Document ykQBYzqkgeG9rxrb8B3GXd4wE

AR 886 -- 074% ACUTE TOXICITY TO FISH (FATHEAD MINNOW) EST SUBSTANCE Identity: AbemriexfteurrreedcotnotaaisniPnFgOpSe,rfFlCu-o9r5o,ocotranaessaifcoonamtpeo,newnhitcohfmFaCy-2a0l3soC and L-5440. (1-Octanesulfonic acid, s1a,l1f,,2C,2A,S3,#3,42,749,55-,359,-636),7,7,8,8,8-heptadecafluoro-, potassium Rsaemmpalreksis: FC-T2h0e3C3Manpdroidsurcetfieornreldottno ubmybtehre wtaesstinnogtlnaobtoerda.torTyhaest*e8s1t-1/8". C6u0r%rewnatteirn,fo3rm0a%tidoineitnhdyilceanteesgltyhcaotlFbCut-y2l0e3thCeris, a3%mixstoudrieuomfo1c.ty4l7s%ufPaFtOeS,,3% hydroxy sodium lfaouraymlersu,if1a0te%, paonldyo0x.y0e5t%hybleennzeotmroinazooolc.tylphenyl ether, 0.12% Tcohnecefnoltlroawtiinognosfumimmpaurriytiaepsp.liDeasttaomaawyitnhoitnaccocmuprlaetteellyyrcehflaercatcttoexriiczietyd of the fluorochemical componoeftnhtetestsample... METHOD: Method: EG&G, Bionomics protocol entitled "Methods for conducting fTleoswt-tthyrpoeu:ghFltoowxi-ctihtryotuegshtsawciutthefreshwater fish. GLP: No YSepeacriCeosm:pPliemteepdh:al1e9s81promelas ASnuaplpyltiiecra:lCmoomnmietrocriianlg:suTpepmlpieerraitnuNreeb,rpaHs,kaDO, and conductivity Exposure Statistical pmeertihodo:ds9:6-LhoCusrsvalues calculated using the computer program (prcorbeiatteadnablyysSist,epohrabni,no1m8i7a8l)pbraobsaebdiloitnytahseampopvriopnrgiaatvee.rage angle analysis, ~. Test fish age: Not noted. Length and weight: LWeeniggthh=t= 303.2--5 5--41m.m53gwiwtihthaamemaenaonfo4f10.m57mg LProeatdrienagt:me0n.t3:8 gNiolt noted Test coDnidliuttiioonnsw:ater: Well water supplemented with TownofWareham uDinlturteiaotnedwaantedrucnchhelmoirsitnrayt:ed well water. Hardness: 22 - 24 mglL (as CaCOs) 001008 Alkalinity: 18-24 mg/L (as CaCOs) Conductivity: 90 umhos/cm Lighting: pH: Cool White 67-68 fluorescent lights provided a daily photoperiod of S16tohcokurasndligthetsatnsdol8uthioounrsprdaerpkarwaittihona:n iAntpernsiimtayroyfs3t0oc--k 7so0luftoiootncoafnd7e8s0. `mwgat/emrLanwdasdiplurteipnagrteod4b0y00admdLi.ngAl3l1t2e0stgsroalmustoifontsewsetrseampprelpeatroeddibstyialed diluter delivering five nominal concentrations of the test sample and cFolnotwro-lthwartoeurg.h rate: Approximately 6.3 volume replacements of test water every 24 hours. Stabilityofthe test chemical solution: Not noted. Exposure vessels: 39 x 20 x 25 cm glass tanks which maintained test solution volume of 15 liters. Number of replicates: two - Number of Numberof fish per replicate: concentrations: 5 10 plus a blank control Water chemistry during the study: pH range (0-96 hours): 6.97.1 (control) 6.87.2 (2000 mg/L) `Temperature range (0-96 hours): Dissol2v1ed o2x2ygCe(nforraalnlgaequ(a0r-i9a6)hours): 68..2498..14 mmgg//LL (control) (2000 mg/L) RESULTS Nominal concentrations: Bk control, 125, 250, 500, 1000, and 2000 mgiL Eloment value: 96-hour LCso = >2,000 mg/L Element values based on nominal concentrations. Remarks: Testing was conductedon the mixture as described inthetest substance remarks field. The value reported applies to that mixture and not the fluorochemical portion alone. CONCLUSIONS bTehe>2F,C0-0200m3gC/L9.6-ThohuerhLiCgshoesftorcoPnicmeenpthraatlieosnptreosmteedl,a2s0w0a0smdge/tLe.rimnidnuecdetdo only 5% mortality. 001009 `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY EE --.,HHS PH tReesltiianbgi.litHyo:weKvieirm,istchherraeniksinngo 2a.nalTyhtiiscasltu(odryomteheert)sianlflotrhmeatciroitnertioacfoonrfqiuramlity thediluter was working properly. The study lacks information on the purity ofoftfhleuotreoscthseumibcsatlanincethaensdolluatcikosn.actual measurements of the concentration REFERENCES -- Tthheis3sMtuCdoywmpaasnyc,on3dMucLtaebd bRyeqEuGe&sGt,noufmbWearre7h03a8m,,1M9A81atthe request of OTHER Last changed: 6/26/00 001010 EAL # 8/2(8 LRPo. 703Y : ACUTE TOXICITY OF 81-1/8 TO FATHEAD MINNOW (Pimephales promelas). t : FC-203 cC . 1 ! TOXICITY TEST REPORT : SUBMITTED TO aN : ST. PAUL, MINNESOTA. | REPORT. $BW-81-10-1030 AquaticEGT&oGx,icBoiloongoymiLcasboratory ! Wareh7a9m0, MaMianssaScthrueseettts . October, 1981 001011 INTRODUCTION The purpose of this study was to estimate the acute toxicity of 81-1/8 to fathead minnow (Rimephales promelas) under flow-through conditions. Preliminary range-finding static tests and a subsequent 96-hour flow-through test were conducted at the Aquatic Toxicology = * | Laboratory of EG&G, Bionomics, Wareham, Massachusetts. The acute toxicity of 81-178 was measured by estimating the LC50 Value for | the compound at each 24 hour interval of the test. The LC50 is defined as the concentration of the test compound in dilution water which caused mortality of 50% Of the test-animal population at -the stated exposure interval. The flow-through test was conducted from 24-28 September 1981 with a two day pre-exposure period. ALL raw data generated are stored at the above location. MATERIALS AND METHODS Procedures used in this acute toxicity test closely followed | +' `those described in the EG&G, Bionomics protocol entitled "Methods for conducting flow-through toxicity tests with freshwater fish." Values are reported to different levels of significance depending on the accuracy of the measuring devices involved in any one process. The 81-1/8, a brown colored liquid, was received from 3M, St. Paul, Minnesota on 12 August 1981. The test material was stored in the _ dark at ambient temperature (21C). The material was tested as 100% 001012 2 R active ingredient. Nominal test concentrations are reported as milligrams of 81-1/8 per liter of test solution (mg/2). _ The fathead minnow (Bionomics lot #81A17) were obtained from a commercial fish supplier in Nebraska and held in a 500-2 fiberglass | tank under a photoperiod of 16 hours light and 8 hours darkness. ALL fish were fed a dry, pelleted food, ad libitum, daily except | during the 48 hours prior to testing.. There was 1.08 mortality in the test fish population during this 2 day period (Daily Record of Fish Holding Conditions). The well water which flowed into this tank was characterized as having total hardness and alkalinity ranges as calcium carbonate (CaCO) of 22-24 mg/i and 18-21 mg/%, respectively and a specific conductance range of 80-90 micromhos per centimeter (umhos/cm) (Weekly Gravity Feed Tank Water Quality Analysis Logbook). Other parameters monitored in the holding tank | were a pH range of 6.5-6.7, a dissolved oxygen (DO) range of 94-99% -- of saturation and a flow rate of 18 tank volume replacements/day I (Weekly Record of Fish Holding Water Characteristics).- Test fish | were maintained under these conditions for a minimum of 14 days. ~ k The `temperature in the holding tank was 21C during this 14 day period: (Daily Record of Fish Holding Conditions). The specific conductance was measured with a YSI Model $33 salinity-conductiyity- temperature meter and probe, the pH was measured with an mk Instrumentation Laboratory Model #175 pH meter and combination . electrode, the DO was measured with a YSI Model #57 dissolved 001013 3 oxygen meter and probe and the temperature was measured with a . Brooklyn alcohol thermometer. Total hardness and alkalinity were measured according to APHA et al. (1975). ) The exposure system used in this study was a modified, proportional | diluter similar to that described by Mount and Brungs (1967) with 2 0.50 dilution factor. The dilution water was well water which I was pumped to an epoxy coated concrete. reservoir where it was ` supplemented with Town of Wareham untreated and unchlorinated well | water and aerated. This water was characterized as having total hardness and alkalinity ranges as CacO, of 22-24 mg/2 and 18-24 ng/t, respectively, a pH range of 6.7-6.8 and a specific conductance Of 90 ymhos/cm (Weekly Gravity Feed Tank Water Quality Analysis Logbook.) | The diluter delivered five nominal concentrations of 81-1/8, = | 2000, 1000, 500, 250, 125 mg/% and control water containing no : 81-1/8 to duplicate test aquaria. Each glass test aquarium measured | 39 x 20 x 25 centimeters (cm) with19.5 cm high drains which maintained water volumes of 15 . The diluter delivered 0.5 of test water to each aquarium at an average rate of 188 cycles per ; day. This is equivalent to 6.3 aquarium volume replacements per ! 24 hour period. Illumination was provided by Cool WhiteR fluores- cent lights centrally located above the test aquaria. Sixteen hours of light at 30-70 footandles at the water surface were 001014 4 . provided each day. Test temperatures were controlled by immersion coil heaters in a water bath where the test aquaria were located. A 4-2 glass Mariotte bottle in conjunction with a "dipping bird" { delivery systen (Lemke, et al. 1978) was utilized to deliver a | stock solution to the mixing chamber of the diluter with every cycle. The stock solution of 780 mg/mi was prepared by adding 3120 grams of 81-1/8 to distilled water and diluting to 4000 mf. The 81-1/8 stock solution was introduced into the diluter and cycling began on 22 September 1981. The test was initiated two days later when ten fathead minnow with a mean (range, N=30) wet Weight and total length of 0.57(0.25-1.53) grams and A1(33-54) millimeters (Fish Weights and Lengths Log) were randomly distri- : buted to each aquarium. - I Biological observations of the fish and observations of the physical characteristics of the test solutions were made and recorded at 0, 24, 48, 72 and 96 hours. The pH, temperature and dissolved oxygen (DO) concentrations were measured in both replicates of the control, high, middle and low concentrations at 24, 48, 72 and 96 hours and in both replicates of all concentrations at 0 hour of exposure. 001015 | - . i s ' The concentrations tested and the corresponding mortality data , derived from the toxicity test were used to estimate 24-, 48-, | 72- and 96-hour median lethal concentrations (LC50) and 95% . i confidence intervals. The computer program utilized (Stephan, 1978, personal communication) estimated LCS0 values using one of ] three statistical methods in the following order of preference: moving average angle analysis, probit analysis, binomial proba- I bility. The method selected was determinbeyd the characteristics i of the data base (i.e. presence or absence of test concentrations ' causing mortality of 100% of the test animal population, test concen trations causing mortality of a partial number of animals in the t population, etc.). The computer program scanned the data base, : identified the most preferred statistical method and performed the analysis. | sess -- I. "he test material 81-1/8, was not acutely toxic to' Fathead minnows | at nominal concentrations as high as 2000 mg/% (Table 1). Al- though there appeared to be no 81-1/8 related mortality, fish exposed to2000ng/t8171/8werelethargicafter48hoursexposure. i Fishexposed to 1000, 500and250 mg/%81-1/8alsoappeared ! lethargic at 72 hours exposure. The estimated LC50 values after : 24, 48, 72 and 96 hours of exposure was >2000mg/, the highest . concentration tested. i : : 001016 6 The temperature in various `aquaria ranged from 21-22C during exposure. The pH and dissolved oxygen concentrations measured during the toxicity test are presented in Table 2. | 001017 LITERATURE CITED APHA, AWWA, WPCF. 1975. Standard methods for the examination of water and wastewater. ldth Edition, Washington, D.C. 1193 pp. \ Lemke, A.E., W.A. Brungs and B.J. Halligan. 1978. Manual for construction and operation of toxicity-tsting proportional diluters. EPA-600/3-78-072. Mount, D.I. and W.A. Brungs. 1967. A simplified dosing apparatus for fish toxicology studies. Water Res. l: 20-29. Stephan, Charles. 1978. U.S. EPA, Environmental Research Labor- atory, Duluth, Minnesota. Personal communication. 001018 Table 1. Concentrations tested, corresponding percentage : mortalities and behavioral observations of fathead minnow (Pimephales promelas) exposed to 81-1/8 under flow-through conditions. Twenty fish were exposed to each concentration. i . _-- ! conNcoemnitnraaltion Percentage m-o-rtalit (mg/2) -_-- TH Rour 48 hour 7% hes TE Four 2000 0 oad 02ibie 1000 500 250 125 control 0 0? 0? 0 0 a 0 0 0 [5d orb 1020 0 Ls - - bSuds formed on the surface of thetest solution. i Some fish were lethargic. One fish was at the surface of the test solution. sab 0? 0? 10 5 5 - 001019 Table 2. The pil and DO concentrations measured during the 96-hour flow-through toxicity test with fathead minnow (Pimephales promelas) and 8-1/8. The range of measurements made from replicate aquaria are reported. conNcoemnitnraaltion (ng/2) Ohour on 2000 7.2 1000 7.1 500 7.1 250 7.0 125 7.1 control 17.1 20 (mg/1) 2000 1000 500 250 125 control 8.38.4 ESE 8.2-8.4 Gea 8.7-8.8 9.09.1 22 a"No measurement made. 28 i 24 hour 7.2 BE 7.0 A 6.9 7.0 7.67.9 El 7.47.6 - 7.6.7.7 8.28.4 48 hour 6.8 2 6.6-6.8 6.7-6.9 6.9-7.0 7.9 2 70-81 a Ba-85 83-85 72hour 6s = 64-69 = 6.9 7.0 7.0-7.4 = sete 70-78 8.2-8.4 96 hour 68 - 67-68 6.9 7.0 6.46.9 - 5.9-6.9 - 7.8.7.9 8.2.83 SUBMITTED BY: EG&G, Bionomics Aquatic Toxicology Laboratory 790 Main Street Wareham, Massachusetts - October, 1981 | PRINCIPAL INVESTIGATOR: Brenda F. Wilson t Aquatic Biologist STUDY DIRECTOR: i DATA AUDITED BY: Gerald.A. LeBlanc, atic Toxicologist - Robert E. Bentley Director, Quality Coe Unit 001021