Document zON58O7EKK4Rb4y09rbeRd03

AROCLOK ENVIRONMENTAL PROGRAM EUROPEAN TRIP - MAV 1-?], 1970 _Surmiary_o_f _Ajial yt_l caj _ F3_rd_i ngs 3. No significant advances have been made in methodology in Europe and the UK the past year for the Identification and measurement of PCB's. One nev; approach involves use of thin layer chi'omatography at Folkhalsan in Sweden prior to gas chromatography to get free of interferences and obtain increased specificity, A technique involving extraction with cold acetone for isolation of PCB's from tissue may shorten our tissue analysis program markedly. 2. No new analytical workers with the exception of Wiggins -Toape in the UK and Folkhalsan in Sweden have become involved in Europe during the past year. 3. Competitive companies in Germany (Bayer), France (irodelcc) and Italy are about where Monsanto was one year ago on methodology. Gas chromatography equipment suitable for this work has been purchased and will be installed in Germany and France within 1 to 3 months. Monsanto methodology at Ruabon and more recently at Newport is based on work in St. Louis. Ruabon has yet to look at water from estuaries and the sea a^id wildlife for PCB's. MONS 212475 Professor Widmavk who first Identified PCB's at the institute of Analytical Cheinj stry in Sweden now has a secondary role on PCB's. His prime interest is long range fundamental computerized mass spectrometry methodology. 6. Biodegradation facilities and supporting methodology at Ruabon is no better able to carry out degradation studies of Aroclor products, and mono isopropyl biphenyl than St, Louis. 7. Ruabon theory says that the highest chlorinated biphenyl that will degrade Is Cl Cl Cl Cl Cl Other structures that will degrade will be isomers produced by taking chlorines from this structure. It has also been postulated at the pest Infestation Control Laboratory in the UK that chlorinated biphenyls should be biodegradable if a CHs group Is present on the ring. 8. An organism which grows on PCB's has been found at the University of Bangor. A grant to continue this work has been made by Monsanto (Ruabon). 9. PCB's continue to be found in a variety of fish, mussels, birds, margarine, vegetable oils and foods of animal HONS 212476 -J- origin the UK and Europe. PC)3 residues are most typical of Monsanto Aroclor (March publication in Statens Instltut for Folkhalsan, "Levels of Organochlorinc pesticides and Polyehlorinalcd Biphenyls in Margarine, Vegetable Oils and Some Foods of Animal Origin on the Swedish Market -1967-3 969", Gunnel Westoo. Koictu Noren anc1 Margit Anderson. 3 0. PCR's continue to be found in the environment - both in fresh and marine waters and sludre from sewage treatment plants. 11. Chlorinated terphenyls have been identified in herring by Unilever in the UK and presumably by the Kolkhalean Institute in Sweden. IP. PCB's found in dead seals in the UK and birds from the Irish Sea Incident are not sufficiently high compared to that in healthy specimens to cause death. Ij5. I'CB's found in nuts and many other foods In the UK have been traced to Littlejohn packaging drums manufactured from NCR broke paper containing Aroclor 1PJ(2 sold by Wiggins Teape. 1J1. The lack of suitable known isomers continues to slow down Monsanto and outside laboratories carrying out analytical, biodegradation and toxicological studies. HONS 212477 -'1 33. PCB's were found in fish in the waters of Scotland hack to 3965 us shown by examination of o3d GC curves. No curves available for earlier years. 16. hose than 1# of fish from east and v:cst coast of Scotland contain more than 0.1 ppm PCD with a 0.03 ppm detection limj t. 17. PCB's In Scotland appear to be discharged in sewage trade wastes - hundreds of ppm found in sewage sludge - not in effluent or liquors. No problem with fresh water - appears to be marine environment only. 18. The possibility of POP'S being a contaminant in pesticides appears to be ruled out. Reason - in Scotland the TCP's arc not always fellow travelers with pesticide residues. Comment was made that only two chlorinated compounds occo" in nature. 19. PCB residue results for 5-7n Rainbow Trout exposed to 0,1 ppm Aroclor 125-1 were inconclusive. Dead fish after 121 hours contained 15-56 ppm compared to 12-70 ppm for fish which lived. ?0, Rf'Pults of fish studies indicate no biological Isomeric change In Aroc-lor 125*1 per GC curves. HONS 212478 ?1, Beef, wutton and butler from New Zealand shows no )'CB compared to that from the UK, ?2, Work is underway at Utrecht on the GC separation and identification of toxic components In pCB's. No GC peaks have yet been found for toxic components (furans) in Aroclo1* p^oducte. Study v;ill be extended to looking for furans in seals and wildlife. 22. General comments faiggesl that Bayer inay be aggressive on substitute products - we were asked if we had considered fluorinated biphenyls. 2Jl, Observations were made that the isomeric composition of Aroclor 125>JI changes upon standing in air - presumably due to volatility. ?5. A two year study at Monks Wood in England shows that fresh water fish feeding birds contain more FCB than mammal feeding birds. Insect feeders contain the lowest level. {July publication In Environmental Control {?), "Polychlorinated Biphenyls in Wild Birds in Britain and Their Avian Toxicity" by lan Prestt, D. J. Jefferies and N. V/. Moore), 26. Above workers conclude off the record that thin egg shell problem in Britain is o'ue to pesticides and not PCB's. R. E. Keller MOMS 212*79 May 2?, 1970 AROCLOR ENVIRONMENTAL PROGRAM DHTAJ LpD_REPORT ON EUROPEAN TRIP COVERING ANALYTICAL PROGRAMS This; report summerizes findings and lists actions planned for world wide Arcelor analytical projects. It covers visits made by Messrs. Papacjcorcjc, Wheeler ami the writer from May 1 through May 20. It is intended that this report will supplement toxicity, manufacturing and business oriented reports provided by Wheeler and/or Papageorgc. MAY 4_- MONSANTQ_ COMPANY , NEWPORT, WALES Findings 1. Newport completed the installation of a Hewlett-Packard 402 gas chromatograph with electron capture detector in late April. The analysis of water and mud from the River Severn for PCU's is underlay. Early GC data provided by Ruabon show 320 to 2068 ppb PCB in mud from the River Severn around the Newport area. . 2. Recovered UC1 from Newport operations contains no detectable { <6 ppb) POP. Food grade fumaric acid has not been made from UC1 for 18 months. 3. Aroclor still bottoms (9600 pounds per year) and filter cake (3600 pounds per year) have been dumped at the Pontypridd commer cial land fill disposal site. Analysis of site pools and streams showed less than 4 ppb PCD. 4. At the beginning of 1970, 30 pounds of PCS in one million gallons of effluent were produced by the plant at Newport. The current target is 0.1 pounds per day. No fish from the water around the Nowport plant site have been analyzed for PCB. The Ministry of Agriculture has requested that this be carried out. 5. No work has been done on the analysis of air samples from the Pi ant site.Analytical work was carried out to support incinera tor studies carried out by Pcrle Brothers, No PCB's were detected in scrubber tower samples. 6. Currently, there are two analysts handling 14 samples per clay for PCB analytical work at Newport. 7. A Hewlett-Packard automatic sampler for gas chromatographic work was observed in operation for 4-nitrodiphenylamine analysis. This unit, which has been in operation 3 months, is the first one in the United Kingdom. Actions 1. Notify Pal Reagen about recommendation on how to estimate PCB levels, to choose correct sample size And avoid repetitive tim-~ consuming GC runs. HONS 212480 -2- 2. Kxchangc information with Pat Reagen - Doug Casey on use of the Hewlett-Packard automatic GC sampler. MnY_ F - MONSANTO COMPANY, RUABON_,.WALKS 1. G. Telling of Unilever Colworth in the United Kingdom has observed chlorinated terphonyls and reported same along with PCIJ's. Telling is scheduled to visit the Applied Sciences Section in St. Louis on Tuesday, June 2nd, Unilever is the biggest processor of smoked herring in the United Kingdom. Dr, Tolling has found PCH's in herring. 2. Comments were made that the Irish Sea incident involving dead birds and the dead seel incident off the coast of the United Kingdom will bo covered by reports showing r.o apparent correla tion with PCB residue levels. Starvation is the apparent cause of death. PCP's found resembled the Aroclor 1254 most closely. 3. Cashew nuts ir. Littlejohn drums were found to contain PCB typical of Aroclor 1242. Analysis of the paperboard in the drums con firmed that it contains PCB. Further work showed that the PCB comes from mill broke which is excess paper material front NCR paper runs. All analytical work done at Ruabon has involved Aroclor 1242 using packed GC columns and methodology developed in St. Louis. No new methods have been developed at Ruabon. Current work is directed toward alternate columns which will be prepared by Ruabon as less costly columns for GC` work. Ruabon has yet to look at water from estuaries and rivers in the United Kingdom and no work has been done on the determination on l'CB residues in wildlife. Biodegradation work underway at Ruabon was reviewed. Results indicate that degradation occurs at the 2 and 3 chlorine positions on the biphenyl rings. By theory, it is believed that the following chlorinated biphenyl is the highest chlorinated species that will degrade. Cl Cl Cl Other structures will be isomers produced by taking chlorines from the above structure. The monitoring of degradation work is difficult because the identity of GC peaks has not been made for absolute interpretation of the data. River die-away and two liter send-continuous sludge laboratory units are in operation. 4. Ruabon has no other biodegradation data on organic compounds relative to the Aroclor environmental project. It was agreed that standard biodegradable procedures for industry use still need to be resolved. ' HONS 212481 -3- 5, a comparison of available PCB isomers both in St. Louis and Ruabon was made. Ruabon has the 2,2', 4,4', 6,6' hcxachloro*biphony] isomer available. A project which provides a grant of about $2500 for the University of Bangor was approved for the study of organisms which will grow on PCB's, Ac t i on s 1. Prepare agenda for G. Telling's visit on June 2nd. 2. Determine applicability of PCB methodology for chlorinated toiphenyls with reference to oral reports by Telling of Unilever to Ruabon. 3. Determine if St. Reagis Paper Company has NCR broke which is made into cardboard containers for the food industry. Obtain and analyze such containers for TCB. 4. Carry out biodegradation program on JIB 40 and mono isopiopyl biphenyl. Maintain close liaison with Ruabon on IIB 40 bio degradation studies which will also be carried out at Ruabon. 5. Firm up isomer availability list between Ruabon and St. Louis and exchange needed isomers. NAy 6 DEPARTMENT OF AGRICULTURE AND FISHERIES, SCOTLAND " " FRESH WATER FISHERIES, PITLOCHRY, SCOTLAND ~ Findings 1. The Pitlochry Laboratory is set up with GC electron capture instruments and has no mass spectrometer. The quality of the work done is considered high. 2. PCB1s found in fish, birds and seals qppear most typical of Aroclor 1254. 3. The laboratory is making a big effort to trace the source of pen's especially on the analysis of sewage treatment plants effluents and sludge for PCB's. It was observed that the largest Aroclor 1254 GC peak disappears regularly in fish and seals. Comments were made that the Laboratory of the Government Chemist in London could not confirm these observations. The chromatograms are very poor. 4. Comments were made that the seal problem was out of proportion on the seriousness because the PCB levels were not higher in normal coals. 5. A review of chromatograms at Pitlochry back to 1963 shows evidence of PCB's back to this date. 6. The laboratory routinely analyzes herring, mackerel, whiting, cod and similar fish from east and west coast locations for PCB's and pesticides. Less than about 1% contain more than 0.1 ppm PCB's with a detection limit of 0.05 ppm. HONS 2L24B2 -4- 7. Results from analytical programs show lower pesticide levels in estuary water from agricultural areas than from industrial areas. This is the reverse of expectations, 8. The laboratory is not really concerned about PCB's, per se, but is very concerned about how PCH's get through the water sources - Direct discharge into sewage treatment wastes seems to be the route. It has been observed that PCB's are not found in sov?ago effluent or liquors - only the sludge. Hundreds of ppm apparent Aroclor 1254 have been found in the sludge. The pesticide levels run lower. The sludge is discharged at sea. There is no problem with fresh water sources - it appears to be concentrated with the marine environment only. 9. PCB`s arc not always fellow travelers with pesticides. Conclu sion - PCB is not a likely contaminate at very low levels in pesticide products. 10. The comment was made that only two chlorinated compounds occur in nature. 11. The results of a fish toxicity study using Aroclor 1254 and rainbow trout were discussed. A solution of water containing 0.1 ppm Aroclor 1254 was prepared using 10 milliliters of 40 ppm Aroclor 1254 in acetone, The level of Aroclor was cheeked every two hours by GC. More than 1/4 of the amount of the Aroclor disappeared in 24 hours. A new solution of freshly prepared Aroclor 1254 and water was recharged every 24 hours. Twenty fish, size 5" to 7" in length, were put in the above water solution. Results showed that 7 of the fish died after 121 hours with levels ranging from 15 to 18 ppm PCH in the muscle and 45 to 56 ppm in the viscera in the dead fish. Remaining fish which lived 192 hours contained 12 to 14 ppm PCB's in the muscle and 64 to 70 ppm in the viscera. This report was qualified with the comment that Whitehall Laboratories took much longer to kill the fish under similar conditions. They made no analyses of the fish for PCH residues. 12. KxwniijEtion of the chromatograms from the fish toxicity study show no evidence of degradation of Aroclor 1254. This suggests no isomer change in this one biological system. 13. The Pitlochry Laboratory uses hexane soxhlet extraction of fish made up with sodium sulfate to obtain 100% recovery. Actj ons 1, Investigate the need for soxhlet extraction for complete recovery from PCH from tissues compared to our present cold extraction method. MOMS 212483 may By WTGGINS TEAPK/A. D. LITTLE, LONDON, ENG].AND Fi nding_s 1, Wiggins Teape nummarized analytical data obtained for Littlejohn cardboard drum1; which showed the presence of Aroclor materials in the cardboard drums and products therein, Aroclor was found in products such os bicarbonate of soda, crcain of tartar, rage, white pepper and salted peanuts, A summary of these findings is attached. 2. The availability of pure isomers for toxicity work and metabolic studies was discussed. We emphaEizod the difficulty in prepar ing sufficient amounts of isomeric materials of this type. Actions 1. Confirm the presence of PCB in food products in the United States in containers known to be packaged in containers manufactured with paper or cardboard containing PCB products. MfiY 8_-- _Tfl K LABORATORY OF GOVERNMENT CHEMISTS, LONDON, PNG LAND Find 3 ngs ]. The Government Laboratory still finds PCB's in wildlife specimens from the United Kingdom. PCB's are occasionally found in cow's milk at very low levels. PCB's ore sometimes found in beef- end mutton fat. There are a few indications of PCB at the 0.1 ppm level typical of Aroclor 1254 or 1260 in human fat. 2. Terrestrial birds of prey and herrings show the highest PCB levels. Herring liver has been found to contain as high as 100 ppm. 3. The Laboratory of the Government Chemist is supportive to other laboratories of the government. For this reason, the total project was not always understood and results found could not bo used to make conclusions. 4. Beef kidney fat, mutton kidney fat, and butter from New Zealand show no evidence of PCB's. Findings reported arc for samples from the United Kingdom. MAY 11 - INSTITUTE' OF VETERINARY PHARMACOLOGY AND TOXICOLOGY, UTRECHT, ` " " NETHERLANDS ~ "................................. " * " "' Findings . 1. Chlophan A-60 was fractionated to obtain quantities of toxic materials. Components separated by GC were identified by mass as furan type materials. Aroclor products showed no peaks which could be correlated with toxic components as determined by testing with chicken eggs. The question of generation of dioxin materials from the burning or pyrolysis of wood Containing pentachlorophenol was raised. This institute is interested in carrying out such HONS 21484 -6- 1. (Continued) experiments if this project has not already been scheduled. It was also agreed that we would provide dioxin reference materials for their use when available. 2. This institute is now looking for furans of the type that would be in polychlorinated biphenyls in seals and wildlife. 3. The need for isomeric materials for reference purposes for the study and analyses of Aroclor products was raised. It was revealed tnat synthesis work on some of the major isomers is underway at this institute. The parties responsible for this work vi 11 contact the writer in the interest of exchanging reference materials to provide a more complete set of standards. Action^ 1. Correspond with this institute and arrange for exchange of reference isomers as standards for methodology purposes. 2. Follow up on exchange of wildlife materials or extracts thereof to provide information obtained in our own laboratory as to the level of l'CB's in wildlife. HAY_ ] 2 - THE STATENS INSTITUT FOR FOLKHAJ.SAM, STOCKHOLM Findings 3. Dr. Wostoo reported on levels of organo chlorine pesticides and polychlorinated biphenyls determined in about 1400 samples of margarine, vegetable oils and foods of animal origin from 1967 thru 1969. About 901 of the food samples investigated contained at most 0.1 milligram per kilogram of PCB, The attached English summary and tables are from a March, 1970 publication covering this work. ' 2. Dr. Wesloo made a plea for standard isomeric components to obtain better analytical data for the PCD products. 3. It was apparent that Dr, Jensen was behind the scenes in this work in that the reference PCB materials for the analytical work was provided as synthetic mixtures of PCB isomers by Dr. Jensen. No mass work was done by Dr. Westoo to confirm the chroma togran.s, 4. Chromatograms provided by Dr. Westoo were reported as those for Aroclor >460. Dr. Westoo apparently was not aware that 5460 is a chlorinated terphenyl product. A copy of the chromatogram and the solid Aroclor product used was obtained and brought bock for confirmation. Little work has been done in our laboratories to date on the determination of the chlorinated terphenyls in natural products, 5. The conclusion of the report mentioned .above is that there is not too much PCR's in food stuffs, however, there still has to be tm effort to get less in the environment. Fish and meat for human HONS 212485 -7- 5. (Continued) consumption are the greatest problem areas for PCB's. Fish fiom the Baltic flea has the highest level. This laboratory has written to several government authorities about the PCH problem. It has the power to recommend only and not to enforce. 6. The food materials reported were obtained locally in Stockholm. A task group is still in operation on the PCH problem. Actions 1. Chromatograph the Aroclor 5460 used at the institute and determine methodology needed to measure the chlorinated terphenyl components. Evaluate the data reported from results obtained in our laboratory. KAY 17 - INSTITUTE OF ANALYTICAL CHEMISTRY, UNIVERSITY OP flTOCKHOT.M 1. Professor Widmark commented that about 1500 routine analyses have been turned out by the institute during the past year to provide financial support. Without this activity the institute would have undergone financial ruin, according to Widmark. 2. Widmark is currently removed from the PCB picture by his Inactivity during the past year on problems of this type. His current inter est is more on fundamental research on 10 year programs directed toward data acquisition and computerized mass spectrometry. A major portion of this effort is being supported by the tobacco institutions with work directed.toward flavor and aroma components. 3. Widmark is especially interested in more complete separations of components such as isomers in our PCB products and smoke by glass, capillary columns. It was suggested that we would arrange for him to talk with our experts in St. Louis when he visits us on June 3. 4. Widmark could not report results of his analyses because of confidentiality requested by the submitters. He continues to believe that more positive identification is needed on the PCB problem. The treatment of samples for removal of interfering components needs additional attention. 5. Widmark commented that the buyer is apparently on substitute products. No details about type or direction of their program was mentioned. 6. It was agreed that Professor Widmark will give a seminar in St. Louis on June 3 entitled, "Identification of Unknowns Using Gas Chromatography - Computerized Mass Spectrometry". Actions 1. Arrange details for Professor Widmark's visit on June 3 and set up agenda. MONS 212486 -8- MAY 13 -_I.TMEI10]jMnNS, STOCKHOLM F_indi_ng_s 1, The state of the analytical program was discussed in general witn this customer. A tour of the manufacturing facility was made with a special reference to the handling and disposal of Aroclor usod in their electrical products. KAY 14 - BKtJSSShS _Mn_ETING WITH PAY EH, PROPHLEC AND CAFF'ARO Findings 1. Manufacturers in Germany/ France and Italy are equipment-wise and method-wise at the point where we were one year ago. Bayer and Prodclcc plan to start up GC equipment which is on order within one month. Bayer reported that residue work and toxicity work with fish and residues related thereto has been delayed some months. 1-ong term feeding of mammals has been started. Actions 1. Our methodology package will be sent to these laboratories via Hon Lidgett within seven days after the meeting. MAY 15 - BAYER, LKVERSKUSEN, GERMANY Findings 1. After visiting the Bayer incineration plant/ a visit was held with Dr. Rickcrt who is head of the organic analytical laboratory. This operation is in an 8 story building and consists of 450 employees carrying out analytical work on Bayer's organic products. MAY 18 - MINISTRY OF AGRICULTURE, FISHERIES AND_FOOD,_ BURN); AM JON C~ROUCH',~ ENGLAND ` " - ---- Kindings 1. This laboratory plays a coordinating role to determine primatily how PCB's get into the environment. They monitor fish, food and sludge products. They overlap with the activities of Dr. Holden at the Department of Agriculture and Fisheries in Scotland. This laboratory has examined fish from-the west and east coasts of the United Kingdom. Also, it has looked at birds in the United Kingdom and the mortality of same is one of their problems. 2. The significance of PCB in marine organisms is under study. Cur rent findings suggest that the PCB problem is a marine problem and not a fresh water problem. Chromatograms for fish, shrimp, etc., resemble 54% chlorinated biphenyls. How widespread the problem is for PCB's in non-industrial areas is yet to be deter mined. It appears to be associated with industrial areas. A forward program of this laboratory will be to look at fish from non-polychlori mated biphenyl areas. Th'ey will be monitoring industrial estuaries for PCB and pesticides. Muscles from the Bristol channel will be examined. HONS 212487 -9- Act_ions 1. Fish samples from the Bristol area will be exchanged with Monsanto via Bon Lidgett for determination of PCB's. 2, Methodology for PCB's will be forwarded to this laboratory via Bon Lidgett. MAY 19 TillJ NATUB^CONVnRSARY MONKSWOOD EXPERIMENTAL STATION, ; HUNTINGTON ~ ' ~" Kindings 1. The Monkswood laboratory concentrates it's efforts primarily on the study of the effect of pesticides upon wildlife. This includes birds of prey and sea birds, They are currently interested in PCB's in Guillimots. They have analysed the livers of hawks, owls and herons for pesticides at the rate of 250 samples per year since 3963. Results for 559 wildlife species of birds which were analyzed from April 3966 to 1968 for PCD is being'published in the July issue of Environmental Controlt?). This article in entitled "Polychlorinated Biphenyls in Britiah and Their Avian Toxicity", by Ian Prcstt, D. J. Jeffries and N, W, Moore. The summary page from this publication is attached. The objective of this study was to categorize PCB levels geographi cally and by feeding habits of birds. The highest levels of PCB's ranging from 6 to 900 ppm in the liver were found in fresh water fish feeding birds. The high level in fresh water fish feeding birds is a surprise. The level in hawks, falcons and mammal feeding birds was the next highest. The insect, feeding birds contain the lowest level of PCB ranging from 0 to 1 ppm. The mammal feeding birds contain from 0 to 50 ppm PCB. Work will continue on analysis of birds from the United Kingdom for some years to come. The levels of PCB in heron eggs arc lower than DDT and Deoldrin. The PCB found in birds at this laboratory resemble our Aroclor 1254 product. 2. Bengalese Finches toxicity studies were carried out with Aroclor 1254. Seven birds that died after 56 days contained 70 to 697 ppm PCB in the liverB, The 20 birds used for the study contained 3 to 634 ppm in the livers. Results needed for correlation with death are inconclusive. 3. No work has been done in this laboratory on fish analysis. 4. Chromatographic data from the bird studies shows no evidence of isomeric change in residues from the Aroclor 1254. 5. Isomeric changes were observed from Aroclor 1254 after standing in air presumably caused by volatility losses. 6. Experimental work with insects (butterflies) showed no longevity or reproducible effects with near lethal doses of Aroclor 1242. No PCB residues (less than 0.02 ppm) were found. This correlates with the low residue level in birds which are insect feeders. MOMS 212488 -10- 7. This laboratory lias shifted future emphasis from lethal to sub-lethal PCD studies. 8. The Laboratory of the Government Chemist carried out the gas chromatographic analyses for the publication mentioned above. Karlier comments lead to questions about the accuracy and com petency of this laboratory. The findings reported in the above publication will be no better than the quality of data provided. 9. The people at Monkswood are confident that DDT and DDD are responsible for thin egg shell effects in Britian and not the PCB 1 B. Action s 1, Increased emphasis should be put on our Bio-Test tissue analysis program to correlate residue levels with toxicity data. These workers feel this is a very important piece of information for the total program. MAYMINISTRY OF AGRICULTURE, FISHERIES AND FOOD - PEST "INFES'TATXDN "CONTROL LABORA Tony , '"lOLWORTIl , ENGLAND ' Findings 1. This laboratory became involved with PCB's through the Irish Sea incident. The investigation was primarily analytical. 2. A mass spectrometer has been purchased to be delivered in six months for more sophisticated studies of pesticides and PCh ma terials. 3. This laboratory is getting geared to do more sophisticated metabolic studies involving chlorinated materials including PCB's with known isomers. 4. A survey has been made and work started on the preparation of known PC'B isomers for correlation of alteration of biological conditions with structure. Three to five gram quantities will be prepared. Specific isomers have not been selected for this synthesis work. 5. This laboratory has not been able to obtain the same answers for l'CR's and birds as reported by the Laboratory of the Government Chemist. They have not seen chromatograms from the Government Chemist and commented that recent personnel changes have been made with less competent people involved. 6. The finding of PCB in cashew nuts was reported on with subsequent identification in packaging materials (Littlejohn drums). This observation of PpB in food packaging materials has been prepared as a note to the editor for publication within the next several weeks in one of the technical journals. This note is attached. 7. This laboratory uses cold acetone to extract PCB from tissue which reduces fat interferences. Comments were made that this could shorten methodology time considerably. HONS 212489 -n~ 8. Comment was made that chlorinated bxphenyl containing a methyl group should be considerably more biodegradable. Copies of methodology have since been forwarded to the writer in St. Louis. Actions ] . Evaluate cold acetone extraction as an improved shortened analytical method for analysis of 1000 cubic meters of tissue in fit. Louis. Significant reduction in aiitilysis time can mean thouFsands of dollars saved on this part of the program. R. E. idler db HONS 212490 . ESJI MAHON Of AROCLOR id J^ r/ y. i. o. RusLoline ) ) Aroclor delected, but not b. Bicaibonale of Soda ) estimaled quanliaiivcly. c. Cream of Tailor ) !!. d. Soge % (w/w) 0.03 ' e. Nappy Cleanser (Boots) tract' f. Ground White Pepper (Pine Faie) 0.003 0* h. I. m. j. Ic. Bicorbonote of Soda (Poaice-Duff) Bouquet of Gomis (Twin Trees) Cream of Tartar (Pearce-Duff) Mint (Pcorce-Duff) Italian Parmesan (Gervais) 0.003 0.007 0.007 trace trace I. Pepper (Dysons) tiace E!. m, Salted Peanut* n. Smarties Sweets 0.0013 nil \ \\ ' . . * lJ o ' A, t. ;rv-v-iOs jv-- MOWS 212491 SUMMARY Of AROCLOR AND HR 40 S1UDY J f 1. Collected information on method ovoiloblc for detection of Arocloi in tissue cmd inanimate motoricils. ?. Prepaied GLC columns ond mod'fied methods to suit current problem. This involved considerable detailed siudics to determine the limit of the levels of detection. 3. TLC studies were also undcitoken but do not appsor to be sufficiently sensitive at the levels envisaged. 4. HB 40 studies have olso been initiated os for Arocloi. Howevci, the election coptuio detector is not so sensitive to this product to that a Flame Ionisation detector has been used to date. 5. Aioclor in o rot (LD 50 study 25 ml</kg) was readily detected on cxtiaction by the appiopriafe technique followed by GIC. This showed thot there wos approximately 40 mg% (i.c. per 100 g) in wet weight liver tissues. .6 HB 40 in o rat (LD 50 study 64 ml</kg) v/os baiely detectable using the Gl C/FID detection. This thowed that there was approximately 70 mg% (I.e. per 100 g.) tn wet weight liver tissues. 7. Aroclor lias been readily detected In most Littlejohn Cardboard Drums examined namely: I. Ruskolina;Bicorbonote of Soda, Cream of 1 cui:u purchased in Edinburgh. ti. Wiggins Teape samples of Cardboard; Sage; Bools Nappy Cleanser; Fine Fore, Ground While Pepper; Pcorce-Duff Bicaibanatc of Soda; Twin Trees Bouquet Garnis ond Cream of Torior (Pcarcc-Duffs). * ili. Dried Herbs - Mint (trace) Pearce- Duffs; Gervais Italian Parmesan (trace) and Dyson's Pcppi r (tra 6. Aroclor was also readily detected in Sailed Peanuts. Aroclor was not detected in Smorties Sweets. HONS 212492 0isj^cus, h ~*t Zr^lc^t t tO'U). ^ 9-0-^JU, / r' A VV^ 2*''^ Q-- --'C v Vw jT_ vv/^ .iiirr/nv i -bo c.".o inti of jwJ^oiaor.luiVjl biphenyl (I'Ci ) oori.ounlo ;n'03O.it ,`n i.c livers ; '.' t'" ,o cf i/i'i/ blr'r. la '.'rltrin varo doicmJ *'Cd by f,v,-li':u;.'. chro '.lopvpr . loot or tho yjrj apocino/in crjio fron prn'ltory bird:. bot'.,v >.i .'yril 19iG an' Artist 19Gn, 1 A \ *,;a:i found in torror/JV*i".l cpc-iioo Am iioc t ryAono of Aritn l:i, in nil th* vinbird oy^o c:t',-,ii*\od froei one :;c ,t c ,,l im' ti/o or,.':i count colOiiios rj*.in r.i>:;t o' tho JVoo'flMitoi' :;>c5ei col .oe -i' iYon -lo-rA'.l'jidn, ot.it and routh of flic hijiicot Mv : roe*. :> , four/ *M j'i'co'r.v.itor fish-foodi, iy ir'n (up to cn. 900 ypn) c.m*. bird* foo'An ; ' ruptcro (up to 70 ppn). Viio levelo ;,roco.il r.ro eimlur to thoon, of ?p*~d A, An inleutior. of Lho r.vioji taAclty o" ouo l'C?. vtr.3 obtained by foj.'inj . .rcilc. 129*; V> Dtajo.lt:r,o 1'inol.oc* Vho c..ldr.itc' dor,:: rato for >0 nortsJAty nl JJG finyc np/];j/doj* At thin dose rato tho culculutoJ roan liver con to. , ime J/`;S 71 Iwa <jnly j/l3 tbo ioixlolty of Jirt', but ecu?..', bo none to. `e ot lor `0003 booouoo it op ocre 'o have ti tioi'j yradm-t norfcali.ty oivrvo than 1>P7* All birdr, oyiny fron PCD !ir/ er.ltu'jol Kidneys an' before loath r.o. o t'dcpi.vt 1 opyuront loy porulyclo or*body ml ui-c tron'olA y;. It m c :;eJ"'c tlu-.t J'C' \r.~. iuili:/ily to have cru.o * . Uoaproa*. .'otlvA. to:city in -..Alt' nvv ,o* bii'i; in rritatn, but could bo c coi ,po '.c. it cnur.'i of the prccont broot'd i.j f; iV. j'opori,'". in sovornl, opooio3* HONS 212493 f/ -- o ro:,YC:!W.,i;i-;' n n:-.r.:m. irnrbir::) An part of ft ro it'ii; rsurvey for pooiietOo rji'lduot> in Inportcd foot1/-inf vo mconlly extract'd rt.v^Ar.'O of fp'nuivl C"Vt trjf.ru C.oo of tlnro ii'vlo'f. rt>on rmaly.'i'wi by 4;.o-ii';uVl r.brf -'O'.^iyt ;\v''..:'.vl to coninin ;x>3./c'.tt o;i b.'tjh-myln of ibo iV' chloi'm nl\Vco ?y div.'Ot cvvm\V.ion of t'/j r. cj conij*nod About 10 rn 'fbo r1'm;>lr,* v n {-.Ho cnly coo or n ^v-vp if? f'.X'i tl'o C".*vj nbipvrit 0S nulo to co.iir.in tborto oojitp'itiimta rr.d Jnv'OtJ^'V i-'tvo'.vJcO. tv::it tlit:; cro r iC':o<l in u Jiffo fit rvwios tos truin'or L to t' Jnbosnloiv* Tbo r;'n;Ao J'.C boon ncolvc't jt.'C^ot*. in dJn at Jin (It;'"'!,:;* ),v.D'(UorcJ C'J.Obonri Orv.'.i, h foi.lbor.' ru;>r>ly of cnpty irJim mnn obtilu-.l r .* of thvo \.'v) ci wvl extent to j viih n-hcirmno c.nil nuloJLvtod to /y.iO'A'r.iO Ciij.o.'u tosTor-lito r.ntlyr.lm Uni onlr.ct frou tho c'r.'.n cont^i-'d ;.n }<\rnl?< -.1 florin0 of yooifi to the orVv.'ci of Cvj}ir.t mtn, tho cono:ubrftV.on i*i tbo ('' voa ootJ'oittd nt r>D p;>:i Tl3 0 rionndo Irivo I'f v >Auoo cot'i*ony'*n',ir;1 l:< ; - Cliloi'iii'itrJ bi;!r.iyl'; on Inir.-loyer lima tyretr try conTjv"'.1 ;.:1 pjLVaor.co of c'ilO''Jji.'.io M -.'r.njy'ln in tfco fijr.net* ibo pnrticP.T jv'to of 1' io cur'.1'fitly firvUu,; a 3nvuo nitbor of itr;>:<> ax.i;; '.:hioU i t?io p.'cV'ji');; c fpty f; ii.i',.1n c ft Port f fed in thin 1 .tor-.ilory for toxJcolojic 0 fy.Vy; Atutlyoio b '.'j nbo nt tbit 00 ;'jT.yoMiouJjv.V .1 b,' 1', i iyl n iron doir^t'Mr i'\ V, ,1 fljnilo- boforo .wilynio* "Jnoo c 1' ibo.:t.m rot Uith** rto b*m viiy^ri <1 c cvioin ; cnt*win itio.n cxvot tn*i rbonln Vo t lun to rrr.nvo tli'.t foc<*. f.\ vilrtlifo fl if.ploa .jub/ilttn'l to l1') f-to'.'jj for noitno tciniyo.'r. nr,3 p;.1: I \, or.nov.i bM: .ill not i>n -l'.'.-a Jvi.vt i 'riuu.j:- V> t`m t! ' lo'id lo ptionro'n flnt'Jnyo* 1 7hon;:.io'i ]'.tllf i',,0* uvt i', :t (`out* lb?J in tbo j.. r.nfnot >tf-n CoitrA I. nvolory Jlool; Ittoo i'votb Toino/th ftwruJ ton fucroy ' S -'U'v VJ 1 ? T[ VI (`Li ' S NONS 212494 Levels of organochlorine pesticides and polychlorinated biphenyls in nwgarine, vegetable oils, and some foods of animal origin on the Swedish market in J967--1969 by Citturl V/clloo, Kottht Svrf/i At it ,tiV git th'.L'iito:; In 1967 1967 ihc lesots of ors,.inOst>loi<nc peMicides and polychloiintied biphenyls (l*C*Vt strte determined m about 1 400 samples of marfanne, vejenblf ods. and foods of animal ongm Jji telannn lo ih, accept it'lc daily intaV.cs of the cl.lonr. <,cd pesticides and, die ni.ijjr.nudc of con lunipt on, hijjl. IcvcIj of ortfsnochlorine pesticides wn; found in one sample of erj >c Ik. some umples of SaL'i food prepated from poulny, sevctal samples of ft<h and all the samples of human nnifc lbs rest of lire samples fwd lost or modsr.ite lesels About 90 per cent of the food lantplos ti.testiyjird contained tt nsost 01 mjj/kf of the DPI Jioup 1DD7 + DDF, ~ DDD,. lindane. oEVIIC, and PCB. and at most 001 nis/kg of d'cldrin the pesticide levels found are tela led, beinfes 10 acceptable daily intjV.es, also to tempoury lol.-raneci and teiirporar) prac.ieat residue limits The practice of e\|ircssmg loleranrcs and prastieal residue limits for clilormned psesticides in meat and fish as mj/ky; far t; erwcucd 10 MQNS 212495 5 ftf 7 Cfikrociwtatrini iv ett mint exrtaki tv hUniLo'i exiuhcrat (tin tJuminiumou'diunnlikupljtta (Fig 1) Cm chrometovem of it detned-up et.'rj't of poultry extracted front an aluminium oxide thm- tayer plate (Fit 1). A Entrtkt filn too 7 mrd kloipcfticictrr mom D1)F. (1 n BHC. 2 lindtn, ) hepuklorepoiid (intern tttndaid). 4 etieldrin,J p.p'-DDT) A Extract of tone I Orianocblorme pesticide; except DDE B Exrnkt frln ton II. (ore o\idlion (6 PCR ocK 7 jij/.DDF) B Extract of tone tj, before oxidation C Fttrtkt (rln ton II. ejler oxidation (6 PCBj C Extract of tone U, after oxidation n MONS 212496 Tabetl t. Klorpevtldd- 'fcti PC3-tnlt I matfett, mg/Icg fett Oywlftmt prtticitU mi PCD Itrth tit //;, mj/it); fmt Vara Fttti Antal Antal prover mcd klorpcaticid- och PCB-halte; inom *u*a interva'I aruljr- Nmttbtr fmmf/tt with trgmoc/tfermt ptrtitiit md PCD !rtth withm ttrtmt rntfff icrrulc -----------------1 -- -- ------------------------- prortf Interval! for Intervall frir Numbtrtf DOT, DOE, dirlorm jvap/r/ DOI), o+BI IC Rjtr^r tfljttd nch lindan Ratt^t rre^/kg fctt fctt DDT DDE DDD n-BHC Lindan Dieldrin Interval! for fC3 mS/'ig fett PCB Smdr Slnffrr 154 Mafgnitn Afar^ertm 115 Vc^ctatyIn olfor VefrtMt mlr bipn^iotfor wW 0th 49 rrajsofja, olivolja tmn mft thm ml 84 C -0.03 123 37 154 o.(m --o.to 23 117 0,11-0.30 3 0.3!-t.0 1.1 -2.5 >2.5 85 63 6 0-0.03 115 115 115 115 0,04-0,10 0.11-0.30 o.v,_;.o 1.1 -2.5 >2,5 0-0,03 48 48 48 48 0.04-0.10 0.11-0,30 0.31 -1,0 1.1 -2,5 >2,5 0-0.03 84 84 84 84 0,04-0.10 0,11-0,30 0.31-1.0 1,1 -2.5 >2,S 148 0-0.010 129 5 0,011-0.02 19 1 0,03-0.05 5 0,06-0,10 1 0,11-0,20 >0,20 115 0-0.010 115 0,011-0,02 0.03-0.05 0.96--9,t0 0.11 -0.20 >0.20 48 0-0,010 48 0,011 -0.02 0.03-0,05 0,06-0,10 0,11--0,20 >0,20 84 0-0,010 84 0,011-0,02 0,03-0,05 0,06 -- 5,10 0,11-0,20 >0,20 0-0.1 0.2-0.5 0,6-1.0 U -3.0 3,1-7,9 >7.0 0-0,1 0,2-0.5 0,6-1,0 1.1 -3.0 3.1 -7,0 >7.0 154 115 0-0.1 9.2-0.5 0,6-1,0 I.!-3.0 3.1 -7.0 >7.0 0- 0.1 0.2--0,5 0.6-1.0 1.1-3.0 3,1 --7,0 >7,0 48 84 H0*S 212497 4 so}*', 'infrw-, safflor- och scs&mo!fs *ybfmt ftmfltwtr. ltnttt4> ttffltwtr. amtf Itfurmt *f!i 65 9-0.03 58 65 65 5? 0,04-0,10 6 4 0,11-0,30 1 031--1.0 1 1 1.1-2.5 >2,5 62 0-0.010 64 2 0,011-0,02 1 0.03-0,05 * 0,06--0.10 0,11-0,20 >0,20 0-0.1 0.2-0.5 0.6-1,0 1.1 -3.0 3,1 --7,0 >7.0 Tabetl 2. Xtotpcadfid* ch PCS-halter i mjOIV, mg/kg fctt Orgmatitarim ptt/itiJt and PCD ftarit in milt, mg/Jbgfat Vnra Pmi Antal Antal proTcr med klorpcsticid- och PCB-halter mom risss rotcrrmll arwlr- Nttmbtr iftarnpitt mth orfjsimhtvnm ptrftctdt *r*d PCD frith wrtbt* (trtarm nwjf/ scrule ^ron*t Ntuttffrrrf inmfilet 1n1`t>mteIr)\TI(,Si-i1lRl3f0oU3rC, Intersil for d*eldrm R*rift Xh IlfKi'Vl tng/kg fett ftanfjt npj/kq'fcit DDT DDE DDD -BHC Ltrutin Dseldrsn !ntrrill for PCB Ran^t ing/i.g fctt PCB Kotnj&lfc Cam't milk ModcnmjStJc Mtrman milk n 0001>,,,-311201-1,,5--12:00,.5o.30 11 9 11 11 2 22 000,3-1011,--1001.,300 8 22 22 1>,12.-523 14 18 4 11 0000>,,,-00010361^.--0-00200.,,201050 10 1 22 0000,,.1-001360---,-90002..,021500 A1i 5 4 2 >030 0031>..,-1617--0-.01,537..,000 0301,..--116--0-1,537.,,000 >7.0 11 11 11 M0"S 212495 JJ TibtH 3. SlipatlcU> och PC3-Im!ter ] fetter Mb anlrnele thmedtl, mgfkg limnedel OrjywbUrmr prrtrriJt mit PCD Inti> At /!r/r mit fmit */ mrinml migjn, mgfkg fmi V'ira /w Antal Antal p*wtt mcJ Vlorpcsticitl- och PCB-hnl'er inn vis interval? iwkvIt- Number / mmpter with orjpiwbfenm prsisctct *m PCB M rntf/rn errtmin rvmfrr 9CtVlC pfover Interval* for Interval! for Number if DOT, DDK, tiicltinn ttmpht 1) OD, Bf 1C memijftd oth ItmJan hvs- Rtmft mctril IStv nnslcl DDT DDE DDD o-BHC Lindait DWdrin Intrmll for !>CB rrv-Jk^ lirvmakl PCB KyeMbw(HK) Cbffktn (mm/) 43 Broiler (kdtt) Brrihr (mm/) 50 H8n* (IgBtt) Hrv (mm/) 50 Await (vaniigen 4 igg/pror) 185 0-0.03 43 43 43 43 0.0*-0.10 0,11-0,30 0.31 -1.00 1,01-2,5 >2.5 0-0.03 50 50 50 50 0,(K-0,10 0,11-0,30 0.31-1.00 1.01-2.5 >2.5 0-0,03 46 36 50 50 0.04-0,10 4 13 0,11-0.30 1 0.31-1.00 1,01--2,5 > 7*> 0-0,03 145 72 184 182 0,04-0,10 31 104 2 0,11-0.30 0.31-1.00 7i 7 1 1 1 1,01-2,5 1 >2,5 i 43 0-0,010 43 0,011-0,020 0,021-0,05 0,06-0,10 U, 11 -- 0,20 >0,20 50 0-0.010 ;o 0,011-0,020 0,021-0.05 0,06-- 0.10 o.i! -0.20 >0,20 48 0-0,010 46 2 0.011-0.020 2 0,021-0,05 2 0,06-0,10 0.11-0,20 >0,20 164 0-0.010 174 17 0,011-0.020 8 3 0,021-0.05 1 1 0.06-0,10 2 0,11-0,20 >0,20 0-0.1 0.2-C.5 0,6 -1.0 1.1 -3.0 3,1-7,0 >7.0 0-0.1 0,2-0,5 0.6- 1,0 '..1 -3.0 3,1-7,0 >7,0 0-0.1 <\2- L\5 0,6-1,0 1.1-3.0 3.1-7,0 >7fl 0-0,1 0,2--0,5 0,6-1,0 1.1-3,0 3.1-7.0 >7.0 43 50 50 183 2 HONS 212499 Aggrft* (vanligen 6ifff/pcov) /Egg wbttr 28 HcHqj; (54 top 58 acc/pttjv) Vtmh tff. 2 $*mkdtt, ImTud- *9icii|^n flflik Park. 107 Ijmmkdtt Lamb (mat) 26 LamanTett Lamb fat 10 Osk&tt litrf 34 ' 0-0,03 0,04-0.10 0,11-0^0 041-1.00 1,01 -2,5 >2.5 0-0.03 9,04-0.10 0,11-040 0,31-1,00 1.01--2,5 >2.5 0-0,03 0,04-0.10 0,11-040 041-1,00 1.01-24 >2,5 0-0.03 0.04-0.10 0,11-0,30 0.31-1.00 1,01-24 >2,5 0-0.03 0.04-0,10 0,11-040 0,31-1.00 1,01-2,5 >2,5 0-0,03 0.04-0,10 0,11-9.30 0,31-1.00 1,01 -2,5 >2,5 28 28 28 93 105 106 It 2 31 26 26 26 10 to 33 M 1 28 0-0.010 28 0.011-0.020 0.021-0.05 0.16-0,10 0.11-0.20 >0,20 0--0,010 2 0.011-0,020 0.021 -0.05 0,06-0,10 0.11-0.20 >0,20 106 0-0,010 107 l 0.011-0,020 0,021-0,05 0,06-0,10 0,11-0.20 >0,20 26 0-0,010 26 0.911-0.020 0.071-9.95 0,06-0,10 9,11-0,20 >0.20 10 0-0,010 10 0.011-0.920 0,02!-0,05 0.06-0,10 0,11-0.20 >0,20 0-0,010 34 <\0l1 -0,020 0.021 -0,05 0.W-0.I9 <U 1-0,20 >0,20 0-0.t 0.2-04 0.6-1,0 1.1-3.0 3,1 -7,0 >7,0 28 0-0.1 0.2--0,5 0.6-1,0 1,'. --3,0 3.1-7,0 >7,0 0-0.1 0.2-0,5 0,6-1,0 U-3,0 3,1 -7,0 >7.0 106 1 0-0.1 0.2 0.5 0,6-1,0 1.1-3,0 3.1-7,0 >7,0 26 0-0,1 0,2--0,5 0/--1,0 1.1-3,0 3.1-7.0 >7,0 0-0,1 0,2 -0,5 0,6-1,9 1.1-3,0 3.1-7,0 >7.0 10 HONS 212500 TaMt 3 (farts) Vara Paai Art*.' Ant*', prorer mod Vlorpeatkid- och PCB-liiItn tnom ;* interval! imljr- Number afsamples with arfanoAhriKtpe<iieide and PCI! Intis mtldm imam nstjftr acnde ----------------------------------------------------------------------------------- proper l^fcryaF. fdr ImerrjL'f Air N?trrrWr*f SOT, 0!>l*. diciiErtn mmpftr 2Dl)t **Ht lC och rru.'k* Rm rrcilci lfv$> mcilc DDT DDE DDD o-BHC Lindan D-cldnn Inverr*fl for VCB H/trtft tIit*- TtlCt'cI PC3 Hale, s&tmtten frrtb sratar fnrh*li > 4 % 2+n* 0-0,03 2 fat ptmatagt > 4% 0,04-0,10 0,11-0,30 1 2 2 0.31-1,00 1 ',01-2,5 >2.5 fctthalt S 87+13* 0-0,03 76 61 86 85 fat fmtnlagt S 4 % 0,04-0,10 10 23 1 2 0,11-0,30 1 3 0,31-1,00 - 1.01-2.5 >2,5 Fak, nftTtten fiM, marine frtthnlt > 4 % 34 + 5* 0-0,03 5 6 10 20 fat pertentaft > 4 % 0,04-0,10 4 8 13 5 0,11-0JO 12 14 10 0,31-1.00 12 6 1 1,01 -2,5 4 >2,5 fctthftlt 4 % 65 A' frrtmtap 4% 0-0,03 43 40 57 64 0,04-0.10 14 1? 8 1 0.11-0,30 8 6 0,31-1,00 1,01 --2,5 >2,5 2 0-0,010 0,011-0,020 1 0,021 -0.05 0,06-0,10 1 0,11-0,20 >0.20 11* 0-0,1 0J-0J 0,6-1,0 1.1-3.0 3,1 -7,0 >7,0 1 t 86 0-0,010 71 0-0,1 86 1 0,011 -0,020 0,2 -0,5 0,021 -0,0? 2 0,6-1,0 1 0,06 -0,10 !, 1-5,0 O.'l -0.20 3,1 -7,0 >0,20 14+13* > 7,0 34 0-0,010 10 o.or.-0,020 4 0.021 -0.05 0,06-0,10 0,11--0,20 >0.20 19 1 5* 65 0-0,010 49 O.Cll-0,020 1 0.021 -0.05 4 0,06-0,10 2 0.11-0.20 1 >0,20 8 0-0,1 0,2-0,5 0,6-1,0 I, I-3,0 3,1 -7,0 >7,0 0-0,1 0,2-0,5 0,6-1,0 1,1-3,0 3,1 -7,0 >7,0 13 18 2 58 $ 1 HONS 212501 oljor l/n>fMr tilt (100 % fett, *e tab 1) Vw^aon Af-irgmrim 115 " 0-0,03 115 115 115 115 0.04-0,10 0.11-0,30 0.31-1,00 1.01-2,5 >2,5 Smflf Bntttr KonjfilV Ctw'i milk Mcdeninfdlk //*M milk 154 0 - 0,03 134 47 154 101 0,04-0,10 17 107 4? 0,11-0,30 1 5 0,31-1.00 1.01-2.5 >w 11 0-0,03 11 11 11 ii '*,04-0,10 0,11-0,30 0.31-1,00 1.01 -2,5 >2,5 22 0-0,03 11 1 22 22 0,04 -0,10 11 19 0,11 -0,30 2 0,31 -1,00 1,01-2.5 >2,5 BabytilUfy* fnpp/tmtns Barnmnt Btby fmi 13 0-0.03. 13 13 13 13 0,04-0,10 0.11-0,30 0.31-1,00 1,01-2.5 >7,5 171 0-0,03 171 145 157 171 0,04-0 10 14 7 0,11-0.30 74 0.31-1,00 1.01 --2,5 53 ________ >2.5 1 endnst dieldrio bar bettSmtl 1 thtUfnt m tltftrmmrJ 115 0-0,010 115 0,91:-0,020 C.C21 --0.05 C.06-0,10 0,1!-0,20 >0,20 151 0-0,0*0 135 3 o,o:;-0,020 15 0.021 -0,05 4 0,05-0,10 0,11-0,20 >0,20 11 o-o.oto 11 0,011 -0.020 0,021-0,05 0,06-0.10 0,11 -0,20 >0,20 22 0-0,010 22 0,011 - 0,03) 0,021 --0,05 0,06-0.10 0,11 -0,20 >0,20 13 0-0,010 13 0.9! 1-0,020 0.921-0,05 0,06-0,10 0,11-0,20 >0,2C 171 0- 0,010 171 0.01*.-0,020 0,921 -0.05 0,06-0,10 0,11-0,20 >0.20 0-0,1 0.2-0.5 0,6-1,0 1.1-3,0 3, '-7,0 >7,0 c-o,: 0,2-0.5 0,6-1,0 1.1 -3,0 3,1- 7 0 >7,0 0-0,1 0,7 -0,5 0,6-1,0 1,1 ^3,0 3,1 -- 7,0 >7,0 0-0,1 0,2 -0,5 0 6- ',0 -3,0 3,1 --7,0 >7,0 0-0,1 0,2 -0,5 0,6 -1,0 1,1 -3,0 3,1-7,0 >7.0 0-0,1 0,2-0,5 0,6-1,0 1,1 -3.0 3,1-7,0 >7,0 115 154 i: 22 13 171 HONS 212502 M TabeU 4. Klorpeatlcid- ocS PCB*Mtcr (<tA<C few) 1 [lijmedd m*< ea DOT-, DDE-, DDD-, Iindn- ellei o-BlIC-halt #tr 0,10 mj/kg fctt lter ta d:e!drnh*l( #wr 0,02 mg/kg ftK ' Orfrnnttl'ltnm pntiair mi 1*CB ftrrfr m f--Jr with a DDT, DDE, DDD, fmimr, #r tt-BHC Irrrf tbmr 0. TO atmr0.02 fmi ftft #r a iiieiiiffri irttf Vtn Fmd Datum Daft Tetthalt % p,p'-DDT o,p'-DDT p,p'-DDE p,p'.DDD o-3HC Z7" % Lindai DfeWrift PCS ScMmvIp Stsamt m! 06.05 69 Sojaoljt Srjbimw if 29.05.69 Sm6r tirtilrr 23.05.66 02.06.66 11.07 67 24.07.67 25 08 67 15 12 67 15 1267 17 99 68 17.09.68 06 02 69 06 02.69 06.02.69 20.02.69 KomjOlli CVjtV milk 28 06 67 17.07,67 Modcf?rtij^llt 1 Httmrnn mt/Jb 2 3 4 13.04.67 26 04.67 16.05 67 18.05 67 100 82 3.0 2,9 3,0 3.1 3.3 0,28 0.05 0,03 0,03 o.n 0,04 0.13 0,08 O.*0 0,11 0,09 0,02 0,03 0.03 0,03 . 0,035 1.4 1.7 1.3 1.1 apif 0,02 0.C6 0,06 0.04 0.04 0,05 0,C5 0,04 0,02 0,05 0.05 0,03 0.16 0.15 1,4 1,4 1.9 2,0 <0.01 <0,01 <0.01 <0,01 <0.01 0,01 0,73 0,14 0,04 0,15 o.n 0,06 0,14 0.17 0,16 0.17 0,04 0.05 0.05 0,04 0,06 0,04 0,65 0,02 0,05 0,017 0,07 0.04 0.01 0.92 0.02 0.03 0.03 0.01 0,12 0,05 0,03 0,001 0,01 0,036 0,039 0.C29 0,91 0.009 0,011 0.999 0,009 0.03 0.02 0.03 0,06 0.035 0,07 Spilt sprit *pir 0,03 0,02 0,05 0.01 0,02 0,02 0 04 0.03 0,04 0,04 0.06 0.04 aplr 0,7 0.3 C.3 0.6 HONS 212503 s 22.05.67 6 30.05.67 7 01.06.67 8 05.06.67 9 210667 10 26.06.67 11 26 07 67 12 27.07.67 15 27.07.67 14 0i.0S67 15l 01.03.67 16' 2.V.0 68 17 20.11 *8 18- 19 01.69 19 1* 08.69 20 19 08 69 21 07.TO69 22 07 10 69 * (Win en pcmm 2.9 3.2 3.3 2.9 3.9 2.9 3.0 3.3 3.8 3,2 4,0 2,0 2,9 4.4 3.0 2,7 3.3 3.0 U 1.0 1.0 1.0 0.9 U 2,0 1.6 1.0 1.3 2.0 1,0 0,8 1.4 1.1 1.3 1.1 ` O.S 2.1 2.1 1.7 1.8 1,6 2J 3,1 2.7 2,0 2,5 3,7 2,0 1,6 2.9 1,8 2,1 2.0 1.1 spAr tplr spy-'ir *fvr 0.02 0,01 spAt spcir Sp-*r pif 0,02 0.02 *pir p&r *pir *p.lr 0,05 0,07 0,03 0,13 0,03 0,05 0,05 A 0.07 0,07 0,11 spar 0.7 0,6 0.3 0.3 0,3 0.7 0,7 0J 0.3 0.3 0.5 0.5 C,5 0,7 0,7 0.7 0,6 0.7 NJ HONS 212504 Jg T*b*H 5. Klwpeptlcld- och PCB-Mter (mj/Iqj) i Uvsmedel son hm on DUT-* DDE-, DDD-, llndsn- eller a-BHG-Iult Poor 0,10 mp/lcg livsmcdcl ellet m dieldrinhal; 8srr 0,020 mg/kg Hrimedel och *i tidigan rodosisats tsbel! 4 Orfptmfb/mrtne ptifieide mmt Pdi itwrh rw fmdt *1tirh Ame * DDT. DDFz, DDD, tfuftnr. tr u-RFfC /W *bt* 0.030 MgfkzfotJ*nJ trr mt prm'mttlj nptrtrd m 7tbit 4 Q TO fftd *r * dicfdrin Ifwrl Van Fmi Dutum Daft p.p'-DDT o.p'-ZJDT p,p'-DDE p.p'-DDD o-B! 1C Limlrn Dreldrin PCS 1 l<kl (Mtt) Hm fmttmi) Aiy*ult Frjy^t 04 11 68 04.11.68 0.02 0.04 0.10 0,!2 0,006 0.905 0.006 0.905 0.024 0.924 0.08 0,03 30.04.68 0.11 0.07 ?p.r 30.04.68 0.13 0,98 spV 15 05.68 0,03 0.95 0.002 0.009 0.03 0,05 15.0168 0.08 0.11 n.07 15.05.68 0.05 0,11 0,02 22.05.68 0.03 0.06 0,007 Jp6r 0.011 0.03 0.06 24.05.68 5,6 0,31 1.56 0.15 0,005 0,909 0,92 0,01 24.05 68 *par 0.95 spfir 0.037 9.03 27.05 68 o.ll 0.15 -.p.*r 0.03 20 05 68 spar 0.04 0,28 '0 06 68 0,20 0,11 0,016 10 06 68 0.09 0,13 9,012 0.95 10 06 63 0.08 0.11 0.002 0,009 0.09 10 06 68 10.06 63 0.07 0.11 0,02 0.002 0.91 0.12 0,016 0.1 1 '.06 68 0,05 0,37 17 06 68 17 06 68 17 0663 24.07 63 25 07 68 0.30 0.15 0.60 0,25 0,01 0,1 C 0.08 0/2 0,09 0,24 0.10 0.'2 0.02 0.04 0.05 spir 0.03 0.05 0.03 MONS 212505 Sttfflbk Ptrk Btawit B*ff* hOnt btf 13.11.67 2Z.0t.43 26.01.68 06.02.68 07.02-68 12.OZ.68 0,1* 0,15 0.72 0,12 0,72 0,20 01.03.63 Cl .03,63 01.03.68 tt.0*.68 '1.04.68 22.04 68 22.04.6S 30 07.68 30.0768 30.07.6S 02.08.63 02 0S.< 0,0*5 o,os 0.12 0.03 0,29 0.17 0.06 ipit 0,02 *p4r ipilr 030 0,0:3 Jpir ipAr spur 0.11 0.33 0^45 0.90 0.13 w 0,15 0.12 0,27 0.11 0.60 0,12 0,055 0.22 0,26 0.75 0,10 0.69 0,12 0,09 0.13 0.07 0,60 0.05 0,010 0,003 0.006 0.903 HONS 212506 so Tmbcll 6. KlwptufcM* och PCB-tnlter 1 mpderwnjOBt. tn~/kg m)BUt Onpmtbhrim frttiait mtJ PCH Irrtb m Imwi mt/k, milk Pnw n Datum SampU N* D*tt Fetthalt % pap'*DDT o.p'-DDT p.n'-DDE p.p'-DDD o-BHC /v% 1 13.04.S7 2 26.04.67 3 16.05.67 4 1*05.67 5 22.05.67 6 30.05.67 7 0106.67. 8 05.06.67 9 21.06.67 10 2606.67 tt 26 07.67 12 27.07.67 13 27.07.67 14 01.0*67 ts> 01.08.67 16* 28.10.68 17 20.11.68 18' 19 03.69 19 18.08 69 20 19 08,69 21 07.10.69 22 07.10.69 1 frikn en ptnm 2.9 3.0 3.1 3.3 2.9 3.2 3.3 2.9 3.9 2.9 3.0 3.3 3.8 3,2 4,0 2.0 2.9 4.4 3.0 2.7 3,3 3,0 0.041 0.050 0.041 0.036 0,034 0,033 0,032 0.028 0.035 0.039 0.061 0,053 0,040 0.043 0,079 0.020 0,024 0.064 0.033 0,035 0.035 0,023 0.040 0.043 0.058 0.067 0,062 0.066 0.055 0.051 0.061 0.067 0.093 0,089 0,075 0,081 0.15 0,041 0.047 0.13 0,055 0.058 0.067 0.033 0,0005 >pir *p-1r jpdr 0.001 0,0902 0.002 Lindin ipJi ipir spur ipflr 0,0005 o.cot Dkklrin PCS 0,002 ipit jp-ir Spaf *r',r soar iy*ir ,, 0.002 ijrif 0,002 0.001 0.005 0,001 0.002 0.001 0.O0C5 O.OC3 9,002 0,003 splf 0.02 0.01 0.01 0.02 0,02 0.02 0.01 0.01 0.91 0.02 0.02 c.o; 0,01 o.c: 0.02 0,01 0.01 0,03 0,02 0,02 0,02 0,02 MONS 212507