Document xaKBN2a39EKq5YKExZER50jG

AR226-2918 FOR DU PONT USE ONLY cc: Haskell E. I. du Pont de Nemours and Co., Inc. Laboratory for Toxicology and Industrial Elkton Road, P. 0. Box 50, Newark. Delaware 19714 Medicine nng to Haskell Laboratory Acute Toxici materials are extremely toxic by inhalation. II. PROCEDURES: A. Animal Husbandry: Young adult male Cr1:CD rats wereTeceived from Charles River Breeding Laboratories, Kingston, New York. Rats were housed singly in either 5"xll"x7" or 8"xl3"x8" suspended, steelmesh cages in rooms maintained at 50 j^ 201 relative humidity and 66-75F on a 12/12 hour light/dark cycle. Each rat was assigned a unique identification number which corresponded to a number card on the outside of the cage, and rats' tails were color-coded during exposure. Except during exposures, Purina Certified Rodent Chow* #5002 and water were available ad libitum. Rats were quarantined for 1 week prior to testing. Rats were weighed and observed twice during the quarantine period. Company Sanitized. Does nat contain TSCA CM B. Protocol: Groups of 6 rats, 8 weeks old and weighing between 234-293 grams, were restrained in perforated, stainless steel cylinders with conical nose pieces. Each group was exposed nose-ogly for_a_sjng1e. 4-hour period to an aerosol atmosphere of eHherQllHHJHBH|HH|Bljn air. Surviving rats were weighedandobserveddan^for 14'days post exposure, weekends Included when deemed necessary. C. Test Materials: Synonyms: Other Codes Stability: Submitted by Test mdterial is assumed to be stable throughout the exposure phase^ Textile Sea ford 'ers Department 2) 3% TBCU-AS: TBCU-AS Composition; Test Mixture: Contaminants: Synonyms; Other Codes: Stability: Submitted By: Chemicals & Pigments Department Jackson Laboratory ^a^jpam? a>i!Sfa@d. ^ws. na? Komsm TSCA CBi D. Generation: Liquid test material was pumped into a Spraying Systems* Nebulizer. Air Introduced at the nebulizer aerosolized the test material arid swept the aerosol stream through 3 cyclone elutrlator, The cyclone removed large particles by Inertia! impactlon, while aerodynamic particles passed through the cyclone and Into the exposure chamber. For threef^^------exposures, the air stream was humidified prior to entering the nerolizer in an attempt to increase particle size by minimizing water evaporation. E* Analytical: At approximately 30-minute intervals, calibrated volumes of chamber atmosphere were drawn through pre-weighed glass- fiber filters. Filters were dried overnight in a desclcator containing Orierite before and after sampling. Filters were weighed both wet and dry on a Cahn 26 Automatic Electrobalance*. Atmospheric concentration of partIculate was determined from the weight differential of dried filters be fore and after sampling. Particle size (mass median diameter) and percent of resplrable partlculate were determined with a Sierra Cascade Impactor during each exposure. Impactor filters were dried and weighed in the same manner as sample fitters. Chamber oxygen^as measuqgd with a Bioniarlne Model 225 Oxygen Analyzer. DurlngglBBSS^xposures, chamber temperature was measured with a thermometer, and relative humidity was measured with a Bendix* Model 556 psychrometer. III. RESULTS: A very light mist was visible In a beam of light during all exposures. Chamber oxygen levels were maintained at >_ 20.51. For 31 TBCU-AS exposures, chamber temperature ranged between 20-23C, and relative humidity ranged from 27-391. A comparison of wet and dry filter weights for both materials showed negligible weight differences. A11 reported values are based on dry weights. Company Sanitized. Does not contain JSC& rw 1. Data: Atmosphere Characterization and Mortality Particulate Concentration (mg/-r3e^a Mean S.D. Range t . Respirable Mass Median Diameter (urn) 4.3 1.8 2.0 - 6.5 27 4.2 21 - 33 >99 34 1.2 33 - 36 98 150 35 110 - 200 98 0.96 1.8 2.2 Mortality , (9 deaths/0 exposed)0 Day 0 Day 1 Totar 0/6 3/6 1/6 6/6 ParticuTate concentration represents combinec . concentrations. -- I by weight of particles with aerodynamic diameter less than 10 urn. ' Calculated for total atmosphere. Day 0 indicates during exposure. Day I indicates within I day post exposure. Particle size could not be calculated due to insufficient weight pick-up during the particle size sample. 2. Observations: During exposures: At 4.3, 27 and 34 mg/m , rats exhibited clear to red nasal discharge during exposure, an effect common to rats under restraint. Deaths occurred either during exposure or within 1 day post exposure. Surviving rats at all concentrations exhibited slight or no weight loss 1 day post exposure, followed by normal weight gain. No adverse clinical signs were seen in any rats during the post-exposure period. 'esmpsny aRiffeed. Bess not contain TCA CBF 1. Data: Atmosphere Characterization and Mortality Particulite - Cone:entration (mg/mT Mean s.b. Range 1 . Respirable" Mass Median Diameter (um) "e 12 5.5 - 40 >99 38 2.7 35 - 42 96 43 14 26 - 63 96 49, 2.6 46 - 54 >99 53 2.4 50 - 57 >99 71 11 60 - 86 >99 1.1 1.8 1.6 1.1 0.84 0.86 0 0 0 0/6 0 0 0 0/6 0 0 0 0/6 0 1 1 2/6 1 3 0 4/6 2 4 0 6/6 concentration represents combined] (Oncentrations, I ^ X -- by weigT --rf -->..g.. o-.f pf>alrt>iwcle.sJ wi -- . t-h.. a~>e- rodynamic diameter less than 10 urn. ^ Calculated fo_r^ &to_&ta_1l a.At._m__o*_sLp_--h--er--e. Day 0 indicates during exposure. Day 1 and Day 2 indicate within 1 and 2. days post exposure, respectively. Generated with water bubbler in air stream. 2. Observations: During or imnediately after exposure, some rats at all concentrations exhibited red ocular and/or nasal discharges, effects common to restrained rats. Deaths occurred during exposure or 1-2 days post exposure. Clinical signs in rats which subsequently died included labored breathing, lethargy, hunched posture, decreased muscle tone, pallor and/or brown nasal discharge. Survivors exhibited little or no weight loss and no significant clinical signs during the recovery period. ^^^^^1 SBB^cnl IV. CONCLUSIONS: An innil^ationALfc fo0n1--------------i--------^----BS--.2: 7--m|gs/fi! of solids. An ALC forj s 449nniigg/7innrooffssoelTnads. According to Haskell Laboratory Toxicity sifications, both materials are extremely toxic by inhalation. Company SanSEized. Dees not co^ain TSCA CBI Work by: Technician Supervision and Report by: Study Director: Mi Hi am J. Fasano William J. Fasano Technician -/ ~v'Laura -/. CL-^-'' r- f\( ' . A. I 1 k . -. T^JI-^-^ KinneyJ Chemist ^ a ^ ^ ^ 7~^''^^^^B^^r^u""c^^e^^/fA^^.^^pB'^d^^r^g^^e"s^"s"""'--**^"'^------ Research Toxicologist Approved by: AJO:sg1:2.23 Date Issued: July 25, 1984 " Initiated/ConinTeted: 1 iskeil LdU. Ke))url No. Number of pages in this report: 6 -^ tLi.3.^----JL k Gerald L. Kennedy ,/Jr. Section SupervisoiV Acute Investigations - 6 - ^QWpsny San56EZd. S&ss ?tui ee^ain TSCA c;@r