Document yr2Mz0nOkgaqboBGp675mG9pd

AR226-2767 FOR DU PONT USE ONLY E. I, du Pone de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road I Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 567-81 Material Tested Octanoic acid, pentadecafluoro-, ammonium salt* Haskell No. 12,037 Other Codes pfl- 14,033 14,034 Study Initiated/Completed 4/6/81 - 5/14/81 .Material Submitted by R&D Department Haskell Laboratory ORAL LD50 TEST IN RATS Purpose? To^detennine the effects of] if any, on the LD50 vs [ie o and n rats. Frocedare: Thirty young adult male CrltCD rats were, treated by single intraperitoneal 1'jectlons with aqueous solutions o f f l P H B H M M H M N H B j a t 80 mg/kg/day for 3 days. One day ^ a f t e r t h e final treatment, the 30 rats, 10 rats per group, were a d m l n l s t e r e d p B B g ) | b y intragastric intubation as a suspension in corn oil in single doses to determine the LD50 value. The surviving rats were weighed and observed during a 14-day recovery period and then sacrificed. The LD50 value was calculated from the mortality data using the method of D. J. Finney.** ' Another group of 30 male rats was treated by singlelntraperitoneal injection with aqueous solutions o f [ j ^ H H I H i [ | ^ H f l t t H i i at: 50 mg/kg. One hour after t r e a t m e n t f j B H B y ? & s admfhistered, u s i n ^ t h ^ s a m e procedure as above, to determine rhe LD50 value. An additional g r o u p o f 30 male rats served as controls and was administered only t h e p B M f t J b y intragastric intubation to determine the LD50 value. The same procedure was used. - 1- Company Sanitized. Does no! contain TSCACEtf r**. O H 1 td Results: Control t] *Dose (mg/kg) Average Body Weight (g) Suspension (Z) Average Dose (mL) Mortality Ratio LD50*** 650 500 400 267 276 262 4 4.32 8/10 478 mg/kg 3 4.59 7/10 3 3.49 2/10 Clinical Signs: Wet and/or stained perineal area and weight loss at all levels tested. Stained face and weakness at 650 and 500 a g /k g .mg/kg. Chromodacryorrhea at 500 All deaths occurred within 6 days after dosing. Dose (mg/kg) Average Body Weight (g) Suspension Average (%) Dose (mL) Mortality Ratio LD50**** 650 500 400 259 260 261 4 4.21 19/20tt 547 mg/kg 3 4.33 4/20 3 3.48 0/20 Clinical Signs; . Vet and stained perineal area, stained face, diarrhea and weight loss at all levels tested. Weakness at 650 and 500 mg/kg and lethargy at 650 mg/kg. All deaths occurred within 4 days after dosing. Dose (mg/kg) 650 500 400 Average Body Weight (g) 269 256 261 Suspension Average (Z) Dose (mL) 4 4.37 3 4.27 3 3.48 Mortality Ratio 8/10 5/10 1/10 LD50t 520 mg/kg Clinical Signs: Stained face, wet and stained perineal area, weight loss and weakness at all levels tested. Diarrhea at 650 and 500 mg/kg. Tremors and chromodacryorrhea at 400 mg/kg and Jacrlmation at 650 mg/kg. All deaths occurred within 5 days after dosing. - 2- 'wipanv SanHfted. retain cm Summary: There were no significant differences in the LD50 valui i d a g leither.whep tested alone or following pre-treatment with either The LD50 f o r H H M i ! ^ 4 7 8 Eolio Ls 520 mg/kg a n M j H f e p o l l o wwiinnga ^ |is 547 mg/kg"1>f body weight. 1 Clinical signs most frequently seen included: wet and stained perineal area, stained face, weakness, diarrhea and weight loss. All deaths occurred within 6 days after dosing. H-12,037 Synonyms : Purity: Contaminants : H-14,033 Synonym: ^jj CAS Registry N o . :l| Purity: H-14,034 Synonyms: CAS Registry No.: Purity: ** Finney, D. J., Probit Analysis, 3rd Ed., 1971, Cambridge University Press. *** 952 Confidence Limits: Lower: Upper: Slope: 361 mg/kg 571 mg/kg 7.9 **** 952 Confidence Limits: Lower: Upper: Slope: 517 mg/kg 582 mg/kg 22.1 - 3- noScertain TSCACBI Sanitized- Des Compaq t 952_Confl^jenceJLimlt: Lover: 450 mg/kg Upper: 618 mg/kg Slope: 9.8 tr this study was repeated. Rats were based on all 60 rats. Report by: ] / [ *.1w v V John E. Henry/ Technician Reviewed by: S t "' s fj /0^.7f7LAou<is Dashlell Study Director Approved by: C *__________J_T_.______ ''M V _____ J b .Gerald L. Kennedy, ! C h i e f A c u t e Investigations Section JEH:jrg:MP:1.12 Date Issued: Septen her 30, 1981 Report No. 567-81 4- Compar Sanitized. Does noc<**contain SC CS B