % at all levels, and overall, for each analyte. T h e L C S statistical results (for all L C S levels) w e re used to determ ine the analytical m ethod uncertainty (95% C I) at + 18% for PFB A , + 15% for P FH xA , + 21 % for PFH pA, + 20% for P FO A , + 18% for PFB S, + 19% for P FH S , + 22% for total-PFO S, + 40% for FBSA, + 20% for FO S A , + 18% for M eF O S A A and + 19% for EtFO SAA . Overall, the sam ple results show ed that P F O S , F B S A and F O S A w ere detected with highest frequencies in th e T e n n e s s e e R iver fish fillet tissue s am p les, with P F O S detected in ~ 9 8 % of the sam p les received, and F B S A and F O S A detected in ~ 8 0 -9 0 % o f th e s am p les. P F O S w as m easu red at th e highest concentration o f all analytes with a m axim u m valu e o f 5 9 9 ng/g (w et weight; w w ) followed by FB S A with a m axim um m easured value of 5 67 ng/g (ww) and F O S A m axim um m easured value of 123 ng/g (ww). M e F O S A A and E tF O S A A w ere detected at m oderate frequency of ~ 5 0 % , and lower m axim um levels m easured at 2 2 .0 and 19.5 ng/g, respectively. P F B S w a s detected at ~ 2 0 % frequency in sam p les, with a m axim u m m easu red concentration of 3 .8 2 ng/g (ww). T h e rem aining analytes PFB A , P FH xA , P FH pA , P FO A , and P F H S w ere detected infrequently at less than 15% of sam ples and m axim um m easured values w ere lower than P FB S m axim um value of 3 .8 2 ng/g (ww). 5 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Table 1. C o n c e n tra tio n R e s u lts fo r T e n n e s s e e R iv e r Fish T is s u e S a m p le s SAMPLE ID LIMS ID TN _11-01941_1 S a m p le 1 P re-S tudy H o m o g e n iz a tio n Process c o n tro l MSFOO1 IS O 12-01-01-04-001 IS O 12-01-01-04-002 IP F 0 0 2 IS O 12-01-01-04-003 IP F 0 0 3 IS O 1 2-0 1-0 1-0 4-0 04 M SF004 IS O 12-01-01-04-005 M SF005 IS O 1 2-0 1-0 1-0 4-0 06 M SF006 IS O 1 2-0 1-0 1-0 4-0 07 IP F007 IS O 1 2-0 1-0 1-0 4-0 08 IP F 0 0 9 IS O 12-01-01-04-009 M SF010 IS O 1 2-0 1-0 1-0 4-0 10 M SF011 IS O 12-01-01-04-011 IP F 0 1 2 IS O 12-01-01-04-012 M SF013 IS O 12-01-01-04-013 IP F014 IS O 1 2-0 1-0 1-0 4-0 14 M SF015 IS O 12-01-01-04-015 IP F016 IS O 1 2-0 1-0 1-0 4-0 16 M SF017 IS O 1 2-0 1-0 1-0 4-0 17 IP F018 IS O 1 2-0 1-0 1-0 4-0 18 PFBA 1.42 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 Average Concentration (ng/g; w et w t.) PFHxA PFHpA PFOA PFBS PFHS PFOS* FBSA Avg. SRS Recovery FOSA MeFOSAA EtFOSAA 13CC4PFOA 13CC4PFOS < 0 .1 0 1 <0.503 < 0 .9 6 0 <0.101 < 0 .0 9 6 0 0 .7 3 1 < 0 .2 5 0 [c] <0.253 < 0 .5 0 0 < 0 .5 0 0 107% 105% < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 0 .5 7 6 [a,c] < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 1 .1 0 < 1 .1 0 3.82 < 1 .1 0 < 1 .1 0 < 1 .1 0 < 1 .1 0 < 1 .1 0 < 1 .1 0 NR < 1 .1 0 < 1 .1 0 < 1 .1 0 < 1 .1 0 < 1 .1 0 < 1 .1 0 < 1 .1 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 259 8.49 18.3 167 168 173 8 .8 6 13.4 71.0 98.5 12.1 68.9 14.3 67.1 14.6 79.6 11.7 8 6 .2 [b , c] 20.2 [c] 1 6 4 [b, c] 5 8 .2 [c ] 6 0 .9 [c ] 7 9 .5 [c ] 4 .8 3 [c ] 5 .2 9 [c ] 6 8 .6 [c ] 8 1 .7 [c] 9 6 .3 [b , c] 8 4 .1 [c ] 5 2 .4 [c] 56.2 [c] 55.5 [c] 51.3 [c] 2 8 .6 [c] 2.37 0.9 4 7 [b] 2.23 3.05 3.08 2.26 <0.505 0 .4 8 7 1.42 2.03 4.81 1.37 2.35 1.24 2.40 1.09 1.21 1.45 < 0 .5 0 0 1.07 1.37 1.35 0 .9 6 2 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 0 .8 2 9 0 .5 4 6 [a] 0 .6 5 4 [a] 0 .6 8 4 [a] 0 .6 9 6 [a] < 0 .5 0 0 < 0 .5 0 0 1.19 < 0 .5 0 0 0 .7 1 4 1.17 1.13 0 .9 4 3 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 0 .5 1 1 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 89.7% 86.1% 84.4% 86.3% 85.1% 84.4% 88.3% 86.8% 83.0% 89.0% 86.5% 85.6% 84.4% 81.8% 86.8% 87.1% 85.0% 92.6% 88.0% 87.1% 87.9% 87.1% 87.7% 89.3% 88.8% 87.4% 91.7% 90.7% 87.7% 87.5% 85.7% 89.1% 92.0% 87.9% 6 Table 1. C o n c e n tra tio n R e s u lts fo r T e n n e s s e e R iv e r Fish T is s u e S a m p le s ISO12-01-01-04 Tennessee River Fish Study Fall 2012 SAMPLE ID M SF020 M SF021 IP F 0 2 2 M SF023 IP F024 M SF025 IP F026 M SF027 M SF028 IP F 0 2 9 M SF030 IP F 0 3 1 M SF033 IP F034 M SF035 IP F036 M SF037 M SF038 IP F 0 3 9 M SF040 IP F 0 4 1 M SF042 M SF043 M SF044 M SF045 LIMS ID IS O 1 2-0 1-0 1-0 4-0 20 IS O 12-01-01-04-021 IS O 12-01-01-04-022 IS O 12-01-01-04-023 IS O 1 2-0 1-0 1-0 4-0 24 IS O 12-01-01-04-025 IS O 1 2-0 1-0 1-0 4-0 26 IS O 1 2-0 1-0 1-0 4-0 27 IS O 1 2-0 1-0 1-0 4-0 28 IS O 12-01-01-04-029 IS O 1 2-0 1-0 1-0 4-0 30 IS O 12-01-01-04-031 IS O 12-01-01-04-032 IS O 12-01-01-04-033 IS O 1 2-0 1-0 1-0 4-0 34 IS O 12-01-01-04-035 IS O 1 2-0 1-0 1-0 4-0 37 IS O 1 2-0 1-0 1-0 4-0 38 IS O 12-01-01-04-039 IS O 1 2-0 1-0 1-0 4-0 40 IS O 12-01-01-04-041 IS O 12-01-01-04-042 IS O 12-01-01-04-043 IS O 1 2-0 1-0 1-0 4-0 44 IS O 12-01-01-04-045 PFBA < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 2 .3 4 [a] < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 NR < 0 .1 0 0 < 0 .1 0 0 PFHxA < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 1.78 [a] < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 PFHpA <0.252 <0.252 <0.252 <0.101 <0.101 <0.101 1.79 [a] <0.101 <0.101 <0.101 0 .1 9 8 [a] <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0 .1 2 0 [a] <0.101 0 .1 1 8 [a] <0.101 Average Concentration (ng/g; w et w t.) Avg. SRS Recovery PFOA < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 3.13 [a] < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 1.57 [a] NR < 0 .2 4 0 < 0 .2 4 0 PFBS < 1 .1 0 < 1 .1 0 < 1 .1 0 0 .1 8 4 [b] 2 .6 6 8 0 .2 9 4 [a] 0 .8 8 7 [b] 0 .1 7 6 [b] 0 .8 5 9 0 .4 6 0 0 .5 9 0 1.31 0 .4 5 9 1.862 0 .2 1 9 0.371 [b] 0.582 [b] 0 .9 9 6 [b] 0.305 [b] 0 .2 5 9 0.405 [b] 0.131 [b] 0 .1 3 8 [b] <0.252 <0.252 PFHS < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 1.54 [a] < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 PFOS* 65.8 98.2 5.10 54.9 10.3 66.4 45.5 [b] 65.3 65.9 18.6 65.2 21.9 190 8.12 207 14.4 1 0 1 .9 106 15.7 104 22.2 210 [b] 224 116 225 FBSA FOSA MeFOSAA EtFOSAA 5 6 .7 [c] 23.3 [c] 36.9 [c] 3 3 .0 [c] 12.3 [c] 4 7 .9 [b , c] 19.6 [c] 4 8 .1 [c] 4 5 .0 [c] 69.2 [c] 22.1 [c] 8 4 .2 [c] 31.5 [c] 5 .3 8 [c] 4 1 .3 [c] 7 .8 6 [c] 6 3 .6 [c] 6 6 .4 [c] 5 3 .7 [c] 3 5 .6 [c] 3 5 .4 [c] 5 2 .8 [c] 55.5 [c] 107 37.9 1.11 0 .6 9 9 <0.505 0 .8 0 0 1.07 [b] 0 .8 8 7 6.38 0 .9 8 8 0 .9 7 0 4 .2 0 0 .5 7 1 3.01 1.12 0.8 8 4 [b] 1.28 0 .6 2 9 0 .7 1 6 0 .7 5 0 3.58 1.26 2.41 1.25 1.32 3.09 1.99 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 2.4 0 [a] < 0 .5 0 0 < 0 .5 0 0 1.94 < 0 .5 0 0 0 .7 2 9 1.67 < 0 .5 0 0 0 .5 9 6 < 0 .5 0 0 < 0 .5 0 0 0 .5 2 3 0 .7 1 6 0 .7 9 8 1.30 2.61 [b] 2.86 1.06 0.768 [b] < 0 .5 0 0 0 .5 2 0 [a] < 0 .5 0 0 0 .3 2 6 0 .2 6 0 [a] 0 .2 8 2 4 .7 3 [a] 0 .3 4 4 [b] 0 .3 3 7 0 .8 4 4 0 .3 8 8 [b] 0 .2 8 1 0 .8 4 5 < 0 .2 5 0 0 .6 0 2 < 0 .2 5 0 0 .4 2 5 0 .4 6 4 0 .3 7 3 0 .6 5 2 0 .3 8 1 1.52 1.62 0 .7 9 1 0 .7 7 7 13CC4PFOA 84.2% 85.7% 87.6% 84.7% 85.4% 85.7% 83.4% 92.7% 87.5% 87.6% 97.3% 87.8% 85.2% 85.0% 89.0% 87.9% 84.8% 88.6% 88.0% 84.7% 85.5% 82.5% 94.1% 85.2% 83.4% 13CC4PFOS 87.5% 86.2% 88.6% 85.8% 86.1% 89.2% 84.3% 92.9% 90.2% 85.8% 100% 87.0% 86.4% 85.2% 90.2% 87.9% 84.6% 88.8% 89.6% 87.6% 86.0% 82.5% 96.0% 90.7% 89.2% 7 Table 1. C o n c e n tra tio n R e s u lts fo r T e n n e s s e e R iv e r Fish T is s u e S a m p le s ISO12-01-01-04 Tennessee River Fish Study Fall 2012 SAMPLE ID LIMS ID M SF046 IP F047 M SF048 IP F 0 4 9 M SF050 IP F 0 5 1 M SF052 IP F 0 5 3 IP F 0 5 5 M SF056 IP F057 M SF058 IP F 0 5 9 M SF060 M SF061 M SF062 IP F 0 6 3 M SF065 IP F066 M SF067 IP F068 IP F 0 6 9 IP F070 M SF071 M SF073 IS O 1 2-0 1-0 1-0 4-0 46 IS O 1 2-0 1-0 1-0 4-0 47 IS O 1 2-0 1-0 1-0 4-0 48 IS O 12-01-01-04-049 IS O 1 2-0 1-0 1-0 4-0 50 IS O 12-01-01-04-051 IS O 12-01-01-04-052 IS O 12-01-01-04-053 IS O 12-01-01-04-055 IS O 1 2-0 1-0 1-0 4-0 56 IS O 1 2-0 1-0 1-0 4-0 57 IS O 1 2-0 1-0 1-0 4-0 58 IS O 12-01-01-04-059 IS O 1 2-0 1-0 1-0 4-0 60 IS O 12-01-01-04-061 IS O 12-01-01-04-062 IS O 12-01-01-04-063 IS O 1 2-0 1-0 1-0 4-0 64 IS O 12-01-01-04-065 IS O 1 2-0 1-0 1-0 4-0 66 IS O 1 2-0 1-0 1-0 4-0 67 IS O 1 2-0 1-0 1-0 4-0 68 IS O 12-01-01-04-069 IS O 1 2-0 1-0 1-0 4-0 70 IS O 12-01-01-04-072 PFBA < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 PFHxA < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 PFHpA <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0 .1 7 6 [a] <0.101 0 .1 3 8 [a] <0.101 <0.101 <0.101 Average Concentration (ng/g; w et w t.) PFOA < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 PFBS <0.252 0 .3 2 7 0 .2 6 9 [a] <0.252 <0.252 0 .3 3 7 <0.252 1 .5 4 [c] <0.252 <0.252 <0.252 <0.252 0 .3 2 7 <0.252 0 .2 8 9 <0.252 0 .8 5 4 <0.252 0.353 [b] <0.252 <0.252 0 .7 3 9 <0.252 <0.252 <0.252 PFHS < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 PFOS* 54.1 14.3 88.9 5.59 8 1 .6 8.01 93.5 [b] 6.68 11.0 54.8 7.33 75.3 7.56 64.7 75.5 103 6.67 86.9 10.5 [c] 9 4 .6 9 .6 0 5.63 1.81 35.8 36.3 FBSA 56.5 63.9 129 20.6 116 28.0 71.1 15.9 31.5 19.3 22.8 69.7 26.1 74.2 85.9 9 2 .6 16.0 4 6 .8 37.5 38.6 4 0 .8 18.0 < 0 .2 5 0 0 .5 5 5 0 .5 2 9 Avg. SRS Recovery FOSA MeFOSAA EtFOSAA 1.11 2.71 2.40 1.29 2.11 1.71 2.11 1.31 1.37 [b] 0 .5 3 0 1.58 1.59 1.69 2.48 2.21 2.42 1.43 1.19 1.95 1.98 1.48 0 .7 7 1 0.153 [b] 0 .1 3 7 0 .1 6 1 0 .4 3 5 0 .2 9 3 1.00 < 0 .2 5 0 0 .8 8 3 0 .3 0 7 0.448 [b] < 0 .2 5 0 0.333 [b] < 0 .2 5 0 < 0 .2 5 0 0 .4 0 4 < 0 .2 5 0 0.365 [b] 0 .3 9 3 0 .4 1 9 0 .3 0 0 0 .3 3 1 0 .1 7 4 0 .3 1 3 0 .1 9 8 0 .1 4 2 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 0 .3 1 5 0 .1 1 6 [a] 0 .7 9 5 0.1 4 1 [a] 0 .7 3 1 < 0 .1 0 0 0 .4 5 8 < 0 .1 0 0 0.1 1 3 [b] 0 .2 2 6 [b] 0.1 0 2 [a] 0 .3 8 4 < 0 .1 0 0 0 .4 4 7 0 .5 4 4 0 .4 5 7 0 .1 4 7 0 .2 8 4 0 .3 4 4 0 .4 9 3 0 .2 7 9 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 13CC4PFOA 83.3% 85.3% 83.5% 85.5% 87.0% 81.4% 84.2% 85.0% 84.9% 82.1% 80.9% 81.6% 81.5% 77.4% 83.1% 81.4% 81.5% 80.8% 92.5% 81.2% 91.2% 91.3% 84.7% 81.2% 84.2% 13CC4PFOS 89.3% 90.8% 90.7% 92.6% 89.7% 87.2% 87.9% 89.6% 87.3% 87.9% 84.6% 86.4% 85.4% 84.4% 87.7% 88.1% 82.3% 83.2% 96.2% 85.7% 91.7% 93.0% 85.4% 89.8% 86.6% 8 Table 1. C o n c e n tra tio n R e s u lts fo r T e n n e s s e e R iv e r Fish T is s u e S a m p le s ISO12-01-01-04 Tennessee River Fish Study Fall 2012 SAMPLE ID M SF074 M SF075 IP F076 IP F077 IP F078 IP F 0 7 9 M SF080 M SF081 IP F 0 8 2 IP F 0 8 3 M SF084 M SF085 M SF086 M SF087 M SF089 M SF090 M SF091 M SF092 M SF094 IP F 9 5 M SF096 IP F 9 7 IP F 9 8 M SF099 IP F100 LIMS ID PFBA IS O 12-01-01-04-073 IS O 1 2-0 1-0 1-0 4-0 74 IS O 12-01-01-04-075 IS O 1 2-0 1-0 1-0 4-0 76 IS O 1 2-0 1-0 1-0 4-0 77 IS O 1 2-0 1-0 1-0 4-0 78 IS O 12-01-01-04-079 IS O 1 2-0 1-0 1-0 4-0 80 IS O 12-01-01-04-081 IS O 12-01-01-04-082 IS O 12-01-01-04-083 IS O 1 2-0 1-0 1-0 4-0 84 IS O 12-01-01-04-085 IS O 1 2-0 1-0 1-0 4-0 86 IS O 1 2-0 1-0 1-0 4-0 87 IS O 1 2-0 1-0 1-0 4-0 88 IS O 1 2-0 1-0 1-0 4-0 90 IS O 12-01-01-04-091 IS O 12-01-01-04-092 IS O 12-01-01-04-093 IS O 1 2-0 1-0 1-0 4-0 94 IS O 12-01-01-04-095 IS O 1 2-0 1-0 1-0 4-0 96 IS O 1 2-0 1-0 1-0 4-0 97 IS O 1 2-0 1-0 1-0 4-0 98 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 0 .8 6 0 [a] < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 PFHxA PFHpA < 0 .0 5 0 3 < 0 .0 5 0 3 0 .1 3 9 [a] < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 0 .1 3 7 [a] < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .0 5 0 3 < 0 .1 0 1 0 .2 6 0 [a] 0 .1 5 1 [a] 0 .1 7 4 [a] < 0 .1 0 1 0 .1 3 2 0 .0 9 6 5 [a] 0 .1 4 4 0 .1 3 7 [a] < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0 .1 1 0 [a] <0.101 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 Average Concentration (ng/g; w et w t.) Avg. SRS Recovery PFOA < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 PFBS <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 PFHS < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 PFOS* 32.7 27.9 0 .7 6 8 1.50 5.17 0 .8 3 6 18.6 37.8 2.92 3.41 [b] 44.1 32.6 49.5 4 9 .0 4 0 .8 104 159 434 69.0 4 .3 7 46.9 4 .3 7 2.22 124 4.69 FBSA 1.72 0 .6 4 8 < 0 .2 5 0 < 0 .2 5 0 3.58 < 0 .2 5 0 0 .3 6 1 0 .8 0 2 0 .2 9 5 0.512 [b] 7.01 2.72 6.87 25.8 14.8 28.4 20.4 8.55 28.1 1.16 2.95 1.90 0 .8 6 7 6.10 0 .5 7 5 FOSA MeFOSAA EtFOSAA 0 .2 1 5 0 .1 2 2 <0.101 <0.101 0.2 7 7 [b] <0.101 0 .1 4 8 0 .1 3 5 [a] 0 .1 9 6 [a] 0 .1 2 3 [a] 1.44 0 .5 8 0 0 .1 2 7 [a] 0 .3 7 9 1.22 [b] 1.71 2.24 4.69 0 .6 7 2 0 .2 5 8 0 .3 9 6 0 .4 1 3 0 .2 9 9 [a] 1.60 0 .6 5 6 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 0 .1 4 9 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 0 .3 9 8 0.122 [b] < 0 .1 0 0 0 .2 8 4 0.288 [b] 0 .2 8 9 0 .4 7 8 0.688 [b] 0 .4 4 6 < 0 .1 0 0 0 .1 7 1 0 .1 2 1 < 0 .1 0 0 0 .6 4 8 0.1 2 2 [a] < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 0 .5 3 0 [b] < 0 .2 5 0 < 0 .2 5 0 0 .3 1 8 0.3 2 2 [a] 0 .3 5 0 [a] 0 .4 6 4 0 .7 0 4 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 0 .4 7 8 [b] < 0 .2 5 0 13CC4PFOA 88.2% 83.8% 87.7% 85.0% 85.0% 80.9% 81.2% 88.2% 91.1% 82.9% 83.8% 84.6% 76.0% 78.4% 81.1% 85.3% 83.8% 82.9% 82.7% 82.6% 82.9% 80.8% 84.4% 81.3% 81.6% 13CC4PFOS 92.5% 88.7% 87.2% 89.6% 89.0% 82.3% 86.4% 93.8% 92.5% 88.3% 90.6% 88.7% 81.9% 84.0% 88.2% 89.8% 90.1% 87.4% 85.8% 86.5% 87.0% 87.7% 89.2% 86.6% 86.8% 9 Table 1. C o n c e n tra tio n R e s u lts fo r T e n n e s s e e R iv e r Fish T is s u e S a m p le s ISO12-01-01-04 Tennessee River Fish Study Fall 2012 SAMPLE ID LIMS ID IP F 1 0 1 M SF102 IP F 1 0 3 IP F104 IP F 1 0 5 IP F106 IP F107 IP F108 TN _11-01941 _ 1 S a m p le 2 M id -S tu d y H o m o g e n iz a tio n Process c o n tro l M SF109 M SF111 IP F 1 1 2 IP F 1 1 3 IP F114 M SF115 IP F116 IP F117 IP F118 M SF119 IP F120 M SF121 IP F 1 2 2 IS O 12-01-01-04-099 IS O 1 2-0 1-0 1-0 4-1 00 IS O 12-01-01-04-101 IS O 12-01-01-04-102 IS O 12-01-01-04-103 IS O 1 2-0 1-0 1-0 4-1 04 IS O 12-01-01-04-105 IS O 1 2-0 1-0 1-0 4-1 06 IS O 1 2-0 1-0 1-0 4-1 08 IS O 12-01-01-04-109 IS O 1 2-0 1-0 1-0 4-1 10 IS O 12-01-01-04-111 IS O 12-01-01-04-112 IS O 12-01-01-04-113 IS O 1 2-0 1-0 1-0 4-1 14 IS O 12-01-01-04-115 IS O 1 2-0 1-0 1-0 4-1 16 IS O 1 2-0 1-0 1-0 4-1 17 IS O 1 2-0 1-0 1-0 4-1 18 IS O 12-01-01-04-119 IS O 1 2-0 1-0 1-0 4-1 20 IS O 12-01-01-04-121 PFBA < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 1.42 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 PFHxA PFHpA < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 Average Concentration (ng/g; w et w t.) PFOA < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 PFBS <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 PFHS < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 PFOS* 50.9 218 4 2 .0 4.15 3.41 2.42 7.85 29.1 FBSA 3.37 22.4 3.56 1.16 0 .6 3 3 0 .6 0 9 1.39 1.29 Avg. SRS Recovery FOSA MeFOSAA EtFOSAA 6.68 1.37 [b] 6.26 <0.253 0 .2 5 5 [a] 0 .2 7 1 [a] 1.28 1.07 0.236 [b] 1.39 0 .2 4 3 0.1 1 1 [a] < 0 .1 0 0 0 .1 0 7 [a] < 0 .1 0 0 0.246 [b] < 0 .2 5 0 1.27 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 13CC4PFOA 79.9% 82.2% 84.9% 78.2% 85.9% 82.1% 78.7% 82.6% 13CC4PFOS 89.2% 91.2% 91.3% 85.0% 87.7% 88.2% 85.8% 88.0% 0 .1 2 6 [a] <0.503 < 0 .2 4 0 <0.252 < 0 .2 5 0 0 .5 2 3 [a] < 0 .2 5 0 <0.253 < 0 .1 0 0 < 0 .2 5 0 82.9% 92.4% <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 21.8 25.7 1.78 1.30 1.24 31.2 2.65 0 .8 9 7 2.01 23.9 0 .9 6 1 19.9 4.45 1.03 1.57 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 4 .2 8 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 2.26 < 0 .5 0 0 2.06 < 0 .5 0 0 <0.101 0.235 [b] <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 <0.101 0 .1 6 2 0 .3 0 0 0 .1 4 1 <0.101 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 83.8% 84.9% 85.8% 88.9% 84.1% 77.0% 79.6% 86.7% 80.6% 82.1% 82.0% 81.6% 80.6% 86.6% 88.2% 88.6% 92.0% 88.0% 84.1% 85.2% 89.4% 85.2% 91.8% 84.1% 89.6% 83.8% 10 Table 1. C o n c e n tra tio n R e s u lts fo r T e n n e s s e e R iv e r Fish T is s u e S a m p le s ISO12-01-01-04 Tennessee River Fish Study Fall 2012 SAMPLE ID LIMS ID PFBA PFHxA PFHpA IP F 1 2 3 M SF124 M SF125 M SF127 M SF128 M SF129 M SF130 M SF131 M SF132 IP F 1 3 3 IP F134 IP F 1 3 5 IP F137 IP F138 M SF139 M SF140 M SF141 M SF142 M SF143 M SF144 TN _11-01941_1 S a m p le 3 P o st-S tu d y H o m o g e n iz a tio n IP F 1 8 1 M SF145 IS O 12-01-01-04-122 IS O 12-01-01-04-123 IS O 1 2-0 1-0 1-0 4-1 24 IS O 1 2-0 1-0 1-0 4-1 26 IS O 1 2-0 1-0 1-0 4-1 27 IS O 1 2-0 1-0 1-0 4-1 28 IS O 12-01-01-04-129 IS O 1 2-0 1-0 1-0 4-1 30 IS O 12-01-01-04-131 IS O 12-01-01-04-132 IS O 12-01-01-04-133 IS O 1 2-0 1-0 1-0 4-1 34 IS O 12-01-01-04-135 IS O 1 2-0 1-0 1-0 4-1 36 IS O 1 2-0 1-0 1-0 4-1 37 IS O 1 2-0 1-0 1-0 4-1 38 IS O 12-01-01-04-139 IS O 1 2-0 1-0 1-0 4-1 40 IS O 12-01-01-04-141 IS O 12-01-01-04-142 IS O 1 2-0 1-0 1-0 4-1 44 IS O 12-01-01-04-145 IS O 1 2-0 1-0 1-0 4-1 46 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 1 .0 0 < 0 .2 5 0 [c] < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 1.42 < 0 .2 5 0 < 0 .2 5 0 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 <2.52 < 0 .1 0 1 0 .0 9 9 2 [a] 0 .4 1 7 [a] < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 0 .0 9 4 2 [a] < 0 .1 0 1 0 .1 3 6 < 0 .1 0 1 < 0 .1 0 1 0 .1 2 2 [a] 0 .1 7 0 [a] < 0 .1 0 1 < 0 .1 0 1 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 Average Concentration (ng/g; w et w t.) PFOA < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 PFBS <0.252 <0.252 <0.252 0 .4 4 4 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 PFHS < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 0 .4 1 6 < 0 .1 0 0 0 .1 2 3 < 0 .1 0 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 PFOS* 2.11 73.2 40 .8 [d] 408 262 250 333 178 324 22.6 3.98 [b] 4.89 [b] 1.90 8.55 279 281 134 4 5 .0 143 76.9 FBSA < 0 .5 0 0 6.47 6.23 182 110 137 119 1 2 8 [c] 143 [c] 8 .8 7 [c] 1.80 [c] 3 .6 7 [c] 2 .5 0 [c] 3 .9 7 [c] 72.9 [c] 133 [c] 1 1 4 [c] 15.4 [c] 111 [c] 11.4 [c] Avg. SRS Recovery FOSA MeFOSAA EtFOSAA <0.101 0.2 5 8 [b] 0.2 0 0 [d] 4.19 4.00 2.46 8.18 2.12 7.21 0 .3 8 4 <0.253 <0.253 <0.253 <0.253 1.73 3.75 1.75 0.441 [b] 1.83 0 .2 4 3 [a] < 0 .1 0 0 < 0 .1 0 0 < 0.100 [d] 2.22 1.18 1.65 5.07 1.69 5.54 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 1.43 1.85 1.06 < 0 .2 5 0 1.02 < 0 .2 5 0 < 0 .5 0 0 < 0 .5 0 0 < 0.500 [d] 1.73 1.08 1.19 2.42 1.43 2.87 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 1.45 1.76 0 .7 0 3 < 0 .2 5 0 0 .7 5 3 < 0 .2 5 0 13CC4PFOA 83.4% 80.7% 78.8% 83.6% 75.5% 77.6% 78.7% 82.7% 90.8% 96.5% 87.7% 91.2% 85.2% 93.1% 89.4% 89.3% 86.8% 89.7% 92.9% 91.5% 13CC4PFOS 85.4% 87.7% 89.4% [d] 86.6% 82.7% 83.7% 87.5% 85.4% 85.8% 95.7% 84.9% 85.9% 87.4% 90.0% 87.3% 84.5% 89.3% 90.5% 92.8% 92.6% < 0 .2 4 0 <0.252 < 0 .2 5 0 < 0 .1 8 4 [a] < 0 .2 5 0 [c] <0.253 < 0 .2 5 0 < 0 .2 5 0 87.3% 84.8% < 0 .2 4 0 < 0 .2 4 0 <0.252 <0.252 < 0 .2 5 0 < 0 .2 5 0 5.00 175 < 0 .2 5 0 [c] 15.2 [c] <0.253 0 .3 4 8 < 0 .2 5 0 1.16 < 0 .2 5 0 0 .8 9 6 84.5% 88.2% 82.8% 93.6% 11 Table 1. C o n c e n tra tio n R e s u lts fo r T e n n e s s e e R iv e r Fish T is s u e S a m p le s ISO12-01-01-04 Tennessee River Fish Study Fall 2012 SAMPLE ID LIMS ID M SF146 IP F147 IP F148 IP F 1 4 9 IP F151 IP F 1 5 2 IP F 1 5 3 IP F 1 5 5 M SF157 M SF158 IP F 1 5 9 IP F160 IP F 1 6 1 IP F 1 6 2 IP F 1 6 3 IP F164 IP F 1 6 5 IP F166 M SF167 M SF168 M SF169 M SF170 M SF172 M SF173 M SF174 IS O 1 2-0 1-0 1-0 4-1 47 IS O 1 2-0 1-0 1-0 4-1 48 IS O 12-01-01-04-149 IS O 1 2-0 1-0 1-0 4-1 50 IS O 12-01-01-04-151 IS O 12-01-01-04-152 IS O 12-01-01-04-153 IS O 1 2-0 1-0 1-0 4-1 54 IS O 12-01-01-04-155 IS O 1 2-0 1-0 1-0 4-1 56 IS O 1 2-0 1-0 1-0 4-1 57 IS O 1 2-0 1-0 1-0 4-1 58 IS O 12-01-01-04-159 IS O 1 2-0 1-0 1-0 4-1 60 IS O 12-01-01-04-162 IS O 12-01-01-04-163 IS O 1 2-0 1-0 1-0 4-1 64 IS O 12-01-01-04-165 IS O 1 2-0 1-0 1-0 4-1 66 IS O 1 2-0 1-0 1-0 4-1 67 IS O 1 2-0 1-0 1-0 4-1 68 IS O 12-01-01-04-169 IS O 1 2-0 1-0 1-0 4-1 70 IS O 12-01-01-04-171 IS O 12-01-01-04-172 PFBA < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 Average Concentration (ng/g; w et w t.) PFHxA < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .1 0 1 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 0 .3 1 7 [a] < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 < 0 .2 5 2 0 .2 9 4 [a] PFHpA <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 <0.252 PFOA < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .2 4 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 < 0 .4 8 0 0 .6 4 3 < 0 .4 8 0 < 0 .4 8 0 < 0 .2 4 0 PFBS <0.252 <0.252 <0.252 <0.252 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 <0.503 2.21 <0.503 <0.503 <0.252 PFHS < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 0 .7 8 3 0 .3 9 1 < 0 .2 5 0 < 0 .5 0 0 PFOS* 80.3 26.9 69.6 64.2 11.7 29.2 16.3 11.9 115 145 11.2 4 .8 7 8 5 .6 79.3 8 3 .4 283 542 286 50.6 120 435 373 9 6 .4 599 521 FBSA 17.7 [c] 7 2 .8 [c] 65.9 [c] 4 5 .9 [c] 49.5 106 10.1 38.6 74.7 67.3 20.4 8 .4 7 69.2 65.8 4 4 .8 37.5 34.1 33.3 12.1 20.2 67.2 [b] 567 4 5 .8 135 9 5 .0 Avg. SRS Recovery FOSA 0 .4 6 5 7.23 3.64 2.45 1.81 4.92 0 .8 1 6 1.83 1.20 2.28 0 .6 2 0 0 .4 9 0 34.1 31.4 25.6 63.5 [b] 123 88.1 1.28 4.22 7.44 59.9 2.92 9.75 5.20 MeFOSAA EtFOSAA 0 .3 3 6 0 .8 0 6 0 .3 5 1 0 .2 9 9 < 0 .5 0 0 0 .7 3 1 < 0 .5 0 0 < 0 .5 0 0 0 .7 8 6 0 .9 7 4 < 0 .5 0 0 < 0 .5 0 0 3.42 3.09 1.85 3.51 13.2 7.96 < 0 .5 0 0 1.21 3.55 22.5 0 .5 4 5 4 .6 7 1.62 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 0 .8 3 5 0 .8 4 7 < 0 .5 0 0 < 0 .5 0 0 2.66 2.50 1.16 1.00 4 .3 0 3.32 < 0 .5 0 0 1.56 3.01 19.0 0 .6 3 9 3.46 1.43 13CC4PFOA 86.5% 89.8% 91.2% 93.0% 87.7% 85.8% 85.2% 77.2% 91.7% 85.9% 84.1% 83.1% 87.3% 79.3% 81.4% 81.3% 88.6% 85.1% 88.1% 81.2% 77.9% 86.0% 79.7% 89.5% 85.7% 13CC4PFOS 87.2% 90.0% 91.4% 91.5% 83.1% 87.1% 84.6% 85.6% 93.2% 87.9% 85.7% 85.8% 89.7% 80.9% 82.1% 89.4% 89.6% 87.5% 90.6% 81.8% 86.0% 91.6% 80.6% 89.1% 87.7% 12 Table 1. C o n c e n tra tio n R e s u lts fo r T e n n e s s e e R iv e r Fish T is s u e S a m p le s ISO12-01-01-04 Tennessee River Fish Study Fall 2012 SAMPLE ID LIMS ID PFBA Average Concentration (ng/g; w et w t.) PFHxA PFHpA PFOA PFBS PFHS PFOS* FBSA Avg. SRS Recovery FOSA MeFOSAA EtFOSAA 13CC4PFOA 13CC4PFOS IP F 1 7 5 IS 0 1 2 -0 1 -0 1 -0 4 -1 7 3 < 0 .2 5 0 < 0 .2 5 2 <0.252 < 0 .2 4 0 <0.252 < 0 .5 0 0 8 .5 7 36.2 1.36 < 0 .5 0 0 < 0 .5 0 0 88.2% 87.9% IP F176 IS 0 1 2 -01-01-04-174 < 0 .2 5 0 < 0 .2 5 2 <0.252 < 0 .2 4 0 <0.252 < 0 .5 0 0 11.8 28.6 1.54 < 0 .5 0 0 < 0 .5 0 0 86.2% 84.9% IP F177 IS 0 1 2 -0 1 -0 1 -0 4 -1 7 5 < 0 .2 5 0 0 .4 2 1 <0.252 < 0 .2 4 0 <0.252 < 0 .5 0 0 12.7 29.9 1.60 < 0 .5 0 0 < 0 .5 0 0 90.7% 90.5% IP F178 IS 0 1 2 -01-01-04-176 < 0 .2 5 0 < 0 .2 5 2 <0.252 < 0 .2 4 0 0 .3 2 2 < 0 .5 0 0 10.5 51.2 1.23 < 0 .5 0 0 < 0 .5 0 0 85.3% 88.4% IP F 1 7 9 IS 0 1 2 -01-01-04-177 < 0 .2 5 0 < 0 .2 5 2 <0.252 < 0 .2 4 0 0 .5 0 6 < 0 .5 0 0 10.7 35.7 0 .6 7 0 < 0 .5 0 0 < 0 .5 0 0 88.1% 86.5% IP F180 IS 0 1 2 -01-01-04-178 < 0 .2 5 0 0 .2 7 7 <0.252 < 0 .2 4 0 <0.252 < 0 .5 0 0 7.69 27.8 1.43 0 .5 3 9 < 0 .5 0 0 93.8% 89.8% D A L-M S F-LS10001 D A L-IP F-LS10002 IS 0 1 2 -01-01-04-180 < 0 .2 5 0 IS 0 1 2 -0 1 -0 1 -0 4 -1 8 1 < 0 .2 5 0 < 0 .2 5 2 < 0 .2 5 2 <0.252 <0.252 < 0 .2 4 0 < 0 .2 4 0 <0.252 <0.252 < 0 .5 0 0 < 0 .5 0 0 < 0 .3 6 6 3.95 < 0 .2 5 0 < 0 .2 5 0 <0.253 <0.253 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 92.1% 84.3% 91.1% 86.1% D A L-M S F-LS10003 IS 0 1 2 -0 1 -0 1 -0 4 -1 8 2 < 0 .2 5 0 < 0 .2 5 2 <0.252 < 0 .2 4 0 <0.252 < 0 .5 0 0 3.67 < 0 .2 5 0 <0.253 < 0 .5 0 0 < 0 .5 0 0 94.7% 91.4% D A L- IPF-LS10004 D A L- IPF-LS10005 IS 0 1 2 -0 1 -0 1 -0 4 -1 8 3 IS 0 1 2 -01-01-04-184 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 2 < 0 .2 5 2 <0.252 <0.252 < 0 .2 4 0 < 0 .2 4 0 <0.252 <0.252 < 0 .5 0 0 < 0 .5 0 0 < 0 .3 6 6 < 0 .3 6 6 < 0 .2 5 0 < 0 .2 5 0 <0.253 <0.253 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 < 0 .5 0 0 87.3% 87.1% 85.8% 84.3% D A L-M S F-LS10006 IS 0 1 2 -0 1 -0 1 -0 4 -1 8 5 < 0 .2 5 0 < 0 .2 5 2 <0.252 < 0 .2 4 0 <0.252 < 0 .5 0 0 4.31 < 0 .2 5 0 <0.253 < 0 .5 0 0 < 0 .5 0 0 80.4% 85.7% Frequency of Detection (n = 175) 2.9% 12% 4.0% 2.3% 19% 2.9% 98% 87% 82% 49% 45% 100% 100% Average Recovery NA NA NA NA NA NA NA NA NA NA NA 85.2% 88.1% Standard Deviation RSD NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 4.4% 3.4% NA 5.2% 3.8% A n a ly tic a l m e th o d u n c e rta in ty (95% CI) a t + 18% fo r PFBA, + 15% fo r PFHxA, + 2 1 % fo r PFHpA, + 20 % fo r PFOA, + 18% fo r PFBS, + 19% fo r PFHS, + 22% fo r to ta l-P F O S , + 4 0 % fo r FBSA, + 2 0 % fo r FOSA, + 18% fo r M eF O S A A a n d + 19% fo r EtFOSAA NR; S a m p le had LM S p re p a re d a n d re c o v e ry w a s o u ts id e o f 1 0 0 + 50% and re s u lt n o t re p o rte d * ; q u a n tifie d f r o m a lin e a r-P F O S (L-P FO S ) c a lib r a tio n f o r to ta l-P F O S (s u m o f lin e a r a n d b ra n c h e d is o m e r p e a k s ) in s a m p le s , LM S s a n d LCSs. [a ] V a lu e re p o rte d fo r sin g le re p lic a te re s u lts , d u p lic a te re s u lt w a s <LLO Q [b ] RPD > 20% [c] S a m p le had LM S p re p a re d a n d LM S re c o v e ry re s u lt w a s o u ts id e o f 1 0 0 + 30% , b u t w ith in 1 0 0 + 50% and w e re re p o rte d w ith an e x p a n d e d u n c e rta in ty o f + 50% [d ] V a lu e re p o rte d fo r s in g le re s u lt, d u p lic a te s a m p le a n a ly s is had p e a k s h ift o u t o f re te n tio n tim e w in d o w a n d n o re s u lt d e te rm in e d 13 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Table 2. L ab o ra to ry M a trix S p ik e (L M S ) R e c o v e rie s LIM S ID S tu d y ID PFBA PFHxA PFHpA PFOA PFBS PFHS LMS A n a ly te R ecoveries PFOS FBSA FOSA M e F O S A A E tF O S A A IS O 12-01-01-04-001 IS O 12-01-01-04-002 IS O 12-01-01-04-011 IS O 12-01-01-04-022 IS O 12-01-01-04-023 IS O 12-01-01-04-032 T N 11-0194-1/1 Sam ple 1 Pre s tu d y H o m o g e n iza tio n Process C o ntrol MSFOO1 MSF011 IPF022 MSF023 MSF033 IS O 12-01-01-04-043 MSF043 IS O 12-01-01-04-044 MSF044 IS O 12-01-01-04-053 IPF053 IS O 12-01-01-04-064 MSF065 IS O 12-01-01-04-065 IPF066 IS O 12-01-01-04-076 IPF077 IS O 12-01-01-04-086 MSF087 IS O 12-01-01-04-096 IPF098 IS O 12-01-01-04-109 MSF109 IS O 12-01-01-04-118 MSF119 IS O 12-01-01-04-130 MSF131 IS O 12-01-01-04-139 MSF141 IS O 12-01-01-04-151 IPF151 IS O 12-01-01-04-159 IPF161 IS O 12-01-01-04-172 MSF174 Average Recovery Standard Deviation RSD n 107% 109% 101% 111% 109% 102% 111% 139% [a] 92.1% 109% 112% 108% 112% 107% NR 112% 106% 100% 106% 114% 112% 104% 86.0% 84.2% 135% [a] 125% 104% 104% 117% 108% 11% 10% 20 101% 102% 96.8% 106% 100% 122% 100% 96.2% 97.0% 89.6% 102% 101% 95.6% 78.2% 86.2% 103% 107% 98.4% 93.6% 89.3% 98.8% 9.0% 9.1% 21 124% 128% 126% 99.4% 91.8% 101% 92.0% 91.8% 85.0% 89.0% 86.2% 92.0% 88.4% 82.6% 84.0% 106% 104% 103% 93.6% 98.6% 98.4% 13% 14% 21 114% 116% 109% 115% 109% NR 94.0% 90.8% 91.7% 90.8% 91.7% 95.4% 90.6% 84.4% 91.9% 108% 97.5% 107% 108% 97.9% 101% 10% 10% 20 111% NR 95.6% 115% 104% NR 107% 89.7% 87.6% 93.3% 90.0% 94.8% 92.8% 86.2% 89.8% 113% 107% 112% 96.0% 98.4% 99.6% 9.6% 9.7% 19 106% 115% 100% 98.6% 91.8% 102% 140% [a] 91.6% 92.5% 97.2% 95.6% 96.0% 86.6% 84.0% 85.4% 103% 101% 97.2% 100% 103% 99.4% 12% 12% 21 113% [b] 97.5% [b] 103% 96.9 [b]% 105% [b] 102% [b] 94.7% [b] 86.4% 95.1% [b] 80.4% 88.7% 95.7% [b] 99.6% 97.0% [b] 82.5% [b] 97.4% [b ] 96.9% [b] 106% [b] 90.7% [b] 101% [b] 97.2% 8.4% 8.6% 21 53.5% [a, b] 55.4% [a, b] 86.9% [b] 52.2% [a, b] 69.7% [a, b] 53.1% [a, b] 85.9% [b] 90.5% [b] 89.1% [b] 94.6% [b] 104% 93.8% [b] 92.2% 93.1% 79.8% 50.7% [a, b] 64.8% [a, b] 111% [b] 84.0% [b] 85.7% [b] 82.3% 22% 27% 21 95.3% 96.6% 97.6% 97.7% 94.6% 106% 109% 122% 108% 114% 115% 97.2% 97.3% 108% 113% 104% 98.8% 104% 77.0% [b] 99.4% 102% 10% 10% 21 107% 104% 111% 107% 115% 101% 111% 102% 99.0% 88.0% 100% 98.0% 106% 104% 96.6% 103% 105% 97.8% 105% 90.1% 109% 103% 6.6% 6.4% 21 V a lues s h o w n are th o s e d e te rm in e d fo r th e Low -LM S (fo rtifie d a t n o m in a l 5 n g /g ), unless o th e rw is e n o te d fo r th o s e s h o w n fo r high LMS (fo rtifie d a t n o m in a l 125 n g /g ) NR; va lu e w a s o u ts id e o f 100 + 50% and re s u lt n o t re p o rte d fo r th a t sa m p le and LMS re co v e ry e xcluded fro m s u m m a ry sta tistics [a] R e co ve ry o u ts id e o f 100 + 30% , b u t w ith in 100 + 50% and in c lu d e d in s u m m a ry sta tis tic s [b ] V a lue sh o w n w a s o b ta in e d fro m h ig h -le ve l LMS (fo rtifie d a t n o m in a l 2 50 n g /g ); a ccu ra te c o n c e n tra tio n s o f LMSs w e re used fo r re co v e ry ca lcu la tio n s. 105% 98.1% 111% 97.8% 97.7% 88.1% 107% 93.6% 88.5% 88.7% 85.7% 82.4% 93.8% 86.0% 81.2% 82.8% 98.9% 88.9% 102% 99.5% 98.4% 94.1% 8.5% 9.0% 21 3C C4- PFOA 102% 87.1% 83.0% 88.9% 89.3% 84.9% 90.3% 82.9% 83.4% 84.4% 89.9% 86.1% 87.4% 85.6% 82.9% 81.7% 91.9% 94.2% 85.2% 87.6% 83.0% 87.2% 4.8% 5.5% 21 3C C4- PFOS 101% 93.2% 88.6% 93.2% 86.3% 93.0% 88.0% 89.6% 86.3% 89.4% 91.2% 87.0% 87.4% 91.1% 90.5% 79.4% 89.4% 89.0% 87.4% 91.1% 86.6% 89.5% 4.1% 4.5% 21 14 3 Introduction ISO12-01-01-04 Tennessee River Fish Study Fall 2012 A scientific investigation to determ ine the concentrations of select perfluorinated com pounds (P F C ) fish and su rface w a te r from the T e n n e s s e e R iver w a s conducted in the Fall o f 2 0 1 2 , per th e study w o rk plan titled: "R evised W o rk Plan for Fall 2 0 1 2 Fish and S u rface W a te r S am pling in the T e n n e s s e e R iver" W e s to n Solutions, Inc. (W es tc h e s te r, M A ), dated O cto b er 2 0 1 2 . T h e overall purpose o f th e study w a s to provide additional site-specific inform ation on P F C s in fish tissues and surface w ater from within the confines of the W ilson and W h e e le r Reservoirs, T en n essee River, located near Decatur, A lab am a. Laboratory blinded fish tissue sam ples w ere provided for analysis by W eston Solutions, Inc. W eston Solutions, Inc. personnel w ere responsible fo r coordinating all field asp ects of the study such as site p aram eters, fish and w ater collection procedures, handling and storage of collected specim ens, docum entation of individual fish inform ation, ship m en t o f w hole fish for processing, filleting, hom ogenization at a contracted laboratory (TestA m erica-B urlington, V T ) and th e sh ip m en t o f frozen fillets to TestA m erica, and then coordinating shipm ent of tissue hom ogenates to the 3M environmental Laboratory and Axys Laboratories for analysis of P FC s. TestA m erica personnel were responsible for hom ogenizing fillets and sending the tissue hom ogenates to the analytical laboratories for quantitative analysis of P FC s, under the direction of W eston Solutions, Inc. personnel. Prior to the co m m en cem en t of the T en n essee River Fall 2 0 1 2 fish and w ater sampling cam paign, whole largem outh bass and catfish w ere purchased by the 3M Environm ental Laboratory from O sag e Catfisheries (O sage Beach, M O ) as control materials. T h e right side fillet from each o f th ree bass and th ree catfish w e re rem oved in th e laboratory, th e skin w a s rem oved from each fillet, and then control fillet tissue h o m o g en ate w a s prepared and kept at the laboratory. T h e rem aining three bass and three catfish with only left side fillets intact w ere sent to the field, to have left side fillets rem oved as filleting process controls. T h e control fillet tissues kept at the laboratory w ere analyzed prior to the study to establish baseline levels for the field process control m aterials. T h e results from the extraction and L C /M S /M S analysis o f the control tissues m aintained at the laboratory are provided in Table A1 o f Attachment A. W ith the exception of the hom ogenization process controls, this w as a laboratory-blinded study and the true identities of the fish tissues w ere not provided and w ere only identifiable by the anonym ous IDs assigned to the sam ples by W eston Solutions, Inc. personnel. Additionally, W esto n Solutions, Inc. personnel are responsible for associating the determ ined P F C concentrations with the fish collection locations, fish species, age, weight, etc. and overall evaluation of control materials. This report focuses solely on analytical aspects controlled by the laboratory during the analysis of those supplied fish tissues, and describes the extraction procedures and quantitative L C /M S /M S analysis perform ed at the 3M Environmental Laboratory for measuring the target analyte concentrations. Additionally included in this report are the as s e s s m e n t o f the precision and accuracy o f th e d ata based on several quality control (Q C ) param eters, as follows: 1) recovery of fortified target analytes in a control bluegill fish fillet h o m o g en ate matrix as laboratory control s am p les (L C S s) w as assessed as a m easure of intra-batch and inter-batch precision and accuracy; 2) recovery o f fortified target analytes into fish sam p le matrix for ~ 1 0 % o f the sam p les (i.e. prepared for every 10th fish sam p le) as laboratory matrix spikes (L M S s ), and providing a m e a s u re of sam ple-specific target analyte m easurem ent accuracy; and 3) recovery of target analytes from each sam ple w as indirectly determ ined from the recovery of the two stable isotope labeled surrogate recovery standards (S R S s ), with 13C 4-P F O S representative o f th e perfluorinated sulfonates (P F S A S ) and M e F O S A A , E tF O S A A , F O S A and F B S A and 13C 4-P F O A representative o f the perfluorinated carboxylates (P F C A s ). T h e S R S s w ere fortified into each sam ple, LM S and LC S sam ple aliquot prior to extraction. 15 4 Reference Substances ISO12-01 -01 -04 Tennessee River Fish Study Fall 2012 T h e re fe re n c e s u b s ta n c e s used during th e a n a ly s e s a re listed in Table 3, Table 4 and Table 5. M ost w ere purchased from com m ercial vendors and exist as predom inantly linear isom ers. Four of th e referen ce su bstances w e re 3M -sup p lied m aterials, and w ere as follows: P F B S , F B S A , F O S A and technical P FH S ; technical grade m aterials each contain pre-determ ined percentages of linear and branched isom ers. All standard solutions w e re prepared from n eat m aterials and w e re corrected for purity during solution preparations. S e v e ra l referen ce standards are provided as salts, and additional co rrectio n fac to rs fo r salt catio n s (N a+, K+, N H 4+) and protons (H+) w e re also a p p lie d , w h e n applicable. All analyte concentrations w e re d eterm in ed and reported for the anion form o f each. Table 3. Reference Substances: Target Analytes Reference Substance Chemical Name Chemical Formula Identifier Source Expiration Date Storage Conditions Chemical Lot Number TCR Number Physical Description Purity Reference Substance Chemical Name Chemical Formula Identifier Source Expiration Date Storage Conditions Chemical Lot Number TCR Number Physical Description Purity PFBA PFHxA PFHpA Perfluorobutyric acid Perfluorohexanoic acid Perfluoroheptanoic acid C 3F 7C O O H CA S # 375-22-4 Sigm a 4 /2 0 /2 0 1 7 Frozen 11921CH TC R 07-0011 C olorless L iquid 9 7 .6 % PFBS P o ta ssiu m perfluorobutane sulfonate C 5F 11C O O H CAS # 307-24-4 O akw ood 8 /1 8 /2 0 1 8 Frozen C150099B1 TC R -673 C olorless L iquid 9 8 .2 % PFHS Sodium perfluoro-1h e x a n e s u lfo n a te C 6F 13C O O H CAS # 375-85-9 A ldrich 1 2 /2 4 /2 0 1 8 Frozen PU /07219E U TC R -268 C lear C rystals 9 8 .0 % Br-PFOS (linear + branched isomers) P otassium perfluorooctane s u lfo n a te C 4F 9S O 3-K+ CA S # 29420-49-3 3M C 6F 13S O 3N + N /A W ellington L aboratories C 8F 17 S O 3-K+ CA S # 2795-39-3 W ellington L aboratories 1 /1 0 /2 0 1 7 Frozen 101 TC R -121 W hite pow der 9 6 .7 % 3 /2 5 /2 0 1 5 Frozen LPFHxSAM 08 TCR 12-0021 Pow der >98% * 1 2 /1 /2 0 1 4 Frozen br-PFO SK 1111 TC R 12-0026 L iquid > 98% p urity **; 78.8% c o m p o sitio n is lin ear isom er, 2 1 .2 % is b ran ch ed isom ers Technical PFOA (linear + branched) T echnical A m m onium P e rflu o ro o c ta n o ate C 8H F 15O 2 CAS # 95328-99-7 W ellington L aboratories 3 /1 7 /2 0 1 4 Frozen T P F O A 0 3 11 TC R 12-0027 L iquid >97% * FBSA N onafluoro-1butanesulfonam ide C 4 F 9S O 2N H 2 N /A 3M 1/31/2021 Frozen F-11954 TC R -314 Fine W hite C rystals 9 9 .3 % 16 Table 3. Reference Substances: Target Analytes ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Reference Substance Chemical Name Chemical Formula Identifier FOSA P e rflu o ro o c ta n e sulfonam ide C 8F 17 S O 2N H 2 CA S # 754-91-6 MeFOSAA N -m ethylperfluoro- 1 octanesulfonam idoacetic acid C 11H 6F 17N O 4S N /A EtFOSAA N -ethylperfluoro- 1 octanesulfonam idoacetic acid C 12H 8F 17N O 4S N /A Source 3M W ellington L aboratories W ellington L aboratories Expiration Date 3 /2 7 /2 0 1 8 8 /2 6 /2 0 1 3 3 /1 4 /2 0 1 5 Storage Conditions Chemical Lot Number TCR Number Frozen N B #120067-108 TC R -280 Frozen NM eFOSAA0308 TC R 12-0044 Frozen N E tFO SA A 0312 TC R 12-0042 Physical Description W hite pow der L iquid L iquid Purity 98.3% ***; 22.2% branched isom ers >98% * >98% * * ; 100% Purity was assumed to calculate concentrations used in this study and solution concentrations were based on the concentration of the standard material provided by the commercial vendor and assume the vendor adjusted for purity. **; 78.8% Purity (78.8% linear isomer) was used to calculate concentrations of calibration standards, L-PFOS LCS results and CCVs. 100% purity was used to calculated LMS recoveries. ***; Calculated concentrations used in the study using 98.3% purity______________________________________________________________ Table 4. Reference Substances: Stable Isotope Labeled Internal Standards (IS) Reference Substance Chemical Name Chemical Formula Identifier Source Expiration Date Storage Conditions Chemical Lot Number TCR Number Physical Description Purity MPFBA P e rflu o ro -n -[1 ,2 ,3 ,4 13C 4]-b u tan o ic acid 13C 4 H F 7 O 2 IS fo r P F B A W ellington L aboratories 1 0 /3 /2 0 1 5 Frozen M PFC -C -100212 TC R 12-0038 L iquid >98% * MPFHxA P e r f lu o r o - n - [ 1 ,2 - 13C 2]hexanoic acid 13C 212C 4 H F ,,O 2 IS fo r P F H xA W ellington L aboratories 1 0 /3 /2 0 1 5 Frozen M PFC -C -100212 TC R 12-0038 L iquid >98% * MPFHpA P e rflu o ro -n -[13C 4]heptanoic acid 13C 412C 3H F B O2 IS fo r P F H pA W ellington L aboratories 1 0 /3 /2 0 1 5 Frozen M PFC -C -100212 TC R 12-0039 L iquid >98% * M8PFOA P e r f lu o r o - n - [ 13C 8] -o c ta n o ic acid 13C8HF15O2 IS fo r P F O A an d M P F O A W ellington L aboratories 10/3/2015 Frozen M PFC -C -100212 TC R 12-0038 L iquid >98% * 17 Reference Substance Chemical Name Chemical Formula Identifier Source Expiration Date Storage Conditions Chemical Lot Number TCR Number Physical Description Purity Reference Substance Chemical Name Chemical Formula Identifier Source Expiration Date Storage Conditions Chemical Lot Number 18o 2-p f b s [18O 2] -A m m onium P e rflu o ro b u ta n e su lfo n a te C 4 F 9S 18O 2 O "N H 4 + IS fo r PF B S an d F B S A R TI International 3/9/2015 Frozen 11546-107-2 TC R -964, TC R -965 L iquid N /A d3-MeFOSAA N -m ethyl-d3-perfluoro-1octanesulfonam idoacetic acid C 11D 3H 3F 17N O 4 S IS fo r N -M eF O S A A W ellington L aboratories 6 /1 4 /2 0 1 5 Frozen d3NM eFOSAA0408 M3PFHxS Sodium perfluoro-1[ 13C 3] h e x a n e s u lf o n a te 13C 3 12C 3F13SO 3'N a+ IS fo r PFH S W ellington L aboratories 1 0 /3 /2 0 1 5 Frozen M PFC -C -100212 TC R 12-0038 L iquid >98% * d5-EtFOSAA N -ethyl-d5-perfluoro-1octanesulfonam idoacetic acid C 12D 5H 3F 17N O 4 S IS fo r N -E tF O S A A W ellington L aboratories 6 /1 4 /2 0 1 5 Frozen d5N E tFO SA A 0408 ISO12-01 -01 -04 Tennessee River Fish Study Fall 2012 M8PFOS M8FOSA Sodium perfluoro-1[ 13C 8] o c ta n e s u lf o n a te P e r f lu o r o - 1 - [ 13C 8]octanesulfonam ide 13C 8 F n SO 3-N a+ 13C 8 F n SO 2 N H 2 IS fo r PF O S an d M PFO S IS fo r F O S A W ellington L aboratories W ellington L aboratories 1 0 /3 /2 0 1 5 10/3/2015 Frozen Frozen M PFC -C -100212 TC R 12-0038 L iquid M PFC -C -100212 TC R 12-0038 L iquid >98% * >98% * TCR Number TC R 12-0045 TCR 12-0043 Physical Description L iquid L iquid Purity >98% * >98% * _ ______ ______________ * ; 100% Purity was assumed to calculate concentrations used in this study and solution concentrations were based on the concentration of the standard material provided by the commercial vendor and assume the vendor adjusted for purity.__________________________________ 18 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Table 5. Reference Substances: Stable Isotope Labeled Surrogate Recovery Standards (SRS) Reference Substance 13C 4-PFO A 13C 4-P FO S Chemical Name Chemical Formula Purpose/Use Source Expiration Date Storage Conditions Chemical Lot Number Perfluoro-n-[1,2,3,4-13C4]-octanoic acid 13C412C4HF15O2 Surrogate for PFCAs Wellington Laboratories 12/7/2013 Frozen MPFOA1210 Sodium perfluoro-1-[1,2,3,4-13C4]octanesulfonate 13C412C4F17SO3-Na+ Surrogate for PFSAs, MeFOSAA, EtFOSAA, FOSA and FBSA Wellington Laboratories 12/21/2014 Frozen MPFOS1211 TCR Number TCR11-0001 TCR12-0003 Physical Description Liquid Liquid Purity >98% * >98% * *; 100% purity was used to calculate concentrations used in this study and based on the concentration of the standard material provided by the commercial vendor. 5 Method Summary 5.1 Methods T h e re-hom ogenization of fish tissue sam ples, and the sam ple extractions and quantitative L C /M S /M S analyses w ere perform ed following validated 3M Environm ental Laboratory method E T S -8 -0 4 5 .1 [M alinsky et. al. 2 0 1 1 , A n a ly tic s C h e m ic a A c ta 683:p p 248-257]. T h e bulk o f the fish tissue sam ple analyses occurred from D ec e m b er 27, 2 0 1 2 through February 6, 2 0 1 3 , with the exception o f the original analysis for the fillet process controls and the first hom ogenization process controls which occurred in N o vem b er, 2 0 1 2 . T h e identities o f the instrum ents used for collection o f the L C /M S /M S d a ta are listed in Table 6. T h e analytical batches used for reporting th e results o f the fish analyses are listed in Table 10. 5.2 Sample Receipt All sam ples w ere received frozen on dry ice and w ere shipped for overnight delivery from T estA m erica (Burlington, V T ) to 3M by FedE x. Upon receipt at 3M , that sam ple w as entered into the 3M Environm ental Laboratory L IM S system as sam ple ID IS 0 1 2 -0 1 -0 1 -0 4 -0 0 1 A single hom ogenization process control fish tissue sam ple w as received from T estA m erica on N o vem b er 22, 2011 with appropriate chain o f custody, logged into L IM S as IS 0 1 2 -0 1 -0 1 -0 4 001 and analyzed on D ecem ber 6, 2012. T h e rem ainder o f control fish tissues (9), T e n n e s s e e R iver fish fillet tissues (1 6 5 ) and aqueous fillet equipm ent rinseates (10) w e re received from T estA m erica on two dates: D e c e m b er 27, 2 0 1 2 and January 3, 2 0 1 3 and w ere logged into the s a m e L IM S project as IS 0 1 2 -0 1 -0 1 -0 4 002 through -185. C op ies o f all chains o f custody for the s am p les received w e re retained within th e study folder and electronically scanned into the 3M Environm ental Laboratory L IM S system . D ata for this study is associated with study IS 0 1 2 -0 1 -0 1 -0 4 . 19 5.3 Sample Preparation/Extraction ISO12-01-01-04 Tennessee River Fish Study Fall 2012 For the frozen fish tissue hom ogenates received for this study, each w as thaw ed ~ 3 0 minutes and then rem oved from its s a m p le bag and re-ho m o g enized with dry ice using a R ob o t-C o u p e m odel R SI 2Y-1 stainless steel tissue hom ogenizer, then transferred back into the s am e sa m p le baggie and left overnight in a fre e ze r to allow th e dry ice to dissipate. F o r preparative extraction of each analytical sam ple, a nom inal 0 .5 gram aliquot of re-hom ogenized fish tissue sam ple w as accurately w eighed into a centrifuge tube. Each fish sam ple w as typically aliquoted in duplicate with one being th e prim ary analytical s a m p le and the second being a duplicate analytical sam ple. A stable isotope labeled P F C internal standard (IS) and stable isotope labeled surrogate recovery standard (S R S ) mixture w as added to each sam ple aliquot, after which 5.0 m L of acetonitrile w as added. For LM Ss, a target analyte mixture w as also added. T h e sam ple w as mixed and then placed into a freezer for g reater than 1 hr to facilitate precipitation o f proteins and fats. S am p les w ere then centrifuged at greater than 1 0 ,0 0 0 x g to pellet the solids. Then, a 1.00 m L aliquot of supernatant extract w as transferred to an autosam pler vial with 0 .0 1 0 m L form ic acid to m ake the final analyzed extract. Typically, approxim ately tw enty fish sam p le s w e re prepared and extracted, each sa m p le in duplicate, and analyzed as part of each analytical batch. Each analytical batch w as also prepared with the following Q C elem ents: (1) each sam ple and L M S aliquot received two S R S s (13C 4-P F O A and 13C 4-P F O S ) and ISs prior to extraction, for evaluation o f overall d ata precision for P F C carboxylates and P F C sulfonates/sulfonam ides, respectively; (2) for approxim ately every tenth fish sam ple extracted, two additional aliquots w e re prepared as LM S s and fortified with known quantities of target analytes, ISs and SR S s; (3) a set of sixteen solvent calibration standards w e re prepared in acetonitrile; (4) a set o f L C S s w a s prepared by fortifying known quantities o f targ et analytes, ISs and S R S s into a control bluegill fillet tissue h o m o g en ate at th ree levels, each level in triplicate; (5) matrix blanks (m ethod blanks) w e re nom inal 0.5 g o f control bluegill fillet matrix and prepared with and without IS and S R S and used to evalu ate en do g en ou s levels of analyte in th e L C S matrix; and (6) solvent blanks w ere prepared with the acetonitrile used to m ake calibration standards and prepared with and without ISs and SRSs. 5.4 LC/MS/MS Analysis A nalyses w ere perform ed following a previously validated and published L C /M S /M S m ethod [M a lin s k y e t. al. 2 0 1 1 , A n a ly tic s C h e m ic a A c t a 683:p p 2 4 8 -2 5 7 ], d e s c rib e d in 3 M E n v iro n m e n ta l Laboratory m ethod E T S -8 -0 4 5 .1 . T h e relative percent difference (R P D ) of the duplicate analytical sam ple results w as used as a m easure of the sam ple-specific analytical precision. T h e d eterm ined recoveries o f fortified targ et analytes from L M S sam p les w ere used as a m e a s u re o f s a m p le -s p e c ific an d s a m p le m atrix a n alytical a c c u ra c y . R e c o v e ry o f th e S R S s ( 13C 4P F O S an d 13C 4-P F O A ) fro m e a c h a n alytical s a m p le w a s ad d itio n ally u sed as a s e c o n d a ry m easure of the target analyte accuracy for each sam ple. D etails o f th e specific instrum ent p aram eters, the liquid chrom atography gradient program , and th e s p e c ific m a s s tran sitio n s a n a ly ze d a re d e ta ile d in th e ra w d a ta , an d a re briefly d e s c rib e d b elo w in Table 6, Table 7, Table 8 and Table 9. A dditionally, th e analytical b atc h e s conducted fo r collection o f L C /M S /M S d a ta a s s o c ia te d w ith this study a re listed in Table10. 20 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Table 6. LC/MS/MS Instrument Information (ETS-8-045) Instrum ent N am e(s) A nalytical M ethod ID Liquid C hrom atograph G uard colum n (instrum ent cleanup) Injection V olum e M ass Spectrom eter Ion S ource Polarity S o ftw a re C7and C8 PFCAs, PFSAs and SRSs Extraction column Analytical colum n C olum n Switching V alve C4 and C6 PFCAs Analytical colum n B eethoven (B e), G in ger (g), Jonas (j) and M aryA nn (m ), E TS -8-045.1 Agilent 1200 or 1290 H P L C System Placed betw een Purge V alve and A utosam pler; Prism RP (2.1 x 5 0 m m , 5 mm) 2 0 mL , 4 0 mL or 5 0 mL A PI 5 0 0 0 Triple Q uadrapole or A PI 4 5 0 0 Triple Q uad. Turbolon Spray (Electrospray) N egative Ion A nalyst 1.6.1 W aters Corp. O asis HLB Online Colum n (3 x 20 m m , 25mm); 30C B etasil C 1 8 (2.1 x 1 0 0 m m , 5 mm); 30C 0 to 5 min (w aste); 5 m inutes to 21 m inutes to analytical co lu m n P rism R P (2.1 x 5 0 m m , 5 mm); 3 0 *0 Table 7. Gradient Liquid Chromatography Conditions (ETS-8-045); C7-C12PFCAs, PFSAs and SRSs Step Num ber Total Tim e [m in ] Flow Rate [m L/m in] 0 0 .0 0 0 .4 0 0 1 3 .0 0 0 .4 0 0 2 3 .5 0 0 .4 0 0 3 13.5 0 .4 0 0 4 15.5 0 .4 0 0 5 16.0 0 .4 0 0 6 18.0 0 .4 0 0 7 18.3 0 .4 0 0 8 2 1 .0 0 .4 0 0 Percent M obile Phase A [2 m m A queous A m m onium A cetate] 9 7 .0 9 7 .0 7 0 .0 4 0 .0 4 0 .0 10.0 10.0 9 7 .0 9 7 .0 Percent M obile Phase B [ A cetonitrile] 3.0 3.0 3 0 .0 6 0 .0 6 0 .0 9 0 .0 9 0 .0 3.0 3.0 21 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Table 8. Gradient Liquid Chromatography Conditions (ETS-8-045); for C4-C6PFCAs S te p Num ber 0 1 2 3 4 5 6 T otal Tim e [m in ] 0 .0 0 3 .0 0 3 .5 0 9 .0 0 15.0 15.1 19.0 F lo w R ate [m L /m in] 0 .3 0 0 0 .3 0 0 0 .3 0 0 0 .3 0 0 0 .3 0 0 0 .3 0 0 0 .3 0 0 P erce n t M obile P h ase A [5 m m A q u eo us A m m onium A cetate w ith 0.1% A c e tic A c id ] 9 0 .0 9 0 .0 3 0 .0 5.0 5.0 9 0 .0 9 0 .0 P erce n t M obile P h ase B [ M eth an o l] 10.0 10.0 7 0 .0 9 5 .0 9 5 .0 10.0 10.0 Table 9. Analytes, Surrogates, Internal Standards, and Transitions Compound A n alyte Description P FB A (C 4 Acid) P F H x A (C 6 Acid) P F H p A (C 7 Acid) Target Target Target P F O A (C 8 Acid) Target P FB S (C 4 Sulfonate) P F H S (C 6 Sulfonate) Target Target P FO S (C 8 Sulfonate) FBSA FOSA M eFO SA A EtFO SAA Target Target Target Target Target Internal Standards (IS) 22 M R M Transition(s) 213>169 313>269 3 1 3 > 119 363>319 363>169 413>369 413>219 413>169 299>80 299>99 399>80 399>99 499>80 499>99 499>130 298>78 498>78 570>83 570>169 570>219 584>83 584>169 584>219 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Table 9. Analytes, Surrogates, Internal Standards, and Transitions Compound A n alyte Description M R M Transition(s) [1 ,2 ,3 ,4 - 13C ]-P F B A IS for P FB A 217>172 [1 ,2 - 13C 2 ]-P F H x A IS for P FH xA and P FH p A 315>270 [ 1 ,2 ,3 ,4 ,5 ,6 ,7 ,8 -13C 8]-P F O A [18O 2]-P F B S [13C 3]-P F H S [ 1 ,2 ,3 ,4 ,5 ,6 ,7 ,8 -13C 8 ]-P F O S IS fo r P F O A an d [1 ,2 ,3 ,4 -13C 4]-P F O A IS for P F B S and F B S A IS for P FH S IS fo r P F O S an d [1 ,2 ,3 ,4 -13C 4 ]-P F O S 421>376 303>84 402>80 507>80 [ 1 ,2 ,3 ,4 ,5 ,6 ,7 ,8 -13C 8 ]-F O S A d3-M eFO SA A IS for F O S A IS for M e F O S A A d 5-E tF O S A A IS for Et F O S A A Surrogate Recovery Standards (SRS) 506>78 573>83 573>169 573>219 589>83 589>169 589>219 [1 ,2 ,3 ,4 -13C 4]-P F O A Surrogate for PFC A s 417>372 [1 ,2 ,3 ,4 -13C 4 ]-P F O S Surrogate for P FS A s, E tFO S A A , MeFOSAA, FOSA & FBSA 503>80 M u ltip le M S /M S ion tran sitio n s a re s u m m e d w h e n m o re th an o n e transitio n is listed fo r th e a n a ly te . Table 10. Analytical Batches A nalytical Batch # 1 2 3 4 5 6 7 8 9 10 11 A nalytical Batch ID j1 2 1 2 0 6 a j1 3 0 1 1 8 a j1 3 0 1 2 1 a j1 3 0 1 2 3 a g130124a j1 3 0 1 2 5 a j1 3 0 1 2 8 a g130128a m 130204a g130204a m 121206a A n a ly te s R e p o r t e d IaI PFBA, PFHxA PFBA, PFHxA PFBA, PFHxA PFBA, PFHxA PFBA, PFHxA PFBA, PFHxA PFBA, PFHxA PFBA, PFHxA PFBA, PFHxA PFBA, PFHxA P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S Sam ples A nalyzed IS O 1 2 -0 1 -0 1 -0 4 -0 0 1 IS O 1 2 -0 1 -0 1 -0 4 -0 0 2 to -0 2 2 IS O 1 2 -0 1 -0 1 -0 4 -0 2 3 to -0 4 3 IS O 1 2 -0 1 -0 1 -0 4 -0 4 4 to -0 6 4 IS O 1 2 -0 1 -0 1 -0 4 -0 6 5 to -0 8 5 IS O 1 2 -0 1 -0 1 -0 4 -0 8 6 to -1 0 8 IS O 1 2 -0 1 -0 1 -0 4 -1 0 9 to -1 2 9 IS O 1 2 -0 1 -0 1 -0 4 -1 3 0 to -1 5 0 IS O 1 2 -0 1 -0 1 -0 4 -1 5 1 to -1 7 1 IS O 1 2 -0 1 -0 1 -0 4 -1 7 2 to -1 8 5 IS O 1 2 -0 1 -0 1 -0 4 -0 0 1 23 Table 10. Analytical Batches ISO12-01 -01 -04 Tennessee River Fish Study Fall 2012 A nalytical Batch # A nalytical Batch ID A n a ly te s R e p o r t e d IaI Sam ples A nalyzed P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, 12 m 1 3 0 1 2 2 b IS O 1 2 -0 1 -0 1 -0 4 -0 0 2 to -0 2 2 M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, 13 m 1 3 0 1 2 9 b IS O 1 2 -0 1 -0 1 -0 4 -0 2 3 to -0 4 3 M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, 14 B e130121b IS O 1 2 -0 1 -0 1 -0 4 -0 4 4 to -0 6 4 M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, 15 B e 1 3 0 1 2 3 b IS O 1 2 -0 1 -0 1 -0 4 -0 6 5 to -0 8 5 M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, 16 B e 130128 a IS O 1 2 -0 1 -0 1 -0 4 -0 8 6 to -1 0 8 M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, 17 B e 130130 a IS O 1 2 -0 1 -0 1 -0 4 -1 0 9 to -1 2 9 M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, 1 8 j1 3 0 2 0 1 a IS O 1 2 -0 1 -0 1 -0 4 -1 3 0 to -1 5 0 M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, 19 g 1 3 0 1 3 0 b IS O 1 2 -0 1 -0 1 -0 4 -1 5 1 to -1 7 1 M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S P F H pA , PFO A, PFBS, PFHS, PFOS, FBSA, FO SA, 20 g130201a IS O 1 2 -0 1 -0 1 -0 4 -1 7 2 to -1 8 5 M e F O S A A , E t F O S A A , 13C 4- P F O A a n d 13C 4- P F O S [a ] E a c h e x tr a c t w a s a n a ly z e d in o n e a n a ly tic a l r u n f o r s h o r t c h a in a c id s (P F B A a n d P F H x A ) a n d th e n re -a n a ly z e d in a s e c o n d a n a ly tic a l ru n f o r P F H p A , P F O A , PFBS, PFH S, PFO S, FB SA, FO S A , M e F O S A A a n d E tF O S A A 6 Results All reported concentration results and recovery v alu es are rounded to th re e significant figures. Statistical valu es (R S D s , R P D s, etc.) are rounded to two significant figures. All results w ere c a lc u la te d using full precision ra w d a ta during d a ta c alcu latio n s in M ic ro s o ft E x c e lTM , an d as a result so m e valu es m ay vary slightly w h en th e rounded values reported herein are carried through the s am e calculations. 6.1 Calibration Calibration standards w ere prepared by spiking varying known quantities of target analytes, S R S s and ISs into acetonitrile. A set o f 16 acetonitrile solvent calibration standards w ere prepared with each analytical batch, ranging from nominal 0 .0 0 2 5 0 ng/m L to 2 5 0 ng/m L for targ et analytes and S R S s ; equivalent to nom inal 0 .0 2 5 0 ng/g to 2 5 0 0 ng/g in fish tissue after adjustm ent for dilution during extraction. T h e s a m e am ount o f ISs w ere added as w ere added to each fish sam ple during extraction. Following the analysis of calibration standards, the nominal concentration of each standard w as plotted versus the m easured analyte p eak area/IS p e a k a re a response ratio. A quadratic equation with 1/x w eighting w a s used to fit the calibration response data. Calibration curves w ere not forced through zero. Calculating the determ ined standard concentration using the resultant calibration curve and com paring to the known concentration confirm ed accuracy of each curve point within 100 2 5 % (+ 30 % at the LLO Q ). T h e correlation coefficient (r) w a s g re a te r than 0 .9 9 9 , and coefficient o f determ ination (r2) w as greater than 0 .9 9 0 . High and/or low calibration standards w ere excluded as needed to m eet 24 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 L LO Q requirem ents and to focus quantitation at high end or low end o f the curve, as needed based on sa m p le concentrations in th e batch. For P F O S , th e calibration standards w e re prepared with b rP F O S , which is com posed o f a well characterized isom er mixture com posed of 78 .8 % (w/w) linear P F O S isom er and 21 .2 % (w/w) of branched isom ers. B ecause fish sam ples show ed a predom inance of linear isom er, P F O S quantitation w a s based on th e chrom atographically distinct L -P F O S p e a k in calibration standards and those results used to construct a L -P F O S calibration curve. Sam ples, LM Ss and L C S s w ere quantified by integration of total P F O S (linear + branched isom ers) using the LP F O S calibration. For LCSs, both L -P F O S and total-P FO S (branched + linear isomers) recoveries w e re determ ined and are reported in Table 11 o f section 6 .5. 6.2 Limits of Quantitation (LOQs) T h e low er limit o f quantitation (L L O Q ) w a s typically the lowest non-zero calibration standard in the curve that m et linearity and accuracy requirem ents (1 0 0 + 3 0 % ) and for which the peak area counts w ere at least 2 X that of the average analyte peak area counts determ ined from the acetonitrile solvent blanks. T h e L L O Q for all analytes in this study w a s the typically nom inal 0 .2 5 0 ng/g fish concentration, equivalent to the 0 .0 2 5 0 ng/m L standard, with som e exceptions as noted in th e raw d ata. R esults in Table 1 indicate th e nom inal L L O Q values w hen results w ere below the LLO Q . T h e U L O Q w as typically the nom inal 2 5 0 ng/m L standard (equivalent to 2 5 0 0 ng/g in fish tissue), with s o m e exceptions as noted in the raw data. 6.3 Continuing Calibration During the course of analysis of each analytical batch, continuing calibration verifications (C C V s ) w ere perform ed by regular injection of mid-level calibration standards throughout the analysis. T h e back calculated concentration results of C C V s w ere used to verify that the instrum ent response and th e initial calibration curve w e re still in control. All C C V s for all analytes w ere within 100+ 2 5 % and m et m ethod criteria, with the following exceptions: P F H p A :m 130122b 052 (993% ), m 1 3 0 1 2 2 b 0 9 8 (NA, outside of curve range), m 1 3 0 1 2 2 b 1 1 0 (497% ), m 1 3 0 1 2 2 b 1 2 2 (72.1% ), m 1 3 0 1 2 2 b 1 2 8 (NA, outside of curve range). PFOA: m 130122b052 (559% ), m 130122b110 (383% ), m 130122b122 (134% ) and m 1 3 0 1 2 2 b 1 2 8 (NA, outside of curve range). PFBS: m 130122b052 (425% ), m 130122b110 (126% ), m 130122b122 (2.02% ) and m 130131a005 (130% ). P F H S :m 1 3 0 1 2 2 b 0 5 2 (NA, outside of curve range) and m 1 3 0 1 2 2 b 1 2 8 (57.6% ) P FO Sm 130122b052 (529% ), m 130122b110 (4050% ), j130201a074 (74.3% ) and j130201a112 (71.4% ) FBSA: m 130122b052 (402% ), m 130122b110 (304% ), m 130122b128 (61.6% ), m 130129b052 (70.5% ), m 130131a005 (70.5% ), j130201a041 (134% ), j130201a074 (128% ) and j130201a112 (126% ). FOSA: m 130122b052 (392% ) and m 130122b110 (31.7% ). M eFO SAA: m 130122b052 (160% ) and m 130122b110 (48.5% ) EtFOSAA: m 130122b052 (38.8% ) and m 130122b110 (18.5% ) 13C 4-P F O A (S R S ): m 1 3 0 1 2 2 b 0 5 2 (3 2 .3 % ), m 1 3 0 1 2 2 b 0 9 8 (4 .0 6 % ), m 1 3 0 1 2 2 b 1 1 0 (4 1 .6 % ) and m 130122b128 (11.4% ) 13C 4-P F O S (S R S ): m 1 3 0 1 2 2 b 0 5 2 (1 6 .2 % ), m 1 3 0 1 2 2 b 0 9 8 (4 .0 6 % ), m 1 3 0 1 2 2 b 1 1 0 (3 7 .0 % ) and m 130122b128 (4.07% ) 25 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 W h e n C C V s failed to m eet criteria, the analytical run w as evaluated m ore closely for LM S recoveries and LC S results, and a potential cause of C C V recovery failures w as evaluated and d ocu m en ted with the batch paperw ork. For exam p le, in analytical run m 1 3 0 1 2 2 b the standard solution used for several C C V injections ran low and w e re not effectively injected, resulting in C C V valu es well outside o f the 1 0 0 + 2 5 % criterion. In all instances, L M S results and L C S results w ere additionally evaluated to provide justification for reporting the results from those batches. All C C V failures w e re d o cu m en ted into a m ethod deviation and included in th e raw data. 6.4 Blanks T h re e types o f blanks w ere analyzed with fish sam ples: (1) acetonitrile solvent blanks w ere typically analyzed before or after calibration standards and C C V s for evaluating and abating any potential carry over and at tim es are used in conjunction with m ethod blanks to help assign LLO Q s; (2) fish matrix blanks w e re prepared with nom inal 0 .5 g o f a control bluegill fillet hom ogenate T N 1 1 -0 1 94 -1 /1 and w ere spiked with and without ISs and S R S s and used to provide endogenous background levels for the fish matrix used to prepare LCSs; and (3) process control blanks used to evaluate the hom ogenization process at TestA m erica (control sunfish tissue T N 1 1 -0 1 9 4 -1 /1 ) and th e process controls for th e field filleting process (B ass,T N 12-0365-1/13, -2/13 and -3 /1 3 and Catfish T N 1 2 -0 3 65 -1 /1 3 , -2 /1 3 and -3/13). T h e fillet process controls w e re 3 largem outh w hole bass (T N 1 2 -0 3 6 4 -1 /1 3 , -2 /1 3 and -3 /1 3 ) and 3 w hole catfish (T N 1 2 -0 3 6 5 -1 /1 3 , -2 /1 3 and -3 /1 3 ) for which the right side fillets w ere rem oved at the 3M Environm ental Laboratory, hom ogenized and analyzed prior to fish sam ple receipt for the study. T h o s e fish, with right side fillets rem oved , w e re sen t to the field and left side fillets rem oved in the field during fish filleting process. T h o s e process control sam p les w ere returned blinded to the laboratory regarding their true identities. H om ogenization process controls w ere returned and w ere identifiable to the laboratory based on identities placed on th e m by T estA m erica. Final results and fish identities for process controls will be verified in the W esto n Solutions, Inc. final study report. All blank results w ere carefully evaluated for issues such as background instrum ent interferences, analytical carry over during the analysis to provide a background level with which to use for assigning L L O Q s , and to d eterm in e en do g en ou s valu es o f each analyte in th e fish matrix used for preparation o f L C S s. All o f the blank sam ple results w ere considered acceptable upon evaluation of the raw data based on the intended use of the different types of blanks. T h e sunfish control matrix T N 1 1 -0 1 94 -1 /1 w as the matrix used for LC S preparations, and w as the s am e matrix used for the independent evaluation of the hom ogenization process (hom ogenization process controls) at TestA m erica. T h at matrix has low m easurable levels of endogenous total P F O S , and other analytes, and those values subtracted from LC S results during recovery calculations. T h e results o f all sam p les, including hom ogenization and filleting process controls are included in Table 1. 26 6.5 Laboratory Control Samples (LCSs) ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Laboratory control sam ples (LC S s) w ere prepared for each analytical batch by fortifying known quantities o f the target analytes, ISs and S R S s into accurately w eighed (nom inal 0.5 gram ) aliquots o f a control bluegill fillet tissue prior to extraction then carrying the LC S through the extraction and analysis procedure. T h e LC S sets w ere prepared at three levels of nominal 1.0, 10 and 1 0 0 ng/g (w w ), and each level w a s prepared in triplicate. T h e P F O S used to fortify LCSs w as b rP FO S purchased from W ellington Laboratories and with a known 78.8% (w/w) linear isom er composition and 2 2 .2 % (w /w ) branched isom er composition. B ecause calibration w as perform ed using only the chrom atographically separated L -P F O S isom er peak, both LP F O S and total-P FO S recoveries from LCSs w ere determ ined. T otal-P F O S w as always determ ined from sam ples, LM S s and matrix blanks. T h e LC S results w ere used to determ ine the analytical m ethod accuracy (average recovery) and precision (R S D ). T h e LC S accuracy and precision results, and the analytical method uncertainties at the 95% confidence interval (95% CI; 2-tim es standard deviation), determ ined from th e statistical evaluation o f L C S results are show n in Table 11. T h e m ethod accep tan ce criteria o f 1 0 0 3 0 % w a s typically m et, with s o m e exceptions as noted in the T a b le . T h e average low -LCS, m id-LC Ss and high-LCSs recoveries over 10 analytical batches, and overall for all L C S s levels, w a s within 1 0 0 + 14 % for all analytes and S R S s . T h e LCS recoveries w ere calculated using the following equation: TLC_S_,,Recovery = -(-D--e-t-e-r-m---in--e-d---C--o-n--c-e-n--t-r-a-t-i-o-n--o--f--L--C--S----E--n--d-o--g-e--n-o--u-s--M---a-t-r-i-x--C--o-n--c-.--*100% Spike Concentration 27 Table 11. LCS Results LCS ID PFBA PFHx A PFHpA PFOA PFBS ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Analyte Recovery (%) PFHS TotalPFOS [b] L-PFOS [b] FBSA FOSA MeFOSAA EtFOSAA 13C4-PFOA 13C4-PFOS Analytical Batch ID LCS L o w -1 ra LO O rsi LCS L o w -2 rsi LCS L o w -3 a cra < * LCS M id - 1 X X CL LL LCS M id - 2 CL < C LL LCS M id - 3 CL ra LO O LCS H ig h -1 rsi rsi LCS H ig h -2 LCS H ig h -3 LCS L o w -1 _Q rsi rsi LCS L o w -2 o m LCS L o w -3 a cra LCS M id - 1 < X X CL LL LCS M id - 2 CL < C LCS M id - 3 LL CL ra 0 0 LCS H ig h -1 o m LCS H ig h -2 LCS H ig h -3 115% 101% 105% 111% 111% 106% 109% 103% 108% 106% 107% 103% 105% 111% 103% NA 108% 102% 100% 110% 109% 108% 108% 104% 106% 96.5% 96.5% 93.8% 104% 105% 98.8% NA 84.4% 69.9% 72.5% 105% 107% 106% 87.5% 89.6% 88.7% 126% 125% 119% 106% 112% 105% NA NA NA NA NA NA NA 74.7% 63.9% 66.3% 110% 115% 111% 102% 100% 101% 109% 116% 110% 104% 106% 101% NA NA NA 108% 99.2% 104% 109% 110% 107% NA NA NA 104% 104% 98.6% 97.9% 101% 96.8% 83.6% 70.0% 6 6 .8 % [a] 106% 97.1% 98.9% 105% 105% 102% 103% 98.6% 101% 99.3% 102% 96.8% 99.0% 102% 96.2% 85.9% 78.0% 79.1% 118% 111% 117% 114% 116% 113% 115% 110% 112% 110% 117% 110% 111% 116% 110% NA NA NA 105% 96.6% 105% 111% 112% 110% 108% 104% 106% 108% 115% 108% 105% 111% 105% 6 7 .5 % [a] 6 7 .7 % [a] 6 9 .3 % [a] 123% 117% 120% 133% 135% 132% 126% 117% 123% 90.3% 91.8% 87.6% 78.1% 80.3% 76.6% 67.0% 94.6% 90.6% 91.1% 93.4% 94.7% 91.3% 96.1% 91.2% 95.7% 90.6% 95.8% 89.5% 96.2% 104% 99.2% NA 51.7% NA 49.1% NA 119% 102% 103% 110% 110% 103% 108% 105% 108% 98.4% 103% 98.7% 99.7% 108% 99.0% 87.1% 86.1% 89.1% 115% 100% 101% 104% 108% 104% 107% 103% 104% 92.1% 97.6% 92.7% 91.8% 100% 93.8% 83.3% 79.0% 84.2% 112% 100% 102% 106% 110% 108% 104% 104% 102% 87.4% 89.6% 86.9% 85.5% 87.2% 87.7% 83.3% 82.8% 87.2% 109% 101% 101% 104% 106% 104% 102% 101% 100% 88.6% 90.8% 88.2% 89.6% 88.4% 93.3% 88.0% 88.9% 92.7% 28 Table 11. LCS Results Analytical Batch ID LCS ID LCS L o w -1 _Q cn rsi LCS L o w -2 o m LCS L o w -3 a cra < * LCS M id - 1 X X CL LL LCS M id - 2 CL < C LL LCS M id - 3 CL ra rsi LCS H ig h -1 o m LCS H ig h -2 LCS H ig h -3 LCS L o w -1 _a rsi LCS L o w -2 o m ai C LCS L o w -3 a cra LCS M id - 1 <" X X CL LL LCS M id - 2 CL < C LL LCS M id - 3 CL ra m rsi LCS H ig h -1 o m LCS H ig h -2 LCS H ig h -3 PFBA PFHx A PFHpA PFOA 106% 97.5% 92.3% 102% 94.7% 87.9% 105% 97.4% 92.5% 105% 101% 84.3% 103% 100% 82.7% 101% 99.8% 84.9% NA NA NA NA NA NA NA NA NA 105% 96.0% 82.3% 105% 95.4% 88.4% 103% 95.4% 84.4% 109% 107% 97.7% 109% 104% 95.9% 98.2% 94.5% 83.5% NA NA 86.1% NA NA 83.2% NA NA 87.7% 106% 104% 107% 104% 99.7% 98.8% 100% 98.1% 97.5% 95.1% 93.6% 95.3% 101% 97.1% 88.5% 83.9% 79.3% 83.8% ISO12-01-01-04 Tennessee River Fish Study Fall 2012 PFBS Analyte Recovery (%) PFHS TotalPFOS [b] L-PFOS [b] FBSA FOSA MeFOSAA EtFOSAA 13C4-PFOA 13C4-PFOS 105% 101% 104% 107% 107% 104% 90.3% 94.7% 89.7% 87.3% 90.4% 91.2% 93.8% 96.7% 85.2% 87.6% 87.4% 90.5% 85.0% 81.2% 83.9% 90.2% 90.2% 87.3% 80.7% 71.6% 6 9 .4 % [a] 93.6% 89.2% 91.4% 103% 102% 91.4% 83.7% 81.3% 83.7% 101% 95.6% 102% 100% 97.4% 97.8% 115% 104% 103% 89.6% 87.1% 88.0% 98.1% 94.3% 84.9% 97.6% 97.6% 101% 99.2% 94.0% 99.8% 97.4% 94.3% 94.9% 76.0% 70.6% 6 9 .2 % [a] 89.2% 86.8% 88.0% 98.1% 94.2% 85.3% 90.0% 89.0% 92.3% 75.4% 93.4% 72.5% 90.2% 71.3% 68.8% [a] 67.4% [a] 68.4% [a] 75.4% 61.6% [a] 51.1% [a] 128% 93.6% 95.9% 94.8% 95.0% NA NA NA 104% 1 3 4 % [a] 106% 129% 107% 119% 108% 119% 113% 110% 65.7% [a] 66.6% [a] 67.6% [a] 104% 86.5% 94.0% 93.6% 99.3% 93.9% 103% 97.6% 95.5% 96.8% 89.5% 86.1% 84.2% 97.0% 98.6% 99.6% 101% 101% 93.5% 90.3% 82.8% 88.8% 93.4% 90.8% 92.2% 90.1% 86.3% 87.4% 82.0% 80.4% 81.8% 88.6% 93.7% 89.9% 97.2% 92.7% 85.5% 79.5% 78.2% 78.8% 86.8% 84.2% 87.3% 87.9% 86.5% 105% 84.7% 86.0% 86.4% 86.4% 87.8% 85.6% 92.8% 86.9% 81.7% 85.1% 80.8% 87.0% 89.8% 82.9% 89.2% 85.7% 86.0% 104% 89.7% 88.3% 86.5% 88.0% 90.5% 84.1% 97.5% 89.3% 85.8% 89.1% 87.5% 91.7% 29 Table 11. LCS Results Analytical Batch ID LCS ID LCS L o w -1 _Q m CM LCS L o w -2 O m CL) C LCS L o w -3 a cra LCS M id - 1 <" X X CL LL LCS M id - 2 CL < C LL LCS M id - 3 CL ra rsi LCS H ig h -1 o m bo LCS H ig h -2 LCS H ig h -3 LCS L o w -1 ra 00 rsi LCS L o w -2 o m ai C LCS L o w -3 a cra LCS M id - 1 <" X X CL LL LCS M id - 2 CL < C LL LCS M id - 3 CrLa LD rsi LCS H ig h -1 o m LCS H ig h -2 LCS H ig h -3 PFBA PFHx A PFHpA PFOA 122% 104% 89.7% 116% 100% 87.6% 117% 96.8% 86.5% 106% 89.5% 84.3% 118% 90.8% 87.8% 116% 91.1% 90.6% NA NA 87.0% NA NA 85.7% NA NA 86.0% 110% 100% 89.7% 105% 97.1% 91.4% 107% 99.6% 89.0% 98.6% 95.4% 85.5% 97.2% 94.3% 80.8% 88.8% 85.8% 75.1% NA NA 86.9% NA NA 85.2% NA NA 82.8% 95.0% 93.5% 95.2% 101% 97.1% 88.5% 83.9% 79.3% 83.9% 96.0% 92.1% 96.7% 92.1% 91.1% 82.1% 85.9% 83.5% 84.1% ISO12-01-01-04 Tennessee River Fish Study Fall 2012 PFBS Analyte Recovery (%) PFHS TotalPFOS [b] L-PFOS [b] FBSA FOSA MeFOSAA EtFOSAA 13C4-PFOA 13C4-PFOS 93.4% 91.2% 93.7% 83.5% 85.7% 88.1% 92.3% 84.4% 87.0% 94.7% 91.4% 96.3% 90.5% 89.5% 80.9% 85.4% 81.8% 83.2% 106% 104% 103% 87.0% 96.4% 95.2% 6 9 .2 % [a] 6 8 .5 % [a] 6 8 .1 % [a] 97.4% 92.0% 96.5% 89.2% 87.4% 81.1% 84.1% 80.1% 79.1% 95.2% 94.6% 94.8% 84.5% 94.3% 92.1% 105% 102% 103% 101% 91.7% 93.7% 87.5% 89.2% 78.9% 105% 102% 101% 94.8% 94.1% 93.9% 83.9% 93.5% 92.5% 93.8% 91.6% 91.6% 99.4% 90.8% 92.6% 86.0% 88.2% 78.0% 92.2% 90.7% 89.2% 105% 102% 104% 94.0% 97.2% 101% 91.2% 87.4% 85.9% 100% 99.5% 105% 94.4% 91.1% 84.9% 80.2% 79.0% 76.8% 116% 113% 108% 97.7% 110% 101% 84.2% 82.8% 85.0% 98.1% 98.1% 103% 104% 99.3% 87.2% 108% 95.8% 90.6% 109% 103% 101% 91.3% 101% 99.0% 89.6% 82.9% 84.8% 94.7% 96.1% 97.7% 93.7% 96.5% 80.4% 87.7% 82.4% 86.3% 87.2% 85.1% 88.1% 78.8% 83.3% 84.1% 84.9% 81.5% 82.4% 93.0% 87.0% 87.7% 83.2% 82.2% 76.5% 81.7% 81.2% 77.9% 85.3% 88.8% 85.7% 81.6% 93.5% 90.2% 84.4% 83.0% 81.4% 84.3% 80.9% 88.1% 86.2% 77.8% 74.9% 85.0% 77.8% 85.6% 91.1% 91.8% 88.4% 80.5% 101% 97.5% 90.3% 92.5% 86.8% 94.0% 85.3% 88.8% 88.0% 84.4% 79.1% 88.9% 85.7% 91.5% 30 Table 11. LCS Results Analytical Batch ID LCS ID LCS L o w -1 ra O m LCS L o w -2 o m CL) C LCS L o w -3 a cra LCS M id - 1 <" X X CL LL LCS M id - 2 CL < C LL LCS M id - 3 CL ra 00 rsi LCS H ig h -1 o m LCS H ig h -2 LCS H ig h -3 LCS L o w -1 ra o LCS L o w -2 rsi o m a LCS L o w -3 cra < " LCS M id - 1 X X CL LL CL LCS M id - 2 < C LL CL LCS M id - 3 ra 00 rsi LCS H ig h -1 o m bo LCS H ig h -2 LCS H ig h -3 PFBA PFHx A PFHpA PFOA 96.7% 87.9% 82.1% 104% 95.1% 89.6% 97.7% 90.0% 84.9% 98.7% 93.7% 82.0% NA NA 84.8% NA NA 74.0% NA NA 78.7% NA NA 84.5% NA NA 83.9% 101% 80.2% 88.8% 102% 92.6% 87.7% 96.2% 83.2% 82.9% 96.6% 83.8% 79.5% 120% 98.4% 103% 100% 86.7% 84.3% 110% 87.9% NA 111% 93.0% NA 105% 85.6% NA 90.3% 97.6% 92.4% 87.8% 93.1% 80.9% 83.2% 90.7% 89.7% 92.0% 91.3% 91.9% 83.4% 102% 87.3% NA NA NA PFBS 87.2% 95.2% 87.0% 85.9% 88.6% 78.9% 79.6% 87.3% 85.1% 89.6% 86.5% 81.0% 76.6% 92.1% 80.0% NA NA NA IS O 12 -01-01-04 T e n n e s s e e R iver Fish Study Fall 2 0 1 2 Analyte Recovery (%) PFHS TotalPFOS [b] L-PFOS [b] FBSA FOSA MeFOSAA EtFOSAA 13C4-PFOA 13C4-PFOS 85.2% 91.3% 88.3% 82.2% 88.3% 78.4% 80.4% 84.7% 85.7% 89.3% 85.4% 85.1% 78.5% 94.4% 82.2% NA NA NA 86.3% 91.3% 90.6% 85.8% 93.5% 80.7% 94.9% 101% 101% 97.7% 91.2% 94.1% 98.1% 116% 96.8% 130% 148% 105% 85.4% 91.3% 89.4% 86.1% 93.3% 80.1% 83.8% 89.3% 90.0% 89.6% 82.9% 85.7% 89.2% 106% 87.9% 75.6% 79.0% 67.4% 93.3% 96.6% 96.8% 87.8% 93.2% 80.4% 76.8% 81.2% 79.5% 125% 116% 106% 86.8% 100% 93.8% NA NA NA 114% 117% 97.3% 99.7% 102% 95.2% 88.4% 105% 86.4% 88.8% 84.1% 84.8% 81.3% 96.7% 83.3% 75.1% 77.7% 71.0% 93.1% 96.0% 100% 89.6% 96.9% 83.5% 84.8% 85.2% 88.4% 87.1% 85.6% 87.4% 81.2% 94.4% 81.6% 77.9% 76.9% 74.3% 84.2% 93.6% 87.7% 82.6% 87.7% 79.2% 77.8% 83.4% 80.1% 81.3% 78.2% 78.1% 75.0% 92.7% 77.6% 76.4% 76.1% 72.3% 80.4% 85.2% 85.1% 88.1% 82.8% 74.9% 76.4% 84.7% 83.7% 80.3% 75.7% 81.8% 80.5% 88.5% 77.9% 83.6% 80.6% 74.8% 86.4% 86.5% 86.3% 91.0% 88.6% 79.3% 79.6% 86.3% 88.5% 81.3% 74.2% 79.6% 81.2% 91.1% 75.6% 83.1% 86.5% 78.8% 31 Table 11. LCS Results Analytical Batch ID LCS ID LCS L o w -1 ra O rsi LCS L o w -2 o m a LCS L o w -3 cra <X" LCS M id - 1 X CL LL CL LCS M id - 2 < C LL CL LCS M id - 3 ra o rsi LCS H ig h -1 o m LCS H ig h -2 LCS H ig h -3 LCS L o w -1 ra o rsi LCS L o w -2 o m bo a LCS L o w -3 cra <X* LCS M id - 1 X CL LL CL LCS M id - 2 < C LL CL LCS M id - 3 ra o rsi LCS H ig h -1 o m bo LCS H ig h -2 LCS H ig h -3 PFBA PFHx A PFHpA PFOA 87.5% 84.1% 85.0% 88.1% 87.2% 84.7% 96.4% 93.6% 95.9% 89.4% 87.1% 89.8% 95.2% 91.4% 89.8% 95.2% 92.4% 94.4% 80.9% 93.4% 75.9% 81.4% NA 80.9% 88.1% NA NA 110% 95.7% 90.6% 120% 94.8% 93.0% 110% 93.6% 93.1% 113% 89.4% 94.6% 99.1% 78.8% 85.0% 110% 83.4% 91.5% 104% NA 87.2% 124% NA NA 112% NA NA 90.8% 95.1% 99.5% 98.6% 99.9% 100% 78.0% 76.8% 93.4% 93.0% 104% 102% 99.1% 83.8% 98.0% 87.1% 100% 90.3% PFBS 83.6% 91.2% 96.5% 86.2% 99.0% 88.5% NA NA NA 92.7% 100% 96.7% 90.9% 83.8% 93.0% NA NA NA ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Analyte Recovery (%) PFHS TotalPFOS [b] L-PFOS [b] FBSA FOSA MeFOSAA EtFOSAA 13C4-PFOA 13C4-PFOS 85.9% 85.7% 93.5% 86.1% 94.5% 94.2% 73.5% 71.3% 88.5% 96.3% 100% 95.1% 94.7% 81.6% 96.6% 82.8% 93.4% 84.4% 102% 107% 112% 99.4% 105% 105% 100% 88.7% 104% 101% 104% 98.4% 97.8% 85.9% 99.9% 107% 115% 98.3% 101% 104% 108% 95.4% 101% 100% 75.9% 70.0% 80.8% 96.8% 99.5% 92.5% 93.1% 81.9% 95.2% 77.8% 86.0% 75.6% 80.5% 84.8% 82.2% 81.9% 99.7% 94.5% 93.8% 90.6% 103% 93.7% 93.5% 94.4% 85.5% 92.5% 85.6% 89.6% 103% 96.6% 93.4% 90.4% 99.5% 94.8% 105% 94.1% 95.5% 94.2% 88.2% 81.4% 104% 93.9% 85% 109% 103% 126% 9 9 .8 0 % 118% 90.8% 95.8% 100% 82.3% 92.3% 89.5% 74.5% 75.9% 78.1% 91.0% 103% 96.7% 92.5% 79.0% 93.5% 86.0% 94.3% 81.4% 80.4% 79.9% 85.8% 81.3% 82.6% 86.8% 6 5 .3 % [a] 6 3 .2 % [a] 73.5% 90.2% 92.9% 90.9% 88.1% 74.9% 89.1% 71.0% 80.3% 79.4% 77.5% 88.7% 92.9% 90.1% 98.3% 88.8% 71.6% 70.5% 78.7% 78.6% 92.1% 90.0% 80.7% 77.5% 83.0% 81.7% 86.8% 85.7% 81.5% 94.7% 93.1% 85.3% 104% 93.7% 76.9% 76.1% 83.8% 88.6% 87.4% 87.9% 81.5% 80.0% 83.6% 86.2% 95.1% 86.4% 32 Table 11. LCS Results LCS ID PFBA PFHx A PFHpA PFOA PFBS ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Analyte Recovery (%) PFHS TotalPFOS [b] L-PFOS [b] FBSA FOSA MeFOSAA EtFOSAA 13C4-PFOA 13C4-PFOS Analytical Batch ID Low LCSAverage Low-LCS Std. Dev. Low LCSRSD Low LCS(n) 105% 8.1% 7.7% 30 95.0% 6.0% 6.3% 30 90.6% 12% 14% 30 95.0% 11% 12% 30 94.8% 6.9% 7.3% 30 93.5% 7.1% 7.6% 30 99.5% 9.4% 9.4% 30 96.2% 7.9% 8.3% 30 102% 17% 17% 30 97.0% 9.8% 10% 30 98.1% 6.6% 6.8% 30 89.9% 7.5% 8.4% 30 Mid-LCS Average Mid-LCS Std. Dev. Mid-LCS RSD Mid-LCS RSD (n) 104% 8.1% 7.8% 28 95.5% 8.5% 8.9% 28 90.9% 10% 11% 30 96.8% 8.8% 9.1% 30 92.5% 9.5% 10% 30 92.0% 7.9% 8.6% 30 98.1% 11% 11% 30 94.9% 9.5% 10% 30 95.7% 18% 19% 30 96.5% 7.6% 7.8% 30 94.5% 8.5% 9.0% 30 87.5% 8.7% 9.9% 30 High-LCS Average High-LCS Std. Dev. High LCSRSD High LCS (n) 103% 13% 13% 12 96.8% 9.1% 9.4% 7 84.9% 3.5% 4.2% 18 89.0% 8.2% 9.2% 24 84.8% 7.1% 8.3% 18 81.8% 9.5% 12% 27 106% 12% 11% 27 83.7% 11.7% 14% 30 82.3% 20% 25% 27 93.3% 13% 14% 24 86.5% 8.5% 9.8% 30 81.0% 9.5% 12% 30 Overall Study Average 104% 95.4% 89.4% 93.9% 91.6% 89.4% 101% 91.6% 93.6% 95.8% 93.0% 86.2% Standard Deviation 9.0% 7.4% 10% 10% 8.9% 9.6% 11% 11% 20% 10% 9.2% 9.3% RSD 8.6% 7.8% 12% 11% 9.7% 11% 11% 12% 21% 11% 9.9% 11% n (Total Number of LCS Results) Analytical Method Uncertainty (95%CI) 70 65 78 + 18% + 15% + 21% 84 + 20% 78 + 18% 87 + 19% 87 + 22% 90 + 23% 87 84 + 40% + 20% 90 + 18% 90 + 19% [a] R e covery w a s o u ts id e o f 100 + 30% , b u t d a ta used in s u m m a ry s ta tistics. [b ] PFOS q u a n tifie d w it h L-PFOS c a lib r a tio n , LCS r e c o v e r ie s d e t e r m in e d f o r b o th L-PFOS a n d to ta l-P F O S ( lin e a r + b r a n c h e d is o m e r s ) ; all Q Cs a n d s a m p le s q u a n tifie d f o r to ta l-P F O S N A ; N o v a lu e d e t e r m in e d f o r th e LCS e ith e r d u e t o in s tr u m e n t e r r o r o r v a lu e o u ts id e o f th e c a lib r a t io n c u rv e ra n g e f o r t h a t b a tc h 87.2% 7.4% 8.4% 30 88.0% 9.4% 11% 30 84.3% 7.8% 9.2% 30 86.5% 8.3% 9.6% 90 + 17% 89.0% 6.7% 7.5% 30 89.9% 8.8% 9.7% 30 88.3% 6.3% 7.1% 30 89.1% 7.3% 8.2% 90 + 15% 33 6.6 Laboratory Matrix Spikes (LMSs) ISO12-01-01-04 Tennessee River Fish Study Fall 2012 A s additional quality control for the study, laboratory matrix spike (LM S ) sam ples w ere prepared for ~ 1 0 % o f the study s am p les. A pproxim ately every 10th fish received in the series w a s prepared with four replicate weighings. T h e first two replicates w ere prepared as analytical sam ple and sam p le duplicate, the third replicate w as fortified with nom inal 5 ng/g (ww) o f each target analyte prior to extraction as a low -LM S and the fourth replicate fortified with nom inal 2 5 0 ng/g (ww) o f each target analyte as a high-LM S. All four replicates w ere then fortified with ISs, S R S s and carried through th e extraction and analysis procedure. In total, 21 o f th e 1 7 5 fish sam p les received in the study (1 2 % ) had a set o f L M S s prepared and analyzed. T h e L M S recovery results are s u m m arized in Table 2. T h e P F O S fortified into L M S s w a s a m ixture of linear and branched isom ers (b r-P F O S from W ellington Laboratories), and w ere quantified for total P F O S using L -P F O S calibration, as described earlier. For sam ple analytes PFBA, PFH pA, P FO A , P FB S and P F H S and most sam ples for E tFO S A A and M e F O S A A , the results for the nom inal 5 ng/g (ww) low -LM S w ere appropriate for evaluating recovery o f the target analytes based on the low level o f endogenous target analyte concentration in the equivalently non-fortified sa m p le s (i.e. appropriate fortification is considered that between 0.5-tim es to 20-tim es the endogenous level). For m any of the LM S sam ple results for P F O S and FB S A the high- LM S level w as the m ore appropriate fortification level relative to the en do g en ou s analyte level in th e non-fortified sam p le s for determ ining th e L M S recoveries. T h e overall av e ra g e L M S analyte recoveries for all target analytes w a s typically within 1 0 0 + 10% , except for FB S A which w as 8 2 .3 % . T he R S D s of L M S recoveries w ere also < 15% , excep t F B S A which had an R S D o f 2 7 % . L M S s for F B S A in runs m 1 3 0 1 2 2 b , m 1 3 0 1 2 9 b , b e 1 3 0 1 2 1 b and j1 3 0 1 3 0 a w ere outside of 100 + 3 0 % , but within 100 + 50 % and reported with exp and ed uncertainties o f + 5 0 % for those affected results in Table 1. T h e LM S recoveries w ere calculated using the following equation: LM SRecovery- (Determ ind Concentraion o f L M S - DeterminedConcentraion o f FieldSample)* ^ SpikeConcentraion 6.7 NIST SRM 1947 Results A s added Q C for each analytical batch, two accurately w eighed (nominal 0.5 gram ) aliquots of N IS T standard reference material (S R M ) 1947 w ere extracted and analyzed the sam e as sam ples. T h e results of the analysis of the N IS T S R M 1947 show ed overall averag e + standard deviation (n = 10 batches, 2 0 results) for P F O S at 5 .1 9 + 0 .5 8 ng/g (w w). This P F O S result w a s in accep tab le a g re e m e n t with the N IS T v alu e and accurate to 8 8 .0 % + 10 % with respect to the N IS T S R M 1947 reference value of 5.9 + 0 .3 9 ng/g. 6.8 Fillet Equipment Aqueous Rinseate Results A s part o f the filleting process, the eq uip m en t w a s rinsed and at tim es and th e rinseates collected for analysis. Ten aqueous equipm ent rinseate blanks w ere collected and shipped with the fish sam ples for analysis of the target analytes. Tho se sam ples w ere analyzed by 3M Environm ental Laboratory method E T S -8-044.1 and showed rinseates w ere generally less than L L O Q excep t 1.4 ng/L P F O S m e asu red in sa m p le E B L K 1 1 and P F B A m easu red at 5 .6 9 and 5 .0 9 ng/L in sam p les E B L K 0 3 and E B L K 0 0 5 . T h e results for the aq ueo u s rinseates are provided in Table 12. O verall evaluation o f rinseate blank results will be reserved for W e s to n Solutions, Inc. in their final overall study report. 34 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Table 12. C o n c e n tra tio n R e s u lts fo r F ille t-P ro c e s s E q u ip m e n t R in s e a te B lan ks SAMPLE ID LIMS ID Concentration (ng/L; parts per trillion) PFBA PFHxA PFHpA PFOA PFBS PFHS PFOS* FBSA FOSA M eFOSAA EtFOSAA EBLK01 EBLK02 EBLK03 EBLK04 EBLK005 EBLK006 EBLK07 EBLK08 EBLK09 EBLK10 EBLK11 IS O 1 2 -0 1 -0 1 -0 4 -0 1 9 IS O 1 2 -0 1 -0 1 -0 4 -0 3 6 IS O 1 2 -0 1 -0 1 -0 4 -0 5 4 IS O 1 2 -0 1 -0 1 -0 4 -0 7 1 IS O 1 2 -0 1 -0 1 -0 4 -0 8 9 IS O 1 2 -0 1 -0 1 -0 4 -1 0 7 IS O 1 2 -0 1 -0 1 -0 4 -1 2 5 IS O 1 2 -0 1 -0 1 -0 4 -1 4 3 IS O 1 2 -0 1 -0 1 -0 4 -1 6 1 IS O 1 2 -0 1 -0 1 -0 4 -1 7 9 IS O 1 2 -0 1 -0 1 -0 4 -1 8 6 < 5 .0 0 < 5 .0 0 5.69 < 5 .0 0 5.09 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 4 .8 0 < 4 .8 0 < 4 .8 0 < 4 .8 0 < 4 .8 0 < 4 .8 0 < 4 .8 0 < 4 .8 0 < 4 .8 0 < 4 .8 0 < 4 .8 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 5 .0 0 < 0 .9 2 7 < 0 .9 2 7 < 0 .9 2 7 < 0 .9 2 7 < 0 .9 2 7 < 0 .9 2 7 < 0 .9 2 7 < 0 .9 2 7 < 0 .9 2 7 < 0 .9 2 7 1.40 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 2 .0 0 < 9 .9 8 < 9 .9 8 < 9 .9 8 < 9 .9 8 < 9 .9 8 < 9 .9 8 < 9 .9 8 < 9 .9 8 < 9 .9 8 < 9 .9 8 < 9 .9 8 < 1 5 .0 < 1 5 .0 < 1 5 .0 < 1 5 .0 < 1 5 .0 < 1 5 .0 < 1 5 .0 < 1 5 .0 < 1 5 .0 < 1 5 .0 < 1 5 .0 * R e s u lts o f LCSs p r e p a r e d in w a t e r d u r in g t h e a n a ly s is in d ic a te d an a v e ra g e + RSD a c c u ra c y f o r PFOS o f 9 6 .9 % + 3 .5 % a n d w i t h an a n a ly tic a l m e th o d u n c e r t a in ty (9 5 % CI) o f + 1 7 % ; a v e ra g e + RSD a c c u ra c y fo r PFBA w a s 100 % + 0 .5 8 % w ith an a n a ly tic a l m e th o d u n c e rta in ty (95% CI) o f + 16% . 35 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 7 Conclusion T h e determ in ed concentrations o f perfluorinated chem icals (P F C s ) m easu red in fish collected from th e T e n n e s s e e R iver in th e Fall o f 2 0 1 2 w e re reported. T h e s e analyses w e re conducted as 3 M E nvironm ental analytical project IS O 1 2 -0 1 -0 1 -0 4 and w e re perform ed in support o f the W e s to n Solutions, Inc. study plan, titled "R evised W o rk Plant for Fall 2 0 1 2 Fish and S urface W a te r Sam pling in the T e n n e s s e e R iver", and d ated O cto b er 2 0 1 2 . During the Fall of 2 0 1 2 , W eston Solutions, Inc. personnel caught five largem outh bass and five catfish from each of fifteen pre-defined fish sam pling reaches located within the confines of the W ilson and W h e e le r R eservoirs o f th e T e n n e s s e e River, located n e a r D ecatur, A L. T h e fish w e re filleted (skin rem oved ) in th e field. A total o f 1 5 0 left side fillets and 15 random ly selected right side fillets (as field duplicates) w e re rem oved and then shipped to T es tA m e rica (Burlington, V T ) for hom ogenization o f the fillets. Fro zen fillet h om o g en ates w e re then shipped on dry ice to the 3M Environm ental Laboratory for extraction and quantitative analysis of PFC s. An additional six process controls for evaluating the filleting process, three process controls for evaluating the hom ogenization process, and one N IS T fish tissue standard reference material (S R M 1 947) w e re also received. A total o f 175 fish tissue sam ples w ere received and quantitatively analyzed. T h e P FC s analyzed for w ere perfluorobutanoate (PFBA ), perfluorohexanoate (PFH xA ), perfluoroheptanoate (PFH pA ), perfluorooctanoate (PFO A ), perfluorobutane sulfonate (P FB S ), perfluorohexanesulfonate (P F H S ), perfluorooctanesulfonate (P F O S ) , perfluorobutanesulfonamide (FB SA ), perfluorooctanesulfonamide (F O S A ),2-[N m ethyl-FO SA]acetic acid (M eF O S A A ) and 2-[N-ethyl-FO SA ]acetic acid (E tFO S A A ). E ach s am p le w a s extracted in duplicate for determ ination o f th e a v e ra g e P F C concentrations, and approxim ately every10th fish s a m p le (21 in total; 12 % o f total fish) had tw o laboratory matrix spike (L M S ) sam p les prepared from a third and fourth 0.5 gram aliquot and fortified with known quantities o f target analytes. T h e L M S recovery results are show n in Table 2 and av e ra g e +standard deviation recovery results w e re within 1 0 0 + 1 0 % for all analytes, with R S D s less than 15% , except F B S A which had a recovery at 8 2 .3 % + 2 2 % (R S D = 2 7 % ). Based on the L M S recovery results, one P FB S result for sam ple M S F 011 (IS O 1 2 -0 1 -0 1 -0 4 -0 1 1 ) and P F O A and P F B S results for fish M S F 0 4 3 (IS O 1 2 -0 1 -0 1 -0 4 -0 4 3 ) w ere not reported (N R ) due to LM S recoveries outside of 100 + 50% . Laboratory control spikes (L C S s ) w e re prepared in control bluegill fillet tissue at 3 levels, each level in triplicate, and analyzed with each analytical batch. A v e ra g e L C S recoveries w e re within 1 0 0 + 1 4 % at all L C S levels, and overall, for each analyte. T h e L C S statistical results (for all LC S levels) w ere used to determ ine the analytical method uncertainty (95% C I) for the dataset, and w ere + 18% for PFBA, + 15% for PFH xA , + 21 % for PFH pA, + 20% for P FO A , + 18% for P FB S , + 19% for P F H S , + 22 % for total-P FO S (linear + branched isom ers), + 4 0 % for FBSA, + 20% for FO S A , + 18% for M eF O S A A and + 19% for EtFO SAA. T h e N IS T standard referen ce m aterial S R M 1 9 4 7 (L a k e M ichigan fish tissue) w a s analyzed in duplicate as part of each analytical batch. T he overall average P F O S value + standard deviation w as determ ined at 5 .1 9 + 0 .5 8 ng/g (ww) over ten analytical batches (n = 2 0 results). This result w a s accurate to 8 8 .0 % and in accep tab le a g re e m e n t with the S R M 1 9 4 7 P F O S reference value of 5.9 + 0.39 ng/g (ww). T w o stable-isotope labeled surrogate recovery standards (S R S s ) C 4-P F O A and C 4-P F O S w e re ad ded to each s a m p le aliquot prior to extraction. A v e ra g e S R S recoveries for all th e fish tissue sam p les analyzed (n = 17 5 ) w e re 8 5 .3 % for 13C 4-P F O A and 8 8 .1 % for 13C 4-P F O S . T h e relative standard deviation (R S D ) for the average S R S recoveries showed excellent method precision with R S D s o f 5 .2 % for 13C 4-P F O A and 3 .8 % for 13C 4-P F O S . O verall, th e s a m p le results in Table 1 show ed that P F O S and F B S A w e re detected with highest frequency in T e n n e s s e e R iver fish s am p les and with the highest concentrations m easured. P F O S concentrations ranged from <0 .3 6 6 ng/g (ww) to 5 99 ng/g (ww). FB S A 36 IS 0 12-01 -01 -04 Tennessee River Fish Study Fall 2012 ranged from <0.250 ng/g (ww) to 567 ng/g (ww). Similarly, FOSA, MeFOSAA and EtFOSAA were detected at relatively high frequencies, but generally were much lower in concentration than PFOS and FBSA. The remaining analytes PFBA, PFHxA, PFHpA, PFOA, PFBS and PFHS were detected much less frequently and at relatively low concentrations. 8 Data/Sample Retention All fish samples and associated project data (hardcopy and electronic) for LIMS project IS01201-01-04 will be archived according to 3M Environmental Laboratory standard operating procedures 9 List of Attachments Attachment A: Preparation Forms, Raw Data and Chromatograms (A va ila b le U po n R e q u e st) 10 Signatures Report Approval: Cleston C. Lange, Ph.D., Principal Analytical Investigator s/c: h Date William K. Reagen, Ph.D., 3M Environmental Laboratory Management Date 37 ISO12-01-01-04 Tennessee River Fish Study Fall 2012 Attachment A PFC Results from Analysis of Bass & Catfish Control Materials Used for Fillet-Process Controls Table A1. C o n c e n tra tio n R e s u lts fo r B ass & C atfish Fillet C on trol M a te ria ls (R ig h t S id e Fillets) SAMPLE ID Average Concentration (ng/g; w et w t.) PFBA PFHxA PFHpA PFOA PFBS PFHS PFOS* FBSA FOSA MeFOSAA EtFOSAA T N 1 2 -0 3 6 4 -1 /1 3 -L M B a ss-R ig h t S ide F ille t TN 1 2 -0 3 6 4 -1 /1 3 -L M B a ss-R ig h t S ide F ille t TN 1 2 -0 3 6 4 -1 /1 3 -L M B a ss-R ig h t S ide F ille t TN 1 2 -0 3 6 5 -1 /1 3 -C a tfis h -R ig h t S ide F ille t TN 1 2 -0 3 6 5 -1 /1 3 -C a tfis h -R ig h t S ide F ille t TN 1 2 -0 3 6 5 -1 /1 3 -C a tfis h -R ig h t S ide F ille t < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 <1.00 <1.00 <1.00 <1.00 <1.00 <1.00 < 0 .0 5 0 0 < 0 .0 5 0 0 < 0 .0 5 0 0 < 0 .0 5 0 0 < 0 .0 5 0 0 < 0 .0 5 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 4 .2 7 4.12 3.85 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .1 0 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .2 5 0 < 0 .3 5 0 < 0 .3 5 0 < 0 .3 5 0 < 0 .3 5 0 < 0 .3 5 0 < 0 .3 5 0 [a] LMSs fo rtifie d w ith n o m in a l 1 0 n g /g o f each ta rg e t ana lyte s w e re p re p a re d w ith th e sam ple s and re co ve rie s w e re all w ith in 100 + 20% LLOQs s h o w n are n o m in a l fo r a n o m in a l 0 .5 g tissu e s a m p le e x tra c te d and ana lyzed R esults are s h o w n fo r rig h t sid e fille ts o n ly ; re m o v e d a t th e la b o ra to ry and k e p t a t th e la b o ra to ry fo r analysis. In ta c t le ft sid e fille ts a tta c h e d to fis h b o d ie s s e n t to th e fie ld fo r fille t-p ro c e s s c o n tro ls . 38 Attachment B Preparation Forms, Raw Data and Chromatograms ISO12-01-01-04 Tennessee River Fish Study Fall 2012 (Available Upon Request) 39 APPENDIX D AXYS LABORATORY ANALYTICAL DATA PACKAGE www.axysanalytical.com >)][( A X Y S A xys A n a ly tic a l S e rvices Ltd 2045 Mills Road West SIDNEY, BRITISH COLUMBIA, CANADA V8L 5X2 TEL 250-655-5800 FAX 250-655-5811 w w w .a x y s a n a ly tic a l.c o m AXYS Client No.: Client Address: 4731 Weston Solutions Inc. 1400 Weston Way West Chester, PA, US, 19380 The AXYS contact for these data is Candice Navaroli. Page 1 of 307 www.axysanalytical.com PERFLUORINATED ORGANIC ANALYSIS AQUEOUS SAMPLES PROJECT NAME: Fall 2012 Tennessee River Sampling Contract: 4731 Data Package Identification: DPWG42492 Analysis WG42273 & WG42428 17 January 2013 Page 2 of 307 www.axysanalytical.com Weston Solutions Inc. AQUEOUS SAMPLES PROJECT NAME: Fall 2012 Tennessee River Sampling NARRATIVE PERFLUORINATED ORGANIC ANALYSIS AXYS METHOD: MLA-06Q 4731: L18 9 8 9 -1 t o -1 4 17 January 2013 This narrative describes the analysis of fourteen aqueous sam ples for the determ ination of perfluorinated organic co m p ou n d s using high p erfo rm an ce liquid ch ro m ato g rap hy/tan d em m ass sp ectrom etry (H P L C /M S -M S ), SAMPLE RECEIPT AND STORAGE T h e sa m p le s w e re received on the 14th of D e c e m b e r 2 0 1 2 . U p o n receipt, th e te m p e ra tu re for th e s a m p le s ranged from 2.1 to 4 .7 C m arginally exceeding the required sam ple storage conditions of 0 to 4 C for so m e sam ples. This variance from m ethod criteria w as d e e m e d to not be of sufficient m agnitude to affect data quality. Details o f sam ple conditions on receipt are provided on the sam p le receiving records included with this data package. T h e sam ples w ere stored at 4 C prior to extraction and analysis. SAMPLE PREPARATION AND ANALYSIS T h e extraction and analysis procedures w ere followed as outlined with A X Y S M ethod M L A -060: A n a ly tic a l P ro c e d u re fo r th e A n a ly s is o f P e rflu o rin a te d O rg a n ic C o m p o u n d s in A q u e o u s S a m p le s b y L C -M S /M S . A m etho d s u m m a ry ( M S U -0 6 0 ) of th e m eth o d is in clud ed w ith this d a ta p a c k a g e . T h e client and associated Q C sam ples (consisting of a laboratory procedural blank, a lab-generated reference sam ple referred to as an Ongoing Precision and R ecovery (O P R ) sam p le and a duplicate sam ple) w ere analysed collectively under tw o batches as W G 4 2 2 7 3 and W G 4 2 4 2 8 . T h e com position of th ese analysis batches are shown on th e correlation table, an d also on th e batch list heading o f the extraction w o rku p sh e e ts provided with the d a ta package. For each sam ple, an accurately w eighed subsam ple of approxim ately 0 .5 L w a s spiked with labeled quantification standards (surrogates), extracted and then cleaned up using S P E cartridges. T h e cleaned extract fo r e a c h s a m p le w a s sp iked w ith lab e le d re c o v e ry (in tern al) stan d ard s, and instrum entally a n alysed using liquid chrom atography/m ass spectrom etry (L C -M S /M S ). CALCULATIONS T a rg e t analyte concentrations w e re determ ined by isotope dilution quantification procedures, com paring the a re a of the quantification o f the target analyte to that of the labeled quantification standard (surrogate) and correcting for response factors. Linear regression equations w e re determ ined from a m ulti-point calibration series with 1/X w eighting fit. L a b e le d a n a ly te reco very w a s d ete rm in e d by using a v e ra g e re s p o n s e factors d eterm in ed from the initial m ultipoint calibration an d w e r e u sed as g e n e ra l d a ta quality indicators. R e c o v e ry valu es of th e reco very (injection) standard w e re estim ated by external standard quantification and used as indicators o f the extent of signal suppression or enhancem ent. S a m p le specific detection limits (S D L ) w e re d eterm in ed from the analysis d ata by converting th e area equivalent to 3 x th e c h ro m ato g rap h ic n oise to a concen tratio n in th e s a m e m a n n e r th at p e a k a re a s a re converted to concentrations. F o rm u la e used for th e conversion of th e raw c h ro m a to g ra m d a ta to concentration a re provided in the m ethod sum m ary document, M S U -060. REPORTING CONVENTIONS For internal tracking, A X Y S assig n ed W e s to n Solutions inc. contract n um ber 4 7 3 1 . A X Y S logged the sam ples u n d e r u n iq u e lab o rato ry id en tifiers L 1 8 9 8 9 -X ; w h e re X is a n u m e ra l. All d a ta re p o rts p ro vid ed re fe re n c e both th e A x y s ID a n d th e clien t s a m p le identifier. T o a ssist in locating d ata, a tab le correlating A xys ID s with the client Page 3 of 307 www.axysanalytical.com s a m p le n u m b ers is included w ith this d a ta p a c k a g e . T h e rep o rt fo rm s w e re g e n e ra te d using Labo ratory Inform ation M anagem ent Software (LIM S). A n y additional w o rk p erfo rm ed a fte r th e initial instrum ental analysis of th e s a m p le e x tra c t is indicated by a suffix added to the A X Y S ID . T h e notation used to identify sam ples reported from extra w ork d ata or that w e re used as the duplicate o f a client sam ple for this d ata package includes: R = rep eat analysis using a fresh aliquot of sam ple (A) = the parent sam ple for a duplicate pair T h e following d a ta qualifier flags w e r e u sed in this d a ta p ackag e: B = a n a ly te found in s a m p le a n d a s s o c ia te d b lan k U = identifies a com pound that w a s not detected T h e reporting limit (R L ) w as defined as th e concentration equivalent to the low est calibration standard analyzed in th e initial calib ratio n (C S O ), c o rre c te d fo r s a m p le s ize , o r th e s a m p le s p e c ific d e te c tio n limits, w h ic h e v e r w a s g re a te r. S a m p le a n a ly te co ncen tratio n s a re reported in units o f n a n o g ra m s p e r litre (n g /L ). R esults a re reported to three significant figures. T h e analysis results for each sam ple are provided on Analysis R eport form s 1A and 2. QA/QC NOTES T h e laboratory procedural blank, O P R , and duplicate sam ples w ere prepared alongside the client sam ples, and w ere subjected to the s am e analytical procedures. T h e results w ere evaluated through com parison to the batch Q C sam ples. S am p le analyte concentrations are not blank corrected and should be evaluated with consideration of the laboratory procedural blank results. By virtue of the isotope dilution standard quantification procedures, d ata are recovery corrected for possible losses during extraction and clean-up. 9 T h e S ig n a l/N o is e (S /N ) ratios w e r e m e a s u re d a s `0 ' fo r s o m e co m p o u n d s in th e Q C s a m p le s an d sa m p le data. This has been determ ined to be a limitation o f the softw are and does not affect the data 9 All linearity, calibration verification, O P R and labelled com pound recovery specifications w e re m e t with the following exception: W G42273 T h e ta rg e t a n a ly te P F H p A w a s d e te c te d in th e laboratory procedural b lan k (A X Y S ID: W G 4 2 2 7 3 - 1 0 1 ) a t a concentration (1 .4 8 ng/L) ab ove th e reporting limit (R L ) o f 1.0 0 ng/L. Subsequently, th e results reported for this com pound for th e sa m p le and duplicate sa m p le (A X Y S IDs: L 18989-11 and W G 4 2 2 7 3 -1 0 3 ) h ave been flagged with a `B ' on th e report fo rm s. T h e detection o f this c o m p o u n d in th e laboratory pro cedu ral b lan k s a m p le should be considered w hen reviewing the results for these two sam ples. . W G42428 No issues to report ANALYTICAL DISCUSSION W G42273 A s indicated ab o v e , th e ta rg e t an a ly te P F H p A w a s d ete c te d in the laboratory b lan k s a m p le (A X Y S ID: W G 4 2 2 7 3 10 1 ) above th e reporting limit. F or m ost of the client sam ples, th e s a m e com pound w a s detected a t sim ilar concentrations. Accordingly, w h ere possible, the back-up portion of the client sam p les w a s used to rep eat the analysis with the results reported from th e second (repeat) analysis batch (W G 4 2 4 2 8 ), as indicated by that addition of the suffix `R ' to the A X Y S ID. For the D A L -S W -T 6 0 6 -D U P A -1 2 1 2 0 4 and duplicate s am p les (A X Y S IDs: L 1 8 9 8 9 - Page 4 of 307 www.axysanalytical.com 11 and W G 4 2 2 7 3 -1 0 3 ), no b ack-u p portion w a s a vailab le to re p e a t th e analysis. S u b seq u ently, the results for th e s e sam p les w e re reported from the initial an alysis batch (W G 4 2 2 7 3 ). W G42428 N o analytical difficulties w e re encountered for this analysis. DATA PACKAGE T h is d a ta p a c k a g e is assig n ed a unique identifier, D P W G 4 2 4 9 2 , sh ow n on th e front p ag e. T h e following d o c u m e n ts a re includ ed in th e d a ta p a c k a g e : Method sum m ary S am ple Correlation Table S am ple Receiving Docum entation Laboratory extraction W orksheets S am p le d ata reports (in o rder of Axys S a m p le ID ) Laboratory Q C data reports Instrum ental Q C data reports Laboratory Q C and sam ple raw data Instrum ental Q C raw data Accreditation Scope 1 certify that this data package is in compliance with the terms and conditions of the contract, both technically and for completeness, except for the conditions detailed above. In addition, I certify that to the best of my knowledge and belief the data as reported are true and accurate. The following signature authorizes on behalf of Axys Analytical Services Ltd the release of the data contained in this data package. Signed: A ndrew Porat, D ata Validation C hem ist D ate Signed m Page 5 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. Method Summary Analytical Procedure for the Analysis of Perfluorinated Organic C om pounds in A queous Sam ples by LC -M S/M S by A XYS Method MLA-060 Rev. 10 Ver. 03 Method MLA-060 describes the analysis of perfluorinated organic compounds (PFC) in aqueous samples. Typical quantification limits are in the range of 1 - 2 ng/L for a 0.5 L sample size. ANALYTES OF INTEREST Perfluorobutanoate (PFBA) Perfluoropentanoate (PFPeA) Perfluorohexanoate (PFHxA) Perfluoroheptanoate (PFHpA) Perfluorooctanoate (PFOA) Perfluorononanoate (PFNA) Perfluorodecanoate (PFDA) Perfluoroundecanoate (PFUnA) Perfluorododecanoate (PFDoA) Perfluorobutanesulfonate (PFBS) Perfluorohexanesulfonate (PFHxS) Perfluorooctanesulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) 1 EXTRACTION AND CLEANUP Sample size may be up to 1000 mL. Samples are stored in HDPE (high density polyethylene) containers. Samples are filtered, adjusted to pH 6.5, spiked with surrogate standards and extracted by solid phase extraction (SPE) using weak anion exchange cartridges. Wash and elution procedures are chosen to meet various analysis requirements. The eluates are spiked with recovery standards and analyzed by LC-MS/MS. Calibration solutions are processed through SPE in the same way as the samples. QUALITY ASSURANCE / QUALITY CONTROL All samples are analyzed in batches. The composition of a batch is detailed on a batch sheet. Each batch has the following composition: Batch Size - Each batch consists of test samples and additional QC samples. Blanks - Blanks are analyzed with a minimum frequency of 5% of client samples. Duplicates - With each analysis batch containing greater than six (6) test samples, or as required by contract, a duplicate sample is analyzed, provided there is sufficient sample. Matrix Spike/Matrix Spike Duplicate analyzed upon client request. OPR (Spiked Reference Sample) - OPRs are analyzed with a minimum frequency of 5% of client samples. MSU-060 Rev 06, 06-Sep-2012 Summary of AXYS Method MLA-060 Rev. 10 Ver. 03 Page 1 of 6 Page 6 of 307 www.axysanalytical.com QC Specification: Procedural Blank Levels and OPR Recoveries Analyte P e rflu o ro b u ta n o a te P e rflu o ro p e n ta n o a te P e rflu o ro h e x a n o a te P e rflu o ro h e p ta n o a te P e rflu o ro o c ta n o a te P e rflu o ro n o n a n o a te P e rflu o ro d e c a n o a te P e rflu o ro u n d e c a n o a te P e rflu o ro d o d e c a n o a te (P F B A ) (P F P e A ) (P F H x A ) (P F H p A ) (PFO A) (PFNA ) (PFDA ) (P F U n A ) (P F D o A ) Procedural Blank Level ng/sample 2 < 0 .2 5 < 0 .2 5 < 0 .2 5 < 0 .2 5 < 0 .2 5 < 0 .2 5 < 0 .2 5 < 0 .2 5 < 0 .2 5 OPR Recovery Range (% )1 80 - 1 2 0 1 80 - 1 2 0 1 80 - 1 2 0 1 80 - 1 2 0 1 80 - 1 2 0 1 80 - 1 2 0 1 80 - 1 2 0 1 80 - 1 2 0 1 80 - 1 2 0 1 P e rflu o ro b u ta n e s u lfo n a te P e rflu o ro h e x a n e s u lfo n a te P e rflu o ro o c ta n e s u lfo n a te (P F B S ) (P F H x S ) (PFO S) < 0 .2 5 < 0 .2 5 < 0 .2 5 70 - 130 70 - 130 70 - 130 Perfluorooctane sulfonam ide (PFO SA) < 0 .2 5 70 - 130 1 Marginal exceedance allowance - recovery for 2 compounds may be 75-125% and for one compound 70-130%. 2 Reporting limits (based on the lowest calibration standard and routine final extract volume of 4 mL) may exceed the stated blank criteria. QC Specification: Surrogate Standard Recoveries (Calibration Solutions and Samples) Surrogate Standard 13C4-Heptafluorobutyric acid (13C4-PFBA) 13C2-Perfluorocaproic acid (13C2-PFHxA) 13C2- Perfluorooctanoic acid (13C2-PFOA) 13C5-Heptadecafluorononanoic acid (13C5-PFNA) 13C2- Perfluorodecanoic acid (13C2-PFDA) 13C2-Perfluoro-n-(1,2)dodecanoic acid (13C2-PFDoA) 18O2-Perfluorohexanesulfonate (18O2-PFHxS) 13C4-Perfluorooctanesulfonate (13C4-PFOS) 13C8-Perfluoro-1-octanesulfonamide (13C8-PFOSA) 1 Lower recoveries may be accepted based on application and professional judgment 2 The recovery range for 13C8-PFOSA is a guideline Recovery Range 1 20- 150% 40 - 150% 40- 150% 40- 150% 40- 150% 40 - 150% 40 - 150% 40- 150% 40 - 150% 2 Page 7 of 307 www.axysanalytical.com QC Specification Table: Other Parameters QC Parameter Specification In s tru m e n t S e n s itiv ity D a ily , S :N > 3:1 fo r a ll a n a ly te s fo r lo w e s t c a lib ra tio n s ta n d a rd . In itia l C a lib ra tio n (n a tiv e com pounds) R u n in itia lly , a n d a s re q u ire d to m a in ta in c a lib ra tio n v e rific a tio n a n d in s tru m e n t s e n s itiv ity . (1 /x ) w e ig h te d q u a d ra tic , e x c lu d e o rig in . C a lc u la te d c o n c . 7 5 -1 2 5 % o f a c tu a l (lo w e s t c a l m a y b e 7 0 -1 3 0 % ), R 2 > 0 .9 9 0 . S u r r o g a te r e c o v e r ie s m u s t fa ll w ith in th e s a m e lim its a s f o r th e s a m p le s in th e ta b le a b o v e . C o n tin u in g C a lib ra tio n V e rific a tio n (n a tiv e com pounds) R u n e v e ry 2 0 s a m p le s o r m o re fre q u e n tly , q u a n tify a g a in s t I-C A L . C a lc u la te d c o n c . 7 0 -1 3 0 % a c tu a l fo r a m a x im u m o f th re e c o m p o u n d s w ith th e re m a in d e r 8 0 -1 2 0 % o f a c tu a l. S u r r o g a te r e c o v e r ie s m u s t fa ll w ith in th e s a m e lim its a s f o r th e s a m p le s in th e ta b le a b o v e . In s tru m e n ta l C a rry o v e r an d In s tru m e n t B a c k g ro u n d E v e ry In itia l C a lib ra tio n , C a l/V e r, o r S P M : < 0 .3 % c a rry o v e r a n d a re a re s p o n s e o f a n a ly te s in in s tr u m e n t b la n k < 8 0 0 . D u p lic a te S a m p le s o r M S /M S D If c o n c . > 5 tim e s R .L ., R P D < 4 0 % If c o n c . < 5 tim e s R .L ., d iffe re n c e b e tw e e n p a irs < R .L . m Page 8 of 307 www.axysanalytical.com ANALYSIS BY LC-MS/MS Analysis of sample extracts for perfluorinated organics by HPLC-MS/MS is performed on a high performance liquid chromatograph coupled to a triple quadrupole mass spectrometer. The mass spectrometer is run at unit mass resolution in the Multiple Reaction Monitoring (MRM) mode. Instrument specifications: Instrument LC Column Ionization Acquisition Injection Volume Waters 2690 or Waters 2795 HPLC, Micromass Quattro Ultima MS/MS Waters Xtera C18MS Reverse Phase C18, 10.0 cm, 2.1 mm i.d., 3.5 pm particle size (or equivalent) Negative Ion Electrospray MRM mode, unit resolution 15 pL LC-MS/MS Operating Conditions: Time (min) 0 .0 1 .0 5 .0 8 .5 LC Gradient Program Flow m ixture1 1 5 % e lu e n t A 8 5 % e lu e n t B 1 5 % e lu e n t A 8 5 % e lu e n t B 7 0 % e lu e n t A 3 0 % e lu e n t B LC Flow Rate Program 0 .1 5 m L /m in 0 .1 5 m L /m in 0 .2 0 m L /m in 1 0 0 % e lu e n t A 0 .2 0 m L /m in General LC Conditions G ra d ie n t Curve C o lu m n T e m p (C ) 40 1 M a x P re s s u re (bar) 300 1 MS Conditions 4 S o u rc e T e m p (C ) 4 D e s o lv a tio n T e m p (C ) 120 300 11 1 0 0 % e lu e n t A 1 1 .3 - 1 4 .5 1 5 % e lu e n t A 8 5 % e lu e n t B 0 .2 0 m L /m in 0 .2 0 m L /m in 4 C a p illa ry V o lta g e 2 G ases 2 .7 5 ~ 7 0 L /h r con e ~ 3 0 0 L /h r d e s o lv a tio n 1 E lu e n t A = 9 0 % C H 3C N ( a q u e o u s ) E lu e n t B = 1 3 m M a m m o n iu m a c e ta te in 0 .1 % a c e tic a c id (a q u e o u s ) Initial calibration of the LC-MS/MS instrument is performed by the analysis of six or more calib ration solutions. A mid-level calibration standard is analyzed to verify the initial calibration after every 20th sample (including QC samples) injected at a minimum. All calibration solutions go through the same SPE extraction/cleanup procedures as the samples. Page 9 of 307 www.axysanalytical.com ANALYTE IDENTIFICATION Positive identification of target PFC, surrogate standard and recovery standards require: > 3:1 S:N for parent ion to daughter ion transition. Compound retention time must fall within 0.4 minutes of the predicted retention times from the daily Calibration Verification. Natives with labelled surrogate standards must elute within 0.1 minutes of the associated labelled surrogates. QUANTIFICATION AND DATA REPORTING PROCEDURES Target compounds are quantified using the internal standard method, comparing the area of the quantification ion to that of the 13C-labelled standard and correcting for response factors. Quadratic calibration equations are determined from a multi-point calibration series with 1/X weighing fit as described by the following general equation: Y= a + bX + cX2 (general quadratic equation) where Y = (area target/area surr) x weight surr X = weight target a,b,c are empirical constants Concentrations in samples are determined as: - b Sample Conc = -------- ( ( area o f t arg et \\ b2 - 4c a - x weight sur area o f sur 2c x sample size The recovery of the surrogate standard is calculated (by internal standard quantification against the recovery standard using an av erage RRF) and monitored as an indication of overall data quality. Final target concentrations are recovery corrected by this method of quantification. Sample Specific Detection Limits (SDL) are determined .by converting the area equivalent of 3.0 times the estimated chromatographic noise height to a concentration in the same manner that target peak responses are converted to final concentrations. The SDL accounts for any effect of matrix on the detection system and for recovery achieved through the analytical work-up. Results are reported to the greater of the SDL or the concentration equivalent to the lowest calibration standard analyzed. MSU-060 Rev 06, 06-Sep-2012 Summary of AXYS Method MLA-060 Rev. 10 Ver. 03 Page 5 of 6 Page 10 of 307 www.axysanalytical.com Analytes, Ions and Quantification References Target Analyte Typical Retention Time (minutes) Target Analytes Perfluorobutanoate (PFBA) Perfluoropentanoate (PFPeA) Perfluorohexanoate (PFHxA) Perfluoroheptanoate (PFHpA) 5.0 5.8 6.2 6.6 Perfluorooctanoate (PFOA) 7.0 Perfluorononanoate (PFNA) Perfluorodecanoate (PFDA) Perfluoroundecanoate (PFUnA) Perfluorododecanoate (PFDoA) 7.4 7.9 8.5 9.0 Perfluorobutanesulfonate (PFBS) 6.3 Perfluorohexanesulfonate (PFHxS) 7.2 Perfluorooctane sulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) 2 Surrogate Standard 13C4-Heptafluorobutyric acid (13C4-PFBA) 13C2-Perfluorocaproic acid (13C2-PFHxA) 13C2-Perfluorooctanoic acid (13C2-PFOA) 13C5-Heptadecafluorononanoic acid (13Ca-PFNA) 13C2-Perfluorodecanoic acid (13C2-PFDA) 13C2-Perfluorododecanoic acid (13C2-PFDoA) 18O2-Perfluorohexanesulfonate (18O2-PFHxS) 8.2 9.9 5.0 6.2 7.0 7.4 7.9 9.0 7.2 13C4-Perfluorooctanesulfonate (13C4-PFOS) 13C8-Perfluorooctane sulfonamide (13C8-PFOSA) Recovery Standard 13C2-2H-Perfluoro-2-decenoic acid (13C2PFOUEA) 13C4-Perfluorooctanoic acid (13C4-PFOA) 8.2 9.9 7.3 6.9 Parent Daughter Ion Mass Ion Mass Q u a n tifie d Against < GO LL CL o1 CO 213 169 263 219 13C2 -PFHxA 313 269 13C2-PFHxA 363 319 13C2-PFHxA 413 369 (169)1 13C2-PFOA 463 419 13C5-PFNA <Q <Q LL LL CL CL 11 OCM OCM CO CO 513 469 563 519 613 569 13C2 -PFDoA 299 80 (99)1 18O 2 -P F H x S 399 80 (99/119)1 18O 2 -P F H x S cn cn OO LL LL CL CL o1 o1 CO CO 499 80 (99)1 498 78 217 172 13C2-PFOUEA 315 270 13C2-PFOUEA 415 370 13C4-PFOA 468 423 13C2-PFOUEA 515 470 13C2-PFOUEA 615 570 13C2-PFOUEA 403 84 (103)1 13C2-PFOUEA 503 80 (99)1 13C2-PFOUEA 506 78 13C2-PFOUEA 459 394 417 372 - 1 Alternate transition within brackets, may be used if necessary to avoid interference. 2 13C8-PFOSA may be used as an alternate surrogate for PFOSA if deemed necessary to improve data quality. Page 11 of 307 www.axysanalytical.com Weston Solutions Inc. CORRELATION TABLE PERFLUORINATED ORGANIC ANALYSIS Lab Name: AXYS Analytical Services Ltd. Project Name:Fall 2012 Tennessee River Sampling Data Package Identification: DPWG42492 Project Manager: Candice Navaroli Contract No: 4731 AXYS Method: MLA-060 Program: Aqueous Samples Client Sample No. Lab Sample ID LAB BLANK OPR WG42273-101 WG42273-102 DAL-SW-T606-DUPA-121204 L18989-11 WG42428-103 DUPLICATE LAB BLANK OPR WG42428-101 WG42428-102 DAL-SW-R02L3-DUPA-121116 DAL-SW-R15L1-DUPA-121117 DAL-SW-R10L3-DUPA-121119 DAL-SW-R05L1-1-121119 DAL-SW-RO6L2-DUPA-121120 DAL-SW-T101-DUPA-121203 DAL-SW-T202-DUPA-121203 DAL-SW-T303-DUPA-121203 DAL-SW-T404-DUPA-121203 DAL-SW-T505-DUPA-121204 DAL-SW-T707-DUPA-121204 DAL-SW-T808-DUPA-121205 DAL-SW-T909-DUPA-121205 L18989-1 L18989-2 L18989-3 L18989-4 L18989-5 L18989-6 L18989-7 L18989-8 L18989-9 L18989-10 L18989-12 L18989-13 L18989-14 Page 12 of 307 www.axysanalytical.com COaxys Axys Analytical Services Ltd 2045 Mills Road West TEL: (250) 655-5800 Sidney, British Columbia, Canada V8L 5X2 FAX: (250) 655- 5811 R E P O R T TO: Company Address 4D A A P iP .U f ) - T A ^ IN V O IC E TO : ^ Compar y Addres s CHAIN OF CUSTODY i, r 1. AXYS CLIENT #: 4 T S ANALYSIS REQUESTED U /K k , ? it/tlt* . H O /) A J e Z h n \a ) m / J & \ fZ o a d > .H iJ e S f V p fA ^ v r fa , 3S 7O S Contact ju /Z j V o J rZ Phone 5 9 5 5 5 FAX E-mail a i> ^ Project Name/Number: % 4o < )$ s i Conta ct Phon e FA x E-msil A k to 6 ? /o Sampler's Name: V ova* ^0/ 7d / z> 4 0 / '' r l,|.L . / & * *foJ*-*4 v r* VJ U. Client Sample Identification Matrix Sampling Date S ig n a tu re :^ ^ ^ Sampling Container Time Type/No. ZJ Preservative Y /N AXYS Lab ID Lab use only Au-S v j- R U - W it^ D ^ t - v i - & j & t J , M P`V t L fU ^ i 'lL -S W - e i'i.l-'b s P A * VJ w vo W vo '111t f i o n . <3 A il|iT | | - iW T r S " t) l/l ^ / . V ilio o fb '.A T * a ja s /iS s f "*> I i~ N/ 'W 1 Y "T V ~3 -S Y N ____________ J I Z . ________________ Relinq qature) Date iquished by (Signature) Remarks / Type Of Preservative Date Time tyop Time Received by (Signature) .......... . Date I H ' h & v Received by (Signature) Time ! 0 !. s s Date_______________________T_irmrre__________ le& se A re& lh is courier e X Waybill No. 3 & Temp C Custody Seal # Seal Intact Sample Tags Sample Receipt Cooler Y/ N Y /N m Page 13 of 307 www.axysanalytical.com A axys Axys Analytical Services Ltd 2045 Mills Road West TEL: (250) 655-5800 Sidney, British Columbia, Canada V8L 5X2 FAX: (250) 655- 5811 CHAIN OF CUSTODY AXYS CLIENT#: REPORTTO: INVOICETO: % Company Add" s ADE M F iv.U o flfe , Compariy \ W(%raA JDli/ko* S Addre:'s IHo0 Wpa-Ida uJou ^ -Sn 4 W A 5 , uz W a a I" ~f4~s>o'z>_____ ConM X X d iW o < > Phone 5 S3 S l1^ Contact (^^iurits Yftw/t* Phor e ' 6?It? 9 o l ^ # 7 - FAX S 6> E-mail 9^ FAx E-mttii Toi 34o i Project Name/Number: Sampler's Name: ?. ArX J Signature: ANALYSIS REQUESTED >A <o Client Sample Identification PAL-vjrr"ic>\- vipA- tin.c, T>M--Sw-T 2.oj^ PvPA-mui >Au-JvJ "TjoJ -ovPa-X'X pAt-rw-TM A-IIXO DA. *s v i-rra i'-o v p a - Pav -w- T<*ofo-VjPA" filT W i d r t - T W - W A ' rux 'Dav."Sw"TS - p a -R.ix PAt_-S\J "T^CI^- Du(>A--12120 Matrix w Sampling Sampling Date Time iT-f*Jzm. S' Ho V. I D II-fO ___ s____ fM -5*o n-.aa- Container Type/No. 1L 1 I f -10 <2 r lf ti /<>'./> N/ __ s k ___ Iz - Hf > Preservative Y /N -tJ * Y V/ AXYS Lab ID Lab use only * --C! - lx " 13 ~/4 V/ nnquished by (signature) Date Remarks / Type Of Preservative ^ p :e Time Date H - h e d - IT ._____Time / 0 ' L>S Received by (Signature) Date______________________Time ___________________ copy f V vAwk A fe s v lk Temp C cs> w e ll 3 Custody Seal # Seal Intact Sample Tags aw Sample Receipt Cooler Y /N Y /N m Page 14 of 307 www.axysanalytical.com Expanded Service International Air Waybill Express Not all services and options are available to all destinations. Company 5a Express Package Service Address . Address <' U ^ ________ _______________ , ^ i i. i. K i j Hi ... Dept/Roor C ity .0 ________ Province t i 1. CANADA Postai Code l O FFeeddEExx IInntteerrnnaattiiional Priority a P I FedEx International Erst . 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Postal Code "Q1 Total Packages Shipper's Load arid Cckjnt/SLAC Total W e ig ht. , a1*' A kg dim J ? >(b / id / A yf\xoA, p f in. cm 1 O HOLD at FedEx Location 3Q SATURDAY Delivery Ayaiiabfe to select locations Available for FedEx International Priority only Dogs th is ship m e nt contain dangerous goods? One box m ust be chBckad. __ _______ N o 4 Q Yes 8 H Yes As por attached 1-- 1 Shipper's Declaration Shippel's Declaration no t required B f l D,Y 1-- 1 tCB 9, UN 1845_ Dangerous Goods ^including D ry Ice) cannot be shipped in FedEx packaging. CA[___j Cargo Aircraft Only 8 Broker Selection Avllenle for FedEx International Priority and FedEx international Economy only 4 0 1 1international BrokerSelect *-- i Tospecify a brokerotherthan FedEx Broker's Name City/State/Country ZfP/Posts! Code ',,r .. 1?ECHI| il D ' Pilone - ,. C om m odity D escriptio n REQUIRED A V , / A ' T . 1 -- ->- ,iJ' . i : iH arm onizedjC odej? A C ou n try of M anufacture V alue fo r C ustom s REQUIRED S f j . Q O USA " V a oo 9a Payment B ill transportation charges to: :, , r t--------- Enter FedEx A c c t No. o r C redit Card No. b e l o w , ____ 1a' S ender Acct.No.in 2 3 I, R ecipient Soctkir t wijbecije-j. '-- -- ' Perty '4 i C re d it -- Card 5[___j Cash /Cheque / CreditCardExp. Date 9b i Payment B ill Customs charges to: J ___ Enter FedEx A c c t No. below. 2 P RecPe,lt 3 D K ALL shipments can be subject to Customs charges, whi.ch-FedEx does n o t estimate prior to clearance. ' 5 0 Cash/Cheque Canada Export Declaration/B13A: [ | No B13Arequired [| Manual B13Aattached [ | Bl3ASummaryfl8porting Auth.lD/FomrlD# Total Declared Value fo r Carriage /() 'A 3 j~~~] B13Afiledelectronically Total Declared Value ; for Customs Specify Currency OriB.nStation 1.0./ A A) i \ L, 1/ H " t V! r ; Handling Units Destination Station 1.0. i '! I1\ \ ! \ \ f A N ' v -i \ ; I V ' ' f f Total Volume URSA Routing \t l\ V \l / J n ; M'- ' !^ v Received A t-- . Reg.stop : 2| 1On-Call Stop S f i V " / f M i ) Audit 3J^J Drop Box nRt_ # . dA " sa ? ^ " Forms Attached: ]n ti __________ Del.Courier . W ' * ' Other Autre Non-Negotiable International Air Waybill 1934--2002 Federal Express Corporation Total. 10 Required Signature - e ??i,'? Ajt;y i!?Vbi!1constitutes your agreement to the Conditions of Contract on the back of this Air Waybill. Certain international treaties, including the Warsaw Convention, may applyto this shipment and limit our liabilityfor/aiiage, loss, or delay, as described Inthe Conditions of Contract Sender's Signature / / // iI fJ '/ / AJ / A / if J H .. Date Executed ,, r fa /L n Received aboveshipment in good order and condition. We agree to payall charges, including Customs duties and rnYocaeapplicable, and we agreeto the Conditions of Contract as statedonthe reverseside of the Recipient's Copy. FedEx 35S Slfl4 7403 Tracking Number Rev. Date 2/02 1 4 4 4 1 Part #157683 .'I... Il W j| 1994-2 PRINTED 00 IN 2 FedEx U.S.A. DS5 OREL, m Page 15 o f 3(l)7 www.axysanalytical.com Waybill : Date Shipped: AXYS Client & Contract # Project Number: Login Number: AXYS Analytical Services Ltd SAMPLE RECEIVING RECORD (^ e s)/No 1 2 -D E C -1 2 4731-Weston Solutions Waybill#: 835521847903 Date/Time Received: 1 4 -D E C -1 2 0 9 :1 5 Receipt No: W B 14214 Received By: KSTADLER Axys Sample IP's: L - \ Matrix Type: 5x w a te r \ Log in by: Signature: T V 1 A U I A z i a / / V x > Condition of Shipping Container: | Temperature upon Receipt: 4.7 Celcius shipped with w et ice, ice melted, no temp blank Thermometer ID: 3266 Custody Seals: Shipping Containers Yes l(fro) Samples Yes (N o ) Intact Y e s /N o Intact Y e s /N o Seal Numbers Y e s /N o Seal Numbers Y e s /No Chain of Custody or Documents: Sample ID's Collection Location Date & Time Collection Collector's Name Tracking Report/Packing List: Y e s (N o , Sample Tag Numbers Yes ^No^ Sample Type (YI)/No Preservative Added Preservation Requested Sample Tags Sample Labels Sample Labels Cross Referenced to COC Sample Tags Cross Referenced to Sample Labels Sample Tags Cross Referenced to COC Y e s f/iio ) (fe s jN o (Y e s )N o Yes /No Yes /No Information Agrees Information Agrees Information Agrees Comments: T D C ^ h>A I - s v u - i^o^OELi - K P A d i : I iq U b < 4 - S / M . - S > \ a ) - F L O E U - O - J Z Ll i q ____ (^Yes)No Yes /No Yes /No X Cu ( M v e w H % (lw \p \e I 0 0 q 'CV- VA Q S ' - 'tX V n - R s O S L l ' " 1 - \ ? Vr \ai Action Taken: Page 16 of 307 www.axysanalytical.com AXYS Analytical Services Ltd SAMPLE RECEIVING RECORD Waybill : Date Shipped: AXYS Client & Contract# Project Number: Login Number: (^Yes^/No 12-D E C -1 2 4731-Weston Solutions Waybill#: 8355218477903 Date/Time Received: 14-D E C -1 2 10:55 Receipt No: W B14215 Received By: KSTADLER Axys Sample IP's: L Q 8 ^ 8 ^ Matrix Type: 9 w a te r *~ Id? Log In by: ^ T T L - M T ' i T ^- t ________________________________________ Signature:1 E B 3 A A z v 2 w . Condition of Shipping Container: | Temperature upon Receipt: 2.1 Celcius shipped w ith wet ice, melted, temp blank present Thermometer ID: 3265 Custody Seals: Shipping Containers Yes m o Samples Yes Intact Yes /No Intact Yes /No Seal Numbers Yes /No Seal Numbers Yes /No Chain of Custody or Documents: Sample ID's Collection Location Date & Time Collection Collector's Name Tracking Report/Packing List: Sample Tag Numbers Sample Type Preservative Added Preservation Requested Sample Tags Sample Labels Sample Labels Cross Referenced to COC Sample Tags Cross Referenced to Sample Labels Sample Tags Cross Referenced to COC Yes(/No) (es)/No (Yes)lNo Yes /No Yes /No Information Agrees Information Agrees Information Agrees (Y f/N p Yes /No Yes /No Comments: Y' 1 lo io d P i i m - f \oX>el \ "=> CYv\rxC-;t`r f s lio i VvH M P .H . { Yn\fv^ CDC i no*-1^-4 j(A Action Taken: Page 17 of 307 www.axysanalytical.com K AXYS AXYS Analytical Services Ltd. Login C hain o f C u sto d y R epo rt (In 0 1 ) Dec. 1 7 ,2 0 1 2 10:05 AM L o g in N u m b e r : L18989 A ccount: 4731 W eston Solutions P roject: A x y s ID ve rs u s C lie n t S am p le Id en tificatio n L18989-1 DAL-SW-R02L3-DUPA-121116 16-NOV-12 13:20 ' Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY L18989-2 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-R15L1 -DU PA-121117 17-NOV-12 12:25 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY L18989-3 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-R10L3-DUPA-121119 19-NOV-12 11:00 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY L18989-4 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-R05L1 -1-121119 19-NOV-12 16:25 Storage: W1C2, 2A EDataDeliv D.Package Aqueous Aqueous ANY PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT /7 ~ o -s* Page: 1 of 4 Received 14-DEC-12 Due PR 1 1 L plastic 14-DEC-12 USD USD USD USD USD 1 1 L plastic 14-DEC-12 USD USD USD USD USD 1 1 L plastic 14-DEC-12 USD USD USD USD USD 1 1 L plastic USD USD USD USD USD Page 18 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. AXYS Login C hain o f C u sto d y R e p o rt (In 01) Dec, 1 7 ,2 0 1 2 10:05 AM Login N um ber: L18989 A ccount: 4731 W eston Solutions P ro ject: A x y s ID ve rs u s C lie n t S am p le Id en tificatio n L18989-5 DAL-SW-R06L2-DUPA-121120 20-NOV-12 16:30 Storage: WIC2, 2A EDataDeliv D. Package Aqueous Aqueous ANY L18989-6 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-T101-DUPA-121203 03-DEC-12 08:40 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY L18989-7 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-T202-DUPA-121203 03-DEC-12 09:10 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY L 1 8989-8 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-T303-DUPA-121203 03-DEC-12 11:50 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT Page: 2 of 4 Received 14-DEC-12 Due PR 1 1 L plastic 14-DEC-12 USD USD USD USD USD 1 1 L plastic 14-DEC-12 USD USD USD USD USD 1 1 L plastic 14-DEC-12 USD USD USD USD USD 1 1 L plastic USD USD USD USD USD Page 19 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. AXYS Login C hain o f C u s to d y R e p o rt (In 01) Dec. 1 7 ,2 0 1 2 10:05 AM Login N um ber: L18989 A ccount: 4731 W eston Solutions P roject: A xys ID versu s C lie n t S am p le Id e n tific a tio n L18989-9 DAL-SW-T404-DUPA-121203 03-DEC-12 14:50 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY L18989-10 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-T505-DU PA-121204 04-DEC-12 11:22 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY L18989-11 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-T606-DUPA-121204 04-DEC-12 12:55 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY L18989-12 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-T707-DUPA-121204 04-DEC-12 15:10 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT Page: 3 of 4 Received 14-DEC-12 Due PR 1 1 L plastic 14-DEC-12 USD USD USD USD USD ' 1 1 L plastic 14-DEC-12 USD USD USD USD USD 1 1 L plastic 14-DEC-12 USD USD USD USD USD 1 1 L plastic USD USD USD USD USD Page 20 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. $ )][(& AXYS Login C hain o f C u sto d y R ep o rt (In 0 1 ) Dec. 1 7 , 2 0 1 2 10:05 AM L o g in N u m b e r: L18989 A c c o u n t: 4731 Weston Solutions P roject: A x y s ID v e rs u s C lie n t S am p le Id e n tific a tio n L18989-13 DAL-SW-T808-DU PA-121205 05-DEC-12 10:10 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY L18989-14 PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT DAL-SW-T909-DUPA-121205 05-DEC-12 12:45 Storage: WIC2, 2A EDataDeliv D.Package Aqueous Aqueous ANY PFC EDD PFOS DATA PKG FC060 FC060.PS SAMPLE RECEIPT Page: 4 of 4 Received 14-DEC-12 Due PR 1 1 L plastic 14-DEC-12 USD USD USD USD USD 1 1 L plastic USD USD USD USD USD Page 21 of 307 www.axysanalytical.com Candice Navaroli From; Young, Charles T. [Charles.Young@WestonSolutions.com] S e n t: Friday, December 14, 2012 12:56 PM FILING To: Candice Navaroli; Smith, Martin; McLoughlin, Rachel; Kesari, Jai S u b je c t: RE: Shipment of Tennessee River SW samples for Thursday 12/12/2o12 arrival at AXYS Candice: Thanks for calling these to our attention. The last string is a date code of the form YYMMDD so the bottle ID (with the character in red denoting day of the week in your listing below) has the full sample ID for your login and reporting purposes. Please make the appropriate additions on the chain of custody (COC) form for these samples by inserting the final character from each of the sample bottles For the last sample, please correct the entry on the COC to match that recorded on the sample bottle by a single strike through DUPA and replacing with the number 1. Thanks, Charlie Charles T. Young Technical Director Weston Solutions, Inc. 1400 W eston Way (5-2) West Chester, PA 19380 (61.0) 701-3787 cell (610) 952-1093 fax (610) 701-3401 charles.young@ w esL onsolulions.com From: Candice Navaroli [mailto:cnavaroli@axys.com] Sent: Friday, December 14, 2012 3:25 PM To: Smith, Martin; McLoughlin, Rachel; Kesari, Jai Cc: Young, Charles T. Subject: RE: Shipment of Tennessee River SW samples for Thursday 12/12/2ol2 arrival at AXYS Hi Jai and Charles, There are a couple of sample label discrepancies that we need to have resolved before we can move forward with the logging in of your samples. There are several samples where the sample label on the bottle does not match the client ID listed on the chain of custody form. Please advise as to which one we should use. Sample Bottle ID Chain of Custody ID DAL-SW-T101-DUPA-12120 3 DAL-SW-T202-DUPA-12120 3 DAL-SW-T303-DUPA-12120 3 DAL-SW-T404-DUPA-12120 3 DAL-SW-T505-DUPA-12120 4 DAL-SW-T6Q6-DUPA-12120 4 DAL-SW-T707-DUPA-12120 4 DAL-SW-T808-DUPA-12120 5 DAL-SW-T909-DUPA-12120 5 DAL-SW-R05L1-G-121119 DAL-SW-T 101 -DUPA-12120 DAL-SW-T202-DUPA-12120 DAL-SW-T303-DUPA-12120 DAL-SW-T404-DUPA-12120 DAL-SW-T505-DUPA-12120 DAL-SW-T606-DUPA-12120 DAL-SW-T707-DUPA-12120 DAL-SW-T808-DUPA-12120 DAL-SW-T909-DUPA-12120 DAL-SW-R05L1-DUPA-121119 Regards, Candice Candice Navaroli (B.Sc.) 17/12/2012 Page 22 of 307 www.axysanalytical.com Project Manager Tel.: 250-655-5800 Direct: 250-655-5839 Toll free: 1-888-373-0881 Ext.5839 Fax: 250-655-5811 Email: cnavaroli@axys.com Axys Analytical Services Ltd. 2045 Mills Road Sidney, BC. Canada, V8L 5X2 17/12/2012 Page 23 of 307 www.axysanalytical.com BATCH LIST PART ONE BATCH ID: WG42273 Method ID: M LA-060 R10.03 Method ID: Method ID: A nalyst: S tart D ate: 18-D ec-12 B a tch L is t A u th o r: SDHESI Checked By: . P ro je c t M anager: G lassw are Type: G lassw are Treatment: Rotovap Type: D e vin , C a n d ic e R e g u la r R e g u la r N/A C om m ents: Sample size - 500mL AXYS Analytical Services Ltd Duplicate required for 4731. Choose largest available sample. M atrix: S a m p ie size: M o istu res req u ired ? L ip id s req u ired ? E x tra c tio n S o lv e n t: FM S Program : Save FM S d iscard s? B iohazard Type: Aqueous, EFFLUENT final 500 m L iNO :NO SPE N /A N /A . Hum an Biohazard Tor 4501 p re v io u s W G 's a s s o c ia te d w ith s a m p le s : W G42264 PS Spiking: Samples, Batch Surrogate, Recovery Spiking details Contract # Sample 4501 L1B992-1 4501 L1B992-2 4731 L1B9B9-1 4731 L16969 2 ...... 4731 I 1BSBS-3 4731 L18939-4 4731 L1S9S9-5 4731 L18989-6 4731 L1B989-7 4731 L18989-8 4731 L18389-9 Wt C ontract it Sam ple 500 mL 500 mL 500 mL 5U :r:L 500 mL 600 ml 50CmL 500 mL 500 mL 500 mL 500 mL 4731 L18989-10 500 mL 4731 L18989-11 500 mL 4731 L18989-12 500 mL 4731 Li 8989-13 500 mL 4731 L18989-14 500 ml . |w t Product FC060 Sam ples required: L189S2-1. 2. L 6S6S-1. 2. 3. 4, S, 5, 7. 8. &10. I t , 12. 13, 14. WG42273-1QI. 102, 103, 104 Before Extraction: Surrogate 20uL F C 023A -S U R /^ After Extraction: Cleanup Surrogate N/A ;Recovery F.V. : # t 50uL FC009A-REC/6 4000 uL ( 'ISWW^ U-vial Instructions and Comments: PPROP SNAP W/ MS/MSD: 105 u L FC039A-AUT; not sam e as SPM AUT; second SAR is US/MSD PVCO Authentic. PSA-004 Rev 16, 16-Feb-2010 m Page i Rag24 of 307 www.axysanalytical.com BATCH LIST PART TWO B A T C H ID: W G 42273 Type and QC Samples Weight Analysis QC :required for: Authentic spiking: AXYS Analytical Services Ltd QC samples, Authentic Spiking and Comments Comments: Q C S a m p le s Type and Analysis QC Weight required for: Authentic spiking: Comments: W G 42 273-101 BLANK 5C0ML CANADIAN SPRINGS WATER (OR 1 L FOR 1 L SAMPLES) W G 42273-102 SPM 500ML CANADIAN SPRINGS WATER (OR 1 L FOR 1 L s a m =_c s : ;i60uL FC039B-AT/ & J - W G 4 2 2 7 3 - 1 0 3 (FOR 4731 )500ML DUP OF LI 8989-)^ WG42273-1D4 SAR i160uLFC 039B .A U T/ FSA-004 Rev 16, 16-Feb-2010 Page zBag*e 25 of 307 www.axysanalytical.com AXYS Analytical Services Ltd Sample Labeling Information: Batch ID: WG42273 Method: MLA-060 R10.03 Date: I ^ ^ 6 Analyst: Sample ID Sample Type and Description Original Labelling Contract #:_____4731 L18989-1 L18989-2 L18989-3 L18989-4 L18989-5 L18989-6 L18989-7 L18989-8 L18989-9 L18989-10 L18989-11 L18989-12 L18989-13 L18989-14 C AiM r- c-nt-DoT XSs AA D A X S X ^ O X L V T s AA - O-USX X cxA ro -A tA x CATUAr coiou r - i oOi Al-\ X A l - s k j - e i s x i - T xiX X - n w i t - 0 o<< A"i t a \ exV Q. (-(pjCs,f-- c & A o r l-6.se> l ' c f s / t 4_ cvi'i'U i^ A L ~ S k X - 3 - 1 0 t > U ? A - \ Z i l H t v7 D ixc ~Vt W i- c . .r-'( 2 - l i t , L to l o u r l ,5 t ) o r Vtc^. W ' U I.Nw tL ia v iT U . 14a u - S k M ^ o c x 1" O - ! z 111*4 -S v \y . > L u -^ c x ry i-H ix in \ ^ U v w ' r_ in c r l-tsy I'o fV i i. T ) A L.-SU 3 L X -T > U ? A - IX 11X d i j. t , r r mto orW &3 W VUoW W . t'A'-'i A f TL, T>Al7 S U j T x i o I ' 1X\x o .3 A Ao r tto U r les *> ( A X 4 `h' I l b A L - S U A ^ T X o z . - T > 0 > A - i x V x o a " iW '2c u U Vkl . 1 '^r-Afir.r Colt) i j f Wt?S [ 7& t^j\ X W ' L T > A j - S i x - ' T 3 o3 -1X 12,0 3 4-a Co 1cs4-_ l3(jOw~ 7o/,oi A W a s VL*M a p t f x . r T s C a j W X c (.>4 T )A L -S lu -" m o M -T X rl> A - lX tx o 3 C X l U ^ r \ C o lo u r l e s S i ^ w ' <L llA L '-'& k J -X S O ^ lD U X A - W iz d V txvi -Vx 7)^r 4~'cv W V <? teOe&i't- c U e o r le s s l 'CtJ. 4 'piXC f-Toiv 1.V -- ^ C-ii-itf' C o ( jr U $ 3 i <4t/i A- w r^ L . t> A L ^S u ) - " r 4 o A ' huW A -Sx ix o 4 T tA X - S k A T T D l - 'b u X A - n j x o t f ipwr" Vi t v' 1^ i c ^ (V*.,* / -- c o l o u r Us s cL W /V L- SlX - T fio $ V - > > 0 ^ A - IX IX O S ' "par Voo U V iZ- 1 t k a - r c c tf o o r W s U ,, ; 4 ivA-fW m L - S U J ' T - ^ q - D u P A - a D . n S - 1' 1 LIM S la b e l c o rre c t as per c lie n t B7 FWO-305 Rev 07, 18-Dec-2012 Reviewed by_ date c l i Page of Page 26 of307 www.axysanalytical.com AXYS A nalytical S ervices Ltd Sam ple Labeling Information: Batch ID: WG42273 Method: MLA-060 R10.03 Date: Analyst: - Contract #: Sample ID Sample Type and Description Original Labelling NONE WG42273-103 (DUP) Dup a J -. --^ 0 ^ 8 e] -- / ! C -L tc < jr C o lo u f tfS 1 Jt D A L - S ^ - T f . o k - 'b u ^ A - ' ^ z .o 4 O^r-L i w A -i-t i FW O-305 Rev 07, 18-Dec-2012 Reviewed by_ date LIM S la bel c o rre c t as p e r c lie n t IC-P a g e . mo f _______ Page 27 of 307 www.axysanalytical.com AXYS Analytical Services Ltd Sam ple Labeling Inform ation: Batch ID: W G 42273_________ Date: (PH Pscn- Analyst: & & L - - M ethod: MLA-060 R10.03 Sample ID Sample Type and Description Original Labelling Contract #:_____NONE WG42273-101 (BLANK} WG42273-102 (SPM) WG42273-104 (SAR) Proodt ns, \ lldvcn p " CJ~P-c*s~ C o l o o f I&&S , C o l o u r iesS , f>Viv j X > S u c fD ^ c i.le /A t k i ^ ^ 4 'c W o v e r - gi /" 0-Vv-i-t cPCvl^v. 'A O C j -i . :J U A * ^ rin .A j . i / y i.ACsk -- ^C? 3b / LIM S la bel c o rre c t as p e r c lie n t FWO-305 Rev 07, t8-Dec-2012 Reviewed by. date ^ Page D Page 28 of 307 www.axysanalytical.com AXYS A nalytical Services Ltd Upfront 1QQx dilution Particulate Y/N Sam ple W t #2 : Batch ID: WG42273 Analyst Date: ( Method: MLA-060 R10.03 S a m p le ID Container + Sam ple S am p le w t (g) {C o m p ositin g S LA -084 Container Container Container + Sam ple Balance #: 3.* ot~j ) Sam ple w t ___ Sam ple w t ___ Cu b s a m p le r e q u ir e d (S L A -084 (g) ) Bottle + cap + E m pty bottle Total sam ple Remaining sample sam ple Sam ple W t. (g) L 1 8989-1 L 18 9 8 9 -2 L 18989-3 L 18989-4 L 18 9 8 9 -5 L 18989-6 L 18989-7 L18 9 89-8 L 18 9 89-9 L 18989-10 L 18989-11 L 18989-12 L 1 8 9 8 9 -1 3 L 1 8 9 8 9 -1 4 L 18992-1 L18 9 92-2 W G 4 2 2 7 3 -1 01 (B L A N K ) W G 4 2 2 7 3 -1 02 (S P M ) E x tra c tio n Inform atio n: visible particulate: S tirrin g : S olven t used: D C M S tart D ate: S h a k in g : tim e: S olven t used: D C M D ate: tim e: P a rtic u la te (so xh let extractio n ): S olven t: S tart D ate: tim e: FWO-307 Rev 08, 26-Jul-2011 no visible particulate: volu m e: end D ate: # of shakes: fr. U* foZO .12 z x I.SB > 5 -2 .4 3 ' ioo .'" m i e , s" > 7 . ^ r ? ai l a . Y ? ? ,3 8 '7 .0 8 .3 ? > ? ? io 7t> S o i ) 7 7 1 0 1 8 .S A 12. 17. ^ -+9" fO Z b .^ ! 0 3 3 . 576 .0 8 1 1 3 . ii'H H fr /r./x S iq .ib 0 X jo o ^5X0 'Y 7 o ^ 5Do ^ f 'o 'O s^SToo A S?t? ^Sbo s. S o o ^5 3 0 ^0 S7>0 'v 5~b> --> -- y5 ~ c t. *5 5 - S '/ S '. A$- y y & 0 .2 \ y Sot. LO W -11 4 M .U O ,7 > b 2 0 .7 -o W .V f y y y y y y y ^2k3< b iT -lS $0 8 . 0 4 y y y y y kiS 'D l . L z S T o |. kj m L A nalyst: tim e: vo lu m e: _mL A nalyst: volu m e: end D ate: Reviewed by date m L A nalyst: tim e: li Page 2 -yA of (r; Page 29 of 307 www.axysanalytical.com AXYS A nalytical S ervices Ltd Sam ple W t #2 : Batch ID: WG42273 Analyst ti)' Date: * Method: m la -060 R1 0 .0 3 S a m p le ID Container + Sample S a m p le w t (g) (C o m p o sitin g S LA -084 Container C o n ta in e r + Sample C o n ta in e r Balance #: ) Sample wt__ Sample wt__ S u b s a m p le required Bottle + cap + sample S LA -084 (at Empty Total bottle sample Remaining sam ple Sample Wt. (g) TJ C ID CL IS Q -< WG42273-103 (DUP) WG42273-104 (SAR) Co i E x tra c tio n In fo rm a tio n : visible particulate: Stirring: Solvent used: DCM Start Date: time: Shaking: Solvent used: DCM Date: time: Particulate (soxhlet extraction): Solvent: Start Date: time: FW O-307 Rev 08. 26-Jui-2011 no visible particulate: volume: end Date: # of shakes: mL Analyst: time: volume: volume: end Date: mL Analyst: time: Reviewed by date _mL Analyst: Page ; Page 30-ef 307 www.axysanalytical.com AXYS Analytical Services Ltd Spiking Sheet: Batch ID: WG42273 S LA-017 Sample ID Before extraction , Surr. ID: t0Q3A-S(li Date: SdE ^ iS Voi.: QO uL Syrinae#: 571IQ Ampoule: 5t Analyst: L18989-1 L18989-2 L18989-3 L18989-4 L18989-5 L18989-6 L18989-7 L18989-8 L18989-9 L18989-10 L18989-11 L18989-12 L18989-13 L18989-14 L18992-1 L18992-2 WG42273-101 (BLANK) WG42273-102 (SPM) S am p le ID Time Witness IQI5 iai6 tai IQ IC iQR IDlF \W ' am `a m \M m ii a \i (Qiq ia?^ IQq m c\ iaao 29-0 itAi j CM StbA JAM Alia J6 M J6H JAM J CM Ja M JAM JA M Ja m Si M Ja m icM Ac M JcM Authentic Std: Date; i$T)&C I Voi.: U 6 Syringe#: S i o l Ampoule#: _____ Analyst: D' Time Method: MLA-060 R10.03 Witness name/ initials: J a h u D Before extraction Surr. ID: Date: Voi.: uL Syrinqe#: Ampoule: Analyst: Cleanup Std. Surr.: Date: Voi.: uL Syringe#: Ampoule: Start time: Fin. time: Time Witness Analyst: `S iM Cleanup Std. Surr.: Date: Voi.: pL Svrinae#: Ampoule: Start time: Fin. time: Analyst: Recovery Recovery Surr.:cooAA-7&-A Surr.: Date: i &"!>CFZ- Date: Voi.: To uL Vol.: F.V.: Mte?o uL F.V.: Syrinqe#: -TT' Syrinqe#: Ampoule: t-x. Ampoule: Start time: <LZo Start time: Fin. time: srzM Fin. time: Analyst: L b ^ Analyst: J y y / y y y y y y y y y y y I ml Witness Authentic Std:_ Date: Voi.: ' Syringe#:. Ampoule#:_ Analyst: Tim e ml Witness y y y Authentic Std:_ Date: Voi.:. Syringe#: Ampoule#:_ _____ Analyst: T im e ml Witness uL uL ivfeu - loism tv fo M a a a ? ~io^ iSAV iai5 JcH J CM FWO-310 Rev 08, 16-Feb-2010 Reviewed by_ ( r o n date c i" pag, T A F ig e 3TT307 www.axysanalytical.com AXYS A nalytical S ervices Ltd Spikin g Sheet: B a tc h ID : WG42273_________________ M e t h o d: mla-060 R10.03 / ____________________ S L A - 0 1 7 ____________________________________W it n e s s n a m e / i n i t i a l s : k c( n U \f f / T L a A Sample ID Before extraction Suit. ID: Date: li'O t R Voi.: 'k uL Svrinqe#: Sl f 2 Amoouie: Y Analyst: t!> C --- 1 Before extraction Surr. ID: Date: Voi.: Svrinqe#: Ampoule: uL Analyst: Cleanup Std. Surr.: Date: Voi.: uL Svrinae#: Ampoule: Start time: Fin. time: Cleanup Std. Surr.: Date: Voi.: uL Svrinqe#: Ampoule: Start time: Fin. time: WG42273-103 (DUP) WG42273-104 (SAR) Time iQ Q a I1A A ^ Witness jc h UM Time Witness Analyst: Analyst: Recovery Surr.iR^to^A-Pee/ni Date: I1?T>c4 s2Vol.: -V--> uL F.V.: 4 c o uL Svrinqe#: Ampoule: I'LStart time: Fin. time: i 5 v C Analyst: FFW y Recovery Surr.: Date: Vol.: F.V.: Svrinqe#: Ampoule: Start time: Fin. time: Analyst: uL uL Sample ID Authentic Std: Date: Vol.: Svrinqe#: Ampoule#: Analyst: Time - uL Witness Authentic Std: Date Vol. Syringe# Ampoule# Analyst: Time ML Witness Authentic Std: Date Vol. Syringe# Ampoule# Analvst: Time PL Witness FWO-310 Rev 08, 16-Feb-2010 Reviewed by ^ 0 ^ - -- dateA ^ ? ' "" ' Page --s ui r / P age 3 2 ot307 www.axysanalytical.com LC Methods Worksheet Workgroup: WG42273 Sa m p le Preparation and Extractio n : Analyst: Reagent Lot# V o l- T im e mL VoL mL Tim e 50% formic acid 0.05 M KOH in MeOH 3% acetic acid 0.3% (NHjOH)/ 99% MeOH Reagent water ty p e :.............. Carbon (50 mg } acetonitrile (CH>CN) Formic acid cone, NHsOH cone. EDTA 0.14M Na2H P 04 buffer pH 2 HCI cone. ammonium acetate buffer 1 M, pH 5.0 ammonium acetate buffer 1 M, pH 6.5 ammonium acetate buffer 1 M, pH 4.0 Phosphate buffer 1 M, pH 6 .Q ' enzyme figlucoronidase/sulfatase enzyme ft- gtucoronidase 5% NHjOH in water H20 type 5% borate buffer pH 9 0.012 g/mL FMOC-Ci Acidified water with HCi ipH ) 3:1 MtBE:MeOH Sodium acetate buffer 0.5M, pH 4 10% acetate buffer pH 4 EOC.HCI 140mM KOH in 80:20 MeOH: Water MeOH A X YS Analytical Services Ltd __ Method: m l a -060 R10.Q3 Date: Fraction: Vol. Tim e mL S o n ic a tio n Analyst: Date: Time: fftimes: Extract pH check/adjust 1-P H Date; Time: (O.S-'J-.C l & D G c 52 f Z _Q Q Anaiyst: 2. pH i_ M anifold # rp 2 , P 3 A n a lyst j c -- Date: r 3 t i t r ^ i 2 Sample ID Position L18989-1 L 18989-2 j/ \ I L 18989-3 L 18989-4 V3 j #filter units Shake/ tipper Analyst: Date: Time: fftimes: D erivatization Anaiyst: Date: Start time: End time: Date: Time: Analyst: 3. pH Date: Time: Analyst: M iiiipore: A n a lyst:^ ^ Date: I Bi>C C\~z. Time: II 5~b F iltra tio n : In cu b a tio n : Analyst: Date: Start time: End time: L18989-5 L18989-6 L18989-7 L18989-8 L18989-9 L18989-10 L18989-11 3 }-; ^ <( 7 (o > / 1 X. { O r f L18989-12 /Z L18989-13 L18989-14 3 \4 L18992-1 L18992-2 T ) '7 \J '^ WG42273-101 (BLANK) \ i f S' C WG42273-102 (SPM) WG42273-103 (DUP) WG42 273-104 (SAR) i ^ V i t ;.......... Anaiyst: Date: Time: FWO-333 Rev 04, 26-Oct-2011 Reviewed by: 6 O c - Date: f C: Z Q --, Page H of C> Page 33 of 307 www.axysanalytical.com A X Y S Analytical Services Ltd LC M ethods C leanup W o rk s h e e t W o r k g r o u p : WG42273_____ M e th o d : m l a -060 r io .Q3 ig:S P E P r e -E lu tio n / C o n d itio n ir Date: f 1 2- A nalyst: t-TX- SPE type: S C w u/A-X Lot#: Reagent Lot VoL Tim e # mL 0.1 M Formic acid 0.3% NH4OH/ 99% MeOH 1: MeOH I'S-z. l03c> i2 .z .r 2: MeOH Reagent water type: Acidified water H ,0 Type (with HCI) pH DCM 2:30:68 Formic acid: Acetonitrile:MtBE W ash: / Load Sample: J Reagent Lot # Reagent water $43 type: C S fftn .. is 50% 0,1 M Formic acid/ 50% MeOH 0.1 M Formic add )5 L VoL mL ' s~ 0.3% basic (NH4 0H )/ 99% CS water Time iSZ'> 20% MeOH/ 80% 0.1 M Formic acid Acidified water HjOType (with HCI) pH E lu tion : Reagent MeOH 0.3% NHsOH/ 99% MeOH Carbon (optional) 1:1 Acetone:MeOH 2 % Formic acid in MeOH Lot # Acetonitrile (CH3CN) Hexane 2:30:68 Formic add: Acetonitrile: MtBE 2% NH4OH in water H20 Type 0.1 M HCI/ 5% MeOH CM Hexane 1: Acetonitrile (C H 3 CN) 2: Acetonitrile (C H 3 CN) 0.1 M H C I/5% MeOH MtBE 10:20:70 NH4OH: MeOH: MtBE Acetonitrile (CH3CN) 5% MeOH/ 95% water H20 Type 2% NH4OH in water. H ,0 Type Hexane MtBE CM 10:20:70 NH4 OH: MeOH:MtBE 1:1 OCM;MeOH 3:1 MtBE: MeOH Ethyl acetate 9:1 MtBE: MeOH Fraction: -- A nalyst: *-- Voi. Time mL is 'S ? R e c o n s titu te fo r jjviai: Date: A n a ly s t: / / / // / Reagent 0.1% Formic acid buffer Lot Voi. # rpL / / Tim e 1: MeOH 2: MeOH / // / / 3: MeOH /./ 0.3% NH4OH/99% / MeOH / / 70:30 MeOH: wafr H jO T vo e // / 1:1 MeOH: 0./% acetate buffer / 1 : 1 CHjCH' Ammonsurfi acetate, Acetic acid buffer 1:1 0,1% Acetic acid in CH3|6 n :Water HjO "fype 1:1 tf.2% Acetic acid in QH3CN:Water H2 0 Type Ragent water type: 0.5M Sodium Acetate Buffer pH 4 1:1 Acetonitrile: Acetone 5% Acetonitrile in water H20 Type MeOH 1:1 Acetonitrile: Acetone 2 % Formic acid in MtBE M e O H = M e th a n o l; M IB E = M e th y l-t-b u ty l ether; D C M = D ic h lo ro m e lh a n e ; M e C N = C H 3C N = A c e to n itrile ; N H 4O H = A m m o n iu m h y d ro x id e (3 0 % a q u e o u s u n le s s o th e rw is e s p e c .); C S = C a n a d ia n S p rin g s FWO-334 Rev 03, 18-Sep-2012 Reviewed by; Er>^- Date; { Page 34 of 307 www.axysanalytical.com Notes: Batch ID: WG42273 AXYS A nalytical S ervices Ltd Method: m la -060 R10.03 D ate: A n a ly s t Fraction N otes; Reviewed by date^ FWO-314 Rev 04, 13-May-2008 Page Page35"of 307 www.axysanalytical.com BATCH LIST PART ONE BATCH ID; WG42428 M ethod ID: M LA-060 R10.03 M etho d ID: M ethod ID: A n a ly s t : .................. S tart D ate: 1 4 -J a n -1 3 C om m ents: Sam ple size - 500mL B a tc h L i s t A u t h o r : ................. S D H E S I ................................ Checked By: ............................. P ro je c t M anaqe r: G lassw are Type: G lassw are Treatm ent: R otovap Type: i i - ....................................... C a n d ice R egular R egular N/A AXYS Analytical Services Ltd M atrix: S a m p le size: M o istu res re q u ired ? L ip id s re q u ire d ? Extraction Solvent: FMS Program : Save PMS discards? Aqueous 500 mL NO NO :s p e N/A N/A B io h a za rd T ype: None p re v io u s W G 's a s s o c ia te d w ith s a m p le s : W G 4 2264..P S , W G 4 2273_O R iG Contract # Sample Wt Contract # Sample 4731 LI 6989-1 R 590 mL 4731 L18989-2 R 500 mL 4731 L 18989-3 R 500 mL 4731 L18989-4 R 500 mL .....4731 ...... L18989-5 R 500 mL 4731 L18989-6 R 500 mL 4731 L18989-7 R 500 mL 4731 L18989-8 R 500 mL 4731 L18909-9 R 500 mL 4731 L18989-10 R 500 mL 4731 L1B9B9-12 R 500 mL 4731 L18989-1 3 R 500 mL 4731 L18989-14 R 500 mL Wt www.axysanalytical.com BATCH U ST PART TWO B A TC H ID: W G 42428 Type and QC Samples Weight Analysis QC required for: R'rCOOQ Authentic spiking: WG4242S-101 BLANK 500M l CANADIAN SPRINGS WATER (OR 1 L FOR 1 L SAMPLES) AXYS Analytical Services Ltd QC samples, Authentic Spiking and Comments Comments: Type and Analysis QC QC Samples Weight required for: Authentic spiking: Comments: .... .............: ...... ....... ................ HFCS63 160uL FC0398-AUT/ b T .................................. * * ......................................... WG42428-102 SPM 5 0 0 M L C A N A D IA N S P R IN G S W A T E R (O R 1 L F O R 1 l SAM PLES) W G 4 2 4 2 8 - 1 03 SAR SAR ........................... :............................ - - ................. RFCG0 ' 1 6 0 u L FC039B-AUT/ D ! ' 7 7 7 1 ..7 ' ^ V ^ ' 7 * . ' S. 7 7 ....... ................ ..................... ...... ..... ' ' ' I .............................. .. ............................. ........................................................................................ ............................. ........................................... FSA-004 Rev 16, 16-Feb-201G r....................................................................... page2pae 37 o f 307 www.axysanalytical.com AXYS Analytical Services Ltd S a m p le L a b e lin g In fo rm a tio n : Batch ID: W G 42428 Method: MLA-060 R10.03 _____ Date: iH Analyst: S a m p le ID Sam ple Type and D escription O riginal Labelling Contract #:_____4731 L18989-1 R L18989-2 R L18989-3 R L18989-4 R L18989-5 R L18989-6 R L18989-7 R L18989-8 R L18989-9 R L18989-10 R L18989-12 R L18989-13 R L18989-14 R WG42428-101 (BLANK) WG42428-102 (SPM) WG42428-103 (SAR) C,(Pa/ (cGsl&i lk/i VJJ rt i H. Y ui trni'L/liS .j-4 y f c i4(7/ . sltft/i-f uPfiUJ i/y W/ >rdl parti 0 lai-*- d m / ( A A pjj-, ticl u/y f h e partit cdaJ c ie a r 20U rl& y) }\nX djJ D A - L - S W - R o a L Y - D ^ P / A - Q 1116 D A L - S lJ - Q J S L A - W P A - f t M i* m - 5 k i - Q.\ o L 3 ~ DOPA d Z U i q " in e pa<r'ii r . J r l i 'c ZW rnb/Ucr? I h lJ fi W) m i - .TIaJ " 2 0 A L 3 - V U P A - I Z / I Z O A iC b paA V U f LQaj (Aoibcn I bi A VjJ Dfh p&r-f! tju\ c$jAV d m / rAor\$&) iki M K-/ -Fi An- na/tiicdXXx^ r\<?Gs rnAbd M D4 L - 5 W - T l o l ~ V v P A - 1X 1 7 -0 3 D A L - 51a ) - r i e >1 - D ir PA ~ a i Z o l D A L - SW ~ t Z o 3 - DO PA - 2 1 2 ? F A - r .\&&/ (dofli f t o j y vjJ P A L - 5 W - T HoH- V c P A - 121203 -Tihp. nartiicjAcJJ' d &&C cAor\juy> ^Cyi WJ D A L -5 U - T$o? -DUPA -IZiZOti po.rFl A-IoI a. d&D-r r c J / U /p wJ D A L - 5 LJ - T A c i T - D D P A - -iAA, Partic,y\/M^ rlpoz cA or o*Q r>oev) va/ P A ^ W - T ^ S - Q U P A - 121-20 5 4W pa.rtilA f t d @jP/ Coter Qjy> \\Q\y*1 VO D A L ' C U j - T 4 *3 - D o P A " i X l 7_0 5 -i'ifVP, atddXjL c o jy s cAo(\,p/y^ ho\jiA r .......................L i3 . L A . W i f . i l ........ r iQ.as fc v ij iS P M ^ Ccc/AxAUoj^ V n n A G A UACi^iS^ 3b 5 f y C u n a r iu ^ 5 p n r \ ^ o Vjak&O B a k c k iA - 1>b 5 ro : s .A e. FWO-305 Rev 07, 18-Dec-2012 Reviewed by date 3 LIMS label correct as per client Page 38 of 307 Upfront tOOx dilution Particulate Y/N www.axysanalytical.com AXYS Analytical Services Ltd Sam ple Wt #2 : Batch ID: WG42428 Analyst -3 Date: H -MfJ I? Bal ance #: Method: m l a -0 6 0 r i o .03 Sample ID Container + Sam ple Sample wt (g) (Compositing SLA-084 Container + Sam ple Container Container ) Sam ple wt__ Sam ple wt__ Ciubsample required SLA-084 (g) ) Bottle + Empty cap + bottle Total sam ple Remaining sample sam ple L18989-1 R L18989-2 R L18989-3 R L18989-4 R L18989-5 R L18989-6 R L18989-7 R L18989-8 R L18989-9 R L18989-10 R L18989-12 R L18989-13 R L18989-14 R WG42428-101 (BLANK) WG42428-102 (SPM) WG42428-103 (SAR) to33.io 499.- 534.U ^.\0\ 453. sas.oa 44 44 s a w 4 4 3 .aa 4 C U 4 4i3}.>) w a .q a SIS. 43 444, |C\ iOOk %.3g 515.3$ <1)3.40 441. 0 5 433.35 143. ta 4q4. VVS.4 19 444.40 4 4 5 4 4 455.44 43.3 0 to5 aa 1 . 10 s v a .ia 334.55 3 .1 1 54o M ---- 0 3 a 0 0 0 } 0 0 0 0 0 0 -- --" ---- Sam ple W t. (g) y Vi uir 1/ i XV8 X- r r V i y y r KJ NJ .-- . E x tra c tio n Inform atio n: visible particulate: Stirrina: Solvent used: DCM Start Date: time: Shaking: Solvent used: DCM Date: time: Particulate (soxhlet extraction): Solvent: Start Date: time: FWO-307 Rev 08, 26-Jui-2011 no visible particulate: volume: end Date: # of shakes: mL Analyst: time: volume: volume: end Date: mL Analyst: time: Reviewed bv 5 T C M - date mL Analyst: Pagea IC Page 39 of 307 www.axysanalytical.com AXYS Analytical Services Ltd Spiking Sheet: Batch ID: WG42428 Method: MLA-060 R1Q.03 S L A -0 17 Witness name/ initials: Y oM 6f '\ Sample ID Before extraction^ _ , Before extraction Sum ID: ' Surr. ID: Date:'it'"knAi% Date: Vol.: xo uL Voi.: pL Svrinqe#: Svrinqe#: Ampoule: Of Ampoule: Analyst: 1 CM- Analyst: Cleanup Std. Surr.: Date: Voi.: uL Svrinqe#: Ampoule: Start time: Fin. time: Cleanup Std. Surr.: Date: Voi.: uL Svrinqe#: Ampoule: Start time: Fin. time: L18989-1 R L18989-2 R L18989-3 R L18989-4 R L18989-5 R L18989-6 R L18989-7 R L18989-8 R L18989-9 R L18989-10 R L18989-12 R L18989-13 R L18989-14 R WG42428-101 (BLANK) WG42428-102 (SPM) WG42428-103 (SAR) Time fo*> f O M 'M S O '^ \D '- A 4 - 1O: l 2 10-2 Ut -Ah so', 1-4-0 ! 0-4-1 1 o i4 -| io-W Witness M ttx y-f*. '-fa. ' cL "Ma. 'fs* 4-ftx U -fa 44. H'ftX if * Time Witness Analyst: Analyst: Recovery Recovery Surr.: rC O o ^ k -g iC J f 1Surr.: Date: M-JAKWB Date: Voi.: a uL Voi.: F.V.: Ucce uL F.V.: Svrinqe#: JV/ty Svrinqe#: Ampoule: H Ampoule: Start time: N : 15 Start time: Fin. time: M '#7- Fin. time: uL uL Analyst: Analyst: M v-" s' v/ Sample ID yj(x, -- c i. $ Authentic Std:|V.oVaj&--AuT/p'j. Date Voi. f ho ML Syringe# s l Ampoule# Analyst: jz y i Time Witness .Li 7) gQLM-3. 4-fa. Authentic Std:__ Date: Voi.: Syringe#: _ Ampoule#:^ _____ Analyst: Time ML Witness Authentic Std: Date Voi. Syringe#: Ampoule#: _____ Analyst: Time .HL Witness FWO-310 Rev 08, 16-Feb-2010 Reviewed by_V i t W date Pa., 3 ,C C Page 40 of 307 www.axysanalytical.com A X Y S Analytical Services Ltd L C M e t h o d s W o r k s h e e t Workgroup: W G 4 2 4 2 8 ________ Method: MLA-060 R1Q.03 Sample Preparation and Extraction: Analyst: TCM____ Date: " J'PjXj'- Fraction: Reagent Lot# VoL Tim e mL 50% formic acid 0.05 M KOH in MeOH 3% acetic 3cid 0.3% (NHjOH)/ 99% MeOH Reagent water type: C . S . Carbon (50 mg } acetonitrile (CH3CN) Formic acid cone. NHiOH cone. EDTA 0.14 M Na,HPO< buffer pH 2 HCI cone. ammonium acetate buffer 1 M. pH 5.0 ammonium acetate buffer 1 M, pH 6.5 ammonium acetate buffer 1 M, pH 4.0 Phosphate buffer 1 M, pH 6.0 enzyme IV qiucoronidase/sulfaiase enzyme ft- giucoronidase 5% NHaOH in water H?G type 5% borate buffer pH 9 0.012 g/mL FMOC-CI Acidified water with HCi (pH ) 3:1 MLBE: MeOH Sodium acetate buffer 0.5M, pH 4 10% acetate buffer pH 4 EOC.HCI 140mM KOH in 80:20 MeOH: Water MeOH Val. Tim e mL VoL Tim e mL Sonication Analyst: Date: Tim e: #times: Shake/ tipper Analyst: Date: Time: #times: Derivatization Analyst: Date: Start time: End tim e: Millipore: Analyst: J c Date: | U Time: Qg:il$ Filtration: Analyst: a te : Time: Extract pH check/adjust 1'P H . S O . r Manifold # P S , PK Analyst 7 c m . ' Date: H - j/X N 'O Sample ID Position Date: f t Time: 0 q L18989-1 R L18989-2 R f li ( IS Analyst: "XCh\ 2. pH L18989-3 R \ __________________________IL L18989-4 R |) ^ ^ 3 Date: Tim e: Analyst: 3. pH Date: Time: Analyst: L18989-5 R L18989-6 R L18989-7 R L18989-8 R L18989-9 R L18989-10 R L18989-12 R L18989-13 R L18989-14 R 1 12 j i f \c l iS! (G o fi I IZ PQ J ] <3 IH WG42428-101 (BLANK) / (5 WG42428-102 (SPM) WG42428-103 (SAR) ---- incubation: Analyst: Date: Start lime: End time: #filter units FWO-333 Rev 04, 26-Oct-2011 Reviewed by: J Z m Date: l L! ' 3 P a g e ...V . o f <b___ Page 41 o f 307 www.axysanalytical.com AXYS Analytical Services Ltd LC M ethods Cleanup W o rksh e e t Workgroup: WG42428_____ Method: m l a - 0 6 0 r io .Q3 SPE P re -E lu tio n / C o n d itio n in g : Date: A na lyst: Sc M SPE tvoe: 150m A W /fti Lot#: 0 I V 3 __________________ Reagent Lot Vol. Tim e # mL 0.1 M Formic acid 5 IN ? 0.3% NHaOH/ 99% MeOH 9q? 5 mi 1: MeOH 2: MeOH Reagent water type: Acidified water H',0 Tvoe (with HCI) pH DCM 2:30:68 Formic acid: AcetonitrilerMtBE 2% NH4OH in water H?0 Type W ash: , Load S am ple: sx Reagent Reagent water type: C. 5 50% 0.1 M Formic acid/ 50% MeOH 0.1 M Formic acid 0.3% basic (NH4 OH)/ 99% CS water 20% MeOH/ 80% 0.1 M Formic acid Lot # %5 V ol. Time mL 9 5 W'-Oo Acidified water H2OTvpe (with HCt) pH 0.1 M HCl/ 5% MeOH E lution: Reagent MeOH 0.3% NH4OH/ 99% MeOH Carbon (optional) 1:1 Acetone:MeOH 2 % Formic acid in MeOH Lot # Acetonitrile (CH3 CN) Hexane 2:30:68 Formic acid; Acetonitrile: MtBE DCM Hexane 1: Acetonitrile (C H 3 CN) 2: Acetonitrile (CHjCN) 0.1 M HCI/ 5% MeOH MtBE 10:20:70 NH4OH: MeOH: MtBE Acetonitrile (CH3CN) 5%MeOH/ 95% water H30 Type 2% NH4 OH in water, H20 Type Hexane MtBE DCM 10:20:70 NH4OH: MeOH;MtBE 1:1 DCM:MeOH 3:1 MtBE: MeOH Ethyl acetate 9:1 MtBE: MeOH Fraction:_______ Analyst: JCM R e c o n s titu te fo r jjvial: Date: Analyst: V ol. Time mL 4 ^60 Reagent 0.1% Formic acid buffer 1: MeOH 2: MeOH 3: MeOH 0.3% NH3OH/ 99% MeOH 70:30 MeOH: water H O Type Lot Vol, # mL 1:1 MeOH: 0.1% acetate buffer 1:1 CH3CN: Ammonium acetate, Acetic acid buffer 1:1 0.1% Acetic acid in CH3CN:Water H20 Type 1:1 0.2% Acetic acid in CH3CN:Water H20 Type Reagent water type: Tim e 0.5M Sodium Acetate Buffer pH 4 1:1 Acetonitrile: Acetone 5% Acetonitrile in water H20 Type MeOH 1:1 Acetonitrile; Acetone 2 % Formic acid in MtBE MeOH= Methanol; MtBE= Methyl-t-butyl ether; DCM= Dichloromethane; MeCN= CH3CN= Acetonitrile: NH4OH= Ammonium hydroxide (30% aqueous unless otherwise spec.); CS= Canadian Springs FWO-334 Rev 03, 18-Sep-2012 Jt M Reviewed b y :__________ Date: Page 42 of 307 www.axysanalytical.com Notes: Batch ID: WG42428 AXYS Analytical Services Ltd Method: M LA-060 R 10.03 Date: Analyst Fraction Notes: JdM . Reviewed b y __________ date / V - J 4 t O - l 3 FWO-314 Rev 04, 13-May-2008 Page 43 of 307 www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D A L-SW -R02L3-D U PA-121116 Sample Collection: 16-Nov-2012 13:20 FALL 2012 TENNESSEE RIVER SAMPLING L18989-1 R Matrix: AQUEOUS Sample Size: 0.525 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 01:38:17 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 23 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 8.82 0.952 (L) 2.22 0.952 (L) 3.83 0.952 (L) 4.64 0.952 (L) 9.14 0.952 (L) U 0.952 (L) U 0.952 (L) U 0.952 (L) U 0.952 (L) 2.70 1.90 (L) U 1.90 (L) 7.59 1.90 (L) U 0.952 (L) 5:12 5:57 6:21 6:44 7:06 6:27 8:10 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L 18989-1_Form1 A_FC3G_015S23_SJ1552186.html; Workgroup: W G42428;DesignID: 1980 ] Page 44 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-1_Form1A_FC3G_015S23_SJ1552186.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D A L-SW -R02L3-D U PA-121116 Sample Collection: 16-Nov-2012 13:20 FALL 2012 TENNESSEE RIVER SAMPLING L18989-1 R Matrix: AQUEOUS Sample Size: 0.525 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 01:38:17 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 23 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 8.29 9.46 33.1 12.1 10.8 10.6 17.4 18.5 69.1 78.8 92.1 101 90.2 88.6 96.9 103 5:11 6:20 7:06 7:29 8:03 9:11 8:21 7:20 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-1_Form2_FC3G_015S23_SJ1552186.html; Workgroup: WG42428; Design ID: 1980 ] Page 45 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-1_Form2_FC3G_015S23_SJ1552186.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D A L-SW -R15L1 -D UPA-121117 Sample Collection: 17-Nov-2012 12:25 FALL 2012 TENNESSEE RIVER SAMPLING L18989-2 R Matrix: AQUEOUS Sample Size: 0.454 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 01:55:18 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 24 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 10.4 1.10 (L) 2.93 1.10 (L) 4.21 1.10 (L) 3.88 1.10 (L) 10.6 1.10 (L) U 1.10 (L) U 1.10 (L) U 1.10 (L) U 1.10 (L) 20.9 2.20 (L) U 2.20 (L) 22.0 2.20 (L) U 1.10 (L) 5:12 5:56 6:21 6:44 7:06 6:27 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-2_Form1A_FC3G_015S24_SJ1552187.html; Workgroup: W G42428;DesignID: 1980 ] Page 46 of 307 Page 1 of 1 (WG42428 -PFC_FC_LC_PFOA_L18989-2_Form1A_FC3G_015S24_SJ1552187.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D A L-SW -R15L1 -D UPA-121117 Sample Collection: 17-Nov-2012 12:25 FALL 2012 TENNESSEE RIVER SAMPLING L18989-2 R Matrix: AQUEOUS Sample Size: 0.454 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 01:55:18 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 24 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 9.68 9.56 35.0 13.2 12.0 12.0 17.6 19.4 80.7 79.7 97.2 110 99.8 100 97.8 108 5:12 6:21 7:06 7:29 7:59 9:11 8:21 7:20 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-2_Form2_FC3G_015S24_SJ1552187.html; Workgroup: WG42428; Design ID: 1980 ] Page 47 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-2_Form2_FC3G_015S24_SJ1552187.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D A L-SW -R10L3-D U PA-121119 Sample Collection: 19-Nov-2012 11:00 FALL 2012 TENNESSEE RIVER SAMPLING L18989-3 R Matrix: AQUEOUS Sample Size: 0.465 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 02:12:11 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 25 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 6.79 1.08 (L) 1.67 1.08 (L) 1.64 1.08 (L) 1.24 1.08 (L) 2.56 1.08 (L) U 1.08 (L) U 1.08 (L) U 1.08 (L) U 1.08 (L) U 2.15 (L) U 2.15 (L) 5.48 2.15 (L) U 1.08 (L) 5:15 5:57 6:21 6:41 7:06 8:10 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L 18989-3_Form1 A_FC3G_015S25_SJ1552188.html; Workgroup: W G42428;DesignID: 1980 ] Page 48 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-3_Form1A_FC3G_015S25_SJ1552188.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D A L-SW -R10L3-D U PA-121119 Sample Collection: 19-Nov-2012 11:00 FALL 2012 TENNESSEE RIVER SAMPLING L18989-3 R Matrix: AQUEOUS Sample Size: 0.465 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 02:12:11 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 25 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 8.67 9.47 32.8 11.7 10.1 10.4 17.3 17.0 72.3 78.9 91.2 97.9 84.5 86.6 96.3 94.4 5:12 6:21 7:06 7:29 8:03 9:11 8:21 7:20 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-3_Form2_FC3G_015S25_SJ1552188.html; Workgroup: WG42428; Design ID: 1980 ] Page 49 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-3_Form2_FC3G_015S25_SJ1552188.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -R05L1-1-121119 Sample Collection: 19-Nov-2012 16:25 FALL 2012 TENNESSEE RIVER SAMPLING L18989-4 R Matrix: AQUEOUS Sample Size: 0.443 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 02:29:12 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 26 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 75.5 1.13 (L) 17.0 1.13 (L) 66.1 1.13 (L) 64.7 1.13 (L) 210 1.13 (L) 2.01 1.13 (L) U 1.13 (L) U 1.13 (L) U 1.13 (L) 166 2.26 (L) 35.5 2.26 (L) 388 2.26 (L) 20.3 1.13 (L) 5:12 5:57 6:21 6:41 7:06 7:29 6:29 7:20 8:17 9:59 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-4_Form1A_FC3G_015S26_SJ1552189.html; Workgroup: W G42428;DesignID: 1980 ] Page 50 of 307 Page 1 of 1 (WG42428 -PFC_FC_LC_PFOA_L18989-4_Form1A_FC3G_015S26_SJ1552189.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -R05L1-1-121119 Sample Collection: 19-Nov-2012 16:25 FALL 2012 TENNESSEE RIVER SAMPLING L18989-4 R Matrix: AQUEOUS Sample Size: 0.443 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 02:29:12 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 26 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 9.67 9.05 32.1 12.0 11.1 11.3 18.4 20.3 80.6 75.4 89.1 99.9 92.8 94.1 102 113 5:11 6:21 7:06 7:29 7:59 9:11 8:17 7:20 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-4_Form2_FC3G_015S26_SJ1552189.html; Workgroup: WG42428; Design ID: 1980 ] Page 51 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-4_Form2_FC3G_015S26_SJ1552189.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D A L-SW -RO 6L2-D U PA-121120 Sample Collection: 20-Nov-2012 16:30 FALL 2012 TENNESSEE RIVER SAMPLING L18989-5 R Matrix: AQUEOUS Sample Size: 0.413 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 02:46:05 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 27 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 6.18 1.21 (L) 3.81 1.21 (L) 1.62 1.21 (L) U 1.21 (L) 2.75 1.21 (L) U 1.21 (L) U 1.21 (L) U 1.21 (L) U 1.21 (L) 2.69 2.42 (L) U 2.42 (L) 7.66 2.42 (L) U 1.21 (L) 5:14 5:55 6:21 7:06 6:29 8:14 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-5_Form1A_FC3G_015S27_SJ1552190.html; Workgroup: WG42428; Design ID: 1980 ] Page 52 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-5_Form1A_FC3G_015S27_SJ1552190.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D A L-SW -RO 6L2-D U PA-121120 Sample Collection: 20-Nov-2012 16:30 FALL 2012 TENNESSEE RIVER SAMPLING L18989-5 R Matrix: AQUEOUS Sample Size: 0.413 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 02:46:05 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 27 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 9.84 9.98 32.9 12.2 11.1 9.90 17.7 19.1 82.0 83.1 91.3 102 92.4 82.5 98.1 106 5:11 6:20 7:06 7:29 7:59 9:11 8:17 7:17 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-5_Form2_FC3G_015S27_SJ1552190.html; Workgroup: WG42428; Design ID: 1980 ] Page 53 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-5_Form2_FC3G_015S27_SJ1552190.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T101-DU PA-121203 Sample Collection: 03-Dec-2012 08:40 FALL 2012 TENNESSEE RIVER SAMPLING L18989-6 R Matrix: AQUEOUS Sample Size: 0.497 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 03:03:06 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 28 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 5.42 1.01 (L) 1.51 1.01 (L) 1.69 1.01 (L) 1.16 1.01 (L) 2.54 1.01 (L) U 1.01 (L) U 1.01 (L) U 1.01 (L) U 1.01 (L) U 2.01 (L) U 2.01 (L) 5.26 2.01 (L) U 1.01 (L) 5:11 5:57 6:21 6:41 7:06 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L 18989-6_Form1 A_FC3G_015S28_SJ1552191.html; Workgroup: W G42428;DesignID: 1980 ] Page 54 of 307 Page 1 of 1 (WG42428 -PFC_FC_LC_PFOA_L18989-6_Form1A_FC3G_015S28_SJ1552191.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T101-DU PA-121203 Sample Collection: 03-Dec-2012 08:40 FALL 2012 TENNESSEE RIVER SAMPLING L18989-6 R Matrix: AQUEOUS Sample Size: 0.497 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 03:03:06 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 28 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 9.18 9.42 33.4 12.8 11.3 10.0 17.9 17.7 76.5 78.5 92.7 106 94.3 83.5 99.2 98.1 5:09 6:21 7:06 7:29 7:59 9:09 8:17 7:17 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-6_Form2_FC3G_015S28_SJ1552191.html; Workgroup: WG42428; Design ID: 1980 ] Page 55 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-6_Form2_FC3G_015S28_SJ1552191.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T202-DU PA-121203 Sample Collection: 03-Dec-2012 09:10 FALL 2012 TENNESSEE RIVER SAMPLING L18989-7 R Matrix: AQUEOUS Sample Size: 0.492 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 03:19:59 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 29 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 8.61 1.02 (L) 3.08 1.02 (L) 4.93 1.02 (L) 6.01 1.02 (L) 10.5 1.02 (L) U 1.02 (L) U 1.02 (L) U 1.02 (L) U 1.02 (L) 3.83 2.03 (L) U 2.03 (L) 8.44 2.03 (L) U 1.02 (L) 5:14 5:58 6:22 6:44 7:06 6:31 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-7_Form1A_FC3G_015S29_SJ1552192.html; Workgroup: WG42428; Design ID: 1980 ] Page 56 of 307 Page 1 of 1 (WG42428 -PFC_FC_LC_PFOA_L18989-7_Form1A_FC3G_015S29_SJ1552192.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T202-DU PA-121203 Sample Collection: 03-Dec-2012 09:10 FALL 2012 TENNESSEE RIVER SAMPLING L18989-7 R Matrix: AQUEOUS Sample Size: 0.492 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 03:19:59 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 29 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 8.25 9.03 30.9 11.9 10.4 9.36 16.5 16.1 68.7 75.3 86.0 99.1 87.0 78.0 91.7 89.5 5:11 6:21 7:06 7:29 7:59 9:09 8:21 7:20 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-7_Form2_FC3G_015S29_SJ1552192.html; Workgroup: WG42428; Design ID: 1980 ] Page 57 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-7_Form2_FC3G_015S29_SJ1552192.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T303-DU PA-121203 Sample Collection: 03-Dec-2012 11:50 FALL 2012 TENNESSEE RIVER SAMPLING L18989-8 R Matrix: AQUEOUS Sample Size: 0.483 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 03:37:00 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 30 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 5.91 1.04 (L) 1.87 1.04 (L) 1.67 1.04 (L) 1.15 1.04 (L) 3.07 1.04 (L) U 1.04 (L) U 1.04 (L) U 1.04 (L) U 1.04 (L) U 2.07 (L) U 2.07 (L) 6.68 2.07 (L) U 1.04 (L) 5:14 5:55 6:21 6:44 7:06 8:14 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-8_Form1A_FC3G_015S30_SJ1552193.html; Workgroup: W G42428;DesignID: 1980 ] Page 58 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-8_Form1A_FC3G_015S30_SJ1552193.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T303-DU PA-121203 Sample Collection: 03-Dec-2012 11:50 FALL 2012 TENNESSEE RIVER SAMPLING L18989-8 R Matrix: AQUEOUS Sample Size: 0.483 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 03:37:00 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 30 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 8.99 9.77 33.2 12.6 10.7 10.4 18.0 18.3 74.9 81.4 92.3 105 89.1 87.0 100 102 5:12 6:21 7:06 7:29 7:59 9:11 8:21 7:20 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-8_Form2_FC3G_015S30_SJ1552193.html; Workgroup: WG42428; Design ID: 1980 ] Page 59 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-8_Form2_FC3G_015S30_SJ1552193.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T404-DU PA-121203 Sample Collection: 03-Dec-2012 14:50 FALL 2012 TENNESSEE RIVER SAMPLING L18989-9 R Matrix: AQUEOUS Sample Size: 0.422 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 03:54:01 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 31 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 6.09 1.18 (L) 1.21 1.18 (L) 1.58 1.18 (L) 1.28 1.18 (L) 2.53 1.18 (L) U 1.18 (L) U 1.18 (L) U 1.18 (L) U 1.18 (L) U 2.37 (L) U 2.37 (L) 6.30 2.37 (L) U 1.18 (L) 5:11 5:57 6:20 6:41 7:06 8:14 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L 18989-9_Form1 A_FC3G_015S31_SJ1552194.html; Workgroup: W G42428;DesignID: 1980 ] Page 60 of 307 Page 1 of 1 (WG42428 -PFC_FC_LC_PFOA_L18989-9_Form1A_FC3G_015S31_SJ1552194.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T404-DU PA-121203 Sample Collection: 03-Dec-2012 14:50 FALL 2012 TENNESSEE RIVER SAMPLING L18989-9 R Matrix: AQUEOUS Sample Size: 0.422 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 03:54:01 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 31 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 8.56 9.47 35.5 12.0 10.1 9.58 16.7 16.6 71.3 78.9 98.7 100 84.1 79.8 93.0 92.1 5:11 6:20 7:06 7:29 7:59 9:09 8:17 7:17 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-9_Form2_FC3G_015S31_SJ1552194.html; Workgroup: WG42428; Design ID: 1980 ] Page 61 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-9_Form2_FC3G_015S31_SJ1552194.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -T505-DU PA-121204 Sample Collection: 04-Dec-2012 11:22 FALL 2012 TENNESSEE RIVER SAMPLING L18989-10 R Matrix: AQUEOUS Sample Size: 0.449 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 04:10:54 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 32 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 6.14 1.11 (L) U 1.11 (L) 1.56 1.11 (L) 1.29 1.11 (L) 2.65 1.11 (L) U 1.11 (L) U 1.11 (L) U 1.11 (L) U 1.11 (L) U 2.23 (L) U 2.23 (L) 5.10 2.23 (L) U 1.11 (L) 5:16 6:22 6:44 7:06 8:07 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-10_Form1A_FC3G_015S32_SJ1552195.html; Workgroup: WG42428; Design ID: 1980 ] Page 62 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-10_Form1A_FC3G_015S32_SJ1552195.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -T505-DU PA-121204 Sample Collection: 04-Dec-2012 11:22 FALL 2012 TENNESSEE RIVER SAMPLING L18989-10 R Matrix: AQUEOUS Sample Size: 0.449 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 04:10:54 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 32 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 8.86 10.0 34.3 12.2 10.5 10.4 16.2 17.6 73.9 83.4 95.3 101 87.2 86.6 90.1 97.7 5:15 6:22 7:09 7:29 7:59 9:07 8:17 7:20 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-10_Form2_FC3G_015S32_SJ1552195.html; Workgroup: WG42428; Design ID: 1980 ] Page 63 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-10_Form2_FC3G_015S32_SJ1552195.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -T606-DU PA-121204 Sample Collection: 04-Dec-2012 12:55 FALL 2012 TENNESSEE RIVER SAMPLING L18989-11 (A) Matrix: AQUEOUS Sample Size: 0.498 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 13-Dec-2012 Extraction Date: 18-Dec-2012 Instrument ID: LC MS/MS A nalysis Date: 18-Dec-2012 Time: 23:49:24 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC2G_455 S: 21 Injection Volume (uL): 15 Blank Data Filename: FC2G_455 S: 10 Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_455 S: 4 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 6.01 1.00 (L) 1.59 1.00 (L) 2.09 1.00 (L) B 2.11 1.00 (L) 3.62 1.00 (L) U 1.00 (L) U 1.00 (L) U 1.00 (L) U 1.00 (L) 2.14 2.01 (L) U 2.01 (L) 7.45 2.01 (L) U 1.00 (L) 5:09 5:59 6:21 6:42 7:04 6:30 8:03 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL; B = analyte found in sample and the associated blank. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-11_Form1A_FC2G_455S21_SJ1549333.html; Workgroup: WG42273; Design ID: 1980 ] Page 64 of 307 Page 1 of 1 (WG42273 -PFC_FC_LC_PFOA_L18989-11_Form1A_FC2G_455S21_SJ1549333.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -T606-DU PA-121204 Sample Collection: 04-Dec-2012 12:55 FALL 2012 TENNESSEE RIVER SAMPLING L18989-11 (A) Matrix: AQUEOUS Sample Size: 0.498 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 13-Dec-2012 Extraction Date: 18 -D e c-2 0 1 2 Instrument ID: LC MS/MS A nalysis Date: 18-Dec-2012 Time: 23:49:24 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC2G_455 S: 21 Injection Volume (uL): 15 Blank Data Filename: FC2G_455 S: 10 Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_455 S: 4 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 8.35 8.01 31.1 11.0 9.95 9.40 15.8 15.6 69.6 66.8 86.5 91.8 82.9 78.3 87.9 86.7 5:08 6:21 7:06 7:28 7:56 9:02 8:14 7:18 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-11_Form2_FC2G_455S21_SJ1549333.html; Workgroup: WG42273; Design ID: 1980 ] Page 65 of 307 Page 1 of 1 (WG42273 -PFC_FC_LC_PFOA_L18989-11_Form2_FC2G_455S21_SJ1549333.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Project No. Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -T606-DU PA-121204 (Duplicate) Sample Collection: 04-Dec-2012 12:55 FALL 2012 TENNESSEE RIVER SAMPLING WG42273-103 (DUP L18989-11) Matrix: AQUEOUS Sample Size: 0.498 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 13-Dec-2012 Extraction Date: 18-Dec-2012 Instrument ID: LC MS/MS A nalysis Date: 19-Dec-2012 Time: 00:07:06 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC2G_455 S: 22 Injection Volume (uL): 15 Blank Data Filename: FC2G_455 S: 10 Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_455 S: 4 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 5.71 1.00 (L) 1.98 1.00 (L) 2.24 1.00 (L) B 1.88 1.00 (L) 3.77 1.00 (L) U 1.00 (L) U 1.00 (L) U 1.00 (L) U 1.00 (L) U 2.01 (L) U 2.01 (L) 8.62 2.01 (L) U 1.00 (L) 5:08 5:57 6:20 6:42 7:04 8:00 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL; B = analyte found in sample and the associated blank. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42273-103_Form1A_FC2G_455S22_SJ1549334.html; Workgroup: WG42273; Design ID: 1980 ] PPaaggee 66 of 307 Page 1 of 1 (WG42273 -PFC_FC_LC_PFOA_WG42273-103_Form1A_FC2G_455S22_SJ1549334.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Project No. Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -T606-DU PA-121204 (Duplicate) Sample Collection: 04-Dec-2012 12:55 FALL 2012 TENNESSEE RIVER SAMPLING WG42273-103 (DUP L18989-11) Matrix: AQUEOUS Sample Size: 0.498 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 13-Dec-2012 Extraction Date: 18 -D e c-2 0 1 2 Instrument ID: LC MS/MS A nalysis Date: 19-Dec-2012 Time: 00:07:06 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC2G_455 S: 22 Injection Volume (uL): 15 Blank Data Filename: FC2G_455 S: 10 Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_455 S: 4 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 8.67 8.70 34.1 12.2 11.3 11.0 16.1 16.9 72.3 72.5 94.7 102 94.2 91.5 89.2 94.1 5:09 6:20 7:04 7:28 7:56 9:02 8:10 7:15 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42273-103_Form2_FC2G_455S22_SJ1549334.html; Workgroup: WG42273; Design ID: 1980 ] Page 67 of 307 Page 1 of 1 (WG42273 -PFC_FC_LC_PFOA_WG42273-103_Form2_FC2G_455S22_SJ1549334.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 PERFLUORINATED ORGANICS AN ALYSIS REPO RT RELATIVE PERCEN T DIFFERENCE AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Client ID: D A L-S W -T 606-D U P A -121204 Concentration Units: FALL 2012 TENNESSEE RIVER SAMPLING ng/L COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA L18989-11 (A) LAB FLA G 1 CONC. FOUND 6.01 1.59 2.09 2.11 3.62 U U U U 2.14 U 7.45 U WG42273-103 LAB FLAG 1 CONC. FOUND 5.71 1.98 2.24 1.88 3.77 U U U U U U 8.62 U MEAN 5.86 1.79 2.17 1.99 3.70 R E LA T IV E PERCENT D IF F E R E N C E 5.11 21.9 6.48 11.4 4.12 8.03 14.7 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ These pages are part of a larger report that may contain inform ation necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: RPD.xsl; Created: 16-Jan-2013 15:20:57; Application: XMLTransformer-1.13.28; ReportFilename: RPD_FC_LC_PFOA-RPD_WG42273-103_L18989-11_.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - RPD_FC_LC_PFOA-RPD_WG42273-103_L18989-11_.html) Page 68 of 307 www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -T707-DU PA-121204 Sample Collection: 04-Dec-2012 15:10 FALL 2012 TENNESSEE RIVER SAMPLING L18989-12 R Matrix: AQUEOUS Sample Size: 0.458 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 04:27:55 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 33 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 9.21 1.09 (L) 3.68 1.09 (L) 4.65 1.09 (L) 3.93 1.09 (L) 9.73 1.09 (L) U 1.09 (L) U 1.09 (L) U 1.09 (L) U 1.09 (L) 15.0 2.18 (L) U 2.18 (L) 17.3 2.18 (L) U 1.09 (L) 5:12 5:57 6:21 6:44 7:06 6:29 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-12_Form1A_FC3G_015S33_SJ1552196.html; Workgroup: WG42428; Design ID: 1980 ] Page 69 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-12_Form1A_FC3G_015S33_SJ1552196.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. D AL-SW -T707-DU PA-121204 Sample Collection: 04-Dec-2012 15:10 FALL 2012 TENNESSEE RIVER SAMPLING L18989-12 R Matrix: AQUEOUS Sample Size: 0.458 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 04:27:55 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 33 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 9.26 9.68 35.1 13.0 11.3 11.3 17.7 20.0 77.1 80.6 97.5 109 94.2 94.0 98.3 111 5:12 6:21 7:06 7:29 7:59 9:11 8:21 7:20 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-12_Form2_FC3G_015S33_SJ1552196.html; Workgroup: WG42428; Design ID: 1980 ] Page 70 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-12_Form2_FC3G_015S33_SJ1552196.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T808-DU PA-121205 Sample Collection: 05-Dec-2012 10:10 FALL 2012 TENNESSEE RIVER SAMPLING L18989-13 R Matrix: AQUEOUS Sample Size: 0.477 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 04:44:56 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 34 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 8.89 1.05 (L) 2.59 1.05 (L) 3.61 1.05 (L) 3.11 1.05 (L) 7.56 1.05 (L) U 1.05 (L) U 1.05 (L) U 1.05 (L) U 1.05 (L) 11.4 2.10 (L) U 2.10 (L) 12.8 2.10 (L) U 1.05 (L) 5:12 5:57 6:20 6:41 7:03 6:29 8:10 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-13_Form1A_FC3G_015S34_SJ1552197.html; Workgroup: WG42428; Design ID: 1980 ] Page 71 of 307 Page 1 of 1 (WG42428 -PFC_FC_LC_PFOA_L18989-13_Form1A_FC3G_015S34_SJ1552197.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T808-DU PA-121205 Sample Collection: 05-Dec-2012 10:10 FALL 2012 TENNESSEE RIVER SAMPLING L18989-13 R Matrix: AQUEOUS Sample Size: 0.477 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 04:44:56 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 34 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 7.90 8.98 34.0 12.0 10.8 9.52 17.4 17.6 65.9 74.9 94.5 99.8 89.8 79.4 96.6 97.7 5:11 6:18 7:06 7:28 7:59 9:07 8:17 7:17 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-13_Form2_FC3G_015S34_SJ1552197.html; Workgroup: WG42428; Design ID: 1980 ] Page 72 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-13_Form2_FC3G_015S34_SJ1552197.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T909-DU PA-121205 Sample Collection: 05-Dec-2012 12:45 FALL 2012 TENNESSEE RIVER SAMPLING L18989-14 R Matrix: AQUEOUS Sample Size: 0.430 L Sam ple Receipt Date: 14-Dec-2012 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 05:01:49 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 35 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 8.91 1.16 (L) 2.72 1.16 (L) 5.42 1.16 (L) 3.73 1.16 (L) 9.60 1.16 (L) U 1.16 (L) U 1.16 (L) U 1.16 (L) U 1.16 (L) 12.6 2.32 (L) U 2.32 (L) 17.7 2.32 (L) U 1.16 (L) 5:12 5:57 6:22 6:44 7:06 6:29 8:10 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-14_Form1A_FC3G_015S35_SJ1552198.html; Workgroup: WG42428; Design ID: 1980 ] Page 73 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-14_Form1A_FC3G_015S35_SJ1552198.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. DAL-SW -T909-DU PA-121205 Sample Collection: 05-Dec-2012 12:45 FALL 2012 TENNESSEE RIVER SAMPLING L18989-14 R Matrix: AQUEOUS Sample Size: 0.430 L Sam ple Receipt Date: 14 -D e c-2 0 1 2 Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 05:01:49 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 35 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 9.25 9.37 36.2 12.9 10.0 10.1 17.1 17.2 77.1 78.1 101 107 83.6 83.9 95.0 95.8 5:12 6:22 7:06 7:28 7:56 9:02 8:14 7:17 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_L18989-14_Form2_FC3G_015S35_SJ1552198.html; Workgroup: WG42428; Design ID: 1980 ] Page 74 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_L18989-14_Form2_FC3G_015S35_SJ1552198.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Project No. Lab Sam ple I.D.: C LIE N T SA M PLE NO. Lab Blank Sample Collection: N/A N/A WG42273-101 Matrix: AQUEOUS Sample Size: 0.500 L Sam ple Receipt Date: N/A Initial Calibration Date: 13-Dec-2012 Extraction Date: 18-Dec-2012 Instrument ID: LC MS/MS A nalysis Date: 18-Dec-2012 Time: 20:35:06 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC2G_455 S: 10 Injection Volume (uL): 15 Blank Data Filename: FC2G_455 S: 10 Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_455 S: 4 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 1.00 (L) U 1.00 (L) U 1.00 (L) B 1.48 1.00 (L) U 1.00 (L) U 1.00 (L) U 1.00 (L) U 1.00 (L) U 1.00 (L) U 2.00 (L) U 2.00 (L) U 2.00 (L) U 1.00 (L) 6:45 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL; B = analyte found in the blank. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42273-101_Form1A_FC2G_455S10_SJ1549320.html; Workgroup: WG42273; Design ID: 1980 ] PPaaggee 75 of 307 Page 1 of 1 (WG42273 -PFC_FC_LC_PFOA_WG42273-101_Form1A_FC2G_455S10_SJ1549320.html) www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Project No. Lab Sam ple I.D.: CLIENT SAMPLE NO. LSaabmBplalen kC o lle c t i o n : N/A N/A WG42273-101 Matrix: AQUEOUS Sample Size: 0.500 L Sam ple Receipt Date: N/A Initial Calibration Date: 13-Dec-2012 Extraction Date: 18 -D e c-2 0 1 2 Instrument ID: LC MS/MS A nalysis Date: 18-Dec-2012 Time: 20:35:06 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC2G_455 S: 10 Injection Volume (uL): 15 Blank Data Filename: FC2G_455 S: 10 Dilution Factor: N/A Cal. Ver. Data Filename: FC2G 455 S: 4 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 10.1 11.3 34.0 10.5 9.72 12.3 15.5 15.9 84.4 94.4 94.3 87.4 81.0 103 86.1 88.4 5:11 6:21 7:06 7:28 8:00 9:02 8:14 7:18 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42273-101_Form2_FC2G_455S10_SJ1549320.html; Workgroup: WG42273; Design ID: 1980 ] Page 76 of 307 Page 1 of 1 (WG42273 - PFC_FC_LC_PFOA_WG42273-101_Form2_FC2G_455S10_SJ1549320.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Project No. Lab Sam ple I.D.: C LIE N T SA M PLE NO. Lab Blank Sample Collection: N/A N/A WG42428-101 Matrix: AQUEOUS Sample Size: 0.500 L Sam ple Receipt Date: N/A Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 01:21:16 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 22 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_015 S: 16 Concentration Units: ng/L This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U U U U U U U U U U U U U 1.00 (L) 1.00 (L) 1.00 (L) 1.00 (L) 1.00 (L) 1.00 (L) 1.00 (L) 1.00 (L) 1.00 (L) 2.00 (L) 2.00 (L) 2.00 (L) 1.00 (L) (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42428-101_Form1A_FC3G_015S22_SJ1552184.html; Workgroup: WG42428; Design ID: 1980 ] PPaaggee 77 of 307 Page 1 of 1 (WG42428 -PFC_FC_LC_PFOA_WG42428-101_Form1A_FC3G_015S22_SJ1552184.html) www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Project No. Lab Sam ple I.D.: CLIENT SAMPLE NO. LSaabmBplalen kC o lle c t i o n : N/A N/A WG42428-101 Matrix: AQUEOUS Sample Size: 0.500 L Sam ple Receipt Date: N/A Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 01:21:16 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_015 S: 22 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G 015 S: 16 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 12.7 13.3 31.5 13.1 10.9 12.4 19.5 20.9 106 110 87.5 109 90.5 103 108 116 5:12 6:21 7:06 7:29 7:59 9:11 8:21 7:20 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42428-101_Form2_FC3G_015S22_SJ1552184.html; Workgroup: WG42428; Design ID: 1980 ] Page 78 of 307 Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_WG42428-101_Form2_FC3G_015S22_SJ1552184.html) www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 8A PERFLU O RIN A TED O RG AN ICS ONGOING PRECISIO N AND R EC O V ER Y (OPR) AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Lab Sam ple I.D.: W G 42273-102 Matrix: AQUEOUS Initial Calibration Date: 13-Dec-2012 Extraction Date: 18-Dec-2012 Instrument ID: LC MS/MS A nalysis Date: 18-Dec-2012 Time: 19:42:48 Colum n ID: C18MS Extract Volume (uL): 4000 O PR Data Filename: FC2G_455 S: 7 Injection Volume (uL): 15 Blank Data Filename: FC2G_455 S: 10 Dilution Factor: N/A Cal. Ver. Data Filename: FC2G 455 S: 4 A L L C O N C EN T R A TIO N S R E P O R T E D ON TH IS FORM A R E C O N C EN T R A TIO N S IN E X T R A C T , B A S E D O N A 1 m L E X T R A C T VOLUM E. COMPOUND LAB FLA G 1 SPIKE CONC. (ng/mL) CONC. FOUND (ng/mL) % RECOVERY R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 40.0 40.0 40.0 20.0 19.0 17.7 20.0 19.0 19.5 19.8 22.8 20.9 20.2 43.3 40.1 38.8 22.5 95.0 88.7 100 95.2 97.4 99.0 114 105 101 108 100 97.0 112 5:06 5:57 6:20 6:42 7:06 7:28 7:56 8:30 9:04 6:27 7:18 8:14 9:54 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ These pages are part o f a larger report that may contain inform ation necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: FC-Form8A.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42273-102_Form8A_SJ1549315.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFC_FC_LC_PFOA_WG42273-102_Form8A_SJ1549315.html) Page 79 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 8B PERFLUORINATED ORGANICS ONGOING PRECISION AND RECOVERY (OPR) AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Lab Sam ple I.D.: W G 42273-102 Matrix: AQUEOUS Initial Calibration Date: 13-Dec-2012 Extraction Date: 18-Dec-2012 Instrument ID: LC MS/MS A nalysis Date: 18-Dec-2012 Time: 19:42:48 Colum n ID: C18MS Extract Volume (uL): 4000 O PR Data Filename: FC2G_455 S: 7 Injection Volume (uL): 15 Blank Data Filename: FC2G_455 S: 10 Dilution Factor: N/A Cal. Ver. Data Filename: FC2G_455 S: 4 A L L C O N C EN T R A TIO N S R E P O R T E D ON TH IS FORM A R E C O N C EN T R A TIO N S IN E X T R A C T , B A S E D O N A 1 m L E X T R A C T VOLUM E LABELED COMPOUND LAB FLA G 1 SPIKE CONC. (ng/mL) CONC. FOUND (ng/mL) % RECOVERY RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 10.6 11.4 33.3 11.4 9.64 11.7 16.6 17.7 88.0 94.7 92.5 94.6 80.3 97.9 92.3 98.5 5:06 6:20 7:06 7:28 7:56 9:04 8:14 7:18 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ These pages are part o f a larger report that may contain inform ation necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: FC-Form8B.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42273-102_Form8B_SJ1549315.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFC_FC_LC_PFOA_WG42273-102_Form8B_SJ1549315.html) Page 80 of 307 www.axysanalytical.com A X Y S METHOD MLA-060 Rev 10 Form 8A PERFLU O RIN A TED O RG AN ICS ONGOING PRECISIO N AND R EC O V ER Y (OPR) AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Lab Sam ple I.D.: W G 42428-102 Matrix: AQUEOUS Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 00:30:21 Colum n ID: C18MS Extract Volume (uL): 4000 O PR Data Filename: FC3G_015 S: 19 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G 015 S: 16 A L L C O N C EN T R A TIO N S R E P O R T E D ON TH IS FORM A R E C O N C EN T R A TIO N S IN E X T R A C T , B A S E D O N A 1 m L E X T R A C T VOLUM E. COMPOUND LAB FLA G 1 SPIKE CONC. (ng/mL) CONC. FOUND (ng/mL) % RECOVERY R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 40.0 40.0 40.0 20.0 19.2 19.2 22.3 21.1 23.7 21.8 22.3 23.1 22.5 44.4 43.1 46.8 23.7 96.2 96.0 111 106 119 109 112 115 113 111 108 117 119 5:11 5:56 6:20 6:44 7:06 7:29 7:59 8:35 9:11 6:29 7:20 8:21 9:59 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ These pages are part o f a larger report that may contain inform ation necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: FC-Form8A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42428-102_Form8A_SJ1552180.html; Workgroup: WG42428; Design ID: 1980 ] Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_WG42428-102_Form8A_SJ1552180.html) Page 81 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 8B PERFLUORINATED ORGANICS ONGOING PRECISION AND RECOVERY (OPR) AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Lab Sam ple I.D.: W G 42428-102 Matrix: AQUEOUS Initial Calibration Date: 10-Jan-2013 Extraction Date: 14-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 Time: 00:30:21 Colum n ID: C18MS Extract Volume (uL): 4000 O PR Data Filename: FC3G_015 S: 19 Injection Volume (uL): 15 Blank Data Filename: FC3G_015 S: 22 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G 015 S: 16 A L L C O N C EN T R A TIO N S R E P O R T E D ON TH IS FORM A R E C O N C EN T R A TIO N S IN E X T R A C T , B A S E D O N A 1 m L E X T R A C T VOLUM E LABELED COMPOUND LAB FLA G 1 SPIKE CONC. (ng/mL) CONC. FOUND (ng/mL) % RECOVERY RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 12.3 11.8 30.1 11.6 10.1 11.7 16.6 19.1 102 98.7 83.6 96.3 84.0 97.3 92.2 106 5:12 6:20 7:06 7:29 7:59 9:11 8:21 7:20 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ These pages are part o f a larger report that may contain inform ation necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: FC-Form8B.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFC_FC_LC_PFOA_WG42428-102_Form8B_SJ1552180.html; Workgroup: WG42428; Design ID: 1980 ] Page 1 of 1 (WG42428 - PFC_FC_LC_PFOA_WG42428-102_Form8B_SJ1552180.html) Page 82 of 307 www.axysanalytical.com ____________ PfC Sample List Report WlassLynx 4.1 SCN627 Sample List: G:\MassIynx\lnst_G\_Master.PRO\SampleDB\FC2G_445.SPL Last Modified: Thursday, December 13, 2012 11:30:57 Pacific Standard Time Printed: Thursday, December 13, 2012 14:52:46 Pacific Standard Time Page 1 of 1 Page Position (1,1) File Name Sample ID File Text Bottle Signln SignOut InjVoL 1 FC2G 445S001 MeOH,, 2 FC2G 445S002 MeOH,, 3 FC2G 445S003 FC-LIN-42221W500-A,, 4 FC2G 445S004 FC-LIN-42221W500-A,, 5 FC2G 445S005 FC-LIN-42221W500-B,, 6 FC2G 445S006 FC-LIN-42221W500-C,, 7 FC2G 445S007 FC-LIN-42221W500-D,, 8 FC2G 445S008 FC-LIN-42221W500-E,, 9 FC2G 445S009 FC-LIN-42221 W500-F,, 10 FC2G 445S010 FC-LIN-42221 W500-G,, 11 FC2G 445S011 FC-LIN-42221W500-H,, 12 FC2G 445S012 MeOH,, 13 FC2G 445S013 MeOH,, 14 FC2G 445S014 FC-42221W500-SAR2,, 15 FC2G 445S015 FC-42221W500-SAR,, 16 FC2G 445S016 FC-LIN-42221W500-D,, 17 FC2G 445S017 FC-LIN-42221W500-D,, 18 FC2G 445S018 WG42214-103,,SAR 19 FC2G 445S019 WG42214-102,,SPM 20 FC2G 445S020 MeOH,, 21 FC2G 445S021 MeOH,, 22 FC2G 445S022 WG42214-101,,BLK 23 FC2G 445S023 L18938-1 ,, 24 FC2G 445S024 L18938-2,, 25 FC2G 445S025 L18938-3,, 26 FC2G 445S026 L18955-1,, 27 FC2G 445S027 L18955-2,, 28 FC2G 445S028 L18955-3,, 29 FC2G 445S029 L18955-4,, 30 FC2G 445S030 L18955-5,, 31 FC2G 445S031 L18955-6,, 32 FC2G 445S032 L18955-7,, 33 FC2G 445S033 L18970-1,, 34 FC2G 445S034 L18970-2,, 35 FC2G 445S035 L18970-3,, 36 FC2G 445S036 L18970-4,, 37 FC2G~445S037 FC-LIN-42221W500-D,, 38 FC2G 445S038 FC-LIN-42221W500-D,, 39 FC2G 445S039 MeOH,, 40 FC2G 445S040 MeOH,, 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL . 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL ' 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,,15uL 1,,15uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1:8,A 1:8,A 1:1,A 1:1,A 1:1,B 1:1,C 1:1,D 1:1,E 1:1,F 1:2,A 1:2,B 1:8,A 1:8,A 1:2,C 1:2,D 1:1,D 1:1,D 1:2,E 1:2,F 1:8,A 1:8,A 1:6,E 1:6,F 1:7,A 1:7,B 1:7,C 1:7,D 1:7,E 1:7,F 1:8,B 1:8, C 1:8,D 1:8,E 1:8,F 1:4,E 1:4,F 1:1,D 1:1,D 1:8,A 1:8,A --- Hr -1 - -- -- -- -- - - - - - -- -- - ' - - - -- -1 -- 4 -- 4 -- 4 -4 -- 4 - - -] - " - -- - -" - -~ -r -- i /Ji rqJ - - -- -r -f ---\Ti!. -1 -4_ --\ \ 1 - -- 4- 4 -i -T - -, -T- - -- -77 - -- i f/ -V 15.0 15.0 15.0 15.0 - 15.0 15.0 15.0 \ A \ 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 11^5.0 15.0 15.0 15.0 15.0 15.0 1155.-0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15` 15- 15.0 Page 83 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 3A PERFLUORINATED ORGANICS INITIAL CALIBRATION PERCENT RECOVERIES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 13-Dec-2012 CS0 Data Filename: CS1 Data Filename: FC2G_445 S: 4 FC2G_445 S: 5 Instrument ID: LC MS/MS CS2 Data Filename: FC2G_445 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC2G_445 S: 7 C S4 Data Filename: FC2G_445 S: 8 CS5 Data Filename: FC2G_445 S: 9 C S6 Data Filename: FC2G_445 S: 10 CS7 Data Filename: FC2G_445 S: 11 CS8 Data Filename: N/A COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 CS0 113 122 111 120 113 114 85.2 106 92.6 103 102 111 123 CS1 110 102 103 96.3 94.3 90.9 110 104 107 104 117 104 107 CS2 90.2 87.5 90.4 90.0 94.1 96.6 97.9 95.4 96.2 87.8 84.2 88.6 79.5 P ER C E N T R EC O V ER Y (%) CS3 CS4 CS5 88.3 90.1 94.8 90.4 95.8 95.3 104 91.6 102 96.2 89.7 92.5 91.3 99.5 95.3 98.3 99.9 104 103 101 97.7 100 111 105 102 96.0 96.1 102 104 106 99.6 101 107 111 104 103 105 103 104 CS6 103 101 98.5 97.6 99.4 99.1 94.9 94.4 96.8 95.6 96.6 98.1 99.6 CS7 99.6 99.8 100 100 100 100 101 101 102 101 100 100 CS8 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3A.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_13-Dec-2012_FC2G__Form3A_GS48529.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFOA_FC_LC_13-Dec-2012_FC2G__Form3A_GS48529.html) Page 84 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 3B PERFLUORINATED ORGANICS INITIAL CALIBRATION PERCENT RECOVERIES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 13-Dec-2012 CS0 Data Filename: CS1 Data Filename: FC2G_445 S: 4 FC2G_445 S: 5 Instrument ID: LC MS/MS CS2 Data Filename: FC2G_445 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC2G_445 S: 7 C S4 Data Filename: FC2G_445 S: 8 CS5 Data Filename: FC2G_445 S: 9 C S6 Data Filename: FC2G_445 S: 10 CS7 Data Filename: FC2G_445 S: 11 CS8 Data Filename: N/A LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS LAB FLA G 1 CS0 101 107 94.6 98.9 94.3 84.4 98.5 105 CS1 98.8 109 98.1 116 97.9 86.4 112 104 P ER C E N T R EC O V ER IES (%) CS2 CS3 CS4 CS5 CS6 98.3 100 103 104 100 102 99.4 104 92.8 95.2 94.6 95.2 101 104 102 98.9 99.3 102 101 97.0 87.9 89.0 102 92.3 111 88.9 88.5 104 111 137 103 99.3 110 96.9 101 110 110 105 97.1 94.8 CS7 93.8 90.9 110 86.3 125 79.6 73.9 CS8 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3B.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_13-Dec-2012_FC2G__Form3B_GS48529.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFOA_FC_LC_13-Dec-2012_FC2G__Form3B_GS48529.html) Page 85 of 307 www.axysanalytical.com A X Y S M ETHOD MLA-060 Rev 10 Form 3C PERFLU O RIN ATED O RGAN ICS INITIAL CALIBRATION RETENTION TIMES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 13-Dec-2012 C S0 Data Filename: CS1 Data Filename: FC2G_445 S: 4 FC2G_445 S: 5 Instrument ID: LC MS/MS C S2 Data Filename: FC2G_445 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC2G_445 S: 7 C S4 Data Filename: FC2G_445 S: 8 C S5 Data Filename: FC2G_445 S: 9 C S6 Data Filename: FC2G_445 S: 10 C S7 Data Filename: FC2G_445 S: 11 C S8 Data Filename: N/A COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 CS0 5:08 5:58 6:23 6:45 7:06 7:29 8:00 8:30 9:06 6:29 7:18 8:14 9:54 CS1 5:10 5:58 6:21 6:45 7:06 7:28 7:56 8:30 9:06 6:28 7:18 8:14 9:52 CS2 5:09 5:59 6:23 6:45 7:06 7:29 8:00 8:32 9:07 6:29 7:18 8:17 9:54 RETENTION TIMES CS3 CS4 CS5 5:11 5:59 6:23 6:45 7:06 7:29 8:00 8:32 9:06 6:29 7:18 8:14 9:54 5:06 5:58 6:21 6:45 7:06 7:29 8:00 8:32 9:07 6:28 7:18 8:14 9:56 5:09 5:59 6:21 6:45 7:06 7:29 8:00 8:30 9:06 6:29 7:18 8:14 9:54 CS6 5:06 5:57 6:19 6:42 7:04 7:28 7:56 8:30 9:06 6:27 7:18 8:14 9:54 CS7 5:12 5:59 6:21 6:45 7:06 7:29 8:00 8:33 9:07 6:28 7:18 8:14 CS8 MEAN RT 5:09 5:58 6:22 6:45 7:06 7:29 7:59 8:31 9:06 6:28 7:18 8:14 9:54 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3C.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_13-Dec-2012_FC2G__Form3C_GS48529.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFOA_FC_LC_13-Dec-2012_FC2G__Form3C_GS48529.html) Page 86 of 307 www.axysanalytical.com A X Y S M ETHOD MLA-060 Rev 10 Form 3D PERFLU O RIN ATED O RGAN ICS INITIAL CALIBRATION RETENTION TIMES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 13-Dec-2012 C S0 Data Filename: CS1 Data Filename: FC2G_445 S: 4 FC2G_445 S: 5 Instrument ID: LC MS/MS C S2 Data Filename: FC2G_445 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC2G_445 S: 7 C S4 Data Filename: FC2G_445 S: 8 C S5 Data Filename: FC2G_445 S: 9 C S6 Data Filename: FC2G_445 S: 10 C S7 Data Filename: FC2G_445 S: 11 C S8 Data Filename: N/A LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS LAB FLAG1 RETENTION TIMES CS0 CS1 CS2 CS3 CS4 CS5 CS6 CS7 5:08 6:23 7:06 7:28 8:00 9:06 8:14 7:18 5:09 6:21 7:06 7:28 7:56 9:06 8:14 7:18 5:09 6:21 7:06 7:29 8:00 9:07 8:17 7:18 5:11 6:21 7:06 7:29 8:00 9:06 8:14 7:18 5:08 6:21 7:06 7:28 8:00 9:07 8:14 7:18 5:09 6:21 7:06 7:29 8:00 9:04 8:14 7:18 5:04 6:19 7:04 7:28 7:56 9:06 8:14 7:18 5:10 6:21 7:06 7:29 8:00 8:17 7:18 CS8 MEAN RT 5:09 6:21 7:06 7:29 7:59 9:06 8:15 7:18 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3D.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_13-Dec-2012_FC2G__Form3D_GS48529.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFOA_FC_LC_13-Dec-2012_FC2G__Form3D_GS48529.html) Page 87 of 307 www.axysanalytical.com 473/______ F?0- Aj^ Sample List Report MassLynx 4.1 SCN627 Sample List: G:\Masslynx\lnst_G\_Master.PRO\SampleDB\FC2G_455.SPL Last Modified: Tuesday, December 18, 2012 16:01:48 Pacific Standard Time Printed: Tuesday, December 18, 2012 16:07:45 Pacific Standard Time Page 1 of 1 Page Position (1,1) lift; rc M i-W S - A l File Name Sample ID 1 FC2G 455S001 MeOH,, 2 FC2G 455S002 MeOH,, 3 FC2G 455S003 FC-L1N-42221W500-A,, 4 FC2G 455S004 FC-LIN-42221W500-D,, 5 FC2G 455S005 FC-LIN-42221W500-D,, 6 FC2G 455S006 WG42273-104,,SAR 7 FC2G 455S007 WG42273-1Q2,,SPM 8 FC2G 455S008 MeOH,, 9 FC2G 455S009 MeOH,, 10 FC2G 455S010 WG42273-101,,BLK 11 FC2G 455S011 L18989-1,, 12 FC2G 455S012 L18989-2,, 13 FC2G 455S013 L18989-3,, 14 FC2G 455S014 L18989-4,, 15 FC2G 455S015 L18989-5,, 16 FC2G 455S016 L18989-6,, 17 FC2G 455S017 L18989-7,, 18 FC2G 455S018 L18989-8,, 19 FC2G 455S019 L18989-9,, 20 FC2G 455S020 L18989-10,, 21 FC2G 455S021 L18989-11,, 22 FC2G 455S022 WG42273-103,,DUP 23 FC2G 455S023 L18989-12,, 24 FC2G 455S024 L18989-13,, 25 FC2G 455SQ25 L18989-14,, 26 FC2G 455S026 L18992-1,, 27 FC2G 455S027 L18992-2,, 28 FC2G 455S028 FC-LIN-42221W500-D,, 29 FC2G 455S029 FC-LIN-42221W500-D,, 30 FC2G 455S030 MeOH,, 31 FC2G 455S031 MeOH,, File Text Bottle s ignln 1,,15uL ' 1:8,A 1,,15uL 1:8,A 1,,15uL 1:1,A 1,,15uL 1:1,D 1,,15uL 1:1,D 1,WG42273,15/4000uL 3:2,B 1,WG42273,15/4000uL 3:2,0 LM t i-_ ' -- 1,,15uL . 1:8,A 1,,15uL 1:8,A - 1,WG42273,15/4000uL 3:2,D - - 1.WG42273,15/4000uL 3:2,E 1,WG42273,15/4000uL 3:2,F 1,WG42273,15/4000uL 3:3,A 1,WG42273,15/4000uL 3:3,B 1,WG42273,15/4000uL 3:3,0 1,WG42273,15/4000uL 3:3,D 1,WG42273,15/4000uL 3:3,E 1,WG42273,15/4000UL 3:3,F 1,WG42273,15/4000uL 3:4,A 1,WG42273,15/4000uL 3:4,B 1,WG42273,15/4000uL 3:4,0 1,WG42273,15/4000uL 3:4,D 1,WG42273,15/4000UL 3:4,E 1,WG42273,15/4000uL 3:4,F - -- -....-- ----- ---, - 1,WG42273,15/4000uL 3:5,A T 1,WG42273,15/4000uL 3:5,B 1,WG42273,15/4000uL 3:5,0 1,,15.uL 1:1,D - 1,,15uL 1,,15uL 1,,15uL 1:1,D 1:8,A --) 1:8,A w Signut Inj VoL - 15.0 - 15.0 - 15.0 -1 - - 15.0 - - 15.0 - 15.0 15.0 - 15.0 - 15.0 - - 15.0 - - 15.0 - 15.0 - 15.0 \ 15.0 - - 15.0 15.0 - 15.0 - 15.0 15.0 ; - 15.0 -- 15.0 -- 15.0 - 15.0 - 15.0 15.0 -, 15.0 - 15.0 --. 15.0 - 15.0_ y 15.0 ^ 15.0 B Page 88 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 4A PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 13 -D e c-2 0 1 2 V E R Data Filename: FC2G_455 S: 4 Instrument ID: LC MS/MS A nalysis Date: 18 -D e c -2 0 1 2 L C Colum n ID: C18MS A nalysis Time: 18:49:50 COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 RETEN TION TIME 5:11 5:58 6:21 6:42 7:06 7:28 7:56 8:30 9:04 6:27 7:15 8:14 9:54 EXPECTED CONC. (ng) 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 40.0 40.0 40.0 20.0 CONC. FOUND (ng) 17.4 16.6 19.0 18.1 18.8 19.8 20.9 17.6 20.5 40.7 39.5 39.1 18.1 R E C O V E R Y (%) 87.0 82.9 95.0 90.3 93.8 99.0 105 88.2 103 102 98.6 97.7 90.3 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; Report Filename: PFOA_FC_LC_FC2G_455S4__Form4A_SJ1549312.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFOA_FC_LC_FC2G_455S4__Form4A_SJ1549312.html) Page 89 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 4B PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 13 -D e c-2 0 1 2 V E R Data Filename: FC2G_455 S: 4 Instrument ID: LC MS/MS A nalysis Date: 18 -D e c -2 0 1 2 L C Colum n ID: C18MS A nalysis Time: 18:49:50 LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS LAB FLAG 1 RETEN TION TIME 5:11 6:20 7:06 7:28 7:56 9:04 8:14 7:18 EXPECTED CONC. (ng) 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 CONC. FOUND (ng) 12.4 12.9 34.5 12.8 11.9 10.7 18.4 20.0 R E C O V E R Y (%) 104 108 96.0 107 99.3 88.9 102 111 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_FC2G_455S4__Form4B_SJ1549312.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFOA_FC_LC_FC2G_455S4__Form4B_SJ1549312.html) Page 90 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 4A PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 13 -D e c-2 0 1 2 V E R Data Filename: FC2G_455 S: 28 Instrument ID: LC MS/MS A nalysis Date: 19 -D e c -2 0 1 2 L C Colum n ID: C18MS A nalysis Time: 01:53:10 COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 RETEN TION TIME 5:10 5:59 6:23 6:45 7:06 7:29 7:56 8:28 9:02 6:28 7:18 8:14 9:54 EXPECTED CONC. (ng) 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 40.0 40.0 40.0 20.0 CONC. FOUND (ng) 18.0 17.4 19.0 19.4 18.5 19.3 22.1 19.3 20.4 38.4 35.3 38.3 17.7 R E C O V E R Y (%) 90.1 86.8 95.0 97.0 92.5 96.7 110 96.7 102 96.0 88.4 95.8 88.6 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_FC2G_455S28_Form4A_SJ1549340.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFOA_FC_LC_FC2G_455S28__Form4A_SJ1549340.html) Page 91 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 4B PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 13 -D e c-2 0 1 2 V E R Data Filename: FC2G_455 S: 28 Instrument ID: LC MS/MS A nalysis Date: 19 -D e c -2 0 1 2 L C Colum n ID: C18MS A nalysis Time: 01:53:10 LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS LAB FLAG 1 RETEN TION TIME 5:08 6:21 7:06 7:28 7:56 9:02 8:14 7:18 EXPECTED CONC. (ng) 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 CONC. FOUND (ng) 11.9 12.0 37.0 12.6 10.6 10.3 18.3 20.7 R E C O V E R Y (%) 99.6 100 103 105 88.1 85.8 101 115 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 16-Jan-2013 15:20:40; Application: XMLTransformer-1.13.28; Report Filename: PFOA_FC_LC_FC2G_455S28_Form4B_SJ1549340.html; Workgroup: WG42273; Design ID: 1980 ] Page 1 of 1 (WG42273 - PFOA_FC_LC_FC2G_455S28__Form4B_SJ1549340.html) Page 92 of 307 www.axysanalytical.com __________________________________________________________ 4 - 7 * 1 Sample List Report PFC - M ____________________________________________________ ;_____________________________________________________________ MassLynx 4.1 SCN627 Sample List: G:\Masslynx\lnst_G\_Master.PRO\SampleDB\FC3G_015.SPL Last Modified: Monday, January 14, 2013 15:51:45 Pacific Standard Time Printed: Monday, January 14, 2013 15:58:34 Pacific Standard Time Page 1of 1 Page Position (1,1) File Name S a m p le ID File Text B ottle S ignln S ig n O u t Inj VoL Li<A 1 FC3G 015S001 MeOH,, 1,,15uL 1:8,A 2 FC3G 015S002 MeOH,, 1,,15uL 1:8,A - 3 FC3G 015S003 FC-L1N-42420W500-A,, 1,,15uL 2:1,A f- 4 FC3G 015S004 FC-LlN-42420W500-A,, 1,,15uL 2:1,A 5 FC3G 015S005 FC-LIN-42420W500-B,, 1,,15uL 2:1 ,B 6 FC3G '015S006 FC-LIN-42420W500-C,, 1,,15uL 2:1,C - - 7 FC3G "015S007 FC-LI N-42420W500-D,, 1,,15uL 2:1,D .- - 8 FC3G 015S008 FC-LIN-42420W500-E,, 1,,15uL 2:1,E - - 9 FC3G 015S009 FC-LIN-42420W500-F,, 1,,15uL 2:1,F - - 10 FC3G 015S010 FC-LI N-42420W500-G,, 1,,15uL 2:2,A " 11 FC3G 015S011 FC-LI N-42420W500-H,, 1,,15uL 2:2,B - - - 12 FC3G 015S012 MeOH,, 1,,15uL 1:8,A - - 13 FC3G 015S013 MeOH,, 1,,15uL 1:8,A 14 FC3G* 015S014 FC-42420W500-SAR2,, 1,,15uL 2:2,C - 15 FC3G~ 015S015 FC-42420W500-SAR,, 1,,15uL 2:2,D - 16 FC3G 015S016 FC-UN-42420W500-D,, 1,,15uL 2:1,D - - 17 FC3G 015S017 FC-LIN-42420W500-D,, 1,,15uL 2:1,D* - 18 FC3G 015S018 WG42428-103,,SAR 1,WG42428,15/4000uL 2:2,E - - - 19 FC3G 015S019 WG42428-102,,SPM 20 FC3G "015S020 MeOH,, 1,WG42428,15/4000uL 2:2,F - **- 1,,15uL 1:8,A - - 21 FC3G 015S021 MeOH,, 1,,15uL 1:8,A - - 22 FC3G 015S022 WG42428-101 ,,BLK 1,WG42428,15/4000uL 2:3,A ' - - 23 FC3G 015S023 L18989-1 ,R, 1,WG42428,15/4000uL 2:3,B- - - 24 FC3G 015S024 L18989-2,R, 1,WG42428,15/4000uL 2:3,C - - 25 FC3G 015S025 L18989-3,R, 1,WG42428,15/4000uL 2:3,D - - 26 FC3G 015S026 L18989-4,R, 1,WG42428,15/4000uL 2:3,E - - 27 FC3G 015S027 L18989-5,R, 1,WG42428,15/4000uL 2:3,F - - 28 FC3G 015S028 L18989-6,R, 1,WG42428,15/4000uL 2:4,A- 29 FC3G 015S029 L18989-7,R, 1,WG42428,15/4000uL 2:4,B - _ , "" -- -- 4 4 4 4 T4 4 4 4 4 __ "I -- -- -- -- -- -- -- -- -- -- -- -- 15.0 15.0 15.0 is.o n 15.0 15.0 15.0 ) 15.0 15,0 15.0 15.0 - 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 30 FC3G 015S030 L18989-8,R, 1,WG42428,15/4000uL 2:4,C - 31 FC3G 015S031 L18989-9,R, 1,WG42428,15/4000uL 2:4,D -- 32 FC3G 015S032 L18989-10,R, 1,WG42428,15/4000uL 2:4,E -- 33 FC3G 015S033 L18989-12.R, 1,WG42428,15/4000uL 2:4,F- -- 34 FC3G 015S034 L18989-13,R, 35 FC3G 015S035 L18989-14,R, 1,WG42428,15/4000uL 2:5,A 1,WG42428,15/4000uL 2:5,B . 36 FC3G 015S036 FC-LI N-42420W500-D,, 1,,15uL 37 FC3G 015S037 FC-LI N-42420W500-D,, 1,,15uL 2:1,D 2:1,D -~-, 38 FC3G 015S038 MeOH,, 39 FC3G '015S039 MeOH,, 1,,15uL 1,,15uL 1:8,A 1:8,A yt -- --- -- -- -- -- 15,0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 Page 93 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 3A PERFLU O RIN ATED ORGAN ICS INITIAL CALIBRATION PER C EN T RECO V ER IES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 10-Jan-2013 CS0 Data Filename: CS1 Data Filename: FC3G_015 S: 4 FC3G_015 S: 5 Instrument ID: LC MS/MS CS2 Data Filename: FC3G_015 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC3G_015 S: 7 C S4 Data Filename: FC3G_015 S: 8 CS5 Data Filename: FC3G_015 S: 9 C S6 Data Filename: FC3G_015 S: 10 CS7 Data Filename: FC3G_015 S: 11 CS8 Data Filename: N/A COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 CS0 92.2 109 93.8 91.8 81.1 87.2 86.5 87.9 73.2 82.5 90.4 101 100 CS1 115 109 112 106 113 107 99.7 99.8 110 105 112 104 94.3 CS2 101 91.1 95.9 103 104 103 104 104 107 98.5 89.9 102 110 P ER C E N T R EC O V ER Y (%) CS3 CS4 CS5 94.9 93.7 99.3 101 99.1 105 103 102 105 110 106 95.8 108 97.7 95.8 98.8 97.2 103 98.9 107 105 105 106 101 95.9 85.4 95.4 99.1 99.1 102 101 96.8 103 107 105 99.6 103 101 99.6 CS6 104 102 101 99.7 98.4 102 94.3 93.7 94.2 97.9 97.8 101 103 CS7 99.5 99.7 99.8 100 100 99.8 102 101 103 100 100 99.9 99.7 CS8 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form3A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_10-Jan-2013_FC3G__Form3A_GS48596.html; Workgroup: WG42428; Design ID: 1980 ] Page 1 of 1 (WG42428 -PFOA_FC_LC_10-Jan-2013_FC3G__Form3A_GS48596.html) Page 94 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 3B PERFLU O RIN ATED ORGAN ICS INITIAL CALIBRATION PER C EN T RECO V ER IES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 10-Jan-2013 CS0 Data Filename: CS1 Data Filename: FC3G_015 S: 4 FC3G_015 S: 5 Instrument ID: LC MS/MS CS2 Data Filename: FC3G_015 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC3G_015 S: 7 C S4 Data Filename: FC3G_015 S: 8 CS5 Data Filename: FC3G_015 S: 9 C S6 Data Filename: FC3G_015 S: 10 CS7 Data Filename: FC3G_015 S: 11 CS8 Data Filename: N/A LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS LAB FLA G 1 CS0 109 107 95.9 99.6 78.9 90.5 103 109 CS1 108 111 95.3 106 98.7 85.3 113 108 P ER C E N T R EC O V ER IES (%) CS2 CS3 CS4 CS5 CS6 103 106 99.7 102 92.7 97.6 105 113 102 101 99.8 102 95.9 99.0 106 106 99.2 97.4 99.0 101 94.6 94.7 109 99.4 98.6 96.8 98.2 106 95.2 111 98.6 100 87.2 86.1 106 89.1 107 122 85.5 87.6 CS7 92.6 94.4 106 94.0 137 79.5 77.0 CS8 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form3B.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_10-Jan-2013_FC3G__Form3B_GS48596.html; Workgroup: WG42428; Design ID: 1980 ] Page 1 of 1 (WG42428 - PFOA_FC_LC_10-Jan-2013_FC3G__Form3B_GS48596.html) Page 95 of 307 www.axysanalytical.com A X Y S M ETHOD MLA-060 Rev 10 Form 3C PERFLU O RIN ATED O RGAN ICS INITIAL CALIBRATION RETENTION TIMES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 10-Jan-2013 C S0 Data Filename: CS1 Data Filename: FC3G_015 S: 4 FC3G_015 S: 5 Instrument ID: LC MS/MS C S2 Data Filename: FC3G_015 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC3G_015 S: 7 C S4 Data Filename: FC3G_015 S: 8 C S5 Data Filename: FC3G_015 S: 9 C S6 Data Filename: FC3G_015 S: 10 C S7 Data Filename: FC3G_015 S: 11 C S8 Data Filename: N/A COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 CS0 5:12 5:57 6:22 6:44 7:09 7:33 8:03 8:37 9:15 6:31 7:20 8:24 9:59 CS1 5:11 5:57 6:21 6:44 7:06 7:33 8:03 8:37 9:15 6:29 7:20 8:24 9:59 CS2 5:11 5:58 6:22 6:44 7:09 7:33 8:03 8:37 9:15 6:30 7:20 8:24 9:59 RETENTION TIMES CS3 CS4 CS5 5:14 5:57 6:21 6:44 7:09 7:33 8:03 8:37 9:13 6:30 7:20 8:24 9:59 5:15 5:58 6:22 6:44 7:09 7:33 8:03 8:37 9:13 6:30 7:20 8:21 9:59 5:14 5:58 6:22 6:44 7:09 7:33 8:03 8:39 9:15 6:30 7:20 8:24 9:59 CS6 5:14 5:57 6:20 6:44 7:06 7:29 8:03 8:37 9:13 6:29 7:20 8:21 9:59 CS7 5:15 5:58 6:21 6:44 7:06 7:29 8:03 8:35 9:11 6:30 7:20 8:21 9:59 CS8 MEAN RT 5:13 5:58 6:21 6:44 7:08 7:32 8:03 8:37 9:14 6:30 7:20 8:23 9:59 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form3C.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_10-Jan-2013_FC3G__Form3C_GS48596.html; Workgroup: WG42428; Design ID: 1980 ] Page 1 of 1 (WG42428-PFOA_FC_LC_10-Jan-2013_FC3G__Form3C_GS48596.html) Page 96 of 307 www.axysanalytical.com A X Y S M ETHOD MLA-060 Rev 10 Form 3D PERFLU O RIN ATED O RGAN ICS INITIAL CALIBRATION RETENTION TIMES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 10-Jan-2013 C S0 Data Filename: CS1 Data Filename: FC3G_015 S: 4 FC3G_015 S: 5 Instrument ID: LC MS/MS C S2 Data Filename: FC3G_015 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC3G_015 S: 7 C S4 Data Filename: FC3G_015 S: 8 C S5 Data Filename: FC3G_015 S: 9 C S6 Data Filename: FC3G_015 S: 10 C S7 Data Filename: FC3G_015 S: 11 C S8 Data Filename: N/A LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS LAB FLAG1 CS0 CS1 5:12 6:22 7:06 7:33 8:03 9:15 8:24 7:20 5:10 6:21 7:06 7:29 8:03 9:13 8:21 7:20 RETENTION TIMES CS2 CS3 CS4 CS5 CS6 5:10 6:22 7:09 7:29 8:03 9:15 8:24 7:20 5:14 6:22 7:09 7:33 8:03 9:13 8:21 7:20 5:12 6:21 7:09 7:33 8:03 9:13 8:21 7:20 5:12 6:22 7:09 7:33 8:03 9:15 8:24 7:20 5:11 6:20 7:06 7:29 8:03 9:13 8:21 7:20 CS7 5:12 6:21 7:06 7:29 7:59 8:21 7:20 CS8 MEAN RT 5:12 6:21 7:08 7:31 8:03 9:14 8:22 7:20 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form3D.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_10-Jan-2013_FC3G__Form3D_GS48596.html; Workgroup: WG42428; Design ID: 1980 ] Page 1 of 1 (WG42428 - PFOA_FC_LC_10-Jan-2013_FC3G__Form3D_GS48596.html) Page 97 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 4A PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 10-Jan-2013 V E R Data Filename: FC3G_015 S: 16 Instrument ID: LC MS/MS A nalysis Date: 14-Jan-2013 L C Colum n ID: C18MS A nalysis Time: 23:39:26 COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 RETEN TION TIME 5:12 5:58 6:21 6:44 7:06 7:29 7:59 8:35 9:11 6:29 7:20 8:21 9:58 EXPECTED CONC. (ng) 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 40.0 40.0 40.0 20.0 CONC. FOUND (ng) 19.3 18.5 20.7 20.1 20.6 21.7 21.5 21.1 21.8 44.4 38.7 40.3 18.6 RECOVERY 96.7 92.7 104 101 103 108 108 106 109 111 96.8 101 93.1 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_FC3G_015S16__Form4A_SJ1552177.html; Workgroup: WG42428; Design ID: 1980 ] Page 1 of 1 (WG42428 - PFOA_FC_LC_FC3G_015S16__Form4A_SJ1552177.html) Page 98 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 4B PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 10-Jan-2013 V E R Data Filename: FC3G_015 S: 16 Instrument ID: LC MS/MS A nalysis Date: 14-Jan-2013 L C Colum n ID: C18MS A nalysis Time: 23:39:26 LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS LAB FLAG 1 RETEN TION TIME 5:11 6:21 7:06 7:29 7:59 9:11 8:21 7:20 EXPECTED CONC. (ng) 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 CONC. FOUND (ng) 12.0 12.3 36.4 12.3 11.1 10.9 18.8 20.1 R E C O V E R Y (%) 100 102 101 102 92.8 90.6 104 112 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_FC3G_015S16__Form4B_SJ1552177.html; Workgroup: WG42428; DesignID: 1980 ] Page 1 of 1 (WG42428 - PFOA_FC_LC_FC3G_015S16__Form4B_SJ1552177.html) Page 99 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 4A PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 10-Jan-2013 V E R Data Filename: FC3G_015 S: 36 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 L C Colum n ID: C18MS A nalysis Time: 05:18:50 COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 RETEN TION TIME 5:14 5:57 6:22 6:44 7:09 7:29 7:59 8:32 9:07 6:29 7:20 8:17 9:58 EXPECTED CONC. (ng) 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 40.0 40.0 40.0 20.0 CONC. FOUND (ng) 20.0 17.8 20.0 19.9 19.9 21.0 20.6 20.5 22.4 42.1 42.3 41.7 18.4 RECOVERY 100 88.9 99.8 99.3 99.5 105 103 103 112 105 106 104 92.1 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_FC3G_015S36__Form4A_SJ1552199.html; Workgroup: WG42428; Design ID: 1980 ] Page 1 of 1 (WG42428 - PFOA_FC_LC_FC3G_015S36__Form4A_SJ1552199.html) Page 100 of 307 www.axysanalytical.com AXYS METHOD MLA-060 Rev 10 Form 4B PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 10-Jan-2013 V E R Data Filename: FC3G_015 S: 36 Instrument ID: LC MS/MS A nalysis Date: 15-Jan-2013 L C Colum n ID: C18MS A nalysis Time: 05:18:50 LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C4-PFOS 18O2-PFHxS LAB FLAG 1 RETEN TION TIME 5:12 6:22 7:09 7:29 7:59 9:07 8:17 7:20 EXPECTED CONC. (ng) 12.0 12.0 36.0 12.0 12.0 12.0 18.0 18.0 CONC. FOUND (ng) 11.7 12.1 35.7 12.2 10.6 10.3 18.0 18.6 R E C O V E R Y (%) 97.2 101 99.3 102 88.3 85.7 99.8 104 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 16-Jan-2013 14:49:00; Application: XMLTransformer-1.13.28; ReportFilename: PFOA_FC_LC_FC3G_015S36__Form4B_SJ1552199.html; Workgroup: WG42428; DesignID: 1980 ] Page 1 of 1 (WG42428 - PFOA_FC_LC_FC3G_015S36__Form4B_SJ1552199.html) Page 101 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-1 ,R, Name: FC3G_015S023 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 01:38:17 PFBA FC3G 015S023 PFPeA FC3G_015S023 100- %- 5.95 3.51 e 3 l 15171 :I i. 5.60 : : 6. .34 ' ' .v: 0- 5.00 6.00 7.00 PFNA FC3G_015S023 100- 7:49 2.21 e3~% 8717 l l %- .6.63 7.00 8.00 PFDA FC3GJ315S023 8.05 100- 2.02e3 . 5255 %- ... , 10.59 ._\! ' . -. " 8.00 10.00 12.00 PFHxS-1 FC3G_015S023 7.33 100-8.0 8 e 2 ^ 2372 . %- PFHxS-2 FC3G 015S023 PFHxA FC3G_015S023 100 6.34 _ 1.06e4 50412 , %~ 5.86 o- 5.00 6.00 7.00 PFUnA FC3G 015S023 PFHxS-3 FC3G 015S023 7.00 8.00 PFHpA FC3G 015S023 6-73 __ 100- 1.21e4 ; 47023 ! 5.65 5.00 6.00 7.00 PFDoA FC3G_015S023 100 .-.7.53 9-18 3.80e2 12.08 1489 LfeAf ` 0 rr 8.00 ' >' 10.00 12.00 PFOS-1 FC3G O15S023 PFOA-1 FC3G__015S023 100- %- 7.09 /. 2.93e4 113445 i , 7,00 8.00 PFBS-1 FC3G_015S023 4100- 6.46 1.56e3 5869 % : i5.25 5.84 5.00 6.00 7.00 PFOS-2 FC3G_JD15S023 in n 8-17 100 A 1.34e3 PFOA-2 FC3GJD15S023 _ 7.09 100- i 7.32e3 \ 26233 %H 7.00 8.00 PFBS-2 FC3G_015S023 100- 6.49 5.80e2 } 2631 %- 4 4.70 5.58 ;;....; 5.00 6.00 7.00 PFOSA FC3G_015S023 1 0 0 - 7 -53 8.00 10.00 12.00 9'7\ 10.89 JL87 . I mir 8.00 10.00 12.00 PV by LEH 15-Jan~13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. m Page 102 of 307 www.axysanalytical.com Quantify Sam ple Report M a ssLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L1 8989-1 ,R, Name: FC3G JJ15S023 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 01:38:17 13C2-PFOA FC3G_015S023 7n 100-1 |"2 .0 7 e 5 I #826207 : / '... . min 7.00 8.00 13C4-PFBA FG3G__015S023 100- 5.19 i~2.21e4 ! 88535 %- 4.03 5.00 6.00 7.00 13C4-PFOA FC3G 015S023 100- |~ 7.9 0e4 J314694 %- J0 - J : .. min 7.00 8.00 13C2-PFHxA FC3G 015S023 13C4-PFOS-1 FC3G 015S023 1 00 - f 1.15e4 jj 45060 %~ m 0 - - J k L , . , , . ....... , min 8-00 10.00 12.00 13C5-PFNA FC3G 015S023 13C4-PFOS-2 FC3G 015S023 1 00 - f 9.34e3 | 37285 %tj 0 - 'l l ,:,,, i'T'l : I'". , min 8.00 10.00 12.00 13C2-PFDA FC3G_015S023 Tfl100- 8.05 8.09e4 287382 %- 8.00 10.00 12.00 1802-PFHxS-1 FC3G_015S023 1 0 0 - 1.01 e4 1 42374 | | %~ 0J 7.00 8.00 13C2-PFDoA FC3G 015S023 min 1802-PFHxS-2 FC3G_015S023 100^7,70.23e33 J 29514 f t \ 7.00 8.00 13C2-PFOUEA FC3G 015S023 PV by LEH 15-Jan~13, SV b y A P 16-Jan~13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 103 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-1 ,R, Name: FC3G_015S023 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 01:38:17 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFN.A 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.21 26 262.98 > 218.... 5.95 26 312.97> 268.98 6.34 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.49 28 512.96 > 468.... 8.05 28 562.96 >518.... 8.73 29 612.95 >568,... 9.18 23 298.94> 79.96 6.46 24 298.94 > 98.95 6.49 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.29 23 398.84> 118.70 7.38 21 498.93> 79.96 8.17 22 498.93> 98.96 8.17 21 498 > 78 20 414.97 > 369.... 7.09 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.19 30 314.97>269.98 6.33 30 467.96>422.97 7.49 30 514.96 >469.96 8.05 30 614.95 > 569.... 9.18 458.98 >393.98 7.38 Area 9391 3512 10589 12095 29302 7320 2209 2021 721 380 1564 580 808 267 172 3069 1341 206795 78970 11475 9344 10069 7019 22121 49932 71635 80908 124386 27164 IS Area 22121 49932 49932 49932 206795 206795 71635 80908 80908 124386 10069 7019 10069 7019 10069 11475 9344 11475 78970 27164 27164 27164 27164 27164 27164 27164 27164 27164 ng/L 8.816 2.221 3.826 4.635 9.138 9.613 0.366 2.700 2.082 1.098 1.118 0.961 7.593 4.697 63.137 22.358 33.233 34.061 35.283 33.208 15.793 18.023 22.976 20.627 20.253 18.801 LOD 0.0806 0.2323 0.1745 0.1219 0.1192 0.3180 0.1368 0.2198 1.0415 0.4610 0.7750 0.1197 0.0649 0.0563 0.1072 %Rec S/N 62 20 85 38 124 297 11 15 5 5 28 67 23 135 6 36 44 92.1 97.8 96.9 99.3 102.9 96.8 69.1 78.8 100.5 90.2 88.6 98.7 597 0 747 484 112 237 64 548 1078 1019 648 0 Noise 610 743 591 1236 912 88 759 358 337 322 208 39 104 7 110 203 61 17 1384 0 60 77 380 124 1387 424 259 282 841 0 Height 37725 15171 50412 47023 113445 26233 8717 5255 1564 1489 5869 2631 2372 963 685 7347 2658 826207 314694 45060 37285 42374 29514 88535 232416 278949 287382 544719 104121 IS Height 88535 232416 Sample size 0.5250 0.5250 232416 0.5250 232416 826207 0.5250 0.5250 826207 0.5250 278949 0.5250 287382 0.5250 287382 0.5250 544719 0.5250 42374 29514 42374 0.5250 0.5250 0.5250 29514 0.5250 42374 0.5250 45060 0.5250 37285 45060 0.5250 0.5250 314694 0.5250 0.5250 104121 0.5250 104121 0.5250 104121 0.5250 104121 0.5250 104121 104121 0.5250 0.5250 104121 104121 0.5250 0.5250 104121 0.5250 0.5250 Factor2 Detection... 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMI 1.0 MMI 1.0 MMI 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 104 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\inst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-2,R, Name: FC3G_015S024 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 01:55:18 PFBA FC3G_015S024 100 _ 5.21 I 1.12&4 II 51174 %- 4.03 0- 5.00 6.00 7.00 PFNA FG3G 015S024 PFPeA FC3G_015S024 100- 5.93 4.03e3 !. 16979 ;; 6.53 4.03 5.41 . V' 5.00 6.00 7.00 PFDA FC 3 G _ 0 15 S 024 100-} _ 7:99 2.24e3 | 8620 %- h 8.79 / 10.62 PFHxA FC3G_015S024 100- 6.34 _ 1.02e4 44450 %- I 4.26 5.69 0- 5.00 6.00 7.00 PFUnA FC3G_015S024 100- 8.61 4.25e2 I 1698 12.17 %- Mi 7WL11.54.. ,A 10.08 /Tv PFHxS-1 FC3G__015S024 100- 7.33 -9.17e2~I 3332 m % 16.63 ok 7.00 8.00 8.00 10.00 12.00 8.00 10.00 12.00 PFHxS-2 FC3G_015S024 100- 7.29 (\ 4.48e2 1962 %- j6 .8 6 0- 7.00 V. 7.96 " 8.0 0 min PFHxS-3 FC3G 015S024 100- 7.29 f\ 3.51 e2 \ 1287 %-- 6.63 / L ...L o- 7.00 8.00 PFHpA FC3G_015S024 100- 6.73 8.94e3~| 35269 i' %" -l11l 5.54 .r;..i.\ irnivi;;;L min 5.00 6.00 7.00 PFOA-1 FC3G_015S024 10- frJ3i.1a9Le4 / \122202 7.00 8.00 PFDoA FC3GJJ15S024 100- 7.53 %- _ 9.21 | 5.59e2 1 2 . 1 4 I 1671 ! JV PFBS-1 FC3G015S024 100- 6.46 8.19c3 s 35644 J ! PFOA-2 FC3G_015S024 100-i _ 7.09 \ 8.22e3 \ 29542 %1 7.00 8.00 PFBS-2 FC3G015S024 100: 6.46 3.97e3~'! 18704 8.00 10.00 12.00 5.00 6.00 7.00 5.00 PFOS-1 FC3G 015S024 100- 8.29 |~ 7.18e3 3 17666 %- 0 - Mf#P?rTMrrrrtT7iTr7r r r min 8.00 10.00 12.00 PFOS-2 FC3G 015S024 100- 8.29 h 3.92e3 H 11005 ji I\ l-- 8.00 11.54 mi n 10.00 12.00 PFOSA FC3G 015S024 100- 9.96 1.49e2 1 743 ! %- A9.24 J 12.29 o: A- 8.00 10.00 12,00 PV byLEH 15-Jan-13, SV byAP 16-Jan-13. NOTE: OPRConcn. & LOD units = ng/mL, Sample and Lab BLKConcn. & LOD units = ng/L. Page 105 of 307 www.axysanalytical.com Q uantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\inst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-2,R, Name: FC3G__015S024 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 01:55:18 13C2-PFOA FC3G_015S024 100- _ 7.09 i 2.25e5 1949408 13C4-PFOA FC3G 015S024 100n 7.09 f~ 8.13e4 f l 335385 7.00 8.00 13C4-PFBA FC3G_015S024 100-, 5.21 I 2,58e4 1 121982 I 4.11 5.00 6.00 7.00 7.00 8.00 1302-PFHxA FC3G_015S024 100- 6.34 5.04e4 244534 , %- 5.00 6.00 7.00 13C4-PFOS-1 FC3G_015S024 100-, _ 8.35 1 1.16e4 | 43554 %--! I 13C4-PFOS-2 FC3G_015S024 100- 8.29 ip 9.53e3 1 36221 %- 8.00 10.00 12.00 8.00 10.00 12.00 13C5-PFNA FC3G 015S024 100- 7.49 7.85e4 A 335922 ; %-_ i /0 - ' ' - min 7.00 8.00 13C2-PFDA FC3G 015S024 7.99 1 0 0 " f 8.94e4 J 330014 % - f 1; i o: : -U 8.00 10.00 12.00 l8 0 2 -P F H x S -1 FC3G_015S024 100- 7.33 - 1,05e4~j| '41126 M %- 1802-PFHxS-2 FC3G 015S024 min 7.00 8.00 13C2-PFDOA FC3G_015S024 100- 9.18 1.41e5 612711 13C2-PFOUEA FC3G_015S024 100- 7.38 n2.71e4 'A 1057777 iLiA %- 8.00 10.00 12.00 7.00 8.00 PV by LEFI 15~Jan~13, SV by AP 16-Jan-13. NOTE: O P R Conen. & LOD units = ng/m L, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 106 of 307 www.axysanalytical.com Quantify Sam ple Report M a ssLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-2.R, Name: FC3GJ315S024 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 01:55:18 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-1 24 1802~PFHxS-2 25 13C4-PFBA 26 13C2-PFHXA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168 98 5.21 26 262.98 >2 1 8.... 5.93 26 312.97> 268.98 6.34 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.49 28 512.96 >468.... 7.99 28 562.96 > 518.... 8.61 29 612.95 > 568.... 9.21 23 298.94> 79.96 6.46 24 298.94 > 98.95 6.46 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.29 23 398.84> 118.70 7.29 21 498.93> 79.96 8.29 2 2 498.93> 98.96 8.29 21 498 > 78 9.96 2 0 414.97 > 369.... 7.09 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.29 30 402.9>84 7.33 30 402.9>103 7.29 30 216.99>171.98 5.21 30 314.97>269.98 6.34 30 467.96>422,97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.18 458.98 >393.98 7.38 Area 11163 4028 10221 8937 31931 8223 2098 2236 425 559 8187 3974 917 448 351 7179 3921 149 224822 81315 11560 9534 10526 7629 25804 50396 78491 89362 140512 27128 IS Area 25804 50396 50396 50396 224822 224822 78491 89362 89362 140512 10526 7629 10526 7629 10526 11560 9534 11560 81315 27128 27128 27128 27128 27128 27128 27128 27128 27128 ng/L 10.401 2.933 4.210 3.882 10.596 11 501 0.311 20.869 18.826 1.485 2 .1 1 1 2.793 22.036 16.592 0.424 77.111 26.630 38.781 40.255 42.725 41.811 21.338 21.070 29.160 26.388 26.501 21.720 LOD 0.1671 0.0315 0.0697 0.1385 0.2329 0.3833 15.6997 0 .2 0 0 2 0,1017 0.0908 0.1044 %Rec 97.2 100.7 97.8 101.5 107.7 105.4 80.7 79.7 110.3 99.8 1 0 0 .2 98.6 S/N 95 30 85 51 99 70 10 27 4 7 277 386 88 0 19 122 74 44 0 0 483 307 0 0 5 393 944 826 851 0 Noise 538 563 521 691 1241 420 891 316 384 251 129 48 38 0 68 145 149 17 0 0 90 118 0 0 24676 622 356 400 720 0 Height 51174 16979 44450 35269 122202 29542 8732 8620 1698 1671 35644 18704 3332 1962 1287 17666 11005 743 949408 335385 43554 36221 41126 30156 121982 244534 335922 330014 612711 105777 IS Height Sample size 121982 0.4538 244534 0.4538 244534 0.4538 244534 0.4538 949408 0.4538 949408 0.4538 335922 0.4538 330014 0.4538 330014 0.4538 612711 0.4538 41126 0.4538 30156 0.4538 41126 0.4538 30156 0.4538 41126 0.4538 43554 0.4538 36221 0.4538 43554 0.4538 335385 0.4538 0.4538 105777 0.4538 105777 0.4538 105777 0.4538 105777 0.4538 105777 0.4538 105777 0.4538 105777 0.4538 105777 0.4538 105777 0.4538 0.4538 Factor2 Detection... 1.0 MM 1.0 MM 1 .0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 MMi 1.0 bbi 1.0 bb! 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1 . 0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1 . 0 bb 1.0 bb 1 . 0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: O PR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 107 of 307 www.axysanalytical.com Quantify Sample Report M a ssLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-3.R, Name: FC3G_015S025 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 02:12:11 PFBA FC3G 015S025 100 5.24 I~7.91e3 Si 32099 ; i 5.77 4.03 ; v. , 6.57 PFPeA FC3G_015S025 100- 5.95 2.76e3 10979 ; %- M5.51 p 6.47 5.02 N 5.00 6.00 7.00 PFNA FC3G 015S025 5.00 6.00 7.00 PFDA FC3G_015S025 100 8.05 r 1.59e3 I 6020 %- 9-90 12j f PFHxS-1 FC3G 015S025 1 0 0 - 7,33 4.05e2~| 1597 if, %-: 6.76 / : / 0-- 7.00 7.90 ----- min 8.00 8.00 10.00 12.00 PFHxS-2 FC3G 015S025 100- 7.38 a 1.33e2 l 360 % - 6.63 / / \ 07.00 8.00 PFHxA FC3G_015S025 m n 6 -3 4 100 5.45e3 J 24581 %" 536 5 36 5 .8 2 :jll 5.00 ' 6 .0 0 '7 .0 0 PFUnA FC3G 015S025 PFHxS-3 FC3G_015S025 100n-1.70.233e2~l ` 554 %- 7.00 8.00 PFHpA FC3G 015S025 PFOA-1 FC3G_015S025 100- 7.09 A 9.37e3 \ 36406 %- PFOA-2 F.C3G 015S025 100- 7.09 ^ 2 .5 0 e 3 T 10036 % PFDoA FC3G_015S025 q 12 10(^ rS53e2 | 2124 %- a7.64 1 11.25. 12.23 b ma a k A '7 'v ' 0A l V , , , r r r ,rrrrrV l , i ; ,, r 8.00 10.00 12.00 PFOS-1 FC3G_015S025 8.17 100n i~ 2.42e3 1 5868 %- II 1n 1 Bm 10.6812.05 n 8.00 10.00 12.00 7.00 8.00 PFBS-1 FC3G_015S025 100- 6.51 1 .2 4 e 3 l 4816 ii %5.75 | \ 0 513 ""'t.L 5.00 6.00 7.00 P FO S-2 FC3G_015S025 100- 8.35 f 1.24e3 H 3550 %- 11.42J 1 ;8 1 8.00 10.00 12.00 7.00 8.00 PFBS-2 FC3G 015S025 100- 6.46 5 .1 5 e 2 l 2218 j| %- 1 4; 18 5.65 ; \ 5.00 ' 6 .0 0 ' 7.00 oo uJ PFOSA FC3G 015S025 9.96 8.28e1 ~) 2 71 |8 %- Lj 8.55 | o: ' ( I J ` 8.00 10.00 12.00 PV by LEH 15-Jan~13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab B LK C oncn. & LOD units = ng/L. Page 108 of 307 www.axysanalytical.com Q uantify Sample Report M assLynx 4.1 SCN627 Dataset: G :\M a s s ly nx \In s t_G \P F C .P R O \F C \F C 3 G _ 015~B.qid Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-3,R, Name: FC3G__015S025 D escription: 1,WG42428,15/40OOuL Date: 15-Jan-2013 Time: 02:12:11 13C2-PFOA FC3G 015S025 100" 7.09 IT 2.30 e5 /J915197 %: 13C4-PFOA FC3G 015S025 lo o n 7.09 f 8.87e4 | \ 362583 %~ 07.00 n min 8.00 07.00 8.00 13C4-PFBA FC3G_015S025 100- 5.21 I 2.68e4 I 106913 4.03 min 5.00 6.00 7.00 13C2-PFHxA FC3G_015S025 100- 6.34 5.79e4 268128 % 05.00 6.00 7.00 13C4-PFOS-1 FC3G__015S025 1 0 0 -n 8.35 1.32e4 47970 %~ 0 8.00 10.00 12.00 13C5-PFNA FC3G 015S025 100- ~~ 7.49 8.09e4 A 326335 i ' 7.00 8.00 13C4-PFOS-2 FC3G_015S025 100--| 8.35 1.05e4 42302 %-- 8.00 10.00 12.00 13C2-PFDA FC3G 015S025 100- 8.05 F 8.79e4 fj 307851 % - jl a iS iI V i 8.00 10:00 . 12.00 1802-PFHxS-l FC3G 015S025 7.33 100J 1.07e4 A 44341 A %~ /\ o- 7.00 8.00 13C2-PFDoA FC3G 015S025 100- 9.18 f 1.41 e5 r 608451 %: ]\ j v_ 8.00 -. oo :N :- oo :d . 1802-PFHxS-2 FC3G_015S025 7.33 100- 8.18e3~l 32319 f I %" 0 8.00 13C2-PFOUEA FC3G__015S025 7.38 100 3.15e4 ~p. 122324 j \ % 0 7.00 8.00 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: O PR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 109 of 307 www.axysanalytical.com Quantify Sample Report M a ssLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-3.R, Name: FC3G_015S025 Description: 1,WG42428,15/4000uL Date: 15Jan-2013 Time: 02:12:11 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFFIxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-l 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.9S>168.98 5.24 26 262.98 >218.... 5.95 26 312.97> 268.98 6.34 26 362.97> 318.98 6 .6 8 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 > 418.... 7.49 28 512.96 >468.... 8.05 28 562.96 > 518.... 8,55 29 612.95 > 568.... 9.12 23 298.94> 79.96 6.51 24 298.94 > 98.95 6.46 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.38 23 398.84> 118.70 7.33 21 498.93> 79.96 8.17 22 498.93> 98.96 8.35 21 498 > 78 9.96 20 414.97 > 369.... 7.09 416.97 >371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.21 30 314.97>269.98 6.34 30 467.96>422.97 7.49 30 514.96 >469.96 8.05 30 614.95 > 569.... 9.18 458.98 >393.98 7.38 Area 7907 2758 5446 3646 9373 2497 2019 1591 656 553 1244 515 405 133 102 2420 1237 83 230137 88685 13221 10475 10710 8180 26826 57928 80907 87897 141020 31495 IS Area 26826 57928 57928 57928 230137 230137 80907 87897 87897 141020 10710 8180 10710 8180 10710 13221 10475 13221 88685 31495 31495 31495 31495 31495 31495 31495 31495 31495 ng/L 6.786 1 .6 6 8 1.639 1.243 2.563 3.084 0.257 1.959 1.631 0.422 0.313 5.484 4.271 0.256 70.666 28.359 37.302 37.197 36.558 37.700 18.656 20.369 25.279 21.829 22.368 24.621 LOD 0.0271 0.1897 0.0597 0.1550 0.1473 0.1577 0.3947 3.2835 0.0514 0.1139 0.0893 0.0522 %Rec S/N 50 16 48 15 54 50 12 18 5 6 22 50 0 16 91.2 109.8 96.3 96.0 94.4 97.3 72.3 78.9 97.9 84.5 86.6 114.4 12 34 20 9 1400 0 663 294 0 0 17 1418 0 518 834 1414 Noise 643 681 508 874 674 202 741 341 377 337 219 44 0 22 45 174 178 32 654 0 72 144 0 0 6113 189 0 594 729 86 Height 32099 10979 24581 13046 36406 10036 8847 6020 1741 2124 4816 2218 1597 360 554 5868 3550 271 915197 362583 47970 42302 44341 32319 106913 268128 326335 307851 608451 122324 IS Height Sample size 106913 0.4648 268128 0.4648 268128 0.4648 268128 0.4648 915197 0.4648 915197 0.4648 326335 0.4648 307851 0.4648 307851 0.4648 608451 0.4648 44341 0.4648 32319 0.4648 44341 0.4648 32319 0.4648 44341 0.4648 47970 0.4648 42302 0.4648 47970 0.4648 362583 0.4648 0.4648 122324 0.4648 122324 0.4648 122324 0.4648 122324 0.4648 122324 0.4648 122324 0.4648 122324 0.4648 122324 0.4648 122324 0.4648 0.4648 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 bb! 1.0 MM! 1.0 MMI 1.0 MM 1.0 bb 1.0 MM! 1 . 0 MM 1 . 0 MM 1 . 0 MM 1.0 bb 1 . 0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1 . 0 bb 1.0 MM 1:0 bb 1 . 0 bb 1 . 0 bb 1.0 ' bb PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 110 of 307 www.axysanalytical.com Q uantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-4,R, Name: FC3G_015S026 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 02:29:12 PFBA FC3G__015S026 100- *i f 7.54e4 1297473 M S' i ... 5.00 6.00 7.00 PFPeA FC3G 015S026 100- 2.08e4 > 92157 i: : o - , ............. --'i:. 5.00 6.00 - min 7.00 PFHxA FC3G 015S026 100- 1.31 e 5 l 549250 %- r '\ 0 - _ ....-----------~.--.u-_ min 5.00 6.00 7.00 PFHpA FC3G 015S026 100n . 1.31e5 1 487207 g %] 1\ 0 - --- r ~ r ~ T i : mi n 5.00 6.00 7.00 PFOA-1 FC3G 015S026 ioo-q : %" / 5.28e5 : '1929210 , V. : /\ 0J __................-- 7.00 ................. 8.00 PFNA FC3G 015S026 PFDA FC3G_015S026 100- 7.99 I 3;76e3 1 12635 %- r*2 m // 8 5 1...1.. .i\31 8.00 10.00 12.00 PFUnA FC3G,,015S026 100- _ 8.58 7.23e2 3066 %~ 1 / 49f`2A1/1.451>1P2.29 0- 8.00 10.00 12.00 PFDoA FC3G_015S026 100- _ 9.15 I 5.50e2 I 781 I 1830 M % i/ ' il \10.^.Nl^/ 08.00 10.00 12.00 PFBS-1 FC3G_015S026 100- 6.47 _ 6.23e4 ' 282535 %- JL^. 5.00 6.00 7.00 PFHxS-1 FC3G 015S026 PFHxS-2 FC3GJD15S026 100- 7.33 -6 .7 0 e 3 i : 2308711 %- PFHxS-3 FC3G 015S026 PFOS-1 FC3G 015S026 PFOS-2 FC3G__015S026 100 _ 8.29 i 8.06e4 | 261911 %- 7.00 8.00 08.00 10.00 12.00 PFOA-2 FC3G_015S026 _ 7.09 1001 . 1.44e5 1520304 %J qJ 7.00 8.00 PFBS-2 FC3G__015S026 100- 6.46 3.27e4 149571 %- 5.00 6.00 7.00 PFOSA FC3G_015S026 100n 9.99 9.76e3 37047 % J V. 8.00 10,00 12:00 PV by LEFI 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 111 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-4,R, Name: FC3G_015S026 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 02:29:12 13C2-PFOA FC3G 015S026 100- 7.09 J^2.02e 5 800235 %-- 13C4-PFOA FC3G 015S026 100- 7.09 7.99e 4 f l 318382 %- 13C4-PFOS-1 FC3G_015S026 8.29 1001 f~1.19e4 1 42833 %- o - __ ______ 7.00 -TT min 8.00 13C4-PFBA FC3G _015S 026 100- 5.19 | 2.55e4 1 101806 % 0- I J l S i l i w ___ ! - n min 7.00 8.00 13C2-PFHxA FC3G _015S 026 100-, 6.34 4.71 e 4 /: 207673 0- 8.00 10.00 12.00 13C5-PFNA FC3G__015S 026 100- 7.49 ' - 7.03e 4 A 291680 M %- 5.00 6 . 0 0 7.00 ;'vvr- ;-- min 5.00 6.00 7.00 7.00 8.00 13C4-PFOS-2 FC3G_015S026 100- _ 8.29 9.00e3 32452 %- jEbL. 8.00 10.00 12.00 13C2-PFDA FC3G _ 015S 026 100- _ 7.99 8.21 e 4 . 309631 % 8.00 10.00 12.00 1802-PFHxS-1 FC 3G JD 15S 026 100- "~7.33 - 1.09e 4 ^ 42294 / \ %: 7.00 8.00 13C2-PFDoA FC3G _ 015S 026 9.18 .{" 1. 30e 5 ' 558747 8.00 10.00 12.00 1802-PFHxS-2 FC3G _ 015S 026 100--7.77.313e3~l : 29812 ( I %- 7.00 8.00 13 C 2 -P FO U EA FC 3G__015S 026 100- 7.38 2.68e 4 {\ 105369/ ; 7.00 8.00 PV by LEH 15~Jan~13, SV by AP 16-Jan-13. NOTE: O PR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 112 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-4,R, Name: FC3G_015S026 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 02:29:12 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 1 0 PFDoA 11 PFBS-1 1 2 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFOA 2 1 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHxS-1 24 1802~PFHxS~2 25 13C4-PFBA 26 13C2-PFHXA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.21 26 262.98 > 218.... 5.95 26 312.97> 268.98 6.34 26 362.97> 318.98 6 . 6 8 19 412.97> 368.98 7,09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.49 28 512.96 >468.... 7.99 28 562.96 > 518.... 8.58 29 612.95 > 568.... 9.15 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.46 23 398.84 > 79.96 7.33 24 398.84> 98,96 7.33 23 398.84> 118.70 7.29 2 1 498.93> 79.96 8.29 2 2 498.93> 98.96 8.29 2 1 498 > 78 9.99 2 0 414.97 >369.... 7.09 416.97 >371.9 7.09 30 503 > 79.96 8.29 30 503 > 98.96 8.29 30 402.9>84 7.33 30 402.9>103 7.33 30 2 16.99> 171,98 5.19 30 314.97>269.98 6.34 30 467.96>422.97 7.49 30 514.96 >469,96 7.99 30 614.95 >569..., 9.18 458.98 >393.98 7.38 Area 75433 20797 131494 130827 527976 143802 6143 3761 723 550 62254 32705 12365 6701 4412 122132 80650 9756 202327 79861 11925 8998 10879 7713 25472 47122 70268 82085 130386 26812 IS Area 25472 47122 47122 47122 202327 202327 70268 82085 82085 130386 10879 7713 10879 7713 10879 11925 8998 11925 79861 26812 26812 26812 26812 26812 26812 26812 26812 26812 ng/L 75.544 17.018 66.144 64.724 210.014 236.561 2.008 0.168 165.632 162.367 35.453 34.967 42.374 387.878 379.699 20.349 72.348 26.780 41.444 39.359 45.747 43.793 21.821 20.410 27.045 25.112 25.476 21.980 LOD 0.1974 0.0530 0.5982 0.8081 0.1619 0.2850 0.2090 0.1810 0.7392 0.1151 0.0593 0.0561 0.0529 %Rec 89.1 98.9 10 2.1 96.9 1 1 2 .6 107.8 80.6 75.4 99.9 92.8 94.1 97.4 S/N 541 176 1049 751 0 0 0 28 10 6 2348 3466 395 0 91 2394 755 1320 0 0 399 519 0 714 90 597 1446 1289 1575 0 Noise 550 525 523 648 0 0 0 456 305 318 120 43 113 0 170 135 347 28 0 0 107 63 0 42 1130 348 202 240 355 0 Height 297473 92157 549250 487207 1929210 520304 21047 12635 3066 1830 282535 149571 44497 23087 15362 322936 261911 37047 800235 318382 42833 32452 42294 29812 101806 207673 291680 309631 558747 105369 IS Height Sample size 101806 0.4432 207673 0,4432 207673 0.4432 207673 0.4432 800235 0.4432 800235 0,4432 291680 0.4432 309631 0.4432 309631 0,4432 558747 0.4432 42294 0.4432 29812 0.4432 42294 0.4432 29812 0,4432 42294 0.4432 42833 0.4432 32452 0.4432 42833 0.4432 318382 0.4432 0.4432 105369 0.4432 105369 0.4432 105369 0.4432 105369 0.4432 105369 0.4432 105369 0.4432 105369 0.4432 105369 0.4432 105369 0.4432 0.4432 Factor2 Detection... 1 . 0 bb 1 . 0 MM 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 bb! 1 . 0 MMi 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 113 of 307 www.axysanalytical.com Quantify Sample Report M a ssLyn x 4.1 SCN 627 Dataset: G :\M a s s Iy nx \in s t_G \P F C .P R O \F C \F C 3 G _ 015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-5,R, Name: FC3G JJ15S027 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 02:46:05 PFBA FC3G_015S027 100 r 7542e3 i 27832 %- ; .4.03 \ 5:99 PFPeA FC3G_015S027 100- 5.92 2.52e3 | 9388 %- 5.47; 528 .. 5.00 6.00 7.00 5.00 6.00 7.00 PFNA FC3G 015S027 PFDA FC3G_015S027 7.99 100-1 jp2.22e3 I 7143 % i\ 12.11 PFHxS-1 FC3G_015S027 100 7.29 2.90e2 1114 7.00 8.00 8.00 10.00 12.00 PFHxS-2 FC3G_015S027 100j - 7.29 l"1*34e2 m 420 %: 6.86 i o7.00 8.00 PFHxA FC3G 015S027 PFHpA FC3G_015S027 100- 6.68 _ 3.83e3 5.54 12577 i' :. 403 5.00 6.00 7.00 PFUnA FC3G_015S027 100- _ 8.61 5.74e2 i i 57 1964 T 12.29 %- 9 45 y, K kfthAA/ v\vf A Vl PFDoA FC3G_015S027 9.15 2.19e2 8.00 10.00 12.00 PFHxS-3 FC3G 015S027 8.00 10.00 12.00 PFOS-1 FC3G_015S027 1001 f2880e3 | 6581 %- 0U 9.78 11.78 8.00 10.00 12.00 PFOA-1 FC3G_015S027 100- 7.09 I f 9.21 e3 m 33913 PFOA-2 FC3G_015S027 100- \2.77.50e93 \ 9536 %- 7.00 8.00 7.00 PFBS-1 FC3G 015S027 PFBS-2 FC3G_015S027 100 6-44 6.44 41.5801e72 114.15801e72 %- 4.13 5.64 o- 5.00 6.00 7.00 P FO S-2 FC3G_015S027 100- _ 8.29 1.27e3 3679 %- PFOSA FC3G_015S027 100- 10.02 1.75e1~J 141 %- 10.23 9.57 11.75 ---A............. ........... -- mm 8.00 10.00 12.00 min 8.00 10.00 12.00 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 114 of 307 www.axysanalytical.com Quantify Sam ple Report M a ssLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L 1 8989-5,R, Name: FC3G_015S027 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 02:46:05 oo ooI 1 ! 1 13C2-PFOA FC3G_015S027 7n 100- 2 .3 5 e 5 P 946377 !\ 7.00 n min 13C4-PFOA FC3G 015S027 100^ i "-9.05e4 1359917 %- 0- J 7.00 , m in 8.00 13C4-PFOS-1 FC3G 015S027 100n - %- |T .2 8 e 4 1 48840 1 il ., 0- min 8:00 10.00 12.00 13C4-PFOS-2 FC3G 015S027 ; 1.02e4 : 38856 %;; 0 - min 8.00 10.00 12.00 1802-PFHxS-1 FC3G 015S027 100- rTl.15e4 f i 42976 0- / 7.00 ' . . 7 min 8.00 1802-PFHxS~2 FC3GJDT5S027 100- 7.29 7.70e3 29346 o7.00 8.00 13C4-PFBA FC3G 015S027 5.19 F 2.90e4 i\ 115917 % A 4.03 ! A.. 0 5.00 6.00 min 7.00 13C2-PFHxA FC3G 015S027 100- 6.33 5.81 e4 l254795 %- ___________ : j A . ______ 5.00 6.00 7.00 13C5-PFNA FC3G_015S027 7.49 100-3 7.99e4 A. 321201 j'\ %- o7.00 8.00 13C2-PFDA FC3GJD15S027' 100- 7.99 ( 9.13e4 1 335839 %- ki 8.00 10.00 12.00 13C2-PFDoA FC3G_015S027 9.18 f 1-28e5 1 580059 8.00 10.00 12.00 1 3 C 2-P FO U EA FC3G 015S027 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Gonen. & LOD units = ng/L. Page 115 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SC N 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-5,R, Name: FC3G_015S027 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 02:46:05 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA. 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 1 0 PFDoA 1 1 PFBS-1 1 2 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFOA 2 1 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.23 26 262.98 > 218.... 5.92 26 312.97> 268.98 6.34 26 362.97> 318.98 6 . 6 8 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.49 28 512.96 > 468.... 7.99 28 562.96 > 518.... 8.61 29 612.95 > 568.... 9.15 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.44 23 398.84 > 79.96 7.29 24 398.84> 98.96 7.29 23 398.84> 118.70 2 1 498.93> 79.96 8.23 2 2 498.93> 98.96 8.29 2 1 498 > 78 1 0 .0 2 2 0 414.97 > 369.... 7.09 416.97 > 371.9 7.09 30 503 > 79.96 8.29 30 503 > 98.96 8.29 30 402.9>84 7.29 30 402.9>103 7.29 30 216.99>171.98 5.19 30 314.97>269.98 6.33 30 467.96>422,97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.18 458.98 >393.98 7.38 Area 7420 2524 5503 3833 9211 2750 2312 2223 574 219 1244 450 290 134 2803 1268 17 235029 90468 12804 10179 11461 7702 28967 58055 79867 91321 127744 29955 IS Area 28967 58055 58055 58055 235029 235029 79867 91321 91321 127744 11461 7702 11461 7702 11461 12804 10179 12804 90468 29955 29955 29955 29955 29955 29955 29955 29955 29955 ng/L 6.583 1.707 1.867 1.478 2.754 3.776 0.409 1.967 1.653 0.524 7.656 5.110 0.158 79.692 32.587 42.786 42.808 46.335 42.042 23.859 24.177 29.554 26.861 23.998 26.379 LOD 0.1613 0.1573 0.1623 0.2527 0.3480 0.6170 0.7047 0.2656 0.1427 0.0853 0.1136 0.1093 0.0477 %Rec S/N 32 18 42 30 90 218 19 30 7 5 23 25 0 0 91.3 1 1 2 .0 98.1 98.1 106.2 96.4 82.0 83.1 1 0 1 .6 92.4 82.5 108.8 26 23 6 0 0 509 370 222 179 283 587 1099 686 786 1659 Noise 881 532 529 418 378 44 520 236 278 255 211 74 0 0 40 252 162 25 0 0 96 105 194 164 409 434 292 490 738 69 Height 27832 9388 21956 12577 33913 9536 9959 7143 1964 1197 4881 1817 1114 420 6581 3679 141 946377 359917 48840 38856 42976 29346 115917 254795 321201 335839 580059 113941 IS Height 115917 254795 254795 254795 946377 946377 321201 335839 335839 580059 42976 29346 42976 29346 42976 48840 38856 48840 359917 113941 113941 113941 113941 113941 113941 113941 113941 113941 Sample size 0.4126 0.4126 0.4126 0.4126 0,4126 0.4126 0.4126 0,4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 0.4126 Factor2 Detection... 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 MM 1 . 0 MMi 1 . 0 MMI 1 . 0 bbl 1 . 0 MM 1 . 0 MM 1 . 0 bbl 1 . 0 MM 1 .0 1 . 0 bb 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 116 of 307 www.axysanalytical.com Q uantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Massiynx\Inst_G\PFC.PRO\FC\FC3G_015-B.qid Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-6,R, Name: FC3G_015S028 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 03:03:06 PFBA FC3G_015S028 loo- I " 7.c70e3 f i 27663 i \A 4.05 5 71 min 5.00 6.00 7.00 PFNA FC3G_015S028 100-1 7.49 2.98e3~|| 10371 m %- 7.00 7.00 8.00 PFPeA FC3G 015S028 100%- 2.84 e3~) 11493 & 5.69 i : , :: 16.51 5.21... .. j V '. 0 - rr min 5.00 6.00 7.00 PFDA FC3G 015S028 7.99 1 0 0 - 2.10e3 jj 7898 %: 1 8.70 11.75. 12.35 0 -- 8.00 10.00 12.00 PFHxA FC3G 015S028 100%- 6.22e3 ' 25945 I \ . 5.62 5.82 05.00 6.00 7.00 PFUnA FC3G 015S028 8.55 10" r 33."5566ee22 1196 L 1 OTt 8.00 10.00 12.00 PFHxS-1 FC3G_015S028 PFHxS-2 FC3G 015S028 PFHxS-3 FC3G_015S028 100- 7.29 /. 6.43e1 h 319 %-- 6.90 l 7.00 8.00 PFHpA FC3G 015S028 100%~ 3.85e3 13766 5.49 i! ` PFOA-1 FC3G 015S028 100- \1.07e4 ! '\ 39726 %- 0 - .......................... .-I - min 5.00 6.00 7.00 0 - -------.........min 7.00 8.00 PFDoA FC3G_015S028 100- 12.29 11.87 I % i l '' o-----' ----- ------- r 8.00 10.00 12.00 PFBS-1 FC3GJ)15S028 100- 6.51 1.39e3 6346 c 7 -] 5.86 i 4.63 , - : 5.00 6.00 7.00 PFOS-1 FC3G_015S028 100- _ 8.29 2.72e3 I 5838 %- M 10-6511;36>2-20 ...v ~ : min 8.00 10.00 12.00 PFOS-2 FC3G_015S028 100- 8.29 " 1.70e3 4942 %- Q-- 8.00 11.31 11.99 10.00 12:00 PFOA-2 FC3G_015S028 100-j _ 7.09 1 2.71 e3 \ 9793 - %H 07.00 PFBS-2 FC3G 015S028 8.00 PFOSA FC3G_015S028 100- 9.84 _ 3.94e1 1 195 I I 8.05 9.151 11.93 J i __ A J U . 8.00 10.00 12.00 PV by LEH 15-Jan-13, SV by AP 16-Jan~13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 117 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G..015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-6,R, Name: FC3G_015S028 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 03:03:06 13C2-PFOA FC3G 015S028 100 J 7.09 2.50e5 976616 %-: 13C4-PFOA FC3G 015S028 ioo-q 7.09 J~ 9.46e4 f|3 7 1 8 5 0 %- ./ 0" \ 7.00 13C4-PFBA FC3G_015S028 100- 8.00 0^ i M 7.00 8.00 13C2-PFHxA FC3G_015S028 100- 6.34 _ 6.14e4 279226 h %- 5.00 6.00 7.00 13C4-PFOS-1 FC3GJD15S028 100- _ 8.29 1.45e4 56898 %' 8:00 10.00 12.00 13C5-PFNA FC3G 015S028 13C4-PFOS-2 FC3G 015S028 1 0 tEj 8.29 I 1.07e4 | 39210 %- PU 1802-PFHxS-1 FC3G 015S028 100^ 7.29 H"l.19e4 | | 44883 %~ II 0 J - ,, r r r r r r r r r r r r , min 8.00 10.00 12.00 0 ~J 7.00 8.00 13C2-PFDA FC3G_015S028 100_! f 1704e5 =! 372514 % 13C2-PFDoA FC3G_015S028 100- 9.15 r 1.45e5 1 654894 Q -- v--. - 8.00 10.00 12.00 J 8.00 10.00 12.00 1802-PFHxS-2 FC3G__015S028 100" _ 7.29 |8.85e3 132703 %- (H . 7.00 8.00 13C2-PFOUEA FC3G 015S028 PV by LEH 15~Jan~!3, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 118 of 307 www.axysanalytical.com Quantify Sample Report M a ssLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L1 8989-6,R, Name: FC3G_015S028 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 03:03:06 # Name 1 PFBA 2 PFPeA 3 PFHxA . 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 1 0 PFDoA 1 1 PFBS-1 1 2 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFOA 2 1 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 1302-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.19 26 262.98 >218.... 5,95 26 312.97> 268.98 6.34 26 362.97> 318.98 6 . 6 8 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.49 28 512.96 >468.... 7.99 28 562.96 >518.... 8.55 29 612.95 > 568.... 9.27 23 298.94> 79.96 6.51 24 298.94 > 98.95 6.46 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.29 23 398.84> 118.70 7.29 2 1 498.93> 79.96 8.29 2 2 498.93> 98.96 8.29 2 1 498 > 78 9.84 2 0 414.97 >369.... 7.09 416.97 >371.9 7,09 30 503 > 79.96 8.29 30 503 > 98.96 8.29 30 402.9>84 7.29 30 402.9>103 7.29 30 216.99>171.98 5.15 30 314,97>269.98 6.34 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 >569.... 9.15 458.98 >393.98 7.38 Area 7699 2840 6216 3854 10682 2709 2976 2099 356 582 1388 295 336 145 64 2715 1699 39 249649 94634 14515 10736 11858 8847 30269 61399 93584 104451 144829 33564 IS Area 30269 61399 61399 61399 249649 249649 93584 104451 104451 144829 11858 8847 11858 8847 11858 14515 10736 14515 94634 33564 33564 33564 33564 33564 33564 33564 33564 33564 ng/L 5.422 1.513 1 .6 8 6 1.159 2.543 2.883 0.410 1.854 0.513 0.398 5.264 5.521 0.161 67.157 28.289 35.923 33.442 35.506 35.769 18.466 18.938 25.649 22.755 20.151 24.529 LOD 0.0676 0.2633 0.0019 0.1191 0.4650 0.2274 0.1527 0.2528 0.6219 0.0876 0.1036 0.0915 0.0590 %Rec 92.7 117.2 99.2 92.4 98.1 98.8 76.5 78.5 106.3 94.3 83.5 1 2 2 .0 S/N 39 12 34 21 83 233 13 19 3 5 25 8 14 9 10 37 30 12 0 183 485 627 0 410 82 770 784 695 1211 0 Noise 709 941 763 669 480 42 826 419 402 319 254 154 84 52 31 159 166 16 0 2037 117 63 0 80 1301 362 482 536 541 0 Height 27663 11493 25945 13766 39726 9793 10371 7898 1196 1531 6346 1201 1207 478 319 5838 4942 195 976616 371850 56898 39210 44883 32703 106773 279226 378118 372514 654894 128491 IS Height Sample size 106773 0.4972 279226 0.4972 279226 0.4972 279226 0.4972 976616 0.4972 976616 0.4972 378118 0.4972 372514 0.4972 372514 0.4972 654894 0.4972 44883 0.4972 32703 0.4972 44883 0.4972 32703 0.4972 44883 0.4972 56898 0.4972 39210 0.4972 56898 0.4972 371850 0.4972 0.4972 128491 0.4972 128491 0.4972 128491 0.4972 128491 0.4972 128491 0.4972 128491 0.4972 128491 0.4972 128491 0.4972 128491 0.4972 0.4972 Factor2 Detection... 1 . 0 bb 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 MMI 1 . 0 MMI 1 . 0 MMI 1 . 0 MM 1 . 0 MM 1 . 0 MMI 1 . 0 MM 1 . 0 MMI 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 , 0 bb 1 . 0 bb PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 119 of 307 www.axysanalytical.com Quantify Sam ple Report M a ssLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRQ\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-7,R, Name: FC3G_015S029 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 03:19:59 PFBA FC3G 015S029 PFPeA FC3GJ15S029 5.97 100- 4.40e3 ?; 15975 PFHxA FC3G_015S029 100t 6.36 _ 1.22e4 57546 PFHpA FC3G_015S029 in n 6 J 3 100 1,42e4 56116 i? PFNA FC3G 015S029 PFHxS-1 FC3G 015S029 100- 7.33 9.53e2 ?; 4291 i \ 6.71 7.00 8.00 5.00 6.00 7.00 PFDA FC3G_015S029 100- 7.99 T 1.91e3 I 6562 %- 1 10.17. . ,, , . 12.20 8.00 10.00 12.00 PFHxS-2 FC3G_015S029 100-1 -6.63 A 7.29 f"4.05e2 \ 1041 \ 7.00 8.00 5.82: 5.00 6.00 7.00 PFUnA FC3G,,015S029 100- 8.61 r 5.32e2 1 1721 1? 32 5.00 6.00 7.00 PFDoA FC3G_015S029 100- 7.53 11.90 11.42 AI % 8.00 10.00 12.00 PFHxS-3 FC3G_015S029 100 7.33 -2 .0 8 e 2 l 961 A %- 6.76 I 7.00 8.00 8.00 10.00 12.00 PFOS-1 FC3G_015S029 100- 8.29 3.06e3 7032 %- o4 8.00 11.13 , 10.00 12.00 PFOA-1 FC3G 015S029 100" 7.09 ; 3.00e4 116292 %- PFOA-2 FC3G_015S029 100- 7.09 A 8.32e3 \ 30848 0/o/ - .j',, . ' _ 7.00 8.00 PFBS-1 FC3G 015S029 100- 6.51 1 .7 0 e 3 l 7855 n %: h 5.64.5.80 ; \ - -i 0J 5.00 6.00 7.00 PFOS-2 FC3G_015S029 100 8.29 f 1 ,76e3 1 4714 %- 1 0 ^ 11.36 1 2 -2 3 o- m in 8.00 10.00 12.00 7.00 8.00 PFBS-2 FC3G_015S029 100- 6.47 6.47 5 . 3 9 e 2 l 5.39e2 2458 5.115.25 7.07 j\; min 5.00 6.00 7.00 PFOSA FC3G_015S029 100 a8 1 11.72 ILk 8.00 10.00 12.00 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 120 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L1 8989-7,R, Name: FC3G_015S029 Description: 1,WG42428,15/4000uL Date: 15^Jan-2013 Time: 03:19:59 13C2-PFOA FC3G_015S029 100- 7n f '782413 --- i-- 7.00 -------- min 8,00 13C4-PFOA FC3G 015S029 100- A 8.07e4 .310817 %- 0^ . . ..... .. 7 ^.-= .--- min 7.00 8.00 13C4-PFOS-1 FC3G 015S029 100- '~~1.10e4 42623 %~ 1o - ,r l y 8.00 10.00 min 12.00 13C4-PFOS-2 FC3G 015S029 100- i8.63e3 ; 34121 %- : > 0 8.00 10.00 min 12.00 1802-PFHxS-1 FC3G 015S029 100- 8.89e3l| 33564 h %- :x 0 - ........; 7.00 -r min 8.00 1802-PFHxS-2 FC3G_0T5S029 100" 7.33 7.22e3~j 30184 l \ %-- - 0- J - 7.00 8.00 13C4-PFBA FC3G_015S029 100- 5.19 |~2.23e4 1 93809 %- i\ L<F i 24! ~rr min 5.00 6.00 7.00 13C2-PFHxA FC3G_015S029 100- 6.34 4.84e4 217636 1 %- 1 jj|^ t r min 5.00 6.00 7.00 13C5-PFNA FC3GJD15S029 100-, 7.49 7.17e4 f\ 293826 j \ %- o-: 7.00 8.00 13C2-PFDA FC3G_015S029 100- _ 7.99 7.92e4 292645 %- 13C2-PFDoA FC3G_015S029 100--j 9.15 F 1.11e5 | 501386 %- 13C2-PFOUEA FC3G__015S029 100- - 2 7.38 .7 6 e 4 'l .104469 h %- 8.00 10.00 12.00 8.00 10.00 12.00 7.00 8.00 PV by LEH 15-Jan-13., SV by AP 16-Jan-13. NOTE: O PR Conen. & LOD units = ng/m L, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 121 of 307 www.axysanalytical.com Quantify Sample Report M a ssLy n x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L 1 8989-7,R, Name: FC3G JJ15S029 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 03:19:59 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHXS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2~PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.23 26 262.98 >218.... 5.97 26 312.97> 268.98 6.36 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 > 418.... 7.49 28 512.96 >468.... 7.99 28 562.96 > 518.... 8.61 29 612.95 >568.... 9.18 23 298.94> 79.96 6.51 24 298.94 >98.95 6.47 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.29 23 398.84> 118.70 7.33 21 498.93> 79.96 8.29 22 498.93> 98.96 8.29 21 498 > 78 20 414.97 > 369.... 7.09 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 >98.96 8.29 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.19 30 314.97>269.98 6.34 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9,15 458.98 >393.98 7.38 Area 8723 4398 12179 14199 30040 8322 1970 1905 532 284 1704 539 953 405 208 3057 1757 197313 80701 11020 8633 8889 7222 22346 48407 71694 79226 111157 27586 IS Area 22346 48407 48407 48407 197313 197313 71694 79226 79226 111157 8889 7222 8889 7222 8889 11020 8633 11020 80701 27586 27586 27586 27586 27586 27586 27586 27586 27586 ng/L 8.610 3.083 4.926 6.014 10.506 12.273 0.306 3.833 1.941 1.953 1.848 1.626 8.437 7.314 62.838 24.354 33.499 33.030 32.696 35.865 16.745 18.340 24.136 21.200 18.998 20.353 LOD 0.1846 0.1657 0.2019 0.1270 0.1710 0.5264 0.1377 0.1539 0.5530 0.1558 0.0526 0.0578 0.1019 %Rec S/N 44 30 110 46 304 444 18 33 5 2 35 30 136 0 20 32 61 86.0 100.0 91.7 90.4 89.5 98.1 68.7 75.3 99.1 87.0 78.0 100.2 0 0 600 196 710 772 101 419 1490 1074 666 0 Noise 780 525 523 1212 382 69 437 200 353 445 227 82 32 0 47 220 78 16 0 0 71 174 47 39 932 519 197 272 752 0 Height 34477 15975 57546 56116 116292 30848 7914 6562 1721 840 7855 2458 4291 1041 961 7032 4714 782413 310817 42623 34121 33564 30184 93809 217636 293826 292645 501386 104469 IS Height Sample size 93809 0.4925 217636 0.4925 217636 0.4925 217636 0.4925 782413 0.4925 782413 0.4925 293826 0.4925 292645 0.4925 292645 0.4925 501386 0.4925 33564 0.4925 30184 0.4925 33564 0.4925 30184 0.4925 33564 0.4925 42623 0.4925 34121 0.4925 42623 0.4925 310817 0.4925 0.4925 104469 0.4925 104469 0.4925 104469 0.4925 104469 0.4925 104469 0.4925 104469 0.4925 104469 0.4925 104469 0.4925 104469 0.4925 0.4925 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1,0 MM 1.0 MMI 1.0 MMI 1.0 MMI 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 122 of 307 www.axysanalytical.com Quantify Sam ple Report M a ssLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-8,R, Name: FC3G_015S030 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 03:37:00 PFBA FC3G_015S030 100 5.23 ; 6.62e3 25681 PFPeA FC3G_015S030 100- 5.92 _ 2.93e3 8918 %- 5.38 5 92 2.93e3 8918 -ri > . 5.00 6.00 7.00 PFNA FC3G 015S030 PFHxS-1 FC3G 015S030 5.00 6.00 7.00 PFDA FC3G__015S030 inn . 7.99 2.21 e3 A 7132 M ; ; 10.65 12.23 : - : -4 r^TT r 8.00 10.00 12.00 PFHxS-2 FC3G 015S030 PFHxA FC3G 015S030 PFUnA FC3G_015S030 100-, 8.01 4.55e2 , 1260 1223 _ .... %- i'M ll ' v 0-Lt rrrp-rr-q-.-rnjrT f 8.00 10.00 12.00 PFHxS-3 FC3G_015S030 100_7.29;1.25e2;404 6.71 7.00 8.00 PFHpA FC3G_015S030 100n 6.73 3.23e3 ' 5.58,. 10476 I . % 4.03 '. 5.00 6.00 7.00 PFDoA FC3G_015S030 100- 12.20 7.53 11.45 V 11.07^ %- 8 55 , \' 8.00 10.00 12.00 PFOS-1 FC3G_015S030 100- 8.23 =2.72e3 l 5933 % rl 10.8012-20 !... ;j .. _ . V .' 8.00 10.00 12.00 PFOA-1 FC3G__015S030 100- 7.09 A 1.02e4 i 38004 % 7.00 PFBS-1 FC3G 015S030 PFO S-2 FC3G 015S030 100" 8.29 \ 1.52e3 ;; 4709 %: i! - !: n 8.00 1 0 -5 6 11.45 10.00 12.00 PFOA-2 FC3G_015S030 100- 7.09 A 2.43e3 . 9817 %- 7.00 8.00 PFBS-2 FC3G_015S030 100- 6.46 2 .6 4 e 2 l 1497 I %- 4.13 5.71 m 5.00 6.00 7.00 PFOSA FC3G_015S030 1 0 0 -, 9.84 5.0 6e11 236 I %- 9.18 _- A , 10.47 11.99 m in 8.00 10.00 12.00 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: O PR Concn. & LOD units = ng/mL, Sam ple and Lab BLK Concn. & LOD units = ng/L. Page 123 of 307 www.axysanalytical.com Quantify Sample Report M assLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-8,R, Name: FC3G_015S030 Description: 1,WG42428,15/4000uL Date: 15-Jan~2013 Time: 03:37:00 13C2-PFOA FC3G 015S030 100- 7.09 i 2.09e5 fe 858383 %- 0J 7.00 "tt min 8.00 13C4-PFOA FC3G 015S030 100- 7.09 JT 7.96e4 326778 o - J M l -T-T min 7.00 8.00 13C4-PFOS-1 FC3G 015S030 100- 8.35 r 1.22e4 46431 %- 0 - min 8.00 10.00 12.00 13C4-PFO S-2 FC3G 015S030 100- 8.29 f 9.95e3 ., 36608 %- i! 0 - ....... min 8.03 10.00 12.00 1802-PFHxS-1 FC3G 015S030 100m 7.33 1*03e4"| 39962 %- O-' 7.00 -h min 8.00 1802-PFHxS-2 FC3G 015S030 100- 7.29 fi7 .4 2 e 3 29224 /\ 0-J 7.00 8.00 13C4-PFBA FC3G 015S030 13C2-PFHxA FC3G 015S030 100- 6.34 5.31e4 ; 240482 M M %~ 13C5-PFNA FC3G 015S030 100- 7.49 7.73e4 A 319227 i \ %- 13C2-PFDA FC3G_015S030 100m 7.99 8.23e4 1 294697 %- 13C2-PFDoA FC3G 015S030 13C 2-P FO U EA FC3GJ15S030 100-- 7.38 2.80e4~J 109286 i o- ...... ~ min 5.00 6.00 7.00 o: 7.00 M1?`1 min 8.00 08.00 10.00 12.00 7.00 8.00 PV by LEH 15-Jan-13, SV b y A P 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 124 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 Dataset G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered; Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-8,R, Name: FC3G_015S030 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 03:37:00 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2~PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT Area IS Area ng/L 25 212.99>168.98 5.23 6624 24691 5.912 26 262.98 > 218.... 5.92 2926 53115 1.872 26 312.97> 268.98 6.34 5224 53115 1.673 26 362.97> 318.98 6.73 3233 53115 1.153 . 19 412.97> 368.98 7.09 10220 209046 3.071 19 412.97> 168.80 7,09 2433 209046 3.209 27 462.96 >4 1 8.... 7.49 1917 77334 0.243 28 512.96 >468.... 7.99 2211 82276 28 562.96 > 518.... 8.61 455 82276 29 612.95 >568.... 9.21 246 125798 23 298.94> 79.96 6,49 1137 10252 . 1.744 24 298.94 > 98.95 6.46 264 7424 0.607 23 398.84 > 79.96 7.33 306 10252 24 398.84> 98.96 7.29 108 7424 0.339 23 398.84> 118.70 7.29 125 10252 0.563 21 498.93> 79.96 8.23 2716 12197 6.678 22 498.93> 98.96 8.29 1523 9952 5.480 21 498 > 78 9.84 51 12197 0.198 20 414.97 > 369.... 7.09 209046 79648 68.815 416-97 >371.9 7.09 79648 24.522 30 503 > 79.96 8.35 12197 27972 37,305 30 503 > 98.96 8.29 9952 27972 38.309 30 402.9>84 7.33 10252 27972 37.939 30 402.9>103 7.29 7424 27972 37.092 30 216.99>171,98 5.21 24691 27972 18.615 30 314.97>269.98 6.34 53115 27972 20.247 30 467.96>422.97 7.49 77334 27972 26.194 30 514.96 >469.96 7.99 82276 27972 22.151 30 614.95 > 569.... 9.18 125798 27972 21.631 458.98 >393.98 7.38 27972 21.054 LOD 0.1861 0.7569 0.1190 0.3535 0.1278 0.1886 0.2183 0.3527 0.2239 0.0792 0.0894 0.0913 0.1015 %Rec 92.3 98.6 100.0 102.7 101,7 99.5 74.9 81.4 105.4 89.1 87.0 101.6 S/N 52 14 31 19 124 51 28 26 4 4 26 61 16 1 10 61 52 23 584 576 578 496 0 0 167 314 1048 682 810 622 Noise 494 619 747 554 306 194 322 270 314 194 158 24 63 251 40 97 91 10 1469 568 80 74 0 0 609 765 305 432 692 176 Height 25681 8918 23013 10476 38004 9817 8993 7132 1260 743 4034 1497 1000 286 404 5933 4709 236 858383 326778 46431 36608 39962 29224 101954 240482 319227 294697 560015 109286 IS Height Sample size .101954 0.4827 240482 0.4827 240482 0.4827 240482 0.4827 858383 0.4827 858383 0.4827 319227 0.4827 294697 0.4827 294697 0.4827 560015 0.4827 39962 0.4827 29224 . 0.4827 39962 0.4827 29224 0.4827 39962 0.4827 46431 0.4827 36608 0.4827 46431 0.4827 326778 0.4827 0.4827 109286 0.4827 109286 0.4827 109286 0.4827 109286 0.4827 109286 0.4827 109286 0.4827 109286 0.4827 109286 0.4827 109286 0.4827 0.4827 Factor2 Detection... 1.0 MM 1,0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MMI 1.0 MMI 1.0 MMI 1.0 MM 1.0 MM 1.0 MMI 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1,0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 125 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-9,R, Name: FC3G_015S031 D escription: 1,WG42428,15/4000uL Date: l 'Jan ^ O IS Time: 03:54:01 PFBA FC3G 015S031 PFPeA FC3G_015S031 100- 5.95 1.84e3lj 9847 I %" 5.43 j [ 6 -4 2 4.07 PFHxA FC3G_015S031 100- 6.33 _ 4.86e3 19900 PFNA FC3G 015S031 PFHxS-1 FC3G_015S031 100-- 7.33 2.58e2 _ I 1114 | | % 6 63 7.00 8.00 5.00 6.00 7.00 5.00 6.00 7.00 PFDA FC3G_015S031 -inn 7 -99 1GOn ITM1.66e3 I 5969 %| \ 8/ 6 4 11.36 1/ 2 ` 1 4 mTmmrTTri nnivn.i min 8.00 10.00 12.00 PFUnA FC3G_015S031 100- _ 8.58 3.92e2 | 2115 12.02 %- 11.39^ 9.421 0 -1" ....... ...... ... 11-tttttt r 8.00 10.00 12.00 PFHxS-2 FC3G_015S031 7.29 1 0 0 1.1502 I 448 %- 16.63 ; \ L::.n 7.00 8.00 PFHxS-3 FC3G 015S031 PFHpA FC3G_015S031 100- 6.68 3.40e3 / 10828 j I 5.391 '\ PFOA-1 FC3G_015S031 100- 7.09 \ 8.83e3 \ 34248 %- PFOA-2 FC3G_015S031 100 _ 7.09 \ 1.99e3 .! 7345 5.00 6.00 7.00 7.00 8.00 7.00 8.00 PFDoA FC3G_015S031 100 10.86 11-81 9 .5 7 ) ,I ft ,8.11 1|| HI I 8.00 10.00 12.00 PFBS-1 FC3G_015S031 100n 6.53 _ 1.04e3 4429 ! % 5.80 j ! 5`67' - . -I \ 0-^~" - .. min 5.00 6.00 7.00 PFBS-2 FC3G_015S031 mn 100n 6.44 3.14e2T- e-4 4 1490 I1 3 1' 1449e02 %- 11 6.97 5.775.90 AM/H, ^\ ^ 5.00 6.00 7.00 PFOS-1 FC3G_015S031 100-j 8,23 2.39e3 5048 %- o4*l 8.00 l l .31/11.90 min 10.00 12.00 PFOS-2 FC3G_015S031 100-. 8.29 IT 1.34e3 I 3545 Vo 11.01 11.90 Aw \ AAy. 8.00 10.00 12.00 PFOSA FC3G__015S031 -inn 9 -81 10-> 2 .2 3 e ll 137 j gel %- IT 2.23e1 Is 137 12.20 .7.64 1 10.68 S 0- A c " min 8.00 10.00 12.00 P V by LEH 15~Jan~13, S V by A P 16 -Jan -13 . N O T E : O P R Conen. & LO D units = ng/m L, S am p le and Lab BLK Conen. & LO D units = ng/L. Page 126 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4,1 SCN627 Dataset: G :\M a sslynx\ln stj3\P F C .P R O \F C \F C 3G _0 15-B .q ld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-9,R, Name: FC3G_015S031 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 03:54:01 13C2-PFOA FC3G_015S031 100-1 IT 27.3r7e5 943761 i A A. ' - 'V . i r r r t min 7.00 8.00 13C4-PFOA FC3G 0.15S031 100^ %- C 8.4 4 e4 M 331164 0- J Vv , T-T min 7.00 8.00 13C4-PFOS-1 FC3G 015S031 100- [* 1,26e4 1 48642 %-- li 0 - min 8.00 10.00 12.00 13C4-PFOS-2 FC3G 015S031 100- S 1.01e4 37938 %- ! J 0 - min 8.00 10.00 12.00 1802-PFHxS~1 FC3G 015S031 100- I "l.03e4 j jj 40930 %~ 0L 7.00 1802-PFH xS"2 FC3G_015S031 100n 7.29 /I"8.44e3 34133 %- mjn 07.00 13C4-PFBA FC3G_015S031 100- 5.19 ft 2.61e4 113811 %- i i 4.05 j 5.00 6.00 7.00 . 13C2-PFHxA FC3G_015S031 100- 6.33 5 .7 2 e4 l 248070 | %z j\ 5.00 6.00 7.00 13C5-PFNA FC3G_015S031 100- 7.49 8.18e4 A 329750 j \ %- 7.00 8,00 13C2-PFDA FC3G 015S031 100- 7.99 8,63e4 i 323998 %- Q--Mi k, 8.00 10.00 12.00 13C2-PFDoA FC3G__015S031 8.00 10.00 12.00 13C2-PFOUEA FC3G_015S031 100--3 7.38 .11e4 A ,115425/ \ %- 7,00 8.00 PV by LE H 15 -Jan -13 , S V by A P 16~Jan~13. N O T E : O P R Conen. & LO D units = ng/m L, S am p le and Lab BLK Conen. & LO D units = ng/L. Page 127 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-9,R, Name: FC3G_015S031 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 03:54:01 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFB5-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHX5-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-l 24 1802-PFHX5-2 25 13C4-PFBA 26 13C2-PFHXA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDOA 30 13C2-PFOUEA . IS# Trace RT 25 212.99>168-98 5.19 26 262.98 >218.... 5.95 26 312.97> 268.98 6.33 26 362.97> 318.98 6 . 6 8 19 41 2 .97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.49 28 512.96 > 468.... 7.99 28 562.96 >518.... 8.58 29 612.95 >568.... 9.21 23 298.94> 79.96 6.53 24 298.94 >98.95 6.44 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.29 23 398.84> 118.70 7.29 21 498.93> 79.96 8.23 22 498.93> 98.96 8.29 21 498 > 78 9.81 20 414.97 > 369.... 7.09 416.97 >371,9 7.09 30 503 > 79.96 8.29 30 503 > 98.96 8.29 30 402.9>84 7.29 30 402.9>103 7.29 30 216.99>171.98 5.19 30 314.97>269.98 6.33 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 >569.... 9.15 458.98 >393.98 7.38 Area 6366 1844 4862 3400 8826 1988 1767 1660 392 351 1036 314 258 115 110 2387 1343 22 236915 84418 12591 10093 10307 8442 26130 57166 81775 86250 128225 31072 IS Area 26130 57166 57166 57166 236915 236915 81775 86250 86250 128225 10307 8442 10307 8442 10307 12591 10093 12591 84418 31072 31072 31072 31072 31072 31072 31072 31072 31072 ng/L 6.094 1.21 1.580 1.283 2.526 2.536 0.185 1.676 0.760 0.352 0.472 6.297 5.371 0.165 84.103 29.707 39.626 39.979 39.246 43.400 20.270 22.422 28.500 23.893 22.687 26.731 LOD 0.1386 0.0794 0.1347 0.2825 0.3662 0.9936 0.3201 0.1810 0.1105 0.0566 0.1310 0.0122 %Rec 98.7 104.6 93.0 93.8 92.1 1 0 1 .8 71.3 78.9 100.3 84.1 79.8 112.9 S/N 61 20 46 13 160 77 10 23 9 3 73 16 17 10 13 48 23 14 0 0 438 331 0 143 223 434 840 1281 584 6593 Noise 391 488 428 855 214 95 773 254 231 290 61 95 64 45 45 105 155 10 0 0 in 114 0 239 511 572 393 253 917 18 Height 23712 9847 19900 10828 34248 7345 7941 5969 2115 998 4429 1490 1114 448 578 5048 3545 137 943761 331164 48642 37938 40930 34133 113811 248070 329750 323998 535252 115425 IS Height Sample size 113811 0.4224 248070 0.4224 248070 0.4224 248070 0.4224 943761 0.4224 943761 0.4224 329750 0.4224 323998 0.4224 323998 535252 0.4224 0.4224 40930 0.4224 34133 0,4224 40930 0.4224 34133 0.4224 40930 0.4224 48642 0.4224 37938 0.4224 48642 0.4224 331164 0.4224 0.4224 115425 0.4224 115425 0.4224 115425 0.4224 115425 0.4224 115425 0.4224 115425 . 0.4224 115425 0.4224 115425 0.4224 115425 0.4224 0.4224 Factor2 Detection... 1.0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 MMI 1.0 MMi 1.0 MMI 1.0 MM 1.0 MM 1.0 MMi 1.0 MM 1 . 0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb P V by LE H 15 -Jan -13 , S V by A P 16 -Jan -13 . N O T E : O P R C onen. & LO D units = ng/m L, S am p le and Lab BLK C onen. & LO D units = ng/L. Page 128 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G :\M as s ly nx \In s t_G \P F C .P R O \F C \F C 3 G _ 015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-10.R, Name: FC3G_015S032 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 04:10:54 PFBA FC3G__015S032 100- 5.26 (T 6.63e3 I 24363 %" :4.03 PFPeA FC3G_015S032 100- 5.99 1.77e3~| 8584 tt % 5.64. j j , 6.53 14.03 / \ f t v ( 5.00 6.00 7.00 PFNA FC3G 015S032 PFHxS-1 FC3G 015S032 5.00 6.00 7.00 PFDA FC3G_015S032 100- 7.99 |T 1,61e3 M 4522 10.8312.17 \ \/ 0-v 8.00 10.00 12.00 PFHxS-2 FC3G 015S032 PFHxA FC3GJJ15S032 100- 6.36 _ 5.03e3 I 23492 1 %- 4.29 5.62 5.00 6.00 7.00 PFUnA FC3GJ)15S032 100- 8.64 f 2.59e2 I 1194 11-57, %- 8.00 10.00 12.00 PFHxS-3 FC3G_015S032 100--1.74.83e32 A 645 n %- 7.00/ ! - " L..J 7.00 8.00 PFHpA FC3G 015S032 PFOA-1 FC3G,,015S032 100- 7.09 A 9.26e3 f \ 33791 %- 7.00 8.00 PFDoA FC3GJD15S032 9 12 100^ F290e2 , I 1641 : 7.64 %- II ,9.99 v/ PFBS-1 FC3GJD15S032 100n 6.51 8.27e2_ | 4555 II %- 5-49 5 .9 2 I tjtt min 8.00 10.00 12,00 5.00 6.00 7.00 PFOS-1 FC3G__015S032 100- 8.11 I 1.93e3 H 4282 %- : , 10-65 11.6 8.00 10.00 12.00 PFOS-2 FC3G_015S032 100- 8.29 /: 1.03e3 l \ 2493 %- 10.98..11.60 J....3.. - - - - - 8.00 10.00 12.00 PFOA-2 FC3GJ315S032 100- 7.14 Jf2.27e3 l:':\ 7439 %- 7,00 8.00 PFBS-2 FC3G_015S032 100n %- 3 .6 6 e 2 l 6-46 1437 I 3.66e2 : 1437 1 4.74 5.54 0^ 5.00 6.00 7.00 PFOSA FC3G 015S032 P V by LE H 15 -Jan -13 , S V by A P 1 6 -Jan -13 . N O T E : O P R Conen. & LO D units = ng/m L, S am p le and Lab B LK Conen. & LO D units = ng/L. Page 129 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnstJ3\PFC.PRO\FC\FC3G_015-B.qid Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-10,R, Name: FC3GJJ15S032 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 04:10:54 oo L..I 13C2-PFOA FC3G 015S032 7.14 J T 2.28e5 864524 %" 0 - 7 ......... -W..-___ tt min 7.00 8.00 13C4-PFOA FC3G 015S032 100- 7.09 A 8 .4 1e4 / \321633 %~ 0- . J . V ...... . min 7.00 8.00 13C4-PFOS-1 FC3G 015S032 100- 8-29 f 1.15e4 44849 i ' o - ............. min 8.00 10.00 12.00 13C4-PFOS-2 FC3G 0T5S032 100- 8.29 9.79e3 39133 %- 0- V min 8.00 10.00 12.00 1802-PFHxS-1 FC3G 015S032 1 0 0 n 7.33 1.03e4~l : 41733 h i % 0/ 7.00 v . min 8.00 18G2-PFHXS-2 FC3G 015S032 100- 7.33 7.25e3~l 29186 j f %- / 0 7.00 8.00 13C4-PFBA FC3G 015S032 100- 5.24 f 2.55e4 1 102603 %-: I4.03 0" 5.00 6.00 7.00 13C2-PFHxA FC3G 015S032 100- 6.36 5.70e4 264391 %- i ; \ o: ----------------: min 5.00 6.00 7.00 13C5-PFNA FC3G 015S032 100- 7.49 7.80e4 / 312972 . %: 0-j 7.00 8.00 13C2-PFDA FC3G__015S032 100- 7.99 8.44 e4 312649 %- 13C2-PFDoA FC3G_015S032 100 9.12 1.31e5 566555 13C2-PFOUEA FC3G 015S032 8.00 10.00 12.00 8.00 10.00 12.00 P V by LEH 15 -Jan -13 , S V by A P 16 -Jan -13 . N O T E : O P R Conen. & LO D units = ng/m L, S am ple and Lab B LK Conen. & LO D units = ng/L. Page 130 of 307 www.axysanalytical.com Quantify Sam ple R eport M assL ynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-10.R, Name: FC3G_015S032 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 04:10:54 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 1 0 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFOA 2 1 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHxS-1 24 18Q2-PFHxS~2 25 13C4-PFBA 26 13C2-PFHXA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.26 26 262.98 >218.... 5.99 26 312.97> 268.98 6.36 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.14 27 462.96 >418.... 7.49 28 512.96 >468.... 7.99 28 562.96 > 518.... 8.64 29 612.95 >568.... 9.12 23 298.94> 79.96 6.51 24 298.94 >98.95 6.46 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 2 1 498.93> 79.96 8 . 1 1 2 2 498.93> 98.96 8.29 2 1 498 > 78 9.96 20 414.97 >369.... 7.14 416.97 > 371.9 7.09 30 503 > 79.96 8.29 30 503 > 98.96 8.29 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.24 30 314.97>269.98 6.36 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 >569.... 9.12 458.98 >393.98 7.38 Area 6630 1775 5029 3600 9264 2266 1580 1613 259 290 827 366 380 161 148 1933 1032 39 227953 84129 11516 9792 10312 7248 25523 57025 78040 84412 131125 29314 IS Area 25523 57025 57025 57025 227953 227953 78040 84412 84412 131125 10312 7248 10312 7248 10312 11516 9792 11516 84129 29314 29314 29314 29314 29314 29314 29314 29314 29314 ng/L 6.144 1.096 1.561 1.292 2.647 2.895 0.137 0.991 1.216 0.672 0.832 5.104 .3,889 0.195 76.422 27.862 36.155 38.693 39.171 37.174 19.752 22.313 27.133 23.328 23.145 23.734 LOD 0.3035 0.0538 0.2051 0.1276 0.3897 0.6282 0.0647 0.4844 0.1638 0.0356 0.0656 0.1543 0.0197 %Rec 95.3 104.2 90.1 96.5 97.7 92.7 73.9 83.4 101.5 87.2 8 6 .6 106.5 S/N 45 9 35 25 84 0 18 11 6 3 33 22 45 9 18 26 30 5 0 0 878 316 201 1840 130 502 2432 1047 516 3608 Noise 544 918 669 527 401 0 379 405 194 505 136 66 32 65 35 163 84 57 0 0 51 124 208 16 787 526 129 299 1099 31 Height 24363 8584 23492 13411 33791 7439 6647 4522 1194 1641 4555 1437 1448 556 645 4282 2493 266 864524 321633 44849 39133 41733 29186 102603 264391 312972 312649 566555 110562 IS Height Sample size 102603 0.4488 264391 0.4488 264391 0.4488 264391 864524 0.4488 0.4488 864524 0.4488 312972 0.4488 312649 0.4488 312649 0.4488 566555 0.4488 41733 29186 0.4488 0.4488 41733 29186 0.4488 0.4488 41733 0.4488 44849 0.4488 39133 0.4488 44849 0.4488 321633 0.4488 0.4488 110562 0.4488 110562 0.4488 110562 0.4488 110562 0.4488 110562 0.4488 110562 0.4488 110562 0.4488 110562 110562 0.4488 0.4488 0.4488 Factor2 Detection... 1 . 0 MM 1 . 0 MM 1 .0 bb 1 . 0 MM 1 .0 bb 1 . 0 bb 1 . 0 bb 1 . 0 MMi 1 . 0 MMI 1 . 0 MMi 1 . 0 MM 1 . 0 MM 1.0 bbl 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 .0 bb 1 . 0 bb 1 .0 bb 1 .0 bb 1 .0 bb 1 .0 bb 1 . 0 bb 1 . 0 MM 1 .0 bb 1 . 0 bb 1 .0 bb 1 .0 bb P V by L E H 15 -Jan -13 , S V by A P 1 6 -Jan -13 . N O T E : O P R C onen. & LO D units = ng/m L, S am p le and Lab BLK Conen. & LO D units = ng/L. Page 131 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: L18989-11,, Name: FC2G_455S021 D escription: 1,WG42273,15/4000uL Date: 18-Dec-2012 Time: 23:49:24 PFBA FC2G_455S021 100- 5.14 A 5.28e3 / ; 16846 : 4.03 : ' 5 6 7 6.60 ': v _ .......................... min 5.00 6.00 7.00 PFNA FC2G 455S021 PFPeA FC2G 455S021 100- 5.98 1.60e3 7734 5.54 %~ 1 6.56 CO < q/ \, 0 - TriT~jn11jm min 5.00 6.00 7.00 PFDA FC2G_455S021 100- 7.99 i~ 1.05e3 2885 %- 10.59 11-66 PFHxA FC2G__455S021 100- 6.36 4.08e3 1 18266 %" 5 1 ^ 5 98 05.00 6.00 7.00 PFUnA FC2G 455S021 100-1 - 7.81 / 11.96 \ .` jl j 11.63 ji j %- i f f 0 10.92 A / V PFHpA FC2G_455S021 1 0 0 "3 6.69 3.74e3 A 14307 % - 5.54^5.82 I 4.42 0 5.00 6.00 7.00 PFDoA FC2G 455S021 1 0 0 - 7.53 12.11 11.16 N %M PFHxS-1 FC2G 455S021 0J .|................... 8.00 10.00 12.00 0 T -I TT m jn 8.00 10.00 12.00 0 8.00 10.00 12.00 PFHxS-2 FC2G 455S021 100- 7.30 A 8.94e1 j \ 432 6 .6 8 , 0- 7.00 A -t min 8.00 PFHxS-3 FC2G_455S021 100] 7.25 %- 0J 7.00 . 8.14 8.00 PFOS-1 FC2G 455S021 PFOA-1 FC2G 455S021 PFBS-1 FC2G_455S021 100- 6.51 _ 7.77e2 3351 %- 5.09 5.63 : 5.00 6.00 7.00 PFOS-2 FC2G__455S021 100- 8.17 |l 1.11e3 H 2934 i 9.84 10.68^2.02 8.00 10.00 12.00 PFOA-2 FC2G 455S021 100- 7.06 /T2.53e3 / \ 9445 %~ 0- / '. 7.00 8.00 PFBS-2 FC2G_455S021 100- 6-43 1375 Sr-3.42e2 1375 4.51 5.78 (S k 5.00 6.00 7.00 PFOSA FC2G_455S021 100- 9.90 7.33e1 394 %- 9-4 5 j 10.56 m / pvs:-78 min 8.00 10.00 12.00 PV by L H E 0 8 -J a n -1 3 , S V by A P 10-Jan -13. N O T E : O P R Conen. & LO D units = ng/m L, S am p le and Lab BLK C onen. & LO D units = ng/L. Page 132 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: L18989-11,, Name: FC2G_455S021 D escription: 1,WG42273,15/4000uL Date: 18-Dec-2012 Time: 23:49:24 13C2-PFOA FC2G_455S021 100- _ 7.11 | 1.40e5 1 545473 %~ 13C4-PFOA FC2G_455S021 100- _ 7.06 | 5.33(24 216320 %" 7.00 8.00 0- ..... 7.00 8.00 13C4-PFBA FC2G,,455S021 100 ?T 1 96e4 ] \ 63771 13C2PFHxA FC2G 455S021 4.08 f'4 5.00 6.00 7.00 13C4-PFOS-1 FC2G 455S021 100-n 8.23 f 8.69e3 - 31909 %: i 0- 8.00 10.00 min 12.00 13C4-PFOS-2 FC2G 455S021 8.23 1 0 0 n f 5.96e3 1 21540 1 %- 1 0 'Ifc j . -rrrrrr min 8.00 10.00 12.00 1802-PFHxS-1 FC2G_455S021 1 0 0 -q - 7.30 j \ 7.44e3 i \ 28034 %' 7.00 8.00. 13C5-PFNA FC2G 455S021 13C2-PFDA FC2G_455S021 100- 7.93 I " 6.04e4 1 220834 iI 0 L- .:.: . _ 8.00 10.00 12.00 13C2-PFDoA FC2G_455S021 100n 9.03 r 8.39e4 I 379533 %L 0A 8.00 10.00 12.00 1802-PFHxS-2 FC2G_455S021 100- 7.25 ;U .93e3 / 17283 %- /\ 7.00 8.00 13C2-PFOUEA FC2G 455S021 P V by LFIE 0 8 -J a n -1 3 , S V by A P 10 -Jan -13. N O T E : O P R C onen. & LO D units = ng/m L, S am p le and Lab BLK Conen. & LO D units = ng/L. Page 133 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: L18989-11,, Name: FC2G_455S021 D escription: 1,WG42273,15/4000uL Date: 18-Dec-2012 Time: 23:49:24 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 1 0 PFDoA 1 1 PFBS-1 1 2 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFOA 2 1 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHxS-1 24 1802~PFHxS~2 25 13C4-PFBA 26 13C2~PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.14 26 262.98 > 218.... 5.98 26 312.97> 268.98 6.36 26 362.97> 318.98 6.69 19 412.97> 368.98 7.06 19 412.97> 168.80 7.06 27 462.96 >418.... 7.49 28 512.96 >468.... 7.99 28 562.96 >518.... 29 612.95 > 568.... 9.09 23 298.94> 79.96 6.51 24 298.94 > 98.95 6.43 23 398.84 > 79.96 7.25 24 398.84> 98.96 7.30 23 398.84> 118.70 2 1 498.93> 79.96 8.05 2 2 498.93> 98.96 8.17 2 1 498 > 78 9.90 2 0 414.97 > 369.... 7.11 416.97 > 371.9 7.06 30 503 > 79.96 8.23 30 503 > 98.96 8.23 30 402.9>84 7.30 30 402.9>103 7.25 30 216.99>171.98 5.13 30 314.97>269.98 6.36 30 467.96>422,97 7.46 30 514.96 >469.96 7.93 30 614.95 >569.... 9.03 458.98 >393.98 7.39 Area 5281 1604 4084 3741 7921 2532 1410 1046 257 777 342 317 89 1979 1105 73 140272 53303 8688 5963 7443 4935 19614 40492 55256 60446 83853 22131 IS Area 19614 40492 40492 40492 140272 140272 55256 60446 60446 83853 7443 4935 7443 4935 7443 8688 5963 8688 53303 22131 22131 22131 22131 22131 22131 22131 22131 22131 ng/L 6.0V1.590 2.095 2.105 3.622 4.678 0.612 2.139 2.812 0.590 1.123 7.446 6.935 0.484 62.456 25.731 31.738 29.407 31.313 29.141 16.763 16.075 22.104 19.973 18.860 22.705 LOD 0.2806 0.5156 0.0466 0.6832 0.0911 0.1380 0.1423 0.9525 0.6038 0.2849 0.4032 0.2043 0.9204 0.0990 0.6355 0.1063 0.1753 0.1940 0.0288 %Rec S/N 39 13 33 10 158 458 14 9 3 21 16 17 18 86.5 106.9 87.9 81.4 86.7 80.7 69.6 6 6 .8 91.8 82.9 78.3 113.1 25 31 11 0 0 444 90 928 0 67 584 0 324 343 2363 Noise 437 583 549 1454 202 21 394 338 294 278 156 87 78 24 60 206 93 37 0 0 72 240 30 0 955 344 0 681 1107 36 Height 16846 7734 18266 14307 31761 9445 5487 2885 810 3351 1375 1315 432 5201 2934 394 545473 216320 31909 21540 28034 17283 63771 200743 238690 220834 379533 85232 IS Height 63771 200743 200743 200743 545473 545473 238690 220834 220834 379533 28034 17283 28034 17283 28034 31909 21540 31909 216320 85232 85232 85232 85232 85232 85232 85232 85232 85232 Sample size 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 0.4983 Factor2 Detection... 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 MM 1 . 0 MM! 1 .0 1 . 0 MM! 1 . 0 bb 1 . 0 bb 1 . 0 MM 1 . 0 MM 1 .0 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb P V by LH E 0 8 -J a n -1 3 , S V by A P 10 -Jan -13 . N O T E : O P R C on en. & L O D units = ng/m L, S am p le and Lab B LK Conen. & LO D units = ng/L. Page 134 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: WG42273-103,,DUP Name: FC2G_455S022 D escription: 1,WG42273,15/4000uL Date: 19-Dec-2012 Time: 00:07:06 PFBA FC2G_455S022 ico- | _4.c83e3 15838 4.08 \ 6.04 ... min 5.00 6.00 7.00 PFPeA FC2G 455S022 1 0 0 -| 2.07e31 10647 ,| % - 5.69 j i 4.03 ij 6.47 ,, 0- V .l|.......TrmTTITTTfTn min 5.00 6.00 7.00 PFNA FC2G 455S022 PFDA FC2G 455S022 PFHxS-1 FC2G 455S022 PFHxS-2 FC2G_455S022 100- 7.25 /T 1.16e2 /436 %- 7.00 8.00 PFHxA FC2G 455S022 100" %-; 4 .3 7 e 3 l 19377 i :i 5 33 s eo . 0 ~ ...... - "l-- '* -. 5.00 6.00 7.00 . PFUnA FC2G__455S022 100- 12.41 12.14^ . 11-13 T >/ liWl m u 'vV' 8.00 10.00 12.00 PFHxS-3 FC2G__455S022 100- 7.25 7.46 / :A J 7.00 \7 .7 8 8.00 PFHpA FC2G 455S022 10 0 -, 3.31 e3 h 12705 ; %- 5.37 - 4.79 - \ 0 ~ min 5.00 6.00 7.00 PFOA-1 FC2G 455S022 100- j'i 8.31e3 / 129822 %-- ... j .. 0- 7.00 . . min 8.00 PFOA-2 FC2G_455S022 ioo-q 7.06 : 2.34e3 8941 %- 07.00 i.00 PFDoA FC2G_455S022 100- 9.03 3.43e2 1955 11.99 8.05 1 9-93 Jl \a \AJM W > // 0-Vr 8.00 10.00 2.00 PFBS-1 FC2G_455S022 100- 6-43 6.43 7.30e2~|" 7.30e2 3007 1 30Q7 5.83 5.59. ft m 5.05. 5.00 6.00 7.00 PFBS-2 FC2G_455S022 100- 6.47 3 .3 0 e 2 l 1667 %- 4 - 5 5.39 5.00 6.00 7.00 PFOS-1 FC2G_455S022 100- _ 7*99 l 2.14e3 I 5660 %- \ 1116 12.20 8.00 10.00 12.00 PFOS-2 FC2G_455S022 100- 8.17 18e2 : 2381 % 12.38 0-" 9.63 1J0'.-8A0jJ 8.00 10.00 12.00 PFOSA FC2G_455S022 100--t 9.93 8.65e1 j' 392 I %9.60 I 11.22 12.02 C 8.00 10.00 12.00 P V by LH E 0 8 -J a n -1 3 , S V by A P 10-Jan -13 . N O T E : O P R Conen. & LO D units = ng/m L, S am p le and Lab B LK C onen. & LO D units = ng/L. m Page 135 of 307 www.axysanalytical.com Quantify Sample R eport M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: WG42273-103,,D UP Name: FC2G_455S022 D escription: 1,WG42273,15/4000uL Date: 19-Dec-2012 Time: 00:07:06 13C2-PFOA FC2G__455S022 100- 7.06 f 1.41e5 1558029 %- 13C4-PFOA FC2G_455S022 100- _ 7.06 i 4.91 e4 1;197609 0/O/ -- 13C4-PFOS-1 FC2G,,455S022 100- 8.17 f 8.17e3 P 30698 % 7.00 8.00 13C4-PFBA FC2G,,455S022 100- 5.14 r I.89e4 M 66436 %- 14.14 ;h ...L i 5.00 6.00 7.00 7.00 8.00 8.00 10.00 12.00 13C2-PFHxA F'C2G 455S022 100q 6.34 4.07e4 i 186784 n % !' i - 0 - m min 5.00 6.00 7.00 13C5-PFNA FC2G__455S022 7.46 1 0 0 n 5.67e4 A 239422 ; \ %: o: 7.00 8.00 13C4-PFOS-2 FC2G_455S022 100- _ 8.17 7.11e3 I 28131 %- 0 V.^n 8.00 10.00 12.00 13C2-PFDA FC2G_455S 022 100- _ 7.93 6.36e4 241225 %- 8.00 10.00 12.00 1802-PFHxS-1 FC2G__455S022 100- 7.25 |"7.49e3 k 31465 %- 7.00 8.00 13C2-PFDoA FC2G_455S022 1001 9.03 r 9.08e4 %- HP '399904 ll ;; 8.00 10.00 12.00 1802-PFHxS-2 FC2G_455S022 100- 7.25 ,|~5.10e3 M 21761 %- 7.00 8.00 13C2-PFOUEA FC2G 455S022 P V by LH E 0 8 -J a n -1 3 , S V by A P 10 -Jan -13 . N O T E : O P R C onen. & LO D units = ng/m L, S am p le and Lab BLK C onen. & LO D units = ng/L. Page 136 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: WG42273-103,,DUP Name: FC2G_455S022 D escription: 1,WG42273,15/4000uL Date: 19-Dec-2012 Time: 00:07:06 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 , PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 1 0 PFDoA 1 1 PFBS-1 1 2 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFA 2 1 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHXS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOEA IS# Trace RT 25 212.99>168.98 5.13 26 262.98 > 218.... 5.95 26 312.97> 268.98 6.34 26 362.97> 318.98 6.69 19 . 412.97> 368.98 7.06 19 412.97> 168.80 7.06 27 462.96 > 418.... 7.46 28 512.96 > 468.... 7.93 28 562.96 >518.... 29 612.95 >568.... 9.03 23 298.94> 79.96 6.43 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.25 24 398.84> 98.96 7.25 23 398.84> 118.70 2 1 498.93> 79.96 7.99 2 2 498.93> 98:96 8.17 2 1 498 > 78 9.93 2 0 414.97 > 369.... 7.06 416.97 >371.9 7.06 30 503 > 79.96 8.17 30 503 > 98.96 8.17 30 402.9>84 7.25 30 402.9>103 7.25 30 216.99>171.98 5.14 30 314.97>269.98 6.34 30 467.96>422.97 7.46 30 514.96 >469.96 7.93 30 614.95 > 569.... 9.03 458.98 >393.98 7.39 Area 4833 2070 4367 3305 8311 2336 1245 1564 343 730 330 220 116 2142 918 87 141479 49058 8172 7113 7488 5101 18859 40733 56715 63565 90751 20501 IS Area 18859 40733 40733 40733 141479 141479 56715 63565 63565 90751 7488 5101 7488 5101 7488 8172 7113 8172 49058 20501 20501 20501 20501 20501 20501 20501 20501 20501 ng/L 5.714 1.98' 2.235 1.879 3.774 4.285 0.529 1.952 2.653 0.188 1.294 8.623 4.582 0.519 68.438 23.680 32.223 37.865 34.002 32.515 17.398 17.455 24.489 22.672 22.032 21.031 LOD 0.6330 0.6435 0.0911 0.5750 0.0621 0.1324 0.1268 0.7651 0.7086 0.4079 0.2903 0.3195 0.7249 0.1399 0.9073 0.1417 0.0796 0.2322 %Rec S/N 20 14 30 12 146 0 12 11 5 17 24 5 9 94.7 98.3 89.2 104.8 94.1 90.0 72.3 72.5 101.7 94.2 91.5 104.8 58 31 10 0 0 313 352 148 745 51 424 0 812 314 0 Noise 794 772 636 1018 204 0 376 429 252 358 178 70 142 50 34 98 77 39 0 . 0. 98 80 213 29 1309 440 0 297 1273 0 Height 15838 10647 19377 12705 29822 8941 4619 4880 1955 3007 1667 741 436 5660 2381 392 558029 197609 30698 28131 31465 21761 66436 186784 239422 241225 399904 81878 IS Height Sample size 66436 0.4984 186784 0.4984 186784 0.4984 186784 0.4984 558029 0.4984 558029 0.4984 239422 0.4984 241225 0.4984 241225 0.4984 399904 0.4984 31465 0.4984 21761 0.4984 31465 0.4984 21761 0.4984 31465 0.4984 30698 0.4984 28131. 0.4984 30698 0.4984 197609 0.4984 0.4984 81878 0.4984 81878 0.4984 81878 0.4984 81878 81878 81878 0.4984 0.4984 0.4984 81878 0.4984 81878 0.4984 81878 0.4984 0.4984 Factor2 Detection... 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MMi 1 .0 1 . 0 MMi 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 MM 1 .0 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb P V by L H E 0 8 -J a n -1 3 , S V by A P 10-Jan -13 . N O T E : O P R Conen. & L O D units = ng/m L, S am p le and Lab BLK Conen. & LO D units = ng/L. Page 137 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G__015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-12,R, Name: FC3G_015S033 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 04:27:55 PFBA FC3G 015S033 100 5.21 1.03&4 PFPeA FC3G__015S033 100-, 5.95 5.53e3~| 18631 I %~ 5.69 . , 6.59 4.18 V PFHxA FC3G_015S033 100- 6.34 1.23e4 54190 % 4.07 PFHpA FC3G_015S033 100- 6.73 9.97e3 36552 M iI : i. 4.135 795;95 / | 5.00 6.00 7.00 5.00 6.00 7.00 5.00 6.00 7.00 5.00 6.00 7.00 PFNA FC3GJH5S033 100- 7.49 2.37e3 8679 %-_16.63 7.00 8.00 PFDA FC3G_015S033 100- 7.99 f 2.03e3 I 6026 %- 8.64 11.69 tjtt min 8.00 10.00 12.00 PFUnA FC3G_015S033 n rr A 9.66 12,23 8.00 10.00 12.00 PFDoA FC3G_015S033 100- 9.18 j3-20e2 1337 12.29 I %- 7.70 110.02 ; . / . ..v 8.00 10.00 12.00 PFHxS-1 FC3G 015S033 PFHxS-2 FC3G 015S033 100- 7.29 !\ 4.84e2 \ 1552 %6.71 / o7.00 \7 .9 0 8.00 min oo PFHxS-3 FC3G 015S033 7.33 3.00e2?: 943 / % 6 ,86/ o- 7.00 ~ --- min 8.00 PFOS-1 FC3G 015S033 8.29 1 0 0 -j I f 6,23e3 M 12196 %- 10;4 7 ___ 0 8.00 10.00 12.00 PFOA-1 FC3G_015S033 100- 7.09 A 3}.006e4 116 410 i\ %- 7.00 8.00 PFBS-1 FC3G__015S033 100- 6.47 6.75e3 30376 %- 0~~Wfrfrr 5.00 6.00 7.00 PFOS-2 FC3G 015S033 PFOA-2 FC3G_015S033 . 100- _ 7.09 . 8.89e3 \ 32775 %- 7.00 8.00 PFBS-2 FC3G 015S033 100- 6.47 3.61 e3~| 16645 | %- 1 m o- 5.00 6.00 7.00 PFOSA FC3G_015S033 mn 1 0 -0 2 100^ 2.59e2~l 1130 g I %H | -7.53 I 10.47 / 11 5 4 8.00 10.00 12.00 P V by LE H 1 5 -Jan -13 , S V by A P 1 6 -J a n -1 3 . N O T E : O P R Conen. & LO D units = ng/m L, S am p le and Lab BLK C onen. & L O D units = ng/L. Page 138 of 307 www.axysanalytical.com Quantify Sam ple Report MassLynx4.1 SCN627 Dataset: G :\M as s ly nx \in s t_ G \P F C .P R O \F C \F C 3G .. 015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-12.R, Name: FC3G_015S033 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 04:27:55 13C2-PFOA FC3G__015S033 100" 7n "2.31 e5 14 9 2 6 4 8 3 . . min 7.00 8.00 13C4-PFOA FC3G 015S033 100- JT 8.33e4 { 1339141 %-- o7.00 -`tt min 8.00 13C4-PFOS-1 FC3G 015S033 1 0 ~ r 1.25e4 | 47066 %- 0- illk j.. min 8.00 10.00 12.00 13C4-PFOS-2 FC3G 015S033 1001 r 9.58e3 | 36142 H 0 min 8.00 10.00 12,00. 1802-PFHxS-1 FC3G 015S033 100- 1.1.7e4"| 46323, %~ /\ 0 -T min 7.00 8.00 1802-PFHxS-2 FC3G 015S033 1 0 0 - 8.46e3 A 32872/ %-- 0 8.00 13C4-PFBA FC3G_015S033 100- 5.21 f 2.66e4 I 103488 13C2-PFHxA FC3G_015S033 100- 6.34 5.49e4 l 245900 h 13C5-PFNA FC3GJJ15S033 100- 7.49 8.34e4 i\ 336754 ! \ %- 13C2-PFDA FC3G_015S033 100- . 7.99 f 9.09e4 i 331791 %- 13C2-PFDoA FC3G_015S033 100n 9.18 r 11.425 615194 13C2-PFOUEA FC3G 015S033 5.00 6.00 7.00 5.00 6.00 7.00 7.00 8.00 8.00 10.00 12.00 IL.. 8.00 10.00 12.00 P V by LE H 1 5 -J a n -1 3 , S V by A P 16-Jan~13. N O T E : O P R C onen. & LO D units = ng/m L, S am p le and Lab B L K C o n c n . & LO D units = ng/L. Page 139 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3GJ315-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-12.R, Name: FC3G_015S033 D escription: 1,WG42428,15/4000uL Date: 15^Jan-2013 Time: 04:27:55 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 1 1 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFOA 2 1 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.21 26 262.98 >218.... 5.95 26 312.97> 268.98 6.34 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.49 28 512.96 >468..,. 7.99 28 562.96 > 518-.. 29 612.95 > 568-- 9.18 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.29 23 398.84> 118.70 7.33 21 498.93> 79.96 8.29 22 498.93> 98.96 8.29 21 498 > 78 10.02 20 414.97 > 3 6 9 -- 7.09 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.29 30 402.9>84 7.33 30 402.9>103 7,33 30 216.99>171.98 5.21 30 314.97>269,98 6.34 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.18 458.98 >393.98 7.38 Area 10325 5529 12283 9966 30567 8895 2370 2034 320 6751 3607 887 484 300 6234 4240 259 231143 83326 12514 9582 11702 8463 26570 54935 83356 90903 141892 29222 IS Area 26570 54935 54935 54935 231143 231143 83356 90903 90903 141892 11702 8463 11702 8463 11702 12514 9582 12514 83326 29222 29222 29222 29222 29222 29222 29222 29222 29222 ng/L 9.205 3.679 4.646 3.935 9.727 11.998 0.354 14.991 15.122 1.117 2.026 1.979 17.256 17.712 0.606 76.584 27.013 38.579 37.178 43.647 42.623 20.190 21.106 28.457 24.668 24.592 23.160 LOD 0.2189 0.0344 0.2238 0.1713 0.1713 0.4129 0.0538 0.4545 0.3312 0.1535 0.0223 0.1431 0.0801 %Rec S/N 118 27 71 52 651 0 15 21 97.5 103.2 98.3 94.7 111.2 108.6 77.1 80.6 108.7 94.2 94.0 106.2 6 172 253 121 13 33 83 107 28 0 0 674 270 2553 290 189 489 4095 500 1058 0 Noise 345 683 762 707 179 0 569 286 421 226 176 66 20 122 28 146 120 40 0 0 70 134 18 113 548 502 82 663 581 0 Height 40650 18631 54190 36552 116410 32775 8679 6026 1337 30376 16645 2459 1552 943 12196 12803 1130 926483 339141 47066 36142 46323 32872 103488 245900 336754 331791 615194 110229 IS Height Sample size 103488 0.4585 245900 0.4585 245900 0.4585 245900 0.4585 926483 0.4585 926483 0.4585 336754 0.4585 331791 0.4585 331791 0.4585 615194 0.4585 46323 0.4585 32872 0.4585 46323 0.4585 32872 0.4585 46323 0.4585 47066 0.4585 36142 0.4585 47066 0-4585 339141 0.4585 0.4585 110229 0.4585 110229 0.4585 110229 0.4585 110229 0.4585 110229 0.4585 110229 0.4585 110229 0.4585 110229 0.4585 110229 0.4585 0.4585 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1 . 0 MM 1.0 MM 1.0 MM) 1.0 1.0 bbl 1.0 MM 1.0 MM 1 . 0 bb 1.0 MM 1 . 0 bb 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1 . 0 bb 1 . 0 bb 1.0 MM 1.0 bb 1.0 MM 1 . 0 bb 1,0 bb 1.0 bb 1.0 bb P V by LEH 15-Jan-13, S V by A P 16-Jan-13. N O TE : O P R Conen. & LO D units = ng/m L, S am ple and Lab BLK Conen. & LO D units = ng/L. Page 140 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\InstJ3\PFC.PRO\FC\FC3G_015-B.qid Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-13,R, Name: FC3G_015S034 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 04:44:56 PFBA FC3G 015S034 100- 5.21 f 8.43e3 jj 38045 %- II 4.09 l 0 - Wr^iiiipTrriTiTTr-n--Trrrrr mi.n 5.00 6.00 7.00 PFPeA FC3G_015S034 5.95 100 3.61 e 3 l 14779 I% 5.41 M 0 5.00 6.00 7.00 PFHxA FC3G_015S034 100- 6.33 8.98e3 ' 42836 %- 5.10 5.84 5.00 6.00 7,00 PFHpA FC3G 015S034 PFNA FC3G__015S034 PFDA FC3G 015S034 100- 7.99 r 1.80e3 6281 % ;S 12.08 i , 9-90 '.. 0 - L1.,.......v..`.'.V..... - -----------'---- 8.00 10.00 12.00 PFUnA FC3G 015S034 1 0 0 -n 8.49 1 2 :08 I 1/F 3.12e2 I 12.35 1413 11-84 ,1 I \f % J \ 1 . ,'i r.'VW h WW 0^ 8.00 10.00 12.00 PFDoA FC3G 015S034 100- 9 12 -5 .6 3 e 2 . 1519 8.17 %- I 2 ;2 6 0 - ----- --- ----------- min 8.00 10.00 12.00 PFHxS-1 FC3G_015S034 100 / 6.'83u2 \ 2262 16.66 } 7.00 \ \ 8.02 8.00 min PFHxS-2 FC3G 015S034 100- JT4.52e2 1761 %- 07.00 rF min 8.00 PFHxS-3 FC3G 015S034 100^ T2.94e2 l 903 %- 6.63 / '/ 0- " `\y -- .... . -v--' \ mm PFOS-1 FC3G 015S034 100%- f4 .6 0 e 3 | 10689 4 U 10 - - T-rrTTTTTr r nnn PFOA-1 FC3G 015S034 100- 7.05 H2.22e4 \ 83570 %-- PFOA-2 FC3G 015S034 07.00 8,00 PFBS-1 FC3G_015S034 100-3 6.47 5.00 6.00 7.00 PFBS-2 FC3G 015S034 100- 6.44 2 .1 9 e 3 l 10268 1 1 %- i Q 0- ........ ... Iff 5.00 6.00 7.00 PFOS-2 FC3G 015S034 100 r 2.36e3 1r.: 5575 %: H i 0 - -----------I -- 11.39 12.05 -- --v m jn PFOSA FC3G_015S034 9.99 1.71e2~| 635 I oO %7.93 o: _ 11.90 min P V by LE H 15-Jan~13, S V by A P 16 -Jan -13 . N O T E : O P R Conen. & LO D units = ng/m L, S am p le and Lab BLK Conen. & L O D units = ng/L. Page 141 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-13,R, Name: FC3G_015S034 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 04:44:56 13C2-PFOA FC3G_015S034 100- ^ 7.09 || 2.05e5 | 829906 %' 7.00 8.00 13C4-PFOA FC3G 015S034 100- 7.09 IT 7.6 3e4 M 308093 %- J o- - w . ... tt min 7.00 8.00 13C4-PFOS-1 FC3G 015S034 100- 8.29 i 1.17e4 1 44365 8 % - 11 fj M o8.00 10.00 min 12.00 13C4-PFOS-2 FC3G_015S034 100n 8.29 8.52e3 33364 %- 0-' 8.00 10.00 12.00 13C4-PFBA FC3G__015S034 5.19 100^ j~~2.16e4 i 102104 13C2-PFHxA FC3G_015S034 100-j 6.31 4.86e4 223037 %- 13C5-PFNA FC3G 015S034 13C2-PFDA FC3G_015S034 m1UnU"i |r~8,, .72-69e94 1 292369 %- i 6.00 6.00 7.00 A. 5.00 6 .0 0 7.00 8.00 10.00 12.00 1802-P F H xS -l FC3G_015S034 100- 7.29 JT9.80e3 I I 41216 %- 1802-PFHxS-2 FC3G_015S034 100- 7.29 f 7,35e3 1 30799 %` 7.00 8.00 13C2-PFDoA FC3G 015S034 100- 9.12 f 1-14e5 1 474258 s%~ 8.00 . -tt min 10.00 12.00 7.00 8.00 13C2-PFOUEA FC3G 015S034 100- 7.33 2.78e4 102115 %- / o- 7.00 .... 8.00 P V by LE H 15 -Jan -13 , S V by A P 1 6 -Jan -13 . N O T E : O P R C o n c n . & L O D units = ng/m L, S am p le and Lab BLK C onen. & LO D units = ng/L. Page 142 of 307 www.axysanalytical.com Quantify Sam ple R eport M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-13,R, Name: FC3G_015S034 D escription: 1,WG42428,15/4000uL Date: 15Jan-2013 Time: 04:44:56 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 1 0 PFDoA 11 PFBS-1 1 2 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 - 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFOA 2 1 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHxS-l 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.21 26 262.98 > 218.... 5.95 26 3 12.97> 268.98 6.33 26 362.97> 318.98 6 . 6 8 19 412.97> 368.98 7.05 19 412.97> 168.80 7.09 27 462.96 >418.... 7.46 28 512.96 >468.... 7.99 28 562.96 > 518.... 8.49 29 612.95 >568.... 9.12 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.44 23 398.84 > 79.96 7.29 24 398.84> 98.96 7.29 23 398.84> 118.70 7.29 2 1 498.93> 79.96 8.17 2 2 498-93> 98.96 8.23 2 1 498 > 78 9.99 2 0 414.97 > 369.... 7.09 416.97 >371.9 7.09 30 503 > 79.96 8.29 30 503 > 98.96 8.29 30 402.9>84 7.29 30 402.9>103 7.29 30 216.99>171.98 5.19 30 314.97>269.98 6.31 30 467.96>422.97 7.46 30 514.96 >469.96 7.99 30 614.95 >569.... 9.12 458.98 >393.98 7.33 Area 8433 3613 8979 7298 22220 5781 2192 1798 312 563 4559 2186 683 452 294 4604 2363 171 205116 76278 11715 8516 9796 7354 21611 48587 72891 82467 114189 27837 IS Area 21611 48587 48587 48587 205116 205116 72891 82467 82467 114189 9796 7354 9796 7354 9796 11715 8516 11715 76278 27837 27837 27837 27837 27837 27837 27837 27837 27837 ng/L 8891 2.591 3.608 3.106 7.562 8.358 0.383 11.388 9.942 0.894 2.108 2.332 12.840 10.485 0.441 71.387 23.778 36.455 33.353 36.883 37.384 16.577 18.844 25.119 22.590 19.978 21.214 LOD 0.1776 0.1052 0.9408 0 .0 2 1 0 0.1431 0.1307 04145 0.2169 0.0157 1.5459 0.1995 0.0327 0.0776 0.0932 %Rec 94.5 94.5 96.6 88.3 97.7 99.0 65.9 74.9 99.8 89.8 79.4 1 0 1.1 S/N 77 27 98 30 0 304 54 12 3 5 106 91 0 6 10 90 57 26 0 0 864 258 585 8178 41 355 2636 844 728 0 Noise 494 552 438 888 0 69 163 529 404 288 197 113 0 312 91 119 98 24 0 0 51 129 70 4 2464 629 116 346 651 0 Height 38045 14779 42836 26436 83570 . 20971 8762 6281 1413 1519 20804 10268 2262 1761 903 10689 5575 635 829906 308093 44365 33364 41216 30799 102104 223037 306152 292369 474258 102115 IS Height Sample size 102104 0.4768 223037 0.4768 223037 0.4768 223037 0.4768 829906 0.4768 829906 0.4768 306152 0.4768 292369 0.4768 292369 0.4768 474258 0.4768 41216 0.4768 30799 0.4768 41216 0.4768 30799 0.4768 41216 0.4768 44365 0.4768 33364 0.4768 44365 0.4768 308093 0.4768 0.4768 102115 0.4768 102115 0.4768 102115 0.4768 102115 0.4768 102115 0.4768 102115 0.4768 102115 0.4768 102115 0.4768 102115 0.4768 0.4768 Factor2 Detection... 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MM 1 . 0 MMi 1 . 0 MM! 1 . 0 MM! 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 MM 1 . 0 bb 1 . 0 MM 1 . 0 MM 1 . 0 bb 1 . 0 bb . 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 : 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb P V by LE H 15 -Jan -13 , S V by A P 16 -Jan -13 . N O T E : O P R C on en. & LO D units = ng/m L, S am p le and Lab B L K C o n c n . & LO D units = ng/L. Page 143 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-14,R, Name: FC3GJD15S035 D escription: 1,WG42428,15/400OuL Date: 15-Jan-2013 Time: 05:01:49 PFBA FC3G 015S035 1 0 0 -] . 5.21 A 1.03e4 : . 35887 %- 4.07 \ 6 -.1 4 0 " -rrr rrtin 5.00 6.00 7.00 PFPeA FC3G_015S035 5.95 __ 100 4 .1 1e3 16000 % 5.60 6.40 4.11 l V . 0 5.00 6.00 7.00 PFHxA FC3G__015S035 100- 6.36 _ 1.42e4 61393 j %- rill 5.00 6.00 7.00 PFHpA FC3G_015S035 100- 6.73 _ 9.46e3 l 31255 M %- 5.64,6.01 4.03 5.00 6.00 7.00 PFOA-1 FC3G_015S035 100- 7.09 A 3.14e4 M 115666 %- 0-- - ' 7.00 8.00 PFOA-2 FC3G_015S035 7.09 100- I 9.43e3 ^ 35849 %- 7.00 8.00 PFNA FC3G 015S035 PFDA FC3G_015S035 _ 7.93 100n 2.42e3 8454 %p , 9.78 11-5112.23 8.00 10.00 12.00 PFUnA FC3G_015S035 100- 12.41 11.96- n a1j u0..o3o8v^ 1 f | ,1 ,V,rtl! 1i! 0 - W ttt- T7TT min 8.00 10.00 12.00 PFDoA FC3GJJ15S035 100 7.70 1 9.03 2.96e2 11,.87 %c Iri# W mi 0 8.00 10.00 12.00 PFBS-1 FC3G_015S035 100" 5.00 6.00 7.00 PFBS-2 FC3G_015S035 100- 6.47 2.35e3 1 11399 l\ %- 5.00 6.00 7.00 PFHxS-1 FC3G__015S035 100- f9 .7' 1 e 2 | | 2981 ^6 .6 6 ' \ 7.00 p? min 8.00 PFHxS-2 FC3G 015S035 100- A 5.77e2 ; : 2454 %: 6.63 o7.00 ~ ..... -r min 8.00 PFHxS-3 FC3G 015S035 100- %- o - >- .. 7.00 min 8.00 PFOS-1 FC3G 015S035 100 A 6.39e3 i i 14737 % ;j il 01 8.00 10.00 . min 12.00 PFOS-2 FC3G 015S035 1 0 0 - j\ 3.52e3 9355 %~ I i 0 ^ min 8.00 10.00 12.00 PFOSA FC3G_015S035 9.90 1 0 0 -, 1.83e2~| 736 I %-- 8.85 \ r 3 V 6 O- 5 8.00 10.00 12.00 P V by L E H 1 5 -Jan -13 , S V by A P 16-Jan~13. N O T E : O P R C o n c n . & LO D units = mg/mL, S am p le and Lab BLK Conen. & LO D units =.ng/L. m Page 144 of 307 www.axysanalytical.com Quantify Sam ple Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-14.R, Name: FC3G_015S035 D escription: 1,WG42428,15/4000uL Date: 15^Jan-2013 Time: 05:01:49 13C2-PFOA FC3G 015S035 100- 7.09 I " 2.55e5 M 1007994 %- 07.00 . min 8.00 13C4-PFOA FC3G 015S035 100- 7.09 A 8.91e4 S 355915 %" 0 - i J l I k - . . . , -r-T min 7.00 8.00 13C4-PFOS-1 FC3G 015S035 100- 8.23 r 1-33e4 I 50145 %: P P ! 0 - 0 V. 8.00 10.00 min 12.00 13C4-PFOS-2 FC3G 015S035 100- 8.17 l. 1.09&4 y 39814 % ;: 0 ^ _________ min 8.00 10.00 12.00 1802-PFHXS-1 FC3G 015S035 100- 7.29 k 1.11e4 Q 43554 0 i' -- min 7.00 8.00 1802-PFHxS-2 FC3G 015S035 100- 7.29 8.83e3 l \ 35264 %- 07.00 8.00 13C4-PFBA FC3G 015S035 100- 5.21 ! 2.91 e4 ; 114066 %- : \ 4.11 0- i i 5.00 6.00 tt min 7.00 13C2-PFHxA FC3G 015S035 100- 6.36 5.83e41 260598 1 %- O' ;V n-r min 5.00 6.00 7.00 13C5-PFNA FC3G 015S035 7.46 1 0 0 - 9.03e4 h 386666 / %- / 7.00 -r-r min 8.00 13C2-PFDA FC3G 015S035 100- 7.93 F 8.84e4 | 307549 Pi %- 0- .. ........... min 8.00 10.00 12.00 13C2-PFDoA FC3G 015S035 100- 9.03 F 1.39e5 | 626521 %- 08.00 : \ ...... rrr min 10.00 12.00 13C2-PFOUEA FC3G 015S035 100- 7.33 S 3 -2 0 e 4 f i l l 16674 %. nJ / .... : .........- 7.00 8.00 P V by LE H 1 5 -J a n -1 3 , S V by A P 1 6 -J a n -1 3 . N O T E : O P R Conen. & LO D units = ng/m L, S am p le and Lab B LK C onen. & LO D units = ng/L. Page 145 of 307 www.axysanalytical.com Quantify Sample Report M assL ynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: L18989-14,R, Name: FC3G_015S035 D escription: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 05:01:49 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 1 0 PFDoA 11 PFBS-1 1 2 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2~PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.21 26 262.98 > 218.... 5.95 26 312.97> 268.98 6.36 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.46 28 512.96 >468.... 7.93 28 562.96 > 518.... 8.58 29 612.95 > 568.... 9.03 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.29 24 398.84> 98.96 7.29 23 398.84> 118.70 7.29 21 498.93> 79.96 8.17 2 2 498.93> 98.96 8.17 21 498 > 78 9.90 2 0 414.97 > 369..., 7.09 416.97 >371.9 7.09 30 503 > 79.96 8.23 30 503 > 98.96 8.17 30 402,9>84 7.29 30 402.9>103 7.29 30 216.99>171.98 5.21 30 314.97>269.98 6.36 30 467.96>422.97 7.46 30 514.96 >469.96 7.93 30 614.95 >569.... 9.03 458.98 >393.98 7.33 Area 10321 4112 14158 9463 31374 9433 2108 2415 212 296 5140 2350 971 577 404 6390 3523 183 255025 89118 13263 10944 11062 8828 29115 58343 90312 88418 138966 32044 IS Area 29115 58343 58343 58343 255025 255025 90312 88418 88418 138966 11062 8828 11062 8828 11062 13263 10944 13263 89118 32044 32044 32044 32044 32044 32044 32044 32044 32044 ng/L 8.908 2.715 5.418 3.730 9.598 12.272 0.232 12.595 9.792 1.591 2.500 '3.299 17.739 13.571 0.470 84.173 30.781 39.725 41.257 40.088 43.195 21.496 21.779 29.956 23.312 23.401 27.058 LOD 0.2523 0.0300 0.1729 0.4032 0.2396 0.1028 0.3737 0.1652 0.0628 0.0956 0.0716 %Rec 100.6 110.4 95.0 98.6 95.8 103.3 77:1 78.1 107.4 83.6 83.9 116.4 S/N 93 21 135 43 420 0 25 48 4 3 107 180 38 61 76 94 68 20 0 0 307 516 0 1383 186 485 1682 699 1214 0 Noise 388 765 453 725 276 0 385 176 319 378 202 63 78 40 15 157 138 37 0 0 163 77 0 26 614 537 230 440 516 0 Height IS Height Sample size 35887 114066 0.4303 16000 260598 0.4303 61393 260598 0.4303 31255 260598 0.4303 115666 1007994 0.4303 35849 1007994 0.4303 9495 386666 0.4303 8454 307549 0.4303 1276 307549 0.4303 1203 626521 0.4303 21617 43554 0.4303 11399 . 35264 0.4303 2981 43554 0.4303 2454 35264 0.4303 1140 43554 0.4303 14737 50145 0.4303 9355 39814 0.4303 736 50145 0.4303 1007994 355915 0.4303 355915 0.4303 50145 116674 0.4303 39814 116674 0.4303 43554 116674 0.4303 35264 116674 0.4303 114066 116674 0.4303 260598 116674 .0.4303 386666 116674 0.4303 307549 116674 0.4303 626521 116674 0.4303 116674 0.4303 . Factor2 Detection... 1 . 0 MM 1.0 MM 1.0 MM 1 . 0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 MMI 1,0 MMI 1.0 bb! 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 bb 1 . 0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1 . 0 bb P V by LE H 15 -Jan -13 , S V by A P 16 -Jan -13 . N O T E : O P R C onen. & LO D units = ng/m L, S am p le and Lab B LK Conen. & LO D units = ng/L. Page 146 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: WG42273-101,,BLK Name: FC2G_455S010 Description: 1,WG42273,15/4000uL Date: 18-Dec-2012 Time: 20:35:06 PFBA FC2G 455S010 PFPeA FC2G_455S010 100- 6.06 1.71e2 1 %- IIs 48-.-29-50 1*1 6.69 f\ l 7.13 M j j f \y 5.00 6.00 7.00 PFHxA FC2G_455S010 100- 6.37 _ 5.79e2 2698 %-4.03 5.69 116.98 5.00 6.00 7.00 PFHpA FC2G_455S01Q 100- 6.74 _ 3.43e3 I 13572 I %- 06 5.52 5.00 6.00 7.00 PFNA FC2G_455S010 100- 7.49 3.48e2 A 1525 l \ %-36.63 l . j min 7.00 8.00 PFDA FC2G_455S010 100- 7.99 r * 1 .19e3 1 4136 %- 1 11.28 10.26^ \ 12A.35 08.00 10.00 12.00 PFUnA FC2G_455S010 100 7.58 9-87 12.44 11 01 ' 8.88 |\ M hl v \| V 8.00 10.00 -q-rr min 12.00 PFDoA FC2G__455S010 100n . 9-48 12.11 r 0 TT 8.00 a! l/V 10.00 12.00 PFHxS-1 FC2G 455S010 PFHxS-2 FC2G 455S010 PFHxS-3 FC2G_455S010 100-- 7.35 3.61 e l | 195 %- 6.72 * f \7.73 min 0 7,00 8.00 PFOS-1 FC2G 455S010 PFOA-1 FC2G 455S010 PFOA-2 FC2G 455S010 PFBS-1 FC2G 455S010 PFOS-2 FC2G_455S010 100n 8n.23 11.55 12.32 f":3.86e1 I 160 Aiiflw- 90'?v,!' 8.00 10.00 12.00 PFBS-2 FC2G_455S010 100- 5-42 j 6.51 4.06 I A 5.85 |i 6-74 ; M il fj I i-A MAAAA. r 5.00 6.00 7.00 PFOSA FC2G_455S010 100- 9'75 11.49 9-33 ,, 11.87 %1 i i A ' A ' f 1,11'2-14 J A f (AIA i ri-rTl. min 8.00 10.00 12.00 PV by LFiE 08-Jan-13, SV by A P 10-Jan-13. NOTE: O P R Conen. & LOD units = ng/m L, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 147 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Massiynx\inst_G\PFC.PRO\FC\FC2G_455-B.qid Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: WG42273-101,,BLK Name: FC2G__455S010 Description: 1,WG42273,15/4000uL Date: 18-Dec-2012 Time: 20:35:06 13C2-PFOA FC2G 455S010 100- 7.11 f 1.49e5 HI 611324 %~ o7.00 tt rnin 8.00 13C4-PFOA FC2G_455S010 100^ 7.11 F 5.19e4 : \ 212896 % i \ !: '\ 07.00 -T-T min 8.00 13C4-PFOS-1 FC2G 455S010 100- 8.23 r 8.25e3 || 31409 % I; ;: 08.00 10.00 min 12.00 13C4-PFOS-2 FC2G 455S010 8.23 100-3 r 7.21 e3 , 28397 %- | i 0 i t . . ... min 8.00 10.00 12.00 1802-PFHxS-1 FC2G 455S010 100- 7.30 I~7.36e3 30098 %- / 0 7.00 t min 8.00 1802-PFHxS-2 FC2G 455S010 100" 7.30 I~5.69e3 M 22933 %- 07.00 8.00 13C4-PFBA FC2G 455SO10 100- 5.18 T 2,,31e4 1 83825 %- 4.03 o - tt min 5.00 6.00 7.00 13C2-PFHxA FC2G 455S010 100- 6.36 5.55e41 281948 1 !\ o - _____ - j-- min 5,00 6.00 7.00 13C5-PFNA FC2G_j455S010 7.46 100-3 5.10e4 A. 211952 i \ %: I o: 7.00 8.00 13C2-PFDA FC2G_455S010 100-) _ 7.99 5.72e4 210925 % 13C2-PFDoA FC2G 455S010 8.00 10.00 12.00 13C2-PFOUEA FC2G 455S010 PV by LHE 08-Jan-13, SV by AP 10-Jan~13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 148 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnstJ3\PFC.PRO\FC\FC2G_455-B.qid Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: WG42273-101,,BLK Name: FC2G_455S010 Description: 1,WG42273,15/4000uL Date: 18-Dec-2012 Time: 20:35:06 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS~3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHxS-l 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHXA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.20 26 262,98 >218.... 6.06 26 312.97> 268.98 6.37 26 362.97> 31-8.98 6.74 19 412.97> 368,98 7.11 19 412.97> 168.80 27 462.96 >418.... 7.49 28 512.96 >468.... 7.99 28 562.96 >518.... 29 612.95 > 568.... 8.97 23 298.94> 79.96 24 298.94 > 98.95 23 398.84 > 79.96 7.25 24 398.84> 98.96 7.20 23 398.84> 118.70 7.35 21 498.93> 79.96 22 498.93> 98.96 8.23 21 498 >78 9.90 20 414.97 >369.... 7.11 416.97 >371.9 7.11 30 503 > 79.96 8.23 30 503 > 98.96 8.23 30 402,9>84 30 402.9>103 7.30 7.30 30 216.99>171.98 5.18 30 314.97>269.98 6.36 30 467.96>422.97 7.46 30 514.96 >469.96 7.99 30 614.95 > 569..,. 9.03 458.98 >393.98 7.43 Area 460 171 579 3425 231 348 1187 159 70 14 36 39 5 149098 51922 8248 7211 7359 5689 23054 55504 50999 57235 106658 21450 IS Area 23054 55504 55504 55504 149098 149098 50999 57235 57235 106658 7359 5689 7359 5689 7359 8248 7211 8248 51922 21450 21450 21450 21450 21450 21450 21450 21450 21450 ng/L 0.265 0.319 0.096 1.483 0.180 0.539 0.355 67.925 24.981 30.985 36.572 31.835 34.544 20.262 22.659 20.979 19.448 24.669 21.933 LOD 0.0027 0.2877 0.2745 0.1005 0.1406 0.1207 0.5711 0.4803 0.3928 0.0235 0.2142 0.1931 0.2962 0.2296 0.3700 1.4979 0.0350 0.0942 0.1855 0.1059 %Rec S/N 6 4 12 98 5 4 21 3 0 7 0 94.3 104.1 86.1 1 0 1 .6 88.4 96.0 84.4 94.4 87.4 81.0 1 0 2 .8 109.7 3 2 8664 350 460 366 427 283 37 2476 0 572 445 621 Noise 238 206 226 138 180 31 359 194 198 209 19 31 0 6 0 123 56 31 71 608 68 78 71 81 2266 114 0 369 1066 138 Height 1370 825 2698 13572 923 1525 4136 670 294 44 195 160 48 611324 212896 31409 28397 30098 22933 83825 281948 211952 210925 474214 85552 IS Height 83825 281948 281948 281948 611324 611324 211952 210925 210925 474214 30098 22933 30098 22933 30098 31409 28397 31409 212896 85552 85552 85552 85552 85552 85552 85552 85552 85552 Sample size 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 0.5000 Factor2 Detection... 1 .0 MM 1 0 MM 1 .0 MM 1.0 bb 1 0 MMI 1.0 1.0 MM 1.0 MMI 1.0 1.0 MMi 10 10 1.0 MMI 1.0 MM 1 0 MMI 1.0 1.0 MMI 1 .0 MM 1.0 bb 1 0 bb 1.0 bb 1.0 MM 1 0 bb 1.0 bb 1 0 MM 1 0 MM 1 0 bb 1.0 bb 1 .0 bb 1 0 bb PV by LHE 08-Jan-13, SV b y A P 10-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 149 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3GJD15-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time tD: WG42428-101,,BLK Name: FC3GJJ15S022 Description: 1,WG42428,15/4000uL Date: 15^Jan-2013 Time: 01:21:16 PFBA FC3G 015S022 100- 5.23 '.50e2 2758 4.09 V'- .6.34 7.11 5.00 6.00 7.00 PFPeA FC3G015S022 100- 6.42 4.33 6.08 i fMjl 1(6.87 iff 4.95 .11 W1 5.00 6.00 7.00 PFHxA FC3G_015S022 100- 6.36 _ 9.88e2 | 4230 1 6.87 /5.04 5.97 1 ,\ , 5.00 6.00 7.00 PFHpA FC3G__015S022 100-r 6.68 3.05e3 h 11511 H 4.035 43 SM J \ . 5.00 6.00 7.00 PFOA-1 FC3G 015S022 . 7.72 7.00 8.00 PFOA-2 FC3G 015S022 PFNA FC3G 015S022 PFDA FC3G_015S022 100- 7.99 S"T.45e3 1 4513 % 9,3911.16 11.78 1X ' 8.00 10.00 12.00 PFUnA FC3G015S022 100 1 1 .3 4 12.26 \10.32 %- i\ m f\ jv j Vs/ l! 0^8.00 10.00 12.00 PFDoA FC3G_015S022 100- _ 9.21 f~ 3.39e2 | 1592 12.32 %- 8-88l!ffi02i W 0 --if iT.-n-.1_ -r, : , 1 ,,., m j n 8.00 10.00 12.00 PFBS-1 FC3G_015S022 100- 5.86, -4.44 7 1 6.97 V- J A t tin fi \jt A. 1 5.00 6.00 7.00 PFBS-2 FC3G__015S022 100-j4-03 5 3 6 4.74 6.44 s II M \ 7-n 5.00 6.00 7.00 PFHxS-1 FC3G_015S022 7 -a-a 100 7.89e1 A 362 / '^-------T-----i---k-----K-------min 7.00 8.00 PFHxS-2 FC3G 015S022 1 0 0 - 2.18e1 7- 7.33;2.18e1;66 66 : 6.95/ o- 7.00 7 n jT rn -r-r-r mi.n 8.00 PFHxS-3 FC3G 015S022 100-7^6.63 : 2.30e1 ? \ 80 7.00 % - \ 7.90 V\J o - m i , i i i i min 7.00 8.00 PFOS-1 FC3G 015S022 100% f 3.38e2 1 838 12.11 H; 11.13 , ; \ ,. \ o- -/-----v---' ---'----v--"..-.. .' ... min 8.00 10.00 12.00 PFOS-2 FC3G 015S022 100%- i I 9.45 . 1 , n . 7 .. o- j..x : . Ml -n-TT min 8.00 10.00 12.00 PFOSA FC3GJ)15S022 10.08 1001 1 .5 5 & 1 1 10.26 74 11.96 8.11 %- ( 9.42 i Q-* X 8.00 10.00 12.00 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 150 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: WG42428-101,,BLK Name: FC3G_015S022 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 01:21:16 13C2-PFOA FC3G 015S022 100- 7,09 4 2.36e5 4942503 %~ 13C4-PFOA FC3G_015S022 100n _ 7.09 I 9.48e4 1360067 %- 13C4-PFOS-1 FC3G_015S022 100-, _ 8.35 1.35&4 53506 /o~ 13C4-PFOS-2 FC3G__015S022 100- _ 8.35 1.04e4 38459 .i 1802~PFHxS~1 FC3G__015S022 100--1 7.33 .2 0 e 4 l ; 44812 m %- 0- - 77.00 -T-r min 8.00 07.00 8.00 8.00 10.00 12.00 0 - _Lk_ 8.00 10.00 12.00 7.00 8.00 13C4-PFBA FC3G_015S022 100- 5.21 i 3.56e4 j 136463 %- 4.241 5.00 6.00 7.00 13C2-PFHxA FC3G 015S022 100-1 6.34 7.37e4 jj 325908 ;; %- 13C5-PFNA FC3G 015S022 7.49 100^1 8.18e4 ]\ 319316 j \ %- 13C2-PFDA FC3G 015S022 7.99 1 0 0 ^ f 8.54e4 1 300842 %: h o ^o 13C2-PFDoA FC3G_015S022 9.18 1.52e5 655873 %- 0- -- min 5.00 6.00 7.00 o- 7.00 min 8.00 0 - 11 L . min 8.00 10.00 12.00 8.00 10.00 12.00 1802-PFHxS-2 FC3G_015S022 100- 7.33 8.29e3 A ' 32521 j. \ 7.00 13C2-PFOUEA FC3G_015S022 100- 7.43 %- O-H-tt 7.00 8.00 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 151 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: WG42428-101,,BLK Name: FC3GJJ15S022 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 01:21:16 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS~2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFOA 21 13C4-PFOS-1 2 2 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDOA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.23 26 262.98 > 218.... 26 312.97> 268.98 6.36 26 362.97> 318.98 6 .6 8 19 412.97> 368.98 7.09 19 412.97> 168.80 7.14 27 462.96 >418.... 7.49 28 512.96 >468.... 7.99 28 562.96 > 518.... 8.61 29 612.95 >568.... 9.21 23 298.94> 79.96 24 298.94 > 98.95 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.23 2 2 498.93> 98.96 8.29 21 498 > 78 10.08 20 414.97 >369.... 7.09 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.21 30 314.97>269.98 6.34 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.18 458.98 >393.98 7.43 Area 750 988 3051 883 191 153 1447 298 339 79 22 23 338 115 16 235898 94833 13502 10440 11991 8290 35648 73682 81823 85410 152295 28605 IS Area 35648 73682 73682 73682 235898 235898 81823 85410 85410 152295 11991 8290 11991 8290 11991 13502 10440 13502 94833 28605 28605 28605 28605 28605 28605 28605 28605 28605 ng/L 0.093 0.710 0.125 62.969 28.189 38.987 37.942 41.894 39.106 25.374 26.517 26.165 21.710 24.724 20.787 LOD 0.1117 0.2766 0.2744 0.4157 0.0237 0.0189 0.0749 0.0858 %Rec S/N 10 17 69 0 29 0 13 4 7 87.5 117.5 108.3 105.4 116.4 108.6 105.7 110.5 109.0 90.5 103.0 103.9 5 1 3 7 3 8 0 0 463 422 0 0 194 4093 4487 846 1029 0 Noise 275 286 242 168 0 31 0 356 233 213 44 23 67 60 28 121 73 9 0 0 116 91 0 0 705 80 71 356 638 0 Height 2758 4230 11511 2772 916 839 4513 903 1592 362 66 80 838 242 74 942503 360067 53506 38459 44812 32521 136463 325908 319316 300842 655873 103559 IS Height 136463 325908 Sample size 0.5000 0.5000 325908 0.5000 325908 0.5000 942503 0.5000 942503 0.5000 319316 0.5000 300842 0.5000 300842 0.5000 655873 0.5000 44812 0.5000 32521 0.5000 44812 0.5000 32521 0.5000 44812 0.5000 53506 0.5000 38459 0.5000 53506 0.5000 360067 0.5000 0.5000 103559 0.5000 103559 103559 0.5000 0.5000 103559 0.5000 103559 0.5000 103559 0.5000 103559 0.5000 103559 0.5000 103559 0.5000 0.5000 Factor2 Detection... 1 .0 MM 1.0 1.0 MM! 1 .0 MM 1.0 MMI 1.0 bbl 1.0 MMI 1.0 MMI 1.0 MMi 1.0 MM! 1,0 1,0 1.0 MMI 1.0 MMI 1.0 MMI 1.0 MMI 1.0 MM! 1.0 MM 1.0 MM 1 .0 MM 1.0 bb 1.0 bb 1 .0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb PV by LEH 15-Jan-13, SV byA P 16-Jan-13. NOTE: OPR Conen. & LOD units = rig/m L, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 152 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: WG42273-102,,SPM Name: FC2G_455S007 Description: 1,WG42273,15/4000uL Date: 18-Dec-2012 Time: 19:42:48 PFBA FC2G_455S007 loo- cI" .386e4 . 1131606 ty j..... / . . . X - mmin 5.00 6.00 7.00 PFPeA FC2G 455S007 100- 5.48e4 | 283626 1 %~ 8is o-........... .144 min 5.00 6.00 7.00 PFHxA FC2G 455S007 100- 9.79e4~| 484791 :! i'i 0- -............----4---, min 5.00 6.00 7.00 PFHpA FC2G 455S007 10: 1 01e5 '> 432354 %- a 0- K min 5.00 6.00 7.00 PFOA-1 FC2G 455S007 100- 4 8.80e4 ' \ 361588 %" ; > 0 -- ----- ----- tt min 7.00 8.00 PFOA-2 FC2G_455S007 7.11 100T ;T 2.41 e4 ; 99768 %- 0- 7.00 8.00 PFNA FC2G__455S007 100- 9.22e4 I 399544 1 / ' 4 . 7 7 min 7.00 8.00 PFDA FC2G 455S007 1 0 0 - F 1-11e5 j 404793 %: i. o- min 8.00 10.00 12.00 PFUnA FC2G_455S007 100- f 1.27e5 [j 557533 %- i 0 M 0 , i l l . .......'... ..... min 8.00 10.00 12.00 PFDoA FC2G 455S007 107 F 1.53e5 | 668149 %- I 0 - 1 nr min 8.00 10.00 12.00 PFBS-1 FC2G 455S007 10 - 2.56e4 ` 126716 %z j; ; * min 5.00 6.00 7.00 PFBS-2 FC2G_455S007 1 0 0 -} 6.47 1.39e4 h 65901 \ %- o5.00 6.00 7.00 PFHxS-1 FC2G_455S007 100 2.12e4 J \ 76588 -f min 7.00 8.00 PFHxS-2 FC2G 455S007 .10 0 - ' 1.18e4 / \ 43383 %- o- -- 1 -- 7.00 -rmin 8.00 PFHxS-3 FC2G 455S007 400- \ 7.28e3 / '; 26303 %~ 0J ,illfew -- min 7.00 8.00 PFOS-1 FC2G 455S007 1 00" r 1.95e4 1 53920 0TM 8.00 9.90 10.00 min 12.00 PFOS-2 FC2G 455S007 1 0 0 - ! 1.35e4 H 44854 %z 0 -* 8.00 min 10.00 12.00 PFOSA FC2G 455S007 1 00- 2.36e4 1 80783 !! %: 0 -* - ....- - 4 - 8.00 10.00 12.00 P V by L H E 0 8 -J a n -1 3 , S V by A P 10 -Jan -13 . N O T E : O P R Conen. & L O D units = ng/m L, S am p le and Lab BLK Conen. & LO D units = ng/L. Page 153 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Massiynx\lnst_G\PFC.PRO\FC\FC2G..455-B.qld Last Altered: Tuesday. January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: WG42273-102,,SPM Name: FC2G_455S007 Description: 1,WG42273,15/4000uL Date: 18-Dec-2012 Time: 19:42:48 13C2-PFOA FC2G 455S007 100n 7.11 f 1.51e5 | 4 616888 %~ 13C4-PFOA FC2G 455S007 100- 7.11 f 5.36e4 M 214360 %- 13C4-PFOS-1 FC2G 455S007 100- 8.23 f 8.56e3 | 30493 %- ; 0- ' x .. . min 7.00 8.00 0- 7 x -. -n min 7.00 8.00 o8.00 10.00 12.00 13C4-PFBA FC2G 455S007 13C2-PFHxA FC2G_455S007 100n 6.34 5.39e4 281173 % 13C5-PFNA FC2G 455S007 5.00 6.00 7,00 13C4-PFOS-2 FC2G 455S007 100n 8.23 I 6.39e3 I 23217 %~ 1| || o8.00 10.00 , min 12.00 1802-PFHxS-1 FC2G 455S007 100-j 7.30 f 7.93e3 30189 /r / 0- 7.00 .. . min 8.00 1802-PFHXS-2 FC2G 455S007 100- 7.25 A 5.98e3 / \ 23816 %- 07.00 8.00 13C2-PFDA FC2G_455S007 100- 7.93 5.49e4 201955 13C2-PFDoA FC2G__455S007 100- 9.06 r 9.83e4 446526 13C2-PFOUEA FC2G 455S007 0 ^-- 8.00 10.00 12.00 8.00 10.00 12.00 PV by LHE 08-Jan-13, SV by AP 10-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 154 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G :\M a s s ly n x \ln s t_ G \P F C .P R 0 \F C \F C 2 G ..455-B .qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: WG42273-102,,SPM Name: FC2G_455S007 Description: 1,WG42273,15/4000uL Date: 18-Dec-2012 Time: 19:42:48 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2~PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212 9 9> 168.98 5 11 26 262.98 >218.... 5.95 26 3 12.97> 268.98 6.34 26 362.97> 318,98 6.69 19 4 1 2 .9 7> 368 .98 7.11 19 412.97> 168.80 7.11 27 462.96 >418.... 7.46 28 512.96 >468.... 7.93 28 562.96 >518.,.. 8.50 29 612.95 > 568.... 9.06 23 298.94> 79.96 6.45 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.30 23 398.84> 118.70 7.30 21 4 9 8 .9 3 > 79.9 6 8.23 22 498.93 > 98.96 8.23 21 498 > 78 9.90 20 414.97 > 369.... 7.11 416.97 >371.9 7.11 30 503 > 79.96 8.23 30 503 > 98.96 8.23 30 402.9>84 7.30 30 402.9>103 7.25 30 216.99>171.98 5.11 30 314.97>269,98 6.34 30 467.96>422.97 7.46 30 514.96 >469,96 7.93 30 614.95 > 569.... 9.06 458.98 >393.98 7.39 Area 38623 54795 97900 101464 88021 24078 92152 110967 126707 152836 25582 13860 21175 11791 7278 19513 13536 23624 150918 53590 8562 6394 7930 5976 23236 53882 53397 54892 98261 20751 IS A rea 23236 53882 53882 53882 150918 150918 53397 54892 54892 98261 7930 5976 7930 5976 7930 8562 6394 8562 53590 20751 20751 20751 20751 20751 20751 20751 20751 20751 ng/L 19.001 17.749 19.999 19.031 19.476 20.396 19.805 22.765 20.906 20.176 43.287 39.868 40.075 36.686 41.211 38.784 43.532 22.482 33.307 12.892 16.622 16.760 17.729 18.756 10.555 11.369 11.352 9.640 11.746 10.609 LOD 0.0476 0.1425 0.1427 0.0310 0.0422 0.0974 0.3304 0.2250 0.3883 0.2360 0.1440 0.1411 0.0971 0.0431 0.1417 0.0128 0.0082 0.0619 0.0992 0.1256 %Rec 95.0 88.7 1 0 0 .0 95.2 97.4 1 0 2 .0 99.0 113.8 104.5 100.9 108.2 99.7 1 0 0 .2 91.7 103.0 97.0 108.8 112.4 92.5 107.4 92.3 93.1 98.5 104.2 8 8 .0 94.7 94.6 80.3 97.9 106.1 S/N 429 1429 5471 2295 0 0 1979 1080 1195 2966 2429 1243 0 0 0 157 384 1021 0 269 313 468 0 1295 176 3468 4426 428 401 253 N o is e 307 198 89 188 0. 0 202 375 466 225 52 53 0 0 0 343 117 79 0 798 97 50 0 18 418 81 52 472 1112 329 H e ig h t 131606 283626 484791 432354 361588 99768 399544 404793 557533 668149 126716 65901 76588 43383 26303 53920 44854 80783 616888 214360 30493 23217 30189 23816 73392 281173 229687 201955 446526 83449 IS Height 73392 281173 281173 281173 616888 616888 229687 201955 201955 446526 30189 23816 30189 23816 30189 30493 23217 30493 214360 83449 83449 83449 83449 83449 83449 83449 83449 83449 S am ple size 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1.0000 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 Factor2 Detection... 1 .0 bh 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 b.b 1,0 bb 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1,0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1 .0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb PV by LHE 08-Jan-13, SV byAP 10-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 155 of 307 www.axysanalytical.com Quantify Sample Report MassLynx4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: WG42428-102,,SPM Name: FC3G_015S019 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 00:30:21 oo PFBA FC3G 015S019 5.19 F 6.14e4 1 242972 !\ 0 - rrr min 5.00 6.00 7.00 PFPeA FC3G 015S019 10 On 5.93 7.67e4 1 327328 1 %: 0- J [j min 5.00 6.00 7.00 PFHxA FC3G 015S019 100- 6.33 1.46e5l 655296 I; %~ 0 min 5.00 6.00 7.00 PFHpA FC3G_015S019 1 0 0 -j 6.73 _ 1.40e5 , 568185 | %. 05.00 6.00 7.00 PFNA FC3GJ)15S019 PFDA FC3G_015S019 7.99 100^ fl.6 8 e 5 | 602678 PFUnA FC3G_015S019 100- ,, 8-58 jp1.88e5 1 842752 % PFDoA FC3G_015S019 F100 9.18 2:..445e5 I 1086839 &L 8.00 10.00 12.00 PFHxS-1 FC3G 015S019 100- 7.33 jT 3.46e4 m 128809 %7 0: J 7.00 v .. -r min 8.00 PFHxS-2 FC3G_015S019 7.33 1 0 0 1 1.86e4 A 67739 A %- o7.00 8.00 Ml 8.00 10.00 12.00 PFHxS-3 FC3G 015S019 8.00 10.00 12.00 PFOS-1 FG3G 015S019 PFOA-1 FC3G_015S019 100- 7.09 1.46e5 ,579316 7.00 8.00 PFBS-1 FC3G_015S019 100- 6.47 _ 3.95e4 175396 %- :------- 5.00 6.00 7.00 PFOS-2 FC3G_015S019 100- _ 8.35 2.25e4 70707 %- 11 - 8.00 10.00 12.00 PFOA-2 FC3GJM5S019 100- 7.09 !'\ 3.37e4 / \ 135987 7.00 8.00 PFBS-2 FC3G_015S019 100^ 6.47 2.07e4 90863 5.00 6.00 7.00 PFOSA FC3G_015S019 9.99 100- 2.60e4 | 91252 I %-- 8.00 10.00 12.00 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 156 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4,1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-B.qid Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: WG42428-102,,SPM Name: FC3G_015S019 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 00:30:21 13C2-PFOA FC3G 015S019 1o-q 7.09 i2 .1 7 e 5 Q861524 %" 13C4-PFOA FC3G 015S019 100- 7.09 I " 9.14e4 f \ 373243 %- 0 - -- -------- ------ m t -t min 7.00 8.00 i 7.00 | 8.00 13C4-PFBA FC3G_015S019 5.21 100- IT3.59e4 A 138692 %- .4.03 13C2-PFHxA FC3G_015S019 6.33 _ 100- 6;85e4 309223 %- 5.00 6.00 7.00 5.00 6.00 7.00 13C4-PFOS-1 FC3G_015S019 8.35 1001 1.20e4 44307 %: 08.00 10.00 12.00 13C5-PFNA FC3G_015S019 7.49 100- 7.52e4 A 304183 ! \ %- 7.00 8.00 13C4-PFOS-2 FC3GJ315S019 100- _ 8.35 I 9.85e3 I 36201 8.00 10.00 12.00 13C2-PFDA FC3GJD15S019 100- 7.99 T 8.25e4 f: 293260 %- 8.00 10.00 12.00 1802-PFHXS-1 FC3G_015S019 100~- 7.33 _ 1.14e4l| ' 44529 Q %-- 7.00 8.00 13C2-PFDoA FC3GJ015S019 100-, 9.18 f " 1.50e5 I 668909 8.00 10;00 12.00 1802-PFHxS-2 FC3G_015S019 100- 7.29 f7.93e3 . 29137 %- 7.00 8.00 13C2-PFOUEA FC3GJJ15S019 7.38 100-2.98e4 IS 113697 ; \ %- /\ ----- J.-- ---- . 7.00 8.00 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: O PR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 157 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4,1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: WG42428-102,,SPM Name: FC3G_015S019 Description: 1,WG42428,15/4000uL Date: 15-Jan-2013 Time: 00:30:21 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFB.S-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFFixS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168,98 5.19 26 262.98 > 218.... 5.93 26 312.97> 268.98 6.33 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.49 28 512.96 >468.... 7.99 28 562.96 >518.... 8.58 29 612.95 >568.... 9.18 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.35 22 498.93> 98.96 8.35 21 498 > 78 9.99 20 414.97 > 369.... 7.09 416.97 >371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.29 30 216.9 9>171.98 5.21 30 314.97>269.98 6.33 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.18 458.98 >393.98 7.38 Area 61431 76675 145635 140305 145709 33686 134348 167763 188112 245386 39499 20661 34616 18554 10103 33380 22503 26025 217149 91367 11956 9846 11355 7931 35927 68491 75185 82535 149576 29758 IS Area 35927 68491 68491 68491 217149 217149 75185 82535 82535 149576 11355 7931 11355 7931 11355 11956 9846 11956 91367 29758 29758 29758 29758 29758 29758 29758 29758 29758 ng/L 19.238 19.201 22.266 21.141 23.709 22.684 21.815 22.345 23.057 22.506 44.406 44.042 43.108 41.942 41.538 46.832 42.920 23.726 30.081 13.579 16.594 17.199 19.067 17.981 12.291 11.847 11.556 10.083 11.671 10.812 LOD 0.0133 2.0076 0.1034 0.0757 0.1837 0.1068 0.1062 0.3346 0.0948 0.0181 0.0058 0.0483 0.1077 %Rec 96.2 96.0 111.3 105.7 118.5 113.4 109.1 111.7 115.3 112.5 111.0 110.1 107.8 104.9 103.8 117.1 107.3 118.6 83.6 113.2 92.2 95.6 105.9 99.9 102.4 98.7 96.3 84.0 97.3 108.1 S/N 692 973 2870 1760 0 524 2350- 1508 2553 1648 3174 5257 0 3256 49 398 315 2656 0 222 452 468 175 0 393 2318 6290 583 380 0 Noise 351 337 228 323 0 260 229 400 330 660 55 17 0 21 728 240 224 34 0 1683 98 77 254 0 353 133 48 503 1758 0 Height 242972 327328 655296 568185 579316 135987 537357 602678 842752 1086839 175396 90863 128809 67739 35944 95358 70707 91252 861524 373243 44307 36201 44529 29137 138692 309223 304183 293260 668909 113697 IS Height 138692 309223 309223 309223 861524 861524 304183 293260 293260 668909 44529 29137 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 ' 1.0000 1.0000 1.0000 44529 29137 44529 '1,0000 1.0000 1.0000 44307 36201 44307 373243 113697 113697 113697 113697 113697 113697 113697 113697 113697 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb PV by LEH 15-Jan-13, SV b y A P 16-Jan-13. NOTE: O PR Conen. & LOD units = ng/mL, Sam ple and Lab BLK Conen. & LOD units = ng/L. Page 158 of 307 www.axysanalytical.com __________________________ PFC _____________________ Sample List Report M assLynx4.1 SCN627 Sample List: G:\Massiynx\lnst_G\_Master.PRO\SampleDB\FC2G_445.SPL Last Modified: Thursday, December 13, 2012 11:30:57 Pacific Standard Time Printed: Thursday, December 13, 2012 14:52:46 Pacific Standard Time Page 1 of 1 Page Position (1,1 ) File Name Sample ID File Text Bottle 1 FC2G_445S001 MeOH,, . 2 FC2G_445S002 MeOH,, 3 FC2G_445S003 FC-LlN-42221 W500-A,, 4 FC2G_445S004 FC-LIN-42221W500-A,, 5 FC2G_445S005 FC-LIN-42221W500-B,, 6 FC2G_445S006 FC-LIN-42221 W500-C,, 7 FC2G_445S007 FC-LIN-42221 W500-D,, 8 FC2G_445S008 FC-LIN-42221 W500-E,, 9 FC2G_445S009 FC-LIN-42221 W500-F,, 10 FC2G_445S010 FC-LIN-42221 W500-G,. 11 FC2G_445S011 FC-LIN-42221 W500-H,, 12 FC2G_445S012 MeOH,, 13 FC2G_445S013 MeOH,, 14 FC2G_445S014 FC-42221 W500-SAR2,, 15 FC2G_445S015 FC-42221W500-SAR,, 16 FC2G_445S016 FC-LIN-42221 W500-D,, 17 FC2G_445S017 FC-LIN-42221 W500-D,, 18 FC2G_445S018 WG42214-103,,SAR 19 FC2G_445S019 WG42214-102,,SPM 20 FC2G_445S020 MeOH,, 21 FC2G_445S021 MeOH,, 22 FC2G_445S022 WG42214-101,,BLK 23 FC2G_445S023 L18938-1,, 24 FC2G_445S024 L18938-2,, 25 FC2G_445S025 L18938-3,, 26 FC2G_445S026 L18955-1,, 27 FC2G_445S027 L18955-2,, 28 FC2G_445S028 L18955-3,, 29 FC2G_445S029 L18955-4,, 30 FC2G__445S030 L18955-5,, 31 FC2G_445S031 L18955-6,, 32 FC2G_445S032 L18955-7,, 33 FC2G_445S033 L18970-1,, 34 FC2G_445S034 L18970-2,, 35 FC2G_445S035 L18970-3,, 36 FC2G_445S036 L18970-4,, 37 FC2G_445S037 FC-LIN-42221 W500-D,, 38 FC2G_445S038 FC-LIN-42221 W500-D,, 39 FC2G_445S039 MeOH,, 40 FC2G 445S040 MeOH,, 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,,T5uL 1,,15uL 1,,15u L 1 ,,1 5uL 1.. 15uL 1.. 15uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1.WG42214,15/4000uL 1,WG42214,15/4000uL 1,,15uL 1,,15uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,WG42214,15/4000uL 1,,15uL 1,,15uL 1,,15uL 1,,15uL 1:8,A 1:8,A 1:1,A 1:1,A 1:1,B 1:1,C 1:1,D 1:1,E 1:1,F 1:2,A 1:2,B 1:8,A 1:8,A 1:2, C 1:2,D 1:1,D 1:1,D 1:2,E 1:2,F 1:8,A 1:8,A 1:6,E 1:6,F 1:7,A 1:7,B 1:7, C 1:7,D 1:7,E 1:7,F 1:8,B 1:8,C 1:8,D 1:8,E 1:8,F 1:4,E 1:4,F 1:1,D 1:1,D 1:8, A 1:8,A "77 w S ig n O u t aM E- Inj VoL 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 Page 159 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Method: G:\Masslynx\Master Methods\PFC\FC_060_10_01.mdb 03 Dec 2010 17:21:03 Calibration: 14 Dec 2012 11:01:10 Compound name: PFBA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 5.13 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 5.16 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 5.14 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 5.18 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 5.11 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 5.14 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 5.11 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 5.20 Area 1702 3260 11699 42289 213591 421406 953630 1826622 IS Area 29460 25526 29193 27354 24645 25200 21251 16912 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.567 1.371 4.508 17.665 99.497 192.121 516.166 1244.856 %Rec 113.5 109.7 90.2 88.3 99.5 96.1 103.2 99.6 Compound name: PFBA Coefficient of Determination: RA2 = 0.999416 Calibration curve: -2.74527e-006 * xA2 + 1.0445 * x + 0.100781 Response type: Internal Std ( Ref 25 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 30 40 49 90 2015 2159 3615 12024 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 160 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFPeA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 5.96 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 5.96 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 5.98 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 5.98 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 5.96 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 5.98 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 5.95 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 5.98 Area 1948 4081 15995 60142 293936 566115 1246496 2438345 IS Area 66835 60698 64980 58266 53679 48323 43496 35274 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.611 1.272 4.375 18.014 95.340 204.560 505.025 1247.545 %Rec 122.3 101.7 87.5 90.1 95.3 102.3 101.0 99.8 Compound name: PFPeA Coefficient of Determination: RA2 = 0.999656 Calibration curve: -2.17e-005 * xA2 + 0.692047 * x + -0.0732216 Response type: Internal Std ( Ref 26 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 86 92 325 742 10920 11971 41155 36887 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 161 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFHxA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 6.37 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 6.36 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 6.37 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 6.37 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 6.36 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 6.36 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 6.32 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 6.36 Area 3763 7458 27050 100408 472991 888540 1813293 3315686 IS Area 66835 60698 64980 58266 53679 48323 43496 35274 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.553 1.286 4.521 18.962 98.317 208.462 492.664 1252.147 %Rec 110.6 102.9 90.4 94.8 98.3 104.2 98.5 100.2 Compound name: PFHxA Coefficient of Determination: RA2 = 0.999663 Calibration curve: -0.000150762 * xA2 + 1.08956 * x + 0.0730969 Response type: Internal Std ( Ref 26 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 85 211 611 3750 10164 21372 61073 44296 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 162 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFHpA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 6.74 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 6.74 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 6.74 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 6.74 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 6.74 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 6.74 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 6.69 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 6.74 Area 3179 6544 28341 104254 530786 1004495 2055822 4086324 IS Area 66835 60698 64980 58266 53679 48323 43496 35274 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.600 1.204 4.498 18.089 99.883 211.217 488.032 1253.279 %Rec 119.9 96.3 90.0 90.4 99.9 105.6 97.6 100.3 Compound name: PFHpA Coefficient of Determination: RA2 = 0.999397 Calibration curve: -6.94479e-005 * xA2 + 1.19636 * x + -0.14655 Response type: Internal Std ( Ref 26 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 56 77 548 1098 11928 13584 22109 87183 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 163 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFOA-1 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.11 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.11 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.11 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.11 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.11 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.11 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.06 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.11 Area 3480 6169 25268 94311 452340 853031 1776266 3356814 IS Area 178974 163290 176604 164434 147281 145888 125019 100831 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.565 1.179 4.707 19.151 103.692 199.162 497.215 1251.114 %Rec 113.0 94.3 94.1 95.8 103.7 99.6 99.4 100.1 S/N 85 32 0 1407 0 0 0 0 Compound name: PFOA-1 Coefficient of Determination: RA2 = 0.999878 Calibration curve: -9.37217e-005 * xA2 + 1.07512 * x + 0.092653 Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 164 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFOA-2 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.11 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.11 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.11 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.11 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.11 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.11 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.06 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.11 Area 659 1818 6596 22890 113491 221628 480485 888554 IS Area 178974 163290 176604 164434 147281 145888 125019 100831 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.500 1.450 4.793 17.796 99.005 196.690 510.440 1245.993 %Rec 100.0 116.0 95.9 89.0 99.0 98.3 102.1 99.7 S/N 50 96 0 0 0 0 0 0 Compound name: PFOA-2 Coefficient of Determination: RA2 = 0.999698 Calibration curve: -2.23765e-005 * xA2 + 0.282497 * x + -0.00875009 Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... bb bb bb bb bb bb bb bb PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 165 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFNA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.49 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.46 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.49 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.49 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.49 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.49 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.46 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.49 Area 2996 6297 26363 95907 471902 917096 1926313 3754168 IS Area 61446 64296 62761 57763 52387 51976 44006 33246 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.571 1.136 4.832 19.055 103.082 201.344 495.377 1251.356 %Rec 114.3 90.9 96.6 95.3 103.1 100.7 99.1 100.1 S/N 31 258 1068 2531 0 0 0 0 Compound name: PFNA Coefficient of Determination: RA2 = 0.999897 Calibration curve: 2.97143e-005 * xA2 + 1.04569 * x + -0.0125033 Response type: Internal Std ( Ref 27 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 166 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFDA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.99 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.93 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.99 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.99 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.99 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.99 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.93 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.99 Area 4595 9683 30905 115554 552400 1032872 2264803 4575060 IS Area 70921 65473 67478 62623 63392 57749 60934 58399 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.426 1.370 4.896 20.743 101.028 213.460 474.551 1264.185 %Rec 85.2 109.6 97.9 103.7 101.0 106.7 94.9 101.1 S/N 32 133 286 1048 1346 4803 5790 6641 Compound name: PFDA Coefficient of Determination: RA2 = 0.998772 Calibration curve: -0.00024797 * xA2 + 1.05686 * x + 0.327338 Response type: Internal Std ( Ref 28 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 167 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFUnA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 8.50 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 8.50 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 8.53 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 8.53 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 8.53 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 8.50 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 8.50 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 8.56 Area 3886 9165 35480 126694 671038 1344974 2816578 5587121 IS Area 70921 65473 67478 62623 63392 57749 60934 58399 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.528 1.294 4.770 18.316 97.651 221.955 472.106 1265.057 %Rec 105.6 103.6 95.4 91.6 97.7 111.0 94.4 101.2 Compound name: PFUnA Coefficient of Determination: RA2 = 0.997578 Calibration curve: -0.000337294 * xA2 + 1.33425 * x + -0.0467692 Response type: Internal Std ( Ref 28 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 60 88 384 1322 2940 7104 10581 17560 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 168 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFDoA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 9.09 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 9.09 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 9.12 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 9.09 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 9.12 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 9.09 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 9.09 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 9.12 Area 4341 9683 38200 144155 706897 1522557 3461389 6343367 IS Area 93234 84855 100297 91532 94481 101933 110521 112180 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.463 1.340 4.808 20.433 100.284 208.066 484.187 1269.169 %Rec 92.6 107.2 96.2 102.2 100.3 104.0 96.8 101.5 Compound name: PFDoA Coefficient of Determination: RA2 = 0.999521 Calibration curve: -0.000307507 * xA2 + 0.924823 * x + 0.130548 Response type: Internal Std ( Ref 29 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 49 126 428 1897 8277 12111 10729 16794 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 169 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFBS-1 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 6.49 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 6.47 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 6.49 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 6.49 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 6.47 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 6.49 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 6.45 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 6.47 Area 944 1769 6729 26283 125842 219038 439326 780518 IS Area 9306 8155 9974 9157 7654 7157 6135 4060 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 1.027 2.589 8.782 38.475 221.475 411.492 956.132 2513.525 %Rec 102.7 103.6 87.8 96.2 110.7 102.9 95.6 100.5 Compound name: PFBS-1 Coefficient of Determination: RA2 = 0.998845 Calibration curve: 1.84294e-005 * xA2 + 1.33009 * x + 0.460742 Response type: Internal Std ( Ref 23 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 177 199 928 6178 15154 32828 44106 61314 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 170 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFBS-2 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 6.49 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 6.49 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 6.49 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 6.49 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 6.47 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 6.47 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 6.43 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 6.47 Area 370 922 3517 13781 66054 115161 225115 390955 IS Area 7048 6816 6816 6371 5206 5242 3840 2738 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 1.114 2.529 9.035 37.200 217.479 376.960 1011.489 2497.700 %Rec 111.4 101.2 90.4 93.0 108.7 94.2 101.1 99.9 Compound name: PFBS-2 Coefficient of Determination: RA2 = 0.999182 Calibration curve: -9.57371e-006 * xA2 + 1.05321 * x + -0.22778 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 32 136 416 2829 7804 23419 34911 45765 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... bb bb bb bb bb bb bb bb PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 171 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFHxS-1 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.30 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.30 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.30 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.30 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.30 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.30 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.30 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.30 Area 843 1763 5780 21905 106920 198806 395234 689052 IS Area 9306 8155 9974 9157 7654 7157 6135 4060 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 Compound name: PFHxS-1 Coefficient of Determination: RA2 = 0.999195 Calibration curve: 1.13524e-005 * xA2 + 1.1886 * x + 0.41678 Response type: Internal Std ( Ref 23 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None ng/L 1.021 2.923 8.425 35.862 210.774 418.652 966.396 2509.441 %Rec 102.1 116.9 84.2 89.7 105.4 104.7 96.6 100.4 S/N 228 0 0 0 0 0 0 0 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 172 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFHxS-2 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.30 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.25 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.30 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.30 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.30 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.30 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.25 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.30 Area 351 883 3235 12711 57268 104543 213611 356435 IS Area 7048 6816 6816 6371 5206 5242 3840 2738 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 1.144 2.617 8.991 37.095 203.944 370.394 1042.150 2487.173 %Rec 114.4 104.7 89.9 92.7 102.0 92.6 104.2 99.5 Compound name: PFHxS-2 Coefficient of Determination: RA2 = 0.998903 Calibration curve: -1.29242e-005 * xA2 + 0.974532 * x + -0.219288 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 34 0 0 0 10638 7432 0 0 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... bb bb bb bb bb bb bb bb PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 173 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFHxS-3 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.30 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.30 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.30 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.30 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.30 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.30 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.25 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.30 Area 301 530 1815 7686 34687 65881 129524 214852 IS Area 9306 8155 9974 9157 7654 7157 6135 4060 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 1.163 2.635 7.926 37.658 205.297 418.995 971.484 2508.404 %Rec 116.3 105.4 79.3 94.1 102.6 104.7 97.1 100.3 S/N 75 520 205 0 0 0 0 0 Compound name: PFHxS-3 Coefficient of Determination: RA2 = 0.999378 Calibration curve: -7.42791e-006 * xA2 + 0.398291 * x + 0.119303 Response type: Internal Std ( Ref 23 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... bb bb bb bb bb bb bb bb PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 174 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFOS-1 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 8.23 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 8.23 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 8.29 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 8.23 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 8.23 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 8.23 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 8.23 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 8.23 Area 763 1662 5671 20621 110728 201138 440275 786051 IS Area 10054 10200 10723 9486 9214 8227 7488 5039 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 1.110 2.600 8.861 36.986 204.433 413.788 980.906 2504.839 %Rec 111.0 104.0 88.6 92.5 102.2 103.4 98.1 100.2 S/N 23 23 18 320 936 1500 2000 2485 Compound name: PFOS-1 Coefficient of Determination: RA2 = 0.999693 Calibration curve: 2.7728e-005 * xA2 + 1.05152 * x + 0.198925 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 175 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFOS-2 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 8.23 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 8.23 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 8.23 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 8.23 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 8.29 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 8.23 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 8.23 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 8.29 Area 524 948 3809 14211 72388 133090 280510 532049 IS Area 7580 6871 7462 8114 6988 6470 5370 3443 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 1.033 2.468 10.226 35.976 210.588 409.504 979.408 2504.844 %Rec 103.3 98.7 102.3 89.9 105.3 102.4 97.9 100.2 S/N 17 39 118 338 856 1370 1853 1977 Compound name: PFOS-2 Coefficient of Determination: RA2 = 0.999675 Calibration curve: 9.87089e-005 * xA2 + 0.862929 * x + 0.352919 Response type: Internal Std ( Ref 22 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... bb bb bb bb bb bb bb bb PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 176 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: PFOSA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 9.90 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 9.87 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 9.90 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 9.90 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 9.93 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 9.90 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 9.90 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 9.93 Area 542 1454 5000 21170 112866 229818 560574 1006244 IS Area 10054 10200 10723 9486 9214 8227 7488 5039 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.613 1.336 3.974 18.251 96.015 208.426 497.907 1088.040 %Rec 122.5 106.9 79.5 91.3 96.0 104.2 99.6 87.0 S/N 68 278 192 1567 1898 4612 6474 4989 Compound name: PFOSA Coefficient of Determination: RA2 = 0.999004 Calibration curve: 0.00101176 * xA2 + 2.20331 * x + -0.379861 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MMX PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 177 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C2-PFOA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.11 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.11 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.11 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.11 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.11 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.11 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.06 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.11 Area 178974 163290 176604 164434 147281 145888 125019 100831 Compound name: 13C2-PFOA Response Factor: 1.01462 RRF SD: 0.0551777, % Relative SD: 5.43827 Response type: Internal Std ( Ref 20 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 62157 54677 61363 56742 47889 45943 40127 30166 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/L 34.055 35.321 34.039 34.274 36.374 37.556 36.848 39.533 %Rec 94.6 98.1 94.6 95.2 101.0 104.3 102.4 109.8 S/N 1348 0 0 2093 0 0 0 0 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 178 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C4-PFOA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.11 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.11 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.11 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.11 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.11 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.11 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.06 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.11 Compound name: 13C4-PFOA Response Factor: 4156.9 RRF SD: 921.465, % Relative SD: 22.1671 Response type: External Std, Area Curve type: Rf Area 62157 54677 61363 56742 47889 45943 40127 30166 IS Area Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 14.953 13.153 14.762 13.650 11.520 11.052 9.653 7.257 %Rec 124.6 109.6 123.0 113.8 96.0 92.1 80.4 60.5 S/N 0 278 342 0 0 0 0 0 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 179 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C4-PFOS-1 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 8.23 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 8.23 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 8.29 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 8.23 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 8.23 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 8.23 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 8.23 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 8.29 Area 10054 10200 10723 9486 9214 8227 7488 5039 Compound name: 13C4-PFOS-1 Response Factor: 0.248214 RRF SD: 0.0245457, % Relative SD: 9.88892 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 22841 20301 23325 21380 18798 18995 16675 14160 Std. Conc 18.000 18.000 18.000 18.000 18.000 18.000 18.000 18.000 ng/L 17.734 20.243 18.522 17.876 19.747 17.451 18.093 14.336 %Rec 98.5 112.5 102.9 99.3 109.7 96.9 100.5 79.6 S/N 400 499 438 506 576 277 293 195 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 180 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C4-PFOS-2 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 8.23 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 8.23 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 8.29 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 8.29 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 8.23 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 8.23 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 8.23 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 8.29 Area 7580 6871 7462 8114 6988 6470 5370 3443 Compound name: 13C4-PFOS-2 Response Factor: 0.183845 RRF SD: 0.0230781, % Relative SD: 12.553 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 22841 20301 23325 21380 18798 18995 16675 14160 Std. Conc 18.000 18.000 18.000 18.000 18.000 18.000 18.000 18.000 ng/L 18.052 18.408 17.402 20.643 20.221 18.527 17.518 13.227 %Rec 100.3 102.3 96.7 114.7 112.3 102.9 97.3 73.5 S/N 110 234 297 390 226 201 285 91 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... bb bb bb bb bb bb bb bb PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 181 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 18O2-PFHxS-1 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.30 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.30 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.30 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.30 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.30 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.30 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.30 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.30 Area 9306 8155 9974 9157 7654 7157 6135 4060 Compound name: 18O2-PFHxS-1 Response Factor: 0.215533 RRF SD: 0.0256414, % Relative SD: 11.8967 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 22841 20301 23325 21380 18798 18995 16675 14160 Std. Conc 18.000 18.000 18.000 18.000 18.000 18.000 18.000 18.000 ng/L 18.904 18.638 19.840 19.872 18.891 17.481 17.070 13.304 %Rec 105.0 103.5 110.2 110.4 105.0 97.1 94.8 73.9 S/N 409 822 0 943 0 0 1927 0 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 182 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 18O2-PFHxS-2 # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.30 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.30 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.30 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.30 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.30 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.30 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.25 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.30 Area 7048 6816 6816 6371 5206 5242 3840 2738 Compound name: 18O2-PFHxS-2 Response Factor: 0.153548 RRF SD: 0.0251138, % Relative SD: 16.3557 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 22841 20301 23325 21380 18798 18995 16675 14160 Std. Conc 18.000 18.000 18.000 18.000 18.000 18.000 18.000 18.000 ng/L 20.097 21.866 19.031 19.406 18.038 17.974 14.997 12.590 %Rec 111.7 121.5 105.7 107.8 100.2 99.9 83.3 69.9 S/N 0 423 0 619 251 0 0 0 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... bb bb bb bb bb bb bb bb PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 183 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C4-PFBA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 5.13 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 5.14 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 5.14 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 5.18 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 5.13 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 5.14 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 5.07 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 5.16 Area 29460 25526 29193 27354 24645 25200 21251 16912 Compound name: 13C4-PFBA Response Factor: 1.06091 RRF SD: 0.0338595, % Relative SD: 3.19155 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 22841 20301 23325 21380 18798 18995 16675 14160 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 12.158 11.852 11.797 12.060 12.358 12.505 12.013 11.257 %Rec 101.3 98.8 98.3 100.5 103.0 104.2 100.1 93.8 S/N 196 284 213 242 140 82 75 62 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 184 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C2-PFHxA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 6.37 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 6.36 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 6.36 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 6.36 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 6.36 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 6.36 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 6.32 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 6.36 Area 66835 60698 64980 58266 53679 48323 43496 35274 Compound name: 13C2-PFHxA Response Factor: 2.284 RRF SD: 0.151209, % Relative SD: 6.62038 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 22841 20301 23325 21380 18798 18995 16675 14160 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 12.812 13.091 12.197 11.932 12.503 11.138 11.421 10.906 %Rec 106.8 109.1 101.6 99.4 104.2 92.8 95.2 90.9 S/N 3315 3192 5218 1610 3043 2177 1922 2803 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 185 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C5-PFNA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.46 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.46 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.49 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.49 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.46 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.49 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.46 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.49 Area 61446 64296 62761 57763 52387 51976 44006 33246 Compound name: 13C5-PFNA Response Factor: 2.26666 RRF SD: 0.186811, % Relative SD: 8.2417 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 22841 20301 23325 21380 18798 18995 16675 14160 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 11.869 13.972 11.871 11.920 12.295 12.072 11.643 10.358 %Rec 98.9 116.4 98.9 99.3 102.5 100.6 97.0 86.3 S/N 4801 5026 2521 4915 2428 4295 787 0 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 186 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C2-PFDA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.99 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.93 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.99 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.99 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.99 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.99 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.93 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.99 Area 70921 65473 67478 62623 63392 57749 60934 58399 Compound name: 13C2-PFDA Response Factor: 2.74408 RRF SD: 0.348433, % Relative SD: 12.6976 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 22841 20301 23325 21380 18798 18995 16675 14160 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 11.315 11.753 10.543 10.674 12.289 11.079 13.317 15.029 %Rec 94.3 97.9 87.9 89.0 102.4 92.3 111.0 125.2 S/N 678 870 601 594 502 368 384 531 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 187 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C2-PFDoA # ID 1 FC-LIN-42221W500-A,, 2 FC-LIN-42221W500-B,, 3 FC-LIN-42221W500-C,, 4 FC-LIN-42221W500-D,, 5 FC-LIN-42221W500-E,, 6 FC-LIN-42221W500-F,, 7 FC-LIN-42221W500-G,, 8 FC-LIN-42221W500-H,, Name FC2G_445S004 FC2G_445S005 FC2G_445S006 FC2G_445S007 FC2G_445S008 FC2G_445S009 FC2G_445S010 FC2G_445S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 9.09 9.09 9.12 9.09 9.12 9.06 9.09 9.12 Area 93234 84855 100297 91532 94481 101933 110521 112180 Compound name: 13C2-PFDoA Response Factor: 4.0314 RRF SD: 0.770044, % Relative SD: 19.1012 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 22841 20301 23325 21380 18798 18995 16675 14160 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 10.125 10.368 10.666 10.620 12.468 13.312 16.441 19.651 %Rec 84.4 86.4 88.9 88.5 103.9 110.9 137.0 163.8 S/N 416 420 595 324 645 629 256 536 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MMX PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 188 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Compound name: 13C2-PFOUEA # ID Name Type RT 1 FC-LIN-42221W500-A,, FC2G_445S004 Standard 7.39 2 FC-LIN-42221W500-B,, FC2G_445S005 Standard 7.39 3 FC-LIN-42221W500-C,, FC2G_445S006 Standard 7.43 4 FC-LIN-42221W500-D,, FC2G_445S007 Standard 7.43 5 FC-LIN-42221W500-E,, FC2G_445S008 Standard 7.43 6 FC-LIN-42221W500-F,, FC2G_445S009 Standard 7.43 7 FC-LIN-42221W500-G,, FC2G_445S010 Standard 7.39 8 FC-LIN-42221W500-H,, FC2G_445S011 Standard 7.43 Compound name: 13C2-PFOUEA Response Factor: 1955.92 RRF SD: 309.787, % Relative SD: 15.8384 Response type: External Std, Area Curve type: Rf Area 22841 20301 23325 21380 18798 18995 16675 14160 IS Area Std. Conc 10.000 10.000 10.000 10.000 10.000 10.000 10.000 10.000 ng/L 11.678 10.379 11.925 10.931 9.611 9.711 8.525 7.240 %Rec 116.8 103.8 119.3 109.3 96.1 97.1 85.3 72.4 S/N 1298 2638 1273 1578 1184 0 1148 0 Acq.Date 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 13-Dec-12 Acq.Time 16:56:55 17:14:37 17:32:19 17:50:01 18:07:43 18:24:28 18:41:21 18:58:15 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 189 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time Method: G:\Masslynx\Master Methods\PFC\FC_060_10_01.mdb 03 Dec 2010 17:21:03 Calibration: 14 Dec 2012 11:01:10 ID: FC-LIN-42221W500-A,, Name: FC2G_445S004 Description: 1,,15uL Date: 13-Dec-2012 Time: 16:56:55 PFBA FC2G_445S004 5.13 100 1 .70e3 6.47 7.03 PFPeA FC2G_445S004 5.96 100 1 .9 5 e 3 9789 % PFHxA FC2G 445S004 min 5.00 6.00 7.00 min 5.00 6.00 7.00 PFHpA FC2G_445S004 6.74 100 3.18e3 12881 PFOA-1 FC2G 445S004 % :4.0 6 min min 5.00 6.00 7.00 PFOA-2 FC2G 445S004 min min PFNA FC2G 445S004 PFDA FC2G_445S004 _ 7.99 100 4.60e3 16544 PFUnA FC2G 445S004 % 12.02 min min 8.00 10.00 12.00 PFDoA FC2G_445S004 _ 9.09 100 4.34e3 19182 % PFBS-1 FC2G 445S004 min min 8.00 10.00 12.00 PFBS-2 FC2G_445S004 6.49 100 3.70e2 1549 % min min 5.00 6.00 7.00 PFHxS-1 FC2G 445S004 PFHxS-2 FC2G 445S004 min PFHxS-3 FC2G 445S004 100- 7.30 3.01e2 1183 PFOS-1 FC2G_445S004 8.23 100 7.63e2 2299 PFOS-2 FC2G_445S004 8.23 100 5.24e2 1715 PFOSA FC2G 445S004 %- % % 7.78 1 1 .4 9 1 2 .1 7 min 0 7.00 r-r min 8.00 0 8.00 10.00 min 12.00 8.00 10.00 min 12.00 PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 190 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-A,, Name: FC2G_445S004 Description: 1,,15uL Date: 13-Dec-2012 Time: 16:56:55 13C2-PFOA FC2G 445S004 100- 7.11 1.79e5 731049 %- 13C4-PFOA FC2G_445S004 7.11 100 6.22e4 ,257916 % 13C4-PFOS-1 FC2G 445S004 0 - ^ ^ 1 =rr min 7.00 8.00 0 7.00 8.00 min 13C4-PFBA FC2G 445S004 13C2-PFHxA FC2G 445S004 13C5-PFNA FC2G 445S004 13C4-PFOS-2 FC2G 445S004 min 13C2-PFDA FC2G 445S004 18O2-PFHxS-1 FC2G 445S004 18O2-PFHxS-2 FC2G 445S004 min 13C2-PFDoA FC2G 445S004 min 13C2-PFOUEA FC2G 445S004 min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 191 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-A,, Name: FC2G_445S004 Description: 1,,15uL Date: 13-Dec-2012 Time: 16:56:55 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.13 26 262.98 > 218.... 5.96 26 312.97> 268.98 6.37 26 362.97> 318.98 6.74 19 412.97> 368.98 7.11 19 412.97> 168.80 7.11 27 462.96 > 418.... 7.49 28 512.96 > 468.... 7.99 28 562.96 > 518.... 8.50 29 612.95 > 568.... 9.09 23 298.94> 79.96 6.49 24 298.94 > 98.95 6.49 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.30 23 398.84> 118.70 7.30 21 498.93> 79.96 8.23 22 498.93> 98.96 8.23 21 498 > 78 9.90 20 414.97 > 369.... 7.11 416.97 > 371.9 7.11 30 503 > 79.96 8.23 30 503 > 98.96 8.23 30 402.9>84 7.30 30 402.9>103 7.30 30 216.99>171.98 5.13 30 314.97>269.98 6.37 30 467.96>422.97 7.46 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.09 458.98 >393.98 7.39 Area 1702 1948 3763 3179 3480 659 2996 4595 3886 4341 944 370 843 351 301 763 524 542 178974 62157 10054 7580 9306 7048 29460 66835 61446 70921 93234 22841 IS Area 29460 66835 66835 66835 178974 178974 61446 70921 70921 93234 9306 7048 9306 7048 9306 10054 7580 10054 62157 22841 22841 22841 22841 22841 22841 22841 22841 22841 ng/L 0.567 0.611 0.553 0.600 0.565 0.500 0.571 0.426 0.528 0.463 1.027 1.114 1.021 1.144 1.163 1.110 1.033 0.613 34.055 14.953 17.734 18.052 18.904 20.097 12.158 12.812 11.869 11.315 10.125 11.678 LOD 0.1235 0.1432 0.0553 0.0671 0.0637 0.3039 0.3157 0.1922 0.0746 0.1321 0.4630 0.1519 0.1535 0.0147 0.0076 0.0453 0.0839 0.0270 %Rec 113.5 122.3 110.6 119.9 113.0 100.0 114.3 85.2 105.6 92.6 102.7 111.4 102.1 114.4 116.3 111.0 103.3 122.5 94.6 124.6 98.5 100.3 105.0 111.7 101.3 106.8 98.9 94.3 84.4 116.8 S/N 30 86 85 56 85 50 31 32 60 49 177 32 228 34 75 23 17 68 1348 0 400 110 409 0 196 3315 4801 678 416 1298 Noise 203 114 211 230 159 46 399 511 270 391 27 48 12 46 16 102 101 32 542 0 99 256 99 0 490 101 52 375 1018 70 Height 6057 9789 17937 12881 13536 2309 12456 16544 16158 19182 4755 1549 2767 1559 1183 2299 1715 2163 731049 257916 39456 28278 40323 28046 96083 334313 249216 254000 423361 90284 IS Height 96083 334313 334313 334313 731049 731049 249216 254000 254000 423361 40323 28046 40323 28046 40323 39456 28278 39456 257916 90284 90284 90284 90284 90284 90284 90284 90284 90284 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 192 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-B,, Name: FC2G_445S005 Description: 1,,15uL Date: 13-Dec-2012 Time: 17:14:37 PFBA FC2G_445S005 5.16 100 3.26e3 13201 6.45 7.08 min 5.00 6.00 7.00 PFPeA FC2G 445S005 PFNA FC2G 445S005 PFDA FC2G 445S005 PFHxA FC2G 445S005 min PFUnA FC2G 445S005 PFHpA FC2G 445S005 min PFDoA FC2G 445S005 PFHxS-1 FC2G 445S005 min PFHxS-2 FC2G 445S005 min PFHxS-3 FC2G 445S005 min PFOS-1 FC2G 445S005 min min min PFOA-1 FC2G 445S005 PFOA-2 FC2G 445S005 min min PFBS-1 FC2G_445S005 100 6.47 1.77e3 9224 %- PFBS-2 FC2G 445S005 min min 5.00 6.00 7.00 PFOS-2 FC2G_445S005 8.23 100 9.48e2 3407 % PFOSA FC2G 445S005 8.00 10.00 min 12.00 min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 193 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-B,, Name: FC2G_445S005 Description: 1,,15uL Date: 13-Dec-2012 Time: 17:14:37 13C2-PFOA FC2G 445S005 13C4-PFOA FC2G 445S005 13C4-PFOS-1 FC2G 445S005 13C4-PFOS-2 FC2G 445S005 18O2-PFHxS-1 FC2G 445S005 18O2-PFHxS-2 FC2G 445S005 13C4-PFBA FC2G 445S005 min 13C2-PFHxA FC2G 445S005 min 13C5-PFNA FC2G 445S005 min 13C2-PFDA FC2G 445S005 min 13C2-PFDoA FC2G 445S005 min 13C2-PFOUEA FC2G 445S005 min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 194 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-B,, Name: FC2G_445S005 Description: 1,,15uL Date: 13-Dec-2012 Time: 17:14:37 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.16 26 262.98 > 218.... 5.96 26 312.97> 268.98 6.36 26 362.97> 318.98 6.74 19 412.97> 368.98 7.11 19 412.97> 168.80 7.11 27 462.96 > 418.... 7.46 28 512.96 > 468.... 7.93 28 562.96 > 518.... 8.50 29 612.95 > 568.... 9.09 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.49 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.25 23 398.84> 118.70 7.30 21 498.93> 79.96 8.23 22 498.93> 98.96 8.23 21 498 > 78 9.87 20 414.97 > 369.... 7.11 416.97 > 371.9 7.11 30 503 > 79.96 8.23 30 503 > 98.96 8.23 30 402.9>84 7.30 30 402.9>103 7.30 30 216.99>171.98 5.14 30 314.97>269.98 6.36 30 467.96>422.97 7.46 30 514.96 >469.96 7.93 30 614.95 > 569.... 9.09 458.98 >393.98 7.39 Area 3260 4081 7458 6544 6169 1818 6297 9683 9165 9683 1769 922 1763 883 530 1662 948 1454 163290 54677 10200 6871 8155 6816 25526 60698 64296 65473 84855 20301 IS Area 25526 60698 60698 60698 163290 163290 64296 65473 65473 84855 8155 6816 8155 6816 8155 10200 6871 10200 54677 20301 20301 20301 20301 20301 20301 20301 20301 20301 ng/L 1.371 1.272 1.286 1.204 1.179 1.450 1.136 1.370 1.294 1.340 2.589 2.529 2.923 2.617 2.635 2.600 2.468 1.336 35.321 13.153 20.243 18.408 18.638 21.866 11.852 13.091 13.972 11.753 10.368 10.379 LOD 0.0240 0.1450 0.1580 0.0377 0.0737 0.0240 0.0876 0.2643 0.2250 0.0567 0.1829 0.1418 0.1125 0.2232 0.0653 0.1583 0.1162 0.0153 0.0090 0.0377 0.0825 0.0118 %Rec 109.7 101.7 102.9 96.3 94.3 116.0 90.9 109.6 103.6 107.2 103.6 101.2 116.9 104.7 105.4 104.0 98.7 106.9 98.1 109.6 112.5 102.3 103.5 121.5 98.8 109.1 116.4 97.9 86.4 103.8 S/N 40 92 211 77 32 96 258 133 88 126 199 136 0 0 520 23 39 278 0 278 499 234 822 423 284 3192 5026 870 420 2638 Noise 332 227 180 355 830 75 98 274 475 347 46 26 0 0 4 181 88 16 0 785 76 111 38 66 334 94 55 280 902 31 Height 13201 20930 37907 27371 26944 7193 25288 36593 41784 43782 9224 3537 6416 3276 2036 4217 3407 4512 673331 218379 37779 25998 31376 27830 94930 301384 278797 243932 378629 81277 IS Height 94930 301384 301384 301384 673331 673331 278797 243932 243932 378629 31376 27830 31376 27830 31376 37779 25998 37779 218379 81277 81277 81277 81277 81277 81277 81277 81277 81277 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 195 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-C,, Name: FC2G_445S006 Description: 1,,15uL Date: 13-Dec-2012 Time: 17:32:19 PFBA FC2G 445S006 PFPeA FC2G 445S006 PFHxA FC2G 445S006 PFHpA FC2G 445S006 PFOA-1 FC2G 445S006 PFOA-2 FC2G 445S006 PFNA FC2G 445S006 min PFDA FC2G 445S006 min PFUnA FC2G 445S006 min PFDoA FC2G 445S006 min PFBS-1 FC2G 445S006 min PFBS-2 FC2G 445S006 min PFHxS-1 FC2G 445S006 min PFHxS-2 FC2G 445S006 min PFHxS-3 FC2G 445S006 min PFOS-1 FC2G 445S006 min PFOS-2 FC2G 445S006 min PFOSA FC2G 445S006 min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 196 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-C,, Name: FC2G_445S006 Description: 1,,15uL Date: 13-Dec-2012 Time: 17:32:19 13C2-PFOA FC2G 445S006 13C4-PFOA FC2G 445S006 13C4-PFBA FC2G 445S006 min 13C2-PFHxA FC2G 445S006 13C4-PFOS-1 FC2G 445S006 min 13C5-PFNA FC2G 445S006 13C4-PFOS-2 18O2-PFHxS-1 18O2-PFHxS-2 FC2G 445S006 FC2G 445S006 FC2G_445S006 8.29 J100%- 7.46e3 27410 100%- 7.30 9.97e3 41115 100q %- 7.30 6.82e3 26464 min 0 - m in 0 / V, -T min 0- 8.00 10.00 12.00 7.00 8.00 7.00 8.00 min 13C2-PFDA FC2G 445S006 13C2-PFDoA FC2G 445S006 13C2-PFOUEA FC2G 445S006 min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 197 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-C,, Name: FC2G_445S006 Description: 1,,15uL Date: 13-Dec-2012 Time: 17:32:19 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.14 26 262.98 > 218.... 5.98 26 312.97> 268.98 6.37 26 362.97> 318.98 6.74 19 412.97> 368.98 7.11 19 412.97> 168.80 7.11 27 462.96 > 418.... 7.49 28 512.96 > 468.... 7.99 28 562.96 > 518.... 8.53 29 612.95 > 568.... 9.12 23 298.94> 79.96 6.49 24 298.94 > 98.95 6.49 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.30 23 398.84> 118.70 7.30 21 498.93> 79.96 8.29 22 498.93> 98.96 8.23 21 498 > 78 9.90 20 414.97 > 369.... 7.11 416.97 > 371.9 7.11 30 503 > 79.96 8.29 30 503 > 98.96 8.29 30 402.9>84 7.30 30 402.9>103 7.30 30 216.99>171.98 5.14 30 314.97>269.98 6.36 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.12 458.98 >393.98 7.43 Area 11699 15995 27050 28341 25268 6596 26363 30905 35480 38200 6729 3517 5780 3235 1815 5671 3809 5000 176604 61363 10723 7462 9974 6816 29193 64980 62761 67478 100297 23325 IS Area 29193 64980 64980 64980 176604 176604 62761 67478 67478 100297 9974 6816 9974 6816 9974 10723 7462 10723 61363 23325 23325 23325 23325 23325 23325 23325 23325 23325 ng/L 4.508 4.375 4.521 4.498 4.707 4.793 4.832 4.896 4.770 4.808 8.782 9.035 8.425 8.991 7.926 8.861 10.226 3.974 34.039 14.762 18.522 17.402 19.840 19.031 11.797 12.197 11.871 10.543 10.666 11.925 LOD 0.1862 0.1455 0.1435 0.0310 0.0256 0.0777 0.2961 0.2250 0.8740 0.2199 0.1295 0.1200 0.1603 0.1411 0.0084 0.0142 0.0484 0.0575 0.0281 %Rec 90.2 87.5 90.4 90.0 94.1 95.9 96.6 97.9 95.4 96.2 87.8 90.4 84.2 89.9 79.3 88.6 102.3 79.5 94.6 123.0 102.9 96.7 110.2 105.7 98.3 101.6 98.9 87.9 88.9 119.3 S/N 49 325 611 548 0 0 1068 286 384 428 928 416 0 0 205 18 118 192 0 342 438 297 0 0 213 5218 2521 601 595 1273 Noise 821 240 221 219 0 0 101 385 396 397 35 41 0 0 35 846 107 79 0 726 93 92 0 0 469 60 101 417 727 74 Height 40612 78143 135112 119980 103440 26117 107814 110308 151846 169630 32067 17121 20790 12445 7179 15630 12587 15218 733596 248304 40862 27410 41115 26464 100060 314460 253833 250344 432776 94053 IS Height 100060 314460 314460 314460 733596 733596 253833 250344 250344 432776 41115 26464 41115 26464 41115 40862 27410 40862 248304 94053 94053 94053 94053 94053 94053 94053 94053 94053 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 198 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-D,, Name: FC2G_445S007 Description: 1,,15uL Date: 13-Dec-2012 Time: 17:50:01 PFBA FC2G 445S007 PFPeA FC2G 445S007 PFHxA FC2G 445S007 PFHpA FC2G 445S007 PFOA-1 FC2G 445S007 PFOA-2 FC2G 445S007 min min min min min PFNA FC2G 445S007 PFDA FC2G 445S007 PFUnA FC2G 445S007 PFDoA FC2G_445S007 100- 9.09 1 .4 4 e 5 659398 % PFBS-1 FC2G 445S007 PFBS-2 FC2G 445S007 PFHxS-1 FC2G 100- 445S007 7.30 2.19e4 81274 %- min PFHxS-2 FC2G 100- 445S007 7.30 1.27e4 49203 %- min PFHxS-3 FC2G 445S007 100- 7.30 7.69e3 29594 %- min 0 min 8.00 10.00 12.00 PFOS-1 FC2G 100- 445S007 8.23 2.06e4 54010 %- PFOS-2 FC2G_445S007 100^ 8.23 1 .42e4 46891 %: min PFOSA FC2G 445S007 07.00 =f min 8.00 07.00 =r min 8.00 07.00 r=f min 8.00 V 0- 8.00 9.93 10.00 m in 12.00 0^ 8.00 10.00 min 12.00 min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 199 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-D,, Name: FC2G_445S007 Description: 1,,15uL Date: 13-Dec-2012 Time: 17:50:01 13C2-PFOA FC2G 445S007 100- 7.11 1 .6 4 e 5 655272 %- 13C4-PFOA FC2G_445S007 7.11 100 5.67e4 1215664 % 13C4-PFOS-1 FC2G 445S007 07.00 13C4-PFBA FC2G 445S007 =rr min 8.00 0 7.00 13C2-PFHxA FC2G 445S007 8.00 min 13C5-PFNA FC2G 445S007 13C4-PFOS-2 FC2G 445S007 min 13C2-PFDA FC2G 445S007 18O2-PFHxS-1 FC2G 445S007 18O2-PFHxS-2 FC2G 445S007 min 13C2-PFDoA FC2G 445S007 min 13C2-PFOUEA FC2G 445S007 min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 200 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-D,, Name: FC2G_445S007 Description: 1,,15uL Date: 13-Dec-2012 Time: 17:50:01 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.18 26 262.98 > 218.... 5.98 26 312.97> 268.98 6.37 26 362.97> 318.98 6.74 19 412.97> 368.98 7.11 19 412.97> 168.80 7.11 27 462.96 > 418.... 7.49 28 512.96 > 468.... 7.99 28 562.96 > 518.... 8.53 29 612.95 > 568.... 9.09 23 298.94> 79.96 6.49 24 298.94 > 98.95 6.49 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.30 23 398.84> 118.70 7.30 21 498.93> 79.96 8.23 22 498.93> 98.96 8.23 21 498 > 78 9.90 20 414.97 > 369.... 7.11 416.97 > 371.9 7.11 30 503 > 79.96 8.23 30 503 > 98.96 8.29 30 402.9>84 7.30 30 402.9>103 7.30 30 216.99>171.98 5.18 30 314.97>269.98 6.36 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.09 458.98 >393.98 7.43 Area 42289 60142 100408 104254 94311 22890 95907 115554 126694 144155 26283 13781 21905 12711 7686 20621 14211 21170 164434 56742 9486 8114 9157 6371 27354 58266 57763 62623 91532 21380 IS Area 27354 58266 58266 58266 164434 164434 57763 62623 62623 91532 9157 6371 9157 6371 9157 9486 8114 9486 56742 21380 21380 21380 21380 21380 21380 21380 21380 21380 ng/L 17.665 18.014 18.962 18.089 19.151 17.796 19.055 20.743 18.316 20.433 38.475 37.200 35.862 37.095 37.658 36.986 35.976 18.251 34.274 13.650 17.876 20.643 19.872 19.406 12.060 11.932 11.920 10.674 10.620 10.931 LOD 0.5435 0.1739 0.1626 0.0310 0.0340 0.0822 0.2635 0.2250 0.0640 0.2051 0.0515 0.1004 0.1488 0.0676 0.0978 0.1281 0.0284 0.0080 0.0529 0.1137 0.0208 %Rec 88.3 90.1 94.8 90.4 95.8 89.0 95.3 103.7 91.6 102.2 96.2 93.0 89.7 92.7 94.1 92.5 89.9 91.3 95.2 113.8 99.3 114.7 110.4 107.8 100.5 99.4 99.3 89.0 88.5 109.3 S/N 90 742 3750 1098 1407 0 2531 1048 1322 1897 6178 2829 0 0 0 320 338 1567 2093 0 506 390 943 619 242 1610 4915 594 324 1578 Noise 1662 372 128 379 262 0 154 404 410 348 20 23 0 0 0 169 139 46 313 0 68 74 40 41 370 176 49 395 1247 52 Height 149855 275802 481227 416250 368520 91209 389749 423187 541926 659398 125309 65986 81274 49203 29594 54010 46891 71784 655272 215664 34283 29041 37377 25317 89509 284126 241058 234751 403706 81606 IS Height 89509 284126 284126 284126 655272 655272 241058 234751 234751 403706 37377 25317 37377 25317 37377 34283 29041 34283 215664 81606 81606 81606 81606 81606 81606 81606 81606 81606 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 201 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-E,, Name: FC2G_445S008 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:07:43 PFBA FC2G 445S008 100- 5.11 2.14e5 693898 %- PFPeA FC2G_445S008 5.96 _ 100 2.94e5 1375798 % PFHxA FC2G 445S008 0 - li -m min 5.00 6.00 7.00 0 min 5.00 6.00 7.00 PFNA FC2G 445S008 7.49 1004.72e5 1888519 %- PFDA FC2G_445S008 7.99 100 5.52e5 2030209 % PFUnA FC2G 445S008 07.00 PFHxS-1 FC2G 445S008 8.00 min 0 8.00 111111111 1111 min 10.00 12.00 PFHxS-2 FC2G 445S008 PFHxS-3 FC2G 445S008 PFHpA FC2G 445S008 min PFDoA FC2G 445S008 min PFOS-1 FC2G 445S008 PFOA-1 FC2G 445S008 min PFBS-1 FC2G 445S008 min PFOS-2 FC2G 445S008 PFOA-2 FC2G 445S008 min PFBS-2 FC2G 445S008 min PFOSA FC2G 445S008 min min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 202 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-E,, Name: FC2G_445S008 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:07:43 13C2-PFOA FC2G 445S008 13C4-PFOA FC2G 445S008 13C4-PFOS-1 FC2G 445S008 13C4-PFOS-2 FC2G 445S008 18O2-PFHxS-1 FC2G 445S008 18O2-PFHxS-2 FC2G 445S008 13C4-PFBA FC2G 445S008 min 13C2-PFHxA FC2G 445S008 min 13C5-PFNA FC2G 445S008 min 13C2-PFDA FC2G 445S008 min 13C2-PFDoA FC2G 445S008 min 13C2-PFOUEA FC2G 445S008 min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 203 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-E,, Name: FC2G_445S008 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:07:43 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.11 26 262.98 > 218.... 5.96 26 312.97> 268.98 6.36 26 362.97> 318.98 6.74 19 412.97> 368.98 7.11 19 412.97> 168.80 7.11 27 462.96 > 418.... 7.49 28 512.96 > 468.... 7.99 28 562.96 > 518.... 8.53 29 612.95 > 568.... 9.12 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.30 23 398.84> 118.70 7.30 21 498.93> 79.96 8.23 22 498.93> 98.96 8.29 21 498 > 78 9.93 20 414.97 > 369.... 7.11 416.97 > 371.9 7.11 30 503 > 79.96 8.23 30 503 > 98.96 8.23 30 402.9>84 7.30 30 402.9>103 7.30 30 216.99>171.98 5.13 30 314.97>269.98 6.36 30 467.96>422.97 7.46 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.12 458.98 >393.98 7.43 Area 213591 293936 472991 530786 452340 113491 471902 552400 671038 706897 125842 66054 106920 57268 34687 110728 72388 112866 147281 47889 9214 6988 7654 5206 24645 53679 52387 63392 94481 18798 IS Area 24645 53679 53679 53679 147281 147281 52387 63392 63392 94481 7654 5206 7654 5206 7654 9214 6988 9214 47889 18798 18798 18798 18798 18798 18798 18798 18798 18798 ng/L 99.497 95.340 98.317 99.883 103.692 99.005 103.082 101.028 97.651 100.284 221.475 217.479 210.774 203.944 205.297 204.433 210.588 96.015 36.374 11.520 19.747 20.221 18.891 18.038 12.358 12.503 12.295 12.289 12.468 9.611 LOD 0.0620 0.1293 0.1420 0.0310 0.0120 0.1465 0.3144 0.2803 0.2991 0.2661 0.3180 0.1003 0.2587 0.2160 0.2118 0.0168 0.0163 0.0708 0.0630 0.0243 %Rec 99.5 95.3 98.3 99.9 103.7 99.0 103.1 101.0 97.7 100.3 110.7 108.7 105.4 102.0 102.6 102.2 105.3 96.0 101.0 96.0 109.7 112.3 105.0 100.2 103.0 104.2 102.5 102.4 103.9 96.1 S/N 2015 10920 10164 11928 0 0 0 1346 2940 8277 15154 7804 0 10638 0 936 856 1898 0 0 576 226 0 251 140 3043 2428 502 645 1184 Noise 344 126 241 180 0 0 0 1508 961 343 37 38 0 20 0 325 277 203 0 0 59 114 0 79 536 92 88 464 606 60 Height 693898 1375798 2444874 2149474 1887327 464435 1888519 2030209 2825081 2839802 555471 296430 397302 210529 126405 304510 236747 385730 622565 200398 34203 25644 30217 19848 74871 278673 214225 232629 390455 71587 IS Height 74871 278673 278673 278673 622565 622565 214225 232629 232629 390455 30217 19848 30217 19848 30217 34203 25644 34203 200398 71587 71587 71587 71587 71587 71587 71587 71587 71587 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 204 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-F,, Name: FC2G_445S009 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:24:28 PFBA FC2G_445S009 100n 5.14 4.21e5 1329855 % PFPeA FC2G 445S009 100- 5.98 5.66e5 1 2418352 %- PFHxA FC2G_445S009 6.36 100 8.89e5 4279719 % PFHpA FC2G 445S009 0 n min 5.00 6.00 7.00 'I0 - ..................... min 5.00 6.00 7.00 0 min 5.00 6.00 7.00 PFNA FC2G 445S009 PFDA FC2G 445S009 PFUnA FC2G 445S009 PFDoA FC2G 445S009 PFOA-1 FC2G 445S009 min PFBS-1 FC2G 445S009 PFOA-2 FC2G 445S009 min PFBS-2 FC2G 445S009 min PFHxS-1 FC2G 445S009 min PFHxS-2 FC2G 445S009 min PFHxS-3 FC2G 445S009 min PFOS-1 FC2G 445S009 min PFOS-2 FC2G 445S009 min PFOSA FC2G 445S009 min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 205 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-F,, Name: FC2G_445S009 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:24:28 13C2-PFOA FC2G 445S009 13C4-PFOA FC2G 445S009 13C4-PFOS-1 FC2G 445S009 13C4-PFOS-2 FC2G 445S009 18O2-PFHxS-1 FC2G 445S009 18O2-PFHxS-2 FC2G 445S009 13C4-PFBA FC2G 445S009 min 13C2-PFHxA FC2G 445S009 min 13C5-PFNA FC2G 445S009 min 13C2-PFDA FC2G 445S009 min 13C2-PFDoA FC2G 445S009 min 13C2-PFOUEA FC2G 445S009 min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 206 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-F,, Name: FC2G_445S009 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:24:28 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.14 26 262.98 > 218.... 5.98 26 312.97> 268.98 6.36 26 362.97> 318.98 6.74 19 412.97> 368.98 7.11 19 412.97> 168.80 7.11 27 462.96 > 418.... 7.49 28 512.96 > 468.... 7.99 28 562.96 > 518.... 8.50 29 612.95 > 568.... 9.09 23 298.94> 79.96 6.49 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.30 23 398.84> 118.70 7.30 21 498.93> 79.96 8.23 22 498.93> 98.96 8.23 21 498 > 78 9.90 20 414.97 > 369.... 7.11 416.97 > 371.9 7.11 30 503 > 79.96 8.23 30 503 > 98.96 8.23 30 402.9>84 7.30 30 402.9>103 7.30 30 216.99>171.98 5.14 30 314.97>269.98 6.36 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.06 458.98 >393.98 7.43 Area 421406 566115 888540 1004495 853031 221628 917096 1032872 1344974 1522557 219038 115161 198806 104543 65881 201138 133090 229818 145888 45943 8227 6470 7157 5242 25200 48323 51976 57749 101933 18995 IS Area 25200 48323 48323 48323 145888 145888 51976 57749 57749 101933 7157 5242 7157 5242 7157 8227 6470 8227 45943 18995 18995 18995 18995 18995 18995 18995 18995 18995 ng/L 192.121 204.560 208.462 211.217 199.162 196.690 201.344 213.460 221.955 208.066 411.492 376.960 418.652 370.394 418.995 413.788 409.504 208.426 37.556 11.052 17.451 18.527 17.481 17.974 12.505 11.138 12.072 11.079 13.312 9.711 LOD 0.1639 0.1506 0.1595 0.0310 0.0120 0.1384 0.2684 0.3685 0.3945 0.4362 0.3113 0.1783 0.2510 0.3856 0.0193 0.0087 0.0848 0.0674 %Rec 96.1 102.3 104.2 105.6 99.6 98.3 100.7 106.7 111.0 104.0 102.9 94.2 104.7 92.6 104.7 103.4 102.4 104.2 104.3 92.1 96.9 102.9 97.1 99.9 104.2 92.8 100.6 92.3 110.9 97.1 S/N 2159 11971 21372 13584 0 0 0 4803 7104 12111 32828 23419 0 7432 0 1500 1370 4612 0 0 277 201 0 0 82 2177 4295 368 629 0 Noise 616 202 200 288 0 0 0 787 801 535 28 20 0 51 0 340 306 170 0 0 108 113 0 0 1000 108 48 569 664 0 Height 1329855 2418352 4279719 3914694 3549623 915353 3613896 3780743 5692922 6477421 934487 467050 702437 377231 235487 510564 419479 783225 603779 188669 29955 22679 26502 19603 81556 234401 206700 209295 417578 73343 IS Height 81556 234401 234401 234401 603779 603779 206700 209295 209295 417578 26502 19603 26502 19603 26502 29955 22679 29955 188669 73343 73343 73343 73343 73343 73343 73343 73343 73343 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 207 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-G,, Name: FC2G_445S010 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:41:21 PFBA FC2G 445S010 100- 5.11 9.54e5 2819195 %- l/ 0- n min 5.00 6.00 7.00 PFPeA FC2G 445S010 100- 5.95 1.25e6 5486431 %- 1 0- _ nl m in 5.00 6.00 7.00 PFHxA FC2G 445S010 100- 6.32 1.81e6 9542781 %- ,1 0 min 5.00 6.00 7.00 PFHpA FC2G 445S010 100- 6.69 2.06e6 1 8084141 %- 0 min 5.00 6.00 7.00 PFOA-1 FC2G_445S010 100q 7.06 1 .78e6 7387648 %- 0-- 7.00 8.00 min PFOA-2 FC2G 445S010 min PFNA FC2G 445S010 PFDA FC2G 445S010 min PFUnA FC2G 100- 445S010 8.50 2.82e6 10940871 PFDoA FC2G 100- 445S010 9.09 3.46e6 13828879 PFBS-1 FC2G_445S010 6.45 100 4.39e5 1805813 PFBS-2 FC2G 445S010 %- %- % L kJ rmin 0 - ................... min 0- ................. t min 0 min 8.00 10.00 12.00 8.00 10.00 12.00 5.00 6.00 7.00 min PFHxS-1 FC2G 100- %- 445S010 7.30 3.95e5 1306717 \ 07.00 8.00 min PFHxS-2 FC2G 100- 445S010 7.25 2.14e5 742338 %- 07.00 =f min 8.00 PFHxS-3 FC2G 445S010 100- 7.25 1 .3 0 e 5 434415 %- / 0- 7.00 i r=f min 8.00 PFOS-1 FC2G 100- 445S010 8.23 i" 4.40e5 1144079 %- V, 0- 8.00 9.90 10.00 min 12.00 PFOS-2 FC2G 445S010 100- 8.23 2.81e5 18 3 1 8 3 8 %- Jk 0- 8.00 10.00 m in 12.00 PFOSA FC2G 445S010 9.90 100 5.61e5 1825826 % 0 8.00 10.00 min 12.00 PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 208 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-G,, Name: FC2G_445S010 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:41:21 13C2-PFOA FC2G 445S010 13C4-PFOA FC2G 445S010 13C4-PFOS-1 FC2G 445S010 13C4-PFOS-2 FC2G 445S010 18O2-PFHxS-1 FC2G 445S010 18O2-PFHxS-2 FC2G 445S010 13C4-PFBA FC2G 445S010 min 13C2-PFHxA FC2G 445S010 min 13C5-PFNA FC2G 445S010 min 13C2-PFDA FC2G 445S010 min 13C2-PFDoA FC2G 445S010 min 13C2-PFOUEA FC2G 445S010 min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 209 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-G,, Name: FC2G_445S010 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:41:21 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.11 26 262.98 > 218.... 5.95 26 312.97> 268.98 6.32 26 362.97> 318.98 6.69 19 412.97> 368.98 7.06 19 412.97> 168.80 7.06 27 462.96 > 418.... 7.46 28 512.96 > 468.... 7.93 28 562.96 > 518.... 8.50 29 612.95 > 568.... 9.09 23 298.94> 79.96 6.45 24 298.94 > 98.95 6.43 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.25 23 398.84> 118.70 7.25 21 498.93> 79.96 8.23 22 498.93> 98.96 8.23 21 498 > 78 9.90 20 414.97 > 369.... 7.06 416.97 > 371.9 7.06 30 503 > 79.96 8.23 30 503 > 98.96 8.23 30 402.9>84 7.30 30 402.9>103 7.25 30 216.99>171.98 5.07 30 314.97>269.98 6.32 30 467.96>422.97 7.46 30 514.96 >469.96 7.93 30 614.95 > 569.... 9.09 458.98 >393.98 7.39 Area 953630 1246496 1813293 2055822 1776266 480485 1926313 2264803 2816578 3461389 439326 225115 395234 213611 129524 440275 280510 560574 125019 40127 7488 5370 6135 3840 21251 43496 44006 60934 110521 16675 IS Area 21251 43496 43496 43496 125019 125019 44006 60934 60934 110521 6135 3840 6135 3840 6135 7488 5370 7488 40127 16675 16675 16675 16675 16675 16675 16675 16675 16675 ng/L 516.166 505.025 492.664 488.032 497.215 510.440 495.377 474.551 472.106 484.187 956.132 1011.489 966.396 1042.150 971.484 980.906 979.408 497.907 36.848 9.653 18.093 17.518 17.070 14.997 12.013 11.421 11.643 13.317 16.441 8.525 LOD 0.3272 0.1367 0.1715 0.0310 0.0120 0.1584 0.3104 0.2250 0.8537 1.0257 0.4177 0.1748 0.1684 0.0237 0.3608 0.0231 0.0451 0.0968 0.1915 0.0223 %Rec 103.2 101.0 98.5 97.6 99.4 102.1 99.1 94.9 94.4 96.8 95.6 101.1 96.6 104.2 97.1 98.1 97.9 99.6 102.4 80.4 100.5 97.3 94.8 83.3 100.1 95.2 97.0 111.0 137.0 85.3 S/N 3615 41155 61073 22109 0 0 0 5790 10581 10729 44106 34911 0 0 0 2000 1853 6474 0 0 293 285 1927 0 75 1922 787 384 256 1148 Noise 780 133 156 366 0 0 0 1405 1034 1289 41 26 0 0 0 572 449 282 0 0 96 69 11 0 849 117 227 589 1712 58 Height IS Height 2819195 63446 5486431 224421 9542781 224421 8084141 224421 7387648 538275 2032274 538275 7777143 178490 8136139 226144 10940871 226144 13828879 438419 1805813 21861 902602 14088 1306717 21861 742338 14088 434415 21861 1144079 28164 831838 19581 1825826 28164 538275 168417 168417 28164 66524 19581 66524 21861 66524 14088 66524 63446 66524 224421 66524 178490 66524 226144 66524 438419 66524 66524 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 210 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-H,, Name: FC2G_445S011 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:58:15 PFBA FC2G 445S011 PFPeA FC2G 445S011 PFHxA FC2G 445S011 PFHpA FC2G 445S011 PFOA-1 FC2G 445S011 PFOA-2 FC2G 445S011 PFNA FC2G 445S011 min PFDA FC2G 445S011 min PFUnA FC2G 445S011 min PFDoA FC2G 445S011 min PFBS-1 FC2G 445S011 min PFBS-2 FC2G 445S011 min PFHxS-1 FC2G 445S011 min PFHxS-2 FC2G 445S011 min PFHxS-3 FC2G 445S011 min PFOS-1 FC2G 445S011 min PFOS-2 FC2G 445S011 min PFOSA FC2G 445S011 min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 211 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-H,, Name: FC2G_445S011 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:58:15 13C2-PFOA FC2G 445S011 13C4-PFOA FC2G 445S011 13C4-PFOS-1 FC2G 445S011 13C4-PFOS-2 FC2G 445S011 18O2-PFHxS-1 FC2G 445S011 18O2-PFHxS-2 FC2G 445S011 13C4-PFBA FC2G 445S011 min 13C2-PFHxA FC2G 445S011 min 13C5-PFNA FC2G 445S011 13C2-PFDA FC2G 445S011 min 13C2-PFDoA FC2G 445S011 min 13C2-PFOUEA FC2G 445S011 min min min min min min min PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 212 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC2G_445-A1 .qld Last Altered: Friday, December 14, 2012 11:01:10 Pacific Standard Time Printed: Sunday, December 16, 2012 09:33:39 Pacific Standard Time ID: FC-LIN-42221W500-H,, Name: FC2G_445S011 Description: 1,,15uL Date: 13-Dec-2012 Time: 18:58:15 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.20 26 262.98 > 218.... 5.98 26 312.97> 268.98 6.36 26 362.97> 318.98 6.74 19 412.97> 368.98 7.11 19 412.97> 168.80 7.11 27 462.96 > 418.... 7.49 28 512.96 > 468.... 7.99 28 562.96 > 518.... 8.56 29 612.95 > 568.... 9.12 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.30 23 398.84> 118.70 7.30 21 498.93> 79.96 8.23 22 498.93> 98.96 8.29 21 498 > 78 9.93 20 414.97 > 369.... 7.11 416.97 > 371.9 7.11 30 503 > 79.96 8.29 30 503 > 98.96 8.29 30 402.9>84 7.30 30 402.9>103 7.30 30 216.99>171.98 5.16 30 314.97>269.98 6.36 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.12 458.98 >393.98 7.43 Area 1826622 2438345 3315686 4086324 3356814 888554 3754168 4575060 5587121 6343367 780518 390955 689052 356435 214852 786051 532049 1006244 100831 30166 5039 3443 4060 2738 16912 35274 33246 58399 112180 14160 IS Area 16912 35274 35274 35274 100831 100831 33246 58399 58399 112180 4060 2738 4060 2738 4060 5039 3443 5039 30166 14160 14160 14160 14160 14160 14160 14160 14160 14160 ng/L 1244.856 1247.545 1252.147 1253.279 1251.114 1245.993 1251.356 1264.185 1265.057 1269.169 2513.525 2497.700 2509.441 2487.173 2508.404 2504.839 2504.844 1088.040 39.533 7.257 14.336 13.227 13.304 12.590 11.257 10.906 10.358 15.029 19.651 7.240 LOD 0.2164 0.1830 0.0019 0.1523 0.0310 0.0120 0.0804 0.1920 0.3995 0.2250 1.9786 3.6719 1.0640 0.1999 0.3882 0.4125 0.0157 0.0824 0.1160 %Rec 99.6 99.8 100.2 100.3 100.1 99.7 100.1 101.1 101.2 101.5 100.5 99.9 100.4 99.5 100.3 100.2 100.2 87.0 109.8 60.5 79.6 73.5 73.9 69.9 93.8 90.9 86.3 125.2 163.8 72.4 S/N 12024 36887 44296 87183 0 0 0 6641 17560 16794 61314 45765 0 0 0 2485 1977 4989 0 0 195 91 0 0 62 2803 0 531 536 0 Noise 440 268 377 179 0 0 0 2473 1256 1446 46 31 0 0 0 733 766 632 0 0 89 128 0 0 787 64 0 406 841 0 Height IS Height 5294785 48508 9870934 180233 16680690 180233 15587718 180233 13981256 420804 3715083 420804 13590439 122614 16424977 215978 22063054 215978 24287154 451307 2815345 13847 1421163 8691 2159335 13847 1151307 8691 675435 13847 1821830 17369 1513933 11737 3154100 17369 420804 122551 122551 17369 53946 11737 53946 13847 53946 8691 53946 48508 53946 180233 53946 122614 53946 215978 53946 451307 53946 53946 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MMX 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMX 1.0 MM PV by LIU 14-Dec-12, SV by AP 16-Dec-12. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 213 of 307 www.axysanalytical.com _______________ m i __________ppc- \ _____________________________ Sam ple List Report M assLynx 4.1 S C N 627 Sample List: G:\Masslynx\lnst_G\_Master.PRO\SampleDB\FC2G_455.SPL Last Modified: Tuesday, December 18, 2012 16:01:48 Pacific Standard Time Printed: Tuesday, December 18, 2012 16:07:45 Pacific Standard Time Page 1 of 1 Page Position (1,1) File Name Sample ID File Text Bottle Signln SignDut Inj VoL 1 FC2G 455S001 MeOH,, 1,,15uL ' 1:8,A 2 FC2G 455S002 MeOH,, T,,15uL 1:8,A 3 FC2G 455S003 FC-LIN-42221W500-A,, 1,,15uL 1:1,A 4 FC2G 455S004 FC-LIN-42221W500-D,, 1,,15uL 1:1,D 5 FC2G 455S005 FC-LIN-42221W500-D,, 1,,15uL 1:1,D 6 FC2G 455S006 WG42273-104,,SAR 1,WG42273,15/4000uL 3:2,B 7 FC2G 455S007 WG42273-102,,SPM 1,WG42273,15/4000uL 3:2,C 8 FC2G 455S008 MeOH,, 1,,15uL . 1:8,A 9 FC2G 455S009 MeOH,, 1,,15uL - 1:8,A 10 FC2G 455S010 WG42273-101 ,,BLK 1,WG42273,15/4000uL 3:2,D 11 FC2G 455S011 L18989-1,, 1,WG42273,15/4000uL 3:2,E 12 FC2G 455S012 L18989-2,, 1,WG42273,15/4000uL 3:2,F 13 FC2G 455S013 L18989-3,, 1,WG42273,15/4000uL 3:3,A 14 FC2G 455S014 L18989-4,, 1,WG42273,15/4Q00uL 3:3,B 15 FC2G 455S015 L18989-5,, 1,WG42273,15/4000uL 3:3,C 16 FC2G 455S016 L18989-6,, 1,WG42273,15/4000uL 3:3,D 17 FC2G 455S017 L18989-7,, 1,WG42273,15/4000uL 3:3,E 18 FC2G 455S018 L18989-8,, 1,WG42273,15/4000uL 3:3,F 19 FC2G 455S019 L18989-9,, 1,WG42273,15/4000uL 3:4,A 20 FC2G 455S020 L18989-10,, 1,WG42273,15/4000uL 3:4,B 21 FC2G 455S021 L18989-11,, 1,WG42273,15/4000uL 3:4,C 22 FC2G 455S022 WG42273-103,,DUP 1,WG42273,15/4000uL 3:4,D 23 FC2G 455S023 L18989-12,, 1,WG42273,15/4000uL 3:4,E 24 FC2G 455S024 L18989-13,, 1.WG42273,15/4000uL 3:4,F 25 FC2G 455S025 L18989-14,, 1,WG42273,15/4000uL 3:5,A 26 FC2G 455S026 L18992-1,, 1,WG42273,15/4000uL 3:5,B 27 FC2G 455S027 L18992-2,, 1,WG42273,15/4000uL 3:5,C 28 FC2G 455S028 FC-L1N-42221W500-D,, 1,,15.uL 1:1, D 29 FC2G 455S029 FC-LIN-42221W500-D,, 1,,15uL 1:1,D 30 FC2G 455S030 MeOH,, 1,,15uL ' 1:8,A 31 FC2G 455S031 MeOH,, 1,,15uL 1:8,A r Jr r -- _ . --" - L/ . " . ." - T I - _ - -- - . - , . . . - 7 15.0 15.0 15.0 -n 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 \ 15.0 15.0 15.0 15.0 15.0 15.0 : 15.0 15,0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 ^ 15.0 Page 214 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G_455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: FC-LIN-42221W 500-D,, Name: FC2G_455S004 Description: 1,,15uL Date: 18-Dec-2012 Time: 18:49:50 PFBA FC2G_455S004 100- 5 .1 8 4 .5 5 e 4 148574 5 .0 0 6.00 7.00 PFNA FC2G 455S004 PFHxS-1 FC 2G _455S 004 100- 7 .2 5 /T 2 .5 7 e 4 J .98811 %- PFPeA FC2G 455S004 100- 5 .9 6 6 .33e4 " 320827 1 ; 0 - -1 m in 5 .0 0 6 .0 0 7.00 PFDA FC2G_455S004 100- 7 .9 3 1 .3 8 e 5 I 492967 8.00 10.00 12.00 PFHxS-2 FC2G 455S004 100- 7 .3 0 1.3 4 e 4 i 50461 %- PFHxA FC2G 455S004 100- 6 .3 6 1.15 e5 577808 : %: j . 0 - - - - - .................. i 5 .0 0 6 .0 0 7.00 PFHpA FC2G_455S004 100- 6 .6 9 1.19 e 5 487609 1 %- o5 .0 0 6 .00 7.00 PFUnA FC2G 455S004 100- 8 .5 0 F 1.44e5. 1 612888 1 1 0 - m in 8.00 10.00 12.00 PFDoA FC2G 455S004 . 100- 9 .0 6 f " 1 .54e5 j| 6 6 5 6 3 6 %~ H 1 0- 8.00 ................ pr min 10.00 12.00 PFHxS-3 FC2G 100- 455S004 7 .2 5 A 8.26e3 / \ 30973 PFOS-1 FC2G_455S004 100- 8 .2 3 r 2.37e4 I 65039 %- PFOA-1 FC2G 455S004 100^ 7 .1 1 A 1.05e5 i .430210 %: I \ - !\ 0J . U - ____ _______ 7 .0 0 8 .0 0 PFBS-1 FC2G 100^ %- 6 .4 5 2.9 6 e4 | 142625 o: 5 .00 6 .00 7.00 P F O S-2 FC2G_455S004 mn 8-23 100^ f 1.51e4 52888 %- " 7 .0 0 8.00 8.00 7 .0 0 8.00 8.00 9 .9 0 10.00 12.00 8.00 10.00 12.00 PFOA-2 FC2G 455S004 100" 7.11 J T 2-77e4 Q 113863 %- ' i 0" . .. 7 .0 0 8.00 PFBS-2 FC2G_455S004 100 6 .4 7 1 .5 4 e 4 l 75453 I 5 .0 0 J\ . 6 .0 0 7.00 PFOSA FC 2G _455S 004 100- 9 .9 0 2 .2 8 e 4 l 80135 1 % 8.00 10,00 12.00 PV by LHE 08-Jan-13, SV byAP 10-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 215 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 D a ta se t: G:\Masslynx\lnst_G\PFC.PRO\FC\FC2G__455-B.qld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: FC-LIN-42221W 500-D,, Name: FC2G_455S004 Description: 1,,15uL Date: 18-Dec-2012 Time: 18:49:50 13C2-PFOA FC2G_455S004 100- 7.11 I T 1.87e5 n 754666 %- 13C4-PFOA FC2G 455S004 100n 7 .0 6 A 6 .4 1e4 / 251133 %~ ' = o7 .0 0 13C4-PFBA FC2G 455S004 . . m in 8.00 0 - ........... . . . m in 7 .0 0 8.00 13C2-PFHxA FC2G_455S004 100- 6.3 4 6 .6 6 e 4 ' 325053 %- 5.00 6.00 7.00 13C4-PFOS-1 FC2G 455S004 8 .2 3 100-, 1.03e4 | 39661 %I 0" m in 8.00 10.00 12.00 13C5-PFNA FC 2G _455S 004 100- 7 .4 6 6 .5 6 e 4 f, 277931 j \ %- 7 .0 0 8.00 13C4-PFOS-2 FC2G 455S004 100-1 8 .2 3 f 7 .82e3 if 3 0 9 7 8 %-- j: J ifL i 0 m in 8.00 10.00 12.00 1802-PFHxS-1 FC2G 455S004 10 0 ~1 7 .3 0 j T 9.76e3 J J .35470 %-- '' ,, 0-J ......... 11 7 .0 0 8 .0 0 m in 13C2-PFDA FC 2G ,,455S 004 7 .9 3 100J1n i~ 7 .3 9 e 4 - ; 268434 %H 1 i0- 8.00 -- min 10.00 12.00 13C2-PFDoA FC2G__455S004 100- 9.06 f 9.73e4 429607 %- 0- -- 8.00 10.00 12.00 1802-PFHxS-2 FC2G 455S004 100- 7 .3 0 % 6.82e3 1 28975 %-- i 7 .0 0 8 .0 0 1 3 C 2-P FO U EA FC2G_455S004 100- 7 .3 9 2 :2 6 e 4 'l 90898 i %- O-F 7 .0 0 min 8.00 PV by LHE 08-Jan-13, SV by AP 10-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 216 of 307 www.axysanalytical.com Quantify Sample Report M assLyn x 4.1 SCN 627 Dataset: G :\M a s s ly n x \ln s t_ G \P F C .P R O \F C \F C 2 G _ 4 5 5 -B .q ld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: FC-LIN-42221W 500-D,, Name: FC2G_455S004 Description: 1,,15uL Date: 18-Dec-2012 Time: 18:49:50 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFO A-2 7 PFNA 8 PFDA 9 PFUnA 10 P F D oA 11 P F B S -1 . 12 PFBS-2 13 PFHxS-1 14 PFH xS-2 15 P FH xS-3 16 PFOS-1 17 PFOS-2 18 P FO SA 19 13C2-PFOA 20 13C 4-P FO A 21 1 3C 4-P F O S -1 2 2 13C4-PFOS-2 23 1802-P F H xS -l 24 1802-PFFIxS-2 25 13C 4-P FB A 26 13C 2-P FH xA 27 13C 5-P FN A 28 13C 2-P FD A 29 13C 2-P FD oA 30 13C 2-P FO U E A . IS# Trace RT 25 2 1 2 .9 9 > 1 6 8 .9 8 5.18 26 2 6 2 .9 8 > 2 18.... 5.96 26 3 1 2 .9 7 > 2 68 .98 6.3 6 26 3 6 2 .9 7 > 3 18 .98 6.69 19 4 1 2 .9 7 > 3 6 8 .9 8 7.11 19 4 1 2 .9 7> 1 68 .80 7,11 27 4 62 .96 > 4 1 8 .... 7.46 28 5 1 2 .9 6 > 4 68 .... 7.93 28 5 6 2 .9 6 > 518 .... 8.50 29 6 1 2 .9 5 > 568 .... 9.06 23 2 9 8 .9 4 > 79.96 6.45 24 2 9 8 .9 4 > 98.9 5 6.47 23 3 9 8 .8 4 > 79.96 7.2 5 24 3 9 8 .8 4 > 98.9 6 7.30 23 3 9 8 .8 4> 118.70 7.25 21 4 9 8 ,9 3> 7 9,9 6 8 .2 3 22 4 9 8 .9 3> 98.9 6 8.23 21 4 9 8 > 7 8 9 ,9 0 20 4 1 4 .9 7 > 3 6 9 .... 7.11 4 1 6 .9 7 > 371.9 7.06 30 503 > 79.96 8 ,2 3 30 503 > 98.96 30 4 0 2 .9 > 8 4 8 .2 3 7 .3 0 30 402.9>103 7 .3 0 30 2 1 6 .9 9 > 1 71,98 5.1 8 30 3 1 4 ,9 7 > 2 6 9 .9 8 6.34 30 4 6 7 .9 6 > 4 2 2 .9 7 7 .4 6 30 5 1 4 .9 6 > 4 6 9 .9 6 7.9 3 30 6 1 4 .9 5 > 569 .... 9.06 4 5 8 :9 8 > 3 9 3 .9 8 7.39 Area 45497 63301 115119 119037 105234 27721 113135 137589 144066 . 153785 29617 15379 25652 13409 8264 23725 15076 22802 187308 64121 10329 7824 9756 6818 29865 66645 65558 73922 97271 22615 IS Area ng/L 29865 1 7.4 G 6 66645 1 6 .5 8 4 66645 1 9 .0 0 7 66645 1 8 .0 5 7 187308 18.757 187308 18.920 65558 1 9 .8 0 5 73922 2 0 .9 2 7 73922 1 7 .6 4 2 97271 2 0 .5 1 3 9756 4 0 .7 1 3 6818 3 8 .7 8 1 9756 3 9 .4 5 3 6818 9756 3 6 .5 6 9 38.010 10329 3 9 .0 8 8 7824 10329 3 9 .6 0 6 18.056 64121 3 4 ,5 4 9 22615 1 5 .4 2 5 1 8 .4 0 2 22615 1 8 .8 1 8 22615 2 0 .0 1 6 22615 1 9 .6 3 5 22615 . 12.448 22615 1 2 .9 0 3 22615 1 2 .7 8 9 22615 22615 1 1 .9 1 2 10.669 1 1 .5 6 2 LOD 0 .0 4 63 0 .1 2 7 5 0 .1 4 5 2 0 .0 3 1 0 0 .0 6 0 3 0 .0 7 0 9 0 .2 7 0 4 0 .5 1 6 9 0 .1 1 2 5 0 .0 0 1 5 0 .2 4 2 2 0.1116 0 .1829 0 .1 2 4 2 0 .0 4 6 3 0 .2727 0 .0126 0 .0 3 9 4 0 .0 8 3 2 0 .0 2 8 1 %Rec 8 7 .0 8 2 .9 9 5 .0 9 0 ,3 9 3 .8 9 4 .6 9 9 .0 . 1 0 4 .6 8 8 .2 1 0 2 .6 1 0 1 .8 9 7 .0 9 8 .6 9 1 .4 95.0 97.7 9 9 .0 9 0 .3 9 6 .0 1 2 8 .5 1 0 2 .2 1 0 4 .5 1 1 1 .2 109.1 1 0 3 .7 107.5 1 0 6 .6 9 9 .3 8 8 .9 1 1 5 .6 S/N 388 2367 3401 1991 0 0 1257 954 1718 1500 2627 2465 0 331 858 279 260 710 0 415 288 448 1174 0 106 3724 0 820 423 1234 Noise 383 136 170 245 0 0 390 517 357 444 54 31 0 153 36 233 203 113 0 606 138 69 30 877 87 0 327 1016 74 Height 148574 320827 577808 487609 430210 113863 490086 492967 612888 665636 142625 75453 98811 50461 30973 65039 52888 80135 754666 251133 39661 30978 35470 28975 92484 325053 277931 268434 429607 90898 IS Height 92484 325053 325053 325053 754666 754666 277931 268434 268434 429607 35470 28975 35470 28975 35470 39661 30978 39661 251133 90898 90898 90898 90898 90898 90898 90898 90898 90898 Sample size 1 .0 0 0 0 1 .0 0 0 0 1 .0 0 0 0 1 .0000 1 .0000 1 .0000 1 .0 0 0 0 1 .0000 1 .0000 1 .0000 1 .0 0 0 0 1 .0000 1 .0 0 0 0 1 .0 0 0 0 1.0000 1 .0000 1 .0000 1 .0000 1 .0000 1 .0000 1 .0000 1 .0 0 0 0 1 .0000 1 .0000 1 .0000 1 .0000 1 .0000 1 .0000 1 .0 0 0 0 1.0 0 00 Factor2 Detection... 1.0 bb 1 .0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 M M 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 M M 1.0 M M 1.0 MM 1.0 bb 1.0 M M 1.0 bb 1.0 bb 1.0 M M 1.0 bb 1.0 M M 1.0 bb 1.0 bb 1.0 bb 1 .0 bb 1.0 bb 1.0 bb 1.0 bb PV by LHE 08-Jan-13, SV by AP 10-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 217 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 Dataset: G :\M a s s iy n x \ln s t_ G \P F C .P R O \F C \F C 2 G _ 4 5 5 -B .q ld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: FC-L1N-42221W500-D,, Name: FC2G_455S028 Description: 1,,15uL Date: 19-Dec-2012 Time: 01:53:10 PFBA FC2G__455S028 . . . 5.16 100-1 l~ 4 .1 0 e4 ;i 141011 %' PFPeA FC 2G _455S 028 100- 5 .9 8 5 .6 1e4 278739 ; 0- 5 .00 6 .0 0 7.00 PFNA FC2G 455S028 5.00 6 .00 7.00 PFDA FC2G_455S028 mn 7-93 100^ F 1.17e5 I 421843 %- PFHxA FC 2G ,,455S 028 100- 6 .3 7 _ 9 .74e4 484497 5 .00 6 .0 0 7.00 PFUnA FC2G__455S028 100- 8 .4 7 F 1.27e5 I 556113 %- PFHxS-1 FC2G 455S028 8.00 10.00 12.00 PFHxS-2 FC2G_455S028 100- 7 ,3 0 A 1.14e4 M 45905 / 0 - ---------- i ; 7 .0 0 A ......... ..... .. T 8.00 m in 8.00 10.00 12.00 o o _l_J PFHxS-3 FC2G__455S028 7 ,3 0 A 6.84e3 * i \ 26461 %- ; nj 7 .0 0 8 .0 0 PFHpA FC2G_455S028 100- 6 .7 4 1 .0 8 e 5 454795 j %- 5 .00 6 .0 0 7.00 PFDoA FC2G_455S028 100- 9 .0 3 F 1.34e5 541445 %- 0 --------min 8.00 10.00 12.00 PFOS-1 FC 2G _455S 028 100- 8 .2 3 r 2.09e4 60602 8.00 9 .9 0 10.00 12.00 PFOA-1 F C2G__455S028 100- 7.11 K 9.00e4 ,36 7 40 6 7 .0 0 8.00 PFBS-1 FC2G_455S028 100- 6 .4 7 2 .6 3 e 4 126264 | h %- 5 .00 6 .00 7.00 PFOS-2 F C 2G ,,455S 028 100- _ 8.23 1 .42e4 47419 /l 8.00 10.00 12.00 PFOA-2 FC2G_455S028 100- 7.11 A 2 .5 4 e4 t 103379 %- 0- 7 .0 0 8.00 PFBS-2 FC2G_455S028 100- 647 1.35e4 | 6 7 3 0 5 ft % 5 .0 0 6.0 0 7.00 PFOSA F C 2G ,,455S 028 -m n 100^ 9 .9 0 2 .0 1 e 4 l 6 7 4 2 0 :: 8.00 10.00 12.00 PV by LHE 08-J.an-13, SV by AP 10-Jan-13. NOTE: OPR Concn..& LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 218 of 307 www.axysanalytical.com Quantify Sam ple Report M a ssLyn x 4.1 SCN 627 Dataset: G :\M a sslyn x\ln st_ G \P F C .P R O \F C \F C 2 G _4 5 5 -B .q ld LastA itered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: FC-LIN-42221W500-D,, Name: FC2G_455S028 Description: 1,,15uL Date: 19-Dec-2012 Time: 01:53:10 1 3 C 2 -P FO A FC2G 455S028 100-1 `A 1.62e5 . .1682835 %- /' 0 - --------- -- ----- -TT mmin 7.00 8.00 13C4-PFOA FC2G_455S028 too- / 5.19e4 r \ 213907 %- r\ u0--' - - ........... ................. ! 7.00 8.00 13C4-PFBA FC2G_455S028 100 __ 5,13 a 2.60e4 W 90343 %- 4.06 i : Jj 5.00 6 .00 7.00 13C2~PFHxA FC2G 455S028 100- 5 .6 4 e 4 l 2 8 1 0 0 6 (1 %-- i\ o - ---------------------- A 5 .0 0 6 .0 0 7.00 13C4-PFOS-1 FC2G 455S028 8.23 100-n T 9.30e3 J 34762 %~ I ft 0 - rrrrr min 8.00 10.00 12.00 13C4-PFOS-2 FC2G 455S028 100- 8.23 I 7.32e3 - 28023 % - i'i il 0 ----- 8.00 10.00 12.00 13C5-PFNA FC2G_455S028 100- 7.46 5.88e4 A 245524 ` \ %- 13C2-PFDA FC2G_455S028 100- _ 7.93 i 5.96e4 i 209570 %- 7.00 8.00 8.00 10.00 12.00 1802-PFHxS-1 FC2G 455S028 100- 7.30 /C~9.17e3 14 37990 1802-PFHxS-2 FC2G 455S028 100-, 7.30 i\ 5.63e3 ; \ 20561 %- 0- : ------ '.. -- ---- ----r miin 7 .0 0 8 .0 0 u0 ------ --/-------- x- ,---- --' 7 .0 0 8 .0 0 13C2-PFDoA FC2G__455S028 100- _ 9.03 8.51e4 360513 13C 2-P FO U EA FC2G_455S028 100- 7:39 _ 2.05e4 83993 ' \ % -- 8.00 10.00 12.00 7.00 8.00 PV by LHE 08-Jan-13, SV byAP 10-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 219 of 307 www.axysanalytical.com Quantify Sam ple Report M a ssLyn x 4.1 SCN 627 Dataset: G :\M a sslyn x\ln st_ G \P F C .P R O \F C \F C 2 G _ 4 5 5 -B .q ld Last Altered: Tuesday, January 08, 2013 12:06:04 Pacific Standard Time Printed: Thursday, January 10, 2013 12:48:05 Pacific Standard Time ID: FC-LIN-42221W 500-D,, Name: FC2G_455S028 Description: 1,,15uL Date: 19-Dec-2012 Time: 01:53:10 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-l 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA , 27 13C5-PFNA 28 13C2-PFDA 29 1302-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.16 26 262.98 > 218.... 5.98 26 312.97> 268.98 6.37 26 362.97> 318.98 6.74 19 412.97> 368.98 7.11 19 412.97> 168,80 7.11 27 462.96 >418.... 7.49 28 512.96 >468.... 7.93 28 562.96 >518.... 8.47 29 612.95 >568.... 9.03 23 298,94> 79.96 6.47 24 298.94 > 98,95 6.47 23 398.84 > 79.96 7.30 24 398.84> 98.96 7.30 23 398.84> 118.70 7.30 21 498.93> 79.96 8.23 22 498.93> 98.96 8.23 21 498 >78 . 9.90 20 414.97 > 369.... 7.11 416.97 >371,9 7.11 30 503 > 79.96 8.23 30 503 > 98.96 8.23 30 402.9>84 7.30 30 402.9>103 7.30 30 216.99>171.98 5.13 30 314.97>269.98 6.36 30 467.96>422.97 7.46 30 514.96 >469.96 7.93 30 614.95 >569.... 9.03 458.98 >393.98 7.39 Area 41016 56070 97374 108302 89975 25352 99068 116730 127263 133628 26264 13548 21609 11398 6839 20943 14192 20137 162308 51893 9298 7322 9166 5634 26015 56401 58805 59559 85139 20521 IS Area 26015 56401 56401 56401 162308 162308 58805 59559 59559 85139 9166 5634 9166 5634 9166 9298 7322 9298 51893 20521 20521 20521 20521 20521 20521 20521 20521 20521 ng/L 18.018 17.353 18.998 19.405 18.506 19.967 19.334 22.058 19.347 20.362 38.412 41.330 35.341 37.610 33.443 38.330 39.840 17.721 36.992 12.484 18.254 19.409 20.723 17.880 11.950 12.033 12.642 10.577 10.291 10.492 LOD 0.1348 0.1330 0.1480 0.0301 0.1145 0.2740 0.3416 0.1607 0,0350 0.2375 0.2087 0.1376 0.3291 0.0381 0.2614 0.0182 0.0454 0.0723 0.0243 %Rec 90.1 86.8 95.0 97.0 92.5 99.8 96.7 110.3 96.7 101.8 96.0 103.3 88.4 94.0 83.6 95.8 99.6 88.6 102.8 104.0 101.4 107.8 115.1 99.3 99.6 100.3 105.4 88.1 85.8 104.9 S/N 569 1780 3135 4653 2065 494 3124 1526 901 1370 1818 2906 483 1061 205 399 393 729 0 179 363 165 0 1255 116 2408 0 601 442 1294 Noise 248 157 155 98 178 209 129 276 617 395 69 23 177 43 129 152 121 92 0 1192 96 169 0 16 776 117 0 349 816 65 Height 141011 278739 484497 454795 367406 103379 404222 421843 556113 541445 126264 67305 85496 45905 26461 60602 47419 67420 682835 213907 34762 28023 37990 20561 90343 281006 245524 209570 360513 83993 IS Height 90343 281006 281006 281006 682835 682835 245524 209570 209570 360513 37990 20561 37990 20561 37990 34762 28023 34762 213907 83993 83993 83993 83993 83993 83993 83993 83993 83993 Sample size 1.0000 1.0000 1.0000 1,0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1,0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1,0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb PV by LHE 08-Jan-13, SV by AP 10-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 220 of 307 www.axysanalytical.com ______________________4 B | M ___________________ ;_______________________ Sample List Report M assLynx 4.1 SCN 627 Sample List: G:\Masslynx\lnst_G\_Master.PRO\SampleDB\FC3G_015.SPL Last Modified: Monday, January 14, 2013 15:51:45 Pacific Standard Time Printed: Monday, January 14, 2013 15:58:34 Pacific Standard Time Page 1 of 1 Page Position (1,1) File Name Sample ID File Text Bottle s ignln SignOut Inj VoL 1 FC3G 015S001 MeOH,, 2 FC3G 015S002 MeOH,, 1,,15uL 1,,15uL 1:8,A 1:8,A L\ -- u AJ A 15.0 15.0 3 FC3G 015S003 FC-Ll N-42420W500-A,, 1,,15uL 2:1,A - H 15.0 4 FC3G 015S004 FC-LIN-42420W500-A,, 1,,15uL 2:1,A - - ' -- 15.0H 5 FC3G 015S005 FC-Ll N-42420W500-B,, 1,,15uL 2:1,B - " -J 15.0 | 6 FC3G 015S006 FC-LI N-42420W500-C,, 1,,15uL 2:1,C - -4 15.0 7 FC3G 015S007 FC-L1N-42420W500-D,, 1,,15uL 2:1 ,D " -4 15.0 ) 8 FC3G 015S008 FC-LIN-42420W500-E,, 1,,15uL 9 FC3G 015S009 FC-LI N-42420W500-F,, 1,,15uL 2:1,E - " 2:1,F - "T -X 15.0 15.0 10 FC3G 015S010 FC-LI N-42420W500-G,, 1,,15uL 2:2,A - "f 15.0 j 11 FC3G 015S011 FC-UN-42420W500-H,, 1,,15uL 12 FC3G 015S012 MeOH,, 1,,15uL 2:2,B 1:8,A " 1" ~i 15.0 " 15.0 13 FC3G 015S013 MeOH,, 1,,15uL 1:8,A " "4 15.0 14 FC3G 015S014 FC-42420W500-SAR2,, 1,,15uL 2:2, C - -j * 15.0 15 FC3G 015S015 FC-42420W500-SAR,, 1,,15uL 2:2,D - - 15.0 16 FC3G 015S016 FC-LIN-42420W500-D,, 1,,15uL 2:1,D - -- 15.0 17 FC3G 015S017 FC-LIN-42420W500-D,, 1,,15uL 2:1, D* - -- 15.0 18 FC3G 015S018 WG42428-103,,SAR 1,WG42428,15/4000uL 2:2,E - - - 15.0 19 FC3G 015S019 WG42428-102,,SPM 1,WG42428,15/4000uL 2:2, F- ** " --* 15.0 20 FC3G 015S020 MeOH,, 1,,15uL 1:8,A -- 15.0 21 FC3G 015S021 MeOH,, 22 FC3G 015S022 WG42428-101 ,,BLK 1,,15uL 1:8,A " 1,WG42428,15/4000uL 2:3,A ' " " -- -- 15.0 15.0 23 FC3G 015S023 L18989-1 ,R, 24 FC3G 015S024 L18989-2,R, 1,WG42428,15/4000uL 2:3,B- 1,WG42428,15/4000uL 2:3,C " - -- -- 15.0 15.0 25 FC3G 015S025 L18989-3,R, 1,WG42428,15/4000uL 2:3,D - - -- 15.0 26 FC3G 015S026 L18989-4,R, 1,WG42428,15/4000uL 2:3,E - -- 15.0 27 FC3G 015S027 L18989-5,R, 1,WG42428,15/4000uL 2:3,F - - -- 15.0 28 FC3G 015S028 L18989-6,R, 1,WG42428,15/4000uL 2:4,A- - -- 15.0 29 FC3G 015S029 L18989-7, R, 1,WG42428,15/4000uL 2:4,B -- -- 15.0 30 FC3G 015S030 L18989-8,R, 1,WG42428,15/4000uL 2:4,C -" -- 15.0 31 FC3G 015S031 L18989-9,R, 32 FC3G 015S032 L18989-10.R, 1,WG42428,15/4000uL 2:4,D 1,WG42428,15/4000uL 2:4, E -- -- 15.0 15,0 33 FC3G 015S033 L18989-12,R, 1,WG42428,15/4000uL 2:4, F* - -- 15.0 34 FC3G 015S034 L18989-13.R, 1,WG42428,15/4000uL 2:5,A - -- 15.0 35 FC3G 015S035 36 FC3G 015S036 37 FC3G 015S037 L18989-14.R, FC-LI N-42420W500-D,, FC-LI N-42420W500-D,, 1,WG42428,15/4000uL 1,,15uL 1,,15uL 2:5,B 2:1,D 2:1,D "r__. 1 -- -- -- 15.0 15.0 15.0 38 FC3G 015S038 MeOH,, 39 FC3G 015S039 MeOH,, 1,,15uL 1,,15uL 1:8,A 1:8,A ?t -j 15.0 15.0 Page 221 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Method: G:\Masslynx\Master Methods\PFC\FC_060_10_01.mdb 03 Dec 2010 17:21:03 Calibration: 15 Jan 2013 10:48:13 Compound name: PFBA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 5.21 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 5.19 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 5.19 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 5.23 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 5.24 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 5.23 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 5.23 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 5.24 Area 2152 5517 17665 63528 301419 500633 1092578 2094684 IS Area 37393 38330 38179 37645 35113 30026 24526 20307 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.461 1.442 5.062 18.984 97.717 190.827 518.258 1243.976 %Rec 92.2 115.3 101.2 94.9 97.7 95.4 103.7 99.5 S/N 10 48 377 575 6913 5 7187 5 Compound name: PFBA Coefficient of Determination: RA2 = 0.999381 Calibration curve: -4.99187e-005 * xA2 + 1.05696 * x + 0.203358 Response type: Internal Std ( Ref 25 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 222 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFPeA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 5.95 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 5.95 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 5.97 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 5.95 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 5.97 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 5.97 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 5.95 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 5.97 Area 2502 6331 20792 80737 379493 670319 1417364 2925513 IS Area 72936 77481 77682 73854 68145 58258 47890 40957 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.547 1.361 4.557 18.749 95.772 198.161 511.694 1245.910 %Rec 109.3 108.9 91.1 93.7 95.8 99.1 102.3 99.7 Compound name: PFPeA Coefficient of Determination: RA2 = 0.999699 Calibration curve: -8.27166e-006 * xA2 + 0.698255 * x + 0.0299181 Response type: Internal Std ( Ref 26 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 50 141 429 1512 8374 16850 22502 50663 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 223 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFHxA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 6.36 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 6.34 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 6.36 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 6.34 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 6.36 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 6.36 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 6.33 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 6.34 Area 4834 11980 36978 140318 632521 1071639 2165990 4181689 IS Area 72936 77481 77682 73854 68145 58258 47890 40957 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.469 1.402 4.796 19.866 98.807 198.222 505.086 1248.015 %Rec 93.8 112.1 95.9 99.3 98.8 99.1 101.0 99.8 Compound name: PFHxA Coefficient of Determination: RA2 = 0.999940 Calibration curve: -0.000124546 * xA2 + 1.13695 * x + 0.261958 Response type: Internal Std ( Ref 26 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 142 213 688 6800 15254 26475 49340 50289 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 224 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFHpA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 6.73 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 6.73 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 6.73 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 6.73 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 6.73 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 6.73 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 6.73 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 6.73 Area 3752 10472 39072 144182 638236 1139370 2262452 4711523 IS Area 72936 77481 77682 73854 68145 58258 47890 40957 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.459 1.325 5.132 20.144 97.204 203.894 498.311 1250.282 Compound name: PFHpA Coefficient of Determination: RA2 = 0.999912 Calibration curve: -4.45074e-005 * xA2 + 1.15968 * x + 0.0851693 Response type: Internal Std ( Ref 26 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None %Rec 91.8 106.0 102.6 100.7 97.2 101.9 99.7 100.0 S/N 105 189 1062 3803 12076 27466 53152 91806 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 225 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFOA-1 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.14 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.09 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.14 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.14 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.14 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.14 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.09 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.09 Area 4717 12440 40626 149341 692014 1197420 2464122 4933775 IS Area 251816 264525 265618 265755 240757 211297 180448 143912 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.406 1.416 5.199 19.811 102.573 202.861 491.806 1252.684 %Rec 81.1 113.3 104.0 99.1 102.6 101.4 98.4 100.2 S/N 39 74 0 0 0 0 0 0 Compound name: PFOA-1 Coefficient of Determination: RA2 = 0.999854 Calibration curve: -1.8414e-005 * xA2 + 1.0081 * x + 0.265557 Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 226 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFOA-2 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.14 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.09 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.09 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.14 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.14 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.14 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.09 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.09 Area 1255 2315 9021 34651 171424 286450 592967 1165768 IS Area 251816 264525 265618 265755 240757 211297 180448 143912 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.570 1.124 4.835 19.037 105.039 200.887 492.779 1252.495 %Rec 113.9 90.0 96.7 95.2 105.0 100.4 98.6 100.2 S/N 77 83 169 1499 0 0 0 0 Compound name: PFOA-2 Coefficient of Determination: RA2 = 0.999777 Calibration curve: -9.45627e-006 * xA2 + 0.244644 * x + 0.0399976 Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 227 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFNA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.55 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.55 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.55 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.55 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.55 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.55 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.49 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.49 Area 3856 10365 36435 144493 643515 1146232 2391985 5089518 IS Area 76162 83747 83846 83869 79478 71910 55823 45876 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.436 1.338 5.169 21.027 98.921 193.521 508.363 1247.932 Compound name: PFNA Coefficient of Determination: RA2 = 0.999798 Calibration curve: 7.51007e-005 * xA2 + 0.972924 * x + 0.183187 Response type: Internal Std ( Ref 27 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None %Rec 87.2 107.0 103.4 105.1 98.9 96.8 101.7 99.8 S/N 43 144 578 13918 0 22371 36654 0 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 228 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFDA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 8.05 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 8.05 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 8.05 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 8.05 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 8.05 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 8.05 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 8.05 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 8.05 Area 5923 14790 48705 185619 879693 1420377 3073619 6104199 IS Area 75970 97950 95963 98960 93731 81150 83939 84417 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 Compound name: PFDA Coefficient of Determination: RA2 = 0.998644 Calibration curve: -0.000311529 * xA2 + 1.07752 * x + 0.469487 Response type: Internal Std ( Ref 28 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None ng/L 0.433 1.246 5.225 20.576 107.421 206.864 471.682 1274.461 %Rec 86.5 99.7 104.5 102.9 107.4 103.4 94.3 102.0 S/N 89 377 356 1253 4480 4875 8972 3540 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 229 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFUnA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 8.61 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 8.61 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 8.61 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 8.61 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 8.61 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 8.64 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 8.61 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 8.58 Area 5269 14561 51692 199149 946327 1622024 3473490 7620483 IS Area 75970 97950 95963 98960 93731 81150 83939 84417 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.439 1.247 5.224 20.315 104.927 213.112 468.725 1266.699 %Rec 87.9 99.8 104.5 101.6 104.9 106.6 93.7 101.3 S/N 74 240 724 1495 4936 8175 9193 7802 Compound name: PFUnA Coefficient of Determination: RA2 = 0.998354 Calibration curve: -0.000255394 * xA2 + 1.17845 * x + 0.314693 Response type: Internal Std ( Ref 28 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 230 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFDoA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 9.24 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 9.24 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 9.24 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 9.21 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 9.21 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 9.24 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 9.21 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 9.18 Area 6475 15981 64757 244382 1081028 2106076 4341857 9446096 IS Area 136364 132608 158117 159917 146991 148678 149909 173162 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.366 1.374 5.370 20.934 104.702 209.250 470.764 1285.038 %Rec 73.2 109.9 107.4 104.7 104.7 104.6 94.2 102.8 Compound name: PFDoA Coefficient of Determination: RA2 = 0.998632 Calibration curve: -0.000280667 * xA2 + 0.86988 * x + 0.251461 Response type: Internal Std ( Ref 29 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 111 224 717 2177 3889 11005 9659 11577 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 231 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFBS-1 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 6.51 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 6.47 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 6.49 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 6.49 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 6.49 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 6.49 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 6.47 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 6.49 Area 1250 2925 10293 41241 176606 287793 574749 1021844 IS Area 11409 11723 12752 11961 10750 9307 7532 5162 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 0.825 2.636 9.852 44.008 211.633 398.208 978.718 2507.622 Compound name: PFBS-1 Coefficient of Determination: RA2 = 0.999617 Calibration curve: 1.18725e-005 * xA2 + 1.39094 * x + 0.824691 Response type: Internal Std ( Ref 23 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None %Rec 82.5 105.4 98.5 110.0 105.8 99.6 97.9 100.3 S/N 141 544 2250 2292 36944 15577 76153 48512 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 232 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFBS-2 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 6.51 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 6.49 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 6.49 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 6.47 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 6.47 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 6.51 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 6.47 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 6.47 Area 706 1423 5529 20643 93835 150714 292314 526024 IS Area 8167 9543 9276 8817 8538 6193 5239 4007 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 1.157 2.223 9.821 39.540 187.968 420.913 990.315 2501.618 %Rec 115.7 88.9 98.2 98.8 94.0 105.2 99.0 100.1 Compound name: PFBS-2 Coefficient of Determination: RA2 = 0.999469 Calibration curve: -4.58769e-005 * xA2 + 1.05926 * x + 0.33002 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 67 198 717 4112 8405 18009 31388 51919 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 233 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFHxS-1 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.33 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.33 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.33 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.33 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.33 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.33 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.33 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.33 Area 1177 2758 8511 35764 152325 267940 516587 912450 IS Area 11409 11723 12752 11961 10750 9307 7532 5162 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 0.904 2.798 8.991 42.269 202.336 411.296 978.341 2506.565 %Rec 90.4 111.9 89.9 105.7 101.2 102.8 97.8 100.3 Compound name: PFHxS-1 Coefficient of Determination: RA2 = 0.999732 Calibration curve: 5.25128e-006 * xA2 + 1.256 * x + 0.720654 Response type: Internal Std ( Ref 23 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 0 0 139 281 0 10903 0 0 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... bb MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 234 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFHxS-2 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.33 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.33 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.33 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.33 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.33 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.33 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.33 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.33 Area 630 1261 4912 17711 86045 141803 284063 503432 IS Area 8167 9543 9276 8817 8538 6193 5239 4007 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 1.288 2.274 9.407 35.989 181.967 417.389 1012.035 2493.009 %Rec 128.8 90.9 94.1 90.0 91.0 104.3 101.2 99.7 S/N 89 0 0 3728 0 4240 7540 0 Compound name: PFHxS-2 Coefficient of Determination: RA2 = 0.999170 Calibration curve: -3.85889e-005 * xA2 + 1.00334 * x + 0.0966945 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 235 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFHxS-3 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.33 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.33 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.33 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.33 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.33 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.33 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.33 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.33 Area 282 625 3012 10989 51386 82734 155131 296727 IS Area 11409 11723 12752 11961 10750 9307 7532 5162 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 0.863 2.208 10.815 42.899 223.279 413.136 942.917 2517.457 %Rec 86.3 88.3 108.2 107.2 111.6 103.3 94.3 100.7 S/N 25 51 102 0 0 4172 0 0 Compound name: PFHxS-3 Coefficient of Determination: RA2 = 0.998390 Calibration curve: 1.13903e-005 * xA2 + 0.382314 * x + 0.115235 Response type: Internal Std ( Ref 23 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 236 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFOS-1 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 8.41 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 8.41 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 8.41 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 8.41 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 8.35 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 8.41 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 8.35 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 8.35 Area 1211 2764 9055 33164 162731 271377 560878 1099194 IS Area 13097 14831 14361 14492 14226 11098 8899 6445 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 1.006 2.605 10.163 38.306 191.828 405.150 1006.719 2497.579 %Rec 100.6 104.2 101.6 95.8 95.9 101.3 100.7 99.9 S/N 34 44 126 522 1675 1808 3373 3626 Compound name: PFOS-1 Coefficient of Determination: RA2 = 0.999890 Calibration curve: 6.88659e-005 * xA2 + 1.057 * x + 0.600346 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 237 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFOS-2 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 8.41 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 8.41 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 8.41 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 8.35 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 8.35 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 8.41 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 8.35 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 8.35 Area 650 1840 6360 22836 118969 190358 387780 759759 IS Area 10291 11508 11454 11374 11600 8840 6993 5284 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/L 0.946 2.777 10.251 37.683 192.444 401.314 1012.010 2496.000 %Rec 94.6 111.1 102.5 94.2 96.2 100.3 101.2 99.8 S/N 37 78 131 314 1585 1716 2172 4089 Compound name: PFOS-2 Coefficient of Determination: RA2 = 0.999861 Calibration curve: 3.41978e-005 * xA2 + 0.951493 * x + 0.236478 Response type: Internal Std ( Ref 22 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 238 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: PFOSA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 9.99 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 9.99 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 9.99 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 9.99 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 9.99 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 9.99 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 9.99 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 9.99 Area 538 1521 7114 28634 115291 222311 531646 1222304 IS Area 13097 14831 14361 14492 14226 11098 8899 6445 Std. Conc 0.500 1.250 5.000 20.000 100.000 200.000 500.000 1250.000 ng/L 0.502 1.179 5.491 21.565 85.380 199.217 514.627 1246.747 %Rec 100.5 94.3 109.8 107.8 85.4 99.6 102.9 99.7 Compound name: PFOSA Coefficient of Determination: RA2 = 0.999179 Calibration curve: 0.000885926 * xA2 + 1.63384 * x + -0.0818517 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: 2nd Order, Origin: Exclude, Weighting: 1/x, Axis trans: None S/N 24 233 362 1027 2887 3310 6334 10787 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 239 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C2-PFOA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.09 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.09 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.14 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.14 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.14 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.14 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.09 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.09 Area 251816 264525 265618 265755 240757 211297 180448 143912 Compound name: 13C2-PFOA Response Factor: 0.948092 RRF SD: 0.0386688, % Relative SD: 4.07859 Response type: Internal Std ( Ref 20 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 92297 97615 93648 93639 85495 75636 60059 47536 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/L 34.532 34.299 35.900 35.922 35.643 35.359 38.028 38.318 %Rec 95.9 95.3 99.7 99.8 99.0 98.2 105.6 106.4 S/N 0 0 0 0 0 0 0 0 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 240 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C4-PFOA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.14 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.09 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.09 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.09 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.09 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.14 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.09 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.09 Compound name: 13C4-PFOA Response Factor: 6728.38 RRF SD: 1520.44, % Relative SD: 22.5974 Response type: External Std, Area Curve type: Rf Area 92297 97615 93648 93639 85495 75636 60059 47536 IS Area Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 13.718 14.508 13.918 13.917 12.707 11.241 8.926 7.065 %Rec 114.3 120.9 116.0 116.0 105.9 93.7 74.4 58.9 S/N 1032 0 409 510 258 731 0 419 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 241 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C4-PFOS-1 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 8.41 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 8.35 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 8.41 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 8.35 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 8.35 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 8.41 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 8.35 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 8.35 Area 13097 14831 14361 14492 14226 11098 8899 6445 Compound name: 13C4-PFOS-1 Response Factor: 0.24213 RRF SD: 0.0283045, % Relative SD: 11.6898 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 29158 30071 31360 31268 30024 25821 23870 18603 Std. Conc 18.000 18.000 18.000 18.000 18.000 18.000 18.000 18.000 ng/L 18.551 20.370 18.913 19.142 19.570 17.751 15.397 14.308 %Rec 103.1 113.2 105.1 106.3 108.7 98.6 85.5 79.5 S/N 423 270 369 474 473 629 193 65 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 242 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C4-PFOS-2 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 8.41 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 8.35 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 8.41 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 8.35 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 8.35 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 8.41 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 8.35 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 8.35 Area 10291 11508 11454 11374 11600 8840 6993 5284 Compound name: 13C4-PFOS-2 Response Factor: 0.192381 RRF SD: 0.0213861, % Relative SD: 11.1165 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 29158 30071 31360 31268 30024 25821 23870 18603 Std. Conc 18.000 18.000 18.000 18.000 18.000 18.000 18.000 18.000 ng/L 18.346 19.892 18.985 18.907 20.083 17.796 15.228 14.764 %Rec 101.9 110.5 105.5 105.0 111.6 98.9 84.6 82.0 S/N 883 283 490 274 932 412 333 251 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 243 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 18O2-PFHxS-1 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.33 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.33 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.33 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.33 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.33 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.33 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.33 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.33 Area 11409 11723 12752 11961 10750 9307 7532 5162 Compound name: 18O2-PFHxS-1 Response Factor: 0.200124 RRF SD: 0.0242557, % Relative SD: 12.1203 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 29158 30071 31360 31268 30024 25821 23870 18603 Std. Conc 18.000 18.000 18.000 18.000 18.000 18.000 18.000 18.000 ng/L 19.552 19.481 20.318 19.115 17.891 18.011 15.767 13.865 %Rec 108.6 108.2 112.9 106.2 99.4 100.1 87.6 77.0 S/N 190 0 0 560 0 3189 2995 0 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 244 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 18O2-PFHxS-2 # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.33 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.33 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.33 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.33 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.33 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.33 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.33 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.33 Area 8167 9543 9276 8817 8538 6193 5239 4007 Compound name: 18O2-PFHxS-2 Response Factor: 0.148217 RRF SD: 0.0207067, % Relative SD: 13.9705 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 29158 30071 31360 31268 30024 25821 23870 18603 Std. Conc 18.000 18.000 18.000 18.000 18.000 18.000 18.000 18.000 ng/L 18.897 21.412 19.957 19.025 19.187 16.181 14.808 14.532 %Rec 105.0 119.0 110.9 105.7 106.6 89.9 82.3 80.7 S/N 1381 0 634 1087 1646 831 0 0 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 245 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C4-PFBA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 5.21 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 5.17 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 5.17 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 5.23 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 5.21 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 5.21 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 5.19 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 5.21 Area 37393 38330 38179 37645 35113 30026 24526 20307 Compound name: 13C4-PFBA Response Factor: 0.982279 RRF SD: 0.0724374, % Relative SD: 7.37442 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 29158 30071 31360 31268 30024 25821 23870 18603 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 13.055 12.977 12.394 12.257 11.906 11.838 10.460 11.113 %Rec 108.8 108.1 103.3 102.1 99.2 98.6 87.2 92.6 S/N 385 137 56 307 247 276 110 125 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM bb MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 246 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C2-PFHxA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 6.36 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 6.34 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 6.36 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 6.36 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 6.34 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 6.36 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 6.33 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 6.34 Area 72936 77481 77682 73854 68145 58258 47890 40957 Compound name: 13C2-PFHxA Response Factor: 1.94279 RRF SD: 0.155326, % Relative SD: 7.99498 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 29158 30071 31360 31268 30024 25821 23870 18603 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 12.875 13.263 12.750 12.157 11.683 11.613 10.327 11.332 %Rec 107.3 110.5 106.3 101.3 97.4 96.8 86.1 94.4 S/N 1873 5235 2508 2940 2714 1874 1928 1008 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 247 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C5-PFNA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 7.55 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 7.49 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 7.49 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 7.55 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 7.55 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 7.55 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 7.49 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.49 Area 76162 83747 83846 83869 79478 71910 55823 45876 Compound name: 13C5-PFNA Response Factor: 2.18642 RRF SD: 0.127832, % Relative SD: 5.8466 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 29158 30071 31360 31268 30024 25821 23870 18603 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 11.946 12.738 12.228 12.268 12.107 12.737 10.696 11.279 %Rec 99.6 106.1 101.9 102.2 100.9 106.1 89.1 94.0 S/N 0 4568 8334 0 0 8344 0 4659 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 248 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C2-PFDA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 8.05 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 8.05 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 8.05 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 8.05 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 8.05 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 8.05 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 8.05 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 7.99 Area 75970 97950 95963 98960 93731 81150 83939 84417 Compound name: 13C2-PFDA Response Factor: 2.75069 RRF SD: 0.466721, % Relative SD: 16.9674 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 29158 30071 31360 31268 30024 25821 23870 18603 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 9.472 11.842 11.124 11.506 11.350 11.425 12.784 16.497 %Rec 78.9 98.7 92.7 95.9 94.6 95.2 106.5 137.5 S/N 716 827 1068 1869 700 619 527 677 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 249 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C2-PFDoA # ID Name Type RT 1 FC-LIN-42420W500-A,, FC3G_015S004 Standard 9.24 2 FC-LIN-42420W500-B,, FC3G_015S005 Standard 9.21 3 FC-LI N-42420W500-C,, FC3G_015S006 Standard 9.24 4 FC-LI N-42420W500-D,, FC3G_015S007 Standard 9.21 5 FC-LIN-42420W500-E,, FC3G_015S008 Standard 9.21 6 FC-LIN-42420W500-F,, FC3G_015S009 Standard 9.24 7 FC-LI N-42420W500-G,, FC3G_015S010 Standard 9.21 8 FC-LIN-42420W500-H,, FC3G_015S011 Standard 9.18 Area 136364 132608 158117 159917 146991 148678 149909 173162 Compound name: 13C2-PFDoA Response Factor: 4.30677 RRF SD: 0.537271, % Relative SD: 12.475 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 29158 30071 31360 31268 30024 25821 23870 18603 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/L 10.859 10.239 11.707 11.875 11.368 13.369 14.582 21.613 %Rec 90.5 85.3 97.6 99.0 94.7 111.4 121.5 180.1 S/N 684 721 867 1318 1065 574 800 882 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MMX PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 250 of 307 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Compound name: 13C2-PFOUEA # ID 1 FC-LIN-42420W500-A,, 2 FC-LIN-42420W500-B,, 3 FC-LI N-42420W500-C,, 4 FC-LI N-42420W500-D,, 5 FC-LIN-42420W500-E,, 6 FC-LIN-42420W500-F,, 7 FC-LI N-42420W500-G,, 8 FC-LIN-42420W500-H,, Name FC3G_015S004 FC3G_015S005 FC3G_015S006 FC3G_015S007 FC3G_015S008 FC3G_015S009 FC3G_015S010 FC3G_015S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.43 7.43 7.38 7.43 7.43 7.43 7.43 7.38 Compound name: 13C2-PFOUEA Response Factor: 2752.2 RRF SD: 447.183, % Relative SD: 16.2482 Response type: External Std, Area Curve type: Rf Area 29158 30071 31360 31268 30024 25821 23870 18603 IS Area Std. Conc 10.000 10.000 10.000 10.000 10.000 10.000 10.000 10.000 ng/L 10.595 10.926 11.395 11.361 10.909 9.382 8.673 6.759 %Rec 105.9 109.3 113.9 113.6 109.1 93.8 86.7 67.6 S/N 4601 0 0 1446 0 2673 9550 2944 Acq.Date 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 14-Jan-13 Acq.Time 20:15:53 20:32:54 20:49:47 21:06:48 21:23:49 21:40:42 21:57:35 22:14:36 Detection... MM MM MM MM MM MM MM MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 251 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time Method: G:\Masslynx\Master Methods\PFC\FC_060_10_01.mdb 03 Dec 2010 17:21:03 Calibration: 15 Jan 2013 10:48:13 ID: FC-LIN-42420W500-A,, Name: FC3G_015S004 Description: 1,,15uL Date: 14-Jan-2013 Time: 20:15:53 PFBA FC3G 015S004 100 5.21 5.21 2.15e3 r 2.15e3 10125 10125 % 1 5.56 7.07 4.03 0 min 5.00 6.00 7.00 PFPeA FC3G 015S004 100- 5.95 2.50e3 10914 %- 5.45 0 - min 5.00 6.00 7.00 PFHxA FC3G_015S004 6.36 100 4.83e3 23057 % 0 min 5.00 6.00 7.00 PFHpA FC3G_015S004 6.73 100 3.75e3 min 5.00 6.00 7.00 PFOA-1 FC3G 015S004 PFNA FC3G 015S004 PFDA FC3G 015S004 PFUnA FC3G 015S004 PFDoA FC3G 015S004 PFBS-1 FC3G 015S004 PFHxS-1 FC3G 015S004 min PFHxS-2 FC3G 015S004 min min PFHxS-3 FC3G 015S004 min min min PFOS-1 FC3G_015S004 8.41 100 1.21e3 3446 % 11.3 1 12.14 PFOS-2 FC3G 015S004 min min 8.00 10.00 12.00 PFOA-2 FC3G 015S004 min min PFBS-2 FC3G_015S004 100- 6.51 7.06e2 3255 %- -4.03 6.16 min 0 min 5.00 6.00 7.00 PFOSA FC3G 015S004 min PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 252 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-A,, Name: FC3G_015S004 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 20:15:53 13C 2 -P FO A FC3G 015S004 13C 4 -P FO A FC3G 015S004 13 C 4 -P FO S-1 FC3G 015S004 13C4-PFBA FC3G 015S004 13C2-PFHXA FC3G 015S004 1 3 C 5 -P F N A FC3G 015S004 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. 13C 4 -P FO S-2 FC3G 015S004 1 3 C 2 -P F D A FC3G 015S004 1 8 0 2 -P FH xS -l FC3G 015S004 1 8 0 2 -P FH X S -2 FC3G 015S004 13C 2-P FD O A FC3G 015S004 min 1 3 C 2 -P FO U E A FC3G 015S004 m Page 253 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN-42420W500-A,, Name: FC3G_015S004 Description: 1,,15uL Date: 14-Jan-2013 Time: 20:15:53 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.21 26 262.98 > 218.... 5.95 26 312.97> 268.98 6.36 26 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 27 462.96 > 418.... 7.55 28 512.96 > 468.... 8.05 28 562.96 > 518.... 8.61 29 612.95 > 568.... 9.24 23 298.94> 79.96 6.51 24 298.94 > 98.95 6.51 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.41 22 498.93> 98.96 8.41 21 498 > 78 9.99 20 414.97 > 369.... 7.09 416.97 > 371.9 7.14 30 503 > 79.96 8.41 30 503 > 98.96 8.41 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.21 30 314.97>269.98 6.36 30 467.96>422.97 7.55 30 514.96 >469.96 8.05 30 614.95 > 569.... 9.24 458.98 >393.98 7.43 Area 2152 2502 4834 3752 4717 1255 3856 5923 5269 6475 1250 706 1177 630 282 1211 650 538 251816 92297 13097 10291 11409 8167 37393 72936 76162 75970 136364 29158 IS Area 37393 72936 72936 72936 251816 251816 76162 75970 75970 136364 11409 8167 11409 8167 11409 13097 10291 13097 92297 29158 29158 29158 29158 29158 29158 29158 29158 29158 ng/L 0.461 0.547 0.469 0.459 0.406 0.570 0.436 0.433 0.439 0.366 0.825 1.157 0.904 1.288 0.863 1.006 0.946 0.502 34.532 13.718 18.551 18.346 19.552 18.897 13.055 12.875 11.946 9.472 10.859 10.595 LOD 0.0135 0.0919 0.0399 0.1409 0.0656 0.3215 0.0408 0.1170 0.0254 0.0376 0.0551 0.0069 %Rec 92.2 109.3 93.8 91.8 81.1 113.9 87.2 86.5 87.9 73.2 82.5 115.7 90.4 128.8 86.3 100.6 94.6 100.5 95.9 114.3 103.1 101.9 108.6 105.0 108.8 107.3 99.6 78.9 90.5 105.9 S/N 10 50 142 105 39 77 43 89 74 111 141 67 0 89 25 34 37 24 0 1032 423 883 190 1381 385 1873 0 716 684 4601 Noise 990 220 162 152 519 65 340 242 298 251 36 49 0 31 36 102 71 68 0 355 126 47 238 22 426 183 0 384 880 24 Height 10125 10914 23057 16082 20192 5041 14554 21436 22052 28001 5046 3255 4505 2752 886 3446 2630 1628 1010883 366986 53492 41289 45336 30979 163796 342470 288608 274796 601392 111169 IS Height 163796 342470 342470 342470 1010883 1010883 288608 274796 274796 601392 45336 30979 45336 30979 45336 53492 41289 53492 366986 111169 111169 111169 111169 111169 111169 111169 111169 111169 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 254 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-B,, Name: FC3G_015S005 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 20:32:54 PFBA FC3G 015S005 PFPeA FC3G 015S005 PFHxA FC3G 015S005 PFNA FC3G 015S005 PFDA FC3G 015S005 PFUnA FC3G 015S005 P F H xS -1 FC3G_015S005 - 7 77 100 ]2 .7 6 e 3 7 '\ 10898 / \ P F H xS -2 FC3G 015S005 1 0 0 - 1 ,2 6 e 3 7 \ 4234 %\ P F H xS -3 FC3G 015S005 1 0 0 - 6 .2 5 e 2 7 \ 1987 \ %1 ii iiU iii Ii nrrrr i min 7.00 8.00 o- i i i i iii i i r i min 7.00 8.00 0- 7.00 8.00 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. PFHpA FC3G 015S005 PFOA-1 FC3G 015S005 PFOA-2 FC3G 015S005 PFDoA FC3G 015S005 PFBS-1 FC3G 015S005 P F B S -2 FC3G 015S005 P FO S-1 FC3G 015S005 P FO S-2 FC3G 015S005 PFO SA FC3G 015S005 m Page 255 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-B,, Name: FC3G_015S005 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 20:32:54 13C 2 -P FO A 13C 4 -P FO A 13 C 4 -P FO S-1 13C4-PFBA FC3G_015S005 100n 5.17 3.83e4 143553 -4.09 tt min 5.00 6.00 7.00 13C2-PFHXA FC3G 015S005 100- 6.34 7.75e4 342906 %- o- iiintimi min 5.00 6.00 7.00 1 3 C 5 -P F N A FC3G_015S005 100 7.49 8.37e4 327169 % 0 7.00 8.00 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. 13C 4 -P FO S-2 FC3G_015S005 100- 8.35 1.15e4 44535 1 8 0 2 -P FH xS -l FC3G 015S005 8.00 10.00 12.00 1 3 C 2 -P F D A 13C 2-P FD O A 1 8 0 2 -P FH X S -2 FC3G 015S005 1 3 C 2 -P FO U E A m Page 256 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN-42420W500-B,, Name: FC3G_015S005 Description: 1,,15uL Date: 14-Jan-2013 Time: 20:32:54 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.19 26 262.98 > 218.... 5.95 26 312.97> 268.98 6.34 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 > 418.... 7.55 28 512.96 > 468.... 8.05 28 562.96 > 518.... 8.61 29 612.95 > 568.... 9.24 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.49 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.41 22 498.93> 98.96 8.41 21 498 > 78 9.99 20 414.97 > 369.... 7.09 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.17 30 314.97>269.98 6.34 30 467.96>422.97 7.49 30 514.96 >469.96 8.05 30 614.95 > 569.... 9.21 458.98 >393.98 7.43 Area 5517 6331 11980 10472 12440 2315 10365 14790 14561 15981 2925 1423 2758 1261 625 2764 1840 1521 264525 97615 14831 11508 11723 9543 38330 77481 83747 97950 132608 30071 IS Area 38330 77481 77481 77481 264525 264525 83747 97950 97950 132608 11723 9543 11723 9543 11723 14831 11508 14831 97615 30071 30071 30071 30071 30071 30071 30071 30071 30071 ng/L 1.442 1.361 1.402 1.325 1.416 1.124 1.338 1.246 1.247 1.374 2.636 2.223 2.798 2.274 2.208 2.605 2.777 1.179 34.299 14.508 20.370 19.892 19.481 21.412 12.977 13.263 12.738 11.842 10.239 10.926 LOD 0.0642 0.2371 0.2163 0.2821 0.0089 0.0087 0.0403 0.0492 %Rec 115.3 108.9 112.1 106.0 113.3 90.0 107.0 99.7 99.8 109.9 105.4 88.9 111.9 90.9 88.3 104.2 111.1 94.3 95.3 120.9 113.2 110.5 108.2 119.0 108.1 110.5 106.1 98.7 85.3 109.3 S/N 48 141 213 189 74 83 144 377 240 224 544 198 0 0 51 44 78 233 0 0 270 283 0 0 137 5235 4568 827 721 0 Noise 408 189 248 217 656 95 265 142 267 296 23 30 0 0 39 180 73 25 0 0 217 157 0 0 1048 65 72 419 801 0 Height 19656 26769 52648 41080 48425 7853 38135 53536 64070 66499 12582 6029 10898 4234 1987 8004 5707 5815 1043751 393569 58666 44535 46481 37907 143553 342906 327169 346543 577699 113503 IS Height 143553 342906 342906 342906 1043751 1043751 327169 346543 346543 577699 46481 37907 46481 37907 46481 58666 44535 58666 393569 113503 113503 113503 113503 113503 113503 113503 113503 113503 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 257 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-C,, Name: FC3G_015S006 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 20:49:47 PFBA FC3G 015S006 100- 5.19 1.77e4 72128 %- l/ 7.07 5.00 6.00 7.00 PFPeA FC3G 015S006 PFNA FC3G 015S006 PFDA FC3G 015S006 PFHxA FC3G 015S006 PFUnA FC3G 015S006 P F H xS -1 FC3G 015S006 P F H xS -2 FC3G 015S006 P F H xS -3 FC3G 015S006 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. PFHpA FC3G 015S006 PFOA-1 FC3G 015S006 P F O A -2 FC3G 015S006 PFDoA FC3G 015S006 PFBS-1 FC3G 015S006 P F B S -2 FC3G 015S006 P FO S-1 FC3G 015S006 P FO S-2 FC3G 015S006 PFO SA FC3G 015S006 m Page 258 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-C,, Name: FC3G_015S006 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 20:49:47 13C 2 -P FO A FC3G_015S006 100n 7.14 2.66e5 1056061 13C 4 -P FO A FC3G 015S006 100- 7.09 f\ 9.36e4 \368880 %- 13 C 4 -P FO S-1 FC3G_015S006 100 8.41 % -r min o- --i r min 0 7.00 8.00 7.00 8.00 8.00 10.00 12.00 13C4-PFBA FC3G_015S006 100n 5.17 3.82e4 157004 -4.07 tt min 5.00 6.00 7.00 13C2-PFHXA FC3G 015S006 100- 6.36 7.77e4~i 355924 %- j o- 11mtti11 min 5.00 6.00 7.00 1 3 C 5 -P F N A FC3G_015S006 100 7.49 8.38e4 324037 % 0 7.00 8.00 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. 13C4-PFOS-2 FC3G 015S006 1 8 0 2 -P FH xS -l FC3G 015S006 1 8 0 2 -P FH X S -2 FC3G 015S006 1 3 C 2 -P F D A FC3G 015S006 13C 2-P FD O A FC3G 015S006 1 3 C 2 -P FO U E A FC3G 015S006 m Page 259 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN-42420W500-C,, Name: FC3G_015S006 Description: 1,,15uL Date: 14-Jan-2013 Time: 20:49:47 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.19 26 262.98 > 218.... 5.97 26 312.97> 268.98 6.36 26 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.09 27 462.96 > 418.... 7.55 28 512.96 > 468.... 8.05 28 562.96 > 518.... 8.61 29 612.95 > 568.... 9.24 23 298.94> 79.96 6.49 24 298.94 > 98.95 6.49 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.41 22 498.93> 98.96 8.41 21 498 > 78 9.99 20 414.97 > 369.... 7.14 416.97 > 371.9 7.09 30 503 > 79.96 8.41 30 503 > 98.96 8.41 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.17 30 314.97>269.98 6.36 30 467.96>422.97 7.49 30 514.96 >469.96 8.05 30 614.95 > 569.... 9.24 458.98 >393.98 7.38 Area 17665 20792 36978 39072 40626 9021 36435 48705 51692 64757 10293 5529 8511 4912 3012 9055 6360 7114 265618 93648 14361 11454 12752 9276 38179 77682 83846 95963 158117 31360 IS Area 38179 77682 77682 77682 265618 265618 83846 95963 95963 158117 12752 9276 12752 9276 12752 14361 11454 14361 93648 31360 31360 31360 31360 31360 31360 31360 31360 31360 ng/L 5.062 4.557 4.796 5.132 5.199 4.835 5.169 5.225 5.224 5.370 9.852 9.821 8.991 9.407 10.815 10.163 10.251 5.491 35.900 13.918 18.913 18.985 20.318 19.957 12.394 12.750 12.228 11.124 11.707 11.395 LOD 0.0044 0.0902 0.1022 0.1579 0.1218 0.1030 0.7367 0.0190 0.0046 0.0308 0.0457 %Rec 101.2 91.1 95.9 102.6 104.0 96.7 103.4 104.5 104.5 107.4 98.5 98.2 89.9 94.1 108.2 101.6 102.5 109.8 99.7 116.0 105.1 105.5 112.9 110.9 103.3 106.3 101.9 92.7 97.6 113.9 S/N 377 429 688 1062 0 169 578 356 724 717 2250 717 139 0 102 126 131 362 0 409 369 490 0 634 56 2508 8334 1068 867 0 Noise 191 219 248 152 0 205 237 499 313 378 20 37 203 0 112 212 159 66 0 903 147 90 0 59 2783 142 39 325 757 0 Height 72128 94191 170385 161879 160252 34563 137190 177519 226802 271378 44055 26571 28190 18280 11385 26702 20746 23880 1056061 368880 54308 44158 51584 37197 157004 355924 324037 347325 656524 115363 IS Height 157004 355924 355924 355924 1056061 1056061 324037 347325 347325 656524 51584 37197 51584 37197 51584 54308 44158 54308 368880 115363 115363 115363 115363 115363 115363 115363 115363 115363 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 260 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-D,, Name: FC3G_015S007 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 21:06:48 PFBA FC3G 015S007 PFPeA FC3G 015S007 PFHxA FC3G 015S007 PFNA FC3G 015S007 PFDA FC3G 015S007 PFUnA FC3G 015S007 P F H xS -1 FC3G 015S007 P F H xS -2 FC3G 015S007 P F H xS -3 FC3G 015S007 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. PFHpA FC3G 015S007 PFOA-1 FC3G 015S007 P F O A -2 FC3G 015S007 PFDoA FC3G 015S007 PFBS-1 FC3G 015S007 P F B S -2 FC3G 015S007 P FO S-1 FC3G 015S007 P FO S-2 FC3G 015S007 PFO SA FC3G_015S007 100- 9.99 2.86e4 99782 %- 8.00 10.00 12.00 m Page 261 of 307 w w w .axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-D,, Name: FC3G_015S007 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 21:06:48 13C 2 -P FO A FC3G 015S007 13C 4 -P FO A FC3G 015S007 13 C 4 -P FO S-1 FC3G_015S007 100- 8.35 1,45e4 55563 13C4-PFBA FC3G 015S007 13C2-PFHXA FC3G 015S007 8.00 10.00 12.00 1 3 C 5 -P F N A FC3G 015S007 P V by JU LI, S V by K JC 4 -J a n -1 3. C a lv e r co n e in ng. 13C4-PFOS-2 FC3G 015S007 1 8 0 2 -P FH xS -l FC3G 015S007 1 8 0 2 -P FH X S -2 FC3G 015S007 1 3 C 2 -P F D A FC3G 015S007 13C 2-P FD O A FC3G 015S007 1 3 C 2 -P FO U E A FC3G 015S007 m Page 262 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN-42420W500-D,, Name: FC3G_015S007 Description: 1,,15uL Date: 14-Jan-2013 Time: 21:06:48 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.23 26 262.98 > 218.... 5.95 26 312.97> 268.98 6.34 26 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 27 462.96 > 418.... 7.55 28 512.96 > 468.... 8.05 28 562.96 > 518.... 8.61 29 612.95 > 568.... 9.21 23 298.94> 79.96 6.49 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.41 22 498.93> 98.96 8.35 21 498 > 78 9.99 20 414.97 > 369.... 7.14 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.23 30 314.97>269.98 6.36 30 467.96>422.97 7.55 30 514.96 >469.96 8.05 30 614.95 > 569.... 9.21 458.98 >393.98 7.43 Area 63528 80737 140318 144182 149341 34651 144493 185619 199149 244382 41241 20643 35764 17711 10989 33164 22836 28634 265755 93639 14492 11374 11961 8817 37645 73854 83869 98960 159917 31268 IS Area 37645 73854 73854 73854 265755 265755 83869 98960 98960 159917 11961 8817 11961 8817 11961 14492 11374 14492 93639 31268 31268 31268 31268 31268 31268 31268 31268 31268 ng/L 18.984 18.749 19.866 20.144 19.811 19.037 21.027 20.576 20.315 20.934 44.008 39.540 42.269 35.989 42.899 38.306 37.683 21.565 35.922 13.917 19.142 18.907 19.115 19.025 12.257 12.157 12.268 11.506 11.875 11.361 LOD 0.0766 0.1079 0.0819 0.1218 0.2051 0.1075 0.0564 0.1320 0.0155 0.0178 0.0312 0.0236 %Rec 94.9 93.7 99.3 100.7 99.1 95.2 105.1 102.9 101.6 104.7 110.0 98.8 105.7 90.0 107.2 95.8 94.2 107.8 99.8 116.0 106.3 105.0 106.2 105.7 102.1 101.3 102.2 95.9 99.0 113.6 S/N 575 1512 6800 3803 0 1499 13918 1253 1495 2177 2292 4112 281 3728 0 522 314 1027 0 510 474 274 560 1087 307 2940 0 1869 1318 1446 Noise 482 254 102 156 0 87 39 552 589 478 86 22 463 17 0 167 246 97 0 740 117 157 86 33 515 120 0 195 533 82 Height 277157 383441 695418 593000 609477 130403 540922 691429 881123 1040393 196622 89957 130257 64214 42122 87281 77361 99782 1072138 377462 55563 42980 47904 36129 158352 351583 326064 363683 703192 119249 IS Height 158352 351583 351583 351583 1072138 1072138 326064 363683 363683 703192 47904 36129 47904 36129 47904 55563 42980 55563 377462 119249 119249 119249 119249 119249 119249 119249 119249 119249 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 263 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-E,, Name: FC3G_015S008 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 21:23:49 PFBA FC3G 015S008 PFPeA FC3G 015S008 PFHxA FC3G 015S008 PFNA FC3G 015S008 PFDA FC3G 015S008 PFUnA FC3G 015S008 P F H xS -1 FC3G 015S008 P F H xS -2 FC3G 015S008 P F H xS -3 FC3G 015S008 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. PFHpA FC3G 015S008 PFOA-1 FC3G 015S008 P F O A -2 FC3G 015S008 PFDoA FC3G 015S008 PFBS-1 FC3G 015S008 P F B S -2 FC3G 015S008 P FO S-1 FC3G 015S008 P FO S-2 FC3G 015S008 PFO SA FC3G 015S008 m Page 264 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-E,, Name: FC3G_015S008 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 21:23:49 13C 2 -P FO A FC3G_015S008 100n 7.14 2.41e5 957584 T-r min 7.00 8.00 13C 4 -P FO A FC3G 015S008 100- 7.09 IT 8.55e4 J V,I \343729 %- o- --i r min 7.00 8.00 13 C 4 -P FO S-1 FC3G_015S008 100 8.35 1.42e4 54947 % 0 8.00 10.00 12.00 13C4-PFBA FC3G 015S008 13C2-PFHXA FC3G 015S008 1 3 C 5 -P F N A FC3G 015S008 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. 13C 4 -P FO S-2 1 8 0 2 -P FH xS -l 1 8 0 2 -P FH X S -2 1 3 C 2 -P F D A 13C 2-P FD O A 1 3 C 2 -P FO U E A m Page 265 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN-42420W500-E,, Name: FC3G_015S008 Description: 1,,15uL Date: 14-Jan-2013 Time: 21:23:49 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.24 26 262.98 > 218.... 5.97 26 312.97> 268.98 6.36 26 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 27 462.96 > 418.... 7.55 28 512.96 > 468.... 8.05 28 562.96 > 518.... 8.61 29 612.95 > 568.... 9.21 23 298.94> 79.96 6.49 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.35 22 498.93> 98.96 8.35 21 498 > 78 9.99 20 414.97 > 369.... 7.14 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.21 30 314.97>269.98 6.34 30 467.96>422.97 7.55 30 514.96 >469.96 8.05 30 614.95 > 569.... 9.21 458.98 >393.98 7.43 Area 301419 379493 632521 638236 692014 171424 643515 879693 946327 1081028 176606 93835 152325 86045 51386 162731 118969 115291 240757 85495 14226 11600 10750 8538 35113 68145 79478 93731 146991 30024 IS Area 35113 68145 68145 68145 240757 240757 79478 93731 93731 146991 10750 8538 10750 8538 10750 14226 11600 14226 85495 30024 30024 30024 30024 30024 30024 30024 30024 30024 ng/L 97.717 95.772 98.807 97.204 102.573 105.039 98.921 107.421 104.927 104.702 211.633 187.968 202.336 181.967 223.279 191.828 192.444 85.380 35.643 12.707 19.570 20.083 17.891 19.187 11.906 11.683 12.107 11.350 11.368 10.909 LOD 0.0579 0.1299 0.1480 0.1310 0.0720 0.0349 0.1484 0.0173 0.0462 0.0366 %Rec 97.7 95.8 98.8 97.2 102.6 105.0 98.9 107.4 104.9 104.7 105.8 94.0 101.2 91.0 111.6 95.9 96.2 85.4 99.0 105.9 108.7 111.6 99.4 106.6 99.2 97.4 100.9 94.6 94.7 109.1 S/N 6913 8374 15254 12076 0 0 0 4480 4936 3889 36944 8405 0 0 0 1675 1585 2887 0 258 473 932 0 1646 247 2714 0 700 1065 0 Noise 160 206 193 208 0 0 0 685 799 1144 19 49 0 0 0 272 255 133 0 1334 116 51 0 19 534 123 0 465 577 0 Height 1108375 1728053 2947189 2509939 2750171 682666 2418926 3067496 3943127 4450521 708659 408264 543837 321200 180229 456383 404127 383237 957584 343729 54947 47235 41229 31148 131545 334627 307047 325898 614569 109805 IS Height 131545 334627 334627 334627 957584 957584 307047 325898 325898 614569 41229 31148 41229 31148 41229 54947 47235 54947 343729 109805 109805 109805 109805 109805 109805 109805 109805 109805 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 266 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-F,, Name: FC3G_015S009 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 21:40:42 PFBA FC3G 015S009 PFPeA FC3G 015S009 PFHxA FC3G 015S009 PFNA FC3G 015S009 100- 7.55 1.15e6 4232489 %- \\ 7.00 8.00 P F H xS -1 FC3G 015S009 PFDA FC3G 015S009 100- 8.05 F 1.42e6 5162865 %- PFUnA FC3G_015S009 100- 8.64 1.62e6 | 6976139 %- 'i i1I iViTi r| i i i i | i i iT-p-n-rp-r min 8.00 10.00 12.00 i|ih 8.00 10.00 12.00 P F H xS -2 FC3G 015S009 P F H xS -3 FC3G 015S009 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. PFHpA FC3G 015S009 100- 6.73 1.14e6 1 4502942 I1%- 5.00 6.00 7.00 PFDoA FC3G 015S009 PFOA-1 FC3G 015S009 100- 7.14 1,20e6 4803563 %- iJ V , 7.00 8.00 PFBS-1 FC3G 015S009 PFOA-2 FC3G 015S009 100- 7.14 2.86e5 1172249 %- iJ 7.00 8.00 P F B S -2 FC3G 015S009 P FO S-1 FC3G 015S009 P FO S-2 FC3G 015S009 PFO SA FC3G 015S009 m Page 267 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-F,, Name: FC3G_015S009 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 21:40:42 13C 2 -P FO A FC3G_015S009 100n 7.14 2 .1 1e5 846753 T-r min 7.00 8.00 13C 4 -P FO A FC3G 015S009 100- 7.14 FT 7.56e4 \ 304003 %- IV o7.00 -T-r min 8.00 13 C 4 -P FO S-1 FC3G_015S009 100 8.41 % 0 8.00 10.00 12.00 13C4-PFBA FC3G 015S009 13C2-PFHXA FC3G 015S009 1 3 C 5 -P F N A FC3G 015S009 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. 13C4-PFOS-2 FC3G 015S009 1 8 0 2 -P FH xS -l FC3G 015S009 1 8 0 2 -P FH X S -2 FC3G 015S009 1 3 C 2 -P F D A FC3G 015S009 13C 2-P FD O A FC3G 015S009 1 3 C 2 -P FO U E A FC3G 015S009 m Page 268 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN-42420W500-F,, Name: FC3G_015S009 Description: 1,,15uL Date: 14-Jan-2013 Time: 21:40:42 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.23 26 262.98 > 218.... 5.97 26 312.97> 268.98 6.36 26 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 27 462.96 > 418.... 7.55 28 512.96 > 468.... 8.05 28 562.96 > 518.... 8.64 29 612.95 > 568.... 9.24 23 298.94> 79.96 6.49 24 298.94 > 98.95 6.51 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.41 22 498.93> 98.96 8.41 21 498 > 78 9.99 20 414.97 > 369.... 7.14 416.97 > 371.9 7.14 30 503 > 79.96 8.41 30 503 > 98.96 8.41 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.21 30 314.97>269.98 6.36 30 467.96>422.97 7.55 30 514.96 >469.96 8.05 30 614.95 > 569.... 9.24 458.98 >393.98 7.43 Area 500633 670319 1071639 1139370 1197420 286450 1146232 1420377 1622024 2106076 287793 150714 267940 141803 82734 271377 190358 222311 211297 75636 11098 8840 9307 6193 30026 58258 71910 81150 148678 25821 IS Area 30026 58258 58258 58258 211297 211297 71910 81150 81150 148678 9307 6193 9307 6193 9307 11098 8840 11098 75636 25821 25821 25821 25821 25821 25821 25821 25821 25821 ng/L 190.827 198.161 198.222 203.894 202.861 200.887 193.521 206.864 213.112 209.250 398.208 420.913 411.296 417.389 413.136 405.150 401.314 199.217 35.359 11.241 17.751 17.796 18.011 16.181 11.838 11.613 12.737 11.425 13.369 9.382 LOD 118.9377 0.2008 0.3825 0.2250 0.0461 0.0820 0.1182 0.0161 0.0518 0.1242 0.0229 0.0044 0.0517 0.0720 0.0105 %Rec 95.4 99.1 99.1 101.9 101.4 100.4 96.8 103.4 106.6 104.6 99.6 105.2 102.8 104.3 103.3 101.3 100.3 99.6 98.2 93.7 98.6 98.9 100.1 89.9 98.6 96.8 106.1 95.2 111.4 93.8 S/N 5 16850 26475 27466 0 0 22371 4875 8175 11005 15577 18009 10903 4240 4172 1808 1716 3310 0 731 629 412 3189 831 276 1874 8344 619 574 2673 Noise 393438 175 196 164 0 0 189 1059 853 815 75 34 84 118 68 412 350 222 0 416 67 76 11 26 410 150 32 478 1042 38 Height 1855019 2956717 5190074 4502942 4803563 1172249 4232489 5162865 6976139 8964105 1167220 610202 911714 501759 285200 745767 600727 735982 846753 304003 42004 31488 34584 21433 113120 280566 269017 295754 597960 100842 IS Height 113120 280566 280566 280566 846753 846753 269017 295754 295754 597960 34584 21433 34584 21433 34584 42004 31488 42004 304003 100842 100842 100842 100842 100842 100842 100842 100842 100842 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 269 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-G,, Name: FC3G_015S010 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 21:57:35 PFBA FC3G 015S010 PFPeA FC3G 015S010 PFHxA FC3G 015S010 PFNA FC3G 015S010 PFDA FC3G 015S010 PFUnA FC3G 015S010 P F H xS -1 FC3G 015S010 P F H xS -2 FC3G 015S010 P F H xS -3 FC3G 015S010 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. PFHpA FC3G 015S010 PFOA-1 FC3G 015S010 PFOA-2 FC3G 015S010 PFDoA FC3G_015S010 100- 9.21 4.34e6 18023478 %- PFBS-1 FC3G 015S010 P F B S -2 FC3G 015S010 8.00 10.00 12.00 P FO S-1 FC3G 015S010 100- 8.35 r 5.61e5 1368011 %- V 9?9 8.00 10.00 12.00 P FO S-2 FC3G_015S010 100 8.35 3.88e5 1194386 % 0 8.00 10.00 12.00 PFO SA FC3G 015S010 m Page 270 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-G,, Name: FC3G_015S010 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 21:57:35 13C 2 -P FO A FC3G 015S010 13C 4 -P FO A FC3G 015S010 13 C 4 -P FO S-1 FC3G 015S010 13C4-PFBA FC3G 015S010 13C2-PFHXA FC3G 015S010 1 3 C 5 -P F N A FC3G 015S010 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. 13C 4 -P FO S-2 FC3G 015S010 100- 8.35 6.99e3 22920 %- -si cccCoonD^ccoo 1 8 0 2 -P FH xS -l FC3G 015S010 100- 26355 %- t i' i' i i' i' i i r min 8.00 10.00 12.00 'll 7.00 8.00 1 3 C 2 -P F D A FC3G 015S010 13C 2-P FD O A FC3G 015S010 1 8 0 2 -P FH X S -2 FC3G 015S010 100- 7.33 5.24e3 18010 %- ' I' PiJ 7.00 8.00 1 3 C 2 -P FO U E A FC3G 015S010 min m Page 271 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN-42420W500-G,, Name: FC3G_015S010 Description: 1,,15uL Date: 14-Jan-2013 Time: 21:57:35 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.23 26 262.98 > 218.... 5.95 26 312.97> 268.98 6.33 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 > 418.... 7.49 28 512.96 > 468.... 8.05 28 562.96 > 518.... 8.61 29 612.95 > 568.... 9.21 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.35 22 498.93> 98.96 8.35 21 498 > 78 9.99 20 414.97 > 369.... 7.09 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.19 30 314.97>269.98 6.33 30 467.96>422.97 7.49 30 514.96 >469.96 8.05 30 614.95 > 569.... 9.21 458.98 >393.98 7.43 Area 1092578 1417364 2165990 2262452 2464122 592967 2391985 3073619 3473490 4341857 574749 292314 516587 284063 155131 560878 387780 531646 180448 60059 8899 6993 7532 5239 24526 47890 55823 83939 149909 23870 IS Area 24526 47890 47890 47890 180448 180448 55823 83939 83939 149909 7532 5239 7532 5239 7532 8899 6993 8899 60059 23870 23870 23870 23870 23870 23870 23870 23870 23870 ng/L 518.258 511.694 505.086 498.311 491.806 492.779 508.363 471.682 468.725 470.764 978.718 990.315 978.341 1012.035 942.917 1006.719 1012.010 514.627 38.028 8.926 15.397 15.228 15.767 14.808 10.460 10.327 10.696 12.784 14.582 8.673 LOD 0.0005 0.0132 0.2808 0.1341 1.1128 0.3674 0.2142 0.1213 0.0149 0.2999 0.0216 0.0724 0.0624 0.0027 %Rec 103.7 102.3 101.0 99.7 98.4 98.6 101.7 94.3 93.7 94.2 97.9 99.0 97.8 101.2 94.3 100.7 101.2 102.9 105.6 74.4 85.5 84.6 87.6 82.3 87.2 86.1 89.1 106.5 121.5 86.7 S/N 7187 22502 49340 53152 0 0 36654 8972 9193 9659 76153 31388 0 7540 0 3373 2172 6334 0 0 193 333 2995 0 110 1928 0 527 800 9550 Noise 543 259 216 159 0 0 245 1262 1586 1866 29 35 0 126 0 406 550 283 0 0 153 69 9 0 870 124 0 588 794 9 Height IS Height 3900374 95829 5833103 238596 10668906 238596 8433429 238596 10217171 751674 2491062 751674 8991793 211667 11320552 310075 14579862 310075 18023478 634663 2238738 26355 1113917 18010 1718023 26355 951715 18010 486245 26355 1368011 29511 1194386 22920 1794902 29511 751674 246167 246167 29511 88586 22920 88586 26355 88586 18010 88586 95829 88586 238596 88586 211667 88586 310075 88586 634663 88586 88586 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 272 of 307 w w w .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN-42420W500-H,, Name: FC3G_015S011 Description: 1,,15uL Date: 14-Jan-2013 Time: 22:14:36 PFBA FC3G 015S011 PFPeA FC3G 015S011 PFHxA FC3G 015S011 PFNA FC3G 015S011 100- 7.49 5.09e6 V18762150 %- 7.00 PFHxS-1 FC3G 015S011 8.00 PFDA FC3G 015S011 100- 8.05 (T 6.10e6 I 20749386 %- PFUnA FC3G_015S011 100 8.58 7.62e6 30035668 % i1/i I iiTtTi i i i i i i i i i i i i i i i mm 8.00 10.00 12.00 0 8.00 10.00 12.00 PFHxS-2 FC3G 015S011 PFHxS-3 FC3G 015S011 P V by JU LI, S V by K JC 4 -J a n -1 3. C a lv e r co n e in ng. PFHpA FC3G 015S011 PFOA-1 FC3G 015S011 P F O A -2 FC3G 015S011 PFDoA FC3G 015S011 PFBS-1 FC3G 015S011 P F B S -2 FC3G 015S011 P FO S-1 FC3G 015S011 100- 8.35 P 1.10e6 2514590 %- Vi 9^ 9 8.00 10.00 12.00 P FO S-2 FC3G_015S011 100 8.35 ,60e5 % 0 8.00 10.00 12.00 PFO SA FC3G 015S011 m Page 273 of 307 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN -42420W 500-H,, Name: FC3G_015S011 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 22:14:36 13C 2 -P FO A FC3G 015S011 13C 4 -P FO A FC3G 015S011 13 C 4 -P FO S-1 FC3G 015S011 13C4-PFBA FC3G 015S011 13C2-PFHXA FC3G 015S011 1 3 C 5 -P F N A FC3G 015S011 PV by JULI, SV by KJC 4-Jan-13. Calver cone in ng. 13C4-PFOS-2 FC3G 015S011 1 8 0 2 -P FH xS -l FC3G 015S011 1 8 0 2 -P FH X S -2 FC3G 015S011 1 3 C 2 -P F D A FC3G 015S011 13C 2-P FD O A FC3G 015S011 1 3 C 2 -P FO U E A FC3G 015S011 m Page 274 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_015-A1.qld Last Altered: Tuesday, January 15, 2013 10:48:13 Pacific Standard Time Printed: Tuesday, January 15, 2013 11:33:51 Pacific Standard Time ID: FC-LIN-42420W500-H,, Name: FC3G_015S011 Description: 1,,15uL Date: 14-Jan-2013 Time: 22:14:36 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 18O2-PFHxS-1 24 18O2-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.24 26 262.98 > 218.... 5.97 26 312.97> 268.98 6.34 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 > 418.... 7.49 28 512.96 > 468.... 8.05 28 562.96 > 518.... 8.58 29 612.95 > 568.... 9.18 23 298.94> 79.96 6.49 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.35 22 498.93> 98.96 8.35 21 498 > 78 9.99 20 414.97 > 369.... 7.09 416.97 > 371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.21 30 314.97>269.98 6.34 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.18 458.98 >393.98 7.38 Area 2094684 2925513 4181689 4711523 4933775 1165768 5089518 6104199 7620483 9446096 1021844 526024 912450 503432 296727 1099194 759759 1222304 143912 47536 6445 5284 5162 4007 20307 40957 45876 84417 173162 18603 IS Area 20307 40957 40957 40957 143912 143912 45876 84417 84417 173162 5162 4007 5162 4007 5162 6445 5284 6445 47536 18603 18603 18603 18603 18603 18603 18603 18603 18603 ng/L 1243.976 1245.910 1248.015 1250.282 1252.684 1252.495 1247.932 1274.461 1266.699 1285.038 2507.622 2501.618 2506.565 2493.009 2517.457 2497.579 2496.000 1246.747 38.318 7.065 14.308 14.764 13.865 14.532 11.113 11.332 11.279 16.497 21.613 6.759 LOD 662.9949 0.0140 0.2056 0.1181 1.1581 1.4137 0.6197 0.0506 0.5707 0.1620 0.2414 0.0468 0.0072 0.0713 0.0745 0.0069 %Rec 99.5 99.7 99.8 100.0 100.2 100.2 99.8 102.0 101.3 102.8 100.3 100.1 100.3 99.7 100.7 99.9 99.8 99.7 106.4 58.9 79.5 82.0 77.0 80.7 92.6 94.4 94.0 137.5 180.1 67.6 S/N 5 50663 50289 91806 0 0 0 3540 7802 11577 48512 51919 0 0 0 3626 4089 10787 0 419 65 251 0 0 125 1008 4659 677 882 2944 Noise 1290144 232 416 190 0 0 0 5861 3850 3045 76 36 0 0 0 694 526 354 0 469 318 72 0 0 545 209 36 451 738 23 Height IS Height 7055448 68400 11775853 210699 20933800 210699 17456350 210699 20590308 605737 4844712 605737 18762150 169750 20749386 305304 30035668 305304 35251636 650347 3668703 17840 1877622 14336 2831564 17840 1639389 14336 934732 17840 2514590 20527 2150387 17955 3817710 20527 605737 196532 196532 20527 68997 17955 68997 17840 68997 14336 68997 68400 68997 210699 68997 169750 68997 305304 68997 650347 68997 68997 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMX 1.0 MM PV by JLIU, SV by KJC 4-Jan-13. Calver conc in ng. Page 275 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4.1 SCN627 D a ta se t: G :\M asslynx\lnst_G \PFC .P R O \FC \FC 3G ..01 5-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Tim e Printed: W ednesday, January 16, 2013 11:54:43 Pacific Standard Tim e ID: FC-L1N-42420W500-D,, Name: FC3G_015S016 Description: 1,,15uL Date: 14-Jan-2013 Time: 23:39:26 PFBA FC3G_015S016 loo- I1" 265c.0683e643 ~ m in 5.00 6.00 7.00 PFPeA FC3G 015S016 io o -q 376.7533e249 I j %- I ;; ; 0\ - -------- r n i n 5.00 6.00 7.00 PFHxA FC3G 015S016 100- 618.4123e255"p1 %~ : . 0 5.00 6.00 7.00 PFHpA FC3G 015S016 100- 516.3794e8551 %- ;; 0- -- ---------------- -- m jn 5.00 6.00 7.00 PFOA-1 FC3G 015S016 1007! A 1.52e 5 ! \605973 % 0 / x\ . 7.00 . 8.00 PFNA 3F C G 015S 016 PFDA F C 3G _ 015S 016 7.99 100-1 F 1.80e 5 I 651089 %I ' f\ 0 V f j l PTi' i i I 8.00 10.00 -rprr fn!n 12.00 PFUnA F C 3G _ 015S 016 in n 100^ r I 8178-97-5238e655 %~ PFDoA 3F C G 015S 016 100- 9.18 F 2.24e 5 i| 940818 %- A Tjn min 8.00 10.00 12.00 0- 8.00 t t m in 10.00 12.00 PFBS-1 3F C G 015S 016 100- 6.47 4.21e 4 ; 197084 i. %- A 0- --------------------------- ........m i n 5.00 6.00 7.00 PFHxS-1 3F C G 015S 016 PFHxS-2 F C 3G__015S 016 7.33 100-~1. 95e 4 A 74740j \ %\ PFHxS-3 FC3G__015S016 7.33 100--1 .0 4 e 4 l| ; 38961 m %- PFOS-1 3F C G 015S 016 PFOS-2 F C 3G__015S 016 100- _ 8.35 2.23e 4 73451 8.00 7.00 8.00 8.00 10.00 12.00 PFOA-2 FC3G_015S016 100- j 7.09 3.65e4 u 148035 %- 07.00 8.00 PFBS-2 3F C G 015S 016 100- 6.47 2.08e 4 91030 ;i % M :\ o- ; ^ 5.00 6.00 7,00 PFOSA F C 3G _ 015S 016 9.96 100- 2.33e 4 l 79478 H %- /: 8.00 7JTT min 10.00 12.00 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/m L, Sam ple and Lab BLK Gonen. & LOD units = ng/L. Page 276 of 307 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnstJ3\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: FC-LIN-42420W500-D,, Name: FC3G_015S016 Description: 1,,15uL Date: 14^Jan-2013 Time: 23:39:26 13C2-PFOA FC3G 015S016 100- 7.09 4 2.60e5 t \ 1067822 %- . \ iy 0- -- ----------:-----i---------. mmin 7.00 8.00 13C4-PFOA FC3G 015S016 100- 7,09 JT9.03e4 fjft367569 %- /0u ....i...............V...........i.......1 7.00 8.00 13C4-PFOS-1 FC3G 015S016 100- 8.35 1.37e4 51452 o: rrrrr min 13C4-PFOS-2 FC3G 015S016 1~| 8.35 f 1.08e4 1 41238 %" 1 n 0" 1802-PFHxS-1 FC3G 015S016 100-] 7.33 1.21e4"jk .. 47826 J | %" 1802-PFHxS-2 FC3G 015S016 1 00- 7.33 9.22e3 A 36260 I \ %- ttttt min / '... O-' . i . min 0-* . . 1 13C4-PFBA FC3G 015S016 100- 5.19 |~3.54e4 { 158181 %-_ [; 4.03 !: !. \ 0^ ; ...V... -----------------------------t-t- mmmin 5.00 6.00 7.00 13C2-PFHxA FC3G 015S016 100- 6.34 7.15e4 351755 %J !, \ Uo-----------------------------------------------:-- '-- mmmin 5.00 6.00 7.00 13C5-PFNA FC3G 015S016 100 7.49 8.04e4 f\ 325964 j \ uo ---------:-- J` i...................... - i - mmiin 7.00 8.00 1Joo , ooi 13C2-PFDA FC3G 015S016 7.99 i ` 9.19e4 4 321618 u0 --\( -------------------------i--- min 8.00 10.00 12.00 13C2-PFDoA FC3G 015S016 9.18 r 1.41e5 626698 %Z i \ -, u - ---------- - - --- -- -- 8.00 10.00 12.00 13C2-PFOUEA FC3G_015S016 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 277 of 307 www.axysanalytical.com Quantify Sam ple Report M assLyn x 4.1 SCN 627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: FC-LIN-42420W500-D,, Name: FC3G_015S016 Description: 1,,15uL Date: 14-Jan-2013 Tim e: 23:39:26 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-1 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.21 26 262.98 > 218.... 5.97 26 312.97> 268.98 6.34 26 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 27 462.96 >418.... 7.49 28 512,96 >468.... 7.99 28 562.96 > 518.... 8.58 29 612.95 > 568.... 9.18 23 298,94> 79.96 6.47 24 298.94 > 98.95 6.47 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498.93> 79.96 8.35 22 498,93> 98.96 8.35 21 498 > 78 9.96 20 414.97 > 369.... 7.09 416.97 >371.9 7.09 30 503 > 79.96 8.35 30 503 > 98.96 8.35 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.19 30 314.97>269.98 6.34 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.18 458.98 >39.3.98 7.38 Area 60827 77279 141553 139300 151530 36455 142621 180194 192089 223579 42072 20795 33160 19453 10442 32824 22329 23260 259773 90287 13657 10809 12097 9219 35388 71487 80408 91873 140527 30005 IS Area 35388 71487 71487 71487 259773 259773 80408 91873 91873 140527 12097 9219 12097 9219 12097 13657 10809 13657 90287 30005 30005 30005 30005 30005 30005 30005 30005 30005 ng/L 19.340 18.539 20.716 20.106 20.575 20.503 21.652 21.541 21.120 21.812 44.396 38.082 38.704 37.814 40.291 40.254 38.778 18.625 36.417 13.419 18,799 18.725 20.146 20.730 12.007 12.263 12.257 11.132 10.875 10.902 LOD 0.0166 0.3440 0.1133 0.0504 0.1051 0.1255 0.2935 0.0186 0.0041 0.0397 0.0379 0.1825 %Rec 96.7 92.7 103.6 100.5 102.9 102.5 108.3 107.7 105.6 109.1 111.0 95.2 96.8 94.5 100.7 100.6 96.9 93.1 101.2 111.8 104.4 104.0 111.9 115.2 100.1 102.2 102.1 92.8 90.6 109.0 S/N 718 900 1897 2255 0 0 0 614 1936 1914 3718 2023 0 0 178 352 589 807 0 798 499 421 0 0 135 2403 8945 727 946 179 Noise 357 406 359 252 0 0 0 1060 453 492 53 45 0 0 219 257 125 98 0 460 103 98 0 0 1169 146 36 442 662 679 Height 256363 365329 681325 567485 605973 148035 575991 651089 877365 940818 197084 91030 127638 74740 38961 90496 73451 79478 1067822 367569 51452 41238 47826 36260 158181 351755 325964 321618 626698 121616 IS Height 158181 351755 Sample size 1.0000 1.0000 351755 1.0000 351755 1.0000 1067822 1.0000 1067822 1.0000 325964 321618 1.0000 1.0000 321618 626698 47826 1.0000 1.0000 1.0000 36260 47826 1.0000 1.0000 36260 47826 1.0000 1.0000 51452 41238 1.0000 1.0000 51452 1.0000 367569 1.0000 121616 1.0000 1.0000 121616 121616 121616 121616 121616 1.0000 1.0000 1.0000 1.0000 1.0000 121616 121616 121616 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1,0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb PV by LEH 15-Jan-13, SV byAP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 278 of 307 www.axysanalytical.com Quantify Sample Report MassLynx4.1 SCN627 Dataset: G :\M a s s ly n x \ln s t_ G \P F C .P R O \F C \F C 3 G _ 0 1 5 -B .q ld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard T im e Printed; W ednesday, January 16, 2013 11:54:43 Pacific Standard Tim e ID: FC-LIN-42420W 500-D,, Name: FC3GJJ15S036 Description: 1,,15uL Date: 15^Jan-2013 Time: 05:18:50 PFBA FC3G_015S036 100- 5.23 f 6.23e4 I 270678 %- PFPeA FC3G_015S036 100 5.95 7 .4 3 e4 l 342135 %- 1 5.00 6.00 7.00 PFNA FC3G 015S036 5.00 6.00 7.00 PFDA FC3G_015S036 in n 7-99 100 1 f 1.67e5 | 610139 %-' * PFHxS-1 FC3G 015S036 8.00 10.00 12.00 PFHxS-2 FC3G_015S036 100- 7.33 1.84e4 A . 71183 ; \ %- 7.00 8.00 PFHxA FC3G_015S036 100- 6.36 1.37e5~| 636877 'i % 5.00 6.00 7.00 PFUnA FC3G_015S036 100- _ 8.52 1.81e5 804510 %- A 8.00 10.00 12.00 PFHxS-3 FC3G_015S036 100n- 7.33 1.04e4 A : 39987 1 %- ; 7.00 8.00 PFHpA FC3G_015S036 100- 6.73 1.38e5 h 571581 H %- 5.00 6.00 7.00 PFDoA FC3GJD15S036 100: 9.12 2.21 e5 965190 %- 8.00 10.00 12.00 PFOS-1 FC3G 015S036 PFOA-1 FC3G_015S036 100- _ 7.14 r. 1.39e5 ? ;5 46704 O-J-p-j 7.00 8.00 PFBS-1 FC3G__015S036 100- 6.47 3.76e4 169036 n %- 5.00 6.00 7.00 PFOS-2 FC3G__015S036 mn 8-29 100-1 [2 .3 1 6 4 I 81004 %-4 1 8.00 10.00 12.00 PFOA-2 FC3G_015S036 100- 7.14 a 3.37e4 M 134999 %- 7.00 PFBS-2 FC3GJD15S036 100- 6.49 1.95e4 85942 %- 5.00 6.00 7.00 PFOSA FC3G_015S036 9.96 100n 2.2464" ], 76289 ' %- 8.00 10.00 12.00 PV by LEH 15~Jan~13, SV byAP 16-Jan~13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLKConcn. & LOD units = ng/L. Page 279 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst___G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: FC -U N-42420W 500-D,, Name: FC3GJJ15S036 Description: 1,,15uL Date: 15-Jan-2013 Time: 05:18:50 13C2-PFOA FC3G 015S036 100- 7.14 IT 2.46e5 P 992812 %: o7.00 n min 8.00 ooi 1 13C 4 -P F O A FC3G 015S036 7.09 f 8.69e4 H 352889 %: 07.00 . -r-r min 8.00 13C4-PFOS-1 FC3G 015S036 100- 8.29 r~1.33e4 50333 %- . o - .; 8.00 10.00 12.00 13C4-PFBA FC3G 015S036 13C2-PFHXA FC3GJD15S036 100 6.36 7.17e4 332503 %~ l ;\ -- min 5.00 6.00 7.00 13C5-PFNA FC3G_015S036 100n 7.49 8.16e4 h 322312 : \ %- 7.00 8.00 13C4-PFOS-2 FC3G 015S036 100-, 8.29 r 1-05e4 : 1 40542 %- 8.00 10.00 12.00 13C2-PFDA FC3G_015S036 7 99 1001 resiL li 335265 I a&i8.00 10.00 12.00 1802-PFHXS-1 FC3G 015S036 100- 7.33 1.14e4~l 45094 / 7.00 .. 8.00 13C2-PFDOA FC3GJ315S036 loo- f 19.351e25 I 604215 8.00 10.00 12.00 1802-PFHxS-2 FG3G 015S036 100- 7.33 8.5 7 e3 ' l 35796 % 0- j 7.00 8.00 13C2-PFOUEA FC3G 015S036 PV by LEH 15-Jan-13, SV by AP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 280 of 307 www.axysanalytical.com Quantify Sample Report M assLynx 4,1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_015-B.qld Last Altered: Tuesday, January 15, 2013 14:23:27 Pacific Standard Time Printed: Wednesday, January 16, 2013 11:54:43 Pacific Standard Time ID: FC-LIN-42420W 500-D,, Name: FC3GJJ15S036 Description: 1,,15uL Date: 15-Jan-2013 Tim e: 05:18:50 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C4-PFOA 21 13C4-PFOS-1 22 13C4-PFOS-2 23 1802-PFHxS-l 24 1802-PFHxS-2 25 13C4-PFBA 26 13C2-PFHxA 27 13C5-PFNA 28 13C2-PFDA 29 13C2-PFDoA 30 13C2-PFOUEA IS# Trace RT 25 212.99>168.98 5.23 26 262.98 >218.... 5.95 26 312.97> 268.98 6.36 26 362.97> 318.98 6,73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 27 462.96 >418.... 7.49 28 512.96 >468.... 7.99 28 562.96 >518.... 8.52 29 612.95 > 568.... 9.12 23 298.94> 79.96 6.47 24 298.94 > 98.95 6.49 23 398.84 > 79.96 7.33 24 398.84> 98.96 7.33 23 398.84> 118.70 7.33 21 498,93> 79.96 8.29 22 498.93> 98.96 8.29 21 498 > 78 9.96 20 414.97 >369.,.. 7.14 416.97 > 371.9 7.09 30 503 > 79,96 8.29 30 503 > 98.96 8.29 30 402.9>84 7.33 30 402.9>103 7.33 30 216.99>171.98 5.21 30 314.97>269.98 6.36 30 467.96>422.97 7.49 30 514.96 >469.96 7.99 30 614.95 > 569.... 9.12 458.98 >393.98 7.43 Area 62286 74317 136909 138060 138521 33685 140347 167398 181101 220724 37596 19490 34126 18425 10440 33084 23090 22377 245538 86950 13292 10504 11395 8567 34986 71704 81559 89071 135341 30547 IS Area 34986 71704 71704 71704 245538 245538 81559 89071 89071 135341 11395 8567 11395 8567 11395 13292 10504 13292 86950 30547 30547 30547 30547 30547 30547 30547 30547 30547 ng/L 20.039 17.773 19.966 19.865 19.890 20.040 21.002 20.617 20.528 22.370 42.087 38.412 42.337 38.544 42.778 41.705 41.276 18.413 35.742 12.923 17.971 17.874 18.641 18.922 11.660 12.082 12.211 10.601 10.288 11.099 LOD 0.0058 0.0116 0.0791 0.0816 0.0247 0.0219 0.0782 0.1182 0.3104 1.1940 0.0248 0.0031 0.0515 0.0485 %Rec 100.2 88.9 99.8 99.3 99.5 100.2 105.0 103.1 102.6 111.9 105.2 96.0 105.8 96.4 106.9 104.3 103.2 92.1 99.3 107.7 99.8 99.3 103.6 105.1 97.2 100.7 101.8 88.3 85.7 111.0 S/N 1273 1090 3287 2428 0 0 0 962 1638 2358 7185 2455 8257 991 2241 994 346 1591 4321 1774 670 449 0 196 31 1739 11832 597 729 0 Noise 213 314 194 235 0 0 0 634 491 409 24 35 16 72 18 99 234 48 230 199 75 90 0 183 4654 191 27 562 829 0 Height 270678 342135 636877 571581 546704 134999 571240 610139 804510 965190 169036 85942 131746 71183 39987 98019 81004 76289 992812 352889 50333 40542 45094 35796 144745 332503 322312 335265 604215 119051 IS Height Sample size 144745 1.0000 332503 332503 1.0000 1.0000 332503 1.0000 992812 1.0000 992812 1.0000 322312 1.0000 335265 1.0000 335265 1.0000 604215 1.0000 45094 1.0000 35796 1.0000 45094 1.0000 35796 1.0000 45094 1.0000 50333 1.0000 40542 1.0000 50333 1.0000 352889 1.0000 1.0000 119051 1.0000 119051 1.0000 119051 1.0000 119051 1.0000 119051 1.0000 119051 1.0000 119051 1.0000 119051 1.0000 119051 1.0000 1.0000 Factor2 Detection... 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1,0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb . 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1,0 bb 1.0 bb 1.0 bb PV by LEH 15-Jan-13, SV byAP 16-Jan-13. NOTE: OPR Conen. & LOD units = ng/mL, Sample and Lab BLK Conen. & LOD units = ng/L. Page 281 of 307 www.axysanalytical.com Matrix Codes for Table 1a N P W = N o n -P o ta b le W a te r D rW = D rin k in g W a te r S= S o lid T= T is s u e AXYS Analytical Services Ltd. Table 1a NELAP Accreditation Held by AXYS Analytical Services Ltd. for Chlorinated Dioxins/Furans, Chlorinated Pesticides, PCBs and PAHs Accreditation Method Codes and Explanation for Table 1a Code Accreditation Certificate Applicable AXYS Method and Description No. Method Reference 1 EPA 1613B MLA-017, performance based implementation of EPA1613B (GC/HRMS) 2 EPA 8290 MLA-017, performance based implementation of EPA 8290 (Gc /HRMS) 3 AXYS MLA-017 MLA-017, performance based implementation of EPA 1613B, 8290 (GC/HRMS) 4 EPA 608 MLA-007, performance based implementation of EPA 608 (GC/ECD) 5 EPA 8270C or 8270D MLA-007, performance based modification of 8270C/D (GC/LRMS) 6 E P A 8081A or8081B MLA-007, performance based implementation of EPA 8 0 8 1 A/B (GC/ECD) 7 EPA 1668A MLA-010, performance based implementation of EPA 1668A (GC/HRMS) 8 SM 6630B MLA-007, performance based implementation of SM 18-20 6630B (GC/ECD) 9 EPA 1625B MLA-021, performance based modification of EPA 1625B (GC/LRMS) 11 EPA 625 MLA-007, performance based modification of EPA 625 (g C/LRMS) 20 EPA 8270C or 8270D MLA-021, performance based modification of EPA 8270C/D (GC/LRMS) 21 EPA 1625B MLA-007, performance based modification of EPA 1625B (GC/LRMS) ACC-101 Rev 08, 08-Nov-2012 Pa9e, o J l < * Page 282 of 307 www.axysanalytical.com TABLE 1a PCDD/F - Polychlorinated Dioxins and Furans Dioxins Dioxins and Dibenzofurans 1,2,3,4,6,7,8-HpCDD 1,2,3,4,6,7,8-HpCDF 1,2,3,4,7,8,9-HpCDF 1,2,3,4,7,8-HxCDD 1,2,3,4,7,8-HxCDF 1,2,3,6,7,8-HxCDD 1,2,3,6,7,8-HxCDF 1,2,3,7,8,9-HxCDD 1,2,3,7,8,9-HxCDF 1,2,3,7,8-PeCDD 1,2,3,7,8-PeCDF 2,3,4,6,7,8-HxCDF 2,3,4,7,8-PeCDF 2,3,7,8-TCDD 2,3,7,8-TCDF OCDD OCDF Total TCDD Total TCDF Total PeCDD Total PeCDF Total HxCDD Total HxCDF Total HpCDD Total HpCDF PCBs - Polychlorinated biphenyls PCB 1 2-Chlorobiphenyl PCB 3 4-Chlorobiphenyl PCB 4 2,2'-Dichlorobiphenyl PCB 5 2,3-Dichlorobiphenyl PCB 15 4,4'-Dichlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. New York State D ep artm en t of Health C a lifo rn ia D ep artm en t of Public Health L a b ID 11674 N E LA P P rim a ry L a b ID 01138C A N E LA P S e co n d a ry NP S NP S WW State of Florida D ep artm en t of Health L a b ID E 8 7 1 0 0 7 N E L A P P rim a ry Dr. NP WW S T 1 2 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 1 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 State of New Jersey Departm ent of E n v iro n m e n ta l Protection L a b ID C A N A 0 0 5 N E L A P S e co n d a ry Dr. NP WW S T 122 122 122 122 122 122 122 122 122 122 122 122 122 122 122 122 122 22 22 22 22 22 22 22 22 Virginia Division of Consolidated Laboratory Services L a b ID 4 6 0 2 2 4 N E L A P S e co n d a ry NP S W T 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 22 22 22 22 22 22 22 22 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 Page 2 o f mJ? Page 283 of 307 www.axysanalytical.com TABLE 1a PCB 18 PCB 19 PCB 31 PCB 37 PCB 44 PCB 52 PCB 54 PCB 66 PCB 77 PCB 81 PCB 87 PCB 101 PCB 104 PCB 105 PCB 109 PCB 114 PCB 118 PCB 123 PCB 124 PCB 126 PCB 138 PCB 141 PCB 151 PCB 153 PCB 155 PCB 156 PCB 157 PCB 167 PCB 169 PCB 170 PCB 180 PCB 183 PCB 187 PCB 188 PCB 189 PCB 202 2,2',5-T richlorobiphenyl 2,2',6-T richlorobiphenyl 2 ,4 ',5 -T ric h lo ro b ip h e n y l 3 ,4 ,4 '-T ric h lo ro b ip h e n y l 2 ,2 ',3 ,5 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',5 ,5 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',6 ,6 '-T e tra c h lo ro b ip h e n y l 2 ,3 ',4 ,4 '-T e tra c h lo ro b ip h e n y l 3 ,3 ',4 ,4 '-T e tra c h lo ro b ip h e n y l 3 ,4 ,4 ',5 -T e tra c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',4 ,5 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',4 ,6 ,6 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ,4 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ,6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ,4 ',5 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ',5 ,5 '-P e n ta c h lo ro b ip h e n y l 3 ,3 ',4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2,2',3,4,4',5'-H exachlorobiphenyl 2,2',3,4,5,5'-H exachlorobiphenyl 2,2',3,5,5',6-H exachlorobiphenyl 2,2',4,4',5,5'-H exachlorobiphenyl 2,2',4,4',6,6'-H exachlorobiphenyl 2,3,3',4,4',5-H exachlorobiphenyl 2,3,3',4,4',5'-H exachlorobiphenyl 2,3',4,4',5,5'-H exachlorobiphenyl 3,3',4,4',5,5'-H exachlorobiphenyl 2,2',3,3',4,4',5-H eptachlorobiphenyl 2,2',3,4,4',5,5'-H eptachlorobiphenyl 2,2',3,4,4',5',6-H eptachlorobiphenyl 2,2',3,4',5,5',6-H eptachlorobiphenyl 2,2',3,4',5,6,6'-H eptachlorobiphenyl 2,3,3',4,4',5,5'-H eptachlorobiphenyl 2,2',3,3',5,5',6,6'-O ctachlorobiphenyl A C C -10 1 R e v 08, 08-N o v-2012 AXYS Analytical Services Ltd. New York State D ep artm en t of Health C a lifo rn ia D ep artm en t of Public Health L a b ID 11674 N E LA P P rim a ry L a b ID 01138C A N E LA P S e co n d a ry NP S NP S WW 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 7 77 77 77 77 77 77 77 77 77 77 State of Florida D ep artm en t of Health L a b ID E 8 7 1 0 0 7 N E L A P P rim a ry Dr. NP WW S T State of New Jersey Departm ent of E n v iro n m e n ta l Protection L a b ID C A N A 0 0 5 N E L A P S e co n d a ry Dr. NP WW 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 S 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 T Virginia Division of Consolidated Laboratory Services L a b ID 4 6 0 2 2 4 N E L A P S e co n d a ry NP S W 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 7 77 77 77 77 77 77 77 77 77 77 T Page 3 o f-J * Page 284 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 1a PCB 205 PCB 206 PCB 208 PCB 209 Aroclor 1260 Aroclor 1254 Aroclor 1221 Aroclor 1232 Aroclor 1248 Aroclor 1016 Aroclor 1242 2,3,3',4,4',5,5',6-Octachlorobiphenyl 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 2,2',3,3',4,5,5',6,6'-Nonachlorobiphenyl Decachlorobiphenyl Pesticides 4,4'-DDD 4,4'-DDE 4,4'-DDT Aldrin Alpha-HCH Beta-HCH cis-Chlordane (alpha-Chlordane) Chlordane, technical Delta-HCH Dieldrin Endosulphan I Endosulphan II Endosulphan sulphate Endrin Endrin aldehyde trans-Chlordane (gamma-Chlordane) Gamma-HCH (Lindane) Heptachlor Heptachlor epoxide Hexachlorobenzene Methoxychlor Mi rex New York State D ep artm en t of Health Lab ID 11674 NELAP Primary NP S W 7 7 7 7 7, 11 7, 11 7, 11 7, 11 7, 11 7, 11 7, 11 7 7 7 7 5, 7 5, 7 5, 7 5, 7 5, 7 5, 7 5, 7 C a lifo rn ia D ep artm en t of Public Health Lab ID 01138CA NELAP Secondary NP S W 11 5 11 5 11 5 11 5 11 5 11 5 11 5 State of Florida D ep artm en t of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 55 5, 11 5 11 5 11 5 11 5 4646 4646 4646 4646 4646 4646 55 11 5 11 5 11 5 11 5 4646 21 5 9 5 4, 8 6 8 6 5 ACC-101 Rev 08, 08-Nov-2012 State of New Jersey Departm ent of E n v iro n m e n ta l Protection Lab ID CANA005 NELAP Secondary Dr. NP WW 7 7 7 7 S 7 7 7 7 T Virginia Division of Consolidated Laboratory Services Lab ID 460224 NELAP Secondary NP S W 77 77 77 77 11 5 11 5 11 5 11 5 11 5 11 5 11 5 T 11 11 11 11 11 11 5, 11 11 4 4 4 4 4 4 11 11 4 4 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 5 5 6 5 6 Page 4 o f Page 285 of 307 www.axysanalytical.com TABLE 1a PAH Anthracene Pyrene Benzo[ghilperylene Indeno[1,2,3-cdlpyrene Benzo[blfluoranthene Fluoranthene Benzo[k]fluoranthene Acenaphthylene Chrysene Benzo[a]pyrene Dibenz[ahlanthracene Benz[alanthracene Acenaphthene Phenanthrene Fluorene Naphthalene AXYS Analytical Services Ltd. New York State D ep artm en t of Health Lab ID 11674 NELAP Primary NP S W C a lifo rn ia D ep artm en t of Public Health Lab ID 01138CA NELAP Secondary NP S W State of Florida D ep artm en t of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 9 20 9 20 9 20 9 20 9 20 9 20 9 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 State of New Jersey Departm ent of E n v iro n m e n ta l Protection Lab ID CANA005 NELAP Secondary Dr. NP WW S T Virginia Division of Consolidated Laboratory Services Lab ID 460224 NELAP Secondary NP S W 9 20 9 20 9 20 9 20 9 20 9 20 9 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 T ACC-101 Rev 08, 08-Nov-2012 Pa9e5oJ l < * Page 286 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. Table 1b NELAP Accreditation Held by AXYS Analytical Services Ltd. for Perfluorinated Organic Compounds Matrix Codes for Table 1b N P W = N o n -P o ta b le W a te r D rW = D rin k in g W a te r S = Solid T = Tissue Accreditation Method Codes and Explanation for Table 1b Code Accreditation Certificate Applicable AXYS Method and Description No. Method Reference 12 AXYS MLA-041 13 AXYS MLA-043 14 AXYS MLA-060 MLA-041, laboratory performance based method (LC/MS-MS) MLA-043, laboratory performance based method (LC/MS-MS) MLA-060, laboratory performance based method (LC/MS-MS) TA BLE 1b State of Florida D ep artm en t of Health L a b ID E 8 7 1 0 0 7 N E L A P P rim a ry Dr. NP S WW T Minnesota Department of Health L a b ID 2 3 2 -9 9 9 -4 3 0 N E L A P P r i m a r y! Dr. NP S T WW State of New Jersey Departm ent of E n v iro n m e n ta l Protection L a b ID C A N A 0 0 5 N E L A P S e co n d a ry Dr. NP S T WW PFC - Perfluorinated Organic Compounds Perfluorobutanoate (PFBA) Note Perfluoropentanoate (PFPeA) Note Perfluorohexanoate (PFHxA) Note Perfluoroheptanoate (PFHpA) Note Perfluorooctanoate (PFOA) Note Perfluorononanoate (PFNA) Note Perfluorodecanoate (PFDA) Note Perfluoroundecanoate (PFUnA) Note Perfluorododecanoate (PFDoA) Note Perfluorobutanesulfonate (PFBS) Perfluorohexanesulfonate (PFHxS) Perfluorooctanesulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 Note: Accreditations by Minnesota Department of Health and New Jersey Department of Environmental Protection are against the corresponding acid form of the anion shown. ACC-101 Rev 08, 08-Nov-2012 Pa9e6oJ l & Page 287 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. Table 2: Canadian and US State Specific Accreditation Held by AXYS Analytical Services Ltd. Matrix Codes for Table 2 NP W = D r. W = W= S= T= Ser = U= N o n -P o ta b le W a te r D rin k in g W a te r Aqueous S o lid T is s u e S e ru m /P la s m a U rin e Accreditation Method Codes and Explanation for Table 2 Code Accreditation Certificate No. Method Reference Applicable AXYS Method and Description 1 EPA 1613 3 AXYS MLA-017 7 EPA 1668A 10 AXYS MLA-007 12 AXYS MLA-041 13 AXYS MLA-043 14 AXYS MLA-060 15 AXYS MLA-010 16 AXYS MLA-028 17 AXYS MLA-033 18 AXYS MLA-021 19 AXYS MLA-075 22 AXYS MLA-901 23 AXYS MLA-042 24 AXYS MLA-070 25 AXYS MLA-079 26 AXYS MLA-059 27 AXYS MLA-064 28 EPA 1668C 29 AXYS MLA-021 MLA-017 Performance based implementation of EPA 1613B (GC/HRMS) MLA-017 Performance based implementation of EPA 1613B (gC/HRMs) MLA-010 Performance based implementation of EPA 1668A (gC/HRMs) MLA-007, Performance based modification of EPA 8270C/D, 8081A/B (GC/LRMS and GC/ECD) MLA-041 Laboratory performance based method (LC/MS-MS) MLA-043 Laboratory performance based method (LC/MS-MS) MLA-060 Laboratory performance based method (LC/MS-MS) MLA-010 Performance based implementation of EPA 1668A (GC/HRMS) MLA-028 Laboratory performance based method (GC/HRMS) MLA-033 Performance based implementation of EPA 1614 (Gc/HRMS) MLA-021 Performance based modification of EPA 8270C/D (GC/LRMS) MLA-075 Performance based implementation of EPA 1694 (LC/MS-MS) MLA-901 Laboratory performance based method (GC/LRmS) MLA-042 Laboratory performance based method (LC/MS-MS) MLA-070 Laboratory performance based method (LC/MS-MS) MLA-079 Laboratory performance based method (LC/MS-MS) MLA-059 Laboratory performance based method (LC/MS-MS) MLA-064 Laboratory performance based method (LC/MS-MS) MLA-010 Performance based implementation of EPA 1668C (GC/HRMS) MLA-021 Performance based modification of EPA 1625 (GC/LRMS) ACC-101 Rev 08, 08-Nov-2012 Pa9e 7 o J l < * Page 288 of 307 www.axysanalytical.com TABLE 2 PCDD/F - Polychlorinated Dioxins and Furans 1,2,3,4,6,7,8-HpCDD 1,2,3,4,6,7,8-HpCDF 1,2,3,4,7,8,9-HpCDF 1,2,3,4,7,8-HxCDD 1,2,3,4,7,8-HxCDF 1,2,3,6,7,8-HxCDD 1,2,3,6,7,8-HxCDF 1,2,3,7,8,9-HxCDD 1,2,3,7,8,9-HxCDF 1,2,3,7,8-PeCDD 1,2,3,7,8-PeCDF 2,3,4,6,7,8-HxCDF 2,3,4,7,8-PeCDF 2,3,7,8-TCDD 2,3,7,8-TCDF OCDD OCDF PCBs - Polychlorinated biphenyls PCB 1 2-Chlorobiphenyl PCB 2 3-Chlorobiphenyl PCB 3 4-Chlorobiphenyl PCB 4 2,2'-Dichlorobiphenyl PCB 5 2,3-Dichlorobiphenyl PCB 6 2,3'-Dichlorobiphenyl PCB 7 2,4-Dichlorobiphenyl PCB 8 2,4'-Dichlorobiphenyl PCB 8/5 PCB 9 2,5-Dichlorobiphenyl PCB 10 2,6-Dichlorobiphenyl PCB 11 3,3'-Dichlorobiphenyl PCB 12 3,4-Dichlorobiphenyl PCB 13 3,4'-Dichlorobiphenyl PCB 14 3,5-Dichlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology A c c re d ita tio n n o .: A 2 6 3 7 L a b . ID : C 4 0 4 W S Pulp T Ser U NP S W 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 10 10 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 Pa9eSoJ l & Page 289 of 307 www.axysanalytical.com TABLE 2 PCB 15 4,4'-Dichlorobiphenyl PCB 16 2,2',3-T richlorobiphenyl PCB 16/32 PCB 17 2,2',4-T richlorobiphenyl PCB 18 2,2',5-Trichlorobiphenyl PCB 19 2,2',6-T richlorobiphenyl PCB 20 2,3,3'-Trichlorobiphenyl PCB 21 2,3,4-T richlorobiphenyl PCB 22 2,3,4'-Trichlorobiphenyl PCB 23 2,3,5-T richlorobiphenyl PCB 24 2,3,6-T richlorobiphenyl PCB 24/27 PCB 25 2,3',4-T richlorobiphenyl PCB 26 2,3',5-Trichlorobiphenyl PCB 27 2,3',6-Trichlorobiphenyl PCB 28 2,4,4'-Trichlorobiphenyl PCB 29 2,4,5-T richlorobiphenyl PCB 30 2,4,6-T richlorobiphenyl PCB 31 2,4',5-Trichlorobiphenyl PCB 32 2,4',6-Trichlorobiphenyl PCB 33 2,3',4'-Trichlorobiphenyl PCB 33/20/21 PCB 34 2,3',5'-Trichlorobiphenyl PCB 35 3,3',4-Trichlorobiphenyl PCB 36 3,3',5-Trichlorobiphenyl PCB 37 3,4,4'-Trichlorobiphenyl PCB 38 3,4,5-T richlorobiphenyl PCB 39 3,4',5-Trichlorobiphenyl PCB 40 2,2',3,3'-Tetrachlorobiphenyl PCB 41 2,2',3,4-Tetrachlorobiphenyl PCB 41/71/64/68 PCB 42 2,2',3,4'-Tetrachlorobiphenyl PCB 42/59 PCB 43 2,2',3,5-Tetrachlorobiphenyl PCB 44 2,2',3,5'-Tetrachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) A c c re d ita tio n n o .: A 2 6 3 7 W S Pulp T Ser 10, 15 15 10 10, 15 10, 15 10, 15 15 15 10, 15 15 15 10 10, 15 10, 15 15 10, 15 15 15 10, 15 15 15 18 15 15 15 15 15 15 10, 15 15 10 15 10 15 10, 15 10, 15 15 10 10, 15 10, 15 10, 15 15 15 10, 15 15 15 10 10, 15 10, 15 15 10, 15 15 15 10, 15 15 15 10 15 15 15 15 15 15 10, 15 15 10 15 10 15 10, 15 10, 15 15 10 10, 15 10, 15 10, 15 15 15 10, 15 15 15 10 10, 15 10, 15 15 10, 15 15 15 10, 15 15 15 10 15 15 15 15 15 15 10, 15 15 10 15 10 15 10, 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 W ashing to n State D epartm ent of Ecology L a b . ID : C 4 0 4 U NP S W 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 Pa9e9oJ l < * Page 290 of 307 www.axysanalytical.com TABLE 2 PCB 45 PCB 46 PCB 47 PCB 47/48/75 PCB 48 PCB 49 PCB 49/43 PCB 50 PCB 51 PCB 52 PCB 52/73 PCB 53 PCB 54 PCB 55 PCB 56 PCB 56/60 PCB 57 PCB 58 PCB 59 PCB 60 PCB 61 PCB 62 PCB 63 PCB 64 PCB 65 PCB 66 PCB 66/80 PCB 67 PCB 68 PCB 69 PCB 70 PCB 70/76 PCB 71 PCB 72 PCB 73 2,2',3,6-Tetrachlorobiphenyl 2,2',3,6'-Tetrachlorobiphenyl 2,2',4,4'-Tetrachlorobiphenyl 2,2',4,5-Tetrachlorobiphenyl 2,2',4,5'-Tetrachlorobiphenyl 2,2',4,6-Tetrachlorobiphenyl 2,2',4,6'-Tetrachlorobiphenyl 2,2',5,5'-Tetrachlorobiphenyl 2,2',5,6'-Tetrachlorobiphenyl 2,2',6,6'-Tetrachlorobiphenyl 2,3,3',4-Tetrachlorobiphenyl 2,3,3',4'-Tetrachlorobiphenyl 2,3,3',5-Tetrachlorobiphenyl 2,3,3',5'-Tetrachlorobiphenyl 2,3,3',6-Tetrachlorobiphenyl 2,3,4,4-Tetrachlorobiphenyl 2,3,4,5-Tetrachlorobiphenyl 2,3,4,6-Tetrachlorobiphenyl 2,3,4',5-Tetrachlorobiphenyl 2,3,4',6-Tetrachlorobiphenyl 2,3,5,6-Tetrachlorobiphenyl 2,3',4,4'-Tetrachlorobiphenyl 2,3',4,5-Tetrachlorobiphenyl 2,3',4,5'-Tetrachlorobiphenyl 2,3',4,6-Tetrachlorobiphenyl 2,3',4',5-Tetrachlorobiphenyl 2,3',4',6-Tetrachlorobiphenyl 2,3',5,5'-Tetrachlorobiphenyl 2,3',5',6-Tetrachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) A c c re d ita tio n n o .: A 2 6 3 7 W S Pulp T Ser 10, 15 10, 15 15 10 15 15 10 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 10 15 15 15 10, 15 10, 15 15 10 15 15 10 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 10 15 15 15 10, 15 10, 15 15 10 15 15 10 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 W ashing to n State D epartm ent of Ecology L a b . ID : C 4 0 4 U NP S W 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 Page 291 of 307 www.axysanalytical.com TABLE 2 PCB 74 2,4,4',5-Tetrachlorobiphenyl PCB 74/61 PCB 75 2,4,4',6-Tetrachlorobiphenyl PCB 76 2,3',4',5'-Tetrachlorobiphenyl PCB 77 3,3',4,4'-Tetrachlorobiphenyl PCB 78 3,3',4,5-Tetrachlorobiphenyl PCB 79 3,3',4,5'-Tetrachlorobiphenyl PCB 80 3,3',5,5'-Tetrachlorobiphenyl PCB 81 3,4,4',5-Tetrachlorobiphenyl PCB 82 2,2',3,3',4-Pentachlorobiphenyl PCB 83 2,2',3,3',5-Pentachlorobiphenyl PCB 83/108 PCB 84 2,2',3,3',6-Pentachlorobiphenyl PCB 85 2,2',3,4,4'-Pentachlorobiphenyl PCB 85/120 PCB 86 2,2',3,4,5-Pentachlorobiphenyl PCB 87 2,2',3,4,5'-Pentachlorobiphenyl PCB 87/115/116 PCB 88 2,2',3,4,6-Pentachlorobiphenyl PCB 89 2,2',3,4,6'-Pentachlorobiphenyl PCB 90 2,2',3,4',5-Pentachlorobiphenyl PCB 91 2,2',3,4',6-Pentachlorobiphenyl PCB 92 2,2',3,5,5'-Pentachlorobiphenyl PCB 93 2,2',3,5,6-Pentachlorobiphenyl PCB 94 2,2',3,5,6'-Pentachlorobiphenyl PCB 95 2,2',3,5',6-Pentachlorobiphenyl PCB 95/93 PCB 96 2,2',3,6,6'-Pentachlorobiphenyl PCB 97 2,2',3,4',5'-Pentachlorobiphenyl PCB 97/86 PCB 98 2,2',3,4',6'-Pentachlorobiphenyl PCB 99 2,2',4,4',5-Pentachlorobiphenyl PCB 100 2,2',4,4',6-Pentachlorobiphenyl PCB 101 2,2',4,5,5'-Pentachlorobiphenyl PCB 101/90/89 ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology A c c re d ita tio n n o .: A 2 6 3 7 L a b . ID : C 4 0 4 W S Pulp T Ser U NP S W 15 15 15 15, 22 28 28 10 10 15 15 10 15 15 28 28 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 15 15 15 15 15 15 15 15 28 28 28 28 15 15 15 15 28 28 15 15 15 10 10, 15 15 15 15 10 10, 15 15 15 15 10 10, 15 15 15 15 15 28 28 28 28 28 28 28 28 15 15 15 15 28 28 10 10 15 15 15 15 10 15 15 15 15 28 28 28 28 10 10 10 15 15 15 15 15 15 15 15 28 28 28 28 15 10, 15 15 15 15 15 15 10, 15 15 15 15 15 15 10, 15 15 15 15 15 15 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 28 28 10 10 15 15 10 15 15 28 28 15 15 15 15 28 28 10 15 10, 15 10 15 10, 15 10 15 10, 15 15 15, 22 28 28 28 28 15 15 15 15 28 28 15 15 10 10 15 15 10 28 28 Page 292 of 307 www.axysanalytical.com TABLE 2 PCB 102 PCB 103 PCB 104 PCB 105 PCB 105/127 PCB 106 PCB 107 PCB 107/109 PCB 108 PCB 109 PCB 110 PCB 111 PCB 112 PCB 113 PCB 114 PCB 115 PCB 116 PCB 117 PCB 118 PCB 118/116 PCB 119 PCB 120 PCB 121 PCB 122 PCB 123 PCB 124 PCB 125 PCB 126 PCB 127 PCB 128 PCB 129 PCB 130 PCB 131 PCB 131/142 PCB 132 2,2',4,5,6'-Pentachlorobiphenyl 2,2',4,5',6-Pentachlorobiphenyl 2,2',4,6,6'-Pentachlorobiphenyl 2,3,3',4,4'-Pentachlorobiphenyl 2,3,3',4,5-Pentachlorobiphenyl 2,3,3',4',5-Pentachlorobiphenyl 2,3,3',4,5'-Pentachlorobiphenyl 2,3,3',4,6-Pentachlorobiphenyl 2,3,3',4',6-Pentachlorobiphenyl 2,3,3',5,5'-Pentachlorobiphenyl 2,3,3',5,6-Pentachlorobiphenyl 2,3,3',5',6-Pentachlorobiphenyl 2,3,4,4',5-Pentachlorobiphenyl 2,3,4,4',6-Pentachlorobiphenyl 2,3,4,5,6-Pentachlorobiphenyl 2,3,4',5,6-Pentachlorobiphenyl 2,3',4,4',5-Pentachlorobiphenyl 2,3',4,4',6-Pentachlorobiphenyl 2,3',4,5,5'-Pentachlorobiphenyl 2,3',4,5',6-Pentachlorobiphenyl 2,3,3',4',5'-Pentachlorobiphenyl 2,3',4,4',5'-Pentachlorobiphenyl 2,3',4',5,5'-Pentachlorobiphenyl 2,3',4',5',6-Pentachlorobiphenyl 3,3',4,4',5-Pentachlorobiphenyl 3,3',4,5,5'-Pentachlorobiphenyl 2,2',3,3',4,4'-Hexachlorobiphenyl 2,2',3,3',4,5-Hexachlorobiphenyl 2,2',3,3',4,5'-Hexachlorobiphenyl 2,2',3,3',4,6-Hexachlorobiphenyl 2,2',3,3',4,6'-Hexachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology A c c re d ita tio n n o .: A 2 6 3 7 L a b . ID : C 4 0 4 W S Pulp T Ser U NP S W 15 15 15 15 28 28 15 15 15 15 15 15 15 15 28 28 28 28 15 15 10 10 15 15 10 28 28 15 15 15 15 15 15 15 15 28 28 28 28 10 10 10 15 15 10, 15 15 15 15 15 10, 15 15 15 15 15 10, 15 15 15 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 15 15 15 15 28 28 10, 15 15 15 10, 15 15 15 10, 15 15 15 15 15 15 28 28 28 28 28 28 15 15 15 15 28 28 15 15 10 10 15 15, 22 10 28 28 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 28 28 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 15 15 15 28 28 28 28 28 28 15 15 15 15 28 28 10 10 15 15 10 15 15 28 28 Page 293 of 307 www.axysanalytical.com TABLE 2 PCB 133 2,2',3,3',5,5'-Hexachlorobiphenyl PCB 134 2,2',3,3',5,6-Hexachlorobiphenyl PCB 134/143 PCB 135 2,2',3,3',5,6'-Hexachlorobiphenyl PCB 136 2,2',3,3',6,6'-Hexachlorobiphenyl PCB 137 2,2',3,4,4',5-Hexachlorobiphenyl PCB 138 2,2',3,4,4',5'-Hexachlorobiphenyl PCB 138/163/164 PCB 139 2,2',3,4,4',6-Hexachlorobiphenyl PCB 140 2,2',3,4,4',6'-Hexachlorobiphenyl PCB 141 2,2',3,4,5,5'-Hexachlorobiphenyl PCB 142 2,2',3,4,5,6-Hexachlorobiphenyl PCB 143 2,2',3,4,5,6'-Hexachlorobiphenyl PCB 144 2,2',3,4,5',6-Hexachlorobiphenyl PCB 144/135 PCB 145 2,2',3,4,6,6'-Hexachlorobiphenyl PCB 146 2,2',3,4',5,5'-Hexachlorobiphenyl PCB 147 2,2',3,4',5,6-Hexachlorobiphenyl PCB 148 2,2',3,4',5,6'-Hexachlorobiphenyl PCB 149 2,2',3,4',5',6-Hexachlorobiphenyl PCB 149/139 PCB 150 2,2',3,4',6,6'-Hexachlorobiphenyl PCB 151 2,2',3,5,5',6-Hexachlorobiphenyl PCB 152 2,2',3,5,6,6'-Hexachlorobiphenyl PCB 153 2,2',4,4',5,5'-Hexachlorobiphenyl PCB 154 2,2',4,4',5,6'-Hexachlorobiphenyl PCB 155 2,2',4,4',6,6'-Hexachlorobiphenyl PCB 156 2,3,3',4,4',5-Hexachlorobiphenyl PCB 157 2,3,3',4,4',5'-Hexachlorobiphenyl PCB 158 2,3,3',4,4',6-Hexachlorobiphenyl PCB 158/160 PCB 159 2,3,3',4,5,5'-Hexachlorobiphenyl PCB 160 2,3,3',4,5,6-Hexachlorobiphenyl PCB 161 2,3,3',4,5',6-Hexachlorobiphenyl PCB 162 2,3,3',4',5,5'-Hexachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology A c c re d ita tio n n o .: A 2 6 3 7 L a b . ID : C 4 0 4 W S Pulp T Ser U NP S W 15 15 15 15 28 28 15 15 10 10 15 15 10 28 28 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 10, 15 10, 15 15 15 10, 15 15 15 15, 22 28 28 28 28 10 10 10 15 15 10, 15 15 15 15 15 10, 15 15 15 15 15 10, 15 15 15 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 15 15 15 15 28 28 10 15 10, 15 10 15 10, 15 10 15 10, 15 15 15, 22 28 28 28 28 15 15 15 15 28 28 15 15 15 15 15 15 15 15 28 28 28 28 10 15 10, 15 15 10, 15 15 10 15 10, 15 15 10, 15 15 10 15 10, 15 15 10, 15 15 15 15 15 15, 22 15 28 28 28 28 28 28 28 28 28 28 15 15 10, 15 10, 15 15 15 10, 15 15, 22 28 28 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 10 10 15 15 15 15 10 15 15 28 28 28 28 15 15 15 15 28 28 15 15 15 15 15 15 15 15 28 28 28 28 Page 294 of 307 www.axysanalytical.com TABLE 2 PCB 163 PCB 164 PCB 165 PCB 166 PCB 167 PCB 168 PCB 169 PCB 170 PCB 170/190 PCB 171 PCB 172 PCB 172/192 PCB 173 PCB 174 PCB 174/181 PCB 175 PCB 176 PCB 177 PCB 178 PCB 179 PCB 180 PCB 181 PCB 182 PCB 183 PCB 184 PCB 185 PCB 186 PCB 187 PCB 187/182 PCB 188 PCB 189 PCB 190 PCB 191 PCB 192 PCB 193 2,3,3',4',5,6-Hexachlorobiphenyl 2,3,3',4',5',6-Hexachlorobiphenyl 2,3,3',5,5',6-Hexachlorobiphenyl 2,3,4,4',5,6-Hexachlorobiphenyl 2,3',4,4',5,5'-Hexachlorobiphenyl 2,3',4,4',5',6-Hexachlorobiphenyl 3,3',4,4',5,5'-Hexachlorobiphenyl 2,2',3,3',4,4',5-Heptachlorobiphenyl 2,2',3,3',4,4',6-Heptachlorobiphenyl 2,2',3,3',4,5,5'-Heptachlorobiphenyl 2,2',3,3',4,5,6-Heptachlorobiphenyl 2,2',3,3',4,5,6'-Heptachlorobiphenyl 2,2',3,3',4,5',6-Heptachlorobiphenyl 2,2',3,3',4,6,6'-Heptachlorobiphenyl 2,2',3,3',4,5',6'-Heptachlorobiphenyl 2,2',3,3',5,5',6-Heptachlorobiphenyl 2,2',3,3',5,6,6'-Heptachlorobiphenyl 2,2',3,4,4',5,5'-Heptachlorobiphenyl 2,2',3,4,4',5,6-Heptachlorobiphenyl 2,2',3,4,4',5,6'-Heptachlorobiphenyl 2,2',3,4,4',5',6-Heptachlorobiphenyl 2,2',3,4,4',6,6'-Heptachlorobiphenyl 2,2',3,4,5,5',6-Heptachlorobiphenyl 2,2',3,4,5,6,6'-Heptachlorobiphenyl 2,2',3,4',5,5',6-Heptachlorobiphenyl 2,2',3,4',5,6,6'-Heptachlorobiphenyl 2,3,3',4,4',5,5'-Heptachlorobiphenyl 2,3,3',4,4',5,6-Heptachlorobiphenyl 2,3,3',4,4',5',6-Heptachlorobiphenyl 2,3,3',4,5,5',6-Heptachlorobiphenyl 2,3,3',4',5,5',6-Heptachlorobiphenyl AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology A c c re d ita tio n n o .: A 2 6 3 7 L a b . ID : C 4 0 4 W S Pulp T Ser U NP S W 15 15 15 15 28 28 15 15 15 15 15 15 15 15 28 28 28 28 15 15 15 15 15 15 15 15 28 28 28 28 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 15 15 15 15, 22 28 28 10 10, 15 15 10 15 10 10, 15 15 10 15 10 10, 15 15 10 15 15 15 15 28 28 28 28 28 28 15 15 15 15 28 28 10 10, 15 10, 15 10 10, 15 10, 15 10 10, 15 10, 15 15 15 28 28 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 15 15 28 28 28 28 10, 15 15 15 10, 15 15 10, 15 10, 15 15 15 10, 15 15 10, 15 10, 15 15 15 10, 15 15 10, 15 15, 22 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 28 28 15 15 15 15 15 15 15 15, 22 28 28 28 28 10 10 10 15 10, 15 15 15 10, 15 15 15 10, 15 15 15 15 15 28 28 28 28 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 ACC-101 Rev 08, 08-Nov-2012 Page 295 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 Canadian Association for Laboratory Accreditation (CALA) A c c re d ita tio n n o .: A 2 6 3 7 W ashing to n State D epartm ent of Ecology L a b . ID : C 4 0 4 W S Pulp T Ser U NP S W PCB 194 2,2',3,3',4,4',5,5'-Octachlorobiphenyl 10, 15 10, 15 10, 15 15, 22 28 28 PCB 195 PCB 196 2,2',3,3',4,4',5,6-Octachlorobiphenyl 2,2',3,3',4,4',5,6-Octachlorobiphenyl 10, 15 10, 15 15 15 10, 15 15 15 15 28 28 28 28 PCB 196/203 PCB 197 2,2',3,3',4,4',6,6'-Octachlorobiphenyl 10 10 10, 15 10, 15 10 10, 15 15 28 28 PCB 198 PCB 199 2,2',3,3',4,5,5',6-Octachlorobiphenyl 2,2',3,3',4,5,5',6'-Octachlorobiphenyl 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 15 15 28 28 28 28 PCB 200 2,2',3,3',4,5,6,6'-Octachlorobiphenyl 15 15 15 15 28 28 PCB 201 PCB 202 PCB 203 PCB 204 PCB 205 2,2',3,3',4,5',6,6'-Octachlorobiphenyl 2,2',3,3',5,5',6,6'-Octachlorobiphenyl 2,2',3,4,4',5,5',6-Octachlorobiphenyl 2,2',3,4,4',5,6,6'-Octachlorobiphenyl 2,3,3',4,4',5,5',6-Octachlorobiphenyl 10, 15 15 15 15 10, 15 10, 15 15 15 15 10, 15 10, 15 15 15 15 10, 15 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 PCB 206 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 207 PCB 208 PCB 209 2,2',3,3',4,4',5,6,6'-Nonachlorobiphenyl 2,2',3,3',4,5,5',6,6'-Nonachlorobiphenyl Decachlorobiphenyl 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 15 15 15 28 28 28 28 28 28 Total Monochlorobiphenyls 15 15 15 15 28 28 Total Dichlorobiphenyls Total Trichlorobiphenyls 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 15 15 28 28 28 28 Total Tetrachlorobiphenyls Total Pentachlorobiphenyls Total Hexachlorobiphenyls Total Heptachlorobiphenyls Total Octachlorobiphenyls Total Nonachlorobiphenyls 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 15 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 28 28 Total Decachlorobiphenyls Total Polychlorinated biphenyls 10 10 10 10 10 10 Aroclors Aroclor 1260 Aroclor 1254 Aroclor 1268 Aroclor 1221 Aroclor 1232 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 ACC-101 Rev 08, 08-Nov-2012 Page 296 of 307 www.axysanalytical.com TABLE 2 Aroclor 1248 Aroclor 1016 Aroclor 1242 Aroclor 1242/1016 Pesticides 2,4'-DDD 2,4'-DDE 2,4'-DDT 4,4'-DDD 4,4'-DDE 4,4'-DDT Aldrin Alpha-HCH Beta-HCH cis-Chlordane (alpha-Chlordane) cis-Nonachlor Delta-HCH Dieldrin Endosulphan I Endosulphan II Endosulphan sulphate Endrin Endrin aldehyde Endrin ketone Gamma-HCH (Lindane) Heptachlor Heptachlor epoxide Hexachlorobenzene Hexachlorobutadiene Methoxychlor Mirex Oxychlordane Toxaphene trans-Chlordane (gamma-Chlordane) ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology A c c re d ita tio n n o .: A 2 6 3 7 L a b . ID : C 4 0 4 W S Pulp T Ser U NP W 10 10 10 S 10 10 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 Page 297 of 307 www.axysanalytical.com TABLE 2 trans-Nonachlor BDE - Brominated Diphenylethers BDE 7 2,4-dibromodiphenylether BDE 8 2,4'-dibromodiphenylether BDE 10 2,6-dibromodiphenylether BDE 11 3,3'-dibromodiphenylether BDE 12 3,4-dibromodiphenylether BDE 13 3,4'-dibromodiphenylether BDE 15 4,4'-dibromodiphenylether BDE 17 2,2',4-tribromodiphenylether BDE 25 2,3',4-tribromodiphenylether BDE 28 2,4,4'-tribromodiphenylether BDE 30 2,4,6-tribromodiphenylether BDE-33 2',3,4-tribromodiphenylether BDE 35 3,3',4-tribromodiphenylether BDE 37 3,4,4'-tribromodiphenylether BDE 47 2,2',4,4'-tetrabromodiphenylether BDE 49 2,2',4,5'-tetrabromodiphenylether BDE 66 2,3',4,4'-tetrabromodiphenylether BDE 75 2,4,4',6-tetrabromodiphenylether BDE 77 3,3',4,4'-tetrabromodiphenylether BDE 85 2,2',3,4,4'-pentabromodiphenylether BDE 99 2,2',4,4',5-pentabromodiphenylether BDE 100 2,2',4,4',6-pentabromodiphenylether BDE 105 2,3,3',4,4'-pentabromodiphenylether BDE 116 2,3,4,5,6-pentabromodiphenylether BDE 119 2,3',4,4',6-pentabromodiphenylether BDE 126 3,3',4,4',5-pentabromodiphenylether BDE 140 2,2',3,4,4',6'-hexabromodiphenylether BDE 153 2,2',4,4',5,5'-hexabromodiphenylether BDE 154 2,2',4,4',5',6-hexabromodiphenylether BDE 155 2,2',4,4',6,6'-hexabromodiphenylether BDE 166 2,3,4,4',5,6-hexabromodiphenylether BDE 181 2,2',3,4,4',5,6-heptabromodiphenylether ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) A c c re d ita tio n n o .: A 2 6 3 7 W S Pulp T Ser U 16 10, 16 10, 16 10, 16 W ashing to n State D epartm ent of Ecology L a b . ID : C 4 0 4 NP S W 16 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 Page 298 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 BDE-183 BDE 190 BDE 206 BDE 207 BDE 208 BDE 209 2,2',3,4,4',5',6-heptabromodiphenylether 2,3,3',4,4',5,6-heptabromodiphenylether 2,2',3,3',4,4',5,5',6-nonabromodiphenylether 2,2',3,3',4,4',5,6,6'-nonabromodiphenylether 2,2',3,3',4,5,5',6,6'-nonabromodiphenylether Decabromodiphenylether PFC - Perfluorinated Organic Compounds Perfluorobutanoate (PFBA) Perfluoropentanoate (PFPeA) Perfluorohexanoate (PFHxA) Perfluoroheptanoate (PFHpA) Perfluorooctanoate (PFOA) Perfluorononanoate (PFNA) Perfluorodecanoate (PFDA) Perfluoroundecanoate (PFUnA) Perfluorododecanoate (PFDoA) Perfluorobutanesulfonate (PFBS) Perfluorohexanesulfonate (PFHxS) Perfluorooctanesulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) PAH Anthracene Pyrene Benzo[ghi]perylene Benzo[e]pyrene Indeno[1,2,3-cd]pyrene Perylene Benzo[b]fluoranthene Fluoranthene Benzo[j/k]fluoranthenes Benzo[k]fluoranthene Acenaphthylene Chrysene W 17 17 17 17 17 17 14 14 14 14 14 14 14 14 14 14 14 14 14 29 29 29 29 29 29 29 29 29 29 29 29 Canadian Association for Laboratory Accreditation (CALA) A c c re d ita tio n n o .: A 2 6 3 7 S Pulp T Ser 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 29 18 29 18 29 18 29 18 29 18 29 18 29 18 29 18 18 29 18 29 18 W ashing to n State D epartm ent of Ecology L a b . ID : C 4 0 4 U NP W 17 17 17 17 17 17 S ACC-101 Rev 08, 08-Nov-2012 Page 299 of 307 www.axysanalytical.com TABLE 2 Benzo[a]pyrene D ib e n z [a h ]a n th ra c e n e B e n z [a ]a n th ra c e n e A ce n a p h th e n e P h e n a n th re n e F lu o re n e N a p h th a le n e 1-M ethylchrysene 1-M ethylnaphthalene 1-M ethylphenanthrene 1,2-Dim ethylnaphthalene 1,2,6-T rim ethylphenanthrene 1,4,6,7-Tetram ethylnaphthalene 1,7-Dim ethylfluorene 1,7-Dim ethylphenanthrene 1,8-Dim ethylphenanthrene 2-M ethylanthracene 2-M ethylfluorene 2-M ethylnaphthalene 2-M ethylphenanthrene 2/3-M ethyldibenzothiophenes 2,3,5-T rim ethylnaphthalene 2,3,6-T rim ethylnaphthalene 2,4-D im ethyldibenzothiophene 2,6-D im ethylnaphthalene 2,6-D im ethylphenanthrene 3-M ethylfluoranthene/ Benzo(a)fluorene 3-M ethylphenanthrene 3,6-D im ethylphenanthrene 5/6-M ethylchrysenes 5,9-D im ethylchrysene 7-M ethylbenzo(a)pyrene 9/4-M ethylphenanthrenes Biphenyl C 1 -A ce n a p h th e n e s ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 29 29 29 29 18 18 29 29 18 29 29 29 29 18 18 29 29 18 29 29 18 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 Page 300 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 C 1 -B e n z (a )a n th ra c e n e s /c h ry s e n e s C 1-Benzofluoranthenes/ Benzopyrenes C 1 -B ip h e n y ls C 1-Dibenzothiophene C 1 -F lu o ra n th e n e s /P y re n e s C 1 -F lu o re n e s C 1-Naphthalenes C 1 -P h e n a n th re n e s /A n th ra c e n e s C 2 -B e n z(a )a n th ra ce n e s/C h ryse n e s C 2-Benzofluoranthenes/ Benzopyrenes C 2 -B ip h e n y ls C 2-Dibenzothiophene C 2 -F lu o ra n th e n e s /P y re n e s C 2 -F lu o re n e s C 2-Naphthalenes C 2 -P h e n a n th re n e s /A n th ra c e n e s C 3 -B e n z(a )a n th ra ce n e s/C h ryse n e s C 3-Dibenzothiophene C 3 -F lu o ra n th e n e s /P y re n e s C 3 -F lu o re n e s C 3-Naphthalenes C 3 -P h e n a n th re n e s /A n th ra c e n e s C 4 -B e n z(a )a n th ra ce n e s/C h ryse n e s C 4-Dibenzothiophene C 4 -F lu o ra n th e n e s /P y re n e s C 4-Naphthalenes C 4 -P h e n a n th re n e s /A n th ra c e n e s D ib e n z o th io p h e n e Retene PPCP (Pharmaceutical and Personal Care Products) Acetam inophen Azithrom ycin C a ffe in e C a rb a d o x Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 19 19 19 19 19 19 19 19 ACC-101 Rev 08, 08-Nov-2012 Page 301 of 307 www.axysanalytical.com TABLE 2 C arbam azepine Cefotaxim e C ip ro flo x a c in Clarithrom ycin C lin a flo x a c in C lo x a c illin D e h y d ro n ife d ip in e D ig o x ig e n in Digoxin D iltia z e m 1,7-Dim ethylxanthine Diphenhydram ine E n ro flo x a c in E rythrom ycin Flum equine F lu o x e tin e Lincom ycin Lom efloxacin M iconazole N o rflo x a c in N o rge stim a te O floxacin Orm etoprim O xacillin O xolinic acid Penicillin G Penicillin V R o x ith ro m y c in Sarafloxacin S u lfa c h lo ro p y rid a z in e S u lfa d ia z in e Sulfadim ethoxine Sulfam erazine Sulfam ethazine Sulfam ethizole ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 Page 302 of 307 www.axysanalytical.com TABLE 2 Sulfam ethoxazole Sulfanilam ide Sulfathiazole T h ia b e n d a z o le Trim ethoprim T y lo s in Virginiam ycin Anhydrochlortetracycline (ACTC) A nhydrotetracycline (ATC) Chlortetracycline (CTC) Dem eclocycline D o x y c y c lin e 4-Epianhydrochlortetracycline (EACTC) 4-Epianhydrotetracycline (EATC) 4-Epichlortetracycline (ECTC) 4-Epioxytetracycline (EOTC) 4-Epitetracycline (ETC) Isochlortetracycline (ICTC) M inocycline O xytetracycline (OTC) Tetracycline (TC) Bisphenol A Furosem ide Gem fibrozil G lip iz id e G lyburide H y d ro c h lo ro th ia z id e 2 -h y d ro x y -ib u p ro fe n Ibuprofen Naproxen T ric lo c a rb a n Triclosan W arfarin ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 Page 303 of 307 www.axysanalytical.com TABLE 2 A lb u te ro l Am phetam ine A te n o lo l A to rv a s ta tin Cim etidine C lo n id in e C o d e in e C o tin in e Enalapril Hydrocodone M e tfo rm in Oxycodone R a n itid in e Triam terene A lp ra z o la m Am itriptyline Am lodipine B e n z o y le c g o n in e Benztropine Betam ethasone C o c a in e DEET (N,N-diethyl-m -toluam ide) Desm ethyldiltiazem Diazepam F lu o c in o n id e Fluticasone propionate H y d ro c o rtis o n e 10-hydroxy-am itriptyline M ep rob am ate M ethylprednisolone M etoprolol N o rflu o x e tin e N o rverap am il ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 Page 304 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 Paroxetine Prednisolone Prednisone Promethazine Propoxyphene Propranolol Sertraline Simvastatin Theophylline Trenbolone Trenbolone acetate Valsartan Verapamil HBCDD alpha-hexabromocyclododecane (a-HBCDD) beta-hexabromocyclododecane (b-HBCDD) gamma-hexabromocyclododecane (g-HBCDD) TBBPA Tetrabromobisphenol A BPA and MPE 4,4'-dihydroxy-2,2-diphenylpropane (Bisphenol A) (BPA) Mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) Mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP) Mono-(3-carboxypropyl) phthalate (MCPP) Mono-2-ethylhexyl phthalate (MEHP) Mono-benzyl phthalate (MBzP) Mono-butyl phthalate (MBP) (n + iso) Mono-cyclohexyl phthalate (MCHP) Mono-ethyl phthalate (MEP) Mono-iso-nonyl phthalate (MiNP) Mono-methyl phthalate (MMP) Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 S 24 24 24 25 26 26 26 26 26 26 26 26 26 26 26 ACC-101 Rev 08, 08-Nov-2012 Page 305 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W Parabens Benzylparaben (Benzyl 4-hydroxybenzoate) Butylparaben (n-Butyl 4-hydroxybenzoate and isobutyl 4hydroxybenzoate) Ethylparaben (Ethyl 4-hydroxybenzoate) Isopropylparaben (Isopropyl 4-hydroxybenzoate) Methylparaben (Methyl 4-hydroxybenzoate) n-Propylparaben (Propyl 4-hydroxybenzoate) 27 27 27 27 27 27 Table 1 and Table 2 - Explanation of Terms Used: NELAP = National Environmental Laboratory Accreditation Program Non-potable water = water not fit for consumption without treatment as it may contain pollutants, contaminants, minerals or infective agents. Surface water, ground water, rainwater, effluents as well as any other non-drinking water sources are included in this category. Solid = environmental solid sample. Soil, sediment, biosolids, hazardous waste, mixed phase samples with significant solids content are included in this category. Performance based implementation = methodology follows that of the method reference but modifications deemed by AXYS as minor 1may apply, results meet method reference data quality standard. Performance based modification = modifications deemed by AXYS as significant2 have been made to method reference protocol, results meet method reference accuracy standard. The suitability of the methodology for any method prescriptive applications should be assessed based on the modifications made and the specific work requirements. Performance based method = an in-house AXYS method, published method reference not applicable. GC/LRMS = gas chromatography, low resolution mass spectrometry detection. GC/HRMS = gas chromatography, high resolution mass spectrometry detection. GC/ECD = gas chromatography, electron capture detection. LC/MS-MS = liquid chromatography, mass spectrometry-mass spectrometry detection. ACC-101 Rev 08, 08-Nov-2012 Page 306 of 307 www.axysanalytical.com AXYS Analytical Services Ltd. Note 1: Performance Based Implementation - Examples of Minor Modifications - use of additional isotopically labeled references - adjustment of calibration range - adjustment of clean-up technique - use of a different extraction of same general type (example soxhlet vs soxhlet Dean Stark) - addition of matrix type using same principles (example addition of tissue matrix using same detection principle and similar extraction type) Note 2: Performance Based Modification - Examples of Significant Modifications - different acquisition conditions using same detection principle (example MS SIM vs. full scan) - different internal control limits while meeting method reference accuracy standard ACC-101 Rev 08, 08-Nov-2012 Page 307 of 307 www.axysanalytical.com >)][( A X Y S A xys A n a ly tic a l S e rvices Ltd 2045 Mills Road West SIDNEY, BRITISH COLUMBIA, CANADA V8L 5X2 TEL 250-655-5800 FAX 250-655-5811 w w w .a x y s a n a ly tic a l.c o m AXYS Client No.: Client Address: 4731 Weston Solutions Inc. 1400 Weston Way West Chester, PA, US, 19380 The AXYS contact for these data is Candice Navaroli. Page 1 of 234 www.axysanalytical.com PERFLUORINATED ORGANIC ANALYSIS TISSUE SAMPLES PROJECT NAME: Fall 2012 Tennessee River Sampling Contract: 4731 Data Package Identification: DPWG42640 Analysis WG42409 1 February 2013 Page 2 of 234 www.axysanalytical.com WESTON SOLUTIONS TISSUE SAMPLES PERFLUORINATED ORGANIC ANALYSIS AXYS METHOD: MLA-060 PROJECT NAME: Fall 2012 Tennessee River Sampling 4731: L19064-1 to -16 NARRATIVE 1 February 2013 This narrative describes the analysis of sixteen tissue samples for the determination of perfluorinated organic compounds using high performance liquid chromatography/tandem mass spectrometry (HPLC/MSMS). SAMPLE RECEIPT AND STORAGE The samples were received on the 8thof January 2013. Details of sample conditions on receipt are provided on the sample receiving records included with this data package. The samples were stored at -20C prior to extraction and analysis. SAMPLE PREPARATION AND ANALYSIS Sample extraction, instrumental analysis and analyte quantification procedures were in accordance with AXYS Method MLA-043: A n a l y t i c a l P r o c e d u r e f o r t h e A n a l y s i s o f P e r f lu o r i n a t e d O r g a n ic C o m p o u n d s in T is s u e S a m p le s b y L C -M S /M S . Approximately 2g (wet) of each sample was accurately weighed, spiked with labeled quantification standards and extracted in basic methanol. After extraction, the raw extract was gravimetrically split into two equal portions: a 1/2 portion for cleanup and a 1/2 portion for backup. The portion of extract for cleanup was spiked with labeled cleanup standards and then cleaned up using SPE cartridge and carbon. The extracts were then spiked with labelled recovery (internal) standards, and analyzed using liquid chromatography/mass spectrometry (LC-MS/MS). Sample MSF054 (AXYS ID: L19064-14) was analyzed in duplicate. The Duplicate was assigned the AXYS ID WG42409-103. CALCULATIONS Target analyte concentrations were determined using the isotope dilution/internal standard method, comparing the area of the quantification of the target analyte to that of the labeled quantification standard (surrogate) and correcting for response factors. Linear regression equations were determined from a multi point calibration series with 1/X2 weighting fit. Labeled analyte recovery was determined by using average response factors determined from the initial multipoint calibration and were used as general data quality indicators. Recovery values of the recovery (injection) standard were estimated by external standard quantification and used as indicators of the extent of signal suppression or enhancement. Sample specific detection limits (SDL) were determined from the analysis data by converting the area equivalent to 3x the chromatographic noise to a concentration in the same manner that peak areas are converted to concentrations. Formulae used for the conversion of the raw chromatogram data to concentration are provided in the method summary document, MSU-060. REPORTING CONVENTIONS For internal tracking, AXYS assigned Weston Solutions Inc. contract number 4731. AXYS logged the samples under unique laboratory identifier L19064-X; where X is a numeral. All data reports provided reference both the Axys ID and the client sample identifier. To assist in locating data, a table correlating Axys IDs with the client sample numbers is included with this data package. The report forms were generated using Laboratory Information Management Software (LIMS). Page 3 of 234 www.axysanalytical.com generated using Laboratory Information Management Software (LIMS). Any additional work performed after the initial instrumental analysis of the sample extract is indicated by a suffix added to the AXYS ID. The notation used to identify samples reported from extra work data or that were used as the duplicate of a client sample for this data package includes: (A) = the parent sample for a duplicate pair The following data qualifier flags were used in this data package: U = identifies a compound that was not detected V = surrogate recovery is not within method control limits The reporting limit (RL) was defined as the concentration equivalent to the lowest calibration standard analyzed in the initial calibration (CSO), corrected for sample size, or the sample specific detection limits, whichever was greater. Sample analyte concentrations are reported in units of nanograms per gram (ng/g) on a wet weight basis. Results are reported to three significant figures. QA/QC NOTES The laboratory procedural blank, OPR, and duplicate samples were prepared alongside the client samples, and were subjected to the same analytical procedures. The results were evaluated through comparison to the batch QC samples. Sample analyte concentrations are not blank corrected and should be evaluated with consideration of the laboratory procedural blank results. By virtue of the isotope dilution standard quantification procedures, data are recovery corrected for possible losses during extraction and clean-up. The Signal/Noise (S/N) ratios were measured as `0' for some compounds in the QC samples and sample data. This has been determined to be a limitation of the software and does not affect the data All linearity, calibration verification, OPR and labelled compound recovery specifications were met with the following exception: The recovery of 13C4-PFBA and /or 13C2-PFHxA in samples MSF126, MSF054, the Lab Blank and Duplicate (AXYS ID: L19064-11, -14, WG42409-101 and -103) did not meet the method criteria; these compounds are flagged with a `V'. As the isotope dilution method of quantification produces data that are recovery corrected, the slight variances from the method acceptance criteria are deemed not to affect the quantification of these analytes. Percent surrogate recoveries are used as general method performance indicator only. ANALYTICAL DISCUSSION No analytical difficulties were encountered for this analysis. Page 4 of 234 www.axysanalytical.com DATA PACKAGE This data package is assigned a unique identifier, DPWG42640, shown on the front page. The following documents are included in the data package: Method summary Sample Correlation Table Sample Receiving Documentation Laboratory extraction Worksheets Sample data reports (in order of Axys Sample ID) Laboratory QC data reports Instrumental QC data reports Laboratory QC and sample raw data Instrumental QC raw data Accreditation Scope I certify that this data package is in compliance with the terms and conditions of the contract, both technically and for completeness, except for the conditions detailed above. In addition, I certify that to the best of my knowledge and belief the data as reported are true and accurate. The following signature authorizes on behalf of Axys Analytical Services Ltd the release of the data contained in this data package. Signed: Kristina Coleman, Data Validation Chemist Date Signed m Page 5 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Summary of AXYS Method MLA-043 Rev 08 Ver 03: Analytical Procedure for the Analysis of Perfluorinated Organic Com pounds in Tissue Samples by LC-MS/MS AXYS method MLA-043 describes the analysis of perfluorinated organic compounds (PFC) in tissue samples. Typical detection limits are in the range of 0.5 - 1 ng/g for 2 g samples. Target Analytes Perfluorobutanoate (PFBA) Perfluoropentanoate (PFPeA) Perfluorohexanoate (PFHxA) Perfluoroheptanoate (PFHpA) Perfluorooctanoate (PFOA) Perfluorononanoate (PFNA) Perfluorodecanoate (PFDA) Perfluoroundecanoate (PFUnA) Perfluorododecanoate (PFDoA) Perfluorobutanesulfonate (PFBS) Perfluorohexanesulfonate (PFHxS) Perfluorooctanesulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) EXTRACTION The default sample size is 2 g (wet weight). Surrogate standards are added and the sample is extracted by shaking with methanolic potassium hydroxide solution. COLUMN CHROMATOGRAPHY CLEANUP After centrifugation a suitable aliquot of the supernatant is diluted with water and cleaned up by solid phase extraction (SPE) using disposable cartridges containing a weak anion exchange sorbent. Optional cleanup may be performed with ultra pure carbon powder. The eluate is spiked with recovery standards and analyzed by LC-MS/MS. Calibration solutions are processed through the same SPE procedure. INSTRUMENTAL ANALYSIS Analysis of sample extracts for perfluorinated organics by LC-MS/MS is performed on a high performance liquid chromatograph coupled to a triple quadrupole mass spectrometer. The MS is run at unit mass resolution in the Multiple Reaction Monitoring (MRM) mode. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 1 of 6 Page 6 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Analytes, Ions, and Quantification References Target Analyte Perfluorobutanoate (PFBA) Perfluoropentanoate (PFPeA) Perfluorohexanoate (PFHxA) Perfluoroheptanoate (PFHpA) Perfluorooctanoate (PFOA) Perfluorononanoate (PFNA) Perfluorodecanoate (PFDA) Perfluoroundecanoate (PFUnA) Perfluorododecanoate (PFDoA) Perfluorobutane sulfonate (PFBS) Perfluorohexane sulphonate (PFHxS) Perfluorooctane sulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) Surrogate Standard 13C4-Perfluorobutanoic acid (13C4-PFBA) 13C2-Perfluorohexanoic acid (13C2-PFHxA) 13C2-Perfluorooctanoic acid (13C2-PFOA) 13C5-Perfluorononanoic acid (13C5-PFNA) 13C2-Perfluorodecanoic acid (13C2-PFDA) 13C2-Perfluorododecanoic acid (13C2-PFDoA) 18O2-Perfluorohexanesulfonate (18O2-PFHxS) 13C4-Perfluorooctanesulfonate (13C4-PFOS) 13C8-Perfluorooctane sulfonamide (13C8-PFOSA) Clean-up Standard 13C8-Perfluorooctanoic acid (13C8-PFOA) Recovery Standard 13C2-2H-Perfluoro-2-decenoic acid (13C2-PFOUEA) 13C4-Perfluorooctanoic acid (13C4-PFOA) Typical Retention Time (minutes) 5.0 5.8 6.2 6.6 7.0 7.4 7.9 8.5 9.0 6.3 7.2 8.2 9.9 5.0 6.2 7.0 7.4 7.9 9.0 7.2 8.2 9.9 7.0 7.3 6.9 Parent Ion Mass 213 263 313 363 413 463 513 563 613 299 399 499 498 217 315 415 468 515 615 403 503 506 421 459 417 Daughter Quantified Ion Mass Against 169 13c 4- p f b a 219 13C 2 -PFHxA 269 13C 2-PFHxA 319 369 (169)1 419 13C 2 -PFHxA 13C 2-PFO A 13C 5-PFNA 469 13C 2-PFDA 519 13c 2- p f d a 569 80 (99)1 80 (99/119)1 80 (99)1 78 13C2 -PFDoA 18O 2 -PFHxS 18O 2 -PFHxS 13c 4- p f o s 13C b-PFO SA 172 270 370 423 470 570 84 (103)1 80 (99)1 78 13c 4-p f o a 13c 4-p f o a 376 394 372 - Alternate transition within brackets, may be used if necessary to avoid interference. << 0 s s0 IL IL LL LL CL CL CL CL 1111 CoO CoO CoO CoO s LL CL 1 CoO ss LL LL CL CL 11 CoO CoO s LL CL 1 CoO MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 2 of 6 Page 7 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. CALIBRATION A series of at least six calibration solutions prepared in a clean reference tissue matrix similar in composition to the sample extract is used to establish initial multi-level calibration. The calibration solutions contain the analytes of interest covering the working range of the instrument together with labelled surrogate and recovery standards. A mid-level calibration solution is analyzed at least after every 20th sample to demonstrate calibration stability. All calibration solutions are processed through SPE cleanup. The concentration of the lowest level calibration standard used for quantification must exceed the method detection limit (MDL). Nominal Final Concentrations of Calibration Solutions (ng/mL)1 PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 13c 4- p f b a 13C 2 -PFHxA 13C 2-PFO A 13c 5- p f n a 13c 2- p f d a 13C 2-PFDoA 18O 2 -PFH xS 13C 4-P FO S 13c 8- p f o s a 13c 2- p f o u e a 13c 4- p f o a CAL A 0 .1 2 5 0 .1 2 5 0 .1 2 5 0 .1 2 5 0 .1 2 5 0 .1 2 5 0 .1 2 5 0 .1 2 5 0 .1 2 5 0 .2 5 0 .2 5 0 .2 5 0 .1 2 5 3 3 9 3 3 3 4 .5 4 .5 3 2 .5 3 CAL B 0 .3 1 2 0 .3 1 2 0 .3 1 2 0 .3 1 2 0 .3 1 2 0 .3 1 2 0 .3 1 2 0 .3 1 2 0 .3 1 2 0 .6 2 5 0 .6 2 5 0 .6 2 5 0 .3 1 2 3 3 9 3 3 3 4 .5 4 .5 3 2 .5 3 CAL C 1 .2 5 1 .2 5 1 .2 5 1 .2 5 1 .2 5 1 .2 5 1 .2 5 1 .2 5 1 .2 5 2 .5 2 .5 2 .5 1 .2 5 3 3 9 3 3 3 4 .5 4 .5 3 2 .5 3 CAL D 5 5 5 5 5 5 5 5 5 10 10 10 5 3 3 9 3 3 3 4 .5 4 .5 3 2 .5 3 CAL E 25 25 25 25 25 25 25 25 25 50 50 50 25 3 3 9 3 3 3 4 .5 4 .5 3 2 .5 3 CAL F 50 50 50 50 50 50 50 50 50 100 100 100 50 3 3 9 3 3 3 4 .5 4 .5 3 2 .5 3 CAL G CAL H 125 125 125 125 125 125 125 125 125 250 250 250 125 3 3 9 3 3 3 4 .5 4 .5 3 2 .5 3 3 1 2 .5 3 1 2 .5 3 1 2 .5 3 1 2 .5 3 1 2 .5 3 1 2 .5 3 1 2 .5 3 1 2 .5 3 1 2 .5 625 625 625 3 1 2 .5 3 3 9 3 3 3 4 .5 4 .5 3 2 .5 3 1 C o n c e n tra tio n s sho w n are based on th e a m o u n t ad ded to c a lib ra tio n sta n d a rd s, w ith a p p ro p ria te sp lit po rtion (1/10, 1 /2 o r e n tire e x tra c t) c le a n e d up on S P E a n d c a lc u la te d in te rm s o f a 4 m L e x tra c t v o lu m e . MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 3 of 6 Page 8 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. ANALYTE IDENTIFICATION Positive identification of target PFC, surrogate standard and recovery standards require: > 3:1 S:N for parent ion to daughter ion transition. Compound retention time must fall within 0.4 minutes of the predicted retention times from the daily Calibration Verification. Natives with labeled surrogate standards must elute within 0.1 minutes of the associated labeled surrogates. QUANTIFICATION Target compounds are quantified using the internal standard method, comparing the area of the quantification ion to that of the 13C-labelled standard and correcting for response factors. Linear quantification equations are determined from a multi-point calibration series with 1/X2 weighting fit and expressed as below: Y = slope x X + intercept Where: Y = response ratio area of Target x weight of Surr (ng) area of Surr X = weight of target (ng) , and The slope and intercept are used to convert raw peak areas in sample chromatograms to final concentrations as follows: Sample Conc. area of Target 1 ar 1a x weight of Surr (ng) - interceptj x x area of Surr slope j Vsample size (g) j where Surr is the surrogate standard The recovery of the surrogate standard is calculated (by internal standard quantification against the recovery standard using an average RRF) and monitored as an indication of overall data quality. Final target concentrations are recovery corrected by this method of quantification. REPORTING LIMITS Concentrations and detection limits for the target analytes are reported. Typical reporting units for all data are ng/g on a wet weight basis. The following are commonly requested reporting limits: Method Detection Limit (MDL) - determined as specified by EPA Fed. Reg. 40 CFR Part 136 Appendix B (no iteration option). The 99% confidence level MDL is determined based on analysis of a minimum of 7 replicate matrix spikes fortified at 1-5 times the estimated detection limit. MDL is determined as required based on accreditation, contract and workload requirements. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 4 of 6 Page 9 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Lower Method Calibration Limit (LMCL) - determined by prorating the concentration of the lowest calibration limit for sample size and extract volume. The following equation is used. LMCL = ((lowest level cal conc.) x (extract volume))/sample size. Typical extract volume for PFCs in tissues is 4 mL. For the analysis of PFCs it is AXYS standard to report sample concentrations using the LMCL as the lower reporting limit. In cases where the SDL is higher than the LMCL, the SDL will be used as the lower reporting limit. The SDL is defined as follows: Sample Specific Detection Limit or Sample Detection Limit (SDL) - determined individually for every sample analysis run by converting the area equivalent of 3.0 times (2.5 times for EPA 1600 series methods) the estimated chromatographic noise height to a concentration in the same manner that target peak responses are converted to final concentrations. The SDL accounts for any effect of matrix on the detection system and for recovery achieved through the analytical work-up. QUALITY ASSURANCE / QUALITY CONTROL All samples are analyzed in batches. The composition of a batch is detailed on a batch sheet. Each batch has the following composition: Batch Size - Each batch consists of test samples and additional QC samples. Blanks - 5% of the samples within a batch are procedural blanks. Duplicates - 5% of the samples within a batch are analyzed in duplicate. Matrix Spike/Matrix Spike Duplicate - may be analyzed upon client request. Reference Samples - 5% of the samples within a batch are spiked reference samples. QC Specification Table: Procedural Blank Levels and OPR Recoveries Analyte Perfluorobutanoate Perfluoropentanoate Perfluorohexanoate Perfluoroheptanoate Perfluorooctanoate Perfluorononanoate Perfluorodecanoate Perfluoroundecanoate Perfluorododecanoate Perfluorobutanesulfonate Perfluorohexanesulfonate Perfluorooctanesulfonate Perfluorooctane sulfonamide (PFBA) (PFPeA) (PFHxA) (PFHpA) (PFOA) (PFNA) (PFDA) (PFUnA) (PFDoA) (PFBS) (PFHxS) (PFOS) (PFOSA) Procedural Blank Level ng/g 1 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 <1 <1 <1 < 0.5 Acceptable Matrix Spike (% Recovery) 2 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 1 Based on the default Option B extraction procedure a (2 gram sam ple sizesplit 2X). Procedural blank limits m ust be prorated accordingly for O ption A or C. 2 Marginal exceedance allowance - recovery for one com pound may be 60 - 140%. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 5 of 6 Page 10 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. QC Specification Table: Surrogate Standard Recoveries, Calibration and Samples Surrogate Standard 1 Recovery Range 13C4 -Perfluorobutyric acid (13C4-PFBA) 20 - 150% 13C2-Perfluorocaproic acid (13C2-PFHxA) 40 - 150% 13C2-Perfluorooctanoic acid (13C2-PFOA) 40 - 150% 13C5-Perfluorononanoic acid (13C5-PFNA) 40 - 150% 13C2-Perfluorodecanoic acid (13C2-PFDA) 40 - 150% 13C2-Perfluorododecanoic acid (13C2 -PFDoA) 40 - 150% 18O2-Perfluorohexanesulfonate (18O2 -PFHxS) 40 - 150% 13C4-Perfluorooctanesulfonate (13C4-PFOS) 40 - 150% 13C8-Perfluorooctane sulfonamide (13C8-PFOSA) 20 - 130% Clean-up Standard 13C8-Perfluorooctanoic acid (13C8-PFOA) 40 - 150% 1 Lower surrogate recoveries may be reported for individual sam ples where dilution analysis or spiked sam ple results dem onstrate acceptable accuracy. QC Specification Table: Other Parameters QC Parameter Specification Instrument Sensitivity Initial Calibration (native compounds) Daily, S:N >3:1 for all analytes for lowest calibration standard. - Daily, (1/x2) weighed linear regression. - For the calculated concentrations, a maximum of 1 point per calibration solution with a maximum of 3 points for all the calibration solutions may fall within 60-140% of the actual con centration provided all remaining points, including those for PFOA, PFOS, and PFDA, fall within 70-130% of actual concent ration. - Internal guideline - correlation coefficient >0.95. Calibration curves with lower correlation coefficient values meeting all above criteria may be accepted based on batch specific QC results and professional judgement. - Surrogate recoveries must fall within the same limits as for the samples in the table above. Continuing Calibration Verification (native compounds) Every 20 samples, determined concentrations must be within 30% of actual concentrations. Surrogate recoveries must fall within the same limits as for the samples in the table above. Instrumental Carryover and Instrument Background Every Initial Calibration, Cal/Ver, or SPM: < 0.3% carryover and area response of analytes in instrument blank < 800 judged following two previous methanol blank injections. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 6 of 6 Page 11 of 234 www.axysanalytical.com Weston Solutions Inc. CORRELATION TABLE PERFLUORINATED ORGANIC ANALYSIS Lab Name: AXYS Analytical Services Ltd. Project Name:Fall 2012 Tennessee River Sampling Data Package Identification: DPWG42640 Project Manager: Candice Navaroli Contract No: 4731 AXYS Method: MLA-043 Program: Tissue Samples Client Sample No. Lab Sample ID LAB BLANK OPR WG42409-101 WG42409-102 MSF088 MSF093 MSF008 IPF019 MSF032 IPF150 IPF154 IPF156 MSF171 MSF110 MSF126 IPF136 IPF182 MSF054 MSF064 IPF072 L19064-1 L19064-2 L19064-3 L19064-4 L19064-5 L19064-6 L19064-7 L19064-8 L19064-9 L19064-10 L19064-11 L19064-12 L19064-13 L19064-14 WG42409-103 DUPLICATE L19064-15 L19064-16 Page 12 of 234 www.axysanalytical.com Client Inform ation (Sub C ontract Lab) Client Contact: Shipping/Receiving Company: AXYS Analytical Services Ltd. Address: PO BOX 2219. 2045 Mills Road, City: Sidney State, Zip: BC, V8L 5X2 Email: Project Name: Fish Tissue Homogenization Sample Identification - Client ID (Lab ID) MSF088 (200-14181-16) MSF093 (200-14181-22) L-)iotoM - \ -a . Sampler: _ Phone: ' Due Date Requested: 12/26/2012 TAT Requested (days): WO#: Project #: 20004362 ssom. m \Chain of Custody Record TestAmerica Lab PM: Young, Kirk F E-Mail: kirk.young@testamericainc.com Carrier Tracking No(s): Sp M - H C C r- 4 4 a s ~ A nalysis R equested COC No: 200-11159.1 Page; Page 1 of 1 Job#: 200-14181-1 Preservation Codes: . A - HGL B - NaOH C - Zn Acetate D - Nitric Acid E - NaHS.04. F - MeOH G - Amchlor H - Ascorbic Acid i - Ice J - Di Water K-.EDTA L -E D A M - Hexane N - None O - AsNa02 P - Na204S Q - Na2S03 R -Na2S2S03 S-H2S04 T - TSP Dodecahydrate U - Acetone V - MCAA W - ph 4-5 Z - other (specify) Other: Sample Date 11/9/12 Sample Time Eastern Sample Type {C=Comp, G^grab) M a trix (w = w a te r, S=slid, 0= w a s te /o ii, BT=Tissue, A " Alr! Preservation ( ZK Tissue 11/15/12 Eastern Tissue Special Instructions/Note: Shippment of samples to 3M Corporation. Shippment of samples to 3M Corporation. Possible Hazard Identification Unconfirmed Deliverable Requested: I, II, III, IV, Other (specify) Empty Kit Relinquished by: Relinquished by: / /\ y \ Relinquished by; -------- fc y n C - Relinquished by: Custody Seals Intact: Custody Seal No.: A Yes A No Date/Time: ' . lR ll3 Date/Time: Date: W 5 Date/Time: . Company T4- Company Company Sample Disposal ( A fee maybe assessed if samples are retained longer than 1 month) ^~~^Return To Client______ isposal By Lab Special Instructions/QC Requirements: Archive For Months Time: RecatfgQ Received by; Method of Shipment: Date/Time: * < 0 ^ ~ \0 4 -v _ - Date/Time: ! Company Company Received by: Date/Time: Company Cooler Temperature(s) C and Other Remarks www.axysanalytical.com Client Information (Sub Contract Lab) Client Contact: ' Shtpping/Rceiving C Company: AXYS Analytical Services Ltd. Address: PO BOX 2219, 2045 Mills Road, City: Sidney State, Zip; BC, V 8 L 5X2 . ' ' Phone: Email: - Project Name: Fish Tissue Homogenization S i t e : ........................... '' " Sam ple Identification - Client ID (Lab ID) MSF008 (200-14174-8) IPF019 (200-14174-20) MSF032 (200-14174-33) L \B - H' .^ ~ Chain of Custody Record r m TestAm erica Sampler: Phone; . Due Date Requested: 12/26/2012 TAT Requested (days): PO #: WO #: Project#: 20004362 SSOW#: Sample Sample Date Time 11/3/12 11/3/12 11/3/12 Eastern Eastern Eastern Lab PM: Young, Kirk F E-Mail: k irk .y un g @ te sta m e ric a in c.co m Carrier 7 racking No{s) H H oS- Analysis Requested COC No: 200-11013.1 Page: Page 1 of 1 Job#: 200-14174-1 Preservation Codes: ill JS n 11 z a> 1 j j V, a </3 Sample Type (C =com p, G=grab) M a trix do (w=water, S = so lid , 0=waste/oil, iZ g n HI - 1issue, A=Alr) I 3f 1 Imt CL Preservistion Code: S y 1 Tissue X Tissue X Tissue X SUBCONTRACT B-NaOH C - Zn Acetate D - Nitric Acid E-NaHS04. F-MeOH G - Amchlor H - Ascorbic Acid ! - ice P J - Di Water oc ttii L - EDA N - None O - AsNa02 P - Na204S Q - Na2S03 S - H2S04 U - Acetone V -M C A A W r- ph 4-5 Z - other (specify) 0 a Other: 'o o 6 z filf o Special Instructions/Note: . Shippment of samples to 3M Corporation. 1 1 Shippment of samples to 3M Corporation. Shippment of samples to 3M Corporation. 1 Possible Hazard Identification U nconfirm ed Deliverable Requested: 1, II, III, IV, Other (specify) Empty Kit Relinquished by: Relinquished W yf ( C '7 ^ Relinquished by: E v-T g Relinquished by: . Custody Seals Intact: Custody Seal No.' A Yes A No , Date: Date/Time: Date/Time: iH I S ' Date/Time: Company TA Company Company Sample D isposal ( A fee may be assessed if sam ples are retained longer than 1 month) R eturn To C lie n t ^~ ~ bi$posal B y Lab Special Instructions/QC Requirements: ^Archive F o r M onths Time: R e ce ive d b ^^^^^^ ^ Received by: IMethod of Shipment: _____ Date/Time: t2 < g - Date/Time: Company , Company Received by: Date/Time: Company Cooler Temperature(s) C and Other Remarks m "Page 14 j f 234 www.axysanalytical.com Chain of Custody Record T e s tA m e ric a . Client Information (Sub Contract Lab) Client Contact: S h ip p in g /R e c e iv in g Company: AXYS Analytical Services Ltd. Address: PO BOX 2219, 2045 Mills Road, City: Sidney State, Zip: BC, V8L 5X2 Phone: t Email: ' . Project Name: Fish Tissue Flomogenization Site: ' Sam ple Id e n tifica tio n - C lie n t ID (Lab ID) IPF150 (200-14182-6) IPF154 (200-14182-10) IPF156 (200-14182-12) MSF171 (200-14182-28) L \S O (> C -n -8 - i Sampler: Phone: Lab PM: Young, Kirk F E-Mail: kirk.young@testamericainc.com Carrier Tracking No(s): io W i ' i L c\ S ............. MROS' COC No: 200-11159.1 Page: . Page 1 of 1 '- Analysis Requested Job#: 200-14182-1 Due Date Requested: 12/26/2012 TAT Requested (days): , PO#: ' WO#: Project #: 20004362 s s o m-. . S a m p le S a m p le Type (C=Comp, . M a trix (w=water, S=solid, 0=waste/oil, 0 z 0 tVfl V a> m, E V> TJ 1 U.. Sample Date T im e G=grab) BT=Tssue, A=Alr) Preserve X ^ P e rfo rm M S /M S D (Yes or No) SUBCONTRACT Preservation Codes: A -H C L B-NaOH C-.Zn Acetate D - Nitric Acid E - NaHSCM F-MeOH G - Amchlor H - Ascorbic Acid . f - Ice i.p J - D I Water K -EDTA 3AC'S L - EDA Co ;v. Other: m a4> M - Hexane N-None 0 -A sN a 0 2 P - Na204S Q - Na2S03 R - Na2S2S03 S - H2S04 T - TSP Dodecahydrate U -Acetone V -M C A A ' W - ph 4-5 Z - other (specify) Z f i Special Instructions/N ote: X 11/21/12 Eastern Tissue X Shippment of samples to 3M Corporation. 1 11/21/12 Eastern Tissue X Shippment of samples to 3M Corporation, 1 11/21/12 - Eastern Tissue X Shippment of samples to 3M Corporation. 1 11/29/12 Eastern Tissue X Shippment of samples to 3M Corporation. 1 Possible Hazard Identification U nconfirm ed Deliverable Requested: I, II, III, IV, Other (specify) Empty Kit Relinquished by: Relinquished by: Relinquished by: Relinquished by: .. Custody Seals Intact: A Yes A No Custody Seal No.: Date/Time: L M Z l. Date: M Sample D isposal ( A fee m ay be asse sse d if sam ples are retained longer than 1 month) ^~~^R eturn To C lie n t_____ ^ ^ D is p o s a l B y L a b ______^ ~ k r c h iv e F o r M onths Special Instructlons/QC Requirements: Time: Company iA - u /C Company Received by: Method of Shipment: 'CUC/ t HMC. . W Date/Time*^ - s^v Company Company Company Received by: Date/Time: Company Cooler Temperature^) C and Other Remarks: www.axysanalytical.com C lient inform ation (Su b Contract Lab) Client Contact: . Shipping/Receiving Company: AXYS Analytical Services Ltd. Address; PO BOX 2219, 2045 Mills Road, City: . Sidney State,.Zip: BC, V8L 5X2 Phone: Email; Project Name: Fish Tissue Homogenization Site: ' Sample Identification - Client ID (Lab ID) MSF110 (200-14185-2) MSF126 (200-14185-18) IPF136 (200-14185-29) 1PF182 (200-14185-41) L -H O b M '" lO ~ !| -(3 . -- Chain of Custody Record Ln-5 \ TestAm erica Sampler: Phone: Due Date Requested: 12/26/2012 TAT Requested (days): PO#: WO #: Project #: 20004362 SSOW#: Sample Sample Date Time "11/15/12 11/16/12 11/21/12 12/13/12 Eastern Eastern Eastern Eastern Lab PM: Young, Kirk F E-MaiS: kirk.young@testamericainc.com . Carrier Tracking No(s): S D 3 % c \s A nalysis Requested eoe No: 200-11159,1 H H o S ~ Page: Page 1 of 1 Job#: , 2 0 0 -1 4 1 8 5 -1 Preservation Codes: . B- NaOH C -Z n Acetate D - Nitric Acid E - NaHS04 . N - None 0-A sN a02 P - Na204S Q- Na2S03 'W . o t'HS" c 111 yj <&109 & X* Sample Type (C=comp, G=grab) Matrix (w=water, S=solld, 0=waste/oH, BT=Ttssue,AAlr) XI3 % il 33 a* IT m P 1 So--' 0..- ii ti X Tissue X Tissue X Tissue X Tissue X SUBCONTRACT G ~Amchlor H- Ascorbic Add I- ice to J - D l Water K-EDTA 75 L-E D A . C o Other: -S H 2 S 0 4 . T - TSP Dodecahydrate U - Acetone V-MCAA. W-.ph 4 -5 Z - other (spedfy) , z3. ; hO* Special Instructions/Note: 1 Shippment of samples o 3M Corporation. 1 Shippment of samples to 3M Corporation. 1 Shippment of samples to 3M Corporation. 1 Shippment of samples to 3M Corporation. Possible Hazard Identification U nconfirm ed Deliverable Requested: I, II, III, IV, Other (specify) Empty Kit Relinquished by: Relinquished by: / Relinquished by: , ric~ v Relinquished by: Custody Seals intact: Custody Seal No.: A Yes A No Date/Time: Date/Time: Date/Time: Date: Company Company Company Sample Disposal ( A fee maybe assessed if samples are retained longer than 1 month) ' ^ R e tu rn To C lie n t_____ bis p o s a l B y L a b ________* ^Archive F o r M onths Special Instructions/QC Requirements: Time: Recived by: W. Received by: Method of Shipment: . Date/Time: Company 'O'h - \o>ov - \S Date/Time: --< J Company Received by: Cooler Temperature(s) C and Other Remarks. ........ Date/Time: - Company _______ a Y P a u t: 10 u www.axysanalytical.com Client Information (Sub Contract Lab) Client Contact: Shipping/Receiving Company: AXYS Analytical Services Ltd. Address: . PO BOX 2219, 2045 Mills Road, City: Sidney State, Zip: BC, V8L 5X2 Phone: Email: Project Name; Fish Tissue Homogenization Site:. Sample Identification - Client ID (Lab ID) . " "" MSF054 (200-14179-18) L W c f o 1! - \ 4 MSF064 (200-14179-29) -- IPF072 (200-14179-37) _ . Sampler: ` Phone: - Due Date Requested: 12/26/2012 TAT Requested (days): Chain of Custody Record MT5 ( Lab PM: Young, Kirk F E-Mail: kirk.young@testamericainc.com Carrier Tracking No{s): Analysis Requested SUBCONTRACT PO #: WO#: Project-#: 20004362 SSOW#: Sample Sample Date Time 'O X 11/7/12 S ><C Eastern - o' Z o Ut Z ` ' m & a> Q I p$ &> f(S3 Vi ":W m T3 $ Sample M a trix ,6 l i 1 Si ! Type (C=omp, (w=water, S-soiid, 0=waste/oil, il i 2 o X G=grab) BT=Tissue,A=Air) $. mm m Preseivat on Code E X Tissue X 11/7/12 Eastern Tissue X 11/9/12 Eastern Tissue X TestAm erica COC No: 200-11013.1 Page: Page 1 of 1 Job#: 200-14179-1 Preservation Codes: A - HCL B - NaOH C - Zn Acetate 0 - Nitric Acid E - NaHS04 F - MeOH G - Amcnlor H - Ascorbic Acid 1- Ice <13 J - Dt Water K-EDTA 3C L - EDA c oo Other: o <u Xt 3 Z M - Hexane N - None O - AsNa02 P - Na204S Q - Na2S03 R - Na2S2S03 S - H2S04 T - TSP Dodecahydrate U - Acetone V - MOAA W - ph 4-5 Z - other (specify) G Special Instructions/Note: ~ r z r = . ............ -- --------------- " Shippment of samples to 3M Corporation. 1 Shippmerit of samples to 3M Corporation, 1 Shippment of samples to 3M Corporation. 1 -. Possible Hazard Identification U nconfirm ed Deliverable Requested: I, II, III, IV, Other (specify) Empty Kit Relinquished'by: Relinquished by: Relinquished by: Custody Seals Intact: Custody Seal No. A Yes A No ( ? / e Date: ifr Company Company Sam ple D isposal ( A fee m ay be asse sse d if sam ples are retained longer than 1 month) R eturn To C lie nt ^~~b/sposa/ B y Lab ^~ k rc h iv e For M onths Special Instructions/QC Requirements: iTime: Method of Shipment: Company Received by: D a te /T im e S j Company Cooler Temperature(s) C and Other Remarks: }i --------------------------------------------------------------------------------------------------------- Page 17 I f 234 www.axysanalytical.com (iT Do Not Lift Using This Tag ORIGIN ID: BTVA <802) 923-1058 ? ^ L! ingtdn 30 COMMUNITY DRIVE InflTH BURLINGTON, VT 05403 US S I G TM SAMPLE MANAGEMENT SHIP DATE: 07J|N4,3 ACAPDT:UG0T0081990.3964L/BCAnFAEN2ef&8 DIhS:U22xUxl2 IN DRY life: ',45 KGS BILL SENDER no m m m \ _ ___ _ ocadcF avaroli ^ AXYS ANALYTICAL SERVICES LTD. PO BOX 2219 2045 MILLS ROAD SIDNEY BC V8L5X2 (CA) 655-5800 FedExPNOUt; DEPT... [)> 01 02V8L5X2 124 01 5039469544050430 FDE 297200119 007 1/1 19.9LB N PO BOX 2219 SIDNEY BC CANDICE NA Express B ' S S ' f mulitun. Iliviitloii m W 1us Prell`b'l^.5..-----------n 5039 4695 4405 XQ YYJA AM1 INTL PRIORITY1 ICE V8L 5X2 BC-CA Y V R % 1009981981191180382100503946954405 ,1 . m Page 18 of 234 www.axysanalytical.com AXYS Analytical Services Ltd SAMPLE RECEIVING RECORD Waybill : Date Shipped: AXYS Client & Contract # Project Number: [Yes)/No W jA N -1 3 4731-Weston Solutions Waybill #: 503946954405 Date/Time Received: 08-JAN-13 11:20 Receipt No: WB14271 Login Number: Received By: KSTADLER Axys Sample ID's: ] - 14 o ^1/5 I nr in hy wSe)I 0 t/fS-c-- I Signatura/ A t i l l J Condition of Shipping Contaailnneerr:: ) v c k x j ' Temperature upon Receipt: -30 Celcius samples arrived frozen on dry ice Custody Seals: Shipping Containers h fM Samples Yes^No^ ln ta ct^e )/N o Intact Yes /No Seal Numbers Yes Seal Numbers Yes /No Thermometer ID: 3266 v. Custody or Documents: Sample ID's Collection Location Date & Time Collection Collector's Name (e$)/No (Yes)/No YesSo (Y e ^ /N o Yes (So) Tracking Report /Packing List: Yes/NO) Sample Tag Numbers Sample Type Yes /NO) (Yes/No Preservative Added Yes Preservation Requested Y e s /N Sample Tags Sample Labels Sample Labels Cross Referenced to COC Sample Tags Cross Referenced to Sample Labels Sample Tags Cross Referenced to COC Comments: As p v <-- V 'le - t y f ' y e j ) `S o A v y W \ Yes / % ^e |/N o (Te^l/No Yes /No Yes /No (d e r Information Agrees Information Agrees Information Agrees (Yes /Ng! Yes /No Yes /No VO 4t>_l ba 1 \ f ^ l 3 0 CplcO --\ * - V 1 8 > 3 - U > ( Q.S sVooDvk. Qty CocA \ \ S L d s . 1~ t W p a c y w c j ) \ . iS Action Taken: Page 19 of 234 www.axysanalytical.com PPSTZ. ORU.S31 1VIN3HN0tJJ\N3 Ni U3aV31 3RL D D U 0U U V 4S 01 /e a < -i r `\ 3 in iV H 3 IS fe A p o js n j T ^ tA n T e r ic g THE LEADER IN ENVIRONMENTAL TE8TING 719544 Page 20 of 234 www.axysanalytical.com $ )][(& AXYS AXYS Analytical Services Ltd. Login Chain of Custody Report (In01) Jan. 09, 2013 02:23 P M Login Number: L19064 Account: 4731 Weston Solutions Project: Axys ID versus Client Sample Identification L19064-1 MSF088 09-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY . L19064-2 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF093 15-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-3 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF008 03-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-4 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT IPF019 03-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT Scjg/tfAUaJ <ZtJ 09 ' - /^s Page: 1 of 4 Received 08-JAN-13 Due PR 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag USD USD USD USD Page 21 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. ce AXY5 Login Chain of Custody Report (In01) Jan. 09, 2013 02:23 P M Login Number: L19064 Account: 4731 Weston Solutions Project: Axys ID versus Client Sample Identification L19064-5 MSF032 03-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-6 FC043.HALF PFC EDD , PFOS DATA PKG SAMPLE RECEIPT IPF150 21-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-7 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT IPF154 21-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-8 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT IPF156 21-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT Page: 2 of 4 Received 08-JAN-13 Due PR 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag USD USD USD USD Page 22 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. AXYS Login Chain of Custody Report (In01) Jan. 09, 2013 02:23 P M Login Number: L19064 Account: 4731 Weston Solutions Project: Axys ID versus Client Sample Identification L19064-9 MSF171 29-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D. Package ANY L19064-10 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF110 15-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-11 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF126 16-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D,Package ANY L19064-12 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT IPF136 21-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT Page: 3 of 4 Received 08-JAN-13 Due PR 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag USD USD USD USD Page 23 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. m AXYS Login Chain of Custody Report (In01) Jan. 09, 2013 02:23 P M Login Number: L19064 Account: 4731 Weston Solutions Project: Axys ID versus Client Sample Identification L19064-13 IPF182 13-DEC-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-14 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF054 07-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-15 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF064 07-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-16 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT IPF072 09-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D. Package ANY FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT Page: 4 of 4 Received 08-JAN-13 Due PR 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag USD USD USD USD Page 24 of 234 www.axysanalytical.com C andice Navaroli From: Young, Charles T, [Charles.Young@WestonSolutions.com] Sent: Wednesday, January 09, 2013 5:45 AM To: Young, Kirk; Candice Navaroli Subject: RE: Homogenized Tissue - Shipment - 01/07/2013 Just so. Thanks all. Charlie C harles T. Young Technical Director W eston Solutions, Inc. 1400 W eston Way (5-2) W est C hester, PA 193S0 (610) 701-3787 cell (610) 9 52-1093 fax (610) 701-3401 charles.young@ wostonsolut.ions.com From: Young, Kirk [ mailto:Kirk.Young@testamericainc.com] Sent: Wednesday, January 09, 2013 8:42 AM To: Candice Navaroli Cc: Young, Charles T. Subject: RE: Homogenized Tissue - Shipment - 01/07/2013 Importance: High Candice, You should use the original client identifier. For sample IPF150 (200-14182-6), the original client identifier is IPF150. I hope that this is straightforward. If there are any question, please feel free to ask. Kirk KIRK YOUNG, P.E. Project Manager TestAmerica THE LEADER IN ENVIRONMENTAL TESTING 30 Community Drive, Suite 11 South Burlington, VT 05403 Tel 802,660.1990 Direct 802.923.1017 www.testamericainc.com From: Candice Navaroli rmailto:cnavaroli@axvs.com1 Sent: Tuesday, January 08, 2013 5:16 PM To: Young, Kirk Cc: Young, Charles T. Subject: RE: Homogenized Tissue - Shipment - 01/07/2013 HI Kirk and Charles, The samples arrived this morning safe and sound at -30 degrees C. There is a sample discrepancy for each sample that occurs and I would like to know how you wish for the samples to be logged in. The sample ID tag has: IPF150 (200-14182-6) but the COC has IPF150 (200-14182-A-6). This seems to be the same for each sample where there is an A added into each of the samples IDs. Which would you prefer we use, the sample contained ID or the client COC to log in the samples? Regards, Candice 09/01/2013 Page 25 of 234 www.axysanalytical.com Candice Navaroli (B.Sc.) Project Manager Tel.: 250-655-5800 Direct: 250-655-5839 Toll free: 1-888-373-0881 Ext.5839 Fax: 250-655-5811 Email: cnavaroli@axvs.com Axys Analytical Services Ltd. 2045 Mills Road Sidney, BC. Canada, V8L 5X2 From: Candice Navaroli Sent: Tuesday, January 08, 2013 9:27 AM To: 'Young, Kirk' Cc: Young, Charles T. Subject: RE: Homogenized Tissue - Shipment - 01/07/2013 Much appreciated, thanks Kirk. . I will update you and Charles as to the condition of the samples once they are received. Regards, Candice Candice Navaroli (B.Sc.) Project Manager Tel.: 250-655-5800 Direct: 250-655-5839 Toll free: 1-888-373-0881 Ext.5839 Fax: 250-655-5811 Email: cnavaroli@axvs.com Axys Analytical Services Ltd. 2045 Mills Road Sidney, BC. Canada, V8L 5X2 From: Young, Kirk fmailto: Kirk.Younqtatestamericainc.coml Sent: Tuesday, January 08, 2013 6:37 AM To: Candice Navaroli Cc: Young, Charles T. Subject: Homogenized Tissue - Shipment - 01/07/2013 Candice, We did ship the homogenized tissue from here yesterday, January 7th, for your receipt today. The shipment was made in a single cooler under FedEx tracking number 5039-4695-4405. The attached file contains the C-O-Cs and other documentation for the shipment. Thank you for your assistance, and let me know if you should have any questions. Kirk KIRK YOUNG, P.E. Project Manager 09/01/2013 Page 26 of 234 www.axysanalytical.com TestAmerica THE". LEADER IN ENVIRONMENTAL TESTING 30 Community Drive, Suite 11 South Burlington, VT 05403 Tel 802.660.1990 Direct 802.923.1017 www.testamericainc.com CONFIDENTIALITY: This email and attachments may contain information which is confidential and proprietary. Disclosure or use of any such confidential or proprietary information without the written permission of Weston Solutions, Inc. is strictly prohibited. If you received this email in error, please notify the sender by return e-mail and delete this email from your system. Thank you. 09/01/2013 Page 27 of 234 www.axysanalytical.com w w w .axysanalytical.com BATCH LIST PART ONE B A T C H ID: W G42409 Method ID: MLA-043 R8.03 Method ID: Method ID: Analyst: Start Date: : 10-Jan-13 Batch List A uthor: SDHESI Checked By: iXFA Project Manager: Glassware Type: Glassware Treatment: Rotovap Type: 1C andice N/A N/A N/A Matrix: Sample size; Moistures required? Lipids required? Extraction Solvent: FMS Program: Save FMS discards? Tissue 2g NO NO ;SPE N/A N/A Biohazard Type: None Comments: Sam ple size - 2g AXY5 A n a lytica l Services Ltd Choose largest available sample for Dup Split option B (1/2 split) p re v io u s W G's associated w ith sam ples: Samples, Batch Surrogate, Recovery Spiking details Contract # Sample Wt Contract # Sample 4731 4731 4731 L19064-1 2g. L19064-2 ^ .... L19064-3 i 2 g 4731 4731 1731 4731 4731 LI 9064-4 .. L19064-5 .................... L1DC04-G ...2.9... 2g L19064-7 ?9 L i 0064-3 2.9. 4731 4731 L19064-9 L19064-10 ?S 2 a.... 4731 4731 L10064-11 ; L I 9064-42 . ..2 9 . 2g 4731 L19064-13 .... 2.9.... 4731 4731 4731 L19064-14 L19064-15 L19064-16 2.9. . ...2 g ..... 2 9 .... Wt S p ik in g : Product FC043.HALF FC043.HALF Before Extraction: Samples required: Surrogate_______ |L19064-1.2.3,415.B.7I8,S,10. I g , , * * f1, 12, 13, T4, 15, 16. WG42409- ; ' -'T t01; 102, 103______________I40uL FC023A-SUR/ g f :WG4249-104SARonly i s - o 3- i *7 ! 20 u L F 023A -S U R! o f ...' After Extraction: : Cleanup S urrogate Recovery__________ F.V. e t\* $4- :(gtiH ) ^ 6 ............. 20uL FCQ22A-SUR/t)2 :50uL FC009A-REC/QI 4000 uL 20uL FC22A-SUR/ :50uL FC09A-REC/ 4000 uL U-vial Instructions and Comments: PPROP SNAPWi SAR is spiked w ith 1i2 o f the SUR used in the samples. CHECK THAT UN PVCD SAIL ALSO USES 1/2 SUFt IPPROP SNAPW/ IPVCD FSA-Q04 Rev 16, 16-Feb-2010 i m Page 1 Bagfe-29 of 234 w w w .axysanalytical.com BATCH U S7 PART TWO BATCH ID: WG4249 Type and Analysis QC QC Samples Weight______ required for: FCD43 HALE Authentic spiking: WG42409-101 BLANK 'G CANADIAN SPRINGS WATER AXYS Analytical Services Ltd QC samples, Authentic Spiking arid Comments Comments: Type and Analysis QC QC Samples Weight required for: Authentic spiking: Comments: W G 424Q 9-1G 2 SPM OPT. A: 1G SC52G5 T" L500-30 (15T-REF). ! OPT, B&C; 1G SC5211 (12T-RF) !320uL FC039B-AUT7 2r WG42409-103 DUP CHOOSE LARGEST SAMPLE AND ! UPDATE LIMS WG42409-104 SAR SAR 20uL_]7_C023A:SUR/....... & MS' jlSDuL FC039B-AUT7 2-UL FC022A-SUR/ :50 uL FCC-09A-REC/ _____ F V :000 uL FSA-004 Rev 16, 16-Feb-2010 Page 2 Pagfc30 of 234 www.axysanalytical.com AXYS Analytical Services Ltd Sample Labeling Information: Batch ID: W G 42409 Method: m la-043 rb.03 Sample ID Sample Type and Description Date: If? _____ Analyst: 'i ~ftx Contract #:_____ 4731 Original Labelling LIMS label correct as per client L19064-1 L19064-2 L19064-3 L19064-4 L19064-5 L19064-6 L 19064-7 L19064-8 L19064-9 L19064-10 L19064-11 ftA ft M irvia ('Vi' T f _ CYrgM fn o t$ b TO .... O TH s n > f t K<<AA * UQ ^ vipu m o t t t Vpt-'-/-srls"----../.n..o...l.Xf......... ....--""--------- -- Pirtk ( n o n i; I 'ff5 4 u ^ O f&u m o t f t 17-- V----------- ^ m o e x'sf- e_ Ts a rw m o r s f fo iM P L . fcsvu e ftS^ogS y f a I o J o aaZ, O fty tA A l ' Z.OO -T-iw i I - A - t l t f S f p S & L-Jj H S Mi p o t f r L IM I f a r y f a lo O ia M iZ n rk ^ -J o o -m S 'fi^ i M f or>& 4 k in s O rr^ c d - Z o O 'W ^ 't- fr g i f - ci<3 LlKS O t t u s i T '' ~-A -2 o i f f o t j n f f o i% L in s X p l^iS ^ Z a o -lW W '-A -lS S F o g 2 2o0 a \ * t t 2 - % P f - i S o I/H S x p P le if J2oi?-i4'[(f 2 .-A -4 > i p p 75Zf- i f f isk > n ^ f'A U r 2 r ppi ^ ZHs fH (V \< P |!^ 2<x> *** m f/?f TrMi H h% rr'w e J : 'Z a - o - i^ S S - h - 'L M iPiio HS flA fy O rt'^'A ^-. Z O O - W t S b - A ' I ^ " k STF- 1124, LIKS f E 0 S' 0 ei fcr R" R L19064-12 L19064-13 y ft , Vd3 M.w iv-euM -- p ' aIa m oist- -H ssu e. t r ^ ' .f 5 r.srJIAJ tM/OiX-f i-fX w c e Jf L19064-14 L19064-15 L19064-16 tH I K/fPAJ "T ri W aV ^ o fs ir 'AotsV "tC'S'Cue I f f life___________________________________________ l x h s 2 o o -l4 -|g 5 " A -2 fl tffffti L IM S O r^'A al.' 8 "5 ' A - m i - f I tS'z i\A<,f oAA J ^ - n ^ - ^ vo;J<UA.ii lH5 CV-v^ a-aI'- x o o - \ L r t ^ ~ A rt& H STo H nsvohiL Uns On-WAcJit ZOO - - A -x R \V V S V oGH- ^ [7 t Ef f Oy m'Ar - 2.00 A " \ V X"V oA"2l ^ s/ FW O-305 Rev 07, 18-Dec-2012 Reviewed by date Pane \i a`A of Page"3T of 234 www.axysanalytical.com AXVS A naiytica! S ervices Ltd Sample Labeling Information: Batch ID: W G 42409 Method: MLA-043 R8.03 Date: _______ Analyst: S a m p le ID Sam ple Type and D escription O riginal Lab ellin g WG42409-101 (BLANK) WG42409-102 (SPM) WG42409-103 (DUP) (Jo ( U U r ) WG42409-104 (SAR) c( c a lo v l ^ K u u - k j f r f i c e d u n l U a 4 < . r u w A s t/% > v \ V V e l i m i tv t.6 > & & t \ Q i'K A 1 ^ M v ' vc {O .T - K e F i > 1 ^ 0 0 ^ ' i Lf ; Q ir& i- -------- 1---------------------V -- f -- - j f -- M -- ---- - --------- /i S : sny- D ^ 'aJL i -V -- ------ --- _ ...... .... A ss *>* 4 - i - \ FWO-305 Rev 07, 18-Dec-2012 Reviewed by_ date_ 1 \ 1 Contract #: NONE iO ~ e c fa i/Y > ? 6 (f- LIMS label correct as per dient EK .... .......... -- ----------------*---------- ------------ - X L - .................................... iC Page ' ^ of < Page 32 of 234 www.axysanalytical.com AXYS Analytical Services Ltd Sample Wt & Moistures: Batch ID: WG42409 Method: m l a -043 r s .03 Sam ple ID Tare (a) Date: I&JomI j Analyst: Balance or Syringe # Tare + W et Moisture (SLA-015) (all w eights in gram s) Tare + dry Tare (b) Tare + wet L19064-1 Tare + dry S am ple Wt or Vot (SLA-Q85) (g or m L) Backup (g) L19064-2 Z ~1f L19064-3 L19064-4 ''t-'/'P* <Z> L19064-5 L19064-6 L19064-7 "2-' 1 Z-2 OC? "V 2P L19064-8 2-VX L19064-9 L19064-10 2,2A f 2..0Z. -'v/ SO L19064-11 L19064-12 ~L-$ L19064-13 L19064-14 Z 'Z Z ~L- o b ^/U D L19064-15 L19064-16 WG42409-101 (BLANK) 2 'a T2. (iO\ ' O J -- W G42409-102 (SPM) M oistures Into oven; Date; E xtraction Inform ation: Drying; Dryinq Time: Extraction Solvent used: Beqin: Date: FWO-306 Rev 08, 02-Jan-2011 Time: Soxhlet: mins T im e : Analyst: Dean Stark: Analyst: Analyst: Out of Oven: Date: Steam Distillation: Volume; End: Date: Reviewed bv dale U l f V U W ............. T im e : Base Digest: Reaqent: mL T im e : 'l.fo Analyst;______ _ Voi: mL Analyst: 4 Paqe 'M K j of ' r --------P a g l ^ T o f 234 www.axysanalytical.com AXYS Analytical Services Ltd Sample Wt & Moistures: Batch ID: WG42409 Date: <f f if fu l\ Analyst: Balance or Syringe # % Method: m l a -043 R8.03 Sam ple ID Tare (a) Tare + W et Moisture (SLA-015) (all w eights in gram s) Tare + dry Tare (b) T are + wet Tare + dry W G42409-103 (DUP) Wa2408-43-(BP)-- ' W G42409-104 (SAR) Sample Wt or Vol (SLA-085) (g or mL) Backup (g) Moistures into oven: Date: _ Extraction inform ation: Drying: Drying Time: _ Extraction Solvent used Begin: Date:_________ FWO-306 Rev 08, 02-Jan-2011 __ T im e : Soxhlet: mins Analyst: Dean Stark: Analyst: T im e : Analyst: Out of Oven: Date: Steam Distillation: V o lu m e :_____ End: Date: Reviewed by date \ V yy T im e: Base Digest: R e a g e n t:___ mL Time: Analyst:^ Vol; mL Analyst: Page IS^ 0 of a Page 34 of 234 www.axysanalytical.com Spiking Sheet: Batch ID: W G 4 2 4 0 9 AXYS Analytical Services Ltd M e th o d : M LA-043 R8.03 Sample ID L19064-1 L19064-2 L19064-3 L19064-4 L19064-5 L19064-6 L19064-7 L19064-8 L19064-9 L19064-10 L19064-11 L19064-12 L19064-13 L19064-14 L19064-15 L19064-16 WG42409-101 (BLANK) WG42409-102 (SPM) Sample ID lilt]*) 7 f o g .- .i o Z S ? fV ) - l e H Sfrt- Before extraction Sum. ID:}?^7rH4-,SOII/c>| Date: tA Voi.: pL Svrinqe#: Ampoule: 3A- Analvst: vta Before extraction Sum. ID: v i Svrinqe#: Ampoule: Analvst: UL Cleanup 3rd. Cleanup Std. Recovery Recovery Sum: fm iA S m ZSurr.: Surr.:'fCD&fAi'fWi(ol Surr.: Date: n'R3M.l5 4 vpi.: 20 uL !AASvrinqe#: Ampoule: Date: Voi.: Svrinae#: Ampoule: Date: tl uL Voi.: F.V.: Cf>o&__ pL uL Svrinqe#: SAi'-F Ampoule: t'f' Date: Voi.: F.V.: Svrinqe#: Ampoule: Start t im e ^ C 2- Start time: qf^tart time: Start time: Fin. time: Fin. time:-------^ time: Fin. time: Time Witness Time Witness Analyst: V-& Analyst: i1U x/ I1 2 > \\Z~b (fOC j \/ a z 'h t>*QU yf I7 3 - V 117.1- V lJb UQU )17P> &t>U v V i:? hL \/ lULs 1/ 1 $) (s V 1171 V IM V WzA V i ! ? A &$)u V (1?^ m i V [M V l l 3 o evis % Analvst: ^-fA V V/ sj Analyst: V \/ V ________V_____ V V V \/ \f V V ______ V______ V V b iZ I/02Date: IC J fr A j i3 Voi.:. Syringe#: ____ . HL Ampoule#: Analyst: Time W itness Date: f / 1/ f i r r - J Voi.:. ilrO _pL Syringe#: _ Ampoule#:^ o A n a ly s t: Time Witness Authentic Std: Date Voi Syringe# Ampoule# A n a ly s t: Time ML Witness .e o ^ pL uL . FW0-31 Rev 08, 16-Feb-2010 Reviewed by A 4"*^- date U ~ 3flU A ^4> m Page ^ o r P age 36''of 234 www.axysanalytical.com AXYS Analytical Services Ltd Spiking Sheet: Batch D: W G 4 2 4 0 9 ____________ Method: m l a -043 R8.03 SLA-017 Witness name/ initials: A S am ple ID Before extraction Sum ID: Date: to7TAAJ> Vol.: Up pL Svrinqe#: li *4 Ampoule: rf- Analyst: Before extraction Surr. ID: f C-cf2^A~S0n/o\ Date: loTTAAJ3 Vol.: pL Svrinqe#: #Vfc>"i-- Ampoule: S- Analyst: Cleanup Std. Surr.:FcoZ2A-^il^ Date: n Ga-vill Voi.: i-o uL Svrinqe#: Ampoule: p Start time^vST Fin. time: Time Witness fi WG42409-103 (DUP) WG42469=f63~(Pt3P)------- H 5 o it>L-- -------- -------- WG42409-104 (SAR) ---- Time ---- Ilio Witness -- -- Analyst: V -- ---- * sJ V i ,'p L -- Cleanup Std. Surr.: Date: Voi.: uL Svrinqe#: Ampoule: Start tim e :_____ Fin. time: Recovery Surr.: Date: 11 Vol.: e pL F.V.: 4oo'o pL Svrinqe#: Q\ itf Ampoule: 1^ Start time: H'o Fin. time: f2;5S Analyst: Analyst: V Recovery Surr.: Date: Voi.: F.V.: Svrinqe#: Ampoule: Start time: Fin. time: pL uL Analyst: ________ V_______ Sample ID Authentic Std: Date: Voi.: Svrinqe#: Ampoule#: Analvst: Time uL Witness Authentic Std: Date: Voi.: Syrinqe#: Ampoule#: Analvst: Time uL Witness Authentic Std Date ` Voi. : Syringe# Ampoule# Analyst Time PL Witness FWO-310 Rev 08, 16-Feb-2010 Reviewed date Page . ^ q 0 i -\ P age 36 o f 234 www.axysanalytical.com AXYS Analytical Services Ltd Extract Splitting Sheet: Batch ID: WG42409_______ Balance #: b ^ 1- 0 Method: mla-043 R8.03 Sample ID L19064-1 L19064-2 L19064-3 L19064-4 L19064-5 L19064-6 L19064-7 L19064-8 L19064-9 L19064-10 L19064-11 L19064-12 L19064-13 L19064-14 L19064-15 L19064-16 WG42409-101 (BLANK) WG42409-102 (SPM) WG42409-103 (DUR) WG47409-103 (DUR) C-tube (column a) C-iube + total extract (column b) Total Extract (b-a) 4 --^ 4 -' h _______ Portion + c-tube (column c) 6a \ Analyst: Weight (g) Portion: (c-a) Date: 1\ If iM W .Time: a Portion: //'T - (direct wt) 3-a * 3 -< $ > 1 > ' 0 i.R o Portion: (direct wt) Portion: (direct wt) 3* b o "2,4-^ ---- ,-- -- -- Portion: (direct wt) FWO-311 Rev 06, 1-Oct-2010 Reviewed by " 3 ^ Date Page t - '_ Pag_3 7 o f 234 www.axysanalytical.com A X Y S A n alytical S ervices Ltd LC Methods W orksheet Workgroup: W G 4 2 4 0 9 ________ Method: M LA -043 RB.03 S am ple P re p a ra tio n and E x tra c tio n : Analyst: ______ Date: H Jau!3 Fraction: R eagent Lo t# V o i mL T im e V o l. mL T im e V o l. mL T im e Extract pH check/adjust 5 0 % fo rm ic a cid 0 .0 5 M K O H in M e O H 3 % a c e tic acid 0.3% (N H aO H )/ 99% M eOH R e a g e n t w a te r type: J M - C a rbo n (50 mg ) a c e t o n it r ile ( C H aC N ) F o rm ic acid cone. NH^O H cone. EOTA 0 .1 4 M N a 2H P O i b u f fe r pH 2 HCI cone. a m m o n iu m a c e la te b u ffe r 1 M , pH 5,0 a m m o n iu m a c e ta te bu ffe r 1 M, pH 6.5 a m m o n iu m a c e ta te b u ffe r 1 M , p H 4.Q P h o sp h a te b u ffe r 1 M, pH 6.0 enzym e ft" g lu c o ro n id a s e /s u ifa ta s e enzym e ft- g iu c o ro n id a s e 5 % N H 4O H in w a te r H20 type 5% b o ra te bu ffe r pH 9 0.0 1 2 g /m L F M O C -C I A c id ifie d w a te r w ith HC I (pH ) 3:1 M lB E :M e O H S o d iu m a c e ta te b u ffe r 0 .5M , pH 4 10% a ce ta te b u ffe r pH 4 E D C .H C I 1 40m M K O H in 80:20 M eO H : W a te r M eOH 32 l o too oS't Shak^nTppejx Analyst: Date: / o j i t, Time: Firnes: I D erlvatization Analyst: Date: Start time: End time: 1- pH ^ ate: /I 2 a'v' ' ^ Time: f; ^ Analyst: 2. pH Date: Time: Analyst: 3. pH Date: Time: Analyst: M illip ore: Analyst: Date: Time: F iltratio n: Analyst: Date: Time: In c u b a tio n : Analyst: Date: Start time: End time: M anifold# A n a ly s t Sampie ID flfPl? Date: H&&***}> Position L19064-1 L19064-2 L19064-3 L19064-4 f/ 2. 1 L19064-5 L19064-6 L19064-7 L19064-8 L19064-9 L19064-10 j p[ \ 1 r fo If tX t L19064-11 L19064-12 L19064-13 L19064-14 L19064-15 L19064-16 ^ WG42409-101 (BLANK) WG42409-102 (SPM) WG42409-103 (DUP) WG42409-103 (DUP) WG42409-104 (SAR) 'C '' IL --X ,f fo \ il fX l ys I t #fiiter units FWO-333 Rev 04, 26-Oct-2011 Reviewed by: Date: P a g e 5 of ^ ^)][^ Page 38 of 234 www.axysanalytical.com AXYS Analytical Services Ltd LC Methods Cleanup Worksheet W o rk g ro u p : WG42409____ M e th o d : m l a -043 R8.03 F ra c tio n A nalyst: S P E P r e - E lu t io n / C o n d it io n ir ig: Date: / Analyst: SPE type: t^A)c Lot#: 0 14 -7 - Reagent Lot Voi. Time # mL 0.1 M Formic acid 0.3% NH4OH/ 99% MeOH 1: MeOH I M r of1^ 0a 2: MeOH Reagent water type: ... _.... Acidified water H?0 Type (with HCI) pH DCM 2:30:68 Formic acid: Acetomtriie:MtBE 2% NH4 OH in water HjO Type Hexane 1: Acetonitrife (CH3 CN) 2: Acetonitrile (C H 3 CN) 0.1 M HCI/ 5% MeOH W ash: Load Sam ple: ^ E lu tio n : Reagent Reagent water type: 50% 0,1 M Formic acid/ 50% MeOH 0.1 M Formic add 0.3% basic (NH4OH)/ 99% CS water 2 0 % MeOH/ 80% 0.1 M Formic acid Lot # /S ty sr Voi. Time mL X /2 :*p iX-lS I2 -^ 31* " ( J u Acidified water H?OTvpe (with HCi) pH 0.1 M HCi/ 5% MeOH Acetonitrile (CH3CN) 5% MeOH/ 95% water H20 Type 2% NH4OH in water. H20 Type Hexane Reagent MeOH 0.3% NH4OH/ 99% MeOH Carbon (optional) 1:1 Acetone:MeOH 2 % Formic acid in MeOH Lot Voi. Time # mL 4 -.o o Acetonitrile (CH3CN) Hexane 2:30:68 Formic acid: Acetonitrile: MtBE DCM 10:20:70 NH4OH: MeOHtMtBE 1:1 DCM:MeOH 3:1 MtBE: MeOH Ethyl acetate R e c o n s titu te fo r jjv ia l: Date: Analyst: Reagent 0.1% Formic acid buffer 1: MeOH 2 : MeOH 3: MeOH 0.3% NH4OH/ 99% MeOH 70:30 MeOH: water HjO Type Lot Voi. Tim e # mL / / / / / / / 1:1 MeOH: 0.1% / acetate buffer / 1:1 C H 3 C N : / Ammonium acetate, Acetic acid buffer 1:1 0.1% Acetic/acid in CHaCN:Water HsO Type / 1:1 0.2% Acetic acid in CH3CN:w4ter H20 Type / Reagent wter type :___ I MtBE 10:20:70 NH4OH: MeOH: MtBE 0.5M Sodium Acetate Buffer pH 4 1:1 Acetonitrile: Acetone MtBE DCM 5% Acetonitrile in water H20 Type MeOH 9:1 MtBE: MeOH 1:1 Acetonitrile: Acetone 2 % Formic acid in MtBE / j / !/ / _________ / MeOH= Methanol; MtBE= Methyl-l-butyi ether; DCM= Dichloromethane; MeCN= CH3CN= Acetonitrile; NH4 OH= Ammonium hydroxide (30% aqueous unless otherwise spec.); CS= Canadian Springs FWO-334 Rev 03, 18-Sep-2012 Reviewed by: Date: Page G of ^ j ) ] Page 39 of 234 www.axysanalytical.com Notes: B a tc h ID ; WG42409 M e th o d : MLA-043 R8.03 AXYS Analytical Services Ltd D ate: A n a ly s t Fraction N otes: Reviewed by _____ _ date FWO-314 Rev 04, 13-May-2008 Page. ^ o f __ P ag F 40 of 234 www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF088 Sample Collection: 09-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-1 Matrix: TISSUE Sample Size: 2.05 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 18:09:13 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 21 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.488 (L) U 0.488 (L) U 0.488 (L) U 0.488 (L) U 0.488 (L) U 0.488 (L) 0.540 0.488 (L) 0.721 0.488 (L) U 0.488 (L) U 0.976 (L) U 0.976 (L) 56.6 0.976 (L) U 0.585 (L) 7:59 8:35 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L 19064- 1_Form1 A_FC3G_013S21_SJ1554629.html; Workgroup: W G42409;DesignID: 1980 ] Page 41 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-1_Form1A_FC3G_013S21_SJ1554629.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF088 Sample Collection: 09-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-1 Matrix: TISSUE Sample Size: 2.05 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 18:09:13 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 21 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 24.0 27.5 80.0 28.5 23.2 19.9 15.4 31.0 35.8 99.9 114 111 119 96.6 83.0 64.3 86.2 99.3 5:14 6:21 7:06 7:29 7:59 9:11 9:58 8:21 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 15.0 125 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-1_Form2_FC3G_013S21_SJ1554629.html; Workgroup: WG42409; Design ID: 1980 ] Page 42 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-1_Form2_FC3G_013S21_SJ1554629.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF093 Sample Collection: 15-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-2 Matrix: TISSUE Sample Size: 2.11 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 18:26:14 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 22 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.474 (L) U 0.474 (L) U 0.474 (L) U 0.474 (L) U 0.474 (L) U 0.474 (L) 1.67 0.474 (L) 1.26 0.474 (L) 2.72 0.474 (L) U 0.948 (L) U 0.948 (L) 432 0.948 (L) 4.07 0.569 (L) 7:59 8:35 9:11 8:21 9:59 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-2_Form1A_FC3G_013S22_SJ1554630.html; Workgroup: W G42409;DesignID: 1980 ] Page 43 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-2_Form1A_FC3G_013S22_SJ1554630.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF093 Sample Collection: 15-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-2 Matrix: TISSUE Sample Size: 2.11 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 18:26:14 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 22 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 23.9 28.2 76.5 27.4 21.5 21.5 16.8 27.0 33.5 99.4 118 106 114 89.8 89.5 70.2 75.0 93.1 5:14 6:22 7:06 7:29 7:59 9:11 9:58 8:21 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 15.6 130 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-2_Form2_FC3G_013S22_SJ1554630.html; Workgroup: WG42409; Design ID: 1980 ] Page 44 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-2_Form2_FC3G_013S22_SJ1554630.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF008 Sample Collection: 03-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-3 Matrix: TISSUE Sample Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 18:43:07 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 23 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) 0.577 0.495 (L) 0.581 0.495 (L) U 0.495 (L) U 0.990 (L) U 0.990 (L) 186 0.990 (L) 2.25 0.594 (L) 7:59 8:33 8:17 9:59 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-3_Form1A_FC3G_013S23_SJ1554631.html; Workgroup: W G42409;DesignID: 1980 ] Page 45 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-3_Form1A_FC3G_013S23_SJ1554631.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF008 Sample Collection: 03-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-3 Matrix: TISSUE Sample Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 18:43:07 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 23 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 26.5 28.9 73.8 27.8 23.2 23.3 15.6 29.0 36.9 110 120 102 116 96.8 97.0 64.8 80.7 103 5:11 6:20 7:06 7:29 7:59 9:09 9:58 8:17 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 14.9 124 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-3_Form2_FC3G_013S23_SJ1554631.html; Workgroup: WG42409; Design ID: 1980 ] Page 46 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-3_Form2_FC3G_013S23_SJ1554631.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF019 Sample Collection: 03-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-4 Matrix: TISSUE Sample Size: 2.07 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 19:00:08 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 24 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.483 (L) U 0.483 (L) U 0.483 (L) U 0.483 (L) U 0.483 (L) U 0.483 (L) 0.590 0.483 (L) 0.648 0.483 (L) U 0.483 (L) U 0.966 (L) U 0.966 (L) 10.2 0.966 (L) 0.846 0.580 (L) 7:59 8:33 8:17 9:58 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-4_Form1A_FC3G_013S24_SJ1554632.html; Workgroup: W G42409;DesignID: 1980 ] Page 47 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-4_Form1A_FC3G_013S24_SJ1554632.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF019 Sample Collection: 03-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-4 Matrix: TISSUE Sample Size: 2.07 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 19:00:08 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 24 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 13.7 28.3 73.6 26.5 23.2 19.3 10.4 34.4 35.3 57.3 118 102 111 96.6 80.3 43.5 95.5 98.2 5:15 6:21 7:06 7:28 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFOA 12.0 14.2 118 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-4_Form2_FC3G_013S24_SJ1554632.html; Workgroup: WG42409; Design ID: 1980 ] Page 48 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-4_Form2_FC3G_013S24_SJ1554632.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF032 Sample Collection: 03-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-5 Matrix: TISSUE Sample Size: 2.13 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 19:17:09 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 25 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.469 (L) U 0.469 (L) U 0.469 (L) U 0.469 (L) U 0.469 (L) U 0.469 (L) 1.28 0.469 (L) 1.23 0.469 (L) 0.649 0.469 (L) U 0.939 (L) U 0.939 (L) 56.1 0.939 (L) U 0.563 (L) 8:03 8:35 9:11 8:21 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-5_Form1A_FC3G_013S25_SJ1554633.html; Workgroup: W G42409;DesignID: 1980 ] Page 49 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-5_Form1A_FC3G_013S25_SJ1554633.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF032 Sample Collection: 03-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-5 Matrix: TISSUE Sample Size: 2.13 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 19:17:09 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 25 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 29.5 32.3 82.0 28.0 22.4 24.2 17.6 35.5 35.0 123 135 114 117 93.4 101 73.2 98.5 97.2 5:12 6:22 7:06 7:29 7:59 9:11 9:58 8:21 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 15.2 127 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-5_Form2_FC3G_013S25_SJ1554633.html; Workgroup: WG42409; Design ID: 1980 ] Page 50 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-5_Form2_FC3G_013S25_SJ1554633.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF150 Sample Collection: 21-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-6 Matrix: TISSUE Sample Size: 2.21 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 19:34:02 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 26 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.452 (L) U 0.452 (L) U 0.452 (L) U 0.452 (L) U 0.452 (L) U 0.452 (L) 0.984 0.452 (L) 0.955 0.452 (L) 0.559 0.452 (L) U 0.905 (L) U 0.905 (L) 57.8 0.905 (L) 3.87 0.543 (L) 7:59 8:35 9:11 8:17 9:59 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-6_Form1A_FC3G_013S26_SJ1554634.html; Workgroup: W G42409;DesignID: 1980 ] Page 51 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-6_Form1A_FC3G_013S26_SJ1554634.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF150 Sample Collection: 21-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-6 Matrix: TISSUE Sample Size: 2.21 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 19:34:02 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 26 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 22.2 26.7 73.6 24.8 22.2 20.4 11.9 35.8 34.9 92.3 111 102 103 92.4 85.1 49.7 99.5 96.9 5:16 6:20 7:06 7:29 7:59 9:11 9:59 8:17 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 15.1 126 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-6_Form2_FC3G_013S26_SJ1554634.html; Workgroup: WG42409; Design ID: 1980 ] Page 52 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-6_Form2_FC3G_013S26_SJ1554634.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF154 Sample Collection: 21-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-7 Matrix: TISSUE Sample Size: 1.99 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 19:51:03 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 27 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.503 (L) U 0.503 (L) U 0.503 (L) U 0.503 (L) U 0.503 (L) U 0.503 (L) 0.701 0.503 (L) 0.609 0.503 (L) U 0.503 (L) U 1.01 (L) U 1.01 (L) 14.5 1.01 (L) U 0.603 (L) 7:59 8:33 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-7_Form1A_FC3G_013S27_SJ1554635.html; Workgroup: W G42409;DesignID: 1980 ] Page 53 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-7_Form1A_FC3G_013S27_SJ1554635.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF154 Sample Collection: 21-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-7 Matrix: TISSUE Sample Size: 1.99 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 19:51:03 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 27 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 26.8 27.5 73.0 25.8 22.0 13.8 10.4 33.8 34.3 112 115 101 107 91.7 57.3 43.5 93.9 95.2 5:12 6:21 7:06 7:29 7:59 9:09 9:58 8:17 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 15.0 125 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-7_Form2_FC3G_013S27_SJ1554635.html; Workgroup: WG42409; Design ID: 1980 ] Page 54 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-7_Form2_FC3G_013S27_SJ1554635.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF156 Sample Collection: 21-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-8 Matrix: TISSUE Sample Size: 2.12 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 20:07:56 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 28 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.943 (L) U 0.943 (L) 12.3 0.943 (L) 1.74 0.566 (L) 8:17 9:56 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-8_Form1A_FC3G_013S28_SJ1554636.html; Workgroup: W G42409;DesignID: 1980 ] Page 55 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-8_Form1A_FC3G_013S28_SJ1554636.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF156 Sample Collection: 21-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-8 Matrix: TISSUE Sample Size: 2.12 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 20:07:56 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 28 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 19.4 26.8 75.4 26.6 24.5 17.6 11.1 34.3 36.5 80.9 112 105 111 102 73.3 46.1 95.2 102 5:12 6:21 7:06 7:29 7:59 9:09 9:56 8:17 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 14.6 122 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-8_Form2_FC3G_013S28_SJ1554636.html; Workgroup: WG42409; Design ID: 1980 ] Page 56 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-8_Form2_FC3G_013S28_SJ1554636.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF171 Sample Collection: 29-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-9 Matrix: TISSUE Sample Size: 2.24 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 20:24:57 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 29 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.446 (L) U 0.446 (L) U 0.446 (L) U 0.446 (L) U 0.446 (L) U 0.446 (L) 0.905 0.446 (L) 0.942 0.446 (L) 0.600 0.446 (L) 2.04 0.893 (L) U 0.893 (L) 345 0.893 (L) 55.4 0.536 (L) 8:03 8:35 9:11 6:27 8:21 9:59 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-9_Form1A_FC3G_013S29_SJ1554637.html; Workgroup: W G42409;DesignID: 1980 ] Page 57 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-9_Form1A_FC3G_013S29_SJ1554637.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF171 Sample Collection: 29-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-9 Matrix: TISSUE Sample Size: 2.24 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 20:24:57 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 29 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 30.4 31.9 73.5 22.4 22.2 20.7 15.2 28.5 35.8 127 133 102 93.2 92.6 86.4 63.2 79.1 99.5 5:12 6:21 7:06 7:29 7:59 9:11 9:59 8:21 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 14.7 123 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-9_Form2_FC3G_013S29_SJ1554637.html; Workgroup: WG42409; Design ID: 1980 ] Page 58 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-9_Form2_FC3G_013S29_SJ1554637.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF110 Sample Collection: 15-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-10 Matrix: TISSUE Sample Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 20:41:50 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 30 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) 0.839 0.495 (L) 0.820 0.495 (L) U 0.495 (L) U 0.990 (L) U 0.990 (L) 22.0 0.990 (L) U 0.594 (L) 7:59 8:33 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-10_Form1A_FC3G_013S30_SJ1554638.html; Workgroup: WG42409; Design ID: 1980 ] Page 59 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-10_Form1A_FC3G_013S30_SJ1554638.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF110 Sample Collection: 15-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-10 Matrix: TISSUE Sample Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 20:41:50 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 30 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 31.1 34.3 78.7 28.7 25.1 25.9 18.7 37.1 36.0 129 143 109 119 104 108 78.0 103 100 5:10 6:21 7:06 7:29 7:59 9:09 9:58 8:17 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 14.2 119 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-10_Form2_FC3G_013S30_SJ1554638.html; Workgroup: WG42409; Design ID: 1980 ] Page 60 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-10_Form2_FC3G_013S30_SJ1554638.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF126 Sample Collection: 16-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-11 Matrix: TISSUE Sample Size: 2.08 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 20:58:51 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 31 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) 0.895 0.481 (L) 0.710 0.481 (L) U 0.481 (L) U 0.962 (L) U 0.962 (L) 43.9 0.962 (L) U 0.577 (L) 7:59 8:33 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-11_Form1A_FC3G_013S31_SJ1554639.html; Workgroup: WG42409; Design ID: 1980 ] Page 61 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-11_Form1A_FC3G_013S31_SJ1554639.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF126 Sample Collection: 16-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-11 Matrix: TISSUE Sample Size: 2.08 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 20:58:51 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 31 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS V 24.0 43.7 182 V 24.0 37.8 157 72.0 81.0 112 24.0 31.0 129 24.0 27.6 115 24.0 24.4 102 24.0 19.6 81.8 36.0 41.3 115 36.0 41.5 115 5:10 6:20 7:06 7:28 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFOA 12.0 15.2 127 7:06 (1 ) W here applicable, custom lab flags have been used on this report; V = surrogate recovery is not within m ethod/contract control limits. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-11_Form2_FC3G_013S31_SJ1554639.html; Workgroup: WG42409; Design ID: 1980 ] Page 62 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-11_Form2_FC3G_013S31_SJ1554639.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF136 Sample Collection: 21-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-12 Matrix: TISSUE Sample Size: 2.08 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 21:15:52 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 32 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.962 (L) U 0.962 (L) 4.12 0.962 (L) U 0.577 (L) 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-12_Form1A_FC3G_013S32_SJ1554640.html; Workgroup: WG42409; Design ID: 1980 ] Page 63 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-12_Form1A_FC3G_013S32_SJ1554640.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF136 Sample Collection: 21-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-12 Matrix: TISSUE Sample Size: 2.08 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 21:15:52 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 32 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 31.0 32.0 79.9 26.9 25.9 20.1 11.8 41.3 36.2 129 133 111 112 108 83.7 49.2 115 101 5:12 6:20 7:06 7:28 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFOA 12.0 15.6 130 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-12_Form2_FC3G_013S32_SJ1554640.html; Workgroup: WG42409; Design ID: 1980 ] Page 64 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-12_Form2_FC3G_013S32_SJ1554640.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF182 Sample Collection: 13-Dec-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-13 Matrix: TISSUE Sample Size: 2.22 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 21:32:45 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 33 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.901 (L) U 0.901 (L) 5.72 0.901 (L) U 0.541 (L) 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-13_Form1A_FC3G_013S33_SJ1554641.html; Workgroup: WG42409; Design ID: 1980 ] Page 65 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-13_Form1A_FC3G_013S33_SJ1554641.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF182 Sample Collection: 13-Dec-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-13 Matrix: TISSUE Sample Size: 2.22 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 21:32:45 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 33 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 33.0 29.5 74.2 24.7 23.0 24.2 16.2 31.0 35.3 137 123 103 103 95.9 101 67.4 86.1 98.2 5:11 6:22 7:06 7:29 7:59 9:09 9:59 8:17 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 16.1 134 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-13_Form2_FC3G_013S33_SJ1554641.html; Workgroup: WG42409; Design ID: 1980 ] Page 66 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-13_Form2_FC3G_013S33_SJ1554641.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF054 Sample Collection: 07-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-14 (A) Matrix: TISSUE Sample Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 21:49:46 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 34 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) 1.08 0.495 (L) 1.31 0.495 (L) 0.773 0.495 (L) U 0.990 (L) U 0.990 (L) 91.7 0.990 (L) 1.52 0.594 (L) 7:59 8:32 9:09 8:17 9:58 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-14_Form1A_FC3G_013S34_SJ1554642.html; Workgroup: WG42409; Design ID: 1980 ] Page 67 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-14_Form1A_FC3G_013S34_SJ1554642.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF054 Sample Collection: 07-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-14 (A) Matrix: TISSUE Sample Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 21:49:46 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 34 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS V 24.0 36.9 154 24.0 35.2 147 72.0 76.8 107 24.0 29.1 121 24.0 24.5 102 24.0 27.9 116 24.0 20.4 84.9 36.0 37.0 103 36.0 37.2 103 5:12 6:20 7:06 7:29 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFOA 12.0 16.2 135 7:06 (1 ) W here applicable, custom lab flags have been used on this report; V = surrogate recovery is not within m ethod/contract control limits. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-14_Form2_FC3G_013S34_SJ1554642.html; Workgroup: WG42409; Design ID: 1980 ] Page 68 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-14_Form2_FC3G_013S34_SJ1554642.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF054 (Duplicate) Sample Collection: 07-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING W G42409-103 (DUP L19064-14) Matrix: TISSUE Sample Size: 2.22 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 22:06:47 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 35 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) 1.18 0.450 (L) 1.25 0.450 (L) 0.896 0.450 (L) U 0.901 (L) U 0.901 (L) 103 0.901 (L) 1.69 0.541 (L) 7:59 8:32 9:07 8:17 9:58 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-103_Form1A_FC3G_013S35_SJ1554643.html; Workgroup: WG42409; Design ID: 1980 ] PPaaggee 69 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_WG42409-103_Form1A_FC3G_013S35_SJ1554643.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF054 (Duplicate) Sample Collection: 07-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING W G42409-103 (DUP L19064-14) Matrix: TISSUE Sample Size: 2.22 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 22:06:47 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 35 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G 013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 32.7 136 V 24.0 36.1 150 72.0 77.9 108 24.0 27.7 116 24.0 24.8 103 24.0 25.5 106 24.0 20.6 86.0 36.0 31.2 86.7 36.0 41.3 115 5:10 6:21 7:06 7:29 7:59 9:07 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFOA 12.0 14.4 120 7:06 (1) W here applicable, custom lab flags have been used on this report; V = surrogate recovery is not within m ethod/contract control limits. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-103_Form2_FC3G_013S35_SJ1554643.html; Workgroup: WG42409; Design ID: 1980 ] Page 70 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_WG42409-103_Form2_FC3G_013S35_SJ1554643.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 PERFLUORINATED ORGANICS AN ALYSIS REPO RT RELATIVE PERCEN T DIFFERENCE AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Client ID: MSF054 Concentration Units: FALL 2012 TENNESSEE RIVER SAMPLING ng/g (wet weight basis) COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA L19064-14 (A) LAB FLA G 1 CONC. FOUND U U U U U U 1.08 1.31 0.773 U U 91.7 1.52 WG42409-103 LAB FLAG 1 CONC. FOUND U U U U U U 1.18 1.25 0.896 U U 103 1.69 MEAN R E LA T IV E PERCENT D IF F E R E N C E 1.13 1.28 0.835 97.3 1.61 8.29 4.95 14.7 11.4 10.2 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ These pages are part of a larger report that may contain inform ation necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: RPD.xsl; Created: 31-Jan-2013 11:32:34; Application: XMLTransformer-1.13.30; ReportFilename: RPD_FC_LC_PFOA-RPD_WG42409-103_L19064-14_.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - RPD_FC_LC_PFOA-RPD_WG42409-103_L19064-14_.html) Page 71 of 234 www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF064 Sample Collection: 07-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-15 Matrix: TISSUE Sample Size: 2.51 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 22:23:48 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 36 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.398 (L) U 0.398 (L) U 0.398 (L) U 0.398 (L) U 0.398 (L) U 0.398 (L) 1.38 0.398 (L) 0.606 0.398 (L) 0.700 0.398 (L) U 0.797 (L) U 0.797 (L) 99.8 0.797 (L) 1.46 0.478 (L) 7:59 8:32 9:07 8:17 9:59 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-15_Form1A_FC3G_013S36_SJ1554644.html; Workgroup: WG42409; Design ID: 1980 ] Page 72 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-15_Form1A_FC3G_013S36_SJ1554644.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. MSF064 Sample Collection: 07-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-15 Matrix: TISSUE Sample Size: 2.51 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 22:23:48 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 36 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 27.6 31.6 80.1 27.6 21.6 9.61 8.36 21.7 35.4 115 132 111 115 90.2 40.1 34.8 60.4 98.5 5:10 6:20 7:06 7:28 7:59 9:07 9:59 8:17 7:17 CLEANUP STANDARD 13C8-PFOA 12.0 15.6 130 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-15_Form2_FC3G_013S36_SJ1554644.html; Workgroup: WG42409; Design ID: 1980 ] Page 73 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-15_Form2_FC3G_013S36_SJ1554644.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF072 Sample Collection: 09-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-16 Matrix: TISSUE Sample Size: 2.04 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 22:40:41 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 37 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.980 (L) U 0.980 (L) 1.66 0.980 (L) U 0.588 (L) 8:17 (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-16_Form1A_FC3G_013S37_SJ1554645.html; Workgroup: WG42409; Design ID: 1980 ] Page 74 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-16_Form1A_FC3G_013S37_SJ1554645.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. Contract No.: 4731 Lab Sam ple I.D.: C LIE N T SA M PLE NO. IPF072 Sample Collection: 09-Nov-2012 FALL 2012 TENNESSEE RIVER SAMPLING L19064-16 Matrix: TISSUE Sample Size: 2.04 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 22:40:41 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 37 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 28.6 29.7 74.6 26.3 20.8 20.8 12.8 34.2 37.7 119 124 104 109 86.7 86.5 53.5 95.0 105 5:11 6:21 7:06 7:28 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFOA 12.0 14.3 119 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-16_Form2_FC3G_013S37_SJ1554645.html; Workgroup: WG42409; Design ID: 1980 ] Page 75 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-16_Form2_FC3G_013S37_SJ1554645.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 1A PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Project No. Lab Sam ple I.D.: C LIE N T SA M PLE NO. Lab Blank Sample Collection: N/A N/A WG42409-101 Matrix: AQUEOUS (TISSUE METHOD) Sample Size: 2.00 g Sam ple Receipt Date: N/A Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 17:52:20 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 20 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G_013 S: 14 Concentration Units: ng/g This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND R E P O R T IN G LIMIT (RL)2 R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U U U U U U U U U U U U U 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 1.00 (L) 1.00 (L) 1.00 (L) 0.600 (L) (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-101_Form1A_FC3G_013S20_SJ1554627.html; Workgroup: WG42409; Design ID: 1980 ] PPaaggee 76 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_WG42409-101_Form1A_FC3G_013S20_SJ1554627.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 2 PERFLUORINATED ORGANICS AN ALYSIS REPO RT AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Project No. Lab Sam ple I.D.: C LIE N T SA M PLE NO. LSaabmBpllaenCk ollection: N/A N/A WG42409-101 Matrix: AQUEOUS (TISSUE METHOD) Sample Size: 2.00 g Sam ple Receipt Date: N/A Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 17:52:20 Colum n ID: C18MS Extract Volume (uL): 4000 Sam ple Data Filename: FC3G_013 S: 20 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G 013 S: 14 Concentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LA BELED COMPOUND LAB FLA G 1 S P IK E CONC. CONC. FOUND R(%) 2 RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS V 24.0 38.7 161 24.0 35.7 149 72.0 81.4 113 24.0 26.1 109 24.0 18.7 78.1 24.0 18.2 75.7 24.0 20.8 86.5 36.0 42.3 117 36.0 42.7 119 5:16 6:21 7:06 7:29 8:03 9:11 9:59 8:21 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 13.0 109 7:06 (1) W here applicable, custom lab flags have been used on this report; V = surrogate recovery is not within m ethod/contract control limits. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-101_Form2_FC3G_013S20_SJ1554627.html; Workgroup: WG42409; Design ID: 1980 ] Page 77 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_WG42409-101_Form2_FC3G_013S20_SJ1554627.html) www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 8A PERFLU O RIN A TED O RG AN ICS ONGOING PRECISIO N AND R EC O V ER Y (OPR) AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Lab Sam ple I.D.: W G 42409-102 Matrix: TISSUE Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 17:01:25 Colum n ID: C18MS Extract Volume (uL): 4000 O PR Data Filename: FC3G_013 S: 17 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G 013 S: 14 A L L C O N C EN T R A TIO N S R E P O R T E D ON TH IS FORM A R E C O N C EN T R A TIO N S IN E X T R A C T , B A S E D O N A 1 m L E X T R A C T VOLUM E. COMPOUND LAB FLA G 1 SPIKE CONC. (ng/mL) CONC. FOUND (ng/mL) % RECOVERY R ET EN T IO N TIME PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 80.0 80.0 80.0 40.0 34.8 31.4 36.0 31.6 34.8 31.1 34.0 33.0 39.9 78.5 74.5 65.6 34.8 87.0 78.5 90.0 79.1 86.9 77.7 85.0 82.6 99.8 98.1 93.1 82.0 86.9 5:14 5:58 6:22 6:44 7:09 7:33 8:03 8:35 9:13 6:29 7:20 8:21 9:59 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ These pages are part o f a larger report that may contain inform ation necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: FC-Form8A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-102_Form8A_SJ1554623.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_WG42409-102_Form8A_SJ1554623.html) Page 78 of 234 www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 8B PERFLUORINATED ORGANICS ONGOING PRECISION AND RECOVERY (OPR) AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Contract No.: 4731 Lab Sam ple I.D.: W G 42409-102 Matrix: TISSUE Initial Calibration Date: 1 1-Ja n -2 0 13 Extraction Date: 10-Jan-2013 Instrument ID: LC MS/MS A nalysis Date: 11-Jan-2013 Time: 17:01:25 Colum n ID: C18MS Extract Volume (uL): 4000 O PR Data Filename: FC3G_013 S: 17 Injection Volume (uL): 15 Blank Data Filename: FC3G_013 S: 20 Dilution Factor: N/A Cal. Ver. Data Filename: FC3G 013 S: 14 A L L C O N C EN T R A TIO N S R E P O R T E D ON TH IS FORM A R E C O N C EN T R A TIO N S IN E X T R A C T , B A S E D O N A 1 m L E X T R A C T VOLUM E. LABELED COMPOUND LAB FLAG 1 SPIKE CONC. (ng/mL) CONC. FOUND (ng/mL) % RECOVERY RETENTION TIME 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 29.4 30.4 88.1 31.9 26.2 23.9 26.0 44.2 39.7 122 126 122 133 109 99.4 108 123 110 5:11 6:22 7:06 7:33 8:03 9:13 9:59 8:21 7:20 CLEANUP STANDARD 13C8-PFOA 12.0 15.1 126 7:06 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Kristina Coleman______ These pages are part o f a larger report that may contain inform ation necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: FC-Form8B.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-102_Form8B_SJ1554623.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_WG42409-102_Form8B_SJ1554623.html) Page 79 of 234 www.axysanalytical.com __________________________ I Sample List Report F fo T u ^ a - t c . __________________________________________________________ MassLynx 4.1 SCN627 Sample List: G:\Masslynx\lnst_G\_Master.PRO\SampleDB\FC3G_013.SPL Last Modified: Friday, January 11,2013 13:01:07 Pacific Standard Time Printed: Friday, January 11,2013 13:05:15 Pacific Standard Time Page 1 of 1 Page Position (1,1) File Name Sample ID File Text Bottle Signln SignOut Inj VoL 1 FC3G 013S001 M eO H,, 1 ,, 15uL L IU 1:8,A - - iM - 15.0 2 FC3G 013S002 M eOH,, 1 ,, 15uL 1:8,A - - 15.0 3 FC 3G 013S003 F C -L IN -4 2 1 5 1 T -A J /2 1 ,,15uL 2:1,A - - 15.0 4 FG 3G 013S004 FC -LIN -42151T -A ,,1/2 1 ,,15uL 5 FC3G 013S005 FC -LIN -42151T -B ,,1/2 1,,1 5uL 2:1,A - - 2:1,B - - . --- 15.0 15.0 6 FC 3G 013S006 F C -LIN -42151T -C ,,1/2 1,,15uL 7 FC 3G 013S 007 F C -LIN -42151T -D ,,1/2 1 ,,15uL 2:1,C - - 2:1,D - - --- - 15.0 15.0 8 F C 3 G 0 1 3 S 0 0 8 F C -L IN -4 2 1 5 1 T -E ,,1/2 1 ,,1 5 uL 2:1,E - - - 15.0 9 F C 3G 0 13S 009 F C -L IN -4 2 1 51T -F ,, 1/2 1 ,,1 5 uL 2:1,F - - - 15.0 10 F C 3G 01 3 S 0 1 0 F C -L IN -4 2 1 5 1 T -G ,,1/2 1,, 15uL 11 F C 3 G 0 1 3 S 0 1 1 F C -L IN -4 2 1 5 1 T -H ,,1 /2 1 ,,1 5 u L 2:2,A - - 2:2,B - - -- 15.0 15.0 J 12 FC3G 013S012 M eO H ,, 1,,15uL 1:8,A - -- 15.0 13 F C 3G 013S 013 M e O H ,, 1,,15uL 14 F C 3G 013S 014 F C -L IN -4 21 5 1 T -D ,,1 /2 1 ,,1 5 u L 15 F C 3G 01 3 S 0 1 5 F C -L IN -4 2 1 5 1 T -D ,,1 /2 1,, 15uL 1:8,A - -/ 15.0 2:1 ,D 2 :1 ,D - "t / 15.0 -1 15.0 I 16 F C 3G 013S 016 W G 4 2 4 0 9 -1 0 4 ,,S A R 1 ,W G 42409,15/4000uL 3:4,A - 15.0 17 FC 3G 013S 017 W G 4 2 4 09 -1 0 2 ,,S P M 1 ,W G 42409,15/4000uL 3 :4 ,B ' - - 15.0 18 FC 3G 013S018 M eO H ,, 1,, 15uL 1:8,A - - 15.0 19 F C 3G 013S 019 M e O H ,, 1 ,,15uL ' 1:8,A - - 15.0 20 FC 3G 013S020 W G 42409-101 ,,BLK 1,W G 42409,15/4000uL 3 :4,C - -- 15.0 21 F C 3 G 0 1 3 S 0 2 1 L 1 9 0 6 4 -1 ,, 1,W G 42409,15/4000uL 3 :4,D - - 15.0 22 FC3G 013S022 L19064-2,,. 1 ,W G 42409,15/4000uL 3 :4,E - -- 15.0 23 FC 3G 013S023 L19064-3,, 1 ,W G 42409,15/4000uL 3 :4 ,F - - -- 15.0 24 FC 3G 013S024 L19064-4 ,, 1 ,W G 42409,15/4000uL 3:5,A - - -- 15.0 25 FC 3G 013S025 L19064-5,, 1 ,W G 42409,15/4000uL 3 :5 ,B - - 15.0 26 FC 3G 013S026 L19064-6,, 1 ,W G 4 2 4 0 9 ,1 5 /4 0 0 0 u L .3:5, C - 15.0 27 F C 3G 013S 027 L19064-7,,, 1 ,W G 42409,15/4000uL 3:5,D - - -- 15.0 28 F C 3 G 0 1 3 S 0 2 8 L 1 9 0 6 4 -8 ,, 1 ,W G 42409,15/4000uL 3 :5 ,E - - - 15.0 29 FC 3G 013S 029 L19064-9,, 1 ,W G 42409,15/4000uL 3 :5 ,F - - - 15.0 30 F C 3G 0 1 3 S 0 3 0 L19064-1 0,, 31 F C 3 G 0 1 3 S 0 3 1 L 1 9 0 6 4 - 1 1 ,, 32 F C 3G 013S 032 L19064-12,, 1 ,W G 4 2 4 0 9 ,1 5 /4 0 0 0 u L 3 :6 ,A - - * 15.0 1 ,W G 4 2 4 0 9 ,1 5 /4 0 0 0 u L 3 :6 ,B - - : 15.0 1,W G 4 2 40 9 ,1 5 /40 0 0 u L 3:6, C - - 15.0 33 F C 3 G 0 1 3 S 0 3 3 L 1 9 0 6 4 - 1 3 ,, 1,W G 42409,15/4000uL 3 :6 ,D - - -- 15.0 34 FC 3G 013S034 L19064-14,, 1,W G 42409,15/4000uL 3 :6 ,E - - - 15.0 35 FC3G 013S035 W G 42409-103,,D U P 1,W G 42409,15/4000uL 3 :6 ,F - - 15.0 36 FC 3G 013S036 L19064-15,, 1 ,W G 42409,15/4000uL 3:7,A - - -- 15.0 37 F C 3 G 0 1 3 S 0 3 7 L 1 9 0 6 4 -1 6 ,, 1 ,W G 42409,15/4000uL 3 :7 ,B -- - 15.0 38 F C 3G 0 1 3 S 0 3 8 FC-LI N -4 2 1 5 1 T -D ,,1 /2 1,, 15 u L 39 F C 3G 0 1 3 S 0 3 9 F C -L IN -4 2 1 51 T -D ,, 1/2 1 ,,1 5 u L 40 FC3G 013S040 M eO H ,, 1 ,,15uL 2 :1 ,D 2 :1 ,D 1:8,A - -f V! \ J 15.0 15.0 15.0 At o4c- l i r > L-ro-S e x p i Y ^ ( A cA ^ l s C X L L ^ p l'id le . 2 T A K 2 o L 2 Page 80 of 234 www.axysanalytical.com A X YS METHOD MLA-043 Rev 8 Form 3A PERFLUORINATED ORGANICS INITIAL CALIBRATION PERCENT RECOVERIES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 11 -Jan-2013 CS0 Data Filename: CS1 Data Filename: FC3G_013 S: 4 FC3G_013 S: 5 Instrument ID: LC MS/MS CS2 Data Filename: FC3G_013 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC3G_013 S: 7 C S4 Data Filename: FC3G_013 S: 8 CS5 Data Filename: FC3G_013 S: 9 CS6 Data Filename: FC3G_013 S: 10 CS7 Data Filename: FC3G_013 S: 11 C S8 Data Filename: N/A COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 CS0 102 100 98.7 102 97.9 98.2 93.0 94.0 92.0 100 102 98.2 102 CS1 99.4 105 106 99.9 107 105 119 117 119 102 97.9 108 98.6 CS2 84.6 83.0 88.8 84.3 92.3 97.7 95.0 89.4 103 90.3 89.4 84.8 87.2 P ER C E N T R EC O V ER Y (%) CS3 CS4 CS5 98.7 88.7 101 101 95.1 93.0 98.6 101 110 95.4 93.4 99.6 97.1 110 100 107 99.7 110 111 106 113 116 106 107 103 104 106 116 110 117 104 95.9 101 110 110 111 110 98.0 104 CS6 106 111 104 110 105 103 87.0 104 89.2 102 108 114 105 CS7 93.4 96.2 84.0 86.9 89.5 95.0 71.2 61.7 93.5 92.7 94.9 102 CS8 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_11-Jan-2013_FC3G__Form3A_GS48661.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409-PFOA_FC_LC_11-Jan-2013_FC3G__Form3A_GS48661.html) Page 81 of 234 www.axysanalytical.com A X YS METHOD MLA-043 Rev 8 Form 3B PERFLUORINATED ORGANICS INITIAL CALIBRATION PERCENT RECOVERIES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 11 -Jan-2013 CS0 Data Filename: CS1 Data Filename: FC3G_013 S: 4 FC3G_013 S: 5 Instrument ID: LC MS/MS CS2 Data Filename: FC3G_013 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC3G_013 S: 7 C S4 Data Filename: FC3G_013 S: 8 CS5 Data Filename: FC3G_013 S: 9 CS6 Data Filename: FC3G_013 S: 10 CS7 Data Filename: FC3G_013 S: 11 C S8 Data Filename: N/A LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS CLEAN-UP STANDARD 13C8-PFOA LAB FLA G 1 CS0 87.3 99.1 93.8 93.7 86.8 88.4 85.7 96.7 104 104 CS1 96.2 103 98.1 101 76.9 88.2 81.4 97.1 113 94.3 P ER C E N T R EC O V ER IES (%) CS2 CS3 CS4 CS5 CS6 102 109 101 94.3 87.7 89.8 97.0 97.3 110 82.5 91.7 95.8 90.1 76.2 85.6 84.8 83.3 99.0 103 99.2 100 98.4 95.6 115 121 103 103 115 96.5 103 112 105 133 130 115 94.9 116 97.2 102 108 123 106 92.5 101 93.1 102 103 101 CS7 97.9 105 106 103 149 102 83.9 102 CS8 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3B.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_11-Jan-2013_FC3G__Form3B_GS48661.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_11-Jan-2013_FC3G__Form3B_GS48661.html) Page 82 of 234 www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 3C PERFLUORINATED ORGANICS INITIAL CALIBRATION RETENTION TIMES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 11 -Jan-2013 C S0 Data Filename: CS1 Data Filename: FC3G_013 S: 4 FC3G_013 S: 5 Instrument ID: LC MS/MS C S2 Data Filename: FC3G_013 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC3G_013 S: 7 C S 4 Data Filename: FC3G_013 S: 8 C S5 Data Filename: FC3G_013 S: 9 C S6 Data Filename: FC3G_013 S: 10 C S7 Data Filename: FC3G_013 S: 11 C S8 Data Filename: N/A COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 CS0 5:15 5:59 6:23 6:44 7:09 7:36 8:07 8:40 9:16 6:32 7:23 8:26 9:58 CS1 5:15 6:01 6:23 6:47 7:09 7:36 8:07 8:42 9:18 6:32 7:23 8:26 9:59 CS2 5:16 5:59 6:23 6:44 7:09 7:33 8:03 8:39 9:16 6:31 7:20 8:24 9:59 RETENTION TIMES CS3 CS4 CS5 5:16 5:59 6:22 6:44 7:09 7:33 8:03 8:37 9:15 6:31 7:23 8:24 9:58 5:15 5:58 6:21 6:44 7:09 7:33 8:03 8:37 9:15 6:30 7:23 8:21 9:59 5:15 5:58 6:22 6:47 7:09 7:33 8:03 8:39 9:16 6:31 7:23 8:24 9:59 CS6 5:12 5:57 6:21 6:44 7:09 7:33 8:03 8:37 9:15 6:30 7:20 8:21 9:59 CS7 5:16 5:58 6:21 6:44 7:06 7:29 8:37 9:15 6:29 7:20 8:21 9:59 CS8 MEAN RT 5:15 5:59 6:22 6:45 7:09 7:33 8:04 8:39 9:16 6:31 7:22 8:23 9:59 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3C.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_11-Jan-2013_FC3G__Form3C_GS48661.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409-PFOA_FC_LC_n-Jan-2013_FC3G_Form3C_GS48661.html) Page 83 of 234 www.axysanalytical.com A X Y S METHOD MLA-043 Rev 8 Form 3D PERFLUORINATED ORGANICS INITIAL CALIBRATION RETENTION TIMES AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 11 -Jan-2013 C S0 Data Filename: CS1 Data Filename: FC3G_013 S: 4 FC3G_013 S: 5 Instrument ID: LC MS/MS C S2 Data Filename: FC3G_013 S: 6 L C Colum n ID: C18MS C S3 Data Filename: FC3G_013 S: 7 C S 4 Data Filename: FC3G_013 S: 8 C S5 Data Filename: FC3G_013 S: 9 C S6 Data Filename: FC3G_013 S: 10 C S7 Data Filename: FC3G_013 S: 11 C S8 Data Filename: N/A LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS CLEAN-UP STANDARD 13C8-PFOA LAB FLAG1 CS0 CS1 5:15 6:22 7:09 7:33 8:07 9:16 9:58 8:24 7:23 5:15 6:23 7:09 7:36 8:07 9:18 9:59 8:26 7:23 RETENTION TIMES CS2 CS3 C S4 CS5 C S6 C S7 C S8 MEAN RT 5:17 6:22 7:09 7:33 8:03 9:15 9:58 8:24 7:23 5:15 6:22 7:09 7:33 8:03 9:15 9:58 8:24 7:23 5:16 6:21 7:09 7:33 8:03 9:15 9:58 8:24 7:20 5:16 6:22 7:09 7:33 8:03 9:15 9:59 8:24 7:23 5:14 6:21 7:06 7:33 8:03 8:21 7:20 5:15 6:20 7:06 7:29 8:03 8:24 7:20 5:15 6:22 7:08 7:33 8:04 9:16 9:58 8:24 7:22 7:09 7:09 7:09 7:09 7:09 7:09 7:06 7:06 7:08 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3D.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_11-Jan-2013_FC3G__Form3D_GS48661.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_11-Jan-2013_FC3G__Form3D_GS48661.html) Page 84 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 4A PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 1 1-Ja n -2 0 13 V E R Data Filename: FC3G _013 S: 14 Instrument ID: LC MS/MS A nalysis Date: 1 1-Ja n -2 0 13 L C Colum n ID: C18MS A nalysis Time: 16:10:30 COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 RETEN TION TIME 5:14 5:58 6:22 6:44 7:06 7:29 8:03 8:35 9:13 6:30 7:20 8:21 9:59 EXPECTED CONC. (ng) 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 80.0 80.0 80.0 40.0 CONC. FOUND (ng) 37.6 36.0 40.7 38.8 39.1 37.1 41.2 41.2 45.0 85.3 84.3 80.7 40.5 RECOVERY 94.0 90.0 102 96.9 97.8 92.6 103 103 113 107 105 101 101 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_FC3G_013S14__Form4A_SJ1554620.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_FC3G_013S14__Form4A_SJ1554620.html) Page 85 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 4B PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 1 1-Ja n -2 0 13 V E R Data Filename: FC3G_013 S: 14 Instrument ID: LC MS/MS A nalysis Date: 1 1-Ja n -2 0 13 L C Colum n ID: C18MS A nalysis Time: 16:10:30 LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS CLEANUP STANDARD 13C8-PFOA LAB FLAG 1 RETEN TION TIME 5:15 6:22 7:06 7:29 8:03 9:13 9:59 8:21 7:20 EXPECTED CONC. (ng) 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 CONC. FOUND (ng) 23.1 25.8 78.0 25.7 21.9 22.5 25.1 35.4 36.5 R E C O V E R Y (%) 96.4 108 108 107 91.2 93.7 104 98.3 101 7:06 12.0 13.4 112 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_FC3G_013S14__Form4B_SJ1554620.html; Workgroup: WG42409; DesignID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_FC3G_013S14__Form4B_SJ1554620.html) Page 86 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 4A PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 1 1-Ja n -2 0 13 V E R Data Filename: FC3G _013 S: 38 Instrument ID: LC MS/MS A nalysis Date: 1 1-Ja n -2 0 13 L C Colum n ID: C18MS A nalysis Time: 22:57:42 COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLA G 1 RETEN TION TIME 5:11 5:57 6:20 6:41 7:06 7:28 7:59 8:33 9:09 6:29 7:17 8:17 9:58 EXPECTED CONC. (ng) 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 80.0 80.0 80.0 40.0 CONC. FOUND (ng) 37.3 35.3 40.9 36.8 38.8 37.8 40.0 42.1 45.5 86.3 81.1 75.6 41.3 RECOVERY 93.2 88.2 102 92.0 97.0 94.4 100 105 114 108 101 94.5 103 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_FC3G_013S38_Form4A_SJ1554646.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_FC3G_013S38__Form4A_SJ1554646.html) Page 87 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 4B PERFLUORINATED ORGANICS CALIBRATION VERIFICATION AXYS ANALYTICAL SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial Calibration Date: 1 1-Ja n -2 0 13 V E R Data Filename: FC3G _013 S: 38 Instrument ID: LC MS/MS A nalysis Date: 1 1-Ja n -2 0 13 L C Colum n ID: C18MS A nalysis Time: 22:57:42 LA BELED COMPOUND 13C4-PFBA 13C2-PFHxA 13C2-PFOA 13C5-PFNA 13C2-PFDA 13C2-PFDoA 13C8-PFOSA 13C4-PFOS 18O2-PFHxS CLEANUP STANDARD 13C8-PFOA LAB FLAG 1 RETEN TION TIME 5:11 6:20 7:03 7:28 7:59 9:09 9:59 8:17 7:17 EXPECTED CONC. (ng) 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 CONC. FOUND (ng) 23.3 24.8 72.0 23.0 20.3 18.4 19.8 31.0 31.8 R E C O V E R Y (%) 97.1 103 100 95.7 84.7 76.8 82.5 86.2 88.4 7:03 12.0 12.5 104 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 com pliant quality assurance processes. Signed:____________ Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_FC3G_013S38_Form4B_SJ1554646.html; Workgroup: WG42409; DesignID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_FC3G_013S38__Form4B_SJ1554646.html) Page 88 of 234 www.axysanalytical.com Quantify Sam ple Report M a s sL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnstG\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L1 9064-1,, Name: FC3G_013S021 D escription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 18:09:13 PFBA FC3G_013S021 100- 6.23 (A ,,6.57 %,, /4/.26 I I VV\V: 5.00 6.00 7.00 PFPeA FC3G_013S021 100% 6.68 6.21 5.95 i \\ 7.16 ,-4.33 J \ lil J / \! 5.00 6.00 7.00 PFHxA FC3G013S021 100- 6.38 7.78e2 3442 %- 6.16f 7.07 4f 8 i if'"-' A 5.00 6.00 7.00 PFNA FC3G_013S021 7 cn 100n1 4.38e2"' 1258 7.05 ;v ' r . , i-T-i-!"' . min 7.00 8.00 PFDA FC3G 013S021 100 *, 7.71e3 I 28852 %i * 0 8.00 . - __ _- min 10.00 12.00 PFUnA FC3G013S021 100-n 8.58 1.02e4 . 46666 0 8.00 10.00 12.00 P F H xS -1 FC3G 013S021 7.29;4.71e1;194 100n A6.86 I \ /haU %- j - , , I ,----- , ----- , |f. -T min 7.00 8.00 P FH xS -2 FC3G__013S021 100" , 7.38 A %- I \ j j 7.72 6^20 I I ,-AAI I I M-r.i.m -n min 7.00 8.00 P F H xS -3 FC3G_013S021 100" 6.63 7.46 7.78 - % - 0J \ J 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S021 100- 3 8.71e2 2981 r-\ %- 5.65 - 5.38. . . . ; 3-\- - : ...... min 5.00 6.00 7.00 PFOA-1 FC3G 013S021 100- f6.59e2 / \ 2417 %- . X., 7.84 07.00 ~ min 8.00 P FO A -2 FC3G 013S021 100n J %2 6.63/ \ . 07.00 8.00 PFDoA FC3GJD13S021 100- 9.18 F 7.94e3 I 36027 % PFBS-1 FC3G_013S021 100- 6.73 5.00,.5.79 5.97 7.11 \ i ft te iui P F B S -2 FC3G 013S021 8.00 10.00 12.00 5.00 6.00 7.00 P FO S-1 FC3G 013S021 8.29 100 3.87e4 - ; 136065 %/ i 5 ;; 0- \ 8.00 10.00 m in 12.00 P FO S-2 FC3G 013S021 100- 8.29 I 3.22e4 |l 1 1 7 4 8 4 li 0- b - . , . 8.00 10.00 ^ min 12.00 PFO SA FC3GJD13S021 9.99 1 0 0 n 2.63e2 - 1421 %- 0^ 8.00 10.00 12.00 IC Page 89 of 234 www.axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PR0\FC\FC3G..013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L1 9064-1,, Name: FC3G_013S021 Description: 1,W G42409,15/4000uL Date: 11Oan-2013 Tim e: 18:09:13 1 3 C 2 -P FO A FC3G 013S021 100- 7.09 ^ 2.42e5 963090 %r 1 3 C 8 -P FO A FC3G 013S021 100- 7.09 l 8.86e4 352166 %- 1 3 C 4 -P FO A FC3G 013S021 100- 7.09 ^9 .4 7 e 4 - 373906 %- it 7.00 I m in 8.00 o7.00 m in ' 8.00 I 1111111111111i : 1 7.00 8.00 1 8 0 2 -P FH xS -2 FC3G 013S021 100- 7.29 7 .2 0 e 3 28931 %- 0J - T X T . ---------------- ^ 7.00 8.00 m in 13C4-PFBA FC3G 013S021 100- 5.23 2.32e4 85108 %4.07 111M111M" 'T 777!7^ i r111i 111 m in 5.00 6.00 7.00 1 3 C 2 -P F H x A FC3G 100- 013S021 6.34 6.3 6 e 4 1 296988 1 %- , m [Tf i . !. 11i [ r ,. | n'iTp'iH 1i1. 5.00 6.00 7.00 1 3 C 8 -P FO SA FC3G 013S021 9.96 10(H 9 .9 2 e 3 ~ ] ' 56773 j %- Ia 0 --vni'jj . .,,.,. i|i ------H....-.r..v..U......... in mminin 8.00 10'0 0 124)0 13C2-PFO UEA FC3G 013S021 7.38 100x 2.94e4 ^ . 113722 %: u0--`- n 7.00 i m in 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 1 3 C 4 -P FO S-1 FC3G 013S021 100- 8.35 f 9.38e3 : 33424 13C 4 -P FO S-2 FC3G 013S021 8.29 1 0 0 q T7.76e3 I] 2 9 5 8 8 1 8 0 2 -P FH xS -1 FC3G 013S021 100 7.33 1 .0 6 e 4 ' 43262 ; o: i V m in 8.00 10.00 12.00 1 3 C 5 -P F N A FC3G013S021 100 7.49 8.85o4 . 361042 %- (H m in 8.00 10.00 12.00 1 3 C 2 -P F D A FC3G 100" 013S021 7.99 r c9 .6 4 e4 364666 i a f t S k 0TM 7.00 8.00 13C 2-P FD O A FC3G_013S021 100- 9.18 f 1.37e5 0 575308 %- 7.0 0 8.00 8.00 10.00 12.00 8.00 10.00 12.00 Page 90 of 234 www.axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L1 9064-1,, Name: FC3G_013S021 Description: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 18:09:13 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.19 27 262.98 >218.... 27 312.97> 268.98 6.38 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.55 29 512.96 >468.... 7.99 29 562.96 > 518.... 8.58 30 612.95 >568.... 9.18 24 298.94> 79.96 6.47 25 298.94 > 98.95 24 398.84 > 79.96 7.29 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.99 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.29 21 216.99>171.98 5.23 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.18 21 506>78 9.96 458.98 >393.98 7.38 Area 591 778 871 659 158 438 7713 10168 7942 13 47 38673 32211 263 241523 88625 94653 9384 7759 10612 7204 23238 63631 88521 96450 136709 9918 29407 IS Area 23238 63631 63631 63631 241523 241523 88521 96450 96450 136709 10612 7204 10612 7204 10612 9384 7759 9918 94653 94653 94653 94653 94653 94653 94653 94653 94653 94653 94653 94653 ng/g 0.042 0.058 0.540 0.721 0.423 0.034 56.560 68.149 0.129 39.025 7.314 5.959 15.133 15.347 17.440 17.800 11.698 13.401 13.881 11.314 9.717 7.531 5.074 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0602 0.0342 0.0193 0.0062 0.0171 0.0778 0.1348 0.0405 0.1601 0.0185 0.0314 0.0466 0.0221 0.0557 0.0235 %Rec S/N 7 10 8 35 4 3 130 153 198 5 3 111.1 124.9 101.8 86.2 87.4 99.3 101.4 99.9 114.5 118.6 96.6 83.0 64.3 104.0 804 2041 71 6125 3532 1047 526 330 1332 0 203 2563 1368 697 1402 588 648 Noise 279 199 336 354 69 216 420 223 305 182 24 28 64 38 54 169 58 20 157 100 357 64 90 32 0 419 116 264 523 410 97 176 Height 1924 3442 2981 2417 830 1258 28852 46666 36027 117 194 136065 117484 1421 963090 352166 373906 33424 29588 43262 28931 85108 296988 361042 364666 575308 56773 113722 IS Height 85108 296988 296988 296988 963090 963090 361042 364666 364666 575308 43262 28931 43262 28931 43262 33424 29588 56773 373906 373906 373906 373906 373906 373906 373906 373906 373906 373906 373906 373906 Sample size 2.0500 2.0500 2.0500 2.0500 2.0500 2,0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 2.0500 Factor2 Detection... 1.0 MMI 1.0 1.0 bbl 1.0 bb 1.0 MMI 1.0 MM 1.0 bbl 1.0 bb 1.0 MM 1.0 MM 1.0 MMI 1.0 1.0 MM 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 91 of 234 www.axysanalytical.com Quantify Sam ple Report M a s s L y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L1 9064-2,, Name: FC3G_013S022 Description: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 18:26:14 PFBA FC3G 013S022 100" %- 4/ .07 i,(\v V vfirIVI..! 6.40 l\ 6-55 0- T,^ m;n 5.00 6.00 7.00 PFPeA FC3G 013S022 100- b.yu 2.04e2 | ,6.53 951 %- i 0- ..... .. ,i!'i."ii,i,r,i-rrr~r~H,T"r[";"T"i" mifl 5.00 6.00 7.00 PFHxA FC3G_013S022 100q 6.38 1.23e3 . 5158 7.26 %- 6.06 I \ A 4.69 05.00 6.00 7.00 PFNA FC3G_013S022 2100-, 7.46 4.47e2j 1935 -6.63 %- * PFDA FC3G 013S022 PFUnA FC3G 013S022 7.00 8.00 P F H xS -1 FC3G_013S022 100- A fi T fi 7.24 7.49 : > ,A \V/ V \ M 9? y min 7.00 8.00 P F H xS -2 FC3G 013S022 100j\ \ %- r 7 38 r\ ^ VJ o- T-r-rr min 7.00 8.00 P F H xS -3 FC3G__013S022 100q 7.19 a\ 7.38 ( %- A o: 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S022 100- 6.73 1.09e3 A %- 4.11 3602 H :: -; 6.36 / 5.88. 5.00 6.00 7.00 PFDoA FC3G_013S022 100- 9.18 I 3.41e4 i; 1 4 3 6 9 8 : %- PFOA-1 FC3G_013S022 100- 7.09 . 9.70e2 \ 3441 %\ 7.00 PFBS-1 FC3G 013S022 8.00 PFOA-2 FC3G 013S022 P F B S -2 FC3G_013S022 8.00 10.00 12.00 P FO S-1 FC3G 013S022 100- 8.35 F 2.57e5 I 862328 1 i w8 \ o - VlTirrrri-Ti I r. , i I ri .-r-rp-r m in 8.00 10.00 12.00 P FO S-2 FC3G 013S022 100q 8.35 r 2.01 e5 1 719924 M %- i 1 o : iM a . i, 8.00 10.00 12.00 PFO SA FC3G_013S022 100- 9.99 4.22e3 I 23155 1 %- 8.00 10.00 12.00 Page 92 of 234 www.axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Massiynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L 1 9064-2,, Name: FC3G_013S022 Description: 1,W G42409,15/4000uL Date: 11 ^Jan-2013 Tim e: 18:26:14 13C 2 -P FO A FC3G_013S022 100-1 7.09 2.25e5 912986 13C 8 -P FO A FC3G 013S022 100- 7.09 r 8.98e4 3u4964 %" 13C 4 -P FO A FC3G_ 013S022 100q 7.09 A" 9.22e4 362217 %- 0- -t-7 min 0 illlitik , i 0 h+r,7.00 =rrrf min 8.00 1 8 0 2 -P FH xS -2 FC3G_013S022 ~7 m 100 -6.89e3 27840 - -L i , . it t t t i+rTr-rt min 7.00 8.00 13C4-PFBA FC3G 013S022 100-- '2.2504 87551 %- 4.03 / 0 - Wl-T 5.00 TTfl IIIInTTITTT min 6.00 7.00 1 3 C 2 -P F H x A FC3G_013S022 6.36 1001 6.37e4 275500 '' %- 0" * 5.00 6.00 7.00 13C 8-P FO SA FC3G_013S022 100- 9.96 1.05e4_| 56781 i %- 1 3 C 2 -P FO U E A FC3G013S022 100- - 7.38 3.32e 4 l| 118435, , %- 8.00 10.00 12.00 =n 7.00 8.00 PV by LFIE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S022 100- 8.35 F 7.96e3 2 8 2 '9 ! I 0- . 8.00 10.00 m in 12.00 13C 4 -P FO S-2 FC3G 013S022 100- 8.35 ? 6.82e3 ; 24826 % j, ; 0 -- ----------- -tttt rnin 8.00 10.00 12.00 1 3 C 5 -P F N A FC3G 013S022 100n 7.49 8.31c4 . 321033 B %- ------ -/-- \ min 7.00 8.00 1 3 C 2 -P F D A FC3G 013S022 100- 7.99 f 8.73e4 1 316044 %m m o-: ite.: 8.00 10.00 12.00 1802-PFH xS-l FC3G_013S022 7.33 100- 9.69e3"l 39272 / i % 0 7.00 8.00 ooI 1 1 1 13C 2-P FD O A FC3G_013S022 9.18 I 1.44e5 I 638573 0/o/ _ 8.00 10.00 12.00 OH Page 93 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-2,, Name: FC3GJJ13S022 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:26:14 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFO A-2 7 PFNA 8 PFDA 9 PFUnA 10 PFD oA 11 P F B S -1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFO S-2 18 PFO SA 19 13C 2-PFO A 20 13C 8-PFO A 21 1 3 C 4 -P F O A 22 13C4-PFO S-1 23 13C 4-PFO S-2 24 1802-PFHXS-1 25 1802-P FH X S-2 26 13C 4-PFBA 27 13C2-PFHXA 28 13C 5-PFN A 29 13C 2-PFD A 30 13C2-PFDOA 31 13C 8-P FO S A 32 13C 2-PFO UEA IS# T race RT 26 212 .99> 168.98 5.30 2 7 2 6 2 .9 8 > 2 1 8 . .. 5 .9 0 27 312 .97> 268.98 6.38 27 362.97> 318.98 6.73 19 412 .97> 368.98 7.09 19 4 1 2 .97> 168.80 7.09 28 462.96 > 4 1 8 .... 7.46 29 512 .96 > 4 6 8 .... 7.99 29 562.96 > 5 1 8 .... 8.58 30 612 .95 > 568.... 9.18 24 298 .94> 79.96 25 298.94 > 98.95 6.47 24 398 .84 > 79.96 25 398.84> 98.96 24 3 9 8 .84> 118.70 22 498.93> 79.96 8.35 23 498 .93> 98.96 8.35 31 4 9 8 > 78 9.99 21 4 1 4 .9 7 > 3 6 9 .... 7 .0 9 21 4 2 0 .9 7 > 3 7 5 .... 7 .0 9 4 16 .97 > 3 7 1 .9 7.09 21 503 > 79.96 8.35 21 5 0 3 > 9 8 .96 8.35 21 4 0 2 .9 > 8 4 7.33 21 4 0 2 .9 > 1 0 3 7.33 21 216 .99> 171.98 5.23 21 3 1 4 .9 7 > 2 6 9 .9 8 6 .3 6 21 4 6 7 .9 6 > 4 2 2 .9 7 7 .49 21 5 1 4 .9 6 > 4 6 9 .9 6 7 .9 9 21 6 1 4 .9 5 > 5 6 9 .. .. 9 .1 8 21 506 >78 9.96 458 .98 >393.98 7.38 A re a 451 204 1233 1095 970 162 447 17717 15555 34059 7 256642 200842 4217 224963 89822 92247 7958 6820 9691 6891 22530 63714 83107 87295 143692 10541 33225 IS A rea 22530 63714 63714 63714 224963 224963 83107 87295 87295 143692 9691 6891 9691 6891 9691 7958 6820 10541 92247 92247 92247 92247 92247 92247 92247 92247 92247 92247 92247 92247 ng/g 0.032 0.069 0.026 0.065 1.674 1.261 2.716 4 3 2 .1 3 7 4 7 2 .4 2 8 4 .0 7 0 36.236 7.389 5.643 12.793 13.447 15.877 16.975 11.306 13.377 12.991 10.209 10.182 7.979 5.569 LO D 0.0413 0 .1 4 2 2 0 .0 2 6 6 0 .0 0 5 4 0 .0 0 6 6 0 .0 9 0 5 0 .1 5 0 4 0 .1 2 1 3 0 .3 6 3 5 0 .2 8 2 4 0 .0 2 0 7 0.0181 0.0194 0 .0 1 9 0 0 .1 2 5 4 0.0076 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. % Rec S /N 5 3 18 11 10 0 5 143 307 415 4 1 0 6 .2 1 2 9 .9 99.2 75.0 78.8 93.1 99.5 99.4 1 1 7 .6 1 1 4 .2 89.8 89.5 70.2 1 1 7 .5 4727 2375 416 4227 4227 2555 383 249 405 144 119 2131 2120 1457 1820 262 2197 N oise 429 347 291 327 362 0 392 453 226 346 35 15 236 232 47 182 303 56 216 86 142 74 100 97 193 737 129 151 217 351 217 54 H e ight 2065 951 5158 3602 3441 719 1935 64655 69234 143698 53 862328 719924 23155 912986 364964 362217 28219 24826 39272 27840 87551 275500 321033 316044 638573 56781 118435 IS H e ight S a m ple size 87551 2 .1 1 0 0 275500 2 .1 1 0 0 275500 2 .1 1 0 0 275500 2 .1 1 0 0 912986 2 .1 1 0 0 912986 2 .1 1 0 0 321033 2 .1 1 0 0 316044 2 .1 1 0 0 316044 2 .1 1 0 0 638573 2 .1 1 0 0 39272 2 .1 1 0 0 27840 39272 2 .1 1 0 0 2 .1 1 0 0 27840 2 .1 1 0 0 39272 2 .1 1 0 0 28219 2 .1 1 0 0 24826 2 .1 1 0 0 56781 2 .1 1 0 0 362217 2.1100 362217 2.1100 2.1100 362217 2 .1 1 0 0 362217 2.1100 362217 2.1100 362217 2 .1 1 0 0 362217 2 .1 1 0 0 362217 2 .1 1 0 0 362217 362217 2 .1 1 0 0 2 .1 1 0 0 362217 362217 2 .1 1 0 0 2 .1 1 0 0 2 .1 1 0 0 F a cto r2 D e tectio n... 1.0 M M I 1.0 M M 1.0 M M I 1.0 bb 1.0 M M 1.0 M M 1.0 bbl 1.0 bb 1.0 M M 1.0 M M 1.0 1.0 M M I 1.0 1.0 1.0 1.0 bb 1.0 bb 1.0 M M 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 94 of 234 www.axysanalytical.corn Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-3,, Name: FC3G_013S023 Description: 1,WG42409,15/4000uL Date: 11Jan-2013 Time: 18:43:07 PFBA FC 3G 013S023 100 5.1 9 f4 .1 2 e 2 I 1897 %- 4 .2 6 ^ 6.40 vV \ PFPeA FC3G 100- 013S023 6.03 2.61e2 i 1007 <' 6.68 is ! %- ^ 37 il `\i PFHxA FC3G 013S023 100- 6.33 1.04e3 7.21 P'- 4276 %- 6.06 4.20 - s l.y 5.00 6.00 7.00 5.00 6.00 7.00 5.00 6.00 7.00 PFNA FC3G_013S023 100-] 7.30O2 3171 ~%--6 .6 3 / ^ ^ 0- i i i i 1 i i - i . i . : i . i min 7.00 8.00 PFDA FC3G 013S023 100 i~8.81 e3 A316 0 2 %P J i. . .. 0 ^ =* ^T T fm ^ rfT fn -r^ T ! min 8.00 10.00 12.00 PFUnA FC3G_013S023 10L 8.55 9.21 e3 39709 %- - nCH . . . . 8.00 10.00 12.00 PFHxS-1 FC3G_013S023 100'--\27.3.363e2~g 951 I I %- S.63 7.84 7.00 8.00 PFHxS-2 FC3G_013S023 100- 7.38 1.93e2 638 6.63 k 7.00 8.00 PFHxS-3 FC3G 013S023 PV by LHE, SV by KJC 31-Jan-13, Note: Calver = ng abs. SPM = ng/mL. o o _l_l PFHpA FC3G 013S023 6.68 1 .4 2 e 3 4691 %: 4 .8 2 6 -P8 j A 'lA ' 0 - tttttJ m in 5.00 6.00 7.00 PFOA-1 FC3G 013S023 100] %: 1 o: 7.00 7.72 8.00 PFOA-2 FC3G 013S023 PFDoA FC3G013S023 100- 9.37e3 : 41556 ; l _ .i 8.00 10.00 m m in 12.00 PFBS-1 FC3G 013S023 100%- 6 -2 6 e 2 l 6 .4 0 2928 | 6 .26e2 fc 2526 4.97 0-J^-^W W irn T n ^T r^-tT iT T min 5.00 6.00 7.00 PFBS-2 FC3G 013S023 ioo-q 6.40 4.11 '6 .5 1 6.29 n % -! i 5.844I - Uo i P 5.00 6.00 7.00 PFOS-1 FC3G 013S023 100- 8,29 I 1.28e5 :; 441869 %- o : -- ------------------------- min 8.00 10.00 12.00 PFOS-2 FC3G 013S023 100- 8.29 F 1.01e5 1 365644 j| j- V 0 - 1-fnVrrrTTTTTTT- tttt'i-i m in 8.00 10.00 12.00 PFOSA FC3G 013S023 100- %- 9.99 2 .4 0 e 3 l J119 8 6 ! 0J 8.00 ? * 10.00 12.00 1C Page 95 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-3,, Name: FC3G_013S023 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:43:07 13C2-PFOA FC 3G 013S023 100- ] 7n 2.45e5 976119 13C8-PFOA FC3G 013S023 100n T 9 6 7 e 4 387742 %: 13C4-PFOA FC3G_013S023 100t 7.09 | 1.04e5 - 1417419 %: 7.00 -T-T m in 8.00 1802-PFHxS-2 FC3G_013S023 100- _ 7.29 7.97e3 30547 %- 07.00 -r-r m in 8.00 13C4-PFBA FC3GJJ13S023 100 5.19 2.82e4 104299 07.00 8.00 13C2-PFHxA FC3G 013S023 .. -- -- 7.00 . 8.00 5.00 6.00 7.00 13C8-PFOSA FC3G 013S023 9.96 1001 .1 0 e 4 l 57773 1 %: 1 o: .............. s ................ m in 8.00 10.00 12.00 13C2-PFOUEA FC3G 013S023 7.38 1004.02e4 152155. : %: . J IIII w 0-^ It m in 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S023 100- 8.29 I" 9.66e3 S 36307 %- ft i. 0- 8.00 10.00 m in 12.00 13C4-PFOS-2 FC3G 013S023 100- 8.29 8.53e3 31537 %- 0 V. m in 8.00 10.00 12.00 1802-PFHxS-1 FC3GJJ13S023 7.33 100 n 1.21e4 l\ - 47202 ; %- O-' 7.00 8.00 13C5-PFNA FC3G 013S023 13C2-PFDA FC3G_013S023 . . . 7.99 1001 r 1.06e5 ' 8 399588 13C2-PFDOA FC3G_013S023 100- 9.15 F" 1.76e5 i 790930 %- _ 8.00 10.00 12.00 J L l_ 8.00 10.00 12.00 Page 96 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-3,, Name: FC3GJJ13S023 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:43:07 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 P F D oA 11 P F B S -1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFO S-2 18 PFO SA 19 13C 2-P FO A 20 13C 8-PFO A 21 1 3 C 4 -P F O A 22 13C4-PFO S-1 23 13C 4-PFO S-2 24 1802-PFHXS-1 25 1802-P FH X S-2 26 13C 4-PFBA 27 13C2-PFHXA 28 13C 5-PFN A 29 13C 2-PFD A 30 13C 2-PFD oA 31 1 3 C 8 -P F O S A 32 13C 2-PFO UEA IS# T race RT 26 212.99> 168.98 5.19 27 262 .98 > 2 1 8 .... 6.03 27 312.97> 268.98 6.33 27 362 .97> 318.98 6.68 19 412 .97> 368.98 7.09 19 4 1 2 .97> 168.80 7.09 28 462.96 > 4 1 8 .... 7.55 29 512.96 > 4 6 8 .... 7.99 29 562.96 > 5 1 8 .... 8.55 30 612.95 > 5 6 8 .... 9.15 24 298 .94> 79.96 6.40 25 298.94 > 98.95 24 3 9 8 .8 4 > 79 .9 6 7 .3 3 25 3 9 8 .8 4 > 98.9 6 7.38 24 3 98 .84> 118.70 22 498 .93> 79.96 8.29 23 498 .93> 98.96 8.29 31 4 9 8 > 78 9.99 21 4 1 4 .9 7 > 3 6 9 .... 7 .0 9 21 4 2 0 .9 7 > 3 7 5 .... 7 .0 9 416 .97 > 3 7 1 .9 7.09 21 5 0 3 > 79.9 6 8.29 21 5 0 3 > 9 8 .9 6 8.29 21 4 02 .9> 84 21 4 0 2 .9 > 1 0 3 7.33 7.29 21 2 1 6 .99> 171.98 5.19 21 314 .97> 269.98 6.33 21 4 6 7 .9 6 > 4 2 2 .9 7 7 .4 9 21 5 1 4 .9 6 > 4 6 9 .9 6 7 .9 9 21 6 1 4 .9 5 > 5 6 9 .... 9 .1 5 21 5 0 6 > 7 8 9.96 458 .98 >393.98 7.38 A re a 412 261 1037 1423 1250 198 730 8808 9206 9371 626 236 193 128334 100794 2400 245065 96717 104112 9661 8533 12055 7974 28217 73559 95124 106243 175660 10995 40164 IS A rea 28217 73559 73559 73559 245065 245065 95124 106243 106243 175660 12055 7974 12055 7974 12055 9661 8533 10995 104112 104112 104112 104112 104112 104112 104112 104112 104112 104112 104112 104112 ng/g 0.039 0.091 0 .0 5 3 0.081 0.000 0 .5 7 7 0.581 0.366 0 .6 2 3 0.239 0 .1 3 4 1 8 5 .7 3 3 1 9 7 .6 5 0 2 .2 4 6 36.534 7 .3 6 4 6 .6 5 2 14.374 15.572 18.279 18.180 13.106 14.294 13.762 11.499 11.520 7.703 7.032 LOD 0 .0 1 6 4 0.2827 0 .0 9 3 8 0 .0 7 0 9 0 .0 0 7 8 0 .0 1 9 8 0 .1 7 3 7 0 .0 9 7 0 0 .2 4 8 6 0 .0 3 4 2 0 .2 2 3 3 0 .0 2 0 7 0 .0 4 9 0 0 .0 2 9 8 0 .0 2 2 0 0.0051 0 .0 0 6 0 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. % Rec S /N 9 4 16 16 17 2 24 216 188 188 50 7 0 1 0 2 .5 1 2 4 .0 1 1 2 .0 80.7 87.4 1 0 2 .6 1 0 2 .0 1 1 0 .3 1 2 0 .3 1 1 5 .8 96.8 97.0 64.8 142.1 2469 1599 305 1535 2825 1008 233 444 215 1522 162 2366 856 1087 1763 5984 3531 N oise 222 242 260 285 189 712 132 146 212 221 51 33 139 0 19 179 229 39 636 137 414 156 71 219 20 643 142 452 368 449 10 43 H e ight 1897 1007 4276 4691 3144 1078 3171 31602 39709 41556 2526 951 638 441869 365644 11986 976119 387742 417419 36307 31537 47202 30547 104299 336870 387360 399588 790930 57773 152155 IS H e ig h t 104299 336870 336870 336870 976119 976119 387360 399588 399588 790930 47202 30547 47202 30547 47202 36307 31537 57773 417419 417419 417419 417419 417419 417419 417419 417419 417419 417419 417419 417419 S a m p le size 2 .0 2 0 0 2.0200 2 .0 2 0 0 2.0200 2 .0 2 0 0 2 .0 2 0 0 2 .0 2 0 0 2 .0 2 0 0 2 .0 2 0 0 2 .0 2 0 0 2 .0 2 0 0 2 .0 2 0 0 2.0200 2.0200 2 .0 2 0 0 2 .0 2 0 0 2 .0 2 0 0 2 .0 2 0 0 2.0200 2 .0 2 0 0 2 .0 2 0 0 2.0200 2.0200 2.0200 2.0200 2.0200 2 .0 2 0 0 2.0200 2.0200 2.0200 2.0200 2 .0 2 0 0 F a cto r2 D e tectio n... 1.0 M M I 1.0 MM 1.0 bbl 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 M M 1.0 M M 1.0 bb 1.0 1.0 MM 1.0 MM 1.0 1.0 bb 1.0 bb 1.0 M M 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 97 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\1nst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-4,, Name: FC3G_013S024 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:00:08 PFBA FC3G 013S024 10(F 6.33 6.16 .'% U 4 4 JS.~5~5 413 ;V/! ; l'1 PFPeA FC3G_013S024 , nn 5.88 10 n 7.60e2>> 2198 %1 4 .8jl 2 M ! 6.68 5.00 6.00 7.00 PFNA FC3G 013S024 5.00 6.00 7.00 PFDA FC3G_013S024 100n _ 7.99 7.44e3 26886 PFHxA FC 3G __013S024 6 .33 PFUnA FC3G 013S024 7.00 8.00 PFHxS-1 FC3G 013S024 100 ,6 .6 3 7.33 J T l.1 1 e 2 i 444 8.00 10.00 12.00 PFHxS-2 FC3G 013S024 PFHxS-3 FC3G 013S024 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S024 100- 6.68 9 .5 3 e 2 f; 4513 ; % - 6.46 ' : 4a. 0n 5< -5 .4 7 i 0 - ...............................--...... .......... m in 5.00 6.00 7.00 PFOA-1 FC3GJD13S024 100n 7.09 ` 9.18e2 / 1 3399 %/ \ 7.78 uo ------------------- -----------------i----rmmmin 7.00 8.00 PFOA-2 FC3G_013S024 7.49 1 0 0 - 3.50e2 A 1493 \ %z_ 7.05 I u 0- .-.......................-.r..--....n..-.T...T...-.n.1-r m in 7.00 8.00 PFDoA FC3G_013S024 100- a. io F 5.66e3 24297 TT m in 8.00 10.00 12.00 PFBS-1 FC3G 013S024 100^ 1 .2 2 e 2 l 389 1 %4 .7 4 5 .1 5 \ ^ F A aA -- . 0 ' 11 (7 11 i 111 '. 11 .i i .: mi n 5.00 6.00 7.00 PFBS-2 FC3G 013S024 100n 1 c 5-62 5.11 / 6 .2 5 %: (1 / 7.07 w*ij 0: "iii *!; _ 1L_ 5.00 6.00 7.00 PFOS-1 FC3G 100- 013S024 8.29 r 7.20e3 26066 ; %" PFOS-2 FC3G_013S024 100-q - 8.29 f5 .7 7 e 3 '* 2 1 3 3 3 %: PFOSA FC3G_013S024 100- 9 .9 6 __ 5.63e2 3178 / 8.00 10.00 12.00 T^fTr m in 8.00 10.00 12.00 8.00 10.00 12.00 Page 98 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-4,, Name: FC3G_013S024 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:00:08 13C2-PFOA FC3G 013S024 100-1 7.09 '<~2.00e5 765690 %: 13C8-PFOA FC3G 013S024 100- 7.09 A 7.54e4 295525 13C4-PFOA FC3G 013S024 100- 7.09 8.52e4 314798 %- 0- 7.00 -- min 8.00 07.00 . min 8.00 o7.00 , 8.00 1802-PFHxS-2 FC3G 013S024 13C4-PFBA FC3G__013S024 100- 5.24 i 1.20e4 I 48378 %'4.44 5.00 6.00 7.00 13C2-PFHxA FC3G 013S024 100- 6.34 5.91e4l 279176 | %: o: - - 5.00 6.00 7.00 13C8-PFOSA FC3G_013S024 1nr. 9.96 1001 6.04e3~] 36778 13C2-PFOUEA FC3G 013S024 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S024 100- 8.29 J 9.36c3 35614 % 8.00 10.00 12.00 13C5-PFNA FC3G_013S024 1 AP 100 7 .4 3 e 4 "A 2 9 3 4 0 9 l?l 7.00 m in 8.00 13C4-PFOS-2 FC3G 013S024 100- 8.29 7.3203 ; 27933 %1 :\ 0 V... 8.00 10.00 m in 12.00 13C2-PFDA FC3G 013S024 1 0 0 - I 8.67e4 I 325629 %- H 0 ip & j m in 8.00 10.00 12.00 1802-PFHXS-1 FC3G 013S024 100n 7.29 A 9.44e3 ; -37181 %- / 0J 7.00 V .. 8.00 13C2-PFDoA FC3G_013S024 100-, 9.15 f 1.19e5 I 517853 %- 0 - " 8.00 10.00 12.00 IC& Page 99 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\inst_G\PFC.PR0\FC\FC3G..013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-4,, Name: FC3G_013S024 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:00:08 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 P F D oA 11 P F B S -1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFO SA 19 13C 2-PFO A 20 13C 8-PFO A 21 1 3 C 4 -P F O A 22 13C4-PFO S-1 23 13C 4-PFO S-2 24 1802-PFH XS-1 25 1802-P FH X S-2 26 13C 4-PFBA 27 13C 2-PFH xA 28 13C 5-PFN A 29 13C 2-PFD A 30 13C 2-PFD oA 31 1 3 C 8 -P F O S A 32 13C 2-PFO UEA IS # T race RT 26 212.99> 168.98 5.26 27 262.98 > 2 1 8 .... 5.88 27 312 .97> 268.98 6.33 27 3 62 .97> 318.98 6.68 19 4 12 .97> 368.98 7.09 19 4 1 2 .9 7 > 168.80 28 462 .96 > 4 1 8 .... 7.49 29 512.96 > 4 6 8 .... 7.99 29 562.96 > 5 1 8 .... 8.55 30 612 .95 > 568.... 9.15 24 298.94> 79.96 6.49 25 2 98 .94 > 98.95 24 398 .84 > 79.96 7.33 25 398.84> 98.96 24 3 9 8 .8 4 > 118.70 22 4 98 .93> 79.96 8.29 23 4 98 .93> 98.96 8.29 31 4 9 8 > 78 9.96 21 4 1 4 .9 7 > 3 6 9 .... 7 .0 9 21 4 2 0 .9 7 > 3 7 5 .... 7 .09 4 16 .97 > 371.9 7.09 21 5 0 3 > 7 9 .96 8.29 21 503 > 98.96 8.29 21 4 0 2 .9 > 8 4 7.29 21 402 .9> 103 7.29 21 2 1 6 .9 9 > 1 7 1 .9 8 5 .2 4 21 3 1 4 .9 7 > 2 6 9 .9 8 6 .3 4 21 4 6 7 .9 6 > 4 2 2 .9 7 7 .46 21 5 14 .96 >469.96 7.99 21 6 1 4 .9 5 > 5 6 9 .... 9 .1 5 21 5 0 6 > 7 8 9.96 458 .98 >393.98 7.38 A re a 393 760 1087 953 918 332 7437 8414 5661 122 111 7204 5765 563 199891 75395 85160 9356 7322 9439 6463 11980 59067 74288 86699 119050 6037 31284 IS A re a 11980 59067 59067 59067 199891 199891 74288 86699 86699 119050 9439 6463 9439 6463 9439 9356 7322 6037 85160 85160 85160 85160 85160 85160 85160 85160 85160 85160 85160 85160 ng/g 0 .1 8 8 0 .0 6 2 0 .0 3 6 0.590 0.648 0.281 0 .1 1 2 0.130 10.208 12.433 0.846 35.551 6.848 5.310 16.608 15.941 17.074 17.579 6.638 13.693 12.822 11.195 9.314 5.046 5.345 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0 .0 4 5 7 0 .2 4 2 0 0 .0 4 1 7 0.0811 0 .0 0 8 4 0 .0 4 7 2 0 .1 2 6 2 0.0676 0.1923 0 .5 2 6 6 0.0373 0 .0 4 4 7 0 .0 3 4 9 0.0322 0.0195 0 .0 2 0 2 % Rec S /N 4 6 14 14 4 5 107 122 100 16 2 1 0 2 .2 118.1 91.6 95.5 91.7 98.2 101.1 57.3 118.1 1 1 0 .6 96.6 80.3 43.5 1 1 0 .6 176 271 145 2650 269 1891 1086 391 808 309 41 1407 920 977 1023 1279 792 N oise 320 373 308 319 801 242 281 252 315 242 25 33 251 34 79 148 79 22 289 1100 166 33 71 46 87 1171 198 319 333 506 29 147 H e ight 1408 2198 4252 4513 3399 1547 26886 38339 24297 389 444 26066 21333 3178 765690 295525 314798 35614 27933 37181 26914 48378 279176 293409 325629 517853 36778 116801 IS H e ight S a m p le size 48378 2 .0 7 0 0 279176 2 .0 7 0 0 279176 2 .0 7 0 0 279176 2 .0 7 0 0 765690 2 .0 7 0 0 765690 2 .0 7 0 0 293409 2 .0 7 0 0 325629 2 .0 7 0 0 325629 2 .0 7 0 0 517853 2 .0 7 0 0 37181 2 .0 7 0 0 26914 2 .0 7 0 0 37181 2 .0 7 0 0 26914 2 .0 7 0 0 37181 2 .0 7 0 0 35614 2 .0 7 0 0 27933 2 .0 7 0 0 36778 2 .0 7 0 0 314798 2 .0 7 0 0 314798 2.0700 2 .0 7 0 0 314798 2 .0 7 0 0 314798 2.0700 314798 2.0700 314798 2 .0 7 0 0 314798 2 .0 7 0 0 314798 2 .0 7 0 0 314798 2 .0 7 0 0 314798 2 .0 7 0 0 314798 2 .0 7 0 0 314798 2 .0 7 0 0 2 .0 7 0 0 F a cto r2 D e tectio n... 1.0 M MI 1.0 M M 1.0 M M I 1.0 M M 1.0 M M 1.0 M M -I 1.0 M M I 1.0 bb 1.0 M M 1.0 M M 1.0 M M 1.0 1.0 M M 1.0 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 100 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qid Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-5,, Name: FC3GJJ13S025 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:17:09 PFBA FC3G 013S025 PFPeA FC3G 013S025 100- 6.08 5.90 6.63 1.30e2~ l/ ..6.87 999 1 4.93 f t l i i w v 5.00 6.00 7.00 PFNA FC3GJ313S025 100- 7 .4 9 1 .49e3^| 5940 Ii %- 6.63 8.20 PFDA FC3G 013S025 7.00 PFHxS-1 FC3G 013S025 8.00 PFHxS-2 FC3G 013S025 PFHxA FC3G_013S025 100n 6.34 1 .1 0 e 3 ~ | 5487 %-i 4 .0 5 4 .7 8 j\ 7.21 I V^ ^ ^ T 5.00 6.00 7.00 PFUnA FC3G_013S025 100 _ 8 .5 8 1.54e4 8.00 10.00 12.00 PFHxS-3 FC3G 013S025 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3GJ313S025 100- 6.73 9.67e2 3936 i %- 4 0 3 5 .43 6 38 i ' -l^ V U \ 5.00 6.00 7.00 PFDoA FC3GJ313S025 9.18 10 T S~1.21e4 I 47959 8.00 10.00 12.00 PFOS-1 FC3G_013S025 100- 8.35 4.30e4 - 149463 %- 8.00 10.00 12.00 PFOA-1 FC3G_013S025 100 7.0 9 5.96e2 ; . 2611 6.63 . % 7.90 7.00 PFBS-1 FC3G 013S025 8.00 6.38 PFOS-2 FC3G_013S025 100 8.35 r 3.31 e4 * 119154 8.00 10.00 12.00 PFOA-2 FC3G 013S025 PFBS-2 FC3G_013S025 100 6.5 3 4 .5 6 e 1 ~ ) 328 6.53 0/Q/ . 4.56e1 5.15 .5.43 328 /\ 5.00 6.00 7.00 PFOSA FC3G_013S025 100- 10.02 _ 3.21 e2 1438 %- 10.47 12.32 8 58 _ U m in 8.00 10.00 12.00 Page 101 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-5,, Name: FC3G_013S025 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:17:09 13C2-PFOA FC 3G _013S025 7n 100- 2.34e5 951217 %- m 7.00 -- n___ -r-r m in 8.00 13C8-PFOA FC3G 013S025 100- 8.51 e4 339700 %- 1 S i, 0 - . -- r - -A -r--n m in 7.00 8.00 13C4-PFOA FC3G_013S025 100q - 7.09 , 8.94e4 \ 356871 %- 0 -7.00 8.00 1802-PFHxS-2 FC3G 013S025 7.33 100l7 .4 5 e 3 ^ 1292751 %- 1 Bll 0 1 . , , f f P ^ r T V - r - - m in 7.00 8.00 13C8-PFOSA FC3G_013S025 100n 9.96 1 .0 7 e 4 ~ j 58121 I %- 13C4-PFBA FC3G 013S025 100- 5.21 F 2.70e4 97791 %- 4.09 --U 05.00 6.00 tit m in 7.00 13C2-PFOUEA FC3G_013S025 100- 7.38 - 3.57e4 | " 132790 | I %- 1302-PFHxA FC3G _013S025 6.36 100 7.07e4 336367 % 0 if 5.00 6.00 7.00 8.00 10.00 12.00 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S025 8.35 100^ f 1.01e4 37878 %- ; ,\ 0J 8.00 10.00 m in 12.00 13C4-PFOS-2 FC 3G J)13S 025 100n : 8.29 I 8.43e3 32036 %- 0 m in 8.00 10.00 12.00 1802-PFHxS-l FC3G 013S025 13C5-PFNA FC3G_013S025 7 AO 100 1 8.24e4jk 334947 j %- / 7.00 ' ' ...... *r m in 8.00 13C2-PFDA FC3G 013S025 100 8.81e4 I 321510 %- !i ( H l ............................ m in 8.00 10.00 12.00 13C2-PFDoA FC3G_013S025 100 J 9.18 I 1.57e5 : i: 6 9 2 1 0 0 % 0 '.-- 8.00 10.00 12.00 Page 102 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G__013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January31, 2013 11:46:23 Pacific Standard Time ID: L19064-5,, Name: FC3G_013S025 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:17:09 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFO A-2 7 PFNA 8 PFDA 9 PFUnA 10 PFD oA 11 P F B S -1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFO S-2 18 PFO SA 19 13C 2-PFO A 20 13C 8-PFO A 21 13C 4-P F O A 22 13C4-PFO S-1 23 13C 4-PFO S-2 24 1802-P FH xS -1 25 1802-P FH xS -2 26 13C 4-PFBA 27 13C2-PFHXA 28 13C 5-PFN A 29 13C 2-PFD A 30 13C2-PFDOA 31 1 3 C 8 -P F O S A 32 13C 2-PFO UEA IS# T race RT 26 2 1 2 .9 9 > 1 6 8 .9 8 5.21 27 262.98 > 2 1 8 .... 5.90 27 312 .97> 268.98 6.34 27 362 .97> 318.98 6.73 19 4 12 .97> 368 .98 7.09 19 4 12 .97> 168.80 7.05 28 462.96 > 4 1 8 .... 7.49 29 512 .96 > 468.... 8.05 29 562.96 > 5 1 8 .... 8.58 30 612.95 > 5 6 8 .... 9.18 24 298 .94> 79.96 6.38 25 298.94 > 98.95 6.53 24 398.84 > 79.96 25 398 .84> 98.96 7.33 24 3 98 .84> 118.70 22 498 .93> 79.96 8.35 23 498.93> 98.96 8.35 31 4 9 8 > 78 10.02 21 4 1 4 .9 7 > 3 6 9 .... 7 .0 9 21 4 20 .97 > 375.... 7.09 4 16 .97 > 3 7 1 ,9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.29 21 4 0 2 .9 > 8 4 7.33 21 4 0 2 .9 > 1 0 3 7.33 21 2 1 6 .9 9 > 1 7 1 .9 8 5.21 21 3 1 4 .9 7 > 2 6 9 .9 8 6 .3 6 21 4 6 7 .9 6 > 4 2 2 .9 7 7 .49 21 5 1 4 .9 6 >4 6 9 .9 6 7 .99 21 6 1 4 .9 5 > 5 6 9 .... 9 .18 21 5 0 6 > 7 8 9.96 458 .98 >393.98 7.38 Area 479 130 1102 967 596 80 1493 14267 15437 12077 92 46 78 43024 33135 321 233942 85143 89444 10137 8431 9817 7447 26981 70705 82434 88125 156813 10660 35672 IS A re a 26981 70705 70705 70705 233942 233942 82434 88125 88125 156813 9817 7447 9817 7447 9817 10137 8431 10660 89444 89444 89444 89444 89444 89444 89444 89444 89444 89444 89444 89444 ng/g 0.011 0.040 0.012 0.103 1.278 1.226 0.649 0.065 5 6 .0 7 3 6 2 .0 6 8 0.162 38.499 7.156 5.420 16.648 16.985 16.432 18.741 13.833 15.166 13.165 10.529 11.352 8.244 5.923 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0129 0 .0 5 7 8 0.0126 0.0166 0.0241 0.1081 0 .0 7 7 0 0.1141 0 .3 5 1 6 0 .0 3 4 4 0 .0 4 8 7 0.0127 0 .0 2 5 6 0.0529 0.0380 % Rec S /N 6 4 15 25 6 2 11 194 229 209 17 25 10 1 1 3 .9 1 2 7 .0 96.2 98.5 1 0 0 .5 97.2 1 1 0 .9 1 2 2 .8 1 3 4 .6 1 1 6 .8 93.4 1 0 0 .8 73.2 1 2 6 .2 1096 1001 77 9314 1293 674 433 631 431 0 107 1576 826 2267 1474 639 467 N o ise 271 223 359 157 402 213 529 251 292 229 39 13 76 37 171 136 119 19 102 263 530 88 51 91 0 912 213 405 142 469 91 284 H e ight 1747 999 5487 3936 2611 409 5940 48641 66895 47959 654 328 370 149463 119154 1438 951217 339700 356871 37878 32036 39075 29275 97791 336367 334947 321510 692100 58121 132790 IS H e ight S a m p le size 97791 2 .1 3 0 0 336367 2 .1 3 0 0 336367 2 .1 3 0 0 336367 2 .1 3 0 0 951217 2 .1 3 0 0 951217 2 .1 3 0 0 334947 2 .1 3 0 0 321510 2 .1 3 0 0 321510 692100 2 .1 3 0 0 2 .1 3 0 0 39075 2 .1 3 0 0 29275 39075 29275 2 .1 3 0 0 2 .1 3 0 0 2 .1 3 0 0 39075 2 .1 3 0 0 37878 2 .1 3 0 0 32036 2 .1 3 0 0 58121 356871 356871 2 .1 3 0 0 2 .1 3 0 0 2 .1 3 0 0 2 .1 3 0 0 356871 2 .1 3 0 0 356871 2 .1 3 0 0 356871 356871 356871 2 .1 3 0 0 2 .1 3 0 0 2 .1 3 0 0 356871 2 .1 3 0 0 356871 356871 356871 356871 2 .1 3 0 0 2 .1 3 0 0 2 .1 3 0 0 2 .1 3 0 0 2 .1 3 0 0 F a cto r2 D e tectio n... 1.0 M M I 1.0 M M 1.0 bbl 1.0 bb 1.0 M M I 1.0 M M 1.0 bb 1.0 M M 1.0 M M 1.0 M M 1.0 M M 1.0 M M I 1.0 1.0 M M I 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 103 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-6,, Name: FC3GJM3S026 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:34:02 PFBA FC3G 013S026 PFPeA FC3G_013S026 PFHxA FC3G 013S026 PFNA FC3G_013S026 111111 minq i , ,, ,, 'i, pTH"p","i"i"|"i'i 5.00 6.00 7.00 PFDA FC3G 013S026 PFUnA FC3G__013S026 ,,,,, 8.58 1001 fl.1 7 e 4 : < 51701 PFHxS-1 FC3G 013S026 PFHxS-2 FC3G 013S026 PFHxS-3 FC3GJD13S026 7.00 1 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S026 100* 8.60e2 3419 0//o-- 4.52 5.92 r -X/VIjA v , a vA-v a /| <= m in 5.00 6.00 7.00 PFOA-1 FC3G 013S026 100- 8 .3 2 e 2 ; 3366 %/1 8.08 0 - ........... 7.00 m in 8.00 PFOA-2 F C 3G J13S 026 100-n 7.09 2.01e2 - \ 784 % , 7.61 o: 7.00 8.00 PFDoA FC3G 013S026 PFBS-1 FC3G 013S026 100- 6.46 6.19e2 2777 . % - ; 0- -- :-- Jj-- V -1 - m in 5.00 6.00 7.00 PFBS-2 FC3G 100- 013S026 6.46 3 .2 5 e 2 ~ | 1815 1 %-_ 4.03 / 0 - Wrrmftiw 5.00 i 6.00 7.00 PFOS-1 FC3G_013S026 a on 100n- 4 .3 2 e 4 H 151255 U l. m in 8.00 10.00 12.00 PFOS-2 FC3G 013S026 100- r 3.62e4 j 137664 1 1 M 0J 8.00 10.00 m in 12.00 PFOSA FC3G 013S026 100- 2 .6 8 e 3 ~ | 14943 %: 0J " 8.00 10.00 12.00 m Page 104 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-6,, Name: FC3G_013S026 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:34:02 13C2-PFOA FC 3G 013S026 100n 7.09 , 1.95e5 I 788725 %- 13C8-PFOA FC3G013S026 100- 7.09 7.85e4 1 322576 %- 0 7.00 8.00 1802-PFHxS-2 FC3G 013S026 O-M- 7.00 13C4-PFBA FC3G 013S026 8.00 13C8-PFOSA FC3G 013S026 9.99 1006 .7 3 e 3 l 41583 I %- | 13C2-PFOUEA FC3G 013S026 7.38 1002.52e4 101418 %~ - ' r ; 1! ' `I F 1 i 11! 111 rnin 8.00 10.00 12.00 - lilk , 7.00 8.00 13C4-PFOA FC3GJD13S026 100- 7.09 |~ 8.31e4 i 333309 %- 7.00 8.00 13C2-PFHxA FC3G013S026 100- 6.33 5.42e4 1 256975 1 % ' W ttt 5.00 6.00 7.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3GJ313S026 100- 8.29 9.52e3 35987 %- 13C4-PFOS-2 FC3G013S026 100- 8.29 f 6.84e3 S 26777 1802-PFHxS-1 FC3G_013S026 100- 7.33 ' 9.10e3 ` 36017 | | %- Au 8.00 10.00 12.00 o-Mm 8.00 10.00 12.00 7.00 8.00 13C5-PFNA FC3G_013S026 7 Q 100 6 .7 7 e 4 278050j\ i . . i . i - f r r r r r r r f r A m in 7.00 8.00 13C2-PFDA FC3G 013S026 1 0 0 - 1 8.10e4 i; 3 0 4 4 1 0 %1 i 0 - ^ --------------------------- m in 8.00 10.00 12.00 13C2-PFDoA FC3G_013S026 100q - 9.18 1.23e5 540335 %: 0-* 8.00 10.00 12.00 IC Page 105 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-6,, Name: FC3G_013S026 Description: 1,WG42409,15/4000uL Date: 11^Jan-2013 Time: 19:34:02 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFD oA 11 P F B S -1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFO SA 19 13C 2-PFO A 20 13C 8-PFO A 21 1 3 C 4 -P F O A 22 13C4-PFO S-1 23 13C 4-PFO S-2 24 1802-PFHXS-1 25 1802-P FH X S-2 26 13C 4-PFBA 27 13C 2-PFH xA 28 13C 5-PFN A 29 13C 2-PFD A 30 13C 2-PFD oA 31 1 3 C 8 -P F O S A 32 13C 2-PFO UEA IS# T race RT 26 212.99> 168.98 27 262 .98 > 218.... 27 312 .97> 268.98 6.33 27 362.97> 318.98 6.73 19 4 12 .97> 368 .98 7.05 19 4 1 2 .97> 168.80 7.09 28 4 62 .96 > 418.... 7.46 29 512 .96 > 468.... 7.99 29 562 .96 > 518..,. 8.58 30 612 .95 > 568.... 9.18 24 298.94> 79.96 6.46 25 2 98 .94 > 98.95 6.46 24 3 98 .84 > 79.96 7.33 25 398.84> 98.96 7.33 24 3 9 8 .84> 118.70 22 4 9 8 .93> 79.96 8.29 23 4 9 8 .93> 98.96 8.29 31 4 9 8 > 78 9.99 21 4 1 4 .9 7 > 3 6 9 .... 7 .09 21 4 2 0 .9 7 > 3 7 5 .... 7 .09 4 16 .97 > 3 7 1 .9 7.09 21 5 0 3 > 7 9 .9 6 8.29 21 503 > 98.96 8.29 21 402 .9> 84 7.33 21 402 .9> 103 7.29 21 2 1 6 .9 9 > 1 7 1 .9 8 5 .26 21 3 1 4 .9 7 > 2 6 9 .9 8 6 .3 3 21 4 6 7 .9 6 > 4 2 2 .9 7 7 .49 21 514 .96 > 46 9.9 6 7.99 21 614 .95 > 569.... 9.18 21 5 0 6 > 7 8 9.99 4 5 8 .9 8 >393.98 7.38 A re a 934 860 832 201 1768 10861 11689 8822 619 325 165 58 43210 36210 2680 195145 78535 83131 9518 6844 9096 6455 18860 54250 67679 81022 123142 6725 25160 IS A rea 18860 54250 54250 54250 195145 195145 67679 81022 81022 123142 9096 6455 9096 6455 9096 9518 6844 6725 83131 83131 83131 83131 83131 83131 83131 83131 83131 83131 83131 83131 ng/g 0.056 0.023 0.103 0.175 0 .9 8 4 0 .9 5 5 0.559 0.763 0 .3 3 8 0.201 57.825 8 0 .6 8 7 3.870 33.302 6.845 4.855 16.211 14.297 15.787 16.846 10.027 12.067 11.209 10.038 9.244 5.393 4 .0 2 7 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.2621 0 .1 4 7 4 0 .0 2 4 3 0 .0 0 5 9 0 .0 4 3 7 0 .0 3 1 6 0.1391 0 .0 7 1 9 0 .1 5 9 0 0 .3 3 4 6 0 .0 1 3 2 0 .0 0 6 4 0.0246 0.0376 0.0201 0 .0 0 5 6 % R ec S/N 13 23 49 0 17 108 126 143 93 150 2 36 1 0 2 .2 126.1 89.4 99.5 87.8 96.9 1 0 3 .4 92.3 111.1 1 0 3 .2 92.4 85.1 49.7 89.0 922 412 1455 4138 3572 333 1450 301 650 329 82 3243 5354 1147 807 1240 2154 N o ise 272 276 343 147 69 0 416 361 411 279 30 12 278 8 89 164 334 10 191 90 1000 25 89 55 81 841 79 52 265 670 34 47 H e ight 4550 3419 3366 784 6997 39111 51701 39773 2777 1815 632 293 151255 137664 14943 788725 322576 333309 35987 26777 36017 26787 68865 256975 278050 304410 540335 41583 101418 IS H e ight S a m p le size 68865 2.2100 256975 2 .2 1 0 0 256975 2 .2 1 0 0 256975 2 .2 1 0 0 788725 2 .2 1 0 0 788725 2 .2 1 0 0 278050 2 .2 1 0 0 304410 2.2100 304410 2 .2 1 0 0 540335 2 .2 1 0 0 36017 2 .2 1 0 0 26787 2 .2 1 0 0 36017 2 .2 1 0 0 26787 2 .2 1 0 0 36017 2 .2 1 0 0 35987 2.2100 26777 2 .2 1 0 0 41583 2 .2 1 0 0 333309 2 .2 1 0 0 333309 2.2100 2 .2 1 0 0 333309 2 .2 1 0 0 333309 2 .2 1 0 0 333309 2 .2 1 0 0 333309 2 .2 1 0 0 333309 2 .2 1 0 0 333309 2 .2 1 0 0 333309 2 .2 1 0 0 333309 333309 2 .2 1 0 0 2 .2 1 0 0 333309 2 .2 1 0 0 2 .2 1 0 0 F a cto r2 D etection, 1.0 1.0 1.0 M MI 1.0 M M 1.0 M M 1.0 bb 1.0 M M 1.0 M M 1.0 M M 1.0 bb 1.0 M M 1.0 M M 1.0 M M 1.0 M MI 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 106 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-7,, Name: FC3G_013S027 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:51:03 PFBA FC3G__013S027 100 ] 4 6.18 /, | \ 6 .8 3 %-i / 0 7 1 v n / W rrr..................... ........ .. m in 5.00 6.00 7.00 PFPeA FC3G 013S027 100^ 2 .7 8 e 2 ft 1272 1 6.73 , %- i f 4 '41 W . 0- 5.00 , N m in 6.00 7.00 PFHxA FC3G_013S027 1 0 0 -j 6.40 7.46e2~ 3332 % : 4 -15 6.10 - , 5.06 SA 0J 5.00 A" 6.00 7.00 PFNA FC3G 013S027 PFDA FC3G_013S027 100- 7.99 F 7.43e3 I 26212 /<H- a 1 8.00 10.00 12.00 PFUnA FC3GJJ13S027 100- 8.55 6.99e3 30880 h PFHxS-1 FC3G 013S027 PFHxS-2 FC3G__013S027 100 - 6 -63 PFHxS-3 FC3G 013S027 %- l \ 7.29 7.92e0 55 8.08 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA aF C 3 G _ 0 1 3 S 0 2 7 100- 6 4302 3117 ' 5 .5 2 5 .6 4 '\ : 5.00 6.00 7.00 PFDoA FC3G 013S027 PFOS-1 FC3G_013S027 mn 100n 8-29 F 9.12e3 1 32017 %1 J 8.00 10.00 12.00 PFOA-1 FC3G 013S027 1 0 -J %/ \ 7.90 ' i i i .......................... . V m in 7.00 8.00 PFBS-1 F C 3 G ..0 1 3 S 0 2 7 100%- 6.53 1 . 9 9 e l l 6 -53 1 5 1 |1.99e1 151 4.89 6.92 MM. 5.00 6.00 7.00 PFOS-2 FC 3G J13S 027 100- 8.29 7.24e3 26000 %- PFOA-2 FC3G_013S027 100q 7.14 2.19e2 . 783 %8.02 0J 7.00 PFBS-2 FC3G 013S027 8.00 PFOSA FC3G_013S027 100- 9 .9 6 _ 3.10e2 I 1942 I %- frtW r 8.00 10.00 12.00 0-+T 8.00 10.00 12.00 m Page 107 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-7,, Name: FC3G_013S027 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:51:03 13C2-PFOA FC3G 013S027 100- 7.09 I.89e5 728266 %- 13C8-PFOA FC3G 013S027 100" 7.09 Ih 7.60c4 298632 %: 13C4-PFOA FC3G 013S027 100" 7.09 r 8.12e4 317673 %- l i f t 0 - ft*!------- , - -p ~-rT--iTmminin 7.00 8.00 u0--^1rr ir n T r p - m n T-T mmmin 7.00 8.00 u0-1*^ ^ 7TTT^r|i i FPjTn 7.00 8.00 1802-PFHxS-2 FC3G_013S027 100- 7.29 Ji6.19e3 24515 %- 13C4-PFBA FC3G 013S027 13C2-PFHxA FC3G 013S027 7.00 8.00 13C8-PFOSA FC3G 013S027 100- 9.96 5.75e3l : 33818 S %z 13C2-PFOUEA FC3G 013S027 100" 7.38 2.85e4 104737 %- o: ..... . 1I 1 '*""^1 i i i . Mi n min 8.00 10.00 12.00 0-1 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S027 100- 8.29 l 8.77e3 . 34158 : 08.00 min 10.00 12:00 13C4-PFOS-2 FC3G 013S027 100- 8.29 F 6.62e3 1 24410 %fl 0 - hi k r r r ........- min 8.00 10.00 12.00 1802-PFHxS-l FC3G 013S027 1 00- 7.33 8.73e3 35598 %- 0- / 7.00 8 13C5-PFNA FC3G 013S027 100- 7.49 6 .8 9 e 4 ^ 271280 1 %- 13C2-PFDA FC3G 013S027 7.99 1 00 - I 7.86e4 1 279350 %- 0^ 7.00 m|n 8.00 0J 8.00 10.00 12.00 13C2-PFDoA FC3G 013S027 100- 9.15 8.10e4 356459 %J 1 ! o: jt i f 8.00 10.00 12.00 m Page 108 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset G:\Masslynx\lnstJ3\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-7,, Name: FC3G_013S027 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:51:03 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 P F B S -1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFO SA 19 13C 2-P FO A 20 13C 8-PFO A 21 1 3 C 4 -P F O A 22 13C4-PFO S-1 23 13C 4-PFO S-2 24 1802-P F H xS -l 25 1802-P FH X S-2 26 13C 4-PFBA 27 13C 2-PFH xA 28 13C 5-PFN A 29 13C 2-PFD A 30 13C2-PFDOA 31 13C 8-P FO S A 32 13C 2-PFO UEA IS# T race RT 26 212.99> 168.98 5.26 27 262.98 > 2 1 8 .... 5.97 27 3 1 2 .97> 268.98 6.40 27 362 .97> 318.98 6.73 19 4 12 .97> 368.98 7.09 19 4 12 .97> 168.80 7.14 28 462 .96 > 4 1 8 .... 7.49 29 512 .96 > 4 6 8 .... 7.99 29 562 .96 > 518.... 8.55 30 612.95 > 5 6 8 .... 9.18 24 2 9 8 .94> 79.96 6.53 25 298 .94 > 98.95 24 398 .84 > 79.96 25 3 9 8 .84> 98.96 7.29 24 3 9 8 .84> 118.70 22 498 .93> 79.96 8.29 23 4 9 8 .93> 98.96 8.29 31 4 9 8 > 78 9.96 21 4 1 4 .9 7 > 3 6 9 .... 7 .09 21 4 2 0 .9 7 > 375.... 7.09 4 16 .97 > 3 7 1 .9 7.09 21 5 0 3 > 7 9 .9 6 8.29 21 5 0 3 > 9 8 .9 6 8.29 21 4 0 2 .9 > 8 4 7.33 21 4 0 2 .9 > 1 0 3 7.29 21 2 1 6 .9 9 > 1 7 1 .9 8 5.21 21 3 1 4 .9 7 > 2 6 9 .9 8 6 .3 4 21 4 6 7 .96> 422.97 7.49 21 5 1 4 .9 6 > 4 6 9 .9 6 7 .9 9 21 6 1 4 .9 5 > 5 6 9 .... 9 .1 5 21 5 0 6 > 7 8 9.96 458 .98 >393.98 7.38 A re a 655 278 746 643 583 219 701 7427 6993 4453 20 8 9123 7242 310 189082 76015 81235 8774 6619 8732 6187 22264 54666 68870 78561 81044 5751 28458 IS A re a 22264 54666 54666 54666 189082 189082 68870 78561 78561 81044 8732 6187 8732 6187 8732 8774 6619 5751 81235 81235 81235 81235 81235 81235 81235 81235 81235 81235 81235 81235 ng/g 0.066 0.026 0.134 0.027 0.701 0.609 0.393 14.455 18.156 0 .4 4 0 36.671 7.529 5.269 16.984 15.714 17.225 18.351 13.452 13.819 12.962 11.062 6.914 5.242 5.058 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0 .0 3 2 9 0.0276 0 .0 1 8 5 0 .0 1 9 0 0 .0 1 0 7 0 .0 6 9 6 0 .1 5 0 6 0 .1 0 0 3 0.1541 0 .5 0 0 6 0 .0 2 7 6 0 .0 2 2 9 0 .0 3 0 9 0 .0 4 8 4 0 .0 1 3 5 0 .0 1 5 4 % Rec S /N 9 5 10 15 4 7 7 107 130 116 8 3 1 0 1 .4 1 2 4 .9 87.4 93.9 86.9 95.2 1 0 1 .4 1 1 1 .5 114.6 1 0 7 .5 91.7 57.3 43.5 1 0 0 .7 332 247 124 5872 1194 1482 729 295 537 362 79 1732 1713 978 482 1751 988 N oise 238 257 350 204 311 107 380 245 237 179 20 6 375 18 70 97 105 16 124 250 214 47 83 66 68 1080 142 158 286 740 19 106 H e ight 2240 1272 3332 3117 1242 783 2648 26212 30880 20764 151 55 32017 26000 1942 728266 298632 317673 34158 24410 35598 24515 84849 246658 271280 279350 356459 33818 T04737 IS H e ight S a m p le size 84849 1.9900 246658 1.9900 246658 1.9900 246658 1.9900 728266 1.9900 728266 1.9900 271280 1.9900 279350 1.9900 279350 1.9900 356459 1.9900 35598 1.9900 24515 35598 1.9900 1.9900 24515 1.9900 35598 1.9900 34158 1.9900 24410 1.9900 33818 1.9900 317673 1.9900 317673 1.9900 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 1.9900 F actor2 D e tectio n... 1.0 M M I 1.0 M M 1.0 M M I 1.0 bb 1.0 M M I 1.0 M M 1.0 bb 1.0 bb 1.0 M M 1.0 bb 1.0 M M I 1.0 1.0 1.0 M M I 1.0 1.0 bb 1.0 bb 1.0 M M 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 109 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnstJ3\PFC.PRO\FC\FC3GJ)13-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-8,, Name: FC3GJJ13S028 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:07:56 PFBA FC 3G 013S028 100- 6.25 6.36 4.05 j I'M / \ PFPeA FC3G 013S028 o- 5.00 6.00 7.00 PFNA FC3G 013S028 PFDA FC3G_013S028 PFHxA FC3G 013S028 PFUnA FC3G 013S028 7.00 PFHxS-1 FC3G_013S028 8.00 PFHxS-2 FC3G_013S028 PFHxS-3 FC3G 013S028 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S028 PFOA-1 FC3G 013S028 PFOA-2 FC3G 013S028 PFDoA FC3G_013S028 100-, _ 9.12 j 4.44e3 i 17756 %- !; 8 .5 8 ?> --- 8.00 10.00 12.00 PFOS-1 FC3G_013S028 100-, 8.29 9.01 e3 30840 %- o^Uf 8.00 10.00 12.00 PFBS-1 FC3G 100q 013S028 6.47 4.59e1 500 %- 6.7 3 6.23 4.57 MV . A . IV* \ f T h T fr^fY i^T ^T r 5.00 6.00 7.00 . P F O S -2 FC3G_013S028 100n 8.29 6.88e3 26051 %- PFBS-2 FC3G_013S028 100q 6.46 4.06e1 392 %- 4.44 ,6.87 6.06 \A P"TT"v,r n'r' 5.00 6.00 7.00 PFOSA FC3G_013S028 100- 9.9 3 1 .1 8 e 3 ~ l 6237 j %- 8.00 10.00 12.00 o-v _ 8.00 10.00 12.00 Page 110 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnstJ3\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-8,, Name: FC3GJJ13S028 Description: 1,WG42409,15/4000uL Date: 11>Jan-2013 Time: 20:07:56 13C2-PFOA FC3G 013S028 100- 7.09 2.10e5 859509 %T 13C8-PFOA FC3G 013S028 100-j 7.09 " 7.96e4 317815 %: 13C4-PFOA FC3G_013S028 100q 7.09 8.72e4 353756 %: 0 -L-hA----------- tt , i . min 7.00 8.00 0- 7.00 | "tt min 8.00 0- 7.00 8.00 1802-PFHxS-2 FC3G_013S028 100- 7.3 3 _ ' J 6 .6 7 e 3 l ` 26962 l | 13C4-PFBA FC3GJM 3S028 100- 5.21 I" 1.73e4 1 67531 13C2-PFHxA FC3G 013S028 7.00 8.00 13C8-PFOSA FC3G_013S028 100- 9.9 3 6.55e3 j 38092 %- 4.09 i-- :-- :-- r w " 5.00 6.00 7.00 13C2-PFOUEA FC3G 013S028 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S028 100- 8.29 9.56e3 36848 %- 1 3C 4-P F O S -2 FC3G_013S028 100- 8.29 f 8.05e3 I 31110 %- 1802-PFHXS-1 FC3G_013S028 100-I l. 7.33 00e 4 'l ` 41294 %- 8.00 10.00 12.00 13C5-PFNA FC3G 013S028 8.00 10.00 12.00 13C2-PFDA FC3G_013S028 100- 7.99 9.40 e4 337660 %- ; ;\ i 8.00 10.00 12.00 7.00 8.00 13C 2-P F D oA FC3G__013S028 inn 9/15 100 T 1.11e5 1 477070 %- 8.00 10.00 12.00 m Page 111 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-8,, Name: FC3G_013S028 Description: 1,WG42409,15/4000uL Date: 11>Jan-2013 Time: 20:07:56 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.15 27 262.98 >218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.49 29 512.96 >468.... 8.05 29 562.96 >518.... 8.55 30 612.95 >568.... 9.12 24 298.94> 79.96 6.47 25 298.94 > 98.95 6.46 24 398.84 > 79.96 25 398.84> 98.96 7.29 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.93 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.21 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.93 458.98 >393.98 7.38 Area 439 290 965 958 721 238 480 4560 5852 4440 46 41 26 9005 6878 1179 209723 79588 87243 9559 8048 9999 6671 17346 57236 76367 93971 111210 6546 31775 IS Area 17346 57236 57236 57236 209723 209723 76367 93971 93971 111210 9999 6671 9999 6671 9999 9559 8048 6546 87243 87243 87243 87243 87243 87243 87243 87243 87243 87243 87243 87243 ng/g 0.061 0.065 0.123 0.230 0.366 0.176 0.006 12.265 13.213 1.740 35.551 6.890 5.311 16.172 16.701 17.238 17.293 9.161 12.646 12.563 11.565 8.293 5.215 5.301 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0295 0.0261 0.0474 0.0238 0.0174 0.0635 0.1405 0.0993 0.4506 0.2291 0.0380 0.0259 0.0138 0.0266 0.0269 0.0076 %Rec S/N 4 5 13 23 6 0 7 91 181 100 6 17 3 104.7 121.7 93.8 95.2 98.3 101.5 101.8 80.9 111.7 111.0 102.2 73.3 46.1 112.4 257 461 496 2273 856 914 726 340 531 115 115 1129 1402 2228 988 835 2097 Noise 556 266 301 197 399 0 264 167 151 177 86 23 221 30 0 120 56 13 378 371 387 51 92 78 235 586 232 212 152 483 46 57 Height 2139 1198 3781 4554 2273 1295 1791 15137 27357 17756 500 392 91 30840 26051 6237 859509 317815 353756 36848 31110 41294 26962 67531 262250 297757 337660 477070 38092 120193 IS Height 67531 262250 262250 262250 859509 859509 297757 337660 337660 477070 41294 26962 41294 26962 41294 36848 31110 38092 353756 353756 353756 353756 353756 353756 353756 353756 353756 353756 353756 353756 Sample size 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 bbl 1.0 bb 1.0 bbl 1.0 MM 1.0 MMI 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MMI 1.0 1.0 MMI 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 112 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-9,, Name: FC3G_013S029 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:24:57 PFBA FC3G 013S029 100- I 6.31 A An \ ..6.51 %- f !V ^ \/.1 6 5.00 6.00 7.00 PFPeA FC 3G 013S029 100- 6.03 3 .2 9 e 2 l 1726 I 6.57 %- 4.7 0 'J H * 5.00 6.00 7.00 PFNA FC3G 013S029 PFDA FC3G013S029 100- 8.05 f 9.52e3 1 33264 %- PFHxS-1 FC3G 013S029 ' JB ll f^rrrrTfiTTt" r 8.00 10.00 12.00 PFHxS-2 FC3G 013S029 PFHxA FC3G_013S029 100- 6.38 _ 1,06e3 5748 ri 1 4 .1 5 _ / 5.69 f i --'W vx/V'. 7.21 5.00 6.00 7.00 PFUnA FC3G013S029 100- _ 8.58 F 1.09e4 I 44403 %- J L T frif m in 8.00 10.00 12.00 PFHxS-3 FC3G 013S029 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G013S029 100n 6.68 1.06e3 3713 I 4 03 5.15 e -14 ...\ 5.00 6.00 7.00 PFOA-1 FC3G_013S029 100- 7.14 9 . 8e2 I 3080 %- V p 7.96 7.00 8.00 PFOA-2 FC3G 013S029 PFDoA FC3G 013S029 PFBS-1 FC3G 013S029 100- 6.46 1 .5 4 e 3 ?. 6261 : %- PFBS-2 FC3G 013S029 100- 6.44 7 .2 9 e 2 ~ | 3294 p 5.00 M i i i Iii 6.00 7.00 5.00 6.00 PFOS-1 F C 3 G _ 0 1 3 Sa0 2ri9e 100 "" 1.9265 5 588070 /V min 8.00 10.00 12.00 PFOS-2 FC3G 013S029 100 1.42e5 476013 % \\ ! 0 8.00 ------------------- min 10.00 12.00 PFOSA FC3G 013S029 100q 4 .4 3 e 4 - 243228 1 %: 0-" i " " i ' r 8.00 10.00 12.00 Page 113 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3GJD13-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-9,, Name: FC3G_013S029 Description: 1,WG42409,15/4000uL Date: HnJan-2013 Time: 20:24:57 13C2-PFOA FC3G013S029 100 ] I.8 1 e 5 716397 - 13C8-PFOA FC3G 013S029 100- i 7.07e4 277888 %- o o _J_J 13C4-PFOA FC3GJ313S029 7.09 i 7.70e4 ; 1306024 %~ 7.00 -T-T m in 8.00 1802-PFHxS-2 FC3G 013S029 0 - -----7.00 ~7~V-l..I'- n m in 8.00 13C4-PFBA FC3G_013S029 100- 5.21 2.40 e4 94855 % -4.0 7 qJ 7.00 13C2-PFHxA FC3G 013S029 8.00 13C8-PFOSA FC3G_013S029 mn 99 9 100n 7.93e3~l 45487 | %~l it 5.00 6.00 7.00 13C2-PFOUEA FC3G 013S029 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S029 10(F] 8.35 7.01e3 : 26814 %J ; 0^ m in 8.00 10.00 12.00 1 3C 4-P F O S -2 FC3G_013S029 100q - 8.35 F 6.71e3 I 25102 %- 0 -1M 8.00 10.00 12.00 13C5-PFNA FC3G_013S029 13C2-PFDA FC3G_013S029 100- 7.99 7.52e4 265288 %- 8.00 10.00 12.00 1802-P F H xS -1 FC3G_013S029 100- 7.3 3 -8 .6 5 e 3 35036 ; \ %- 7.00 8.00 13C2-PFDOA FC3G_013S029 100- 9.18 1.16e5 524026 %- 8.00 10.00 12.00 Page 114 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday. January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-9,, Name: FC3G_013S029 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:24:57 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFO A-2 7 PFNA 8 PFDA 9 PFUnA 10 P F D oA 11 P F B S -1 12 PFBS-2 13 PFHxS-1 14 P FH xS-2 15 PFHxS-3 16 PFOS-1 17 PFO S-2 18 PFOSA 19 13C 2-PFO A 20 13C 8-PFO A 21 13C 4-P FO A 22 13C4-PFO S-1 23 13C 4-PFO S-2 24 1802-PFHXS-1 25 1802-P FH X S-2 26 13C 4-PFBA 27 13C2-PFHXA 28 13C 5-PFN A 29 13C 2-P FD A 30 13C 2-PFD oA 31 13C 8-P F O S A 32 13C 2-PFO UEA IS# T race RT 26 212 .99> 168.98 5.13 2 7 2 6 2 .9 8 > 2 1 8 . .. 6 .0 3 27 312.97> 268.98 6.38 27 362 .97> 318.98 6.68 19 4 1 2 .9 7 > 368 .98 7.14 19 4 1 2 .97> 168.80 7.09 28 462 .96 > 4 1 8 .... 7.46 29 512.96 > 4 6 8 .... 8.05 29 562.96 > 5 1 8 .... 8.58 30 612.95 > 5 6 8 .... 9.18 24 2 98 .94> 79.96 6.46 25 298.94 > 98.95 6.44 24 398 .84 > 79.96 7.33 25 3 98 .84> 98.96 7.33 24 3 9 8 .8 4 > 118.70 7.29 22 4 9 8 .93> 79.96 8.35 23 4 9 8 .93> 98.96 8.35 31 4 9 8 > 78 9.99 21 4 1 4 .9 7 > 3 6 9 .... 7 .09 21 4 20 .97 > 375.... 7.09 4 16 .97 > 3 7 1 .9 7.09 21 5 0 3 > 79 .9 6 8.35 21 5 0 3 > 98 .9 6 8.35 21 4 0 2 .9 > 8 4 21 4 0 2 .9 > 1 0 3 7.33 7.33 21 216 .99> 171.98 5.21 21 3 1 4 .9 7 > 2 6 9 .9 8 6 .3 4 21 4 6 7 .9 6 > 4 2 2 .9 7 7 .4 9 21 5 1 4 .9 6 > 4 6 9 .9 6 7 .9 9 21 6 1 4 .9 5 > 5 6 9 .... 9 .1 8 21 506>78 9.99 458 .98 >393.98 7.38 A re a 580 329 1061 1056 908 136 377 9522 10853 8757 1536 729 575 305 189 191824 141946 44276 180728 70744 77041 7010 6708 8654 5844 24010 60045 56653 75219 115837 7925 25845 IS A re a 24010 60045 60045 60045 180728 180728 56653 75219 75219 115837 8654 5844 8654 5844 8654 7010 6708 7925 77041 77041 77041 77041 77041 77041 77041 77041 77041 77041 77041 77041 ng/g 0 .0 6 4 0.068 0.046 0 .0 6 5 0.905 0 .9 4 2 0.600 2.040 1.464 0.783 0.554 0.550 3 4 5 .3 3 0 3 1 9 .6 4 8 55.423 3 2 .8 3 4 6.564 4.439 12.711 14.918 15.992 16.237 13.590 14.219 9.989 9.921 9 .2 5 8 6.766 4.081 LOD 0 .0 0 7 5 0 .0 7 3 9 0 .0 7 0 6 0 .1 0 6 8 0 .0 0 8 4 0.0243 0.0095 0.0744 0.0731 0 .0 8 8 2 0.0119 0.2418 0.0209 0.0438 0 .0 3 1 0 0 .0 4 3 7 0 .0 1 7 0 0 .0 1 3 2 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. % Rec 1 0 2 .2 1 2 2 .5 82.9 79.1 92.8 99.5 1 0 1 .0 1 2 6 .8 1 3 2 .7 93.2 92.6 86.4 63.2 91.4 S /N 8 12 21 18 16 3 3 117 197 192 53 246 18 14 9 5655 2008 1753 4051 2044 179 502 478 4112 0 168 2280 616 851 723 1730 927 N oise 278 148 278 201 189 195 437 285 226 201 118 13 124 110 72 104 237 139 177 136 1709 53 53 9 0 566 117 329 312 724 26 113 H e ight 2156 1726 5748 3713 3080 545 1391 33264 44403 38464 6261 3294 2186 1583 633 588070 476013 243228 716397 277888 306024 26814 25102 35036 22950 94855 266205 202602 265288 524026 45487 104582 IS H e ight S a m p le size 94855 2 .2 4 0 0 266205 2 .2 4 0 0 266205 2 .2 4 0 0 266205 2 .2 4 0 0 716397 2 .2 4 0 0 716397 2 .2 4 0 0 202602 2.2400 265288 265288 2 .2 4 0 0 2.2400 524026 2.2400 35036 2.2400 22950 2.2400 35036 2 .2 4 0 0 22950 2 .2 4 0 0 35036 2 .2 4 0 0 26814 2 .2 4 0 0 25102 2 .2 4 0 0 45487 2 .2 4 0 0 306024 2 .2 4 0 0 306024 2 .2 4 0 0 2 .2 4 0 0 306024 2 .2 4 0 0 306024 2 .2 4 0 0 306024 306024 306024 2 .2 4 0 0 2 .2 4 0 0 2 .2 4 0 0 306024 2 .2 4 0 0 306024 2 .2 4 0 0 306024 306024 2 .2 4 0 0 2 .2 4 0 0 306024 2.2400 2 .2 4 0 0 F a cto r2 D etection... 1.0 M MI 1.0 MM 1.0 bbl 1.0 bb 1.0 M M 1.0 M M 1.0 M MI 1.0 M M 1.0 M M 1.0 M M 1.0 bb 1.0 bb 1.0 M M 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 M M 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 115 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-10,, Name: FC3G_013S030 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:41:50 to o PFBA FC3G_013S030 100 V 6.57 1A M . >/ M i A j'f ' I.mi n 5.00 6.00 7.00 PFNA FC3G 013S030 PFPeA FC3G 013S030 100- 5 .6 5 ^ 4.29 %- ! ii 4 .9 3 V o- 5.00 u, 4V -rrrrrrrnTTTTTTTr m in 6.00 7.00 PFDA FC3G_013S030 100 _ 7.9 9 9.07e3 ! 32245 PFHxA FC3G013S030 100-1 6.34 _ 7.72e2 I a3 0 4 9 %- ,, ,,,, 4.20 5-99 \ o: 5.00 6.00 7.00 PFUnA FC3G 013S030 PFHxS-1 FC3G 013S030 8.00 10.00 12.00 PFHxS-2 FC3G 013S030 PFHxS-3 FC3G 013S030 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S030 PFOA-1 FC3G_013S030 100- 7.09 JT 7.23e2 1 2910 %- 7.78 PFOA-2 FC3G 013S030 7.00 8.00 PFDoA FC3G 013S030 PFBS-1 FC3G_013S030 1 0 0 ^ . 0 3 5 ' ' 9^ - 77 ,5 .9 0 4.85 i ii 7.21 *y\ 0-C M u 5.00 6.00 7.00 PFBS-2 FC3G 013S030 PFOS-1 FC3G_013S030 a oo 100- 1 j~ 1,42e4 1 50045 i%- m in 8.00 10.00 12.00 P F O S -2 FC3G 013S030 100%- 1.19e4 1 45058 1 0- 8.00 ........................ m in 10.00 12.00 PFOSA FC3G_013S030 100-] 10.05 7.85e1 : 418 %8.61 q , I 9f 1 | 11.3611.75 0-* . Ill' 1 4 I 1r.f r f m in 8.00 10.00 12.00 m Page 116 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-10,, Name: FC3G_013S030 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:41:50 13C2-PFOA FC3G 013S030 13C8-PFOA FC3G 013S030 13C4-PFOA FC3G_013S030 100- 7.09 7.50e4 296161 %- 1802-PFHxS-2 FC3G_013S030 100- 7.29 5.85o3 23858 %- 13C4-PFBA FC3G 013S030 7.00 8.00 13C2-PFHxA FC3G_013S030 100- 6.34 6.31 e4 282955 %- 7.00 8.00 13C8-PFOSA FC3G 100- 013S030 9.96 9 .5 4 e 3 l 50076 n 13G2-PFOUEA FC3G 013S030 7.38 1002.92e4 109965 0 - ... ............ ... m in 8.00 10.00 12.00 0 - 1- ^ 4 7.00 8.00 5.00 6.00 7.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC 3G 013S030 100- 8.29 F 8.89e3 I 33822 %- 13C 4-P F O S -2 FC3G__013S030 100 . 7862e3 ;; 2 7 9 3 7 1802-PFHxS-1 FC3G 013S030 -- ~----------- --------f 8.00 10.00 12.00 8.00 10.00 12.00 13C5-PFNA FC 3G 013S030 7 AO 100-1 7.0 7e4 283341 R %- 7.00 **t m in 8.00 13C2-PFDA FC3G 013S030 100- ' 8.27e4 S 303512 IB %- 0 - ... ...... m in 8.00 10.00 12.00 13C 2-P F D oA FC3G_013S030 100q 9.15 F 1.41e5 1 611461 %- 0: 8.00 10.00 12.00 Page 117 of 234 www.axysanalytical.com Q uantify Sam ple R eport M assL ynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-10,, Name: FC3G_013S030 D escription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 20:41:50 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 P F B S -1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFO S-2 18 P FO SA 19 13C 2-PFO A 20 13C 8-PFO A 21 13C 4-P FO A 22 13C4-PFO S-1 23 13C 4-PFO S-2 24 1802-PFHXS-1 25 1802-P FH X S-2 26 13C 4-PFBA 27 13C2-PFHXA 28 13C 5-PFN A 29 13C 2-PFD A 30 13C 2-PFD oA 31 13C 8-P FO S A 32 13C 2-PFO UEA IS# T race RT 26 212.99>168.98 5.28 27 262.98 > 2 1 8 .... 27 312 .97> 268.98 6.34 27 362.97> 318.98 19 4 1 2 .97> 368.98 7.09 19 4 1 2 .97> 168.80 7.09 28 462 .96 > 4 1 8 .... 7.49 29 512.96 > 468.... 7.99 29 562 .96 > 5 1 8 .... 8.55 30 612 .95 > 568.... 9.15 24 298 .94> 79.96 25 298.94 > 98.95 24 398 .84 > 79.96 25 3 9 8 .84> 98.96 7.46 24 398 .84> 118.70 22 498 .93> 79.96 8.29 23 4 98 .93> 98.96 8.29 31 498 > 78 10.05 21 4 1 4 .9 7 > 3 6 9 .... 7 .0 9 21 4 2 0 .9 7 > 3 7 5 .... 7 .0 9 416 .97 > 3 7 1 .9 7.09 21 50 3 > 79.96 8.29 21 5 0 3 > 9 8 .9 6 8.29 21 4 0 2 .9 > 8 4 7.33 21 4 0 2 .9 > 1 0 3 7.29 21 2 1 6 .9 9 > 1 7 1 .9 8 5 .1 7 21 3 1 4 .9 7 > 2 6 9 .9 8 6 .3 4 21 4 6 7 .9 6 > 4 2 2 .9 7 7 .49 21 5 14 .96 >46 9.9 6 7.99 21 6 14 .95 > 5 6 9 .... 9.15 21 5 0 6 > 7 8 9.96 458 .98 >393.98 7.38 A re a 349 772 723 154 493 9072 9625 6963 19 14184 11887 78 188317 66776 75036 8892 7623 8474 5852 23871 63063 70712 82665 140757 9536 29240 IS A rea 23871 63063 63063 63063 188317 188317 70712 82665 82665 140757 8474 5852 8474 5852 8474 8892 7623 9536 75036 75036 75036 75036 75036 75036 75036 75036 75036 75036 75036 75036 ng/g 0 .0 1 2 0 .0 8 3 0 .8 3 9 0 .8 2 0 0 .3 1 4 22.018 2 5 .6 8 7 38.953 7 .0 5 4 4 .7 9 4 18.357 19.303 17.828 18.511 15.384 17.003 14.195 12.414 12.808 9.269 5.120 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0 .1 3 6 6 0 .0 6 3 5 0 .0 3 9 6 0 .0 1 4 6 0 .0 6 7 7 0 .2 5 6 2 0 .0 7 6 0 0 .2 2 9 3 0 .0 2 7 7 0 .0 5 7 6 0 .0 4 8 6 0 .0 8 1 0 0 .0 7 2 9 0 .0 1 7 9 % Rec S /N 6 11 3 2 2 102 182 113 1 1 0 9 .3 1 1 8 .7 80.7 1 0 3 .0 1 0 8 .3 1 0 0 .0 1 0 3 .9 1 2 9 .5 143.1 1 1 9 .5 1 0 4 .5 1 0 7 .8 78.0 1 0 3 .4 288 509 15 1840 527 984 784 210 674 0 199 2094 750 713 522 508 858 N oise 232 280 268 137 837 295 730 316 236 272 31 33 117 63 5 174 89 27 404 493 301 43 133 48 0 468 135 378 426 1171 99 128 H e ight 1456 3049 2910 451 1692 32245 42946 30805 69 50045 45058 418 743648 259920 296161 33822 27937 32041 23858 92992 282955 283341 303512 611461 50076 109965 IS H e ig h t S a m p le size 92992 2.0200 282955 2 .0 2 0 0 282955 2.0200 282955 2.0200 743648 2.0200 743648 2.0200 283341 2.0200 303512 2 .0 2 0 0 303512 2.0200 611461 2.0200 32041 2.0200 23858 2.0200 32041 2.0200 23858 2 .0 2 0 0 32041 2 .0 2 0 0 33822 2 .0 2 0 0 27937 2 .0 2 0 0 50076 2 .0 2 0 0 296161 2 .0 2 0 0 296161 2 .0 2 0 0 2 .0 2 0 0 296161 2.0200 296161 2.0200 296161 2 .0 2 0 0 296161 2 .0 2 0 0 296161 2.0200 296161 2 .0 2 0 0 296161 2 .0 2 0 0 296161 2 .0 2 0 0 296161 2 .0 2 0 0 296161 2 .0 2 0 0 2 .0 2 0 0 F actor2 D e tectio n... 1.0 M MI 1.0 1.0 bbl 1.0 1.0 M M 1.0 M M 1.0 bbl 1.0 bb 1.0 M M 1.0 M M 1.0 1.0 1.0 1.0 M M I 1.0 1.0 bb 1.0 bb 1.0 M M I 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 118 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-11,, Name: FC3G_013S031 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:58:51 PFBA FC3GJD13S031 100- 5 .1 7 ^ 6 .1 0 . 25 J 6 49 4.05 r aM > 5.00 ( ......... min 6.00 7.00 PFPeA FC3G 013S031 100-^ 5 .9 5 ;1 .7 3 e 2 ;7 8 5 5.71. i . 5.11 J ! 1 6.83 tyY%: 4.4 4 I J J i if w * V Mi.ni.iiim min 5.00 6.00 7.00 PFHxA FC3G__013S031 100TM, - 6.31 6.61 e2 i 3714 | r % : 4 .0 7 6.10r1li 0i ( /I V -J v ir''"' / 5.00 6.00 7.00 PFNA FC3G 013S031 PFDA FC3G_013S031 100 7.9 9 8 .1 0e3 30718 PFUnA FC3G 013S031 PFHxS-1 FC3G_013S031 7.00 8.00 8.00 10.00 12.00 PFHxS-2 FC3G_013S031 PFHxS-3 FC3G_013S031 100TM 7.46 7.78 / AA A A 8.02 % - 7.09 / i, w V \' ' '/I ' ' ' ' I ' \ 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S031 PFOA-1 FC3G 013S031 PFOA-2 FC3G 013S031 7.00 8.00 PFDoA FC3G 013S031 PFBS-1 FC3G 013S031 5.00 6.00 7.00 PFBS-2 FC3G_013S031 100- 4 .0 3 7.11 \* I ftill* 6.63 , Il I^j!f, \ y 5.00 6.00 7.00 PFOS-1 FC3G_013S031 100- 8.29 F 2.42e4 1 87760 1 1 ,, ................. . , min 8.00 10.00 12.00 PFOS-2 FC3G 013S031 100- 8.29 2 .0 3 e 4 1 78391 (8 %- 1 II 0 - - _ - ,, , . ITTTT. , , rrTT min 8.00 10.00 12.00 PFOSA FC3G_013S031 9.96 100-, 1.57e2 I : 886 I %- I 7.81 I 10.53 0^ I'ru m if l 8.00 10.00 12.00 m Page 119 of 234 .axysanalytical.com Q uantify Sam ple R eport M a s s L y n x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld LastAltered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-11,, Name: FC3G_013S031 D escription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 20:58:51 13C2-PFOA FC3GJD13S031 100- 7.09 i 1.46e5 591076 % 13C8-PFOA FC3G_013S031 100--i 7.09 5.36e4 ! 208095 0 7.00 8.00 1802-PFHxS-2 FC3G 013S031 100- 7.29 4 5.12e3 >' 19704 %- 7.00 8.00 13C4-PFBA FC3G 013S031 100- 5.17 2.53e4 1 84917 %4.18 7.00 8.00 5.00 6.00 7.00 13C8-PFOSA FC3G 013S031 100- 9.96 7.53e3 44921 I%: o- ............ 4Mrn-TTT-rrrT min 8.00 10.00 12.00 13C2-PFOUEA FC3G 013S031 100-- 7.38 2.74e4 106373, " 07.00 lilw 8.00 13C4-PFOA FC3G_013S031 100- 7.09 f 5.6504 l | 227290 %- 7.00 8.00 13C2-PFHxA FC3G_013S031 100^ 6.33 5.23e4 250761 5.00 6.00 7.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S031 13C4-PFOS-2 FC3G 013S031 100- 8.29 F 6.35e3 I 2464 %- 1802-PFHxS-1 FC3G 013S031 7.29 100 j 7.36e3 / ; 30290 %- 0 - min 8.00 10.00 12.00 0 7.00 8.00 13C5-PFNA FC3G 013S031 100- 7.46 5.76e4 J 238472 1 %- 0- i llill min 7.00 8.00 13C2-PFDA FC3G 013S031 100 7.99 i 6.85e4 !, 246337 %M 0 - min 8.00 10.00 12.00 13C2-PFDOA FC3G 013S031 100 9.15 9.98e4 436770 %: 0; 8.00 10.00 12.00 m Page 120 of 234 www.axysanalytical.com Q uantify Sam ple Report M a ssL yn x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qid Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-11,, Name: FC3G_013S031 D escription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 20:58:51 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 1302-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 1302-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 27 262.98 >218.... 5.95 27 312.97> 268.98 6.31 27 362.97> 318.98 19 412.97> 368.98 7.05 19 412.97> 168.80 7.14 28 462.96 >418.... 7.49 29 512.96 >468.... 7.99 29 562.96 >518.... 8.55 30 612.95 >568.... 9.15 24 298.94> 79.96 25 298.94 > 98.95 24 398.84 > 79.96 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.96 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.17 21 314.97>269.98 6.33 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 173 661 422 113 259 8104 7216 4805 24248 20335 157 145941 53626 56505 7457 6347 7358 5121 25274 52259 57617 68523 99834 7529 27351 IS Area 25274 52259 52259 52259 145941 145941 57617 68523 68523 99834 7358 5121 7358 5121 7358 7457 6347 7529 56505 56505 56505 56505 56505 56505 56505 56505 56505 56505 56505 56505 ng/g 0.034 0.074 0.895 0.710 0.287 43.915 51.732 0.066 38.931 7.306 3.506 19.854 20.727 19.962 20.891 21.005 18.171 14.916 13.271 11.715 9.439 4.651 PV by LHE, SV by KJC 31-Jan-13. Note: Caiver = ng abs. SPM = ng/mL. LOD 0.0247 0.2115 0.0028 0.0229 0.0435 0.1822 0.2201 0.0502 0.1601 1.8124 0.0502 0.0244 0.0401 0.0535 0.1077 0.0112 %Rec S/N 3 17 3 1 2 150 102 118 112.5 126.6 60.8 114.7 119.8 115.3 120.7 182.0 157.5 129.3 115.0 101.5 81.8 96.7 997 453 22 , 41912 922 242 309 273 1221 374 29 1296 1888 888 714 390 1244 Noise 291 226 215 253 405 344 577 205 295 190 34 25 266 49 52 88 173 41 14 226 940 92 90 25 53 2924 194 126 277 611 115 86 Height 785 3714 1314 426 1041 30718 30117 22501 87760 78391 886 591076 208095 227290 28384 24640 30290 19704 84917 250761 238472 246337 436770 44921 106373 IS Height 84917 250761 250761 250761 591076 591076 238472 246337 246337 436770 30290 19704 30290 19704 30290 28384 24640 44921 227290 227290 227290 227290 227290 227290 227290 227290 227290 227290 227290 227290 Sample size 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 2.0800 Factor2 Detection... 1.0 1.0 bb 1.0 MMI 1.0 1.0 MMI 1.0 MM 1.0 bbl 1.0 MM 1.0 MM 1.0 MM 1.0 1.0 1.0 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 121 of 234 www.axysanalytical.com Q uantify Sam ple R eport M a ssL yn x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-12,, Name: FC3G_013S032 D escription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 21:15:52 PFBA FC3G_013S032 1006.18 4.20 i V 6-5^ : 21 :V j j 0- 5.00 6.00 7.00 PFPeA FC3G_013S032 100- 25.0848e2~], 6.73 913 /i 6.92 k ij'ii'i 'V -in v 5.00 6.00 7.00 PFHxA FC3G_013S032 100- 6.29 8.34c2 I 3779 i %- 6.10 4.57 .vWV\ jj E^ 5.00 6.00 7.00 PFNA FC3G 013S032 100- 7.55 5.86e2 - 1818 . %: 6.63 W .05; 0J 7.00 -T min 8.00 PFDA FC3G 013S032 100- r 7.99 5.21 e3 _ jj 18690 %- 0: i L . 11A7 , t min 8.00 10.00 12.00 PFUnA FC3G 013S032 100- 8.52 S~4.82e3 : |i 19155 %z 0- 10 8 6 . 8.00 10.00 12.00 PFHxS-1 FC3G 013S032 PFHxS-2 FC3G_013S032 PFHxS-3 FC3G_013S032 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S032 Tn- min PFOA-1 FC3G__013S032 100- 7.09 |T7.41e2 I I 2188 %- 8.20 7.00 8.00 PFOA-2 FC3G 013S032 PFDoA FC3G_013S032 100- 9.12 f 3.59e3 1 15320 %- PFBS-1 FC3G 013S032 PFBS-2 FC3G 013S032 , . 1100;.59 8.00 10.00 12.00 PFOS-1 FC3G 013S032 100 8.29 F 3.45e3 I 12665 %I o : min 8.00 10.00 12.00 PFOS-2 FC3G 013S032 100- 8.29 2.76e3 I1 10399 %- i m km 0- 8.00 10.00 min 12.00 PFOSA FC3G 013S032 100- 9.93 9.83e1~1 568 %7.70 / 08.00 10.74 '/ 10.00 12.00 m Page 122 of 234 www.axysanalytical.com Q ua ntify Sam ple R eport M a ssL yn x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G 013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-12,, Name: FC3G_013S032 D escription: 1,W G42409,15/4000uL D ate: 11 J a n -2 0 1 3 Tim e: 21:15:52 13C2-PFOA FC3G013S032 100-j 7.09 2.04e5 810887 13C8-PFOA FC3G 013S032 100- 7.09 IT 7.79o4 296062 %- -T-r min qJ 7.00 8.00 7.00 8.00 18 0 2 -P F H xS -2 FC3G_013S032 100- 7.29 i 7.05e3 30047 %- 13C4-PFBA FC3G 013S032 13C4-PFOA FC3G_013S032 100- 7.05 I" 8.02e4 314777 %- 7.00 8.00 13C2-PFHxA FC3G 013S032 7.00 8.00 13C8-PFOSA FC3G_013S032 100- 9.96 6.43e3 40418 ; o - , _____ 4 min 8.00 10.00 12.00 13C2-PFOUEA FC3G_013S032 100n 7.33 f 2.97e4 (109881 %- 0-* 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S032 100- 8.29 s 1.06e4 : 40587 % 8.00 10.00 12.00 13C5-PFNA FC3G_013S032 100- 7.46 7.09e4 ~k 292943 ; \ %- 7.00 8.00 13C4-PFOS-2 FC3G 013S032 100- 8.29 F"6.67e3 1 23094 1802-PFHXS-1 FC3G 013S032 100- 7.29 A 9.11e3 ; \ 35468 %" I I 8.00 10.00 12.00 - ; JSlilihsa_ 7.00 8.00 13C2-PFDA FC3G 013S032 100- 7.99 f 9.12e4 336205 %1a o- 4 r X . ....... min 8.00 10.00 12.00 13C2-PFDOA FC3G_013S032 100- 9.15 1.17e5 S' %- :\ (H ------8.00 10.00 12.00 Page 123 of 234 www.axysanalytical.com Q uantify Sam ple Report M a ssL yn x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-12,, Name: FC3G_013S032 D e scription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 21:15:52 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.17 27 262.98 >218.... 5.88 27 312.97> 268.98 6.29 27 362.97> 318.98 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.55 29 512.96 >468.... 7.99 29 562.96 > 518.... 8.52 30 612.95 >568.... 9.12 24 298.94> 79.96 25 298.94 > 98.95 24 398.84 > 79.96 7.33 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.93 21 414.97 >369.... 7.09 21 420.97 >375.... 7.09 416.97 >371.9 7.05 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.21 21 314.97>269.98 6.33 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.96 458.98 >393.98 7.33 Area 754 204 834 741 234 586 5205 4819 . 3590 122 3451 2760 98 204485 77907 80215 10580 6666 9114 7053 25466 62831 70871 91217 116772 6429 29725 IS Area 25466 62831 62831 62831 204485 204485 70871 91217 91217 116772 9114 7053 9114 7053 9114 10580 6666 6429 80215 80215 80215 80215 80215 80215 80215 80215 80215 80215 80215 80215 ng/g 0.033 0.004 0.127 0.006 0.315 0.300 0.060 0.150 4.123 6.292 0.007 38.425 7.477 4.978 19.842 15.335 17.419 20.267 14.909 15.390 12.924 12.444 9.653 5.677 5.055 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0085 0.0312 0.0242 0.0040 0.0231 0.0828 0.3226 0.1145 0.2280 0.3046 0.0222 0.0115 0.0222 0.0337 0.0301 0.0036 %Rec S/N 7 6 12 5 0 4 108 103 126 8 111.0 129.6 86.3 114.6 88.6 100.6 117.1 129.2 133.4 112.0 107.9 83.7 49.2 105.1 179 98 20 4823 5374 645 703 126 453 289 147 2385 3563 1580 929 907 4216 Noise 322 159 315 263 450 0 493 174 186 122 32 19 50 0 44 71 107 29 168 55 488 58 183 78 104 681 119 82 213 533 45 26 Height 2352 913 3779 2188 770 1818 18690 19155 15320 403 12665 10399 568 810887 296062 314777 40587 23094 35468 30047 100126 282673 292943 336205 494743 40418 109881 IS Height Sample size 100126 2.0800 282673 2.0800 282673 2.0800 282673 2.0800 810887 2.0800 810887 2.0800 292943 2.0800 336205 2.0800 336205 2.0800 494743 2.0800 35468 2.0800 30047 2.0800 35468 2.0800 30047 2.0800 35468 2.0800 40587 2.0800 23094 2.0800 40418 2.0800 314777 2.0800 314777 2.0800 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 2.0800 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 MMI 1.0 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 1.0 1.0 MM 1.0 1.0 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 124 of 234 .axysanalytical.com Q uantify Sam ple R eport M a ssL yn x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-13,, Name: FC3G_013S033 D e scription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 21:32:45 PFBA FC3G 013S033 PFPeA FC3G013S033 100-, 0/1 5.99 6.77e2 3444 6.57 5.77 1 f 7.16 : 4.095.51 if y Vv 1 0--5-ti-H 11.1111--;---- - |TTT min 5.00 6.00 7.00 PFHxA FC3G013S033 100- 7.21 A6.34 1.20e3 ], 6266 4.09 5.92 k ; 5.00 6.00 7.00 PFNA FC3G 013S033 PFDA FC3G_013S033 100- _ 7.99 3.30e3 i 10156 %H f 11.99 ilKn /v. a a-.A--. -vV/" o 8.00 10.00 12.00 PFUnA FC3G_013S033 PFHxS-1 FC3G 013S033 PFHxS-2 FC3G_013S033 PFHxS-3 FC3G_013S033 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S033 PFDoA FC3G013S033 100- 9.15 F 2.71e3 I 11059 % 11.99 8.00 10.00 12.00 PFOS-1 FC3G 013S033 PFOA-1 FC3G 013S033 100" 7.09 JTl.22e3 / \ 4707 %: -- v 7M 07.00 8.00 PFBS-1 FC3G_013S033 PFOS-2 FC3G 013S033 PFOA-2 FC3G 013S033 100- 7.55 1.31e3 4908 %- ^ \ ^7.14 / 0- __ 7.00 8.00 PFBS-2 FC3G 013S033 PFOSA FC3G 013S033 Page 125 of 234 www.axysanalytical.com Q uantify Sam ple R eport M a s s L y n x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-13,, Name: FC3G_013S033 D e scription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 21:32:45 13C2-PFOA FC3G_013S033 100 2.04e5 .795762 oo 13C8-PFOA FC3G 013S033 ^ 8.67e 4 338988 %{ 13C4-PFOA FC3G013S033 100-n 7.09 . 8.61e4 339908 %: 7.00 I - n min 8.00 07.00 t min 8.00 o: -liR i 7.00 8.00 18 0 2 -P F H x S -2 FC3G_013S033 7 IQ 100- ]6 .2 4 e 3 2544-i . 7.00 T - min 8.00 13C4-PFBA FC3G 013S033 100%- 2.90e4 1 96551 y 4.13 / 0 WSWrfmTTTmTTVT min 5.00 6.00 7.00 13C2-PFHxA FC3G 013S033 100- 6 .2 1 e 4 l 305628 %- o - Ur,T,.... ,.ifc 5.00 6.00 7.00 13C8-PFOSA FC3G 100- 013S033 9.99 9 .4 5 e 3 l 50155 J o o 13C2-PFOUEA FC3G013S033 7.43 3.92e4 , - 147311 0- I i 1 min 8.00 10.00 12.00 o7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S033 100- 8.29 8.53e3 29933 0 I j^'TnyrrTT]' ; i . ! i i i i i r 8.00 10.00 12.00 13C5-PFNA FC3G 013S033 13C4-PFOS-2 FC3G013S033 100-, 8.29 f 7.43e3 I 27026 %- 8.00 10.00 12.00 13C2-PFDA FC3G_013S033 100- 7.99 F 8.70e4 I 314678 %- 8.00 10.00 12.00 18 02 -P F H xS -1 FC3G_013S033 100 - 7.33 9.54e 3l " 38038 I %- . ...,- r 7.00 8.00 13 C 2 -P F D oA FC3G_013S033 100 9.15 F 1.51e5 I 647094 % O-Vt 8.00 10.00 12.00 m Page 126 of 234 www.axysanalytical.com Q ua ntify Sam ple R eport M a ssL yn x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-13,, Name: FC3G_013S033 D escription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 21:32:45 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.23 27 262.98 >218.... 5.99 27 312.97> 268.98 6.34 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 28 462.96 >418.... 7.49 29 512.96 >468.... 7.99 29 562.96 >518.... 8.55 30 612.95 > 568.... 9.15 24 298.94> 79.96 25 298.94 > 98.95 24 398.84 > 79.96 25 398.84> 98.96 7.29 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.99 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171,98 5.19 21 314.97>269.98 6.36 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.99 458.98 >393.98 7.43 Area 759 677 1201 746 1216 1743 3301 4583 2706 42 4021 2951 197 203619 86741 86068 8526 7429 9537 6241 29036 62135 70010 87044 150877 9451 39188 IS Area 29036 62135 62135 62135 203619 203619 70010 87044 87044 150877 9537 6241 9537 6241 9537 8526 7429 9451 86068 86068 86068 86068 86068 86068 86068 86068 86068 86068 86068 86068 ng/g 0.146 0.025 0.075 0.163 0.125 0.279 5.717 5.638 0.063 33.411 7.269 5.004 13.962 14.922 15.916 15.661 14.843 13.289 11.148 10.370 10.891 7.288 6.243 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0160 0.0317 0.0061 0.0352 0.0940 0.0979 0.0200 0.1760 0.6860 0.0438 0.0334 0.0218 0.0410 0.0331 0.0533 %Rec S/N 4 15 21 13 25 10 50 50 55 1 103.0 134.5 92.6 86.1 92.0 98.2 96.6 137.3 122.9 103.1 95.9 100.7 67.4 138.6 107 99 57 3133 3548 427 396 421 2413 276 55 1135 999 1307 865 888 351 Noise 697 227 305 257 188 548 701 202 348 200 27 22 109 114 87 127 109 19 254 96 797 76 64 16 92 1766 269 276 241 748 56 419 Height 2764 3444 6266 3444 4707 6738 10156 17414 11059 149 13539 10805 1094 795762 338988 339908 29933 27026 38038 25444 96551 305628 275578 314678 647094 50155 147311 IS Height Sample size 96551 2.2200 305628 2.2200 305628 2.2200 305628 2.2200 795762 2.2200 795762 2.2200 275578 2.2200 314678 2.2200 314678 2.2200 647094 2.2200 38038 2.2200 25444 2.2200 38038 2.2200 25444 2.2200 38038 2.2200 29933 2.2200 27026 2.2200 50155 2.2200 339908 2.2200 339908 2.2200 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 2.2200 Factor2 Detection. 1.0 MMI 1.0 MM 1.0 MMI 1.0 bb 1.0 MM 1.0 MM-I 1.0 MM 1.0 MM 1.0 MM 1.0 MMI 1.0 1.0 1.0 1.0 MMI 1.0 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 127 of 234 .axysanalytical.com Q uantify Sam ple R eport M a ssL yn x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-14,, Name: FC3G_013S034 D escriptio n: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 21:49:46 PFBA FC3G 013S034 PFPeA FC3G_013S034 PFHxA FC3G 013S034 PFNA FC3G_013S034 7 AG 100 - 7.11e2~". 2429 -6 .6 3 7.00 m in 8.00 PFDA FC3G 013S034 1001 1.19e4 41075 i, % - i! 0- J m in 8.00 10.00 12.00 PFUnA FC3G_013S034 loo-q 8.52 fl.5 6 e 4 I 68837 %" - 0 -1 8.00 10.00 12.00 PFHxS-1 FC3G 013S034 PFHxS-2 FC3G013S034 PFHxS-3 FC3G 013S034 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S034 PFDoA FC3G 013S034 PFOA-1 FC3G 013S034 PFBS-1 FC3G 013S034 PFOA-2 FC3G 013S034 100- 7.49 7.09 A 2.94e2 I \ % - 1452 j \ A I\, .J s :, 0- 7.00 8.00 PFBS-2 FC3G 013S034 PFOS-1 FC3G 013S034 P F O S -2 FC3G_013S034 PFOSA FC3G_013S034 100 9.96 1.73e3l 9243 %- -8.00 10.00 12.00 1 C Page 128 of 234 .axysanalytical.com Q uantify Sam ple R eport M a ssL yn x 4.1 SCN627 D ataset: G :\M asslynx\lnstJ3\PFC .PRO \FC \FC3G 013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-14,, Name: FC3G_013S034 D escription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 21:49:46 13C2-PFOA FC3G_013S034 100- 7.09 j r 2.00e5 *1794915 % 13C8-PFOA FC3G_013S034 100- 7.09 C 8.26e4 ,323223 7.00 8.00 18 02 -P F H xS -2 FC3G_013S034 100- 7.33 _ G.53e3, 237151 %- 7.00 8.00 13C8-PFOSA FC3G_013S034 100- 9.96 1.13e4 j 61673 %- JllbJ 8.00 10.00 12.00 7.00 8.00 13C4-PFBA FC3G_013S034 100- 5.21 3.08e4 A 103299 %- 4.03 5.00 6.00 7.00 13C2-PFOUEA FC3G_013S034 100-- 7.38 3.4304 .. "127907 H %- 7.00 8.00 13C4-PFOA FC3G__013S034 100- 7.09 if" 8.17e4 i \ 323569 %- 0-M*, | i i r-rlvTi . . i i min 7.00 8.00 13C2-PFHxA FC3G 013S034 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S034 100- 8.29 9.68e3 ! 36627 %- 13C4-PFOS-2 FC3G 013S034 100- 8.29 j 7.60e3 1 29426 %-- 1802-PFHXS-1 FC3G013S034 100-, 7.29 i 9.55o3 : i l 37380 %- V o - .............. min 8.00 10.00 12.00 0 - iJllikL min 8.00 10.00 12.00 (H 7.00 8.00 13C5-PFNA FC3G_013S034 7 Aa 100 ] 7.81 e4~l 308838 I %- , / # % r r i min 7.00 8.00 13C2-PFDA FC3G 013S034 100- r 8.81 e4 S 331279 % i ;; o: ! ' min 8.00 10.00 12.00 13C2-PFDoA FC3G_013S034 1 0 0 -, 9.15 1.65e5 719824 %- 0-* 12.00 Page 129 of 234 www.axysanalytical.com Q uantify Sam ple R eport M a ssL yn x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-14,, Name: FC3G_013S034 D escriptio n: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 21:49:46 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.28 27 262.98 > 218.... 5.93 27 312.97> 268.98 6.38 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 > 418.... 7.49 29 512.96 >468.... 7.99 29 562.96 > 518.... 8.52 30 612.95 >568.... 9.15 24 298.94> 79.96 6.49 25 298.94 > 98.95 24 398.84 > 79.96 7.29 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79,96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.96 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.33 21 216.99>171.98 5.21 21 314.97>269.98 6.33 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 373 276 1141 310 1283 294 711 11877 15601 13836 25 217 63604 50518 1728 200132 82573 81741 9676 7598 9545 6527 30842 70376 78108 88052 165435 11302 34271 IS Area 30842 70376 70376 70376 200132 200132 78108 88052 88052 165435 9545 6527 9545 6527 9545 9676 7598 11302 81741 81741 81741 81741 81741 81741 81741 81741 81741 81741 81741 81741 ng/g 0.046 0.097 0.178 0.015 1.084 1.309 0.773 0.281 91.742 111.053 1.525 38.001 8.008 5.223 18.337 17.660 18.435 18.954 18.245 17.418 14.393 12.139 13.819 10.085 6.001 LOO 0.0049 0.1764 0.0843 0.0241 0.0217 0.0879 0.0740 0.0668 0.0425 0.1364 0.0347 0.0193 0.0340 0.0472 0.0154 0.0090 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec S/N 7 6 18 3 32 27 7 149 401 330 7 4 106.6 134.8 87.9 102.9 99.1 103,4 106.4 153.6 146.6 121.1 102.2 116.3 84.9 121.2 2311 1307 281 1358 986 721 599 701 819 1228 340 1743 2237 1018 965 2708 2007 Noise 214 157 238 361 142 53 338 276 172 187 24 38 186 0 92 98 141 33 586 328 449 61 42 46 19 304 185 138 326 746 23 64 Height 1455 973 4242 1157 4614 1452 2429 41075 68837 61824 162 723 225572 183992 9243 794915 323223 323569 36627 29426 37380 23715 103299 322463 308838 331279 719824 61673 127907 IS Height Sample size 103299 2.0200 322463 2.0200 322463 2.0200 322463 2.0200 794915 2.0200 794915 2.0200 308838 2.0200 331279 2.0200 331279 2.0200 719824 2.0200 37380 2.0200 23715 37380 2.0200 2.0200 23715 2.0200 37380 2.0200 36627 2.0200 29426 2.0200 61673 2.0200 323569 2.0200 323569 2.0200 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 323569 2.0200 2.0200 323569 323569 2.0200 2.0200 2.0200 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 MMI 1.0 MMI 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MMI 1.0 1.0 MM 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 130 of 234 www.axysanalytical.com Q ua ntify Sam ple R eport M a ssL yn x 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: W G 42409-103,,DUP Name: FC3G_013S035 D e scription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 22:06:47 PFBA FC3G_013S035 100- i %- 4.18 6-1.6.33 ,!ML-6.53 V'* min 5.00 6.00 7.00 PFPeA FC3G 013S035 100- 5-99 6.59 7 .2 0 e ll f\ 6" f j \ %- \ f X 3 5 s V 0-l" " t " " iii"i-'"i))iT mr rT min 5.00 6.00 7.00 PFHxA FC3G013S035 100-> 6.34 9.60e2l 4945 7.26 %- / 5.19 6' 12f \ -Jm ' 0- 5.00 6.00 7.00 PFNA FC3G_013S035 7 yio 100 6.13e2 \ 1937 7.00 -K - -r min 7.00 8.00 PFQA FC3G_013S035 100- f 1.31e4 [i 48883 %- !! 0^ r r _ r r ttt min 8.00 10.00 12.00 PFUnA FC3G__013S035 100-1 8.52 1.54e4 68198 %: - 8.00 10.00 12.00 PFHxS-1 FC3G 013S035 PFHxS-2 FC3G 013S035 PFHxS-3 FC3G 013S035 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G013S035 R1 100 7.48e2 2929 : ; 4.03 J.l J i...... ' .... ...... ..... min 5.00 6.00 7.00 PFOA-1 FC3G 013S035 100- %- / V7.55 ............\ 07.00 8.00 min PFOA-2 FC3G013S035 100- 7.55 %J o7.00 8.00 PFDoA FC3GJ513S035 Q 100- 1.41e4 i 62406 ) i; -- -Tr min 8.00 1C.00 12.00 PFBS-1 FC3G 013S035 100- , 6.68 5.95 In 5.06 %4.48 I I SIli I 0 - \ i : K f mmiinn 5.00 6.00 7.00 PFBS-2 FC3G_013S035 6.44 ioo-q 6.44 2.72e1 2 . 7 2 e ll 225 225 1 / % - 6.25 II 6.68 4.70 kk lili o- L 5.00 6.00 7.00 PFOS-1 FC3G013S035 100 8.29 i 6.20e4 219219 % PFOS-2 FC3G 013S035 PFOSA FC3G_013S035 100-1 9.96 1.97e3 10371 % 8.00 10.00 12.00 0~Vt i... 8.00 10.00 12.00 m P age 131 o f 234 .axysanalytical.com Quantify Sam ple Report M a ssLy n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: W G42409-103,,DU P Name: FC3G_013S035 Description: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 22:06:47 1 3 C 2 -P F O A FC3G 013S035 100- 7.09 1.91e5 729114 %- 1 3 C 8 -P FO A FC3G013S035 100- 7.09 6.93e4 269858 %- 13 C 4 -P FO A FC3G_013S035 100q 7.09 { 7.68e4 1303316 %- 07.00 T^T-rn min 8.00 0: 7.00 -T-r min 8.00 07.00 8.00 1 8 0 2 -P FH X S -2 FC3G_013S035 100n 1 7.29 fi.650.3 I ] 26546 0//o_ 13C4-PFBA FC3G_013S035 100- 5.17 f 2.57e4 1 97083 %- 4.03 1 3 C 2 -P F H x A FC3G_013S035 100- 6.34 6.78e4 315223 %- 7.00 8.00 1 3C 8-P FO SA FC3G 013S035 5.00 6.00 7.00 1 3 C 2 -P FO U E A FC3G_013S035 100-- 7.38 3.22e4 I I :1220091 %- 5.00 6.00 7.00 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Caiver = ng abs. SPM = ng/mL. 1 3 C 4-P FO S-1 FC3G 013S035 1 3 C 4 -P F O S-2 FC3G 013S035 1 8 0 2 -P FH xS -1 FG3G 013S035 1 3 C 5 -P FN A FC3G_013S035 100- 7.49 7.01e4 ft 274346 1 %- I .J B . 7.00 8.00 1 3 C 2 -P F D A FC3GJD13S035 100- 7.99 F 8.36e4 I 300934 %1 0 , min 8.00 10.00 12.00 1 3 C 2 -P F D o A FC3G_013S035 m Page 132 of 234 www.axysanalytical.com Quantify Sam ple Report M a s sL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: W G42409-103,,DUP Name: FC3G_013S035 Description: 1,W G42409,15/4000uL Date: 11>Jan-2013 Tim e: 22:06:47 # Name ::Ty 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-l 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA ; T: ts# Trace RT 26 212.99>168.98 5.17 27 262.98 > 218. .. 5.99 27 312.97> 268.98 6.34 27 362.97> 318.98 6.68 19 412.97> 368.98 7.05 19 412.97> 168.80 7.09 28 462.96 >418.... 7.49 29 512.96 >468.... 7.99 29 562.96 >518.... 8.52 30 612.95 >568.... 9.12 24 298.94> 79.96 25 298.94 > 98.95 6.44 24 398.84 > 79.96 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.96 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.17 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 >569.... 9.12 21 506>78 9.96 458.98 >393.98 7.38 Area 691 72 960 748 582 98 613 13087 15446 14132 27 61996 48495 1969 190874 69322 76841 7662 7252 9957 6653 25742 67841 70055 83649 142202 10768 32202 IS Area 25742 67841 67841 67841 190874 190874 70055 83649 83649 142202 9957 6653 9957 6653 9957 7662 7252 10768 76841 76841 76841 76841 76841 76841 76841 76841 76841 76841 76841 76841 ng/g 0.017 0.034 0.010 1.178 1.246 0.896 102.826 101.620 1.688 35.081 6.507 4.467 14.054 16.317 18.614 18.700 14.740 16.252 12.495 11.162 11.497 9.300 5.130 LOD 0.2817 0.1109 0.0587 0.0167 0.0691 0.0531 0.1016 0.0777 0.0214 0.2798 0.0473 0.0781 0.0128 0.0475 0.0447 0.0072 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec S/N 9 5 18 10 17 1 6 191 200 178 9 108.2 120.4 82.6 86.7 100.6 114,8 115.3 136.3 150.3 115.6 103.2 106.3 86.0 113.9 766 706 302 1734 1156 194 709 448 737 2652 151 1214 476 2380 807 867 2127 Noise 287 133 279 306 91 577 334 256 342 351 53 25 296 114 38 286 242 34 421 233 1563 38 59 55 10 643 260 576 126 772 68 57 Height 2620 699 4945 2929 1534 483 1937 48883 68198 62406 225 219219 171040 10371 729114 269858 303316 27018 26607 40262 26546 97083 315223 274346 300934 623280 58997 122009 IS Height Sample size 97083 2.2200 315223 2.2200 315223 2.2200 315223 2.2200 729114 2.2200 729114 2.2200 274346 2.2200 300934 2.2200 300934 2.2200 623280 2.2200 40262 2.2200 26546 2.2200 40262 2.2200 26546 2.2200 40262 2.2200 27018 2.2200 26607 2.2200 58997 2.2200 303316 2.2200 303316 2.2200 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 2.2200 Factor2 Detection... 1.0 MMI 1.0 MMI 1.0 MMI 1.0 MM 1.0 bbl 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 1.0 MMI 1.0 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 133 of 234 www.axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-15,, Name: FC3G_013S036 D escription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 22:23:48 PFBA FC3G 013S036 PFPeA FC3G 013S036 100- 6.57 5.93 I V.44 5.00 f! PFHxA FC3G 013S036 PFNA FC3G_013S036 T AO 100 1 8.47e2 ;. 2814 - 7.00 ' ' ' I ' 1' 1' ' 1` 11 7.00 8.00 P F H xS -1 FC3G 013S036 5.00 6.00 7.00 PFDA FC3G 013S036 100- f f 1.68e4 ji 64032 %- ji _ !! 0 7i TTTn',rn r r ir r 8.00 10.00 12.00 P FH xS -2 FC3G 013S036 PFUnA FC3G_013S036 100q 8.52 ("9.11e3 1 37264 %: - 8.00 77-r-r min 10.00 12.00 P F H xS -3 FC3G_013S036 100- 7-24 %- :s,63 0 -^ 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S036 100- 6.49 6-73 \ f 3.30e2 411 5.52 6.01 | 1002 % - , ' U ,.A v A \'v - * 05.00 6.00 7.00 PFDoA FC3G 013S036 PFOA-1 FC3G 013S036 100- 7.09 & 6.69e2 i " 2550 : \ 7.67 /" 07.00 8.00 PFBS-1 FC3G 013S036 P FO A -2 FC3G_013S036 ioo-q 7.55 VA %- 07.00 8.00 P F B S -2 FC3G 013S036 P FO S-1 FC3G_013S036 DOQ 100- 5.46e4 194025 %- J l jl,,,,, min 8.00 10.00 12.00 P FO S-2 FC3G 013S036 100- f 4.23e4 153081 %- I 0J min 8.00 10.00 12.00 PFO SA FC3G 013S036 100- 8.98e2l 3917 j %- 8.00 10.00 12.00 m Page 134 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-15,, Name: FC3G_013S036 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:23:48 13C2-PFOA FC3G 013S036 13C8-PFOA FC3G013S036 100- 7.09 i 8.62e4 339609 %- 13C4-PFOA FC3G_013S036 100- 7.09 JT 8.85e4 I 347243 %- 7.00 8.00 0-%=*. 7.00 8.00 1802-PFHxS-2 FC3GJJ13S036 7' 100 1 |"7.11e3 1 129389 L - , . min 7.00 8.00 13C4-PFBA FC3G 013S036 100- 2.51 e4 ' 86623 %4.05 0J m min 5.00 6.00 7.00 13C2-PFHxA FC3G_013S036 100q 6.33 6.85e4 331308 %- . 0-1 5.00 6.00 7.00 13C8-PFOSA FC3G_013S036 100- 9.99 5.03e3l 23326 I %- 13C2-PFOUEA FC3G__013S036 100 7.38 -3-74e4 I "143278 i %- 8.00 10.00 12.00 tW, -- 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 1 3 C 4 -P FO S-1 FC3G 013S036 100- 8.29 T6.15e3 | 23651 ; %- 13C 4 -P FO S-2 FC3G_013S036 100-1 8.29 F 5.35e3 ~ I 19725 %- 0 - ____________ min 8.00 10.00 12.00 0- 8.00 10.00 12.00 1 3 C 5 -P F N A FC3G_013S036 100- 7.46 8 .0 4 e 4 _| 337018 i %- 1 3 C 2 -P F D A FC3G_013S036 100- f 7.99 8.42e4 S 328926 %- 7.00 8.00 Jr, 8.00 10.00 12.00 1 8 0 2 -P FH xS -1 FC3G013S036 100- 7.29 ;. 9.84e3 1 39297 % 7.00 8.00 1 3 C 2 -P F D o A FC3G_013S036 100- 9.12 6.17e4 265231 % l L mm 8.00 10.00 12.00 Page 135 of 234 www.axysanalytical.com Quantify Sam ple Report M a s sL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L1 9064-15,, Name: FC3G_013S036 Description: 1,W G42409,15/4000uL Date: 11 ^Jan-2013 Tim e: 22:23:48 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.21 27 262.98 >218.... 27 312.97> 268.98 6.33 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.05 28 462.96 >418.... 7.49 29 512.96 >468.... 7.99 29 562.96 >518.... 8.52 30 612.95 > 568.... 9.12 24 298.94> 79.96 6.49 25 298.94 > 98.95 6.47 24 398.84 > 79.96 7.29 25 398.84> 98.96 7.29 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.99 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.17 21 314.97>269.98 6.33 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 >569.... 9.12 21 506>78 9.99 458.98 >393.98 7.38 Area 460 1020 330 669 219 847 16777 9106 5604 64 25 157 112 54584 42348 898 226109 86209 88540 6150 5348 9843 7114 25057 68538 80398 84208 61724 5025 37391 IS Area 25057 68538 68538 68538 226109 226109 80398 84208 84208 61724 9843 7114 9843 7114 9843 6150 5348 5025 88540 88540 88540 88540 88540 88540 88540 88540 88540 88540 88540 88540 ng/g 0.084 0.024 1.378 0.606 0.700 0.025 0.153 99.784 106.506 1.457 31.899 6.212 4.553 8.659 9.236 14.123 15.348 11.013 12.603 11.007 8.625 3.831 3.332 5.269 LOD 0.0609 0.0106 0.0459 0.0007 0.0198 0.0317 0.0019 0.0283 0.0529 0.1387 0.0177 0.1390 0.0260 0.0257 0.0319 0.0328 0.0200 0.0049 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec S/N 8 15 5 6 3 11 276 116 206 8 10 8 4 111.2 129.9 95.2 60.4 64.4 98.5 107.0 115.2 131.8 115.1 90.2 40.1 34.8 132.2 1064 796 107 3035 10116 483 481 188 2440 0 210 1789 1375 808 384 592 3246 Noise 240 192 294 210 439 229 264 232 322 109 67 29 80 81 0 182 192 37 293 34 718 49 105 16 0 413 185 245 407 690 39 44 Height 1947 4288 1002 2550 773 2814 64032 37264 22536 569 294 659 354 194025 153081 3917 890381 339609 347243 23651 19725 39297 29389 86623 331308 337018 328926 265231 23326 143278 IS Height Sample size 86623 2.5100 331308 2.5100 331308 2.5100 331308 2.5100 890381 2.5100 890381 2.5100 337018 2.5100 328926 2.5100 328926 2.5100 265231 2.5100 39297 2.5100 29389 2.5100 39297 2.5100 29389 2.5100 39297 2.5100 23651 2.5100 19725 2.5100 23326 2.5100 347243 2.5100 347243 2.5100 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 2,5100 Factor2 Detection... 1.0 MMI 1.0 1.0 MMI 1.0 MMI 1.0 MMI 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MMI 1.0 MM 1.0 MMI 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb Page 136 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-16,, Name: FC3G_013S037 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:40:41 PFBA FC3G 013S037 100 l5.26;5.11e2;1990 1/ ; ' >6.31 :i 5.00 6.00 7.00 PFPeA FC3G_013S037 5 gQ 100" 1179 ] / %- 4.82 6.53 \ ki f r J H 11 5.00 6.00 7.00 PFHxA FC3G_013S037 100- 6.31 8.82e2 3664 %H4.03 r ' 5.90 5.00 6.00 7.00 PFNA FC3GJ513S037 100- 7.55 3.73e2l 1331 1 % 6.76 / 8.20 7.00 8.00 PFDA FC3G_013S037 100- r2.6799e93 I 1 8484 8.00 10.00 12.00 PFUnA FC3G_013S037 100- _ 8.55 2.73e3 10506 0A=--. . 10.98 8.00 10.00 12.00 PFHxS-1 FC3G 013S037 PFHxS-2 FC3G_013S037 PFHxS-3 FC3G 013S037 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S037 100- 4f 8 6.73 \o 3 5 80 . j ] 7.26 .7 h U v : U uMj r 0J r, 5.00 6.00 7.00 PFDoA FC3G 013S037 PFOA-1 FC3G_013S037 100 7.05 8.49e2 I 2690 7.00 8.00 PFBS-1 FC3G 013S037 P FO A -2 FC3G 013S037 P F B S -2 FC3G 013S037 P FO S-1 FC3G 013S037 P FO S-2 FC3G 013S037 PFO SA FC3G 013S037 )][(& Page 137 of 234 .axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L1 9064-16,, Name: FC3G_013S037 D escription: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 22:40:41 13C 2 -P FO A FC3G_013S037 100- 7.09 fl.98e5 757539 %l 13C 8 -P FO A FC3G_013S037 100n 7.09 7.41 e4 I 286801 %- 1 3 C 4 -P FO A FC3G..013S037 100- 7.09 I " 8.31e4 1320936 %- 0- 7.00 8.00 7.00 8.00 7.00 8.00 1 8 0 2 -P FH xS -2 FC3G_013S037 100-I I ^;.58e3 26847 i - T - . - . i min 7.00 8.00 13C4-PFBA FC3G 013S037 10 - 2.43e4 1 92954 %4.16 0 b ^ |T r^ T T 7mT-rr,-iiTrT min 5.00 6.00 7.00 1 3 C 2 -P F H x A FC3G_013S037 6.34 1001 6.03e4 ; 269675 %: 0^ I" | . 11" |. 5.00 6.00 7.00 13C8-PFO SA FC3G_013S037 100- 9.96 7.24e3l 44032 S %- 1 3 C 2 -P FO U E A FC3G_013S037 100-- 7.38 2.66e4 :102015/ \ %- 8.00 10.00 12.00 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 1 3 C 4 -P FO S-1 FC3G013S037 100- 8.29 r 9q.08e3 34951 8.00 10.00 12.00 1 3 C 5 -P FN A FC3G013S037 100- 7.46 7.17e4 . 289170 M %- 7.00 8.00 1 3 C 4 -P FO S-2 FC3G 013S037 100- 8.29 f 8.69e3 1 35006 1 8 0 2 -P FH xS -1 FC3G 013S037 100- \ 7.29 9.82e3 ; 40523 %- ' \ 8.00 10.00 12.00 rryv+ri-i "i . 7.00 8.00 1 3 C 2 -P F D A FC3G 013S037 100- f 7.99 7.60e4 274055 %: 1 3 C 2 -P F D o A FC3G 013S037 100- 9.15 f 1.25e5 543049 %- o: " min 8.00 10.00 12.00 0" i , 8.00 10.00 12.00 Page 138 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PR0\FC\FC3G..013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-16,, Name: FC3GJJ13S037 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:40:41 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 > 218. .. 5.90 27 312.97> 268.98 6.31 27 362.97> 318.98 19 412.97> 368.98 7.05 19 412.97> 168.80 28 462.96 >418.... 7.55 29 512.96 >468.... 7.99 29 562.96 >518.... 8.55 30 612.95 >568.... 9.15 24 298.94> 79.96 25 298.94 > 98.95 24 398.84 > 79.96 25 398.84> 98.96 7.33 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.90 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.19 21 314.97>269.98 6.34 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 511 238 882 849 373 2688 2726 3292 15 1295 957 66 197796 74067 83109 9083 8694 9824 6578 24324 60341 71728 75992 125157 7245 26583 IS Area 24324 60341 60341 60341 197796 197796 71728 75992 75992 125157 9824 6578 9824 6578 9824 9083 8694 7245 83109 83109 83109 83109 83109 83109 83109 83109 83109 83109 83109 83109 ng/g 0.045 0.026 0.112 0.170 0.002 1.657 1.368 36.577 6.995 5.258 16.763 19.682 18.477 18.602 14.014 14.545 12.872 10.202 10.181 6.296 4.609 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0158 0.0052 0.0016 0.0486 0.0693 0.1933 0.0898 0.2814 0.0328 0.0041 0.0198 0.0475 0.0392 0.0109 %Rec S/N 6 6 16 8 5 39 45 77 3 103.6 118.9 89.4 95.0 111.5 104.7 105.4 119.1 123.6 109.4 86.7 86.5 53.5 94.0 47 32 24 20870 13288 325 723 318 659 0 148 1541 9793 1442 723 759 1273 Noise 353 192 224 164 319 81 244 215 235 201 56 22 140 32 0 85 92 16 36 22 988 48 110 61 0 629 175 30 190 751 58 80 Height 1990 1179 3664 2690 1331 8484 10506 15431 108 4011 2989 384 757539 286801 320936 34951 35006 40523 26847 92954 269675 289170 274055 543049 44032 102015 IS Height Sample size 92954 2.0400 269675 2.0400 269675 2.0400 269675 2.0400 757539 2.0400 757539 2.0400 289170 2.0400 274055 2.0400 274055 2.0400 543049 2.0400 40523 2.0400 26847 2.0400 40523 2.0400 26847 2.0400 40523 2.0400 34951 2.0400 35006 2.0400 44032 2.0400 320936 2.0400 320936 2.0400 2.0400 320936 2.0400 320936 2.0400 320936 2.0400 320936 2.0400 320936 2.0400 320936 2.0400 320936 2.0400 320936 320936 2.0400 2.0400 320936 2.0400 2.0400 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 MMI 1.0 1.0 MM 1.0 1.0 bbl 1.0 MM 1.0 bb 1.0 MM 1.0 1.0 1.0 1.0 MMI 1.0 1.0 MM 1.0 bb 1.0 MMI 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 139 of 234 .axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G__013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: W G 42409-101,,BLK Name: FC3G JJ13S020 Description: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 17:52:20 PFBA FC3G 013S020 PFPeA FC3G 013S020 PFNA FC3G 013S020 P F H xS -1 FC3G 013S020 PFDA FC3G_013S020 100^- 8.05 r 1-11e3 3948 %- 12.26 11.25. i_2f'v'WwV' 8.00 10.00 12.00 P FH xS -2 FC3G_013S020 100- I 7.33 _ 53e1^ 141 A %- - 6.71 7.00 8.08 8.00 PFHxA FC3G_013S020 100- 6.36 9.37e2 4789 %- 4.80 5.06 , 5.00 6.00 7.00 PFUnA FC3G_013S020 100 _ 8.55 1.93e2 12.02 1064 A %- i io.77^( 'vIn -AWA wj........ 8.00 10.00 12.00 P F H xS -3 FC3G 013S020 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S020 100n 3.68 6.64e2 2397 % 5.54 6'40 ' 0^ - min 5.00 6.00 7.00 PFOA-1 FC3G 013S020 100- 7.09 J7.09e2 \ 2235 %~ \ 7.84__ 0- .1,.... , , , ....1......, 7.00 8.00 PFDoA FC3G 013S020 PFBS-1 PFOA-2 FC3G_013S020 7.00 PFBS-2 min 8.00 PFOS-1 FC3G 013S020 PFOS-2 FC3G 013S020 100- 1 1 .0 7 j1 .6 0 ,11.96 9.36 %- 8.29 |i J V PFOSA FC3G_013S020 8.00 10.00 12.00 e li Page 140 of 234 .axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnstJ3\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: W G 42409-101,,B LK Name: FC3G_013S020 Description: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 17:52:20 1 3 C 2 -P FO A FC3G_013S020 100-1 7 r 2.10e5 844623 13C 8 -P FO A FC3G 013S020 100- f 6.58e4 262742 %-_ 13C 4 -P FO A FC3G_013S020 100q : 7.09 I" 8.09e4 .314376 %~ 4 ___ -n min 7.00 8.00 0- t47.00 . - n min 8.00 o7.00 8.00 1 8 0 2 -P FH xS -2 FC3G 013S020 13C4-PFBA FC3G 013S020 100 5.26 T 3.21e4 1 3 C 2 -P F H x A FC3G_013S020 6.34 _ 7.07e4 339817 1 3C 8-P FO SA FC3G_013S020 100- 9.99 1.14e4l 55488 5.00 6.00 7.00 1 3 C 2 -P FO U E A FC3GJM3S020 100- 7.43 z54e4l '.'6663, %- 8.00 10.00 12.00 7.00 8.00 5.00 6.00 7.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 1 3 C 4-P FO S-1 FC3G 013S020 1 3 C 4 -P F O S-2 FC3G013S020 100 _ 8.35 8.50e3 31304 %- 1 8 0 2 -P FH X S -1 FC3G_013S020 100- 17.0383e4*l 44845 S %- ................. ....... r 8.00 10.00 12.00 7.00 8.00 1 3 C 5 -P FN A FC3G_013S020 7 AO 100 6.94e4_| 277702 1 r f T r t ' i min 7.00 8.00 1 3 C 2 -P F D A FC3G 013S020 100 6.66e4 1 234801 %- , ,,0- L min 8.00 10.00 12.00 1 3 C 2 -P F D o A FC3G_013S020 100q 9.18 T 1.07e5 1 493306 0-* 8.00 10.00 12.00 m Page 141 of 234 www.axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: W G 42409-101,,BLK Name: FC3G_013S020 Description: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 17:52:20 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.28 27 262.98 >218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 7.14 28 462.96 >418.... 29 512.96 >468.... 8.05 29 562.96 >518.... 8.55 30 612.95 >568.... 9.21 24 298.94> 79.96 25 298.94 > 98.95 6.38 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 22 498.93> 79.96 8.23 23 498.93> 98.96 31 498 > 78 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.26 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 8.05 21 614.95 >569.... 9.18 21 506>78 9.99 458.98 >393.98 7.43 Area 670 166 937 664 709 190 1112 193 411 10 46 65 329 210191 65827 80937 10934 8497 10836 7816 32061 70653 69441 66623 106551 11409 25359 IS Area 32061 70653 70653 70653 210191 210191 69441 66623 66623 106551 10836 7816 10836 7816 10836 10934 8497 11409 80937 80937 80937 80937 80937 80937 80937 80937 80937 80937 80937 80937 ng/g 0.020 0.005 0.096 0.033 0.099 40.710 6.512 5.223 21.136 20.147 21.346 23.150 19.347 17.837 13.052 9.368 9.078 10.384 4.485 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0259 0.0314 0.1137 0.0044 0.0231 0.1374 0.1672 0.0493 0.3331 0.2437 0.0219 0.0100 0.0499 0.0450 0.0268 0.0468 %Rec S/N 7 3 21 12 13 6 10 5 7 3 42 3 7 113.1 108.5 87.1 117.4 111.9 118.6 128.6 161.2 148.6 108.8 78.1 75.7 86.5 89.7 1112 4574 678 468 343 1384 211 234 3024 4042 511 722 1457 287 Noise 286 308 226 192 169 131 288 382 203 258 36 , 23 4 102 17 111 159 7 760 57 463 92 91 32 146 523 112 69 460 684 38 336 Height 2046 807 4789 2397 2235 775 3948 1064 1697 80 179 341 778 844623 262742 314376 43024 31304 44845 30769 122462 339817 277702 234801 493306 55488 96663 IS Height 122462 339817 339817 339817 844623 844623 277702 234801 234801 493306 44845 30769 44845 30769 44845 43024 31304 55488 314376 314376 314376 314376 314376 314376 314376 314376 314376 314376 314376 314376 Sample size 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 bbl 1.0 MM 1.0 bbl 1.0 MM 1.0 1.0 MMI 1.0 MMI 1.0 MMI 1.0 1.0 MMI 1.0 MM 1.0 MMI 1.0 1.0 MM 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 142 of 234 vww.axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst__G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: W G42409-102,,SPM Name: FC3G_013S017 Description: 1,W G42409,15/4000uL Date: 11-Jan-2013 Tim e: 17:01:25 PFBA FC3G_013S017 100- F 4.51 e4 1 174050 %M -r? min 5.00 6.00 7.00 PFPeA FC3G 013S017 100- %-: 6.15e4 282976 I-I 0 - I L n Tn-rr min 5.00 6.00 7.00 PFHxA FC3G_013S017 1 0 0 -q 6.36 0- 5.00 6.00 7.00 PFNA FC3G_013S017 ~I cc 100 1 1.37e5 ' 514423 %- PFDA FC3G 013S017 100-| 1.76e5 628490 PFUnA FC3G_013S017 100q 8.58 2.06e5 904322 %: min o: i L . - min 0: 7.00 8.00 8.00 10.00 12.00 8.00 10.00 12.00 P F H xS -1 FC3G_013S017 100n 2 99e4 112285 --------------- . , -r min 7.00 8.00 P FH xS -2 FC3G__013S017 100- 1.68e4_ i 62536 . %- m | /fllllllliilltkiS 0- t -t min 7.00 8.00 P F H xS -3 FC3GJ113S017 10 0^ 7.33 9.97e3~l 37443 / 1 %- o7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S017 100-, fi ' 1.19e5 462166 J %- I \ min 5.00 6.00 7.00 PFOA-1 FC3G 013S017 Mrt--~ 1.'29e5 ; J 499382 %"" m 0 7.00 min 8.00 PFOA-2 FC3G_013S017 1,0u0u-1- 7.14 F 2.97e4 I 113690 %- Ml 0 7.00 8.00 PFDoA FC3G 013S017 100- 9.21 2.27e5 902977 %: PFBS-1 FC3G 013S017 100- 6.47 3.25e4 144504 i %J l ' r min 8.00 10.00 12.00 I 11' T 11T 111" " r ' *! 5.00 6.00 7.00 PFBS-2 FC3G 013S017 100- 6.47 1.77e4 82457 J\ V min 5.00 6,00 7.00 PFOS-1 FC3G_013S017 PFOS-2 FC3G_013S017 100- 8.35 r 1.79e4 57492 %- 4Q -1 8.00 10.00 12.00 PFOSA FC3G_013S017 100- 9.99 2.44e4 88663 j %I JL 8.00 10.00 ][ Page 143 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3GJD13-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: WG42409-102,,SPM Name: FC3G_013S017 Description: 1,WG42409,15/4000uL Date: 11>Jan-2013 Time: 17:01:25 13C2-PFOA FC3G_013S017 100- 7.09 f 2.46e5 980226 %- 13C8-PFOA FC3G 013S017 100- 7.09 8.29e4 332713 %- 13C4-PFOA FC3GJ313S017 100- 7.09 i 8.77e4 11334357 %- 7.00 8.00 7.00 8.00 7.00 8.00 1802-PFHxS-2 FC3G_101Q3QS017 100 t7.87e3 ' : 32631 .. 7.00 -TT min 8.00 13C4-PFBA FC3G_013S017 100- 1"2.64e4 99528 %: 4.03 0^ ,uTT^rfrrrrrrr min 5.00 6.00 7.00 13C2-PFHxA FC3G_013S017 100-1 6.36 6.51e4 305374 - 0: 5.00 6.00 7.00 13C8-PFOSA FC3G_013S017 100- 9.99 1.55e4~] 79247 l %- i 13C2-PFOUEA FC3G 013S017 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S017 100-] 8.35 p-24e4 I 49463 %- I JLi m 111''rT rT mln 8.00 10.00 12.00 13C4-PFOS-2 FC3GJ113S017 100-1 8.35 F 9.24e3 1 36537 %7 i I, 8.00 104)0 12.00 1802-PFHxS-1 FC3G_013S017 100- 7.33 1,09e4 43281 M"\ %- ,1' ' inn 111 min 7.00 8.00 13C5-PFNA FC3G_013S017 100-1 7.55 9.19e4 1 3607611 A%f f r r i min 7.00 8.00 13C2-PFDA FC3G_013S017 -,100 8.05 1.01e5 I! 372440 ii min 8.00 10.00 12.00 13C2-PFDOA FC3G013S017 10 Page 144 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-102,,SPM Name: FC3GJJ13S017 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 17:01:25 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.23 27 262.98 >218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 >418.... 7.55 29 512.96 >468.... 8.05 29 562.96 >518.... 8.58 30 612.95 >568.... 9.21 24 298,94> 79.96 6.47 25 298.94 > 98.95 6.47 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.19 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 >569.... 9.21 21 506>78 9.99 458.98 >393.98 7.43 Area 45106 61531 120536 118685 128693 29652 137069 176239 206279 226758 32543 17750 29932 16817 9968 29013 17866 24373 246349 82938 87669 12387 9238 10919 7871 26386 65120 91883 100764 151683 15475 28591 IS Area 26386 65120 65120 65120 246349 246349 91883 100764 100764 151683 10919 7871 10919 7871 10919 12387 9238 15475 87669 87669 87669 87669 87669 87669 87669 87669 87669 87669 87669 87669 ng/g 34.796 31.412 35.989 31.644 34.765 35.441 31.093 34.017 33.039 39.920 78.469 75.827 74.487 73.172 75.695 65.612 64.577 34.765 88.100 15.148 11.315 44.213 40.445 39.717 43.047 29.398 30.356 31.889 26.1.62 23.863 26.008 10.112 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0641 0.0121 0.0322 0.0862 0.0051 0.0459 0.2677 0.1600 0.0176 0.6565 0.0323 0.0534 0.0912 0.1337 0.0463 0.0539 %Rec 87.0 78.5 90.0 79.1 86.9 88.6 77.7 85.0 82.6 99.8 98.1 94.8 93.1 91.5 94.6 82.0 80.7 86.9 122.4 126.2 94.3 122.8 112.3 110.3 119.6 122.5 126.5 132.9 109.0 99.4 108.4 101.1 S/N Noise 771 226 899 315 3025 191 1685 274 2420 206 1389 82 665 773 1645 382 3835 236 2636 343 2180 66 4477 18 2569 44 370 169 00 531 161 481 119 1756 . 51 3199 306 9455 . 35 739 452 519 95 786 46 7043 6 00 133 749 3464 88 1843 196 834 447 593 2260 1080 35 563 192 Height 174050 282976 576976 462166 499382 113690 514423 628490 904322 902977 144504 82457 112285 62536 37443 85506 57492 88663 980226 332713 334357 49463 36537 43281 32631 99528 305374 360761 372440 640853 79247 108208 IS Height 99528 305374 305374 305374 980226 980226 360761 372440 372440 640853 43281 32631 43281 32631 43281 49463 36537 79247 334357 334357 334357 334357 334357 334357 334357 334357 334357 334357 334357 334357 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection.., 1.0 bb 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1,0 bb 1.0 bb 1.0 bb Page 145 of 234 www.axysanalytical.com ___________ fll Sample List Report T :- Ssuu- ________ ._______________________ MassLynx 4.1 SCN627 Sample List; G:\Masslynx\lnst_G\_Master.PRO\SampleDB\FC3G_013.SPL Last Modified: Friday, January 11,2013 13:01:07 Pacific Standard Time Printed: Friday, January 11, 2013 13:05:15 Pacific Standard Time Page 1of 1 Page Position (1,1) File Name Sample ID File Text Bottle Signfn SignOut Inj VoL 1 FC3G 013S001 MeOH,, 1,,15uL 2 FC3G 013S002 MeOH,, 1,,15uL 3 FC3G 013S003 FC-LIN-42151T-A,,1/2 1,,15uL 4 FC3G 013S004 FC-LIN-42151T-A,,1/2 1,,15uL 5 FC3G 013S005 FC-LIN-42151T-B,,1/2 1,,15uL 6 FC3G 013S006 FC-LIN-42151T-C,,1/2 1,,15uL 7 FC3G 013S007 FC-LlN-42151T-D,,1/2 1,,15uL 8 FC3G 013S008 FC-LIN-42151T-E,,1/2 1,,15uL 9 FC3G 013S009 FC-LIN-42151T-F,,1/2 1,,15uL 10 FC3G 013S010 FC-LIN-42151T-G,,1/2 1,,15uL 11 FC3G 013S011 FC-LIN-42151T-H,,1/2 1,,15uL 12 FC3G 013S012 MeOH,, 1,,15uL 1:8,A L-IU1:8,A - 2:1,A 2:1,A - 2:1,B - 2:1,C - 2:1,D - 2:1,E - 2:1,F ~J2:2,A - - 2:2,B 1:8,A - i"i/-4 --- -,----- --M --- 15.0 15.0 15.0 15.0 -, 15.0 15.0 15.0 V 15.0 15.0. 15.0 15.0 J 15.0 13 FC3G 013S013 MeOH,, 1,,15uL 14 FC3G 013S014 FC-LIN-42151T-DJ/2 1,,15uL 15 FC3G 013S015 FC-LIN-42151T-D,,1/2 1,,15uL 1:8,A 2:1,D - 1 T 15.0 15.0 2:1,D - 15.0 16 FC3G 013S016 WG42409-104,,SAR 1,WG42409,15/4000uL 3:4,A - 15.0 17 FC3G 013S017 WG42409-102,,SPM 1,WG42409,15/4000uL 3:4,B - 18 FC3G 013S018 MeOH,, 1,,15uL 1:8,A - "1 15.0 15.0 19 FC3G 013S019 MeOH,, 1,,15uL ' 1:8,A - - x 15.0 \ 20 FC3G 013S020 WG42409-101,,BLK 1,WG42409,15/4000uL 3:4,C - 15.0 21 FC3G 013S021 L19064-1,, 1,WG42409,15/4000uL 3:4,D - - 15.0 22 FC3G 013S022 L19064-2,,. 1,WG42409,15/4000uL 3:4,E - -- 15.0 23 FC3G 013S023 L19064-3,, 1,WG42409,15/4000uL 3:4,F - -- 15.0 24 FC3G 013S024 L19064-4,, 25 FC3G 013S025 L19064-5,, 1,WG42409,15/4000uL 3:5,A 1,WG42409,15/4000uL 3:5,B - - -- 15.0 15.0 26 FC3G 013S026 L19064-6,, 27 FC3G 013S027 L19064-7,,. 28 FC3G 013S028 L19064-8,, 29 FC3G 013S029 L19064-9,, 30 FC3G 013S030 L19064-10,, 31 FC3G 013S031 L19064-11,, 32 FC3G 013S032 L19064-12,, 33 FC3G 013S033 L19064-13,, 34 FC3G 013S034 L19064-14,, 35 FC3G 013S035 WG42409-103..DUP 36 FC3G 013S036 L19064-15,, 37 FC3G 013S037 L19064-16,, 38 FC3G 013S038 FC-LIN-42151T-D,,1/2 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000UL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,,15uL 3:5,C 3:5,D 3:5,E 3:5,F 3:6,A 3:6,B 3:6,C 3:6,D 3:6,E 3:6,F 3:7,A 3:7,B 2:1,D --- I*' -_ ----_ ------ - ~ -- . -,,,, -- -- ~ -- MM MM 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15,0 15.0 15,0 15.0 39 FC3G 013S039 FC-LIN-42151T-D,,1/2 1,,15uL 40 FC3G 013S040 MeOH,, 1,,15uL 2:1,D -t 1:8,A Y / -J 15.0 15.0 Al t c ' I iVG G & ( ^LS> [ 2 3 -a K 2 o U Page 146 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Method: G:\Masslynx\Master Methods\PFC\FC_043_08_02.mdb 06 Nov 2012 13:32:38 Calibration: 14 Jan 2013 13:08:53 Compound name: PFBA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 5.24 5.24 5.26 5.26 5.24 5.24 5.21 5.26 Area 2349 4251 10366 45779 255608 529256 1106351 1451154 IS Area 23694 25135 22826 23689 24224 26173 21823 13033 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 1.017 2.485 8.463 39.474 220.277 423.109 1062.375 2334.523 %Rec 101.7 99.4 84.6 98.7 110.1 105.8 106.2 93.4 Compound name: PFBA Correlation coefficient: r = 0.996181, rA2 = 0.992377 Calibration curve: 1.14415 * x + 1.21576 Response type: Internal Std ( Ref 26 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 38 32 189 460 124 1863 18584 4954 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 147 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFPeA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 5.99 6.01 5.99 5.99 5.97 5.97 5.95 5.97 Area 2008 5123 14550 67138 336833 733203 1451118 2412291 IS Area 64291 64250 58083 62914 55802 52550 43564 33387 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 1.001 2.615 8.299 35.482 200.881 464.376 1108.718 2404.932 %Rec 100.1 104.6 83.0 88.7 100.4 116.1 110.9 96.2 Compound name: PFPeA Correlation coefficient: r = 0.993143, rA2 = 0.986334 Calibration curve: 0.721029 * x + 0.0279352 Response type: Internal Std ( Ref 27 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 37 195 596 1692 7323 10162 63240 30997 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 148 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHxA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.38 6.38 6.38 6.36 6.34 6.36 6.34 6.34 Area 4568 9975 27425 130876 610449 1180792 2301871 3564080 IS Area 64291 64250 58083 62914 55802 52550 43564 33387 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.987 2.643 8.875 40.497 214.713 441.448 1038.652 2098.793 %Rec 98.7 105.7 88.8 101.2 107.4 110.4 103.9 84.0 Compound name: PFHxA Correlation coefficient: r = 0.995059, rA2 = 0.990142 Calibration curve: 1.22047 * x + 0.500033 Response type: Internal Std ( Ref 27 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 143 167 598 1704 6667 20565 39460 45309 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 149 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHpA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.73 6.78 6.73 6.73 6.73 6.78 6.73 6.73 Area 4308 9766 28576 146028 638000 1404129 2756213 4155998 IS Area 64291 64250 58083 62914 55802 52550 43564 33387 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 1.015 2.498 8.430 40.341 199.318 466.012 1103.663 2171.579 %Rec 101.5 99.9 84.3 100.9 99.7 116.5 110.4 86.9 Compound name: PFHpA Correlation coefficient: r = 0.993371, rA2 = 0.986786 Calibration curve: 1.37563 * x + 0.211383 Response type: Internal Std ( Ref 27 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 73 182 692 2276 10287 25207 73556 34263 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 150 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOA-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.14 7.14 7.14 7.14 7.14 7.14 7.09 Area 4305 10427 29956 146755 720547 1356679 2785893 4425141 IS Area 237751 239566 212071 256975 219864 218928 178447 132546 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.979 2.682 9.230 38.027 219.330 414.938 1045.711 2236.502 Compound name: PFOA-1 Correlation coefficient: r = 0.996840, rA2 = 0.993690 Calibration curve: 1.07468 * x + 0.251326 Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None %Rec 97.9 107.3 92.3 95.1 109.7 103.7 104.6 89.5 S/N 123 362 487 6798 41534 25056 11748 9648 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 151 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOA-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.19 7.14 7.14 7.14 7.14 7.14 7.09 Area 869 2142 6254 33767 163932 315010 654423 1008404 IS Area 237751 239566 212071 256975 219864 218928 178447 132546 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 1.004 2.563 8.626 38.698 219.931 424.491 1082.038 2244.799 %Rec 100.4 102.5 86.3 96.7 110.0 106.1 108.2 89.8 S/N 0 566 385 0 0 0 0 0 Compound name: PFOA-2 Correlation coefficient: r = 0.995736, rA2 = 0.991490 Calibration curve: 0.244011 * x + 0.018253 Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 152 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFNA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.61 7.61 7.55 7.55 7.55 7.55 7.55 7.49 Area 4448 11290 32114 148161 791016 1497967 3206580 4992039 IS Area 81603 84569 67753 83045 74331 81811 64985 44026 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.982 2.637 9.770 37.217 222.788 383.440 1033.584 2375.365 %Rec 98.2 105.5 97.7 93.0 111.4 95.9 103.4 95.0 Compound name: PFNA Correlation coefficient: r = 0.997754, rA2 = 0.995513 Calibration curve: 1.14558 * x + 0.183555 Response type: Internal Std ( Ref 28 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 30 107 212 1221 3532 5263 13098 29400 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 153 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFDA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.11 8.11 8.05 8.05 8.05 8.05 8.05 8.05 Area 7175 15035 42575 190115 1042945 2117893 4358040 6485407 IS Area 101067 86238 84230 93912 96533 102880 98693 85123 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.930 2.968 9.497 39.447 212.560 405.438 870.216 1501.794 %Rec 93.0 118.7 95.0 98.6 106.3 101.4 87.0 60.1 Compound name: PFDA Correlation coefficient: r = 0.993688, rA2 = 0.987415 Calibration curve: 1.21718 * x + 0.571428 Response type: Internal Std ( Ref 29 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 153 159 465 1408 6927 8285 1730 10240 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MMX PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 154 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFUnA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.67 8.70 8.64 8.61 8.61 8.64 8.61 8.61 Area 7307 16832 47553 234886 1347697 2783679 6297988 9320023 IS Area 101067 86238 84230 93912 96533 102880 98693 85123 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.940 2.937 8.941 40.418 226.688 439.560 1037.007 1779.414 %Rec 94.0 117.5 89.4 101.0 113.3 109.9 103.7 71.2 Compound name: PFUnA Correlation coefficient: r = 0.987103, rA2 = 0.974372 Calibration curve: 1.47655 * x + 0.347898 Response type: Internal Std ( Ref 29 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 142 245 1332 4601 6793 21784 15999 19203 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 155 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFDoA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 9.27 9.30 9.27 9.24 9.24 9.27 9.24 9.24 Area 10194 22074 57475 286387 1640496 3481032 8082436 14006239 IS Area 169868 163299 142436 174079 191720 214405 246180 246757 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.920 2.963 10.256 44.003 231.852 440.561 891.613 1542.003 %Rec 92.0 118.5 102.6 110.0 115.9 110.1 89.2 61.7 Compound name: PFDoA Correlation coefficient: r = 0.980111, rA2 = 0.960617 Calibration curve: 0.883036 * x + 0.627837 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 226 490 876 3927 11958 4243 37235 81743 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 156 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFBS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.53 6.53 6.51 6.51 6.49 6.51 6.49 6.47 Area 1038 2683 7781 37888 178976 333040 616948 910658 IS Area 12951 13585 11291 13066 11125 9932 7971 5135 Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 2.005 5.098 18.060 76.343 424.077 884.007 2040.651 4675.857 %Rec 100.2 102.0 90.3 95.4 106.0 110.5 102.0 93.5 Compound name: PFBS-1 Correlation coefficient: r = 0.997410, rA2 = 0.994826 Calibration curve: 1.3654 * x + 0.148867 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 127 187 768 9176 16927 47040 35111 31464 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 157 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFBS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.49 6.53 6.49 6.49 6.49 6.51 6.49 6.47 Area 682 1639 4065 20644 93640 176912 325842 464895 IS Area 8069 8402 7204 8344 6980 7888 5514 3683 Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.907 5.672 18.249 83.291 455.894 762.814 2011.315 4298.276 %Rec 95.3 113.4 91.2 104.1 114.0 95.4 100.6 86.0 Compound name: PFBS-2 Correlation coefficient: r = 0.994125, rA2 = 0.988285 Calibration curve: 1.0571 * x + 1.02602 Response type: Internal Std ( Ref 25 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 89 193 514 3296 4810 15977 36917 27854 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 158 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHxS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.33 7.38 7.38 7.38 7.33 7.33 Area 995 2472 7449 35921 174989 321443 630934 875468 IS Area 12951 13585 11291 13066 11125 9932 7971 5135 Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 2.040 4.897 17.890 74.702 427.668 879.991 2152.301 4635.939 %Rec 102.0 97.9 89.4 93.4 106.9 110.0 107.6 92.7 Compound name: PFHxS-1 Correlation coefficient: r = 0.996495, rA2 = 0.993002 Calibration curve: 1.32396 * x + 0.0663729 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 48 232 179 766 6713 3256 19148 4897 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 159 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHxS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.38 7.33 7.38 7.38 7.33 7.33 Area 583 1506 3685 19357 86452 183159 345834 486835 IS Area 8069 8402 7204 8344 6980 7888 5514 3683 Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.929 5.624 17.091 79.505 426.879 800.785 2163.792 4561.884 %Rec 96.4 112.5 85.5 99.4 106.7 100.1 108.2 91.2 S/N 144 0 0 0 0 0 0 0 Compound name: PFHxS-2 Correlation coefficient: r = 0.995281, rA2 = 0.990585 Calibration curve: 1.04313 * x + 0.587258 Response type: Internal Std ( Ref 25 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 160 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHxS-3 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.33 7.33 7.33 7.38 7.33 7.33 Area 401 953 2264 12138 56878 102356 208209 289888 IS Area 12951 13585 11291 13066 11125 9932 7971 5135 Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.996 5.269 16.164 77.034 426.669 860.628 2182.301 4717.185 %Rec 99.8 105.4 80.8 96.3 106.7 107.6 109.1 94.3 Compound name: PFHxS-3 Correlation coefficient: r = 0.994802, rA2 = 0.989632 Calibration curve: 0.430793 * x + 0.255689 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 0 63 0 0 0 0 77975 0 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 161 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.44 8.44 8.41 8.41 8.35 8.41 8.35 8.35 Area 1136 2550 6349 31778 164663 339460 744593 1072351 IS Area 12280 11889 10194 11195 11328 12244 9275 6391 Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.964 5.415 16.969 79.662 410.612 783.790 2270.578 4746.605 %Rec 98.2 108.3 84.8 99.6 102.7 98.0 113.5 94.9 S/N 34 62 108 355 1797 4253 4771 2634 Compound name: PFOS-1 Correlation coefficient: r = 0.995671, rA2 = 0.991361 Calibration curve: 1.2724 * x + 0.830661 Response type: Internal Std ( Ref 22 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 162 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.44 8.44 8.41 8.41 8.41 8.41 8.35 8.35 Area 773 1784 4931 21613 108199 241234 502724 748686 IS Area 9118 9407 8158 10080 9161 9863 8107 5168 Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.932 5.483 19.539 71.699 399.187 827.661 2099.973 4907.534 %Rec 96.6 109.7 97.7 89.6 99.8 103.5 105.0 98.2 S/N 46 59 176 745 1585 2892 5738 3303 Compound name: PFOS-2 Correlation coefficient: r = 0.997851, rA2 = 0.995706 Calibration curve: 1.06261 * x + 0.999871 Response type: Internal Std ( Ref 23 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 163 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOSA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 9.96 9.99 9.99 9.96 9.99 9.99 9.99 9.99 Area 1074 1891 5955 28377 177335 364640 935573 1753377 IS Area 15418 14099 14395 16141 18940 19590 19776 15345 Std. Conc 1.200 2.700 10.200 40.000 200.000 400.000 1000.000 2500.000 ng/g 1.226 2.663 8.891 38.844 208.283 414.386 1053.749 2545.530 %Rec 102.2 98.6 87.2 97.1 104.1 103.6 105.4 101.8 Compound name: PFOSA Correlation coefficient: r = 0.997950, rA2 = 0.995904 Calibration curve: 1.07718 * x + 0.35077 Response type: Internal Std ( Ref 31 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None S/N 138 280 535 1329 5818 3875 9816 24758 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 164 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFOA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.14 7.14 7.14 7.14 7.14 7.09 7.09 Area 237751 239566 212071 256975 219864 218928 178447 132546 Compound name: 13C2-PFOA Response Factor: 0.382748 RRF SD: 0.0155651, % Relative SD: 4.06668 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 72.000 72.000 72.000 72.000 72.000 72.000 72.000 72.000 ng/g 67.507 70.606 72.985 68.946 71.975 74.028 73.284 76.671 %Rec 93.8 98.1 101.4 95.8 100.0 102.8 101.8 106.5 S/N 11115 2708 3477 1889 2754 3151 2103 2134 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 165 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C8-PFOA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.14 7.14 7.14 7.14 7.14 7.09 7.09 Area 86222 75200 68945 81521 72926 71423 57989 41290 Compound name: 13C8-PFOA Response Factor: 0.749417 RRF SD: 0.030168, % Relative SD: 4.02553 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/g 12.503 11.319 12.118 11.171 12.193 12.334 12.163 12.198 %Rec 104.2 94.3 101.0 93.1 101.6 102.8 101.4 101.7 S/N 1143 4719 2795 3552 4603 4024 3619 1227 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 166 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C4-PFOA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.14 7.14 7.14 7.09 7.14 7.09 7.09 Compound name: 13C4-PFOA Response Factor: 7747.83 RRF SD: 1680.24, % Relative SD: 21.6866 Response type: External Std, Area Curve type: Rf Area 110419 106379 91100 116856 95773 92721 76343 54200 IS Area Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/g 14.252 13.730 11.758 15.082 12.361 11.967 9.853 6.996 %Rec 118.8 114.4 98.0 125.7 103.0 99.7 82.1 58.3 S/N 575 2948 115 308 392 1290 307 718 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 167 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C4-PFOS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.41 8.44 8.41 8.41 8.41 8.41 8.35 8.41 Area 12280 11889 10194 11195 11328 12244 9275 6391 Compound name: 13C4-PFOS-1 Response Factor: 0.0383506 RRF SD: 0.00346218, % Relative SD: 9.0277 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/g 34.798 34.971 35.015 29.976 37.010 41.318 38.017 36.896 %Rec 96.7 97.1 97.3 83.3 102.8 114.8 105.6 102.5 S/N 937 274 532 460 739 654 510 446 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 168 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C4-PFOS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.41 8.44 8.41 8.41 8.41 8.41 8.35 8.35 Area 9118 9407 8158 10080 9161 9863 8107 5168 Compound name: 13C4-PFOS-2 Response Factor: 0.0312656 RRF SD: 0.00294722, % Relative SD: 9.42639 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/g 31.694 33.939 34.368 33.108 36.713 40.828 40.756 36.593 %Rec 88.0 94.3 95.5 92.0 102.0 113.4 113.2 101.6 S/N 308 321 376 781 334 628 378 418 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 169 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 18O2-PFHxS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.38 7.38 7.33 7.38 7.33 7.33 Area 12951 13585 11291 13066 11125 9932 7971 5135 Compound name: 18O2-PFHxS-1 Response Factor: 0.0376316 RRF SD: 0.00358002, % Relative SD: 9.51333 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/g 37.400 40.722 39.522 35.656 37.040 34.157 33.293 30.210 %Rec 103.9 113.1 109.8 99.0 102.9 94.9 92.5 83.9 S/N 990 1330 770 824 5201 1211 689 111 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 170 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 18O2-PFHxS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.38 7.38 7.33 7.38 7.33 7.33 Area 8069 8402 7204 8344 6980 7888 5514 3683 Compound name: 18O2-PFHxS-2 Response Factor: 0.0250277 RRF SD: 0.00183648, % Relative SD: 7.33779 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/g 35.038 37.870 37.913 34.234 34.945 40.790 34.633 32.577 %Rec 97.3 105.2 105.3 95.1 97.1 113.3 96.2 90.5 S/N 255 0 2225 464 823 2137 1550 525 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 171 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C4-PFBA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 5.24 5.24 5.28 5.24 5.26 5.26 5.23 5.24 Area 23694 25135 22826 23689 24224 26173 21823 13033 Compound name: 13C4-PFBA Response Factor: 0.122853 RRF SD: 0.014587, % Relative SD: 11.8735 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 20.960 23.079 24.474 19.801 24.706 27.572 27.921 23.488 %Rec 87.3 96.2 102.0 82.5 102.9 114.9 116.3 97.9 S/N 110 88 130 98 115 230 129 56 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM bb MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 172 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFHxA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.36 6.38 6.36 6.36 6.34 6.36 6.34 6.33 Area 64291 64250 58083 62914 55802 52550 43564 33387 Compound name: 13C2-PFHxA Response Factor: 0.293638 RRF SD: 0.0155463, % Relative SD: 5.29437 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 23.794 24.682 26.055 22.002 23.811 23.162 23.320 25.174 %Rec 99.1 102.8 108.6 91.7 99.2 96.5 97.2 104.9 S/N 1775 2646 1262 1397 1189 1905 770 1475 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 173 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C5-PFNA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.55 7.61 7.55 7.55 7.55 7.55 7.55 7.49 Area 81603 84569 67753 83045 74331 81811 64985 44026 Compound name: 13C5-PFNA Response Factor: 0.394397 RRF SD: 0.029284, % Relative SD: 7.425 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 22.486 24.188 22.628 21.623 23.614 26.846 25.900 24.714 %Rec 93.7 100.8 94.3 90.1 98.4 111.9 107.9 103.0 S/N 1290 986 1626 2215 2591 2045 1284 1468 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 174 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFDA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.11 8.11 8.05 8.05 8.05 8.05 8.05 8.05 Area 101067 86238 84230 93912 96533 102880 98693 85123 Compound name: 13C2-PFDA Response Factor: 0.527187 RRF SD: 0.131866, % Relative SD: 25.0131 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 20.834 18.453 21.046 18.293 22.943 25.256 29.426 35.749 %Rec 86.8 76.9 87.7 76.2 95.6 105.2 122.6 149.0 S/N 862 1222 817 844 946 932 1190 597 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 175 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFDoA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 9.27 9.30 9.24 9.24 9.24 9.24 9.24 9.21 Area 169868 163299 142436 174079 191720 214405 246180 246757 Compound name: 13C2-PFDoA Response Factor: 0.870071 RRF SD: 0.169205, % Relative SD: 19.4472 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 21.217 21.172 21.564 20.546 27.609 31.892 44.474 62.790 %Rec 88.4 88.2 89.8 85.6 115.0 132.9 185.3 261.6 S/N 737 746 874 625 1044 1103 1399 2038 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MMX MMX PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 176 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C8-PFOSA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 9.96 9.99 9.96 9.96 9.96 9.99 9.99 9.99 Area 15418 14099 14395 16141 18940 19590 19776 15345 Compound name: 13C8-PFOSA Response Factor: 0.0814461 RRF SD: 0.0168185, % Relative SD: 20.6499 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 20.573 19.527 23.281 20.351 29.138 31.130 38.165 41.713 %Rec 85.7 81.4 97.0 84.8 121.4 129.7 159.0 173.8 S/N 1133 2203 1154 1646 1366 1835 2244 1157 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MMX MMX PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 177 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFOUEA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.43 7.46 7.43 7.43 7.43 7.43 7.43 7.38 Compound name: 13C2-PFOUEA Response Factor: 2827.34 RRF SD: 398.089, % Relative SD: 14.08 Response type: External Std, Area Curve type: Rf Area 32596 29981 26306 30462 30315 30111 26510 19906 IS Area Std. Conc 10.000 10.000 10.000 10.000 10.000 10.000 10.000 10.000 ng/g 11.529 10.604 9.304 10.774 10.722 10.650 9.376 7.040 %Rec 115.3 106.0 93.0 107.7 107.2 106.5 93.8 70.4 S/N 2838 449 688 3001 656 2674 383 405 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 Acq.Time 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 178 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Method: G:\Masslynx\Master Methods\PFC\FC_043_08_02.mdb 06 Nov 2012 13:32:38 Calibration: 14 Jan 2013 13:08:53 ID: FC-LIN-42151T-A,,1/2 Name: FC3G_013S004 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:20:41 PFBA FC3G_013S004 5.24 100 2.35e3 8535 %- min 5.00 6.00 7.00 PFPeA FC3G_013S004 5.99 100 2.01e3 8800 % min 5.00 6.00 7.00 PFHxA FC3G_013S004 6.38 100 4.57e3 23646 % 4.16 min 5.00 6.00 7.00 PFHpA FC3G 013S004 PFNA FC3G 013S004 PFDA FC3G 013S004 PFUnA FC3G 013S004 PFDoA FC3G 013S004 PFOA-1 FC3G 013S004 min PFBS-1 FC3G 013S004 PFOA-2 FC3G 013S004 min PFBS-2 FC3G 013S004 min PFHxS-1 FC3G 013S004 min PFHxS-2 FC3G 013S004 min min min min PFHxS-3 FC3G 013S004 7.38 1004.01e2 1359 PFOS-1 FC3G_013S004 8.44 100 1.14e3 3697 PFOS-2 FC3G_013S004 8.44 100 7.73e2 2525 PFOSA FC3G 013S004 %6.63 / %% 12.23 min 0 7.00 8.00 m in 0 8.00 10.00 min 12.00 8.00 10.00 min 12.00 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 179 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-A,,1/2 Name: FC3G_013S004 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:20:41 13C2-PFOA FC3G 013S004 100- 7.14 2.38e5 963843 %- 13C8-PFOA FC3G 013S004 100- 7.14 8.62e4 339422 %- 13C4-PFOA FC3G_013S004 7.14 100 .10e5 433554 % 13C4-PFOS-1 FC3G 013S004 07.00 T-r min 8.00 18O2-PFHxS-2 FC3G 013S004 07.00 13C4-PFBA FC3G 013S004 t -t min 8.00 0 7.00 8.00 min 13C2-PFHxA FC3G 013S004 13C5-PFNA FC3G 013S004 13C4-PFOS-2 FC3G_013S004 8.41 100 9.12e3 38116 18O2-PFHxS-1 FC3G 013S004 min min 8.00 10.00 12.00 13C2-PFDA FC3G 013S004 13C2-PFDoA FC3G 013S004 min min min min min min min 13C8-PFOSA FC3G 013S004 13C2-PFOUEA FC3G 013S004 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 180 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-A,,1/2 Name: FC3G_013S004 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:20:41 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.24 27 262.98 > 218.... 5.99 27 312.97> 268.98 6.38 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.61 29 512.96 > 468.... 8.11 29 562.96 > 518.... 8.67 30 612.95 > 568.... 9.27 24 298.94> 79.96 6.53 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.38 24 398.84> 118.70 7.38 22 498.93> 79.96 8.44 23 498.93> 98.96 8.44 31 498 > 78 9.96 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.24 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.11 21 614.95 > 569.... 9.27 21 506>78 9.96 458.98 >393.98 7.43 Area 2349 2008 4568 4308 4305 869 4448 7175 7307 10194 1038 682 995 583 401 1136 773 1074 237751 86222 110419 12280 9118 12951 8069 23694 64291 81603 101067 169868 15418 32596 IS Area 23694 64291 64291 64291 237751 237751 81603 101067 101067 169868 12951 8069 12951 8069 12951 12280 9118 15418 110419 110419 110419 110419 110419 110419 110419 110419 110419 110419 110419 110419 ng/g 1.017 1.001 0.987 1.015 0.979 1.004 0.982 0.930 0.940 0.920 2.005 1.907 2.040 1.929 1.996 1.964 1.932 1.226 67.507 12.503 14.252 34.798 31.694 37.400 35.038 20.960 23.794 22.486 20.834 21.217 20.573 11.529 LOD 0.0359 0.0550 0.0188 0.0329 0.0743 0.1198 0.3287 0.1178 0.4347 0.5994 0.0502 0.0500 0.0683 0.0960 0.0706 0.0122 %Rec 101.7 100.1 98.7 101.5 97.9 100.4 98.2 93.0 94.0 92.0 100.2 95.3 102.0 96.4 99.8 98.2 96.6 102.2 93.8 104.2 118.8 96.7 88.0 103.9 97.3 87.3 99.1 93.7 86.8 88.4 85.7 115.3 S/N 38 37 143 73 123 0 30 153 142 226 127 89 48 144 0 34 46 138 11115 1143 575 937 308 990 255 110 1775 1290 862 737 1133 2838 Noise 223 235 165 247 141 0 555 176 233 197 39 40 68 17 0 110 55 30 87 297 753 55 124 53 131 887 177 238 434 1006 69 45 Height 8535 8800 23646 18016 17345 2964 16904 26972 32984 44631 5003 3568 3250 2504 1359 3697 2525 4108 963843 339422 433554 51864 38116 52855 33361 97299 314894 306487 373861 742142 78447 129081 IS Height 97299 314894 314894 314894 963843 963843 306487 373861 373861 742142 52855 33361 52855 33361 52855 51864 38116 78447 433554 433554 433554 433554 433554 433554 433554 433554 433554 433554 433554 433554 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 181 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-B,,1/2 Name: FC3G_013S005 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:37:42 PFBA FC3G_013S005 5.24 100 4.25e3 15643 %- PFPeA FC3G 013S005 min 5.00 6.00 7.00 PFNA FC3G 013S005 PFDA FC3G 013S005 PFHxA FC3G 013S005 min PFUnA FC3G 013S005 PFHpA FC3G 013S005 min PFDoA FC3G 013S005 PFOA-1 FC3G 013S005 min PFBS-1 FC3G 013S005 PFOA-2 FC3G 013S005 min PFBS-2 FC3G 013S005 min PFHxS-1 FC3G 013S005 min PFHxS-2 FC3G 013S005 min min PFHxS-3 FC3G 013S005 min min min min PFOS-1 FC3G_013S005 8.44 100 2.55e3 7396 % PFOS-2 FC3G_013S005 8.44 100 1 .78e3 6320 % PFOSA FC3G 013S005 12.02 min min min 8.00 10.00 12.00 8.00 10.00 12.00 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 182 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-B,,1/2 Name: FC3G_013S005 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:37:42 13C2-PFOA FC3G 013S005 100- 7.14 2.40e5 957565 %- ^40 - 7.00 T-r min 8.00 13C8-PFOA FC3G 013S005 100- 7.14 7.52e4 301233 %- 07.00 =rr min 8.00 13C4-PFOA FC3G_013S005 7.14 100 1 .06e5 1420 901 % 0 7.00 8.00 min 13C4-PFOS-1 FC3G 013S005 18O2-PFHxS-2 FC3G 013S005 13C4-PFBA FC3G_013S005 5.24 100 2.51e4 103637 % 13C2-PFHxA FC3G 013S005 13C5-PFNA FC3G 013S005 13C8-PFOSA FC3G_013S005 9.99 _ 1 00 - 1.41e4 74369 %- min min 5.00 6.00 7.00 13C2-PFOUEA FC3G 013S005 min 0 8.00 10.00 min 12.00 min 13C4-PFOS-2 FC3G 013S005 min 13C2-PFDA FC3G 013S005 min 18O2-PFHxS-1 FC3G 013S005 min 13C2-PFDoA FC3G 013S005 min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 183 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-B,,1/2 Name: FC3G_013S005 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:37:42 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.24 27 262.98 > 218.... 6.01 27 312.97> 268.98 6.38 27 362.97> 318.98 6.78 19 412.97> 368.98 7.14 19 412.97> 168.80 7.19 28 462.96 > 418.... 7.61 29 512.96 > 468.... 8.11 29 562.96 > 518.... 8.70 30 612.95 > 568.... 9.30 24 298.94> 79.96 6.53 25 298.94 > 98.95 6.53 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.38 24 398.84> 118.70 7.38 22 498.93> 79.96 8.44 23 498.93> 98.96 8.44 31 498 > 78 9.99 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.44 21 503 > 98.96 8.44 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.24 21 314.97>269.98 6.38 21 467.96>422.97 7.61 21 514.96 >469.96 8.11 21 614.95 > 569.... 9.30 21 506>78 9.99 458.98 >393.98 7.46 Area 4251 5123 9975 9766 10427 2142 11290 15035 16832 22074 2683 1639 2472 1506 953 2550 1784 1891 239566 75200 106379 11889 9407 13585 8402 25135 64250 84569 86238 163299 14099 29981 IS Area 25135 64250 64250 64250 239566 239566 84569 86238 86238 163299 13585 8402 13585 8402 13585 11889 9407 14099 106379 106379 106379 106379 106379 106379 106379 106379 106379 106379 106379 106379 ng/g 2.485 2.615 2.643 2.498 2.682 2.563 2.637 2.968 2.937 2.963 5.098 5.672 4.897 5.624 5.269 5.415 5.483 2.663 70.606 11.319 13.730 34.971 33.939 40.722 37.870 23.079 24.682 24.188 18.453 21.172 19.527 10.604 LOD 0.0036 0.0120 0.0790 0.0073 0.0140 0.4066 0.3551 0.0936 0.8207 0.0307 0.0695 0.0420 0.0931 0.0354 0.0709 %Rec 99.4 104.6 105.7 99.9 107.3 102.5 105.5 118.7 117.5 118.5 102.0 113.4 97.9 112.5 105.4 108.3 109.7 98.6 98.1 94.3 114.4 97.1 94.3 113.1 105.2 96.2 102.8 100.8 76.9 88.2 81.4 106.0 S/N 32 195 167 182 362 566 107 159 245 490 187 193 232 0 63 62 59 280 2708 4719 2948 274 321 1330 0 88 2646 986 1222 746 2203 449 Noise 484 118 248 209 109 15 404 344 299 199 61 36 42 0 54 119 106 28 354 64 143 182 130 41 0 1179 106 321 259 947 34 261 Height 15643 23136 41366 38052 39652 8737 43376 54598 73464 97770 11436 6968 9663 5226 3402 7396 6320 7858 957565 301233 420901 49991 41714 54779 34475 103637 279250 316249 315918 706856 74369 117336 IS Height 103637 279250 279250 279250 957565 957565 316249 315918 315918 706856 54779 34475 54779 34475 54779 49991 41714 74369 420901 420901 420901 420901 420901 420901 420901 420901 420901 420901 420901 420901 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 184 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-C,,1/2 Name: FC3G_013S006 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:54:43 PFBA FC3G 013S006 PFPeA FC3G 013S006 PFHxA FC3G 013S006 PFHpA FC3G 013S006 PFOA-1 FC3G 013S006 PFOA-2 FC3G 013S006 PFNA FC3G 013S006 PFHxS-1 FC3G 013S006 min PFDA FC3G 013S006 min PFHxS-2 FC3G 013S006 min PFUnA FC3G 013S006 min PFHxS-3 FC3G 013S006 min min PFDoA FC3G_013S006 100- 9.27 5.75e4 247552 %- PFBS-1 FC3G 013S006 min min 8.00 10.00 12.00 PFOS-1 FC3G 013S006 PFOS-2 FC3G 013S006 min PFBS-2 FC3G 013S006 min PFOSA FC3G 013S006 min min min min min min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 185 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-C,,1/2 Name: FC3G_013S006 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:54:43 13C2-PFOA FC3G 013S006 100- 7.14 2.12e5 829784 %- 13C8-PFOA FC3G 013S006 100- 7.14 6.89e4 265043 %- 13C4-PFOA FC3G_013S006 7.14 100 9.11e4 ,3 5 8 2 4 2 % 13C4-PFOS-1 FC3G 013S006 07.00 T-r min 8.00 18O2-PFHxS-2 FC3G 013S006 07.00 13C4-PFBA FC3G 013S006 T-r min 8.00 0 7.00 8.00 min 13C2-PFHxA FC3G 013S006 13C5-PFNA FC3G 013S006 13C4-PFOS-2 FC3G 013S006 min 13C2-PFDA FC3G 013S006 18O2-PFHxS-1 FC3G 013S006 min 13C2-PFDoA FC3G 013S006 min min min min min min min 13C8-PFOSA FC3G_013S006 9.96 1001 .44e4 71206 %- 13C2-PFOUEA FC3G 013S006 8.00 10.00 min 12.00 min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 186 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-C,,1/2 Name: FC3G_013S006 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:54:43 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 > 218.... 5.99 27 312.97> 268.98 6.38 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.64 30 612.95 > 568.... 9.27 24 298.94> 79.96 6.51 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.38 24 398.84> 118.70 7.33 22 498.93> 79.96 8.41 23 498.93> 98.96 8.41 31 498 > 78 9.99 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.28 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.96 458.98 >393.98 7.43 Area 10366 14550 27425 28576 29956 6254 32114 42575 47553 57475 7781 4065 7449 3685 2264 6349 4931 5955 212071 68945 91100 10194 8158 11291 7204 22826 58083 67753 84230 142436 14395 26306 IS Area 22826 58083 58083 58083 212071 212071 67753 84230 84230 142436 11291 7204 11291 7204 11291 10194 8158 14395 91100 91100 91100 91100 91100 91100 91100 91100 91100 91100 91100 91100 ng/g 8.463 8.299 8.875 8.430 9.230 8.626 9.770 9.497 8.941 10.256 18.060 18.249 17.890 17.091 16.164 16.969 19.539 8.891 72.985 12.118 11.758 35.015 34.368 39.522 37.913 24.474 26.055 22.628 21.046 21.564 23.281 9.304 LOD 0.0043 0.2197 0.0627 0.0127 0.3071 0.2073 0.2804 0.1568 0.0509 0.5747 0.0743 0.0379 0.0721 0.0790 0.0762 0.0405 %Rec 84.6 83.0 88.8 84.3 92.3 86.3 97.7 95.0 89.4 102.6 90.3 91.2 89.4 85.5 80.8 84.8 97.7 87.2 101.4 101.0 98.0 97.3 95.5 109.8 105.3 102.0 108.6 94.3 87.7 89.8 97.0 93.0 S/N 189 596 598 692 487 385 212 465 1332 876 768 514 179 0 0 108 176 535 3477 2795 115 532 376 770 2225 130 1262 1626 817 874 1154 688 Noise 213 118 208 169 246 58 564 329 162 283 47 36 150 0 0 178 98 41 239 95 3119 79 87 59 13 703 217 149 378 684 62 144 Height 40206 70334 124363 117134 120024 22218 119633 152978 216315 247552 35802 18662 26771 14483 8599 19312 17328 21789 829784 265043 358242 42111 32767 45248 28229 91558 273883 241529 308913 597777 71206 99136 IS Height 91558 273883 273883 273883 829784 829784 241529 308913 308913 597777 45248 28229 45248 28229 45248 42111 32767 71206 358242 358242 358242 358242 358242 358242 358242 358242 358242 358242 358242 358242 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 187 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S007 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:11:44 PFBA FC3G 013S007 PFPeA FC3G 013S007 PFHxA FC3G 013S007 PFHpA FC3G 013S007 PFOA-1 FC3G 013S007 PFOA-2 FC3G 013S007 PFNA FC3G 013S007 min PFDA FC3G 013S007 min PFUnA FC3G 013S007 min PFDoA FC3G 013S007 min PFBS-1 FC3G 013S007 min PFBS-2 FC3G 013S007 min PFHxS-1 FC3G 013S007 min PFHxS-2 FC3G 013S007 min PFHxS-3 FC3G 013S007 min PFOS-1 FC3G 013S007 min PFOS-2 FC3G 013S007 min PFOSA FC3G 013S007 min min min min min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 188 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S007 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:11:44 13C2-PFOA FC3G 013S007 100- 7.14 2.57e5 994066 %- \ 13C8-PFOA FC3G 013S007 100- 7.14 8.15e4 315323 %- 13C4-PFOA FC3G_013S007 7.14 100 .17e5 444231 % 13C4-PFOS-1 FC3G_013S007 8.41 100 1.12e4 13C4-PFOS-2 FC3G_013S007 100- 8.41 1.01e4 41336 % 18O2-PFHxS-1 FC3G 013S007 0 - V i T-r min 7.00 8.00 07.00 t t min 8.00 0 7.00 8.00 min 8.00 10.00 min 12.00 0 8.00 10.00 min 12.00 18O2-PFHxS-2 FC3G 013S007 13C4-PFBA FC3G 013S007 13C2-PFHxA FC3G 013S007 13C5-PFNA FC3G 013S007 13C2-PFDA FC3G 013S007 13C2-PFDoA FC3G 013S007 min min min min min min min 13C8-PFOSA FC3G_013S007 9.96 1001.61e4 83206 %- 13C2-PFOUEA FC3G 013S007 0 8.00 10.00 min 12.00 min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 189 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S007 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:11:44 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 > 218.... 5.99 27 312.97> 268.98 6.36 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.61 30 612.95 > 568.... 9.24 24 298.94> 79.96 6.51 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.41 23 498.93> 98.96 8.41 31 498 > 78 9.96 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.24 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.96 458.98 >393.98 7.43 Area 45779 67138 130876 146028 146755 33767 148161 190115 234886 286387 37888 20644 35921 19357 12138 31778 21613 28377 256975 81521 116856 11195 10080 13066 8344 23689 62914 83045 93912 174079 16141 30462 IS Area 23689 62914 62914 62914 256975 256975 83045 93912 93912 174079 13066 8344 13066 8344 13066 11195 10080 16141 116856 116856 116856 116856 116856 116856 116856 116856 116856 116856 116856 116856 ng/g 39.474 35.482 40.497 40.341 38.027 38.698 37.217 39.447 40.418 44.003 76.343 83.291 74.702 79.505 77.034 79.662 71.699 38.844 68.946 11.171 15.082 29.976 33.108 35.656 34.234 19.801 22.002 21.623 18.293 20.546 20.351 10.774 LOD 0.0261 0.2270 0.1114 0.0096 0.1470 0.2068 0.1372 0.1351 0.2271 0.5986 0.0550 0.0300 0.0624 0.1144 0.0503 0.0108 %Rec 98.7 88.7 101.2 100.9 95.1 96.7 93.0 98.6 101.0 110.0 95.4 104.1 93.4 99.4 96.3 99.6 89.6 97.1 95.8 93.1 125.7 83.3 92.0 99.0 95.1 82.5 91.7 90.1 76.2 85.6 84.8 107.7 S/N 460 1692 1704 2276 6798 0 1221 1408 4601 3927 9176 3296 766 0 0 355 745 1329 1889 3552 308 460 781 824 464 98 1397 2215 844 625 1646 3001 Noise 364 181 337 240 88 0 475 487 215 303 18 29 176 0 0 292 101 79 526 89 1443 98 53 63 70 907 199 146 406 1229 51 39 Height 167582 305855 574333 547200 597366 137348 580471 685265 989187 1189540 166672 94814 134378 71137 44761 103681 75105 105037 994066 315323 444231 44971 41336 51668 32517 88618 278315 322936 342479 767396 83206 117556 IS Height 88618 278315 278315 278315 994066 994066 322936 342479 342479 767396 51668 32517 51668 32517 51668 44971 41336 83206 444231 444231 444231 444231 444231 444231 444231 444231 444231 444231 444231 444231 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 190 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-E,,1/2 Name: FC3G_013S008 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:28:37 PFBA FC3G 013S008 PFPeA FC3G 013S008 PFHxA FC3G 013S008 PFHpA FC3G 013S008 PFOA-1 FC3G 013S008 PFOA-2 FC3G 013S008 min min min min min PFNA FC3G 013S008 7.55 1007.91e5 2958915 %- J 07.00 PFHxS-1 FC3G 013S008 8.00 min PFDA FC3G_013S008 8.05 100 1 .04e6 3722971 % 0 8.00 10.00 min 12.00 PFHxS-2 FC3G 013S008 PFUnA FC3G 013S008 PFHxS-3 FC3G 013S008 100- 7.33 5.69e4 201711 %- PFDoA FC3G 013S008 min PFOS-1 FC3G 100- 013S008 8.35 1 .6 5 e 5 481251 %- PFBS-1 FC3G 013S008 min PFOS-2 FC3G_013S008 8.41 100 1 .08e5 362848 % PFBS-2 FC3G 013S008 min PFOSA FC3G 013S008 min min 0 - t min 0 - V 9.96 min 0 min 7.00 8.00 8.00 10.00 12.00 8.00 10.00 12.00 min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 191 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-E,,1/2 Name: FC3G_013S008 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:28:37 13C2-PFOA FC3G 013S008 100%0- 7.14 2.20e5 851947 \ J V, T-r min 7.00 8.00 13C8-PFOA FC3G 013S008 100%0- 7.14 7.29e4 286060 \ J =rr min 7.00 8.00 13C4-PFOA FC3G_013S008 7.09 100 , 9.58e4 392720 % 0 7.00 8.00 min 13C4-PFOS-1 FC3G 013S008 18O2-PFHxS-2 FC3G 013S008 13C4-PFBA FC3G 013S008 13C2-PFHxA FC3G 013S008 13C5-PFNA FC3G 013S008 13C4-PFOS-2 FC3G 013S008 min 13C2-PFDA FC3G 013S008 18O2-PFHxS-1 FC3G 013S008 min 13C2-PFDoA FC3G 013S008 min min min min min min min 13C8-PFOSA FC3G 013S008 13C2-PFOUEA FC3G 013S008 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 192 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-E,,1/2 Name: FC3G_013S008 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:28:37 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.24 27 262.98 > 218.... 5.97 27 312.97> 268.98 6.34 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.61 30 612.95 > 568.... 9.24 24 298.94> 79.96 6.49 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.38 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.41 31 498 > 78 9.99 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.09 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.26 21 314.97>269.98 6.34 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.96 458.98 >393.98 7.43 Area 255608 336833 610449 638000 720547 163932 791016 1042945 1347697 1640496 178976 93640 174989 86452 56878 164663 108199 177335 219864 72926 95773 11328 9161 11125 6980 24224 55802 74331 96533 191720 18940 30315 IS Area 24224 55802 55802 55802 219864 219864 74331 96533 96533 191720 11125 6980 11125 6980 11125 11328 9161 18940 95773 95773 95773 95773 95773 95773 95773 95773 95773 95773 95773 95773 ng/g 220.277 200.881 214.713 199.318 219.330 219.931 222.788 212.560 226.688 231.852 424.077 455.894 427.668 426.879 426.669 410.612 399.187 208.283 71.975 12.193 12.361 37.010 36.713 37.040 34.945 24.706 23.811 23.614 22.943 27.609 29.138 10.722 LOD 4.1083 0.0341 0.0399 0.1185 0.0741 0.0076 0.0947 0.1511 0.2966 0.0206 0.1154 0.5904 0.0717 0.0236 0.0635 0.0819 0.0753 0.0491 %Rec 110.1 100.4 107.4 99.7 109.7 110.0 111.4 106.3 113.3 115.9 106.0 114.0 106.9 106.7 106.7 102.7 99.8 104.1 100.0 101.6 103.0 102.8 102.0 102.9 97.1 102.9 99.2 98.4 95.6 115.0 121.4 107.2 S/N 124 7323 6667 10287 41534 0 3532 6927 6793 11958 16927 4810 6713 0 0 1797 1585 5818 2754 4603 392 739 334 5201 823 115 1189 2591 946 1044 1366 656 Noise 7455 199 440 244 69 0 838 537 856 584 45 83 91 0 0 268 229 113 309 62 1003 63 101 8 32 791 230 102 365 777 67 169 Height 926163 1459708 2936564 2505653 2873672 652687 2958915 3722971 5811819 6980212 756561 399067 611636 288492 201711 481251 362848 655614 851947 286060 392720 46719 33762 44082 25941 90727 273066 263093 345133 811346 91462 110888 IS Height 90727 273066 273066 273066 851947 851947 263093 345133 345133 811346 44082 25941 44082 25941 44082 46719 33762 91462 392720 392720 392720 392720 392720 392720 392720 392720 392720 392720 392720 392720 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 193 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-F,,1/2 Name: FC3G_013S009 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:45:38 PFBA FC3G 013S009 PFPeA FC3G 013S009 PFHxA FC3G 013S009 PFHpA FC3G 013S009 PFOA-1 FC3G 013S009 PFOA-2 FC3G 013S009 min min PFNA FC3G 013S009 7.55 1001 .5 0 e 6 5454400 %- PFDA FC3G_013S009 8.05 100 2.12e6 '5 9 1 8 5 9 % PFUnA FC3G 013S009 07.00 8.00 min PFHxS-1 FC3G 100- 013S009 7.38 3.21e5 1086340 %- 0 8.00 10.00 min 12.00 PFHxS-2 FC3G_013S009 7.38 _ 100 1 .83e5 643061 % PFHxS-3 FC3G 013S009 07.00 8.00 min 0 7.00 8.00 min min PFDoA FC3G 013S009 min PFOS-1 FC3G 013S009 min min PFBS-1 FC3G 013S009 min PFOS-2 FC3G 013S009 min min PFBS-2 FC3G 013S009 min min min PFOSA FC3G_013S009 9.99 1 00 - 3.65e5 1349392 %- min 0 min 8.00 10.00 12.00 PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 194 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-F,,1/2 Name: FC3G_013S009 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:45:38 13C2-PFOA FC3G 013S009 100- 7.14 2.19e5 875102 %- J 07.00 =n min 8.00 13C8-PFOA FC3G 013S009 100- 7.14 7.14e4 286636 %- 07.00 T-r min 8.00 13C4-PFOA FC3G_013S009 7.14 100 9.27e4 362029 % 0 7.00 8.00 min 13C4-PFOS-1 FC3G 013S009 18O2-PFHxS-2 FC3G 013S009 13C4-PFBA FC3G 013S009 13C2-PFHxA FC3G 013S009 13C5-PFNA FC3G 013S009 13C4-PFOS-2 FC3G 013S009 min 13C2-PFDA FC3G 013S009 18O2-PFHxS-1 FC3G 013S009 min 13C2-PFDoA FC3G 013S009 min min min min min min min 13C8-PFOSA FC3G 013S009 13C2-PFOUEA FC3G 013S009 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 195 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-F,,1/2 Name: FC3G_013S009 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:45:38 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.24 27 262.98 > 218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.78 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.64 30 612.95 > 568.... 9.27 24 298.94> 79.96 6.51 25 298.94 > 98.95 6.51 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.38 24 398.84> 118.70 7.38 22 498.93> 79.96 8.41 23 498.93> 98.96 8.41 31 498 > 78 9.99 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.26 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.99 458.98 >393.98 7.43 Area 529256 733203 1180792 1404129 1356679 315010 1497967 2117893 2783679 3481032 333040 176912 321443 183159 102356 339460 241234 364640 218928 71423 92721 12244 9863 9932 7888 26173 52550 81811 102880 214405 19590 30111 IS Area 26173 52550 52550 52550 218928 218928 81811 102880 102880 214405 9932 7888 9932 7888 9932 12244 9863 19590 92721 92721 92721 92721 92721 92721 92721 92721 92721 92721 92721 92721 ng/g 423.109 464.376 441.448 466.012 414.938 424.491 383.440 405.438 439.560 440.561 884.007 762.814 879.991 800.785 860.628 783.790 827.661 414.386 74.028 12.334 11.967 41.318 40.828 34.157 40.790 27.572 23.162 26.846 25.256 31.892 31.130 10.650 LOD 0.0796 0.0587 0.7288 0.0721 0.0095 0.0278 0.1918 0.1934 0.0762 0.0530 0.3357 0.0462 0.0367 0.0757 0.0925 0.0647 0.0119 %Rec 105.8 116.1 110.4 116.5 103.7 106.1 95.9 101.4 109.9 110.1 110.5 95.4 110.0 100.1 107.6 98.0 103.5 103.6 102.8 102.8 99.7 114.8 113.4 94.9 113.3 114.9 96.5 111.9 105.2 132.9 129.7 106.5 S/N 1863 10162 20565 25207 25056 0 5263 8285 21784 4243 47040 15977 3256 0 0 4253 2892 3875 3151 4024 1290 654 628 1211 2137 230 1905 2045 932 1103 1835 2674 Noise 983 308 269 209 225 0 1036 916 519 3422 29 44 334 0 0 236 289 348 278 71 281 74 61 29 13 415 136 146 402 809 53 45 Height IS Height 1830687 95441 3127445 259723 5537483 259723 5256633 259723 5647306 875102 1287049 875102 5454400 297518 7591859 374167 11302019 374167 14521640 892691 1386435 34943 697209 28524 1086340 34943 643061 28524 326226 34943 1004414 48411 835254 38196 1349392 97227 875102 362029 286636 362029 362029 48411 362029 38196 362029 34943 362029 28524 362029 95441 362029 259723 362029 297518 362029 374167 362029 892691 362029 97227 362029 121600 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 196 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-G,,1/2 Name: FC3G_013S010 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:02:39 PFBA FC3G 013S010 PFPeA FC3G 013S010 PFHxA FC3G 013S010 PFHpA FC3G 013S010 PFOA-1 FC3G 013S010 PFOA-2 FC3G 013S010 min min min min min PFNA FC3G 013S010 PFDA FC3G 013S010 PFUnA FC3G 013S010 PFDoA FC3G_013S010 100- 9.24 8.08e6 31703496 %- PFBS-1 FC3G 013S010 PFBS-2 FC3G 013S010 min min 0 min min 8.00 10.00 12.00 PFHxS-1 FC3G 013S010 \1 0 0 %- A PFHxS-2 FC3G 100- 013S010 7.33 3.46e5 1088404 %- PFHxS-3 FC3G 013S010 100%- 7.33 \2 .0 8 e 5 677773 PFOS-1 FC3G 100- 013S010 8.35 7.45e5 1936700 1% - PFOS-2 FC3G_013S010 8.35 100 5.03e5 1540466 % PFOSA FC3G 013S010 07.00 8.00 min 07.00 8.00 min / 0- 7.00 V +. t min 8.00 08.00 9.99 10.00 min 12.00 0 8.00 10.00 min 12.00 min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 197 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-G,,1/2 Name: FC3G_013S010 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:02:39 13C2-PFOA FC3G 013S010 100- 7.09 1.78e5 701478 \% - J Vj 0- -TT min 7.00 8.00 13C8-PFOA FC3G 013S010 100- 7.09 5.80e4 235720 %- 07.00 -TT min 8.00 13C4-PFOA FC3G_013S010 7.09 100 , 7.63e4 309066 % 0 7.00 8.00 min 13C4-PFOS-1 FC3G 013S010 18O2-PFHxS-2 FC3G 013S010 13C4-PFBA FC3G 013S010 13C2-PFHxA FC3G 013S010 13C5-PFNA FC3G 013S010 13C4-PFOS-2 FC3G 013S010 min 13C2-PFDA FC3G 013S010 18O2-PFHxS-1 FC3G 013S010 min 13C2-PFDoA FC3G 013S010 min min min min min min min 13C8-PFOSA FC3G_013S010 9.99 _ 1 0 0 - 1.98e4 94630 %- 13C2-PFOUEA FC3G 013S010 0 8.00 10.00 min 12.00 min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 198 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-G,,1/2 Name: FC3G_013S010 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:02:39 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.21 27 262.98 > 218.... 5.95 27 312.97> 268.98 6.34 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.61 30 612.95 > 568.... 9.24 24 298.94> 79.96 6.49 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.23 21 314.97>269.98 6.34 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.99 458.98 >393.98 7.43 Area 1106351 1451118 2301871 2756213 2785893 654423 3206580 4358040 6297988 8082436 616948 325842 630934 345834 208209 744593 502724 935573 178447 57989 76343 9275 8107 7971 5514 21823 43564 64985 98693 246180 19776 26510 IS Area 21823 43564 43564 43564 178447 178447 64985 98693 98693 246180 7971 5514 7971 5514 7971 9275 8107 19776 76343 76343 76343 76343 76343 76343 76343 76343 76343 76343 76343 76343 ng/g 1062.375 1108.718 1038.652 1103.663 1045.711 1082.038 1033.584 870.216 1037.007 891.613 2040.651 2011.315 2152.301 2163.792 2182.301 2270.578 2099.973 1053.749 73.284 12.163 9.853 38.017 40.756 33.293 34.633 27.921 23.320 25.900 29.426 44.474 38.165 9.376 LOD 0.0056 0.0375 0.0797 1.0195 0.0788 0.2471 0.4177 0.1015 0.0101 0.0962 0.1915 0.2888 0.1262 0.0546 0.5500 0.1083 0.0517 0.0657 0.0939 0.0603 0.0734 %Rec 106.2 110.9 103.9 110.4 104.6 108.2 103.4 87.0 103.7 89.2 102.0 100.6 107.6 108.2 109.1 113.5 105.0 105.4 101.8 101.4 82.1 105.6 113.2 92.5 96.2 116.3 97.2 107.9 122.6 185.3 159.0 93.8 S/N 18584 63240 39460 73556 11748 0 13098 1730 15999 37235 35111 36917 19148 0 77975 4771 5738 9816 2103 3619 307 510 378 689 1550 129 770 1284 1190 1399 2244 383 Noise 204 93 280 140 947 0 857 8898 1581 851 67 32 102 0 9 406 268 336 334 65 1006 63 78 41 12 580 273 175 297 701 42 269 Height IS Height 3791494 74558 5903201 210376 11029682 210376 10302533 210376 11127175 701478 2625699 701478 11230852 224568 15396270 353488 25290048 353488 31703496 980671 2341614 28081 1197156 18186 1959121 28081 1088404 18186 677773 28081 1936700 32186 1540466 29278 3299352 94630 701478 309066 235720 309066 309066 32186 309066 29278 309066 28081 309066 18186 309066 74558 309066 210376 309066 224568 309066 353488 309066 980671 309066 94630 309066 103032 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMX 1.0 MMX 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 199 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-H,,1/2 Name: FC3G_013S011 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:19:32 PFBA FC3G 013S011 PFPeA FC3G 013S011 PFHxA FC3G 013S011 PFHpA FC3G 013S011 PFOA-1 FC3G 013S011 PFOA-2 FC3G 013S011 PFNA FC3G 013S011 PFHxS-1 FC3G 013S011 min PFDA FC3G 013S011 min PFHxS-2 FC3G 013S011 min min PFUnA FC3G 013S011 min PFHxS-3 FC3G 013S011 min min min min PFDoA FC3G_013S011 100- 9.24 1 .40e7 49788828 %- PFBS-1 FC3G 013S011 PFBS-2 FC3G 013S011 min 0 min 8.00 10.00 12.00 PFOS-1 FC3G 100- 013S011 8.35 r 1.07e6 2454084 %- PFOS-2 FC3G_013S011 8.35 100 7.49e5 2091331 % min PFOSA FC3G 013S011 Vi 9.99 min 0 - 8.00 10.00 12.00 0 8.00 10.00 min 12.00 min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 200 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-H,,1/2 Name: FC3G_013S011 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:19:32 13C2-PFOA FC3G 013S011 100%0- 7.09 1 .3 3 e 5 549876 \ JV -n 7.00 8.00 min 13C8-PFOA FC3G 013S011 100%0- 7.09 4.13e4 165812 \ J = n min 7.00 8.00 13C4-PFOA FC3G_013S011 7.09 100 5.42e4 1220898 % 0 7.00 8.00 min 13C4-PFOS-1 FC3G 013S011 18O2-PFHxS-2 FC3G 013S011 13C4-PFBA FC3G 013S011 13C2-PFHxA FC3G 013S011 13C5-PFNA FC3G 013S011 13C4-PFOS-2 FC3G 013S011 min 13C2-PFDA FC3G 013S011 18O2-PFHxS-1 FC3G 013S011 min 13C2-PFDoA FC3G 013S011 min min min min min min min 13C8-PFOSA FC3G 013S011 13C2-PFOUEA FC3G 013S011 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 201 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-H,,1/2 Name: FC3G_013S011 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:19:32 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 > 218.... 5.97 27 312.97> 268.98 6.34 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 > 418.... 7.49 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.61 30 612.95 > 568.... 9.24 24 298.94> 79.96 6.47 25 298.94 > 98.95 6.47 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.41 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.24 21 314.97>269.98 6.33 21 467.96>422.97 7.49 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.21 21 506>78 9.99 458.98 >393.98 7.38 Area IS Area 1451154 13033 2412291 33387 3564080 33387 4155998 33387 4425141 132546 1008404 132546 4992039 44026 6485407 85123 9320023 85123 14006239 246757 910658 5135 464895 3683 875468 5135 486835 3683 289888 5135 1072351 6391 748686 5168 1753377 15345 132546 54200 41290 54200 54200 6391 54200 5168 54200 5135 54200 3683 54200 13033 54200 33387 54200 44026 54200 85123 54200 246757 54200 15345 54200 19906 ng/g 2334.523 2404.932 2098.793 2171.579 2236.502 2244.799 2375.365 1501.794 1779.414 1542.003 4675.857 4298.276 4635.939 4561.884 4717.185 4746.605 4907.534 2545.530 76.671 12.198 6.996 36.896 36.593 30.210 32.577 23.488 25.174 24.714 35.749 62.790 41.713 7.040 LOD 0.3175 0.1590 0.4566 0.0846 0.0536 0.4240 2.6270 3.0748 2.8822 0.1097 0.0294 0.0292 0.2022 0.2100 0.6443 0.1409 1.0759 0.0599 0.0437 0.1550 0.0834 0.1188 0.0522 %Rec 93.4 96.2 84.0 86.9 89.5 89.8 95.0 60.1 71.2 61.7 93.5 86.0 92.7 91.2 94.3 94.9 98.2 101.8 106.5 101.7 58.3 102.5 101.6 83.9 90.5 97.9 104.9 103.0 149.0 261.6 173.8 70.4 S/N 4954 30997 45309 34263 9648 0 29400 10240 19203 81743 31464 27854 4897 0 0 2634 3303 24758 2134 1227 718 446 418 111 525 56 1475 1468 597 2038 1157 405 Noise 995 315 383 450 1889 0 605 2228 1777 609 111 60 542 0 0 932 633 230 258 135 308 48 40 149 22 811 108 106 501 446 59 182 Height IS Height 4931902 45394 9763751 159099 17371712 159099 15421288 159099 18224222 549876 4135016 549876 17796560 155374 22815656 299509 34117992 299509 49788828 907986 3500984 16511 1658313 11370 2653335 16511 1450628 11370 863093 16511 2454084 21217 2091331 16834 5702633 68691 549876 220898 165812 220898 220898 21217 220898 16834 220898 16511 220898 11370 220898 45394 220898 159099 220898 155374 220898 299509 220898 907986 220898 68691 220898 73576 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMX 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMX 1.0 MMX 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 202 of 234 www.axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time Method: G:\M asslynx\Master M ethods\PFC\FC_043_08_02.m db 06 Nov 2012 13:32:38 Calibration: G:\M asslynx\lnst_G\PFC.PRO \CurveDB\FC3G_013-A1.cdb 14 Ja n 2013 13:08:53 ID: FC-LIN -42151T-D,,1/2 Name: FC3G_013S014 Description: 1,,15uL Date: 11>Jan-2013 Tim e: 16:10:30 PFBA FC3G_013S014 100-, 5.23 4.10e4 155020 I 5.00 6.00 7.00 PFNA FC3G_013S014 100-, 7.49 1.41 e5 533591 f l %- 7.00 8.00 P F H xS -1 FC3G_013S014 100- _ 7.33 , 3.33e4 \ 126745 %- PFPeA FC3G_013S014 100- 5.97 6.42e4 i| 287375 i: %" i! 5.00 6.00 7.00 PFHxA FC3GJ313S014 100- 6.36 1.24e5 555146 % 1 xMi W " min 5.00 6.00 7.00 PFHpA FC3GJ313S014 100 6.73 1 .3 2 e 5 l 519650 I %- 5.00 6.00 7.00 PFDA FC3G 013S014 100- 8.05 1.90e5 1 700937 %- M PFUnA FC3G_013S014 100 8.58 2.30e5 :| 998229 % I 1 PFDoA FC3G 013S014 100- 9.21 2.57e5 1071677 % i - -- mmiinn 8.00 10.00 12.00 o8.00 10.00 12.00 8.00 10.00 12.00 P FH xS -2 FC3G_013S014 100' 1- 1 7.33 . 9464^1 ` 74496 1 %- P F H xS -3 FC3G_013S014 100- 7.33 1.14e4 A 41849 \ %- P FO S-1 FC3G_013S014 11f.0nn I" 380.355e4 1 89546 %- 7.00 8.00 7.00 8.00 7.00 8.00 o4fl 8.00 9.99 ' T 11I j 11 11I ~ I 10.00 12.00 PFOA-1 FC3G__013S014 100- 7.09 ; 1.37e5 520722 %- 7.00 8.00 PFBS-1 FC3G_013S014 100- 6.49 3 .4 8 e 4 l 1495677 Ii %- J 5.00 6.00 7.00 PFO S-2 FC3G__013S014 100- __ 8.35 2.05e4 69582 %- | 8.00 10.00 12.00 P FO A -2 FC3G 013S014 P F B S -2 FC3G_013S014 100- 6.47 _ 1.84e4 81465 %- 5.00 6.00 7.00 PFO SA FC3G_013S014 100- 9.99 2.92e4 ~| 110473 I %- _ 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. Page 203 of 234 www.axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnsM3\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: FC-U N-42151T-D,,1/2 Name: FC3G_013S014 Description: 1,,15uL Date: 11-Jan-2013 Tim e: 16:10:30 o ooI 1 13C 2 -P FO A FC3G 013S014 100- 7.09 2.33e5 908268 %z 0- 74- . . n - -- min 7.00 8.00 13C 8 -P FO A FC3G 013S014 7.09 K 7 87e4 , ,303582 %- 0J _ 7.00 -T-r min 8.00 1 3 C 4 -P FO A FC3G 013S014 100q 7.09 i f 9.38e4 366684 %- 0^ 7.00 8.00 1 8 0 2 -P FH X S -2 FC3G_013S014 1007 7.7477e7 3- 29494 . %- i i n min 7.00 8.00 13C4-PFBA FC3G 013S014 11U0U0- 2.22e4 83612 %4 28 0 nTn-mTFFFtA^ffR'nTTTrf min 5.00 6.00 7.00 1 3 C 2 -P F H x A FC3G_013S014 6.36 100J %- 0~ 5.00 6.00 7.00 13C8-PFO SA FC3G_013S014 100- 9.99 1.60e4~| 80297 /r 1 3 C 2 -P FO U E A FC3G 013S014 7.38 3.13e4 119413 o- min 8.00 10.00 12.00 0" 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S014 100- 8.35 f 1.06e4 M 40785 Li a%H il 8.00 10.00 12.00 13C5-PFNA FC3G 013S014 1 3C 4-P F O S -2 FC3G 013S014 13C2-PFDA FC3G 013S014 1802-P F H xS -1 FC3G013S014 100- 7.33 -1.07e4 ' 42422 I % 7.00 8.00 13C2-PFDOA FC3G_013S014 100- 9.21 1.53e5 645665 8.00 ........ I IMIIN i 10.00 12.00 Page 204 of 234 www.axysanalytical.com Quantify Sam ple Report M a ssL y n x 4.1 S C N 6 2 7 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: FC-LIN -42151T-D,,1/2 Name: FC3G_013S014 Description: 1,,15uL Date: 11-Jan-2013 Tim e: 16:10:30 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.23 27 262.98 >218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.49 29 512.96 >468.... 8.05 29 562.96 >518.... 8.58 30 612.95 >568.... 9.21 24 298.94> 79.96 6.49 25 298.94 > 98.95 6.47 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.24 21 314.97>269.98 6.36 21 467.96>422.97 7.49 21 514.96 >469.96 8.05 21 614.95 >569.... 9.21 21 506>78 9.99 458.98 >393.98 7.38 Area 40975 64184 123830 132304 137068 33833 140845 190499 229964 257323 34760 18417 33292 19432 11407 30529 20510 29207 233423 78698 93820 10614 9188 10732 7467 22226 59287 79295 90207 152994 15953 31291 IS Area 22226 59287 59287 59287 233423 233423 79295 90207 90207 152994 10732 7467 10732 7467 10732 10614 9188 15953 93820 93820 93820 93820 93820 93820 93820 93820 93820 93820 93820 93820 ng/fl 37.609 35.996 40.663 38.780 39.107 42.693 37.052 41.170 41.201 45.002 85.290 83.028 84.302 89.255 88.235 80.728 74.688 40.467 78.004 13.432 12.109 35.398 37.586 36.475 38.158 23.140 25.824 25.715 21.886 22.491 25.052 11.067 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0568 0.2160 0.0662 0.0241 0.0505 0.0977 0.2038 0.1160 0.2353 0.0969 0.6682 0.0312 0.0189 0.2417 0.1008 0.0827 0.0428 %Rec 94.0 90.0 101.7 96.9 97.8 106.7 92.6 102.9 103.0 112.5 106.6 103.8 105.4 111.6 110.3 100.9 93.4 101.2 108.3 111.9 100.9 98.3 104.4 101.3 106.0 96.4 107.6 107.1 91.2 93.7 104.4 110.7 S/N 200 1130 1505 1956 8223 441 3613 1970 2104 2106 5491 1886 2096 0 0 851 488 1634 9649 788 372 512 968 470 1194 100 2847 3924 245 723 1171 776 Noise 776 254 369 266 63 298 148 356 474 509 27 43 60 0 0 105 142 68 94 385 986 80 37 90 25 836 93 76 1298 893 69 154 H e ig h t 155020 287375 555146 519650 520722 131702 533591 700937 998229 1071677 149567 81465 126745 74496 41849 89546 69582 110473 908268 303582 366684 40785 35760 42422 29494 83612 265916 298638 317323 645665 80297 119413 IS Height Sam ple size 83612 1.0000 265916 1.0000 265916 1.0000 265916 1.0000 908268 1.0000 908268 1.0000 298638 1.0000 317323 1.0000 317323 1.0000 645665 1.0000 42422 1.0000 29494 1.0000 42422 1.0000 29494 1.0000 42422 1.0000 40785 1.0000 35760 1.0000 80297 1.0000 366684 1.0000 366684 1.0000 366684 1.0000 1.0000 366684 1.0000 366684 1.0000 366684 1.0000 366684 366684 1.0000 1.0000 366684 366684 366684 366684 1.0000 1.0000 1.0000 1.0000 1.0000 Factors Detection... 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb m Page 205 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 D ataset: G :\M asslynx\lnstJ3\PFC .PRO \FC \FC3G _013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S038 Description: 1,,15uL Date: 11 ^Jan-2013 Time: 22:57:42 PFBA FC3G_013S038 100 4.68e4 i 174507 : -W - 5.00 -HTpii,: 'tt min 6.00 7.00 PFNA FC3G 013S038 PFPeA FC3G 013S038 100- 6 .9 0 e 4 l 308459 o: ............. 111r11111 wrp tt min 5.00 6.00 7.00 PFDA FC3GJ013SO38 100 7.99 f 1.97e5 ' 714684 %- PFHxA FC3G_013S038 100-1 6.33 1.37e5 631423 %J 0J rArn 5.00 6.00 7.00 PFUnA FC3G_013S038 100- 8.55 2.50e5 1060816 %- 0 ^L . . . . 8.00 10.00 12.00 .... 8.00 10.00 12.00 PFHxS-1 FC3G 013S038 100- 7.29 3.19e4 119600 %- o-J 7.00 -T min 8.00 PFHxS-2 FC3G 013S038 100- 7.29 ' 1.73e4 62998 J ill 0-4 - r ~ ~ . r4 min 7.00 8.00 PFHxS-3 FC3G 013S038 100- 7.29 1.02e4 - : 35856 %Z 07.00 min 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S038 100- 6.68 1.38e5 548581 I %- ijn 11,11 11-itj11Ai yj r 5.00 6.00 7.00 PFDoA FC3GJJ13S038 100-, 9.15 2.44e5 976177 %- 8.00 10.00 12.00 PFOS-1 FC3G 013S038 PFOA-1 FC3G_013S038 100- 7.09 A 1 44e5 ' 549281 PFOA-2 FC3G_013S038 O--M - , --i 7.00 i.. 8.00 PFBS-1 FC3G 013S038 PFBS-2 FC3G_013S038 100- 6.44 1 ,8 5 e 4 84958 %- P F O S -2 FC3G_013S038 100- 8.29 i " 1.98e4 I 66270 %- min 0- -TTTl 5.00 6.00 7.00 PFOSA FC3GJ313S038 100 9.96 2.70e4 J 97741 %- 8.00 10.00 12.00 8.00 10.00 12.00 )][C Page 206 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S038 Description: 1,,15uL Date: 11-Jan-2013 Time: 22:57:42 13C2-PFOA FC3G 013S038 100- 7.05 2.47e5 952397 %- 13C8-PFOA FC3G 013S038 100- - 7.05 k 8.34e4 .323247 %- 13C4-PFOA FC3G 013S038 100- - 7.05 1.07e5 416902 %" 0 - - rn--t]*--------, 7.00 - p-r-rr-Tr mmmin 8.00 u0----- --; ' 7.00 i , ! | i-ir-r mmi nin 8.00 140u - , 7.00 |r 8.00 1802-PFHxS-2 FC3G 013S038 100- 7.29 f 7.23e3 - ' ' 28847 13C4-PFBA FC3G 013S038 100- 5.19 2.56e4 : 90175 13C2-PFHxA FC3G_013S038 100p 6.33 6.51e4 J : 299879 % - % - %: 7.00 8.00 . 4.13 _ 111" " `I" 1"7rTTMTrm|i|T min 5.00 6.00 7.00 5.00 6.00 7.00 13C8-PFOSA FC3G_013S038 100 9.99 1.44e4l 71975 %- 13C2-PFOUEA FC3G_013S038 tttjtt min 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S038 100- 8.29 T 1,06e4 1 40433 %- H 13C4-PFOS-2 FC3G 013S038 100- 8.29 i f 8.59e3 H 31283 %- ..`Jf-rirlr-rjj-i i 8.00 10.00 12^00 8.00 10.00 12^00 13C5-PFNA FC3G 013S038 100- 7.46 8.10c4 l 337008 ft % Mi , . \iM~wI I Ii^n-rr min 7.00 8.00 13C2-PFDA FC3G 013S038 100- 7.99 T 9.58e4 g 340457 I l H o: ` iI i i >1. 8.00 10.00 12.00 1802-PFHxS-1 FC3G 013S038 100- 7.29 JTl.07e4 40670 %- i i 7.00 8.00 13C2-PFDoA FC3G 013S038 100- 9.15 T 1.43e5 1 584559 %- :i him liffuWn i ii irrrni 8.00 10.00 12.00 m Page 207 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S038 Description: 1,,15uL Date: 11'Jan-2013 Time: 22:57:42 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.19 27 262.98 >218.... 5.95 27 312.97> 268.98 6.33 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.46 29 512.96 >468.... 7.99 29 562.96 >518.... 8.55 30 612.95 >568.... 9.15 24 298.94> 79.96 6.47 25 298.94 > 98.95 6.44 24 398.84 > 79.96 7.29 25 398.84> 98.96 7.29 24 398.84> 118.70 7.29 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.96 21 414.97 >369.... 7.05 21 420.97 > 375.... 7.05 416.97 >371.9 7.05 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.19 21 314.97>269.98 6.33 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.99 458.98 >393.98 7.33 Area 46783 69047 136739 137847 143677 35654 146649 196747 249575 243755 35074 18515 31945 17318 10159 28693 19846 26969 246572 83449 107314 10647 8588 10708 7226 25606 65085 81013 95783 143341 14423 35723 IS Area 25606 65085 65085 65085 246572 246572 81013 95783 95783 143341 10708 7226 10708 7226 10708 10647 8588 14423 107314 107314 107314 107314 107314 107314 107314 107314 107314 107314 107314 107314 ng/g 37.26' 35.274 40.904 36.798 38.805 42.592 37.764 40.032 42.117 45.507 86.254 86.292 81.072 82.148 78.688 75.597 77.346 41.334 72.037 12.452 13.851 31.043 30.716 31,818 32.285 23.307 24.785 22.969 20.316 18.422 19.803 12.635 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0255 0.0044 '0.0019 0.0131 0.0103 0.0499 0.0975 0.3189 0.3437 0.1200 0.2752 0.0483 0.0477 0.0603 0.0804 0.0774 0.0489 %Rec S/N 93.2 615 88.2 1598 102.3 2186 92.0 4171 97.0 1385 106.5 1639 94.4 5614 100.1 2423 105.3 3897 113.8 3291 107.8 3943 107.9 6867 101.3 4613 102.7 0 98.4 461 94.5 1836 96.7 749 103.3 2207 100.1 16363 103.8 3623 115.4 834 86.2 934 85.3 271 88.4 272 89.7 829 97.1 230 103.3 1826 95.7 1546 84.7 76.8 925 722 82.5 986 126.3 774 Noise 284 193 289 132 397 85 108 295 272 297 39 12 26 0 78 45 89 44 58 89 500 43 115 150 35 392 164 218 368 810 73 178 Height 174507 308459 631423 548581 549281 139673 608993 714684 1060816 976177 153073 84958 119600 62998 35856 82662 66270 97741 952397 323247 416902 40433 31283 40670 28847 90175 299879 337008 340457 584559 71975 137578 IS Height Sample size 90175 1.0000 299879 1.0000 299879 1.0000 299879 1.0000 952397 1.0000 952397 1.0000 337008 1.0000 340457 1.0000 340457 1.0000 584559 1.0000 40670 1.0000 28847 1.0000 40670 1.0000 28847 1.0000 40670 1.0000 40433 1.0000 31283 1.0000 71975 1.0000 416902 1.0000 416902 1.0000 1.0000 416902 1.0000 416902 1.0000 416902 1.0000 416902 1.0000 416902 1.0000 416902 1.0000 416902 1.0000 416902 1.0000 416902 1.0000 416902 1.0000 1.0000 Factor2 Detection.. 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 208 of 234 www.axysanalytical.com Matrix Codes for Table 1a N PW = Non-Potable W ater D rW = Drinking W ater S = Solid T = Tissue AXYS Analytical Services Ltd. Table 1a NELAP Accreditation Held by AXYS Analytical Services Ltd. for Chlorinated Dioxins/Furans, Chlorinated Pesticides, PCBs and PAHs Accreditation Method Codes and Explanation for Table 1a Code No. Accreditation Certificate Method Reference Applicable A X Y S Method and Description 1 EPA 1613B MLA-017, perform ance based im plem entation of EPA1613B (G C/HRM S) 2 EPA 8290 MLA-017, perform ance based im plem entation of EPA 8290 (G c /HRM S) 3 AXYS MLA-017 MLA-017, perform ance based im plem entation of EPA 1613B, 8290 (G C/HRM S) 4 EPA 608 MLA-007, perform ance based im plem entation of EPA 608 (G C/ECD) 5 EPA 8270C or 8270D M LA-007, perform ance based modification o f 8270C /D (G C /LR M S ) 6 E P A 8081Aor8081B MLA-007, perform ance based im plem entation of EPA 8 0 8 1 A/B (G C/ECD) 7 EPA 1668A MLA-010, perform ance based im plem entation of EPA 1668A (G C/HRM S) 8 SM 6630B MLA-007, perform ance based im plem entation of SM 18-20 6630B (GC/ECD) 9 EPA 1625B M LA-021, perform ance based modification o f E PA 1625B (G C /LR M S ) 11 E P A 625 M LA-007, perform ance based modification o f E P A 625 (g C /LR M S ) 20 EPA 8270C or 8270D M LA-021, perform ance based modification o f E P A 82 70 C /D (G C /LR M S ) 21 EPA 1625B M LA-007, perform ance based modification o f E PA 1625B (G C /LR M S ) ACC-101 Rev 08, 08-Nov-2012 Pa9e, o J l < * Page 209 of 234 www.axysanalytical.com TABLE 1a PCDD/F - Polychlorinated Dioxins and Furans D io x in s Dioxins and Dibenzofurans 1,2,3,4 ,6 ,7 ,8 -H p C D D 1,2,3,4 ,6 ,7 ,8 -H p C D F 1,2,3,4 ,7 ,8 ,9 -H p C D F 1,2 ,3 ,4 ,7 ,8 -H xC D D 1,2 ,3 ,4 ,7 ,8 -H xC D F 1,2 ,3 ,6 ,7 ,8 -H xC D D 1,2 ,3 ,6 ,7 ,8 -H xC D F 1,2 ,3 ,7 ,8 ,9 -H xC D D 1,2 ,3 ,7 ,8 ,9 -H xC D F 1 ,2 ,3 ,7 ,8 -P e C D D 1,2 ,3 ,7 ,8 -P e C D F 2,3 ,4 ,6 ,7 ,8 -H xC D F 2,3 ,4 ,7 ,8 -P e C D F 2 ,3 ,7 ,8 -T C D D 2 ,3 ,7 ,8 -T C D F OCDD OCDF Total TCDD Total TCDF Total PeCDD Total PeCDF Total HxCDD Total HxCDF Total HpCDD Total HpCDF PCBs - Polychlorinated biphenyls PCB 1 2-Chlorobiphenyl PCB 3 4-Chlorobiphenyl PCB 4 2,2'-D ichlorobiphenyl PCB 5 2,3-Dichlorobiphenyl PCB 15 4,4'-D ichlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. New York State D ep artm en t of Health Lab ID 11674 NELAP Primary NP S W C a lifo rn ia D ep artm en t of Public Health Lab ID 01138CA NELAP Secondary NP S W State of Florida D ep artm en t of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 1 2 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 1 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1 1, 2, 3 1, 2, 3 2, 3 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 State of New Jersey Departm ent of E n v iro n m e n ta l Protection Lab ID CANA005 NELAP Secondary Dr. NP WW S T 122 122 122 122 122 122 122 122 122 122 122 122 122 122 122 122 122 22 22 22 22 22 22 22 22 Virginia Division of Consolidated Laboratory Services Lab ID 460224 NELAP Secondary NP S W T 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 22 22 22 22 22 22 22 22 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 mPage 2 o f J? Page 210 of 234 www.axysanalytical.com TABLE 1a PCB 18 PCB 19 PCB 31 PCB 37 PCB 44 PCB 52 PCB 54 PCB 66 PCB 77 PCB 81 PCB 87 PCB 101 PCB 104 PCB 105 PCB 109 PCB 114 PCB 118 PCB 123 PCB 124 PCB 126 PCB 138 PCB 141 PCB 151 PCB 153 PCB 155 PCB 156 PCB 157 PCB 167 PCB 169 PCB 170 PCB 180 PCB 183 PCB 187 PCB 188 PCB 189 PCB 202 2,2',5-T richlorobiphenyl 2,2',6-T richlorobiphenyl 2 ,4 ',5 -T ric h lo ro b ip h e n y l 3 ,4 ,4 '-T ric h lo ro b ip h e n y l 2 ,2 ',3 ,5 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',5 ,5 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',6 ,6 '-T e tra c h lo ro b ip h e n y l 2 ,3 ',4 ,4 '-T e tra c h lo ro b ip h e n y l 3 ,3 ',4 ,4 '-T e tra c h lo ro b ip h e n y l 3 ,4 ,4 ',5 -T e tra c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',4 ,5 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',4 ,6 ,6 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ,4 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ,6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ,4 ',5 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ',5 ,5 '-P e n ta c h lo ro b ip h e n y l 3 ,3 ',4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2,2',3,4,4',5'-H exachlorobiphenyl 2,2',3,4,5,5'-H exachlorobiphenyl 2,2',3,5,5',6-H exachlorobiphenyl 2,2',4,4',5,5'-H exachlorobiphenyl 2,2',4,4',6,6'-H exachlorobiphenyl 2,3,3',4,4',5-H exachlorobiphenyl 2,3,3',4,4',5'-H exachlorobiphenyl 2,3',4,4',5,5'-H exachlorobiphenyl 3,3',4,4',5,5'-H exachlorobiphenyl 2,2',3,3',4,4',5-H eptachlorobiphenyl 2,2',3,4,4',5,5'-H eptachlorobiphenyl 2,2',3,4,4',5',6-H eptachlorobiphenyl 2,2',3,4',5,5',6-H eptachlorobiphenyl 2,2',3,4',5,6,6'-H eptachlorobiphenyl 2,3,3',4,4',5,5'-H eptachlorobiphenyl 2,2',3,3',5,5',6,6'-O ctachlorobiphenyl A C C-101 R ev 08, 08-Nov-2012 AXYS Analytical Services Ltd. New York State D ep artm en t of Health Lab ID 11674 NELAP Primary NP S W 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 C a lifo rn ia D ep artm en t of Public Health Lab ID 01138CA NELAP Secondary NP S W State of Florida D ep artm en t of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 77 77 77 77 77 77 7 77 77 77 77 77 77 77 77 77 77 State of New Jersey Departm ent of E n v iro n m e n ta l Protection Lab ID CANA005 NELAP Secondary Dr. NP WW 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 S 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 T Virginia Division of Consolidated Laboratory Services Lab ID 460224 NELAP Secondary NP S W 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 7 77 77 77 77 77 77 77 77 77 77 T Page 3 o f-J * Page 211 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 1a PCB 205 PCB 206 PCB 208 PCB 209 Aroclor 1260 Aroclor 1254 Aroclor 1221 Aroclor 1232 Aroclor 1248 Aroclor 1016 Aroclor 1242 2,3,3',4,4',5,5',6-Octachlorobiphenyl 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 2,2',3,3',4,5,5',6,6'-Nonachlorobiphenyl Decachlorobiphenyl Pesticides 4,4'-DDD 4,4'-DDE 4,4'-DDT Aldrin Alpha-HCH Beta-HCH cis-Chlordane (alpha-Chlordane) Chlordane, technical Delta-HCH Dieldrin Endosulphan I Endosulphan II Endosulphan sulphate Endrin Endrin aldehyde trans-Chlordane (gamma-Chlordane) Gamma-HCH (Lindane) Heptachlor Heptachlor epoxide Hexachlorobenzene Methoxychlor Mi rex New York State D ep artm en t of Health Lab ID 11674 NELAP Primary NP S W 7 7 7 7 7, 11 7, 11 7, 11 7, 11 7, 11 7, 11 7, 11 7 7 7 7 5, 7 5, 7 5, 7 5, 7 5, 7 5, 7 5, 7 C a lifo rn ia D ep artm en t of Public Health Lab ID 01138CA NELAP Secondary NP S W 11 5 11 5 11 5 11 5 11 5 11 5 11 5 State of Florida D ep artm en t of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 55 5, 11 5 11 5 11 5 11 5 4646 4646 4646 4646 4646 4646 55 11 5 11 5 11 5 11 5 4646 21 5 9 5 4, 8 6 8 6 5 ACC-101 Rev 08, 08-Nov-2012 State of New Jersey Departm ent of E n v iro n m e n ta l Protection Lab ID CANA005 NELAP Secondary Dr. NP WW 7 7 7 7 S 7 7 7 7 T Virginia Division of Consolidated Laboratory Services Lab ID 460224 NELAP Secondary NP S W 77 77 77 77 11 5 11 5 11 5 11 5 11 5 11 5 11 5 T 11 11 11 11 11 11 5, 11 11 4 4 4 4 4 4 11 11 4 4 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 5 5 6 5 6 Page 4 o f Page 212 of 234 www.axysanalytical.com TABLE 1a PAH A n th ra c e n e Pyrene B e n z o [g h ilp e ry le n e Indeno[1,2,3-cdlpyrene B e n z o [b lflu o ra n th e n e Fluoranthene B e n z o [k ]flu o ra n th e n e A c e n a p h th y le n e Chrysene Benzo[a]pyrene D ib e n z [a h la n th ra c e n e B e n z [a la n th ra c e n e A c e n a p h th e n e P h e n a n th re n e F lu o re n e N a p h th a le n e AXYS Analytical Services Ltd. New York State D ep artm en t of Health Lab ID 11674 NELAP Primary NP S W C a lifo rn ia D ep artm en t of Public Health Lab ID 01138CA NELAP Secondary NP S W State of Florida D ep artm en t of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 9 20 9 20 9 20 9 20 9 20 9 20 9 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 State of New Jersey Departm ent of E n v iro n m e n ta l Protection Lab ID CANA005 NELAP Secondary Dr. NP WW S T Virginia Division of Consolidated Laboratory Services Lab ID 460224 NELAP Secondary NP S W T 9 20 9 20 9 20 9 20 9 20 9 20 9 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 ACC-101 Rev 08, 08-Nov-2012 Pa9e5oJ l < * Page 213 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Table 1b NELAP Accreditation Held by AXYS Analytical Services Ltd. for Perfluorinated Organic Compounds Matrix Codes for Table 1b N PW = Non-Potable Water D rW = Drinking W ater S = Solid T = Tissue Accreditation Method Codes and Explanation for Table 1b Code Accreditation Certificate Applicable A X Y S Method and Description No. Method Reference 12 AXYS MLA-041 13 AXYS M LA-043 14 AXYS M LA-060 MLA-041, laboratory perform ance based method (LC /M S-M S) MLA-043, laboratory perform ance based method (LC /M S-M S) MLA-060, laboratory perform ance based method (LC /M S-M S) TA BLE 1b State of Florida D ep artm en t of Health Lab ID E871007 NELAP Primary Dr. NP S WW T Minnesota Department of Health Lab ID 232-999-430 NELAP Primary! Dr. NP S T WW State of New Jersey Departm ent of E n v iro n m e n ta l Protection Lab ID CANA005 NELAP Secondary Dr. NP S T WW PFC - Perfluorinated Organic Compounds P erflu oro buta noa te (P F B A ) Note P erflu o ro p e n ta n o a te (P F P eA ) Note P erflu oro hexa no ate (P F H xA ) Note P erflu o ro h e p ta n o a te (P F H pA ) Note Perfluorooctanoate (PFOA) Note P erflu o ro n o n a n o a te (P F N A ) Note P erflu oro deca no ate (P F D A ) Note P erflu o ro u n d e ca n o a te (P F U nA ) Note P erflu o ro d o d e ca n o a te (P F D oA ) Note Perfluorobutanesulfonate (PFBS) Perfluorohexanesulfonate (PFHxS) Perfluorooctanesulfonate (PFOS) Perfluorooctane sulfonam ide (PFOSA) 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 Note: A ccreditations by M innesota Departm ent of Health and New Jersey D epartm ent of Environm ental Protection are against the corresponding acid form of the anion shown. ACC-101 Rev 08, 08-Nov-2012 Pa9e6oJ l & Page 214 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Table 2: Canadian and US State Specific Accreditation Held by AXYS Analytical Services Ltd. Matrix Codes for Table 2 NP W = Non-Potable Water Dr. W = Drinking W ater W= Aqueous S = Solid T = Tissue Ser = Serum/Plasma U = Urine Accreditation Method Codes and Explanation for Table 2 Code No. Accreditation Certificate Method Reference Applicable A X Y S Method and Description 1 EPA 1613 3 AXYS MLA-017 7 EPA 1668A 10 A X Y S M LA-007 12 A X Y S MLA-041 13 A X Y S M LA-043 14 A X Y S M LA-060 15 A X Y S M LA-010 16 A X Y S M LA-028 17 A X Y S M LA-033 18 A X Y S MLA-021 19 A X Y S M LA-075 22 AXYS MLA-901 23 AXYS MLA-042 24 AXYS MLA-070 25 AXYS MLA-079 26 AXYS MLA-059 27 AXYS MLA-064 28 EPA 1668C 29 AXYS MLA-021 M LA-017 Perform ance based im plem entation of EPA 1613B (G C/HRM S) M LA -017 P erform ance based im plem entation o f E P A 1613B (gC/HRMs) M LA -010 P erform ance based im plem entation o f E P A 1668A (gC/HRMs) M LA-007, P erfo rm a nce based modification o f E P A 8270C /D , 8081 A /B (G C /LR M S and G C /E C D ) MLA-041 Laboratory perform ance based method (LC/M S-M S) M LA-043 Laboratory perform ance based method (LC/M S-M S) M LA-060 Laboratory perform ance based method (LC/M S-M S) M LA-010 Perform ance based im plem entation of EPA 1668A (G C/HRM S) M LA-028 Laboratory perform ance based method (GC/HRM S) M LA -033 P erform ance based im plem entation o f EP A 1614 (Gc/HRMS) M LA-021 P erfo rm a nce based modification o f E P A 82 70 C /D (G C /LR M S ) M LA-075 Perform ance based im plem entation of EPA 1694 (LC/M S-M S) M LA-901 Laboratory perform ance based m ethod (GC/LRmS) M LA-042 Laboratory perform ance based method (LC/M S-M S) M LA-070 Laboratory perform ance based method (LC/M S-M S) M LA-079 Laboratory perform ance based method (LC/M S-M S) M LA-059 Laboratory perform ance based method (LC/M S-M S) M LA-064 Laboratory perform ance based method (LC/M S-M S) M LA-010 Perform ance based im plem entation of EPA 1668C (G C/HRM S) M LA-021 P erfo rm a nce based modification o f E P A 1625 (G C /LR M S ) ACC-101 Rev 08, 08-Nov-2012 Pa9e 7 o J l < * Page 215 of 234 www.axysanalytical.com TABLE 2 PCDD/F - Polychlorinated Dioxins and Furans 1 ,2 ,3 ,4 ,6 ,7 ,8 -H p C D D 1 ,2 ,3 ,4 ,6 ,7 ,8 -H p C D F 1 ,2 ,3 ,4 ,7 ,8 ,9 -H p C D F 1,2 ,3 ,4 ,7 ,8 -H x C D D 1,2 ,3 ,4 ,7 ,8 -H x C D F 1,2 ,3 ,6 ,7 ,8 -H x C D D 1,2 ,3 ,6 ,7 ,8 -H x C D F 1,2 ,3 ,7 ,8 ,9 -H x C D D 1,2 ,3 ,7 ,8 ,9 -H x C D F 1 ,2 ,3 ,7 ,8 -P e C D D 1,2 ,3 ,7 ,8 -P e C D F 2,3 ,4 ,6 ,7 ,8 -H xC D F 2,3 ,4 ,7 ,8 -P e C D F 2 ,3 ,7 ,8 -T C D D 2 ,3 ,7 ,8 -T C D F OCDD OCDF PCBs - Polychlorinated biphenyls PCB 1 2-Chlorobiphenyl PCB 2 3-Chlorobiphenyl PCB 3 4-Chlorobiphenyl PCB 4 2,2'-D ichlorobiphenyl PCB 5 2,3-Dichlorobiphenyl PCB 6 2,3'-D ichlorobiphenyl PCB 7 2,4-Dichlorobiphenyl PCB 8 2,4'-D ichlorobiphenyl PCB 8/5 PCB 9 2,5-Dichlorobiphenyl PCB 10 2,6-Dichlorobiphenyl PCB 11 3,3'-D ichlorobiphenyl PCB 12 3,4-Dichlorobiphenyl PCB 13 3,4'-D ichlorobiphenyl PCB 14 3,5-Dichlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 10 10 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 Pa9eSoJ l & Page 216 of 234 www.axysanalytical.com TABLE 2 PCB 15 4,4'-D ichlorobiphenyl PCB 16 2,2',3-T richlorobiphenyl PCB 16/32 PCB 17 2,2',4-T richlorobiphenyl PCB 18 2 ,2 ',5 -T ric h lo ro b ip h e n y l PCB 19 2,2',6-T richlorobiphenyl PCB 20 2 ,3 ,3 '-T ric h lo ro b ip h e n y l PCB 21 2,3,4-T richlorobiphenyl PCB 22 2 ,3 ,4 '-T ric h lo ro b ip h e n y l PCB 23 2,3,5-T richlorobiphenyl PCB 24 2,3,6-T richlorobiphenyl PCB 24/27 PCB 25 2,3',4-T richlorobiphenyl PCB 26 2 ,3 ',5 -T ric h lo ro b ip h e n y l PCB 27 2 ,3 ',6 -T ric h lo ro b ip h e n y l PCB 28 2 ,4 ,4 '-T ric h lo ro b ip h e n y l PCB 29 2,4,5-T richlorobiphenyl PCB 30 2,4,6-T richlorobiphenyl PCB 31 2 ,4 ',5 -T ric h lo ro b ip h e n y l PCB 32 2 ,4 ',6 -T ric h lo ro b ip h e n y l PCB 33 2 ,3 ',4 '-T ric h lo ro b ip h e n y l PCB 33/20/21 PCB 34 2 ,3 ',5 '-T ric h lo ro b ip h e n y l PCB 35 3 ,3 ',4 -T ric h lo ro b ip h e n y l PCB 36 3 ,3 ',5 -T ric h lo ro b ip h e n y l PCB 37 3 ,4 ,4 '-T ric h lo ro b ip h e n y l PCB 38 3,4,5-T richlorobiphenyl PCB 39 3 ,4 ',5 -T ric h lo ro b ip h e n y l PCB 40 2 ,2 ',3 ,3 '-T e tra c h lo ro b ip h e n y l PCB 41 2 ,2 ',3 ,4 -T e tra c h lo ro b ip h e n y l PCB 41/71/64/68 PCB 42 2 ,2 ',3 ,4 '-T e tra c h lo ro b ip h e n y l PCB 42/59 PCB 43 2 ,2 ',3 ,5 -T e tra c h lo ro b ip h e n y l PCB 44 2 ,2 ',3 ,5 '-T e tra c h lo ro b ip h e n y l ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Accreditation no.: A 2637 W S Pulp T Ser 10, 15 15 10 10, 15 10, 15 10, 15 15 15 10, 15 15 15 10 10, 15 10, 15 15 10, 15 15 15 10, 15 15 15 18 15 15 15 15 15 15 10, 15 15 10 15 10 15 10, 15 10, 15 15 10 10, 15 10, 15 10, 15 15 15 10, 15 15 15 10 10, 15 10, 15 15 10, 15 15 15 10, 15 15 15 10 15 15 15 15 15 15 10, 15 15 10 15 10 15 10, 15 10, 15 15 10 10, 15 10, 15 10, 15 15 15 10, 15 15 15 10 10, 15 10, 15 15 10, 15 15 15 10, 15 15 15 10 15 15 15 15 15 15 10, 15 15 10 15 10 15 10, 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 W ashing to n State D epartm ent of Ecology Lab. ID: C404 U NP S W 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 Pa9e9oJ l < * Page 217 of 234 www.axysanalytical.com TABLE 2 PCB 45 PCB 46 PCB 47 PCB 47/48/75 PCB 48 PCB 49 PCB 49/43 PCB 50 PCB 51 PCB 52 PCB 52/73 PCB 53 PCB 54 PCB 55 PCB 56 PCB 56/60 PCB 57 PCB 58 PCB 59 PCB 60 PCB 61 PCB 62 PCB 63 PCB 64 PCB 65 PCB 66 PCB 66/80 PCB 67 PCB 68 PCB 69 PCB 70 PCB 70/76 PCB 71 PCB 72 PCB 73 2 ,2 ',3 ,6 -T e tra c h lo ro b ip h e n y l 2 ,2 ',3 ,6 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',4 ,4 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',4 ,5 -T e tra c h lo ro b ip h e n y l 2 ,2 ',4 ,5 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',4 ,6 -T e tra c h lo ro b ip h e n y l 2 ,2 ',4 ,6 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',5 ,5 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',5 ,6 '-T e tra c h lo ro b ip h e n y l 2 ,2 ',6 ,6 '-T e tra c h lo ro b ip h e n y l 2 ,3 ,3 ',4 -T e tra c h lo ro b ip h e n y l 2 ,3 ,3 ',4 '-T e tra c h lo ro b ip h e n y l 2 ,3 ,3 ',5 -T e tra c h lo ro b ip h e n y l 2 ,3 ,3 ',5 '-T e tra c h lo ro b ip h e n y l 2 ,3 ,3 ',6 -T e tra c h lo ro b ip h e n y l 2 ,3 ,4 ,4 -T e tra c h lo ro b ip h e n y l 2 ,3 ,4 ,5 -T e tra c h lo ro b ip h e n y l 2 ,3 ,4 ,6 -T e tra c h lo ro b ip h e n y l 2 ,3 ,4 ',5 -T e tra c h lo ro b ip h e n y l 2 ,3 ,4 ',6 -T e tra c h lo ro b ip h e n y l 2 ,3 ,5 ,6 -T e tra c h lo ro b ip h e n y l 2 ,3 ',4 ,4 '-T e tra c h lo ro b ip h e n y l 2 ,3 ',4 ,5 -T e tra c h lo ro b ip h e n y l 2 ,3 ',4 ,5 '-T e tra c h lo ro b ip h e n y l 2 ,3 ',4 ,6 -T e tra c h lo ro b ip h e n y l 2 ,3 ',4 ',5 -T e tra c h lo ro b ip h e n y l 2 ,3 ',4 ',6 -T e tra c h lo ro b ip h e n y l 2 ,3 ',5 ,5 '-T e tra c h lo ro b ip h e n y l 2 ,3 ',5 ',6 -T e tra c h lo ro b ip h e n y l ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Accreditation no.: A 2637 W S Pulp T Ser 10, 15 10, 15 15 10 15 15 10 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 10 15 15 15 10, 15 10, 15 15 10 15 15 10 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 10 15 15 15 10, 15 10, 15 15 10 15 15 10 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 W ashing to n State D epartm ent of Ecology Lab. ID: C404 U NP S W 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 Page 218 of 234 www.axysanalytical.com TABLE 2 PCB 74 PCB 74/61 PCB 75 PCB 76 PCB 77 PCB 78 PCB 79 PCB 80 PCB 81 PCB 82 PCB 83 PCB 83/108 PCB 84 PCB 85 PCB 85/120 PCB 86 PCB 87 PCB 87/115/116 PCB 88 PCB 89 PCB 90 PCB 91 PCB 92 PCB 93 PCB 94 PCB 95 PCB 95/93 PCB 96 PCB 97 PCB 97/86 PCB 98 PCB 99 PCB 100 PCB 101 PCB 101/90/89 2 ,4 ,4 ',5 -T e tra c h lo ro b ip h e n y l 2 ,4 ,4 ',6 -T e tra c h lo ro b ip h e n y l 2 ,3 ',4 ',5 '-T e tra c h lo ro b ip h e n y l 3 ,3 ',4 ,4 '-T e tra c h lo ro b ip h e n y l 3 ,3 ',4 ,5 -T e tra c h lo ro b ip h e n y l 3 ,3 ',4 ,5 '-T e tra c h lo ro b ip h e n y l 3 ,3 ',5 ,5 '-T e tra c h lo ro b ip h e n y l 3 ,4 ,4 ',5 -T e tra c h lo ro b ip h e n y l 2 ,2 ',3 ,3 ',4 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,3 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,3 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ,4 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ,5 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ,6 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ,6 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,5 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,5 ,6 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,5 ,6 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,5 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,6 ,6 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ',5 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',3 ,4 ',6 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',4 ,4 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',4 ,5 ,5 '-P e n ta c h lo ro b ip h e n y l ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 15 15 10 10 15 15, 22 10 28 28 15 15 15 15 28 28 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10, 15 10, 15 10 10, 15 15 28 28 15 15 15 15 28 28 10 10 15 15 10 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 15 15 10 15 15 28 28 15 15 15 15 28 28 10 10 15 15 10 15 15 28 28 10, 15 10, 15 10, 15 15, 22 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 Page 219 of 234 www.axysanalytical.com TABLE 2 PCB 102 PCB 103 PCB 104 PCB 105 PCB 105/127 PCB 106 PCB 107 PCB 107/109 PCB 108 PCB 109 PCB 110 PCB 111 PCB 112 PCB 113 PCB 114 PCB 115 PCB 116 PCB 117 PCB 118 PCB 118/116 PCB 119 PCB 120 PCB 121 PCB 122 PCB 123 PCB 124 PCB 125 PCB 126 PCB 127 PCB 128 PCB 129 PCB 130 PCB 131 PCB 131/142 PCB 132 2 ,2 ',4 ,5 ,6 '-P e n ta c h lo ro b ip h e n y l 2 ,2 ',4 ,5 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,2 ',4 ,6 ,6 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ,4 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ,5 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ,6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',5 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',5 ,6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',5 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,4 ,4 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,4 ,5 ,6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,4 ',5 ,6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ,4 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ,5 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ,5 ',6 -P e n ta c h lo ro b ip h e n y l 2 ,3 ,3 ',4 ',5 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ,4 ',5 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ',5 ,5 '-P e n ta c h lo ro b ip h e n y l 2 ,3 ',4 ',5 ',6 -P e n ta c h lo ro b ip h e n y l 3 ,3 ',4 ,4 ',5 -P e n ta c h lo ro b ip h e n y l 3 ,3 ',4 ,5 ,5 '-P e n ta c h lo ro b ip h e n y l 2,2',3,3',4,4'-H exachlorobiphenyl 2,2',3,3',4,5-H exachlorobiphenyl 2,2',3,3',4,5'-H exachlorobiphenyl 2,2',3,3',4,6-H exachlorobiphenyl 2,2',3,3',4,6'-H exachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 15 15 15 15 15 15 15 15 28 28 28 28 15 15 15 15 28 28 15 15 10 10 15 15 10 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 15 15 15 15 28 28 28 28 15 15 15 15 28 28 10, 15 10, 15 15 15 10, 15 15 15 15 28 28 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15, 22 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 15 15 28 28 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 Page 220 of 234 www.axysanalytical.com TABLE 2 PCB 133 2,2',3,3',5,5'-H exachlorobiphenyl PCB 134 2,2',3,3',5,6-H exachlorobiphenyl PCB 134/143 PCB 135 2,2',3,3',5,6'-H exachlorobiphenyl PCB 136 2,2',3,3',6,6'-H exachlorobiphenyl PCB 137 2,2',3,4,4',5-H exachlorobiphenyl PCB 138 2,2',3,4,4',5'-H exachlorobiphenyl PCB 138/163/164 PCB 139 2,2',3,4,4',6-H exachlorobiphenyl PCB 140 2,2',3,4,4',6'-H exachlorobiphenyl PCB 141 2,2',3,4,5,5'-H exachlorobiphenyl PCB 142 2,2',3,4,5,6-H exachlorobiphenyl PCB 143 2,2',3,4,5,6'-H exachlorobiphenyl PCB 144 2,2',3,4,5',6-H exachlorobiphenyl PCB 144/135 PCB 145 2,2',3,4,6,6'-H exachlorobiphenyl PCB 146 2,2',3,4',5,5'-H exachlorobiphenyl PCB 147 2,2',3,4',5,6-H exachlorobiphenyl PCB 148 2,2',3,4',5,6'-H exachlorobiphenyl PCB 149 2,2',3,4',5',6-H exachlorobiphenyl PCB 149/139 PCB 150 2,2',3,4',6,6'-H exachlorobiphenyl PCB 151 2,2',3,5,5',6-H exachlorobiphenyl PCB 152 2,2',3,5,6,6'-H exachlorobiphenyl PCB 153 2,2',4,4',5,5'-H exachlorobiphenyl PCB 154 2,2',4,4',5,6'-H exachlorobiphenyl PCB 155 2,2',4,4',6,6'-H exachlorobiphenyl PCB 156 2,3,3',4,4',5-H exachlorobiphenyl PCB 157 2,3,3',4,4',5'-H exachlorobiphenyl PCB 158 2,3,3',4,4',6-H exachlorobiphenyl PCB 158/160 PCB 159 2,3,3',4,5,5'-H exachlorobiphenyl PCB 160 2,3,3',4,5,6-H exachlorobiphenyl PCB 161 2,3,3',4,5',6-H exachlorobiphenyl PCB 162 2,3,3',4',5,5'-H exachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 15 15 15 15 15 15 15 15 28 28 28 28 10 10 10 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15, 22 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 15 15 15 15 28 28 28 28 15 15 15 15 28 28 10 10 15 15 10 15 15 28 28 10, 15 10, 15 10, 15 15, 22 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15, 22 28 28 15 15 15 15 28 28 15 15 10, 15 10, 15 15 15 10, 15 15, 22 28 28 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 10 10 15 15 10 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 Page 221 of 234 www.axysanalytical.com TABLE 2 PCB 163 PCB 164 PCB 165 PCB 166 PCB 167 PCB 168 PCB 169 PCB 170 PCB 170/190 PCB 171 PCB 172 PCB 172/192 PCB 173 PCB 174 PCB 174/181 PCB 175 PCB 176 PCB 177 PCB 178 PCB 179 PCB 180 PCB 181 PCB 182 PCB 183 PCB 184 PCB 185 PCB 186 PCB 187 PCB 187/182 PCB 188 PCB 189 PCB 190 PCB 191 PCB 192 PCB 193 2,3,3',4',5,6-H exachlorobiphenyl 2,3,3',4',5',6-H exachlorobiphenyl 2,3,3',5,5',6-H exachlorobiphenyl 2,3,4,4',5,6-H exachlorobiphenyl 2,3',4,4',5,5'-H exachlorobiphenyl 2,3',4,4',5',6-H exachlorobiphenyl 3,3',4,4',5,5'-H exachlorobiphenyl 2,2',3,3',4,4',5-H eptachlorobiphenyl 2,2',3,3',4,4',6-H eptachlorobiphenyl 2,2',3,3',4,5,5'-H eptachlorobiphenyl 2,2',3,3',4,5,6-H eptachlorobiphenyl 2,2',3,3',4,5,6'-H eptachlorobiphenyl 2,2',3,3',4,5',6-H eptachlorobiphenyl 2,2',3,3',4,6,6'-H eptachlorobiphenyl 2,2',3,3',4,5',6'-H eptachlorobiphenyl 2,2',3,3',5,5',6-H eptachlorobiphenyl 2,2',3,3',5,6,6'-H eptachlorobiphenyl 2,2',3,4,4',5,5'-H eptachlorobiphenyl 2,2',3,4,4',5,6-H eptachlorobiphenyl 2,2',3,4,4',5,6'-H eptachlorobiphenyl 2,2',3,4,4',5',6-H eptachlorobiphenyl 2,2',3,4,4',6,6'-H eptachlorobiphenyl 2,2',3,4,5,5',6-H eptachlorobiphenyl 2,2',3,4,5,6,6'-H eptachlorobiphenyl 2,2',3,4',5,5',6-H eptachlorobiphenyl 2,2',3,4',5,6,6'-H eptachlorobiphenyl 2,3,3',4,4',5,5'-H eptachlorobiphenyl 2,3,3',4,4',5,6-H eptachlorobiphenyl 2,3,3',4,4',5',6-H eptachlorobiphenyl 2,3,3',4,5,5',6-H eptachlorobiphenyl 2,3,3',4',5,5',6-H eptachlorobiphenyl AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 15 15 15 15 15 15 15 15 28 28 28 28 15 15 15 15 28 28 15 15 15 15 15 15 15 15 28 28 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15, 22 28 28 10 10 10 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10 10 15 15 10 15 15 28 28 15 15 15 15 28 28 10 10 10, 15 10, 15 10 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15, 22 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 15 15 15 15, 22 28 28 28 28 10 10 10 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 ACC-101 Rev 08, 08-Nov-2012 Page 222 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 Canadian Association for Laboratory Accreditation (CALA) Accreditation no.: A 2637 W ashing to n State D epartm ent of Ecology Lab. ID: C404 W S Pulp T Ser U NP S W PCB 194 PCB 195 2,2',3,3',4,4',5,5'-O ctachlorobiphenyl 2,2',3,3',4,4',5,6-O ctachlorobiphenyl 10, 15 10, 15 10, 15 10, 15 10, 15 15, 22 10, 15 15 28 28 28 28 PCB 196 2,2',3,3',4,4',5,6-O ctachlorobiphenyl 15 15 15 15 28 28 PCB 196/203 PCB 197 2,2',3,3',4,4',6,6'-O ctachlorobiphenyl 10 10 10, 15 10, 15 10 10, 15 15 28 28 PCB 198 2,2',3,3',4,5,5',6-O ctachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 199 2,2',3,3',4,5,5',6'-O ctachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 200 2,2',3,3',4,5,6,6'-O ctachlorobiphenyl 15 15 15 15 28 28 PCB 201 2,2',3,3',4,5',6,6'-O ctachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 202 2,2',3,3',5,5',6,6'-O ctachlorobiphenyl 15 15 15 15 28 28 PCB 203 2,2',3,4,4',5,5',6-O ctachlorobiphenyl 15 15 15 15 28 28 PCB 204 PCB 205 2,2',3,4,4',5,6,6'-O ctachlorobiphenyl 2,3,3',4,4',5,5',6-O ctachlorobiphenyl 15 15 10, 15 10, 15 15 10, 15 15 15 28 28 28 28 PCB 206 2,2',3,3',4,4',5,5',6-N onachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 207 PCB 208 2,2',3,3',4,4',5,6,6'-N onachlorobiphenyl 2,2',3,3',4,5,5',6,6'-N onachlorobiphenyl 10, 15 10, 15 10, 15 10, 15 10, 15 10, 15 15 15 28 28 28 28 PCB 209 D e c a c h lo ro b ip h e n y l 10, 15 10, 15 10, 15 15 28 28 Total M onochlorobiphenyls 15 15 15 15 28 28 Total Dichlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Trichlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Tetrachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Pentachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Hexachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Heptachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total O ctachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Nonachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Decachlorobiphenyls Total Polychlorinated biphenyls 10 10 10 10 10 10 Aroclors Aroclor 1260 Aroclor 1254 Aroclor 1268 A roclor 1221 Aroclor 1232 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 ACC-101 Rev 08, 08-Nov-2012 Page 223 of 234 www.axysanalytical.com TABLE 2 Aroclor 1248 Aroclor 1016 Aroclor 1242 Aroclor 1242/1016 Pesticides 2,4'-DD D 2,4'-DD E 2,4'-D D T 4,4'-DD D 4,4'-DD E 4,4'-D D T A ld rin A lp h a -H C H Beta-HCH cis-C hlordane (alpha-Chlordane) cis-Nonachlor D e lta -H C H Dieldrin Endosulphan I E ndosu lph an II Endosulphan sulphate Endrin Endrin aldehyde Endrin ketone G am m a-HCH (Lindane) H eptachlor H eptachlor epoxide H exachlorobenzene H exachlorobutadiene M ethoxychlor M ire x O x y c h lo rd a n e Toxaphene trans-C hlordane (gam m a-Chlordane) ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 10 10 10 10 10 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 Page 224 of 234 www.axysanalytical.com TABLE 2 trans-N onachlor BDE - Brominated Diphenylethers BDE 7 2,4-dibrom odiphenylether BDE 8 2,4'-dibrom odiphenylether BDE 10 2,6-dibrom odiphenylether BDE 11 3,3'-dibrom odiphenylether BDE 12 3,4-dibrom odiphenylether BDE 13 3,4'-dibrom odiphenylether BDE 15 4,4'-dibrom odiphenylether BDE 17 2,2',4-tribrom odiphenylether BDE 25 2,3',4-tribrom odiphenylether BDE 28 2,4,4'-tribrom odiphenylether BDE 30 2,4,6-tribrom odiphenylether BDE-33 2',3,4-tribrom odiphenylether BDE 35 3,3',4-tribrom odiphenylether BDE 37 3,4,4'-tribrom odiphenylether BDE 47 2,2',4,4'-tetrabrom odiphenylether BDE 49 2,2',4,5'-tetrabrom odiphenylether BDE 66 2,3',4,4'-tetrabrom odiphenylether BDE 75 2,4,4',6-tetrabrom odiphenylether BDE 77 3,3',4,4'-tetrabrom odiphenylether BDE 85 2,2',3,4,4'-pentabrom odiphenylether BDE 99 2,2',4,4',5-pentabrom odiphenylether BDE 100 2,2',4,4',6-pentabrom odiphenylether BDE 105 2,3,3',4,4'-pentabrom odiphenylether BDE 116 2,3,4,5,6-pentabrom odiphenylether BDE 119 2,3',4,4',6-pentabrom odiphenylether BDE 126 3,3',4,4',5-pentabrom odiphenylether BDE 140 2,2',3,4,4',6'-hexabrom odiphenylether BDE 153 2,2',4,4',5,5'-hexabrom odiphenylether BDE 154 2,2',4,4',5',6-hexabrom odiphenylether BDE 155 2,2',4,4',6,6'-hexabrom odiphenylether BDE 166 2,3,4,4',5,6-hexabrom odiphenylether BDE 181 2,2',3,4,4',5,6-heptabrom odiphenylether ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U 16 10, 16 10, 16 10, 16 NP W 16 S 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 Page 225 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 BDE-183 BDE 190 BDE 206 BDE 207 BDE 208 BDE 209 2,2',3,4,4',5',6-heptabrom odiphenylether 2,3,3',4,4',5,6-heptabrom odiphenylether 2,2',3,3',4,4',5,5',6-nonabrom odiphenylether 2,2',3,3',4,4',5,6,6'-nonabrom odiphenylether 2,2',3,3',4,5,5',6,6'-nonabrom odiphenylether Decabrom odiphenylether PFC - Perfluorinated Organic Compounds Perfluorobutanoate (PFBA) Perfluoropentanoate (PFPeA) Perfluorohexanoate (PFHxA) Perfluoroheptanoate (PFHpA) Perfluorooctanoate (PFOA) Perfluorononanoate (PFNA) Perfluorodecanoate (PFDA) Perfluoroundecanoate (PFUnA) Perfluorododecanoate (PFDoA) Perfluorobutanesulfonate (PFBS) Perfluorohexanesulfonate (PFHxS) Perfluorooctanesulfonate (PFOS) Perfluorooctane sulfonam ide (PFOSA) PAH A n th ra c e n e Pyrene B e n z o [g h i]p e ry le n e Benzo[e]pyrene Indeno[1,2,3-cd]pyrene P e ry le n e B e n z o [b ]flu o ra n th e n e Fluoranthene B e n z o [j/k ]flu o ra n th e n e s B e n z o [k ]flu o ra n th e n e A c e n a p h th y le n e Chrysene W 17 17 17 17 17 17 14 14 14 14 14 14 14 14 14 14 14 14 14 29 29 29 29 29 29 29 29 29 29 29 29 Canadian Association for Laboratory Accreditation (CALA) Accreditation no.: A 2637 S Pulp T Ser 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 29 18 29 18 29 18 29 18 29 18 29 18 29 18 29 18 18 29 18 29 18 W ashing to n State D epartm ent of Ecology Lab. ID: C404 U NP W 17 17 17 17 17 17 S ACC-101 Rev 08, 08-Nov-2012 Page 226 of 234 www.axysanalytical.com TABLE 2 Benzo[a]pyrene D ib e n z [a h ]a n th ra c e n e B e n z [a ]a n th ra c e n e A ce n a p h th e n e P h e n a n th re n e F lu o re n e N a p h th a le n e 1-M ethylchrysene 1-M ethylnaphthalene 1-M ethylphenanthrene 1,2-Dim ethylnaphthalene 1,2,6-T rim ethylphenanthrene 1,4,6,7-Tetram ethylnaphthalene 1,7-Dim ethylfluorene 1,7-Dim ethylphenanthrene 1,8-Dim ethylphenanthrene 2-M ethylanthracene 2-M ethylfluorene 2-M ethylnaphthalene 2-M ethylphenanthrene 2/3-M ethyldibenzothiophenes 2,3,5-T rim ethylnaphthalene 2,3,6-T rim ethylnaphthalene 2,4-D im ethyldibenzothiophene 2,6-D im ethylnaphthalene 2,6-D im ethylphenanthrene 3-M ethylfluoranthene/ Benzo(a)fluorene 3-M ethylphenanthrene 3,6-D im ethylphenanthrene 5/6-M ethylchrysenes 5,9-D im ethylchrysene 7-M ethylbenzo(a)pyrene 9/4-M ethylphenanthrenes Biphenyl C 1 -A ce n a p h th e n e s ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 29 29 29 29 18 18 29 29 18 29 29 29 29 18 18 29 29 18 29 29 18 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 Page 227 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 C 1 -B e n z (a )a n th ra c e n e s /c h ry s e n e s C 1-Benzofluoranthenes/ Benzopyrenes C 1 -B ip h e n y ls C 1-Dibenzothiophene C 1 -F lu o ra n th e n e s /P y re n e s C 1 -F lu o re n e s C 1-Naphthalenes C 1 -P h e n a n th re n e s /A n th ra c e n e s C 2 -B e n z(a )a n th ra ce n e s/C h ryse n e s C 2-Benzofluoranthenes/ Benzopyrenes C 2 -B ip h e n y ls C 2-Dibenzothiophene C 2 -F lu o ra n th e n e s /P y re n e s C 2 -F lu o re n e s C 2-Naphthalenes C 2 -P h e n a n th re n e s /A n th ra c e n e s C 3 -B e n z(a )a n th ra ce n e s/C h ryse n e s C 3-Dibenzothiophene C 3 -F lu o ra n th e n e s /P y re n e s C 3 -F lu o re n e s C 3-Naphthalenes C 3 -P h e n a n th re n e s /A n th ra c e n e s C 4 -B e n z(a )a n th ra ce n e s/C h ryse n e s C 4-Dibenzothiophene C 4 -F lu o ra n th e n e s /P y re n e s C 4-Naphthalenes C 4 -P h e n a n th re n e s /A n th ra c e n e s D ib e n z o th io p h e n e Retene PPCP (Pharmaceutical and Personal Care Products) Acetam inophen Azithrom ycin C a ffe in e C a rb a d o x Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 19 19 19 19 19 19 19 19 ACC-101 Rev 08, 08-Nov-2012 Page 228 of 234 www.axysanalytical.com TABLE 2 C arbam azepine Cefotaxim e C ip ro flo x a c in Clarithrom ycin C lin a flo x a c in C lo x a c illin D e h y d ro n ife d ip in e D ig o x ig e n in Digoxin D iltia z e m 1,7-Dim ethylxanthine Diphenhydram ine E n ro flo x a c in E rythrom ycin Flum equine F lu o x e tin e Lincom ycin Lom efloxacin M iconazole N o rflo x a c in N o rge stim a te O floxacin Orm etoprim O xacillin O xolinic acid Penicillin G Penicillin V R o x ith ro m y c in Sarafloxacin S u lfa c h lo ro p y rid a z in e S u lfa d ia z in e Sulfadim ethoxine Sulfam erazine Sulfam ethazine Sulfam ethizole ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 Page 229 of 234 www.axysanalytical.com TABLE 2 Sulfam ethoxazole Sulfanilam ide Sulfathiazole T h ia b e n d a z o le Trim ethoprim T y lo s in Virginiam ycin Anhydrochlortetracycline (ACTC) A nhydrotetracycline (ATC) Chlortetracycline (CTC) Dem eclocycline D o x y c y c lin e 4-Epianhydrochlortetracycline (EACTC) 4-Epianhydrotetracycline (EATC) 4-Epichlortetracycline (ECTC) 4-Epioxytetracycline (EOTC) 4-Epitetracycline (ETC) Isochlortetracycline (ICTC) M inocycline O xytetracycline (OTC) Tetracycline (TC) Bisphenol A Furosem ide Gem fibrozil G lip iz id e G lyburide H y d ro c h lo ro th ia z id e 2 -h y d ro x y -ib u p ro fe n Ibuprofen Naproxen T ric lo c a rb a n Triclosan W arfarin ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 Page 230 of 234 www.axysanalytical.com TABLE 2 A lb u te ro l Am phetam ine A te n o lo l A to rv a s ta tin Cim etidine C lo n id in e C o d e in e C o tin in e Enalapril Hydrocodone M e tfo rm in Oxycodone R a n itid in e Triam terene A lp ra z o la m Am itriptyline Am lodipine B e n z o y le c g o n in e Benztropine Betam ethasone C o c a in e DEET (N,N-diethyl-m -toluam ide) Desm ethyldiltiazem Diazepam F lu o c in o n id e Fluticasone propionate H y d ro c o rtis o n e 10-hydroxy-am itriptyline M ep rob am ate M ethylprednisolone M etoprolol N o rflu o x e tin e N o rverap am il ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 Page 231 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 Paroxetine Prednisolone Prednisone Promethazine Propoxyphene Propranolol Sertraline Simvastatin Theophylline Trenbolone Trenbolone acetate Valsartan Verapamil HBCDD alpha-hexabromocyclododecane (a-HBCDD) beta-hexabromocyclododecane (b-HBCDD) gamma-hexabromocyclododecane (g-HBCDD) TBBPA Tetrabromobisphenol A BPA and MPE 4,4'-dihydroxy-2,2-diphenylpropane (Bisphenol A) (BPA) Mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) Mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP) Mono-(3-carboxypropyl) phthalate (MCPP) Mono-2-ethylhexyl phthalate (MEHP) Mono-benzyl phthalate (MBzP) Mono-butyl phthalate (MBP) (n + iso) Mono-cyclohexyl phthalate (MCHP) Mono-ethyl phthalate (MEP) Mono-iso-nonyl phthalate (MiNP) Mono-methyl phthalate (MMP) Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 S 24 24 24 25 26 26 26 26 26 26 26 26 26 26 26 ACC-101 Rev 08, 08-Nov-2012 Page 232 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 Canadian Association for Laboratory Accreditation (CALA) W ashing to n State D epartm ent of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W Parabens Benzylparaben (Benzyl 4-hydroxybenzoate) Butylparaben (n-Butyl 4-hydroxybenzoate and isobutyl 4hydroxybenzoate) Ethylparaben (Ethyl 4-hydroxybenzoate) Isopropylparaben (Isopropyl 4-hydroxybenzoate) Methylparaben (Methyl 4-hydroxybenzoate) n-Propylparaben (Propyl 4-hydroxybenzoate) 27 27 27 27 27 27 Table 1 and Table 2 - Explanation of Terms Used: NELAP = National Environmental Laboratory Accreditation Program Non-potable water = water not fit for consumption without treatment as it may contain pollutants, contaminants, minerals or infective agents. Surface water, ground water, rainwater, effluents as well as any other non-drinking water sources are included in this category. Solid = environmental solid sample. Soil, sediment, biosolids, hazardous waste, mixed phase samples with significant solids content are included in this category. Performance based implementation = methodology follows that of the method reference but modifications deemed by AXYS as minor 1may apply, results meet method reference data quality standard. Performance based modification = modifications deemed by AXYS as significant2 have been made to method reference protocol, results meet method reference accuracy standard. The suitability of the methodology for any method prescriptive applications should be assessed based on the modifications made and the specific work requirements. Performance based method = an in-house AXYS method, published method reference not applicable. GC/LRMS = gas chromatography, low resolution mass spectrometry detection. GC/HRMS = gas chromatography, high resolution mass spectrometry detection. GC/ECD = gas chromatography, electron capture detection. LC/MS-MS = liquid chromatography, mass spectrometry-mass spectrometry detection. ACC-101 Rev 08, 08-Nov-2012 Page 233 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Note 1: Performance Based Implementation - Examples of Minor Modifications - use of additional isotopically labeled references - adjustment of calibration range - adjustment of clean-up technique - use of a different extraction of same general type (example soxhlet vs soxhlet Dean Stark) - addition of matrix type using same principles (example addition of tissue matrix using same detection principle and similar extraction type) Note 2: Performance Based Modification - Examples of Significant Modifications - different acquisition conditions using same detection principle (example MS SIM vs. full scan) - different internal control limits while meeting method reference accuracy standard ACC-101 Rev 08, 08-Nov-2012 Page 234 of 234 APPENDIX E WATER QUALITY PARAMETER DATA TABLES Sample Reach R01 R02 R03 R04 R05 R06 R07 R08 R09 R10 S am p le Location L01 L02 L03 L01 L02 L03 L01 L02 L03 L01 L02 L03 L01 L02 L03 L01 L02 L03 L01 L02 L03 L01 L02 L03 L01 L02 L03 L01 L02 L03 Appendix E Table 1: Surface Water - Fish Reaches Water Quality Parameters Sam ple ID DAL-SW-R01L1-0-121116 Date T im e 11/16/2012 10:45 Total Depth (feet) 18 DAL-SW-R01L2-0-121116 11/16/2012 11:15 13.5 DAL-SW-R01L3-0-121116 DAL-SW-R02L1-0-121116 DAL-SW-R02L2-0-121116 DAL-SW-R02L3-0-121116 DAL-SW-R03L1-0-121116 DAL-SW-R03L2-0-121116 DAL-SW-R03L3-0-121116 DAL-SW-R04L1-0-121116 DAL-SW-R04L2-0-121116 DAL-SW-R04L3-0-121116 DAL-SW-R05L1-0-121119 DAL-SW-R05L2-0-121119 DAL-SW-R05L3-0-121119 DAL-SW-R06L1-0-121120 DAL-SW-R06L2-0-121120 DAL-SW-R06L3-0-121120 DAL-SW-R07L1-0-121119 11/16/2012 11:40 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/16/2012 11/19/2012 11/19/2012 11/19/2012 11/20/2012 11/20/2012 11/20/2012 12:50 13:05 13:20 13:50 14:00 14:08 14:45 15:00 15:10 16:25 16:35 16:45 16:15 16:30 16:40 11/19/2012 15:10 13.4 6.3 4.6 7.0 3.6 4.0 2.5 2.0 7.0 2.0 5.5 5.2 3.0 1.4 3.7 4.1 13.5 DAL-SW-R07L2-0-121119 DAL-SW-R07L3-0-121119 DAL-SW-R08L1-0-121119 DAL-SW-R08L2-0-121119 DAL-SW-R08L3-0-121119 DAL-SW-R09L1-0-121119 DAL-SW-R09L2-0-121119 DAL-SW-R09L3-0-121119 DAL-SW-R10L1-0-121119 DAL-SW-R10L2-0-121119 DAL-SW-R10L3-0-121119 11/19/2012 15:25 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 11/19/2012 15:40 14:05 14:20 14:35 11:40 11:50 12:05 11/19/2012 10:35 11/19/2012 10:48 11/19/2012 11:00 13.2 2.8 3.9 4.3 6.3 6.4 3.4 3.0 10.2 5.6 9.7 S am p le Interval 0.2 0.8 0.2 0.8 0.2 0.8 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.2 0.8 0.2 0.8 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.2 0.8 0.6 0.6 Sam ple Depth (feet) 3.6 14.4 2.7 10.8 2.7 10.7 3.8 2.8 4.2 2.2 2.4 1.5 1.2 4.2 1.2 3.3 3.1 1.8 0.85 2.2 2.45 2.7 10.8 2.6 10.5 1.7 2.4 2.6 3.8 3.9 2.0 1.8 2.0 8.1 3.4 5.8 Field Parameters Temp. (C) 14.23 13.86 13.84 13.98 14.27 13.88 14.19 14.02 13.90 14.46 14.17 14.77 13.35 14.01 12.52 14.05 13.70 13.54 15.57 14.78 14.38 14.24 14.21 14.08 13.98 14.58 15.08 14.51 13.93 13.07 14.2 14.85 12.87 13.02 13.3 13.48 SC (us/cm3) 191 192 191 191 191 191 197 196 197 190 189 187 188 196 189 208 202 208 351 197 192 209 214 212 212 200 188 190 184 216 225 220 185 185 187 189 DO 53.3 53.3 55.3 54.3 54.3 54.3 62.5 57.4 60.4 61.4 56.3 59.4 61.4 57.4 57.4 53.3 58.4 56.3 38.0 41.0 39.0 54.3 54.3 54.3 51.2 52.3 63.5 54.3 54.3 57.4 66.3 58.4 56.3 53.3 54.3 59.4 pH 7.76 7.75 7.72 7.70 7.74 7.72 7.84 7.92 7.97 7.90 7.92 7.94 8.41 8.35 8.65 7.89 7.86 7.86 8.09 8.10 8.09 8.30 8.10 8.00 7.94 7.95 8.56 8.22 8.50 8.05 7.95 7.97 8.45 8.43 8.30 8.20 ORP 169.2 125.4 169.5 139.2 143.0 188.5 6.4 165.7 181.2 161.0 200.0 181.1 184.5 197.6 203.4 192.5 186.8 205.9 182.9 156.1 172.8 164.1 177.3 190.5 200.5 204.1 110.3 155.9 205.7 149.4 146.4 139.2 127.0 121.6 121.2 123.2 Turb. (NTU) 1.8 1.8 2.0 2.0 2.1 2.3 163.7 6.9 6.8 2.7 2.5 5.3 11.1 6.2 14.7 NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR Appendix E Table 1 (continued): Surface Water - Fish Reaches Water Quality Parameters Sample Reach R11 R12 S am p le Location L01 L02 L03 L01 L02 L03 SampeJD DAL-SW-R11L1-0-121119 DAL-SW-R11L2-0-121119 DAL-SW-R11L3-0-121119 DAL-SW-R12L1-0-121117 DAL-SW-R12L2-0-121117 Date 11/19/2012 11/19/2012 11/19/2012 11/17/2012 T im e 09:35 09:45 10:00 16:20 11/17/2012 16:35 Total Depth (feet) 5.5 5.3 6.7 5.6 14.0 DAL-SW-R12L3-0-121117 11/17/2012 16:50 11.0 L01 DAL-SW-R13L1-0-121117 11/17/2012 15:15 30.6 R13 L02 DAL-SW-R13L2-0-121117 11/17/2012 15:35 13.0 L03 DAL-SW-R13L3-0-121117 11/17/2012 15:50 21.9 L01 DAL-SW-R14L1-0-121117 11/17/2012 11:10 6.3 R14 L02 DAL-SW-R14L2-0-121117 11/17/2012 11:30 8.8 L03 DAL-SW-R14L3-0-121117 11/17/2012 11:45 3.1 L01 DAL-SW-R15L1-0-121117 11/17/2012 12:25 28.5 R15 L02 DAL-SW-R15L2-0-121117 11/17/2012 12:55 9.3 L03 DAL-SW-R15L3-0-121117 11/17/2012 13:10 4.2 Notes: Temp = Temperature SC = Specific Conductivity DO = Dissolved Oxygen ORP = Oxidation Reduction Potential Turb = Turbidity NTU = Nephelometric turbidity units NR = Not recorded due to instrument malfunction S am p le Interval 0.6 0.6 0.6 0.6 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.6 0.6 0.6 0.2 0.8 0.6 0.6 Sam ple Depth (feet) 3.3 3.2 4.0 3.4 2.8 11.2 2.2 8.8 6.1 24.6 2.6 10.4 4.4 17.5 3.8 5.3 1.9 5.7 22.8 5.6 2.5 Field Parameters Temp. SC (C) (us/cm3) 11.86 210 12.54 11.98 14.08 13.77 13.94 13.21 13.33 14.22 13.93 13.85 13.83 13.36 13.52 13.65 213 199 187 186 186 186 186 196 193 190 191 193 193 224 13.99 14.19 14.98 14.77 193 191 197 196 14.54 195 14.48 195 DO 59.4 54.3 53.3 51.2 49.2 50.2 50.2 49.2 59.4 54.3 52.3 52.3 51.2 51.2 60.4 51.2 51.2 58.4 52.3 51.2 53.3 pH 8.50 8.24 118.8 8.15 8.17 8.20 8.22 8.24 8.10 8.11 8.11 8.13 8.08 8.06 6.85 7.44 7.57 7.84 7.89 7.97 8.07 ORP 128.3 133.0 8.65 192.6 210.7 209.4 217.3 215.1 153.1 160.3 176.3 180.9 186.6 199.7 201.0 157.6 158.4 152.5 186.6 187.5 181.3 Turb. (NTU) NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR Appendix E Table 2: Surface Water - Transects Water Quality Parameters Sample Location Sample ID Date Time T1-01 DAL-SW-T101-0-121203 12/3/2012 08:40 Total Depth (feet) 21 T1-02 DAL-SW-T102-0-121203 12/03/2012 09:00 19 T1-03 DAL-SW-T103-0-121203 12/3/2012 09:15 18 T1-04 DAL-SW-T104-0-121203 12/3/2012 09:30 20 T1-05 DAL-SW-T105-0-121203 12/3/2012 09:45 24 T1-06 DAL-SW-T106-0-121203 12/3/2012 09:55 23 T1-07 DAL-SW-T107-0-121203 12/3/2012 10:05 22 T1-08 DAL-SW-T108-0-121203 12/3/2012 10:10 18 T1-09 DAL-SW-T109-0-121203 12/3/2012 10:15 18 T1-10 T2-01 T2-02 DAL-SW-T110-0-121203 DAL-SW-T201-0-121203 DAL-SW-T202-0-121203 12/3/2012 12/3/2012 12/3/2012 10:25 09:30 09:10 17 5.8 22.8 T2-03 DAL-SW-T203-0-121203 12/3/2012 08:50 21.6 T2-04 T2-05 T2-06 T2-07 T2-08 T2-09 T2-10 DAL-SW-T204-0-121203 DAL-SW-T205-0-121203 DAL-SW-T206-0-121203 DAL-SW-T207-0-121203 DAL-SW-T208-0-121204 DAL-SW-T209-0-121204 DAL-SW-T210-0-121204 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/4/2012 12/4/2012 12/4/2012 08:30 09:50 10:05 10:15 10:05 11:10 10:45 21.1 1.2 4.5 1.3 0.25 1.5 1.5 Sample Interval 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.6 0.2 0.8 0.2 0.8 0.2 0.8 0.6 0.6 0.6 0.6 0.6 0.6 Sample Depth (feet) 4.4 17.5 3.8 15.2 3.6 14.4 4 16 4.8 19.2 4.6 18.1 4.4 17.6 3.6 14.4 3.6 14.4 3.4 13.6 3.5 4.6 18.2 4.3 17.3 4.2 16.9 0.7 2.7 0.2 0.2 0.8 0.8 Temp. (C) 13.6 12.89 13.65 13.68 13.14 13.28 13.46 13.31 13.20 13.40 13.31 13.69 13.51 13.60 13.55 13.56 13.46 13.5 13.57 13.46 13.4 13.09 12.91 12.90 13.07 12.74 13.02 15.19 13.90 14.10 13.47 14.77 16.62 Field Parameters SC DO (ps/cm3) (mg/L) pH 198 49.5 24.7 205 46.9 23.7 196 53.0 26.7 198 49.6 25.7 196 52.7 25.7 196 49.2 25.7 195 101.8 46.7 196 43.0 23.7 195 107.7 45.1 195 105.5 44.1 194 109.3 44.1 195 101.1 43.1 194 98.1 40.0 194 101.9 42.0 193 84.5 36.9 194 89.3 38.0 118 93.4 40.0 195 97.0 41.0 194 97.9 41.0 192 96.1 41.0 240 58.4 7.77 242 57.4 7.72 239 57.4 7.75 233 54.3 7.82 236 121.2 8 302 57.4 8.24 233 55.3 8.08 237 59.4 7.84 238 56.9 7.80 237 58.4 7.72 194 2.29 8.14 169 31.6 8.62 193 3.69 8.40 ORP 8.09 8.66 7.97 7.99 7.91 7.92 7.9 7.95 7.87 7.88 7.84 7.84 7.83 7.81 7.79 7.81 7.75 7.77 7.71 7.72 173.7 183.2 179.0 173.0 159.4 177.6 183.7 168.9 175.8 172.8 152.4 179.4 170.2 Turb. (NTU) NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR 9.3 6.6 7.4 2.6 3.5 5.1 29 22.6 9.9 8.4 19.1 14.0 1.5 Appendix E Table 2 (continued): Surface Water - Transects Water Quality Parameters Sample Location Sample ID T3-01 DAL-SW-T301-0-121203 T3-02 DAL-SW-T302-0-121203 Date 12/3/2012 12/3/2012 Time 12:20 12:10 Total Depth (feet) 2.5 23.2 T3-03 T3-04 T3-05 T3-06 T3-07 T3-08 T3-09 T3-10 T4-01 T4-02 T4-03 T4-04 T4-05 T4-06 T4-07 T4-08 T4-09 T4-10 DAL-SW-T303-0-121203 12/3/2012 11:50 DAL-SW-T304-0-121203 DAL-SW-T305-0-121203 DAL-SW-T306-0-121203 DAL-SW-T307-0-121203 DAL-SW-T308-0-1212-03 DAL-SW-T309-0-121203 DAL-SW-T310-0-121203 DAL-SW-T401-0-121203 DAL-SW-T402-0-121203 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/03/2012 12/3/2012 12/3/2012 12:35 12:45 12:55 13:02 13:08 13:10 13:18 15:55 16:15 DAL-SW-T403-0-121203 12/3/2012 14:05 DAL-SW-T404-0-121203 DAL-SW-T405-0-121203 DAL-SW-T406-0-121203 DAL-SW-T407-0-121203 DAL-SW-T408-0-121203 DAL-SW-T409-0-121203 DAL-SW-T410-0-121203 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 12/3/2012 14:50 15:08 15:25 15:37 15:22 15:10 14:55 24.5 1.3 5.3 3.3 4.51 4.0 4.8 2.0 7.3 2.5 28 1.9 3.2 5.7 4.2 4.5 2.7 2.5 Sample Interval 0.6 0.2 0.8 0.2 0.8 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.2 0.8 0.6 0.6 0.6 0.6 0.6 0.6 0.6 Sample Depth (feet) 1.5 4.6 18.6 4.9 19.6 0.78 3.2 2.0 2.7 2.4 2.9 1.2 4.4 1.5 5.6 22.4 1.1 1.9 3.4 2.7 2.7 1.6 1.5 Temp. (C) 16.22 14.13 14.17 14.18 14.79 15.34 15.15 14.54 15.26 15.07 15.12 15.78 14.67 13.82 13.53 13.91 15.45 15.48 15.09 14.67 14.70 14.80 16.25 Field Parameters SC DO (ps/cm3) (mg/L) pH 239 44.1 7.88 238 52.3 7.79 242 174.6 7.72 242 53.3 7.61 250 55.3 7.74 236 41.0 7.92 232 42.0 7.84 231 41.0 7.85 231 41.0 7.86 231 41.0 7.94 231 40.0 7.92 230 40.0 8.05 244 38.0 7.65 202 27.7 8.31 236 39.0 7.76 244 38.0 7.86 230 39.0 7.66 230 41.0 7.77 178 37.8 7.58 192 29.1 8.36 193 26.9 8.48 191 32.8 8.64 218 32.0 8.61 ORP 167.5 191.2 175.7 174.0 161.2 187.4 176.0 173.6 164.8 181.3 176.5 177.7 174.3 164.2 170.7 127.7 165.4 161.3 202.5 198.7 180.2 163.3 128.5 Turb. (NTU) 6.6 3.1 4.9 2.9 2.5 5.1 5.9 8.2 15.4 19.3 20.6 26 6.7 NR 2.2 3.5 2.9 122 1.8 NR NR NR NR Appendix E Table 2 (continued): Surface Water - Transects Water Quality Parameters Sample Location Sample ID T5-01 DAL-SW-T501-0-121204 T5-02 DAL-SW-T502-0-121204 T5-03 DAL-SW-T503-0-121204 Date 12/4/2012 12/4/2012 12/4/2012 Time 11:00 10:50 10:20 Total Depth (feet) 1.5 4.5 28.0 T5-04 T5-05 T5-06 T5-07 T5-08 T5-09 T5-10 T6-01 T6-02 T6-03 T6-04 DAL-SW-T504-0-121204 2/4/2012 DAL-SW-T505-0-121204 DAL-SW-T506-0-121204 DAL-SW-T507-0-121204 DAL-SW-T508-0-121204 DAL-SW-T509-0-121204 DAL-SW-T510-0-121204 DAL-SW-T601-0-121204 DAL-SW-T602-0-121204 DAL-SW-T603-0-121204 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 DAL-SW-T604-0-121204 12/4/2012 10:35 11:22 11:30 11:38 11:50 11:57 12:05 13:40 13:32 13:26 13:20 23.5 5.3 7.0 5.4 7.2 6.4 3.8 3.9 6.5 4.2 23.7 T6-05 T6-06 T6-07 T6-08 T6-09 T6-10 DAL-SW-T605-0-121204 12/04/2012 13:10 DAL-SW-T606-0-121204 DAL-SW-T607-0-121204 DAL-SW-T608-0-121204 DAL-SW-T609-0-121204 DAL-SW-T610-0-121204 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12/4/2012 12:55 12:47 12:40 12:35 12:30 23.8 7.6 4.5 7.6 8.4 2.2 Sample Interval 0.6 0.6 0.2 0.8 0.2 0.8 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.2 0.8 0.2 0.8 0.6 0.6 0.6 0.6 0.6 Sample Depth (feet) 0.9 2.7 5.6 22.4 4.7 18.8 3.2 4.2 3.2 4.3 3.8 2.3 2.3 3.9 2.5 4.7 19.0 4.8 19.0 4.6 2.7 4.6 5.0 1.3 Temp. (C) 14.96 14.53 13.41 13.87 13.78 13.40 14.73 14.16 14.65 14.93 15.04 15.21 15.35 14.36 14.39 13.86 13.57 14.23 14.02 15.04 15.03 14.71 14.65 15.28 Field Parameters SC DO (ps/cm3) (mg/L) pH 244 36.9 7.72 242 36.9 7.69 237 36.9 7.69 240 34.9 7.78 231 39.0 7.66 232 36.9 7.70 232 39.0 7.79 231 38.0 7.79 232 36.9 7.84 230 39.0 7.86 229 40.0 7.92 226 41.0 8.02 249 43.1 8.36 249 39.0 7.97 237 36.9 7.87 233 36.9 7.80 234 38.0 7.86 232 39.0 7.86 235 38.0 7.94 231 40.0 8.08 230 39.0 8.08 229 38.0 8.06 230 41.0 8.19 229 39.0 8.09 ORP 171.8 177.3 172.6 152.4 178.4 176.3 165.7 140.6 157.3 169.8 164.8 170.8 174.4 171.4 177.1 184.4 165.4 175.8 164.6 202.6 16.01 157.8 167.1 165.7 Turb. (NTU) 6.8 4.8 3.7 4.3 3.4 3.5 3.3 4.3 6.6 9.0 15.1 33.7 29.0 8.3 5.5 3.3 3.7 5.8 4.2 10.2 15.0 22.1 31.8 31.3 Appendix E Table 2 (continued): Surface Water - Transects Water Quality Parameters Sample Location Sample ID T7-01 DAL-SW-T701-0-121204 T7-02 DAL-SW-T702-0-121204 Date 12/4/2012 12/4/2012 Time 14:35 14:45 Total Depth (feet) 2 11 T7-03 T7-04 T7-05 DAL-SW-T703-0-121204 12/4/2012 DAL-SW-T704-0-121204 12/4/2012 DAL-SW-T705-0-121204 12/4/2012 15:00 15:10 15:20 11 8.4 20 T7-06 DAL-SW-T706-0-121204 12/4/2012 15:20 20.6 T7-07 DAL-SW-T707-0-121204 12/4/2012 15:10 19.4 T7-08 T7-09 T7-10 T8-01 DAL-SW-T708-0-121204 12/4/2012 15:00 DAL-SW-T709-0-121204 DAL-SW-T710-0-121204 DAL-SW-T801-0-121205 12/4/2012 12/4/2012 12/5/2012 14:53 14:45 09:00 20.8 9.9 8.5 33.5 T8-02 DAL-SW-T802-0-121205 12/5/2012 09:08 42.5 T8-03 DAL-SW-T803-0-121205 12/5/2012 09:20 44.0 T8-04 DAL-SW-T804-0-121205 12/5/2012 09:30 43.5 T8-05 DAL-SW-T805-0-121205 12/05/2012 09:40 44.2 T8-06 DAL-SW-T806-0-121205 12/05/2012 09:52 42.7 T8-07 DAL-SW-T807-0-121205 12/5/2012 10:00 38.3 T8-08 DAL-SW-T808-0-121205 12/5/2012 10:10 28.0 T8-09 T8-10 DAL-SW-T809-0-121205 12/5/2012 DAL-SW-T810-0-121205 12/5/2012 10:23 10:35 25.2 2.8 Sample Interval 0.6 0.2 0.8 0.2 0.8 0.6 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.6 0.6 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.6 Sample Depth (feet) 1.2 2.2 8.8 2.2 8.8 4.8 4 16 4.1 16.5 3.9 15.5 4.2 16.6 5.9 5.1 6.7 26.8 8.5 34 8.8 35.2 8.7 34.8 8.8 35.4 8.5 34.2 7.7 30.6 5.6 22.4 5.0 20.2 1.7 Temp. (C) 15.13 - 15.49 14.89 14.84 15.74 15.76 14.61 15.49 15.31 15.55 15.38 16.68 16.01 17.07 17.36 13.77 13.60 13.84 13.53 13.79 13.37 13.85 13.28 13.85 13.26 13.89 13.16 13.91 13.15 14.02 13.54 13.91 13.78 14.06 Field Parameters SC DO (ps/cm3) (mg/L) pH 203 271 8.88 - -- 205 1.23 8.27 107 1.87 8.24 202 1.93 8.17 198 2.10 8.12 109 2.17 8.08 109 2.17 8.04 233 38.0 7.82 233 38.0 7.96 233 39.0 7.88 234 36.9 7.87 231 39.0 8.02 233 39.0 7.97 233 39.0 8.10 237 39.0 8.18 227 34.9 8.05 228 34.9 7.97 224 35.9 7.98 227 34.9 7.97 222 36.9 7.82 228 35.9 7.86 222 35.9 7.79 234 35.9 7.90 223 38.0 7.83 239 38.0 7.92 226 36.9 7.88 257 35.9 7.80 228 34.9 7.87 265 34.9 7.73 223 36.9 7.81 227 35.9 8.12 224 36.9 7.87 223 38.0 7.97 223 38.0 8.00 ORP 128.5 - 166.5 182.7 187.2 192.3 201.9 204.3 212.4 208.8 195.2 181.4 177.3 182.9 171.6 160.7 147.3 163.0 151.8 145.0 150.3 140.6 142.3 138.7 147.2 140.6 155.2 157.2 153.5 159.8 154.1 153.2 151.0 141.7 142.2 Turb. (NTU) NR - NR 17.3 18.3 17.6 NR NR 6.6 7.6 8.4 8.9 9.8 9.6 11.3 10.2 5.9 5.5 6.1 5.9 6.1 5.4 6.4 6.0 6.4 6.7 6.6 9.2 6.6 9.0 6.6 10.3 6.6 7.6 7.0 Appendix E Table 2 (continued): Surface Water - Transects Water Quality Parameters Sample Location Sample ID T9-01 DAL-SW-T901-0-121205 Date 12/5/2012 Time 10:40 Total Depth (feet) 55 T9-02 DAL-SW-T902-0-121205 12/5/2012 11:10 60 T9-03 DAL-SW-T903-0-121205 12/05/2012 11:15 60 T9-04 DAL-SW-T904-0-121205 12/5/2012 11:30 60 T9-05 DAL-SW-T905-0-121205 12/5/2012 11:45 60 T9-06 DAL-SW-T906-0-121205 12/5/2012 12:00 62 T9-07 DAL-SW-T907-0-121205 12/5/2012 12:10 63 T9-08 DAL-SW-T908-0-121205 12/5/2012 12:30 67 T9-09 DAL-SW-T909-0-121205 12/5/2012 13:10 68 T9-10 DAL-SW-T910-0-121205 12/5/2012 13:10 60 Notes: Temp = Temperature SC = Specific Conductivity DO = Dissolved Oxygen ORP = Oxidation Reduction Potential Turb = Turbidity NTU = Nephelometric turbidity units - = not recorded NR = Not recorded due to instrument malfunction Sample Interval 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 0.2 0.8 Sample Depth (feet) 11 44 12 48 12 48 12 48 12 48 12.4 48.8 12.6 50.4 13.4 53.6 13.6 54.4 12 48 Temp. (C) 14.16 13.87 13.72 13.45 13.53 13.31 13.74 13.57 13.52 13.37 13.57 13.76 13.81 14.11 15.87 14.19 13.68 14.08 13.87 Field Parameters SC DO (ps/cm3) (mg/L) pH 197 1.46 8.01 198 1.59 7.98 197 1.71 7.98 196 1.81 7.99 194 1.89 8.02 185 1.78 7.98 193 1.82 8.04 - -- 197 1.71 8.05 198 1.76 7.95 196 1.86 7.98 198 1.91 7.93 197 1.99 7.98 198 1.74 7.99 197 1.71 7.98 197 1.76 7.99 196 1.66 7.95 196 1.75 8.02 196 1.80 8.02 1.95 1.81 8.05 ORP 182.1 183.4 182.8 181.0 169.4 164.7 173.1 158.3 154.6 150.9 151.5 144.4 149.2 151.1 161.4 163.9 177.0 177.9 180.0 Turb. (NTU) NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR APPENDIX F STATISTICAL OUTPUTS APPENDIX F-1 LARGEMOUTH BASS STATISTICS TEMPORAL EVALUATION 4/10/2013 10:38:27 AM 1 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R01, Date=Jul-07 6 72.18333 44.53688 18.1821 25.44474 118.9219 Reach=R01, Date=Jul-08 6 59.27833 20.29346 8.284771 37.98165 80.57502 Note: T-alpha (R each=R 01, Date=Jul-07) = 2.5706, T-alpha (Reach=R01, Date=Jul-08) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 12.905 34.6075 Unequal 6.99 12.905 48.9424 Note: T-alpha (Equal) = 2.2281, T-alpha (Unequal) = 2.3653 Standard Error 19.98065 19.98065 95.0% LCL Difference -31.61466 -34.35486 95.0% UCL Difference 57.42466 60.16486 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6459 0.532901 No Difference < 0 0.6459 0.733550 No Difference > 0 0.6459 0.266450 No Difference: (Reach=R01, Date=Jul-07)-(Reach=R01, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.090179 0.012246 0.148737 Power (Alpha=.010) 0.022187 0.001899 0.039044 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6459 0.538976 No Difference < 0 0.6459 0.730512 No Difference > 0 0.6459 0.269488 No Difference: (Reach=R01, Date=Jul-07)-(Reach=R01, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.086876 0.012775 0.144647 Power (Alpha=.010) 0.020525 0.002075 0.036490 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R01, Date=Jul-07) Kurtosis Normality (Reach=R01, Date=Jul-07) Omnibus Normality (Reach=R01, Date=Jul-07) Skewness Normality (Reach=R01, Date=Jul-08) Kurtosis Normality (Reach=R01, Date=Jul-08) Omnibus Normality (Reach=R01, Date=Jul-08) Variance-Ratio Equal-Variance Test 4.8165 Modified-Levene Equal-Variance Test 2.4632 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.109474 0.147610 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-07 Reach=R01, Date=Jul-08 Count 6 6 Median 65.5 59.105 95.0% LCL of Median 22.9 26.38 95.0% UCL of Median 145.7 81.38 4/10/2013 10:38:28 AM 2 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01, Date=Jul-07 Reach=R01, Date=Jul-08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 19 40 17 38 Multiplicity Factor = 0 Mean of W 39 39 Std Dev of W 6.244998 6.244998 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Approx. Without Correction Prob Reject H0 Prob Reject H0 Level at .050 Z-Value Level at .050 0.937229 No 0.1601 0.872780 No 0.531385 No 0.1601 0.563610 No 0.468615 No 0.1601 0.436390 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.0801 0.936186 No 0.2402 0.594909 No 0.0801 0.468093 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.333333 0.166667 0.333333 Reject H0 if Greater Than 0.6980 0.6980 0.6980 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.9307 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:38:28 AM 3 4/10/2013 10:38:28 AM 4 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R01, Date=Jul-07 6 72.18333 44.53688 18.1821 25.44474 118.9219 Reach=R01, Date=Jul-09 6 53.44333 27.72979 11.32064 24.3427 82.54396 Note: T-alpha (R each=R 01, Date=Jul-07) = 2.5706, T-alpha (Reach=R01, Date=Jul-09) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 18.74 37.09768 Unequal 8.37 18.74 52.46404 Note: T-alpha (Equal) = 2.2281, T-alpha (Unequal) = 2.2884 Standard Error 21.41835 21.41835 95.0% LCL Difference -28.98306 -30.27319 95.0% UCL Difference 66.46307 67.75319 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.8750 0.402126 No Difference < 0 0.8750 0.798937 No Difference > 0 0.8750 0.201063 No Difference: (Reach=R01, Date=Jul-07)-(Reach=R01, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.124655 0.006869 0.203734 Power (Alpha=.010) 0.033766 0.000978 0.059077 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.8750 0.406006 No Difference < 0 0.8750 0.796997 No Difference > 0 0.8750 0.203003 No Difference: (Reach=R01, Date=Jul-07)-(Reach=R01, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.121761 0.007063 0.200597 Power (Alpha=.010) 0.032131 0.001037 0.056785 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R01, Date=Jul-07) Kurtosis Normality (Reach=R01, Date=Jul-07) Omnibus Normality (Reach=R01, Date=Jul-07) Skewness Normality (Reach=R01, Date=Jul-09) Kurtosis Normality (Reach=R01, Date=Jul-09) Omnibus Normality (Reach=R01, Date=Jul-09) Variance-Ratio Equal-Variance Test 2.5796 Modified-Levene Equal-Variance Test 0.9324 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.321625 0.357002 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-07 Reach=R01, Date=Jul-09 Count 6 6 Median 65.5 48.465 95.0% LCL of Median 22.9 19.85 95.0% UCL of Median 145.7 89.13 4/10/2013 10:38:28 AM 5 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01, Date=Jul-07 Reach=R01, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 23 44 13 34 Multiplicity Factor = 0 Mean of W 39 39 Std Dev of W 6.244998 6.244998 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.484848 No 0.757576 No 0.242424 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.8006 0.423340 No 0.8006 0.788330 No 0.8006 0.211670 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.7206 0.471170 No 0.8807 0.810761 No 0.7206 0.235585 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.333333 0.000000 0.333333 Reject H0 if Greater Than 0.6980 0.6980 0.6980 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.9307 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:38:28 AM 6 4/10/2013 10:38:28 AM 7 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R01, Date=Jul-07 6 72.18333 44.53688 18.1821 25.44474 118.9219 Reach=R01, Date=Nov-12 5 30.56 7.657872 3.424704 21.0515 Note: T-alpha (Reach=R01, Date=Jul-07) = 2.5706, T-alpha (Reach=R01, Date =Nov-12) = 2.7764 40.0685 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 41.62333 33.58611 Unequal 5.35 41.62333 45.19045 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 2.5205 Standard Error 20.3374 18.50183 95.0% LCL Difference -4.383059 -5.009931 95.0% UCL Difference 87.62972 88.2566 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0466 0.070999 No Difference < 0 2.0466 0.964501 No Difference > 0 2.0466 0.035499 Yes Difference: (Reach=R01, Date=Jul-07)-(Reach=R01, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.447615 0.000191 0.596756 Power (Alpha=.010) 0.187444 0.000019 0.281338 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2497 0.070802 No Difference < 0 2.2497 0.964599 No Difference > 0 2.2497 0.035401 Yes Difference: (Reach=R01, Date=Jul-07)-(Reach=R01, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.455168 0.000127 0.622207 Power (Alpha=.010) 0.169057 0.000016 0.269453 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R01, Date=Jul-07) Kurtosis Normality (Reach=R01, Date=Jul-07) Omnibus Normality (Reach=R01, Date=Jul-07) Skewness Normality (Reach=R01, Date=Nov-12) Kurtosis Normality (Reach=R01, Date=Nov-12) Omnibus Normality (Reach=R01, Date=Nov-12) Variance-Ratio Equal-Variance Test 33.8239 Modified-Levene Equal-Variance Test 5.3498 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.004564 0.046013 Reject equal variances Reject equal variances Median Statistics Variable Reach=R01, Date=Jul-07 Reach=R01, Date=Nov-12 Count 6 5 Median 65.5 32.7 95.0% LCL of Median 22.9 95.0% UCL of Median 145.7 4/10/2013 10:38:28 AM 8 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01, Date=Jul-07 Reach=R01, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 46 5 20 Multiplicity Factor = 0 Mean of W 36 30 Std Dev of W 5.477226 5.477226 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.082251 No 0.958874 No 0.041126 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.8257 0.067889 No -1.8257 0.966055 No -1.8257 0.033945 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.7345 0.082837 No -1.9170 0.972383 No -1.7345 0.041419 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.666667 0.000000 0.666667 Reject H0 if Greater Than 0.7246 0.7246 0.7246 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.1082 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:38:28 AM 9 Normal Probability Plot of Largemouth_Bass_FFOS_ppb when Reacn- R01, Date- Percent of Values 4/10/2013 10:38:38 AM 109 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R01, Date=Jul-08 6 59.27833 20.29346 8.284771 37.98165 80.57502 Reach=R01, Date=Jul-09 6 53.44333 27.72979 11.32064 24.3427 82.54396 Note: T-alpha (R each=R 01, Date=Jul-08) = 2.5706, T-alpha (Reach=R01, Date=Jul-09) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 5.835 24.2978 Unequal 9.16 5.835 34.36227 Note: T-alpha (Equal) = 2.2281, T-alpha (Unequal) = 2.2561 Standard Error 14.02834 14.02834 95.0% LCL Difference -25.42209 -25.81402 95.0% UCL Difference 37.09209 37.48402 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.4159 0.686237 No Difference < 0 0.4159 0.656882 No Difference > 0 0.4159 0.343118 No Difference: (Reach=R01, Date=Jul-08)-(Reach=R01, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.066484 0.020978 0.104493 Power (Alpha=.010) 0.014825 0.003554 0.024857 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.4159 0.687025 No Difference < 0 0.4159 0.656487 No Difference > 0 0.4159 0.343513 No Difference: (Reach=R01, Date=Jul-08)-(Reach=R01, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.066191 0.021095 0.104053 Power (Alpha=.010) 0.014682 0.003597 0.024607 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R01, Date=Jul-08) Kurtosis Normality (Reach=R01, Date=Jul-08) Omnibus Normality (Reach=R01, Date=Jul-08) Skewness Normality (Reach=R01, Date=Jul-09) Kurtosis Normality (Reach=R01, Date=Jul-09) Omnibus Normality (Reach=R01, Date=Jul-09) Variance-Ratio Equal-Variance Test 1.8672 Modified-Levene Equal-Variance Test 0.6163 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.509711 0.450620 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-08 Reach=R01, Date=Jul-09 Count 6 6 Median 59.105 48.465 95.0% LCL of Median 26.38 19.85 95.0% UCL of Median 81.38 89.13 4/10/2013 10:38:38 AM 110 Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01, Date=Jul-08 Reach=R01, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 41 16 37 Multiplicity Factor = 0 Mean of W 39 39 Std Dev of W 6.244998 6.244998 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.818182 No 0.590909 No 0.409091 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.3203 0.748774 No 0.3203 0.625613 No 0.3203 0.374387 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.2402 0.810181 No 0.4003 0.655540 No 0.2402 0.405091 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.333333 0.333333 0.333333 Reject H0 if Greater Than 0.6980 0.6980 0.6980 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.9307 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:38:38 AM 111 4/10/2013 10:38:38 AM 112 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R01, Date=Jul-08 6 59.27833 20.29346 8.284771 37.98165 80.57502 Reach=R01, Date=Nov-12 5 30.56 7.657872 3.424704 21.0515 Note: T-alpha (Reach=R01, Date=Jul-08) = 2.5706, T-alpha (Reach=R01, Date =Nov-12) = 2.7764 40.0685 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 28.71833 15.9641 8 Unequal 6.61 28.71833 21.69027 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 2.3929 Standard Error 9.66679 8.96471 95.0% LCL Difference 6.850534 7.266344 95.0% UCL Difference 50.58613 50.17032 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9708 0.015681 Yes Difference < 0 2.9708 0.992159 No Difference > 0 2.9708 0.007841 Yes Difference: (Reach=R01, Date=Jul-08)-(Reach=R01, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.753124 0.000005 0.862676 Power (Alpha=.010) 0.443275 0.000000 0.577752 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.2035 0.016197 Yes Difference < 0 3.2035 0.991902 No Difference > 0 3.2035 0.008098 Yes Difference: (Reach=R01, Date=Jul-08)-(Reach=R01, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.776059 0.000002 0.886736 Power (Alpha=.010) 0.439366 0.000000 0.587031 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R01, Date=Jul-08) Kurtosis Normality (Reach=R01, Date=Jul-08) Omnibus Normality (Reach=R01, Date=Jul-08) Skewness Normality (Reach=R01, Date=Nov-12) Kurtosis Normality (Reach=R01, Date=Nov-12) Omnibus Normality (Reach=R01, Date=Nov-12) Variance-Ratio Equal-Variance Test 7.0226 Modified-Levene Equal-Variance Test 2.4871 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.082337 0.149238 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-08 Reach=R01, Date=Nov-12 Count 6 5 Median 59.105 32.7 95.0% LCL of Median 26.38 95.0% UCL of Median 81.38 4/10/2013 10:38:38 AM 113 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01, Date=Jul-08 Reach=R01, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 26 47 4 19 Multiplicity Factor = 0 Mean of W 36 30 Std Dev of W 5.477226 5.477226 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.051948 No 0.974026 No 0.025974 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.0083 0.044610 Yes -2.0083 0.977695 No -2.0083 0.022305 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.9170 0.055234 No -2.0996 0.982118 No -1.9170 0.027617 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.833333 0.000000 0.833333 Reject H0 if Greater Than 0.7246 0.7246 0.7246 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0260 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:38:38 AM 114 4/10/2013 10:38:47 AM 214 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R01, Date=Jul-09 6 53.44333 27.72979 11.32064 24.3427 82.54396 Reach=R01, Date=Nov-12 5 30.56 7.657872 3.424704 21.0515 Note: T-alpha (Reach=R01, Date=Jul-09) = 2.5706, T-alpha (Reach=R01, Date =Nov-12) = 2.7764 40.0685 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 22.88333 21.28974 Unequal 5.90 22.88333 28.76777 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 2.4575 Standard Error 12.89158 11.82732 95.0% LCL Difference -6.279446 -6.182082 95.0% UCL Difference 52.04611 51.94875 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.7751 0.109628 No Difference < 0 1.7751 0.945186 No Difference > 0 1.7751 0.054814 No Difference: (Reach=R01, Date=Jul-09)-(Reach=R01, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.354830 0.000486 0.498098 Power (Alpha=.010) 0.133894 0.000053 0.209474 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.9348 0.102028 No Difference < 0 1.9348 0.948986 No Difference > 0 1.9348 0.051014 No Difference: (Reach=R01, Date=Jul-09)-(Reach=R01, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.368560 0.000350 0.525452 Power (Alpha=.010) 0.128541 0.000044 0.209262 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R01, Date=Jul-09) Kurtosis Normality (Reach=R01, Date=Jul-09) Omnibus Normality (Reach=R01, Date=Jul-09) Skewness Normality (Reach=R01, Date=Nov-12) Kurtosis Normality (Reach=R01, Date=Nov-12) Omnibus Normality (Reach=R01, Date=Nov-12) Variance-Ratio Equal-Variance Test 13.1122 Modified-Levene Equal-Variance Test 4.3923 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.027287 0.065567 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-09 Reach=R01, Date=Nov-12 Count 6 5 Median 48.465 32.7 95.0% LCL of Median 19.85 95.0% UCL of Median 89.13 4/10/2013 10:38:47 AM 215 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01, Date=Jul-09 Reach=R01, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 23 44 7 22 Multiplicity Factor = 0 Mean of W 36 30 Std Dev of W 5.477226 5.477226 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.177489 No 0.911255 No 0.088745 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.4606 0.144127 No -1.4606 0.927936 No -1.4606 0.072064 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.3693 0.170904 No -1.5519 0.939655 No -1.3693 0.085452 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.666667 0.000000 0.666667 Reject H0 if Greater Than 0.7246 0.7246 0.7246 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.1082 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Nomai Probability Plot of _argemouth_Eass_PFQS_ppb when Reach~RC1, Jate~ 4/10/2013 10:38:47 AM 216 1 90 n 5 10 25 50 75 Percent of Values 90 95 Largemouth_Bass_FFOS_ppb vs Groups 99 4/10/2013 10:39:06 AM 415 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R02, Date=Jun-02 7 2.5 0 0 2.5 2.5 Reach=R02, Date=Nov-04 5 103.68 24.48207 10.94872 73.28149 134.0785 Note: T-alpha (Reach=R02, Date=Jun-02) = 0.0000, T-alpha (Reach=R02, Date=Nov-04) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -101.18 15.48382 Unequal 4.00 -101.18 24.48207 Note: T-alpha (Equal) = 2.2281, T-alpha (Unequal) = 2.7764 Standard Error 9.066399 10.94872 95.0% LCL Difference -121.3812 -131.5785 95.0% UCL Difference -80.97881 -70.78149 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -11.1599 0.000001 Yes Difference < 0 -11.1599 0.000000 Yes Difference > 0 -11.1599 1.000000 No Difference: (Reach=R02, Date=Jun-02)-(Reach= R02, Date= Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 1.000000 1.000000 0.000000 Power (Alpha=.010) 1.000000 1.000000 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -9.2413 0.000762 Yes Difference < 0 -9.2413 0.000381 Yes Difference > 0 -9.2413 0.999619 No Difference: (Reach=R02, Date=Jun-02)-(Reach=R02, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999996 1.000000 0.000000 Power (Alpha=.010) 0.992921 0.999422 0.000000 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R02, Date=Jun-02) Kurtosis Normality (Reach=R02, Date=Jun-02) Omnibus Normality (Reach=R02, Date=Jun-02) Skewness Normality (Reach=R02, Date=Nov-04) Kurtosis Normality (Reach=R02, Date=Nov-04) Omnibus Normality (Reach=R02, Date=Nov-04) Variance-Ratio Equal-Variance Test 0.0000 Modified-Levene Equal-Variance Test 7.8194 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.000000 0.018914 Reject equal variances Reject equal variances Median Statistics Variable Reach=R02, Date=Jun-02 Reach=R02, Date=Nov-04 Count 7 5 Median 2.5 114 95.0% LCL of Median 2.5 95.0% UCL of Median 2.5 4/10/2013 10:39:07 AM 416 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02, Date=Jun-02 Reach=R02, Date=Nov-04 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 28 35 50 Multiplicity Factor = 336 Mean of W 45.5 32.5 Std Dev of W 5.521995 5.521995 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Approx. With Correction Prob Reject H0 Prob Reject H0 Z-Value Level at .050 Z-Value Level at .050 3.1691 0.001529 Yes 3.0786 0.002080 Yes 3.1691 0.000764 Yes 3.0786 0.001040 Yes 3.1691 0.999236 No 3.2597 0.999442 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7061 0.7061 0.7061 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0025 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:39:07 AM 41 7 4/10/2013 10:39:07 AM 418 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R02, Date=Jun-02 7 2.5 0 0 2.5 2.5 Reach=R02, Date=Dec-06 5 34.24 24.16574 10.80725 4.234271 64.24573 Note: T-alpha (Reach=R02, Date=Jun-02) = 0.0000, T-alpha (Reach=R02, Date=D ec-06) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -31.74 15.28376 Unequal 4.00 -31.74 24.16574 Note: T-alpha (Equal) = 2.2281, T-alpha (Unequal) = 2.7764 Standard Error 8.949251 10.80725 95.0% LCL Difference -51.68018 -61.74573 95.0% UCL Difference -11.79983 -1.73427 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.5467 0.005298 Yes Difference < 0 -3.5467 0.002649 Yes Difference > 0 -3.5467 0.997351 No Difference: (Reach=R02, Date=Jun-02)-(Reach= R02, Date= Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.890793 0.950344 0.000000 Power (Alpha=.010) 0.647896 0.767042 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.9369 0.042519 Yes Difference < 0 -2.9369 0.021260 Yes Difference > 0 -2.9369 0.978740 No Difference: (Reach=R02, Date=Jun-02)-(Reach=R02, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.601940 0.776464 0.000013 Power (Alpha=.010) 0.234375 0.371035 0.000002 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R02, Date=Jun-02) Kurtosis Normality (Reach=R02, Date=Jun-02) Omnibus Normality (Reach=R02, Date=Jun-02) Skewness Normality (Reach=R02, Date=Dec-06) Kurtosis Normality (Reach=R02, D ate=Dec-06) Omnibus Normality (Reach=R02, Date=Dec-06) Variance-Ratio Equal-Variance Test 0.0000 Modified-Levene Equal-Variance Test 4.9839 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.000000 0.049635 Reject equal variances Reject equal variances Median Statistics Variable Reach=R02, Date=Jun-02 Reach=R02, Date=Dec-06 Count 7 5 Median 2.5 19.6 95.0% LCL of Median 2.5 95.0% UCL of Median 2.5 4/10/2013 10:39:07 AM 419 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02, Date=Jun-02 Reach=R02, Date=Dec-06 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 28 35 50 Multiplicity Factor = 336 Mean of W 45.5 32.5 Std Dev of W 5.521995 5.521995 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Approx. With Correction Prob Reject H0 Prob Reject H0 Z-Value Level at .050 Z-Value Level at .050 3.1691 0.001529 Yes 3.0786 0.002080 Yes 3.1691 0.000764 Yes 3.0786 0.001040 Yes 3.1691 0.999236 No 3.2597 0.999442 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7061 0.7061 0.7061 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0025 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:39:07 AM 420 4/10/2013 10:39:07 AM 421 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R02, Date=Jun-02 7 2.5 0 0 2.5 2.5 Reach=R02, Date=Nov-12 5 43.2 6.932893 3.100484 34.59168 51.80832 Note: T-alpha (Reach=R02, Date=Jun-02) = 0.0000, T-alpha (Reach=R02, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -40.7 4.384746 Unequal 4.00 -40.7 6.932893 Note: T-alpha (Equal) = 2.2281, T-alpha (Unequal) = 2.7764 Standard Error 2.567445 3.100484 95.0% LCL Difference -46.42062 -49.30832 95.0% UCL Difference -34.97938 -32.09168 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -15.8523 0.000000 Yes Difference < 0 -15.8523 0.000000 Yes Difference > 0 -15.8523 1.000000 No Difference: (Reach=R02, Date=Jun-02)-(Reach=R02, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 1.000000 1.000000 0.000000 Power (Alpha=.010) 1.000000 1.000000 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -13.1270 0.000194 Yes Difference < 0 -13.1270 0.000097 Yes Difference > 0 -13.1270 0.999903 No Difference: (Reach=R02, Date=Jun-02)-(Reach=R02, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 1.000000 1.000000 0.000000 Power (Alpha=.010) 0.999987 1.000000 0.000000 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R02, Date=Jun-02) Kurtosis Normality (Reach=R02, Date=Jun-02) Omnibus Normality (Reach=R02, Date=Jun-02) Skewness Normality (Reach=R02, Date=Nov-12) Kurtosis Normality (Reach=R02, Date=Nov-12) Omnibus Normality (Reach=R02, Date=Nov-12) Variance-Ratio Equal-Variance Test 0.0000 Modified-Levene Equal-Variance Test 10.4542 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.000000 0.008971 Reject equal variances Reject equal variances Median Statistics Variable Reach=R02, Date=Jun-02 Reach=R02, Date=Nov-12 Count 7 5 Median 2.5 44.1 95.0% LCL of Median 2.5 95.0% UCL of Median 2.5 4/10/2013 10:39:07 AM 422 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02, Date=Jun-02 Reach=R02, Date=Nov-12 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 28 35 50 Multiplicity Factor = 336 Mean of W 45.5 32.5 Std Dev of W 5.521995 5.521995 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Approx. With Correction Prob Reject H0 Prob Reject H0 Z-Value Level at .050 Z-Value Level at .050 3.1691 0.001529 Yes 3.0786 0.002080 Yes 3.1691 0.000764 Yes 3.0786 0.001040 Yes 3.1691 0.999236 No 3.2597 0.999442 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7061 0.7061 0.7061 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0025 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:39:07 AM 423 4/10/2013 10:39:16 AM 511 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R02, Date=Nov-04 5 103.68 24.48207 10.94872 73.28149 134.0785 Reach=R02, Date=Dec-06 5 34.24 24.16574 10.80725 4.234271 64.24573 Note: T-alpha (Reach=R02, Date=Nov-04) = 2.7764, T-alpha (Reach=R02, Date=D ec-06) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 69.44 24.32442 Unequal 8.00 69.44 34.39993 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3061 Standard Error 15.38412 15.38412 95.0% LCL Difference 33.96417 33.96312 95.0% UCL Difference 104.9158 104.9169 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 4.5137 0.001966 Yes Difference < 0 4.5137 0.999017 No Difference > 0 4.5137 0.000983 Yes Difference: (Reach=R02, Date=Nov-04)-(Reach=R02, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.975394 0.000000 0.992853 Power (Alpha=.010) 0.834804 0.000000 0.916470 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 4.5137 0.001967 Yes Difference < 0 4.5137 0.999017 No Difference > 0 4.5137 0.000983 Yes Difference: (Reach=R02, Date=Nov-04)-(Reach=R02, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.975388 0.000000 0.992851 Power (Alpha=.010) 0.834765 0.000000 0.916449 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R02, Date=Nov-04) Kurtosis Normality (Reach=R02, Date=Nov-04) Omnibus Normality (Reach=R02, Date=Nov-04) Skewness Normality (Reach=R02, Date=Dec-06) Kurtosis Normality (Reach=R02, D ate=Dec-06) Omnibus Normality (Reach=R02, Date=Dec-06) Variance-Ratio Equal-Variance Test 1.0264 Modified-Levene Equal-Variance Test 0.0023 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.980494 0.962680 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02, Date=Nov-04 Reach=R02, Date=Dec-06 Count 5 5 Median 114 19.6 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:39:16 AM 512 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02, Date=Nov-04 Reach=R02, Date=Dec-06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:39:16 AM 513 Morirai Probability Plct of Largemou:h_Bass_PFOS_ppb when Rea:h=R02, Date=N 135 n Normal Probability Plot of Largemouth_Bass_FFOS_ppb when Reacn- R02, Date- Percent ofValues Largemouth_Bass_PFOS_ppbvs Groms 141-| Grouos 4/10/2013 10:39:16 AM 514 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R02, Date=Nov-04 5 103.68 24.48207 10.94872 73.28149 134.0785 Reach=R02, Date=Nov-12 5 43.2 6.932893 3.100484 34.59168 51.80832 Note: T-alpha (Reach=R02, Date=Nov-04) = 2.7764, T-alpha (Reach=R02, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 60.48 17.99218 Unequal 4.64 60.48 25.44478 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6322 Standard Error 11.37925 11.37925 95.0% LCL Difference 34.2394 30.52728 95.0% UCL Difference 86.7206 90.43272 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.3149 0.000715 Yes Difference < 0 5.3149 0.999642 No Difference > 0 5.3149 0.000358 Yes Difference: (Reach=R02, Date=Nov-04)-(Reach=R02, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.995943 0.000000 0.999227 Power (Alpha=.010) 0.942236 0.000000 0.978093 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.3149 0.003942 Yes Difference < 0 5.3149 0.998029 No Difference > 0 5.3149 0.001971 Yes Difference: (Reach=R02, Date=Nov-04)-(Reach=R02, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.982366 0.000000 0.997087 Power (Alpha=.010) 0.790494 0.000000 0.908495 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R02, Date=Nov-04) Kurtosis Normality (Reach=R02, Date=Nov-04) Omnibus Normality (Reach=R02, Date=Nov-04) Skewness Normality (Reach=R02, Date=Nov-12) Kurtosis Normality (Reach=R02, Date=Nov-12) Omnibus Normality (Reach=R02, Date=Nov-12) Variance-Ratio Equal-Variance Test 12.4700 Modified-Levene Equal-Variance Test 2.6757 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.031432 0.140529 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02, Date=Nov-04 Reach=R02, Date=Nov-12 Count 5 5 Median 114 44.1 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:39:16 AM 515 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02, Date=Nov-04 Reach=R02, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Norrral Probability Piet of Largemou:h_Bass_PFOS_ppb when Rea:h=R02, Date=N 135 n 4/10/2013 10:39:17 AM 516 Percent ofValues Largemouth_Bass_PFOS_ppbvs Groms 141 -i 30 J----------------------------- 1---------------------------------------------------------- 1------------------ Reach=R02, Cats=Ncv-04 Reach=R02, Date=Notf-12 Groms 4/10/2013 10:39:25 AM 604 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R02, Date=Dec-06 5 34.24 24.16574 10.80725 4.234271 64.24573 Reach=R02, Date=Nov-12 5 43.2 6.932893 3.100484 34.59168 51.80832 Note: T-alpha (Reach=R02, Date=D ec-06) = 2.7764, T-alpha (Reach=R02, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -8.96 17.77706 Unequal 4.65 -8.96 25.14056 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6291 Standard Error 11.2432 11.2432 95.0% LCL Difference -34.88687 -38.51993 95.0% UCL Difference 16.96687 20.59993 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.7969 0.448497 No Difference < 0 -0.7969 0.224249 No Difference > 0 -0.7969 0.775751 No Difference: (Reach=R02, D ate=D ec-06)-(R each=R 02, D ate:=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.108636 0.180358 0.008676 Power (Alpha=.010) 0.027639 0.049135 0.001317 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.7969 0.464187 No Difference < 0 -0.7969 0.232094 No Difference > 0 -0.7969 0.767906 No Difference: (Reach=R02, D ate=D ec-06)-(R each=R 02, D ate:=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.098955 0.168806 0.009634 Power (Alpha=.010) 0.023034 0.042072 0.001612 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R02, Date= Dec-06) Kurtosis Normality (Reach=R02, D ate=Dec-06) Omnibus Normality (Reach=R02, Date=Dec-06) Skewness Normality (Reach=R02, Date= Nov-12) Kurtosis Normality (Reach=R02, Date=Nov-12) Omnibus Normality (Reach=R02, Date=Nov-12) Variance-Ratio Equal-Variance Test 12.1499 Modified-Levene Equal-Variance Test 1.6908 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.032939 0.229708 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02, Date=Dec-06 Reach=R02, Date=Nov-12 Count 5 5 Median 19.6 44.1 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:39:26 AM 605 Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02, Date=Dec-06 Reach=R02, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 10 25 15 30 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.690476 No 0.345238 No 0.654762 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.5222 0.601508 No -0.5222 0.300754 No -0.5222 0.699246 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.4178 0.676103 No -0.4178 0.338052 No -0.6267 0.734565 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Nomai Probability Plot of _argemouth_Eass_PFQS_ppb when Reach~RC2, Jate~ 4/10/2013 10:39:26 AM 606 Largemouth_Bass_FFOS_ppbvs Groups Reach=R02 Date=Dec-03 Groups Rea:h=R02, Date=Nov-12 4/10/2013 10:40:04 AM 946 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Nov-04 10 3897.8 3255.094 1029.351 1569.246 6226.354 Reach=R05, Date=Dec-06 10 2726 2687.829 849.9661 803.2431 4648.757 Note: T-alpha (R e ac h =R05, Date=Nov-04) = 2.2622, T-alpha (Reach=R05, Date=D ec-06) = 2.2622 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 18 1171.8 2984.968 Unequal 17.38 1171.8 4221.382 Note: T-alpha (Equal) = 2.1009, T-alpha (Unequal) = 2.1063 Standard Error 1334.918 1334.918 95.0% LCL Difference -1632.759 -1639.971 95.0% UCL Difference 3976.359 3983.571 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.8778 0.391615 No Difference < 0 0.8778 0.804192 No Difference > 0 0.8778 0.195808 No II ZJ o en D CD ilCD Difference: (Reach=R05, Date= Nov-04)-(Reach Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.132225 0.006374 0.211899 Power (Alpha=.010) 0.038285 0.000837 0.065216 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.8778 0.392023 No Difference < 0 0.8778 0.803988 No Difference > 0 0.8778 0.196012 No Difference: (Reach=R05, Date=Nov-04)-(Reach=R05, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.131896 0.006394 0.211562 Power (Alpha=.010) 0.038072 0.000843 0.064937 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Nov-04) Kurtosis Normality (Reach=R05, Date=Nov-04) Omnibus Normality (Reach=R05, Date=Nov-04) Skewness Normality (Reach=R05, Date=Dec-06) Kurtosis Normality (Reach=R05, D ate=Dec-06) Omnibus Normality (Reach=R05, Date=Dec-06) Variance-Ratio Equal-Variance Test 1.4666 Modified-Levene Equal-Variance Test 0.4310 Probability 1.0731 -0.5109 1.4125 1.0392 -1.0975 2.2845 0.577451 0.519785 Decision(.050) 0.283239 Cannot reject normality 0.609424 Cannot reject normality 0.493493 Cannot reject normality 0.298698 Cannot reject normality 0.272432 Cannot reject normality 0.319107 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Dec-06 Count 10 10 Median 3355 1127.5 95.0% LCL of Median 720 300 95.0% UCL of Median 8960 5310 4/10/2013 10:40:04 AM 947 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Dec-06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 60 115 40 95 Multiplicity Factor = 0 Mean of W 105 105 Std Dev of W 13.22876 13.22876 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.481251 No 0.759375 No 0.240626 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.7559 0.449692 No 0.7559 0.775154 No 0.7559 0.224846 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.7181 0.472676 No 0.7937 0.786322 No 0.7181 0.236338 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.300000 0.100000 0.300000 Reject H0 if Greater Than 0.5623 0.5623 0.5623 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.7869 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Normal ProbabilityPlotof Largernouth_Bass_PFOS_ppbwhen Reach=R05, Date=Nov 4/10/2013 10:40:04 AM 948 10000-, Largemouth_Bass_PFOS_ppbvs Groups 4/10/2013 10:40:04 AM 949 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Nov-04 10 3897.8 3255.094 1029.351 1569.246 6226.354 Reach=R05, Date=Jul-07 12 1281.408 1086.755 313.7191 590.9171 Note: T-alpha (Reach=R05, Date=Nov-04) = 2.2622, T-alpha (Reach=R05, Date=Jul-07) = 2.2010 1971.9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 2616.392 2327.575 Unequal 10.67 2616.392 3431.716 Note: T-alpha (Equal) = 2.0860, T-alpha (Unequal) = 2.2092 Standard Error 996.6083 1076.096 95.0% LCL Difference 537.5032 239.0712 95.0% UCL Difference 4695.28 4993.712 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.6253 0.016214 Yes Difference < 0 2.6253 0.991893 No Difference > 0 2.6253 0.008107 Yes Difference: (Reach=R05, Date= Nov-04)-(Reach =R05, Date= Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.704616 0.000014 0.813269 Power (Alpha=.010) 0.433751 0.000001 0.548142 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.4314 0.033947 Yes Difference < 0 2.4314 0.983026 No Difference > 0 2.4314 0.016974 Yes Difference: (Reach=R05, Date=Nov-04)-(Reach=R05, Date=Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.598666 0.000041 0.735092 Power (Alpha=.010) 0.304413 0.000003 0.420649 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Nov-04) Kurtosis Normality (Reach=R05, Date=Nov-04) Omnibus Normality (Reach=R05, Date=Nov-04) Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Variance-Ratio Equal-Variance Test 8.9715 Modified-Levene Equal-Variance Test 11.5265 Probability 1.0731 -0.5109 1.4125 1.2669 0.0239 1.6055 0.001281 0.002874 Decision(.050) 0.283239 Cannot reject normality 0.609424 Cannot reject normality 0.493493 Cannot reject normality 0.205206 Cannot reject normality 0.980959 Cannot reject normality 0.448095 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Jul-07 Count 10 12 Median 3355 1090.65 95.0% LCL of Median 720 310.4 95.0% UCL of Median 8960 2044 4/10/2013 10:40:04 AM 950 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Jul-07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 93 148 27 105 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 15.16575 15.16575 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.029961 Yes 0.985020 No 0.014980 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 2.1760 0.029559 Yes 2.1760 0.985221 No 2.1760 0.014779 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.1430 0.032114 Yes 2.2089 0.986410 No 2.1430 0.016057 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.500000 0.000000 0.500000 Reject H0 if Greater Than 0.5408 0.5408 0.5408 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.0927 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Normal ProbabilityPlotof Largernouth_Bass_PFOS_ppbwhen Reach=R05, Date=Nov 4/10/2013 10:40:04 AM 951 10000-, Largemouth_Bass_PFOS_ppbvs Groups 4/10/2013 10:40:05 AM 952 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05, Date=Nov-04 10 3897.8 3255.094 6226.354 Reach=R05, Date=Jul-08 12 1453.35 1467.149 2385.532 Note: T-alpha (Reach=R05, Date=Nov-04) = 2.2622, T-alpha (Reach=R05 95.0% LCL of Mean 1029.351 95.0% UCL of Mean 1569.246 423.5294 521.1681 Date=Jul-08) = 2.2010 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 2444.45 2439.657 Unequal 12.02 2444.45 3570.457 Note: T-alpha (Equal) = 2.0860, T-alpha (Unequal) = 2.1783 Standard Error 1044.599 1113.077 95.0% LCL Difference 265.4551 19.7837 95.0% UCL Difference 4623.445 4869.116 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3401 0.029761 Yes Difference < 0 2.3401 0.985120 No Difference > 0 2.3401 0.014880 Yes Difference: (Reach= R05, Date=Nov-04)-(Reach R05, Date= Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.605226 0.000046 0.730697 Power (Alpha=.010) 0.334509 0.000003 0.442707 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1961 0.048432 Yes Difference < 0 2.1961 0.975784 No Difference > 0 2.1961 0.024216 Yes Difference: (Reach=R05, Date=Nov-04)-(Reach=R05, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.523791 0.000097 0.664696 Power (Alpha=.010) 0.250258 0.000008 0.354182 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Nov-04) Kurtosis Normality (Reach=R05, Date=Nov-04) Omnibus Normality (Reach=R05, Date=Nov-04) Skewness Normality (Reach=R05, Date=Jul-08) Kurtosis Normality (Reach=R05, Date=Jul-08) Omnibus Normality (Reach=R05, Date=Jul-08) Variance-Ratio Equal-Variance Test 4.9224 Modified-Levene Equal-Variance Test 7.0766 Probability 1.0731 -0.5109 1.4125 2.4107 1.9157 9.4811 0.015860 0.015029 Decision(.050) 0.283239 Cannot reject normality 0.609424 Cannot reject normality 0.493493 Cannot reject normality 0.015924 Reject normality 0.055405 Cannot reject normality 0.008734 Reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Jul-08 Count 10 12 Median 3355 929.66 95.0% LCL of Median 720 337.38 95.0% UCL of Median 8960 2253.98 4/10/2013 10:40:05 AM 953 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Jul-08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 91 146 29 107 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 15.16575 15.16575 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.042570 Yes 0.978715 No 0.021285 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 2.0441 0.040946 Yes 2.0441 0.979527 No 2.0441 0.020473 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.0111 0.044314 Yes 2.0770 0.981101 No 2.0111 0.022157 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.416667 0.000000 0.416667 Reject H0 if Greater Than 0.5408 0.5408 0.5408 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.2337 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 05Normal Probability Plot of Largernouth_Bass_PFO S_ppb when R e a c h = R , Date=Nov 4/10/2013 10:40:05 AM 954 10000-, Largemouth_Bass_PFOS_ppb vs Groups 4/10/2013 10:40:05 AM 955 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05, Date=Nov-04 10 3897.8 3255.094 6226.354 Reach=R05, Date=Jul-09 12 1011.468 1023.998 1662.085 Note: T-alpha (Reach=R05, Date=Nov-04) = 2.2622, T-alpha (Reach=R05 95.0% LCL of Mean 1029.351 95.0% UCL of Mean 1569.246 295.6027 360.8504 Date=Jul-09) = 2.2010 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 2886.333 2311.872 Unequal 10.49 2886.333 3412.362 Note: T-alpha (Equal) = 2.0860, T-alpha (Unequal) = 2.2142 Standard Error 989.8845 1070.955 95.0% LCL Difference 821.4697 515.0399 95.0% UCL Difference 4951.195 5257.625 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9158 0.008546 Yes Difference < 0 2.9158 0.995727 No Difference > 0 2.9158 0.004273 Yes Difference: (Reach= R05, Date=Nov-04)-(Reach R05, Date= Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.792033 0.000004 0.879029 Power (Alpha=.010) 0.539208 0.000000 0.652026 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.6951 0.021646 Yes Difference < 0 2.6951 0.989177 No Difference > 0 2.6951 0.010823 Yes Difference: (Reach=R05, Date=Nov-04)-(Reach=R05, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.685583 0.000014 0.807993 Power (Alpha=.010) 0.382729 0.000001 0.508664 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Nov-04) Kurtosis Normality (Reach=R05, Date=Nov-04) Omnibus Normality (Reach=R05, Date=Nov-04) Skewness Normality (Reach=R05, Date=Jul-09) Kurtosis Normality (Reach=R05, Date=Jul-09) Omnibus Normality (Reach=R05, Date=Jul-09) Variance-Ratio Equal-Variance Test 10.1048 Modified-Levene Equal-Variance Test 12.6561 Probability 1.0731 -0.5109 1.4125 2.0222 0.6651 4.5318 0.000746 0.001974 Decision(.050) 0.283239 Cannot reject normality 0.609424 Cannot reject normality 0.493493 Cannot reject normality 0.043152 Reject normality 0.505989 Cannot reject normality 0.103737 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Jul-09 Count 10 12 Median 3355 680.81 95.0% LCL of Median 720 230.63 95.0% UCL of Median 8960 1987.94 4/10/2013 10:40:05 AM 956 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 99 154 21 99 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 15.16575 15.16575 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.008957 Yes 0.995522 No 0.004478 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.5716 0.010123 Yes 2.5716 0.994938 No 2.5716 0.005062 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5386 0.011129 Yes 2.6046 0.995400 No 2.5386 0.005565 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.500000 0.000000 0.500000 Reject H0 if Greater Than 0.5408 0.5408 0.5408 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.0927 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 05Normal Probability Plot of Largernouth_Bass_PFO S_ppb when R e a c h = R , Date=Nov 4/10/2013 10:40:05 AM 957 10000-, Largemouth_Bass_PFOS_ppb vs Groups 4/10/2013 10:40:05 AM 958 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Nov-04 10 3897.8 3255.094 1029.351 1569.246 6226.354 Reach=R05, Date=Apr-12 26 1516.2 1450.952 284.5552 930.1476 2102.252 Note: T-alpha (Reach:=R05, Date=Nov-04) = 2.2622, T-alpha (Reach=R05, Date=Apr-12) = 2.0595 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 34 2381.6 2086.316 Unequal 10.41 2381.6 3563.833 Note: T-alpha (Equal) = 2.0322, T-alpha (Unequal) = 2.2164 Standard Error 776.327 1067.959 95.0% LCL Difference 803.9136 14.57154 95.0% UCL Difference 3959.286 4748.628 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.0678 0.004214 Yes Difference < 0 3.0678 0.997893 No Difference > 0 3.0678 0.002107 Yes Difference: (Reach=R05, D ate=N ov-04)-(R each:=R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.846163 0.000002 0.913241 Power (Alpha=.010) 0.633773 0.000000 0.731868 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2300 0.048842 Yes Difference < 0 2.2300 0.975579 No Difference > 0 2.2300 0.024421 Yes Difference: (Reach=R05, Date=Nov-04)-(Reach=R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.525067 0.000091 0.668938 Power (Alpha=.010) 0.245527 0.000008 0.351410 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Nov-04) Kurtosis Normality (Reach=R05, Date=Nov-04) Omnibus Normality (Reach=R05, Date=Nov-04) Skewness Normality (Reach=R05, Date=Apr-12) Kurtosis Normality (Reach=R05, Date=Apr-12) Omnibus Normality (Reach=R05, Date=Apr-12) Variance-Ratio Equal-Variance Test 5.0329 Modified-Levene Equal-Variance Test 10.3643 Probability 1.0731 -0.5109 1.4125 2.3380 0.4085 5.6331 0.001276 0.002825 Decision(.050) 0.283239 Cannot reject normality 0.609424 Cannot reject normality 0.493493 Cannot reject normality 0.019388 Reject normality 0.682909 Cannot reject normality 0.059813 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Apr-12 Count 10 26 Median 3355 852 95.0% LCL of Median 720 526 95.0% UCL of Median 8960 2020 4/10/2013 10:40:06 AM 959 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Apr-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 197 252 63 414 Multiplicity Factor = 0 Mean of W 185 481 Std Dev of W 28.31372 28.31372 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000000 Yes 0.000000 Yes 0.000000 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.3663 0.017965 Yes 2.3663 0.991018 No 2.3663 0.008982 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.3487 0.018840 Yes 2.3840 0.991437 No 2.3487 0.009420 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.423077 0.000000 0.423077 Reject H0 if Greater Than 0.4744 0.4744 0.4744 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.1117 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 05Normal Probability Plot of Largernouth_Bass_PFO S_ppb when R e a c h = R , Date=Nov 4/10/2013 10:40:06 AM 960 10000-, Largemouth_Bass_PFOS_ppb vs Groups 4/10/2013 10:40:06 AM 961 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Nov-04 10 3897.8 3255.094 1029.351 1569.246 6226.354 Reach=R05, Date=Nov-12 5 404.88 192.5162 86.09584 165.8396 643.9204 Note: T-alpha (Reach=R05, Date=Nov-04) = 2.2622, T-alpha (Reach=R05, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 3492.92 2710.507 Unequal 9.13 3492.92 3260.782 Note: T-alpha (Equal) = 2.1604, T-alpha (Unequal) = 2.2574 Standard Error 1484.606 1032.946 95.0% LCL Difference 285.6245 1161.119 95.0% UCL Difference 6700.215 5824.721 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3528 0.035045 Yes Difference < 0 2.3528 0.982477 No Difference > 0 2.3528 0.017523 Yes II ZJ o en D CD CilD Difference: (Reach= R05, Date=Nov-04)-(Reach :N o v - 1 2 ) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.585997 0.000051 0.720005 Power (Alpha=.010) 0.302734 0.000004 0.414264 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.3815 0.007949 Yes Difference < 0 3.3815 0.996026 No Difference > 0 3.3815 0.003974 Yes Difference: (Reach=R05, Date=Nov-04)-(Reach=R05, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.853267 0.000001 0.929451 Power (Alpha=.010) 0.576568 0.000000 0.707088 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Nov-04) Kurtosis Normality (Reach=R05, Date=Nov-04) Omnibus Normality (Reach=R05, Date=Nov-04) Skewness Normality (Reach=R05, Date=Nov-12) Kurtosis Normality (Reach=R05, Date=Nov-12) Omnibus Normality (Reach=R05, Date=Nov-12) Variance-Ratio Equal-Variance Test 285.8859 Modified-Levene Equal-Variance Test 10.5582 Probability 1.0731 -0.5109 1.4125 0.0000 Decision(.050) 0.283239 Cannot reject normality 0.609424 Cannot reject normality 0.493493 Cannot reject normality 1.000000 Cannot reject normality 0.000059 0.006336 Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Nov-12 Count 10 5 Median 3355 435 95.0% LCL of Median 720 95.0% UCL of Median 8960 4/10/2013 10:40:06 AM 962 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 50 105 0 15 Multiplicity Factor = 0 Mean of W 80 40 Std Dev of W 8.164966 8.164966 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000666 Yes 0.999667 No 0.000333 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.0619 0.002200 Yes -3.0619 0.998900 No -3.0619 0.001100 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.0006 0.002694 Yes -3.1231 0.999105 No -3.0006 0.001347 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.6698 0.6698 0.6698 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0007 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:06 AM 963 4/10/2013 10:40:14 AM 1024 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Dec-06 10 2726 2687.829 849.9661 803.2431 4648.757 Reach=R05, Date=Jul-07 12 1281.408 1086.755 313.7191 590.9171 Note: T-alpha (Reach=R05, Date=D ec-06) = 2.2622, T-alpha (Reach=R05, Date=Jul-07) = 2.2010 1971.9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 1444.592 1974.984 Unequal 11.45 1444.592 2899.217 Note: T-alpha (Equal) = 2.0860, T-alpha (Unequal) = 2.1906 Standard Error 845.6375 906.0144 95.0% LCL Difference -319.3773 -540.1052 95.0% UCL Difference 3208.561 3429.289 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.7083 0.103058 No Difference < 0 1.7083 0.948471 No Difference > 0 1.7083 0.051529 No Difference: (Reach=R05, Date= Dec-06)-(Reach =R05, Date= Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.369523 0.000487 0.502113 Power (Alpha=.010) 0.157265 0.000043 0.232084 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.5944 0.138059 No Difference < 0 1.5944 0.930970 No Difference > 0 1.5944 0.069030 No Difference: (Reach=R05, Date=Dec-06)-(Reach=R05, Date=Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.309511 0.000813 0.442262 Power (Alpha=.010) 0.114924 0.000086 0.180257 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Dec-06) Kurtosis Normality (Reach=R05, D ate=Dec-06) Omnibus Normality (Reach=R05, Date=Dec-06) Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Variance-Ratio Equal-Variance Test 6.1170 Modified-Levene Equal-Variance Test 3.1170 Probability 1.0392 -1.0975 2.2845 1.2669 0.0239 1.6055 0.006677 0.092743 Decision(.050) 0.298698 Cannot reject normality 0.272432 Cannot reject normality 0.319107 Cannot reject normality 0.205206 Cannot reject normality 0.980959 Cannot reject normality 0.448095 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-07 Count 10 12 Median 1127.5 1090.65 95.0% LCL of Median 300 310.4 95.0% UCL of Median 5310 2044 4/10/2013 10:40:14 AM 1025 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 75 130 45 123 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 15.16575 15.16575 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.346279 No 0.826860 No 0.173140 No Approx. Without Correction Prob Z-Value Level Reject H0 at .050 0.9891 0.322629 No 0.9891 0.838686 No 0.9891 0.161314 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.9561 0.339021 No 1.0220 0.846619 No 0.9561 0.169510 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.033333 0.400000 Reject H0 if Greater Than 0.5408 0.5408 0.5408 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.2829 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 05Normal Probability Plot of Largem outh_Bass_PFOS_ppb when R e a c h = R , Date=De 4/10/2013 10:40:14 AM 1026 3000n Largemouth_Bass_PFOS_ppb vs Groups 6000- Cl. Reach=R05,Date=Dec-06 Reach--R05,Date=JuI-07 Groups 4/10/2013 10:40:14 AM 1027 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Dec-06 10 2726 2687.829 849.9661 803.2431 4648.757 Reach=R05, Date=Jul-08 12 1453.35 1467.149 423.5294 521.1681 2385.532 Note: T-alpha (Reach=R05, Date=D ec-06) = 2.2622, T-alpha (Reach=R05, Date=Jul-08) = 2.2010 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 1272.65 2105.916 Unequal 13.35 1272.65 3062.18 Note: T-alpha (Equal) = 2.0860, T-alpha (Unequal) = 2.1546 Standard Error 901.6991 949.6418 95.0% LCL Difference -608.2614 -773.4616 95.0% UCL Difference 3153.561 3318.761 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1 .41 14 0.173497 No Difference < 0 1.41 14 0.913251 No Difference > 0 1.41 14 0.086749 No Difference: (Reach=R05, Date= Dec-06)-(Reach =R05, Date= Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.269450 0.001305 0.389239 Power (Alpha=.010) 0.100672 0.000132 0.156585 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1 .3401 0.202564 No Difference < 0 1.3401 0.898718 No Difference > 0 1.3401 0.101282 No Difference: (Reach=R05, Date=Dec-06)-(Reach=R05, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.237968 0.001747 0.354694 Power (Alpha=.010) 0.081720 0.000197 0.131872 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Dec-06) Kurtosis Normality (Reach=R05, D ate=Dec-06) Omnibus Normality (Reach=R05, Date=Dec-06) Skewness Normality (Reach=R05, Date=Jul-08) Kurtosis Normality (Reach=R05, Date=Jul-08) Omnibus Normality (Reach=R05, Date=Jul-08) Variance-Ratio Equal-Variance Test 3.3563 Modified-Levene Equal-Variance Test 1.8507 Probability 1.0392 -1.0975 2.2845 2.4107 1.9157 9.4811 0.062536 0.188839 Decision(.050) 0.298698 Cannot reject normality 0.272432 Cannot reject normality 0.319107 Cannot reject normality 0.015924 Reject normality 0.055405 Cannot reject normality 0.008734 Reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-08 Count 10 12 Median 1127.5 929.66 95.0% LCL of Median 300 337.38 95.0% UCL of Median 5310 2253.98 4/10/2013 10:40:14 AM 1028 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 73 128 47 125 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 15.16575 15.16575 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.417582 No 0.791209 No 0.208791 No Approx. Without Correction Prob Z-Value Level Reject H0 at .050 0.8572 0.391337 No 0.8572 0.804331 No 0.8572 0.195669 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.8242 0.409811 No 0.8902 0.813311 No 0.8242 0.204906 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.316667 0.100000 0.316667 Reject H0 if Greater Than 0.5408 0.5408 0.5408 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.5577 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 05Normal Probability Plot of Largem outh_Bass_PFOS_ppb when R e a c h = R , Date=De 4/10/2013 10:40:14 AM 1029 3000n Largemouth_Bass_PFOS_ppb vs Groups 6000- Q_ 0 J---------------------------- 1--------------------------------------------------------- 1----------------- Reach=R05,Date=Dec-06 Reach--R05,Date=JuI-03 Groups 4/10/2013 10:40:14 AM 1030 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Dec-06 10 2726 2687.829 849.9661 803.2431 4648.757 Reach=R05, Date=Jul-09 12 1011.468 1023.998 295.6027 360.8504 1662.085 Note: T-alpha (Reach=R05, Date=D ec-06) = 2.2622, T-alpha (Reach=R05, Date=Jul-09) = 2.2010 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 1714.532 1956.452 Unequal 11.18 1714.532 2876.281 Note: T-alpha (Equal) = 2.0860, T-alpha (Unequal) = 2.1968 Standard Error 837.7028 899.9019 95.0% LCL Difference -32.88482 -262.3594 95.0% UCL Difference 3461.95 3691.425 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0467 0.054056 No Difference < 0 2.0467 0.972972 No Difference > 0 2.0467 0.027028 Yes Difference: (Reach=R05, Date= Dec-06)-(Reach =R05, Date= Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.495310 0.000144 0.630013 Power (Alpha=.010) 0.243252 0.000011 0.338346 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.9052 0.082791 No Difference < 0 1.9052 0.958605 No Difference > 0 1.9052 0.041395 Yes Difference: (Reach=R05, Date=Dec-06)-(Reach=R05, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.413719 0.000287 0.557047 Power (Alpha=.010) 0.173221 0.000028 0.259041 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Dec-06) Kurtosis Normality (Reach=R05, D ate=Dec-06) Omnibus Normality (Reach=R05, Date=Dec-06) Skewness Normality (Reach=R05, Date=Jul-09) Kurtosis Normality (Reach=R05, Date=Jul-09) Omnibus Normality (Reach=R05, Date=Jul-09) Variance-Ratio Equal-Variance Test 6.8898 Modified-Levene Equal-Variance Test 3.8146 Probability 1.0392 -1.0975 2.2845 2.0222 0.6651 4.5318 0.004062 0.064943 Decision(.050) 0.298698 Cannot reject normality 0.272432 Cannot reject normality 0.319107 Cannot reject normality 0.043152 Reject normality 0.505989 Cannot reject normality 0.103737 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-09 Count 10 12 Median 1127.5 680.81 95.0% LCL of Median 300 230.63 95.0% UCL of Median 5310 1987.94 4/10/2013 10:40:14 AM 1031 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 87 142 33 111 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 15.16575 15.16575 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.080421 No 0.959789 No 0.040211 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 1.7803 0.075022 No 1.7803 0.962489 No 1.7803 0.037511 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.7474 0.080575 No 1.8133 0.965107 No 1.7474 0.040288 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.450000 0.000000 0.450000 Reject H0 if Greater Than 0.5408 0.5408 0.5408 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.1598 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 05Normal Probability Plot of Largem outh_Bass_PFOS_ppb when R e a c h = R , Date=De 4/10/2013 10:40:15 AM 1032 3000n Largemouth_Bass_PFOS_ppb vs Groups 6000- Cl. Reach=R05,Date=Dec-06 Reach--R05,Date=JuI-09 Groups 4/10/2013 10:40:15 AM 1033 Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Dec-06 10 2726 2687.829 849.9661 803.2431 4648.757 Reach=R05, Date=Apr-12 26 1516.2 1450.952 284.5552 930.1476 2102.252 Note: T-alpha (Reach:=R05, Date=D ec-06) = 2.2622, T-alpha (Reach=R05, Date=Apr-12) = 2.0595 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 34 1209.8 1860.198 Unequal 11.08 1209.8 3054.454 Note: T-alpha (Equal) = 2.0322, T-alpha (Unequal) = 2.1990 Standard Error 692.1873 896.3337 95.0% LCL Difference -196.8939 -761.272 95.0% UCL Difference 2616.494 3180.872 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1 .7478 0.089527 No Difference < 0 1.7478 0.955237 No Difference > 0 1.7478 0.044763 Yes Difference: (Reach=R05, Date= Dec-06)-(Reach =R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.396968 0.000392 0.527114 Power (Alpha=.010) 0.180837 0.000031 0.258617 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1 .3497 0.204035 No Difference < 0 1.3497 0.897983 No Difference > 0 1.3497 0.102017 No Difference: (Reach=R05, Date=Dec-06)-(Reach=R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.234804 0.001765 0.352872 Power (Alpha=.010) 0.078452 0.000207 0.128234 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Dec-06) Kurtosis Normality (Reach=R05, D ate=Dec-06) Omnibus Normality (Reach=R05, Date=Dec-06) Skewness Normality (Reach=R05, Date=Apr-12) Kurtosis Normality (Reach=R05, Date=Apr-12) Omnibus Normality (Reach=R05, Date=Apr-12) Variance-Ratio Equal-Variance Test 3.4316 Modified-Levene Equal-Variance Test 3.0257 Probability 1.0392 -1.0975 2.2845 2.3380 0.4085 5.6331 0.014077 0.091004 Decision(.050) 0.298698 Cannot reject normality 0.272432 Cannot reject normality 0.319107 Cannot reject normality 0.019388 Reject normality 0.682909 Cannot reject normality 0.059813 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Apr-12 Count 10 26 Median 1127.5 852 95.0% LCL of Median 300 526 95.0% UCL of Median 5310 2020 4/10/2013 10:40:15 AM 1034 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Apr-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 162 21 7 98 449 Multiplicity Factor = 0 Mean of W 185 481 Std Dev of W 28.31372 28.31372 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000000 Yes 0.000000 Yes 0.000000 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.1302 0.258394 No 1.1302 0.870803 No 1.1302 0.129197 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.1125 0.265908 No 1.1479 0.874485 No 1.1 125 0.132954 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.061538 0.400000 Reject H0 if Greater Than 0.4744 0.4744 0.4744 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.1492 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:15 AM Normal Probability Plot of Largemouth_Bass_PFOS_ppb when Reach=R05, Date=De 1035 3000 n Largemouth_Bass_PFOS_ppb vs Groups 6000 - Cl. Reach=R05, Date=Dec-0B Reach--R05, Date=A.pr-12 Groups 4/10/2013 10:40:15 AM 1036 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Dec-06 10 2726 2687.829 849.9661 803.2431 4648.757 Reach=R05, Date=Nov-12 5 404.88 192.5162 86.09584 165.8396 643.9204 Note: T-alpha (R e ac h =R05, Date=D ec-06) = 2.2622, T-alpha (Reach=R05, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 2321.12 2238.957 Unequal 9.18 2321.12 2694.715 Note: T-alpha (Equal) = 2.1604, T-alpha (Unequal) = 2.2553 Standard Error 1226.327 854.3154 95.0% LCL Difference -328.1989 394.3934 95.0% UCL Difference 4970.439 4247.847 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.8927 0.080869 No Difference < 0 1.8927 0.959565 No Difference > 0 1.8927 0.040435 Yes II ZJ o en D CD ilCD Difference: (Reach=R05, Date= Dec-06)-(Reach :N o v - 1 2 ) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.418108 0.000285 0.558911 Power (Alpha=.010) 0.179795 0.000026 0.265406 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7169 0.023312 Yes Difference < 0 2.7169 0.988344 No Difference > 0 2.7169 0.011656 Yes Difference: (Reach=R05, Date=Dec-06)-(Reach=R05, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.679626 0.000014 0.806295 Power (Alpha=.010) 0.368063 0.000001 0.496802 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Dec-06) Kurtosis Normality (Reach=R05, D ate=Dec-06) Omnibus Normality (Reach=R05, Date=Dec-06) Skewness Normality (Reach=R05, Date=Nov-12) Kurtosis Normality (Reach=R05, Date=Nov-12) Omnibus Normality (Reach=R05, Date=Nov-12) Variance-Ratio Equal-Variance Test 194.9256 Modified-Levene Equal-Variance Test 3.5042 Probability 1.0392 -1.0975 2.2845 0.0000 Decision(.050) 0.298698 Cannot reject normality 0.272432 Cannot reject normality 0.319107 Cannot reject normality 1.000000 Cannot reject normality 0.000127 0.083880 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Nov-12 Count 10 5 Median 1127.5 435 95.0% LCL of Median 300 95.0% UCL of Median 5310 4/10/2013 10:40:15 AM 1037 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 42 97 8 23 Multiplicity Factor = 0 Mean of W 80 40 Std Dev of W 8.164966 8.164966 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.039960 Yes 0.980020 No 0.019980 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 -2.0821 0.037336 Yes -2.0821 0.981332 No -2.0821 0.018668 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.0208 0.043297 Yes -2.1433 0.983956 No -2.0208 0.021649 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.6698 0.6698 0.6698 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0193 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:15 AM 1038 4/10/2013 10:40:24 AM 1099 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-07 12 1281.408 1086.755 313.7191 590.9171 Reach=R05, Date=Jul-08 12 1453.35 1467.149 423.5294 521.1681 2385.532 Note: T-alpha (Reach=R05, Date=Jul-07) = 2.2010, T-alpha (Reach=R05, Date=Jul-08) = 2.2010 1971.9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 22 -171.9417 1291.039 Unequal 20.28 -171.9417 1825.805 Note: T-alpha (Equal) = 2.0739, T-alpha (Unequal) = 2.0841 Standard Error 527.0644 527.0644 95.0% LCL Difference -1265.006 -1270.414 95.0% UCL Difference 921.1229 926.5305 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.3262 0.747337 No Difference < 0 -0.3262 0.373668 No Difference > 0 -0.3262 0.626332 No Difference: (Reach=R05, Date=Jul-07)-(Reach= R05, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.061241 0.091999 0.024926 Power (Alpha=.010) 0.013506 0.021718 0.004226 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.3262 0.747597 No Difference < 0 -0.3262 0.373798 No Difference > 0 -0.3262 0.626202 No Difference: (Reach=R05, Date=Jul-07)-(Reach=R05, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.061157 0.091862 0.024973 Power (Alpha=.010) 0.013461 0.021637 0.004246 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Skewness Normality (Reach=R05, Date=Jul-08) Kurtosis Normality (Reach=R05, Date=Jul-08) Omnibus Normality (Reach=R05, Date=Jul-08) Variance-Ratio Equal-Variance Test 1.8226 Modified-Levene Equal-Variance Test 0.3053 Probability 1.2669 0.0239 1.6055 2.4107 1.9157 9.4811 0.334008 0.586178 Decision(.050) 0.205206 Cannot reject normality 0.980959 Cannot reject normality 0.448095 Cannot reject normality 0.015924 Reject normality 0.055405 Cannot reject normality 0.008734 Reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Jul-08 Count 12 12 Median 1090.65 929.66 95.0% LCL of Median 310.4 337.38 95.0% UCL of Median 2044 2253.98 4/10/2013 10:40:24 AM 1100 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Jul-08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 66 144 78 156 Multiplicity Factor = 0 Mean of W 150 150 Std Dev of W 17.32051 17.32051 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.755285 No 0.377642 No 0.622358 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.3464 0.729034 No -0.3464 0.364517 No -0.3464 0.635483 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.3175 0.750832 No -0.3175 0.375416 No -0.3753 0.646273 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.166667 0.166667 0.083333 Reject H0 if Greater Than 0.5179 0.5179 0.5179 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.9985 Plots Section 5 -||---------------------- Histogram 40 - I 30 n I| 20- 10- ------------- Largemojth_Bass_FFOS_ppb when Reach=R05, Date=Jul-07 o -4------------------- ----------------------------------------------------------- 1--------------------------------------- 0 1000 1000 3000 4300 5000 6000 Largemouth_Bass_RFOS_piob when Reach=R05, Date=Jul-oe Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:24 AM 1101 4/10/2013 10:40:24 AM 1102 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-07 12 1281.408 1086.755 313.7191 590.9171 Reach=R05, Date=Jul-09 12 1011.468 1023.998 295.6027 360.8504 1662.085 Note: T-alpha (Reach=R05, Date=Jul-07) = 2.2010, T-alpha (Reach=R05, Date=Jul-09) = 2.2010 1971.9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 22 269.9408 1055.843 Unequal 21.92 269.9408 1493.187 Note: T-alpha (Equal) = 2.0739, T-alpha (Unequal) = 2.0743 Standard Error 431.046 431.046 95.0% LCL Difference -623.9938 -624.1768 95.0% UCL Difference 1163.875 1164.058 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6262 0.537598 No Difference < 0 0.6262 0.731201 No Difference > 0 0.6262 0.268799 No Difference: (Reach=R05, Date= Jul-07)-(Reach= R05, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.092057 0.012152 0.149701 Power (Alpha=.010) 0.023822 0.001773 0.041153 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6262 0.537620 No Difference < 0 0.6262 0.731190 No Difference > 0 0.6262 0.268810 No Difference: (Reach=R05, Date=Jul-07)-(Reach=R05, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.092044 0.012154 0.149685 Power (Alpha=.010) 0.023814 0.001773 0.041142 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Skewness Normality (Reach=R05, Date=Jul-09) Kurtosis Normality (Reach=R05, Date=Jul-09) Omnibus Normality (Reach=R05, Date=Jul-09) Variance-Ratio Equal-Variance Test 1.1263 Modified-Levene Equal-Variance Test 0.3069 Probability 1.2669 0.0239 1.6055 2.0222 0.6651 4.5318 0.847118 0.585159 Decision(.050) 0.205206 Cannot reject normality 0.980959 Cannot reject normality 0.448095 Cannot reject normality 0.043152 Reject normality 0.505989 Cannot reject normality 0.103737 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Jul-09 Count 12 12 Median 1090.65 680.81 95.0% LCL of Median 310.4 230.63 95.0% UCL of Median 2044 1987.94 4/10/2013 10:40:24 AM 1103 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 82 160 62 140 Multiplicity Factor = 0 Mean of W 150 150 Std Dev of W 17.32051 17.32051 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.589876 No 0.705062 No 0.294938 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.5774 0.563703 No 0.5774 0.718149 No 0.5774 0.281851 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.5485 0.583360 No 0.6062 0.727815 No 0.5485 0.291680 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.333333 0.166667 0.333333 Reject H0 if Greater Than 0.5179 0.5179 0.5179 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.5361 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:25 AM Normal Probability Plot of Largernouth_Bass_PFOS_ppb when Reach=R05, Date=Jul 1104 4000 n Langemouth_Bass_PFOS_ppb vs Groups 4/10/2013 10:40:25 AM 1105 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-07 12 1281.408 1086.755 313.7191 590.9171 Reach=R05, Date=Apr-12 26 1516.2 1450.952 284.5552 930.1476 2102.252 Note: T-alpha (Reach=R05, Date=Jul-07) = 2.2010, T-alpha (Reach=R05, Date=Apr-12) = 2.0595 1971.9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 36 -234.7917 1350.133 Unequal 28.16 -234.7917 1812.815 Note: T-alpha (Equal) = 2.0281, T-alpha (Unequal) = 2.0479 Standard Error 471.1847 423.5462 95.0% LCL Difference -1190.399 -1102.166 95.0% UCL Difference 720.8152 632.5827 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.4983 0.621303 No Difference < 0 -0.4983 0.310651 No Difference > 0 -0.4983 0.689349 No Difference: (Reach=R05, Date=Jul-07)-(Reach= R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.077370 0.123868 0.016425 Power (Alpha=.010) 0.019006 0.032416 0.002504 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.5543 0.583717 No Difference < 0 -0.5543 0.291859 No Difference > 0 -0.5543 0.708141 No Difference: (Reach=R05, Date=Jul-07)-(Reach=R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.083467 0.134853 0.014404 Power (Alpha=.010) 0.021003 0.036089 0.002151 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Skewness Normality (Reach=R05, Date=Apr-12) Kurtosis Normality (Reach=R05, Date=Apr-12) Omnibus Normality (Reach=R05, Date=Apr-12) Variance-Ratio Equal-Variance Test 1.7826 Modified-Levene Equal-Variance Test 0.3698 Probability 1.2669 0.0239 1.6055 2.3380 0.4085 5.6331 0.317013 0.546921 Decision(.050) 0.205206 Cannot reject normality 0.980959 Cannot reject normality 0.448095 Cannot reject normality 0.019388 Reject normality 0.682909 Cannot reject normality 0.059813 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Apr-12 Count 12 26 Median 1090.65 852 95.0% LCL of Median 310.4 526 95.0% UCL of Median 2044 2020 4/10/2013 10:40:25 AM 1106 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Apr-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 148 226 164 515 Multiplicity Factor = 0 Mean of W 234 507 Std Dev of W 31.84337 31.84337 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000000 Yes 0.000000 Yes 0.000000 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.2512 0.801637 No -0.2512 0.400818 No -0.2512 0.599182 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.2355 0.813799 No -0.2355 0.406900 No -0.2669 0.605239 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.224359 0.224359 0.153846 Reject H0 if Greater Than 0.4468 0.4468 0.4468 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.7189 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:25 AM Normal Probability Plot of Largernouth_Bass_PFOS_ppb when Reach=R05, Date=Jul 1107 5000 n Langemouth_Bass_PFOS_ppb vs Groups 4/10/2013 10:40:25 AM 1108 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-07 12 1281.408 1086.755 313.7191 590.9171 Reach=R05, Date=Nov-12 5 404.88 192.5162 86.09584 165.8396 643.9204 Note: T-alpha (Reach=R05, Date=Jul-07) = 2.2010, T-alpha (Reach=R05, Date=Nov-12) = 2.7764 1971.9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 15 876.5283 935.9363 Unequal 12.52 876.5283 1103.675 Note: T-alpha (Equal) = 2.1314, T-alpha (Unequal) = 2.1687 Standard Error 498.1901 325.3186 95.0% LCL Difference -185.3388 170.995 95.0% UCL Difference 1938.396 1582.062 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.7594 0.098879 No Difference < 0 1.7594 0.950560 No Difference > 0 1.7594 0.049440 Yes Difference: (Reach=R05, Date= Jul-07)-(Reach= R05, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.377325 0.000435 0.513781 Power (Alpha=.010) 0.157542 0.000040 0.234959 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.6944 0.018899 Yes Difference < 0 2.6944 0.990551 No Difference > 0 2.6944 0.009449 Yes Difference: (Reach=R05, Date=Jul-07)-(Reach=R05, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.699859 0.000013 0.815867 Power (Alpha=.010) 0.407788 0.000001 0.530582 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Skewness Normality (Reach=R05, Date=Nov-12) Kurtosis Normality (Reach=R05, Date=Nov-12) Omnibus Normality (Reach=R05, Date=Nov-12) Variance-Ratio Equal-Variance Test 31.8661 Modified-Levene Equal-Variance Test 6.6392 Probability 1.2669 0.0239 1.6055 0.0000 Decision(.050) 0.205206 Cannot reject normality 0.980959 Cannot reject normality 0.448095 Cannot reject normality 1.000000 Cannot reject normality 0.004399 0.021054 Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Nov-12 Count 12 5 Median 1090.65 435 95.0% LCL of Median 310.4 95.0% UCL of Median 2044 4/10/2013 10:40:25 AM 1109 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 40 118 20 35 Multiplicity Factor = 0 Mean of W 108 45 Std Dev of W 9.486833 9.486833 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.328378 No 0.835811 No 0.164189 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.0541 0.291841 No -1.0541 0.854080 No -1.0541 0.145920 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.0014 0.316639 No -1.1068 0.865809 No -1.0014 0.158320 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.583333 0.216667 0.583333 Reject H0 if Greater Than 0.6544 0.6544 0.6544 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.1312 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:25 AM 1110 4/10/2013 10:40:33 AM 1171 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-08 12 1453.35 1467.149 423.5294 521.1681 2385.532 Reach=R05, Date=Jul-09 12 1011.468 1023.998 295.6027 360.8504 1662.085 Note: T-alpha (Reach=R05, Date=Jul-08) = 2.2010, T-alpha (Reach=R05, Date=Jul-09) = 2.2010 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 22 441.8825 1265.128 Unequal 19.66 441.8825 1789.161 Note: T-alpha (Equal) = 2.0739, T-alpha (Unequal) = 2.0883 Standard Error 516.4863 516.4863 95.0% LCL Difference -629.2445 -636.679 95.0% UCL Difference 1513.01 1520.444 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.8556 0.401466 No Difference < 0 0.8556 0.799267 No Difference > 0 0.8556 0.200733 No Difference: (Reach= R05, Date=Jul-08)-(Reach= R05, Date= Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.129605 0.006666 0.207398 Power (Alpha=.010) 0.037713 0.000868 0.064002 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.8556 0.402550 No Difference < 0 0.8556 0.798725 No Difference > 0 0.8556 0.201275 No Difference: (Reach=R05, Date=Jul-08)-(Reach=R05, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.128746 0.006721 0.206514 Power (Alpha=.010) 0.037150 0.000884 0.063266 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-08) Kurtosis Normality (Reach=R05, Date=Jul-08) Omnibus Normality (Reach=R05, Date=Jul-08) Skewness Normality (Reach=R05, Date=Jul-09) Kurtosis Normality (Reach=R05, Date=Jul-09) Omnibus Normality (Reach=R05, Date=Jul-09) Variance-Ratio Equal-Variance Test 2.0528 Modified-Levene Equal-Variance Test 0.8656 Probability 2.4107 1.9157 9.4811 2.0222 0.6651 4.5318 0.248503 0.362260 Decision(.050) 0.015924 Reject normality 0.055405 Cannot reject normality 0.008734 Reject normality 0.043152 Reject normality 0.505989 Cannot reject normality 0.103737 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-08 Reach=R05, Date=Jul-09 Count 12 12 Median 929.66 680.81 95.0% LCL of Median 337.38 230.63 95.0% UCL of Median 2253.98 1987.94 4/10/2013 10:40:33 AM 1172 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-08 Reach=R05, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 83 161 61 139 Multiplicity Factor = 0 Mean of W 150 150 Std Dev of W 17.32051 17.32051 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.551167 No 0.724416 No 0.275584 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.6351 0.525373 No 0.6351 0.737314 No 0.6351 0.262686 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.6062 0.544370 No 0.6640 0.746640 No 0.6062 0.272185 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.250000 0.083333 0.250000 Reject H0 if Greater Than 0.5179 0.5179 0.5179 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8690 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:33 AM 1173 4/10/2013 10:40:33 AM 1174 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-08 12 1453.35 1467.149 423.5294 521.1681 2385.532 Reach=R05, Date=Apr-12 26 1516.2 1450.952 284.5552 930.1476 2102.252 Note: T-alpha (R e ac h =R05, Date=Jul-08) = 2.2010, T-alpha (Reach=R05, Date=Apr-12) = 2.0595 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 36 -62.85 1455.92 Unequal 21.27 -62.85 2063.441 Note: T-alpha (Equal) = 2.0281, T-alpha (Unequal) = 2.0780 Standard Error 508.1035 510.2439 95.0% LCL Difference -1093.332 -1123.153 95.0% UCL Difference 967.6315 997.4535 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.1237 0.902245 No Difference < 0 -0.1237 0.451122 No Difference > 0 -0.1237 0.548878 No Difference: (Reach=R05, Date=Jul-08)-(Reach= R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.051663 0.063821 0.038677 Power (Alpha=.010) 0.010522 0.013650 0.007232 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.1232 0.903124 No Difference < 0 -0.1232 0.451562 No Difference > 0 -0.1232 0.548438 No Difference: (Reach=R05, Date=Jul-08)-(Reach=R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.051589 0.063562 0.038851 Power (Alpha=.010) 0.010486 0.013528 0.007302 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-08) Kurtosis Normality (Reach=R05, Date=Jul-08) Omnibus Normality (Reach=R05, Date=Jul-08) Skewness Normality (Reach=R05, Date=Apr-12) Kurtosis Normality (Reach=R05, Date=Apr-12) Omnibus Normality (Reach=R05, Date=Apr-12) Variance-Ratio Equal-Variance Test 1.0224 Modified-Levene Equal-Variance Test 0.0016 Probability 2.4107 1.9157 9.4811 2.3380 0.4085 5.6331 0.912649 0.968256 Decision(.050) 0.015924 Reject normality 0.055405 Cannot reject normality 0.008734 Reject normality 0.019388 Reject normality 0.682909 Cannot reject normality 0.059813 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-08 Reach=R05, Date=Apr-12 Count 12 26 Median 929.66 852 95.0% LCL of Median 337.38 526 95.0% UCL of Median 2253.98 2020 4/10/2013 10:40:33 AM 1175 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-08 Reach=R05, Date=Apr-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 148 226 164 515 Multiplicity Factor = 0 Mean of W 234 507 Std Dev of W 31.84337 31.84337 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000000 Yes 0.000000 Yes 0.000000 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.2512 0.801637 No -0.2512 0.400818 No -0.2512 0.599182 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.2355 0.813799 No -0.2355 0.406900 No -0.2669 0.605239 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.185897 0.185897 0.083333 Reject H0 if Greater Than 0.4468 0.4468 0.4468 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8822 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:33 AM 1176 4/10/2013 10:40:33 AM 1177 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-08 12 1453.35 1467.149 423.5294 521.1681 2385.532 Reach=R05, Date=Nov-12 5 404.88 192.5162 86.09584 165.8396 643.9204 Note: T-alpha (R e ac h =R05, Date=Jul-08) = 2.2010, T-alpha (Reach=R05, Date =Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 15 1048.47 1260.318 Unequal 11.87 1048.47 1479.726 Note: T-alpha (Equal) = 2.1314, T-alpha (Unequal) = 2.1814 Standard Error 670.8557 432.1917 95.0% LCL Difference -381.4249 105.6793 95.0% UCL Difference 2478.365 1991.261 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1 .5629 0.138928 No Difference < 0 1.5629 0.930536 No Difference > 0 1.5629 0.069464 No Difference: (Reach=R05, Date=Jul-08)-(Reach= R05, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.310074 0.000842 0.439276 Power (Alpha=.010) 0.119160 0.000084 0.183760 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.4259 0.032167 Yes Difference < 0 2.4259 0.983916 No Difference > 0 2.4259 0.016084 Yes Difference: (Reach=R05, Date=Jul-08)-(Reach=R05, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.605403 0.000040 0.738735 Power (Alpha=.010) 0.315317 0.000003 0.430599 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-08) Kurtosis Normality (Reach=R05, Date=Jul-08) Omnibus Normality (Reach=R05, Date=Jul-08) Skewness Normality (Reach=R05, Date=Nov-12) Kurtosis Normality (Reach=R05, Date=Nov-12) Omnibus Normality (Reach=R05, Date=Nov-12) Variance-Ratio Equal-Variance Test 58.0783 Modified-Levene Equal-Variance Test 3.5531 Probability 2.4107 1.9157 9.4811 0.0000 Decision(.050) 0.015924 Reject normality 0.055405 Cannot reject normality 0.008734 Reject normality 1.000000 Cannot reject normality 0.001358 0.078959 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-08 Reach=R05, Date=Nov-12 Count 12 5 Median 929.66 435 95.0% LCL of Median 337.38 95.0% UCL of Median 2253.98 4/10/2013 10:40:34 AM 1178 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-08 Reach=R05, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 43 121 17 32 Multiplicity Factor = 0 Mean of W 108 45 Std Dev of W 9.486833 9.486833 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.194570 No 0.902715 No 0.097285 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.3703 0.170587 No -1.3703 0.914707 No -1.3703 0.085293 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.3176 0.187632 No -1.4230 0.922636 No -1.3176 0.093816 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.583333 0.083333 0.583333 Reject H0 if Greater Than 0.6544 0.6544 0.6544 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.1312 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:34 AM 1179 4/10/2013 10:40:41 AM 1240 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-09 12 1011.468 1023.998 295.6027 360.8504 1662.085 Reach=R05, Date=Apr-12 26 1516.2 1450.952 284.5552 930.1476 2102.252 Note: T-alpha (R e ac h =R05, Date=Jul-09) = 2.2010, T-alpha (Reach=R05, Date=Apr-12) = 2.0595 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 36 -504.7325 1335.06 Unequal 29.64 -504.7325 1775.904 Note: T-alpha (Equal) = 2.0281, T-alpha (Unequal) = 2.0433 Standard Error 465.9242 410.3079 95.0% LCL Difference -1449.671 -1343.126 95.0% UCL Difference 440.2055 333.6609 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.0833 0.285881 No Difference < 0 -1.0833 0.142940 No Difference > 0 -1.0833 0.857060 No Difference: (Reach=R05, Date=Jul-09)-(Reach= R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.183912 0.280318 0.003383 Power (Alpha=.010) 0.061752 0.099707 0.000375 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.2301 0.228324 No Difference < 0 -1.2301 0.114162 No Difference > 0 -1.2301 0.885838 No Difference: (Reach=R05, Date=Jul-09)-(Reach=R05, Date=Apr-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.221621 0.328954 0.002203 Power (Alpha=.010) 0.078911 0.124752 0.000230 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-09) Kurtosis Normality (Reach=R05, Date=Jul-09) Omnibus Normality (Reach=R05, Date=Jul-09) Skewness Normality (Reach=R05, Date=Apr-12) Kurtosis Normality (Reach=R05, Date=Apr-12) Omnibus Normality (Reach=R05, Date=Apr-12) Variance-Ratio Equal-Variance Test 2.0077 Modified-Levene Equal-Variance Test 1.0513 Probability 2.0222 0.6651 4.5318 2.3380 0.4085 5.6331 0.226992 0.312057 Decision(.050) 0.043152 Reject normality 0.505989 Cannot reject normality 0.103737 Cannot reject normality 0.019388 Reject normality 0.682909 Cannot reject normality 0.059813 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-09 Reach=R05, Date=Apr-12 Count 12 26 Median 680.81 852 95.0% LCL of Median 230.63 526 95.0% UCL of Median 1987.94 2020 4/10/2013 10:40:41 AM 1241 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-09 Reach=R05, Date=Apr-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 125 203 187 538 Multiplicity Factor = 0 Mean of W 234 507 Std Dev of W 31.84337 31.84337 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000000 Yes 0.000000 Yes 0.000000 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.9735 0.330297 No -0.9735 0.165149 No -0.9735 0.834851 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.9578 0.338157 No -0.9578 0.169078 No -0.9892 0.838722 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.250000 0.250000 0.076923 Reject H0 if Greater Than 0.4468 0.4468 0.4468 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.5996 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:41 AM 1242 4/10/2013 10:40:41 AM 1243 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-09 12 1011.468 1023.998 295.6027 360.8504 1662.085 Reach=R05, Date=Nov-12 5 404.88 192.5162 86.09584 165.8396 643.9204 Note: T-alpha (Reach=R05, Date=Jul-09) = 2.2010, T-alpha (Reach=R05, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 15 606.5875 882.5166 Unequal 12.69 606.5875 1041.938 Note: T-alpha (Equal) = 2.1314, T-alpha (Unequal) = 2.1657 Standard Error 469.7553 307.8854 95.0% LCL Difference -394.6723 -60.19099 95.0% UCL Difference 1607.847 1273.366 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.2913 0.216151 No Difference < 0 1.2913 0.891925 No Difference > 0 1.2913 0.108075 No Difference: (Reach=R05, Date=Jul-09)-(Reach=R05, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.227270 0.001985 0.340238 Power (Alpha=.010) 0.077736 0.000223 0.125467 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.9702 0.071030 No Difference < 0 1.9702 0.964485 No Difference > 0 1.9702 0.035515 Yes Difference: (Reach=R05, Date=Jul-09)-(Reach=R05, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.444789 0.000218 0.586626 Power (Alpha=.010) 0.196446 0.000020 0.286932 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-09) Kurtosis Normality (Reach=R05, Date=Jul-09) Omnibus Normality (Reach=R05, Date=Jul-09) Skewness Normality (Reach=R05, Date=Nov-12) Kurtosis Normality (Reach=R05, Date=Nov-12) Omnibus Normality (Reach=R05, Date=Nov-12) Variance-Ratio Equal-Variance Test 28.2920 Modified-Levene Equal-Variance Test 2.6060 Probability 2.0222 0.6651 4.5318 0.0000 Decision(.050) 0.043152 Reject normality 0.505989 Cannot reject normality 0.103737 Cannot reject normality 1.000000 Cannot reject normality 0.005541 0.127292 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-09 Reach=R05, Date=Nov-12 Count 12 5 Median 680.81 435 95.0% LCL of Median 230.63 95.0% UCL of Median 1987.94 4/10/2013 10:40:41 AM 1244 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-09 Reach=R05, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 41 119 19 34 Multiplicity Factor = 0 Mean of W 108 45 Std Dev of W 9.486833 9.486833 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.278604 No 0.860698 No 0.139302 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.1595 0.246252 No -1.1595 0.876874 No -1.1595 0.123126 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.1068 0.268382 No -1.2122 0.887283 No -1.1068 0.134191 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.500000 0.050000 0.500000 Reject H0 if Greater Than 0.6544 0.6544 0.6544 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.2675 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:42 AM 1245 4/10/2013 10:40:49 AM 1306 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Apr-12 26 1516.2 1450.952 284.5552 930.1476 2102.252 Reach=R05, Date=Nov-12 5 404.88 192.5162 86.09584 165.8396 643.9204 Note: T-alpha (Reach=R05, Date=Apr-12) = 2.0595, T-alpha (Reach=R05, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 29 1111.32 1349.072 Unequal 28.30 1111.32 1463.668 Note: T-alpha (Equal) = 2.0452, T-alpha (Unequal) = 2.0474 Standard Error 658.7856 297.2947 95.0% LCL Difference -236.048 502.6342 95.0% UCL Difference 2458.688 1720.006 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.6869 0.102348 No Difference < 0 1.6869 0.948826 No Difference > 0 1.6869 0.051174 No 73 o cn DCD CD II Difference: (Reach =R05, Date=Apr-12)-(Reach= Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.371299 0.000495 0.501017 Power (Alpha=.010) 0.162609 0.000041 0.236564 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.7381 0.000834 Yes Difference < 0 3.7381 0.999583 No Difference > 0 3.7381 0.000417 Yes Difference: (Reach=R05, Date=Apr-12)-(Reach=R05, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.950380 0.000000 0.977345 Power (Alpha=.010) 0.826539 0.000000 0.890666 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Apr-12) Kurtosis Normality (Reach=R05, Date=Apr-12) Omnibus Normality (Reach=R05, Date=Apr-12) Skewness Normality (Reach=R05, Date=Nov-12) Kurtosis Normality (Reach=R05, Date=Nov-12) Omnibus Normality (Reach=R05, Date=Nov-12) Variance-Ratio Equal-Variance Test 56.8031 Modified-Levene Equal-Variance Test 3.3468 Probability 2.3380 0.4085 5.6331 0.0000 Decision(.050) 0.019388 Reject normality 0.682909 Cannot reject normality 0.059813 Cannot reject normality 1.000000 Cannot reject normality 0.001303 0.077634 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Apr-12 Reach=R05, Date=Nov-12 Count 26 5 Median 852 435 95.0% LCL of Median 526 95.0% UCL of Median 2020 4/10/2013 10:40:49 AM 1307 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Apr-12 Reach=R05, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 102 453 28 43 Multiplicity Factor = 0 Mean of W 41 6 80 Std Dev of W 18.61899 18.61899 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000000 Yes 0.000000 Yes 0.000000 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.9872 0.046898 Yes -1.9872 0.976551 No -1.9872 0.023449 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.9604 0.049953 Yes -2.0141 0.977999 No -1.9604 0.024977 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.653846 0.076923 0.653846 Reject H0 if Greater Than 0.6085 0.6085 0.6085 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0306 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:49 AM 1308 4/10/2013 10:41:05 AM 1429 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R06, Date=Jun-02 4 163 260.0933 130.0466 -250.8664 576.8664 Reach=R06, Date=Nov-04 5 68.72 23.02981 10.29924 40.12472 97.31528 Note: T-alpha (Reach=R06, Date=Jun-02) = 3.1824, T-alpha (Reach=R06, Date=Nov-04) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 7 94.28 171.1586 Unequal 3.04 94.28 261.1108 Note: T-alpha (Equal) = 2.3646, T-alpha (Unequal) = 3.1602 Standard Error 114.8167 130.4538 95.0% LCL Difference -177.2184 -317.9855 95.0% UCL Difference 365.7784 506.5455 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.8211 0.438636 No Difference < 0 0.8211 0.780682 No Difference > 0 0.8211 0.219318 No Difference: (Reach=R06, Date=Jun-02)-(Reach=R06, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.110118 0.008352 0.183719 Power (Alpha=.010) 0.027691 0.001287 0.049552 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7227 0.521511 No Difference < 0 0.7227 0.739245 No Difference > 0 0.7227 0.260755 No Difference: (Reach=R06, Date=Jun-02)-(Reach=R06, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.082235 0.012657 0.142419 Power (Alpha=.010) 0.017616 0.002308 0.032181 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R06, Date=Jun-02) Kurtosis Normality (Reach=R06, Date=Jun-02) Omnibus Normality (Reach=R06, Date=Jun-02) Skewness Normality (Reach=R06, Date=Nov-04) Kurtosis Normality (Reach=R06, Date=Nov-04) Omnibus Normality (Reach=R06, Date=Nov-04) Variance-Ratio Equal-Variance Test 127.5492 Modified-Levene Equal-Variance Test 2.1063 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.000400 0.189984 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06, Date=Jun-02 Reach=R06, Date=Nov-04 Count 4 5 Median 51.25 72.4 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:41:05 AM 1430 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06, Date=Jun-02 Reach=R06, Date=Nov-04 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 10 20 10 25 Multiplicity Factor = 6 Mean of W 20 25 Std Dev of W 4.065437 4.065437 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.0000 1.000000 No 0.0000 0.500000 No 0.0000 0.500000 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.1230 1.000000 No 0.1230 0.548942 No -0.1230 0.548942 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.500000 0.500000 0.500000 Reject H0 if Greater Than 0.7824 0.7824 0.7824 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.5635 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:05 AM 1431 Normal Probability Plot of Larcemouth_Bass_P-0_ppb when Reach=R03, Date=N Percent ofValies Reach=RD6, Date.=Jun-02 Reach=R06, Date=Nov-C4 Groins 4/10/2013 10:41:05 AM 1432 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R06, Date=Jun-02 4 163 260.0933 130.0466 -250.8664 576.8664 Reach=R06, Date=Nov-12 5 65.46 32.25117 14.42316 25.41488 105.5051 Note: T-alpha (Reach=R06, Date=Jun-02) = 3.1824, T-alpha (Reach=R06, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 7 97.54 172.0075 Unequal 3.07 97.54 262.0852 Note: T-alpha (Equal) = 2.3646, T-alpha (Unequal) = 3.1396 Standard Error 115.3861 130.844 95.0% LCL Difference -175.3049 -313.257 95.0% UCL Difference 370.3849 508.337 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.8453 0.425871 No Difference < 0 0.8453 0.787065 No Difference > 0 0.8453 0.212935 No Difference: (Reach=R06, Date=Jun-02)-(Reach=R06, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.113787 0.007862 0.189581 Power (Alpha=.010) 0.028859 0.001203 0.051611 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7455 0.508888 No Difference < 0 0.7455 0.745556 No Difference > 0 0.7455 0.254444 No Difference: (Reach=R06, Date=Jun-02)-(Reach=R06, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.084551 0.012013 0.146836 Power (Alpha=.010) 0.018202 0.002181 0.033397 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R06, Date=Jun-02) Kurtosis Normality (Reach=R06, Date=Jun-02) Omnibus Normality (Reach=R06, Date=Jun-02) Skewness Normality (Reach=R06, Date=Nov-12) Kurtosis Normality (Reach=R06, Date=Nov-12) Omnibus Normality (Reach=R06, Date=Nov-12) Variance-Ratio Equal-Variance Test 65.0380 Modified-Levene Equal-Variance Test 1.9987 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.001503 0.200332 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06, Date=Jun-02 Reach=R06, Date=Nov-12 Count 4 5 Median 51.25 50.6 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:41:06 AM 1433 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06, Date=Jun-02 Reach=R06, Date=Nov-12 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 9 19 11 26 Multiplicity Factor = 6 Mean of W 20 25 Std Dev of W 4.065437 4.065437 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.2460 0.805701 No -0.2460 0.402850 No -0.2460 0.597150 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.1230 0.902117 No -0.1230 0.451058 No -0.3690 0.643923 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.500000 0.500000 0.300000 Reject H0 if Greater Than 0.7824 0.7824 0.7824 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.5635 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:06 AM 1434 Reach=RD6, Date.=Jun-02 Reach=R06, Date=Nov-12 Groins 4/10/2013 10:41:13 AM 1486 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R06, Date=Nov-04 5 68.72 23.02981 10.29924 40.12472 97.31528 Reach=R06, Date=Nov-12 5 65.46 32.25117 14.42316 25.41488 105.5051 Note: T-alpha (Reach=R06, Date=Nov-04) = 2.7764, T-alpha (Reach=R06, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 3.26 28.0224 Unequal 7.24 3.26 39.62966 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3490 Standard Error 17.72292 17.72292 95.0% LCL Difference -37.60913 -38.37089 95.0% UCL Difference 44.12914 44.89089 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.1839 0.858635 No Difference < 0 0.1839 0.570682 No Difference > 0 0.1839 0.429318 No Difference: (Reach=R06, Date=Nov-04)-(Reach=R06, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.053050 0.034836 0.069991 Power (Alpha=.010) 0.010848 0.006516 0.014993 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.1839 0.859106 No Difference < 0 0.1839 0.570447 No Difference > 0 0.1839 0.429553 No Difference: (Reach=R06, Date=Nov-04)-(Reach=R06, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.052973 0.034948 0.069791 Power (Alpha=.010) 0.010814 0.006562 0.014897 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R06, Date=Nov-04) Kurtosis Normality (Reach=R06, Date=Nov-04) Omnibus Normality (Reach=R06, Date=Nov-04) Skewness Normality (Reach=R06, Date=Nov-12) Kurtosis Normality (Reach=R06, Date=Nov-12) Omnibus Normality (Reach=R06, Date=Nov-12) Variance-Ratio Equal-Variance Test 1.9611 Modified-Levene Equal-Variance Test 0.0327 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.530219 0.861029 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06, Date=Nov-04 Reach=R06, Date=Nov-12 Count 5 5 Median 72.4 50.6 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:41:13 AM 1487 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06, Date=Nov-04 Reach=R06, Date=Nov-12 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 14.5 29.5 10.5 25.5 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.4191 0.675174 No 0.4191 0.662413 No 0.4191 0.337587 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.3143 0.753298 No 0.5238 0.699799 No 0.3143 0.376649 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section 0-i|---------------------------- Histogram 30 20- 10- 3-H----------- 1----------- ----------- 1----------- ------------1----------- 10 5C 60 70 80 90 100 Largemouth_0as5_PFCJ5_ppfci w h e n R e a c h = R 0 6 , D ate= 'lov-O l Largem ojth_Bass_FFCS_ppb w hen Reach=R03, Date=Nov-12 Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:13 AM 1488 4/10/2013 10:41:41 AM 1684 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R09, Date=Jun-02 6 553.5 231.1075 94.34927 310.9675 796.0325 Reach=R09, Date=Nov-04 5 477.6 178.4959 79.82581 255.968 Note: T-alpha (Reach=R09, Date=Jun-02) = 2.5706, T-alpha (Reach=R09, Date=Nov-04) = 2.7764 699.232 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 75.9 209.3632 Unequal 8.97 75.9 292.0128 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 2.2632 Standard Error 126.7757 123.5878 95.0% LCL Difference -210.8866 -203.8033 95.0% UCL Difference 362.6866 355.6033 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.5987 0.564139 No Difference < 0 0.5987 0.717931 No Difference > 0 0.5987 0.282069 No Difference: (Reach=R09, Date=Jun-02)-(Reach=R09, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.083688 0.013864 0.137823 Power (Alpha=.010) 0.019973 0.002215 0.035099 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6141 0.554373 No Difference < 0 0.6141 0.722813 No Difference > 0 0.6141 0.277187 No Difference: (Reach=R09, Date=Jun-02)-(Reach=R09, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.085451 0.013368 0.140964 Power (Alpha=.010) 0.020517 0.002124 0.036120 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R09, Date=Jun-02) Kurtosis Normality (Reach=R09, Date=Jun-02) Omnibus Normality (Reach=R09, Date=Jun-02) Skewness Normality (Reach=R09, Date=Nov-04) Kurtosis Normality (Reach=R09, Date=Nov-04) Omnibus Normality (Reach=R09, Date=Nov-04) Variance-Ratio Equal-Variance Test 1.6764 Modified-Levene Equal-Variance Test 0.0931 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.636902 0.767184 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09, Date=Jun-02 Reach=R09, Date=Nov-04 Count 6 5 Median 629 432 95.0% LCL of Median 152 95.0% UCL of Median 788 4/10/2013 10:41:41 AM 1685 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09, Date=Jun-02 Reach=R09, Date=Nov-04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 19 40 11 26 Multiplicity Factor = 0 Mean of W 36 30 Std Dev of W 5.477226 5.477226 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.536797 No 0.731602 No 0.268398 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.7303 0.465209 No -0.7303 0.767396 No -0.7303 0.232604 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.6390 0.522817 No -0.8216 0.794343 No -0.6390 0.261408 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.466667 0.166667 0.466667 Reject H0 if Greater Than 0.7246 0.7246 0.7246 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.4740 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:41 AM 1686 4/10/2013 10:41:41 AM 1687 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R09, Date=Jun-02 6 553.5 231.1075 94.34927 310.9675 796.0325 Reach=R09, Date=Dec-06 5 304.6 63.22025 28.27295 226.1017 383.0983 Note: T-alpha (Reach=R09, Date=Jun-02) = 2.5706, T-alpha (Reach=R09, Date=D ec-06) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 248.9 177.3386 Unequal 5.88 248.9 239.5986 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 2.4592 Standard Error 107.3838 98.49438 95.0% LCL Difference 5.980891 6.685791 95.0% UCL Difference 491.8191 491.1142 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3179 0.045646 Yes Difference < 0 2.3179 0.977177 No Difference > 0 2.3179 0.022823 Yes Difference: (Reach=R09, Date=Jun-02)-(Reach=R09, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.543127 0.000070 0.689359 Power (Alpha=.010) 0.252154 0.000007 0.362963 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.5270 0.045656 Yes Difference < 0 2.5270 0.977172 No Difference > 0 2.5270 0.022828 Yes Difference: (Reach=R09, Date=Jun-02)-(Reach=R09, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.559269 0.000042 0.718655 Power (Alpha=.010) 0.238108 0.000005 0.358842 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R09, Date=Jun-02) Kurtosis Normality (Reach=R09, Date=Jun-02) Omnibus Normality (Reach=R09, Date=Jun-02) Skewness Normality (Reach=R09, Date=Dec-06) Kurtosis Normality (Reach=R09, D ate=Dec-06) Omnibus Normality (Reach=R09, Date=Dec-06) Variance-Ratio Equal-Variance Test 13.3634 Modified-Levene Equal-Variance Test 2.0903 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.026356 0.182143 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09, Date=Jun-02 Reach=R09, Date=Dec-06 Count 6 5 Median 629 296 95.0% LCL of Median 152 95.0% UCL of Median 788 4/10/2013 10:41:41 AM 1688 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09, Date=Jun-02 Reach=R09, Date=Dec-06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 46 5 20 Multiplicity Factor = 0 Mean of W 36 30 Std Dev of W 5.477226 5.477226 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.082251 No 0.958874 No 0.041126 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 -1.8257 0.067889 No -1.8257 0.966055 No -1.8257 0.033945 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.7345 0.082837 No -1.9170 0.972383 No -1.7345 0.041419 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.833333 0.166667 0.833333 Reject H0 if Greater Than 0.7246 0.7246 0.7246 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0260 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:41 AM 1689 4/10/2013 10:41:41 AM 1690 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R09, Date=Jun-02 6 553.5 231.1075 94.34927 310.9675 796.0325 Reach=R09, Date=Nov-12 5 231.8 57.10692 25.53899 160.8924 302.7076 Note: T-alpha (Reach=R09, Date=Jun-02) = 2.5706, T-alpha (Reach=R09, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 321.7 176.4144 Unequal 5.72 321.7 238.0586 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 2.4761 Standard Error 106.8242 97.74468 95.0% LCL Difference 80.04684 79.67306 95.0% UCL Difference 563.3531 563.7269 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.0115 0.014680 Yes Difference < 0 3.0115 0.992660 No Difference > 0 3.0115 0.007340 Yes Difference: (Reach=R09, Date=Jun-02)-(Reach=R09, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.764280 0.000004 0.870683 Power (Alpha=.010) 0.456148 0.000000 0.590976 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.2912 0.017783 Yes Difference < 0 3.2912 0.991109 No Difference > 0 3.2912 0.008891 Yes Difference: (Reach=R09, Date=Jun-02)-(Reach=R09, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.774408 0.000002 0.890212 Power (Alpha=.010) 0.420116 0.000000 0.574363 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R09, Date=Jun-02) Kurtosis Normality (Reach=R09, Date=Jun-02) Omnibus Normality (Reach=R09, Date=Jun-02) Skewness Normality (Reach=R09, Date=Nov-12) Kurtosis Normality (Reach=R09, Date=Nov-12) Omnibus Normality (Reach=R09, Date=Nov-12) Variance-Ratio Equal-Variance Test 16.3776 Modified-Levene Equal-Variance Test 2.1033 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.018102 0.180918 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09, Date=Jun-02 Reach=R09, Date=Nov-12 Count 6 5 Median 629 259 95.0% LCL of Median 152 95.0% UCL of Median 788 4/10/2013 10:41:41 AM 1691 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09, Date=Jun-02 Reach=R09, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 46 5 20 Multiplicity Factor = 0 Mean of W 36 30 Std Dev of W 5.477226 5.477226 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.082251 No 0.958874 No 0.041126 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 -1.8257 0.067889 No -1.8257 0.966055 No -1.8257 0.033945 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.7345 0.082837 No -1.9170 0.972383 No -1.7345 0.041419 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.833333 0.166667 0.833333 Reject H0 if Greater Than 0.7246 0.7246 0.7246 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0260 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:42 AM 1692 Normal Probability Plot of Larcemouth_Bass_P-0_ppb when Reach=R03, Date=N Percent ofValies 4/10/2013 10:41:46 AM 1726 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R09, Date=Nov-04 5 477.6 178.4959 79.82581 255.968 Reach=R09, Date=Dec-06 5 304.6 63.22025 28.27295 226.1017 383.0983 Note: T-alpha (Reach=R09, Date=Nov-04) = 2.7764, T-alpha (Reach=R09, Date=D ec-06) = 2.7764 699.232 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 173 133.8985 Unequal 4.99 173 189.361 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5724 Standard Error 84.68483 84.68483 95.0% LCL Difference -22.28356 -44.84666 95.0% UCL Difference 368.2836 390.8466 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0429 0.075338 No Difference < 0 2.0429 0.962331 No Difference > 0 2.0429 0.037669 Yes Difference: (Reach=R09, Date=Nov-04)-(Reach=R09, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.435704 0.000205 0.587730 Power (Alpha=.010) 0.175841 0.000022 0.268495 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0429 0.096651 No Difference < 0 2.0429 0.951674 No Difference > 0 2.0429 0.048326 Yes Difference: (Reach=R09, Date=Nov-04)-(Reach=R09, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.380669 0.000271 0.545626 Power (Alpha=.010) 0.128235 0.000036 0.212501 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R09, Date=Nov-04) Kurtosis Normality (Reach=R09, Date=Nov-04) Omnibus Normality (Reach=R09, Date=Nov-04) Skewness Normality (Reach=R09, Date=Dec-06) Kurtosis Normality (Reach=R09, D ate=Dec-06) Omnibus Normality (Reach=R09, Date=Dec-06) Variance-Ratio Equal-Variance Test 7.9716 Modified-Levene Equal-Variance Test 2.6741 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.069005 0.140631 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09, Date=Nov-04 Reach=R09, Date=Dec-06 Count 5 5 Median 432 296 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:41:46 AM 1727 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09, Date=Nov-04 Reach=R09, Date=Dec-06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 22 37 3 18 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.972222 No 0.027778 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 1.9845 0.047202 Yes 1.9845 0.976399 No 1.9845 0.023601 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.8800 0.060103 No 2.0889 0.981643 No 1.8800 0.030051 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:47 AM 1728 200 -I-------------------- T--------------------------------------------------------------------------T----------------------- Reach=R09, Cat3=Ncv-04 Reach=R09, Date=De>06 Groins 4/10/2013 10:41:47 AM 1729 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R09, Date=Nov-04 5 477.6 178.4959 79.82581 255.968 Reach=R09, Date=Nov-12 5 231.8 57.10692 25.53899 160.8924 302.7076 Note: T-alpha (Reach=R09, Date=Nov-04) = 2.7764, T-alpha (Reach=R09, Date=Nov-12) = 2.7764 699.232 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 245.8 132.5179 Unequal 4.81 245.8 187.4086 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6014 Standard Error 83.81169 83.81169 95.0% LCL Difference 52.52989 27.77545 95.0% UCL Difference 439.0701 463.8246 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9328 0.018918 Yes Difference < 0 2.9328 0.990541 No Difference > 0 2.9328 0.009459 Yes Difference: (Reach=R09, Date=Nov-04)-(Reach=R09, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.729129 0.000006 0.847972 Power (Alpha=.010) 0.406932 0.000001 0.544072 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9328 0.034083 Yes Difference < 0 2.9328 0.982958 No Difference > 0 2.9328 0.017042 Yes Difference: (Reach=R09, Date=Nov-04)-(Reach=R09, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.644914 0.000010 0.801810 Power (Alpha=.010) 0.282202 0.000001 0.423805 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R09, Date=Nov-04) Kurtosis Normality (Reach=R09, Date=Nov-04) Omnibus Normality (Reach=R09, Date=Nov-04) Skewness Normality (Reach=R09, Date=Nov-12) Kurtosis Normality (Reach=R09, Date=Nov-12) Omnibus Normality (Reach=R09, Date=Nov-12) Variance-Ratio Equal-Variance Test 9.7697 Modified-Levene Equal-Variance Test 2.6803 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.048528 0.140231 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09, Date=Nov-04 Reach=R09, Date=Nov-12 Count 5 5 Median 432 259 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:41:47 AM 1730 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09, Date=Nov-04 Reach=R09, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:47 AM 1731 Norrral Probability' Piet of Largemou:h_Bass_PFOS_ppb when Reaoh=R09, Date=N Normal Prooability Plot of Larcemouth_Bass_P-0_ppb when Reach=R03, Date=N 1 750 10 25 50 75 Percent ofValues 90 95 Largernouth_Bass_PFOS_ppbvs Grouos 39 Percent ofValies 150 Reach-R09, 0ata-Ncv-04 Reach=R09, Date-No-/-12 Grouos 4/10/2013 10:41:52 AM 1765 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R09, Date=Dec-06 5 304.6 63.22025 28.27295 226.1017 383.0983 Reach=R09, Date=Nov-12 5 231.8 57.10692 25.53899 160.8924 302.7076 Note: T-alpha (Reach=R09, Date=D ec-06) = 2.7764, T-alpha (Reach=R09, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 72.8 60.24118 Unequal 7.92 72.8 85.19389 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3101 Standard Error 38.09987 38.09987 95.0% LCL Difference -15.05845 -15.21579 95.0% UCL Difference 160.6585 160.8158 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1 .91 08 0.092422 No Difference < 0 1.91 08 0.953789 No Difference > 0 1.9108 0.046211 Yes Difference: (Reach=R09, Date=Dec-06)-(Reach=R09, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.390913 0.000323 0.540500 Power (Alpha=.010) 0.150187 0.000035 0.233835 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1 .91 08 0.092798 No Difference < 0 1.91 08 0.953601 No Difference > 0 1.9108 0.046399 Yes Difference: (Reach=R09, Date=Dec-06)-(Reach=R09, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.390034 0.000325 0.539826 Power (Alpha=.010) 0.149382 0.000036 0.232917 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R09, Date=Dec-06) Kurtosis Normality (Reach=R09, D ate=Dec-06) Omnibus Normality (Reach=R09, Date=Dec-06) Skewness Normality (Reach=R09, Date=Nov-12) Kurtosis Normality (Reach=R09, Date=Nov-12) Omnibus Normality (Reach=R09, Date=Nov-12) Variance-Ratio Equal-Variance Test 1.2256 Modified-Levene Equal-Variance Test 0.0005 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.848495 0.982124 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09, Date=Dec-06 Reach=R09, Date=Nov-12 Count 5 5 Median 296 259 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:41:52 AM 1766 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09, Date=Dec-06 Reach=R09, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 19 34 6 21 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.888889 No 0.111111 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.3578 0.174525 No 1.3578 0.912737 No 1.3578 0.087263 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.2534 0.210075 No 1.4623 0.928164 No 1.2534 0.105038 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:52 AM 1767 Normal Probability Plot of Larcemouth_Bass_P-0_ppb when Reach=R03, Date=N Percent ofValies 160 Reach=R09 Date=Dec-06 Reach=R09, Date=Nov-12 Groins 4/10/2013 10:42:05 AM 1864 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R11, Date=Nov-04 5 356.28 351.4388 157.1682 -80.08884 792.6489 Reach=R11, Date=Dec-06 5 298.4 39.52594 17.67654 249.3221 347.4779 Note: T-alpha (R each=R 11, Date=Nov-04) = 2.7764, T-alpha (R each=R 11, Date=D ec-06) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 57.88 250.0715 Unequal 4.10 57.88 353.6545 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7496 Standard Error 158.1591 158.1591 95.0% LCL Difference -306.8355 -377.0004 95.0% UCL Difference 422.5955 492.7604 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.3660 0.723883 No Difference < 0 0.3660 0.638059 No Difference > 0 0.3660 0.361941 No Difference: (Reach=R11, D ate=N ov-04)-(R each=R 11, D ate=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.062129 0.023760 0.095300 Power (Alpha=.010) 0.013418 0.004168 0.021887 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.3660 0.732493 No Difference < 0 0.3660 0.633753 No Difference > 0 0.3660 0.366247 No Difference: (Reach=R11, D ate=N ov-04)-(R each=R 11, D ate=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.059653 0.025143 0.090987 Power (Alpha=.010) 0.012393 0.004661 0.019804 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R11, Date=Nov-04) Kurtosis Normality (Reach=R11, Date=Nov-04) Omnibus Normality (Reach=R11, Date=Nov-04) Skewness Normality (Reach=R11, Date=Dec-06) Kurtosis Normality (Reach=R11, D ate=Dec-06) Omnibus Normality (Reach=R11, Date=Dec-06) Variance-Ratio Equal-Variance Test 79.0560 Modified-Levene Equal-Variance Test 2.7150 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.000928 0.138024 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11, Date=Nov-04 Reach=R11, Date=Dec-06 Count 5 5 Median 147 295 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:42:05 AM 1865 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11, Date=Nov-04 Reach=R11, Date=Dec-06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 10 25 15 30 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.690476 No 0.345238 No 0.654762 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.5222 0.601508 No -0.5222 0.300754 No -0.5222 0.699246 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.4178 0.676103 No -0.4178 0.338052 No -0.6267 0.734565 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:42:05 AM Norrral Probability' Piet of Largemou:h_Bass_PFGS_ppb when Reaoh=R11, Date=N 1866 1 900 n 10 25 50 75 Percent ofValues 90 95 Largernouth_Bass_PFOS_ppbvs Grouos 39 750 oJ-----------------1---------------------------------- 1---------- Reach=R11, Cat3=Ncv-04 Reach=R11, Date=De>06 Grouos 4/10/2013 10:42:06 AM 1867 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R11, Date=Nov-04 5 356.28 351.4388 157.1682 -80.08884 792.6489 Reach=R11, Date=Nov-12 5 162.2 52.16991 23.33109 97.42249 226.9775 Note: T-alpha (R each=R 11, Date=Nov-04) = 2.7764, T-alpha (R each=R 11, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 194.08 251.2279 Unequal 4.18 194.08 355.2899 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7308 Standard Error 158.8905 158.8905 95.0% LCL Difference -172.3221 -239.826 95.0% UCL Difference 560.4821 627.986 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.2215 0.256679 No Difference < 0 1.2215 0.871660 No Difference > 0 1.2215 0.128340 No Difference: (Reach=R11, Date=Nov-04)-(Reach=R11, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.190379 0.002788 0.299419 Power (Alpha=.010) 0.056702 0.000371 0.097128 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.2215 0.286344 No Difference < 0 1.2215 0.856828 No Difference > 0 1.2215 0.143172 No Difference: (Reach=R11, Date=Nov-04)-(Reach=R11, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.160594 0.003462 0.268861 Power (Alpha=.010) 0.040479 0.000552 0.074244 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R11, Date=Nov-04) Kurtosis Normality (Reach=R11, Date=Nov-04) Omnibus Normality (Reach=R11, Date=Nov-04) Skewness Normality (Reach=R11, Date=Nov-12) Kurtosis Normality (Reach=R11, Date=Nov-12) Omnibus Normality (Reach=R11, Date=Nov-12) Variance-Ratio Equal-Variance Test 45.3794 Modified-Levene Equal-Variance Test 2.4213 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.002749 0.158304 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11, Date=Nov-04 Reach=R11, Date=Nov-12 Count 5 5 Median 147 145 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:42:06 AM 1868 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11, Date=Nov-04 Reach=R11, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 15 30 10 25 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.690476 No 0.654762 No 0.345238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.5222 0.601508 No 0.5222 0.699246 No 0.5222 0.300754 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.4178 0.676103 No 0.6267 0.734565 No 0.4178 0.338052 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:42:06 AM 1869 Norrral Probability' Piet of Largemou:h_Bass_PFGS_ppb when Reaoh=R11, Date=N Normal Prooability Plot of Larcemouth_Bass_P-0_ppb when Reach=R11. Date=N 1 900 n 10 25 50 75 Percent ofValues 90 95 Largernouth_Bass_PFOS_ppbvs Grouos 39 750 Percent ofValies oJ-----------------1---------------------------------- 1---------- Reach=R11, 0at3=Ncv-04 Reach=R' 1, Date-Nov-12 Grouos 4/10/2013 10:42:09 AM 1891 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R11, Date=Dec-06 5 298.4 39.52594 17.67654 249.3221 347.4779 Reach=R11, Date=Nov-12 5 162.2 52.16991 23.33109 97.42249 226.9775 Note: T-alpha (R each=R 11, Date=D ec-06) = 2.7764, T-alpha (R each=R 11, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 136.2 46.28175 Unequal 7.45 136.2 65.45227 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3357 Standard Error 29.27115 29.27115 95.0% LCL Difference 68.70061 67.83031 95.0% UCL Difference 203.6994 204.5697 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 4.6530 0.001638 Yes Difference < 0 4.6530 0.999181 No Difference > 0 4.6530 0.000819 Yes Difference: (Reach=R11, Date=Dec-06)-(Reach=R11, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.981483 0.000000 0.994980 Power (Alpha=.010) 0.859545 0.000000 0.932206 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 4.6530 0.001976 Yes Difference < 0 4.6530 0.999012 No Difference > 0 4.6530 0.000988 Yes Difference: (Reach=R11, Date=Dec-06)-(Reach=R11, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.979261 0.000000 0.994461 Power (Alpha=.010) 0.843197 0.000000 0.923810 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R11, Date=Dec-06) Kurtosis Normality (Reach=R11, D ate=Dec-06) Omnibus Normality (Reach=R11, Date=Dec-06) Skewness Normality (Reach=R11, Date=Nov-12) Kurtosis Normality (Reach=R11, Date=Nov-12) Omnibus Normality (Reach=R11, Date=Nov-12) Variance-Ratio Equal-Variance Test 1.7421 Modified-Levene Equal-Variance Test 0.4755 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.603959 0.509959 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11, Date=Dec-06 Reach=R11, Date=Nov-12 Count 5 5 Median 295 145 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:42:09 AM 1892 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11, Date=Dec-06 Reach=R11, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:42:09 AM 1893 Norrral Probability' Piet of Largemou:h_Bass_PFGS_ppb when Reaoh=R11, Data=D 3Z0-i Normal Prooability Plot of Larcemouth_Bass_R-Q_ppb when Reach=R11. Date=N Forcent ofValues Largernouth_Bass_PFOS_ppbvs Grouos Percent ofValies too-I-----------------t---------------------------------- T---------- Reach=R11 Date=Dec-06 Reach=R` 1, Date=Nov-12 Grouos 4/10/2013 10:42:17 AM 1954 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R13, Date=Jul-07 6 148.85 56.32444 22.99436 89.74112 207.9589 Reach=R13, Date=Jul-08 6 86.36833 29.88 12.19846 55.01119 117.7255 Note: T-alpha (Reach=R13, Date=Jul-07) = 2.5706, T-alpha (Reach=R13, Date=Jul-08) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 62.48167 45.08469 Unequal 7.61 62.48167 63.75937 Note: T-alpha (Equal) = 2.2281, T-alpha (Unequal) = 2.3269 Standard Error 26.02966 26.02966 95.0% LCL Difference 4.48398 1.914178 95.0% UCL Difference 120.4794 123.0492 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.4004 0.037290 Yes Difference < 0 2.4004 0.981355 No Difference > 0 2.4004 0.018645 Yes Difference: (Reach=R13, Date=Jul-07)-(Reach=R13, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.582066 0.000048 0.721972 Power (Alpha=.010) 0.287453 0.000004 0.402545 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.4004 0.044688 Yes Difference < 0 2.4004 0.977656 No Difference > 0 2.4004 0.022344 Yes Difference: (Reach=R13, Date=Jul-07)-(Reach=R13, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.553344 0.000057 0.703690 Power (Alpha=.010) 0.250537 0.000006 0.365722 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R13, Date=Jul-07) Kurtosis Normality (Reach=R13, Date=Jul-07) Omnibus Normality (Reach=R13, Date=Jul-07) Skewness Normality (Reach=R13, Date=Jul-08) Kurtosis Normality (Reach=R13, Date=Jul-08) Omnibus Normality (Reach=R13, Date=Jul-08) Variance-Ratio Equal-Variance Test 3.5533 Modified-Levene Equal-Variance Test 0.5048 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.190349 0.493618 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-07 Reach=R13, Date=Jul-08 Count 6 6 Median 139.6 82.655 95.0% LCL of Median 86.7 59.5 95.0% UCL of Median 254.7 142.18 4/10/2013 10:42:18 AM 1955 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13, Date=Jul-07 Reach=R13, Date=Jul-08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 31 52 5 26 Multiplicity Factor = 0 Mean of W 39 39 Std Dev of W 6.244998 6.244998 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.041126 Yes 0.979437 No 0.020563 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.0817 0.037373 Yes 2.0817 0.981314 No 2.0817 0.018686 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.0016 0.045328 Yes 2.1617 0.984681 No 2.0016 0.022664 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.666667 0.000000 0.666667 Reject H0 if Greater Than 0.6980 0.6980 0.6980 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.1429 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:42:18 AM 1956 4/10/2013 10:42:18 AM 1957 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R13, Date=Jul-07 6 148.85 56.32444 22.99436 89.74112 207.9589 Reach=R13, Date=Jul-09 6 242.6817 254.7418 103.9979 -24.65345 510.0168 Note: T-alpha (Reach=R13, Date=Jul-07) = 2.5706, T-alpha (Reach=R13, Date=Jul-09) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -93.83167 184.4801 Unequal 5.49 -93.83167 260.8943 Note: T-alpha (Equal) = 2.2281, T-alpha (Unequal) = 2.5034 Standard Error 106.5096 106.5096 95.0% LCL Difference -331.1499 -360.4641 95.0% UCL Difference 143.4866 172.8008 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.8810 0.399013 No Difference < 0 -0.8810 0.199507 No Difference > 0 -0.8810 0.800493 No Difference: (Reach=R13, Date=Jul-07)-(Reach=R13, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.125710 0.205325 0.006761 Power (Alpha=.010) 0.034136 0.059695 0.000961 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.8810 0.415247 No Difference < 0 -0.8810 0.207623 No Difference > 0 -0.8810 0.792377 No Difference: (Reach=R13, Date=Jul-07)-(Reach=R13, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.114067 0.192341 0.007570 Power (Alpha=.010) 0.028018 0.050827 0.001204 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R13, Date=Jul-07) Kurtosis Normality (Reach=R13, Date=Jul-07) Omnibus Normality (Reach=R13, Date=Jul-07) Skewness Normality (Reach=R13, Date=Jul-09) Kurtosis Normality (Reach=R13, Date=Jul-09) Omnibus Normality (Reach=R13, Date=Jul-09) Variance-Ratio Equal-Variance Test 20.4553 Modified-Levene Equal-Variance Test 0.7570 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.004843 0.404659 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-07 Reach=R13, Date=Jul-09 Count 6 6 Median 139.6 138.37 95.0% LCL of Median 86.7 116.85 95.0% UCL of Median 254.7 760 4/10/2013 10:42:18 AM 1958 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13, Date=Jul-07 Reach=R13, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 16 37 20 41 Multiplicity Factor = 0 Mean of W 39 39 Std Dev of W 6.244998 6.244998 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.818182 No 0.409091 No 0.590909 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.3203 0.748774 No -0.3203 0.374387 No -0.3203 0.625613 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.2402 0.810181 No -0.2402 0.405091 No -0.4003 0.655540 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.333333 0.333333 0.166667 Reject H0 if Greater Than 0.6980 0.6980 0.6980 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.9307 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:42:18 AM 1959 4/10/2013 10:42:18 AM 1960 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R13, Date=Jul-07 6 148.85 56.32444 22.99436 89.74112 207.9589 Reach=R13, Date=Nov-12 5 77.02 12.92892 5.781989 60.96663 93.07338 Note: T-alpha (Reach=R13, Date=Jul-07) = 2.5706, T-alpha (Reach=R13, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 71.83 42.85744 Unequal 5.62 71.83 57.78927 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 2.4871 Standard Error 25.95147 23.71017 95.0% LCL Difference 13.1237 12.86045 95.0% UCL Difference 130.5363 130.7995 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7679 0.021830 Yes Difference < 0 2.7679 0.989085 No Difference > 0 2.7679 0.010915 Yes Difference: (Reach=R13, Date=Jul-07)-(Reach=R13, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.693427 0.000012 0.817694 Power (Alpha=.010) 0.380063 0.000001 0.510636 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.0295 0.025063 Yes Difference < 0 3.0295 0.987468 No Difference > 0 3.0295 0.012532 Yes Difference: (Reach=R13, Date=Jul-07)-(Reach=R13, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.703505 0.000006 0.840456 Power (Alpha=.010) 0.347097 0.000001 0.493821 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R13, Date=Jul-07) Kurtosis Normality (Reach=R13, Date=Jul-07) Omnibus Normality (Reach=R13, Date=Jul-07) Skewness Normality (Reach=R13, Date=Nov-12) Kurtosis Normality (Reach=R13, Date=Nov-12) Omnibus Normality (Reach=R13, Date=Nov-12) Variance-Ratio Equal-Variance Test 18.9788 Modified-Levene Equal-Variance Test 1.5629 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.013740 0.242770 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-07 Reach=R13, Date=Nov-12 Count 6 5 Median 139.6 71 95.0% LCL of Median 86.7 95.0% UCL of Median 254.7 4/10/2013 10:42:18 AM 1961 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13, Date=Jul-07 Reach=R13, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 29 50 1 16 Multiplicity Factor = 0 Mean of W 36 30 Std Dev of W 5.477226 5.477226 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.008658 Yes 0.995671 No 0.004329 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.5560 0.010587 Yes -2.5560 0.994706 No -2.5560 0.005294 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.4648 0.013711 Yes -2.6473 0.995943 No -2.4648 0.006855 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.833333 0.000000 0.833333 Reject H0 if Greater Than 0.7246 0.7246 0.7246 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0260 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:42:18 AM 1962 4/10/2013 10:42:19 AM 1969 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R13, Date=Jul-08 6 86.36833 29.88 12.19846 55.01119 117.7255 Reach=R13, Date=Jul-09 6 242.6817 254.7418 103.9979 -24.65345 510.0168 Note: T-alpha (Reach=R13, Date=Jul-08) = 2.5706, T-alpha (Reach=R13, Date=Jul-09) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -156.3133 181 .3645 Unequal 5.14 -156.3133 256.4882 Note: T-alpha (Equal) = 2.2281, T-alpha (Unequal) = 2.5500 Standard Error 104.7109 104.7109 95.0% LCL Difference -389.6237 -423.3279 95.0% UCL Difference 76.99702 110.7012 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.4928 0.166349 No Difference < 0 -1.4928 0.083175 No Difference > 0 -1.4928 0.916825 No Difference: (Reach=R13, Date=Jul-08)-(Reach=R13, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.271891 0.399975 0.001167 Power (Alpha=.010) 0.094263 0.152158 0.000134 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.4928 0.194171 No Difference < 0 -1.4928 0.097086 No Difference > 0 -1.4928 0.902914 No Difference: (Reach=R13, Date=Jul-08)-(Reach=R13, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.231969 0.363922 0.001492 Power (Alpha=.010) 0.067001 0.117241 0.000213 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R13, Date=Jul-08) Kurtosis Normality (Reach=R13, Date=Jul-08) Omnibus Normality (Reach=R13, Date=Jul-08) Skewness Normality (Reach=R13, Date=Jul-09) Kurtosis Normality (Reach=R13, Date=Jul-09) Omnibus Normality (Reach=R13, Date=Jul-09) Variance-Ratio Equal-Variance Test 72.6840 Modified-Levene Equal-Variance Test 1.0421 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.000230 0.331403 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-08 Reach=R13, Date=Jul-09 Count 6 6 Median 82.655 138.37 95.0% LCL of Median 59.5 116.85 95.0% UCL of Median 142.18 760 4/10/2013 10:42:20 AM 1970 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13, Date=Jul-08 Reach=R13, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 3 24 33 54 Multiplicity Factor = 0 Mean of W 39 39 Std Dev of W 6.244998 6.244998 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015152 Yes 0.007576 Yes 0.992424 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.4019 0.016309 Yes -2.4019 0.008155 Yes -2.4019 0.991845 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.3219 0.020241 Yes -2.3219 0.010120 Yes -2.4820 0.993467 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.833333 0.833333 0.000000 Reject H0 if Greater Than 0.6980 0.6980 0.6980 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0260 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:42:20 AM 1971 4/10/2013 10:42:20 AM 1972 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R13, Date=Jul-08 6 86.36833 29.88 12.19846 55.01119 117.7255 Reach=R13, Date=Nov-12 5 77.02 12.92892 5.781989 60.96663 93.07338 Note: T-alpha (Reach=R13, Date=Jul-08) = 2.5706, T-alpha (Reach=R13, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 9.348333 23.88096 Unequal 7.05 9.348333 32.55721 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 2.3610 Standard Error 14.46064 13.4994 95.0% LCL Difference -23.3639 -22.52321 95.0% UCL Difference 42.06057 41.21988 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6465 0.534113 No Difference < 0 0.6465 0.732943 No Difference > 0 0.6465 0.267057 No Difference: (Reach=R13, Date=Jul-08)-(Reach=R13, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.089372 0.012366 0.147790 Power (Alpha=.010) 0.021752 0.001942 0.038394 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6925 0.510770 No Difference < 0 0.6925 0.744615 No Difference > 0 0.6925 0.255385 No Difference: (Reach=R13, Date=Jul-08)-(Reach=R13, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.092592 0.011422 0.154583 Power (Alpha=.010) 0.022270 0.001827 0.039737 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R13, Date=Jul-08) Kurtosis Normality (Reach=R13, Date=Jul-08) Omnibus Normality (Reach=R13, Date=Jul-08) Skewness Normality (Reach=R13, Date=Nov-12) Kurtosis Normality (Reach=R13, Date=Nov-12) Omnibus Normality (Reach=R13, Date=Nov-12) Variance-Ratio Equal-Variance Test 5.3412 Modified-Levene Equal-Variance Test 1.1303 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.129624 0.315406 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-08 Reach=R13, Date=Nov-12 Count 6 5 Median 82.655 71 95.0% LCL of Median 59.5 95.0% UCL of Median 142.18 4/10/2013 10:42:20 AM 1973 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13, Date=Jul-08 Reach=R13, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 16 37 14 29 Multiplicity Factor = 0 Mean of W 36 30 Std Dev of W 5.477226 5.477226 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.930736 No 0.534632 No 0.465368 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.1826 0.855132 No -0.1826 0.572434 No -0.1826 0.427566 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.0913 0.927264 No -0.2739 0.607904 No -0.0913 0.463632 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.333333 0.333333 0.300000 Reject H0 if Greater Than 0.7246 0.7246 0.7246 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8182 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:42:20 AM 1974 4/10/2013 10:42:21 AM 1981 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R13, Date=Jul-09 6 242.6817 254.7418 103.9979 -24.65345 510.0168 Reach=R13, Date=Nov-12 5 77.02 12.92892 5.781989 60.96663 93.07338 Note: T-alpha (Reach=R13, Date=Jul-09) = 2.5706, T-alpha (Reach=R13, Date=Nov-12) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 165.6617 190.0688 Unequal 5.03 165.6617 255.0697 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 2.5658 Standard Error 115.0924 104.1585 95.0% LCL Difference -94.69544 -101.5925 95.0% UCL Difference 426.0188 432.9158 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.4394 0.183901 No Difference < 0 1.4394 0.908049 No Difference > 0 1.4394 0.091951 No Difference: (Reach=R13, Date=Jul-09)-(Reach=R13, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.251570 0.001419 0.376808 Power (Alpha=.010) 0.083465 0.000171 0.137238 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.5905 0.172243 No Difference < 0 1.5905 0.913879 No Difference > 0 1.5905 0.086121 No Difference: (Reach=R13, Date=Jul-09)-(Reach=R13, Date=Nov-12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.254665 0.001129 0.394167 Power (Alpha=.010) 0.075150 0.000160 0.130611 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R13, Date=Jul-09) Kurtosis Normality (Reach=R13, Date=Jul-09) Omnibus Normality (Reach=R13, Date=Jul-09) Skewness Normality (Reach=R13, Date=Nov-12) Kurtosis Normality (Reach=R13, Date=Nov-12) Omnibus Normality (Reach=R13, Date=Nov-12) Variance-Ratio Equal-Variance Test 388.2181 Modified-Levene Equal-Variance Test 1.0554 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.000037 0.331090 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-09 Reach=R13, Date=Nov-12 Count 6 5 Median 138.37 71 95.0% LCL of Median 116.85 95.0% UCL of Median 760 4/10/2013 10:42:21 AM 1982 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13, Date=Jul-09 Reach=R13, Date=Nov-12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 30 51 0 15 Multiplicity Factor = 0 Mean of W 36 30 Std Dev of W 5.477226 5.477226 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.004329 Yes 0.997835 No 0.002165 Yes Approx. Without Correction Prob Z-Value Level Reject H0 at .050 -2.7386 0.006170 Yes -2.7386 0.996915 No -2.7386 0.003085 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.6473 0.008113 Yes -2.8299 0.997672 No -2.6473 0.004057 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7246 0.7246 0.7246 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0043 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:42:21 AM 1983 APPENDIX F-2 LARGEMOUTH BASS STATISTICS SPATIAL EVALUATION 4/10/2013 10:39:10 AM 451 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R02, Date=Jun-02 7 2.5 0 0 2.5 2.5 Reach=R06, Date=Jun-02 4 163 260.0933 130.0466 -250.8664 576.8664 Note: T-alpha (Reach=R02, Date=Jun-02) = 0.0000, T-alpha (Reach=R06, Date=Jun-02) = 3.1824 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 -160.5 150.1649 Unequal 3.00 -160.5 260.0933 Note: T-alpha (Equal) = 2.2622, T-alpha (Unequal) = 3.1824 Standard Error 94.12083 130.0466 95.0% LCL Difference -373.4161 -574.3664 95.0% UCL Difference 52.41613 253.3664 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.7053 0.122338 No Difference < 0 -1.7053 0.061169 No Difference > 0 -1.7053 0.938831 No Difference: (Reach=R02, Date=Jun-02)-(Reach=R06, Date=Jun-02) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.332080 0.472492 0.000612 Power (Alpha=.010) 0.121997 0.192881 0.000068 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.2342 0.305004 No Difference < 0 -1.2342 0.152502 No Difference > 0 -1.2342 0.847498 No Difference: (Reach=R02, Date=Jun-02)-(Reach=R06, Date=Jun-02) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.143221 0.249715 0.003875 Power (Alpha=.010) 0.033008 0.062508 0.000674 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R02, Date=Jun-02) Kurtosis Normality (Reach=R02, Date=Jun-02) Omnibus Normality (Reach=R02, Date=Jun-02) Skewness Normality (Reach=R06, Date=Jun-02) Kurtosis Normality (Reach=R06, Date=Jun-02) Omnibus Normality (Reach=R06, Date=Jun-02) Variance-Ratio Equal-Variance Test 0.0000 Modified-Levene Equal-Variance Test 3.9381 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.000000 0.078495 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02, Date=Jun-02 Reach=R06, Date=Jun-02 Count 7 4 Median 2.5 51.25 95.0% LCL of Median 2.5 95.0% UCL of Median 2.5 4/10/2013 10:39:10 AM 452 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02, Date=Jun-02 Reach=R06, Date=Jun-02 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 7 35 21 31 Multiplicity Factor= 720 Mean of W 42 24 Std Dev of W 3.56753 3.56753 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Approx. With Correction Prob Reject H0 Prob Reject H0 Z-Value Level at .050 Z-Value Level at .050 1.9621 0.049746 Yes 1.8220 0.068457 No 1.9621 0.024873 Yes 1.8220 0.034228 Yes 1.9621 0.975127 No 2.1023 0.982236 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.500000 0.500000 0.000000 Reject H0 if Greater Than 0.7452 0.7452 0.7452 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.4152 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:39:10 AM 453 4/10/2013 10:41:07 AM 1441 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R06, Date=Jun-02 4 163 260.0933 130.0466 -250.8664 576.8664 Reach=R09, Date=Jun-02 6 553.5 231.1075 94.34927 310.9675 796.0325 Note: T-alpha (Reach=R06, Date=Jun-02) = 3.1824, T-alpha (Reach=R09, Date=Jun-02) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -390.5 242.3837 Unequal 5.99 -390.5 347.9356 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4476 Standard Error 156.458 160.6671 95.0% LCL Difference -751.2928 -783.7486 95.0% UCL Difference -29.70714 2.748622 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.4959 0.037180 Yes Difference < 0 -2.4959 0.018590 Yes Difference > 0 -2.4959 0.981410 No Difference: (Reach=R06, Date=Jun-02)-(Reach=R09, Date=Jun-02) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.591690 0.736491 0.000038 Power (Alpha=.010) 0.282437 0.403016 0.000004 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.4305 0.051175 No Difference < 0 -2.4305 0.025588 Yes Difference > 0 -2.4305 0.974412 No Difference: (Reach=R06, Date=Jun-02)-(Reach=R09, Date=Jun-02) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.530962 0.691686 0.000060 Power (Alpha=.010) 0.220631 0.335502 0.000007 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R06, Date=Jun-02) Kurtosis Normality (Reach=R06, Date=Jun-02) Omnibus Normality (Reach=R06, Date=Jun-02) Skewness Normality (Reach=R09, Date=Jun-02) Kurtosis Normality (Reach=R09, Date=Jun-02) Omnibus Normality (Reach=R09, Date=Jun-02) Variance-Ratio Equal-Variance Test 1.2666 Modified-Levene Equal-Variance Test 0.0001 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.760506 0.993797 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06, Date=Jun-02 Reach=R09, Date=Jun-02 Count 4 6 Median 51.25 629 95.0% LCL 95.0% UCL of Median of Median 152 788 4/10/2013 10:41:07 AM 1442 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06, Date=Jun-02 Reach=R09, Date=Jun-02 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 2 12 22 43 Multiplicity Factor = 6 Mean of W 22 33 Std Dev of W 4.676181 4.676181 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.1385 0.032476 Yes -2.1385 0.016238 Yes -2.1385 0.983762 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.0316 0.042197 Yes -2.0316 0.021098 Yes -2.2454 0.987629 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.750000 0.750000 0.000000 Reject H0 if Greater Than 0.7612 0.7612 0.7612 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.0952 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:07 AM 1443 4/10/2013 10:39:17 AM 523 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R02, Date=Nov-04 5 103.68 24.48207 10.94872 73.28149 134.0785 Reach=R05, Date=Nov-04 10 3897.8 3255.094 1029.351 1569.246 6226.354 Note: T-alpha (Reach=R02, Date=Nov-04) = 2.7764, T-alpha (Reach=R05, Date=Nov-04) = 2.2622 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 -3794.12 2708.436 Unequal 9.00 -3794.12 3255.187 Note: T-alpha (Equal) = 2.1604, T-alpha (Unequal) = 2.2621 Standard Error 1483.472 1029.409 95.0% LCL Difference -6998.966 -6122.726 95.0% UCL Difference -589.2744 -1465.514 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.5576 0.023847 Yes Difference < 0 -2.5576 0.011924 Yes Difference > 0 -2.5576 0.988076 No II ZJ o en D CD ilCD O o Z Difference: (Reach=R02, Date= Nov-04)-(Reach The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.657590 0.781200 0.000023 Power (Alpha=.010) 0.367278 0.486316 0.000002 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.6857 0.005029 Yes Difference < 0 -3.6857 0.002514 Yes Difference > 0 -3.6857 0.997486 No Difference: (Reach=R02, Date=Nov-04)-(Reach=R05, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.905241 0.959559 0.000000 Power (Alpha=.010) 0.665112 0.785264 0.000000 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R02, Date=Nov-04) Kurtosis Normality (Reach=R02, Date=Nov-04) Omnibus Normality (Reach=R02, Date=Nov-04) Skewness Normality (Reach=R05, Date=Nov-04) Kurtosis Normality (Reach=R05, Date=Nov-04) Omnibus Normality (Reach=R05, Date=Nov-04) Variance-Ratio Equal-Variance Test 17677.9021 Modified-Levene Equal-Variance Test 11.5340 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 1.0731 -0.5109 1.4125 0.000000 0.004777 0.283239 Cannot reject normality 0.609424 Cannot reject normality 0.493493 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R02, Date=Nov-04 Reach=R05, Date=Nov-04 Count 5 10 Median 114 3355 95.0% LCL 95.0% UCL of Median of Median 720 8960 4/10/2013 10:39:17 AM 524 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02, Date=Nov-04 Reach=R05, Date=Nov-04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 50 105 Multiplicity Factor = 0 Mean of W 40 80 Std Dev of W 8.164966 8.164966 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000666 Yes 0.000333 Yes 0.999667 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.0619 0.002200 Yes -3.0619 0.001100 Yes -3.0619 0.998900 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.0006 0.002694 Yes -3.0006 0.001347 Yes -3.1231 0.999105 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.6698 0.6698 0.6698 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0007 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:39:17 AM 525 4/10/2013 10:40:07 AM 967 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Nov-04 10 3897.8 3255.094 1029.351 1569.246 6226.354 Reach=R06, Date=Nov-04 5 68.72 23.02981 10.29924 40.12472 97.31528 Note: T-alpha (Reach=R05, Date=Nov-04) = 2.2622, T-alpha (Reach=R06, Date=Nov-04) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 3829.08 2708.432 Unequal 9.00 3829.08 3255.176 Note: T-alpha (Equal) = 2.1604, T-alpha (Unequal) = 2.2621 Standard Error 1483.469 1029.403 95.0% LCL Difference 624.239 1500.48 95.0% UCL Difference 7033.921 6157.68 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.5812 0.022806 Yes Difference < 0 2.5812 0.988597 No Difference > 0 2.5812 0.011403 Yes Difference: (Reach=R05, Date=Nov-04)-(Reach=R06, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.665526 0.000020 0.787710 Power (Alpha=.010) 0.374990 0.000001 0.494676 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.7197 0.004771 Yes Difference < 0 3.7197 0.997614 No Difference > 0 3.7197 0.002386 Yes Difference: (Reach=R05, Date=Nov-04)-(Reach=R06, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.910161 0.000000 0.962181 Power (Alpha=.010) 0.674848 0.000000 0.793376 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Nov-04) Kurtosis Normality (Reach=R05, Date=Nov-04) Omnibus Normality (Reach=R05, Date=Nov-04) Skewness Normality (Reach=R06, Date=Nov-04) Kurtosis Normality (Reach=R06, Date=Nov-04) Omnibus Normality (Reach=R06, Date=Nov-04) Variance-Ratio Equal-Variance Test 19977.7505 Modified-Levene Equal-Variance Test 11.5391 Probability 1.0731 -0.5109 1.4125 0.0000 Decision(.050) 0.283239 Cannot reject normality 0.609424 Cannot reject normality 0.493493 Cannot reject normality 1.000000 Cannot reject normality 0.000000 0.004770 Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R06, Date=Nov-04 Count 10 5 Median 3355 72.4 95.0% LCL of Median 720 95.0% UCL of Median 8960 4/10/2013 10:40:07 AM 968 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Nov-04 Reach=R06, Date=Nov-04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 50 105 0 15 Multiplicity Factor = 0 Mean of W 80 40 Std Dev of W 8.164966 8.164966 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000666 Yes 0.999667 No 0.000333 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.0619 0.002200 Yes -3.0619 0.998900 No -3.0619 0.001100 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.0006 0.002694 Yes -3.1231 0.999105 No -3.0006 0.001347 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.6698 0.6698 0.6698 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0007 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:07 AM 969 Normal Probability Plot of Largernouth_Bass_PFOS_ppb when Reach=R05, Date=Nov Normal Probability 3lot of Largemouth_Ba5s_PFQS_ppb when Reach=R0B, Date=N Largemouth_Bass_PFOS_ppbvs Groups Percent ofValues 4/10/2013 10:41:15 AM 1498 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R06, Date=Nov-04 5 68.72 23.02981 10.29924 40.12472 97.31528 Reach=R09, Date=Nov-04 5 477.6 178.4959 79.82581 255.968 Note: T-alpha (Reach=R06, Date=Nov-04) = 2.7764, T-alpha (Reach=R09, Date=Nov-04) = 2.7764 699.232 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -408.88 127.2619 Unequal 4.13 -408.88 179.9755 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7415 Standard Error 80.48748 80.48748 95.0% LCL Difference -594.4844 -629.5386 95.0% UCL Difference -223.2755 -188.2214 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.0800 0.000953 Yes Difference < 0 -5.0800 0.000477 Yes Difference > 0 -5.0800 0.999523 No Difference: (Reach=R06, Date=Nov-04)-(Reach=R09, Date: Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.992821 0.998442 0.000000 Power (Alpha=.010) 0.919025 0.966419 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.0800 0.006486 Yes Difference < 0 -5.0800 0.003243 Yes Difference > 0 -5.0800 0.996757 No Difference: (Reach=R06, Date=Nov-04)-(Reach=R09, Date: Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.963388 0.992987 0.000000 Power (Alpha=.010) 0.687869 0.844081 0.000000 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R06, Date= Nov-04) Kurtosis Normality (Reach=R06, Date=Nov-04) Omnibus Normality (Reach=R06, Date=Nov-04) Skewness Normality (Reach=R09, Date= Nov-04) Kurtosis Normality (Reach=R09, Date=Nov-04) Omnibus Normality (Reach=R09, Date=Nov-04) Variance-Ratio Equal-Variance Test 60.0726 Modified-Levene Equal-Variance Test 5.0848 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.001591 0.054147 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06, Date=Nov-04 Reach=R09, Date=Nov-04 Count 5 5 Median 72.4 432 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:41:15 AM 1499 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06, Date=Nov-04 Reach=R09, Date=Nov-04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Z-Value Level Reject H0 at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:15 AM 1500 Largernouth_Bass_PFOS_ppbvs Grouas 750n o J------------------- 1--------------------------------------- 1------------ Reach-ROB, Cat3=Ncv-04 Reach=R09, Date-Nov-C4 Grouas 4/10/2013 10:41:48 AM 1735 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R09, Date=Nov-04 5 477.6 178.4959 79.82581 255.968 Reach=R11, Date=Nov-04 5 356.28 351.4388 157.1682 -80.08884 792.6489 Note: T-alpha (Reach=R09, Date=Nov-04) = 2.7764, T-alpha (R each=R 11, Date=Nov-04) = 2.7764 699.232 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 121.32 278.7203 Unequal 5.93 121.32 394.17 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4534 Standard Error 176.2782 176.2782 95.0% LCL Difference -285.1782 -311.1659 95.0% UCL Difference 527.8182 553.8059 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6882 0.510767 No Difference < 0 0.6882 0.744616 No Difference > 0 0.6882 0.255384 No Difference: (Reach=R09, D ate=N ov-04)-(R each=R 11, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.093494 0.011339 0.155415 Power (Alpha=.010) 0.022819 0.001783 0.040526 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6882 0.517290 No Difference < 0 0.6882 0.741355 No Difference > 0 0.6882 0.258645 No Difference: (Reach=R09, D ate=N ov-04)-(R each=R 11, Date=Nov-04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.089878 0.011853 0.150945 Power (Alpha=.010) 0.021077 0.001949 0.037818 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R09, Date=Nov-04) Kurtosis Normality (Reach=R09, Date=Nov-04) Omnibus Normality (Reach=R09, Date=Nov-04) Skewness Normality (Reach=R11, Date=Nov-04) Kurtosis Normality (Reach=R11, Date=Nov-04) Omnibus Normality (Reach=R11, Date=Nov-04) Variance-Ratio Equal-Variance Test 3.8765 Modified-Levene Equal-Variance Test 0.7228 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.217815 0.419951 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09, Date=Nov-04 Reach=R11, Date=Nov-04 Count 5 5 Median 432 147 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:41:48 AM 1736 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09, Date=Nov-04 Reach=R11, Date=Nov-04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 16 31 9 24 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.726191 No 0.273810 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.7311 0.464702 No 0.7311 0.767649 No 0.7311 0.232351 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.6267 0.530869 No 0.8356 0.798302 No 0.6267 0.265435 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.200000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:48 AM 1737 Norrral Probability' Piet of Largemou:h_Bass_PFOS_ppb when Reaoh=R09, Date=N Normal Prooability Plot of Larcemouth_Bass_P-0_ppb when Reach=R11. Date=N 1 900 n 10 25 50 75 Percent ofValues 90 95 Largernouth_Bass_PFOS_ppbvs Grouos 39 750 5 10 25 50 75 Percent ofValies 90 95 99 o J------------------- 1--------------------------------------- 1------------ Reach-R09, 0at3=Ncv-04 Peach=R' 1, Date-Nov-C4 Grouos 4/10/2013 10:39:27 AM 616 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R02, Date=Dec-06 5 34.24 24.16574 10.80725 4.234271 64.24573 Reach=R05, Date=Dec-06 10 2726 2687.829 849.9661 803.2431 4648.757 Note: T-alpha (Reach=R02, Date=D ec-06) = 2.7764, T-alpha (Reach=R05, Date=D ec-06) = 2.2622 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 -2691.76 2236.449 Unequal 9.00 -2691.76 2687.938 Note: T-alpha (Equal) = 2.1604, T-alpha (Unequal) = 2.2620 Standard Error 1224.953 850.0348 95.0% LCL Difference -5338.111 -4614.578 95.0% UCL Difference -45.40879 -768.9424 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.1974 0.046717 Yes Difference < 0 -2.1974 0.023358 Yes Difference > 0 -2.1974 0.976642 No II ZJ o en D CD ilCD Difference: (Reach=R02, Date= Dec-06)-(Reach Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.529553 0.668637 0.000093 Power (Alpha=.010) 0.257421 0.361311 0.000008 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.1666 0.011423 Yes Difference < 0 -3.1666 0.005711 Yes Difference > 0 -3.1666 0.994289 No Difference: (Reach=R02, Date=Dec-06)-(Reach=R05, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.804192 0.898244 0.000002 Power (Alpha=.010) 0.505568 0.640367 0.000000 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R02, Date=Dec-06) Kurtosis Normality (Reach=R02, D ate=Dec-06) Omnibus Normality (Reach=R02, Date=Dec-06) Skewness Normality (Reach=R05, Date=Dec-06) Kurtosis Normality (Reach=R05, D ate=Dec-06) Omnibus Normality (Reach=R05, Date=Dec-06) Variance-Ratio Equal-Variance Test 12370.9488 Modified-Levene Equal-Variance Test 3.9245 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 1.0392 -1.0975 2.2845 0.000000 0.069147 0.298698 Cannot reject normality 0.272432 Cannot reject normality 0.319107 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02, Date=Dec-06 Reach=R05, Date=Dec-06 Count 5 10 Median 19.6 1127.5 95.0% LCL 95.0% UCL of Median of Median 300 5310 4/10/2013 10:39:27 AM 617 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02, Date=Dec-06 Reach=R05, Date=Dec-06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 50 105 Multiplicity Factor = 0 Mean of W 40 80 Std Dev of W 8.164966 8.164966 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000666 Yes 0.000333 Yes 0.999667 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.0619 0.002200 Yes -3.0619 0.001100 Yes -3.0619 0.998900 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.0006 0.002694 Yes -3.0006 0.001347 Yes -3.1231 0.999105 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.6698 0.6698 0.6698 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0007 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Nomai Probability Plot of _arge-nouth_Eas3_PFOS_ppb when Reach~RC2, Jate~ 4/10/2013 10:39:27 AM 618 8000 -, Largemouth_Bass_PFOS_ppbvs Groups 4/10/2013 10:40:19 AM 1060 Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Dec-06 10 2726 2687.829 849.9661 803.2431 4648.757 Reach=R09, Date=Dec-06 5 304.6 63.22025 28.27295 226.1017 383.0983 Note: T-alpha (Reach:=R05, Date=D ec-06) = 2.2622, T-alpha (Reach=R09, Date=D ec-06) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 2421.4 2236.684 Unequal 9.02 2421.4 2688.572 Note: T-alpha (Equal) = 2.1604, T-alpha (Unequal) = 2.2614 Standard Error 1225.082 850.4362 95.0% LCL Difference -225.229 498.2265 95.0% UCL Difference 5068.029 4344.573 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.9765 0.069706 No Difference < 0 1.9765 0.965147 No Difference > 0 1.9765 0.034853 Yes Difference: (Reach=R05, Date= Dec-06)-(Reach =R09, Date= Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.448483 0.000211 0.589944 Power (Alpha=.010) 0.199538 0.000019 0.290480 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.8472 0.019134 Yes Difference < 0 2.8472 0.990433 No Difference > 0 2.8472 0.009567 Yes Difference: (Reach=R05, Date=Dec-06)-(Reach=R09, Date=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.717772 0.000008 0.836412 Power (Alpha=.010) 0.404945 0.000001 0.537415 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Dec-06) Kurtosis Normality (Reach=R05, D ate=Dec-06) Omnibus Normality (Reach=R05, Date=Dec-06) Skewness Normality (Reach=R09, Date=Dec-06) Kurtosis Normality (Reach=R09, D ate=Dec-06) Omnibus Normality (Reach=R09, Date=Dec-06) Variance-Ratio Equal-Variance Test 1807.5520 Modified-Levene Equal-Variance Test 3.8227 Probability 1.0392 -1.0975 2.2845 0.0000 Decision(.050) 0.298698 Cannot reject normality 0.272432 Cannot reject normality 0.319107 Cannot reject normality 1.000000 Cannot reject normality 0.000001 0.072409 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R09, Date=Dec-06 Count 10 5 Median 1127.5 296 95.0% LCL of Median 300 95.0% UCL of Median 5310 4/10/2013 10:40:19 AM 1061 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Dec-06 Reach=R09, Date=Dec-06 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 43.5 98.5 6.5 21.5 Multiplicity Factor = 6 Mean of W 80 40 Std Dev of W 8.157672 8.157672 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Approx. With Correction Prob Reject H0 Prob Reject H0 Z-Value Level at .050 Z-Value Level at .050 -2.2678 0.023341 Yes -2.2065 0.027348 Yes -2.2678 0.988329 No -2.3291 0.990073 No -2.2678 0.011671 Yes -2.2065 0.013674 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.6698 0.6698 0.6698 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0193 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:19 AM Normal Probability Plot of Largemouth_Bass_PFOS_ppb when Reach=RD5, Date=De 1062 3000 -, Largemouth_Bass_PFOS_ppbvs Groups 4/10/2013 10:41:53 AM 1774 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R09, Date=Dec-06 5 304.6 63.22025 28.27295 226.1017 383.0983 Reach=R11, Date=Dec-06 5 298.4 39.52594 17.67654 249.3221 347.4779 Note: T-alpha (Reach=R09, Date=D ec-06) = 2.7764, T-alpha (R each=R 11, Date=D ec-06) = 2.7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 6.2 52.72144 Unequal 6.71 6.2 74.55937 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3853 Standard Error 33.34396 33.34396 95.0% LCL Difference -70.69132 -73.3354 95.0% UCL Difference 83.09132 85.7354 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.1859 0.857119 No Difference < 0 0.1859 0.571440 No Difference > 0 0.1859 0.428560 No Difference: (Reach=R09, D ate=D ec-06)-(R each=R 11, D ate=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.053117 0.034695 0.070238 Power (Alpha=.010) 0.010867 0.006485 0.015058 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.1859 0.857985 No Difference < 0 0.1859 0.571008 No Difference > 0 0.1859 0.428992 No Difference: (Reach=R09, D ate=D ec-06)-(R each=R 11, D ate=Dec-06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.052976 0.034899 0.069871 Power (Alpha=.010) 0.010805 0.006568 0.014882 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R09, Date=Dec-06) Kurtosis Normality (Reach=R09, D ate=Dec-06) Omnibus Normality (Reach=R09, Date=Dec-06) Skewness Normality (Reach=R11, Date=Dec-06) Kurtosis Normality (Reach=R11, D ate=Dec-06) Omnibus Normality (Reach=R11, Date=Dec-06) Variance-Ratio Equal-Variance Test 2.5583 Modified-Levene Equal-Variance Test 0.5934 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 0.385098 0.463250 Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09, Date=Dec-06 Reach=R11, Date=Dec-06 Count 5 5 Median 296 295 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 10:41:53 AM 1775 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09, Date=Dec-06 Reach=R11, Date=Dec-06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 13 28 12 27 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 1.000000 No 0.579365 No 0.420635 No Approx. Without Correction Prob Z-Value Level Reject H0 at .050 0.1044 0.916815 No 0.1044 0.541593 No 0.1044 0.458407 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.0000 1.000000 No 0.2089 0.582734 No 0.0000 0.500000 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.200000 0.200000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 1.0000 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:41:53 AM 1776 4/10/2013 10:38:31 AM 34 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R01, Date=Jul-07 6 72.18333 44.53688 18.1821 25.44474 118.9219 Reach=R05, Date=Jul-07 12 1281.408 1086.755 313.7191 590.9171 Note: T-alpha (R each=R 01, Date=Jul-07) = 2.5706, T-alpha (Reach=R05, Date =Jul-07) = 2.2010 1971.9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 -1209.225 901.4335 Unequal 11.07 -1209.225 1087.667 Note: T-alpha (Equal) = 2.1199, T-alpha (Unequal) = 2.1992 Standard Error 450.7168 314.2456 95.0% LCL Difference -2164.702 -1900.313 95.0% UCL Difference -253.7481 -518.1367 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.6829 0.016334 Yes Difference < 0 -2.6829 0.008167 Yes Difference > 0 -2.6829 0.991833 No Difference: (Reach=R01, Date=Jul-07)-(Reach= R05, Date=Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.712054 0.821721 0.000012 Power (Alpha=.010) 0.433045 0.551372 0.000001 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.8480 0.002676 Yes Difference < 0 -3.8480 0.001338 Yes Difference > 0 -3.8480 0.998662 No Difference: (Reach=R01, Date=Jul-07)-(Reach=R05, Date=Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.937935 0.974658 0.000000 Power (Alpha=.010) 0.753809 0.850000 0.000000 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R01, Date=Jul-07) Kurtosis Normality (Reach=R01, Date=Jul-07) Omnibus Normality (Reach=R01, Date=Jul-07) Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Variance-Ratio Equal-Variance Test 595.4204 Modified-Levene Equal-Variance Test 10.4101 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 1.2669 0.0239 1.6055 0.000001 0.005276 0.205206 Cannot reject normality 0.980959 Cannot reject normality 0.448095 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R01, Date=Jul-07 Reach=R05, Date=Jul-07 Count 6 12 Median 65.5 1090.65 95.0% LCL of Median 22.9 310.4 95.0% UCL of Median 145.7 2044 4/10/2013 10:38:31 AM 35 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01, Date=Jul-07 Reach=R05, Date=Jul-07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 21 72 150 Multiplicity Factor = 0 Mean of W 57 114 Std Dev of W 10.67708 10.67708 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000108 Yes 0.000054 Yes 0.999946 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.3717 0.000747 Yes -3.3717 0.000374 Yes -3.3717 0.999626 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.3249 0.000885 Yes -3.3249 0.000442 Yes -3.4185 0.999685 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.6202 0.6202 0.6202 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0001 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:38:31 AM 36 4/10/2013 10:40:31 AM 1153 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-07 12 1281.408 1086.755 313.7191 590.9171 Reach=R13, Date=Jul-07 6 148.85 56.32444 22.99436 89.74112 207.9589 Note: T-alpha (Reach=R05, Date=Jul-07) = 2.2010, T-alpha (Reach=R13, Date=Jul-07) = 2.5706 1971.9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 1132.558 901.6396 Unequal 11.12 1132.558 1088.214 Note: T-alpha (Equal) = 2.1199, T-alpha (Unequal) = 2.1981 Standard Error 450.8198 314.5607 95.0% LCL Difference 176.8631 441.1088 95.0% UCL Difference 2088.254 1824.008 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.5122 0.023099 Yes Difference < 0 2.5122 0.988451 No Difference > 0 2.5122 0.011549 Yes Difference: (Reach=R05, Date= Jul-07)-(Reach= R13, Date=Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.655284 0.000025 0.776061 Power (Alpha=.010) 0.374201 0.000002 0.489508 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.6004 0.004098 Yes Difference < 0 3.6004 0.997951 No Difference > 0 3.6004 0.002049 Yes Difference: (Reach=R05, Date=Jul-07)-(Reach=R13, Date=Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.905909 0.000000 0.957719 Power (Alpha=.010) 0.685335 0.000000 0.795302 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Skewness Normality (Reach=R13, Date=Jul-07) Kurtosis Normality (Reach=R13, Date=Jul-07) Omnibus Normality (Reach=R13, Date=Jul-07) Variance-Ratio Equal-Variance Test 372.2798 Modified-Levene Equal-Variance Test 10.3880 Probability 1.2669 0.0239 1.6055 0.0000 Decision(.050) 0.205206 Cannot reject normality 0.980959 Cannot reject normality 0.448095 Cannot reject normality 1.000000 Cannot reject normality 0.000003 0.005314 Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Jul-07 Reach=R13, Date=Jul-07 Count 12 6 Median 1090.65 139.6 95.0% LCL of Median 310.4 86.7 95.0% UCL of Median 2044 254.7 4/10/2013 10:40:31 AM 1154 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-07 Reach=R13, Date=Jul-07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 70 148 2 23 Multiplicity Factor = 0 Mean of W 114 57 Std Dev of W 10.67708 10.67708 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000431 Yes 0.999785 No 0.000215 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.1844 0.001451 Yes -3.1844 0.999275 No -3.1844 0.000725 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.1376 0.001704 Yes -3.2312 0.999384 No -3.1376 0.000852 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.833333 0.000000 0.833333 Reject H0 if Greater Than 0.6202 0.6202 0.6202 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0041 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:31 AM Normal Probability Plot of Largernouth_Bass_PFOS_ppbwhen Reach=R05, Date=Jul 1155 4000 -, Largemouth_Bass_PFOS_ppbvs Groups 4/10/2013 10:38:41 AM 142 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R01, Date=Jul-08 6 59.27833 20.29346 8.284771 37.98165 80.57502 Reach=R05, Date=Jul-08 12 1453.35 1467.149 423.5294 521.1681 2385.532 Note: T-alpha (Reach=R01, Date=Jul-08) = 2.5706, T-alpha (Reach=R05, Date=Jul-08) = 2.2010 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 -1394.072 1216.548 Unequal 11.01 -1394.072 1467.289 Note: T-alpha (Equal) = 2.1199, T-alpha (Unequal) = 2.2008 Standard Error 608.2742 423.6104 95.0% LCL Difference -2683.555 -2326.345 95.0% UCL Difference -104.5879 -461.7984 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.2918 0.035815 Yes Difference < 0 -2.2918 0.017907 Yes Difference > 0 -2.2918 0.982093 No Difference: (Reach= R01, Date=Jul-08)-(Reach= R05, Date= Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.576618 0.708309 0.000060 Power (Alpha=.010) 0.302450 0.410156 0.000004 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.2909 0.007186 Yes Difference < 0 -3.2909 0.003593 Yes Difference > 0 -3.2909 0.996407 No Difference: (Reach=R01, Date=Jul-08)-(Reach=R05, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.849354 0.924274 0.000001 Power (Alpha=.010) 0.586862 0.710057 0.000000 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R01, Date=Jul-08) Kurtosis Normality (Reach=R01, Date=Jul-08) Omnibus Normality (Reach=R01, Date=Jul-08) Skewness Normality (Reach=R05, Date=Jul-08) Kurtosis Normality (Reach=R05, Date=Jul-08) Omnibus Normality (Reach=R05, Date=Jul-08) Variance-Ratio Equal-Variance Test 5226.8018 Modified-Levene Equal-Variance Test 5.4389 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 2.4107 1.9157 9.4811 0.000000 0.033083 0.015924 Reject normality 0.055405 Cannot reject normality 0.008734 Reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R01, Date=Jul-08 Reach=R05, Date=Jul-08 Count 6 12 Median 59.105 929.66 95.0% LCL of Median 26.38 337.38 95.0% UCL of Median 81.38 2253.98 4/10/2013 10:38:41 AM 143 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01, Date=Jul-08 Reach=R05, Date=Jul-08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 21 72 150 Multiplicity Factor = 0 Mean of W 57 114 Std Dev of W 10.67708 10.67708 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000108 Yes 0.000054 Yes 0.999946 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.3717 0.000747 Yes -3.3717 0.000374 Yes -3.3717 0.999626 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.3249 0.000885 Yes -3.3249 0.000442 Yes -3.4185 0.999685 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.6202 0.6202 0.6202 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0001 Plots Section 50 -||------------- Histogram 40 - I 30 n I| 20- ------------- 10- Largemojth_Bass_FFOS_ppb when Reach=ROI, Date=Jul-oe o -4------------------------------ --------------- ---------------1--------------- --------------- 0 1000 1000 3000 4300 5000 6000 Largemouth_Bass_RFOS_piob when Reach=R05, Date=Jul-oe Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Nomai Probability Plot of _arge-nouth_Eas3_PFOS_ppb when Reach~RC1, Jate~ 4/10/2013 10:38:41 AM 144 1 5 10 25 50 75 90 95 99 Percent ofValues 4/10/2013 10:40:39 AM 1225 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-08 12 1453.35 1467.149 423.5294 521.1681 2385.532 Reach=R13, Date=Jul-08 6 86.36833 29.88 12.19846 55.01119 117.7255 Note: T-alpha (Reach=R05, Date=Jul-08) = 2.2010, T-alpha (Reach=R13, Date=Jul-08) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 1366.982 1216.61 Unequal 11.02 1366.982 1467.453 Note: T-alpha (Equal) = 2.1199, T-alpha (Unequal) = 2.2005 Standard Error 608.3051 423.705 95.0% LCL Difference 77.43246 434.6016 95.0% UCL Difference 2656.531 2299.362 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2472 0.039087 Yes Difference < 0 2.2472 0.980457 No Difference > 0 2.2472 0.019543 Yes Difference: (Reach= R05, Date=Jul-08)-(Reach= R13, Date= Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.560180 0.000072 0.693498 Power (Alpha=.010) 0.288685 0.000005 0.394404 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.2263 0.008052 Yes Difference < 0 3.2263 0.995974 No Difference > 0 3.2263 0.004026 Yes Difference: (Reach=R05, Date=Jul-08)-(Reach=R13, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.835364 0.000001 0.915341 Power (Alpha=.010) 0.565886 0.000000 0.690776 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-08) Kurtosis Normality (Reach=R05, Date=Jul-08) Omnibus Normality (Reach=R05, Date=Jul-08) Skewness Normality (Reach=R13, Date=Jul-08) Kurtosis Normality (Reach=R13, Date=Jul-08) Omnibus Normality (Reach=R13, Date=Jul-08) Variance-Ratio Equal-Variance Test 2410.9432 Modified-Levene Equal-Variance Test 5.3883 Probability 2.4107 1.9157 9.4811 0.0000 Decision(.050) 0.015924 Reject normality 0.055405 Cannot reject normality 0.008734 Reject normality 1.000000 Cannot reject normality 0.000000 0.033800 Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Jul-08 Reach=R13, Date=Jul-08 Count 12 6 Median 929.66 82.655 95.0% LCL of Median 337.38 59.5 95.0% UCL of Median 2253.98 142.18 4/10/2013 10:40:39 AM 1226 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-08 Reach=R13, Date=Jul-08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 71 149 1 22 Multiplicity Factor = 0 Mean of W 114 57 Std Dev of W 10.67708 10.67708 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000215 Yes 0.999892 No 0.000108 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.2781 0.001045 Yes -3.2781 0.999477 No -3.2781 0.000523 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.2312 0.001233 Yes -3.3249 0.999558 No -3.2312 0.000616 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.916667 0.000000 0.916667 Reject H0 if Greater Than 0.6202 0.6202 0.6202 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0008 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:39 AM 1227 4/10/2013 10:38:50 AM 247 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R01, Date=Jul-09 6 53.44333 27.72979 11.32064 24.3427 82.54396 Reach=R05, Date=Jul-09 12 1011.468 1023.998 295.6027 360.8504 1662.085 Note: T-alpha (Reach=R01, Date=Jul-09) = 2.5706, T-alpha (Reach=R05, Date=Jul-09) = 2.2010 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 -958.0242 849.1955 Unequal 11.03 -958.0242 1024.373 Note: T-alpha (Equal) = 2.1199, T-alpha (Unequal) = 2.2002 Standard Error 424.5977 295.8194 95.0% LCL Difference -1858.131 -1608.886 95.0% UCL Difference -57.91713 -307.1622 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.2563 0.038397 Yes Difference < 0 -2.2563 0.019199 Yes Difference > 0 -2.2563 0.980801 No Difference: (Reach= R01, Date=Jul-09)-(Reach= R05, Date= Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.563544 0.696548 0.000069 Power (Alpha=.010) 0.291471 0.397606 0.000005 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.2385 0.007866 Yes Difference < 0 -3.2385 0.003933 Yes Difference > 0 -3.2385 0.996067 No Difference: (Reach=R01, Date=Jul-09)-(Reach=R05, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.838191 0.917144 0.000001 Power (Alpha=.010) 0.570157 0.694686 0.000000 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R01, Date=Jul-09) Kurtosis Normality (Reach=R01, Date=Jul-09) Omnibus Normality (Reach=R01, Date=Jul-09) Skewness Normality (Reach=R05, Date=Jul-09) Kurtosis Normality (Reach=R05, Date=Jul-09) Omnibus Normality (Reach=R05, Date=Jul-09) Variance-Ratio Equal-Variance Test 1363.6558 Modified-Levene Equal-Variance Test 4.4863 Probability 0.0000 Decision(.050) 1.000000 Cannot reject normality 2.0222 0.6651 4.5318 0.000000 0.050174 0.043152 Reject normality 0.505989 Cannot reject normality 0.103737 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-09 Reach=R05, Date=Jul-09 Count 6 12 Median 48.465 680.81 95.0% LCL of Median 19.85 230.63 95.0% UCL of Median 89.13 1987.94 4/10/2013 10:38:50 AM 248 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01, Date=Jul-09 Reach=R05, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 21 72 150 Multiplicity Factor = 0 Mean of W 57 114 Std Dev of W 10.67708 10.67708 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000108 Yes 0.000054 Yes 0.999946 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.3717 0.000747 Yes -3.3717 0.000374 Yes -3.3717 0.999626 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.3249 0.000885 Yes -3.3249 0.000442 Yes -3.4185 0.999685 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.6202 0.6202 0.6202 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0001 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Nomai Probability Plot of _arge-nouth_Eas3_PFOS_ppb when Reach~RC1, Jate~ 4/10/2013 10:38:50 AM 249 1 5 10 25 50 75 90 95 99 Percent ofValues Largemouth_Bass_PFOS_ppbvs Groups Groups 4/10/2013 10:40:48 AM 1294 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-09 12 1011.468 1023.998 295.6027 360.8504 1662.085 Reach=R13, Date=Jul-09 6 242.6817 254.7418 103.9979 -24.65345 510.0168 Note: T-alpha (Reach=R05, Date=Jul-09) = 2.2010, T-alpha (Reach=R13, Date =Jul-09) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 768.7858 860.9134 Unequal 13.44 768.7858 1055.208 Note: T-alpha (Equal) = 2.1199, T-alpha (Unequal) = 2.1532 Standard Error 430.4567 313.3632 95.0% LCL Difference -143.7416 94.04507 95.0% UCL Difference 1681.313 1443.527 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.7860 0.093067 No Difference < 0 1.7860 0.953467 No Difference > 0 1.7860 0.046533 Yes Difference: (Reach=R05, Date= Jul-09)-(Reach= R13, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.389480 0.000391 0.525900 Power (Alpha=.010) 0.166141 0.000035 0.245459 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.4533 0.028496 Yes Difference < 0 2.4533 0.985752 No Difference > 0 2.4533 0.014248 Yes Difference: (Reach=R05, Date=Jul-09)-(Reach=R13, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.623968 0.000034 0.752435 Power (Alpha=.010) 0.337364 0.000003 0.452682 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-09) Kurtosis Normality (Reach=R05, Date=Jul-09) Omnibus Normality (Reach=R05, Date=Jul-09) Skewness Normality (Reach=R13, Date=Jul-09) Kurtosis Normality (Reach=R13, Date=Jul-09) Omnibus Normality (Reach=R13, Date=Jul-09) Variance-Ratio Equal-Variance Test 16.1584 Modified-Levene Equal-Variance Test 3.1315 Probability 2.0222 0.6651 4.5318 0.0000 Decision(.050) 0.043152 Reject normality 0.505989 Cannot reject normality 0.103737 Cannot reject normality 1.000000 Cannot reject normality 0.006568 0.095843 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-09 Reach=R13, Date=Jul-09 Count 12 6 Median 680.81 138.37 95.0% LCL of Median 230.63 116.85 95.0% UCL of Median 1987.94 760 4/10/2013 10:40:48 AM 1295 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-09 Reach=R13, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 59 137 13 34 Multiplicity Factor = 0 Mean of W 114 57 Std Dev of W 10.67708 10.67708 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031997 Yes 0.984001 No 0.015999 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.1541 0.031229 Yes -2.1541 0.984386 No -2.1541 0.015614 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.1073 0.035090 Yes -2.2010 0.986131 No -2.1073 0.017545 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.666667 0.083333 0.666667 Reject H0 if Greater Than 0.6202 0.6202 0.6202 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0463 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\bass.NCSS Largemouth_Bass_PFOS_ppb 4/10/2013 10:40:48 AM 1296 5/1/2013 10:38:54 AM 1 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R02 5 43.2 6.932893 3.100484 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R02) = 2.7764 95.0% LCL of Mean 21.0515 34.59168 95.0% UCL of Mean 40.0685 51.80832 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -12.64 7.304382 Unequal 7.92 -12.64 10.32996 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3100 Standard Error 4.619697 4.619697 95.0% LCL Difference -23.29304 -23.31129 95.0% UCL Difference -1.98696 -1.96871 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.7361 0.025603 Yes Difference < 0 -2.7361 0.012801 Yes Difference > 0 -2.7361 0.987199 No Difference: (Reach=R01)-(Reach=R02) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.670064 0.802338 0.000015 Power (Alpha=.010) 0.348927 0.480309 0.000001 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.7361 0.025838 Yes Difference < 0 -2.7361 0.012919 Yes Difference > 0 -2.7361 0.987081 No Difference: (Reach=R01)-(Reach=R02) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.668863 0.801659 0.000015 Power (Alpha=.010) 0.347073 0.478578 0.000001 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.2201 0.0177 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.851792 0.897476 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R02 Count 5 5 Median 32.7 44.1 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:55 AM 2 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R02 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 3 18 22 37 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.027778 Yes 0.972222 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.9845 0.047202 Yes -1.9845 0.023601 Yes -1.9845 0.976399 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.8800 0.060103 No -1.8800 0.030051 Yes -2.0889 0.981643 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:55 AM 3 Largemouth_Bass_PFOS_ppbvs Groups 50 20 15-I---------------- 1--------------------------------- 1---- Reach=R01 Reach=R02 Groups 5/1/2013 10:38:55 AM 4 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R03 5 35.16 21.54932 9.637147 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R03) = 2.7764 95.0% LCL of Mean 21.0515 8.40299 95.0% UCL of Mean 40.0685 61.91701 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -4.6 16.17121 Unequal 4.99 -4.6 22.86954 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5714 Standard Error 10.22757 10.22757 95.0% LCL Difference -28.18482 -30.89965 95.0% UCL Difference 18.98482 21.69965 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.4498 0.664817 No Difference < 0 -0.4498 0.332408 No Difference > 0 -0.4498 0.667592 No Difference: (Reach=R01)-(Reach=R03) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.068376 0.108983 0.019754 Power (Alpha=.010) 0.015227 0.025858 0.003366 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.4498 0.671728 No Difference < 0 -0.4498 0.335864 No Difference > 0 -0.4498 0.664136 No Difference: (Reach=R01)-(Reach=R03) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.065873 0.105164 0.020690 Power (Alpha=.010) 0.014133 0.023870 0.003695 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 7.9186 0.4627 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.069793 0.515553 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R03 Count 5 5 Median 32.7 25.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:55 AM 5 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R03 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 15 30 10 25 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.690476 No 0.654762 No 0.345238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.5222 0.601508 No 0.5222 0.699246 No 0.5222 0.300754 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.4178 0.676103 No 0.6267 0.734565 No 0.4178 0.338052 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:55 AM 6 75 -, 65 a.I 55 Largemouth_Bass_PFOS_ppbvs Groups 15-I---------------- 1--------------------------------- 1---- Reach=R01 Reach=R03 Groups 5/1/2013 10:38:55 AM 7 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R04 5 207.8 133.6495 59.76989 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R04) = 2.7764 95.0% LCL of Mean 21.0515 41.85218 95.0% UCL of Mean 40.0685 373.7478 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -177.24 94.6595 Unequal 4.03 -177.24 133.8688 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7693 Standard Error 59.86793 59.86793 95.0% LCL Difference -315.2957 -343.0333 95.0% UCL Difference -39.18431 -11.44674 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.9605 0.018132 Yes Difference < 0 -2.9605 0.009066 Yes Difference > 0 -2.9605 0.990934 No Difference: (Reach=R01)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.737011 0.853793 0.000006 Power (Alpha=.010) 0.415301 0.553002 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.9605 0.041195 Yes Difference < 0 -2.9605 0.020598 Yes Difference > 0 -2.9605 0.979402 No Difference: (Reach=R01)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.610165 0.783050 0.000011 Power (Alpha=.010) 0.240274 0.378657 0.000002 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 304.5922 2.6525 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000064 0.142037 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R04 Count 5 5 Median 32.7 159 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:55 AM 8 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:55 AM 9 Largemouth_Bass_PFOS_ppbvs Groups 450 n 0J---------------- 1-------------------------------- 1---- Reach=R01 Reach=R04 Groups 5/1/2013 10:38:56 AM 10 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R05 5 404.88 192.5162 86.09584 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R05) = 2.7764 95.0% LCL of Mean 21.0515 165.8396 95.0% UCL of Mean 40.0685 643.9204 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -374.32 136.2371 Unequal 4.01 -374.32 192.6684 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7730 Standard Error 86.16393 86.16393 95.0% LCL Difference -573.0144 -613.2521 95.0% UCL Difference -175.6256 -135.3879 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.3443 0.002464 Yes Difference < 0 -4.3443 0.001232 Yes Difference > 0 -4.3443 0.998768 No Difference: (Reach=R01)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.965790 0.989217 0.000000 Power (Alpha=.010) 0.801034 0.893757 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.3443 0.012127 Yes Difference < 0 -4.3443 0.006064 Yes Difference > 0 -4.3443 0.993936 No Difference: (Reach=R01)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.894273 0.968663 0.000000 Power (Alpha=.010) 0.521952 0.703224 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 632.0016 4.5574 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000015 0.065301 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R05 Count 5 5 Median 32.7 435 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:56 AM 11 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R05 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:56 AM 12 5/1/2013 10:38:56 AM 13 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R06 5 65.46 32.25117 14.42316 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean 21.0515 25.41488 95.0% UCL of Mean 40.0685 105.5051 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -34.9 23.43908 Unequal 4.45 -34.9 33.14787 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6692 Standard Error 14.82418 14.82418 95.0% LCL Difference -69.08461 -74.46909 95.0% UCL Difference -0.7153884 4.669096 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.3543 0.046372 Yes Difference < 0 -2.3543 0.023186 Yes Difference > 0 -2.3543 0.976814 No Difference: (Reach=R01)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.543381 0.692955 0.000066 Power (Alpha=.010) 0.246203 0.358858 0.000006 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.3543 0.071592 No Difference < 0 -2.3543 0.035796 Yes Difference > 0 -2.3543 0.964204 No Difference: (Reach=R01)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.456015 0.632222 0.000101 Power (Alpha=.010) 0.159609 0.261748 0.000014 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 17.7368 1.3255 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.016483 0.282852 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R06 Count 5 5 Median 32.7 50.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:56 AM 14 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:56 AM 15 12 n Largemouth_Bass_PFOS_ppb vs Groups --------i-- 5/1/2013 10:38:56 AM 16 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R07 5 286.2 87.50543 39.13362 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 21.0515 177.5477 95.0% UCL of Mean 40.0685 394.8523 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -255.64 62.11217 Unequal 4.06 -255.64 87.83987 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7600 Standard Error 39.28318 39.28318 95.0% LCL Difference -346.2272 -364.0618 95.0% UCL Difference -165.0528 -147.2182 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -6.5076 0.000187 Yes Difference < 0 -6.5076 0.000093 Yes Difference > 0 -6.5076 0.999907 No Difference: (Reach=R01)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999870 0.999988 0.000000 Power (Alpha=.010) 0.992951 0.998406 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -6.5076 0.002729 Yes Difference < 0 -6.5076 0.001365 Yes Difference > 0 -6.5076 0.998635 No Difference: (Reach=R01)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996803 0.999784 0.000000 Power (Alpha=.010) 0.882471 0.964181 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 130.5731 4.6463 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000345 0.063228 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R07 Count 5 5 Median 32.7 262 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:56 AM 17 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:56 AM 18 5/1/2013 10:38:56 AM 19 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R08 5 102.24 51.72672 23.13289 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 21.0515 38.0128 95.0% UCL of Mean 40.0685 166.4672 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -71.68 36.97496 Unequal 4.18 -71.68 52.2905 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7311 Standard Error 23.38502 23.38502 95.0% LCL Difference -125.606 -135.5464 95.0% UCL Difference -17.75405 -7.813577 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0652 0.015460 Yes Difference < 0 -3.0652 0.007730 Yes Difference > 0 -3.0652 0.992270 No Difference: (Reach=R01)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.765640 0.874389 0.000004 Power (Alpha=.010) 0.447149 0.586382 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0652 0.035403 Yes Difference < 0 -3.0652 0.017702 Yes Difference > 0 -3.0652 0.982298 No Difference: (Reach=R01)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.648139 0.812090 0.000007 Power (Alpha=.010) 0.269825 0.415674 0.000001 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 45.6261 3.2933 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.002720 0.107111 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R08 Count 5 5 Median 32.7 80.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:56 AM 20 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:57 AM 21 5/1/2013 10:38:57 AM 22 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R09 5 231.8 57.10692 25.53899 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 21.0515 160.8924 95.0% UCL of Mean 40.0685 302.7076 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -201.24 40.74213 Unequal 4.14 -201.24 57.61808 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7389 Standard Error 25.76759 25.76759 95.0% LCL Difference -260.6602 -271.8137 95.0% UCL Difference -141.8198 -130.6663 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.8098 0.000052 Yes Difference < 0 -7.8098 0.000026 Yes Difference > 0 -7.8098 0.999974 No Difference: (Reach=R01)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999999 1.000000 0.000000 Power (Alpha=.010) 0.999665 0.999962 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.8098 0.001253 Yes Difference < 0 -7.8098 0.000627 Yes Difference > 0 -7.8098 0.999373 No Difference: (Reach=R01)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999856 0.999997 0.000000 Power (Alpha=.010) 0.969896 0.994996 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 55.6111 4.1491 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001850 0.076034 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R09 Count 5 5 Median 32.7 259 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:57 AM 23 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:57 AM 24 5/1/2013 10:38:57 AM 25 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R10 5 133.62 61.49823 27.50284 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R10) = 2.7764 95.0% LCL of Mean 21.0515 57.25986 95.0% UCL of Mean 40.0685 209.9801 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -103.06 43.82166 Unequal 4.12 -103.06 61.97318 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7438 Standard Error 27.71525 27.71525 95.0% LCL Difference -166.9715 -179.1058 95.0% UCL Difference -39.14852 -27.0142 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.7185 0.005884 Yes Difference < 0 -3.7185 0.002942 Yes Difference > 0 -3.7185 0.997058 No Difference: (Reach=R01)-(Reach=R10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.900915 0.958726 0.000000 Power (Alpha=.010) 0.644128 0.772117 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.7185 0.019416 Yes Difference < 0 -3.7185 0.009708 Yes Difference > 0 -3.7185 0.990292 No Difference: (Reach=R01)-(Reach=R10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.799368 0.918625 0.000000 Power (Alpha=.010) 0.400287 0.573936 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 64.4925 3.6337 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001385 0.093069 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R10 Count 5 5 Median 32.7 104 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:57 AM 26 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:57 AM 27 5/1/2013 10:38:57 AM 28 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 21.0515 97.42249 95.0% UCL of Mean 40.0685 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -131.64 37.285 Unequal 4.17 -131.64 52.72896 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7318 Standard Error 23.58111 23.58111 95.0% LCL Difference -186.0181 -196.0591 95.0% UCL Difference -77.26187 -67.22094 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.5824 0.000521 Yes Difference < 0 -5.5824 0.000260 Yes Difference > 0 -5.5824 0.999740 No Difference: (Reach=R01)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.997970 0.999668 0.000000 Power (Alpha=.010) 0.961860 0.987001 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.5824 0.004452 Yes Difference < 0 -5.5824 0.002226 Yes Difference > 0 -5.5824 0.997774 No Difference: (Reach=R01)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.984167 0.997834 0.000000 Power (Alpha=.010) 0.779400 0.906952 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 46.4113 6.0276 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.002632 0.039620 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R01 Reach=R11 Count 5 5 Median 32.7 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:57 AM 29 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:57 AM 30 5/1/2013 10:38:58 AM 31 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 21.0515 49.75645 95.0% UCL of Mean 40.0685 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -39.56 12.79863 Unequal 5.67 -39.56 18.1 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4824 Standard Error 8.094566 8.094566 95.0% LCL Difference -58.2261 -59.65402 95.0% UCL Difference -20.8939 -19.46598 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.8872 0.001213 Yes Difference < 0 -4.8872 0.000607 Yes Difference > 0 -4.8872 0.999393 No Difference: (Reach=R01)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.988830 0.997314 0.000000 Power (Alpha=.010) 0.895113 0.953337 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.8872 0.003220 Yes Difference < 0 -4.8872 0.001610 Yes Difference > 0 -4.8872 0.998390 No Difference: (Reach=R01)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.977547 0.995011 0.000000 Power (Alpha=.010) 0.801339 0.906403 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.5865 0.2704 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.169309 0.617147 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R12 Count 5 5 Median 32.7 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:58 AM 32 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:58 AM 33 100 n 85 Largemouth_Bass_PFOS_ppb vs Groups 10 0------------------- 1--------------------------------------- T----- Reach=R01 Reach=R12 Groups 5/1/2013 10:38:58 AM 34 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R13) = 2.7764 95.0% LCL of Mean 21.0515 60.96663 95.0% UCL of Mean 40.0685 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -46.46 10.62544 Unequal 6.50 -46.46 15.02664 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4021 Standard Error 6.720119 6.720119 95.0% LCL Difference -61.95662 -62.60227 95.0% UCL Difference -30.96338 -30.31773 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -6.9136 0.000123 Yes Difference < 0 -6.9136 0.000061 Yes Difference > 0 -6.9136 0.999939 No Difference: (Reach=R01)-(Reach=R13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999967 0.999998 0.000000 Power (Alpha=.010) 0.997033 0.999451 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -6.9136 0.000319 Yes Difference < 0 -6.9136 0.000160 Yes Difference > 0 -6.9136 0.999840 No Difference: (Reach=R01)-(Reach=R13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999912 0.999995 0.000000 Power (Alpha=.010) 0.991678 0.998417 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.8504 0.2971 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.334632 0.600591 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R13 Count 5 5 Median 32.7 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:58 AM 35 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:58 AM 36 5/1/2013 10:38:58 AM 37 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 21.0515 50.37198 95.0% UCL of Mean 40.0685 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -42.76 14.14594 Unequal 5.33 -42.76 20.00537 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5229 Standard Error 8.946675 8.946675 95.0% LCL Difference -63.39107 -65.3315 95.0% UCL Difference -22.12893 -20.18849 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.7794 0.001392 Yes Difference < 0 -4.7794 0.000696 Yes Difference > 0 -4.7794 0.999304 No Difference: (Reach=R01)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.985843 0.996401 0.000000 Power (Alpha=.010) 0.879661 0.944386 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.7794 0.004196 Yes Difference < 0 -4.7794 0.002098 Yes Difference > 0 -4.7794 0.997902 No Difference: (Reach=R01)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.968853 0.992704 0.000000 Power (Alpha=.010) 0.757116 0.879331 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.8246 4.1400 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.116240 0.076298 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R14 Count 5 5 Median 32.7 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:58 AM 38 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:58 AM 39 100 n Largemouth_Bass_PFOS_ppb vs Groups 100------------------- 1--------------------------------------- t----- Reach=R01 Reach=R14 Groups 5/1/2013 10:38:58 AM 40 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 30.56 7.657872 3.424704 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R15) = 2.7764 95.0% LCL of Mean 21.0515 66.08784 95.0% UCL of Mean 40.0685 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -54.38 12.02427 Unequal 5.91 -54.38 17.00488 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4558 Standard Error 7.604814 7.604814 95.0% LCL Difference -71.91673 -73.0561 95.0% UCL Difference -36.84327 -35.7039 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.1507 0.000097 Yes Difference < 0 -7.1507 0.000049 Yes Difference > 0 -7.1507 0.999951 No Difference: (Reach=R01)-(Reach=R15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999986 0.999999 0.000000 Power (Alpha=.010) 0.998272 0.999717 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.1507 0.000403 Yes Difference < 0 -7.1507 0.000202 Yes Difference > 0 -7.1507 0.999798 No Difference: (Reach=R01)-(Reach=R15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999930 0.999997 0.000000 Power (Alpha=.010) 0.990928 0.998424 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.9310 1.8692 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.213405 0.208745 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R15 Count 5 5 Median 32.7 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:58 AM 41 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:59 AM 42 110 n Largemouth_Bass_PFOS_ppb vs Groups 1 0 J -------------------------------------1-------------------------------------------------------------------------- T---------- Reach=R01 Reach=R15 Groups 5/1/2013 10:38:59 AM 43 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R03 5 35.16 21.54932 9.637147 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R03) = 2.7764 95.0% LCL of Mean 34.59168 8.40299 95.0% UCL of Mean 51.80832 61.91701 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 8.04 16.00684 Unequal 4.82 8.04 22.63709 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5999 Standard Error 10.12362 10.12362 95.0% LCL Difference -15.3051 -18.27989 95.0% UCL Difference 31.3851 34.35989 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7942 0.450003 No Difference < 0 0.7942 0.774998 No Difference > 0 0.7942 0.225002 No Difference: (Reach=R02)-(Reach=R03) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.108225 0.008736 0.179699 Power (Alpha=.010) 0.027505 0.001327 0.048902 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7942 0.464381 No Difference < 0 0.7942 0.767810 No Difference > 0 0.7942 0.232190 No Difference: (Reach=R02)-(Reach=R03) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.099332 0.009619 0.169111 Power (Alpha=.010) 0.023247 0.001600 0.042388 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 9.6614 0.5356 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.049486 0.485127 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R03 Count 5 5 Median 44.1 25.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:59 AM 44 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R03 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 35 5 20 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.924603 No 0.075397 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.5667 0.117185 No 1.5667 0.941407 No 1.5667 0.058593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.4623 0.143672 No 1.6711 0.952654 No 1.4623 0.071836 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.200000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:59 AM 45 5/1/2013 10:38:59 AM 46 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R04 5 207.8 133.6495 59.76989 Note: T-alpha (R e ac h =R02) = 2.7764, T-alpha (Reach=R04) = 2.7764 95.0% LCL of Mean 34.59168 41.85218 95.0% UCL of Mean 51.80832 373.7478 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -164.6 94.63156 Unequal 4.02 -164.6 133.8292 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7706 Standard Error 59.85025 59.85025 95.0% LCL Difference -302.6149 -330.4207 95.0% UCL Difference -26.58507 1.220734 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.7502 0.025051 Yes Difference < 0 -2.7502 0.012526 Yes Difference > 0 -2.7502 0.987474 No Difference: (Reach=R02)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.674467 0.805861 0.000014 Power (Alpha=.010) 0.352992 0.484886 0.000001 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.7502 0.051061 No Difference < 0 -2.7502 0.025531 Yes Difference > 0 -2.7502 0.974469 No Difference: (Reach=R02)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.550719 0.730676 0.000026 Power (Alpha=.010) 0.203913 0.328822 0.000004 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 371.6259 2.6816 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000043 0.140151 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R04 Count 5 5 Median 44.1 159 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:59 AM 47 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:59 AM 48 450 n Largemouth_Bass_PFOS_ppb vs Groups 0 J------------------- 1--------------------------------------- 1----- Reach=R02 Reach=R04 Groups 5/1/2013 10:38:59 AM 49 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R05 5 404.88 192.5162 86.09584 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R05) = 2.7764 95.0% LCL of Mean 34.59168 165.8396 95.0% UCL of Mean 51.80832 643.9204 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -361.68 136.2177 Unequal 4.01 -361.68 192.641 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7736 Standard Error 86.15166 86.15166 95.0% LCL Difference -560.3461 -600.6315 95.0% UCL Difference -163.0139 -122.7285 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.1982 0.003004 Yes Difference < 0 -4.1982 0.001502 Yes Difference > 0 -4.1982 0.998498 No Difference: (Reach=R02)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.955196 0.984880 0.000000 Power (Alpha=.010) 0.768734 0.870799 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.1982 0.013641 Yes Difference < 0 -4.1982 0.006821 Yes Difference > 0 -4.1982 0.993179 No Difference: (Reach=R02)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.874536 0.959789 0.000000 Power (Alpha=.010) 0.490495 0.672180 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 771.0906 4.5894 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000010 0.064545 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R05 Count 5 5 Median 44.1 435 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:59 AM 50 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R05 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:38:59 AM 51 5/1/2013 10:38:59 AM 52 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R06 5 65.46 32.25117 14.42316 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean 34.59168 25.41488 95.0% UCL of Mean 51.80832 105.5051 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -22.26 23.32598 Unequal 4.37 -22.26 32.98792 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6864 Standard Error 14.75265 14.75265 95.0% LCL Difference -56.27967 -61.89135 95.0% UCL Difference 11.75967 17.37135 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.5089 0.169765 No Difference < 0 -1.5089 0.084882 No Difference > 0 -1.5089 0.915118 No Difference: (Reach=R02)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.265274 0.395888 0.001191 Power (Alpha=.010) 0.087809 0.144815 0.000146 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.5089 0.199915 No Difference < 0 -1.5089 0.099958 No Difference > 0 -1.5089 0.900042 No Difference: (Reach=R02)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.222855 0.356462 0.001540 Power (Alpha=.010) 0.061178 0.109384 0.000232 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 21.6402 1.4151 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.011361 0.268321 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R06 Count 5 5 Median 44.1 50.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:38:59 AM 53 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R06 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 5.5 20.5 19.5 34.5 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.4667 0.142457 No -1.4667 0.071228 No -1.4667 0.928772 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.3619 0.173217 No -1.3619 0.086609 No -1.5715 0.941963 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:00 AM 54 12 n 105 Largemouth_Bass_PFOS_ppb vs Groups -----i-- 30 Reach=R02 Reach=R06 Groups 5/1/2013 10:39:00 AM 55 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R07 5 286.2 87.50543 39.13362 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 34.59168 177.5477 95.0% UCL of Mean 51.80832 394.8523 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -243 62.06958 Unequal 4.05 -243 87.77964 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7629 Standard Error 39.25625 39.25625 95.0% LCL Difference -333.5251 -351.4619 95.0% UCL Difference -152.4749 -134.538 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -6.1901 0.000262 Yes Difference < 0 -6.1901 0.000131 Yes Difference > 0 -6.1901 0.999869 No Difference: (Reach=R02)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999645 0.999960 0.000000 Power (Alpha=.010) 0.986846 0.996551 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -6.1901 0.003322 Yes Difference < 0 -6.1901 0.001661 Yes Difference > 0 -6.1901 0.998339 No Difference: (Reach=R02)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.993984 0.999475 0.000000 Power (Alpha=.010) 0.846856 0.947046 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 159.3093 4.7251 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000233 0.061461 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R07 Count 5 5 Median 44.1 262 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:00 AM 56 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:00 AM 57 5/1/2013 10:39:00 AM 58 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R08 5 102.24 51.72672 23.13289 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 34.59168 38.0128 95.0% UCL of Mean 51.80832 166.4672 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -59.04 36.90337 Unequal 4.14 -59.04 52.18925 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7389 Standard Error 23.33974 23.33974 95.0% LCL Difference -112.8615 -122.965 95.0% UCL Difference -5.218456 4.884997 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.5296 0.035278 Yes Difference < 0 -2.5296 0.017639 Yes Difference > 0 -2.5296 0.982361 No Difference: (Reach=R02)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.603007 0.746366 0.000033 Power (Alpha=.010) 0.291411 0.413724 0.000003 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.5296 0.062565 No Difference < 0 -2.5296 0.031282 Yes Difference > 0 -2.5296 0.968718 No Difference: (Reach=R02)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.493650 0.674358 0.000057 Power (Alpha=.010) 0.174742 0.286138 0.000008 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 55.6674 3.3981 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001846 0.102509 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R08 Count 5 5 Median 44.1 80.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:00 AM 59 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 2 17 23 38 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.015873 Yes 0.984127 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.1934 0.028280 Yes -2.1934 0.014140 Yes -2.1934 0.985860 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.0889 0.036714 Yes -2.0889 0.018357 Yes -2.2978 0.989214 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:00 AM 60 5/1/2013 10:39:00 AM 61 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R09 5 231.8 57.10692 25.53899 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 34.59168 160.8924 95.0% UCL of Mean 51.80832 302.7076 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -188.6 40.67717 Unequal 4.12 -188.6 57.52621 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7454 Standard Error 25.7265 25.7265 95.0% LCL Difference -247.9254 -259.229 95.0% UCL Difference -129.2746 -117.971 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.3310 0.000081 Yes Difference < 0 -7.3310 0.000041 Yes Difference > 0 -7.3310 0.999959 No Difference: (Reach=R02)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999993 1.000000 0.000000 Power (Alpha=.010) 0.998874 0.999832 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.3310 0.001645 Yes Difference < 0 -7.3310 0.000822 Yes Difference > 0 -7.3310 0.999178 No Difference: (Reach=R02)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999513 0.999984 0.000000 Power (Alpha=.010) 0.948203 0.989089 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 67.8498 4.2595 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001253 0.072921 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R09 Count 5 5 Median 44.1 259 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:00 AM 62 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:00 AM 63 5/1/2013 10:39:00 AM 64 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R10 5 133.62 61.49823 27.50284 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 34.59168 57.25986 95.0% UCL of Mean 51.80832 209.9801 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -90.42 43.76127 Unequal 4.10 -90.42 61.88778 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7495 Standard Error 27.67706 27.67706 95.0% LCL Difference -154.2434 -166.5185 95.0% UCL Difference -26.5966 -14.32146 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.2670 0.011408 Yes Difference < 0 -3.2670 0.005704 Yes Difference > 0 -3.2670 0.994296 No Difference: (Reach=R 02)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.815551 0.908145 0.000001 Power (Alpha=.010) 0.509139 0.648656 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.2670 0.029763 Yes Difference < 0 -3.2670 0.014882 Yes Difference > 0 -3.2670 0.985118 No Difference: (Reach=R 02)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.696009 0.849886 0.000003 Power (Alpha=.010) 0.303864 0.460033 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 78.6858 3.7223 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000937 0.089813 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R10 Count 5 5 Median 44.1 104 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:01 AM 65 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:01 AM 66 5/1/2013 10:39:01 AM 67 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 34.59168 97.42249 95.0% UCL of Mean 51.80832 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -119 37.21401 Unequal 4.14 -119 52.62856 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7395 Standard Error 23.53621 23.53621 95.0% LCL Difference -173.2746 -183.4774 95.0% UCL Difference -64.72541 -54.52263 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.0560 0.000982 Yes Difference < 0 -5.0560 0.000491 Yes Difference > 0 -5.0560 0.999509 No Difference: (Reach=R02)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.992406 0.998331 0.000000 Power (Alpha=.010) 0.916298 0.964978 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.0560 0.006565 Yes Difference < 0 -5.0560 0.003282 Yes Difference > 0 -5.0560 0.996718 No Difference: (Reach=R02)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.962257 0.992660 0.000000 Power (Alpha=.010) 0.684499 0.841392 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 56.6254 6.2250 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001786 0.037231 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R02 Reach=R11 Count 5 5 Median 44.1 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:01 AM 68 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:01 AM 69 5/1/2013 10:39:01 AM 70 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 34.59168 49.75645 95.0% UCL of Mean 51.80832 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -26.92 12.59031 Unequal 5.39 -26.92 17.80539 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5162 Standard Error 7.962813 7.962813 95.0% LCL Difference -45.28228 -46.95625 95.0% UCL Difference -8.557719 -6.883755 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3807 0.009631 Yes Difference < 0 -3.3807 0.004816 Yes Difference > 0 -3.3807 0.995184 No Difference: (Reach=R02)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.840493 0.923922 0.000001 Power (Alpha=.010) 0.543987 0.682121 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3807 0.017528 Yes Difference < 0 -3.3807 0.008764 Yes Difference > 0 -3.3807 0.991236 No Difference: (Reach=R02)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.784620 0.899044 0.000001 Power (Alpha=.010) 0.423618 0.581669 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.5959 0.3526 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.123973 0.569070 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R12 Count 5 5 Median 44.1 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:01 AM 71 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:01 AM 72 100n 90 Largemouth_Bass_PFOS_ppb vs Groups 303----------------1-------------------------------- t---- Reach=R02 Reach=R12 Groups 5/1/2013 10:39:01 AM 73 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 34.59168 60.96663 95.0% UCL of Mean 51.80832 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -33.82 10.37357 Unequal 6.12 -33.82 14.67045 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4349 Standard Error 6.560823 6.560823 95.0% LCL Difference -48.94928 -49.79487 95.0% UCL Difference -18.69071 -17.84513 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.1548 0.000869 Yes Difference < 0 -5.1548 0.000435 Yes Difference > 0 -5.1548 0.999565 No Difference: (Reach=R 02)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.993991 0.998748 0.000000 Power (Alpha=.010) 0.927086 0.970597 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.1548 0.001979 Yes Difference < 0 -5.1548 0.000990 Yes Difference > 0 -5.1548 0.999010 No Difference: (Reach=R 02)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.988990 0.997875 0.000000 Power (Alpha=.010) 0.870164 0.945493 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.4777 0.4087 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.254697 0.540485 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R13 Count 5 5 Median 44.1 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:01 AM 74 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:01 AM 75 100n 90 Largemouth_Bass_PFOS_ppb vs Groups 303----------------1-------------------------------- t---- Reach=R02 Reach=R13 Groups 5/1/2013 10:39:02 AM 76 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 34.59168 50.37198 95.0% UCL of Mean 51.80832 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -30.12 13.95774 Unequal 5.10 -30.12 19.73923 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5549 Standard Error 8.82765 8.82765 95.0% LCL Difference -50.4766 -52.67398 95.0% UCL Difference -9.763403 -7.566017 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.4120 0.009196 Yes Difference < 0 -3.4120 0.004598 Yes Difference > 0 -3.4120 0.995402 No Difference: (Reach=R02)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.846943 0.927876 0.000001 Power (Alpha=.010) 0.553514 0.691077 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.4120 0.018398 Yes Difference < 0 -3.4120 0.009199 Yes Difference > 0 -3.4120 0.990801 No Difference: (Reach=R02)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.781535 0.898986 0.000001 Power (Alpha=.010) 0.412854 0.573317 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 7.1065 4.9875 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.083795 0.056010 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R14 Count 5 5 Median 44.1 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:02 AM 77 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:02 AM 78 Largemouth_Bass_PFOS_ppb vs Groups 95 30 0--------------------- t------------------------------------------ t------ Reach=R02 Reach=R14 Groups 5/1/2013 10:39:02 AM 79 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 43.2 6.932893 3.100484 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 34.59168 66.08784 95.0% UCL of Mean 51.80832 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -41.74 11.80229 Unequal 5.60 -41.74 16.69096 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4901 Standard Error 7.464422 7.464422 95.0% LCL Difference -58.95299 -60.32701 95.0% UCL Difference -24.52701 -23.15298 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.5919 0.000515 Yes Difference < 0 -5.5919 0.000258 Yes Difference > 0 -5.5919 0.999742 No Difference: (Reach=R 02)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.998021 0.999678 0.000000 Power (Alpha=.010) 0.962436 0.987246 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.5919 0.001751 Yes Difference < 0 -5.5919 0.000875 Yes Difference > 0 -5.5919 0.999125 No Difference: (Reach=R 02)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.994428 0.999204 0.000000 Power (Alpha=.010) 0.899678 0.963472 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.7961 2.3667 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.158058 0.162508 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R15 Count 5 5 Median 44.1 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:02 AM 80 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:02 AM 81 5/1/2013 10:39:02 AM 82 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R04 5 207.8 133.6495 59.76989 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R04) = 2.7764 95.0% LCL of Mean 8.40299 41.85218 95.0% UCL of Mean 61.91701 373.7478 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -172.64 95.72506 Unequal 4.21 -172.64 135.3757 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7232 Standard Error 60.54184 60.54184 95.0% LCL Difference -312.2497 -337.5068 95.0% UCL Difference -33.03026 -7.773191 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.8516 0.021427 Yes Difference < 0 -2.8516 0.010713 Yes Difference > 0 -2.8516 0.989287 No Difference: (Reach=R03)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.705401 0.830062 0.000009 Power (Alpha=.010) 0.382682 0.517815 0.000001 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.8516 0.043655 Yes Difference < 0 -2.8516 0.021828 Yes Difference > 0 -2.8516 0.978172 No Difference: (Reach=R03)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.590794 0.763743 0.000017 Power (Alpha=.010) 0.231971 0.365252 0.000002 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 38.4652 2.1779 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.003787 0.178242 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R04 Count 5 5 Median 25.7 159 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:02 AM 83 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:02 AM 84 0 J-------------------- 1------------------------------------------1------ Reach=R03 Reach=RQ4 Groups 5/1/2013 10:39:02 AM 85 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R05 5 404.88 192.5162 86.09584 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R05) = 2.7764 95.0% LCL of Mean 8.40299 165.8396 95.0% UCL of Mean 61.91701 643.9204 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -369.72 136.9796 Unequal 4.10 -369.72 193.7185 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7499 Standard Error 86.63353 86.63353 95.0% LCL Difference -569.4973 -607.9522 95.0% UCL Difference -169.9427 -131.4878 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.2676 0.002733 Yes Difference < 0 -4.2676 0.001366 Yes Difference > 0 -4.2676 0.998634 No Difference: (Reach=R03)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.960524 0.987100 0.000000 Power (Alpha=.010) 0.784450 0.882115 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.2676 0.012301 Yes Difference < 0 -4.2676 0.006151 Yes Difference > 0 -4.2676 0.993849 No Difference: (Reach=R03)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.888738 0.965679 0.000000 Power (Alpha=.010) 0.517124 0.696812 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 79.8119 4.0185 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000911 0.079940 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R05 Count 5 5 Median 25.7 435 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:02 AM 86 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R05 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:03 AM 87 Largemouth_Bass_PFOS_ppb vs Groups Reach=R03 Groups Reach=RQ5 5/1/2013 10:39:03 AM 88 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R06 5 65.46 32.25117 14.42316 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean 8.40299 25.41488 95.0% UCL of Mean 61.91701 105.5051 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -30.3 27.42728 Unequal 6.98 -30.3 38.78802 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3661 Standard Error 17.34653 17.34653 95.0% LCL Difference -70.30118 -71.34422 95.0% UCL Difference 9.701177 10.74421 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.7467 0.118819 No Difference < 0 -1.7467 0.059410 No Difference > 0 -1.7467 0.940590 No Difference: (Reach=R03)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.337279 0.481076 0.000559 Power (Alpha=.010) 0.121913 0.194399 0.000064 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.7467 0.124316 No Difference < 0 -1.7467 0.062158 No Difference > 0 -1.7467 0.937842 No Difference: (Reach=R03)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.326629 0.472364 0.000594 Power (Alpha=.010) 0.113294 0.184075 0.000072 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.2399 0.3007 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.453985 0.598410 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R06 Count 5 5 Median 25.7 50.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:03 AM 89 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 4 19 21 36 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.047619 Yes 0.952381 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.7756 0.075800 No -1.7756 0.037900 Yes -1.7756 0.962100 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.6711 0.094693 No -1.6711 0.047346 Yes -1.8800 0.969949 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:03 AM 90 12 n 100 Largemouth_Bass_PFOS_ppb vs Groups -----i-- 20 Reach=R03 Reach=R06 Groups 5/1/2013 10:39:03 AM 91 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R07 5 286.2 87.50543 39.13362 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 8.40299 177.5477 95.0% UCL of Mean 61.91701 394.8523 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -251.04 63.7243 Unequal 4.48 -251.04 90.11977 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6623 Standard Error 40.30279 40.30279 95.0% LCL Difference -343.9784 -358.3373 95.0% UCL Difference -158.1016 -143.7427 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -6.2288 0.000251 Yes Difference < 0 -6.2288 0.000126 Yes Difference > 0 -6.2288 0.999874 No Difference: (Reach=R03)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999685 0.999965 0.000000 Power (Alpha=.010) 0.987780 0.996852 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -6.2288 0.002296 Yes Difference < 0 -6.2288 0.001148 Yes Difference > 0 -6.2288 0.998852 No Difference: (Reach=R03)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996584 0.999708 0.000000 Power (Alpha=.010) 0.894808 0.966854 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 16.4893 3.3208 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.018868 0.105878 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R07 Count 5 5 Median 25.7 262 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:03 AM 92 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:03 AM 93 5/1/2013 10:39:03 AM 94 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R08 5 102.24 51.72672 23.13289 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 8.40299 38.0128 95.0% UCL of Mean 61.91701 166.4672 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -67.08 39.62339 Unequal 5.35 -67.08 56.03593 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5211 Standard Error 25.06003 25.06003 95.0% LCL Difference -124.8685 -130.2588 95.0% UCL Difference -9.291463 -3.901229 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.6768 0.028066 Yes Difference < 0 -2.6768 0.014033 Yes Difference > 0 -2.6768 0.985967 No Difference: (Reach=R03)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.651253 0.787078 0.000019 Power (Alpha=.010) 0.331989 0.461059 0.000002 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.6768 0.041107 Yes Difference < 0 -2.6768 0.020553 Yes Difference > 0 -2.6768 0.979447 No Difference: (Reach=R03)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.590113 0.750414 0.000026 Power (Alpha=.010) 0.252147 0.380674 0.000003 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.7619 1.7076 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.118288 0.227611 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R08 Count 5 5 Median 25.7 80.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:03 AM 95 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 1 16 24 39 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.007937 Yes 0.992063 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.4023 0.016294 Yes -2.4023 0.008147 Yes -2.4023 0.991853 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.2978 0.021572 Yes -2.2978 0.010786 Yes -2.5067 0.993907 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:03 AM 96 5/1/2013 10:39:03 AM 97 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R09 5 231.8 57.10692 25.53899 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 8.40299 160.8924 95.0% UCL of Mean 61.91701 302.7076 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -196.64 43.16001 Unequal 5.12 -196.64 61.03747 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5531 Standard Error 27.29679 27.29679 95.0% LCL Difference -259.5865 -266.3307 95.0% UCL Difference -133.6935 -126.9492 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.2038 0.000092 Yes Difference < 0 -7.2038 0.000046 Yes Difference > 0 -7.2038 0.999954 No Difference: (Reach=R03)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999989 0.999999 0.000000 Power (Alpha=.010) 0.998474 0.999757 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.2038 0.000729 Yes Difference < 0 -7.2038 0.000364 Yes Difference > 0 -7.2038 0.999636 No Difference: (Reach=R03)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999852 0.999994 0.000000 Power (Alpha=.010) 0.981330 0.996578 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 7.0228 2.3980 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.085472 0.160082 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R09 Count 5 5 Median 25.7 259 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:03 AM 98 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:04 AM 99 50 0J------------------- 1--------------------------------------- 1----- Reach=R03 Reach=R09 Groups 5/1/2013 10:39:04 AM 100 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R10 5 133.62 61.49823 27.50284 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 8.40299 57.25986 95.0% UCL of Mean 61.91701 209.9801 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -98.46 46.07822 Unequal 4.97 -98.46 65.16444 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5756 Standard Error 29.14243 29.14243 95.0% LCL Difference -165.6626 -173.5198 95.0% UCL Difference -31.25744 -23.40019 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3786 0.009662 Yes Difference < 0 -3.3786 0.004831 Yes Difference > 0 -3.3786 0.995169 No Difference: (Reach=R 03)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.840046 0.923646 0.000001 Power (Alpha=.010) 0.543336 0.681505 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3786 0.019903 Yes Difference < 0 -3.3786 0.009952 Yes Difference > 0 -3.3786 0.990048 No Difference: (Reach=R 03)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.768362 0.891393 0.000002 Power (Alpha=.010) 0.395031 0.555381 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 8.1444 2.2204 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.066522 0.174526 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R10 Count 5 5 Median 25.7 104 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:04 AM 101 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 1 16 24 39 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.007937 Yes 0.992063 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.4023 0.016294 Yes -2.4023 0.008147 Yes -2.4023 0.991853 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.2978 0.021572 Yes -2.2978 0.010786 Yes -2.5067 0.993907 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:04 AM 102 5/1/2013 10:39:04 AM 103 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 8.40299 97.42249 95.0% UCL of Mean 61.91701 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -127.04 39.91286 Unequal 5.33 -127.04 56.44531 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5239 Standard Error 25.24311 25.24311 95.0% LCL Difference -185.2507 -190.7517 95.0% UCL Difference -68.82928 -63.32829 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.0327 0.001011 Yes Difference < 0 -5.0327 0.000505 Yes Difference > 0 -5.0327 0.999495 No Difference: (Reach=R03)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.991980 0.998215 0.000000 Power (Alpha=.010) 0.913576 0.963525 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.0327 0.003341 Yes Difference < 0 -5.0327 0.001671 Yes Difference > 0 -5.0327 0.998329 No Difference: (Reach=R03)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.980107 0.995990 0.000000 Power (Alpha=.010) 0.803313 0.910267 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.8610 2.9848 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.115075 0.122318 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R11 Count 5 5 Median 25.7 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:04 AM 104 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:04 AM 105 5/1/2013 10:39:04 AM 106 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 8.40299 49.75645 95.0% UCL of Mean 61.91701 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -34.96 19.14863 Unequal 7.47 -34.96 27.08025 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3348 Standard Error 12.11066 12.11066 95.0% LCL Difference -62.88722 -63.23616 95.0% UCL Difference -7.032776 -6.683838 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.8867 0.020301 Yes Difference < 0 -2.8867 0.010151 Yes Difference > 0 -2.8867 0.989849 No Difference: (Reach=R03)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.715789 0.837973 0.000008 Power (Alpha=.010) 0.393131 0.529198 0.000001 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.8867 0.021835 Yes Difference < 0 -2.8867 0.010918 Yes Difference > 0 -2.8867 0.989082 No Difference: (Reach=R03)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.707095 0.833324 0.000008 Power (Alpha=.010) 0.378451 0.515929 0.000001 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.7265 0.0684 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.609769 0.800248 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R12 Count 5 5 Median 25.7 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:04 AM 107 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 4 19 21 36 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.047619 Yes 0.952381 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.7756 0.075800 No -1.7756 0.037900 Yes -1.7756 0.962100 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.6711 0.094693 No -1.6711 0.047346 Yes -1.8800 0.969949 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:05 AM 108 5/1/2013 10:39:05 AM 109 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 8.40299 60.96663 95.0% UCL of Mean 61.91701 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -41.86 17.76978 Unequal 6.55 -41.86 25.13026 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3980 Standard Error 11.23859 11.23859 95.0% LCL Difference -67.77625 -68.81025 95.0% UCL Difference -15.94376 -14.90975 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.7247 0.005832 Yes Difference < 0 -3.7247 0.002916 Yes Difference > 0 -3.7247 0.997084 No Difference: (Reach=R 03)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.901829 0.959211 0.000000 Power (Alpha=.010) 0.645874 0.773604 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.7247 0.008358 Yes Difference < 0 -3.7247 0.004179 Yes Difference > 0 -3.7247 0.995821 No Difference: (Reach=R 03)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.881896 0.951758 0.000000 Power (Alpha=.010) 0.584701 0.729359 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.7781 0.1044 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.346177 0.754873 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R13 Count 5 5 Median 25.7 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:05 AM 110 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 3 18 22 37 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.027778 Yes 0.972222 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.9845 0.047202 Yes -1.9845 0.023601 Yes -1.9845 0.976399 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.8800 0.060103 No -1.8800 0.030051 Yes -2.0889 0.981643 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:05 AM 111 5/1/2013 10:39:05 AM 112 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 8.40299 50.37198 95.0% UCL of Mean 61.91701 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -38.16 20.07417 Unequal 7.82 -38.16 28.38917 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3154 Standard Error 12.69602 12.69602 95.0% LCL Difference -67.43708 -67.55582 95.0% UCL Difference -8.882918 -8.764184 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0057 0.016925 Yes Difference < 0 -3.0057 0.008462 Yes Difference > 0 -3.0057 0.991538 No Difference: (Reach=R03)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.749576 0.862939 0.000005 Power (Alpha=.010) 0.428988 0.567464 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0057 0.017378 Yes Difference < 0 -3.0057 0.008689 Yes Difference > 0 -3.0057 0.991311 No Difference: (Reach=R03)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.746789 0.861524 0.000005 Power (Alpha=.010) 0.423799 0.562952 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.3595 0.0912 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.773215 0.770350 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R14 Count 5 5 Median 25.7 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:05 AM 113 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 2 17 23 38 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.015873 Yes 0.984127 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.1934 0.028280 Yes -2.1934 0.014140 Yes -2.1934 0.985860 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.0889 0.036714 Yes -2.0889 0.018357 Yes -2.2978 0.989214 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:05 AM 114 5/1/2013 10:39:05 AM 115 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 35.16 21.54932 9.637147 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 8.40299 66.08784 95.0% UCL of Mean 61.91701 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -49.78 18.63996 Unequal 7.19 -49.78 26.36088 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3523 Standard Error 11.78894 11.78894 95.0% LCL Difference -76.96535 -77.51069 95.0% UCL Difference -22.59465 -22.04931 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.2226 0.002906 Yes Difference < 0 -4.2226 0.001453 Yes Difference > 0 -4.2226 0.998547 No Difference: (Reach=R 03)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.957133 0.985695 0.000000 Power (Alpha=.010) 0.774334 0.874862 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.2226 0.003699 Yes Difference < 0 -4.2226 0.001849 Yes Difference > 0 -4.2226 0.998151 No Difference: (Reach=R 03)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.950963 0.983892 0.000000 Power (Alpha=.010) 0.744297 0.856739 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.0144 0.0007 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.514268 0.979362 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R15 Count 5 5 Median 25.7 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:05 AM 116 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 1 16 24 39 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.007937 Yes 0.992063 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.4023 0.016294 Yes -2.4023 0.008147 Yes -2.4023 0.991853 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.2978 0.021572 Yes -2.2978 0.010786 Yes -2.5067 0.993907 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:05 AM 117 Largemouth_Bass_PFOS_ppb vs Groups 110 n 20 Reach=R03 Reach=R15 Groups 5/1/2013 10:39:06 AM 118 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R05 5 404.88 192.5162 86.09584 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R05) = 2.7764 95.0% LCL of Mean 41.85218 165.8396 95.0% UCL of Mean 373.7478 643.9204 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -197.08 165.7176 Unequal 7.13 -197.08 234.3601 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3560 Standard Error 104.809 104.809 95.0% LCL Difference -438.7701 -444.0089 95.0% UCL Difference 44.61007 49.84889 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8804 0.096850 No Difference < 0 -1.8804 0.048425 Yes Difference > 0 -1.8804 0.951575 No Difference: (Reach=R04)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.380782 0.529521 0.000359 Power (Alpha=.010) 0.144648 0.226217 0.000040 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8804 0.101350 No Difference < 0 -1.8804 0.050675 No Difference > 0 -1.8804 0.949325 No Difference: (Reach=R04)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.370670 0.521629 0.000379 Power (Alpha=.010) 0.135780 0.215918 0.000044 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.0749 0.3556 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.496997 0.567468 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R05 Count 5 5 Median 159 435 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:06 AM 119 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R05 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 6 21 19 34 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.111111 No 0.888889 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.3578 0.174525 No -1.3578 0.087263 No -1.3578 0.912737 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.2534 0.210075 No -1.2534 0.105038 No -1.4623 0.928164 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:06 AM 120 5/1/2013 10:39:06 AM 121 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R06 5 65.46 32.25117 14.42316 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean 41.85218 25.41488 95.0% UCL of Mean 373.7478 105.5051 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 142.34 97.21712 Unequal 4.46 142.34 137.4858 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6662 Standard Error 61.48551 61.48551 95.0% LCL Difference 0.5541661 -21.59233 95.0% UCL Difference 284.1258 306.2723 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3150 0.049301 Yes Difference < 0 2.3150 0.975349 No Difference > 0 2.3150 0.024651 Yes Difference: (Reach=R04)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.529837 0.000076 0.680345 Power (Alpha=.010) 0.236605 0.000008 0.346903 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3150 0.074749 No Difference < 0 2.3150 0.962625 No Difference > 0 2.3150 0.037375 Yes Difference: (Reach=R04)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.444840 0.000116 0.620432 Power (Alpha=.010) 0.154169 0.000016 0.253720 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 17.1729 1.6460 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.017501 0.235425 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R06 Count 5 5 Median 159 50.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:06 AM 122 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 39 1 16 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.992063 No 0.007937 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.4023 0.016294 Yes 2.4023 0.991853 No 2.4023 0.008147 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.2978 0.021572 Yes 2.5067 0.993907 No 2.2978 0.010786 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:06 AM 123 Largemouth_Bass_PFOS_ppb vs Groups 450 n 0 J------------------- 1--------------------------------------- 1----- Reach=R04 Reach=R0B Groups 5/1/2013 10:39:06 AM 124 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R07 5 286.2 87.50543 39.13362 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 41.85218 177.5477 95.0% UCL of Mean 373.7478 394.8523 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -78.4 112.9588 Unequal 6.90 -78.4 159.7479 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3718 Standard Error 71.44144 71.44144 95.0% LCL Difference -243.1443 -247.8446 95.0% UCL Difference 86.34427 91.04461 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.0974 0.304395 No Difference < 0 -1.0974 0.152198 No Difference > 0 -1.0974 0.847802 No Difference: (Reach=R04)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.162791 0.261301 0.003943 Power (Alpha=.010) 0.046324 0.080509 0.000544 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.0974 0.309295 No Difference < 0 -1.0974 0.154647 No Difference > 0 -1.0974 0.845353 No Difference: (Reach=R04)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.158226 0.256650 0.004088 Power (Alpha=.010) 0.043668 0.076866 0.000585 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.3327 0.1606 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.432136 0.699069 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R07 Count 5 5 Median 159 262 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:06 AM 125 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 6 21 19 34 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.111111 No 0.888889 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.3578 0.174525 No -1.3578 0.087263 No -1.3578 0.912737 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.2534 0.210075 No -1.2534 0.105038 No -1.4623 0.928164 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:06 AM 126 5/1/2013 10:39:06 AM 127 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R08 5 102.24 51.72672 23.13289 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 41.85218 38.0128 95.0% UCL of Mean 373.7478 166.4672 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 105.56 101.3357 Unequal 5.17 105.56 143.3103 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5451 Standard Error 64.09033 64.09033 95.0% LCL Difference -42.23257 -57.55458 95.0% UCL Difference 253.3526 268.6746 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.6471 0.138163 No Difference < 0 1.6471 0.930919 No Difference > 0 1.6471 0.069081 No Difference: (Reach=R04)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.306146 0.000772 0.445049 Power (Alpha=.010) 0.106644 0.000091 0.172499 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.6471 0.158517 No Difference < 0 1.6471 0.920741 No Difference > 0 1.6471 0.079259 No Difference: (Reach=R04)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.271871 0.000939 0.415208 Power (Alpha=.010) 0.082394 0.000130 0.141759 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 6.6758 0.8391 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.092991 0.386444 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R08 Count 5 5 Median 159 80.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:07 AM 128 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 35 5 20 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.924603 No 0.075397 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.5667 0.117185 No 1.5667 0.941407 No 1.5667 0.058593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.4623 0.143672 No 1.6711 0.952654 No 1.4623 0.071836 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:07 AM 129 Largemouth_Bass_PFOS_ppb vs Groups 450 n 0 J------------------- 1--------------------------------------- 1----- Reach=R04 Reach=R0B Groups 5/1/2013 10:39:07 AM 130 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R09 5 231.8 57.10692 25.53899 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 41.85218 160.8924 95.0% UCL of Mean 373.7478 302.7076 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -24 102.7701 Unequal 5.41 -24 145.3389 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5126 Standard Error 64.99754 64.99754 95.0% LCL Difference -173.8846 -187.315 95.0% UCL Difference 125.8846 139.315 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.3692 0.721527 No Difference < 0 -0.3692 0.360764 No Difference > 0 -0.3692 0.639236 No Difference: (Reach=R04)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.062349 0.095811 0.023591 Power (Alpha=.010) 0.013482 0.022032 0.004133 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.3692 0.725952 No Difference < 0 -0.3692 0.362976 No Difference > 0 -0.3692 0.637024 No Difference: (Reach=R04)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.060999 0.093536 0.024307 Power (Alpha=.010) 0.012893 0.020878 0.004395 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.4772 0.6113 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.128294 0.456823 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R09 Count 5 5 Median 159 259 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:07 AM 131 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 7 22 18 33 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.154762 No 0.845238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.1489 0.250592 No -1.1489 0.125296 No -1.1489 0.874704 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.0445 0.296270 No -1.0445 0.148135 No -1.2534 0.894962 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:07 AM 132 5/1/2013 10:39:07 AM 133 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R10 5 133.62 61.49823 27.50284 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 41.85218 57.25986 95.0% UCL of Mean 373.7478 209.9801 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 74.18 104.0294 Unequal 5.62 74.18 147.1198 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4874 Standard Error 65.79397 65.79397 95.0% LCL Difference -77.54117 -89.47884 95.0% UCL Difference 225.9012 237.8389 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1275 0.292226 No Difference < 0 1.1275 0.853887 No Difference > 0 1.1275 0.146113 No Difference: (Reach=R 04)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.169199 0.003630 0.270305 Power (Alpha=.010) 0.048683 0.000496 0.084326 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1275 0.305362 No Difference < 0 1.1275 0.847319 No Difference > 0 1.1275 0.152681 No Difference: (Reach=R 04)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.156822 0.003996 0.257611 Power (Alpha=.010) 0.041695 0.000598 0.074614 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.7229 0.5349 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.161856 0.485415 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R10 Count 5 5 Median 159 104 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:07 AM 134 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R10 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 18.5 33.5 6.5 21.5 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.2572 0.208690 No 1.2572 0.895655 No 1.2572 0.104345 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.1524 0.249153 No 1.3619 0.913391 No 1.1524 0.124576 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.000000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:07 AM 135 Largemouth_Bass_PFOS_ppb vs Groups 450 n 350 50 J-----------------------------1---------------------------------------------------------- T-------- Reach=R04 Reach=R10 Groups 5/1/2013 10:39:07 AM 136 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 41.85218 97.42249 95.0% UCL of Mean 373.7478 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 45.6 101.4492 Unequal 5.19 45.6 143.4709 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5423 Standard Error 64.16214 64.16214 95.0% LCL Difference -102.3582 -117.5225 95.0% UCL Difference 193.5582 208.7225 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7107 0.497464 No Difference < 0 0.7107 0.751268 No Difference > 0 0.7107 0.248732 No Difference: (Reach=R04)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.096432 0.010737 0.160376 Power (Alpha=.010) 0.023740 0.001676 0.042199 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7107 0.507919 No Difference < 0 0.7107 0.746040 No Difference > 0 0.7107 0.253960 No Difference: (Reach=R04)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.090570 0.011518 0.153138 Power (Alpha=.010) 0.020967 0.001925 0.037870 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 6.5629 0.7527 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.095653 0.410899 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R11 Count 5 5 Median 159 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:07 AM 137 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 14 29 11 26 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.841270 No 0.579365 No 0.420635 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.3133 0.754023 No 0.3133 0.622989 No 0.3133 0.377011 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.2089 0.834532 No 0.4178 0.661948 No 0.2089 0.417266 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.200000 0.200000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 1.0000 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:08 AM 138 5/1/2013 10:39:08 AM 139 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 41.85218 49.75645 95.0% UCL of Mean 373.7478 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 137.68 95.21336 Unequal 4.12 137.68 134.652 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7447 Standard Error 60.21822 60.21822 95.0% LCL Difference -1.183452 -27.60281 95.0% UCL Difference 276.5435 302.9628 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2864 0.051558 No Difference < 0 2.2864 0.974221 No Difference > 0 2.2864 0.025779 Yes Difference: (Reach=R04)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.519918 0.000085 0.670998 Power (Alpha=.010) 0.229722 0.000008 0.338262 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2864 0.082256 No Difference < 0 2.2864 0.958872 No Difference > 0 2.2864 0.041128 Yes Difference: (Reach=R04)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.421398 0.000137 0.599945 Power (Alpha=.010) 0.138826 0.000019 0.233398 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 66.4104 2.3928 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001307 0.160478 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R12 Count 5 5 Median 159 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:08 AM 140 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:08 AM 141 5/1/2013 10:39:08 AM 142 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (R e ac h =R04) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 41.85218 60.96663 95.0% UCL of Mean 373.7478 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 130.78 94.94566 Unequal 4.07 130.78 134.2734 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7564 Standard Error 60.04891 60.04891 95.0% LCL Difference -7.693031 -34.7415 95.0% UCL Difference 269.253 296.3015 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1779 0.061063 No Difference < 0 2.1779 0.969469 No Difference > 0 2.1779 0.030531 Yes Difference: (Reach=R 04)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.482309 0.000126 0.634683 Power (Alpha=.010) 0.204689 0.000013 0.306329 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1779 0.093715 No Difference < 0 2.1779 0.953142 No Difference > 0 2.1779 0.046858 Yes Difference: (Reach=R 04)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.388272 0.000200 0.564060 Power (Alpha=.010) 0.123444 0.000029 0.210284 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 106.8588 2.4361 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000513 0.157196 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R13 Count 5 5 Median 159 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:08 AM 143 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:08 AM 144 450 n Largemouth_Bass_PFOS_ppb vs Groups 350 50 J-----------------------------1---------------------------------------------------------- T-------- Reach=R04 Reach=R13 Groups 5/1/2013 10:39:08 AM 145 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 41.85218 50.37198 95.0% UCL of Mean 373.7478 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 134.48 95.40381 Unequal 4.15 134.48 134.9214 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7366 Standard Error 60.33867 60.33867 95.0% LCL Difference -4.661209 -30.64191 95.0% UCL Difference 273.6212 299.6019 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2288 0.056407 No Difference < 0 2.2288 0.971797 No Difference > 0 2.2288 0.028203 Yes Difference: (Reach=R04)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.499951 0.000105 0.651890 Power (Alpha=.010) 0.216227 0.000011 0.321146 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2288 0.087255 No Difference < 0 2.2288 0.956373 No Difference > 0 2.2288 0.043627 Yes Difference: (Reach=R04)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.406218 0.000166 0.582966 Power (Alpha=.010) 0.132252 0.000023 0.223234 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 52.2941 2.0601 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.002086 0.189118 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R14 Count 5 5 Median 159 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:08 AM 146 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:09 AM 147 Largemouth_Bass_PFOS_ppb vs Groups 450 n 5/1/2013 10:39:09 AM 148 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 207.8 133.6495 59.76989 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 41.85218 66.08784 95.0% UCL of Mean 373.7478 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 122.86 95.11237 Unequal 4.10 122.86 134.5092 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7491 Standard Error 60.15434 60.15434 95.0% LCL Difference -15.85616 -42.51113 95.0% UCL Difference 261.5761 288.2311 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0424 0.075391 No Difference < 0 2.0424 0.962305 No Difference > 0 2.0424 0.037695 Yes Difference: (Reach=R 04)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.435547 0.000205 0.587568 Power (Alpha=.010) 0.175748 0.000022 0.268371 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0424 0.108877 No Difference < 0 2.0424 0.945562 No Difference > 0 2.0424 0.054438 No Difference: (Reach=R 04)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.351506 0.000312 0.521479 Power (Alpha=.010) 0.108131 0.000045 0.186325 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 77.4855 2.2548 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000966 0.171597 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R15 Count 5 5 Median 159 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:09 AM 149 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:09 AM 150 450 n Largemouth_Bass_PFOS_ppb vs Groups 350 - 50 J-----------------------------1---------------------------------------------------------- T-------- Reach=R04 Reach=R15 Groups 5/1/2013 10:39:09 AM 151 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R06 5 65.46 32.25117 14.42316 Note: T-alpha (Reach:=R05) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean 165.8396 25.41488 95.0% UCL of Mean 643.9204 105.5051 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 339.42 138.0265 Unequal 4.22 339.42 195.1989 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7193 Standard Error 87.2956 87.2956 95.0% LCL Difference 138.116 102.0414 95.0% UCL Difference 540.724 576.7986 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.8882 0.004621 Yes Difference < 0 3.8882 0.997689 No Difference > 0 3.8882 0.002311 Yes Difference: (Reach=R05)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.923961 0.000000 0.970527 Power (Alpha=.010) 0.691113 0.000000 0.811125 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.8882 0.015972 Yes Difference < 0 3.8882 0.992014 No Difference > 0 3.8882 0.007986 Yes Difference: (Reach=R05)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.836904 0.000000 0.939045 Power (Alpha=.010) 0.448375 0.000000 0.624576 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 35.6323 3.3835 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.004390 0.103137 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R06 Count 5 5 Median 435 50.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:09 AM 152 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R06 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 39 1 16 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.992063 No 0.007937 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.4023 0.016294 Yes 2.4023 0.991853 No 2.4023 0.008147 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.2978 0.021572 Yes 2.5067 0.993907 No 2.2978 0.010786 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:09 AM 153 Largemouth_Bass_PFOS_ppb vs Groups Reach=R05 Groups Reach=R0B 5/1/2013 10:39:09 AM 154 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R07 5 286.2 87.50543 39.13362 Note: T-alpha (Reach:=R05) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 165.8396 177.5477 95.0% UCL of Mean 643.9204 394.8523 Confidence-Limits of Difference Section Variance Assumption DF Equal 8 Unequal 5.59 Note: T-alpha (Equal) = 2.3060, Mean Standard Difference Deviation 118.68 149.5321 118.68 211.4703 T-alpha (Unequal) = 2.4917 Standard Error 94.57238 94.57238 95.0% LCL Difference -99.40429 -116.9613 95.0% UCL Difference 336.7643 354.3213 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.2549 0.244929 No Difference < 0 1.2549 0.877536 No Difference > 0 1.2549 0.122464 No Difference: (Reach=R05)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.198323 0.002534 0.310104 Power (Alpha=.010) 0.059797 0.000334 0.102005 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.2549 0.259463 No Difference < 0 1.2549 0.870268 No Difference > 0 1.2549 0.129732 No Difference: (Reach=R05)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.182493 0.002834 0.294552 Power (Alpha=.010) 0.050348 0.000413 0.089197 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.8402 1.1107 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.155831 0.322721 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R07 Count 5 5 Median 435 262 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:09 AM 155 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 19 34 6 21 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.888889 No 0.111111 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.3578 0.174525 No 1.3578 0.912737 No 1.3578 0.087263 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.2534 0.210075 No 1.4623 0.928164 No 1.2534 0.105038 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.200000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:10 AM 156 5/1/2013 10:39:10 AM 157 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R08 5 102.24 51.72672 23.13289 Note: T-alpha (Reach:=R05) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 165.8396 38.0128 95.0% UCL of Mean 643.9204 166.4672 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 302.64 140.9577 Unequal 4.57 302.64 199.3442 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6442 Standard Error 89.14945 89.14945 95.0% LCL Difference 97.06099 66.91266 95.0% UCL Difference 508.219 538.3673 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.3947 0.009433 Yes Difference < 0 3.3947 0.995283 No Difference > 0 3.3947 0.004717 Yes Difference: (Reach=R05)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.843408 0.000001 0.925715 Power (Alpha=.010) 0.548264 0.000000 0.686152 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.3947 0.022241 Yes Difference < 0 3.3947 0.988880 No Difference > 0 3.3947 0.011120 Yes Difference: (Reach=R05)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.754177 0.000002 0.885401 Power (Alpha=.010) 0.369393 0.000000 0.531640 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 13.8517 2.3151 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.025981 0.166621 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R08 Count 5 5 Median 435 80.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:10 AM 158 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 23 38 2 17 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.984127 No 0.015873 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.1934 0.028280 Yes 2.1934 0.985860 No 2.1934 0.014140 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.0889 0.036714 Yes 2.2978 0.989214 No 2.0889 0.018357 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:10 AM 159 5/1/2013 10:39:10 AM 160 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R09 5 231.8 57.10692 25.53899 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 165.8396 160.8924 95.0% UCL of Mean 643.9204 302.7076 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 173.08 141.9924 Unequal 4.70 173.08 200.8076 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6210 Standard Error 89.80386 89.80386 95.0% LCL Difference -34.00809 -62.29523 95.0% UCL Difference 380.1681 408.4552 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.9273 0.090094 No Difference < 0 1.9273 0.954953 No Difference > 0 1.9273 0.045047 Yes Difference: (Reach=R05)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.396459 0.000306 0.546462 Power (Alpha=.010) 0.153259 0.000033 0.238039 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.9273 0.115572 No Difference < 0 1.9273 0.942214 No Difference > 0 1.9273 0.057786 No Difference: (Reach=R05)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.338715 0.000413 0.500139 Power (Alpha=.010) 0.107355 0.000057 0.182248 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 11.3647 1.9727 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.037129 0.197779 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R09 Count 5 5 Median 435 259 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:10 AM 161 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 35 5 20 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.924603 No 0.075397 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.5667 0.117185 No 1.5667 0.941407 No 1.5667 0.058593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.4623 0.143672 No 1.6711 0.952654 No 1.4623 0.071836 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.200000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:10 AM 162 5/1/2013 10:39:10 AM 163 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R10 5 133.62 61.49823 27.50284 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 165.8396 57.25986 95.0% UCL of Mean 643.9204 209.9801 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 271.26 142.9064 Unequal 4.81 271.26 202.1002 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6018 Standard Error 90.38197 90.38197 95.0% LCL Difference 62.8388 36.10638 95.0% UCL Difference 479.6812 506.4136 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.0013 0.017039 Yes Difference < 0 3.0013 0.991481 No Difference > 0 3.0013 0.008519 Yes Difference: (Reach=R 05)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.748365 0.000005 0.862065 Power (Alpha=.010) 0.427649 0.000000 0.566057 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.0013 0.031575 Yes Difference < 0 3.0013 0.984212 No Difference > 0 3.0013 0.015788 Yes Difference: (Reach=R 05)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.664125 0.000008 0.817188 Power (Alpha=.010) 0.296918 0.000001 0.442168 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 9.7996 1.8371 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.048268 0.212317 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R10 Count 5 5 Median 435 104 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:10 AM 164 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 21 36 4 19 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.952381 No 0.047619 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.7756 0.075800 No 1.7756 0.962100 No 1.7756 0.037900 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.6711 0.094693 No 1.8800 0.969949 No 1.6711 0.047346 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:10 AM 165 5/1/2013 10:39:11 AM 166 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 165.8396 97.42249 95.0% UCL of Mean 643.9204 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 242.68 141.0393 Unequal 4.58 242.68 199.4597 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6423 Standard Error 89.20109 89.20109 95.0% LCL Difference 36.98192 6.984518 95.0% UCL Difference 448.3781 478.3755 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7206 0.026225 Yes Difference < 0 2.7206 0.986888 No Difference > 0 2.7206 0.013112 Yes Difference: (Reach=R05)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.665185 0.000016 0.798414 Power (Alpha=.010) 0.344469 0.000001 0.475271 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7206 0.045749 Yes Difference < 0 2.7206 0.977126 No Difference > 0 2.7206 0.022874 Yes Difference: (Reach=R05)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.572423 0.000025 0.742607 Power (Alpha=.010) 0.227700 0.000003 0.355491 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 13.6174 2.2108 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.026800 0.175357 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R11 Count 5 5 Median 435 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:11 AM 167 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 35 5 20 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.924603 No 0.075397 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.5667 0.117185 No 1.5667 0.941407 No 1.5667 0.058593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.4623 0.143672 No 1.6711 0.952654 No 1.4623 0.071836 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.200000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:11 AM 168 5/1/2013 10:39:11 AM 169 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 165.8396 49.75645 95.0% UCL of Mean 643.9204 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 334.76 136.6225 Unequal 4.06 334.76 193.2135 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7609 Standard Error 86.40768 86.40768 95.0% LCL Difference 135.5035 96.2013 95.0% UCL Difference 534.0165 573.3187 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.8742 0.004713 Yes Difference < 0 3.8742 0.997643 No Difference > 0 3.8742 0.002357 Yes Difference: (Reach=R05)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.922233 0.000000 0.969675 Power (Alpha=.010) 0.687351 0.000000 0.808079 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.8742 0.017449 Yes Difference < 0 3.8742 0.991276 No Difference > 0 3.8742 0.008724 Yes Difference: (Reach=R05)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.824933 0.000000 0.933976 Power (Alpha=.010) 0.426649 0.000000 0.604291 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 137.7956 4.2665 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000310 0.072730 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R12 Count 5 5 Median 435 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:11 AM 170 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 39 1 16 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.992063 No 0.007937 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.4023 0.016294 Yes 2.4023 0.991853 No 2.4023 0.008147 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.2978 0.021572 Yes 2.5067 0.993907 No 2.2978 0.010786 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:11 AM 171 5/1/2013 10:39:11 AM 172 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 165.8396 60.96663 95.0% UCL of Mean 643.9204 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 327.86 136.4361 Unequal 4.04 327.86 192.9498 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7667 Standard Error 86.28978 86.28978 95.0% LCL Difference 128.8754 89.12346 95.0% UCL Difference 526.8446 566.5966 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.7995 0.005240 Yes Difference < 0 3.7995 0.997380 No Difference > 0 3.7995 0.002620 Yes Difference: (Reach=R 05)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.912490 0.000000 0.964768 Power (Alpha=.010) 0.666907 0.000000 0.791291 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.7995 0.018799 Yes Difference < 0 3.7995 0.990600 No Difference > 0 3.7995 0.009400 Yes Difference: (Reach=R 05)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.809722 0.000000 0.925598 Power (Alpha=.010) 0.408157 0.000000 0.584338 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 221.7225 4.3165 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000121 0.071380 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R13 Count 5 5 Median 435 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:11 AM 173 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 39 1 16 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.992063 No 0.007937 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.4023 0.016294 Yes 2.4023 0.991853 No 2.4023 0.008147 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.2978 0.021572 Yes 2.5067 0.993907 No 2.2978 0.010786 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:11 AM 174 5/1/2013 10:39:12 AM 175 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 165.8396 50.37198 95.0% UCL of Mean 643.9204 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 331.56 136.7553 Unequal 4.07 331.56 193.4012 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7567 Standard Error 86.49167 86.49167 95.0% LCL Difference 132.1098 93.12487 95.0% UCL Difference 531.0101 569.9951 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.8334 0.004994 Yes Difference < 0 3.8334 0.997503 No Difference > 0 3.8334 0.002497 Yes Difference: (Reach=R05)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.917023 0.000000 0.967072 Power (Alpha=.010) 0.676261 0.000000 0.799022 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.8334 0.017944 Yes Difference < 0 3.8334 0.991028 No Difference > 0 3.8334 0.008972 Yes Difference: (Reach=R05)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.818412 0.000000 0.930178 Power (Alpha=.010) 0.419641 0.000000 0.596333 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 108.5056 3.8932 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000497 0.083936 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R14 Count 5 5 Median 435 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:12 AM 176 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:12 AM 177 600 490 _QQ_ a. cn o 380 C.LI 2 270 5p3 iTj 160 50 Reach=R05 Groups Reach=R14 5/1/2013 10:39:12 AM 178 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 404.88 192.5162 86.09584 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 165.8396 66.08784 95.0% UCL of Mean 643.9204 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 319.94 136.5522 Unequal 4.05 319.94 193.1139 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7630 Standard Error 86.36318 86.36318 95.0% LCL Difference 120.7861 81.31478 95.0% UCL Difference 519.0939 558.5652 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.7046 0.006003 Yes Difference < 0 3.7046 0.996999 No Difference > 0 3.7046 0.003001 Yes Difference: (Reach=R 05)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.898813 0.000000 0.957605 Power (Alpha=.010) 0.640148 0.000000 0.768717 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.7046 0.020303 Yes Difference < 0 3.7046 0.989849 No Difference > 0 3.7046 0.010151 Yes Difference: (Reach=R 05)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.791968 0.000000 0.914977 Power (Alpha=.010) 0.389452 0.000000 0.563033 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 160.7756 4.1149 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000228 0.077034 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R15 Count 5 5 Median 435 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:12 AM 179 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 39 1 16 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.992063 No 0.007937 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.4023 0.016294 Yes 2.4023 0.991853 No 2.4023 0.008147 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.2978 0.021572 Yes 2.5067 0.993907 No 2.2978 0.010786 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:12 AM 180 5/1/2013 10:39:12 AM 181 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 65.46 32.25117 14.42316 Reach=R07 5 286.2 87.50543 39.13362 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 25.41488 177.5477 95.0% UCL of Mean 105.5051 394.8523 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -220.74 65.94444 Unequal 5.07 -220.74 93.25952 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5604 Standard Error 41.70692 41.70692 95.0% LCL Difference -316.9164 -327.526 95.0% UCL Difference -124.5637 -113.954 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.2926 0.000735 Yes Difference < 0 -5.2926 0.000367 Yes Difference > 0 -5.2926 0.999633 No Difference: (Reach=R06)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.995710 0.999172 0.000000 Power (Alpha=.010) 0.940290 0.977158 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.2926 0.003086 Yes Difference < 0 -5.2926 0.001543 Yes Difference > 0 -5.2926 0.998457 No Difference: (Reach=R06)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.985977 0.997619 0.000000 Power (Alpha=.010) 0.825820 0.926232 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 7.3617 2.0875 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.078973 0.186513 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R07 Count 5 5 Median 50.6 262 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:12 AM 182 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:12 AM 183 5/1/2013 10:39:13 AM 184 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 65.46 32.25117 14.42316 Reach=R08 5 102.24 51.72672 23.13289 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 25.41488 38.0128 95.0% UCL of Mean 105.5051 166.4672 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -36.78 43.10331 Unequal 6.70 -36.78 60.95729 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3861 Standard Error 27.26093 27.26093 95.0% LCL Difference -99.64381 -101.8282 95.0% UCL Difference 26.08381 28.26818 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.3492 0.214218 No Difference < 0 -1.3492 0.107109 No Difference > 0 -1.3492 0.892891 No Difference: (Reach=R06)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.221841 0.341062 0.001926 Power (Alpha=.010) 0.069243 0.116691 0.000247 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.3492 0.221079 No Difference < 0 -1.3492 0.110540 No Difference > 0 -1.3492 0.889460 No Difference: (Reach=R06)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.213360 0.333118 0.002038 Power (Alpha=.010) 0.063719 0.109481 0.000275 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.5724 0.6319 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.382407 0.449608 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R08 Count 5 5 Median 50.6 80.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:13 AM 185 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 6 21 19 34 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.111111 No 0.888889 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.3578 0.174525 No -1.3578 0.087263 No -1.3578 0.912737 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.2534 0.210075 No -1.2534 0.105038 No -1.4623 0.928164 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:13 AM 186 5/1/2013 10:39:13 AM 187 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 65.46 32.25117 14.42316 Reach=R09 5 231.8 57.10692 25.53899 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 25.41488 160.8924 95.0% UCL of Mean 105.5051 302.7076 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -166.34 46.37531 Unequal 6.32 -166.34 65.58459 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4175 Standard Error 29.33032 29.33032 95.0% LCL Difference -233.9758 -237.2473 95.0% UCL Difference -98.70416 -95.43266 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.6713 0.000470 Yes Difference < 0 -5.6713 0.000235 Yes Difference > 0 -5.6713 0.999765 No Difference: (Reach=R06)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.998404 0.999752 0.000000 Power (Alpha=.010) 0.967010 0.989161 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.6713 0.001085 Yes Difference < 0 -5.6713 0.000542 Yes Difference > 0 -5.6713 0.999458 No Difference: (Reach=R06)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996845 0.999554 0.000000 Power (Alpha=.010) 0.935326 0.977995 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.1354 1.0969 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.294292 0.325563 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R09 Count 5 5 Median 50.6 259 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:13 AM 188 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:13 AM 189 5/1/2013 10:39:13 AM 190 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 65.46 32.25117 14.42316 Reach=R10 5 133.62 61.49823 27.50284 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 25.41488 57.25986 95.0% UCL of Mean 105.5051 209.9801 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -68.16 49.1028 Unequal 6.05 -68.16 69.44185 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4425 Standard Error 31.05534 31.05534 95.0% LCL Difference -139.7737 -144.0114 95.0% UCL Difference 3.453737 7.691386 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.1948 0.059475 No Difference < 0 -2.1948 0.029737 Yes Difference > 0 -2.1948 0.970263 No Difference: (Reach=R 06)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.488170 0.640433 0.000119 Power (Alpha=.010) 0.208483 0.311220 0.000012 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.1948 0.070270 No Difference < 0 -2.1948 0.035135 Yes Difference > 0 -2.1948 0.964865 No Difference: (Reach=R 06)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.454922 0.616847 0.000141 Power (Alpha=.010) 0.175002 0.274190 0.000017 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.6361 1.0883 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.239014 0.327334 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R10 Count 5 5 Median 50.6 104 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:13 AM 191 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 4 19 21 36 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.047619 Yes 0.952381 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.7756 0.075800 No -1.7756 0.037900 Yes -1.7756 0.962100 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.6711 0.094693 No -1.6711 0.047346 Yes -1.8800 0.969949 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:13 AM 192 220n Largemouth_Bass_PFOS_ppb vs Groups Reach=R0B Groups Reach=R10 5/1/2013 10:39:14 AM 193 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 65.46 32.25117 14.42316 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 25.41488 97.42249 95.0% UCL of Mean 105.5051 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -96.74 43.36956 Unequal 6.67 -96.74 61.33382 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3887 Standard Error 27.42932 27.42932 95.0% LCL Difference -159.9921 -162.2611 95.0% UCL Difference -33.48787 -31.2189 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.5269 0.007767 Yes Difference < 0 -3.5269 0.003884 Yes Difference > 0 -3.5269 0.996116 No Difference: (Reach=R06)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.869111 0.941046 0.000000 Power (Alpha=.010) 0.588137 0.722931 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.5269 0.010435 Yes Difference < 0 -3.5269 0.005218 Yes Difference > 0 -3.5269 0.994782 No Difference: (Reach=R06)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.848819 0.932740 0.000001 Power (Alpha=.010) 0.534723 0.682004 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.6167 1.1630 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.374151 0.312280 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R11 Count 5 5 Median 50.6 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:14 AM 194 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 2 17 23 38 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.015873 Yes 0.984127 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.1934 0.028280 Yes -2.1934 0.014140 Yes -2.1934 0.985860 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.0889 0.036714 Yes -2.0889 0.018357 Yes -2.2978 0.989214 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:14 AM 195 5/1/2013 10:39:14 AM 196 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 65.46 32.25117 14.42316 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 25.41488 49.75645 95.0% UCL of Mean 105.5051 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -4.66 25.58422 Unequal 5.94 -4.66 36.18156 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4530 Standard Error 16.18088 16.18088 95.0% LCL Difference -41.97319 -44.35191 95.0% UCL Difference 32.65319 35.03191 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.2880 0.780671 No Difference < 0 -0.2880 0.390336 No Difference > 0 -0.2880 0.609664 No Difference: (Reach=R06)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.057494 0.083741 0.028078 Power (Alpha=.010) 0.012097 0.018663 0.005061 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.2880 0.783137 No Difference < 0 -0.2880 0.391568 No Difference > 0 -0.2880 0.608432 No Difference: (Reach=R06)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.056891 0.082570 0.028552 Power (Alpha=.010) 0.011834 0.018084 0.005242 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.8672 0.6496 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.218587 0.443552 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R12 Count 5 5 Median 50.6 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:14 AM 197 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 9 24 16 31 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.273810 No 0.726191 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.7311 0.464702 No -0.7311 0.232351 No -0.7311 0.767649 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.6267 0.530869 No -0.6267 0.265435 No -0.8356 0.798302 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:14 AM 198 5/1/2013 10:39:14 AM 199 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 65.46 32.25117 14.42316 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 25.41488 60.96663 95.0% UCL of Mean 105.5051 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -11.56 24.56924 Unequal 5.25 -11.56 34.74615 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5337 Standard Error 15.53895 15.53895 95.0% LCL Difference -47.39289 -50.93177 95.0% UCL Difference 24.27289 27.81178 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.7439 0.478193 No Difference < 0 -0.7439 0.239097 No Difference > 0 -0.7439 0.760903 No Difference: (Reach=R 06)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.100961 0.167902 0.009897 Power (Alpha=.010) 0.025173 0.044774 0.001528 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.7439 0.488835 No Difference < 0 -0.7439 0.244417 No Difference > 0 -0.7439 0.755583 No Difference: (Reach=R 06)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.094710 0.160330 0.010632 Power (Alpha=.010) 0.022176 0.040155 0.001760 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 6.2225 0.7562 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.104403 0.409853 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R13 Count 5 5 Median 50.6 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:14 AM 200 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 7 22 18 33 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.154762 No 0.845238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.1489 0.250592 No -1.1489 0.125296 No -1.1489 0.874704 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.0445 0.296270 No -1.0445 0.148135 No -1.2534 0.894962 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:14 AM 201 12 n Largemouth_Bass_PFOS_ppb vs Groups ----- ----- 5/1/2013 10:39:14 AM 202 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 65.46 32.25117 14.42316 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 25.41488 50.37198 95.0% UCL of Mean 105.5051 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -7.86 26.28412 Unequal 6.37 -7.86 37.17136 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4127 Standard Error 16.62354 16.62354 95.0% LCL Difference -46.19395 -47.96843 95.0% UCL Difference 30.47395 32.24843 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.4728 0.648970 No Difference < 0 -0.4728 0.324485 No Difference > 0 -0.4728 0.675515 No Difference: (Reach=R06)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.070328 0.112983 0.018758 Power (Alpha=.010) 0.015798 0.027051 0.003171 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.4728 0.652104 No Difference < 0 -0.4728 0.326052 No Difference > 0 -0.4728 0.673948 No Difference: (Reach=R06)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.069094 0.111186 0.019164 Power (Alpha=.010) 0.015233 0.026066 0.003315 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.0452 0.1758 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.306245 0.686075 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R14 Count 5 5 Median 50.6 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:14 AM 203 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 7 22 18 33 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.154762 No 0.845238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.1489 0.250592 No -1.1489 0.125296 No -1.1489 0.874704 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.0445 0.296270 No -1.0445 0.148135 No -1.2534 0.894962 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:15 AM 204 12 n Largemouth_Bass_PFOS_ppb vs Groups ----- ----- 5/1/2013 10:39:15 AM 205 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 65.46 32.25117 14.42316 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 25.41488 66.08784 95.0% UCL of Mean 105.5051 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -19.48 25.20576 Unequal 5.69 -19.48 35.64633 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4796 Standard Error 15.94152 15.94152 95.0% LCL Difference -56.24122 -59.00848 95.0% UCL Difference 17.28122 20.04848 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.2220 0.256502 No Difference < 0 -1.2220 0.128251 No Difference > 0 -1.2220 0.871749 No Difference: (Reach=R 06)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.190495 0.299576 0.002784 Power (Alpha=.010) 0.056747 0.097199 0.000370 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.2220 0.269915 No Difference < 0 -1.2220 0.134958 No Difference > 0 -1.2220 0.865042 No Difference: (Reach=R 06)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.176393 0.285592 0.003083 Power (Alpha=.010) 0.048418 0.085858 0.000450 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.5121 0.4412 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.173594 0.525232 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R15 Count 5 5 Median 50.6 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:15 AM 206 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 6 21 19 34 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.111111 No 0.888889 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.3578 0.174525 No -1.3578 0.087263 No -1.3578 0.912737 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.2534 0.210075 No -1.2534 0.105038 No -1.4623 0.928164 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:15 AM 207 12 n Largemouth_Bass_PFOS_ppb vs Groups ----- ----- 5/1/2013 10:39:15 AM 208 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 286.2 87.50543 39.13362 Reach=R08 5 102.24 51.72672 23.13289 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 177.5477 38.0128 95.0% UCL of Mean 394.8523 166.4672 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 183.96 71.87786 Unequal 6.49 183.96 101.6506 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4027 Standard Error 45.45955 45.45955 95.0% LCL Difference 79.13009 74.73357 95.0% UCL Difference 288.7899 293.1864 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 4.0467 0.003701 Yes Difference < 0 4.0467 0.998149 No Difference > 0 4.0467 0.001851 Yes Difference: (Reach=R07)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.941552 0.000000 0.978870 Power (Alpha=.010) 0.732260 0.000000 0.843523 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 4.0467 0.005728 Yes Difference < 0 4.0467 0.997136 No Difference > 0 4.0467 0.002864 Yes Difference: (Reach=R07)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.925506 0.000000 0.973735 Power (Alpha=.010) 0.667771 0.000000 0.801416 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.8618 0.6318 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.332866 0.449639 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R08 Count 5 5 Median 262 80.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:15 AM 209 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:15 AM 210 450 n Largemouth_Bass_PFOS_ppb vs Groups 0 J------------------- 1--------------------------------------- 1----- Reach=R07 Reach=R0B Groups 5/1/2013 10:39:15 AM 211 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 286.2 87.50543 39.13362 Reach=R09 5 231.8 57.10692 25.53899 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 177.5477 160.8924 95.0% UCL of Mean 394.8523 302.7076 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 54.4 73.8864 Unequal 6.88 54.4 104.4911 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3727 Standard Error 46.72986 46.72986 95.0% LCL Difference -53.35926 -56.47695 95.0% UCL Difference 162.1593 165.2769 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1641 0.277909 No Difference < 0 1.1641 0.861046 No Difference > 0 1.1641 0.138954 No Difference: (Reach=R07)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.177258 0.003277 0.281496 Power (Alpha=.010) 0.051694 0.000443 0.089163 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1641 0.283122 No Difference < 0 1.1641 0.858439 No Difference > 0 1.1641 0.141561 No Difference: (Reach=R07)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.172019 0.003409 0.276282 Power (Alpha=.010) 0.048552 0.000479 0.084915 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.3480 0.3291 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.428699 0.581977 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R09 Count 5 5 Median 262 259 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:15 AM 212 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 17 32 8 23 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.789683 No 0.210318 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.9400 0.347208 No 0.9400 0.826396 No 0.9400 0.173604 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.8356 0.403395 No 1.0445 0.851865 No 0.8356 0.201698 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.000000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:15 AM 213 420 n Largemouth_Bass_PFOS_ppb vs Groups 160 Reach=R07 Reach=R09 Groups 5/1/2013 10:39:16 AM 214 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 286.2 87.50543 39.13362 Reach=R10 5 133.62 61.49823 27.50284 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 177.5477 57.25986 95.0% UCL of Mean 394.8523 209.9801 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 152.58 75.62814 Unequal 7.18 152.58 106.9543 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3529 Standard Error 47.83144 47.83144 95.0% LCL Difference 42.28051 40.0379 95.0% UCL Difference 262.8795 265.1221 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.1900 0.012804 Yes Difference < 0 3.1900 0.993598 No Difference > 0 3.1900 0.006402 Yes Difference: (Reach=R 07)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.797343 0.000002 0.896158 Power (Alpha=.010) 0.485448 0.000000 0.625273 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.1900 0.014777 Yes Difference < 0 3.1900 0.992611 No Difference > 0 3.1900 0.007389 Yes Difference: (Reach=R 07)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.784031 0.000002 0.889866 Power (Alpha=.010) 0.457759 0.000000 0.602131 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.0246 0.2412 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.511296 0.636552 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R10 Count 5 5 Median 262 104 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:16 AM 215 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 23 38 2 17 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.984127 No 0.015873 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.1934 0.028280 Yes 2.1934 0.985860 No 2.1934 0.014140 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.0889 0.036714 Yes 2.2978 0.989214 No 2.0889 0.018357 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:16 AM 216 450 n Largemouth_Bass_PFOS_ppb vs Groups 50 J--------------------------1-----------------------------------------------------T------- Reach=R07 Reach=R10 Groups 5/1/2013 10:39:16 AM 217 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 286.2 87.50543 39.13362 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 177.5477 97.42249 95.0% UCL of Mean 394.8523 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 124 72.03783 Unequal 6.52 124 101.8769 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4000 Standard Error 45.56073 45.56073 95.0% LCL Difference 18.93677 14.65405 95.0% UCL Difference 229.0632 233.3459 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7216 0.026182 Yes Difference < 0 2.7216 0.986909 No Difference > 0 2.7216 0.013091 Yes Difference: (Reach=R07)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.665515 0.000015 0.798680 Power (Alpha=.010) 0.344770 0.000001 0.475611 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7216 0.031800 Yes Difference < 0 2.7216 0.984100 No Difference > 0 2.7216 0.015900 Yes Difference: (Reach=R07)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.637639 0.000018 0.782588 Power (Alpha=.010) 0.304482 0.000002 0.436815 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.8134 0.5279 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.340468 0.488215 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R11 Count 5 5 Median 262 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:16 AM 218 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 23 38 2 17 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.984127 No 0.015873 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.1934 0.028280 Yes 2.1934 0.985860 No 2.1934 0.014140 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.0889 0.036714 Yes 2.2978 0.989214 No 2.0889 0.018357 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:16 AM 219 5/1/2013 10:39:16 AM 220 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 286.2 87.50543 39.13362 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (R e ac h =R07) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 177.5477 49.75645 95.0% UCL of Mean 394.8523 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 216.08 62.95303 Unequal 4.28 216.08 89.02902 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7061 Standard Error 39.81499 39.81499 95.0% LCL Difference 124.2665 108.3364 95.0% UCL Difference 307.8935 323.8236 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.4271 0.000625 Yes Difference < 0 5.4271 0.999687 No Difference > 0 5.4271 0.000313 Yes Difference: (Reach=R07)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996948 0.000000 0.999454 Power (Alpha=.010) 0.951271 0.000000 0.982317 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.4271 0.004591 Yes Difference < 0 5.4271 0.997704 No Difference > 0 5.4271 0.002296 Yes Difference: (Reach=R07)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.981160 0.000000 0.997129 Power (Alpha=.010) 0.768485 0.000000 0.898573 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 28.4689 3.9162 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.006753 0.083187 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R12 Count 5 5 Median 262 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:16 AM 221 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:16 AM 222 450 n Largemouth_Bass_PFOS_ppb vs Groups 50 Reach=R07 Reach=R12 Groups 5/1/2013 10:39:17 AM 223 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 286.2 87.50543 39.13362 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (R e ac h =R07) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 177.5477 60.96663 95.0% UCL of Mean 394.8523 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 209.18 62.54741 Unequal 4.17 209.18 88.4554 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7313 Standard Error 39.55846 39.55846 95.0% LCL Difference 117.958 101.1358 95.0% UCL Difference 300.4019 317.2242 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.2879 0.000739 Yes Difference < 0 5.2879 0.999630 No Difference > 0 5.2879 0.000370 Yes Difference: (Reach=R 07)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.995659 0.000000 0.999160 Power (Alpha=.010) 0.939867 0.000000 0.976954 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.2879 0.005443 Yes Difference < 0 5.2879 0.997278 No Difference > 0 5.2879 0.002722 Yes Difference: (Reach=R 07)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.974268 0.000000 0.995695 Power (Alpha=.010) 0.731162 0.000000 0.874901 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 45.8084 4.0605 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.002699 0.078657 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R13 Count 5 5 Median 262 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:17 AM 224 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:17 AM 225 450 n Largemouth_Bass_PFOS_ppb vs Groups 5 0 J -------------------------------------1-------------------------------------------------------------------------- T---------- Reach=R07 Reach=R13 Groups 5/1/2013 10:39:17 AM 226 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 286.2 87.50543 39.13362 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 177.5477 50.37198 95.0% UCL of Mean 394.8523 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 212.88 63.2407 Unequal 4.36 212.88 89.43585 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6892 Standard Error 39.99693 39.99693 95.0% LCL Difference 120.6469 105.3214 95.0% UCL Difference 305.1131 320.4386 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.3224 0.000709 Yes Difference < 0 5.3224 0.999646 No Difference > 0 5.3224 0.000354 Yes Difference: (Reach=R07)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996018 0.000000 0.999244 Power (Alpha=.010) 0.942876 0.000000 0.978399 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.3224 0.004702 Yes Difference < 0 5.3224 0.997649 No Difference > 0 5.3224 0.002351 Yes Difference: (Reach=R07)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.978780 0.000000 0.996528 Power (Alpha=.010) 0.760372 0.000000 0.892271 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 22.4175 3.2160 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.010630 0.110676 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R14 Count 5 5 Median 262 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:17 AM 227 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:17 AM 228 5/1/2013 10:39:17 AM 229 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 286.2 87.50543 39.13362 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 177.5477 66.08784 95.0% UCL of Mean 394.8523 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 201.26 62.80017 Unequal 4.24 201.26 88.81285 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7154 Standard Error 39.71832 39.71832 95.0% LCL Difference 109.6694 93.40875 95.0% UCL Difference 292.8506 309.1112 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.0672 0.000968 Yes Difference < 0 5.0672 0.999516 No Difference > 0 5.0672 0.000484 Yes Difference: (Reach=R 07)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.992601 0.000000 0.998383 Power (Alpha=.010) 0.917572 0.000000 0.965653 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.0672 0.006109 Yes Difference < 0 5.0672 0.996945 No Difference > 0 5.0672 0.003055 Yes Difference: (Reach=R 07)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.965307 0.000000 0.993303 Power (Alpha=.010) 0.700122 0.000000 0.851532 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 33.2166 3.6689 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.005025 0.091760 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R15 Count 5 5 Median 262 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:17 AM 230 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:17 AM 231 450 n Largemouth_Bass_PFOS_ppb vs Groups 5 0 J -------------------------------------1-------------------------------------------------------------------------- T---------- Reach=R07 Reach=R15 Groups 5/1/2013 10:39:18 AM 232 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 102.24 51.72672 23.13289 Reach=R09 5 231.8 57.10692 25.53899 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 38.0128 160.8924 95.0% UCL of Mean 166.4672 302.7076 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -129.56 54.48327 Unequal 7.92 -129.56 77.05098 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3099 Standard Error 34.45824 34.45824 95.0% LCL Difference -209.0209 -209.1556 95.0% UCL Difference -50.09915 -49.9644 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.7599 0.005545 Yes Difference < 0 -3.7599 0.002773 Yes Difference > 0 -3.7599 0.997227 No Difference: (Reach=R08)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.906963 0.961911 0.000000 Power (Alpha=.010) 0.655841 0.782038 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.7599 0.005643 Yes Difference < 0 -3.7599 0.002822 Yes Difference > 0 -3.7599 0.997178 No Difference: (Reach=R08)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.906165 0.961619 0.000000 Power (Alpha=.010) 0.653162 0.780182 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.2188 0.0657 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.852536 0.804115 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R09 Count 5 5 Median 80.3 259 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:18 AM 233 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 2 17 23 38 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.015873 Yes 0.984127 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.1934 0.028280 Yes -2.1934 0.014140 Yes -2.1934 0.985860 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.0889 0.036714 Yes -2.0889 0.018357 Yes -2.2978 0.989214 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:18 AM 234 5/1/2013 10:39:18 AM 235 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 102.24 51.72672 23.13289 Reach=R10 5 133.62 61.49823 27.50284 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 38.0128 57.25986 95.0% UCL of Mean 166.4672 209.9801 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -31.38 56.82291 Unequal 7.77 -31.38 80.35973 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3178 Standard Error 35.93796 35.93796 95.0% LCL Difference -114.2531 -114.6784 95.0% UCL Difference 51.49308 51.91843 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.8732 0.408014 No Difference < 0 -0.8732 0.204007 No Difference > 0 -0.8732 0.795993 No Difference: (Reach=R 08)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.120664 0.199262 0.007151 Power (Alpha=.010) 0.031595 0.055993 0.001059 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.8732 0.408737 No Difference < 0 -0.8732 0.204368 No Difference > 0 -0.8732 0.795632 No Difference: (Reach=R 08)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.120140 0.198683 0.007188 Power (Alpha=.010) 0.031311 0.055587 0.001070 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.4135 0.0974 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.745523 0.762999 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R10 Count 5 5 Median 80.3 104 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:18 AM 236 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 8 23 17 32 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.210318 No 0.789683 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.9400 0.347208 No -0.9400 0.173604 No -0.9400 0.826396 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.8356 0.403395 No -0.8356 0.201698 No -1.0445 0.851865 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:18 AM 237 5/1/2013 10:39:18 AM 238 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 102.24 51.72672 23.13289 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 38.0128 97.42249 95.0% UCL of Mean 166.4672 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -59.96 51.94879 Unequal 8.00 -59.96 73.46668 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3060 Standard Error 32.8553 32.8553 95.0% LCL Difference -135.7245 -135.7254 95.0% UCL Difference 15.80445 15.80541 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8250 0.105446 No Difference < 0 -1.8250 0.052723 No Difference > 0 -1.8250 0.947277 No Difference: (Reach=R08)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.362527 0.509452 0.000432 Power (Alpha=.010) 0.134904 0.212690 0.000048 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8250 0.105449 No Difference < 0 -1.8250 0.052724 No Difference > 0 -1.8250 0.947276 No Difference: (Reach=R08)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.362522 0.509448 0.000432 Power (Alpha=.010) 0.134899 0.212684 0.000048 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.0172 0.0225 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.987203 0.884454 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R11 Count 5 5 Median 80.3 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:18 AM 239 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 5 20 20 35 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.075397 No 0.924603 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.5667 0.117185 No -1.5667 0.058593 No -1.5667 0.941407 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.4623 0.143672 No -1.4623 0.071836 No -1.6711 0.952654 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:18 AM 240 5/1/2013 10:39:19 AM 241 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 102.24 51.72672 23.13289 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (R e ac h =R08) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 38.0128 49.75645 95.0% UCL of Mean 166.4672 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 32.12 38.37069 Unequal 4.80 32.12 54.26435 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6038 Standard Error 24.26776 24.26776 95.0% LCL Difference -23.84155 -31.06826 95.0% UCL Difference 88.08154 95.30826 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.3236 0.222218 No Difference < 0 1.3236 0.888891 No Difference > 0 1.3236 0.111109 No Difference: (Reach=R08)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.215289 0.002077 0.332534 Power (Alpha=.010) 0.066569 0.000268 0.112562 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.3236 0.245222 No Difference < 0 1.3236 0.877389 No Difference > 0 1.3236 0.122611 No Difference: (Reach=R08)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.188734 0.002485 0.306622 Power (Alpha=.010) 0.050847 0.000374 0.091165 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 9.9479 2.3668 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.047012 0.162503 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R12 Count 5 5 Median 80.3 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:19 AM 242 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 18 33 7 22 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.845238 No 0.154762 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.1489 0.250592 No 1.1489 0.874704 No 1.1489 0.125296 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.0445 0.296270 No 1.2534 0.894962 No 1.0445 0.148135 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.200000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:19 AM 243 5/1/2013 10:39:19 AM 244 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 102.24 51.72672 23.13289 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 38.0128 60.96663 95.0% UCL of Mean 166.4672 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 25.22 37.70153 Unequal 4.50 25.22 53.31801 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6593 Standard Error 23.84454 23.84454 95.0% LCL Difference -29.7656 -38.19092 95.0% UCL Difference 80.2056 88.63092 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.0577 0.321088 No Difference < 0 1.0577 0.839456 No Difference > 0 1.0577 0.160544 No Difference: (Reach=R 08)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.154597 0.004394 0.249642 Power (Alpha=.010) 0.043353 0.000614 0.075665 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.0577 0.343663 No Difference < 0 1.0577 0.828168 No Difference > 0 1.0577 0.171832 No Difference: (Reach=R 08)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.135611 0.005143 0.229214 Power (Alpha=.010) 0.033489 0.000826 0.061419 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 16.0068 2.5471 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.019931 0.149161 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R13 Count 5 5 Median 80.3 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:19 AM 245 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 17 32 8 23 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.789683 No 0.210318 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.9400 0.347208 No 0.9400 0.826396 No 0.9400 0.173604 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.8356 0.403395 No 1.0445 0.851865 No 0.8356 0.201698 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:19 AM 246 Largemouth_Bass_PFOS_ppb vs Groups 180 n 160 40 J-----------------------------1---------------------------------------------------------- T-------- Reach=R08 Reach=R13 Groups 5/1/2013 10:39:19 AM 247 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 102.24 51.72672 23.13289 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 38.0128 50.37198 95.0% UCL of Mean 166.4672 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 28.92 38.84086 Unequal 5.00 28.92 54.92927 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5698 Standard Error 24.56512 24.56512 95.0% LCL Difference -27.72726 -34.20807 95.0% UCL Difference 85.56726 92.04807 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1773 0.272920 No Difference < 0 1.1773 0.863540 No Difference > 0 1.1773 0.136460 No Difference: (Reach=R08)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.180210 0.003159 0.285559 Power (Alpha=.010) 0.052809 0.000426 0.090945 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1773 0.292018 No Difference < 0 1.1773 0.853991 No Difference > 0 1.1773 0.146009 No Difference: (Reach=R08)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.161452 0.003635 0.266430 Power (Alpha=.010) 0.042285 0.000555 0.076285 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 7.8333 1.6097 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.071092 0.240203 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R14 Count 5 5 Median 80.3 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:19 AM 248 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 16 31 9 24 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.726191 No 0.273810 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.7311 0.464702 No 0.7311 0.767649 No 0.7311 0.232351 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.6267 0.530869 No 0.8356 0.798302 No 0.6267 0.265435 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:19 AM 249 Largemouth_Bass_PFOS_ppb vs Groups 180 n 160 40 J----------------------R--e--a-c--h1-=--R--0--8--------------------------------------------R--e--a--c-h1-=--R--1-4-Groups 5/1/2013 10:39:19 AM 250 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 102.24 51.72672 23.13289 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 38.0128 66.08784 95.0% UCL of Mean 166.4672 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 17.3 38.11939 Unequal 4.68 17.3 53.90896 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6236 Standard Error 24.10882 24.10882 95.0% LCL Difference -38.29504 -45.95187 95.0% UCL Difference 72.89503 80.55186 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7176 0.493434 No Difference < 0 0.7176 0.753283 No Difference > 0 0.7176 0.246717 No Difference: (Reach=R 08)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.097351 0.010558 0.161916 Power (Alpha=.010) 0.024030 0.001644 0.042722 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7176 0.507192 No Difference < 0 0.7176 0.746404 No Difference > 0 0.7176 0.253596 No Difference: (Reach=R 08)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.089698 0.011569 0.152407 Power (Alpha=.010) 0.020478 0.001965 0.037133 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 11.6069 2.1059 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.035755 0.184789 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R15 Count 5 5 Median 80.3 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:20 AM 251 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 14 29 11 26 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.841270 No 0.579365 No 0.420635 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.3133 0.754023 No 0.3133 0.622989 No 0.3133 0.377011 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.2089 0.834532 No 0.4178 0.661948 No 0.2089 0.417266 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:20 AM 252 5/1/2013 10:39:20 AM 253 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 231.8 57.10692 25.53899 Reach=R10 5 133.62 61.49823 27.50284 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 160.8924 57.25986 95.0% UCL of Mean 302.7076 209.9801 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 98.18 59.3432 Unequal 7.96 98.18 83.92397 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3082 Standard Error 37.53194 37.53194 95.0% LCL Difference 11.6312 11.54875 95.0% UCL Difference 184.7288 184.8112 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.6159 0.030846 Yes Difference < 0 2.6159 0.984577 No Difference > 0 2.6159 0.015423 Yes Difference: (Reach=R 09)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.631554 0.000024 0.770727 Power (Alpha=.010) 0.314946 0.000002 0.441389 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.6159 0.030988 Yes Difference < 0 2.6159 0.984506 No Difference > 0 2.6159 0.015494 Yes Difference: (Reach=R 09)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.630894 0.000024 0.770336 Power (Alpha=.010) 0.314005 0.000002 0.440484 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.1597 0.0049 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.889280 0.945923 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R10 Count 5 5 Median 259 104 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:20 AM 254 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 21 36 4 19 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.952381 No 0.047619 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.7756 0.075800 No 1.7756 0.962100 No 1.7756 0.037900 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.6711 0.094693 No 1.8800 0.969949 No 1.6711 0.047346 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:20 AM 255 5/1/2013 10:39:20 AM 256 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 231.8 57.10692 25.53899 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 160.8924 97.42249 95.0% UCL of Mean 302.7076 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 69.6 54.69415 Unequal 7.94 69.6 77.34921 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3093 Standard Error 34.59162 34.59162 95.0% LCL Difference -10.16841 -10.28146 95.0% UCL Difference 149.3684 149.4815 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0120 0.079027 No Difference < 0 2.0120 0.960487 No Difference > 0 2.0120 0.039513 Yes Difference: (Reach=R09)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.425160 0.000228 0.576801 Power (Alpha=.010) 0.169626 0.000024 0.260189 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0120 0.079316 No Difference < 0 2.0120 0.960342 No Difference > 0 2.0120 0.039658 Yes Difference: (Reach=R09)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.424406 0.000229 0.576243 Power (Alpha=.010) 0.168894 0.000025 0.259373 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.1982 0.0184 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.865106 0.895341 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R11 Count 5 5 Median 259 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:20 AM 257 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 21 36 4 19 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.952381 No 0.047619 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.7756 0.075800 No 1.7756 0.962100 No 1.7756 0.037900 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.6711 0.094693 No 1.8800 0.969949 No 1.6711 0.047346 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:20 AM 258 5/1/2013 10:39:20 AM 259 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 231.8 57.10692 25.53899 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 160.8924 49.75645 95.0% UCL of Mean 302.7076 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 161.68 42.0129 Unequal 4.66 161.68 59.41521 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6289 Standard Error 26.57129 26.57129 95.0% LCL Difference 100.4065 91.82694 95.0% UCL Difference 222.9535 231.5331 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 6.0848 0.000294 Yes Difference < 0 6.0848 0.999853 No Difference > 0 6.0848 0.000147 Yes Difference: (Reach=R09)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999512 0.000000 0.999941 Power (Alpha=.010) 0.983987 0.000000 0.995598 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 6.0848 0.002222 Yes Difference < 0 6.0848 0.998889 No Difference > 0 6.0848 0.001111 Yes Difference: (Reach=R09)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996108 0.000000 0.999626 Power (Alpha=.010) 0.893998 0.000000 0.965456 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 12.1249 3.1469 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.033061 0.113997 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R12 Count 5 5 Median 259 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:20 AM 260 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:21 AM 261 5/1/2013 10:39:21 AM 262 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 231.8 57.10692 25.53899 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (R e ac h =R09) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 160.8924 60.96663 95.0% UCL of Mean 302.7076 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 154.78 41.40264 Unequal 4.41 154.78 58.55217 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6778 Standard Error 26.18533 26.18533 95.0% LCL Difference 94.39652 84.66182 95.0% UCL Difference 215.1635 224.8982 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.9109 0.000357 Yes Difference < 0 5.9109 0.999821 No Difference > 0 5.9109 0.000179 Yes Difference: (Reach=R 09)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999186 0.000000 0.999891 Power (Alpha=.010) 0.978093 0.000000 0.993499 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.9109 0.003001 Yes Difference < 0 5.9109 0.998499 No Difference > 0 5.9109 0.001501 Yes Difference: (Reach=R 09)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.993000 0.000000 0.999242 Power (Alpha=.010) 0.852664 0.000000 0.946759 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 19.5098 3.3493 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.013800 0.104618 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R13 Count 5 5 Median 259 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:21 AM 263 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:21 AM 264 5/1/2013 10:39:21 AM 265 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 231.8 57.10692 25.53899 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 160.8924 50.37198 95.0% UCL of Mean 302.7076 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 158.48 42.44274 Unequal 4.83 158.48 60.02309 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5982 Standard Error 26.84314 26.84314 95.0% LCL Difference 96.5796 88.7352 95.0% UCL Difference 220.3804 228.2248 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.9039 0.000360 Yes Difference < 0 5.9039 0.999820 No Difference > 0 5.9039 0.000180 Yes Difference: (Reach=R09)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999169 0.000000 0.999888 Power (Alpha=.010) 0.977820 0.000000 0.993398 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.9039 0.002232 Yes Difference < 0 5.9039 0.998884 No Difference > 0 5.9039 0.001116 Yes Difference: (Reach=R09)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.995120 0.000000 0.999460 Power (Alpha=.010) 0.887799 0.000000 0.961503 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 9.5476 2.3299 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.050523 0.165428 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R14 Count 5 5 Median 259 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:21 AM 266 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:21 AM 267 5/1/2013 10:39:21 AM 268 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 231.8 57.10692 25.53899 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 160.8924 66.08784 95.0% UCL of Mean 302.7076 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 146.86 41.78351 Unequal 4.56 146.86 59.0908 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6465 Standard Error 26.42621 26.42621 95.0% LCL Difference 85.92105 76.92341 95.0% UCL Difference 207.799 216.7966 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.5574 0.000536 Yes Difference < 0 5.5574 0.999732 No Difference > 0 5.5574 0.000268 Yes Difference: (Reach=R 09)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.997830 0.000000 0.999640 Power (Alpha=.010) 0.960292 0.000000 0.986327 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 5.5574 0.003452 Yes Difference < 0 5.5574 0.998274 No Difference > 0 5.5574 0.001726 Yes Difference: (Reach=R 09)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.988008 0.000000 0.998321 Power (Alpha=.010) 0.820417 0.000000 0.927456 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 14.1470 2.8791 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.025001 0.128173 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R15 Count 5 5 Median 259 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:21 AM 269 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:21 AM 270 5/1/2013 10:39:22 AM 271 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R10 5 133.62 61.49823 27.50284 Reach=R11 5 162.2 52.16991 23.33109 Note: T-alpha (Reach:=R10) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 57.25986 97.42249 95.0% UCL of Mean 209.9801 226.9775 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -28.58 57.02514 Unequal 7.79 -28.58 80.64572 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3167 Standard Error 36.06586 36.06586 95.0% LCL Difference -111.748 -112.1344 95.0% UCL Difference 54.58803 54.97439 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.7924 0.450962 No Difference < 0 -0.7924 0.225481 No Difference > 0 -0.7924 0.774519 No Difference: (Reach=R10)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.107965 0.179281 0.008774 Power (Alpha=.010) 0.027421 0.048754 0.001333 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.7924 0.451552 No Difference < 0 -0.7924 0.225776 No Difference > 0 -0.7924 0.774224 No Difference: (Reach=R10)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.107577 0.178837 0.008810 Power (Alpha=.010) 0.027218 0.048456 0.001345 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.3896 0.0413 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.757615 0.843992 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R11 Count 5 5 Median 104 145 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:22 AM 272 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 6 21 19 34 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.111111 No 0.888889 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.3578 0.174525 No -1.3578 0.087263 No -1.3578 0.912737 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.2534 0.210075 No -1.2534 0.105038 No -1.4623 0.928164 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:22 AM 273 240 n Largemouth_Bass_PFOS_ppb vs Groups 60J----------------1-------------------------------- T---- Reach=R10 Reach=R11 Groups 5/1/2013 10:39:22 AM 274 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R10 5 133.62 61.49823 27.50284 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (Reach:=R10) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 57.25986 49.75645 95.0% UCL of Mean 209.9801 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 63.5 45.00555 Unequal 4.57 63.5 63.64746 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6458 Standard Error 28.46401 28.46401 95.0% LCL Difference -2.138122 -11.8108 95.0% UCL Difference 129.1381 138.8108 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2309 0.056219 No Difference < 0 2.2309 0.971890 No Difference > 0 2.2309 0.028110 Yes Difference: (Reach=R10)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.500691 0.000104 0.652606 Power (Alpha=.010) 0.216719 0.000011 0.321774 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2309 0.081182 No Difference < 0 2.2309 0.959409 No Difference > 0 2.2309 0.040591 Yes Difference: (Reach=R10)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.423576 0.000153 0.596750 Power (Alpha=.010) 0.144852 0.000021 0.239379 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 14.0613 2.8324 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.025279 0.130878 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R12 Count 5 5 Median 104 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:22 AM 275 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 21 36 4 19 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.952381 No 0.047619 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.7756 0.075800 No 1.7756 0.962100 No 1.7756 0.037900 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.6711 0.094693 No 1.8800 0.969949 No 1.6711 0.047346 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:22 AM 276 220n Largemouth_Bass_PFOS_ppb vs Groups 40J----------------1-------------------------------- T---- Reach=R10 Reach=R12 Groups 5/1/2013 10:39:22 AM 277 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R10 5 133.62 61.49823 27.50284 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (R e ac h =R10) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 57.25986 60.96663 95.0% UCL of Mean 209.9801 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 56.6 44.43641 Unequal 4.35 56.6 62.84257 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6899 Standard Error 28.10405 28.10405 95.0% LCL Difference -8.208062 -18.99677 95.0% UCL Difference 121.4081 132.1968 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0139 0.078795 No Difference < 0 2.0139 0.960603 No Difference > 0 2.0139 0.039397 Yes Difference: (Reach=R 10)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.425807 0.000227 0.577475 Power (Alpha=.010) 0.170005 0.000024 0.260697 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0139 0.108567 No Difference < 0 2.0139 0.945716 No Difference > 0 2.0139 0.054284 No Difference: (Reach=R 10)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.352608 0.000328 0.519860 Power (Alpha=.010) 0.110682 0.000046 0.188996 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 22.6256 2.9891 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.010446 0.122087 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R13 Count 5 5 Median 104 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:22 AM 278 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 35 5 20 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.924603 No 0.075397 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.5667 0.117185 No 1.5667 0.941407 No 1.5667 0.058593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.4623 0.143672 No 1.6711 0.952654 No 1.4623 0.071836 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.200000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:22 AM 279 220n Largemouth_Bass_PFOS_ppb vs Groups 60J----------------1-------------------------------- T---- Reach=R10 Reach=R13 Groups 5/1/2013 10:39:23 AM 280 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R10 5 133.62 61.49823 27.50284 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R10) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 57.25986 50.37198 95.0% UCL of Mean 209.9801 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 60.3 45.40707 Unequal 4.72 60.3 64.21529 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6177 Standard Error 28.71795 28.71795 95.0% LCL Difference -5.923717 -14.87579 95.0% UCL Difference 126.5237 135.4758 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0997 0.068966 No Difference < 0 2.0997 0.965517 No Difference > 0 2.0997 0.034483 Yes Difference: (Reach=R10)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.455266 0.000167 0.607704 Power (Alpha=.010) 0.187671 0.000017 0.284149 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.0997 0.093128 No Difference < 0 2.0997 0.953436 No Difference > 0 2.0997 0.046564 Yes Difference: (Reach=R10)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.389560 0.000233 0.558067 Power (Alpha=.010) 0.130146 0.000031 0.216704 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 11.0724 2.1157 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.038895 0.183882 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R14 Count 5 5 Median 104 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:23 AM 281 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 22 37 3 18 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.972222 No 0.027778 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.9845 0.047202 Yes 1.9845 0.976399 No 1.9845 0.023601 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.8800 0.060103 No 2.0889 0.981643 No 1.8800 0.030051 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Normal Probability Plot of Largem outh_B ass_P FO S _ppb w hen Reach=R 10 5/1/2013 10:39:23 AM 282 1 5 10 25 50 75 90 95 99 Percent of Values 220n Largemouth_Bass_PFOS_ppb vs Groups 40 J-------------------- 1------------------------------------------1------ Reach=R10 Reach=R14 Groups 5/1/2013 10:39:23 AM 283 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R10 5 133.62 61.49823 27.50284 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R10) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 57.25986 66.08784 95.0% UCL of Mean 209.9801 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 48.68 44.79149 Unequal 4.49 48.68 63.34473 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6618 Standard Error 28.32862 28.32862 95.0% LCL Difference -16.64593 -26.72476 95.0% UCL Difference 114.0059 124.0848 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.7184 0.124046 No Difference < 0 1.7184 0.937977 No Difference > 0 1.7184 0.062023 No Difference: (Reach=R 10)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.328302 0.000614 0.470807 Power (Alpha=.010) 0.117426 0.000071 0.188011 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.7184 0.153047 No Difference < 0 1.7184 0.923476 No Difference > 0 1.7184 0.076524 No Difference: (Reach=R 10)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.276392 0.000817 0.425883 Power (Alpha=.010) 0.081009 0.000118 0.141589 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 16.4063 2.5801 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.019045 0.146882 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R15 Count 5 5 Median 104 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:23 AM 284 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 35 5 20 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.924603 No 0.075397 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.5667 0.117185 No 1.5667 0.941407 No 1.5667 0.058593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.4623 0.143672 No 1.6711 0.952654 No 1.4623 0.071836 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Normal ProbabilityPlotof Largernouth_Bass_PFGS_ppbwhenReach=R10 5/1/2013 10:39:23 AM 285 1 220n 5 10 25 50 75 90 95 Percent ofValues 99 Largemouth_Bass_PFOS_ppbvsGroups 60 J---------------------------------1------------------------------------------------------------------ J--------- Reach=R10 Reach=R15 Groups 5/1/2013 10:39:23 AM 286 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R11 5 162.2 52.16991 23.33109 Reach=R12 5 70.12 16.40021 7.334398 Note: T-alpha (Reach:=R11) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 97.42249 49.75645 95.0% UCL of Mean 226.9775 90.48355 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 92.08 38.66954 Unequal 4.78 92.08 54.68699 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6061 Standard Error 24.45677 24.45677 95.0% LCL Difference 35.6826 28.34396 95.0% UCL Difference 148.4774 155.816 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.7650 0.005505 Yes Difference < 0 3.7650 0.997248 No Difference > 0 3.7650 0.002752 Yes Difference: (Reach=R11)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.907689 0.000000 0.962289 Power (Alpha=.010) 0.657274 0.000000 0.783243 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.7650 0.014205 Yes Difference < 0 3.7650 0.992897 No Difference > 0 3.7650 0.007103 Yes Difference: (Reach=R11)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.841003 0.000000 0.937140 Power (Alpha=.010) 0.474847 0.000000 0.643114 Tests of Assumptions Section Assumption Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 10.1191 4.2298 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.045621 0.073744 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11 Reach=R12 Count 5 5 Median 145 65.8 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:23 AM 287 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:23 AM 288 Normal ProbabilityPlotof Largernouth_Bass_PFGS_ppbwhenReach=F,'11 Normal ProbabilityPlotof Largernouth_Bass_PFOS_ppbwhenReach=R12 Largemouth_Bass_PFOS_ppbvsGroups 240n 40 J---------------------------------1------------------------------------------------------------------ T--------- Reach=R11 Reach=R12 Groups 5/1/2013 10:39:24 AM 289 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R11 5 162.2 52.16991 23.33109 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (R e ac h =R11) = 2.7764, T-alpha (Reach=R13) = 2.7764 95.0% LCL of Mean 97.42249 60.96663 95.0% UCL of Mean 226.9775 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 85.18 38.00564 Unequal 4.49 85.18 53.74809 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6610 Standard Error 24.03688 24.03688 95.0% LCL Difference 29.75086 21.2169 95.0% UCL Difference 140.6091 149.1431 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.5437 0.007579 Yes Difference < 0 3.5437 0.996211 No Difference > 0 3.5437 0.003789 Yes Difference: (Reach=R11)-(Reach=R13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.872162 0.000000 0.942807 Power (Alpha=.010) 0.593155 0.000000 0.727458 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.5437 0.019751 Yes Difference < 0 3.5437 0.990124 No Difference > 0 3.5437 0.009876 Yes Difference: (Reach=R11)-(Reach=R13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.783443 0.000001 0.905277 Power (Alpha=.010) 0.396420 0.000000 0.563574 Tests of Assumptions Section Assumption Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 16.2823 4.6207 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.019314 0.063818 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11 Reach=R13 Count 5 5 Median 145 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:24 AM 290 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:24 AM 291 Largemouth_Bass_PFOS_ppbvsGroups 240n 60 J---------------------------------1------------------------------------------------------------------ T--------- Reach=R11 Reach=R13 Groups 5/1/2013 10:39:24 AM 292 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R11 5 162.2 52.16991 23.33109 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R11) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 97.42249 50.37198 95.0% UCL of Mean 226.9775 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 88.88 39.13612 Unequal 4.99 88.88 55.34683 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5724 Standard Error 24.75186 24.75186 95.0% LCL Difference 31.80212 25.20891 95.0% UCL Difference 145.9579 152.5511 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.5908 0.007076 Yes Difference < 0 3.5908 0.996462 No Difference > 0 3.5908 0.003538 Yes Difference: (Reach=R11)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.880433 0.000000 0.947512 Power (Alpha=.010) 0.607108 0.000000 0.739920 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.5908 0.015756 Yes Difference < 0 3.5908 0.992122 No Difference > 0 3.5908 0.007878 Yes Difference: (Reach=R11)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.815624 0.000001 0.921086 Power (Alpha=.010) 0.449020 0.000000 0.613498 Tests of Assumptions Section Assumption Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 7.9682 3.1333 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.069055 0.114668 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11 Reach=R14 Count 5 5 Median 145 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:24 AM 293 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:24 AM 294 5/1/2013 10:39:24 AM 295 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R11 5 162.2 52.16991 23.33109 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R11) = 2.7764, T-alpha (Reach=R15) = 2.7764 95.0% LCL of Mean 97.42249 66.08784 95.0% UCL of Mean 226.9775 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 77.26 38.4202 Unequal 4.67 77.26 54.33436 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6257 Standard Error 24.29907 24.29907 95.0% LCL Difference 21.22625 13.45834 95.0% UCL Difference 133.2937 141.0617 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.1795 0.013006 Yes Difference < 0 3.1795 0.993497 No Difference > 0 3.1795 0.006503 Yes Difference: (Reach=R11)-(Reach=R15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.794801 0.000002 0.894454 Power (Alpha=.010) 0.482246 0.000000 0.622074 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.1795 0.026983 Yes Difference < 0 3.1795 0.986509 No Difference > 0 3.1795 0.013491 Yes Difference: (Reach=R11)-(Reach=R15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.705802 0.000004 0.850344 Power (Alpha=.010) 0.327914 0.000000 0.481551 Tests of Assumptions Section Assumption Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 11.8066 3.9752 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.034679 0.081295 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11 Reach=R15 Count 5 5 Median 145 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:24 AM 296 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:24 AM 297 Largemouth_Bass_PFOS_ppbvsGroups Groups 5/1/2013 10:39:25 AM 298 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R12 5 70.12 16.40021 7.334398 Reach=R13 5 77.02 12.92892 5.781989 Note: T-alpha (Reach:=R12) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 49.75645 60.96663 95.0% UCL of Mean 90.48355 93.07338 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.9 14.76692 Unequal 7.59 -6.9 20.88358 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3281 Standard Error 9.339422 9.339422 95.0% LCL Difference -28.43674 -28.64276 95.0% UCL Difference 14.63675 14.84276 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.7388 0.481137 No Difference < 0 -0.7388 0.240569 No Difference > 0 -0.7388 0.759431 No Difference: (Reach=R 12)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.100247 0.166725 0.010023 Power (Alpha=.010) 0.024946 0.044368 0.001550 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.7388 0.482254 No Difference < 0 -0.7388 0.241127 No Difference > 0 -0.7388 0.758873 No Difference: (Reach=R 12)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.099564 0.165919 0.010101 Power (Alpha=.010) 0.024597 0.043846 0.001575 Tests of Assumptions Section Assumption Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.6091 0.0033 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.656197 0.955387 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R12 Reach=R13 Count 5 5 Median 65.8 71 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:25 AM 299 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R12 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 4 19 21 36 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.047619 Yes 0.952381 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.7756 0.075800 No -1.7756 0.037900 Yes -1.7756 0.962100 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.6711 0.094693 No -1.6711 0.047346 Yes -1.8800 0.969949 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:25 AM 300 Largemouth_Bass_PFOS_ppbvsGroups 100n 90 50 J---------------------------------1------------------------------------------------------------------ T--------- Reach=R12 Reach=R13 Groups 5/1/2013 10:39:25 AM 301 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R12 5 70.12 16.40021 7.334398 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R12) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 49.75645 50.37198 95.0% UCL of Mean 90.48355 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -3.2 17.47196 Unequal 7.89 -3.2 24.70909 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3117 Standard Error 11.05024 11.05024 95.0% LCL Difference -28.6819 -28.74476 95.0% UCL Difference 22.2819 22.34476 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.2896 0.779496 No Difference < 0 -0.2896 0.389748 No Difference > 0 -0.2896 0.610252 No Difference: (Reach=R12)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.057577 0.083966 0.027984 Power (Alpha=.010) 0.012121 0.018725 0.005042 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.2896 0.779598 No Difference < 0 -0.2896 0.389799 No Difference > 0 -0.2896 0.610201 No Difference: (Reach=R12)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.057552 0.083917 0.028004 Power (Alpha=.010) 0.012109 0.018700 0.005049 Tests of Assumptions Section Assumption Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.2699 0.6271 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.822462 0.451273 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R12 Reach=R14 Count 5 5 Median 65.8 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:25 AM 302 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R12 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 13 28 12 27 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 1.000000 No 0.579365 No 0.420635 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.1044 0.916815 No 0.1044 0.541593 No 0.1044 0.458407 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.0000 1.000000 No 0.2089 0.582734 No 0.0000 0.500000 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:25 AM 303 Largemouth_Bass_PFOS_ppbvsGroups 100 50 J---------------------------------1-------------------------------------------------------------------1--------- Reach=R12 Reach=R14 Groups 5/1/2013 10:39:25 AM 304 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R12 5 70.12 16.40021 7.334398 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R12) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 49.75645 66.08784 95.0% UCL of Mean 90.48355 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -14.82 15.80332 Unequal 7.95 -14.82 22.34927 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3084 Standard Error 9.994899 9.994899 95.0% LCL Difference -37.86828 -37.89206 95.0% UCL Difference 8.228278 8.252065 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.4828 0.176425 No Difference < 0 -1.4828 0.088213 No Difference > 0 -1.4828 0.911787 No Difference: (Reach=R 12)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.257875 0.386747 0.001290 Power (Alpha=.010) 0.084542 0.139931 0.000159 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.4828 0.176645 No Difference < 0 -1.4828 0.088323 No Difference > 0 -1.4828 0.911677 No Difference: (Reach=R 12)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.257555 0.386464 0.001292 Power (Alpha=.010) 0.084311 0.139642 0.000159 Tests of Assumptions Section Assumption Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.1668 0.1279 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.884778 0.729893 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R12 Reach=R15 Count 5 5 Median 65.8 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:25 AM 305 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R12 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 7 22 18 33 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.154762 No 0.845238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.1489 0.250592 No -1.1489 0.125296 No -1.1489 0.874704 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.0445 0.296270 No -1.0445 0.148135 No -1.2534 0.894962 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:25 AM 306 Largemouth_Bass_PFOS_ppb vs Groups 105 50 J---------------------------------1------------------------------------------------------------------ T--------- Reach=R12 Reach=R15 Groups 5/1/2013 10:39:25 AM 307 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R13 5 77.02 12.92892 5.781989 Reach=R14 5 73.32 18.48167 8.265252 Note: T-alpha (Reach:=R13) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 60.96663 50.37198 95.0% UCL of Mean 93.07338 96.26802 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 3.7 15.94881 Unequal 7.16 3.7 22.55502 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3540 Standard Error 10.08691 10.08691 95.0% LCL Difference -19.56046 -20.04507 95.0% UCL Difference 26.96046 27.44507 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.3668 0.723272 No Difference < 0 0.3668 0.638364 No Difference > 0 0.3668 0.361636 No Difference: (Reach=R13)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.062186 0.023716 0.095432 Power (Alpha=.010) 0.013435 0.004159 0.021925 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.3668 0.724362 No Difference < 0 0.3668 0.637819 No Difference > 0 0.3668 0.362181 No Difference: (Reach=R13)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.061842 0.023895 0.094864 Power (Alpha=.010) 0.013278 0.004225 0.021625 Tests of Assumptions Section Assumption Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.0434 0.9850 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.505882 0.350036 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13 Reach=R14 Count 5 5 Median 71 75.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:26 AM 308 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 14 29 11 26 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.841270 No 0.579365 No 0.420635 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.3133 0.754023 No 0.3133 0.622989 No 0.3133 0.377011 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.2089 0.834532 No 0.4178 0.661948 No 0.2089 0.417266 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:26 AM 309 Largemouth_Bass_PFOS_ppb vs Groups 100 50 J---------------------------------1-------------------------------------------------------------------1--------- Reach=R13 Reach=R14 Groups 5/1/2013 10:39:26 AM 310 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R13 5 77.02 12.92892 5.781989 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R13) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 60.96663 66.08784 95.0% UCL of Mean 93.07338 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -7.92 14.10106 Unequal 7.80 -7.92 19.94192 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3162 Standard Error 8.918296 8.918296 95.0% LCL Difference -28.48563 -28.57687 95.0% UCL Difference 12.64563 12.73687 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.8881 0.400419 No Difference < 0 -0.8881 0.200210 No Difference > 0 -0.8881 0.799790 No Difference: (Reach=R 13)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.123150 0.203088 0.006882 Power (Alpha=.010) 0.032426 0.057415 0.001014 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.8881 0.401056 No Difference < 0 -0.8881 0.200528 No Difference > 0 -0.8881 0.799472 No Difference: (Reach=R 13)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.122679 0.202571 0.006913 Power (Alpha=.010) 0.032170 0.057050 0.001024 Tests of Assumptions Section Assumption Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.3791 0.2408 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.763014 0.636803 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13 Reach=R15 Count 5 5 Median 71 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:26 AM 311 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 9 24 16 31 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.273810 No 0.726191 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.7311 0.464702 No -0.7311 0.232351 No -0.7311 0.767649 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.6267 0.530869 No -0.6267 0.265435 No -0.8356 0.798302 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:26 AM 312 5/1/2013 10:39:26 AM 313 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R14 5 73.32 18.48167 8.265252 Reach=R15 5 84.94 15.18298 6.790037 Note: T-alpha (Reach:=R14) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 50.37198 66.08784 95.0% UCL of Mean 96.26802 103.7922 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -11.62 16.91294 Unequal 7.71 -11.62 23.91851 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3212 Standard Error 10.69668 10.69668 95.0% LCL Difference -36.28659 -36.44931 95.0% UCL Difference 13.04659 13.20931 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.0863 0.308983 No Difference < 0 -1.0863 0.154492 No Difference > 0 -1.0863 0.845508 No Difference: (Reach=R 14)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.160473 0.258020 0.004065 Power (Alpha=.010) 0.045479 0.079134 0.000563 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.0863 0.310133 No Difference < 0 -1.0863 0.155066 No Difference > 0 -1.0863 0.844934 No Difference: (Reach=R 14)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.159404 0.256936 0.004099 Power (Alpha=.010) 0.044847 0.078274 0.000573 Tests of Assumptions Section Assumption Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.4817 0.3528 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.712493 0.568947 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R14 Reach=R15 Count 5 5 Median 75.3 86.9 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:39:26 AM 314 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R14 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 7 22 18 33 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.154762 No 0.845238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.1489 0.250592 No -1.1489 0.125296 No -1.1489 0.874704 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.0445 0.296270 No -1.0445 0.148135 No -1.2534 0.894962 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\bass_Nov_2012.NCSS Largemouth_Bass_PFOS_ppb 5/1/2013 10:39:26 AM 315 APPENDIX F-3 CHANNEL CATFISH STATISTICS TEMPORAL EVALUATION 4/10/2013 11:14:46 AM 1 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 01, D ate=Jul-07 6 2 .4 5 1 .2 1 6 1 4 1 0 .4 9 6 4 8 7 7 1 .1 7 3 7 3 8 3 .7 2 6 2 6 2 R e a c h = R 0 1 , D ate=Jul-08 6 1 .3 2 1 6 6 7 0 .7 9 9 1 3 5 0 .3 2 6 2 4 5 5 0 .4 8 3 0 2 6 2 .1 6 0 3 0 7 Note: T-alp h a (R each = R 01, D ate=Jul-07) = 2 .5 7 0 6 , T-alpha (R each = R 01, D ate=Ju l-08) = 2 .5 7 0 6 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 1.128333 1 .0 2 8 9 8 4 Unequal 8 .6 4 1 .1 2 8 3 3 3 1 .4 5 5 2 0 3 Note: T-alp h a (Equal) = 2 .2 2 8 1 , T-alpha (U nequal) = 2 .2 7 6 6 Standard Error 0 .5 9 4 0 8 4 3 0 .5 9 4 0 8 4 3 95.0% LCL Difference -0 .1 9 5 3 6 8 9 -0 .2 2 4 1 7 9 95.0% UCL Difference 2.45 2 0 3 6 2.48 0 8 4 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .8 9 9 3 0 .0 8 6 7 2 5 No D ifference < 0 1 .8 9 9 3 0 .9 5 6 6 3 7 No D ifference > 0 1 .8 9 9 3 0 .0 4 3 3 6 3 Yes Difference: (R e a c h = R 0 1 , D ate= Ju l-07)-(R each = R 01, D ate=Ju l-08) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 0 4 2 6 2 0 .0 0 0 3 0 6 0 .5 4 9 3 8 1 Power (Alpha=.010) 0 .1 6 4 4 6 8 0 .0 0 0 0 3 1 0 .2 4 9 2 5 9 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .8 9 9 3 0 .0 9 1 3 5 4 No D ifference < 0 1 .8 9 9 3 0 .9 5 4 3 2 3 No D ifference > 0 1 .8 9 9 3 0 .0 4 5 6 7 7 Yes Difference: (R e a c h = R 0 1 , D ate = J u l-0 7 )-(R e a c h = R 0 1 , D ate=Ju l-08) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .3 9 3 3 8 6 0 .0 0 0 3 2 5 0 .5 4 1 1 7 9 Power (Alpha=.010) 0 .1 5 3 9 2 5 0 .0 0 0 0 3 5 0 .2 3 7 5 6 4 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-08) Variance-R atio E qual-V ariance Test 2 .3 1 5 9 M odified-Levene E qual-V ariance Test 0 .2 6 6 0 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .3 7 8 0 5 6 0 .6 1 7 2 4 0 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-07 Reach=R01, Date=Jul-08 Count 6 6 Median 1.9 1.095 95.0% LCL of Median 1.5 0 .5 5 95.0% UCL of Median 4 .4 2 .4 8 4/10/2013 11:14:46 AM 2 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 01, D ate=Jul-07 R e a c h = R 0 1 , D ate=Jul-08 N u m b e r S ets o f T ie s = 1, Mann W Whitney U Sum Ranks 29 50 7 28 Multiplicity F acto r = 6 Mean of W 39 39 Std Dev of W 6 .2 3 4 0 7 1 6 .2 3 4 0 7 1 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1 .7 6 4 5 0.077648 No 1 .7 6 4 5 0.961176 No 1 .7 6 4 5 0.038824 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1 .6 8 4 3 0.092125 No 1 .8 4 4 7 0.967460 No 1 .6 8 4 3 0.046063 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 6 6 6 6 7 0 .0 0 0 0 0 0 0 .6 6 6 6 6 7 Reject H0 if Greater Than 0 .6 9 8 0 0 .6 9 8 0 0 .6 9 8 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .1 4 2 9 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFGS_ppb when Reach=R01, Date=Jul 4/10/2013 11:14:46 AM 3 0.5 J--------------------------1---------------------------------------------------- 1--------------- Reach=R01,Date=Jul-07 Reach=R01,Date=Jul-Q8 Groups 4/10/2013 11:14:46 AM 4 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 01, D ate=Jul-07 6 2 .4 5 1 .2 1 6 1 4 1 0 .4 9 6 4 8 7 7 1 .1 7 3 7 3 8 3 .7 2 6 2 6 2 R e a c h = R 0 1 , D ate=Jul-09 6 1.87 1 .1 1 7 8 9 1 0 .4 5 6 3 7 7 0 .6 9 6 8 4 5 5 3 .0 4 3 1 5 4 Note: T-alp h a (R each = R 01, D ate=Jul-07) = 2 .5 7 0 6 , T-alpha (R each = R 01, D ate=Ju l-09) = 2 .5 7 0 6 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 0.58 1 .1 6 8 0 5 Unequal 9.93 0.58 1 .6 5 1 8 7 2 Note: T-alpha (Equal) = 2 .2 2 8 1 , T-alpha (U nequal) = 2 .2 3 0 3 Standard Error 0 .6 7 4 3 7 3 8 0 .6 7 4 3 7 3 8 95.0% LCL Difference -0 .9 2 2 5 9 8 4 -0 .9 2 4 0 3 8 2 95.0% UCL Difference 2.08 2 5 9 8 2 .0 8 4 0 3 8 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .8 6 0 1 0 .4 0 9 9 0 3 No D ifference < 0 0 .8 6 0 1 0 .7 9 5 0 4 9 No D ifference > 0 0 .8 6 0 1 0 .2 0 4 9 5 1 No Difference: (R e a c h = R 0 1 , D ate= Ju l-07)-(R each = R 01, D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 2 2 0 7 7 0 .0 0 7 1 4 1 0 .1 9 9 8 2 9 Power (Alpha=.010) 0 .0 3 2 8 6 5 0 .0 0 1 0 2 2 0 .0 5 7 5 6 9 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .8 6 0 1 0 .4 1 0 0 4 2 No D ifference < 0 0 .8 6 0 1 0 .7 9 4 9 7 9 No D ifference > 0 0 .8 6 0 1 0 .2 0 5 0 2 1 No Difference: (R e a c h = R 0 1 , D ate = J u l-0 7 )-(R e a c h = R 0 1 , D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 2 1 9 7 4 0 .0 0 7 1 4 8 0 .1 9 9 7 1 7 Power (Alpha=.010) 0 .0 3 2 8 0 6 0 .0 0 1 0 2 5 0 .0 5 7 4 8 7 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-09) Variance-R atio E qual-V ariance Test 1 .1 8 3 5 M odified-Levene E qual-V ariance Test 0.0191 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .8 5 7 8 3 1 0 .8 9 2 8 2 8 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-07 Reach=R01, Date=Jul-09 Count 6 6 Median 1.9 1.485 95.0% LCL of Median 1.5 0 .9 95.0% UCL of Median 4 .4 3 .7 7 4/10/2013 11:14:46 AM 5 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 01, D ate=Jul-07 R e a c h = R 0 1 , D ate=Jul-09 N um ber S ets of Ties = 1, Mann W Whitney U Sum Ranks 25 46 11 3 2 Multiplicity F acto r = 6 Mean of W 39 39 Std Dev of W 6 .2 3 4 0 7 1 6 .2 3 4 0 7 1 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1 .1 2 2 9 0.261496 No 1 .1 2 2 9 0.869252 No 1 .1 2 2 9 0.130748 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1 .0 4 2 7 0.297107 No 1.2031 0.885525 No 1 .0 4 2 7 0.148553 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .5 0 0 0 0 0 0 .0 0 0 0 0 0 0 .5 0 0 0 0 0 Reject H0 if Greater Than 0 .6 9 8 0 0 .6 9 8 0 0 .6 9 8 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .4 7 4 0 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFGS_ppb when Reach=R01, Date=Jul 4/10/2013 11:14:46 AM 6 0.5 J--------------------------1---------------------------------------------------- 1--------------- Reach=R01,Date=Jul-07 Reach=R01,Date=Jul-Q9 Groups 4/10/2013 11:14:46 AM 7 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 01, D ate=Jul-07 6 2 .4 5 1 .2 1 6 1 4 1 0 .4 9 6 4 8 7 7 1 .1 7 3 7 3 8 3 .7 2 6 2 6 2 R each=R 01, D ate=N ov-12 5 2 .0 1 6 8 1 .8 1 7 0 2 4 0 .8 1 2 5 9 7 9 -0 .2 3 9 3 3 3 6 4 .2 7 2 9 3 3 Note: T-alp h a (R each = R 01, D ate=Jul-07) = 2 .5 7 0 6 , T-alpha (R each = R 01, D ate=N o v-12) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 0.4332 1 .5 1 2 9 5 6 Unequal 6.79 0.4332 2 .1 8 6 4 5 3 Note: T-alpha (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .3 7 9 7 Standard Error 0 .9 1 6 1 4 0 .9 5 2 2 6 8 6 95.0% LCL Difference -1 .6 3 9 2 5 3 -1 .8 3 2 9 4 8 95.0% UCL Difference 2.50 5 6 5 3 2 .6 9 9 3 4 8 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .4 7 2 9 0 .6 4 7 5 7 5 No D ifference < 0 0 .4 7 2 9 0 .6 7 6 2 1 3 No D ifference > 0 0 .4 7 2 9 0 .3 2 3 7 8 7 No Difference: (R e a c h = R 0 1 , D ate= Ju l-07)-(R each = R 01, D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 7 0 8 9 3 0 .0 1 8 5 7 7 0 .1 1 3 7 9 0 Power (Alpha=.010) 0 .0 1 6 0 7 0 0 .0 0 3 1 0 6 0 .0 2 7 5 1 0 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .4 5 4 9 0 .6 6 3 3 6 4 No D ifference < 0 0 .4 5 4 9 0 .6 6 8 3 1 8 No D ifference > 0 0 .4 5 4 9 0 .3 3 1 6 8 2 No Difference: (R e a c h = R 01 , D ate = J u l-0 7 )-(R e a c h = R 0 1 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 6 7 9 9 8 0 .0 1 9 8 1 2 0 .1 0 8 6 7 8 Power (Alpha=.010) 0 .0 1 4 9 8 1 0 .0 0 3 4 2 3 0 .0 2 5 4 7 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 01 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 01 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 2 .2 3 2 3 M odified-Levene E qual-V ariance Test 0 .0 6 8 0 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .4 0 1 8 3 4 0 .8 0 0 1 5 3 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-07 Reach=R01, Date=Nov-12 Count 6 5 Median 1.9 1.5 95.0% LCL of Median 1.5 95.0% UCL of Median 4 .4 4/10/2013 11:14:47 AM 8 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 01, D ate=Jul-07 R each=R 01, D ate=N ov-12 N um ber S ets of Ties = 1, Mann W Whitney U Sum Ranks 20 41 10 25 Multiplicity F acto r = 24 Mean of W 36 30 Std Dev of W 5 .4 2 7 2 0 4 5 .4 2 7 2 0 4 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0 .9 2 1 3 0.356902 No -0 .9 2 1 3 0.821549 No -0 .9 2 1 3 0.178451 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0 .8 2 9 2 0.407016 No -1 .0 1 3 4 0.844569 No -0 .8 2 9 2 0.203508 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .2 0 0 0 0 0 0 .4 0 0 0 0 0 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .6 8 8 3 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFGS_ppb when Reach=R01, Date=Jul 4/10/2013 11:14:47 AM 9 4/10/2013 11:15:00 AM 109 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 01, D ate=Jul-08 6 1 .3 2 1 6 6 7 0 .7 9 9 1 3 5 0 .3 2 6 2 4 5 5 0 .4 8 3 0 2 6 2 .1 6 0 3 0 7 R e a c h = R 0 1 , D ate=Jul-09 6 1 .8 7 1 .1 1 7 8 9 1 0 .4 5 6 3 7 7 0 .6 9 6 8 4 5 5 3 .0 4 3 1 5 4 Note: T-alp h a (R each = R 01, D ate=Jul-08) = 2 .5 7 0 6 , T-alpha (R each = R 01, D ate=Ju l-09) = 2 .5 7 0 6 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -0 .5 4 8 3 3 3 3 0 .9 7 1 6 7 3 Unequal 9.05 -0 .5 4 8 3 3 3 3 1 .3 7 4 1 5 3 Note: T-alp h a (Equal) = 2 .2 2 8 1 , T-alpha (U nequal) = 2 .2 6 0 2 Standard Error 0 .5 6 0 9 9 5 6 0 .5 6 0 9 9 5 6 95.0% LCL Difference -1.79831 -1 .8 1 6 2 8 2 95.0% UCL Difference 0 .7 0 1 6 4 2 9 0 .7 1 9 6 1 5 2 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .9 7 7 4 0 .3 5 1 4 1 0 No D ifference < 0 -0 .9 7 7 4 0 .1 7 5 7 0 5 No D ifference > 0 -0 .9 7 7 4 0 .8 2 4 2 9 5 No Difference: (R e a c h = R 0 1 , D ate= Ju l-08)-(R each = R 01, D ate=Ju l-09) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 4 3 6 7 0 0 .2 3 1 8 0 9 0.005231 Power (Alpha=.010) 0 .0 4 0 5 7 8 0 .0 7 0 3 0 2 0 .0 0 0 7 1 8 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .9 7 7 4 0 .3 5 3 7 5 4 No D ifference < 0 -0 .9 7 7 4 0 .1 7 6 8 7 7 No D ifference > 0 -0 .9 7 7 4 0 .8 2 3 1 2 3 No Difference: (R e a c h = R 0 1 , D ate = J u l-0 8 )-(R e a c h = R 0 1 , D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 4 1 6 8 6 0 .2 2 9 7 5 8 0 .0 0 5 3 2 2 Power (Alpha=.010) 0 .0 3 9 3 9 2 0 .0 6 8 6 9 2 0 .0 0 0 7 4 4 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-08) Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-09) Variance-R atio E qual-V ariance Test 1 .9 5 6 9 M odified-Levene E qual-V ariance Test 0 .1 6 4 3 Probability 0.0000 Decision(.050) 1.000000 C annot reject norm ality 0.0000 1.000000 C annot reject norm ality 0 .4 7 8 9 3 8 0 .6 9 3 7 3 3 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-08 Reach=R01, Date=Jul-09 Count 6 6 Median 1.095 1.485 95.0% LCL of Median 0 .5 5 0.9 95.0% UCL of Median 2 .4 8 3 .7 7 4/10/2013 11:15:00 AM 110 Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 01, D ate=Jul-08 R e a c h = R 0 1 , D ate=Jul-09 N um ber Sets of T ies = 0, Mann W Whitney U Sum Ranks 11 3 2 25 46 Multiplicity F acto r = 0 Mean of W 39 39 Std Dev of W 6 .2 4 4 9 9 8 6.24 4 9 9 8 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.154762 No 0.845238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1 .1 2 0 9 0.262332 No -1 .1 2 0 9 0.131166 No -1 .1 2 0 9 0.868834 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1 .0 4 0 8 0.297953 No -1 .0 4 0 8 0.148977 No -1 .2 0 1 0 0.885117 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .5 0 0 0 0 0 0 .5 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 9 8 0 0 .6 9 8 0 0 .6 9 8 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .4 7 4 0 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:15:01 AM 111 Normal ProbabilityPlot ofChannel_Catfish_PFGS_ppbwhenReach=R01,Date=Jul- Normal ProbabilityPlot ofChannel_Catfish_PFOS_ppbwhenReach=R01, Date=Jul Channel_Catfish_F'FOS_ppbvs Groups 4.0n 3.53.0- 0.5J-------R-e-ac-h-=-R-0-1,1-D-a-te-=-J-ul--0-8-------------R-e-a-c-h-=-R-0-1,1-D-a-te-=-Ju-l--0-9 Groups 4/10/2013 11:15:01 AM 112 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 01, D ate=Jul-08 6 1 .3 2 1 6 6 7 0 .7 9 9 1 3 5 0 .3 2 6 2 4 5 5 0 .4 8 3 0 2 6 2 .1 6 0 3 0 7 R each=R 01, D ate=N ov-12 5 2 .0 1 6 8 1 .8 1 7 0 2 4 0 .8 1 2 5 9 7 9 -0 .2 3 9 3 3 3 6 4 .2 7 2 9 3 3 Note: T-alp h a (R each = R 01, D ate=Jul-08) = 2 .5 7 0 6 , T-alpha (R each = R 01, D ate=N o v-12) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 -0 .6 9 5 1 3 3 3 1 .3 4 9 8 7 2 Unequal 5.28 -0 .6 9 5 1 3 3 3 1 .9 8 4 9 9 2 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .5 2 9 6 Standard Error 0 .8 1 7 3 8 8 2 0 .8 7 5 6 4 3 5 95.0% LCL Difference -2 .5 4 4 1 9 4 -2 .9 1 0 1 8 5 95.0% UCL Difference 1 .1 5 3 9 2 7 1 .5 1 9 9 1 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .8 5 0 4 0 .4 1 7 1 3 5 No D ifference < 0 -0 .8 5 0 4 0 .2 0 8 5 6 7 No D ifference > 0 -0 .8 5 0 4 0 .7 9 1 4 3 3 No Difference: (R e a c h = R 0 1 , D ate= Ju l-08)-(R each = R 01, D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 1 8 8 6 8 0 .1 9 5 6 1 7 0 .0 0 7 4 3 6 Power (Alpha=.010) 0 .0 3 1 4 0 9 0 .0 5 5 3 6 7 0 .0 0 1 0 8 7 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .7 9 3 9 0 .4 6 1 4 3 1 No D ifference < 0 -0 .7 9 3 9 0 .2 3 0 7 1 5 No D ifference > 0 -0 .7 9 3 9 0 .7 6 9 2 8 5 No Difference: (R e a c h = R 01 , D ate = J u l-0 8 )-(R e a c h = R 0 1 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 0 1 1 1 9 0 .1 7 1 2 9 2 0 .0 0 9 4 3 4 Power (Alpha=.010) 0 .0 2 4 0 4 4 0.043651 0 .0 0 1 5 4 2 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-08) Skew ness Norm ality (R e a c h = R 01 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 01 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 5 .1 6 9 9 M odified-Levene E qual-V ariance Test 0 .4 2 9 8 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .1 0 0 8 1 4 0 .5 2 8 4 8 9 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-08 Reach=R01, Date=Nov-12 Count 6 5 Median 1.095 1.5 95.0% LCL of Median 0 .5 5 95.0% UCL of Median 2 .4 8 4/10/2013 11:15:01 AM 113 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 01, D ate=Jul-08 R each=R 01, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 10 31 20 35 Multiplicity F acto r = 0 Mean of W 36 30 Std Dev of W 5 .4 7 7 2 2 6 5 .4 7 7 2 2 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.428571 No 0.214286 No 0.785714 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0 .9 1 2 9 0.361310 No 0 .9 1 2 9 0.180655 No 0 .9 1 2 9 0.819345 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0 .8 2 1 6 0.411314 No 0 .8 2 1 6 0.205657 No 1 .0 0 4 2 0.842349 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .5 0 0 0 0 0 0 .5 0 0 0 0 0 0 .1 3 3 3 3 3 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal ProbabilityPlot ofChannel_Catfish_PFGS_ppbwhenReach=R01,Date=Jul- 4/10/2013 11:15:01 AM 114 Channel_Catfish_F'FOS_ppbvs Groups 5.5-| 4/10/2013 11:15:15 AM 214 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R e a c h = R 0 1 , D ate=Jul-09 6 1.87 1 .1 1 7 8 9 1 0 .4 5 6 3 7 7 0 .6 9 6 8 4 5 5 3 .0 4 3 1 5 4 R each=R 01, D ate=N ov-12 5 2 .0 1 6 8 1 .8 1 7 0 2 4 0 .8 1 2 5 9 7 9 -0 .2 3 9 3 3 3 6 4 .2 7 2 9 3 3 Note: T-alp h a (R each = R 01, D ate=Jul-09) = 2 .5 7 0 6 , T-alpha (R e a c h = R 0 1 , D ate=N o v-12) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 -0.1468 1 .4 7 0 2 5 Unequal 6.41 -0 .1 4 6 8 2 .1 3 3 3 6 8 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .4 0 9 4 Standard Error 0 .8 9 0 2 8 0 4 0 .9 3 1 9 8 4 7 95.0% LCL Difference -2 .1 6 0 7 5 4 -2 .3 9 2 3 0 3 95.0% UCL Difference 1 .8 6 7 1 5 4 2.09 8 7 0 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .1 6 4 9 0 .8 7 2 6 7 3 No D ifference < 0 -0 .1 6 4 9 0 .4 3 6 3 3 7 No D ifference > 0 -0 .1 6 4 9 0 .5 6 3 6 6 3 No Difference: (R e a c h = R 0 1 , D ate= Ju l-09)-(R each = R 01, D ate=N o v-12) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 5 2 5 1 7 0 .0 6 7 8 6 0 0 .0 3 6 0 9 6 Power (Alpha=.010) 0 .0 1 0 7 1 1 0 .0 1 4 4 8 4 0 .0 0 6 7 7 4 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .1 5 7 5 0 .8 7 9 6 8 5 No D ifference < 0 -0 .1 5 7 5 0 .4 3 9 8 4 3 No D ifference > 0 -0 .1 5 7 5 0 .5 6 0 1 5 7 No Difference: (R e a c h = R 01 , D ate = J u l-0 9 )-(R e a c h = R 0 1 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 5 2 1 0 6 0 .0 6 6 4 0 6 0 .0 3 6 9 8 4 Power (Alpha=.010) 0 .0 1 0 5 6 4 0 .0 1 3 9 8 5 0 .0 0 7 0 3 5 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-09) Skew ness Norm ality (R e a c h = R 01 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 01 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 2 .6 4 1 9 M odified-Levene E qual-V ariance Test 0.1411 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .3 1 5 3 4 1 0 .7 1 5 9 0 3 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-09 Reach=R01, Date=Nov-12 Count 6 5 Median 1.485 1.5 95.0% LCL of Median 0 .9 95.0% UCL of Median 3 .7 7 4/10/2013 11:15:15 AM 215 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 01, D ate=Jul-09 R each=R 01, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 17 38 13 28 Multiplicity F acto r = 0 Mean of W 36 30 Std Dev of W 5 .4 7 7 2 2 6 5 .4 7 7 2 2 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.792208 No 0.603896 No 0.396104 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.3651 0.715001 No -0.3651 0.642500 No -0.3651 0.357500 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0 .2 7 3 9 0.784191 No -0 .4 5 6 4 0.675962 No -0 .2 7 3 9 0.392096 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .2 0 0 0 0 0 0 .4 0 0 0 0 0 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .6 8 8 3 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal ProbabilityPlot of Channel_Catfish_PFGS_ppbwhenReach=R01,Date=Jul 4/10/2013 11:15:15 AM 216 Channel_Catfish_PFOS_ppbvs Groups 5.5-| 4/10/2013 11:15:45 AM 415 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, D ate=Jun-02 6 1 5 .2 3 3 3 3 1 3 .9 8 0 0 8 5 .7 0 7 3 4 4 0 .5 6 2 1 3 8 2 9 .9 0 4 5 3 R each=R 02, D ate=N ov-04 5 3 .8 1 5 2 2 .1 3 8 9 5 9 0 .9 5 6 5 7 1 5 1 .1 5 9 3 3 2 6 .4 7 1 0 6 8 Note: T-alpha (R each =R 02, D ate=Jun-02) = 2 .5706, T-alpha (R each =R 02, D ate=N o v-04) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 11.41813 10.51725 Unequal 5 .2 8 1 1 .4 1 8 1 3 1 4 .1 4 2 7 7 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .5 3 0 2 Standard Error 6.36 8 5 1 4 5 .7 8 6 9 5 2 95.0% LCL Difference -2 .9 8 8 4 4 7 -3 .2 2 3 8 0 3 95.0% UCL Difference 2 5 .8 2 4 7 1 26.0 6 0 0 7 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .7 9 2 9 0 .1 0 6 5 8 1 No D ifference < 0 1 .7 9 2 9 0 .9 4 6 7 1 0 No D ifference > 0 1 .7 9 2 9 0 .0 5 3 2 9 0 No O o Z Difference: (R e a c h = R 02 , D ate=Ju n -02)-(R each =:R 02, Date= T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .3 6 0 7 3 3 0 .0 0 0 4 5 8 0 .5 0 4 6 4 8 Power (Alpha=.010) 0 .1 3 7 0 5 7 0 .0 0 0 0 4 9 0 .2 1 3 8 4 4 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1.9731 0 .1 0 2 4 7 1 No D ifference < 0 1.9731 0 .9 4 8 7 6 4 No D ifference > 0 1.9731 0 .0 5 1 2 3 6 No Difference: (R e a c h = R 02 , D a te = J u n -0 2 )-(R e a c h = R 0 2 , D ate=N o v-04) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .3 6 7 1 5 7 0 .0 0 0 3 3 0 0 .5 2 8 0 6 5 Power (Alpha=.010) 0 .1 2 4 0 2 7 0 .0 0 0 0 4 3 0 .2 0 4 9 7 4 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 2 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 02 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 02 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 02 , D ate= N o v-04) Variance-R atio E qual-V ariance Test 4 2 .7 1 8 4 M odified-Levene E qual-V ariance Test 6 .7 6 9 3 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 2 9 0 3 0 .0 2 8 6 5 4 R eject equal variances R eject equal variances Median Statistics Variable Reach=R02, Date=Jun-02 Reach=R02, Date=Nov-04 Count 6 5 Median 14.35 4.36 95.0% LCL of Median 1 95.0% UCL of Median 3 7 .4 4/10/2013 11:15:46 AM 416 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, Date=Jun-02 R each=R 02, D ate=N ov-04 N u m b e r S ets o f T ie s = 1, Mann W Whitney U Sum Ranks 22 43 8 23 Multiplicity F acto r = 6 Mean of W 36 30 Std Dev of W 5 .4 6 4 7 6 3 5 .4 6 4 7 6 3 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1 .2 8 0 9 0.200217 No -1 .2 8 0 9 0.899892 No -1 .2 8 0 9 0.100108 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1 .1 8 9 4 0.234267 No -1 .3 7 2 4 0.915035 No -1 .1 8 9 4 0.117134 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 6 6 6 6 7 0 .1 3 3 3 3 3 0 .6 6 6 6 6 7 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .1 0 8 2 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R02, Date=J 4/10/2013 11:15:46 AM 417 Channel_Catflsh_PFOS_ppbvs Groups 40n 4/10/2013 11:15:46 AM 418 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, D ate=Jun-02 6 1 5 .2 3 3 3 3 1 3 .9 8 0 0 8 5 .7 0 7 3 4 4 0 .5 6 2 1 3 8 2 9 .9 0 4 5 3 R each=R 02, D ate=D ec-06 5 6 .0 9 4 2 .9 8 9 7 9 6 1 .3 3 7 0 7 7 2 .3 8 1 6 7 8 9 .8 0 6 3 2 2 Note: T-alpha (R each =R 02, D ate=Jun-02) = 2 .5706, T-alpha (R each =R 02, D ate=D ec-06) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 9.139334 1 0 .6 0 9 0 6 Unequal 5.54 9 .139334 1 4 .2 9 6 2 1 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .4 9 6 7 Standard Error 6.42 4 1 0 3 5 .8 6 1 8 7 3 95.0% LCL Difference -5 .3 9 2 9 9 8 -5 .4 9 5 7 2 7 95.0% UCL Difference 23.6 7 1 6 7 23.7 7 4 3 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .4 2 2 7 0 .1 8 8 5 5 6 No D ifference < 0 1 .4 2 2 7 0 .9 0 5 7 2 2 No D ifference > 0 1 .4 2 2 7 0 .0 9 4 2 7 8 No Difference: (R e a c h = R 02 , D ate=Ju n -02)-(R each =:R 02, Date= D ec-06) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 4 6 8 8 4 0 .0 0 1 4 9 3 0 .3 7 0 9 7 2 Power (Alpha=.010) 0 .0 8 1 3 8 8 0.000181 0 .1 3 4 1 4 4 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1.5591 0 .1 7 4 0 1 5 No D ifference < 0 1.5591 0 .9 1 2 9 9 3 No D ifference > 0 1.5591 0 .0 8 7 0 0 7 No Difference: (R e a c h = R 02 , D a te = J u n -0 2 )-(R e a c h = R 0 2 , D a te = D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 5 4 5 5 0 0 .0 0 1 1 8 4 0 .3 9 1 1 5 6 Power (Alpha=.010) 0 .0 7 6 9 2 4 0 .0 0 0 1 6 3 0 .1 3 2 2 4 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 2 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 02 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 02 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 02 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 2 1 .8 6 4 3 M odified-Levene E qual-V ariance Test 5 .4 8 8 2 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 1 0 5 2 0 0 .0 4 3 8 2 7 R eject equal variances R eject equal variances Median Statistics Variable Reach=R02, Date=Jun-02 Reach=R02, Date=Dec-06 Count 6 5 Median 14.35 4.88 95.0% LCL of Median 1 95.0% UCL of Median 3 7 .4 4/10/2013 11:15:46 AM 419 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, Date=Jun-02 R each=R 02, D ate=D ec-06 N u m b e r S ets o f T ie s = 1, Mann W Whitney U Sum Ranks 2 0 41 10 25 Multiplicity F acto r = 6 Mean of W 36 30 Std Dev of W 5 .4 6 4 7 6 3 5 .4 6 4 7 6 3 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0 .9 1 5 0 0.360216 No -0 .9 1 5 0 0.819892 No -0 .9 1 5 0 0.180108 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0 .8 2 3 5 0.410248 No -1 .0 0 6 4 0.842900 No -0 .8 2 3 5 0.205124 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 6 6 6 6 7 0 .3 3 3 3 3 3 0 .6 6 6 6 6 7 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .1 0 8 2 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R02, Date=J 4/10/2013 11:15:46 AM 420 Channel_Catflsh_PFOS_ppbvs Groups 40n 4/10/2013 11:15:46 AM 421 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, D ate=Jun-02 6 1 5 .2 3 3 3 3 1 3 .9 8 0 0 8 5 .7 0 7 3 4 4 0 .5 6 2 1 3 8 2 9 .9 0 4 5 3 R each=R 02, D ate=N ov-12 5 2 .4 1 2 0 .8 5 8 2 9 4 8 0 .3 8 3 8 4 1 1 1 .3 4 6 2 8 6 3 .4 7 7 7 1 4 Note: T-alpha (R each =R 02, D ate=Jun-02) = 2 .5706, T-alpha (R each =R 02, D ate=N o v-12) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 12.82133 10.43584 Unequal 5 .0 5 1 2 .8 2 1 3 3 1 4 .0 0 6 4 Note: T-alpha (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .5 6 3 7 Standard Error 6.31 9 2 1 2 5 .7 2 0 2 3 7 95.0% LCL Difference -1 .4 7 3 7 1 8 -1 .8 4 3 4 6 5 95.0% UCL Difference 27.1 1 6 3 8 2 7 .4 8 6 1 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .0 2 8 9 0 .0 7 3 0 5 9 No D ifference < 0 2 .0 2 8 9 0.963471 No D ifference > 0 2 .0 2 8 9 0 .0 3 6 5 2 9 Yes D iffe re n c e : (R e a c h = R 0 2 , D a te = J u n -0 2 )-(R e a c h = :R 0 2 , D a te = :N o v -1 2 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 4 1 4 2 3 0 .0 0 0 2 0 3 0 .5 9 0 4 4 8 Power (Alpha=.010) 0 .1 8 3 6 0 4 0 .0 0 0 0 2 0 0 .2 7 6 3 2 5 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .2 4 1 4 0 .0 7 4 6 0 4 No D ifference < 0 2 .2 4 1 4 0 .9 6 2 6 9 8 No D ifference > 0 2 .2 4 1 4 0 .0 3 7 3 0 2 Yes Difference: (R e a c h = R 02 , D a te = J u n -0 2 )-(R e a c h = R 0 2 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 4 3 2 9 7 0 .0 0 0 1 3 6 0 .6 1 2 6 6 3 Power (Alpha=.010) 0 .1 5 9 7 0 9 0 .0 0 0 0 1 7 0 .2 5 7 9 9 6 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 2 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 02 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 02 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 02 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 2 6 5 .3 0 5 6 M odified-Levene E qual-V ariance Test 8 .1 9 9 7 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 7 9 0 .0 1 8 6 7 3 R eject equal variances R eject equal variances Median Statistics Variable Reach=R02, Date=Jun-02 Reach=R02, Date=Nov-12 Count 6 5 Median 14.35 2.65 95.0% LCL of Median 1 95.0% UCL of Median 3 7 .4 4/10/2013 11:15:46 AM 422 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, Date=Jun-02 R each=R 02, D ate=N ov-12 N u m b e r S ets o f T ie s = 1, Mann W Whitney U Sum Ranks 2 0 41 10 25 Multiplicity F acto r = 6 Mean of W 36 30 Std Dev of W 5 .4 6 4 7 6 3 5 .4 6 4 7 6 3 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0 .9 1 5 0 0.360216 No -0 .9 1 5 0 0.819892 No -0 .9 1 5 0 0.180108 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0 .8 2 3 5 0.410248 No -1 .0 0 6 4 0.842900 No -0 .8 2 3 5 0.205124 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 6 6 6 6 7 0 .3 3 3 3 3 3 0 .6 6 6 6 6 7 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .1 0 8 2 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R02, Date=J 4/10/2013 11:15:47 AM 423 Channel_Catflsh_PFOS_ppbvs Groups 40n 4/10/2013 11:16:00 AM 511 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, D ate=N ov-04 5 3 .8 1 5 2 2.13 8 9 5 9 0 .9 5 6 5 7 1 5 1 .1 5 9 3 3 2 6 .4 7 1 0 6 8 R each=R 02, D ate=D ec-06 5 6 .0 9 4 2 .9 8 9 7 9 6 1 .3 3 7 0 7 7 2.38 1 6 7 8 9 .8 0 6 3 2 2 Note: T-alp h a (R each = R 02, D ate=N o v-04) = 2 .7 7 6 4 , T-alpha (R each = R 02, D a te= D ec-06) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -2.2788 2 .5 9 9 4 2 6 Unequal 7.24 -2.2 7 8 8 3 .6 7 6 1 4 3 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .3 4 8 5 Standard Error 1 .6 4 4 0 2 1 1 .6 4 4 0 2 1 95.0% LCL Difference -6 .0 6 9 9 1 9 -6 .1 3 9 8 4 95.0% UCL Difference 1 .5 1 2 3 1 9 1 .5 8 2 2 4 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1.3861 0 .2 0 3 1 2 1 No D ifference < 0 -1.3861 0 .1 0 1 5 6 0 No D ifference > 0 -1.3861 0 .8 9 8 4 4 0 No D iffe re n c e : (R e a c h = R 0 2 , D a te = N o v -0 4 )-(R e a c h = R 0 2 , D a te := D e c -0 6 ) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 3 1 4 9 4 0 .3 5 3 4 9 9 0 .0 0 1 7 2 7 Power (Alpha=.010) 0 .0 7 3 2 4 3 0 .1 2 2 8 2 9 0 .0 0 0 2 1 9 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1.3861 0 .2 0 6 8 7 6 No D ifference < 0 -1.3861 0 .1 0 3 4 3 8 No D ifference > 0 -1.3861 0 .8 9 6 5 6 2 No D iffe re n c e : (R e a c h = R 0 2 , D a te = N o v -0 4 )-(R e a c h = R 0 2 , D a te := D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 2 6 6 2 2 0 .3 4 9 0 1 8 0 .0 0 1 7 8 2 Power (Alpha=.010) 0 .0 6 9 9 5 5 0 .1 1 8 6 0 3 0 .0 0 0 2 3 3 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D ate= N ov-04) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 02 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 02 , D ate= D ec-06) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 02 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 1 .9 5 3 8 M odified-Levene E qual-V ariance Test 0 .4 7 4 9 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .5 3 2 4 7 8 0 .5 1 0 2 4 9 C annot reject equal variances C annot reject equal variances Median Statistics Variable R each=R 02, D ate=N ov-04 R each=R 02, D ate=D ec-06 Count 5 5 Median 4 .3 6 4 .8 8 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:16:00 AM 512 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, D ate=N ov-04 R each=R 02, D ate=D ec-06 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 9 24 16 31 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.273810 No 0.726191 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.7311 0.464702 No -0.7311 0.232351 No -0.7311 0.767649 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0 .6 2 6 7 0.530869 No -0 .6 2 6 7 0.265435 No -0 .8 3 5 6 0.798302 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .4 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .8 7 3 0 Plots Section Histogram 60 -, 60 - , --------------------------------- Histogram 50- 50 - 40 - 40 - 20 - 10 - 0 --------------,---------------------------- 1---------------------------- ,-------------- 01 234S6 Channel_Catfish_PFOS_ppb when F;each=R02, Date=Nov-04 20 - 10 - 0-L|-------------- -------------------------------------------- 3 5 7 3 11 Channel_Catfish_PFOS_ppb when Reach=R02, Date=Dec-06 Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=RG2, Date= 4/10/2013 11:16:00 AM 513 11.00n Channel_Catfish_PFOS_ppbvs Groups 4/10/2013 11:16:01 AM 514 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, D ate=N ov-04 5 3 .8 1 5 2 2 .1 3 8 9 5 9 0 .9 5 6 5 7 1 5 1 .1 5 9 3 3 2 6 .4 7 1 0 6 8 R each=R 02, D ate=N ov-12 5 2 .4 1 2 0 .8 5 8 2 9 4 8 0 .3 8 3 8 4 1 1 1 .3 4 6 2 8 6 3 .4 7 7 7 1 4 Note: T-alpha (R e a c h =R02, D ate=N o v-04) = 2 .7764, T-alpha (R each =R 02, D ate=N o v-12) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 1.4032 1 .6 2 9 6 9 6 Unequal 5 .2 6 1 .4 0 3 2 2 .3 0 4 7 3 8 Note: T-alpha (Equal) = 2 .3060, T-alpha (U nequal) = 2 .5334 Standard Error 1.03071 1.03071 95.0% LCL Difference -0 .9 7 3 6 2 1 4 -1 .2 0 8 0 3 6 95.0% UCL Difference 3 .7 8 0 0 2 1 4 .0 1 4 4 3 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .3 6 1 4 0 .2 1 0 4 9 3 No D ifference < 0 1 .3 6 1 4 0 .8 9 4 7 5 4 No D ifference > 0 1 .3 6 1 4 0 .1 0 5 2 4 6 No D iffe re n c e : (R e a c h = R 0 2 , D a te = N o v -0 4 )-( R e a c h := R 0 2 , D ate=:N o v -1 2 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 2 5 0 0 5 0 .0 0 1 8 5 8 0 .3 4 5 1 5 6 Power (Alpha=.010) 0 .0 7 0 5 4 6 0 .0 0 0 2 3 7 0 .1 1 8 6 9 6 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .3 6 1 4 0 .2 2 8 8 5 2 No D ifference < 0 1 .3 6 1 4 0 .8 8 5 5 7 4 No D ifference > 0 1 .3 6 1 4 0 .1 1 4 4 2 6 No Difference: (R e a c h = R 02 , D a te = N o v -0 4 )-(R e a c h = R 0 2 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 0 2 5 9 9 0 .0 0 2 1 5 4 0 .3 2 3 7 7 6 Power (Alpha=.010) 0 .0 5 6 7 0 9 0 .0 0 0 3 1 3 0 .1 0 0 1 9 9 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 02 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 02 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 02 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 6 .2 1 0 6 M odified-Levene E qual-V ariance Test 1 .1 8 8 6 Probability 0.0000 Decision(.050) 1.000000 C annot reject norm ality 0.0000 1.000000 C annot reject norm ality 0 .1 0 4 7 3 2 0 .3 0 7 3 5 3 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R02, Date=Nov-04 Reach=R02, Date=Nov-12 Count 5 5 Median 4.36 2.65 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:16:01 AM 515 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, D ate=N ov-04 R each=R 02, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 35 5 20 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.924603 No 0.075397 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1 .5 6 6 7 0.117185 No 1 .5 6 6 7 0.941407 No 1 .5 6 6 7 0.058593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1 .4 6 2 3 0.143672 No 1.6711 0.952654 No 1 .4 6 2 3 0.071836 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .8 0 0 0 0 0 0 .2 0 0 0 0 0 0 .8 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 7 9 4 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:16:01 AM 516 4/10/2013 11:16:15 AM 604 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, D ate=D ec-06 5 6 .0 9 4 2 .9 8 9 7 9 6 1 .3 3 7 0 7 7 2 .3 8 1 6 7 8 9 .8 0 6 3 2 2 R each=R 02, D ate=N ov-12 5 2 .4 1 2 0 .8 5 8 2 9 4 8 0 .3 8 3 8 4 1 1 1 .3 4 6 2 8 6 3 .4 7 7 7 1 4 Note: T-alp h a (R each = R 02, D ate= D ec-06) = 2 .7 7 6 4 , T-alpha (R each = R 02, D ate=N o v-12) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 3.682 2 .1 9 9 4 9 4 Unequal 4.65 3.682 3 .1 1 0 5 5 5 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .6 2 9 0 Standard Error 1 .3 9 1 0 8 2 1 .3 9 1 0 8 2 95.0% LCL Difference 0 .4 7 4 1 5 8 4 0 .0 2 4 8 6 7 3 6 95.0% UCL Difference 6.88 9 8 4 2 7 .3 3 9 1 3 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .6 4 6 9 0 .0 2 9 3 9 9 Yes D ifference < 0 2 .6 4 6 9 0.985301 No D ifference > 0 2 .6 4 6 9 0 .0 1 4 6 9 9 Yes Difference: (R e a c h = R 0 2 , D ate= D e c -0 6 )-(R e a c h =R 02, Date=:N ov-12) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .6 4 1 6 2 0 0.000021 0 .7 7 9 1 3 0 Power (Alpha=.010) 0 .3 2 3 5 7 0 0 .0 0 0 0 0 2 0 .4 5 1 3 8 0 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .6 4 6 9 0 .0 4 8 9 8 0 Yes D ifference < 0 2 .6 4 6 9 0 .9 7 5 5 1 0 No D ifference > 0 2 .6 4 6 9 0 .0 2 4 4 9 0 Yes Difference: (R e a c h = R 02 , D a te = D e c -0 6 )-(R e a c h = R 0 2 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .5 5 3 5 8 5 0 .0 0 0 0 3 3 0 .7 2 4 7 2 8 Power (Alpha=.010) 0 .2 1 7 1 3 8 0 .0 0 0 0 0 4 0 .3 4 0 6 1 5 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 02 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 02 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 02 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1 2 .1 3 4 2 M odified-Levene E qual-V ariance Test 2 .6 7 8 6 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 3 3 0 1 6 0 .1 4 0 3 4 5 R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R02, Date=Dec-06 Reach=R02, Date=Nov-12 Count 5 5 Median 4.88 2.65 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:16:15 AM 605 Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, D ate=D ec-06 R each=R 02, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 39 1 16 Multiplicity F a c to r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.992063 No 0.007937 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2 .4 0 2 3 0.016294 Yes 2 .4 0 2 3 0.991853 No 2 .4 0 2 3 0.008147 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2 .2 9 7 8 0.021572 Yes 2 .5 0 6 7 0.993907 No 2 .2 9 7 8 0.010786 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .8 0 0 0 0 0 0 .0 0 0 0 0 0 0 .8 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 7 9 4 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R02, Date=D 4/10/2013 11:16:15 AM 606 Channel_Catflsh_PFOS_ppb vs Groups 11 i Reach=R02, Date=Dec-06 Reach=R02,Date=Nov-12 Groups 4/10/2013 11:17:11 AM 946 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=N ov-04 10 410.1 1 8 8 .3 1 5 5 9 .5 5 0 4 4 2 7 5 .3 8 7 5 5 4 4 .8 1 2 4 R each=R 05, D ate=D ec-06 10 6 9 6 .8 3 8 2 .4 6 8 1 2 0 .9 4 7 4 2 3 .1 9 8 9 9 7 0 .4 0 1 1 Note: T-alpha (R each =R 05, D ate=N o v-04) = 2 .2 6 2 2 , T-alpha (R each = R 05, D a te= D ec-06) = 2 .2622 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 18 -286.7 3 0 1 .4 5 0 1 Unequal 13.12 -286.7 4 2 6 .3 1 4 8 Note: T-alpha (Equal) = 2 .1 0 0 9 , T-alpha (U nequal) = 2 .1 5 8 3 Standard Error 1 3 4 .8 1 2 6 1 3 4 .8 1 2 6 95.0% LCL Difference -5 6 9 .9 3 0 7 -577.671 95.0% UCL Difference -3 .4 6 9 3 1 4 .2 7 1 0 4 1 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .1 2 6 7 0 .0 4 7 5 3 6 Yes D ifference < 0 -2 .1 2 6 7 0 .0 2 3 7 6 8 Yes D ifference > 0 -2 .1 2 6 7 0 .9 7 6 2 3 2 No Difference: (R e a c h = R 05 , D a te = N o v -0 4 )-(R e a c h : D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .5 2 1 0 4 1 0 .6 5 5 7 3 5 0 .0 0 0 1 1 0 Power (Alpha=.010) 0 .2 6 0 5 8 7 0 .3 6 0 0 6 5 0 .0 0 0 0 0 8 II II ZoJ ZoJ cn en DCD DCD ilCD ilCD Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .1 2 6 7 0 .0 5 2 9 8 3 No D ifference < 0 -2 .1 2 6 7 0 .0 2 6 4 9 2 Yes D ifference > 0 -2 .1 2 6 7 0 .9 7 3 5 0 8 No Difference: (R e a c h = R 05 , D a te = N o v -0 4 )-(R e a c h : D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .5 0 4 1 3 6 0 .6 4 4 4 3 3 0 .0 0 0 1 2 2 Power (Alpha=.010) 0 .2 3 8 7 1 5 0 .3 3 8 6 7 6 0 .0 0 0 0 1 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 4 .1 2 5 0 M odified-Levene E qual-V ariance Test 1 0 .7 4 6 9 Probability -0 .5 9 0 5 -0 .8 4 9 7 1 .0 7 0 7 -0 .0 8 0 4 -1 .8 9 5 7 3.6001 0 .0 4 6 3 9 2 0 .0 0 4 1 7 7 Decision(.050) 0 .5 5 4 8 6 5 C annot reject norm ality 0 .3 9 5 4 7 6 C annot reject norm ality 0 .5 8 5 4 6 1 C annot reject norm ality 0 .9 3 5 8 8 0 C annot reject norm ality 0 .0 5 8 0 0 1 C annot reject norm ality 0 .1 6 5 2 9 0 C annot reject norm ality R eject equal variances R eject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Dec-06 Count 10 10 Median 404 747 95.0% LCL of Median 147 257 95.0% UCL of Median 608 1070 4/10/2013 11:17:11 AM 947 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=N ov-04 R each=R 05, D ate=D ec-06 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 30 85 70 125 Multiplicity Factor = 0 Mean of W 105 105 Std Dev of W 1 3 .2 2 8 7 6 1 3 .2 2 8 7 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.143140 No 0.071570 No 0.928430 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1 .5 1 1 9 0.130570 No -1 .5 1 1 9 0.065285 No -1 .5 1 1 9 0.934715 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.4741 0.140465 No -1.4741 0.070233 No -1 .5 4 9 7 0.939388 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .5 0 0 0 0 0 0 .5 0 0 0 0 0 0 .1 0 0 0 0 0 Reject H0 if Greater Than 0 .5 6 2 3 0 .5 6 2 3 0 .5 6 2 3 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .1 6 7 8 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R05, Date=No 4/10/2013 11:17:11 AM 948 1400n 1200- Channel_Catfish_PFOS_ppb vs Groups 200- 0J---------------- 1--------------------------------- i---------- Reach=R05, Date=Nev-04 Reach=R05, Date=Dec-06 Groups 4/10/2013 11:17:12 AM 949 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=N ov-04 10 410.1 1 8 8 .3 1 5 5 9 .5 5 0 4 4 2 7 5 .3 8 7 5 5 4 4 .8 1 2 4 R each=R 05, D ate=Jul-07 12 1 2 1 .9 5 8 3 2 1 4 .5 9 3 6 1 .9 4 7 6 7 -1 4 .3 8 7 5 7 2 5 8 .3 0 4 2 Note: T-alpha (R each =R 05, D ate=N o v-04) = 2 .2622, T-alpha (R each =R 05, D ate=Jul-07) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 288.1417 2 0 3 .1 8 8 9 Unequal 19.93 2 8 8 .1 4 1 7 2 8 5 .5 0 4 3 Note: T-alpha (Equal) = 2 .0 8 6 0 , T-alpha (U nequal) = 2 .0 8 6 5 Standard Error 8 7 .0 0 0 3 8 5 .9 2 8 8 6 95.0% LCL Difference 1 0 6 .6 6 2 2 1 0 8 .8 5 4 6 95.0% UCL Difference 4 6 9 .6 2 1 1 4 6 7 .4 2 8 7 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 3 .3 1 2 0 0 .0 0 3 4 8 0 Yes D ifference < 0 3 .3 1 2 0 0 .9 9 8 2 6 0 No D ifference > 0 3 .3 1 2 0 0 .0 0 1 7 4 0 Yes D ifference: (R e a c h = R 0 5 , D ate= N o v -0 4 )-(R e a c h =R 05, Date= Jul-07) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0.882841 0.000001 0 .9 3 9 6 6 8 Power (Alpha=.010) 0 .6 7 7 1 3 8 0 .0 0 0 0 0 0 0 .7 7 5 8 8 8 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 3 .3 5 3 3 0 .0 0 3 1 7 7 Yes D ifference < 0 3 .3 5 3 3 0 .9 9 8 4 1 2 No D ifference > 0 3 .3 5 3 3 0 .0 0 1 5 8 8 Yes Difference: (R e a c h = R 05 , D a te = N o v -0 4 )-(R e a c h = R 0 5 , D ate=Ju l-07) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .8 9 0 2 4 9 0.000001 0 .9 4 4 2 3 4 Power (Alpha=.010) 0 .6 9 0 1 6 1 0 .0 0 0 0 0 0 0.786931 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-07) Variance-R atio E qual-V ariance Test 1 .2 9 8 6 M odified-Levene E qual-V ariance Test 0.2491 Probability -0 .5 9 0 5 -0 .8 4 9 7 1 .0 7 0 7 2 .7 6 6 2 1 .8 9 3 4 1 1 .2 3 6 8 0 .7 0 6 0 8 8 0 .6 2 3 1 3 3 Decision(.050) 0 .5 5 4 8 6 5 C annot reject norm ality 0 .3 9 5 4 7 6 C annot reject norm ality 0.585461 C annot reject norm ality 0 .0 0 5 6 7 2 R eject norm ality 0 .0 5 8 3 0 5 C annot reject norm ality 0.003631 R eject norm ality C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Jul-07 Count 10 12 Median 404 8.65 95.0% LCL of Median 147 7 .3 95.0% UCL of Median 608 302.1 4/10/2013 11:17:12 AM 950 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=N ov-04 R each=R 05, D ate=Jul-07 N u m b e r S ets o f T ie s = 1, Mann W Whitney U Sum Ranks 102 157 18 96 M u ltip licity F a c to r= 6 Mean of W 115 138 Std Dev of W 1 5 .1 6 1 4 7 1 5 .1 6 1 4 7 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2 .7 7 0 2 0.005603 Yes 2 .7 7 0 2 0.997199 No 2 .7 7 0 2 0.002801 Y es Approx. With Correction Prob Reject H0 Z-Value Level at .050 2 .7 3 7 2 0.006196 Yes 2 .8 0 3 2 0.997470 No 2 .7 3 7 2 0.003098 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .7 5 0 0 0 0 0 .0 8 3 3 3 3 0 .7 5 0 0 0 0 Reject H0 if Greater Than 0 .5 4 0 8 0 .5 4 0 8 0 .5 4 0 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 0 1 4 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:12 AM 951 4/10/2013 11:17:12 AM 952 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=N ov-04 10 410.1 1 8 8 .3 1 5 5 9 .5 5 0 4 4 2 7 5 .3 8 7 5 5 4 4 .8 1 2 4 R each=R 05, D ate=Jul-08 12 1 2 2 .0 6 4 2 1 7 0 .6 1 0 7 4 9 .2 5 1 0 8 1 3 .6 6 3 2 8 2 3 0 .4 6 5 1 Note: T-alpha (R each =R 05, D ate=N o v-04) = 2 .2622, T-alpha (R each =R 05, D ate=Jul-08) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 288.0358 1 7 8 .7 9 4 7 Unequal 18.46 28 8 .0 3 5 8 2 5 4 .1 0 7 4 Note: T-alp h a (Equal) = 2 .0 8 6 0 , T-alpha (U nequal) = 2 .0 9 7 2 Standard Error 7 6 .5 5 5 3 3 7 7 .2 7 8 2 1 95.0% LCL Difference 1 2 8 .3 4 4 2 1 2 5 .9 6 8 3 95.0% UCL Difference 4 4 7 .7 2 7 4 4 5 0 .1 0 3 4 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 3 .7 6 2 5 0 .0 0 1 2 2 5 Yes D ifference < 0 3 .7 6 2 5 0 .9 9 9 3 8 7 No D ifference > 0 3 .7 6 2 5 0 .0 0 0 6 1 3 Yes D ifference: (R e a c h = R 0 5 , D ate= N o v -0 4 )-(R e a c h =R 05, Date= Jul-08) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 4 6 9 9 8 0 .0 0 0 0 0 0 0 .9 7 6 4 8 6 Power (Alpha=.010) 0 .8 0 7 8 4 2 0 .0 0 0 0 0 0 0 .8 8 0 1 9 4 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 3 .7 2 7 3 0 .0 0 1 4 8 8 Yes D ifference < 0 3 .7 2 7 3 0 .9 9 9 2 5 6 No D ifference > 0 3 .7 2 7 3 0 .0 0 0 7 4 4 Yes Difference: (R e a c h = R 05 , D a te = N o v -0 4 )-(R e a c h = R 0 5 , D ate=Ju l-08) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 4 1 4 7 3 0 .0 0 0 0 0 0 0 .9 7 3 8 6 6 Power (Alpha=.010) 0 .7 9 1 4 9 6 0 .0 0 0 0 0 0 0 .8 6 8 9 6 4 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-08) Variance-R atio E qual-V ariance Test 1 .2 1 8 3 M odified-Levene E qual-V ariance Test 0 .6 3 8 3 Probability -0 .5 9 0 5 -0 .8 4 9 7 1 .0 7 0 7 2 .1 0 0 8 0 .3 1 3 6 4 .5 1 1 9 0 .7 4 5 0 4 7 0 .4 3 3 7 1 4 Decision(.050) 0 .5 5 4 8 6 5 C annot reject norm ality 0 .3 9 5 4 7 6 C annot reject norm ality 0.585461 C annot reject norm ality 0 .0 3 5 6 5 4 R eject norm ality 0 .7 5 3 8 2 9 C annot reject norm ality 0 .1 0 4 7 7 4 C annot reject norm ality C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Jul-08 Count 10 12 Median 404 43.115 95.0% LCL of Median 147 9 .1 2 95.0% UCL of Median 608 3 6 5 .8 4/10/2013 11:17:12 AM 953 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=N ov-04 R each=R 05, D ate=Jul-08 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 106 161 14 92 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 1 5 .1 6 5 7 5 1 5 .1 6 5 7 5 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.001519 Yes 0.999241 No 0.000759 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 3 .0 3 3 2 0.002420 Yes 3 .0 3 3 2 0.998790 No 3 .0 3 3 2 0.001210 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 3 .0 0 0 2 0.002698 Yes 3.0661 0.998916 No 3 .0 0 0 2 0.001349 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .7 5 0 0 0 0 0 .0 0 0 0 0 0 0 .7 5 0 0 0 0 Reject H0 if Greater Than 0 .5 4 0 8 0 .5 4 0 8 0 .5 4 0 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 0 1 4 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R05, Date=No 4/10/2013 11:17:12 AM 954 1 650 5 10 25 50 75 Percent of Values 90 95 99 Channel_Catfish_PFOS_ppb vs Groups Reach=R05, Date=Nov-04 Reach=R05, Date-Jul-08 Groups 4/10/2013 11:17:13 AM 955 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=N ov-04 10 410.1 1 8 8 .3 1 5 5 9 .5 5 0 4 4 2 7 5 .3 8 7 5 5 4 4 .8 1 2 4 R each=R 05, D ate=Jul-09 12 1 8 2 .8 1 8 3 1 7 1 .4 0 4 5 4 9 .4 8 0 2 2 7 3 .9 1 3 0 9 2 9 1 .7 2 3 6 Note: T-alpha (R each =R 05, D ate=N o v-04) = 2 .2622, T-alpha (R each =R 05, D ate=Jul-09) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 227.2817 1 7 9 .2 1 1 8 Unequal 18.50 22 7 .2 8 1 7 2 5 4 .6 4 1 Note: T-alpha (Equal) = 2 .0 8 6 0 , T-alpha (U nequal) = 2 .0 9 6 8 Standard Error 7 6 .7 3 3 9 2 7 7 .4 2 4 4 6 95.0% LCL Difference 67.2 1 7 5 2 6 4 .9 3 4 4 2 95.0% UCL Difference 3 8 7 .3 4 5 8 3 8 9 .6 2 8 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .9 6 1 9 0 .0 0 7 7 0 8 Yes D ifference < 0 2 .9 6 1 9 0 .9 9 6 1 4 6 No D ifference > 0 2 .9 6 1 9 0 .0 0 3 8 5 4 Yes D ifference: (R e a c h = R 0 5 , D ate= N o v -0 4 )-(R e a c h =R 05, Date= Jul-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .8 0 4 3 4 9 0 .0 0 0 0 0 3 0 .8 8 7 7 4 2 Power (Alpha=.010) 0 .5 5 5 8 7 6 0 .0 0 0 0 0 0 0 .6 6 7 7 1 4 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .9 3 5 5 0 .0 0 8 6 5 8 Yes D ifference < 0 2 .9 3 5 5 0.995671 No D ifference > 0 2 .9 3 5 5 0 .0 0 4 3 2 9 Yes Difference: (R e a c h = R 05 , D a te = N o v -0 4 )-(R e a c h = R 0 5 , D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .7 9 4 0 0 9 0 .0 0 0 0 0 4 0 .8 8 1 1 4 6 Power (Alpha=.010) 0 .5 3 8 5 9 2 0 .0 0 0 0 0 0 0 .6 5 2 7 8 4 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-09) Variance-R atio E qual-V ariance Test 1.2071 M odified-Levene E qual-V ariance Test 0 .0 8 3 2 Probability -0 .5 9 0 5 -0 .8 4 9 7 1 .0 7 0 7 0 .8 2 6 4 -1.2111 2 .1 4 9 8 0 .7 5 5 8 9 6 0 .7 7 6 0 4 0 Decision(.050) 0 .5 5 4 8 6 5 C annot reject norm ality 0 .3 9 5 4 7 6 C annot reject norm ality 0.585461 C annot reject norm ality 0 .4 0 8 5 8 7 C annot reject norm ality 0 .2 2 5 8 4 4 C annot reject norm ality 0 .3 4 1 3 3 9 C annot reject norm ality C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Jul-09 Count 10 12 Median 404 136.38 95.0% LCL of Median 147 1 3 .8 7 95.0% UCL of Median 608 342.21 4/10/2013 11:17:13 AM 956 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=N ov-04 R each=R 05, D ate=Jul-09 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 99 154 21 99 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 1 5 .1 6 5 7 5 1 5 .1 6 5 7 5 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.008957 Yes 0.995522 No 0.004478 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2 .5 7 1 6 0.010123 Yes 2 .5 7 1 6 0.994938 No 2 .5 7 1 6 0.005062 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2 .5 3 8 6 0.011129 Yes 2 .6 0 4 6 0.995400 No 2 .5 3 8 6 0.005565 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .5 3 3 3 3 3 0 .0 0 0 0 0 0 0 .5 3 3 3 3 3 Reject H0 if Greater Than 0 .5 4 0 8 0 .5 4 0 8 0 .5 4 0 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .0 6 1 7 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R05, Date=No 4/10/2013 11:17:13 AM 957 1 650 5 10 25 50 75 Percent of Values 90 95 99 Channel_Catfish_PFOS_ppb vs Groups Reach=R05, Date=Nov-04 Reach=R05, Date-Jul-09 Groups 4/10/2013 11:17:13 AM 958 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=N ov-04 10 410.1 1 8 8 .3 1 5 5 9 .5 5 0 4 4 2 7 5 .3 8 7 5 5 4 4 .8 1 2 4 R each=R 05, D ate=N ov-12 5 256 1 8 8 .5 8 1 5 8 4 .3 3 6 2 2 1 .8 4 5 1 5 4 9 0 .1 5 4 8 Note: T-alpha (R e a c h =R05, D ate=N o v-04) = 2 .2622, T-alpha (R each =R 05, D ate=N o v-12) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 154.1 1 8 8 .3 9 7 Unequal 8 .0 9 154.1 2 6 6 .5 0 6 1 Note: T-alpha (Equal) = 2 .1604, T-alpha (U nequal) = 2 .3 0 1 6 Standard Error 1 0 3 .1 8 9 3 1 0 3 .2 4 1 7 95.0% LCL Difference -6 8 .8 2 6 9 6 -8 3 .5 1 9 0 1 95.0% UCL Difference 3 7 7 .0 2 6 9 3 9 1 .7 1 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .4 9 3 4 0 .1 5 9 2 0 9 No D ifference < 0 1 .4 9 3 4 0 .9 2 0 3 9 5 No D ifference > 0 1 .4 9 3 4 0 .0 7 9 6 0 5 No II ZJ o en D CD iClD Difference: (R e a c h = R 05 , D a te = N o v -0 4 )-(R e a c h : :N ov-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 8 2 7 6 2 0 .0 0 1 0 8 9 0 .4 0 9 2 4 2 Power (Alpha=.010) 0 .1 0 3 1 0 8 0 .0 0 0 1 1 6 0 .1 6 2 6 1 2 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .4 9 2 6 0 .1 7 3 4 7 4 No D ifference < 0 1 .4 9 2 6 0 .9 1 3 2 6 3 No D ifference > 0 1 .4 9 2 6 0 .0 8 6 7 3 7 No Difference: (R e a c h = R 05 , D a te = N o v -0 4 )-(R e a c h = R 0 5 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 6 1 2 5 9 0 .0 0 1 2 4 7 0 .3 9 0 7 2 2 Power (Alpha=.010) 0 .0 8 6 2 0 5 0 .0 0 0 1 5 3 0 .1 4 2 3 1 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1 .0 0 2 8 M odified-Levene E qual-V ariance Test 0 .1 1 2 8 Probability -0 .5 9 0 5 -0 .8 4 9 7 1 .0 7 0 7 0 .0 0 0 0 Decision(.050) 0 .5 5 4 8 6 5 C annot reject norm ality 0 .3 9 5 4 7 6 C annot reject norm ality 0.585461 C annot reject norm ality 1 .0 0 0 0 0 0 C annot reject norm ality 0 .9 0 9 0 4 6 0 .7 4 2 3 0 8 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R05, Date=Nov-12 Count 10 5 Median 404 283 95.0% LCL of Median 147 95.0% UCL of Median 608 4/10/2013 11:17:13 AM 959 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=N ov-04 R each=R 05, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 40 95 10 2 5 Multiplicity F acto r = 0 Mean of W 80 40 Std Dev of W 8 .1 6 4 9 6 6 8 .1 6 4 9 6 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.075258 No 0.962371 No 0.037629 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.8371 0.066193 No -1.8371 0.966904 No -1.8371 0.033096 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1 .7 7 5 9 0.075753 No -1 .8 9 8 4 0.971175 No -1 .7 7 5 9 0.037876 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 0 0 0 0 0 0.000000 0 .6 0 0 0 0 0 Reject H0 if Greater Than 0 .6 6 9 8 0 .6 6 9 8 0 .6 6 9 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .1 6 5 8 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R05, Date=No 4/10/2013 11:17:13 AM 960 1 650 5 10 25 50 75 Percent of Values 90 95 99 Channel_Catfish_PFOS_ppb vs Groups 50J-----------------1---------------------------------- 1---------- Reach=R05, Date=Nov-04 Reach=R05, Date=Nov-12 Groups 4/10/2013 11:17:24 AM 1024 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Dec-06 10 696.8 382.468 120.947 423.1989 970.4011 Reach=R05, Date=Jul-07 12 121.9583 214.593 61.94767 -14.38757 258.3042 Note: T-alpha (Reach=R05, Date=D ec-06) = 2.2622, T-alpha (Reach=R05, Date=Jul-07) = 2.2010 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 574.8417 301.9178 Unequal 13.58 574.8417 438.5566 Note: T-alpha (Equal) = 2.0860, T-alpha (Unequal) = 2.1511 Standard Error 129.2735 135.8885 95.0% LCL Difference 305.1819 282.5355 95.0% UCL Difference 844.5015 867.1478 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 4.4467 0.000248 Yes Difference < 0 4.4467 0.999876 No Difference > 0 4.4467 0.000124 Yes II ZJ o en D CD CD II Difference: (Reach=R05, Date= Dec-06)-(Reach Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.988231 0.000000 0.995916 Power (Alpha=.010) 0.932143 0.000000 0.964799 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 4.2302 0.000896 Yes Difference < 0 4.2302 0.999552 No Difference > 0 4.2302 0.000448 Yes Difference: (Reach=R05, Date=Dec-06)-(Reach=R05, Date=Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.974750 0.000000 0.990926 Power (Alpha=.010) 0.869393 0.000000 0.928668 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Dec-06) Kurtosis Normality (Reach=R05, D ate=Dec-06) Omnibus Normality (Reach=R05, Date=Dec-06) Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Variance-Ratio Equal-Variance Test 3.1766 Modified-Levene Equal-Variance Test 7.7192 Probability -0.0804 -1.8957 3.6001 2.7662 1.8934 11.2368 0.074814 0.011599 Decision(.050) 0.935880 Cannot reject normality 0.058001 Cannot reject normality 0.165290 Cannot reject normality 0.005672 Reject normality 0.058305 Cannot reject normality 0.003631 Reject normality Cannot reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-07 Count 10 12 Median 747 8.65 95.0% LCL of Median 257 7.3 95.0% UCL of Median 1070 302.1 4/10/2013 11:17:24 AM 1025 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-07 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 109 164 11 89 Multiplicity Factor = 6 Mean of W 115 138 Std Dev of W 15.16147 15.16147 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 3.2319 0.001230 Yes 3.2319 0.999385 No 3.2319 0.000615 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 3.1989 0.001380 Yes 3.2649 0.999452 No 3.1989 0.000690 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.750000 0.000000 0.750000 Reject H0 if Greater Than 0.5408 0.5408 0.5408 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0014 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:24 AM 1026 4/10/2013 11:17:25 AM 1027 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=D ec-06 10 6 9 6 .8 3 8 2 .4 6 8 1 2 0 .9 4 7 4 2 3 .1 9 8 9 9 7 0 .4 0 1 1 R each=R 05, D ate=Jul-08 12 1 2 2 .0 6 4 2 1 7 0 .6 1 0 7 4 9 .2 5 1 0 8 1 3 .6 6 3 2 8 2 3 0 .4 6 5 1 Note: T-alpha (R each =R 05, D ate=D ec-06) = 2 .2622, T-alpha (R each =R 05, D ate=Jul-08) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 574.7358 2 8 6 .0 7 0 3 Unequal 11.96 5 7 4.7358 4 1 8 .7 9 5 6 Note: T-alpha (Equal) = 2 .0 8 6 0 , T-alpha (U nequal) = 2 .1 7 9 6 Standard Error 1 2 2 .4 8 8 1 3 0 .5 9 0 4 95.0% LCL Difference 3 1 9 .2 3 0 4 2 9 0 .1 0 6 6 95.0% UCL Difference 8 3 0 .2 4 1 3 8 5 9 .3 6 5 1 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 4 .6 9 2 2 0 .0 0 0 1 4 0 Yes D ifference < 0 4 .6 9 2 2 0 .9 9 9 9 3 0 No D ifference > 0 4 .6 9 2 2 0 .0 0 0 0 7 0 Yes D ifference: (R e a c h = R 0 5 , D ate= D e c -0 6 )-(R e a c h =R 05, Date= Jul-08) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 9 3 7 3 7 0 .0 0 0 0 0 0 0 .9 9 8 0 2 0 Power (Alpha=.010) 0 .9 5 6 8 0 3 0 .0 0 0 0 0 0 0 .9 7 9 1 3 8 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 4.4011 0 .0 0 0 8 7 0 Yes D ifference < 0 4.4011 0 .9 9 9 5 6 5 No D ifference > 0 4.4011 0 .0 0 0 4 3 5 Yes Difference: (R e a c h = R 05 , D a te = D e c -0 6 )-(R e a c h = R 0 5 , D ate=Ju l-08) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 8 0 6 0 3 0 .0 0 0 0 0 0 0 .9 9 3 6 2 3 Power (Alpha=.010) 0 .8 8 4 0 3 2 0 .0 0 0 0 0 0 0 .9 3 9 9 1 8 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-08) Variance-R atio E qual-V ariance Test 5 .0 2 5 5 M odified-Levene E qual-V ariance Test 1 2 .7 0 9 3 Probability -0 .0 8 0 4 -1 .8 9 5 7 3.6001 2 .1 0 0 8 0 .3 1 3 6 4 .5 1 1 9 0 .0 1 4 6 4 1 0 .0 0 1 9 4 0 Decision(.050) 0 .9 3 5 8 8 0 C annot reject norm ality 0 .0 5 8 0 0 1 C annot reject norm ality 0 .1 6 5 2 9 0 C annot reject norm ality 0 .0 3 5 6 5 4 R eject norm ality 0 .7 5 3 8 2 9 C annot reject norm ality 0 .1 0 4 7 7 4 C annot reject norm ality R eject equal variances R eject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-08 Count 10 12 Median 747 43.115 95.0% LCL of Median 257 9 .1 2 95.0% UCL of Median 1070 3 6 5 .8 4/10/2013 11:17:25 AM 1028 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=D ec-06 R each=R 05, D ate=Jul-08 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 110 165 10 88 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 1 5 .1 6 5 7 5 1 5 .1 6 5 7 5 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.000430 Yes 0.999785 No 0.000215 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 3 .2 9 6 9 0.000978 Yes 3 .2 9 6 9 0.999511 No 3 .2 9 6 9 0.000489 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 3 .2 6 3 9 0.001099 Yes 3 .3 2 9 9 0.999566 No 3 .2 6 3 9 0.000549 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .7 5 0 0 0 0 0 .0 0 0 0 0 0 0 .7 5 0 0 0 0 Reject H0 if Greater Than 0 .5 4 0 8 0 .5 4 0 8 0 .5 4 0 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 0 1 4 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:25 AM 1029 Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R05, Date=Dec Normal Probability Plot of Channel_Catfish_PFGS_ppb when Reach=R05, Date=Jul 1400 n 1200- Channel_Catfish_PFOS_ppb vs Groups Reach=R05, Date=Dec-06 Groups Reach=R05, Date=Jul-08 4/10/2013 11:17:25 AM 1030 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=D ec-06 10 6 9 6 .8 3 8 2 .4 6 8 1 2 0 .9 4 7 4 2 3 .1 9 8 9 9 7 0 .4 0 1 1 R each=R 05, D ate=Jul-09 12 1 8 2 .8 1 8 3 1 7 1 .4 0 4 5 4 9 .4 8 0 2 2 7 3 .9 1 3 0 9 2 9 1 .7 2 3 6 Note: T-alpha (R each =R 05, D ate=D ec-06) = 2 .2622, T-alpha (R each =R 05, D ate=Jul-09) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 20 513.9817 2 8 6 .3 3 1 1 Unequal 11.99 5 1 3.9817 4 1 9 .1 1 9 6 Note: T-alpha (Equal) = 2 .0 8 6 0 , T-alpha (U nequal) = 2 .1 7 9 0 Standard Error 1 2 2 .5 9 9 7 1 3 0 .6 7 7 95.0% LCL Difference 2 5 8 .2 4 3 3 2 2 9 .2 3 4 6 95.0% UCL Difference 7 6 9 .7 2 0 1 7 9 8 .7 2 8 8 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 4 .1 9 2 4 0 .0 0 0 4 4 9 Yes D ifference < 0 4 .1 9 2 4 0 .9 9 9 7 7 6 No D ifference > 0 4 .1 9 2 4 0 .0 0 0 2 2 4 Yes D ifference: (R e a c h = R 0 5 , D ate= D e c -0 6 )-(R e a c h =R 05, Date= Jul-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 7 8 5 0 1 0 .0 0 0 0 0 0 0 .9 9 1 8 0 5 Power (Alpha=.010) 0 .8 9 6 3 2 9 0 .0 0 0 0 0 0 0 .9 4 2 2 5 8 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 3 .9 3 3 2 0 .0 0 1 9 9 0 Yes D ifference < 0 3 .9 3 3 2 0 .9 9 9 0 0 5 No D ifference > 0 3 .9 3 3 2 0 .0 0 0 9 9 5 Yes Difference: (R e a c h = R 05 , D a te = D e c -0 6 )-(R e a c h = R 0 5 , D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 4 9 7 7 8 0 .0 0 0 0 0 0 0 .9 8 0 0 0 0 Power (Alpha=.010) 0 .7 8 8 9 4 3 0 .0 0 0 0 0 0 0 .8 7 4 5 4 5 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-09) Variance-R atio E qual-V ariance Test 4 .9 7 9 0 M odified-Levene E qual-V ariance Test 1 3 .5 6 4 0 Probability -0 .0 8 0 4 -1 .8 9 5 7 3.6001 0 .8 2 6 4 -1.2111 2 .1 4 9 8 0 .0 1 5 1 7 6 0 .0 0 1 4 7 5 Decision(.050) 0 .9 3 5 8 8 0 C annot reject norm ality 0 .0 5 8 0 0 1 C annot reject norm ality 0 .1 6 5 2 9 0 C annot reject norm ality 0 .4 0 8 5 8 7 C annot reject norm ality 0 .2 2 5 8 4 4 C annot reject norm ality 0 .3 4 1 3 3 9 C annot reject norm ality R eject equal variances R eject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Jul-09 Count 10 12 Median 747 136.38 95.0% LCL of Median 257 1 3 .8 7 95.0% UCL of Median 1070 3 4 2 .2 1 4/10/2013 11:17:25 AM 1031 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=D ec-06 R each=R 05, D ate=Jul-09 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 107 162 13 91 Multiplicity Factor = 0 Mean of W 115 138 Std Dev of W 1 5 .1 6 5 7 5 1 5 .1 6 5 7 5 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.001129 Yes 0.999436 No 0.000564 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 3 .0 9 9 1 0.001941 Y es 3 .0 9 9 1 0.999029 No 3 .0 9 9 1 0.000971 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 3 .0 6 6 1 0.002169 Yes 3 .1 3 2 1 0.999132 No 3 .0 6 6 1 0.001084 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 6 6 6 6 7 0 .0 0 0 0 0 0 0 .6 6 6 6 6 7 Reject H0 if Greater Than 0 .5 4 0 8 0 .5 4 0 8 0 .5 4 0 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 0 6 7 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:25 AM 1032 Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R05, Date=Dec Normal Probability Plot of Channel_Catfish_PFGS_ppb when Reach=R05, Date=Jul 1400 n 1200- Channel_Catfish_PFOS_ppb vs Groups Reach=R05, Date=Dec-06 Groups Reach=R05, Date=Jul-09 4/10/2013 11:17:26 AM 1033 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=D ec-06 10 6 9 6 .8 3 8 2 .4 6 8 1 2 0 .9 4 7 4 2 3 .1 9 8 9 9 7 0 .4 0 1 1 R each=R 05, D ate=N ov-12 5 256 1 8 8 .5 8 1 5 8 4 .3 3 6 2 2 1 .8 4 5 1 5 4 9 0 .1 5 4 8 Note: T-alpha (R each =R 05, D ate=D ec-06) = 2 .2622, T-alpha (R each =R 05, D ate=N o v-12) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 440.8 3 3 4 .9 8 4 2 Unequal 12.98 440.8 4 2 6 .4 3 2 6 Note: T-alpha (Equal) = 2 .1 6 0 4 , T-alpha (U nequal) = 2 .1 6 0 8 Standard Error 1 8 3 .4 7 8 4 1 4 7 .4 4 7 5 95.0% LCL Difference 4 4 .4 1 8 9 9 1 2 2 .2 0 1 4 95.0% UCL Difference 837.181 7 5 9 .3 9 8 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .4 0 2 5 0 .0 3 1 9 3 7 Yes D ifference < 0 2 .4 0 2 5 0 .9 8 4 0 3 1 No D ifference > 0 2 .4 0 2 5 0 .0 1 5 9 6 9 Yes II ZJ o en D CD iClD Difference: (R e a c h = R 05 , D ate= D e c -06)-(R each :N ov-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .6 0 3 7 4 1 0 .0 0 0 0 4 2 0 .7 3 5 5 8 5 Power (Alpha=.010) 0 .3 1 7 9 4 2 0 .0 0 0 0 0 3 0 .4 3 1 5 8 5 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .9 8 9 5 0 .0 1 0 4 6 5 Yes D ifference < 0 2 .9 8 9 5 0 .9 9 4 7 6 8 No D ifference > 0 2 .9 8 9 5 0 .0 0 5 2 3 2 Yes Difference: (R e a c h = R 05 , D a te = D e c -0 6 )-(R e a c h = R 0 5 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .7 8 8 9 6 5 0 .0 0 0 0 0 4 0 .8 8 1 6 9 2 Power (Alpha=.010) 0 .5 1 3 4 0 6 0 .0 0 0 0 0 0 0 .6 3 6 3 8 3 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 4 .1 1 3 3 M odified-Levene E qual-V ariance Test 7 .0 8 1 2 Probability -0 .0 8 0 4 -1 .8 9 5 7 3.6001 0 .0 0 0 0 Decision(.050) 0 .9 3 5 8 8 0 C annot reject norm ality 0 .0 5 8 0 0 1 C annot reject norm ality 0 .1 6 5 2 9 0 C annot reject norm ality 1 .0 0 0 0 0 0 C annot reject norm ality 0 .1 8 6 5 0 4 0 .0 1 9 5 9 6 C annot reject equal variances R eject equal variances Median Statistics Variable Reach=R05, Date=Dec-06 Reach=R05, Date=Nov-12 Count 10 5 Median 747 283 95.0% LCL of Median 257 95.0% UCL of Median 1070 4/10/2013 11:17:26 AM 1034 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=D ec-06 R each=R 05, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 40 95 10 25 Multiplicity F acto r = 0 Mean of W 80 40 Std Dev of W 8 .1 6 4 9 6 6 8 .1 6 4 9 6 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.075258 No 0.962371 No 0.037629 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.8371 0.066193 No -1.8371 0.966904 No -1.8371 0.033096 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1 .7 7 5 9 0.075753 No -1 .8 9 8 4 0.971175 No -1 .7 7 5 9 0.037876 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 0 0 0 0 0 0 .0 0 0 0 0 0 0 .6 0 0 0 0 0 Reject H0 if Greater Than 0 .6 6 9 8 0 .6 6 9 8 0 .6 6 9 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .1 6 5 8 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:26 AM Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R05, Date=Dec 1035 1400 n 1200- Channel_Catfish_PFOS_ppb vs Groups 0J---------------- 1--------------------------------- i---------- Reach=R05, Date=Dec-06 Reach=R05, Date=Nov-12 Groups 4/10/2013 11:17:37 AM 1099 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=Jul-07 12 1 2 1 .9 5 8 3 2 1 4 .5 9 3 6 1 .9 4 7 6 7 -1 4 .3 8 7 5 7 2 5 8 .3 0 4 2 R each=R 05, D ate=Jul-08 12 1 2 2 .0 6 4 2 1 7 0 .6 1 0 7 49.2 5 1 0 8 1 3 .6 6 3 2 8 2 3 0 .4 6 5 1 Note: T-alp h a (R each = R 05, D ate=Jul-07) = 2 .2 0 1 0 , T-alpha (R each = R 05, D ate=Ju l-08) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 22 -0 .1 0 5 8 3 3 3 1 9 3 .8 5 3 3 Unequal 20.94 -0 .1058333 2 7 4 .1 4 9 9 Note: T-alp h a (Equal) = 2 .0 7 3 9 , T-alpha (U nequal) = 2 .0 8 0 0 Standard Error 7 9 .1 4 0 2 7 7 9 .1 4 0 2 7 95.0% LCL Difference -1 6 4 .2 3 2 7 -1 6 4 .7 1 7 6 95.0% UCL Difference 164.021 1 6 4 .5 0 5 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .0 0 1 3 0 .9 9 8 9 4 5 No D ifference < 0 -0 .0 0 1 3 0 .4 9 9 4 7 3 No D ifference > 0 -0 .0 0 1 3 0 .5 0 0 5 2 7 No Difference: (R e a c h = R 05 , D ate= Ju l-07)-(R each = R 05, D ate=Ju l-08) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 5 0 0 0 0 0 .0 5 0 1 3 4 0 .0 4 9 8 6 6 Power (Alpha=.010) 0 .0 1 0 0 0 0 0 .0 1 0 0 3 4 0 .0 0 9 9 6 7 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .0 0 1 3 0 .9 9 8 9 4 6 No D ifference < 0 -0 .0 0 1 3 0 .4 9 9 4 7 3 No D ifference > 0 -0 .0 0 1 3 0 .5 0 0 5 2 7 No Difference: (R e a c h = R 05 , D ate = J u l-0 7 )-(R e a c h = R 0 5 , D ate=Ju l-08) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 5 0 0 0 0 0 .0 5 0 1 3 4 0 .0 4 9 8 6 7 Power (Alpha=.010) 0 .0 1 0 0 0 0 0 .0 1 0 0 3 3 0 .0 0 9 9 6 7 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-08) Variance-R atio E qual-V ariance Test 1 .5 8 2 0 M odified-Levene E qual-V ariance Test 0 .0 1 2 3 Probability 2 .7 6 6 2 1 .8 9 3 4 1 1 .2 3 6 8 2 .1 0 0 8 0 .3 1 3 6 4 .5 1 1 9 0 .4 5 9 0 0 8 0 .9 1 2 7 1 2 Decision(.050) 0 .0 0 5 6 7 2 R eject norm ality 0 .0 5 8 3 0 5 C annot reject norm ality 0 .0 0 3 6 3 1 R eject norm ality 0 .0 3 5 6 5 4 R eject norm ality 0 .7 5 3 8 2 9 C annot reject norm ality 0 .1 0 4 7 7 4 C annot reject norm ality C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Jul-08 Count 12 12 Median 8.65 43.115 95.0% LCL of Median 7 .3 9 .1 2 95.0% UCL of Median 302.1 3 6 5 .8 4/10/2013 11:17:37 AM 1100 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=Jul-07 R each= R 05, D ate=Jul-08 N u m b e r S ets o f T ie s = 1, Mann W Whitney U Sum Ranks 50 128 94 172 Multiplicity Factor = 6 Mean of W 150 150 Std Dev of W 1 7 .3 1 6 7 4 1 7 .3 1 6 7 4 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1 .2 7 0 4 0.203926 No -1 .2 7 0 4 0.101963 No -1 .2 7 0 4 0.898037 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1 .2 4 1 6 0.214394 No -1 .2 4 1 6 0.107197 No -1 .2 9 9 3 0.903083 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 1 6 6 6 7 0 .4 1 6 6 6 7 0 .0 8 3 3 3 3 Reject H0 if Greater Than 0 .5 1 7 9 0 .5 1 7 9 0 .5 1 7 9 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .2 5 5 8 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:37 AM 1101 4/10/2013 11:17:38 AM 1102 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=Jul-07 12 1 2 1 .9 5 8 3 2 1 4 .5 9 3 6 1 .9 4 7 6 7 -1 4 .3 8 7 5 7 2 5 8 .3 0 4 2 R each=R 05, D ate=Jul-09 12 1 8 2 .8 1 8 3 1 7 1 .4 0 4 5 49.4 8 0 2 2 7 3 .9 1 3 0 9 2 9 1 .7 2 3 6 Note: T-alp h a (R each = R 05, D ate=Jul-07) = 2 .2 0 1 0 , T-alpha (R each = R 05, D ate=Ju l-09) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 22 -60.86 1 9 4 .2 0 3 1 Unequal 20.98 -60.86 2 7 4 .6 4 4 7 Note: T-alp h a (Equal) = 2 .0 7 3 9 , T-alpha (U nequal) = 2 .0 7 9 8 Standard Error 7 9 .2 8 3 0 8 7 9 .2 8 3 0 8 95.0% LCL Difference -2 2 5 .2 8 3 -2 2 5 .7 4 9 9 95.0% UCL Difference 1 0 3 .5 6 3 1 0 4 .0 2 9 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .7 6 7 6 0 .4 5 0 8 6 8 No D ifference < 0 -0 .7 6 7 6 0 .2 2 5 4 3 4 No D ifference > 0 -0 .7 6 7 6 0 .7 7 4 5 6 6 No Difference: (R e a c h = R 05 , D ate= Ju l-07)-(R each = R 05, D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 1 3 7 3 9 0 .1 8 3 8 7 6 0 .0 0 8 4 3 7 Power (Alpha=.010) 0 .0 3 1 6 7 1 0 .0 5 4 2 8 9 0 .0 0 1 1 4 7 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .7 6 7 6 0 .4 5 1 2 6 3 No D ifference < 0 -0 .7 6 7 6 0 .2 2 5 6 3 1 No D ifference > 0 -0 .7 6 7 6 0 .7 7 4 3 6 9 No Difference: (R e a c h = R 05 , D ate = J u l-0 7 )-(R e a c h = R 0 5 , D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 1 3 4 5 6 0 .1 8 3 5 7 4 0 .0 0 8 4 6 2 Power (Alpha=.010) 0 .0 3 1 4 9 3 0 .0 5 4 0 5 1 0 .0 0 1 1 5 5 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-09) Variance-R atio E qual-V ariance Test 1 .5 6 7 4 M odified-Levene E qual-V ariance Test 0 .1 2 4 8 Probability 2 .7 6 6 2 1 .8 9 3 4 1 1 .2 3 6 8 0 .8 2 6 4 -1.2111 2 .1 4 9 8 0 .4 6 8 0 7 4 0 .7 2 7 2 2 6 Decision(.050) 0 .0 0 5 6 7 2 R eject norm ality 0 .0 5 8 3 0 5 C annot reject norm ality 0 .0 0 3 6 3 1 R eject norm ality 0 .4 0 8 5 8 7 C annot reject norm ality 0 .2 2 5 8 4 4 C annot reject norm ality 0 .3 4 1 3 3 9 C annot reject norm ality C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Jul-09 Count 12 12 Median 8.65 136.38 95.0% LCL of Median 7 .3 1 3 .8 7 95.0% UCL of Median 302.1 3 4 2 .2 1 4/10/2013 11:17:38 AM 1103 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=Jul-07 R each=R 05, D ate=Jul-09 N u m b e r S ets o f T ie s = 1, Mann W Whitney U Sum Ranks 52 130 92 170 M u ltip licity F a c to r= 6 Mean of W 150 150 Std Dev of W 1 7 .3 1 6 7 4 1 7 .3 1 6 7 4 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1 .1 5 5 0 0.248110 No -1 .1 5 5 0 0.124055 No -1 .1 5 5 0 0.875945 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.1261 0.260133 No -1.1261 0.130066 No -1 .1 8 3 8 0.881759 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 1 6 6 6 7 0 .4 1 6 6 6 7 0 .1 6 6 6 6 7 Reject H0 if Greater Than 0 .5 1 7 9 0 .5 1 7 9 0 .5 1 7 9 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .2 5 5 8 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:38 AM 1104 4/10/2013 11:17:38 AM 1105 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=Jul-07 12 1 2 1 .9 5 8 3 2 1 4 .5 9 3 6 1 .9 4 7 6 7 -1 4 .3 8 7 5 7 2 5 8 .3 0 4 2 R each=R 05, D ate=N ov-12 5 256 1 8 8 .5 8 1 5 8 4 .3 3 6 2 2 1 .8 4 5 1 5 4 9 0 .1 5 4 8 Note: T-alpha (R each =R 05, D ate=Jul-07) = 2 .2010, T-alpha (R each =R 05, D ate=N o v-12) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 15 -134.0417 2 0 7 .9 7 5 Unequal 8.57 -1 3 4 .0 4 1 7 2 8 5 .6 8 0 1 Note: T-alpha (Equal) = 2 .1 3 1 4 , T-alpha (U nequal) = 2 .2 7 9 4 Standard Error 1 1 0 .7 0 3 1 1 0 4 .6 4 2 8 95.0% LCL Difference -3 6 9 .9 9 9 8 -3 7 2 .5 6 8 7 95.0% UCL Difference 1 0 1 .9 1 6 5 1 0 4 .4 8 5 4 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1 .2 1 0 8 0 .2 4 4 6 9 7 No D ifference < 0 -1 .2 1 0 8 0 .1 2 2 3 4 9 No D ifference > 0 -1 .2 1 0 8 0 .8 7 7 6 5 1 No Difference: (R e a c h = R 05 , D ate= Ju l-07)-(R each = R 05, D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 0 5 4 8 6 0 .3 1 2 5 7 3 0 .0 0 2 5 2 8 Power (Alpha=.010) 0 .0 6 7 8 6 8 0 .1 1 0 9 8 6 0 .0 0 0 2 9 4 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1 .2 8 0 9 0 .2 3 3 7 7 6 No D ifference < 0 -1 .2 8 0 9 0 .1 1 6 8 8 8 No D ifference > 0 -1 .2 8 0 9 0 .8 8 3 1 1 2 No Difference: (R e a c h = R 05 , D ate = J u l-0 7 )-(R e a c h = R 0 5 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 0 7 3 6 3 0 .3 2 1 0 8 4 0 .0 0 2 3 0 7 Power (Alpha=.010) 0 .0 6 4 0 9 0 0 .1 0 8 2 4 0 0 .0 0 0 2 9 6 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1 .2 9 4 9 M odified-Levene E qual-V ariance Test 0 .0 2 7 5 Probability 2 .7 6 6 2 1 .8 9 3 4 1 1 .2 3 6 8 0 .0 0 0 0 Decision(.050) 0 .0 0 5 6 7 2 R eject norm ality 0 .0 5 8 3 0 5 C annot reject norm ality 0 .0 0 3 6 3 1 R eject norm ality 1 .0 0 0 0 0 0 C annot reject norm ality 0 .8 6 9 1 0 7 0 .8 7 0 4 3 4 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-07 Reach=R05, Date=Nov-12 Count 12 5 Median 8.65 283 95.0% LCL of Median 7 .3 95.0% UCL of Median 302.1 4/10/2013 11:17:39 AM 1106 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=Jul-07 R each=R 05, D ate=N ov-12 N um ber S ets of Ties = 1, Mann W Whitney U Sum Ranks 13 91 47 62 Multiplicity F acto r = 6 Mean of W 108 45 Std Dev of W 9 .4 8 1 0 1 8 9 .4 8 1 0 1 8 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.7931 0.072964 No 1.7931 0.036482 Yes 1.7931 0.963518 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1 .7 4 0 3 0.081803 No 1 .7 4 0 3 0.040901 Y es 1 .8 4 5 8 0.967539 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .7 5 0 0 0 0 0 .7 5 0 0 0 0 0 .0 8 3 3 3 3 Reject H0 if Greater Than 0 .6 5 4 4 0 .6 5 4 4 0 .6 5 4 4 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 2 1 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:39 AM 1107 4/10/2013 11:17:50 AM 1171 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=Jul-08 12 1 2 2 .0 6 4 2 1 7 0 .6 1 0 7 49.2 5 1 0 8 1 3 .6 6 3 2 8 2 3 0 .4 6 5 1 R each=R 05, D ate=Jul-09 12 1 8 2 .8 1 8 3 1 7 1 .4 0 4 5 49.4 8 0 2 2 7 3 .9 1 3 0 9 2 9 1 .7 2 3 6 Note: T-alp h a (R each = R 05, D ate=Jul-08) = 2 .2 0 1 0 , T-alpha (R each = R 05, D ate=Ju l-09) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 22 -6 0 .7 5 4 1 7 1 7 1 .0 0 8 1 Unequal 22.00 -6 0 .7 5 4 1 7 2 4 1 .8 4 1 9 Note: T-alp h a (Equal) = 2 .0 7 3 9 , T-alpha (U nequal) = 2 .0 7 3 9 Standard Error 69.8 1 3 7 6 69.8 1 3 7 6 95.0% LCL Difference -2 0 5 .5 3 9 -2 0 5 .5 3 9 2 95.0% UCL Difference 8 4 .0 3 0 7 1 8 4 .0 3 0 8 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .8 7 0 2 0 .3 9 3 5 7 0 No D ifference < 0 -0 .8 7 0 2 0 .1 9 6 7 8 5 No D ifference > 0 -0 .8 7 0 2 0 .8 0 3 2 1 5 No Difference: (R e a c h = R 05 , D ate= Ju l-08)-(R each = R 05, D ate=Ju l-09) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 3 2 4 3 2 0 .2 1 1 4 9 1 0 .0 0 6 4 0 5 Power (Alpha=.010) 0 .0 3 8 8 1 6 0 .0 6 5 7 4 7 0 .0 0 0 8 2 8 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .8 7 0 2 0 .3 9 3 5 7 0 No D ifference < 0 -0 .8 7 0 2 0 .1 9 6 7 8 5 No D ifference > 0 -0 .8 7 0 2 0 .8 0 3 2 1 5 No Difference: (R e a c h = R 05 , D ate = J u l-0 8 )-(R e a c h = R 0 5 , D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 3 2 4 3 2 0 .2 1 1 4 9 1 0 .0 0 6 4 0 5 Power (Alpha=.010) 0 .0 3 8 8 1 6 0 .0 6 5 7 4 7 0 .0 0 0 8 2 8 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-08) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-09) Variance-R atio E qual-V ariance Test 1 .0 0 9 3 M odified-Levene E qual-V ariance Test 0 .3 7 3 2 Probability 2 .1 0 0 8 0 .3 1 3 6 4 .5 1 1 9 0 .8 2 6 4 -1.2111 2 .1 4 9 8 0 .9 8 7 9 9 2 0 .5 4 7 5 2 9 Decision(.050) 0 .0 3 5 6 5 4 R eject norm ality 0 .7 5 3 8 2 9 C annot reject norm ality 0 .1 0 4 7 7 4 C annot reject norm ality 0 .4 0 8 5 8 7 C annot reject norm ality 0 .2 2 5 8 4 4 C annot reject norm ality 0 .3 4 1 3 3 9 C annot reject norm ality C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-08 Reach=R05, Date=Jul-09 Count 12 12 Median 43.115 136.38 95.0% LCL of Median 9 .1 2 1 3 .8 7 95.0% UCL of Median 3 6 5 .8 3 4 2 .2 1 4/10/2013 11:17:50 AM 1172 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=Jul-08 R each= R 05, D ate=Jul-09 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 59 137 85 163 Multiplicity F acto r = 0 Mean of W 150 150 Std Dev of W 1 7 .3 2 0 5 1 1 7 .3 2 0 5 1 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.477575 No 0.238788 No 0.761212 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0 .7 5 0 6 0.45 2 9 2 0 No -0 .7 5 0 6 0.22 6 4 6 0 No -0 .7 5 0 6 0.77 3 5 4 0 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0 .7 2 1 7 0.470486 No -0 .7 2 1 7 0.235243 No -0 .7 7 9 4 0.782135 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 1 6 6 6 7 0 .4 1 6 6 6 7 0 .1 6 6 6 6 7 Reject H0 if Greater Than 0 .5 1 7 9 0 .5 1 7 9 0 .5 1 7 9 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .2 5 5 8 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:51 AM 1173 500 n 400 - Channel_Catfish_F'FOS_ppb vs Groups R'each=R05, Date-Jul-08 Reach=R05, Date=Jul-09 Groups 4/10/2013 11:17:51 AM 1174 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each= R 05, D ate=Jul-08 12 1 2 2 .0 6 4 2 1 7 0 .6 1 0 7 49.2 5 1 0 8 1 3 .6 6 3 2 8 2 3 0 .4 6 5 1 R each=R 05, D ate=N ov-12 5 256 1 8 8 .5 8 1 5 8 4 .3 3 6 2 21.8 4 5 1 5 4 9 0 .1 5 4 8 Note: T-alp h a (R each = R 05, D ate=Jul-08) = 2 .2 0 1 0 , T-alpha (R each = R 05, D ate =N o v-12) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 15 -133.9358 1 7 5 .5 8 2 9 Unequal 6.90 -1 3 3 .9 3 5 8 2 5 4 .3 0 4 9 Note: T-alp h a (Equal) = 2 .1 3 1 4 , T-alpha (U nequal) = 2 .3 7 1 5 Standard Error 9 3 .4 6 1 1 1 9 7 .6 6 4 0 4 95.0% LCL Difference -3 3 3 .1 4 3 5 -3 6 5 .5 4 3 5 95.0% UCL Difference 65.2 7 1 8 2 9 7 .6 7 1 7 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1.4331 0 .1 7 2 3 5 2 No D ifference < 0 -1.4331 0 .0 8 6 1 7 6 No D ifference > 0 -1.4331 0 .9 1 3 8 2 4 No Difference: (R e a c h = R 05 , D ate= Ju l-08)-(R each = R 05, D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 6 8 8 1 9 0 .3 9 1 0 4 7 0 .0 0 1 2 7 9 Power (Alpha=.010) 0 .0 9 7 7 3 9 0 .1 5 4 0 7 1 0 .0 0 0 1 3 5 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1 .3 7 1 4 0 .2 1 3 1 7 4 No D ifference < 0 -1 .3 7 1 4 0 .1 0 6 5 8 7 No D ifference > 0 -1 .3 7 1 4 0 .8 9 3 4 1 3 No Difference: (R e a c h = R 05 , D ate = J u l-0 8 )-(R e a c h = R 0 5 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 2 0 3 8 5 0 .3 4 1 8 1 4 0 .0 0 1 8 9 2 Power (Alpha=.010) 0 .0 6 6 8 3 4 0 .1 1 4 1 4 6 0 .0 0 0 2 5 2 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-08) Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1 .2 2 1 8 M odified-Levene E qual-V ariance Test 0 .1 0 8 2 Probability 2 .1 0 0 8 0 .3 1 3 6 4 .5 1 1 9 0 .0 0 0 0 Decision(.050) 0 .0 3 5 6 5 4 R eject norm ality 0 .7 5 3 8 2 9 C annot reject norm ality 0 .1 0 4 7 7 4 C annot reject norm ality 1 .0 0 0 0 0 0 C annot reject norm ality 0 .7 1 2 8 6 5 0 .7 4 6 7 0 3 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-08 Reach=R05, Date=Nov-12 Count 12 5 Median 43.115 283 95.0% LCL of Median 9 .1 2 95.0% UCL of Median 3 6 5 .8 4/10/2013 11:17:51 AM 1175 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=Jul-08 R each=R 05, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 12 90 48 63 Multiplicity F acto r = 0 Mean of W 108 45 Std Dev of W 9 .4 8 6 8 3 3 9 .4 8 6 8 3 3 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.063672 No 0.031836 Yes 0.968164 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1 .8 9 7 4 0.057780 No 1 .8 9 7 4 0.028890 Yes 1 .8 9 7 4 0.971110 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1 .8 4 4 7 0.065087 No 1 .8 4 4 7 0.032543 Yes 1.9501 0.974416 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .7 5 0 0 0 0 0 .7 5 0 0 0 0 0 .0 5 0 0 0 0 Reject H0 if Greater Than 0 .6 5 4 4 0 .6 5 4 4 0 .6 5 4 4 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 2 1 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:51 AM 1176 550 n 440 - Channel_Catfish_F'FOS_ppb vs Groups Reach=R05, Date=Jul-08 Reach=R05, Da1e=Nov-12 Groups 4/10/2013 11:18:03 AM 1240 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=Jul-09 12 1 8 2 .8 1 8 3 1 7 1 .4 0 4 5 49.4 8 0 2 2 7 3 .9 1 3 0 9 2 9 1 .7 2 3 6 R each=R 05, D ate=N ov-12 5 256 1 8 8 .5 8 1 5 8 4 .3 3 6 2 21.8 4 5 1 5 4 9 0 .1 5 4 8 Note: T-alp h a (R each = R 05, D ate=Jul-09) = 2 .2 0 1 0 , T-alpha (R each = R 05, D ate=N o v-12) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 15 -73.18166 1 7 6 .1 4 8 9 Unequal 6.93 -7 3 .1 8 1 6 6 2 5 4 .8 3 8 2 Note: T-alp h a (Equal) = 2 .1 3 1 4 , T-alpha (U nequal) = 2 .3 6 9 5 Standard Error 9 3 .7 6 2 4 1 9 7 .7 7 9 7 9 95.0% LCL Difference -2 7 3 .0 3 1 5 -3 0 4 .8 7 4 2 95.0% UCL Difference 1 2 6 .6 6 8 2 1 5 8 .5 1 0 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .7 8 0 5 0 .4 4 7 2 3 9 No D ifference < 0 -0 .7 8 0 5 0 .2 2 3 6 1 9 No D ifference > 0 -0 .7 8 0 5 0 .7 7 6 3 8 1 No Difference: (R e a c h = R 05 , D ate= Ju l-09)-(R each = R 05, D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 1 3 2 0 3 0 .1 8 4 2 6 3 0 .0 0 8 3 9 1 Power (Alpha=.010) 0 .0 3 0 7 9 5 0 .0 5 3 3 3 6 0 .0 0 1 1 7 6 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .7 4 8 4 0 .4 7 8 8 2 7 No D ifference < 0 -0 .7 4 8 4 0 .2 3 9 4 1 4 No D ifference > 0 -0 .7 4 8 4 0 .7 6 0 5 8 6 No Difference: (R e a c h = R 05 , D ate = J u l-0 9 )-(R e a c h = R 0 5 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 9 9 6 2 7 0 .1 6 6 6 1 7 0 .0 1 0 0 0 7 Power (Alpha=.010) 0 .0 2 4 3 8 1 0 .0 4 3 6 4 1 0 .0 0 1 5 7 8 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-09) Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1 .2 1 0 5 M odified-Levene E qual-V ariance Test 0 .0 1 5 2 Probability 0 .8 2 6 4 -1.2111 2 .1 4 9 8 0 .0 0 0 0 Decision(.050) 0 .4 0 8 5 8 7 C annot reject norm ality 0 .2 2 5 8 4 4 C annot reject norm ality 0 .3 4 1 3 3 9 C annot reject norm ality 1 .0 0 0 0 0 0 C annot reject norm ality 0 .7 2 1 1 9 3 0 .9 0 3 4 2 8 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-09 Reach=R05, Date=Nov-12 Count 12 5 Median 136.38 283 95.0% LCL of Median 1 3 .8 7 95.0% UCL of Median 3 4 2 .2 1 4/10/2013 11:18:03 AM 1241 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=Jul-09 R each=R 05, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 102 36 51 Multiplicity F acto r = 0 Mean of W 108 45 Std Dev of W 9 .4 8 6 8 3 3 9 .4 8 6 8 3 3 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.574337 No 0.287169 No 0.712831 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0 .6 3 2 5 0.527089 No 0 .6 3 2 5 0.263545 No 0 .6 3 2 5 0.736455 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0 .5 7 9 8 0.562083 No 0 .5 7 9 8 0.281041 No 0 .6 8 5 2 0.753379 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .3 3 3 3 3 3 0 .3 3 3 3 3 3 0 .1 3 3 3 3 3 Reject H0 if Greater Than 0 .6 5 4 4 0 .6 5 4 4 0 .6 5 4 4 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0.7251 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:03 AM Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R05, Date=Jul 1242 550 n 440 - Channel_Catfish_PFOS_ppb vs Groups Reach=R05, Date=Jul-09 Reach=R05, Date-Nov-12 Groups 4/10/2013 11:18:26 AM 1369 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 06, Date=Jun-02 7 2.5 0 0 2.5 2.5 R each=R 06, D ate=N ov-04 5 3 .4 0 8 1 .1 7 3 6 1 4 0 .5 2 4 8 5 6 2 1 .9 5 0 7 6 6 4 .8 6 5 2 3 4 Note: T-alpha (R each =R 06, D ate=Jun-02) = 0.0000, T-alpha (R each =R 06, D ate=N o v-04) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -0.908 0 .7 4 2 2 5 8 7 Unequal 4.00 -0.908 1 .1 7 3 6 1 4 Note: T-alp h a (Equal) = 2 .2 2 8 1 , T-alpha (U nequal) = 2 .7 7 6 4 Standard Error 0 .4 3 4 6 2 2 2 0 .5 2 4 8 5 6 2 95.0% LCL Difference -1 .8 7 6 3 9 9 -2 .3 6 5 2 3 4 95.0% UCL Difference 0 .0 6 0 3 9 8 6 1 0 .5 4 9 2 3 4 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .0 8 9 2 0 .0 6 3 2 1 7 No D ifference < 0 -2 .0 8 9 2 0 .0 3 1 6 0 9 Yes D ifference > 0 -2 .0 8 9 2 0 .9 6 8 3 9 1 No Difference: (R e a c h = R 06 , D ate=Ju n -02)-(R each =:R 06, D ate=N o v-04) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 7 1 3 4 6 0 .6 1 8 0 0 9 0 .0 0 0 1 5 6 Power (Alpha=.010) 0 .2 0 6 5 2 3 0 .3 0 3 7 6 1 0 .0 0 0 0 1 5 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 -1 .7 3 0 0 0 .1 5 8 6 8 3 No D ifference < 0 -1 .7 3 0 0 0 .0 7 9 3 4 1 No D ifference > 0 -1 .7 3 0 0 0 .9 2 0 6 5 9 No Difference: (R e a c h = R 06 , D a te = J u n -0 2 )-(R e a c h = R 0 6 , D ate=N o v-04) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 6 6 4 3 5 0 .4 1 7 5 9 3 0 .0 0 0 8 4 5 Power (Alpha=.010) 0 .0 7 4 7 9 2 0 .1 3 3 1 1 7 0 .0 0 0 1 2 8 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 06 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 6 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 06 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 06 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 06 , D ate= N o v-04) Variance-R atio E qual-V ariance Test 0 .0 0 0 0 M odified-Levene E qual-V ariance Test 1 3 .7 7 0 4 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 0 0 0 .0 0 4 0 3 6 R eject equal variances R eject equal variances Median Statistics Variable Reach=R06, Date=Jun-02 Reach=R06, Date=Nov-04 Count 7 5 Median 2.5 3.16 95.0% LCL of Median 2 .5 95.0% UCL of Median 2 .5 4/10/2013 11:18:26 AM 1370 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 06, Date=Jun-02 R each=R 06, D ate=N ov-04 N um ber S ets of Ties = 1, Mann W Whitney U Sum Ranks 7 35 28 43 Multiplicity F acto r = 3 3 6 Mean of W 4 5 .5 3 2 .5 Std Dev of W 5 .5 2 1 9 9 5 5 .5 2 1 9 9 5 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1 .9 0 1 5 0.057238 No 1 .9 0 1 5 0.028619 Yes 1 .9 0 1 5 0.971381 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1 .8 1 0 9 0.070150 No 1 .8 1 0 9 0.035075 Yes 1 .9 9 2 0 0.976816 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .8 0 0 0 0 0 0 .8 0 0 0 0 0 0 .2 0 0 0 0 0 Reject H0 if Greater Than 0.7061 0 .7 0 6 1 0 .7 0 6 1 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 3 0 3 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:27 AM 1371 4/10/2013 11:18:27 AM 1372 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 06, Date=Jun-02 7 2.5 0 0 2.5 2.5 R each=R 06, D ate=D ec-06 5 6 .0 2 5 .4 6 9 3 2 8 2 .4 4 5 9 5 8 -0 .7 7 1 0 6 7 8 1 2 .8 1 1 0 7 Note: T-alpha (R each =R 06, D ate=Jun-02) = 0.0000, T-alpha (R each =R 06, D ate=D ec-06) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -3.52 3 .4 5 9 1 0 7 Unequal 4.00 -3.52 5 .4 6 9 3 2 8 Note: T-alp h a (Equal) = 2 .2 2 8 1 , T-alpha (U nequal) = 2 .7 7 6 4 Standard Error 2.02 5 4 4 5 2.44 5 9 5 8 95.0% LCL Difference -8 .0 3 2 9 7 4 -1 0 .3 1 1 0 7 95.0% UCL Difference 0 .9 9 2 9 7 3 9 3 .2 7 1 0 6 8 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1 .7 3 7 9 0 .1 1 2 8 6 9 No D ifference < 0 -1 .7 3 7 9 0 .0 5 6 4 3 4 No D ifference > 0 -1 .7 3 7 9 0 .9 4 3 5 6 6 No Difference: (R e a c h = R 06 , D ate=Ju n -02)-(R each =:R 06, Date= D ec-06) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .3 4 9 2 9 2 0 .4 8 9 7 3 8 0 .0 0 0 5 2 9 Power (Alpha=.010) 0 .1 3 3 3 7 4 0 .2 0 7 3 1 3 0 .0 0 0 0 5 6 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 -1.4391 0 .2 2 3 5 2 3 No D ifference < 0 -1.4391 0 .1 1 1 7 6 1 No D ifference > 0 -1.4391 0 .8 8 8 2 3 9 No Difference: (R e a c h = R 06 , D a te = J u n -0 2 )-(R e a c h = R 0 6 , D a te = D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 0 0 3 8 7 0 .3 2 7 9 7 3 0 .0 0 1 9 6 0 Power (Alpha=.010) 0 .0 5 2 4 5 6 0 .0 9 5 4 4 2 0 .0 0 0 3 0 7 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 06 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 6 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 06 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 06 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 06 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 0 .0 0 0 0 M odified-Levene E qual-V ariance Test 5 .8 9 0 7 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 0 0 0 .0 3 5 6 2 8 R eject equal variances R eject equal variances Median Statistics Variable Reach=R06, Date=Jun-02 Reach=R06, Date=Dec-06 Count 7 5 Median 2.5 4.29 95.0% LCL of Median 2 .5 95.0% UCL of Median 2 .5 4/10/2013 11:18:27 AM 1373 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 06, Date=Jun-02 R each=R 06, D ate=D ec-06 N um ber S ets of Ties = 1, Mann W Whitney U Sum Ranks 7 35 28 43 Multiplicity F acto r = 3 3 6 Mean of W 4 5 .5 3 2 .5 Std Dev of W 5 .5 2 1 9 9 5 5 .5 2 1 9 9 5 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Approx. With Correction Prob Reject H0 Prob Reject H0 Z-Value Level at .050 Z-Value Level at .050 1 .9 0 1 5 0.057238 No 1 .8 1 0 9 0.070150 No 1 .9 0 1 5 0.028619 Y es 1 .8 1 0 9 0.035075 Yes 1 .9 0 1 5 0.971381 No 1 .9 9 2 0 0.976816 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .8 0 0 0 0 0 0 .8 0 0 0 0 0 0 .2 0 0 0 0 0 Reject H0 if Greater Than 0.7061 0 .7 0 6 1 0 .7 0 6 1 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 3 0 3 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:27 AM 1374 4/10/2013 11:18:27 AM 1375 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 06, Date=Jun-02 7 2.5 0 0 2.5 2.5 R each=R 06, D ate=N ov-12 5 3 .7 0 4 0 .9 1 8 5 2 0 6 0 .4 1 0 7 7 4 9 2 .5 6 3 5 0 6 4 .8 4 4 4 9 4 Note: T-alpha (R each =R 06, D ate=Jun-02) = 0.0000, T-alpha (R each =R 06, D ate=N o v-12) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -1.204 0 .5 8 0 9 2 3 4 Unequal 4.00 -1.204 0 .9 1 8 5 2 0 6 Note: T-alp h a (Equal) = 2 .2 2 8 1 , T-alpha (U nequal) = 2 .7 7 6 4 Standard Error 0 .3 4 0 1 5 3 9 0 .4 1 0 7 7 4 9 95.0% LCL Difference -1.96191 -2 .3 4 4 4 9 4 95.0% UCL Difference -0 .4 4 6 0 8 9 8 -0 .0 6 3 5 0 6 1 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -3 .5 3 9 6 0 .0 0 5 3 6 1 Yes D ifference < 0 -3 .5 3 9 6 0 .0 0 2 6 8 0 Yes D ifference > 0 -3 .5 3 9 6 0 .9 9 7 3 2 0 No Difference: (R e a c h = R 06 , D ate=Ju n -02)-(R each =:R 06, D ate=N o v-12) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .8 8 9 6 0 8 0 .9 4 9 6 6 9 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .6 4 5 7 4 9 0 .7 6 5 2 0 2 0 .0 0 0 0 0 0 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 -2 .9 3 1 0 0 .0 4 2 7 6 9 Yes D ifference < 0 -2 .9 3 1 0 0 .0 2 1 3 8 5 Yes D ifference > 0 -2 .9 3 1 0 0 .9 7 8 6 1 5 No Difference: (R e a c h = R 06 , D a te = J u n -0 2 )-(R e a c h = R 0 6 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .6 0 0 3 1 5 0 .7 7 5 0 7 3 0 .0 0 0 0 1 3 Power (Alpha=.010) 0 .2 3 3 3 4 5 0 .3 6 9 6 4 0 0 .0 0 0 0 0 2 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 06 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 6 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 06 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 06 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 06 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 0 .0 0 0 0 M odified-Levene E qual-V ariance Test 4 .6 3 4 4 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 0 0 0 .0 5 6 8 0 0 R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R06, Date=Jun-02 Reach=R06, Date=Nov-12 Count 7 5 Median 2.5 4.15 95.0% LCL of Median 2 .5 95.0% UCL of Median 2 .5 4/10/2013 11:18:28 AM 1376 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 06, Date=Jun-02 R each=R 06, D ate=N ov-12 N um ber Sets of Ties = 2, Mann W Whitney U Sum Ranks 7 35 28 43 Multiplicity F acto r = 3 4 2 Mean of W 4 5 .5 3 2 .5 Std Dev of W 5 .5 0 9 9 7 7 5 .5 0 9 9 7 7 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Approx. With Correction Prob Reject H0 Prob Reject H0 Z-Value Level at .050 Z-Value Level at .050 1 .9 0 5 6 0.056698 No 1 .8 1 4 9 0.069541 No 1 .9 0 5 6 0.028349 Y es 1 .8 1 4 9 0.034770 Yes 1 .9 0 5 6 0.971651 No 1 .9 9 6 4 0.977054 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .8 0 0 0 0 0 0 .8 0 0 0 0 0 0 .2 0 0 0 0 0 Reject H0 if Greater Than 0.7061 0 .7 0 6 1 0 .7 0 6 1 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 3 0 3 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:28 AM 1377 4/10/2013 11:18:37 AM 1429 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 06, D ate=N ov-04 5 3 .4 0 8 1 .1 7 3 6 1 4 0 .5 2 4 8 5 6 2 1 .9 5 0 7 6 6 4 .8 6 5 2 3 4 R each=R 06, D ate=D ec-06 5 6 .0 2 5.46 9 3 2 8 2 .445958 -0 .7 7 1 0 6 7 8 1 2 .8 1 1 0 7 Note: T-alp h a (R each = R 06, D ate=N o v-04) = 2 .7 7 6 4 , T-alpha (R each = R 06, D a te= D ec-06) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -2.612 3 .9 5 5 4 3 4 Unequal 4.37 -2.612 5 .5 9 3 8 2 9 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .6 8 6 7 Standard Error 2.50 1 6 3 6 2.50 1 6 3 6 95.0% LCL Difference -8 .3 8 0 7 8 4 -9 .3 3 3 0 8 3 95.0% UCL Difference 3 .1 5 6 7 8 4 4.10 9 0 8 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1.0441 0 .3 2 6 9 5 2 No D ifference < 0 -1.0441 0 .1 6 3 4 7 6 No D ifference > 0 -1.0441 0 .8 3 6 5 2 4 No D iffe re n c e : (R e a c h = R 0 6 , D a te = N o v -0 4 )-(R e a c h = R 0 6 , D a te := D e c -0 6 ) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 5 1 8 7 0 0 .2 4 5 7 2 3 0 .0 0 4 5 5 9 Power (Alpha=.010) 0 .0 4 2 3 7 6 0 .0 7 4 0 6 1 0 .0 0 0 6 4 0 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1.0441 0 .3 5 0 7 3 9 No D ifference < 0 -1.0441 0 .1 7 5 3 7 0 No D ifference > 0 -1.0441 0 .8 2 4 6 3 0 No D iffe re n c e : (R e a c h = R 0 6 , D a te = N o v -0 4 )-(R e a c h = R 0 6 , D a te := D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 3 2 2 7 8 0 .2 2 4 4 6 9 0 .0 0 5 3 7 4 Power (Alpha=.010) 0 .0 3 2 3 2 7 0 .0 5 9 4 6 0 0 .0 0 0 8 7 1 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 06 , D ate= N ov-04) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 06 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 06 , D ate= D ec-06) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 06 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 2 1 .7 1 7 9 M odified-Levene E qual-V ariance Test 2 .2 3 9 8 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 1 1 2 8 4 0 .1 7 2 8 6 6 R eject equal variances C annot reject equal variances Median Statistics Variable R each=R 06, D ate=N ov-04 R each=R 06, D ate=D ec-06 Count 5 5 Median 3 .1 6 4 .2 9 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:18:37 AM 1430 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 06, D ate=N ov-04 R each=R 06, D ate=D ec-06 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 9 24 16 31 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.273810 No 0.726191 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.7311 0.464702 No -0.7311 0.232351 No -0.7311 0.767649 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0 .6 2 6 7 0.530869 No -0 .6 2 6 7 0.265435 No -0 .8 3 5 6 0.798302 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .4 0 0 0 0 0 0 .2 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .8 7 3 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:38 AM 1431 4/10/2013 11:18:38 AM 1432 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 06, D ate=N ov-04 5 3 .4 0 8 1 .1 7 3 6 1 4 0 .5 2 4 8 5 6 2 1 .9 5 0 7 6 6 4 .8 6 5 2 3 4 R each=R 06, D ate=N ov-12 5 3 .7 0 4 0 .9 1 8 5 2 0 6 0 .4 1 0 7 7 4 9 2.56 3 5 0 6 4 .8 4 4 4 9 4 Note: T-alp h a (R each = R 06, D ate=N o v-04) = 2 .7 7 6 4 , T-alpha (R each = R 06, D ate=N o v-12) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -0.296 1 .0 5 3 8 1 5 Unequal 7.56 -0.296 1 .4 9 0 3 1 9 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .3 2 9 4 Standard Error 0 .6 6 6 4 9 0 8 0 .6 6 6 4 9 0 8 95.0% LCL Difference -1.832931 -1 .8 4 8 5 1 5 95.0% UCL Difference 1 .2 4 0 9 3 1 1 .2 5 6 5 1 5 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0.4441 0 .6 6 8 7 2 6 No D ifference < 0 -0.4441 0 .3 3 4 3 6 3 No D ifference > 0 -0.4441 0 .6 6 5 6 3 7 No Difference: (R e a c h = R 06 , D a te = N o v -0 4 )-(R e a c h = R 0 6 , D ate=N o v-12) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 6 7 9 1 4 0 .1 0 8 0 1 9 0 .0 2 0 0 0 5 Power (Alpha=.010) 0 .0 1 5 0 9 2 0 .0 2 5 5 7 3 0 .0 0 3 4 1 5 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0.4441 0 .6 6 9 3 8 9 No D ifference < 0 -0.4441 0 .3 3 4 6 9 4 No D ifference > 0 -0.4441 0 .6 6 5 3 0 6 No Difference: (R e a c h = R 06 , D a te = N o v -0 4 )-(R e a c h = R 0 6 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 6 7 6 6 2 0 .1 0 7 6 4 5 0 .0 2 0 0 9 7 Power (Alpha=.010) 0 .0 1 4 9 7 5 0 .0 2 5 3 6 7 0 .0 0 3 4 4 8 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 06 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 06 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 06 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 06 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1 .6 3 2 6 M odified-Levene E qual-V ariance Test 0 .3 8 1 8 Probability 0.0000 Decision(.050) 1.000000 C annot reject norm ality 0.0000 1.000000 C annot reject norm ality 0 .6 4 6 5 0 6 0 .5 5 3 8 2 3 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R06, Date=Nov-04 Reach=R06, Date=Nov-12 Count 5 5 Median 3.16 4.15 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:18:38 AM 1433 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 06, D ate=N ov-04 R each=R 06, D ate=N ov-12 N u m b e r S ets o f T ie s = 1, Mann W Whitney U Sum Ranks 10 25 15 30 Multiplicity Factor = 6 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 7 2 6 0 7 4.77 2 6 0 7 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0 .5 2 3 8 0.600402 No -0 .5 2 3 8 0.300201 No -0 .5 2 3 8 0.699799 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.4191 0.675174 No -0.4191 0.337587 No -0 .6 2 8 6 0.735190 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .4 0 0 0 0 0 0 .2 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .8 7 3 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:38 AM Normal Probability Plot of Channei_Catfish_PFOS_ppb when Reach=R0B, Date=No 1434 1 5.0 5 10 25 50 75 Percent of Values 90 95 99 Channel_Catfish_PFOS_ppb vs Groups 2.0 Reach=R06, Date=Nov-04 Reach=R06, Date=Nov-12 Groups 4/10/2013 11:18:48 AM 1486 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 06, D ate=D ec-06 5 6 .0 2 5 .4 6 9 3 2 8 2.44 5 9 5 8 -0 .7 7 1 0 6 7 8 1 2 .8 1 1 0 7 R each=R 06, D ate=N ov-12 5 3 .7 0 4 0 .9 1 8 5 2 0 6 0 .4 1 0 7 7 4 9 2.56 3 5 0 6 4 .8 4 4 4 9 4 Note: T-alp h a (R each = R 06, D ate= D ec-06) = 2 .7 7 6 4 , T-alpha (R each = R 06, D ate=N o v-12) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 2.316 3 .9 2 1 5 5 8 Unequal 4.23 2.316 5 .5 4 5 9 2 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .7 1 9 0 Standard Error 2 .4 8 0 2 1 1 2 .4 8 0 2 1 1 95.0% LCL Difference -3 .4 0 3 3 7 7 -4 .4 2 7 6 6 95.0% UCL Difference 8 .0 3 5 3 7 7 9 .0 5 9 6 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .9 3 3 8 0 .3 7 7 7 3 5 No D ifference < 0 0 .9 3 3 8 0 .8 1 1 1 3 3 No D ifference > 0 0 .9 3 3 8 0 .1 8 8 8 6 7 No Difference: (R e a c h = R 0 6 , D ate= D e c -0 6 )-(R e a c h =R 06, Date=:N ov-12) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 3 1 0 6 0 0 .0 0 6 1 1 2 0 .2 1 5 1 0 5 Power (Alpha=.010) 0 .0 3 5 1 0 3 0 .0 0 0 8 8 8 0 .0 6 1 9 6 0 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .9 3 3 8 0 .4 0 0 6 3 6 No D ifference < 0 0 .9 3 3 8 0 .7 9 9 6 8 2 No D ifference > 0 0 .9 3 3 8 0 .2 0 0 3 1 8 No Difference: (R e a c h = R 06 , D a te = D e c -0 6 )-(R e a c h = R 0 6 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 1 4 6 1 0 0 .0 0 7 1 2 8 0 .1 9 6 4 2 3 Power (Alpha=.010) 0 .0 2 7 0 7 8 0 .0 0 1 1 8 7 0 .0 4 9 9 6 6 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 06 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 06 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 06 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 06 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 3 5 .4 5 6 0 M odified-Levene E qual-V ariance Test 2 .6 9 0 8 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 4 4 3 2 0 .1 3 9 5 5 8 R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R06, Date=Dec-06 Reach=R06, Date=Nov-12 Count 5 5 Median 4.29 4.15 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:18:48 AM 1487 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 06, D ate=D ec-06 R each=R 06, D ate=N ov-12 N u m b e r S ets o f T ie s = 1, Mann W Whitney U Sum Ranks 15 30 10 25 Multiplicity F acto r = 6 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 7 2 6 0 7 4.77 2 6 0 7 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0 .5 2 3 8 0.600402 No 0 .5 2 3 8 0.699799 No 0 .5 2 3 8 0.300201 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0 .4 1 9 1 0.675174 No 0 .6 2 8 6 0.735190 No 0 .4 1 9 1 0.337587 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .2 0 0 0 0 0 0 .4 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .8 7 3 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:49 AM Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R0B, Date=D 1488 Channel_Catflsh_PFOS_ppb vs Groups 16 i 4/10/2013 11:19:29 AM 1684 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 09, Date=Jun-02 6 1 1 3 .4 4 8 .0 8 1 0 5 1 9 .6 2 9 0 1 6 2 .9 4 2 0 2 R each=R 09, D ate=N ov-04 5 1 6 .3 0 4 1 0 .7 8 6 3 1 4.82 3 7 8 4 2.91 1 0 2 7 2 9 .6 9 6 9 7 Note: T-alp h a (R each = R 09, D ate=Ju n -02) = 2 .5 7 0 6 , T -alpha (R each = R 09, D ate=N o v-04) = 2 .7 7 6 4 1 6 3 .8 5 8 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 97.096 3 6 .5 5 1 8 2 Unequal 5.60 97.096 4 9 .2 7 6 0 8 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .4 9 0 3 Standard Error 22.1 3 3 2 2 20.2 1 3 0 4 95.0% LCL Difference 47.0 2 7 1 7 4 6 .7 5 9 4 1 95.0% UCL Difference 1 4 7 .1 6 4 8 1 4 7 .4 3 2 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 4 .3 8 6 9 0 .0 0 1 7 5 3 Yes D ifference < 0 4 .3 8 6 9 0 .9 9 9 1 2 3 No D ifference > 0 4 .3 8 6 9 0 .0 0 0 8 7 7 Yes Difference: (R e a c h = R 09 , D ate=Ju n -02)-(R each =:R 09, Date= N ov-04) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 7 2 8 4 2 0 .0 0 0 0 0 0 0 .9 9 1 4 3 8 Power (Alpha=.010) 0 .8 3 6 1 3 5 0 .0 0 0 0 0 0 0 .9 1 4 3 1 2 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 4 .8 0 3 6 0 .0 0 3 6 0 8 Yes D ifference < 0 4 .8 0 3 6 0 .9 9 8 1 9 6 No D ifference > 0 4 .8 0 3 6 0 .0 0 1 8 0 4 Yes Difference: (R e a c h = R 09 , D a te = J u n -0 2 )-(R e a c h = R 0 9 , D ate=N o v-04) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 7 3 1 3 5 0 .0 0 0 0 0 0 0 .9 9 3 7 4 8 Power (Alpha=.010) 0 .7 8 1 2 2 9 0 .0 0 0 0 0 0 0 .8 9 3 6 1 7 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 09 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 9 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 09 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 09 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 09 , D ate= N o v-04) Variance-R atio E qual-V ariance Test 1 9 .8 7 0 2 M odified-Levene E qual-V ariance Test 4.0521 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 1 2 6 0 3 0 .0 7 4 9 6 6 R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R09, Date=Jun-02 Reach=R09, Date=Nov-04 Count 6 5 Median 98.9 11.2 95.0% LCL of Median 59.1 95.0% UCL of Median 178 4/10/2013 11:19:29 AM 1685 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 09, Date=Jun-02 R each=R 09, D ate=N ov-04 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 30 51 0 15 Multiplicity F acto r = 0 Mean of W 36 30 Std Dev of W 5 .4 7 7 2 2 6 5 .4 7 7 2 2 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.004329 Yes 0.997835 No 0.002165 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2 .7 3 8 6 0.006170 Yes -2 .7 3 8 6 0.996915 No -2 .7 3 8 6 0.003085 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2 .6 4 7 3 0.008113 Yes -2 .8 2 9 9 0.997672 No -2 .6 4 7 3 0.004057 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 1 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 0 4 3 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:19:29 AM Normal Probability' Plot of Channel_Catfish_PFOS_ppb when Reach=R09, Date=Ju 1686 40J--- 1---------- 1----- 1----- --i---------- 1---------- 1---------1----- 1---------- i1 5 10 25 50 75 90 95 99 Percent of Values Channel_Catfish_PFOS_ppb vs Groups 180 0J---------------------1------------------------------------------1------------- Reach=R09, Date=Jun-02 Reach=R09, Date=Nov-04 Groups 4/10/2013 11:19:29 AM 1687 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 09, Date=Jun-02 6 1 1 3 .4 48.0 8 1 0 5 1 9 .6 2 9 0 1 62.9 4 2 0 2 R each=R 09, D ate=D ec-06 5 3 8 .5 1 9 .9 5 9 8 4 8.92631 1 3 .7 1 6 5 9 6 3 .2 8 3 4 1 Note: T-alp h a (R each = R 09, D ate=Ju n -02) = 2 .5 7 0 6 , T -alpha (R each = R 09, D ate= D ec-06) = 2 .7 7 6 4 1 6 3 .8 5 8 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 74.9 3 8 .2 2 8 1 5 Unequal 6.91 7 4 .9 5 2 .0 5 9 4 2 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .3 7 0 7 Standard Error 23.1 4 8 2 9 21.5 6 3 3 3 95.0% LCL Difference 22.5 3 4 9 3 2 3 .7 7 9 4 95.0% UCL Difference 1 2 7 .2 6 5 1 1 2 6 .0 2 0 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 3 .2 3 5 7 0 .0 1 0 2 2 9 Yes D ifference < 0 3 .2 3 5 7 0 .9 9 4 8 8 5 No D ifference > 0 3 .2 3 5 7 0 .0 0 5 1 1 5 Yes Difference: (R e a c h = R 09 , D ate=Ju n -02)-(R each =:R 09, Date= D ec-06) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .8 2 0 4 8 4 0 .0 0 0 0 0 2 0 .9 0 8 9 9 7 Power (Alpha=.010) 0 .5 2 7 4 2 9 0 .0 0 0 0 0 0 0 .6 6 1 5 4 8 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 3 .4 7 3 5 0 .0 1 0 5 6 6 Yes D ifference < 0 3 .4 7 3 5 0 .9 9 4 7 1 7 No D ifference > 0 3 .4 7 3 5 0 .0 0 5 2 8 3 Yes Difference: (R e a c h = R 09 , D a te = J u n -0 2 )-(R e a c h = R 0 9 , D a te = D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .8 4 2 8 0 4 0 .0 0 0 0 0 1 0 .9 2 8 3 6 2 Power (Alpha=.010) 0 .5 3 0 4 8 3 0 .0 0 0 0 0 0 0 .6 7 6 1 1 1 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 09 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 9 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 09 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 09 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 09 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 5 .8 0 2 8 M odified-Levene E qual-V ariance Test 2 .0 0 2 6 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .1 1 3 2 4 7 0 .1 9 0 6 8 5 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R09, Date=Jun-02 Reach=R09, Date=Dec-06 Count 6 5 Median 98.9 47.6 95.0% LCL of Median 59.1 95.0% UCL of Median 178 4/10/2013 11:19:29 AM 1688 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 09, Date=Jun-02 R each=R 09, D ate=D ec-06 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 30 51 0 15 Multiplicity F acto r = 0 Mean of W 36 30 Std Dev of W 5 .4 7 7 2 2 6 5 .4 7 7 2 2 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.004329 Yes 0.997835 No 0.002165 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2 .7 3 8 6 0.006170 Yes -2 .7 3 8 6 0.996915 No -2 .7 3 8 6 0.003085 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2 .6 4 7 3 0.008113 Yes -2 .8 2 9 9 0.997672 No -2 .6 4 7 3 0.004057 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 1 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 0 4 3 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:19:30 AM Normal Probability' Plot of Channel_Catfish_PFOS_ppb when Reach=R09, Date=Ju 1689 40 J-----1--------------1------- 1------- --i-------------- 1--------------1------------ 1------- 1--------------i- 1 5 10 25 50 75 90 95 99 Percent of Values Channel_Catfish_PFOS_ppb vs Groups 180 0J---------------------1------------------------------------------1------------- Reach=R09, Date=Juri-02 Reach=R09, Date=Dec-06 Groups 4/10/2013 11:19:30 AM 1690 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 09, Date=Jun-02 6 1 1 3 .4 4 8 .0 8 1 0 5 1 9 .6 2 9 0 1 6 2 .9 4 2 0 2 R each=R 09, D ate=N ov-12 5 2 2 .4 8 1 3 .4 1 6 1 1 5 .9 9 9 8 6 6 5 .8 2 1 7 Note: T-alp h a (R each = R 09, D ate=Ju n -02) = 2 .5 7 0 6 , T -alpha (R each = R 09, D ate=N o v-12) = 2 .7 7 6 4 1 6 3 .8 5 8 3 9 .1 3 8 3 Confidence-Limits of Difference Section Variance Assumption Mean Standard DF Difference Deviation Equal 9 90.92 3 6 .9 3 6 7 4 Unequal 5.91 9 0 .9 2 4 9 .9 1 7 7 3 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .4 5 5 6 Standard Error 2 2 .3 6 6 3 1 2 0 .5 2 5 5 1 95.0% LCL Difference 40.3 2 3 8 9 4 0 .5 1 7 1 1 95.0% UCL Difference 1 4 1 .5 1 6 1 1 4 1 .3 2 2 9 Equal-Variance T-Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 4 .0 6 5 0 0 .0 0 2 8 2 1 Yes D ifference < 0 4 .0 6 5 0 0 .9 9 8 5 9 0 No D ifference > 0 4 .0 6 5 0 0 .0 0 1 4 1 0 Yes D iffe re n c e : (R e a c h = R 0 9 , D a te = J u n -0 2 )-(R e a c h = :R 0 9 , D a te = :N o v -1 2 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 4 9 8 6 9 0 .0 0 0 0 0 0 0 .9 8 1 7 4 5 Power (Alpha=.010) 0 .7 6 6 0 2 3 0 .0 0 0 0 0 0 0 .8 6 5 0 1 1 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 4 .4 2 9 6 0 .0 0 4 5 8 2 Yes D ifference < 0 4 .4 2 9 6 0 .9 9 7 7 0 9 No D ifference > 0 4 .4 2 9 6 0 .0 0 2 2 9 1 Yes Difference: (R e a c h = R 09 , D a te = J u n -0 2 )-(R e a c h = R 0 9 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 5 3 8 5 6 0 .0 0 0 0 0 0 0 .9 8 6 7 0 5 Power (Alpha=.010) 0 .7 2 5 2 3 9 0 .0 0 0 0 0 0 0 .8 5 1 1 4 5 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 09 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 9 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 09 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 09 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 09 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1 2 .8 4 3 8 M odified-Levene E qual-V ariance Test 3 .7 7 6 6 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 2 8 3 3 8 0 .0 8 3 8 5 8 R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R09, Date=Jun-02 Reach=R09, Date=Nov-12 Count 6 5 Median 98.9 18.6 95.0% LCL of Median 59.1 95.0% UCL of Median 178 4/10/2013 11:19:30 AM 1691 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 09, Date=Jun-02 R each=R 09, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 30 51 0 15 Multiplicity F acto r = 0 Mean of W 36 30 Std Dev of W 5 .4 7 7 2 2 6 5 .4 7 7 2 2 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.004329 Yes 0.997835 No 0.002165 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2 .7 3 8 6 0.006170 Yes -2 .7 3 8 6 0.996915 No -2 .7 3 8 6 0.003085 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2 .6 4 7 3 0.008113 Yes -2 .8 2 9 9 0.997672 No -2 .6 4 7 3 0.004057 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 1 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 0 4 3 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:19:30 AM 1692 Normal Probability' Plot of Channel_Catfish_PFOS_ppb when Reach=R09, Date=Ju Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R09, Date=N 180-i Channel_Catfish_PFOS_ppb vs Groups 0 J---------------------1------------------------------------------1------------- Reach=R09,Date=Jun-02 Reach=R09,Date=Nov-12 Groups 4/10/2013 11:19:37 AM 1726 D a ta s e t V ariable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 09, D ate=N ov-04 5 1 6 .3 0 4 1 0 .7 8 6 3 1 4 .823784 2.91 1 0 2 7 2 9 .6 9 6 9 7 R each=R 09, D ate=D ec-06 5 3 8 .5 1 9 .9 5 9 8 4 8 .9 2 6 3 1 1 3 .7 1 6 5 9 6 3 .2 8 3 4 1 Note: T-alp h a (R each = R 09, D ate=N o v-04) = 2 .7 7 6 4 , T-alpha (R each = R 09, D a te= D ec-06) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -22.196 1 6 .0 4 2 7 5 Unequal 6.15 -22.1 9 6 2 2 .6 8 7 8 7 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .4 3 2 3 Standard Error 1 0 .1 4 6 3 2 1 0 .1 4 6 3 2 95.0% LCL Difference -4 5 .5 9 3 4 6 -4 6 .8 7 4 5 8 95.0% UCL Difference 1 .2 0 1 4 6 6 2.48 2 5 8 4 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .1 8 7 6 0 .0 6 0 1 4 7 No D ifference < 0 -2 .1 8 7 6 0 .0 3 0 0 7 3 Yes D ifference > 0 -2 .1 8 7 6 0 .9 6 9 9 2 7 No D iffe re n c e : (R e a c h = R 0 9 , D a te = N o v -0 4 )-(R e a c h = R 0 9 , D a te := D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 8 5 6 7 2 0 .6 3 7 9 8 7 0 .0 0 0 1 2 2 Power (Alpha=.010) 0 .2 0 6 8 6 2 0 .3 0 9 1 3 2 0 .0 0 0 0 1 2 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .1 8 7 6 0 .0 7 0 1 9 6 No D ifference < 0 -2 .1 8 7 6 0 .0 3 5 0 9 8 Yes D ifference > 0 -2 .1 8 7 6 0 .9 6 4 9 0 2 No D iffe re n c e : (R e a c h = R 0 9 , D a te = N o v -0 4 )-(R e a c h = R 0 9 , D a te := D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 5 4 8 9 3 0 .6 1 6 1 3 3 0 .0 0 0 1 4 3 Power (Alpha=.010) 0 .1 7 5 8 3 1 0 .2 7 4 8 4 2 0 .0 0 0 0 1 7 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 09 , D ate= N ov-04) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 09 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 09 , D ate= D ec-06) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 09 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 3 .4 2 4 3 M odified-Levene E qual-V ariance Test 0.9701 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .2 6 0 3 3 8 0 .3 5 3 5 1 0 C annot reject equal variances C annot reject equal variances Median Statistics Variable R each=R 09, D ate=N ov-04 R each=R 09, D ate=D ec-06 Count 5 5 Median 11.2 4 7 .6 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:19:37 AM 1727 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 09, D ate=N ov-04 R each=R 09, D ate=D ec-06 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 3 18 22 37 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.027778 Yes 0.972222 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1 .9 8 4 5 0.047202 Yes -1 .9 8 4 5 0.023601 Y es -1 .9 8 4 5 0.976399 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1 .8 8 0 0 0.060103 No -1 .8 8 0 0 0.030051 Yes -2 .0 8 8 9 0.981643 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 0 0 0 0 0 0 .6 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:19:37 AM Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R09, Date=N 1728 4/10/2013 11:19:38 AM 1729 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 09, D ate=N ov-04 5 1 6 .3 0 4 1 0 .7 8 6 3 1 4.82 3 7 8 4 2.91 1 0 2 7 2 9 .6 9 6 9 7 R each=R 09, D ate=N ov-12 5 2 2 .4 8 1 3 .4 1 6 1 1 5 .9 9 9 8 6 6 5 .8 2 1 7 Note: T-alp h a (R each = R 09, D ate=N o v-04) = 2 .7 7 6 4 , T-alpha (R each = R 09, D ate=N o v-12) = 2 .7 7 6 4 3 9 .1 3 8 3 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.176 1 2 .1 7 2 4 4 Unequal 7.65 -6.176 1 7 .2 1 4 4 3 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .3 2 4 7 Standard Error 7 .6 9 8 5 2 5 7 .6 9 8 5 2 5 95.0% LCL Difference -2 3 .9 2 8 8 3 -2 4 .0 7 2 4 95.0% UCL Difference 1 1 .5 7 6 8 3 1 1 .7 2 0 4 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .8 0 2 2 0 .4 4 5 5 9 4 No D ifference < 0 -0 .8 0 2 2 0 .2 2 2 7 9 7 No D ifference > 0 -0 .8 0 2 2 0 .7 7 7 2 0 3 No Difference: (R e a c h = R 0 9 , D ate= N o v-0 4 )-(R e a c h =R 09, Date=:N ov-12) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 0 9 4 3 6 0 .1 8 1 6 3 6 0 .0 0 8 5 6 1 Power (Alpha=.010) 0 .0 2 7 8 9 8 0 .0 4 9 5 9 0 0 .0 0 1 2 9 7 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 -0 .8 0 2 2 0 .4 4 6 6 3 0 No D ifference < 0 -0 .8 0 2 2 0 .2 2 3 3 1 5 No D ifference > 0 -0 .8 0 2 2 0 .7 7 6 6 8 5 No Difference: (R e a c h = R 09 , D a te = N o v -0 4 )-(R e a c h = R 0 9 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 0 8 7 4 7 0 .1 8 0 8 5 0 0 .0 0 8 6 2 4 Power (Alpha=.010) 0 .0 2 7 5 3 8 0 .0 4 9 0 6 2 0 .0 0 1 3 1 6 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 09 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 09 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 09 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 09 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1.5471 M odified-Levene E qual-V ariance Test 0 .0 0 8 9 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .6 8 2 7 8 2 0 .9 2 7 0 7 6 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R09, Date=Nov-04 Reach=R09, Date=Nov-12 Count 5 5 Median 11.2 18.6 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:19:38 AM 1730 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 09, D ate=N ov-04 R each=R 09, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 7 22 18 33 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.154762 No 0.845238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1 .1 4 8 9 0.250592 No -1 .1 4 8 9 0.125296 No -1 .1 4 8 9 0.874704 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1 .0 4 4 5 0.296270 No -1 .0 4 4 5 0.148135 No -1 .2 5 3 4 0.894962 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 0 0 0 0 0 0 .6 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Histogram 60 n 60 - , ------------------------------------- Histogram so- 50- 40 - 40 - 20- 10- o -------------,-------------------------- 1-------------------------- ,------------- 5 10 15 20 25 30 35 Channel_Catfish_PFOS_ppbwhenF;each=R09,Date=Nov-04 20 - 10- 0 -L|------------------- ------------------------------------------------------------ 10 20 30 40 SO Channel_Catfish_PFOS_ppbwhenReach=R09,Date=Nov-12 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:19:38 AM Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R09, Date=N 1731 50 n Channel_Catflsh_PFOS_ppb vs Groups 5 J---------------------------1-------------------------------------------------------1---------------- Reach=R09, Date=Nov-04 Reach=R09, Date=Nov-12 Groups 4/10/2013 11:19:45 AM 1765 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 09, D ate=D ec-06 5 3 8 .5 1 9 .9 5 9 8 4 8.92631 1 3 .7 1 6 5 9 6 3 .2 8 3 4 1 R each=R 09, D ate=N ov-12 5 2 2 .4 8 1 3 .4 1 6 1 1 5 .9 9 9 8 6 6 5 .8 2 1 7 Note: T-alpha (R each =R 09, D ate=D ec-06) = 2 .7764, T-alpha (R each =R 09, D ate=N o v-12) = 2 .7764 3 9 .1 3 8 3 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 16.02 1 7 .0 0 5 6 9 Unequal 7 .0 0 1 6 .0 2 2 4 .0 4 9 6 8 Note: T-alpha (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .3 6 4 5 Standard Error 1 0 .7 5 5 3 4 1 0 .7 5 5 3 4 95.0% LCL Difference -8 .7 8 1 8 6 5 -9 .4 1 1 1 2 6 95.0% UCL Difference 40.8 2 1 8 7 4 1 .4 5 1 1 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .4 8 9 5 0 .1 7 4 6 8 6 No D ifference < 0 1 .4 8 9 5 0 .9 1 2 6 5 7 No D ifference > 0 1 .4 8 9 5 0 .0 8 7 3 4 3 No D ifferen ce: (R e a c h = R 0 9 , D a te = D e c -0 6 )-(R e a c h =R 09, D ate=:N o v -1 2 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 5 9 7 7 2 0 .0 0 1 2 6 4 0 .3 8 9 0 9 8 Power (Alpha=.010) 0 .0 8 5 3 7 5 0 .0 0 0 1 5 5 0 .1 4 1 1 7 9 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 1 .4 8 9 5 0 .1 7 9 9 6 0 No D ifference < 0 1 .4 8 9 5 0 .9 1 0 0 2 0 No D ifference > 0 1 .4 8 9 5 0 .0 8 9 9 8 0 No Difference: (R e a c h = R 09 , D a te = D e c -0 6 )-(R e a c h = R 0 9 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 5 2 1 0 5 0 .0 0 1 3 2 5 0 .3 8 2 2 6 8 Power (Alpha=.010) 0 .0 7 9 9 6 4 0 .0 0 0 1 7 0 0 .1 3 4 3 4 4 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 09 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 09 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 09 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 09 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 2 .2 1 3 4 M odified-Levene E qual-V ariance Test 0 .7 2 9 4 Probability 0.0000 Decision(.050) 1.000000 C annot reject norm ality 0.0000 1.000000 C annot reject norm ality 0 .4 6 0 5 1 0 0 .4 1 7 9 0 5 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R09, Date=Dec-06 Reach=R09, Date=Nov-12 Count 5 5 Median 47.6 18.6 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:19:45 AM 1766 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 09, D ate=D ec-06 R each=R 09, D ate=N ov-12 N um ber S ets of Ties = 1, Mann W Whitney U Sum Ranks 19.5 3 4 .5 5.5 20.5 Multiplicity F acto r = 6 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 7 2 6 0 7 4 .7 7 2 6 0 7 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1 .4 6 6 7 0.142457 No 1 .4 6 6 7 0.928772 No 1 .4 6 6 7 0.071228 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1 .3 6 1 9 0.173217 No 1 .5 7 1 5 0.941963 No 1 .3 6 1 9 0.086609 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 0 0 0 0 0 0 .0 0 0 0 0 0 0 .6 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:19:45 AM 1767 4/10/2013 11:20:06 AM 1864 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each= R 11, D ate=N ov-04 5 2 4 .2 8 1 9 .6 6 7 9 2 8 .7 9 5 7 6 3 -0 .1 4 0 9 5 2 1 4 8 .7 0 0 9 5 R each= R 11, D ate=D ec-06 5 1 4 .8 2 3 .2 3 9 9 0 8 1 .4 4 8 9 3 1 1 0 .7 9 7 1 2 1 8 .8 4 2 8 8 Note: T -a lp h a (R e a c h = R 1 1 , D a te = N o v -0 4 ) = 2 .7 7 6 4 , T -alp h a (R e a c h = R 1 1, D a te = D e c -0 6 ) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 9.46 1 4 .0 9 4 7 5 Unequal 4.22 9.46 1 9 .9 3 2 9 9 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .7 2 1 0 Standard Error 8 .9 1 4 3 0 6 8 .9 1 4 3 0 6 95.0% LCL Difference -1 1 .0 9 6 4 3 -1 4 .7 9 5 9 5 95.0% UCL Difference 3 0 .0 1 6 4 2 3 3 .7 1 5 9 5 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .0 6 1 2 0 .3 1 9 5 7 6 No D ifference < 0 1 .0 6 1 2 0 .8 4 0 2 1 2 No D ifference > 0 1 .0 6 1 2 0 .1 5 9 7 8 8 No Difference: (R e a c h = R 1 1 , D ate= N ov-04)-(R each =R11, Date= D ec-06) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 5 5 3 1 3 0 .0 0 4 3 5 2 0 .2 5 0 6 6 8 Power (Alpha=.010) 0 .0 4 3 6 1 1 0 .0 0 0 6 0 8 0 .0 7 6 0 8 6 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 1 .0 6 1 2 0 .3 4 5 5 4 9 No D ifference < 0 1 .0 6 1 2 0 .8 2 7 2 2 6 No D ifference > 0 1 .0 6 1 2 0 .1 7 2 7 7 4 No Difference: (R e a c h = R 1 1 , D a te = N o v -0 4 )-(R e a c h = R 1 1 , D a te = D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 3 3 6 2 4 0 .0 0 5 2 0 9 0 .2 2 7 1 8 7 Power (Alpha=.010) 0 .0 3 2 5 1 4 0 .0 0 0 8 4 9 0 .0 5 9 9 5 5 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 11 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 1 1 , D a te = N o v -0 4 ) O m nibus N orm ality (R e a c h = R 1 1, D a te = N o v -0 4 ) Skew ness Norm ality (R e a c h = R 11 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 1 1 , D a te = D e c -0 6 ) O m nibus N orm ality (R e a c h = R 1 1, D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 3 6 .8 5 1 2 M odified-Levene E qual-V ariance Test 4.3341 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 4 1 1 4 0 .0 7 0 9 1 1 R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R11, Date=Nov-04 Reach=R11, Date=Dec-06 Count 5 5 Median 15.6 13.4 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:20:06 AM 1865 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each= R 11, D ate=N ov-04 R each= R 11, D ate=D ec-06 N um ber S ets of Ties = 1, Mann W Whitney U Sum Ranks 13.5 2 8 .5 11.5 2 6 .5 Multiplicity F acto r = 6 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 7 2 6 0 7 4.77 2 6 0 7 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0 .2 0 9 5 0.834035 No 0 .2 0 9 5 0.582982 No 0 .2 0 9 5 0.417018 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0 .1 0 4 8 0.916563 No 0 .3 1 4 3 0.623351 No 0 .1 0 4 8 0.458281 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .4 0 0 0 0 0 0 .4 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .8 7 3 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal ProbabilityPlot ofChannel_Catfish_PFOS_ppbwhenReach=R11,Date=N 4/10/2013 11:20:06 AM 1866 Groups 4/10/2013 11:20:06 AM 1867 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each= R 11, D ate=N ov-04 5 2 4 .2 8 1 9 .6 6 7 9 2 8 .7 9 5 7 6 3 -0 .1 4 0 9 5 2 1 4 8 .7 0 0 9 5 R each=R 11, D ate=N ov-12 5 1 5 .3 1 8 5 .3 5 5 0 0 9 2.39 4 8 3 3 8 .6 6 8 8 7 9 2 1 .9 6 7 1 2 Note: T -a lp h a (R e a c h = R 1 1 , D a te = N o v -0 4 ) = 2 .7 7 6 4 , T -alp h a (R e a c h = R 1 1, D a te = N o v -1 2) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 8.962 1 4 .4 1 3 5 9 Unequal 4.59 8.962 2 0 .3 8 3 9 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .6 4 1 2 Standard Error 9 .1 1 5 9 5 6 9 .1 1 5 9 5 6 95.0% LCL Difference -1 2 .0 5 9 4 3 -1 5 .1 1 5 4 4 95.0% UCL Difference 29.9 8 3 4 3 3 3 .0 3 9 4 4 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .9 8 3 1 0 .3 5 4 3 4 6 No D ifference < 0 0 .9 8 3 1 0 .8 2 2 8 2 7 No D ifference > 0 0 .9 8 3 1 0 .1 7 7 1 7 3 No Difference: (R e a c h = R 1 1 , D ate= N o v-0 4 )-(R e a c h =R 11, Date=:N ov-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 4 0 0 6 1 0 .0 0 5 3 6 8 0 .2 2 8 5 1 3 Power (Alpha=.010) 0 .0 3 8 2 0 9 0 .0 0 0 7 6 8 0 .0 6 7 1 6 6 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .9 8 3 1 0 .3 7 4 4 9 4 No D ifference < 0 0 .9 8 3 1 0 .8 1 2 7 5 3 No D ifference > 0 0 .9 8 3 1 0 .1 8 7 2 4 7 No Difference: (R e a c h = R 11 , D a te = N o v -0 4 )-(R e a c h = R 1 1 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 2 4 4 8 5 0 .0 0 6 1 6 3 0 .2 1 1 3 2 1 Power (Alpha=.010) 0 .0 3 0 3 0 9 0 .0 0 0 9 9 8 0 .0 5 5 5 9 8 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 11 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 1 1 , D a te = N o v -0 4 ) O m nibus N orm ality (R e a c h = R 1 1, D a te = N o v -0 4 ) Skew ness Norm ality (R e a c h = R 11 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 1 1 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 11 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1 3 .4 8 9 5 M odified-Levene E qual-V ariance Test 3 .1 5 6 8 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 2 7 2 6 4 0 .1 1 3 5 1 4 R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R11, Date=Nov-04 Reach=R11, Date=Nov-12 Count 5 5 Median 15.6 15.7 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:20:07 AM 1868 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each= R 11, D ate=N ov-04 R each=R 11, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 12 27 13 28 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 1.000000 No 0.500000 No 0.500000 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0 .1 0 4 4 0.916815 No -0 .1 0 4 4 0.458407 No -0 .1 0 4 4 0.541593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0 .0 0 0 0 1.000000 No 0 .0 0 0 0 0.500000 No -0 .2 0 8 9 0.582734 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .4 0 0 0 0 0 0 .4 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .8 7 3 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal ProbabilityPlot ofChannel_Catfish_PFOS_ppbwhenReach=R11,Date=N 4/10/2013 11:20:07 AM 1869 Groups 4/10/2013 11:20:11 AM 1891 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 11, D ate=D ec-06 5 1 4 .8 2 3.23 9 9 0 8 1.448931 1 0 .7 9 7 1 2 1 8 .8 4 2 8 8 R each=R 11, D ate=N ov-12 5 1 5 .3 1 8 5.35 5 0 0 9 2 .394833 8 .6 6 8 8 7 9 2 1 .9 6 7 1 2 Note: T -a lp h a (R e a c h = R 1 1 , D a te = D e c -0 6 ) = 2 .7 7 6 4 , T -alp h a (R e a c h = R 1 1, D a te = N o v -1 2) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -0.498 4 .4 2 5 6 7 1 Unequal 6.58 -0.498 6 .2 5 8 8 4 3 Note: T-alpha (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .3 9 5 4 Standard Error 2 .7 9 9 0 4 2 .7 9 9 0 4 95.0% LCL Difference -6 .9 5 2 5 9 8 -7 .2 0 2 7 5 8 95.0% UCL Difference 5 .9 5 6 5 9 7 6 .2 0 6 7 5 8 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .1 7 7 9 0 .8 6 3 2 1 0 No D ifference < 0 -0 .1 7 7 9 0 .4 3 1 6 0 5 No D ifference > 0 -0 .1 7 7 9 0 .5 6 8 3 9 5 No D iffe re n c e : (R e a c h = R 1 1 , D a te = D e c -0 6 )- (R e a c h = R 1 1 , D a te := N o v -1 2 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 5 2 8 5 3 0 .0 6 9 2 5 2 0 .0 3 5 2 6 5 Power (Alpha=.010) 0 .0 1 0 7 9 3 0 .0 1 4 8 0 1 0 .0 0 6 6 1 1 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .1 7 7 9 0 .8 6 4 1 3 8 No D ifference < 0 -0 .1 7 7 9 0 .4 3 2 0 6 9 No D ifference > 0 -0 .1 7 7 9 0 .5 6 7 9 3 1 No D iffe re n c e : (R e a c h = R 1 1 , D a te = D e c -0 6 )- (R e a c h = R 1 1 , D a te := N o v -1 2 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 5 2 7 0 9 0 .0 6 8 8 6 2 0 .0 3 5 4 8 7 Power (Alpha=.010) 0 .0 1 0 7 3 0 0 .0 1 4 6 1 6 0 .0 0 6 7 0 1 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 11 , D ate= D ec-06) Kurtosis N orm ality (R e a c h = R 1 1 , D a te = D e c -0 6 ) O m nibus N orm ality (R e a c h = R 1 1, D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 11 , D ate= N ov-12) Kurtosis N orm ality (R e a c h = R 1 1 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 11 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 2 .7 3 1 8 M odified-Levene E qual-V ariance Test 1 .1 1 1 7 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .3 5 3 8 6 5 0 .3 2 2 5 2 3 C annot reject equal variances C annot reject equal variances Median Statistics Variable R each=R 11, D ate=D ec-06 R each= R 11, D ate=N ov-12 Count 5 5 Median 13.4 15.7 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:20:12 AM 1892 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 11, D ate=D ec-06 R each=R 11, D ate=N ov-12 N um ber S ets of Ties = 1, Mann W Whitney U Sum Ranks 11.5 2 6 .5 13.5 2 8 .5 Multiplicity F acto r = 6 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 7 2 6 0 7 4.77 2 6 0 7 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0 .2 0 9 5 0.834035 No -0 .2 0 9 5 0.417018 No -0 .2 0 9 5 0.582982 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0 .1 0 4 8 0.916563 No -0 .1 0 4 8 0.458281 No -0 .3 1 4 3 0.623351 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .4 0 0 0 0 0 0 .2 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .8 7 3 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal ProbabilityPlot ofChannel_Catfish_PFOS_ppbwhenReach=R11,Date=D 4/10/2013 11:20:12 AM 1893 Channel_Catflsh_PFOS_ppbvsGroups 24n Reach=R11,Date=Dec-06 Reach=R11,Date=Nov-12 Groups 4/10/2013 11:20:24 AM 1954 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 13, D ate=Jul-07 6 8 .2 1 6 6 6 6 4.08 8 7 2 4 1 .6 6 9 2 1 5 3 .9 2 5 8 1 4 1 2 .5 0 7 5 2 R each=R 13, D ate=Jul-08 6 4 .0 6 1 6 6 6 2.81 4 5 9 4 1 .1 4 9 0 5 3 1 .1 0 7 9 3 2 7.015401 Note: T-alp h a (R each = R 13, D ate=Jul-07) = 2 .5 7 0 6 , T-alpha (R each = R 13, D ate=Ju l-08) = 2 .5 7 0 6 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 4.155 3 .5 0 9 9 5 8 Unequal 8.87 4.155 4 .9 6 3 8 3 Note: T-alp h a (Equal) = 2 .2 2 8 1 , T-alpha (U nequal) = 2 .2 6 7 2 Standard Error 2.02 6 4 7 5 2.02 6 4 7 5 95.0% LCL Difference -0 .3 6 0 2 6 7 5 -0 .4 3 9 4 8 9 4 95.0% UCL Difference 8 .6 7 0 2 6 7 8 .7 4 9 4 9 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .0 5 0 4 0 .0 6 7 4 6 8 No D ifference < 0 2 .0 5 0 4 0 .9 6 6 2 6 6 No D ifference > 0 2 .0 5 0 4 0 .0 3 3 7 3 4 Yes Difference: (R e a c h = R 13 , D ate= Ju l-07)-(R each = R 13, D ate=Ju l-08) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 5 7 5 0 7 0 .0 0 0 1 7 9 0 .6 0 4 2 1 0 Power (Alpha=.010) 0 .1 9 7 4 5 4 0 .0 0 0 0 1 7 0 .2 9 2 2 0 7 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .0 5 0 4 0 .0 7 1 0 3 0 No D ifference < 0 2 .0 5 0 4 0 .9 6 4 4 8 5 No D ifference > 0 2 .0 5 0 4 0 .0 3 5 5 1 5 Yes Difference: (R e a c h = R 13 , D ate = J u l-0 7 )-(R e a c h = R 1 3 , D ate=Ju l-08) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 4 7 6 6 1 0 .0 0 0 1 8 9 0 .5 9 7 1 7 5 Power (Alpha=.010) 0 .1 8 6 9 4 3 0 .0 0 0 0 1 9 0 .2 8 0 9 8 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 13 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 13 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 13 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 13 , D ate=Ju l-08) Variance-R atio E qual-V ariance Test 2 .1 1 0 3 M odified-Levene E qual-V ariance Test 0 .9 5 0 4 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .4 3 1 8 4 3 0 .3 5 2 6 1 6 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-07 Reach=R13, Date=Jul-08 Count 6 6 Median 7.85 4.33 95.0% LCL of Median 2 .9 0 .0 9 95.0% UCL of Median 14.4 7 .5 7 4/10/2013 11:20:25 AM 1955 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 13, D ate=Jul-07 R each= R 13, D ate=Jul-08 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 29 50 7 28 Multiplicity F acto r = 0 Mean of W 39 39 Std Dev of W 6 .2 4 4 9 9 8 6.24 4 9 9 8 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.093074 No 0.953463 No 0.046537 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1 .7 6 1 4 0.078169 No 1 .7 6 1 4 0.960915 No 1 .7 6 1 4 0.039085 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1 .6 8 1 3 0.092696 No 1 .8 4 1 5 0.967224 No 1 .6 8 1 3 0.046348 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .5 0 0 0 0 0 0 .0 0 0 0 0 0 0 .5 0 0 0 0 0 Reject H0 if Greater Than 0 .6 9 8 0 0 .6 9 8 0 0 .6 9 8 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .4 7 4 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal ProbabilityPlot of Channel_Catfish_PFOS_ppbwhenReach=R13,Date=J 4/10/2013 11:20:25 AM 1956 Channel_Catflsh_PFOS_ppbvsGroups 16i 4/10/2013 11:20:25 AM 1957 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 13, D ate=Jul-07 6 8 .2 1 6 6 6 6 4.08 8 7 2 4 1 .6 6 9 2 1 5 3 .9 2 5 8 1 4 1 2 .5 0 7 5 2 R each=R 13, D ate=Jul-09 6 1 6 .2 6 3 3 3 6.98 6 3 7 2 2.85 2 1 7 4 8 .9 3 1 5 8 6 2 3 .5 9 5 0 8 Note: T-alp h a (R each = R 13, D ate=Jul-07) = 2 .5 7 0 6 , T-alpha (R each = R 13, D ate=Ju l-09) = 2 .5 7 0 6 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -8.046667 5 .7 2 3 9 4 3 Unequal 8.07 -8 .0 4 6 6 6 7 8 .0 9 4 8 7 8 Note: T-alp h a (Equal) = 2 .2 2 8 1 , T-alpha (U nequal) = 2 .3 0 2 7 Standard Error 3 .3 0 4 7 2 3 .3 0 4 7 2 95.0% LCL Difference -1 5 .4 1 0 0 4 -1 5 .6 5 6 6 1 95.0% UCL Difference -0 .6 8 3 2 9 1 2 -0 .4 3 6 7 2 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .4 3 4 9 0 .0 3 5 1 5 4 Yes D ifference < 0 -2 .4 3 4 9 0 .0 1 7 5 7 7 Yes D ifference > 0 -2 .4 3 4 9 0 .9 8 2 4 2 3 No Difference: (R e a c h = R 13 , D ate= Ju l-07)-(R each = R 13, D ate=Ju l-09) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .5 9 4 0 9 4 0 .7 3 2 5 8 9 0 .0 0 0 0 4 2 Power (Alpha=.010) 0 .2 9 7 2 4 5 0 .4 1 4 0 1 1 0 .0 0 0 0 0 4 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .4 3 4 9 0 .0 4 0 6 5 2 Yes D ifference < 0 -2 .4 3 4 9 0 .0 2 0 3 2 6 Yes D ifference > 0 -2 .4 3 4 9 0 .9 7 9 6 7 4 No Difference: (R e a c h = R 13 , D ate = J u l-0 7 )-(R e a c h = R 1 3 , D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .5 7 1 9 6 1 0 .7 1 8 7 3 5 0 .0 0 0 0 4 8 Power (Alpha=.010) 0 .2 6 7 7 2 8 0 .3 8 5 0 2 3 0 .0 0 0 0 0 5 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 13 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 13 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 13 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 13 , D ate=Ju l-09) Variance-R atio E qual-V ariance Test 2 .9 1 9 6 M odified-Levene E qual-V ariance Test 1 .1 6 7 2 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .2 6 4 5 9 0 0 .3 0 5 3 5 4 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-07 Reach=R13, Date=Jul-09 Count 6 6 Median 7.85 18.36 95.0% LCL of Median 2 .9 6 .0 3 95.0% UCL of Median 14.4 2 3 .0 3 4/10/2013 11:20:25 AM 1958 D a ta s e t V ariable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 13, D ate=Jul-07 R each= R 13, D ate=Jul-09 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 6 27 30 51 Multiplicity F acto r = 0 Mean of W 39 39 Std Dev of W 6 .2 4 4 9 9 8 6.24 4 9 9 8 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.064935 No 0.032468 Yes 0.967532 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1 .9 2 1 5 0.054664 No -1 .9 2 1 5 0.027332 Yes -1 .9 2 1 5 0.972668 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1 .8 4 1 5 0.065552 No -1 .8 4 1 5 0.032776 Yes -2 .0 0 1 6 0.977336 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 6 6 6 6 7 0 .6 6 6 6 6 7 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 9 8 0 0 .6 9 8 0 0 .6 9 8 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .1 4 2 9 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal ProbabilityPlot of Channel_Catfish_PFOS_ppbwhenReach=R13,Date=J 4/10/2013 11:20:25 AM 1959 Channel_Catfish_PFOS_ppbvsGroups 24.0 -, 2.0 -I---------------------1------------------------------------------1------------ Reach=R13, Date=Jul-07 Reach=R13, Date=Jul-09 Groups 4/10/2013 11:20:26 AM 1960 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each= R 13, D ate=Jul-07 6 8 .2 1 6 6 6 6 4.08 8 7 2 4 1 .6 6 9 2 1 5 3 .9 2 5 8 1 4 1 2 .5 0 7 5 2 R each=R 13, D ate=N ov-12 5 9 .7 7 2 1 .6 3 5 6 2 5 0 .7 3 1 4 7 3 9 7 .7 4 1 1 0 3 Note: T-alp h a (R each = R 13, D ate=Jul-07) = 2 .5 7 0 6 , T-alpha (R each = R 13, D ate =N o v-12) = 2 .7 7 6 4 1 1 .8 0 2 9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 -1 .5 5 5 3 3 3 3 .2 3 6 7 5 8 Unequal 6.79 -1 .5 5 5 3 3 3 4 .4 0 3 7 4 1 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .3 7 9 4 Standard Error 1 .9 5 9 9 5 4 1 .8 2 2 4 5 2 95.0% LCL Difference -5 .9 8 9 0 5 8 -5 .8 9 1 6 9 5 95.0% UCL Difference 2 .8 7 8 3 9 1 2.78 1 0 2 8 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0 .7 9 3 6 0 .4 4 7 8 7 0 No D ifference < 0 -0 .7 9 3 6 0 .2 2 3 9 3 5 No D ifference > 0 -0 .7 9 3 6 0 .7 7 6 0 6 5 No Difference: (R e a c h = R 13 , D ate= Ju l-07)-(R each = R 13, D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 0 9 7 8 4 0 .1 8 1 4 2 5 0 .0 0 8 5 9 8 Power (Alpha=.010) 0 .0 2 8 3 5 9 0 .0 5 0 1 3 1 0 .0 0 1 2 8 2 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 -0 .8 5 3 4 0 .4 2 2 4 9 6 No D ifference < 0 -0 .8 5 3 4 0 .2 1 1 2 4 8 No D ifference > 0 -0 .8 5 3 4 0 .7 8 8 7 5 2 No Difference: (R e a c h = R 13 , D ate = J u l-0 7 )-(R e a c h = R 1 3 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 1 4 4 6 4 0 .1 9 0 9 1 4 0 .0 0 7 7 4 8 Power (Alpha=.010) 0 .0 2 8 9 6 5 0 .0 5 1 8 8 1 0 .0 0 1 1 9 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 13 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 13 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 13 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 13 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 6 .2 4 9 0 M odified-Levene E qual-V ariance Test 3.9641 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .1 0 0 1 8 9 0 .0 7 7 6 7 0 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-07 Reach=R13, Date=Nov-12 Count 6 5 Median 7.85 10.3 95.0% LCL of Median 2 .9 95.0% UCL of Median 14.4 4/10/2013 11:20:26 AM 1961 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 13, D ate=Jul-07 R each=R 13, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 11 3 2 19 34 Multiplicity F acto r = 0 Mean of W 36 30 Std Dev of W 5 .4 7 7 2 2 6 5 .4 7 7 2 2 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.536797 No 0.268398 No 0.731602 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0 .7 3 0 3 0.465209 No 0 .7 3 0 3 0.232604 No 0 .7 3 0 3 0.767396 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0 .6 3 9 0 0.522817 No 0 .6 3 9 0 0.261408 No 0 .8 2 1 6 0.794343 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .5 0 0 0 0 0 0 .5 0 0 0 0 0 0 .1 6 6 6 6 7 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal ProbabilityPlot of Channel_Catfish_PFOS_ppbwhenReach=R13,Date=J 4/10/2013 11:20:26 AM 1962 Channel_Catflsh_PFOS_ppbvsGroups 16i 14 - Groups 4/10/2013 11:20:27 AM 1969 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 13, D ate=Jul-08 6 4 .0 6 1 6 6 6 2.81 4 5 9 4 1 .1 4 9 0 5 3 1 .1 0 7 9 3 2 7.015401 R each=R 13, D ate=Jul-09 6 1 6 .2 6 3 3 3 6.98 6 3 7 2 2.85 2 1 7 4 8 .9 3 1 5 8 6 2 3 .5 9 5 0 8 Note: T-alp h a (R each = R 13, D ate=Jul-08) = 2 .5 7 0 6 , T-alpha (R each = R 13, D ate=Ju l-09) = 2 .5 7 0 6 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 10 -1 2 .2 0 1 6 7 5 .3 2 5 9 4 3 Unequal 6.58 -1 2 .2 0 1 6 7 7 .5 3 2 0 2 Note: T-alp h a (Equal) = 2 .2 2 8 1 , T-alpha (U nequal) = 2 .3 9 5 5 Standard Error 3 .0 7 4 9 3 4 3 .0 7 4 9 3 4 95.0% LCL Difference -1 9 .0 5 3 0 5 -1 9 .5 6 7 5 4 95.0% UCL Difference -5 .3 5 0 2 8 6 -4 .8 3 5 7 8 8 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -3.9681 0 .0 0 2 6 5 1 Yes D ifference < 0 -3.9681 0 .0 0 1 3 2 6 Yes D ifference > 0 -3.9681 0 .9 9 8 6 7 4 No Difference: (R e a c h = R 13 , D ate= Ju l-08)-(R each = R 13, D ate=Ju l-09) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 4 5 7 9 2 0 .9 7 9 1 5 5 0.000000 Power (Alpha=.010) 0 .7 6 4 8 9 2 0 .8 6 1 1 0 8 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -3.9681 0 .0 0 6 1 2 3 Yes D ifference < 0 -3.9681 0 .0 0 3 0 6 2 Yes D ifference > 0 -3.9681 0 .9 9 6 9 3 8 No Difference: (R e a c h = R 13 , D ate = J u l-0 8 )-(R e a c h = R 1 3 , D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 1 7 3 8 0 0 .9 6 9 7 3 8 0.000000 Power (Alpha=.010) 0 .6 5 2 2 1 0 0 .7 8 7 8 5 2 0.000000 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 13 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 13 , D ate=Ju l-08) Skew ness Norm ality (R e a c h = R 13 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 13 , D ate=Ju l-09) Variance-R atio E qual-V ariance Test 6 .1 6 1 3 M odified-Levene E qual-V ariance Test 2 .7 5 8 2 Probability 0.0000 Decision(.050) 1.000000 C annot reject norm ality 0.0000 1.000000 C annot reject norm ality 0 .0 6 7 6 5 1 0 .1 2 7 7 4 3 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-08 Reach=R13, Date=Jul-09 Count 6 6 Median 4.33 18.36 95.0% LCL of Median 0 .0 9 6 .0 3 95.0% UCL of Median 7 .5 7 2 3 .0 3 4/10/2013 11:20:28 AM 1970 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 13, D ate=Jul-08 R each= R 13, D ate=Jul-09 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 2 23 34 55 Multiplicity F acto r = 0 Mean of W 39 39 Std Dev of W 6 .2 4 4 9 9 8 6.24 4 9 9 8 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.008658 Yes 0.004329 Yes 0.995671 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.5621 0.010406 Yes -2.5621 0.005203 Yes -2.5621 0.994797 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2 .4 8 2 0 0.013065 Yes -2 .4 8 2 0 0.006533 Yes -2.6421 0.995880 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .8 3 3 3 3 3 0 .8 3 3 3 3 3 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 9 8 0 0 .6 9 8 0 0 .6 9 8 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 2 6 0 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:20:28 AM 1971 Groups 4/10/2013 11:20:28 AM 1972 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each= R 13, D ate=Jul-08 6 4 .0 6 1 6 6 6 2.81 4 5 9 4 1 .1 4 9 0 5 3 1 .1 0 7 9 3 2 7.015401 R each=R 13, D ate=N ov-12 5 9 .7 7 2 1 .6 3 5 6 2 5 0 .7 3 1 4 7 3 9 7 .7 4 1 1 0 3 Note: T-alp h a (R each = R 13, D ate=Jul-08) = 2 .5 7 0 6 , T-alpha (R each = R 13, D ate =N o v-12) = 2 .7 7 6 4 1 1 .8 0 2 9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 -5 .7 1 0 3 3 3 2 .3 6 4 3 3 6 Unequal 8.19 -5 .7 1 0 3 3 3 3 .2 5 5 3 3 5 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .2 9 6 6 Standard Error 1 .4 3 1 6 7 7 1 .3 6 2 1 2 2 95.0% LCL Difference -8.949011 -8 .8 3 8 6 2 9 95.0% UCL Difference -2 .4 7 1 6 5 6 -2 .5 8 2 0 3 8 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -3 .9 8 8 6 0 .0 0 3 1 6 5 Yes D ifference < 0 -3 .9 8 8 6 0 .0 0 1 5 8 2 Yes D ifference > 0 -3 .9 8 8 6 0 .9 9 8 4 1 8 No Difference: (R e a c h = R 13 , D ate= Ju l-08)-(R each = R 13, D ate=N o v-12) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 4 2 5 7 7 0 .9 7 8 3 8 8 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .7 4 7 1 5 2 0 .8 5 0 8 4 8 0 .0 0 0 0 0 0 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 -4 .1 9 2 2 0 .0 0 2 8 7 3 Yes D ifference < 0 -4 .1 9 2 2 0 .0 0 1 4 3 7 Yes D ifference > 0 -4 .1 9 2 2 0 .9 9 8 5 6 3 No Difference: (R e a c h = R 13 , D ate = J u l-0 8 )-(R e a c h = R 1 3 , D ate=N o v-12) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 5 5 9 5 1 0 .9 8 5 0 4 2 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .7 7 3 4 3 7 0 .8 7 3 4 2 7 0 .0 0 0 0 0 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 13 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 13 , D ate=Ju l-08) Skew ness Norm ality (R e a c h = R 13 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 13 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 2 .9 6 1 2 M odified-Levene E qual-V ariance Test 2 .0 2 6 9 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .3 1 5 1 2 1 0 .1 8 8 2 6 2 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-08 Reach=R13, Date=Nov-12 Count 6 5 Median 4.33 10.3 95.0% LCL of Median 0 .0 9 95.0% UCL of Median 7 .5 7 4/10/2013 11:20:28 AM 1973 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 13, D ate=Jul-08 R each=R 13, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 21 30 45 Multiplicity F acto r = 0 Mean of W 36 30 Std Dev of W 5 .4 7 7 2 2 6 5 .4 7 7 2 2 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.004329 Yes 0.002165 Yes 0.997835 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2 .7 3 8 6 0.006170 Yes 2 .7 3 8 6 0.003085 Yes 2 .7 3 8 6 0.996915 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 2 .6 4 7 3 0.008113 Yes 2 .6 4 7 3 0.004057 Yes 2 .8 2 9 9 0.997672 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 0 4 3 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:20:28 AM 1974 4/10/2013 11:20:30 AM 1981 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each= R 13, D ate=Jul-09 6 1 6 .2 6 3 3 3 6.98 6 3 7 2 2.85 2 1 7 4 8 .9 3 1 5 8 6 2 3 .5 9 5 0 8 R each=R 13, D ate=N ov-12 5 9 .7 7 2 1 .6 3 5 6 2 5 0 .7 3 1 4 7 3 9 7 .7 4 1 1 0 3 Note: T-alp h a (R each = R 13, D ate=Jul-09) = 2 .5 7 0 6 , T-alpha (R each = R 13, D ate =N o v-12) = 2 .7 7 6 4 1 1 .8 0 2 9 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 9 6.491333 5 .3 2 0 2 7 6 Unequal 5.65 6.4 9 1 3 3 3 7 .1 7 5 2 8 1 Note: T-alp h a (Equal) = 2 .2 6 2 2 , T-alpha (U nequal) = 2 .4 8 4 3 Standard Error 3 .2 2 1 5 8 7 2.94 4 4 7 8 95.0% LCL Difference -0 .7 9 6 4 0 2 8 -0 .8 2 3 5 3 0 6 95.0% UCL Difference 1 3 .7 7 9 0 7 1 3 .8 0 6 2 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .0 1 4 9 0 .0 7 4 7 2 9 No D ifference < 0 2 .0 1 4 9 0 .9 6 2 6 3 6 No D ifference > 0 2 .0 1 4 9 0 .0 3 7 3 6 4 Yes Difference: (R e a c h = R 13 , D ate= Ju l-09)-(R each = R 13, D ate=N o v-12) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 3 6 5 3 6 0 .0 0 0 2 1 3 0 .5 8 5 4 4 2 Power (Alpha=.010) 0 .1 8 0 6 0 1 0 .0 0 0 0 2 2 0 .2 7 2 3 9 1 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 2 .2 0 4 6 0 .0 7 2 4 4 5 No D ifference < 0 2 .2 0 4 6 0 .9 6 3 7 7 8 No D ifference > 0 2 .2 0 4 6 0 .0 3 6 2 2 2 Yes Difference: (R e a c h = R 13 , D ate = J u l-0 9 )-(R e a c h = R 1 3 , D ate=N o v-12) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 4 8 7 1 5 0 .0 0 0 1 4 3 0 .6 1 3 2 4 1 Power (Alpha=.010) 0 .1 6 8 2 3 2 0 .0 0 0 0 1 7 0 .2 6 6 7 3 8 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 13 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 13 , D ate=Ju l-09) Skew ness Norm ality (R e a c h = R 13 , D ate= N o v-12) Kurtosis N orm ality (R e a c h = R 1 3 , D a te = N o v -1 2 ) O m nibus Norm ality (R e a c h = R 13 , D ate= N o v-12) Variance-R atio E qual-V ariance Test 1 8 .2 4 4 7 M odified-Levene E qual-V ariance Test 4 .2 3 8 3 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 1 4 7 9 6 0 .0 6 9 6 2 6 R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R13, Date=Jul-09 Reach=R13, Date=Nov-12 Count 6 5 Median 18.36 10.3 95.0% LCL of Median 6 .0 3 95.0% UCL of Median 2 3 .0 3 4/10/2013 11:20:30 AM 1982 D a ta s e t V ariable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 13, D ate=Jul-09 R each=R 13, D ate=N ov-12 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 22 43 8 23 Multiplicity F acto r = 0 Mean of W 36 30 Std Dev of W 5 .4 7 7 2 2 6 5 .4 7 7 2 2 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.246753 No 0.876623 No 0.123377 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1 .2 7 8 0 0.201243 No -1 .2 7 8 0 0.899379 No -1 .2 7 8 0 0.100621 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1 .1 8 6 7 0.235333 No -1 .3 6 9 3 0.914548 No -1 .1 8 6 7 0.117667 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 6 6 6 6 7 0 .1 6 6 6 6 7 0 .6 6 6 6 6 7 Reject H0 if Greater Than 0 .7 2 4 6 0 .7 2 4 6 0 .7 2 4 6 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .1 0 8 2 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:20:30 AM 1983 Groups APPENDIX F-4 CHANNEL CATFISH STATISTICS SPATIAL EVALUATION 4/10/2013 11:15:50 AM 448 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, Date=Jun-02 6 1 5 .2 3 3 3 3 1 3 .9 8 0 0 8 5 .7 0 7 3 4 4 0 .5 6 2 1 3 8 2 9 .9 0 4 5 3 R each=R 06, Date=Jun-02 7 2.5 0 0 2.5 2.5 Note: T-alpha (R each =R 02, D ate=Jun-02) = 2 .5706, T-alpha (R each =R 06, D ate=Jun-02) = 0.0000 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 11 1 2 .7 3 3 3 3 9 .4 2 5 3 6 8 Unequal 5 .0 0 1 2 .7 3 3 3 3 1 3 .9 8 0 0 8 Note: T-alpha (Equal) = 2 .2 0 1 0 , T-alpha (U nequal) = 2 .5 7 0 6 Standard Error 5.243791 5 .7 0 7 3 4 4 95.0% LCL Difference 1 .1 9 1 8 2 7 -1 .9 3 7 8 6 2 95.0% UCL Difference 2 4 .2 7 4 8 4 2 7 .4 0 4 5 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .4 2 8 3 0 .0 3 3 5 1 2 Yes D ifference < 0 2 .4 2 8 3 0 .9 8 3 2 4 4 No D ifference > 0 2 .4 2 8 3 0 .0 1 6 7 5 6 Yes Difference: (R e a c h = R 02 , D ate=Ju n -02)-(R each = R 06, D ate=Ju n -02) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .6 0 0 1 2 1 0.000041 0 .7 3 5 7 0 8 Power (Alpha=.010) 0 .3 0 7 1 3 9 0 .0 0 0 0 0 3 0 .4 2 3 0 4 8 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 2 .2 3 1 0 0 .0 7 6 0 6 5 No D ifference < 0 2 .2 3 1 0 0 .9 6 1 9 6 7 No D ifference > 0 2 .2 3 1 0 0 .0 3 8 0 3 3 Yes Difference: (R e a c h = R 02 , D a te = J u n -0 2 )-(R e a c h = R 0 6 , D ate=Ju n -02) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 3 8 6 3 8 0 .0 0 0 1 4 2 0 .6 0 8 2 2 3 Power (Alpha=.010) 0 .1 5 6 8 4 0 0 .0 0 0 0 1 8 0 .2 5 4 1 5 8 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 2 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 02 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 06 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 6 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 06 , D ate=Ju n -02) Variance-R atio E qual-V ariance Test 9999800001 M odified-Levene E qual-V ariance Test 1 3 .5 1 3 0 Probability 0 .0 0 0 0 0 .0 0 0 0 0000 0.003651 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 0 0 R eject equal variances R eject equal variances Median Statistics Variable Reach=R02, Date=Jun-02 Reach=R06, Date=Jun-02 Count 6 7 Median 14.35 2.5 95.0% LCL of Median 1 2 .5 95.0% UCL of Median 3 7 .4 2 .5 4/10/2013 11:15:51 AM 449 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, Date=Jun-02 R each=R 06, Date=Jun-02 N um ber Sets of Ties = 2, Mann W Whitney U Sum Ranks 28 49 14 42 Multiplicity F acto r = 3 4 2 Mean of W 42 49 Std Dev of W 6 .4 2 8 6 0 2 6 .4 2 8 6 0 2 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1 .0 8 8 9 0.276205 No 1 .0 8 8 9 0.861897 No 1 .0 8 8 9 0.138103 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.0111 0.311966 No 1 .1 6 6 7 0.878326 No 1.0111 0.155983 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .6 6 6 6 6 7 0 .3 3 3 3 3 3 0 .6 6 6 6 6 7 Reject H0 if Greater Than 0 .6 7 7 5 0 .6 7 7 5 0 .6 7 7 5 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .0 9 0 9 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R02, Date=J 4/10/2013 11:15:51 AM 450 Channel_Catflsh_PFOS_ppb vs Groups 40 n 4/10/2013 11:18:29 AM 1384 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 06, Date=Jun-02 7 2.5 0 0 2.5 2.5 R each=R 09, Date=Jun-02 6 1 1 3 .4 4 8 .0 8 1 0 5 1 9 .6 2 9 0 1 6 2 .9 4 2 0 2 Note: T-alpha (R each =R 06, D ate=Jun-02) = 0.0000, T-alpha (R each =R 09, D ate=Jun-02) = 2 .5706 1 6 3 .8 5 8 Confidence-Limits of Difference Section Variance Assumption Mean Standard DF Difference Deviation Equal 11 -1 1 0 .9 3 2 .4 1 6 2 4 Unequal 5.00 -110.9 4 8 .0 8 1 0 5 Note: T-alp h a (Equal) = 2 .2 0 1 0 , T-alpha (U nequal) = 2 .5 7 0 6 Standard Error 1 8 .0 3 4 7 3 1 9 .6 2 9 0 1 95.0% LCL Difference -1 5 0 .5 9 4 2 -1 6 1 .3 5 8 95.0% UCL Difference -7 1 .2 0 5 8 3 -6 0 .4 4 2 0 2 Equal-Variance T-Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 -6 .1 4 9 2 0 .0 0 0 0 7 2 Yes D ifference < 0 -6 .1 4 9 2 0 .0 0 0 0 3 6 Yes D ifference > 0 -6 .1 4 9 2 0 .9 9 9 9 6 4 No Difference: (R e a c h = R 06 , D ate=Ju n -02)-(R each = R 09, D ate=Ju n -02) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 9 9 8 3 7 0 .9 9 9 9 7 8 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .9 9 4 9 3 2 0 .9 9 8 6 2 6 0 .0 0 0 0 0 0 Aspin-Welch Unequal-Variance Test Section Alternative Hypothesis T-Value Prob Level Reject H0 at .050 D ifference <> 0 -5 .6 4 9 8 0 .0 0 2 4 1 2 Yes D ifference < 0 -5 .6 4 9 8 0 .0 0 1 2 0 6 Yes D ifference > 0 -5 .6 4 9 8 0 .9 9 8 7 9 4 No Difference: (R e a c h = R 06 , D a te = J u n -0 2 )-(R e a c h = R 0 9 , D ate=Ju n -02) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 9 2 7 5 9 0 .9 9 9 0 3 4 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .8 7 0 5 0 4 0 .9 5 1 7 4 8 0 .0 0 0 0 0 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 06 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 6 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 06 , D ate=Ju n -02) Skew ness Norm ality (R e a c h = R 09 , D ate=Ju n -02) Kurtosis N orm ality (R e a c h = R 0 9 , D ate = J u n -0 2 ) O m nibus Norm ality (R e a c h = R 09 , D ate=Ju n -02) Variance-R atio E qual-V ariance Test 0 .0 0 0 0 M odified-Levene E qual-V ariance Test 9 .9 5 3 0 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 0 0 0 .0 0 9 1 6 3 R eject equal variances R eject equal variances Median Statistics Variable Reach=R06, Date=Jun-02 Reach=R09, Date=Jun-02 Count 7 6 Median 2.5 98.9 95.0% LCL of Median 2 .5 59.1 95.0% UCL of Median 2 .5 178 4/10/2013 11:18:29 AM 1385 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 06, Date=Jun-02 R each=R 09, Date=Jun-02 N um ber S ets of Ties = 1, Mann W Whitney U Sum Ranks 0 28 42 63 Multiplicity F acto r = 3 3 6 Mean of W 49 42 Std Dev of W 6 .4 3 9 0 6 4 6 .4 3 9 0 6 4 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 3 .2 6 1 3 0.001109 Yes 3 .2 6 1 3 0.000554 Yes 3 .2 6 1 3 0.999446 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 3 .1 8 3 7 0.001454 Yes 3 .1 8 3 7 0.000727 Yes 3 .3 3 9 0 0.999580 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 7 7 5 0 .6 7 7 5 0 .6 7 7 5 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 0 1 2 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:29 AM 1386 4/10/2013 11:16:02 AM 523 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, D ate=N ov-04 5 3 .8 1 5 2 2 .1 3 8 9 5 9 0 .9 5 6 5 7 1 5 1 .1 5 9 3 3 2 6 .4 7 1 0 6 8 R each=R 05, D ate=N ov-04 10 410.1 1 8 8 .3 1 5 5 9 .5 5 0 4 4 2 7 5 .3 8 7 5 5 4 4 .8 1 2 4 Note: T-alpha (R each =R 02, D ate=N o v-04) = 2 .7764, T-alpha (R each =R 05, D ate=N o v-04) = 2 .2622 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 -406.2848 1 5 6 .6 9 2 1 Unequal 9.00 -4 0 6 .2 8 4 8 1 8 8 .3 2 7 2 Note: T-alp h a (Equal) = 2 .1 6 0 4 , T-alpha (U nequal) = 2 .2 6 2 0 Standard Error 8 5 .8 2 3 7 8 5 9 .5 5 8 1 2 95.0% LCL Difference -5 9 1 .6 9 5 8 -5 4 1 .0 0 4 95.0% UCL Difference -2 2 0 .8 7 3 8 -2 7 1 .5 6 5 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -4 .7 3 3 9 0.000391 Yes D ifference < 0 -4 .7 3 3 9 0 .0 0 0 1 9 5 Yes D ifference > 0 -4 .7 3 3 9 0 .9 9 9 8 0 5 No D ifference: (R each = R 0 2 , D a te = N o v -0 4 )-(R e a c h =R 05, Date= N o v-04) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 9 1 8 4 2 0.997641 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .9 3 6 9 5 5 0 .9 7 0 5 6 5 0 .0 0 0 0 0 0 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -6 .8 2 1 7 0 .0 0 0 0 7 7 Yes D ifference < 0 -6 .8 2 1 7 0 .0 0 0 0 3 8 Yes D ifference > 0 -6 .8 2 1 7 0 .9 9 9 9 6 2 No Difference: (R e a c h = R 02 , D a te = N o v -0 4 )-(R e a c h = R 0 5 , D ate=N o v-04) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 9 9 9 7 2 0 .9 9 9 9 9 8 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .9 9 7 8 7 6 0 .9 9 9 5 8 6 0 .0 0 0 0 0 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 02 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-04) Variance-R atio E qual-V ariance Test 7 7 5 1 .1 2 9 8 M odified-Levene E qual-V ariance Test 1 0 .8 3 9 2 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality -0 .5 9 0 5 -0 .8 4 9 7 1 .0 7 0 7 0 .0 0 0 0 0 0 0 .0 0 5 8 3 3 0 .5 5 4 8 6 5 C annot reject norm ality 0 .3 9 5 4 7 6 C annot reject norm ality 0 .5 8 5 4 6 1 C annot reject norm ality R eject equal variances R eject equal variances Median Statistics Variable Reach=R02, Date=Nov-04 Reach=R05, Date=Nov-04 Count 5 10 Median 4.36 404 95.0% LCL 95.0% UCL of Median of Median 147 608 4/10/2013 11:16:02 AM 524 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, D ate=N ov-04 R each=R 05, D ate=N ov-04 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 50 105 Multiplicity F acto r = 0 Mean of W 40 80 Std Dev of W 8 .1 6 4 9 6 6 8 .1 6 4 9 6 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.000666 Yes 0.000333 Yes 0.999667 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3 .0 6 1 9 0.002200 Yes -3 .0 6 1 9 0.001100 Yes -3 .0 6 1 9 0.998900 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3 .0 0 0 6 0.00 2 6 9 4 Yes -3 .0 0 0 6 0.00 1 3 4 7 Yes -3.1231 0.99 9 1 0 5 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 6 9 8 0 .6 6 9 8 0 .6 6 9 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 0 0 7 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:16:02 AM 525 4/10/2013 11:16:05 AM 544 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, D ate=N ov-04 5 3 .8 1 5 2 2 .1 3 8 9 5 9 0 .9 5 6 5 7 1 5 1 .1 5 9 3 3 2 6 .4 7 1 0 6 8 R each=R 06, D ate=N ov-04 5 3 .4 0 8 1 .1 7 3 6 1 4 0 .5 2 4 8 5 6 2 1 .9 5 0 7 6 6 4 .8 6 5 2 3 4 Note: T-alp h a (R each = R 02, D ate=N o v-04) = 2 .7 7 6 4 , T-alpha (R each = R 06, D ate=N o v-04) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 0.4072 1 .7 2 5 1 8 3 Unequal 6.21 0 .4 0 7 2 2 .4 3 9 7 7 8 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2.4271 Standard Error 1 .0 9 1 1 0 2 1 .0 9 1 1 0 2 95.0% LCL Difference -2 .1 0 8 8 8 5 -2 .2 4 1 0 6 6 95.0% UCL Difference 2.92 3 2 8 5 3 .0 5 5 4 6 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .3 7 3 2 0 .7 1 8 6 9 4 No D ifference < 0 0 .3 7 3 2 0 .6 4 0 6 5 3 No D ifference > 0 0 .3 7 3 2 0 .3 5 9 3 4 7 No O o Z Difference: (R e a c h = R 02 , D ate= N ov-04)-(R each =R06, Date= T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 6 2 6 1 7 0 .0 2 3 3 8 9 0 .0 9 6 4 3 0 Power (Alpha=.010) 0 .0 1 3 5 5 8 0 .0 0 4 0 9 2 0 .0 2 2 2 0 9 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 0 .3 7 3 2 0 .7 2 1 4 1 1 No D ifference < 0 0 .3 7 3 2 0 .6 3 9 2 9 4 No D ifference > 0 0 .3 7 3 2 0 .3 6 0 7 0 6 No Difference: (R e a c h = R 02 , D a te = N o v -0 4 )-(R e a c h = R 0 6 , D ate=N o v-04) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .0 6 1 7 6 2 0 .0 2 3 8 2 7 0 .0 9 5 0 1 5 Power (Alpha=.010) 0 .0 1 3 1 7 7 0 .0 0 4 2 5 3 0 .0 2 1 4 7 4 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 02 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 06 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 06 , D ate= N o v-04) Variance-R atio E qual-V ariance Test 3 .3 2 1 7 M odified-Levene E qual-V ariance Test 0 .5 1 7 5 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .2 7 1 6 9 9 0 .4 9 2 3 7 9 C annot reject equal variances C annot reject equal variances Median Statistics Variable Reach=R02, Date=Nov-04 Reach=R06, Date=Nov-04 Count 5 5 Median 4.36 3.16 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:16:05 AM 545 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, D ate=N ov-04 R each=R 06, D ate=N ov-04 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 17 32 8 23 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.789683 No 0.210318 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0 .9 4 0 0 0.347208 No 0 .9 4 0 0 0.826396 No 0 .9 4 0 0 0.173604 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0 .8 3 5 6 0.403395 No 1 .0 4 4 5 0.851865 No 0 .8 3 5 6 0.201698 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0.200000 0 .4 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .8 7 3 0 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:16:05 AM 546 4/10/2013 11:17:14 AM 964 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=N ov-04 10 410.1 1 8 8 .3 1 5 5 9 .5 5 0 4 4 2 7 5 .3 8 7 5 5 4 4 .8 1 2 4 R each=R 06, D ate=N ov-04 5 3 .4 0 8 1 .1 7 3 6 1 4 0 .5 2 4 8 5 6 2 1 .9 5 0 7 6 6 4 .8 6 5 2 3 4 Note: T-alpha (R each =R 05, D ate=N o v-04) = 2 .2622, T-alpha (R each =R 06, D ate=N o v-04) = 2 .7764 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 406.692 1 5 6 .6 8 8 9 Unequal 9.00 406 .6 9 2 1 8 8 .3 1 8 7 Note: T-alpha (Equal) = 2 .1604, T-alpha (U nequal) = 2.2621 Standard Error 8 5 .8 2 2 0 5 5 9 .5 5 2 7 5 95.0% LCL Difference 2 2 1 .2 8 4 7 2 7 1 .9 7 7 5 95.0% UCL Difference 5 9 2 .0 9 9 3 5 4 1 .4 0 6 5 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 4 .7 3 8 8 0 .0 0 0 3 8 7 Yes D ifference < 0 4 .7 3 8 8 0 .9 9 9 8 0 6 No D ifference > 0 4 .7 3 8 8 0 .0 0 0 1 9 4 Yes O o Z Difference: (R e a c h = R 05 , D ate= N ov-04)-(R each =R06, Date= T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 9 1 9 4 0 0 .0 0 0 0 0 0 0 .9 9 7 6 7 4 Power (Alpha=.010) 0 .9 3 7 4 6 4 0 .0 0 0 0 0 0 0 .9 7 0 8 4 9 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 6.8291 0 .0 0 0 0 7 6 Yes D ifference < 0 6.8291 0 .9 9 9 9 6 2 No D ifference > 0 6.8291 0 .0 0 0 0 3 8 Yes Difference: (R e a c h = R 05 , D a te = N o v -0 4 )-(R e a c h = R 0 6 , D ate=N o v-04) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 9 9 9 7 3 0 .0 0 0 0 0 0 0 .9 9 9 9 9 8 Power (Alpha=.010) 0 .9 9 7 9 1 0 0 .0 0 0 0 0 0 0 .9 9 9 5 9 4 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 05 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 06 , D ate= N o v-04) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 06 , D ate= N o v-04) Variance-R atio E qual-V ariance Test 2 5 7 4 6 .5 6 3 7 M odified-Levene E qual-V ariance Test 1 0 .9 1 7 9 Probability -0 .5 9 0 5 -0 .8 4 9 7 1 .0 7 0 7 0 .0 0 0 0 Decision(.050) 0 .5 5 4 8 6 5 C annot reject norm ality 0 .3 9 5 4 7 6 C annot reject norm ality 0 .5 8 5 4 6 1 C annot reject norm ality 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 0 0 0.005701 R eject equal variances R eject equal variances Median Statistics Variable Reach=R05, Date=Nov-04 Reach=R06, Date=Nov-04 Count 10 5 Median 404 3.16 95.0% LCL of Median 147 95.0% UCL of Median 608 4/10/2013 11:17:14 AM 965 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=N ov-04 R each=R 06, D ate=N ov-04 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 50 105 0 15 Multiplicity Factor = 0 Mean of W 80 40 Std Dev of W 8 .1 6 4 9 6 6 8 .1 6 4 9 6 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.000666 Y es 0.999667 No 0.000333 Y es Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3 .0 6 1 9 0.002200 Y es -3 .0 6 1 9 0.998900 No -3 .0 6 1 9 0.001100 Y es Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3 .0 0 0 6 0.002694 Yes -3.1231 0.999105 No -3 .0 0 0 6 0.001347 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 1 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 6 9 8 0 .6 6 9 8 0 .6 6 9 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 0 0 7 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:14 AM 966 4/10/2013 11:18:40 AM 1444 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 06, D ate=N ov-04 5 3 .4 0 8 1 .1 7 3 6 1 4 0.52 4 8 5 6 2 1 .9 5 0 7 6 6 4 .8 6 5 2 3 4 R each=R 09, D ate=N ov-04 5 1 6 .3 0 4 1 0 .7 8 6 3 1 4 .823784 2.91 1 0 2 7 2 9 .6 9 6 9 7 Note: T-alp h a (R each = R 06, D ate=N o v-04) = 2 .7 7 6 4 , T-alpha (R each = R 09, D ate=N o v-04) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -12.896 7 .6 7 2 0 8 7 Unequal 4.09 -12.8 9 6 1 0 .8 4 9 9 7 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .7 5 1 3 Standard Error 4.85 2 2 5 4 4.85 2 2 5 4 95.0% LCL Difference -2 4 .0 8 5 3 2 -2 6 .2 4 6 0 3 95.0% UCL Difference -1 .7 0 6 6 8 2 0 .4 5 4 0 2 8 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .6 5 7 7 0 .0 2 8 9 0 7 Yes D ifference < 0 -2 .6 5 7 7 0 .0 1 4 4 5 3 Yes D ifference > 0 -2 .6 5 7 7 0 .9 8 5 5 4 7 No D iffe re n c e : (R e a c h = R 0 6 , D a te = N o v -0 4 )-(R e a c h = R 0 9 , D a te := N o v -0 4 ) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .6 4 5 1 3 3 0 .7 8 2 0 3 9 0 .0 0 0 0 2 0 Power (Alpha=.010) 0 .3 2 6 6 2 1 0 .4 5 4 8 9 6 0 .0 0 0 0 0 2 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .6 5 7 7 0 .0 5 5 1 6 9 No D ifference < 0 -2 .6 5 7 7 0 .0 2 7 5 8 5 Yes D ifference > 0 -2 .6 5 7 7 0 .9 7 2 4 1 5 No D iffe re n c e : (R e a c h = R 0 6 , D a te = N o v -0 4 )-(R e a c h = R 0 9 , D a te := N o v -0 4 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .5 2 8 3 9 6 0 .7 0 8 7 2 1 0 .0 0 0 0 3 6 Power (Alpha=.010) 0 .1 9 2 6 8 2 0 .3 1 2 2 3 1 0 .0 0 0 0 0 5 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 06 , D ate= N ov-04) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 06 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 09 , D ate= N ov-04) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 09 , D ate= N o v-04) Variance-R atio E qual-V ariance Test 8 4 .4 6 8 6 M odified-Levene E qual-V ariance Test 2 .9 3 1 6 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 8 1 5 0 .1 2 5 2 1 8 R eject equal variances C annot reject equal variances Median Statistics Variable R each=R 06, D ate=N ov-04 R each=R 09, D ate=N ov-04 Count 5 5 Median 3 .1 6 11.2 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:18:40 AM 1445 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 06, D ate=N ov-04 R each=R 09, D ate=N ov-04 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2 .6 1 1 2 0.009023 Yes -2 .6 1 1 2 0.004512 Yes -2 .6 1 1 2 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2 .5 0 6 7 0.012186 Yes -2 .5 0 6 7 0.006093 Yes -2 .7 1 5 6 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 0 7 9 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:40 AM Normal Probability Plot of Channei_Catfish_PFOS_ppb when Reach=R0B, Date=No 1446 Channel_Catfish_PFOS_ppb vs Groups 35 n 30 25 - 20- 15 - 10- 0J----------------- 1----------------------------------- 1---------- Reach=R0B, Date=Nov-04 Reach=R09, Date=Nov-04 Groups 4/10/2013 11:19:39 AM 1735 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 09, D ate=N ov-04 5 1 6 .3 0 4 1 0 .7 8 6 3 1 4 .823784 2.91 1 0 2 7 2 9 .6 9 6 9 7 R each= R 11, D ate=N ov-04 5 2 4 .2 8 1 9 .6 6 7 9 2 8 .7 9 5 7 6 3 -0 .1 4 0 9 5 2 1 4 8 .7 0 0 9 5 Note: T -a lp h a (R e a c h = R 0 9 , D a te = N o v -0 4 ) = 2 .7 7 6 4 , T -alp h a (R e a c h = R 1 1, D a te = N o v -0 4 ) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -7.976 1 5 .8 6 1 4 6 Unequal 6.21 -7 .9 7 6 2 2 .4 3 1 4 9 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .4 2 7 3 Standard Error 1 0 .0 3 1 6 7 1 0 .0 3 1 6 7 95.0% LCL Difference -3 1 .1 0 9 0 6 -3 2 .3 2 5 9 6 95.0% UCL Difference 1 5 .1 5 7 0 6 1 6 .3 7 3 9 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0.7951 0 .4 4 9 5 0 9 No D ifference < 0 -0.7951 0 .2 2 4 7 5 4 No D ifference > 0 -0.7951 0 .7 7 5 2 4 6 No D iffe re n c e : (R e a c h = R 0 9 , D a te = N o v -0 4 )-(R e a c h = R 1 1 , D a te := N o v -0 4 ) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 0 8 3 6 0 0 .1 7 9 9 1 5 0 .0 0 8 7 1 6 Power (Alpha=.010) 0 .0 2 7 5 4 9 0 .0 4 8 9 7 8 0 .0 0 1 3 2 3 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -0.7951 0 .4 5 5 8 8 7 No D ifference < 0 -0.7951 0 .2 2 7 9 4 4 No D ifference > 0 -0.7951 0 .7 7 2 0 5 6 No D iffe re n c e : (R e a c h = R 0 9 , D a te = N o v -0 4 )-(R e a c h = R 1 1 , D a te := N o v -0 4 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .1 0 4 2 6 0 0 .1 7 5 1 4 7 0 .0 0 9 1 0 7 Power (Alpha=.010) 0 .0 2 5 4 8 3 0 .0 4 5 8 9 4 0 .0 0 1 4 4 5 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 09 , D ate= N ov-04) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = N o v -0 4 ) O m nibus Norm ality (R e a c h = R 09 , D ate= N o v-04) Skew ness Norm ality (R e a c h = R 11 , D ate= N ov-04) Kurtosis N orm ality (R e a c h = R 1 1 , D a te = N o v -0 4 ) O m nibus N orm ality (R e a c h = R 1 1, D a te = N o v -0 4 ) Variance-R atio E qual-V ariance Test 3 .3 2 4 8 M odified-Levene E qual-V ariance Test 1.0881 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .2 7 1 3 3 4 0 .3 2 7 3 7 9 C annot reject equal variances C annot reject equal variances Median Statistics Variable R each=R 09, D ate=N ov-04 R each= R 11, D ate=N ov-04 Count 5 5 Median 11.2 15.6 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:19:39 AM 1736 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 09, D ate=N ov-04 R each= R 11, D ate=N ov-04 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 11 2 6 14 29 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.841270 No 0.420635 No 0.579365 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0 .3 1 3 3 0.75 4 0 2 3 No -0 .3 1 3 3 0.377011 No -0 .3 1 3 3 0.62 2 9 8 9 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0 .2 0 8 9 0.834532 No -0 .2 0 8 9 0.417266 No -0 .4 1 7 8 0.661948 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .4 0 0 0 0 0 0 .4 0 0 0 0 0 0 .2 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) No No No Prob Level 0 .8 7 3 0 Plots Section Histogram 60 n 60 - , -------------------------------------------------- Histogram so- 50 - 40 - 40 - 20- 10- o --------------,---------------------------- 1---------------------------- ,-------------- 5 10 15 20 25 30 35 Channel_Catfish_PFOS_ppb w hen F;each=R09, Date=Nov-04 20- 10- 0 -L5|-----------1,5-----------25-----------35,-----------45-----------551-----------65 Channel_Catfish_PFOS_ppb w he n Reach=R11, Date=Nov-04 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:19:39 AM Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R09, Date=N 1737 Channel_Catflsh_PFOS_ppb vs Groups 50 n 5 J--------------------- 1-------------------------------------------1------------- Reach=R09, Date=Nov-04 Reach=R11, Date=Nov-04 Groups 4/10/2013 11:16:17 AM 616 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 02, D ate=D ec-06 5 6 .0 9 4 2 .9 8 9 7 9 6 1 .3 3 7 0 7 7 2 .3 8 1 6 7 8 9 .8 0 6 3 2 2 R each=R 05, D ate=D ec-06 10 6 9 6 .8 3 8 2 .4 6 8 1 2 0 .9 4 7 4 2 3 .1 9 8 9 9 7 0 .4 0 1 1 Note: T-alpha (R each =R 02, D ate=D ec-06) = 2 .7764, T-alpha (R each =R 05, D ate=D ec-06) = 2 .2622 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 -690.706 3 1 8 .2 3 6 9 Unequal 9.00 -6 9 0 .7 0 6 3 8 2 .4 7 9 6 Note: T-alp h a (Equal) = 2 .1 6 0 4 , T-alpha (U nequal) = 2.2621 Standard Error 1 7 4 .3 0 5 5 1 2 0 .9 5 4 4 95.0% LCL Difference -1 0 6 7 .2 7 -9 6 4 .3 1 3 6 95.0% UCL Difference -3 1 4 .1 4 1 8 -4 1 7 .0 9 8 4 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -3 .9 6 2 6 0 .0 0 1 6 2 2 Yes D ifference < 0 -3 .9 6 2 6 0.000811 Yes D ifference > 0 -3 .9 6 2 6 0 .9 9 9 1 8 9 No II ZeoJn DCD ilCD Difference: (R e a c h = R 02 , D ate= D e c -06)-(R each D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 5 5 0 7 2 0 .9 8 2 1 5 5 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .8 0 8 0 8 2 0 .8 8 6 8 9 5 0 .0 0 0 0 0 0 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -5 .7 1 0 5 0 .0 0 0 2 9 0 Yes D ifference < 0 -5 .7 1 0 5 0 .0 0 0 1 4 5 Yes D ifference > 0 -5 .7 1 0 5 0 .9 9 9 8 5 5 No Difference: (R e a c h = R 02 , D a te = D e c -0 6 )-(R e a c h = R 0 5 , D a te = D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 9 8 9 3 9 0.999831 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .9 7 7 6 6 2 0 .9 9 2 8 0 4 0 .0 0 0 0 0 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 02 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 2 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 02 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 1 6 3 6 4 .6 6 0 2 M odified-Levene E qual-V ariance Test 2 4 .3 1 5 0 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality -0 .0 8 0 4 -1 .8 9 5 7 3.6001 0 .0 0 0 0 0 0 0 .0 0 0 2 7 5 0 .9 3 5 8 8 0 C annot reject norm ality 0 .0 5 8 0 0 1 C annot reject norm ality 0 .1 6 5 2 9 0 C annot reject norm ality R eject equal variances R eject equal variances Median Statistics Variable R each=R 02, D ate=D ec-06 R each=R 05, D ate=D ec-06 Count 5 10 Median 4 .8 8 747 95.0% LCL 95.0% UCL of Median of Median 257 1070 4/10/2013 11:16:17 AM 617 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 02, D ate=D ec-06 R each=R 05, D ate=D ec-06 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 50 105 Multiplicity Factor = 0 Mean of W 40 80 Std Dev of W 8 .1 6 4 9 6 6 8 .1 6 4 9 6 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.000666 Yes 0.000333 Yes 0.999667 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3 .0 6 1 9 0.002200 Yes -3 .0 6 1 9 0.001100 Yes -3 .0 6 1 9 0.998900 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3 .0 0 0 6 0.00 2 6 9 4 Yes -3 .0 0 0 6 0.00 1 3 4 7 Yes -3.1231 0.99 9 1 0 5 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 6 9 8 0 .6 6 9 8 0 .6 6 9 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 0 0 7 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:16:17 AM 618 4/10/2013 11:17:27 AM 1042 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 05, D ate=D ec-06 10 6 9 6 .8 3 8 2 .4 6 8 1 2 0 .9 4 7 4 2 3 .1 9 8 9 9 7 0 .4 0 1 1 R each=R 06, D ate=D ec-06 5 6 .0 2 5 .4 6 9 3 2 8 2 .4 4 5 9 5 8 -0 .7 7 1 0 6 7 8 1 2 .8 1 1 0 7 N o te: T -a lp h a (R e a c h := R 0 5 , D a te = D e c -0 6 ) = 2 .2 6 2 2 , T -a lp h a (R e a c h = R 0 6 , D a te = D e c -0 6 ) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 13 690.78 3 1 8 .2 4 7 Unequal 9.01 6 9 0 .7 8 3 8 2 .5 0 7 Note: T-alpha (Equal) = 2 .1604, T-alpha (U nequal) = 2 .2619 Standard Error 1 7 4 .3 1 1 1 1 2 0 .9 7 1 7 95.0% LCL Difference 3 1 4 .2 0 3 8 4 1 7 .1 5 7 95.0% UCL Difference 1 0 6 7 .3 5 6 9 6 4 .4 0 3 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 3 .9 6 2 9 0 .0 0 1 6 2 2 Yes D ifference < 0 3 .9 6 2 9 0 .9 9 9 1 8 9 No D ifference > 0 3 .9 6 2 9 0.000811 Yes Difference: (R e a c h = R 05 , D ate= D ec-0 6 )-(R e a c h =R06, Date= D ec-06) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 5 5 0 9 8 0 .0 0 0 0 0 0 0 .9 8 2 1 6 8 Power (Alpha=.010) 0 .8 0 8 1 5 2 0 .0 0 0 0 0 0 0 .8 8 6 9 4 6 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 5 .7 1 0 3 0 .0 0 0 2 9 0 Yes D ifference < 0 5 .7 1 0 3 0 .9 9 9 8 5 5 No D ifference > 0 5 .7 1 0 3 0 .0 0 0 1 4 5 Yes Difference: (R e a c h = R 05 , D a te = D e c -0 6 )-(R e a c h = R 0 6 , D a te = D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 9 8 9 4 0 0 .0 0 0 0 0 0 0 .9 9 9 8 3 1 Power (Alpha=.010) 0 .9 7 7 6 8 8 0 .0 0 0 0 0 0 0 .9 9 2 8 1 2 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 05 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 06 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 06 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 4 8 9 0 .1 4 9 6 M odified-Levene E qual-V ariance Test 2 4 .1 0 2 0 Probability -0 .0 8 0 4 -1 .8 9 5 7 3.6001 0 .0 0 0 0 Decision(.050) 0 .9 3 5 8 8 0 C annot reject norm ality 0 .0 5 8 0 0 1 C annot reject norm ality 0 .1 6 5 2 9 0 C annot reject norm ality 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 0 0 0 .0 0 0 2 8 5 R eject equal variances R eject equal variances Median Statistics Variable R each=R 05, D ate=D ec-06 R each=R 06, D ate=D ec-06 Count 10 5 Median 747 4 .2 9 95.0% LCL of Median 257 95.0% UCL of Median 1070 4/10/2013 11:17:27 AM 1043 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 05, D ate=D ec-06 R each=R 06, D ate=D ec-06 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 50 105 0 15 Multiplicity Factor = 0 Mean of W 80 40 Std Dev of W 8 .1 6 4 9 6 6 8 .1 6 4 9 6 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.000666 Yes 0.999667 No 0.000333 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3 .0 6 1 9 0.002200 Yes -3 .0 6 1 9 0.998900 No -3 .0 6 1 9 0.001100 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3 .0 0 0 6 0.00 2 6 9 4 Yes -3.1231 0.99 9 1 0 5 No -3 .0 0 0 6 0.00 1 3 4 7 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 1 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 6 9 8 0 .6 6 9 8 0 .6 6 9 8 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 0 0 7 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:28 AM 1044 Normal Probability Plot of Channel_Catfish_PFOS_ppb when Reach=R05, Date=Dec 1400 n 1200- Channel_Catfish_PFOS_ppb vs Groups 200- 0J------------------- 1--------------------------- I Reach=R05, Date=Dec-06 Reach=R06, Date=Dec-06 Groups 4/10/2013 11:18:51 AM 1501 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 06, D ate=D ec-06 5 6 .0 2 5 .4 6 9 3 2 8 2.44 5 9 5 8 -0 .7 7 1 0 6 7 8 1 2 .8 1 1 0 7 R each=R 09, D ate=D ec-06 5 3 8 .5 1 9 .9 5 9 8 4 8 .9 2 6 3 1 1 3 .7 1 6 5 9 6 3 .2 8 3 4 1 Note: T-alp h a (R each = R 06, D ate= D ec-06) = 2 .7 7 6 4 , T-alpha (R each = R 09, D a te= D ec-06) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -32.48 1 4 .6 3 4 0 1 Unequal 4.60 -32.48 2 0 .6 9 5 6 2 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .6 3 9 8 Standard Error 9 .2 5 5 3 6 2 9 .2 5 5 3 6 2 95.0% LCL Difference -5 3 .8 2 2 9 -5 6 .9 1 2 3 5 95.0% UCL Difference -1 1 .1 3 7 1 -8 .0 4 7 6 5 6 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -3 .5 0 9 3 0 .0 0 7 9 7 0 Yes D ifference < 0 -3 .5 0 9 3 0 .0 0 3 9 8 5 Yes D ifference > 0 -3 .5 0 9 3 0 .9 9 6 0 1 5 No D iffe re n c e : (R e a c h = R 0 6 , D a te = D e c -0 6 )- (R e a c h = R 0 9 , D a te := D e c -0 6 ) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .8 6 5 8 7 5 0 .9 3 9 1 6 5 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .5 8 2 8 8 4 0 .7 1 8 1 6 9 0 .0 0 0 0 0 0 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -3 .5 0 9 3 0 .0 1 9 6 5 0 Yes D ifference < 0 -3 .5 0 9 3 0 .0 0 9 8 2 5 Yes D ifference > 0 -3 .5 0 9 3 0 .9 9 0 1 7 5 No D iffe re n c e : (R e a c h = R 0 6 , D a te = D e c -0 6 )- (R e a c h = R 0 9 , D a te := D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .7 8 1 3 2 5 0 .9 0 2 9 5 2 0 .0 0 0 0 0 1 Power (Alpha=.010) 0 .3 9 7 6 9 5 0 .5 6 3 3 8 7 0 .0 0 0 0 0 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 06 , D ate= D ec-06) Kurtosis N orm ality (R e a c h = R 0 6 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 06 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 09 , D ate= D ec-06) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 09 , D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 1 3 .3 1 8 2 M odified-Levene E qual-V ariance Test 2 .8 2 9 8 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 2 7 9 0 4 0 .1 3 1 0 2 9 R eject equal variances C annot reject equal variances Median Statistics Variable R each=R 06, D ate=D ec-06 R each=R 09, D ate=D ec-06 Count 5 5 Median 4 .2 9 4 7 .6 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:18:52 AM 1502 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 06, D ate=D ec-06 R each=R 09, D ate=D ec-06 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 1 16 24 39 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4.78 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.007937 Yes 0.992063 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2 .4 0 2 3 0.016294 Yes -2 .4 0 2 3 0.008147 Yes -2 .4 0 2 3 0.991853 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2 .2 9 7 8 0.021572 Yes -2 .2 9 7 8 0.010786 Yes -2 .5 0 6 7 0.993907 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .8 0 0 0 0 0 0 .8 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 7 9 4 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:52 AM 1503 4/10/2013 11:19:47 AM 1774 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 09, D ate=D ec-06 5 3 8 .5 1 9 .9 5 9 8 4 8.92631 1 3 .7 1 6 5 9 6 3 .2 8 3 4 1 R each= R 11, D ate=D ec-06 5 1 4 .8 2 3 .2 3 9 9 0 8 1 .4 4 8 9 3 1 1 0 .7 9 7 1 2 1 8 .8 4 2 8 8 Note: T -a lp h a (R e a c h = R 0 9 , D a te = D e c -0 6 ) = 2 .7 7 6 4 , T -alp h a (R e a c h = R 1 1, D a te = D e c -0 6 ) = 2 .7 7 6 4 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 23.68 1 4 .2 9 8 4 6 Unequal 4.21 2 3 .6 8 2 0 .2 2 1 0 8 Note: T-alp h a (Equal) = 2 .3 0 6 0 , T-alpha (U nequal) = 2 .7 2 2 5 Standard Error 9 .0 4 3 1 4 1 9 .0 4 3 1 4 1 95.0% LCL Difference 2.82 6 4 7 9 -0 .9 4 0 1 0 4 7 95.0% UCL Difference 44.5 3 3 5 2 4 8 .3 0 0 1 1 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .6 1 8 6 0 .0 3 0 7 1 9 Yes D ifference < 0 2 .6 1 8 6 0 .9 8 4 6 4 0 No D ifference > 0 2 .6 1 8 6 0 .0 1 5 3 6 0 Yes Difference: (R e a c h = R 09 , D ate= D e c -06)-(R each =R11, Date= D ec-06) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .6 3 2 4 2 0 0 .0 0 0 0 2 3 0 .7 7 1 4 5 4 Power (Alpha=.010) 0 .3 1 5 6 8 1 0 .0 0 0 0 0 2 0 .4 4 2 2 4 5 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 2 .6 1 8 6 0 .0 5 5 9 2 0 No D ifference < 0 2 .6 1 8 6 0 .9 7 2 0 4 0 No D ifference > 0 2 .6 1 8 6 0 .0 2 7 9 6 0 Yes Difference: (R e a c h = R 09 , D a te = D e c -0 6 )-(R e a c h = R 1 1 , D a te = D e c -0 6 ) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .5 2 3 3 5 9 0 .0 0 0 0 4 1 0 .7 0 2 2 3 7 Power (Alpha=.010) 0 .1 9 1 9 1 3 0 .0 0 0 0 0 5 0 .3 0 9 9 9 2 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 09 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 0 9 , D a te = D e c -0 6 ) O m nibus Norm ality (R e a c h = R 09 , D a te = D e c -0 6 ) Skew ness Norm ality (R e a c h = R 11 , D a te = D e c -0 6 ) Kurtosis N orm ality (R e a c h = R 1 1 , D a te = D e c -0 6 ) O m nibus N orm ality (R e a c h = R 1 1, D a te = D e c -0 6 ) Variance-R atio E qual-V ariance Test 3 7 .9 5 3 2 M odified-Levene E qual-V ariance Test 3 .7 9 4 8 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 0 0 1 .0 0 0 0 0 0 C annot reject norm ality 0 .0 0 3 8 8 7 0 .0 8 7 2 6 0 R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R09, Date=Dec-06 Reach=R11, Date=Dec-06 Count 5 5 Median 47.6 13.4 95.0% LCL 95.0% UCL of Median of Median 4/10/2013 11:19:47 AM 1775 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 09, D ate=D ec-06 R each= R 11, D ate=D ec-06 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 21 36 4 19 Multiplicity F acto r = 0 Mean of W 2 7 .5 2 7 .5 Std Dev of W 4 .7 8 7 1 3 6 4.78 7 1 3 6 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.952381 No 0.047619 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1 .7 7 5 6 0.075800 No 1 .7 7 5 6 0.962100 No 1 .7 7 5 6 0.037900 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.6711 0.094693 No 1 .8 8 0 0 0.969949 No 1.6711 0.047346 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .8 0 0 0 0 0 0 .0 0 0 0 0 0 0 .8 0 0 0 0 0 Reject H0 if Greater Than 0 .7 4 9 0 0 .7 4 9 0 0 .7 4 9 0 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes No Yes Prob Level 0 .0 7 9 4 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:19:47 AM 1776 4/10/2013 11:14:50 AM 34 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 01, D ate=Jul-07 6 2 .4 5 1 .2 1 6 1 4 1 0 .4 9 6 4 8 7 7 1 .1 7 3 7 3 8 3 .7 2 6 2 6 2 R each=R 05, D ate=Jul-07 12 1 2 1 .9 5 8 3 2 1 4 .5 9 3 6 1 .9 4 7 6 7 -1 4 .3 8 7 5 7 2 5 8 .3 0 4 2 Note: T-alpha (R each = R 01, D ate=Jul-07) = 2 .5 7 0 6 , T-alpha (R each = R 05, D ate=Jul-07) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 -119.5083 1 7 7 .9 3 2 4 Unequal 11.00 -1 1 9 .5 0 8 3 2 1 4 .5 9 6 5 Note: T-alp h a (Equal) = 2 .1 1 9 9 , T-alpha (U nequal) = 2 .2 0 1 0 Standard Error 8 8 .9 6 6 2 2 61.9 4 9 6 6 95.0% LCL Difference -3 0 8 .1 0 8 3 -2 5 5 .8 5 6 5 95.0% UCL Difference 69.0 9 1 6 3 1 6 .8 3 9 8 2 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1 .3 4 3 3 0 .1 9 7 9 1 9 No D ifference < 0 -1 .3 4 3 3 0 .0 9 8 9 5 9 No D ifference > 0 -1 .3 4 3 3 0 .9 0 1 0 4 1 No Difference: (R e a c h = R 0 1 , D ate= Ju l-07)-(R each = R 05, D ate=Ju l-07) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .2 4 3 6 8 7 0 .3 6 0 0 1 7 0 .0 0 1 6 7 4 Power (Alpha=.010) 0 .0 8 5 9 9 8 0 .1 3 7 0 5 7 0 .0 0 0 1 8 1 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1.9291 0 .0 7 9 9 0 2 No D ifference < 0 -1.9291 0 .0 3 9 9 5 1 Yes D ifference > 0 -1.9291 0 .9 6 0 0 4 9 No Difference: (R e a c h = R 0 1 , D ate = J u l-0 7 )-(R e a c h = R 0 5 , D ate=Ju l-07) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .4 2 1 1 7 8 0 .5 6 5 1 1 4 0 .0 0 0 2 6 6 Power (Alpha=.010) 0 .1 7 7 3 9 6 0 .2 6 4 7 1 2 0 .0 0 0 0 2 6 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-07) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-07) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 7 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-07) Variance-R atio E qual-V ariance Test 3 1 1 3 6 .0 1 6 3 M odified-Levene E qual-V ariance Test 1 .6 4 9 4 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 2 .7 6 6 2 1 .8 9 3 4 1 1 .2 3 6 8 0 .0 0 0 0 0 0 0 .2 1 7 3 2 7 0 .0 0 5 6 7 2 R eject norm ality 0 .0 5 8 3 0 5 C annot reject norm ality 0 .0 0 3 6 3 1 R eject norm ality R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-07 Reach=R05, Date=Jul-07 Count 6 12 Median 1.9 8.65 95.0% LCL of Median 1.5 7 .3 95.0% UCL of Median 4 .4 302.1 4/10/2013 11:14:50 AM 35 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 01, D ate=Jul-07 R each= R 05, D ate=Jul-07 N um ber Sets of Ties = 2, Mann W Whitney U Sum Ranks 0 21 72 150 Multiplicity Factor = 12 Mean of W 57 114 Std Dev of W 1 0 .6 6 6 0 5 1 0 .6 6 6 0 5 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3 .3 7 5 2 0.000738 Yes -3 .3 7 5 2 0.000369 Yes -3 .3 7 5 2 0.999631 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3 .3 2 8 3 0.00 0 8 7 4 Yes -3 .3 2 8 3 0.00 0 4 3 7 Yes -3.4221 0.99 9 6 8 9 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 2 0 2 0 .6 2 0 2 0 .6 2 0 2 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0001 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:14:50 AM 36 4/10/2013 11:17:47 AM 1153 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-07 12 121.9583 214.593 61.94767 -14.38757 258.3042 Reach=R13, Date=Jul-07 6 8.216666 4.088724 1.669215 3.925814 12.50752 Note: T-alpha (Reach=R05, Date=Jul-07) = 2.2010, T-alpha (Reach=R13, Date =Jul-07) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 113.7417 177.9458 Unequal 11.02 113.7417 214.632 Note: T-alpha (Equal) = 2.1199, T-alpha (Unequal) = 2.2006 Standard Error 88.97291 61.97016 95.0% LCL Difference -74.87247 -22.62961 95.0% UCL Difference 302.3558 250.1129 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.2784 0.219348 No Difference < 0 1.2784 0.890326 No Difference > 0 1.2784 0.109674 No Difference: (Reach=R05, Date=Jul-07)-(Reach= R13, Date=Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.225163 0.002043 0.337052 Power (Alpha=.010) 0.077238 0.000228 0.124444 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.8354 0.093561 No Difference < 0 1.8354 0.953219 No Difference > 0 1.8354 0.046781 Yes Difference: (Reach=R05, Date=Jul-07)-(Reach=R13, Date=Jul-07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.388326 0.000368 0.530490 Power (Alpha=.010) 0.157607 0.000036 0.238720 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-07) Kurtosis Normality (Reach=R05, Date=Jul-07) Omnibus Normality (Reach=R05, Date=Jul-07) Skewness Normality (Reach=R13, Date=Jul-07) Kurtosis Normality (Reach=R13, Date=Jul-07) Omnibus Normality (Reach=R13, Date=Jul-07) Variance-Ratio Equal-Variance Test 2754.5811 Modified-Levene Equal-Variance Test 1.5815 Probability 2.7662 1.8934 11.2368 0.0000 Decision(.050) 0.005672 Reject normality 0.058305 Cannot reject normality 0.003631 Reject normality 1.000000 Cannot reject normality 0.000000 0.226596 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-07 Reach=R13, Date=Jul-07 Count 12 6 Median 8.65 7.85 95.0% LCL of Median 7.3 2.9 95.0% UCL of Median 302.1 14.4 4/10/2013 11:17:47 AM 1154 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-07 Reach=R13, Date=Jul-07 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 49 127 23 44 Multiplicity Factor = 6 Mean of W 114 57 Std Dev of W 10.67157 10.67157 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.2182 0.223152 No -1.2182 0.888424 No -1.2182 0.111576 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.1713 0.241463 No -1.2650 0.897072 No -1.1713 0.120732 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.416667 0.083333 0.416667 Reject H0 if Greater Than 0.6202 0.6202 0.6202 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.4680 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:17:48 AM 1155 4/10/2013 11:15:05 AM 142 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 01, D ate=Jul-08 6 1 .3 2 1 6 6 7 0 .7 9 9 1 3 5 0 .3 2 6 2 4 5 5 0 .4 8 3 0 2 6 2 .1 6 0 3 0 7 R each=R 05, D ate=Jul-08 12 1 2 2 .0 6 4 2 1 7 0 .6 1 0 7 49.2 5 1 0 8 1 3 .6 6 3 2 8 2 3 0 .4 6 5 1 Note: T-alp h a (R each = R 01, D ate=Jul-08) = 2 .5 7 0 6 , T-alpha (R each = R 05, D ate=Ju l-08) = 2 .2 0 1 0 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 -120.7425 1 4 1 .4 6 3 6 Unequal 11.00 -1 2 0 .7 4 2 5 1 7 0 .6 1 2 6 Note: T-alp h a (Equal) = 2 .1 1 9 9 , T-alpha (U nequal) = 2 .2 0 1 0 Standard Error 7 0 .7 3 1 8 2 49.2 5 2 1 6 95.0% LCL Difference -2 7 0 .6 8 7 3 -2 2 9 .1 4 4 6 95.0% UCL Difference 29.2 0 2 2 7 -1 2 .3 4 0 4 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -1 .7 0 7 0 0 .1 0 7 1 4 2 No D ifference < 0 -1 .7 0 7 0 0.053571 No D ifference > 0 -1 .7 0 7 0 0 .9 4 6 4 2 9 No Difference: (R e a c h = R 0 1 , D ate= Ju l-08)-(R each = R 05, D ate=Ju l-08) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .3 6 1 4 4 7 0 .4 9 5 8 1 0 0 .0 0 0 5 1 3 Power (Alpha=.010) 0 .1 4 9 1 3 1 0 .2 2 3 3 3 7 0 .0 0 0 0 4 8 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .4 5 1 5 0 .0 3 2 1 5 7 Yes D ifference < 0 -2 .4 5 1 5 0 .0 1 6 0 7 9 Yes D ifference > 0 -2 .4 5 1 5 0.983921 No Difference: (R e a c h = R 0 1 , D ate = J u l-0 8 )-(R e a c h = R 0 5 , D ate=Ju l-08) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0.608241 0.742761 0 .0 0 0 0 3 8 Power (Alpha=.010) 0 .3 1 4 0 6 1 0 .4 3 0 9 8 4 0 .0 0 0 0 0 3 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-08) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-08) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 8 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-08) Variance-R atio E qual-V ariance Test 4 5 5 7 9 .7 9 9 4 M odified-Levene E qual-V ariance Test 2 .7 5 1 9 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 2 .1 0 0 8 0 .3 1 3 6 4 .5 1 1 9 0 .0 0 0 0 0 0 0 .1 1 6 6 1 2 0 .0 3 5 6 5 4 R eject norm ality 0 .7 5 3 8 2 9 C annot reject norm ality 0 .1 0 4 7 7 4 C annot reject norm ality R eject equal variances C annot reject equal variances Median Statistics Variable Reach=R01, Date=Jul-08 Reach=R05, Date=Jul-08 Count 6 12 Median 1.095 43.115 95.0% LCL of Median 0 .5 5 9 .1 2 95.0% UCL of Median 2 .4 8 3 6 5 .8 4/10/2013 11:15:05 AM 143 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 01, D ate=Jul-08 R each= R 05, D ate=Jul-08 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 21 72 150 Multiplicity F acto r = 0 Mean of W 57 114 Std Dev of W 1 0 .6 7 7 0 8 1 0 .6 7 7 0 8 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.000108 Y es 0.000054 Y es 0.999946 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3 .3 7 1 7 0.000747 Y es -3 .3 7 1 7 0.000374 Y es -3 .3 7 1 7 0.999626 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3 .3 2 4 9 0.000885 Yes -3 .3 2 4 9 0.000442 Yes -3 .4 1 8 5 0.999685 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 1 .0 0 0 0 0 0 1 .0 0 0 0 0 0 0 .0 0 0 0 0 0 Reject H0 if Greater Than 0 .6 2 0 2 0 .6 2 0 2 0 .6 2 0 2 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0001 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFGS_ppb when Reach=R01, Date=Jul- 4/10/2013 11:15:05 AM 144 500 n 400 - Channel_Catfish_F'FOS_ppbvs Groups Reach=R01, Date-Jul-08 Reach=R05, Date=Jul-08 Groups 4/10/2013 11:18:00 AM 1225 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-08 12 122.0642 170.6107 49.25108 13.66328 230.4651 Reach=R13, Date=Jul-08 6 4.061666 2.814594 1.149053 1.107932 7.015401 Note: T-alpha (Reach=R05, Date=Jul-08) = 2.2010, T-alpha (Reach=R13, Date =Jul-08) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 118.0025 141.4717 Unequal 11.01 118.0025 170.6339 Note: T-alpha (Equal) = 2.1199, T-alpha (Unequal) = 2.2007 Standard Error 70.73585 49.26448 95.0% LCL Difference -31.95079 9.586498 95.0% UCL Difference 267.9558 226.4185 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1 .6682 0.114722 No Difference < 0 1.6682 0.942639 No Difference > 0 1.6682 0.057361 No Difference: (Reach=R05, Date=Jul-08)-(Reach= R13, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.347917 0.000585 0.481003 Power (Alpha=.010) 0.141203 0.000055 0.212882 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3953 0.035505 Yes Difference < 0 2.3953 0.982247 No Difference > 0 2.3953 0.017753 Yes Difference: (Reach=R05, Date=Jul-08)-(Reach=R13, Date=Jul-08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.588627 0.000047 0.725597 Power (Alpha=.010) 0.297575 0.000004 0.411974 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-08) Kurtosis Normality (Reach=R05, Date=Jul-08) Omnibus Normality (Reach=R05, Date=Jul-08) Skewness Normality (Reach=R13, Date=Jul-08) Kurtosis Normality (Reach=R13, Date=Jul-08) Omnibus Normality (Reach=R13, Date=Jul-08) Variance-Ratio Equal-Variance Test 3674.3565 Modified-Levene Equal-Variance Test 2.6685 Probability 2.1008 0.3136 4.5119 0.0000 Decision(.050) 0.035654 Reject normality 0.753829 Cannot reject normality 0.104774 Cannot reject normality 1.000000 Cannot reject normality 0.000000 0.121871 Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05, Date=Jul-08 Reach=R13, Date=Jul-08 Count 12 6 Median 43.115 4.33 95.0% LCL of Median 9.12 0.09 95.0% UCL of Median 365.8 7.57 4/10/2013 11:18:00 AM 1226 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-08 Reach=R13, Date=Jul-08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 70 148 2 23 Multiplicity Factor = 0 Mean of W 114 57 Std Dev of W 10.67708 10.67708 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.000431 Yes 0.999785 No 0.000215 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3.1844 0.001451 Yes -3.1844 0.999275 No -3.1844 0.000725 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3.1376 0.001704 Yes -3.2312 0.999384 No -3.1376 0.000852 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.916667 0.000000 0.916667 Reject H0 if Greater Than 0.6202 0.6202 0.6202 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0008 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:00 AM 1227 Norrral Probabilty Plct of Channel_Cstfis:h_=lFQS_ppb when ReachrRI 3, Date- J /, . ........................................................ 1 5 10 25 50 75 SO S6 99 Percent ofValues 4/10/2013 11:15:20 AM 247 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Variable Count Mean Standard Deviation Standard Error 95.0% LCL of Mean 95.0% UCL of Mean R each=R 01, D ate=Jul-09 6 1.87 1 .1 1 7 8 9 1 0 .4 5 6 3 7 7 0 .6 9 6 8 4 5 5 3 .0 4 3 1 5 4 R each=R 05, D ate=Jul-09 12 1 8 2 .8 1 8 3 1 7 1 .4 0 4 5 4 9 .4 8 0 2 2 7 3 .9 1 3 0 9 2 9 1 .7 2 3 6 Note: T-alpha (R each =R 01, D ate=Jul-09) = 2 .5706, T-alpha (R each =R 05, D ate=Jul-09) = 2 .2010 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 -180.9483 1 4 2 .1 2 2 5 Unequal 11.00 -1 8 0 .9 4 8 3 1 7 1 .4 0 8 2 Note: T-alp h a (Equal) = 2 .1 1 9 9 , T-alpha (U nequal) = 2 .2 0 0 9 Standard Error 7 1 .0 6 1 2 5 49.4 8 2 3 3 95.0% LCL Difference -3 3 1 .5 9 1 5 -2 8 9 .8 5 6 95.0% UCL Difference -30.30521 -7 2 .0 4 0 7 2 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -2 .5 4 6 4 0 .0 2 1 5 6 1 Yes D ifference < 0 -2 .5 4 6 4 0 .0 1 0 7 8 0 Yes D ifference > 0 -2 .5 4 6 4 0 .9 8 9 2 2 0 No Difference: (R e a c h = R 0 1 , D ate= Ju l-09)-(R each = R 05, D ate=Ju l-09) T h e random ization test results are based on 100 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .6 6 6 9 8 4 0 .7 8 5 6 9 6 0 .0 0 0 0 2 2 Power (Alpha=.010) 0 .3 8 5 7 9 4 0 .5 0 1 9 1 8 0.000001 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 D ifference <> 0 -3 .6 5 6 8 0 .0 0 3 7 7 4 Yes D ifference < 0 -3 .6 5 6 8 0 .0 0 1 8 8 7 Yes D ifference > 0 -3 .6 5 6 8 0 .9 9 8 1 1 3 No Difference: (R e a c h = R 0 1 , D ate = J u l-0 9 )-(R e a c h = R 0 5 , D ate=Ju l-09) T h e random ization test results are based on 1 0 0 0 M onte C arlo sam ples. Power (Alpha=.050) 0 .9 1 3 5 9 5 0 .9 6 2 0 2 7 0 .0 0 0 0 0 0 Power (Alpha=.010) 0 .6 9 9 8 4 3 0 .8 0 7 5 1 0 0 .0 0 0 0 0 0 Tests of Assumptions Section Assumption Value Skew ness Norm ality (R e a c h = R 01 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 1 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 01 , D ate=Ju l-09) Skew ness Norm ality (R e a c h = R 05 , D ate=Ju l-09) Kurtosis N orm ality (R e a c h = R 0 5 , D a te = J u l-0 9 ) O m nibus Norm ality (R e a c h = R 05 , D ate=Ju l-09) Variance-R atio E qual-V ariance Test 2 3 5 0 9 .6 2 7 5 M odified-Levene E qual-V ariance Test 1 0 .3 9 3 8 Probability 0 .0 0 0 0 Decision(.050) 1 .0 0 0 0 0 0 C annot reject norm ality 0 .8 2 6 4 -1.2111 2 .1 4 9 8 0 .0 0 0 0 0 0 0 .0 0 5 3 0 4 0 .4 0 8 5 8 7 C annot reject norm ality 0 .2 2 5 8 4 4 C annot reject norm ality 0 .3 4 1 3 3 9 C annot reject norm ality R eject equal variances R eject equal variances Median Statistics Variable Reach=R01, Date=Jul-09 Reach=R05, Date=Jul-09 Count 6 12 Median 1.485 136.38 95.0% LCL of Median 0 .9 1 3 .8 7 95.0% UCL of Median 3 .7 7 342.21 4/10/2013 11:15:20 AM 248 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable R each=R 01, D ate=Jul-09 R each= R 05, D ate=Jul-09 N um ber Sets of Ties = 0, Mann W Whitney U Sum Ranks 2 23 70 148 Multiplicity F acto r = 0 Mean of W 57 114 Std Dev of W 1 0 .6 7 7 0 8 1 0 .6 7 7 0 8 Alternative Hypothesis D iff<>0 D iff<0 D iff>0 Exact Probability Prob Reject H0 Level at .050 0.000431 Y es 0.000215 Y es 0.999785 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -3 .1 8 4 4 0.001451 Y es -3 .1 8 4 4 0.000725 Y es -3 .1 8 4 4 0.999275 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -3 .1 3 7 6 0.001704 Yes -3 .1 3 7 6 0.000852 Yes -3 .2 3 1 2 0.999384 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D (1)<>D (2) D (1)<D (2) D (1)>D (2) Dmn Criterion Value 0 .9 1 6 6 6 7 0 .9 1 6 6 6 7 0.000000 Reject H0 if Greater Than 0 .6 2 0 2 0 .6 2 0 2 0 .6 2 0 2 Test Alpha Level 0 .0 5 0 0 .0 2 5 0 .0 2 5 Reject H0 (Test Alpha) Yes Yes No Prob Level 0 .0 0 0 8 Plots Section Dataset Variable Two-Sample Test Report Y.AFall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Normal Probability Plot of Channel_Catfish_PFGS_ppb when Reach=R01, Date=Jul 4/10/2013 11:15:20 AM 249 500 n 400 - Channel_Catfish_PFOS_ppb vs Groups Reach=R01, Date=Jul-09 Reach=R05, Date=Jul-09 Groups 4/10/2013 11:18:13 AM 1294 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard 95.0% LCL 95.0% UCL Variable Count Mean Deviation Error of Mean of Mean Reach=R05, Date=Jul-09 12 182.8183 171.4045 49.48022 73.91309 291.7236 Reach=R13, Date=Jul-09 6 16.26333 6.986372 2.852174 8.931586 23.59508 Note: T-alpha (Reach=R05, Date=Jul-09) = 2.2010, T-alpha (Reach=R13, Date=Jul-09) = 2.5706 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 16 166.555 142.1748 Unequal 11.07 166.555 171.5468 Note: T-alpha (Equal) = 2.1199, T-alpha (Unequal) = 2.1992 Standard Error 71.08739 49.56236 95.0% LCL Difference 15.85647 57.5566 95.0% UCL Difference 317.2535 275.5534 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3430 0.032383 Yes Difference < 0 2.3430 0.983808 No Difference > 0 2.3430 0.016192 Yes Difference: (Reach= R05, Date=Jul-09)-(Reach= R13, Date= Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.595271 0.000049 0.724841 Power (Alpha=.010) 0.318555 0.000004 0.428362 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 3.3605 0.006302 Yes Difference < 0 3.3605 0.996849 No Difference > 0 3.3605 0.003151 Yes Difference: (Reach=R05, Date=Jul-09)-(Reach=R13, Date=Jul-09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.863950 0.000001 0.933258 Power (Alpha=.010) 0.610422 0.000000 0.731074 Tests of Assumptions Section Assumption Value Skewness Normality (Reach=R05, Date=Jul-09) Kurtosis Normality (Reach=R05, Date=Jul-09) Omnibus Normality (Reach=R05, Date=Jul-09) Skewness Normality (Reach=R13, Date=Jul-09) Kurtosis Normality (Reach=R13, Date=Jul-09) Omnibus Normality (Reach=R13, Date=Jul-09) Variance-Ratio Equal-Variance Test 601.9234 Modified-Levene Equal-Variance Test 9.7091 Probability 0.8264 -1.2111 2.1498 0.0000 Decision(.050) 0.408587 Cannot reject normality 0.225844 Cannot reject normality 0.341339 Cannot reject normality 1.000000 Cannot reject normality 0.000001 0.006654 Reject equal variances Reject equal variances Median Statistics Variable Reach=R05, Date=Jul-09 Reach=R13, Date=Jul-09 Count 12 6 Median 136.38 18.36 95.0% LCL of Median 13.87 6.03 95.0% UCL of Median 342.21 23.03 4/10/2013 11:18:13 AM 1295 Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05, Date=Jul-09 Reach=R13, Date=Jul-09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 52 130 20 41 Multiplicity Factor = 0 Mean of W 114 57 Std Dev of W 10.67708 10.67708 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150506 No 0.924747 No 0.075253 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.4985 0.133994 No -1.4985 0.933003 No -1.4985 0.066997 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.4517 0.146583 No -1.5454 0.938871 No -1.4517 0.073291 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.666667 0.166667 0.666667 Reject H0 if Greater Than 0.6202 0.6202 0.6202 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0463 Plots Section Dataset Variable Two-Sample Test Report \...\Fall 2012 Field Data\Data for plots\catfish.NCSS Channel_Catfish_PFOS_ppb 4/10/2013 11:18:13 AM 1296 Nrirrnal Probabi ity Plot of Channel_Catfioh_PFQS_ppb wnen Reach~R13, Date- J 400 - Reach=R05, Date=Jul-09 Reach=R13, Cats=Ju-09 Grouas 5/1/2013 10:43:34 AM 1 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R02 5 2.412 0.8582948 0.3838411 Note: T-alpha (R e ac h =R01) = 2.7764, T-alpha (Reach=R02) = 2.7764 95.0% LCL of Mean -0.2393336 1.346286 95.0% UCL of Mean 4.272933 3.477714 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -0.3952 1.420959 Unequal 5.70 -0.3952 2.009539 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4784 Standard Error 0.8986932 0.8986932 95.0% LCL Difference -2.46759 -2.622549 95.0% UCL Difference 1.67719 1.832149 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.4397 0.671756 No Difference < 0 -0.4397 0.335878 No Difference > 0 -0.4397 0.664122 No Difference: (Reach=R01)-(Reach=R02) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.067560 0.107278 0.020201 Power (Alpha=.010) 0.014989 0.025354 0.003454 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.4397 0.676298 No Difference < 0 -0.4397 0.338149 No Difference > 0 -0.4397 0.661851 No Difference: (Reach=R01)-(Reach=R02) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.065915 0.104768 0.020832 Power (Alpha=.010) 0.014254 0.024026 0.003679 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.4818 0.3660 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.175386 0.561943 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R02 Count 5 5 Median 1.5 2.65 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:34 AM 2 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R02 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 8 23 17 32 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.210318 No 0.789683 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.9400 0.347208 No -0.9400 0.173604 No -0.9400 0.826396 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.8356 0.403395 No -0.8356 0.201698 No -1.0445 0.851865 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:34 AM 3 5/1/2013 10:43:35 AM 4 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R03 5 2.0856 1.438245 0.6432027 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R03) = 2.7764 95.0% LCL of Mean -0.2393336 0.2997831 95.0% UCL of Mean 4.272933 3.871417 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -0.0688 1.638616 Unequal 7.60 -0.0688 2.317353 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3273 Standard Error 1.036352 1.036352 95.0% LCL Difference -2.458632 -2.480741 95.0% UCL Difference 2.321032 2.343141 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.0664 0.948699 No Difference < 0 -0.0664 0.474350 No Difference > 0 -0.0664 0.525650 No Difference: (Reach=R01)-(Reach=R03) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.050397 0.056615 0.044014 Power (Alpha=.010) 0.010110 0.011605 0.008591 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.0664 0.948783 No Difference < 0 -0.0664 0.474392 No Difference > 0 -0.0664 0.525608 No Difference: (Reach=R01)-(Reach=R03) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.050392 0.056585 0.044039 Power (Alpha=.010) 0.010108 0.011591 0.008602 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.5961 0.0287 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.661639 0.869576 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R03 Count 5 5 Median 1.5 2.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:35 AM 5 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R03 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 9 24 16 31 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.273810 No 0.726191 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.7311 0.464702 No -0.7311 0.232351 No -0.7311 0.767649 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.6267 0.530869 No -0.6267 0.265435 No -0.8356 0.798302 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:35 AM 6 5/1/2013 10:43:35 AM 7 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R04 5 18.992 20.75822 9.283358 Note: T-alpha (R e ac h =R01) = 2.7764, T-alpha (Reach=R04) = 2.7764 95.0% LCL of Mean -0.2393336 -6.782733 95.0% UCL of Mean 4.272933 44.76674 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -16.9752 14.7344 Unequal 4.06 -16.9752 20.83759 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7600 Standard Error 9.318854 9.318854 95.0% LCL Difference -38.46452 -42.69522 95.0% UCL Difference 4.514117 8.744818 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8216 0.105993 No Difference < 0 -1.8216 0.052996 No Difference > 0 -1.8216 0.947004 No Difference: (Reach=R01)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.361425 0.508228 0.000437 Power (Alpha=.010) 0.134325 0.211881 0.000049 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8216 0.141530 No Difference < 0 -1.8216 0.070765 No Difference > 0 -1.8216 0.929235 No Difference: (Reach=R01)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.291198 0.448650 0.000634 Power (Alpha=.010) 0.084175 0.148271 0.000094 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 130.5145 2.8256 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000345 0.131277 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R04 Count 5 5 Median 1.5 7.85 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:35 AM 8 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 2 17 23 38 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.015873 Yes 0.984127 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.1934 0.028280 Yes -2.1934 0.014140 Yes -2.1934 0.985860 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.0889 0.036714 Yes -2.0889 0.018357 Yes -2.2978 0.989214 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:35 AM 9 5/1/2013 10:43:35 AM 10 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R05 5 256 188.5815 84.3362 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R05) = 2.7764 95.0% LCL of Mean -0.2393336 21.84515 95.0% UCL of Mean 4.272933 490.1548 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -253.9832 133.3534 Unequal 4.00 -253.9832 188.5902 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7762 Standard Error 84.34013 84.34013 95.0% LCL Difference -448.4719 -488.1318 95.0% UCL Difference -59.49453 -19.83462 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0114 0.016777 Yes Difference < 0 -3.0114 0.008389 Yes Difference > 0 -3.0114 0.991611 No Difference: (Reach=R01)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.751153 0.864075 0.000005 Power (Alpha=.010) 0.430737 0.569299 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0114 0.039486 Yes Difference < 0 -3.0114 0.019743 Yes Difference > 0 -3.0114 0.980257 No Difference: (Reach=R01)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.622418 0.793703 0.000009 Power (Alpha=.010) 0.247675 0.388906 0.000001 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 Probability Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 10771.5125 0.000000 5.7880 0.042786 Reject equal variances Reject equal variances Median Statistics Variable Reach=R01 Reach=R05 Count 5 5 Median 1.5 283 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:35 AM 11 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R05 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:35 AM 12 550 n Channel Cattish F'FOS ppb vs Groups 440 - Q. CL <A tO0=3 _"l 220U 110- 0J------------I Reach=R01 Groups Reach=R05 5/1/2013 10:43:36 AM 13 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R06 5 3.704 0.9185206 0.4107749 Note: T-alpha (R e ac h =R01) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean -0.2393336 2.563506 95.0% UCL of Mean 4.272933 4.844494 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -1.6872 1.439663 Unequal 5.92 -1.6872 2.03599 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4551 Standard Error 0.9105226 0.9105226 95.0% LCL Difference -3.786869 -3.92258 95.0% UCL Difference 0.4124689 0.5481804 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8530 0.101010 No Difference < 0 -1.8530 0.050505 No Difference > 0 -1.8530 0.949495 No Difference: (Reach=R01)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.371728 0.519613 0.000393 Power (Alpha=.010) 0.139778 0.219475 0.000044 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8530 0.113988 No Difference < 0 -1.8530 0.056994 No Difference > 0 -1.8530 0.943006 No Difference: (Reach=R01)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.343864 0.497290 0.000457 Power (Alpha=.010) 0.116888 0.192127 0.000058 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.9133 0.3669 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.214820 0.561492 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R06 Count 5 5 Median 1.5 4.15 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:36 AM 14 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R06 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 5 20 20 35 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.5715 0.116074 No -1.5715 0.058037 No -1.5715 0.941963 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.4667 0.142457 No -1.4667 0.071228 No -1.6762 0.953154 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:36 AM 15 5/1/2013 10:43:36 AM 16 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R07 5 40.32 25.25088 11.29254 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean -0.2393336 8.96689 95.0% UCL of Mean 4.272933 71.67311 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -38.3032 17.90124 Unequal 4.04 -38.3032 25.31617 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7653 Standard Error 11.32174 11.32174 95.0% LCL Difference -64.41117 -69.61072 95.0% UCL Difference -12.19523 -6.995679 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3832 0.009597 Yes Difference < 0 -3.3832 0.004798 Yes Difference > 0 -3.3832 0.995202 No Difference: (Reach=R01)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.841002 0.924236 0.000001 Power (Alpha=.010) 0.544732 0.682824 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3832 0.027267 Yes Difference < 0 -3.3832 0.013633 Yes Difference > 0 -3.3832 0.986367 No Difference: (Reach=R01)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.720487 0.868323 0.000002 Power (Alpha=.010) 0.322014 0.483462 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 193.1219 4.7292 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000159 0.061370 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R07 Count 5 5 Median 1.5 29.2 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:36 AM 17 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:36 AM 18 5/1/2013 10:43:36 AM 19 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R08 5 8.384 8.303127 3.713272 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean -0.2393336 -1.925695 95.0% UCL of Mean 4.272933 18.6937 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.3672 6.010138 Unequal 4.38 -6.3672 8.499619 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6835 Standard Error 3.801145 3.801145 95.0% LCL Difference -15.13266 -16.56756 95.0% UCL Difference 2.398256 3.833156 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.6751 0.132450 No Difference < 0 -1.6751 0.066225 No Difference > 0 -1.6751 0.933775 No Difference: (Reach=R01)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.314769 0.455145 0.000706 Power (Alpha=.010) 0.110792 0.178493 0.000082 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.6751 0.162968 No Difference < 0 -1.6751 0.081484 No Difference > 0 -1.6751 0.918516 No Difference: (Reach=R01)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.262914 0.409487 0.000944 Power (Alpha=.010) 0.075470 0.132947 0.000139 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 20.8815 1.4533 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.012150 0.262437 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R08 Count 5 5 Median 1.5 4.89 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:36 AM 20 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 3 18 22 37 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.027778 Yes 0.972222 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.9845 0.047202 Yes -1.9845 0.023601 Yes -1.9845 0.976399 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.8800 0.060103 No -1.8800 0.030051 Yes -2.0889 0.981643 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:36 AM 21 5/1/2013 10:43:37 AM 22 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R09 5 22.48 13.41611 5.999866 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean -0.2393336 5.8217 95.0% UCL of Mean 4.272933 39.1383 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -20.4632 9.573233 Unequal 4.15 -20.4632 13.5386 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7381 Standard Error 6.054644 6.054644 95.0% LCL Difference -34.42524 -37.04163 95.0% UCL Difference -6.501165 -3.884768 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3798 0.009645 Yes Difference < 0 -3.3798 0.004823 Yes Difference > 0 -3.3798 0.995177 No Difference: (Reach=R01)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.840292 0.923797 0.000001 Power (Alpha=.010) 0.543694 0.681844 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3798 0.026289 Yes Difference < 0 -3.3798 0.013144 Yes Difference > 0 -3.3798 0.986856 No Difference: (Reach=R01)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.726507 0.871182 0.000002 Power (Alpha=.010) 0.330592 0.492238 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 54.5170 2.1703 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001923 0.178923 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R09 Count 5 5 Median 1.5 18.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:37 AM 23 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:37 AM 24 5/1/2013 10:43:37 AM 25 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard1 Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R10 5 12.216 3.566887 1.59516 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R10) = 2.7764 95.0% LCL of Mean -0.2393336 7.787125 95.0% UCL of Mean 4.272933 16.64487 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -10.1992 2.83057 Unequal 5.95 -10.1992 4.003031 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4524 Standard1 Error 1.79021 1.79021 95.0% LCL Difference -14.32743 -14.58952 95.0% UCL Difference -6.070969 -5.80888 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.6972 0.000456 Yes Difference < 0 -5.6972 0.000228 Yes Difference > 0 -5.6972 0.999772 No Difference: (Reach=R01)-(Reach=R10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.998513 0.999773 0.000000 Power (Alpha=.010) 0.968400 0.989729 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.6972 0.001304 Yes Difference < 0 -5.6972 0.000652 Yes Difference > 0 -5.6972 0.999348 No Difference: (Reach=R01)-(Reach=R10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996396 0.999510 0.000000 Power (Alpha=.010) 0.925302 0.974440 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.8535 1.9197 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.219720 0.203291 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R10 Count 5 5 Median 1.5 13.4 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:37 AM 26 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:37 AM 27 5/1/2013 10:43:37 AM 28 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean -0.2393336 8.668879 95.0% UCL of Mean 4.272933 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -13.3012 3.998606 Unequal 4.91 -13.3012 5.654882 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5850 Standard Error 2.52894 2.52894 95.0% LCL Difference -19.13295 -19.83846 95.0% UCL Difference -7.469453 -6.763945 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.2596 0.000765 Yes Difference < 0 -5.2596 0.000382 Yes Difference > 0 -5.2596 0.999618 No Difference: (Reach=R01)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.995344 0.999085 0.000000 Power (Alpha=.010) 0.937310 0.975710 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.2596 0.003484 Yes Difference < 0 -5.2596 0.001742 Yes Difference > 0 -5.2596 0.998258 No Difference: (Reach=R01)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.983613 0.997173 0.000000 Power (Alpha=.010) 0.807337 0.916356 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 8.6856 4.0097 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.059556 0.080215 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R11 Count 5 5 Median 1.5 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:37 AM 29 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:37 AM 30 5/1/2013 10:43:37 AM 31 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean -0.2393336 4.463322 95.0% UCL of Mean 4.272933 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.6972 2.740534 Unequal 6.09 -6.6972 3.875701 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4384 Standard Error 1.733266 1.733266 95.0% LCL Difference -10.69412 -10.92353 95.0% UCL Difference -2.700281 -2.47087 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.8639 0.004782 Yes Difference < 0 -3.8639 0.002391 Yes Difference > 0 -3.8639 0.997609 No Difference: (Reach=R01)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.920944 0.969035 0.000000 Power (Alpha=.010) 0.684572 0.805821 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.8639 0.008093 Yes Difference < 0 -3.8639 0.004047 Yes Difference > 0 -3.8639 0.995953 No Difference: (Reach=R01)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.894746 0.959831 0.000000 Power (Alpha=.010) 0.596842 0.744207 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.5497 1.0889 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.247389 0.327219 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R12 Count 5 5 Median 1.5 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:37 AM 32 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 2 17 23 38 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.015873 Yes 0.984127 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.1934 0.028280 Yes -2.1934 0.014140 Yes -2.1934 0.985860 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.0889 0.036714 Yes -2.0889 0.018357 Yes -2.2978 0.989214 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:37 AM 33 5/1/2013 10:43:38 AM 34 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard1 Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R13) = 2.7764 95.0% LCL of Mean -0.2393336 7.741103 95.0% UCL of Mean 4.272933 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -7.7552 1.728706 Unequal 7.91 -7.7552 2.444759 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3104 Standard1 Error 1.09333 1.09333 95.0% LCL Difference -10.27642 -10.28125 95.0% UCL Difference -5.233978 -5.229151 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.0932 0.000103 Yes Difference < 0 -7.0932 0.000051 Yes Difference > 0 -7.0932 0.999949 No Difference: (Reach=R01)-(Reach=R13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999983 0.999999 0.000000 Power (Alpha=.010) 0.998025 0.999667 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.0932 0.000108 Yes Difference < 0 -7.0932 0.000054 Yes Difference > 0 -7.0932 0.999946 No Difference: (Reach=R01)-(Reach=R13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999982 0.999999 0.000000 Power (Alpha=.010) 0.997910 0.999647 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.2341 0.0260 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.843392 0.875871 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R13 Count 5 5 Median 1.5 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:38 AM 35 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:38 AM 36 5/1/2013 10:43:38 AM 37 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean -0.2393336 4.72547 95.0% UCL of Mean 4.272933 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -7.0992 2.81113 Unequal 5.97 -7.0992 3.975538 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4494 Standard Error 1.777915 1.777915 95.0% LCL Difference -11.19908 -11.45406 95.0% UCL Difference -2.999321 -2.744339 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.9930 0.003989 Yes Difference < 0 -3.9930 0.001994 Yes Difference > 0 -3.9930 0.998006 No Difference: (Reach=R01)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.935995 0.976303 0.000000 Power (Alpha=.010) 0.718650 0.832994 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.9930 0.007238 Yes Difference < 0 -3.9930 0.003619 Yes Difference > 0 -3.9930 0.996381 No Difference: (Reach=R01)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.910377 0.967847 0.000000 Power (Alpha=.010) 0.623665 0.768742 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.7871 1.5150 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.225362 0.253332 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R14 Count 5 5 Median 1.5 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:38 AM 38 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:38 AM 39 5/1/2013 10:43:38 AM 40 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard1 Variable Count Mean Deviation Error Reach=R01 5 2.0168 1.817024 0.8125979 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R01) = 2.7764, T-alpha (Reach=R15) = 2.7764 95.0% LCL of Mean -0.2393336 5.414244 95.0% UCL of Mean 4.272933 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -5.9292 1.931205 Unequal 7.90 -5.9292 2.731137 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3113 Standard1 Error 1.221401 1.221401 95.0% LCL Difference -8.745757 -8.752226 95.0% UCL Difference -3.112643 -3.106174 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.8544 0.001265 Yes Difference < 0 -4.8544 0.000632 Yes Difference > 0 -4.8544 0.999368 No Difference: (Reach=R01)-(Reach=R15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.987985 0.997062 0.000000 Power (Alpha=.010) 0.890574 0.950746 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.8544 0.001313 Yes Difference < 0 -4.8544 0.000656 Yes Difference > 0 -4.8544 0.999344 No Difference: (Reach=R01)-(Reach=R15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.987706 0.997003 0.000000 Power (Alpha=.010) 0.888035 0.949549 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R01) Kurtosis Normality (Reach=R01) Omnibus Normality (Reach=R01) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.2593 0.3109 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.828626 0.592401 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R01 Reach=R15 Count 5 5 Median 1.5 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:38 AM 41 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R01 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:38 AM 42 5/1/2013 10:43:39 AM 43 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R03 5 2.0856 1.438245 0.6432027 Note: T-alpha (R e ac h =R02) = 2.7764, T-alpha (Reach=R03) = 2.7764 95.0% LCL of Mean 1.346286 0.2997831 95.0% UCL of Mean 3.477714 3.871417 Confidence-Limits of Difference Section Variance Assumption DF Equal 8 Unequal 6.53 Note: T-alpha (Equal) = 2.3060, Mean Standard Difference Deviation 0.3264 1.184318 0.3264 1.674879 T-alpha (Unequal) = 2.3997 Standard Error 0.7490285 0.7490285 95.0% LCL Difference -1.400863 -1.471043 95.0% UCL Difference 2.053663 2.123843 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.4358 0.674527 No Difference < 0 0.4358 0.662737 No Difference > 0 0.4358 0.337263 No Difference: (Reach=R02)-(Reach=R03) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.067241 0.020381 0.106604 Power (Alpha=.010) 0.014896 0.003489 0.025156 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.4358 0.677050 No Difference < 0 0.4358 0.661475 No Difference > 0 0.4358 0.338525 No Difference: (Reach=R02)-(Reach=R03) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.066317 0.020736 0.105204 Power (Alpha=.010) 0.014475 0.003617 0.024401 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.8080 0.3374 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.341335 0.577307 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R03 Count 5 5 Median 2.65 2.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:39 AM 44 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R03 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 16 31 9 24 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.726191 No 0.273810 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.7311 0.464702 No 0.7311 0.767649 No 0.7311 0.232351 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.6267 0.530869 No 0.8356 0.798302 No 0.6267 0.265435 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:39 AM 45 4.5 -, Channel_Catfish_PFOS_ppb vs Groups 5/1/2013 10:43:39 AM 46 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R04 5 18.992 20.75822 9.283358 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R04) = 2.7764 95.0% LCL of Mean 1.346286 -6.782733 95.0% UCL of Mean 3.477714 44.76674 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -16.58 14.69082 Unequal 4.01 -16.58 20.77596 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7727 Standard Error 9.29129 9.29129 95.0% LCL Difference -38.00575 -42.34212 95.0% UCL Difference 4.845753 9.182126 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.7845 0.112183 No Difference < 0 -1.7845 0.056092 No Difference > 0 -1.7845 0.943908 No Difference: (Reach=R02)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.349371 0.494758 0.000494 Power (Alpha=.010) 0.128065 0.203099 0.000056 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.7845 0.148663 No Difference < 0 -1.7845 0.074331 No Difference > 0 -1.7845 0.925669 No Difference: (Reach=R02)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.280316 0.435349 0.000716 Power (Alpha=.010) 0.079873 0.141434 0.000107 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 584.9345 3.0243 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000017 0.120217 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R04 Count 5 5 Median 2.65 7.85 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:39 AM 47 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 3 18 22 37 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.027778 Yes 0.972222 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.9845 0.047202 Yes -1.9845 0.023601 Yes -1.9845 0.976399 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.8800 0.060103 No -1.8800 0.030051 Yes -2.0889 0.981643 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:39 AM 48 5/1/2013 10:43:39 AM 49 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R05 5 256 188.5815 84.3362 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R05) = 2.7764 95.0% LCL of Mean 1.346286 21.84515 95.0% UCL of Mean 3.477714 490.1548 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -253.588 133.3486 Unequal 4.00 -253.588 188.5835 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7764 Standard Error 84.33708 84.33708 95.0% LCL Difference -448.0697 -487.7415 95.0% UCL Difference -59.10634 -19.43455 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0068 0.016895 Yes Difference < 0 -3.0068 0.008447 Yes Difference > 0 -3.0068 0.991553 No Difference: (Reach=R02)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.749898 0.863172 0.000005 Power (Alpha=.010) 0.429344 0.567838 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0068 0.039672 Yes Difference < 0 -3.0068 0.019836 Yes Difference > 0 -3.0068 0.980164 No Difference: (Reach=R02)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.621135 0.792647 0.000010 Power (Alpha=.010) 0.246811 0.387761 0.000001 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 Probability Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 48275.3200 0.000000 5.8265 0.042256 Reject equal variances Reject equal variances Median Statistics Variable Reach=R02 Reach=R05 Count 5 5 Median 2.65 283 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:39 AM 50 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R05 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:39 AM 51 5/1/2013 10:43:39 AM 52 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R06 5 3.704 0.9185206 0.4107749 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean 1.346286 2.563506 95.0% UCL of Mean 3.477714 4.844494 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -1.292 0.8889179 Unequal 7.96 -1.292 1.25712 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3078 Standard Error 0.562201 0.562201 95.0% LCL Difference -2.588438 -2.589473 95.0% UCL Difference 0.004437904 0.005473302 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.2981 0.050620 No Difference < 0 -2.2981 0.025310 Yes Difference > 0 -2.2981 0.974690 No Difference: (Reach=R02)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.523989 0.674846 0.000081 Power (Alpha=.010) 0.232533 0.341797 0.000008 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.2981 0.050764 No Difference < 0 -2.2981 0.025382 Yes Difference > 0 -2.2981 0.974618 No Difference: (Reach=R02)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.523486 0.674510 0.000081 Power (Alpha=.010) 0.231950 0.341190 0.000008 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.1453 0.0044 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.898586 0.948659 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R06 Count 5 5 Median 2.65 4.15 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:39 AM 53 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R06 Number Sets of Ties = 2, Mann W Whitney U Sum Ranks 3.5 18.5 21.5 36.5 Multiplicity Factor = 12 Mean of W 27.5 27.5 Std Dev of W 4.758034 4.758034 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.8915 0.058553 No -1.8915 0.029276 Yes -1.8915 0.970724 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.7865 0.074026 No -1.7865 0.037013 Yes -1.9966 0.977067 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:40 AM 54 5/1/2013 10:43:40 AM 55 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R07 5 40.32 25.25088 11.29254 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 1.346286 8.96689 95.0% UCL of Mean 3.477714 71.67311 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -37.908 17.86538 Unequal 4.01 -37.908 25.26546 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7739 Standard Error 11.29906 11.29906 95.0% LCL Difference -63.96368 -69.25072 95.0% UCL Difference -11.85232 -6.565285 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3550 0.010006 Yes Difference < 0 -3.3550 0.005003 Yes Difference > 0 -3.3550 0.994997 No Difference: (Reach=R02)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.835052 0.920546 0.000001 Power (Alpha=.010) 0.536126 0.674668 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3550 0.028337 Yes Difference < 0 -3.3550 0.014169 Yes Difference > 0 -3.3550 0.985831 No Difference: (Reach=R02)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.711543 0.862420 0.000002 Power (Alpha=.010) 0.313524 0.473510 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 865.5259 4.9836 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000008 0.056086 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R07 Count 5 5 Median 2.65 29.2 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:40 AM 56 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:40 AM 57 5/1/2013 10:43:40 AM 58 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R08 5 8.384 8.303127 3.713272 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 1.346286 -1.925695 95.0% UCL of Mean 3.477714 18.6937 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -5.972 5.902483 Unequal 4.09 -5.972 8.347371 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7537 Standard Error 3.733058 3.733058 95.0% LCL Difference -14.58045 -16.25167 95.0% UCL Difference 2.636446 4.30767 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.5998 0.148319 No Difference < 0 -1.5998 0.074160 No Difference > 0 -1.5998 0.925840 No Difference: (Reach=R02)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.291841 0.428093 0.000897 Power (Alpha=.010) 0.099898 0.162673 0.000107 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.5998 0.183418 No Difference < 0 -1.5998 0.091709 No Difference > 0 -1.5998 0.908291 No Difference: (Reach=R02)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.237677 0.378751 0.001227 Power (Alpha=.010) 0.065151 0.116790 0.000187 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 93.5859 1.8453 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000666 0.211393 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R08 Count 5 5 Median 2.65 4.89 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:40 AM 59 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 3 18 22 37 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.027778 Yes 0.972222 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.9845 0.047202 Yes -1.9845 0.023601 Yes -1.9845 0.976399 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.8800 0.060103 No -1.8800 0.030051 Yes -2.0889 0.981643 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:40 AM 60 5/1/2013 10:43:40 AM 61 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R09 5 22.48 13.41611 5.999866 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 1.346286 5.8217 95.0% UCL of Mean 3.477714 39.1383 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -20.068 9.506016 Unequal 4.03 -20.068 13.44354 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7676 Standard Error 6.012132 6.012132 95.0% LCL Difference -33.932 -36.70704 95.0% UCL Difference -6.203998 -3.428958 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3379 0.010263 Yes Difference < 0 -3.3379 0.005131 Yes Difference > 0 -3.3379 0.994869 No Difference: (Reach=R02)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.831383 0.918248 0.000001 Power (Alpha=.010) 0.530909 0.669691 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3379 0.028537 Yes Difference < 0 -3.3379 0.014269 Yes Difference > 0 -3.3379 0.985731 No Difference: (Reach=R02)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.708995 0.860272 0.000002 Power (Alpha=.010) 0.312194 0.471482 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 244.3319 2.4775 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000099 0.154131 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R09 Count 5 5 Median 2.65 18.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:40 AM 62 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:40 AM 63 40 - 10- 0 Reach=R02 Reach=R09 Groups 5/1/2013 10:43:41 AM 64 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R10 5 12.216 3.566887 1.59516 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 1.346286 7.787125 95.0% UCL of Mean 3.477714 16.64487 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -9.804 2.594162 Unequal 4.46 -9.804 3.668699 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6667 Standard Error 1.640692 1.640692 95.0% LCL Difference -13.58744 -14.17929 95.0% UCL Difference -6.020557 -5.428715 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.9755 0.000332 Yes Difference < 0 -5.9755 0.000166 Yes Difference > 0 -5.9755 0.999834 No Difference: (Reach=R 02)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999325 0.999913 0.000000 Power (Alpha=.010) 0.980469 0.994365 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.9755 0.002763 Yes Difference < 0 -5.9755 0.001382 Yes Difference > 0 -5.9755 0.998618 No Difference: (Reach=R 02)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.994137 0.999394 0.000000 Power (Alpha=.010) 0.865577 0.952985 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 17.2705 4.1038 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.017318 0.077362 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R10 Count 5 5 Median 2.65 13.4 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:41 AM 65 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:41 AM 66 5/1/2013 10:43:41 AM 67 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 1.346286 8.668879 95.0% UCL of Mean 3.477714 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -12.906 3.834892 Unequal 4.21 -12.906 5.423356 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7238 Standard Error 2.425399 2.425399 95.0% LCL Difference -18.49898 -19.51225 95.0% UCL Difference -7.313021 -6.299752 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.3212 0.000710 Yes Difference < 0 -5.3212 0.000355 Yes Difference > 0 -5.3212 0.999645 No Difference: (Reach=R02)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996006 0.999241 0.000000 Power (Alpha=.010) 0.942772 0.978349 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.3212 0.005207 Yes Difference < 0 -5.3212 0.002604 Yes Difference > 0 -5.3212 0.997396 No Difference: (Reach=R02)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.976182 0.996103 0.000000 Power (Alpha=.010) 0.741055 0.881293 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 38.9267 6.3755 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.003701 0.035533 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R02 Reach=R11 Count 5 5 Median 2.65 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:41 AM 68 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:41 AM 69 5/1/2013 10:43:41 AM 70 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 1.346286 4.463322 95.0% UCL of Mean 3.477714 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.302 2.495611 Unequal 4.50 -6.302 3.529327 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6587 Standard Error 1.578363 1.578363 95.0% LCL Difference -9.941711 -10.49845 95.0% UCL Difference -2.662288 -2.105554 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.9927 0.003990 Yes Difference < 0 -3.9927 0.001995 Yes Difference > 0 -3.9927 0.998005 No Difference: (Reach=R02)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.935968 0.976290 0.000000 Power (Alpha=.010) 0.718587 0.832944 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.9927 0.012844 Yes Difference < 0 -3.9927 0.006422 Yes Difference > 0 -3.9927 0.993578 No Difference: (Reach=R02)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.867646 0.953097 0.000000 Power (Alpha=.010) 0.501951 0.674766 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 15.9087 2.1868 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.020159 0.177459 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R12 Count 5 5 Median 2.65 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:41 AM 71 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:41 AM 72 5/1/2013 10:43:42 AM 73 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (R e ac h =R02) = 2.7764, T-alpha (Reach=R13) = 2.7764 95.0% LCL of Mean 1.346286 7.741103 95.0% UCL of Mean 3.477714 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -7.36 1.306128 Unequal 6.05 -7.36 1.847144 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4422 Standard Error 0.8260678 0.8260678 95.0% LCL Difference -9.264915 -9.377461 95.0% UCL Difference -5.455084 -5.342538 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -8.9097 0.000020 Yes Difference < 0 -8.9097 0.000010 Yes Difference > 0 -8.9097 0.999990 No Difference: (Reach=R 02)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 1.000000 1.000000 0.000000 Power (Alpha=.010) 0.999986 0.999999 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -8.9097 0.000107 Yes Difference < 0 -8.9097 0.000053 Yes Difference > 0 -8.9097 0.999947 No Difference: (Reach=R 02)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 1.000000 1.000000 0.000000 Power (Alpha=.010) 0.999783 0.999987 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.6316 1.0526 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.239441 0.334927 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R13 Count 5 5 Median 2.65 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:42 AM 74 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:42 AM 75 5/1/2013 10:43:42 AM 76 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R02) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 1.346286 4.72547 95.0% UCL of Mean 3.477714 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.704 2.572936 Unequal 4.47 -6.704 3.638681 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6651 Standard Error 1.627268 1.627268 95.0% LCL Difference -10.45649 -11.04079 95.0% UCL Difference -2.951514 -2.367207 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.1198 0.003345 Yes Difference < 0 -4.1198 0.001673 Yes Difference > 0 -4.1198 0.998327 No Difference: (Reach=R02)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.948499 0.981985 0.000000 Power (Alpha=.010) 0.750228 0.857134 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.1198 0.011615 Yes Difference < 0 -4.1198 0.005808 Yes Difference > 0 -4.1198 0.994192 No Difference: (Reach=R02)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.885193 0.961840 0.000000 Power (Alpha=.010) 0.528067 0.700782 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 16.9728 3.2854 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.017886 0.107469 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R14 Count 5 5 Median 2.65 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:42 AM 77 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:42 AM 78 5/1/2013 10:43:42 AM 79 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R02 5 2.412 0.8582948 0.3838411 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (R e ac h =R02) = 2.7764, T-alpha (Reach=R15) = 2.7764 95.0% LCL of Mean 1.346286 5.414244 95.0% UCL of Mean 3.477714 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -5.534 1.564321 Unequal 5.37 -5.534 2.212284 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5176 Standard Error 0.9893634 0.9893634 95.0% LCL Difference -7.815476 -8.024868 95.0% UCL Difference -3.252524 -3.043132 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.5935 0.000514 Yes Difference < 0 -5.5935 0.000257 Yes Difference > 0 -5.5935 0.999743 No Difference: (Reach=R 02)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.998029 0.999680 0.000000 Power (Alpha=.010) 0.962535 0.987289 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.5935 0.001998 Yes Difference < 0 -5.5935 0.000999 Yes Difference > 0 -5.5935 0.999001 No Difference: (Reach=R 02)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.993647 0.999103 0.000000 Power (Alpha=.010) 0.887855 0.958578 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R02) Kurtosis Normality (Reach=R02) Omnibus Normality (Reach=R02) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.6437 2.2151 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.122295 0.174986 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R02 Reach=R15 Count 5 5 Median 2.65 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:42 AM 80 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R02 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:42 AM 81 5/1/2013 10:43:43 AM 82 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R04 5 18.992 20.75822 9.283358 Note: T-alpha (R e ac h =R03) = 2.7764, T-alpha (Reach=R04) = 2.7764 95.0% LCL of Mean 0.2997831 -6.782733 95.0% UCL of Mean 3.871417 44.76674 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -16.9064 14.71347 Unequal 4.04 -16.9064 20.80799 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7661 Standard Error 9.305614 9.305614 95.0% LCL Difference -38.36518 -42.64632 95.0% UCL Difference 4.552383 8.833517 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8168 0.106775 No Difference < 0 -1.8168 0.053387 No Difference > 0 -1.8168 0.946613 No Difference: (Reach=R03)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.359858 0.506487 0.000444 Power (Alpha=.010) 0.133504 0.210733 0.000050 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.8168 0.142728 No Difference < 0 -1.8168 0.071364 No Difference > 0 -1.8168 0.928636 No Difference: (Reach=R03)-(Reach=R04) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.289250 0.446440 0.000646 Power (Alpha=.010) 0.083308 0.146952 0.000096 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 208.3121 2.8933 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000137 0.127367 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R04 Count 5 5 Median 2.01 7.85 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:43 AM 83 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R04 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 1 16 24 39 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.007937 Yes 0.992063 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.4023 0.016294 Yes -2.4023 0.008147 Yes -2.4023 0.991853 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.2978 0.021572 Yes -2.2978 0.010786 Yes -2.5067 0.993907 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:43 AM 84 5/1/2013 10:43:43 AM 85 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R05 5 256 188.5815 84.3362 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R05) = 2.7764 95.0% LCL of Mean 0.2997831 21.84515 95.0% UCL of Mean 3.871417 490.1548 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -253.9144 133.3511 Unequal 4.00 -253.9144 188.587 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7763 Standard Error 84.33866 84.33866 95.0% LCL Difference -448.3997 -488.0653 95.0% UCL Difference -59.4291 -19.76348 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0107 0.016797 Yes Difference < 0 -3.0107 0.008398 Yes Difference > 0 -3.0107 0.991602 No Difference: (Reach=R03)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.750944 0.863925 0.000005 Power (Alpha=.010) 0.430504 0.569055 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.0107 0.039519 Yes Difference < 0 -3.0107 0.019760 Yes Difference > 0 -3.0107 0.980240 No Difference: (Reach=R03)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.622192 0.793520 0.000009 Power (Alpha=.010) 0.247518 0.388701 0.000001 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 Probability Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 17192.2406 0.000000 5.8004 0.042614 Reject equal variances Reject equal variances Median Statistics Variable Reach=R03 Reach=R05 Count 5 5 Median 2.01 283 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:43 AM 86 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R05 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:43 AM 87 5/1/2013 10:43:43 AM 88 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R06 5 3.704 0.9185206 0.4107749 Note: T-alpha (R e ac h =R03) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean 0.2997831 2.563506 95.0% UCL of Mean 3.871417 4.844494 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -1.6184 1.206696 Unequal 6.80 -1.6184 1.706525 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3790 Standard Error 0.7631813 0.7631813 95.0% LCL Difference -3.378299 -3.433992 95.0% UCL Difference 0.1414992 0.1971916 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.1206 0.066763 No Difference < 0 -2.1206 0.033382 Yes Difference > 0 -2.1206 0.966618 No Difference: (Reach=R03)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.462472 0.614964 0.000155 Power (Alpha=.010) 0.192129 0.289997 0.000016 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.1206 0.072828 No Difference < 0 -2.1206 0.036414 Yes Difference > 0 -2.1206 0.963586 No Difference: (Reach=R03)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.444965 0.602349 0.000170 Power (Alpha=.010) 0.174676 0.270674 0.000019 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.4518 0.3210 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.406309 0.586531 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R06 Count 5 5 Median 2.01 4.15 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:43 AM 89 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R06 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 5 20 20 35 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.5715 0.116074 No -1.5715 0.058037 No -1.5715 0.941963 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.4667 0.142457 No -1.4667 0.071228 No -1.6762 0.953154 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:43 AM 90 5/1/2013 10:43:43 AM 91 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R07 5 40.32 25.25088 11.29254 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 0.2997831 8.96689 95.0% UCL of Mean 3.871417 71.67311 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -38.2344 17.88401 Unequal 4.03 -38.2344 25.29181 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7694 Standard Error 11.31084 11.31084 95.0% LCL Difference -64.31725 -69.55865 95.0% UCL Difference -12.15156 -6.910153 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3803 0.009637 Yes Difference < 0 -3.3803 0.004818 Yes Difference > 0 -3.3803 0.995182 No Difference: (Reach=R03)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.840413 0.923872 0.000001 Power (Alpha=.010) 0.543871 0.682011 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3803 0.027501 Yes Difference < 0 -3.3803 0.013751 Yes Difference > 0 -3.3803 0.986249 No Difference: (Reach=R03)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.718774 0.867324 0.000002 Power (Alpha=.010) 0.320066 0.481319 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 308.2389 4.8177 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000063 0.059463 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R07 Count 5 5 Median 2.01 29.2 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:44 AM 92 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:44 AM 93 5/1/2013 10:43:44 AM 94 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R08 5 8.384 8.303127 3.713272 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 0.2997831 -1.925695 95.0% UCL of Mean 3.871417 18.6937 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.2984 5.958627 Unequal 4.24 -6.2984 8.426771 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7156 Standard Error 3.768567 3.768567 95.0% LCL Difference -14.98873 -16.53229 95.0% UCL Difference 2.391931 3.935494 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.6713 0.133207 No Difference < 0 -1.6713 0.066603 No Difference > 0 -1.6713 0.933397 No Difference: (Reach=R03)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.313601 0.453783 0.000714 Power (Alpha=.010) 0.110227 0.177678 0.000083 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.6713 0.165930 No Difference < 0 -1.6713 0.082965 No Difference > 0 -1.6713 0.917035 No Difference: (Reach=R03)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.258519 0.405004 0.000973 Power (Alpha=.010) 0.073186 0.129659 0.000145 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 33.3287 1.5891 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.004993 0.242979 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R08 Count 5 5 Median 2.01 4.89 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:44 AM 95 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 4 19 21 36 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.047619 Yes 0.952381 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.7756 0.075800 No -1.7756 0.037900 Yes -1.7756 0.962100 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.6711 0.094693 No -1.6711 0.047346 Yes -1.8800 0.969949 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:44 AM 96 5/1/2013 10:43:45 AM 97 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R09 5 22.48 13.41611 5.999866 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 0.2997831 5.8217 95.0% UCL of Mean 3.871417 39.1383 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -20.3944 9.540978 Unequal 4.09 -20.3944 13.49298 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7520 Standard Error 6.034245 6.034245 95.0% LCL Difference -34.30939 -37.00075 95.0% UCL Difference -6.479407 -3.788049 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3798 0.009645 Yes Difference < 0 -3.3798 0.004822 Yes Difference > 0 -3.3798 0.995178 No Difference: (Reach=R03)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.840297 0.923801 0.000001 Power (Alpha=.010) 0.543701 0.681851 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3798 0.026833 Yes Difference < 0 -3.3798 0.013417 Yes Difference > 0 -3.3798 0.986583 No Difference: (Reach=R03)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.723007 0.869430 0.000002 Power (Alpha=.010) 0.325805 0.487287 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 87.0137 2.2769 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000769 0.169748 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R09 Count 5 5 Median 2.01 18.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:45 AM 98 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:45 AM 99 5/1/2013 10:43:45 AM 100 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R10 5 12.216 3.566887 1.59516 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 0.2997831 7.787125 95.0% UCL of Mean 3.871417 16.64487 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -10.1304 2.719488 Unequal 5.27 -10.1304 3.845937 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5319 Standard Error 1.719955 1.719955 95.0% LCL Difference -14.09662 -14.48507 95.0% UCL Difference -6.164176 -5.775732 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.8899 0.000366 Yes Difference < 0 -5.8899 0.000183 Yes Difference > 0 -5.8899 0.999817 No Difference: (Reach=R 03)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999135 0.999882 0.000000 Power (Alpha=.010) 0.977268 0.993192 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.8899 0.001679 Yes Difference < 0 -5.8899 0.000839 Yes Difference > 0 -5.8899 0.999161 No Difference: (Reach=R 03)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996431 0.999592 0.000000 Power (Alpha=.010) 0.913492 0.971411 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 6.1505 2.6958 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.106407 0.139237 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R10 Count 5 5 Median 2.01 13.4 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:45 AM 101 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:45 AM 102 5/1/2013 10:43:45 AM 103 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 0.2997831 8.668879 95.0% UCL of Mean 3.871417 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -13.2324 3.920757 Unequal 4.57 -13.2324 5.544787 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6443 Standard Error 2.479704 2.479704 95.0% LCL Difference -18.95061 -19.78941 95.0% UCL Difference -7.514192 -6.675392 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.3363 0.000697 Yes Difference < 0 -5.3363 0.000349 Yes Difference > 0 -5.3363 0.999651 No Difference: (Reach=R03)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.996154 0.999276 0.000000 Power (Alpha=.010) 0.944051 0.978957 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.3363 0.004038 Yes Difference < 0 -5.3363 0.002019 Yes Difference > 0 -5.3363 0.997981 No Difference: (Reach=R03)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.982268 0.997116 0.000000 Power (Alpha=.010) 0.787385 0.907209 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 13.8629 4.9418 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.025943 0.056914 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R11 Count 5 5 Median 2.01 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:45 AM 104 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:45 AM 105 5/1/2013 10:43:45 AM 106 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 0.2997831 4.463322 95.0% UCL of Mean 3.871417 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.6284 2.625645 Unequal 5.37 -6.6284 3.713223 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5183 Standard Error 1.660604 1.660604 95.0% LCL Difference -10.45776 -10.81029 95.0% UCL Difference -2.79904 -2.446514 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.9916 0.003997 Yes Difference < 0 -3.9916 0.001998 Yes Difference > 0 -3.9916 0.998002 No Difference: (Reach=R03)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.935841 0.976231 0.000000 Power (Alpha=.010) 0.718282 0.832707 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.9916 0.009011 Yes Difference < 0 -3.9916 0.004506 Yes Difference > 0 -3.9916 0.995494 No Difference: (Reach=R03)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.896684 0.963196 0.000000 Power (Alpha=.010) 0.580514 0.737035 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.6656 1.4621 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.121538 0.261118 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R12 Count 5 5 Median 2.01 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:45 AM 107 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:46 AM 108 5/1/2013 10:43:46 AM 109 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 0.2997831 7.741103 95.0% UCL of Mean 3.871417 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -7.6864 1.5401 Unequal 7.87 -7.6864 2.178031 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3126 Standard Error 0.974045 0.974045 95.0% LCL Difference -9.932551 -9.938963 95.0% UCL Difference -5.440248 -5.433836 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.8912 0.000048 Yes Difference < 0 -7.8912 0.000024 Yes Difference > 0 -7.8912 0.999976 No Difference: (Reach=R 03)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999999 1.000000 0.000000 Power (Alpha=.010) 0.999730 0.999971 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.8912 0.000053 Yes Difference < 0 -7.8912 0.000026 Yes Difference > 0 -7.8912 0.999974 No Difference: (Reach=R 03)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999999 1.000000 0.000000 Power (Alpha=.010) 0.999698 0.999967 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.2933 0.1650 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.809200 0.695238 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R13 Count 5 5 Median 2.01 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:46 AM 110 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:46 AM 111 5/1/2013 10:43:46 AM 112 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 0.2997831 4.72547 95.0% UCL of Mean 3.871417 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -7.0304 2.699248 Unequal 5.29 -7.0304 3.817313 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5290 Standard Error 1.707154 1.707154 95.0% LCL Difference -10.9671 -11.3478 95.0% UCL Difference -3.093695 -2.713001 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.1182 0.003353 Yes Difference < 0 -4.1182 0.001676 Yes Difference > 0 -4.1182 0.998324 No Difference: (Reach=R03)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.948355 0.981922 0.000000 Power (Alpha=.010) 0.749844 0.856847 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.1182 0.008160 Yes Difference < 0 -4.1182 0.004080 Yes Difference > 0 -4.1182 0.995920 No Difference: (Reach=R03)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.911286 0.970339 0.000000 Power (Alpha=.010) 0.605559 0.760276 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 6.0445 2.1296 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.109465 0.182602 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R14 Count 5 5 Median 2.01 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:46 AM 113 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:46 AM 114 5/1/2013 10:43:46 AM 115 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R03 5 2.0856 1.438245 0.6432027 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R03) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 0.2997831 5.414244 95.0% UCL of Mean 3.871417 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -5.8604 1.764381 Unequal 7.19 -5.8604 2.495211 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3520 Standard Error 1.115892 1.115892 95.0% LCL Difference -8.433652 -8.484959 95.0% UCL Difference -3.287148 -3.235842 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.2518 0.000772 Yes Difference < 0 -5.2518 0.000386 Yes Difference > 0 -5.2518 0.999614 No Difference: (Reach=R 03)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.995253 0.999063 0.000000 Power (Alpha=.010) 0.936587 0.975356 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.2518 0.001087 Yes Difference < 0 -5.2518 0.000544 Yes Difference > 0 -5.2518 0.999456 No Difference: (Reach=R 03)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.993971 0.998849 0.000000 Power (Alpha=.010) 0.919431 0.968342 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R03) Kurtosis Normality (Reach=R03) Omnibus Normality (Reach=R03) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.0099 0.7498 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.515604 0.411759 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R03 Reach=R15 Count 5 5 Median 2.01 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:46 AM 116 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R03 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:46 AM 117 5/1/2013 10:43:47 AM 118 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R05 5 256 188.5815 84.3362 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R05) = 2.7764 95.0% LCL of Mean -6.782733 21.84515 95.0% UCL of Mean 44.76674 490.1548 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -237.008 134.1527 Unequal 4.10 -237.008 189.7205 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7507 Standard Error 84.8456 84.8456 95.0% LCL Difference -432.6623 -470.3956 95.0% UCL Difference -41.35368 -3.620423 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.7934 0.023435 Yes Difference < 0 -2.7934 0.011717 Yes Difference > 0 -2.7934 0.988283 No Difference: (Reach=R04)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.687818 0.816422 0.000012 Power (Alpha=.010) 0.365554 0.498925 0.000001 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -2.7934 0.047828 Yes Difference < 0 -2.7934 0.023914 Yes Difference > 0 -2.7934 0.976086 No Difference: (Reach=R04)-(Reach=R05) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.567642 0.744895 0.000022 Power (Alpha=.010) 0.215367 0.343890 0.000003 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 82.5312 4.5558 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000853 0.065339 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R05 Count 5 5 Median 7.85 283 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:47 AM 119 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R05 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.003968 Yes 0.996032 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6112 0.009023 Yes -2.6112 0.004512 Yes -2.6112 0.995488 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5067 0.012186 Yes -2.5067 0.006093 Yes -2.7156 0.996692 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:47 AM 120 5/1/2013 10:43:47 AM 121 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R06 5 3.704 0.9185206 0.4107749 Note: T-alpha (R e ac h =R04) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean -6.782733 2.563506 95.0% UCL of Mean 44.76674 4.844494 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 15.288 14.69264 Unequal 4.02 15.288 20.77853 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7722 Standard1 Error 9.292441 9.292441 95.0% LCL Difference -6.140409 -10.47231 95.0% UCL Difference 36.71641 41.04831 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.6452 0.138546 No Difference < 0 1.6452 0.930727 No Difference > 0 1.6452 0.069273 No Difference: (Reach=R04)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.305583 0.000777 0.444387 Power (Alpha=.010) 0.106376 0.000091 0.172109 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.6452 0.174994 No Difference < 0 1.6452 0.912503 No Difference > 0 1.6452 0.087497 No Difference: (Reach=R04)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.246541 0.001085 0.391250 Power (Alpha=.010) 0.067889 0.000165 0.121585 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 510.7430 3.0334 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000023 0.119742 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R06 Count 5 5 Median 7.85 4.15 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:47 AM 122 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R06 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 21 36 4 19 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.7810 0.074913 No 1.7810 0.962544 No 1.7810 0.037456 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.6762 0.093693 No 1.8858 0.970336 No 1.6762 0.046846 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:47 AM 123 5/1/2013 10:43:48 AM 124 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R07 5 40.32 25.25088 11.29254 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean -6.782733 8.96689 95.0% UCL of Mean 44.76674 71.67311 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -21.328 23.11396 Unequal 7.71 -21.328 32.68808 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3211 Standard Error 14.61855 14.61855 95.0% LCL Difference -55.03845 -55.2593 95.0% UCL Difference 12.38245 12.6033 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.4590 0.182691 No Difference < 0 -1.4590 0.091346 No Difference > 0 -1.4590 0.908654 No Difference: (Reach=R04)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.251235 0.378476 0.001387 Power (Alpha=.010) 0.081647 0.135581 0.000172 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.4590 0.184071 No Difference < 0 -1.4590 0.092036 No Difference > 0 -1.4590 0.907964 No Difference: (Reach=R04)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.249282 0.376732 0.001404 Power (Alpha=.010) 0.080262 0.133836 0.000176 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.4797 0.1510 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.713445 0.707762 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R07 Count 5 5 Median 7.85 29.2 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:48 AM 125 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 5 20 20 35 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.075397 No 0.924603 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.5667 0.117185 No -1.5667 0.058593 No -1.5667 0.941407 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.4623 0.143672 No -1.4623 0.071836 No -1.6711 0.952654 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:48 AM 126 5/1/2013 10:43:48 AM 127 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R08 5 8.384 8.303127 3.713272 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean -6.782733 -1.925695 95.0% UCL of Mean 44.76674 18.6937 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 10.608 15.80895 Unequal 5.25 10.608 22.35723 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5345 Standard Error 9.998456 9.998456 95.0% LCL Difference -12.44848 -14.73279 95.0% UCL Difference 33.66448 35.94879 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.0610 0.319683 No Difference < 0 1.0610 0.840158 No Difference > 0 1.0610 0.159842 No Difference: (Reach=R04)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.155262 0.004355 0.250594 Power (Alpha=.010) 0.043593 0.000608 0.076056 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.0610 0.335073 No Difference < 0 1.0610 0.832464 No Difference > 0 1.0610 0.167536 No Difference: (Reach=R04)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.141955 0.004859 0.236526 Power (Alpha=.010) 0.036404 0.000750 0.065854 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 6.2502 1.2169 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.103647 0.302053 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R08 Count 5 5 Median 7.85 4.89 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:48 AM 128 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 16 31 9 24 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.726191 No 0.273810 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.7311 0.464702 No 0.7311 0.767649 No 0.7311 0.232351 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.6267 0.530869 No 0.8356 0.798302 No 0.6267 0.265435 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.000000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:48 AM 129 5/1/2013 10:43:48 AM 130 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R09 5 22.48 13.41611 5.999866 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean -6.782733 5.8217 95.0% UCL of Mean 44.76674 39.1383 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -3.488 17.47707 Unequal 6.85 -3.488 24.7163 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3755 Standard Error 11.05347 11.05347 95.0% LCL Difference -28.97734 -29.74557 95.0% UCL Difference 22.00134 22.76957 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.3156 0.760414 No Difference < 0 -0.3156 0.380207 No Difference > 0 -0.3156 0.619793 No Difference: (Reach=R04)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.059004 0.087701 0.026483 Power (Alpha=.010) 0.012526 0.019754 0.004728 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.3156 0.761741 No Difference < 0 -0.3156 0.380871 No Difference > 0 -0.3156 0.619129 No Difference: (Reach=R04)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.058643 0.087046 0.026725 Power (Alpha=.010) 0.012364 0.019420 0.004820 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.3940 0.4674 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.418553 0.513513 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R09 Count 5 5 Median 7.85 18.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:48 AM 131 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 9 24 16 31 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.547619 No 0.273810 No 0.726191 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.7311 0.464702 No -0.7311 0.232351 No -0.7311 0.767649 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.6267 0.530869 No -0.6267 0.265435 No -0.8356 0.798302 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:48 AM 132 5/1/2013 10:43:49 AM 133 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R10 5 12.216 3.566887 1.59516 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean -6.782733 7.787125 95.0% UCL of Mean 44.76674 16.64487 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 6.776 14.89339 Unequal 4.24 6.776 21.06244 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7165 Standard Error 9.419409 9.419409 95.0% LCL Difference -14.9452 -18.81175 95.0% UCL Difference 28.4972 32.36375 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7194 0.492392 No Difference < 0 0.7194 0.753804 No Difference > 0 0.7194 0.246196 No Difference: (Reach=R 04)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.097592 0.010512 0.162317 Power (Alpha=.010) 0.024106 0.001636 0.042858 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.7194 0.509611 No Difference < 0 0.7194 0.745194 No Difference > 0 0.7194 0.254806 No Difference: (Reach=R 04)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.088167 0.011770 0.150492 Power (Alpha=.010) 0.019825 0.002033 0.036055 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 33.8689 2.1512 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.004840 0.180633 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R10 Count 5 5 Median 7.85 13.4 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:49 AM 134 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 10 25 15 30 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.690476 No 0.345238 No 0.654762 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.5222 0.601508 No -0.5222 0.300754 No -0.5222 0.699246 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.4178 0.676103 No -0.4178 0.338052 No -0.6267 0.734565 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:49 AM 135 5/1/2013 10:43:49 AM 136 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean -6.782733 8.668879 95.0% UCL of Mean 44.76674 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 3.674 15.15882 Unequal 4.53 3.674 21.43781 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6529 Standard Error 9.587281 9.587281 95.0% LCL Difference -18.43431 -21.76011 95.0% UCL Difference 25.78231 29.10811 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.3832 0.711542 No Difference < 0 0.3832 0.644229 No Difference > 0 0.3832 0.355771 No Difference: (Reach=R04)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.063308 0.022884 0.098009 Power (Alpha=.010) 0.013757 0.003990 0.022660 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.3832 0.718875 No Difference < 0 0.3832 0.640562 No Difference > 0 0.3832 0.359438 No Difference: (Reach=R04)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.061061 0.024026 0.094238 Power (Alpha=.010) 0.012806 0.004399 0.020790 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 15.0266 1.6931 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.022388 0.229419 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R11 Count 5 5 Median 7.85 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:49 AM 137 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 10 25 15 30 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.690476 No 0.345238 No 0.654762 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.5222 0.601508 No -0.5222 0.300754 No -0.5222 0.699246 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.4178 0.676103 No -0.4178 0.338052 No -0.6267 0.734565 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:49 AM 138 5/1/2013 10:43:49 AM 139 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean -6.782733 4.463322 95.0% UCL of Mean 44.76674 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 10.278 14.87654 Unequal 4.22 10.278 21.03861 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7209 Standard Error 9.408752 9.408752 95.0% LCL Difference -11.41862 -15.32225 95.0% UCL Difference 31.97462 35.87825 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.0924 0.306464 No Difference < 0 1.0924 0.846768 No Difference > 0 1.0924 0.153232 No Difference: (Reach=R04)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.161739 0.003998 0.259814 Power (Alpha=.010) 0.045940 0.000553 0.079885 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.0924 0.333098 No Difference < 0 1.0924 0.833451 No Difference > 0 1.0924 0.166549 No Difference: (Reach=R04)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.138674 0.004814 0.235118 Power (Alpha=.010) 0.033992 0.000781 0.062627 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 36.7682 2.1873 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.004132 0.177410 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R12 Count 5 5 Median 7.85 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:49 AM 140 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 12 27 13 28 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 1.000000 No 0.500000 No 0.500000 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.1044 0.916815 No -0.1044 0.458407 No -0.1044 0.541593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.0000 1.000000 No 0.0000 0.500000 No -0.2089 0.582734 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:49 AM 141 5/1/2013 10:43:49 AM 142 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (R e ac h =R04) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean -6.782733 7.741103 95.0% UCL of Mean 44.76674 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 9.22 14.72377 Unequal 4.05 9.22 20.82256 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7631 Standard1 Error 9.312131 9.312131 95.0% LCL Difference -12.25381 -16.51005 95.0% UCL Difference 30.69381 34.95005 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.9901 0.351120 No Difference < 0 0.9901 0.824440 No Difference > 0 0.9901 0.175560 No Difference: (Reach=R 04)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.141378 0.005269 0.230452 Power (Alpha=.010) 0.038668 0.000752 0.067931 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.9901 0.377530 No Difference < 0 0.9901 0.811235 No Difference > 0 0.9901 0.188765 No Difference: (Reach=R 04)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.121250 0.006297 0.207974 Power (Alpha=.010) 0.028751 0.001049 0.053228 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 161.0692 2.7789 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000227 0.134074 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R13 Count 5 5 Median 7.85 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:50 AM 143 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 11 26 14 29 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.841270 No 0.420635 No 0.579365 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.3133 0.754023 No -0.3133 0.377011 No -0.3133 0.622989 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.2089 0.834532 No -0.2089 0.417266 No -0.4178 0.661948 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:50 AM 144 5/1/2013 10:43:50 AM 145 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean -6.782733 4.72547 95.0% UCL of Mean 44.76674 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 9.876 14.88971 Unequal 4.23 9.876 21.05723 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7175 Standard Error 9.41708 9.41708 95.0% LCL Difference -11.83983 -15.71445 95.0% UCL Difference 31.59183 35.46645 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.0487 0.324948 No Difference < 0 1.0487 0.837526 No Difference > 0 1.0487 0.162474 No Difference: (Reach=R04)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.152794 0.004502 0.247053 Power (Alpha=.010) 0.042706 0.000631 0.074604 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.0487 0.350461 No Difference < 0 1.0487 0.824770 No Difference > 0 1.0487 0.175230 No Difference: (Reach=R04)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.131785 0.005368 0.224216 Power (Alpha=.010) 0.032000 0.000876 0.059006 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 34.4631 2.1984 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.004681 0.176437 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R14 Count 5 5 Median 7.85 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:50 AM 146 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 12 27 13 28 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 1.000000 No 0.500000 No 0.500000 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.1044 0.916815 No -0.1044 0.458407 No -0.1044 0.541593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.0000 1.000000 No 0.0000 0.500000 No -0.2089 0.582734 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:50 AM 147 5/1/2013 10:43:50 AM 148 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R04 5 18.992 20.75822 9.283358 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R04) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean -6.782733 5.414244 95.0% UCL of Mean 44.76674 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 11.046 14.74892 Unequal 4.08 11.046 20.85812 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7558 Standard Error 9.328035 9.328035 95.0% LCL Difference -10.46449 -14.66057 95.0% UCL Difference 32.55649 36.75257 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1842 0.270332 No Difference < 0 1.1842 0.864834 No Difference > 0 1.1842 0.135166 No Difference: (Reach=R 04)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.181771 0.003098 0.287701 Power (Alpha=.010) 0.053402 0.000417 0.091890 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1842 0.300752 No Difference < 0 1.1842 0.849624 No Difference > 0 1.1842 0.150376 No Difference: (Reach=R 04)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.152644 0.003853 0.257352 Power (Alpha=.010) 0.037887 0.000622 0.069787 Tests of Assumptions Section Assumption Skewness Normality (Reach=R04) Kurtosis Normality (Reach=R04) Omnibus Normality (Reach=R04) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 103.6442 2.6313 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000544 0.143434 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R04 Reach=R15 Count 5 5 Median 7.85 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:50 AM 149 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R04 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 13 28 12 27 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 1.000000 No 0.579365 No 0.420635 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.1044 0.916815 No 0.1044 0.541593 No 0.1044 0.458407 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.0000 1.000000 No 0.2089 0.582734 No 0.0000 0.500000 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:50 AM 150 5/1/2013 10:43:50 AM 151 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R06 5 3.704 0.9185206 0.4107749 Note: T-alpha (Reach:=R05) = 2.7764, T-alpha (Reach=R06) = 2.7764 95.0% LCL of Mean 21.84515 2.563506 95.0% UCL of Mean 490.1548 4.844494 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 252.296 133.3488 Unequal 4.00 252.296 188.5837 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7764 Standard Error 84.33721 84.33721 95.0% LCL Difference 57.81405 18.14275 95.0% UCL Difference 446.778 486.4492 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9915 0.017294 Yes Difference < 0 2.9915 0.991353 No Difference > 0 2.9915 0.008647 Yes Difference: (Reach=R05)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.745673 0.000005 0.860116 Power (Alpha=.010) 0.424689 0.000000 0.562941 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9915 0.040275 Yes Difference < 0 2.9915 0.979862 No Difference > 0 2.9915 0.020138 Yes Difference: (Reach=R05)-(Reach=R06) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.616959 0.000010 0.789165 Power (Alpha=.010) 0.244064 0.000001 0.384085 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 Probability Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 42152.2141 0.000000 5.8288 0.042224 Reject equal variances Reject equal variances Median Statistics Variable Reach=R05 Reach=R06 Count 5 5 Median 283 4.15 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:50 AM 152 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R06 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6191 0.008816 Yes 2.6191 0.995592 No 2.6191 0.004408 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5143 0.011925 Yes 2.7239 0.996774 No 2.5143 0.005963 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:51 AM 153 5/1/2013 10:43:51 AM 154 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R07 5 40.32 25.25088 11.29254 Note: T-alpha (Reach:=R05) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 21.84515 8.96689 95.0% UCL of Mean 490.1548 71.67311 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 215.68 134.5373 Unequal 4.14 215.68 190.2645 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7390 Standard Error 85.08888 85.08888 95.0% LCL Difference 19.46469 -17.37503 95.0% UCL Difference 411.8953 448.735 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.5348 0.034995 Yes Difference < 0 2.5348 0.982502 No Difference > 0 2.5348 0.017498 Yes Difference: (Reach=R05)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.604735 0.000033 0.747863 Power (Alpha=.010) 0.292799 0.000003 0.415372 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.5348 0.062218 No Difference < 0 2.5348 0.968891 No Difference > 0 2.5348 0.031109 Yes Difference: (Reach=R05)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.495153 0.000056 0.675848 Power (Alpha=.010) 0.175528 0.000008 0.287272 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 55.7757 4.2104 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001839 0.074285 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R07 Count 5 5 Median 283 29.2 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:51 AM 155 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R07 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:51 AM 156 5/1/2013 10:43:51 AM 157 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R08 5 8.384 8.303127 3.713272 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 21.84515 -1.925695 95.0% UCL of Mean 490.1548 18.6937 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 247.616 133.4764 Unequal 4.02 247.616 188.7642 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7722 Standard Error 84.41792 84.41792 95.0% LCL Difference 52.94794 13.59086 95.0% UCL Difference 442.2841 481.6411 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9332 0.018905 Yes Difference < 0 2.9332 0.990548 No Difference > 0 2.9332 0.009452 Yes Difference: (Reach=R05)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.729258 0.000006 0.848067 Power (Alpha=.010) 0.407068 0.000001 0.544217 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9332 0.042474 Yes Difference < 0 2.9332 0.978763 No Difference > 0 2.9332 0.021237 Yes Difference: (Reach=R05)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.601925 0.000013 0.776210 Power (Alpha=.010) 0.234731 0.000002 0.371319 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 515.8396 5.4226 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000022 0.048263 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05 Reach=R08 Count 5 5 Median 283 4.89 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:51 AM 158 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:51 AM 159 5/1/2013 10:43:51 AM 160 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R09 5 22.48 13.41611 5.999866 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 21.84515 5.8217 95.0% UCL of Mean 490.1548 39.1383 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 233.52 133.6843 Unequal 4.04 233.52 189.0581 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7655 Standard Error 84.54936 84.54936 95.0% LCL Difference 38.54882 -0.3018479 95.0% UCL Difference 428.4912 467.3419 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7619 0.024601 Yes Difference < 0 2.7619 0.987699 No Difference > 0 2.7619 0.012301 Yes Difference: (Reach=R05)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.678119 0.000013 0.808767 Power (Alpha=.010) 0.356391 0.000001 0.488700 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7619 0.050185 No Difference < 0 2.7619 0.974908 No Difference > 0 2.7619 0.025092 Yes Difference: (Reach=R05)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.555234 0.000025 0.734517 Power (Alpha=.010) 0.206901 0.000003 0.332786 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 197.5809 5.1351 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000152 0.053215 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R05 Reach=R09 Count 5 5 Median 283 18.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:51 AM 161 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:51 AM 162 5/1/2013 10:43:52 AM 163 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R10 5 12.216 3.566887 1.59516 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R10) = 2.7764 95.0% LCL of Mean 21.84515 7.787125 95.0% UCL of Mean 490.1548 16.64487 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 243.784 133.3711 Unequal 4.00 243.784 188.6152 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7757 Standard Error 84.3513 84.3513 95.0% LCL Difference 49.26957 9.653291 95.0% UCL Difference 438.2984 477.9147 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.8901 0.020196 Yes Difference < 0 2.8901 0.989902 No Difference > 0 2.8901 0.010098 Yes Difference: (Reach=R 05)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.716782 0.000008 0.838724 Power (Alpha=.010) 0.394143 0.000001 0.530294 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.8901 0.044520 Yes Difference < 0 2.8901 0.977740 No Difference > 0 2.8901 0.022260 Yes Difference: (Reach=R 05)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.589114 0.000015 0.765347 Power (Alpha=.010) 0.226405 0.000002 0.360157 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2795.2428 5.6401 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000001 0.044903 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05 Reach=R10 Count 5 5 Median 283 13.4 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:52 AM 164 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:52 AM 165 5/1/2013 10:43:52 AM 166 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 21.84515 8.668879 95.0% UCL of Mean 490.1548 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 240.682 133.401 Unequal 4.01 240.682 188.6575 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7747 Standard Error 84.3702 84.3702 95.0% LCL Difference 46.12396 6.581433 95.0% UCL Difference 435.2401 474.7826 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.8527 0.021390 Yes Difference < 0 2.8527 0.989305 No Difference > 0 2.8527 0.010695 Yes Difference: (Reach=R05)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.705732 0.000009 0.830315 Power (Alpha=.010) 0.383010 0.000001 0.518174 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.8527 0.046182 Yes Difference < 0 2.8527 0.976909 No Difference > 0 2.8527 0.023091 Yes Difference: (Reach=R05)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.578857 0.000018 0.756287 Power (Alpha=.010) 0.220205 0.000002 0.351612 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1240.1601 5.5276 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000004 0.046602 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05 Reach=R11 Count 5 5 Median 283 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:52 AM 167 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:52 AM 168 5/1/2013 10:43:52 AM 169 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 21.84515 4.463322 95.0% UCL of Mean 490.1548 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 247.286 133.3692 Unequal 4.00 247.286 188.6126 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7757 Standard1 Error 84.35011 84.35011 95.0% LCL Difference 52.77431 13.15339 95.0% UCL Difference 441.7977 481.4186 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9317 0.018950 Yes Difference < 0 2.9317 0.990525 No Difference > 0 2.9317 0.009475 Yes Difference: (Reach=R05)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.728813 0.000006 0.847737 Power (Alpha=.010) 0.406600 0.000001 0.543716 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9317 0.042708 Yes Difference < 0 2.9317 0.978646 No Difference > 0 2.9317 0.021354 Yes Difference: (Reach=R05)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.600658 0.000013 0.775325 Power (Alpha=.010) 0.233624 0.000002 0.369984 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3034.5186 5.6527 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000001 0.044717 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05 Reach=R12 Count 5 5 Median 283 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:52 AM 170 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:52 AM 171 5/1/2013 10:43:53 AM 172 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (Reach:=R05) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 21.84515 7.741103 95.0% UCL of Mean 490.1548 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 246.228 133.3523 Unequal 4.00 246.228 188.5886 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7763 Standard Error 84.33938 84.33938 95.0% LCL Difference 51.74104 12.07823 95.0% UCL Difference 440.715 480.3778 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9195 0.019306 Yes Difference < 0 2.9195 0.990347 No Difference > 0 2.9195 0.009653 Yes Difference: (Reach=R 05)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.725316 0.000007 0.845133 Power (Alpha=.010) 0.402942 0.000001 0.539790 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9195 0.043258 Yes Difference < 0 2.9195 0.978371 No Difference > 0 2.9195 0.021629 Yes Difference: (Reach=R 05)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.597150 0.000013 0.772344 Power (Alpha=.010) 0.231364 0.000002 0.366942 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 Probability Decision(.050) 1.000000 Cannot reject normality 0.0000 1.000000 Cannot reject normality 13293.2302 0.000000 5.7767 0.042944 Reject equal variances Reject equal variances Median Statistics Variable Reach=R05 Reach=R13 Count 5 5 Median 283 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:53 AM 173 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:53 AM 174 5/1/2013 10:43:53 AM 175 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 21.84515 4.72547 95.0% UCL of Mean 490.1548 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 246.884 133.3707 Unequal 4.00 246.884 188.6146 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7757 Standard Error 84.35104 84.35104 95.0% LCL Difference 52.37017 12.75288 95.0% UCL Difference 441.3978 481.0151 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9269 0.019090 Yes Difference < 0 2.9269 0.990455 No Difference > 0 2.9269 0.009545 Yes Difference: (Reach=R05)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.727437 0.000007 0.846714 Power (Alpha=.010) 0.405157 0.000001 0.542169 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9269 0.042911 Yes Difference < 0 2.9269 0.978544 No Difference > 0 2.9269 0.021456 Yes Difference: (Reach=R05)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.599340 0.000013 0.774192 Power (Alpha=.010) 0.232795 0.000002 0.368857 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2844.2807 5.6520 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000001 0.044728 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05 Reach=R14 Count 5 5 Median 283 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:53 AM 176 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:53 AM 177 5/1/2013 10:43:53 AM 178 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R05 5 256 188.5815 84.3362 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (R e ac h =R05) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 21.84515 5.414244 95.0% UCL of Mean 490.1548 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 248.054 133.355 Unequal 4.00 248.054 188.5925 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7762 Standard1 Error 84.34114 84.34114 95.0% LCL Difference 53.56299 13.90705 95.0% UCL Difference 442.545 482.201 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9411 0.018679 Yes Difference < 0 2.9411 0.990661 No Difference > 0 2.9411 0.009339 Yes Difference: (Reach=R 05)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.731503 0.000006 0.849732 Power (Alpha=.010) 0.409436 0.000001 0.546750 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.9411 0.042331 Yes Difference < 0 2.9411 0.978834 No Difference > 0 2.9411 0.021166 Yes Difference: (Reach=R 05)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.603152 0.000012 0.777485 Power (Alpha=.010) 0.235167 0.000002 0.372096 Tests of Assumptions Section Assumption Skewness Normality (Reach=R05) Kurtosis Normality (Reach=R05) Omnibus Normality (Reach=R05) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 8553.8721 5.7454 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000000 0.043382 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R05 Reach=R15 Count 5 5 Median 283 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:53 AM 179 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R05 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:53 AM 180 5/1/2013 10:43:54 AM 181 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 3.704 0.9185206 0.4107749 Reach=R07 5 40.32 25.25088 11.29254 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R07) = 2.7764 95.0% LCL of Mean 2.563506 8.96689 95.0% UCL of Mean 4.844494 71.67311 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -36.616 17.86688 Unequal 4.01 -36.616 25.26758 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7736 Standard Error 11.30001 11.30001 95.0% LCL Difference -62.67386 -67.95721 95.0% UCL Difference -10.55814 -5.274785 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.2404 0.011871 Yes Difference < 0 -3.2404 0.005936 Yes Difference > 0 -3.2404 0.994064 No Difference: (Reach=R06)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.809378 0.904125 0.000002 Power (Alpha=.010) 0.500955 0.640636 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.2404 0.031543 Yes Difference < 0 -3.2404 0.015771 Yes Difference > 0 -3.2404 0.984229 No Difference: (Reach=R06)-(Reach=R07) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.683264 0.841671 0.000004 Power (Alpha=.010) 0.291193 0.445449 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 755.7451 4.9940 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000010 0.055884 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R07 Count 5 5 Median 4.15 29.2 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:54 AM 182 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R07 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6191 0.008816 Yes -2.6191 0.004408 Yes -2.6191 0.995592 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5143 0.011925 Yes -2.5143 0.005963 Yes -2.7239 0.996774 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:54 AM 183 5/1/2013 10:43:54 AM 184 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 3.704 0.9185206 0.4107749 Reach=R08 5 8.384 8.303127 3.713272 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 2.563506 -1.925695 95.0% UCL of Mean 4.844494 18.6937 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -4.68 5.907013 Unequal 4.10 -4.68 8.353778 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7505 Standard Error 3.735923 3.735923 95.0% LCL Difference -13.29505 -14.9556 95.0% UCL Difference 3.935054 5.595597 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.2527 0.245691 No Difference < 0 -1.2527 0.122845 No Difference > 0 -1.2527 0.877155 No Difference: (Reach=R06)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.197792 0.309393 0.002550 Power (Alpha=.010) 0.059589 0.101677 0.000336 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.2527 0.277040 No Difference < 0 -1.2527 0.138520 No Difference > 0 -1.2527 0.861480 No Difference: (Reach=R06)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.165270 0.276289 0.003213 Power (Alpha=.010) 0.041737 0.076584 0.000513 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 81.7157 1.8572 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000870 0.210068 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R08 Count 5 5 Median 4.15 4.89 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:54 AM 185 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R08 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 8 23 17 32 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.9429 0.345742 No -0.9429 0.172871 No -0.9429 0.827129 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.8381 0.401965 No -0.8381 0.200983 No -1.0476 0.852599 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:54 AM 186 5/1/2013 10:43:54 AM 187 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 3.704 0.9185206 0.4107749 Reach=R09 5 22.48 13.41611 5.999866 Note: T-alpha (R e ac h =R06) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 2.563506 5.8217 95.0% UCL of Mean 4.844494 39.1383 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -18.776 9.508829 Unequal 4.04 -18.776 13.44752 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7663 Standard Error 6.013912 6.013912 95.0% LCL Difference -32.6441 -35.41232 95.0% UCL Difference -4.907894 -2.139684 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.1221 0.014184 Yes Difference < 0 -3.1221 0.007092 Yes Difference > 0 -3.1221 0.992908 No Difference: (Reach=R06)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.780430 0.884683 0.000003 Power (Alpha=.010) 0.464586 0.604256 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.1221 0.035007 Yes Difference < 0 -3.1221 0.017504 Yes Difference > 0 -3.1221 0.982496 No Difference: (Reach=R06)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.654505 0.819119 0.000006 Power (Alpha=.010) 0.270724 0.418746 0.000001 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 213.3416 2.4888 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000130 0.153310 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R09 Count 5 5 Median 4.15 18.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:54 AM 188 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R09 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6191 0.008816 Yes -2.6191 0.004408 Yes -2.6191 0.995592 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5143 0.011925 Yes -2.5143 0.005963 Yes -2.7239 0.996774 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:54 AM 189 5/1/2013 10:43:54 AM 190 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard1 Variable Count Mean Deviation Error Reach=R06 5 3.704 0.9185206 0.4107749 Reach=R10 5 12.216 3.566887 1.59516 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 2.563506 7.787125 95.0% UCL of Mean 4.844494 16.64487 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -8.512 2.604454 Unequal 4.53 -8.512 3.683254 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6533 Standard1 Error 1.647201 1.647201 95.0% LCL Difference -12.31045 -12.88247 95.0% UCL Difference -4.713547 -4.141529 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.1676 0.000856 Yes Difference < 0 -5.1676 0.000428 Yes Difference > 0 -5.1676 0.999572 No Difference: (Reach=R 06)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.994172 0.998794 0.000000 Power (Alpha=.010) 0.928391 0.971262 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -5.1676 0.004727 Yes Difference < 0 -5.1676 0.002363 Yes Difference > 0 -5.1676 0.997637 No Difference: (Reach=R 06)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.975562 0.995566 0.000000 Power (Alpha=.010) 0.753636 0.885575 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 15.0800 3.9695 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.022243 0.081473 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R10 Count 5 5 Median 4.15 13.4 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:54 AM 191 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R10 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6191 0.008816 Yes -2.6191 0.004408 Yes -2.6191 0.995592 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5143 0.011925 Yes -2.5143 0.005963 Yes -2.7239 0.996774 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:55 AM 192 5/1/2013 10:43:55 AM 193 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 3.704 0.9185206 0.4107749 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 2.563506 8.668879 95.0% UCL of Mean 4.844494 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -11.614 3.841861 Unequal 4.24 -11.614 5.433213 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7167 Standard Error 2.429806 2.429806 95.0% LCL Difference -17.21714 -18.21503 95.0% UCL Difference -6.010856 -5.012971 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.7798 0.001391 Yes Difference < 0 -4.7798 0.000696 Yes Difference > 0 -4.7798 0.999304 No Difference: (Reach=R06)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.985854 0.996404 0.000000 Power (Alpha=.010) 0.879717 0.944420 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.7798 0.007610 Yes Difference < 0 -4.7798 0.003805 Yes Difference > 0 -4.7798 0.996195 No Difference: (Reach=R06)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.946732 0.987723 0.000000 Power (Alpha=.010) 0.643262 0.807283 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 33.9893 6.2509 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.004808 0.036931 Cannot reject normality Reject equal variances Reject equal variances Median Statistics Variable Reach=R06 Reach=R11 Count 5 5 Median 4.15 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:55 AM 194 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R11 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6191 0.008816 Yes -2.6191 0.004408 Yes -2.6191 0.995592 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5143 0.011925 Yes -2.5143 0.005963 Yes -2.7239 0.996774 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:55 AM 195 5/1/2013 10:43:55 AM 196 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 3.704 0.9185206 0.4107749 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 2.563506 4.463322 95.0% UCL of Mean 4.844494 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -5.01 2.506308 Unequal 4.57 -5.01 3.544455 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6445 Standard Error 1.585128 1.585128 95.0% LCL Difference -8.665313 -9.201859 95.0% UCL Difference -1.354687 -0.8181409 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.1606 0.013382 Yes Difference < 0 -3.1606 0.006691 Yes Difference > 0 -3.1606 0.993309 No Difference: (Reach=R06)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.790133 0.891305 0.000002 Power (Alpha=.010) 0.476427 0.616234 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.1606 0.028371 Yes Difference < 0 -3.1606 0.014185 Yes Difference > 0 -3.1606 0.985815 No Difference: (Reach=R06)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.695880 0.844054 0.000004 Power (Alpha=.010) 0.316944 0.469567 0.000001 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 13.8909 2.1672 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.025847 0.179196 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R12 Count 5 5 Median 4.15 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:55 AM 197 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R12 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6191 0.008816 Yes -2.6191 0.004408 Yes -2.6191 0.995592 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5143 0.011925 Yes -2.5143 0.005963 Yes -2.7239 0.996774 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:55 AM 198 5/1/2013 10:43:55 AM 199 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 3.704 0.9185206 0.4107749 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (R e ac h =R06) = 2.7764, T-alpha (Reach=R13) = 2.7764 95.0% LCL of Mean 2.563506 7.741103 95.0% UCL of Mean 4.844494 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.068 1.326452 Unequal 6.29 -6.068 1.875886 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4194 Standard Error 0.8389219 0.8389219 95.0% LCL Difference -8.002558 -8.097696 95.0% UCL Difference -4.133442 -4.038303 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.2331 0.000090 Yes Difference < 0 -7.2331 0.000045 Yes Difference > 0 -7.2331 0.999955 No Difference: (Reach=R 06)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999990 0.999999 0.000000 Power (Alpha=.010) 0.998577 0.999777 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -7.2331 0.000284 Yes Difference < 0 -7.2331 0.000142 Yes Difference > 0 -7.2331 0.999858 No Difference: (Reach=R 06)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.999963 0.999998 0.000000 Power (Alpha=.010) 0.994509 0.999108 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.1710 0.9495 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.289764 0.358397 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R13 Count 5 5 Median 4.15 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:55 AM 200 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R13 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6191 0.008816 Yes -2.6191 0.004408 Yes -2.6191 0.995592 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5143 0.011925 Yes -2.5143 0.005963 Yes -2.7239 0.996774 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:56 AM 201 5/1/2013 10:43:56 AM 202 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R06 5 3.704 0.9185206 0.4107749 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 2.563506 4.72547 95.0% UCL of Mean 4.844494 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -5.412 2.583313 Unequal 4.54 -5.412 3.653356 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6515 Standard Error 1.63383 1.63383 95.0% LCL Difference -9.17962 -9.744023 95.0% UCL Difference -1.64438 -1.079977 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3125 0.010659 Yes Difference < 0 -3.3125 0.005330 Yes Difference > 0 -3.3125 0.994670 No Difference: (Reach=R06)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.825807 0.914725 0.000001 Power (Alpha=.010) 0.523109 0.662204 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -3.3125 0.024497 Yes Difference < 0 -3.3125 0.012248 Yes Difference > 0 -3.3125 0.987752 No Difference: (Reach=R06)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.732551 0.870944 0.000002 Power (Alpha=.010) 0.347905 0.507134 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 14.8200 3.1991 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.022964 0.111475 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R14 Count 5 5 Median 4.15 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:56 AM 203 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R14 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6191 0.008816 Yes -2.6191 0.004408 Yes -2.6191 0.995592 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5143 0.011925 Yes -2.5143 0.005963 Yes -2.7239 0.996774 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:56 AM 204 5/1/2013 10:43:56 AM 205 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard1 Variable Count Mean Deviation Error Reach=R06 5 3.704 0.9185206 0.4107749 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R06) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 2.563506 5.414244 95.0% UCL of Mean 4.844494 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -4.242 1.58133 Unequal 5.56 -4.242 2.236339 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4947 Standard1 Error 1.000121 1.000121 95.0% LCL Difference -6.548283 -6.737021 95.0% UCL Difference -1.935717 -1.746979 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.2415 0.002832 Yes Difference < 0 -4.2415 0.001416 Yes Difference > 0 -4.2415 0.998584 No Difference: (Reach=R 06)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.958583 0.986300 0.000000 Power (Alpha=.010) 0.778610 0.877941 0.000000 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -4.2415 0.006445 Yes Difference < 0 -4.2415 0.003222 Yes Difference > 0 -4.2415 0.996778 No Difference: (Reach=R 06)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.931253 0.978320 0.000000 Power (Alpha=.010) 0.656801 0.800723 0.000000 Tests of Assumptions Section Assumption Skewness Normality (Reach=R06) Kurtosis Normality (Reach=R06) Omnibus Normality (Reach=R06) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.9279 2.0009 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.151545 0.194921 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R06 Reach=R15 Count 5 5 Median 4.15 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:56 AM 206 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R06 Reach=R15 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 0 15 25 40 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -2.6191 0.008816 Yes -2.6191 0.004408 Yes -2.6191 0.995592 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -2.5143 0.011925 Yes -2.5143 0.005963 Yes -2.7239 0.996774 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 1.000000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:56 AM 207 5/1/2013 10:43:56 AM 208 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 40.32 25.25088 11.29254 Reach=R08 5 8.384 8.303127 3.713272 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R08) = 2.7764 95.0% LCL of Mean 8.96689 -1.925695 95.0% UCL of Mean 71.67311 18.6937 Confidence-Limits of Difference Section Variance Assumption DF Equal 8 Unequal 4.86 Note: T-alpha (Equal) = 2.3060, Mean Standard Difference Deviation 31.936 18.7956 31.936 26.58099 T-alpha (Unequal) = 2.5938 Standard Error 11.88738 11.88738 95.0% LCL Difference 4.523654 1.101993 95.0% UCL Difference 59.34835 62.77001 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.6865 0.027644 Yes Difference < 0 2.6865 0.986178 No Difference > 0 2.6865 0.013822 Yes Difference: (Reach=R07)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.654380 0.000018 0.789638 Power (Alpha=.010) 0.334758 0.000002 0.464226 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.6865 0.044809 Yes Difference < 0 2.6865 0.977595 No Difference > 0 2.6865 0.022405 Yes Difference: (Reach=R07)-(Reach=R08) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.574287 0.000027 0.741199 Power (Alpha=.010) 0.233599 0.000003 0.360781 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 9.2485 2.4995 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.053410 0.152539 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R08 Count 5 5 Median 29.2 4.89 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:56 AM 209 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R08 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 39 1 16 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.992063 No 0.007937 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.4023 0.016294 Yes 2.4023 0.991853 No 2.4023 0.008147 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.2978 0.021572 Yes 2.5067 0.993907 No 2.2978 0.010786 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:56 AM 210 5/1/2013 10:43:57 AM 211 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 40.32 25.25088 11.29254 Reach=R09 5 22.48 13.41611 5.999866 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean 8.96689 5.8217 95.0% UCL of Mean 71.67311 39.1383 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 17.84 20.21879 Unequal 6.09 17.84 28.59369 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4380 Standard Error 12.78749 12.78749 95.0% LCL Difference -11.648 -13.33608 95.0% UCL Difference 47.328 49.01608 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.3951 0.200493 No Difference < 0 1.3951 0.899753 No Difference > 0 1.3951 0.100247 No Difference: (Reach=R07)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.233884 0.001681 0.356553 Power (Alpha=.010) 0.074244 0.000212 0.124358 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.3951 0.211726 No Difference < 0 1.3951 0.894137 No Difference > 0 1.3951 0.105863 No Difference: (Reach=R07)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.219396 0.001846 0.343078 Power (Alpha=.010) 0.064804 0.000254 0.112027 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.5424 1.2947 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.248111 0.288105 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R09 Count 5 5 Median 29.2 18.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:57 AM 212 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 18 33 7 22 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.845238 No 0.154762 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.1489 0.250592 No 1.1489 0.874704 No 1.1489 0.125296 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.0445 0.296270 No 1.2534 0.894962 No 1.0445 0.148135 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.200000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:57 AM 213 5/1/2013 10:43:57 AM 214 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 40.32 25.25088 11.29254 Reach=R10 5 12.216 3.566887 1.59516 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 8.96689 7.787125 95.0% UCL of Mean 71.67311 16.64487 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 28.104 18.03233 Unequal 4.16 28.104 25.50156 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7349 Standard Error 11.40464 11.40464 95.0% LCL Difference 1.804841 -3.087027 95.0% UCL Difference 54.40316 59.29503 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.4643 0.039059 Yes Difference < 0 2.4643 0.980471 No Difference > 0 2.4643 0.019529 Yes Difference: (Reach=R 07)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.581002 0.000043 0.727053 Power (Alpha=.010) 0.274138 0.000004 0.393035 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.4643 0.066965 No Difference < 0 2.4643 0.966517 No Difference > 0 2.4643 0.033483 Yes Difference: (Reach=R 07)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.475276 0.000073 0.655744 Power (Alpha=.010) 0.165486 0.000010 0.272585 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 50.1158 3.8589 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.002266 0.085076 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R10 Count 5 5 Median 29.2 13.4 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:57 AM 215 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 22 37 3 18 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.972222 No 0.027778 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.9845 0.047202 Yes 1.9845 0.976399 No 1.9845 0.023601 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.8800 0.060103 No 2.0889 0.981643 No 1.8800 0.030051 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:57 AM 216 5/1/2013 10:43:57 AM 217 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 40.32 25.25088 11.29254 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 8.96689 8.668879 95.0% UCL of Mean 71.67311 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 25.002 18.25217 Unequal 4.36 25.002 25.81246 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6885 Standard Error 11.54368 11.54368 95.0% LCL Difference -1.617781 -6.033576 95.0% UCL Difference 51.62178 56.03757 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1659 0.062219 No Difference < 0 2.1659 0.968891 No Difference > 0 2.1659 0.031109 Yes Difference: (Reach=R07)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.478139 0.000132 0.630570 Power (Alpha=.010) 0.202013 0.000014 0.302867 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1659 0.090620 No Difference < 0 2.1659 0.954690 No Difference > 0 2.1659 0.045310 Yes Difference: (Reach=R07)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.396454 0.000198 0.569604 Power (Alpha=.010) 0.129981 0.000027 0.218483 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 22.2348 3.2437 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.010795 0.109382 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R11 Count 5 5 Median 29.2 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:57 AM 218 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 21 36 4 19 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.952381 No 0.047619 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.7756 0.075800 No 1.7756 0.962100 No 1.7756 0.037900 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.6711 0.094693 No 1.8800 0.969949 No 1.6711 0.047346 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:57 AM 219 5/1/2013 10:43:58 AM 220 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 40.32 25.25088 11.29254 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 8.96689 4.463322 95.0% UCL of Mean 71.67311 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 31.606 18.01841 Unequal 4.15 31.606 25.48189 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7381 Standard Error 11.39585 11.39585 95.0% LCL Difference 5.327133 0.4034891 95.0% UCL Difference 57.88487 62.80851 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7735 0.024167 Yes Difference < 0 2.7735 0.987916 No Difference > 0 2.7735 0.012084 Yes Difference: (Reach=R07)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.681687 0.000012 0.811595 Power (Alpha=.010) 0.359740 0.000001 0.492447 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7735 0.048172 Yes Difference < 0 2.7735 0.975914 No Difference > 0 2.7735 0.024086 Yes Difference: (Reach=R07)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.564888 0.000023 0.741669 Power (Alpha=.010) 0.214734 0.000003 0.342455 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 54.4057 3.8975 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001931 0.083795 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R12 Count 5 5 Median 29.2 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:58 AM 221 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R12 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 24.5 39.5 0.5 15.5 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.5143 0.011925 Yes 2.5143 0.994037 No 2.5143 0.005963 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.4096 0.015971 Yes 2.6191 0.995592 No 2.4096 0.007985 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:58 AM 222 5/1/2013 10:43:58 AM 223 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 40.32 25.25088 11.29254 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (R e ac h =R07) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 8.96689 7.741103 95.0% UCL of Mean 71.67311 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 30.548 17.89249 Unequal 4.03 30.548 25.3038 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7674 Standard1 Error 11.3162 11.3162 95.0% LCL Difference 4.452789 -0.7679722 95.0% UCL Difference 56.64321 61.86397 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.6995 0.027095 Yes Difference < 0 2.6995 0.986452 No Difference > 0 2.6995 0.013548 Yes Difference: (Reach=R 07)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.658504 0.000017 0.793001 Power (Alpha=.010) 0.338439 0.000002 0.468423 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.6995 0.053643 No Difference < 0 2.6995 0.973179 No Difference > 0 2.6995 0.026821 Yes Difference: (Reach=R 07)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.536898 0.000031 0.717664 Power (Alpha=.010) 0.196300 0.000004 0.317984 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 238.3337 4.6731 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000104 0.062619 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R13 Count 5 5 Median 29.2 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:58 AM 224 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 39 1 16 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.992063 No 0.007937 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.4023 0.016294 Yes 2.4023 0.991853 No 2.4023 0.008147 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.2978 0.021572 Yes 2.5067 0.993907 No 2.2978 0.010786 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:58 AM 225 5/1/2013 10:43:58 AM 226 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 40.32 25.25088 11.29254 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R07) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 8.96689 4.72547 95.0% UCL of Mean 71.67311 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 31.204 18.02929 Unequal 4.16 31.204 25.49726 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7356 Standard Error 11.40272 11.40272 95.0% LCL Difference 4.909276 0.01047993 95.0% UCL Difference 57.49872 62.39752 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7365 0.025586 Yes Difference < 0 2.7365 0.987207 No Difference > 0 2.7365 0.012793 Yes Difference: (Reach=R07)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.670198 0.000015 0.802446 Power (Alpha=.010) 0.349051 0.000001 0.480449 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.7365 0.049951 Yes Difference < 0 2.7365 0.975024 No Difference > 0 2.7365 0.024976 Yes Difference: (Reach=R07)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.554804 0.000026 0.732367 Power (Alpha=.010) 0.208941 0.000004 0.334345 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 50.9950 3.9191 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.002191 0.083092 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R14 Count 5 5 Median 29.2 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:58 AM 227 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 24 39 1 16 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.015873 Yes 0.992063 No 0.007937 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.4023 0.016294 Yes 2.4023 0.991853 No 2.4023 0.008147 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.2978 0.021572 Yes 2.5067 0.993907 No 2.2978 0.010786 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:58 AM 228 5/1/2013 10:43:59 AM 229 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R07 5 40.32 25.25088 11.29254 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (R e ac h =R07) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 8.96689 5.414244 95.0% UCL of Mean 71.67311 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 32.374 17.91319 Unequal 4.05 32.374 25.33307 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7624 Standard Error 11.32929 11.32929 95.0% LCL Difference 6.248601 1.077884 95.0% UCL Difference 58.4994 63.67012 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.8575 0.021231 Yes Difference < 0 2.8575 0.989384 No Difference > 0 2.8575 0.010616 Yes Difference: (Reach=R 07)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.707178 0.000009 0.831423 Power (Alpha=.010) 0.384451 0.000001 0.519750 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.8575 0.045347 Yes Difference < 0 2.8575 0.977327 No Difference > 0 2.8575 0.022673 Yes Difference: (Reach=R 07)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.583109 0.000017 0.759324 Power (Alpha=.010) 0.223801 0.000002 0.355996 Tests of Assumptions Section Assumption Skewness Normality (Reach=R07) Kurtosis Normality (Reach=R07) Omnibus Normality (Reach=R07) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 153.3620 4.4853 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.000251 0.067049 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R07 Reach=R15 Count 5 5 Median 29.2 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:59 AM 230 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R07 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:59 AM 231 5/1/2013 10:43:59 AM 232 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 8.384 8.303127 3.713272 Reach=R09 5 22.48 13.41611 5.999866 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R09) = 2.7764 95.0% LCL of Mean -1.925695 5.8217 95.0% UCL of Mean 18.6937 39.1383 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -14.096 11.15648 Unequal 6.67 -14.096 15.77764 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3884 Standard Error 7.055975 7.055975 95.0% LCL Difference -30.36711 -30.94835 95.0% UCL Difference 2.175108 2.756355 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.9977 0.080799 No Difference < 0 -1.9977 0.040399 Yes Difference > 0 -1.9977 0.959601 No Difference: (Reach=R08)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.420282 0.571706 0.000240 Power (Alpha=.010) 0.166789 0.256378 0.000026 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.9977 0.087913 No Difference < 0 -1.9977 0.043957 Yes Difference > 0 -1.9977 0.956043 No Difference: (Reach=R08)-(Reach=R09) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.402243 0.558111 0.000264 Power (Alpha=.010) 0.150149 0.237398 0.000031 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.6108 0.2963 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.375235 0.601069 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R09 Count 5 5 Median 4.89 18.6 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:59 AM 233 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R09 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 4 19 21 36 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.047619 Yes 0.952381 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.7756 0.075800 No -1.7756 0.037900 Yes -1.7756 0.962100 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.6711 0.094693 No -1.6711 0.047346 Yes -1.8800 0.969949 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.800000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes Yes No Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:59 AM 234 5/1/2013 10:43:59 AM 235 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 8.384 8.303127 3.713272 Reach=R10 5 12.216 3.566887 1.59516 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean -1.925695 7.787125 95.0% UCL of Mean 18.6937 16.64487 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -3.832 6.390016 Unequal 5.43 -3.832 9.036847 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5108 Standard Error 4.041401 4.041401 95.0% LCL Difference -13.15149 -13.97928 95.0% UCL Difference 5.487487 6.315283 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.9482 0.370793 No Difference < 0 -0.9482 0.185397 No Difference > 0 -0.9482 0.814603 No Difference: (Reach=R 08)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.133637 0.218971 0.005886 Power (Alpha=.010) 0.035986 0.063446 0.000851 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.9482 0.383314 No Difference < 0 -0.9482 0.191657 No Difference > 0 -0.9482 0.808343 No Difference: (Reach=R 08)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.124105 0.208485 0.006420 Power (Alpha=.010) 0.031029 0.056255 0.001008 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.4188 0.4653 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.130502 0.514404 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R10 Count 5 5 Median 4.89 13.4 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:43:59 AM 236 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 6 21 19 34 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.111111 No 0.888889 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.3578 0.174525 No -1.3578 0.087263 No -1.3578 0.912737 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.2534 0.210075 No -1.2534 0.105038 No -1.4623 0.928164 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:43:59 AM 237 5/1/2013 10:43:59 AM 238 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 8.384 8.303127 3.713272 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean -1.925695 8.668879 95.0% UCL of Mean 18.6937 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -6.934 6.986346 Unequal 6.84 -6.934 9.880185 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3761 Standard Error 4.418553 4.418553 95.0% LCL Difference -17.1232 -17.433 95.0% UCL Difference 3.255201 3.564998 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.5693 0.155219 No Difference < 0 -1.5693 0.077609 No Difference > 0 -1.5693 0.922391 No Difference: (Reach=R08)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.282794 0.417233 0.000987 Power (Alpha=.010) 0.095718 0.156535 0.000119 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.5693 0.161586 No Difference < 0 -1.5693 0.080793 No Difference > 0 -1.5693 0.919207 No Difference: (Reach=R08)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.272689 0.408448 0.001048 Power (Alpha=.010) 0.088323 0.147323 0.000133 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.4042 0.0874 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.416366 0.775007 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R11 Count 5 5 Median 4.89 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:00 AM 239 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 6 21 19 34 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.111111 No 0.888889 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.3578 0.174525 No -1.3578 0.087263 No -1.3578 0.912737 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.2534 0.210075 No -1.2534 0.105038 No -1.4623 0.928164 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:00 AM 240 5/1/2013 10:44:00 AM 241 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 8.384 8.303127 3.713272 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean -1.925695 4.463322 95.0% UCL of Mean 18.6937 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -0.33 6.350646 Unequal 5.32 -0.33 8.98117 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5245 Standard Error 4.016501 4.016501 95.0% LCL Difference -9.592069 -10.4698 95.0% UCL Difference 8.932069 9.809797 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.0822 0.936537 No Difference < 0 -0.0822 0.468269 No Difference > 0 -0.0822 0.531731 No Difference: (Reach=R08)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.050608 0.058284 0.042680 Power (Alpha=.010) 0.010168 0.012017 0.008283 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.0822 0.937520 No Difference < 0 -0.0822 0.468760 No Difference > 0 -0.0822 0.531240 No Difference: (Reach=R08)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.050539 0.057926 0.042957 Power (Alpha=.010) 0.010139 0.011859 0.008397 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.8827 0.5029 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.114391 0.498388 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R12 Count 5 5 Median 4.89 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:00 AM 242 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 8 23 17 32 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.210318 No 0.789683 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.9400 0.347208 No -0.9400 0.173604 No -0.9400 0.826396 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.8356 0.403395 No -0.8356 0.201698 No -1.0445 0.851865 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:00 AM 243 5/1/2013 10:44:00 AM 244 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 8.384 8.303127 3.713272 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean -1.925695 7.741103 95.0% UCL of Mean 18.6937 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -1.388 5.984029 Unequal 4.31 -1.388 8.462694 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6994 Standard Error 3.784632 3.784632 95.0% LCL Difference -10.11538 -11.60439 95.0% UCL Difference 7.339377 8.828389 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.3667 0.723319 No Difference < 0 -0.3667 0.361659 No Difference > 0 -0.3667 0.638341 No Difference: (Reach=R 08)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.062182 0.095422 0.023720 Power (Alpha=.010) 0.013433 0.021922 0.004159 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.3667 0.731111 No Difference < 0 -0.3667 0.365556 No Difference > 0 -0.3667 0.634444 No Difference: (Reach=R 08)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.059913 0.091497 0.024973 Power (Alpha=.010) 0.012485 0.020008 0.004609 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 25.7701 1.3831 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.008164 0.273382 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R13 Count 5 5 Median 4.89 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:00 AM 245 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 6 21 19 34 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.111111 No 0.888889 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -1.3578 0.174525 No -1.3578 0.087263 No -1.3578 0.912737 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -1.2534 0.210075 No -1.2534 0.105038 No -1.4623 0.928164 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:00 AM 246 5/1/2013 10:44:00 AM 247 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 8.384 8.303127 3.713272 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean -1.925695 4.72547 95.0% UCL of Mean 18.6937 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -0.732 6.381429 Unequal 5.40 -0.732 9.024703 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5138 Standard Error 4.03597 4.03597 95.0% LCL Difference -10.03896 -10.87744 95.0% UCL Difference 8.574963 9.413438 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.1814 0.860589 No Difference < 0 -0.1814 0.430294 No Difference > 0 -0.1814 0.569706 No Difference: (Reach=R08)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.052965 0.069675 0.035019 Power (Alpha=.010) 0.010824 0.014911 0.006557 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.1814 0.862686 No Difference < 0 -0.1814 0.431343 No Difference > 0 -0.1814 0.568657 No Difference: (Reach=R08)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.052642 0.068801 0.035514 Power (Alpha=.010) 0.010687 0.014506 0.006752 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 5.5139 0.5183 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.126935 0.492082 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R14 Count 5 5 Median 4.89 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:00 AM 248 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 8 23 17 32 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.210318 No 0.789683 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.9400 0.347208 No -0.9400 0.173604 No -0.9400 0.826396 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.8356 0.403395 No -0.8356 0.201698 No -1.0445 0.851865 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:01 AM 249 5/1/2013 10:44:01 AM 250 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R08 5 8.384 8.303127 3.713272 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R08) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean -1.925695 5.414244 95.0% UCL of Mean 18.6937 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 0.438 6.045637 Unequal 4.48 0.438 8.549822 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6628 Standard Error 3.823597 3.823597 95.0% LCL Difference -8.37923 -9.743588 95.0% UCL Difference 9.25523 10.61959 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.1146 0.911623 No Difference < 0 0.1146 0.544188 No Difference > 0 0.1146 0.455812 No Difference: (Reach=R 08)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.051182 0.040043 0.061831 Power (Alpha=.010) 0.010328 0.007680 0.012904 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.1146 0.913753 No Difference < 0 0.1146 0.543123 No Difference > 0 0.1146 0.456877 No Difference: (Reach=R 08)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.050980 0.040604 0.061029 Power (Alpha=.010) 0.010245 0.007904 0.012552 Tests of Assumptions Section Assumption Skewness Normality (Reach=R08) Kurtosis Normality (Reach=R08) Omnibus Normality (Reach=R08) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 16.5824 1.1332 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.018673 0.318157 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R08 Reach=R15 Count 5 5 Median 4.89 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:01 AM 251 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R08 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 8 23 17 32 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.210318 No 0.789683 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.9400 0.347208 No -0.9400 0.173604 No -0.9400 0.826396 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.8356 0.403395 No -0.8356 0.201698 No -1.0445 0.851865 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.600000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:01 AM 252 5/1/2013 10:44:01 AM 253 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 22.48 13.41611 5.999866 Reach=R10 5 12.216 3.566887 1.59516 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R 10) = 2.7764 95.0% LCL of Mean 5.8217 7.787125 95.0% UCL of Mean 39.1383 16.64487 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 10.264 9.816177 Unequal 4.56 10.264 13.88217 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6465 Standard Error 6.208296 6.208296 95.0% LCL Difference -4.052356 -6.166184 95.0% UCL Difference 24.58036 26.69418 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.6533 0.136875 No Difference < 0 1.6533 0.931562 No Difference > 0 1.6533 0.068438 No Difference: (Reach=R 09)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.308052 0.000757 0.447288 Power (Alpha=.010) 0.107555 0.000089 0.173819 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.6533 0.164761 No Difference < 0 1.6533 0.917620 No Difference > 0 1.6533 0.082380 No Difference: (Reach=R 09)-(R each=R 10) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.261542 0.000985 0.406262 Power (Alpha=.010) 0.075801 0.000143 0.132934 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 14.1473 1.2422 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.024999 0.297415 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R10 Count 5 5 Median 18.6 13.4 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:01 AM 254 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R10 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 35 5 20 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.924603 No 0.075397 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.5667 0.117185 No 1.5667 0.941407 No 1.5667 0.058593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.4623 0.143672 No 1.6711 0.952654 No 1.4623 0.071836 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:01 AM 255 5/1/2013 10:44:01 AM 256 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 22.48 13.41611 5.999866 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 5.8217 8.668879 95.0% UCL of Mean 39.1383 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 7.162 10.2144 Unequal 5.24 7.162 14.44535 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5352 Standard Error 6.460156 6.460156 95.0% LCL Difference -7.735148 -9.215512 95.0% UCL Difference 22.05915 23.53951 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1086 0.299798 No Difference < 0 1.1086 0.850101 No Difference > 0 1.1086 0.149899 No Difference: (Reach=R09)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.165166 0.003823 0.264650 Power (Alpha=.010) 0.047195 0.000526 0.081921 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.1086 0.315831 No Difference < 0 1.1086 0.842084 No Difference > 0 1.1086 0.157916 No Difference: (Reach=R09)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.150519 0.004292 0.249426 Power (Alpha=.010) 0.039124 0.000656 0.070579 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 6.2767 0.7073 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.102932 0.424766 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R11 Count 5 5 Median 18.6 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:01 AM 257 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 17 32 8 23 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.789683 No 0.210318 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.9400 0.347208 No 0.9400 0.826396 No 0.9400 0.173604 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.8356 0.403395 No 1.0445 0.851865 No 0.8356 0.201698 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.000000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:01 AM 258 5/1/2013 10:44:02 AM 259 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 22.48 13.41611 5.999866 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 5.8217 4.463322 95.0% UCL of Mean 39.1383 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 13.766 9.790594 Unequal 4.52 13.766 13.84599 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6552 Standard Error 6.192116 6.192116 95.0% LCL Difference -0.5130442 -2.675064 95.0% UCL Difference 28.04504 30.20706 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2231 0.056902 No Difference < 0 2.2231 0.971549 No Difference > 0 2.2231 0.028451 Yes Difference: (Reach=R09)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.498007 0.000107 0.650009 Power (Alpha=.010) 0.214938 0.000011 0.319500 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2231 0.082556 No Difference < 0 2.2231 0.958722 No Difference > 0 2.2231 0.041278 Yes Difference: (Reach=R09)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.419475 0.000158 0.592883 Power (Alpha=.010) 0.142373 0.000021 0.236019 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 15.3584 1.2807 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.021508 0.290557 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R12 Count 5 5 Median 18.6 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:02 AM 260 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:02 AM 261 5/1/2013 10:44:02 AM 262 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 22.48 13.41611 5.999866 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 5.8217 7.741103 95.0% UCL of Mean 39.1383 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 12.708 9.556863 Unequal 4.12 12.708 13.51545 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7451 Standard Error 6.044291 6.044291 95.0% LCL Difference -1.23016 -3.884337 95.0% UCL Difference 26.64616 29.30034 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1025 0.068672 No Difference < 0 2.1025 0.965664 No Difference > 0 2.1025 0.034336 Yes Difference: (Reach=R 09)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.456214 0.000166 0.608663 Power (Alpha=.010) 0.188254 0.000017 0.284916 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1025 0.101363 No Difference < 0 2.1025 0.949319 No Difference > 0 2.1025 0.050681 No Difference: (Reach=R 09)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.368843 0.000256 0.541407 Power (Alpha=.010) 0.115523 0.000037 0.197772 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 67.2799 2.1080 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.001274 0.184594 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R13 Count 5 5 Median 18.6 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:02 AM 263 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:02 AM 264 5/1/2013 10:44:02 AM 265 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 22.48 13.41611 5.999866 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 5.8217 4.72547 95.0% UCL of Mean 39.1383 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 13.364 9.81059 Unequal 4.55 13.364 13.87427 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6484 Standard Error 6.204762 6.204762 95.0% LCL Difference -0.9442058 -3.068473 95.0% UCL Difference 27.6722 29.79647 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1538 0.063396 No Difference < 0 2.1538 0.968302 No Difference > 0 2.1538 0.031698 Yes Difference: (Reach=R09)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.473971 0.000138 0.626442 Power (Alpha=.010) 0.199358 0.000014 0.299422 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1538 0.089227 No Difference < 0 2.1538 0.955386 No Difference > 0 2.1538 0.044614 Yes Difference: (Reach=R09)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.400104 0.000199 0.571426 Power (Alpha=.010) 0.133557 0.000027 0.222705 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 14.3955 1.3006 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.024218 0.287096 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R14 Count 5 5 Median 18.6 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:02 AM 266 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R14 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 23.5 38.5 1.5 16.5 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.3048 0.021177 Yes 2.3048 0.989412 No 2.3048 0.010588 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.2001 0.027803 Yes 2.4096 0.992015 No 2.2001 0.013901 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:02 AM 267 5/1/2013 10:44:03 AM 268 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R09 5 22.48 13.41611 5.999866 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R09) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 5.8217 5.414244 95.0% UCL of Mean 39.1383 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 14.534 9.59556 Unequal 4.18 14.534 13.57017 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.7288 Standard Error 6.068765 6.068765 95.0% LCL Difference 0.5394033 -2.026326 95.0% UCL Difference 28.5286 31.09433 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3949 0.043523 Yes Difference < 0 2.3949 0.978239 No Difference > 0 2.3949 0.021761 Yes Difference: (Reach=R 09)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.557343 0.000056 0.705769 Power (Alpha=.010) 0.256346 0.000005 0.371372 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3949 0.071937 No Difference < 0 2.3949 0.964032 No Difference > 0 2.3949 0.035968 Yes Difference: (Reach=R 09)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.456117 0.000093 0.635778 Power (Alpha=.010) 0.156233 0.000013 0.258830 Tests of Assumptions Section Assumption Skewness Normality (Reach=R09) Kurtosis Normality (Reach=R09) Omnibus Normality (Reach=R09) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 43.2930 1.8947 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.003012 0.205966 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R09 Reach=R15 Count 5 5 Median 18.6 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:03 AM 269 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R09 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 25 40 0 15 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.007937 Yes 0.996032 No 0.003968 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.6112 0.009023 Yes 2.6112 0.995488 No 2.6112 0.004512 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.5067 0.012186 Yes 2.7156 0.996692 No 2.5067 0.006093 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 1.000000 0.000000 1.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0079 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:03 AM 270 50 n Channel_Catfish_PFOS_ppb vs Groups 5/1/2013 10:44:03 AM 271 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R10 5 12.216 3.566887 1.59516 Reach=R11 5 15.318 5.355009 2.394833 Note: T-alpha (Reach:=R10) = 2.7764, T-alpha (Reach=R11) = 2.7764 95.0% LCL of Mean 7.787125 8.668879 95.0% UCL of Mean 16.64487 21.96712 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -3.102 4.549659 Unequal 6.97 -3.102 6.43419 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3670 Standard Error 2.877457 2.877457 95.0% LCL Difference -9.737429 -9.912924 95.0% UCL Difference 3.533428 3.708924 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.0780 0.312447 No Difference < 0 -1.0780 0.156224 No Difference > 0 -1.0780 0.843776 No Difference: (Reach=R10)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.158756 0.255582 0.004158 Power (Alpha=.010) 0.044855 0.078119 0.000577 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -1.0780 0.316929 No Difference < 0 -1.0780 0.158465 No Difference > 0 -1.0780 0.841535 No Difference: (Reach=R10)-(Reach=R11) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.154668 0.251390 0.004297 Power (Alpha=.010) 0.042492 0.074867 0.000616 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.2539 0.5960 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.450573 0.462286 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R11 Count 5 5 Median 13.4 15.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:03 AM 272 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R11 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 8 23 17 32 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.420635 No 0.210318 No 0.789683 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.9400 0.347208 No -0.9400 0.173604 No -0.9400 0.826396 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.8356 0.403395 No -0.8356 0.201698 No -1.0445 0.851865 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.000000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:03 AM 273 24 -, Channel_Catfish_PFOS_ppb vs Groups 5/1/2013 10:44:03 AM 274 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R10 5 12.216 3.566887 1.59516 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R10) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 7.787125 4.463322 95.0% UCL of Mean 16.64487 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 3.502 3.495866 Unequal 7.99 3.502 4.943901 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3067 Standard Error 2.21098 2.21098 95.0% LCL Difference -1.596529 -1.598024 95.0% UCL Difference 8.600529 8.602024 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.5839 0.151872 No Difference < 0 1.5839 0.924064 No Difference > 0 1.5839 0.075936 No Difference: (Reach=R10)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.287118 0.000943 0.422437 Power (Alpha=.010) 0.097708 0.000113 0.159462 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.5839 0.151936 No Difference < 0 1.5839 0.924032 No Difference > 0 1.5839 0.075968 No Difference: (Reach=R10)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.287015 0.000944 0.422348 Power (Alpha=.010) 0.097629 0.000113 0.159365 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.0856 0.0093 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.938469 0.925357 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R12 Count 5 5 Median 13.4 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:03 AM 275 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 19 34 6 21 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.222222 No 0.888889 No 0.111111 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.3578 0.174525 No 1.3578 0.912737 No 1.3578 0.087263 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.2534 0.210075 No 1.4623 0.928164 No 1.2534 0.105038 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:03 AM 276 5/1/2013 10:44:04 AM 277 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R10 5 12.216 3.566887 1.59516 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (Reach:=R10) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 7.787125 7.741103 95.0% UCL of Mean 16.64487 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 2.444 2.774703 Unequal 5.61 2.444 3.924022 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4886 Standard Error 1.754876 1.754876 95.0% LCL Difference -1.602751 -1.923238 95.0% UCL Difference 6.490751 6.811238 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.3927 0.201198 No Difference < 0 1.3927 0.899401 No Difference > 0 1.3927 0.100599 No Difference: (Reach=R 10)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.233239 0.001693 0.355730 Power (Alpha=.010) 0.073973 0.000214 0.123945 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.3927 0.216390 No Difference < 0 1.3927 0.891805 No Difference > 0 1.3927 0.108195 No Difference: (Reach=R 10)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.213873 0.001919 0.337562 Power (Alpha=.010) 0.061625 0.000271 0.107661 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.7557 1.8836 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.160139 0.207170 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R13 Count 5 5 Median 13.4 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:04 AM 278 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 18 33 7 22 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.309524 No 0.845238 No 0.154762 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.1489 0.250592 No 1.1489 0.874704 No 1.1489 0.125296 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.0445 0.296270 No 1.2534 0.894962 No 1.0445 0.148135 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:04 AM 279 5/1/2013 10:44:04 AM 280 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R10 5 12.216 3.566887 1.59516 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (R e ac h =R10) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 7.787125 4.72547 95.0% UCL of Mean 16.64487 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 3.1 3.551479 Unequal 8.00 3.1 5.02255 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3060 Standard Error 2.246153 2.246153 95.0% LCL Difference -2.079638 -2.079706 95.0% UCL Difference 8.279637 8.279706 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.3801 0.204882 No Difference < 0 1.3801 0.897559 No Difference > 0 1.3801 0.102441 No Difference: (Reach=R10)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.229916 0.001757 0.351475 Power (Alpha=.010) 0.072584 0.000223 0.121821 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.3801 0.204885 No Difference < 0 1.3801 0.897558 No Difference > 0 1.3801 0.102442 No Difference: (Reach=R10)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.229912 0.001758 0.351472 Power (Alpha=.010) 0.072581 0.000223 0.121818 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.0175 0.0090 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.986957 0.926844 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R14 Count 5 5 Median 13.4 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:04 AM 281 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 20 35 5 20 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.150794 No 0.924603 No 0.075397 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.5667 0.117185 No 1.5667 0.941407 No 1.5667 0.058593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.4623 0.143672 No 1.6711 0.952654 No 1.4623 0.071836 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:04 AM 282 5/1/2013 10:44:04 AM 283 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard1 Variable Count Mean Deviation Error Reach=R10 5 12.216 3.566887 1.59516 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R10) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 7.787125 5.414244 95.0% UCL of Mean 16.64487 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 4.27 2.905186 Unequal 6.36 4.27 4.108553 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4136 Standard1 Error 1.837401 1.837401 95.0% LCL Difference 0.03294597 -0.1646654 95.0% UCL Difference 8.507054 8.704665 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3239 0.048620 Yes Difference < 0 2.3239 0.975690 No Difference > 0 2.3239 0.024310 Yes Difference: (Reach=R 10)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.532919 0.000074 0.683230 Power (Alpha=.010) 0.238768 0.000007 0.349607 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3239 0.056711 No Difference < 0 2.3239 0.971644 No Difference > 0 2.3239 0.028356 Yes Difference: (Reach=R 10)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.504514 0.000086 0.664100 Power (Alpha=.010) 0.207319 0.000010 0.316089 Tests of Assumptions Section Assumption Skewness Normality (Reach=R10) Kurtosis Normality (Reach=R10) Omnibus Normality (Reach=R10) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.0602 1.0536 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.304208 0.334720 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R10 Reach=R15 Count 5 5 Median 13.4 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:04 AM 284 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R10 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 21 36 4 19 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.952381 No 0.047619 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.7756 0.075800 No 1.7756 0.962100 No 1.7756 0.037900 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.6711 0.094693 No 1.8800 0.969949 No 1.6711 0.047346 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:04 AM 285 5/1/2013 10:44:04 AM 286 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R11 5 15.318 5.355009 2.394833 Reach=R12 5 8.714 3.423373 1.530979 Note: T-alpha (Reach:=R11) = 2.7764, T-alpha (Reach=R12) = 2.7764 95.0% LCL of Mean 8.668879 4.463322 95.0% UCL of Mean 21.96712 12.96468 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 6.604 4.494196 Unequal 6.80 6.604 6.355753 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3787 Standard Error 2.842379 2.842379 95.0% LCL Difference 0.04946161 -0.1571177 95.0% UCL Difference 13.15854 13.36512 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3234 0.048660 Yes Difference < 0 2.3234 0.975670 No Difference > 0 2.3234 0.024330 Yes Difference: (Reach=R11)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.532736 0.000074 0.683059 Power (Alpha=.010) 0.238640 0.000007 0.349447 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.3234 0.054179 No Difference < 0 2.3234 0.972911 No Difference > 0 2.3234 0.027089 Yes Difference: (Reach=R11)-(Reach=R12) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.513259 0.000082 0.670018 Power (Alpha=.010) 0.216649 0.000009 0.326222 Tests of Assumptions Section Assumption Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.4469 0.6091 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.407335 0.457608 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11 Reach=R12 Count 5 5 Median 15.7 10.7 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:04 AM 287 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11 Reach=R12 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 22 37 3 18 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.972222 No 0.027778 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.9845 0.047202 Yes 1.9845 0.976399 No 1.9845 0.023601 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.8800 0.060103 No 2.0889 0.981643 No 1.8800 0.030051 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:05 AM 288 5/1/2013 10:44:05 AM 289 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R11 5 15.318 5.355009 2.394833 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (Reach:=R11) = 2.7764, T-alpha (Reach=R13) = 2.7764 95.0% LCL of Mean 8.668879 7.741103 95.0% UCL of Mean 21.96712 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 5.546 3.959254 Unequal 4.74 5.546 5.599231 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.6136 Standard Error 2.504052 2.504052 95.0% LCL Difference -0.228355 -0.9985766 95.0% UCL Difference 11.32036 12.09058 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2148 0.057647 No Difference < 0 2.2148 0.971177 No Difference > 0 2.2148 0.028823 Yes Difference: (Reach=R11)-(Reach=R13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.495114 0.000110 0.647203 Power (Alpha=.010) 0.213028 0.000011 0.317056 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.2148 0.080592 No Difference < 0 2.2148 0.959704 No Difference > 0 2.2148 0.040296 Yes Difference: (Reach=R11)-(Reach=R13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.424985 0.000157 0.596378 Power (Alpha=.010) 0.147337 0.000021 0.241953 Tests of Assumptions Section Assumption Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 10.7190 4.0247 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.041204 0.079752 Cannot reject normality Reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11 Reach=R13 Count 5 5 Median 15.7 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:05 AM 290 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 21 36 4 19 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.095238 No 0.952381 No 0.047619 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.7756 0.075800 No 1.7756 0.962100 No 1.7756 0.037900 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.6711 0.094693 No 1.8800 0.969949 No 1.6711 0.047346 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:05 AM 291 5/1/2013 10:44:05 AM 292 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R11 5 15.318 5.355009 2.394833 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R11) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 8.668879 4.72547 95.0% UCL of Mean 21.96712 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 6.202 4.53759 Unequal 6.93 6.202 6.417121 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3694 Standard Error 2.869824 2.869824 95.0% LCL Difference -0.4158261 -0.5977862 95.0% UCL Difference 12.81983 13.00179 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1611 0.062681 No Difference < 0 2.1611 0.968659 No Difference > 0 2.1611 0.031341 Yes Difference: (Reach=R11)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.476492 0.000134 0.628942 Power (Alpha=.010) 0.200962 0.000014 0.301504 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.1611 0.067883 No Difference < 0 2.1611 0.966059 No Difference > 0 2.1611 0.033941 Yes Difference: (Reach=R11)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.460822 0.000146 0.617830 Power (Alpha=.010) 0.184867 0.000016 0.283893 Tests of Assumptions Section Assumption Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.2935 0.7069 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.441180 0.424877 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11 Reach=R14 Count 5 5 Median 15.7 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:05 AM 293 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 22 37 3 18 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.055556 No 0.972222 No 0.027778 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 1.9845 0.047202 Yes 1.9845 0.976399 No 1.9845 0.023601 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 1.8800 0.060103 No 2.0889 0.981643 No 1.8800 0.030051 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:05 AM 294 5/1/2013 10:44:05 AM 295 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R11 5 15.318 5.355009 2.394833 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R11) = 2.7764, T-alpha (Reach=R15) = 2.7764 95.0% LCL of Mean 8.668879 5.414244 95.0% UCL of Mean 21.96712 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 7.372 4.051768 Unequal 5.14 7.372 5.730065 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.5502 Standard Error 2.562563 2.562563 95.0% LCL Difference 1.462719 0.8368346 95.0% UCL Difference 13.28128 13.90717 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.8768 0.020612 Yes Difference < 0 2.8768 0.989694 No Difference > 0 2.8768 0.010306 Yes Difference: (Reach=R11)-(Reach=R15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.712878 0.000008 0.835767 Power (Alpha=.010) 0.390177 0.000001 0.525990 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 2.8768 0.033653 Yes Difference < 0 2.8768 0.983174 No Difference > 0 2.8768 0.016826 Yes Difference: (Reach=R11)-(Reach=R15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.642469 0.000012 0.796614 Power (Alpha=.010) 0.286814 0.000001 0.426238 Tests of Assumptions Section Assumption Skewness Normality (Reach=R11) Kurtosis Normality (Reach=R11) Omnibus Normality (Reach=R11) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 6.8974 2.9632 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.088081 0.123483 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R11 Reach=R15 Count 5 5 Median 15.7 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:05 AM 296 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R11 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 23 38 2 17 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.031746 Yes 0.984127 No 0.015873 Yes Approx. Without Correction Prob Reject H0 Z-Value Level at .050 2.1934 0.028280 Yes 2.1934 0.985860 No 2.1934 0.014140 Yes Approx. With Correction Prob Reject H0 Z-Value Level at .050 2.0889 0.036714 Yes 2.2978 0.989214 No 2.0889 0.018357 Yes Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.800000 0.000000 0.800000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) Yes No Yes Prob Level 0.0794 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:06 AM 297 5/1/2013 10:44:06 AM 298 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R12 5 8.714 3.423373 1.530979 Reach=R13 5 9.772 1.635625 0.7314739 Note: T-alpha (Reach:=R12) = 2.7764, T-alpha (Reach=R 13) = 2.7764 95.0% LCL of Mean 4.463322 7.741103 95.0% UCL of Mean 12.96468 11.8029 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -1.058 2.682792 Unequal 5.74 -1.058 3.794041 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4745 Standard Error 1.696747 1.696747 95.0% LCL Difference -4.970706 -5.256599 95.0% UCL Difference 2.854705 3.140599 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.6235 0.550286 No Difference < 0 -0.6235 0.275143 No Difference > 0 -0.6235 0.724857 No Difference: (Reach=R 12)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.085590 0.141700 0.013240 Power (Alpha=.010) 0.020375 0.036007 0.002127 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.6235 0.556891 No Difference < 0 -0.6235 0.278445 No Difference > 0 -0.6235 0.721555 No Difference: (Reach=R 12)-(R each=R 13) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.082274 0.137414 0.013845 Power (Alpha=.010) 0.018819 0.033512 0.002327 Tests of Assumptions Section Assumption Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.3807 1.0004 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.181565 0.346491 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R12 Reach=R13 Count 5 5 Median 10.7 10.3 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:06 AM 299 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R12 Reach=R13 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 12 27 13 28 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 1.000000 No 0.500000 No 0.500000 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.1044 0.916815 No -0.1044 0.458407 No -0.1044 0.541593 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.0000 1.000000 No 0.0000 0.500000 No -0.2089 0.582734 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:06 AM 300 5/1/2013 10:44:06 AM 301 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R12 5 8.714 3.423373 1.530979 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R12) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 4.463322 4.72547 95.0% UCL of Mean 12.96468 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 -0.402 3.480145 Unequal 7.99 -0.402 4.921668 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3064 Standard Error 2.201037 2.201037 95.0% LCL Difference -5.4776 -5.478525 95.0% UCL Difference 4.673599 4.674525 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.1826 0.859623 No Difference < 0 -0.1826 0.429812 No Difference > 0 -0.1826 0.570188 No Difference: (Reach=R12)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.053007 0.069831 0.034928 Power (Alpha=.010) 0.010836 0.014952 0.006537 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 -0.1826 0.859628 No Difference < 0 -0.1826 0.429814 No Difference > 0 -0.1826 0.570186 No Difference: (Reach=R12)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.053006 0.069829 0.034929 Power (Alpha=.010) 0.010835 0.014951 0.006537 Tests of Assumptions Section Assumption Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.0669 0.0001 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.951477 0.990701 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R12 Reach=R14 Count 5 5 Median 10.7 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:06 AM 302 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R12 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 11 26 14 29 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.841270 No 0.420635 No 0.579365 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 -0.3133 0.754023 No -0.3133 0.377011 No -0.3133 0.622989 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 -0.2089 0.834532 No -0.2089 0.417266 No -0.4178 0.661948 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.400000 0.200000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:06 AM 303 5/1/2013 10:44:06 AM 304 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R12 5 8.714 3.423373 1.530979 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R12) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 4.463322 5.414244 95.0% UCL of Mean 12.96468 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 0.768 2.817535 Unequal 6.52 0.768 3.984596 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4003 Standard Error 1.781966 1.781966 95.0% LCL Difference -3.34122 -3.509274 95.0% UCL Difference 4.877221 5.045274 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.4310 0.677857 No Difference < 0 0.4310 0.661072 No Difference > 0 0.4310 0.338928 No Difference: (Reach=R 12)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.066862 0.020598 0.105801 Power (Alpha=.010) 0.014785 0.003532 0.024920 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.4310 0.680365 No Difference < 0 0.4310 0.659817 No Difference > 0 0.4310 0.340183 No Difference: (Reach=R 12)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.065953 0.020955 0.104415 Power (Alpha=.010) 0.014372 0.003661 0.024175 Tests of Assumptions Section Assumption Skewness Normality (Reach=R12) Kurtosis Normality (Reach=R12) Omnibus Normality (Reach=R12) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 2.8189 0.5327 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.339597 0.486288 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R12 Reach=R15 Count 5 5 Median 10.7 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:06 AM 305 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R12 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 15 30 10 25 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.690476 No 0.654762 No 0.345238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.5222 0.601508 No 0.5222 0.699246 No 0.5222 0.300754 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.4178 0.676103 No 0.6267 0.734565 No 0.4178 0.338052 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.400000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:06 AM 306 5/1/2013 10:44:07 AM 307 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R13 5 9.772 1.635625 0.7314739 Reach=R14 5 9.116 3.536005 1.581349 Note: T-alpha (Reach:=R13) = 2.7764, T-alpha (Reach=R14) = 2.7764 95.0% LCL of Mean 7.741103 4.72547 95.0% UCL of Mean 11.8029 13.50653 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 0.656 2.754869 Unequal 5.64 0.656 3.895972 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4856 Standard Error 1.742332 1.742332 95.0% LCL Difference -3.361824 -3.674818 95.0% UCL Difference 4.673824 4.986818 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.3765 0.716330 No Difference < 0 0.3765 0.641835 No Difference > 0 0.3765 0.358165 No Difference: (Reach=R13)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.062843 0.023221 0.096949 Power (Alpha=.010) 0.013623 0.004058 0.022357 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.3765 0.720302 No Difference < 0 0.3765 0.639849 No Difference > 0 0.3765 0.360151 No Difference: (Reach=R13)-(Reach=R14) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.061601 0.023855 0.094886 Power (Alpha=.010) 0.013077 0.004290 0.021299 Tests of Assumptions Section Assumption Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 4.6737 1.4492 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.164489 0.263063 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13 Reach=R14 Count 5 5 Median 10.3 8.01 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:07 AM 308 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13 Reach=R14 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 15 30 10 25 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.690476 No 0.654762 No 0.345238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.5222 0.601508 No 0.5222 0.699246 No 0.5222 0.300754 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.4178 0.676103 No 0.6267 0.734565 No 0.4178 0.338052 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:07 AM 309 5/1/2013 10:44:07 AM 310 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R13 5 9.772 1.635625 0.7314739 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (R e ac h =R13) = 2.7764, T-alpha (Reach=R15) = 2.7764 95.0% LCL of Mean 7.741103 5.414244 95.0% UCL of Mean 11.8029 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 1.826 1.848351 Unequal 7.64 1.826 2.613962 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.3250 Standard Error 1.169 1.169 95.0% LCL Difference -0.8697178 -0.8919601 95.0% UCL Difference 4.521718 4.54396 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.5620 0.156908 No Difference < 0 1.5620 0.921546 No Difference > 0 1.5620 0.078454 No Difference: (Reach=R 13)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.280655 0.001010 0.414649 Power (Alpha=.010) 0.094739 0.000122 0.155093 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 1.5620 0.158675 No Difference < 0 1.5620 0.920663 No Difference > 0 1.5620 0.079337 No Difference: (Reach=R 13)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.277851 0.001027 0.412226 Power (Alpha=.010) 0.092643 0.000126 0.152507 Tests of Assumptions Section Assumption Skewness Normality (Reach=R13) Kurtosis Normality (Reach=R13) Omnibus Normality (Reach=R13) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 1.5541 0.2221 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.679705 0.650037 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R13 Reach=R15 Count 5 5 Median 10.3 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:07 AM 311 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R13 Reach=R15 Number Sets of Ties = 1, Mann W Whitney U Sum Ranks 19.5 34.5 5.5 20.5 Multiplicity Factor = 6 Mean of W 27.5 27.5 Std Dev of W 4.772607 4.772607 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 Approx. Without Correction Approx. With Correction Prob Reject H0 Prob Reject H0 Z-Value Level at .050 Z-Value Level at .050 1.4667 0.142457 No 1.3619 0.173217 No 1.4667 0.928772 No 1.5715 0.941963 No 1.4667 0.071228 No 1.3619 0.086609 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.600000 0.000000 0.600000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.3571 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:07 AM 312 5/1/2013 10:44:07 AM 313 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Descriptive Statistics Section Standard Standard Variable Count Mean Deviation Error Reach=R14 5 9.116 3.536005 1.581349 Reach=R15 5 7.946 2.039002 0.9118695 Note: T-alpha (Reach:=R14) = 2.7764, T-alpha (Reach=R 15) = 2.7764 95.0% LCL of Mean 4.72547 5.414244 95.0% UCL of Mean 13.50653 10.47776 Confidence-Limits of Difference Section Variance Mean Standard Assumption DF Difference Deviation Equal 8 1.17 2.886248 Unequal 6.40 1.17 4.081772 Note: T-alpha (Equal) = 2.3060, T-alpha (Unequal) = 2.4107 Standard Error 1.825424 1.825424 95.0% LCL Difference -3.039435 -3.230576 95.0% UCL Difference 5.379435 5.570576 Equal-Variance T-Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6409 0.539480 No Difference < 0 0.6409 0.730260 No Difference > 0 0.6409 0.269740 No Difference: (Reach=R 14)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.087636 0.012703 0.145307 Power (Alpha=.010) 0.021003 0.002029 0.037181 Aspin-Welch Unequal-Variance Test Section Alternative Prob Reject H0 Hypothesis T-Value Level at .050 Difference <> 0 0.6409 0.543830 No Difference < 0 0.6409 0.728085 No Difference > 0 0.6409 0.271915 No Difference: (Reach=R 14)-(R each=R 15) The randomization test results are based on 1000 Monte Carlo samples. Power (Alpha=.050) 0.085360 0.013086 0.142426 Power (Alpha=.010) 0.019909 0.002156 0.035458 Tests of Assumptions Section Assumption Skewness Normality (Reach=R14) Kurtosis Normality (Reach=R14) Omnibus Normality (Reach=R14) Skewness Normality (Reach=R15) Kurtosis Normality (Reach=R15) Omnibus Normality (Reach=R15) Variance-Ratio Equal-Variance Test Modified-Levene Equal-Variance Test Value 0.0000 0.0000 3.0074 0.7669 Probability Decision(.050) 1.000000 Cannot reject normality 1.000000 0.311463 0.406696 Cannot reject normality Cannot reject equal variances Cannot reject equal variances Median Statistics Variable Reach=R14 Reach=R15 Count 5 5 Median 8.01 7.33 95.0% LCL 95.0% UCL of Median of Median 5/1/2013 10:44:07 AM 314 Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb Mann-Whitney U or Wilcoxon Rank-Sum Test for Difference in Medians Variable Reach=R14 Reach=R15 Number Sets of Ties = 0, Mann W Whitney U Sum Ranks 15 30 10 25 Multiplicity Factor = 0 Mean of W 27.5 27.5 Std Dev of W 4.787136 4.787136 Alternative Hypothesis Diff<>0 Diff<0 Diff>0 Exact Probability Prob Reject H0 Level at .050 0.690476 No 0.654762 No 0.345238 No Approx. Without Correction Prob Reject H0 Z-Value Level at .050 0.5222 0.601508 No 0.5222 0.699246 No 0.5222 0.300754 No Approx. With Correction Prob Reject H0 Z-Value Level at .050 0.4178 0.676103 No 0.6267 0.734565 No 0.4178 0.338052 No Kolmogorov-Smirnov Test For Different Distributions Alternative Hypothesis D(1)<>D(2) D(1)<D(2) D(1)>D(2) Dmn Criterion Value 0.400000 0.200000 0.400000 Reject H0 if Greater Than 0.7490 0.7490 0.7490 Test Alpha Level 0.050 0.025 0.025 Reject H0 (Test Alpha) No No No Prob Level 0.8730 Plots Section Dataset Variable Two-Sample Test Report \...\Data for plots\NCSS_stats\catfish_Nov_2012.NCSS Channel_Catfish_PFOS_ppb 5/1/2013 10:44:07 AM 315