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BACK TO MAIN .. Study T i t l e A c u t e T o x i c i t y of L - & l J 9 2 t o t h e Daphnid, Daphnia magna D a t a Requirement: G u i d e l i n e Sturnber U.S. EPA-TSCA, Guide:Line 797.1300 Authors Timothy J. ward Jeanne P. Magazu R o b e r t L. B o e r i Study Ccnoploted J u l y 13, 15195 Sponsor 3 M Company Environmental LaDoratory 935 Bush AvEmIe Building 2-3509 St. P a u l , Minnesota 5 5 1 4 4 Testing Facility T.R. W i l b u r y L a b o r a t o r i e s , Inc. 40 w a k s Lane Marblehead, Hassachvsetts 01945 T.R. w i l b u r y S t u d y Number 840-TH Page 1 of 22 --- BACK TO MAIN I . GOOD LABORAUITORY PRACTICE COSWLXANCE S T A T W E " This study was conducted according to EPA cood Laboratory Practice Regulations (40 CFR 7 9 2 ) . with the following exception. The stability of the test substance was assumed but not verified at T.R. wilbury Laboratories. Devlations from the study protocol and protocol amendments are presented in section XI1 of this report. i study i~irector T.R. wilbury study Number 840-TH Page 2 of 22 BACK TO MAIN XI. QUALXTX A S S U W C E STAT- submitted by: T.R. wilbury Laboratories, Inc. 40 Doaks Lane Marblehead, Massachueetts certification: T.R. wilbury Laboratorien, roc. study number 840-TH, Acute Toxicity of L-13492 to the Daphnicl, Daphnia magna, was audited by the Quality ASSUTdnCe unit for compliance with the protocol, standard operating procedures, and applicable Good Laboratory Practices ( 1 J . S - EPA, 1993). The original raw data and a copy of the final report will be archived at T.R. Wilbury Laboratories, Inc. for at least 10 years. Quality assurance audits were performed .andthe findings reported to T.R. Wilbury Laboratories management and the study director on the following dates: Aspect Audited Protoco1 : In-lif e: Draft Report: Final Report: Audit Date 6/ 8/95 6/28/95 7 i 5/95 7/13/95 Reported to Study Director -- b / 9/95 6/2a/95 7 1 5/95 1/13/95 Reported to Hanagenent 6 / 9/95 7/13/95 7/13/95 7/13/95 Quality Assurance officer T.R. wilbury Study Number 840-TH Page 3 of 2 2 BACK TO MAIN SECTION: I. Good Laboratory Practice Compliance statement 11. Quality Assurance statement 111. Table of Contents IV. Index of Tables and Figures V. Summary VI. General Information VII. Introduction VIII. Methods and Materials Ix. Results X. References XI. signature Page XII. Protocol Amendments and Deviations Appendix A. Water Quality Data from Toxicity Test PAGE 2 3 4 5 6 7 e 0 13 17 la 19 20 T.R. wilbury study Number 040-TH Page 4 of 22 BACK TO MAIN IV. INDEX or TABLES AID FIOURCS Table 1 Table 2 Table 3 Table 4 Table A . l Figure 1 Chemical characterization of a representative sample of dilution water used for the toxicity test with daphnids, Daphnia magna, and L-13492 Test system summary for the toxicity test with L-13492 and daphnids, Daphnia magna Survival and sublethal effect data from the toxicity test with daphnids, Daphnia magna, and L-13492 Median lethal concentrations (LC5Os) and medi.an effective concentrations (ECSOs] from the toxicity test with daphnids, Daphnia magrla, and L-13492 Dissolved oxygen concentration, conductivity, pff, and temperature measured during the toxicity test with daphnids, Daphnia magna, and L-13492 Survival and sublethal effects of daphnids, Daphnia magna, exposed to L - 1 3 4 9 2 for 48 hours PAGE 9 11 14 16 21 15 T.R. wilbury Study Number 840-TH Page 5 of 2 2 BACK TO MAIN The scute toxicity of L-13492 to the daphnid, Daphnia magna, is described in this final report. The test was conducted for 3H company for 48 hours from June 27 to 29, 1995, at T.R. Wilbury Laboratories, Inc. in Marblehead, Massachusetts. It was conducted by Jeanne Magazu, Jacqueline Nevius, Peter Rowalski, Keven Stevens, Peter Nevius, Robert Boeri, and Timothy ward according to the protocol developed for T . R - wilbury Study Number 840-TH. L-13492 was supplied by the sponsor. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20.4 to 2 0 . 7 O C . The dilution water was filtered deionized water collected at Marblehead, Massachusetts and adjusted to a hardness of 164 mg/L ES CaCO,. Aeration was not required t o maintain dissoLved oxygen ccrcentrations above an acceptable level. Nominal concentrations of L-13492 were 0 (control). 13, 22. 36, 60, and 100 mg/L. Daphnids used in the test were less them 24 hours old at the start of the test. They were produced at T . R . Wi1.bury Laboratories and acclimated under test conditions for their entire life (cultures that produced test specimens were maintained a t test condit:.ons for more than 7 days). After 48 hours of exposure the c:ontrol daphr.i.ds had an average wet weight (blotted) of 0.36 mg. All dap!inids were in good condition at the beginning of the study. Exposure of daphnids to the test substance resulted in a 48 h o u r median lethal concentration (LCSO) of 77 mg/L L-13492, with a 95% confidence interval of 60 to I00 mg/L and a 48 hour median effective concentration (ECSO) of 34 mg/L L-13492, with a 95\ confidezce interval of 30 to 39 mg/L. The 48 hour no observed (effect concentration (NOEC) is 13 mg/L. T.R. Wilbury Study Number 840-Tfi Page 6 of 22 BACK TO MAIN V I . GENERAL IWCORIUTION Material Teated: sponsor : Test substance shipped From: Reported Purity: Study Initiation Date: Experinental Star: Date: Experimental Coepletion Date: Study Completion Date: Archive Location f o r the 9iological Rrd Data and a Copy of the Final Report: L- 13492 3H Company Environmental Laboratory 935 Bush Arenue Building 2.-3E-09 St. Paul, Minnesota 55.144 3M Company Environmental Laboratory - 935 Bush Avenue Building 2 -3E 09 St. Paul, rlinnesota 55144 1000 June 14, 1995 (protocol signed) June 27. 1995 June 29, 1395 July 13, 1 9 Y 5 T.R. wilbury Laboratories, Inc. Harblehead, Massachusetts T.R. wilbury Study Number 840-TH Page 7 of 2 2 .-- BACK TO MAIN VI I. IIITRODUCTION This study was sponsored by 3H Company, St Paul, Rlinnesota. The objective of the study was to determine the .24 and 48 hour median lethal concentrations (LC50s) and median effective concentrations (ECSOF) of the test substance to the daphnid, Daphnia magna,. a freshwater invertebrate, under static conditions. The test protocol was based on U.S. EPA methods (U.S. EPA, 1993). The report contains sections that descr.tbe the methods and materials employed in the study, and results of the investigacion. The report also contains an appendix that presents water quality data collected during the test. VIII. METEODS AND MATERIALS TEST SUBSTANCE: T;.e sample of L-13492 (T.R. Hilbury Laboratories Sample Rumber 5 0 2 ) was delivered to T.R. Wilbury Laboratories o n .rune 14. 1995 in a cardboard box at ambient temperature. It was contained in a 125 ml p.lastic bottle. The label attached to the bottle included the following information: 'L13492, Hazardous Components/special Precautions: Isopropyl Alcohol. Originator: S.J. Martin + S.A. Beach, 53-412-02 4 2-3E-09, 8 - 5 7 7 4 . 8 - 7 0 6 5 , 5/4/95, 6/1/95, N2332-1'. L-13492 (a thin, clear liquid) was supplied by 3.y Company. st Paul. Minnesota. Prior to use the test substance vas stored in the dark at room temperature. Test substance stock solutions sere formulated in dilution water without the use of a solvent. The test. substance viis assumed t o have a purity of 1000 active ingredient and to be stable under storage and testing conditions. Unused test substance will be retlirngd to the sponsor. DILUTION HATER: Water used for acclimation of test organisms and for all toxicity testing was deionized water collected at T.R. Wilbury Laboratories in Marblehead, Massachusetts. Water was adjusted to a hardness of 1601 8 0 mg/L as caco, and stored in 500-gallon polyethylene tanks where it was aerated and continuously passed through a par':icle filter, ultraviolet sterilizer, and activated carbon. Results 01' chemical ae.alyeis of a representative sample of water are presented in Table 1 . TEST ORGANISM: Juvenile daphnids, Daphnia magna, employed as test organisms were less than 24 hours old at the start of the test. They were f r o m a single source and were identified using an appropriate taxonomic key. Test specimens were produced by adult daphnids that were maintained under test conditions at the T.R. wilbury facility for m o r a than 7 days. T.R. wilbury Study Number 840-TH Page 8 of 22 BACK TO MAIN Table 1. chemical characterization of a representative sample of dilution water used for tho tozicity toat with daphnids, Daphnia maqna. and L-13492. Paramet er Unit of Measurement Detect ion Limit Measured value PH Hardness Alkalinity Residual Chlorine Total organic carbon Total Phosphorus !a itrate Chloride Metals Arsenic Cadmium Copper Fluoride Boron Alum1 nu3 chromi um cobalt Iron Lead Mercury Nickel silver zinc organochlorine Pesticides plus Toxaphene Organophosphorus Pesticides PCBS pH units mg/L as CaCO, mg/L as C a m , mg/L mg/L mg/L mg/L as N mg/L mg/L mg/L mg/L mq/L ng/L ng/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L pg/L Pg/L Pg/L -- ---- 0.01 1 0.03 0.05 1 0.01 0.0002 0.005 0.1 0.5 0.1 0.01 0.03 0.03 0.005 0.0003 0.03 0.02 0.02 0.5 2 0.52 0.5 8.5 164 131 ND ND 0.46 0.08 14 ND 0.0002 ND ::3 1:D ti3 ND ND 0.08 liD 1ZD l:D 1ZD ND ND ND 1iD ND - -. ____-__-- Notes: 1. T h e p~ vas measured in a control test vessel at the start of the test. Hardness, alkalinity, total organic carbon, and residual chlorine were measured in dilution water at the beginning of the toxicity test. rota1 phosphorus. nitrate, chloride, fluoride, metals, pestim-ide, and PCB data were collected during February, 1995, as part of routine biannual water quality'testing (these analysis were per.tormed by Pace, Inc., Hampton, New Hampshire). 2. ND = not detected at or above the detection litnit. T . R . Hilbury Study Number 840-TH .. Page 9 of 22 i BACK TO MAIN ..' The original culture was obtained on June 1, 1994 from Aquatic Research Organisms, Hampton, New Hampishire. neasurements made during the 7 days before the start of the test indicated that the culture temperature ranged from 20.5 to 2I.O.C and the dissolved oxygen concentration was always at least 8.0 mg/L. The culture produced young prior t o day 12 and a subsample of adults produced at least 3 young/daphnid/day for I days before the start of the test. . .. During acclimation daphnids were not treated for disease, they were free of apparent sickness, injuries, and abnormalities at the beginning of the test and there was no mortality during the 4tl hours preceding the start of the test. The culture was supplied with a yeast/trout chow mix (Lot# DC02) and the freshwater alga, selenastrum capricornutum, daily during acclimation. Daphnids were not fed during the test. Control . daphnids, blotted and weighed at the conclusion of the toxicity test, averaged 0.36 mg and the loading rate during the definitive toxicity test was approximately 14 mg/L. TOIICITI TESTING: A static screening test was conducted from Jun.8 f J to 16, 1995 with five concentrations of test substance and a d i l u t i m water control. Nominal concentrations of L-13492 were (1.10. 1.0, 10, 100, and 1,000 mg/L. After 4 8 hours of exposure there was 1'10% survival in the control and at 0.10, 1.0, and 1 0 ng/L, and 06 survival at 100 and 1,000 mg/L. The definitive toxicity test was performed fron June 27 to 29, 1995, according to T.R. Wilbury Protocol 840-TH (Acute T o r i c ~ ~oyf L-13492 T o The Daphnid, Daphnia magna). The protoc:ol, which was signed by the study director on June 14, 1995, is based on procedures of the U.S. Environmental Protection Agency (1993). A summary 0::the test system is presented in Table 2. The test was conducted at a target temperature of 20 z 1-c with five concentrations of test substance and a dilution water control. Nominal concentrations of the test substance were: 0 mg/L (control), 1 3 . 22, 36, 60, and 100 mg/L. A 100 mg/L stock solution was fomulated by combining 0.2 g test substance and dilution water in a Class A volumetric flask and adjusting the final water volume to 2,000 ml. A P P ~ O P ~ilIlll.o~tm~ts~ of chi- solution were added to dilution water in test vessels t o formulate test media without the use of a solvent. Twenty daphnidi Yere indiscriminate1y and equally distributed m a g two replicates of each treatment. The test was performed in 300 ml glass beakers that contained 250 ml of test solution (water depth was approximately 9 cm). Test vessels were randomly arranged during t h e 48 hour test (a random numbers table was used t o select the location of each vessel) and they were loosely coverud. T.'R. Wilbury Study Numher 840-TH Page 10 of 22 -- BACK TO MAIN Table 2. Test system summary for the toxicity test with L-13492 and daphnids, Daphnia magna. species: Daphnia magna i Age/size of Test organisms: q24 hours old Test Duration: 4 8 hours Test Vessels: 300 ml g l a s s beakers Test Media Volume: 250 ml Depth of Test Media: 9 cm Number of Replicate Test Vessels: 2 organisms per Replicate: 10 Temperature Range: 20.4-2C.iC Minimum Dissolved Oxygen: 8 . 5 mgl!. Photoperiod: 16 hourri light and 8 hours dark. Light Intensity: 20 footcandles Water Quality Measurements: Dissolv13d oxygen, pH,. telrperature, and conductivity daily in each test vessel. Hardness, total organic carbon, and alkalinity once in the coctrol. Residual c!tllorine daily. Dilution water analysiis is suconarized in T a b l e 1. End Points: 24 and 4 8 hour LC50 6:nd EC50 (based o n immobilization), 4 8 hour no oDserved effect level. Acceptability Criteria: s l o p control mortality and acceptable temperature range. T.R. Wilbury study Number 840-Tki Page 1 1 of 22 BACK TO MAIN A 16 hour light and 8 hour dark photoperiod with e 15 minute transition period was automatically maintairied vith cool-white fluorescent lights that provided a light intenrnity of 20 footcandles. Aeration warn not required t o maintain dissolved oxygen concentrations above acceptable levels and daphnids were not fed during the test. The number of surviving organisms and the occurrence of sublethal effects (immobilization. loss of equilibrium, loss of reflex, excitability, discoloration, or change in behavior) vere determined visually and recorded initially and after 24 and 48 hours. Dead test organisms were removed when first observed. Dissolved oxygen (YSI nodel 57 meter; instrument numbers 1). pH (Beckman model pH1 12 meter; 'instrument number 1 2 4 ) , conductivity (cole Fanner meter, instrument number 9 4 1 , and temperature (Beckman model pH1 1'2 meter: inst1:ument number 124, prooe pHT-1) were measured and recorded daily in each test chamber that contained live animals. The temperature in a beaker of vater incubated among the test vessels was recorded continuously during th? test. STATISTICAL HFTEODS: Results of the toxicity test were interpreted by standard statistical techniques. Computer methods (stephan, 1983) were used to calculate LC5Os and EC50s. when possible. All calculations were performed by the auihor using nominal concentrations of test substance and the number of live or unaffected daphnids. The EO observed effect concentration is the highest concentration of test substance that did not cause any toxicant-related mortality or sublethal effects. T.K. Hilbury Study Number 840-TH Page 12 of 22 BACK TO MAIN I n s o l u b l e material was not noted during the terrt. Biological and water quality data generated by the acute toxicity teist are presented in Table 3 and Appendix A, respectively. The measured concentrations of dissolved oxygen ranged from 8.5 t o 8.8 mg/L (mean = 8.6 ag/L), the temperature ranged Zrom 20.4 to 20.7-C (mean = 20.S.c), conductivity ranged from 620 t o 640 pmhos/cm (mean = 630 pmhoslcm), and pH ranged from 8.4 t o 8.6. One hundred percent survival occurred in the control exposure and these daphnids did not exhibit any sublethal effects. The 24 and 48 hour LC50s and ECSOs for daphnids exposed to L-13492 are presented in Table 4. The 48 hour LCSO for daphnids exposed t o L1'492 is 77 mg/L (95% confidence interval = 6 0 to 100 mg/L) and the 48 hour ECSO is 34 mg/L (950 confidence interval = 30 t o 39 mg/L). The slope of the 48 hour dose response curve (based on mortality and immobilization) is 9.4. The 48 hour no observed effect concentration (NOEc) is 13 mg/L. T.R. wilbury Study Number 840-TH Page 13 of 22 .- BACK TO MAIN Table 3. survival and sublathal effect data from daphnids, Nominal concentration Daphnia magna. and L-13492. - - Number alive! of L-13492 0 24 4 8 (mg/L) Rep. hr hr hr the toxicity test with -Nunlber affected 0 24 40 hr hr hr 0 (control) 1 10 10 10 2 10 10 10 0 0 0 0 0 0 13 1 10 10 10 0 0 0 2 10 10 10 0 0 0 22 1 10 9 9 0 0 0 2 10 10 10 0 0 0 36 1 IO 10 9 0 0 6 2 10 10 10 0 0 4 60 1 10 10 9 0 0 9 2 10 10 a 0 0 8 100 1 10 10 3 2 10 10 0 0 0 6 e 3 - Note: ____ -- _ I Affected daphnids were immobilized. T.R. Wilbury Study Number 840-TH BACK TO MAIN ---- ......... ......... . . ...... -..... . .CC --- . I-.. . . . . . . . . . -. . . . . . . + 13 . . . .. . -. -7 -9 . .. . .. 36 -. I + + 60 iO C .... Figure 1. S u r v i v a l and s u b l e t h a l effr!cts of daphnids, Daphnia magna, exposed to L-13492 for 4 8 hours. T . R . wilbury Study Number 840-TI4 Page 15 of 22 BACK TO MAIN Table 4. Uedian lethal concentrations (LCSOs) and rnedian effective concentrations (EC50s) from the toxicity teat vith daphnids, Daphnia magna, and L - 1 3 4 9 2 . Exposure period LCSO 95 percent LC5 0/EC5 0 confidence limit P - Calculation method 40 hours kZ50 24 hours 4 8 hours 77 mq/L 09 mg/L 34 mg/L 60 to 1 0 0 mg/L Binomial 60 to >lo0 mg/L 30 to 39 mg/L Binomial Probit P a q e 16 of 22 BACK TO MAIN stephan, C.E. 1983. Computer Program for t h e Calculation of LCSO Values. U.S. EPA. Duluth, HN. Personal Communication. U.S. EPA. 1993. Toxic Substances control Act Test G u i d . e l i n e s : Final Rules. section 797.1300. U . S . EPA. 1993. 40 CFR Part 792. Toxic :;ubstances COtltrO1 Act (TSCA); Good Laboratory practice standards. Code of Federal Regulations, Title 40, July 1, 1993. T.R. Wilbury Study Number 840-TH Page 17 of 22 BACK TO MAIN Jeanne P. nagaiu,' study Director . Aquatic Toxicologist Robert L. Boeri coauthor Aquatic 'roxicohqist ' : I t * Io_, , , , t j 1, 7 -c FO? Jacqueline k. 'kevius Aquatic Toxicologist Biolcgisl: Peter C . taevius Biologist T.R. wilbury Study Number 840-TH Page 18 of 22 BACK TO MAIN 111. PROTOCOL A)(w(DHCl(TS AND DCVIA'PIOI(S PROTOCOL AUENDWITS: T h e protocol was amended t o specify the nominal concentrations for the definitive test and to correct a typographical error that stated that test vessels would be 300 ml beakers containing 250 liters o f solution (rather than 250 ml). P C O C O L DEVIATIONS: No deviations were made from the protocol. T.R. wilbury study Number 8 4 0 - ~ H Page 19 of 22 BACK TO MAIN Appendix A: WATER QUALITY DATA FROM TOKICITI T E S T Page 2 0 of 2 2 BACK TO MAIN Table A.l. Dissolved oxygen concentration, conductivity, pH, and temperature measured during the toxicity test w i t h daphnids, Daphnia magna, and L-13492. Nominal concentration of L-13492 (WL) Rep. Dissolved oxygen - (mg/L) 0 24 48 hr hr hr - Conductivity (phos/cm) 0 24 48 hlr hr hr 0 (control) 1 2 8.7 8.6 13.5 8.7 8.6 19.6 ;'I 1 8.7 8.6 13.6 2 8.7 8.7 13.6 22 1 8.7 8.6 3.5 2 8.7 8.8 3.5 36 1 8.7 8.7 8.5 2 8.7 8.7 8.5 60 1 8.7 8.7 8.6 2 8.7 8.7 8.5 100 1 8.7 8.7 8.5 2 8.7 8.7 8.5 630 630 630 630 630 6:30 630 6.30 630 630 640 640 620 620 620 620 620 620 620 620 620 620 620 620 630 630 630 630 630 630 630 630 640 640 640 640 T.R. wilbury study Number 840-TH Page 21 of 22 BACK TO MAIN Table A . l . continued. .. ~~~~ Nominal concentration of L-13492 (mg/L) ~ ~~ -~ ~ PH Rep. 0 24 hr hr ~~ - 4 1 hx Temperature - 0 24 hr hr (`c) 48 hr 0 (control) 1 8.5 8.6 8.4 2 8.5 8.6 8.5 13 1 8.5 8.6 8.5 2 8.5 8.6 8.5 22 1 8.5 8.6 8.5 2 8.5 8.6 8.5 36 1 8.6 8.6 8.5 2 8.6 8.6 8.5 60 1 8.6 8.6 8.5 2 8.6 8.6 8.5 100 1 8.6 8.6 8.5 2 8.6 8.6 8.5 ____-- - __- 20.6 20.6 20.6 20.5 20.6 20.6 20.6 20.6 20.6 20.6 20.6 20.6 20.5 20.5 20.5 20.5 20.5 20.5 20.4 20.4 20.5 20.5 20.5 20.6 20.7 20.5 20.5 20.5 20.5 20.5 20.5 20.5 20.5 20.4 20.5 20.5 I .,. T.R. Wilbury study Number 840-TH Page 22 of 22 04/01 /2001 ._ ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT 1 BACK TO MAIN LAB REQUEST NO. N2332 REQUESTOR NAME: SAB/S.J.MARTIN DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 06/07/1995 DESC: L-13492 ECOTOXICITY EVALUATION CONTRACT LAB(:S): TRWIL EXP COMP DATE: 08/30/1995 DATE COMPLETED: 09/ 02/ 1995 PRO4ECT LEAD : PHONE NO: 3M FAX NO: 651-778-6176 Tests conducted f o l l o w i n g 4OCFR 797; methods 1050, 1300, 1400. Sample desc.: L-13492, 45% s o l i d s , from S. M a r t i n . SAB 6/7/95 Sample i s a c l e a r l i q u i d . L o t confirmed as L o t #2 as per S.J. M a r t i n , o f 3M SCD Lab on 7/18/95. SAB Results f r o m 48-hr Daphnia magna acute t o x i c i t y t e s t : Test Conc., mg/L 0 (control) 13 22 36 60 100 Percent Immobilization 24-hrs Exposure 0 0 5 0 0 44-70 and/or Mortality 48-hrs Exposure 0 0 5 55 100 100 sab 7/31/95 Results from 96-hr Fathead minnow acute s t a t i c t o x i c i t y t e s t : Test Conc. mg/L 0 (control) 130 220 360 600 1000 24-hrs exp 0 0 0 0 0 50 Per cent M o r t a l i t y 48-hrs exp 72-hrs exp 0 0 0 0 0 0 0 0 0 0 60 70 sab 7/31/95 Results from 96-hr algae growth i n h i b i t i o n t e s t : Test conc. mg/L 1.o 2.0 4.0 8.0 16 EC50 95% C . I . sab 9/1/95 24-hrs 0 44 16 22 63 15 (9.8 - exp. >16) Percent change from 48-hrs exp. 2 17 17 42 51 14 (8.2 - >16) Control 72-hrs exp. -2 27 38 55 61 7.1 (4.7 - 11) 96-hrs exp 0 0 0 0 0 70 96-hrs exp. 0 30 50 56 80 4.9 (3.5 - 6.7) 04/01 /2001 * = CONTRACT LAB DATA ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT BACK TO MAIN 2 1 05/04/95 L-13492 Lot 2 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0 * = CONTRACT LAB DATA 34 MG/L 890 MG/L 4.9 MG/L ( 3 0 - 39) (600 - >1000) (3.5 - 6.7)