Document ymL8w68R6r23eomwwYzMoGvKE
,)'HAZLrm%CN LABCNDMMRIEB AA/ERICA, INC.
3301KINSMAN BLVD. p.o.BOX 45 MADISON, WISCONSIN 53707
Chemical& BioMedicalSciencesDivision
PHONE (608)241-4471 TLX 703966HAZRAL MDS UD
FINAL REPORT
JUL,
JANINE GLEASON MINNESOTA MINING
& MANUFACTURING
TOXICOLOGY SERVICES ST. PAUL, MN 55101
COMPANY
SAMPLE: T-3752 PURCHASE ORDER NUMBER: T357842, REL. #513
SAMPLE NUMBER: SAMPLE ENTERED: REPORT PRINTED:
50503501 05/15/85 06/21/85
ENCLOSED:
PRIMARY EYE IRRITATION QAU REPORT RAW DATA APPENDIX
- METHOD,
SUMMARY
SIGNED:
STEVEN M. GLAZA STUDY DIRECTOR ACUTE TOXICOLOGY
........
DATE
..........
BY AND FOR HAZLETON LABORATORIES
AMERICA, INC.
RAW DATA FOR THIS STUDY ARE KEPT ON FILE AT HAZLETON AMERICA,, INC., MADISON, WISCONSIN.
LABORATORIES
HAZLETON
LAEK3FmW%3RIr=S AKAERICA. lfJC.
3301KINSMAN BLVD. o P.O.BOX 7645 MADISON, WISCONSIN 53707
Chemical& BloMedicalSciencesDivision
PHONE (606)241-4471 TI.@703956HAZRAL MDS UD
SAMPLE NumeER: 50503501 SAMPLE: T-3752
PAGE
2
OECD EYE IRRITATION
Objective: To determine the level of ocular irritation produced following a 5inale exposure of a test substance to one eye of albino rabbits accoriing to the Organisation for Economic Cooperation and Development,5 Guidelines for Testing Chemicals, Section-405, Acute Eye Irritation/ Corrosion, adopted May 12, 19Bl.
Test Material: T-3752 Physical Description: PUrity and Stability:
Brown granular solid Sponsor has purity and stability
on file.
determinations
Test animal: Young adult rabbits (approximately 14 weeks of age) of the New Zealand White strain were procured, maintained individually in screenbottom cages in temperature- and humidity-controlled quarters, provided continuous access to Purina High Fiber Rabbit Chow and water, and held for an acclimation period of at least 7 day5.
Three acclimated animals, weighing from 2333 to 2504 g, were chosen at random for the test. The animals' eyes were examined within 24 hours prior to test material administration using sodium fluorescein dye procedures. Only those animals with no sign of ocular injury or irritation were used. Te5*t animals were identified by animal number and corresponding ear tag.
Reason for Species Selection: The New Zealand White albino rabbit is the animal of choice based upon its large orbit and nonpigmented iris.
Preparation and Administration of Test Material: The sample was dosed as
received. A bulk density determination was made to determine the weight equivalent of a 0.,1 ml do5e. Because individual d05e5 should not exceed 0.10 g and the weight equivalent of 0.1 ml was 0.12 g. an individual dose
of 0.1 g was weighed out for each animal.
Treatment: Each rabbit received 0.1
everted lower lid of one eye, with
the untreated control. The upper together for one second to prevent
g of the test material placed on the the contralateral eye serving as and lower lids were gently held 1055 of material and then released.
The eyes Of the rabbits remained unflushed.
HAZLC%CN
LAM3P4=C3RIEB ANAERICA. INC.
Chemical& BloMedicalSciencesDivision
3301 KINSMAN B@VD. a P.O.BOX 7545 0 MADISON, WISCONSIN 53707 o PHONE (608)241-4471a TLX 703966HAZRAL M DS UD
SAMPLE NUMBER: 50503501 SAMPLE: T-3752
PAGE
3
OECD EYE IRRITATION
(CONTINUED)
Observations: The treated eyes were observed for ocular irritation at 1, 24, 48, and 72 hours after treatment.
At the 72-hour reading, sodium fluorescein was used po5sible corneal injury. Irritation was graded and to the Draize* technique.
to aid scored
in revealing according
'Animal5 were weighed just prior to test material admini5trat.ion.
Pathology: At study termination all animals were euthanatized and discarded.
*Draize, J.H., "Apprai5al Of the Safety of Chemicals in.Food5, Drugs, and Cosmetics - Dermal Toxicity." Association of Food and Drug Officials
of the U.S., Topeka, Kansas, pp. 49-51 (1959).
(OHAZLC@CCN
LABCW4=rCRir=S ARAERICA. INC.
3301 KINSMAN BLVD. o P.O.BOX 7545 MADISON, WISCONSIN 53707
Chemical& BioMedicalSciencesDivision
PHON,- (608)241-4471 TLX 703956HAZRAL MDS UD
SAMPLE NUMBER: 50503501 SAMPLE: T-3752
PAGE
4
OECD EYE IRRITATION
SUMMARY
(CONTINUED)
Test Animal:
Albino
rabbits
- New Zealand
White
Source:
Hazleton Research Products,
Inc... Denver PA
Date Animals Received:
05/21/85
Temperature
and Humidity of Animal Room:
21 to 23 Degrees C.;
46 to 64% Relative
Humidity
Test Material:
T-3752
Date Test Started:
05/29/85
Date Test Completed:
06/01/B5
PRIMARY
EYE IRRITATION
OBSERVATION
PERIOD
1 Hour: 24 Hours: 48 Hours: 72 Hours:
SCORES*
3 Rabbit Mean 0.1 g
(Unwashed)
10.7 0.0 0.0 0.0
The Primary for all the observation
Eye Irritation animals divided period.
Score is the total eye irritation by the number of animals (3) at
score each
Comments:
No pain response
following instillation of
(vocalization)
was
the test material.
elicited
from any animal
No corneal irritation was observed during the study.
HAZLE%CN
LMX3RM'ORIES
ANALCRICA. ""C.
3301 KINSMAN. BLVD. -P.O. BOX 7645 MADISON, WlscONSINS3707
Chemicai& BioMedicalSciencesDiviIson
PHONE (608)241-4471 TLX703956HAZRALMDSUD
SAMPLE NUMBER: 50503501 SAMPLE: T-3752
OECD EYE IRRITATION
Animal Number F08211
F08705
F08721
Observation Period
1 Hour 24 Hours 48 Hours 72 Hours
1 Hour .24 Hours 48-Hours 72 Hours
1 Hour 24 Hours 48 Hours 72 Hours
PAGE
5
(CONTINUED)
Table I Individual Eye Irritation Scores
Cornea.
A
8
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
a
0
0
0
a
0
0
0
0
Score AXBX5
0 0 0 0
0 0 0 0
0. 0 0 0
Iris Score
A
AX5
1
5
a
0
0
0
0
0
0
0
0
0
0
0
a
0
1
5
0
0
a
0
0
0
Conjunctivae
A
8
c
1
2
0
0
a
0
0
a
0
0
0
0
1
1
a
0
0
0
0
0
0
0
a
0
1
2
3
0
0
0
0
0
0
0
0
0
Score (A+B+C)2
6 0 0 0
4 0 0 0
12 0 -0 0
Table 2 Sodium Fluorescein Examination
Animal Number F08711 F08705 FOB721
Observation Pre-initiation
NEG NEG NEG
Period 72 Hours
NEG NEG NEG
NEG - No stain retention POS - Positive stain retention (area of cornea involved).
References:
1. Organisation for Economic Cooperation and Development's Guidelines for Testing Chemicals, Section 405, Acute Eye Irritation/Corrosion, adopted May 12, 1981.
2. Draize J.H., "Appraisal of the Safety of Chemicals in Foods, Drugs, and Cosmetics - Dermal Toxicitv"@ Association of Food and Drug Officials of the United States, Topeka, Kansas, pp. 49-51 (1959).
PAGE 6
QUALITY ASSURANCE STATEHENT Primary Eye Irritation Study in Rabbits
Study No. 50503501
The report as herein attached for the above--mentionedstudy has been reviewed
by the assigned Quality Assurance Unit of Hazleton Laboratories America, Inc. in accordance with the Good La*ooratoryPractice Regulations as set forth in 21 CPR @8'.35(b) (6) (7). It has been found to accurately identify and/or describe the authorized methods and standard operating procedures followed in the conduct of the study,and that the reported data accurately reflect the raw data of the laboratory study. Furthermore, the Quality Assurance Unit has conducted the following inspections of the testing-facilitiesutilized in the conduct of this study and has submitted written reports of said inspections to
the study director and/or management.
Date of Inspection 5/21-23/85 6/10/85
Type of,lnspectiotiProcess Audit Report Review
Date Issued to Management 5/23/85 6/10/85
Diana 1. Skalitzky/ Inspector, Quality Assurance Unit
Date
PROTOCOL
ATTACHKENT 1
c1)
(A) g2UL= degree of density (area =at dense t&k*ftftr r*'Aiig)
No ovwitl
Soattered or dlrrom u-co, astau& of Lris olezvly visible
zagay 4&mernlble tranjuawt are", 4*taus of irsa
supul ob"urod opatemewtare". FA details or LrU vi"bJLe. 3:LZGOf Pug" bw*Ly dineraLbL@
Lns IMLAble
Cl) AM At M=24 12=LUA
fte 4@ww (w 10"), but nt two
amter thm ow quI a~. ftt low thm "L.
2
3
anatw ma air# m I*" umm ti1r"quartws
4
Grmt4r tum thm luwtomo up to UML*-",Oa
A
TouL ftdma
(2)
CA) JB2=
Norm" nkum abme no@L. ooogwtlm.
gweulm. eirwAwom"L
"JoetL"
an at %bon or embimuse of my thomf) LIU altu
Ugn (&Luazo remtlm is Po"tLFG)
go reastim %toot
gqm *wu-vntL= Calior au
se tbwo -
az
?SUL ULTIOM a 10
(3) S06USSUM
(,%)isg= voom" TOMMLS 6*CLNL&47 Uooes" &berm MIS"
lum diftwes "@FitW"o" P". ulaftew"a""a sell-
om"y dumaime -- - 3
ft meuuw Any MOLUMC abvl@
SMOLUM &A"un
so*"
OWML
cimlades
sistitating
seowwe)
sw*LLin idta pu-A" *we-"= of Lids
with Uds &be" W" "D"d %its LWA abmt mLf SLOO" to oml.@Ldly
(C) xacham
ft dlasbarp -
- ----
iwwme=& autsfts n-m awn" (do" insUNLU" Mau amewts
ebm I is Lamr Matmw of mro" saim&La) nommwp wItAanutoulas of the U" UM balrojust&djoomt
toLt"
--
2
Koomw
witil gg"tmln
of tim UA& md baift, Ut *G"LdOmbit
anwd tbo an
Swm CA
C) 2
Tota H&SI@ 20
IM 'at" men ftr the S" Is =4 am of &U Saw"
for me
awass. Lrut am *=JGOG*-Lv"-
Primary EYe Irritation Test Tnitial Sodium Fluorescein Lcan and Animal Body Weights
Test CO=Pound 7-- -3
PH Result
Dose (9) --O.fr4
Dose (al).
Aliq
Date Animals Received
sourr-4: H4zlttoli
Animotl$0. Sez SP
ZvIoLczaai@iz
"tim follovin
RT NO.
Room No.
]@onedBy
Date
/19
Reviewed BY pine," Date
f.
An4mnl Body :Lzhts(n)
Initiation Day 7
Day 14
Day 21
Al
Q333
qQ 3L
:?
TECHNIC,"
Limza
ck
NCORDED k
DATE /f IJ6 5/-6q
SCALE USED
Sodium Fluorescein
NEC - Negative
POS - Positive
NA n Not Applica.ble y a Yes N a No
WZN"4nmt:LCCL
0,@e
0061-075
lo' qSilM
it
Primary Eye irritationTest Observations
Test Compound-rT*st Eye
Washed
RT NO.
Group
Seconds followinginstillationof test
materials the test eye wits Washed with
Alh al of am@A/14
for AI& second$
Unwashed
inal No./ r Tax No
Eg-.090
EO rcuactailoLoenfsions
ail
Head
cular Structure:
orne.a - Opacity
Area
0
iris
Conjunctivas
Redness
chalwais
Discharge
Sodium Fluorescein EX"
achnician
corded By
ate.
A a Purulent Discharge @B - Clear Discharge C a Petits gmorrbage D a Blanching INJ a Injected MEG a Negative POS - Positive
Reviewed By:
OBSERVATION PERIODO
0
C)
1'-ncr C)
2319
Al
C9
9 Corneal Spithelial Damages Peeling IPa.Corneal Epithelial Damages Piling G a corneal Epithelial Damages Pitting
R*- Pannue I - Corneal Naovascalarization MA u Not Applicable
Data:
Eye Irritationsearig:o t 4li3 1-A17
Primary Eye Irritation Test Observations
Test Compound Test Eye
Washed
RT NO. 50S035-401
Group
Seconds followinginstillationof test
material, the test eye was washed with
A.14 al of A/04
for Al,# seconds
Umashed
No./
r Tait Moe cation of r"nrTantaFaalailLoensLi:lolns
il -C
cular Structure ornes 22&city___
Area
Iris
Conjunctivae
Redness
Chemosis
Discharr,e
Sodium Fluoreseeinim Technician
fY2
corded ly
Fa>o
A a Purulent Discharge 3 a Clear Discharge C a Petit* Smorrhage D stanchids INJ Injected MG Negative POS Positive
Reviewed By:
OBSERVATION PERIOD:
dR *C-O I
hota V'
I Corneal Spith*lial Damage, Peeling
F =Corneal EpitbalialDamageg Piling C - Corneal Epithelial Damaga,,Pitting
R'-.Pannus I - Corneal Neavascularization MA a Not Applicable
Dots:
Eye Irritation$core: Di. (1311A)
Primary Eye IrritationTest Observations
Test Compound Washed
itNro.5'-0543@5-101
Group
Ale-
Seconds followinginstillationof test
material, the test eye was washed with
Alh al of .- Alft
for Altt second$
Unwashed
u,oaicFs&tai No;/ ar Taag no*
ortaueatliLoeifn
rue'ailoNnL*ae*'sions
OBSZRVATION-PERIOD:
Lioo iioL4
00*'+/
-.?-C-;
ail c.
-
lar Structure
rcooriunrea
opacity-
Area
iris onjunctivae Redness Chemosis
Dia_charjte
Sodium Fluorescein Zxm
Technician
corded Sly
CK
taate /4-65' 5-31
A Purulent Discharge Clear Discharge
cl Petite Hmorrhass
D Blanching
INJ
Injected
MG a Negative
M u Positive
(--Kl 3)
C-k-
9 o Corneal Epithelial Damage* Peeling r a Corneal Epithelial Damages Piling C a Corneal EpitI balial Damage, Pitting R*o Paums I a Corneal Neovascularization MA a Not Applicable
Reviewed By: *,vex
Date: 10-5%5 Eye Irritation$core:
(1311A)
.PrimaryEye IrritationTest Observations
Test Compound-r-
Toot Eye
Alq
Washed
ILTRoo
Group
Al&-
Seconds followinginstillationof teat
materiaa,l,of @the test eye was washed with Alft for 4g@&.second#
Vinwaabed
=I
No.1
orrrr-ta'TTfmrtaaattnlooif.ou*
rneal Lesions
OBSERVATION PERIOD:
L)LA
ailg.
>AAA
culSatructure
ornes Opacity
Area
Iris
onjunctivae
Redness
chmwsis.
Disobarr,e
Sodium Fluorescein Exas
echnician
4-otm
ZMNOM
corded By-
@arat*
A a Purulent Discharge 3 a Clear Discharge C m Petite neworrhag* D Blanching
Injected a,NcJ negative POS Positive
Za 7s 0R*w In
NA a
Corneal Zpithelial Damtgo Corneal Spithelial Damgeo Corneal Epithelial Damage, Paums Corneal Neovascularization Not Applicable
Pealing Piling Pitting
Reviewed By':
Dates
Eye Irritation$care: o-to (1311AY
HAZLra%CNLABORATORIES 3301 KINSMAN BLVD. P.O.BOX 7545
AMERICA, INC. MADISON, Wl 53707 (BD8)241-4471
TLX 7039M HAZRAL MDS UD
PROTOCOL TP2072
Primary Eye Irritation Study in Rabbits (OECID Guidelines)
Study No. 50503501
for 3M St. Paul, Minnesota
by Hazleton Laboratories America, Inc.
Li-fe Sciences Division 3301 Kinsman Boulevard Madison, Wisconsin 53704
May 21, 1985 1985, Hazleton Laboratories America, Inc.
Chemical & BioMedical Sciences Division
PROTOCOL TP2072
Primary Eye Irritation Study in Rabbits (OECD Guidelines)
Study No. Study Location
Test Material Sponsor's Representative Study Director
50503501
Hazleton Laboratories America,
Life Sciences Division 3301 Kinsman Boulevard Madison, Wisconsin 53704
Inc.
T-3752
Janine Gleason Steven M. Glaza
Proposed Timetable Starting Date Completion Date Final Report Date
Week Week Week
of May 27, 1985 of June 3, 1985 of June 24, 1985
OBJECTIVE The objective of this study is to determine the level of irritation produced following a single exposure of a test material to one eye of albino rabbits. All aspects of this study will conform to the Organisation for Economic Cooperation and Development's Guidelines for Testing Chemicals, Section 405, Acute Eye irritation/Corrosion, Adopted May 12, 19811 and the Principles of Good Laboratory Practice. 2 All procedures will be done according to Razleton Laboratories America, Inc. (HLA) Standard operating Procedures (SoPs) referenced in this protocol.
Identification Test Material: Physical Description: Purity and Stability:
Storage Conditions: Test Material Retention:
Safety Precautions-.
TEST MATERIAL
T-3752.
Brown granular solid.
Sponsor has purity and stability determinations on file.
Store at room temperature.
Any unused test material will be returned to the Sponsor 30 days after issuance of final report.
Laboratory personnel will take the normal necessary precautions in handling a substance of unknown toxicity. Laboratory clothing, latex gloves, safety glasses, and a particle mask approved for toxic dusts must be worn*
2
TEST SYSTEM Test Animal
Young adult albino rabbits of either sex of the New Zealand White strain, approximately 14 weeks of age, will be obtained from Hazleton Research Products, Inc., Denver, Pennsylvania. An adequate number of extra animals will be purchased so that no animal in obviously poor health is placed on test. The New Zealand White albino rabbit is the animal of choice based upon its large orbit and nonpigmented iris.
Acclimation Upon receipt, the animals will be taken to a designated animal room'where
they will be acclimated for at least 1 week before being placed on test (OP-GENB 36). During acclimation, the animals will be examined for clinical abnormalities indicative of health problems (e.g., diarrhea, ectoparasites, rough hair coat, nasal or ocular discharge, evidence of injury, etc.). Any animals regarded as unsuitable for the study purposes because of poor physical condition will not be released from acclimation and the reason(s) will be documented.
Identification Each animal in the study will be assigned a pe*rmaneutidentification
number and will be identified with a metal ear tag (OP-GENB 24). All data collected from an animal will be recorded*and filed under its identification number.
3
Housing and Maintenance The following environmental conditions will be maintained in the animal
room used for this study (OP-TARC 230).
0
Temperature: 21*C +20
0
Relative humidity: 5OZ +20%
0
Air change: At least 10 changes an hour of filtered 100% outside air
0
Light cycle: 12 hours light/12 hours dark
Temp erature and humidity will be monitored throughout the study. Variations
from prescribed environmental conditions will be documented.
Animal husbandry and housing at IUA comply with standards outlined in the "Guide for-the Care and Use of Laboratory Animals.lt3 Care will be taken to ensure that the animals are not disturbed for reasons other than data collection and routine maintenance. The animals will be housed individually
in screetr-b.ottomstainless steel cages (heavy gauge) held on racks, with
absorbent pan liners in the urine- and feces-collecting pans. Pan liners will
be changed at least three times each week.
Feed and water will be provided ad libitum. The diet will be Purina High
Fiber Rabbit Chow. No contaminants are expected to be present in the feed or
water which would interfere and affect the results of the study.
Study Design Three rabbits will be selected at random based upon health and a body
weight of 2.0 to 3.5 kg.
PROCEDURES
Preparation and Administration of Test Material .
The rabbits' eyes will be examined using fluorescein dye procedures within 24 hours prior to test material administration. only animals with no sign of
4
corneal injury or eye abnormalities will be utilized. one eye of each animal 'Will be treated with the test material and the other eye will serve as the untreated control.
Each rabbit will receive 0.1 g (or the weight equivalent of 0.1 mL) of solid test material. If necessary, the solid test materials will be finely ground into a dust or powder. The test material will be placed into the everted lower lid of the rabbit's eye. The upper and lower lids are then to be gently held together for 1 second before releasing to preveat loss of material. The eyes of the rabbits will remain unflushed for 24 hours following instillation of the test material. After'24 hours, a washout may be used if considered appropriate.
Reason for Route of Administration 4
Historically, the route of choice based on the method of Draize.
Observations The treated eyes of all animals will be examiu*edfor ocular irritation at
1, 24, 48, and 72 hours after treatment. If no irritation or injury is present at 72 hours, the study will be terminated. If irritation is present
at 72 hours, additional observations will'be made at 96 hours and at 7, 14, and 21 days. If at any of these time points there is no irritation, the study
will be terminated. If injury is still present at 21 days, the Sponsor will
be contacted to determine whether the study should continue or be terminated.
After recording the 24-hour observations, sodium fluorescein may be used to
aid in revealing possible corneal injury. Irritation will be graded and.
4 scored using the Draize technique (Attachment 1).
All eye abnormalities
will be recorded.
5
All animals that have a damaged eye producing undue stress or discomfort will be sacrificed for humane reasons after consulting with the Sponsor.
Body weights will be recorded prior to test material administration and at weekly intervals throughout the study. Observations and body weights will be recorded in the study notebook.
Pathology All animals, whether dying or sacrificed at study termination, will be
discarded.
Report The final report will present a description of the test materials a
description of the-test system, dates of study initiation and termination, a summary table showing the irritation data at each observation period, and any special observations that were recorded.
Maintenance of Raw Data and Records Original data or copies thereof will be available at HLA io facilitate
auditing the study during its progress and prior to acceptance of the final report. When the final report is completed, all original paper data, as well as the final report, will be retained in the archives of HLA, Madison, Wisconsin (OP-GEN 44).
6
REFERENCES 1. "Ar-uteEye Irritation/Corrosion,"OECD Guidelines for Testing Chemicals,
Section 405 (May 12, 1981). 2. Organisation for Economic Cooperation and Development's Principles of Good
Laboratory Practice, Annex 2, 1981. 3. DEEW Publications No. (NIR) 78-23 (1978). 4. Draize, J. U. I Appraisal of the Safety of -Chemicals in Foods, Lku@gs, and
Cosmetics - DernaL_21!,-Lci_@iZi,isociatiouof Food and Drug otfic3.alsot the U.S.T,-opeka, Kansas, pp. 49-51 (1959).
7 PROTOCOL APPROVAL
&=n2'.IisrG'lseason Spons 'a aRRseepresentative 3M
AM-
Steven M. Glaza
U
Study Director
Group Leader, Acute Toxicology
Hazleton Laboratories America, Inc.
(1109s/tii)
Date
PROTOCOL
ATTACMMLQT 1
Carnes
(A) gRm=
_ d-lp 0 of density (grea =at dens* takn for rtsdiig)
No opacity &mttorse or dlrr"e area, detailsof iris clearly visible
gully dl=emible traulucent &ruse details of iris
silgittl"ySam-ed
2
opaennt areas, so detail&of iris visible, size of pup"
barely discernible '
3
ifts JMJ"ble
4
fte *Wtw
(w 10") 0 13111,not awe
I
Ormter them one quar%ort but team thm b&Lr
2
anatw tbm war, butIwo thanuw" qmrters
3
CrUtW
tbM tbPOO 4U&rt*Mo
UP to WbAaS'-&r*a
4
A x 5x 5
Total Recion 90
CA) lgl=
Norm" -
a
so- 8 MGM'
t conestlang
S"Ulms
cire%moom*sl
"Joe"=
a, &U of O*se or embuatim of =7 taer"r) LrL>=l re"tlftt@ Uof, (Slog"L reactionU positive)
ft re"tlm to ugm, beem""O' or tbe")
vv" liesu-"tim (alaior au
Total Maxima a 10
3) sanaugum
(A) It==
Crefw2 to pdpobral aftjmativao mir)
Tessou core" Teawas 4*fLnitoly Weated above norma
YAM dltfwst doopw arl&am red, LrAiv:Ldwa wesa*U not
eldly dLaawaMe
OLfftes""V r"
3
so M-Ouug
A" vioULM
above morsel (imlodu
nittLtatUg
asubruse)
I
Obviom wAsLUng with partial eversion or ]Ads
2
SweLLtag with Uds about war closed
3
3welLIAS idth Uta abe" Mlf alomm to ampletely alomw
4
(0) aLaODAM so discharp Aar asomt dLf.Oarmt f@4= norwa (does not include small amunts
obawv" In Umw owthus of normal salods) oisobam w:Ltasoistaninigor the uds and blire just adjoatut to Uds Disebarp With maistanim or the Li" md h&Lra, md emst4wable arm arowd the we
Score (A*B*C) xt
Tat" Nuiam a 20
The tot" som-9 ftr the eye is tan am or &U *earn obtained !or the
