Document wDNrq02oD7gboORmxr54xnnK3

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may also be referredtoas PFOS, FC-95, or as a component ofFC-201 F. (I-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium salt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was 1. The testsample is FC-201 F (F-11129). CurrentinformationindicatesthatFC-201 F isa mixtureof3.0% PFOS, 36.0% diethyleneglycolbutylether,36.6% water, 8.25% acrylifcoamer,7.0% sodium octylsulfate6,.0% sulfonatedalkyl ethersodium salts,3.0% tdethanolamine,and 0.15% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities.Data may not accurately ret7ecttoxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline203. Test type: Acute static GLP: No Year Completed: 1992 Species: Pimephales promelas Supplier:ECT, SuperiorW,i, maintainedatASci-DETD, Duluth,MN. Analyticalmonitoring:Temperature,pH, DO, and conductivity Exposure period:96-hours Statisticamlethods: LC50 valuescalculateudsingtheTrimmed Spearman-Karber method. Test fishage: Juveniles(-30 days) Length and weight: Length = 0.8-1.6cm witha mean of 1.1cm Weight = 0.001-0.044g witha mean of0.013 g Loading: 0.13 g/L Pretreatment: Not noted. Test conditions: Dilutionwater: Shallow wellwater from the Two Harbors,MN area. Dilutionwater chemistry: Hardness: 155 mg/L (as CaC03) pH: 8.25 LightingC:ool-whitfeluorescelnitghtpsrovidinagdailyphotoperioodf16 hours lightand 8 hours dark. Stockand testsolutiopnreparationA:lltestsolutiownesre preparedby directadditions. Concentrations dosing rate:Once Stabilityof the testchemical solution:Described as stable. Exposure vessels: 4.5 L rectangularglasstanks (20 cm L x 15 cm W x 15 cm H) with 1 liteorftestsolution. Number of replicates:two Number of fishper replicate:10 Number of concentrations:5 plusa blankcontrol Water chemistry during the study: Conductivity range (0-96hours): 346 - 371 pmhos/cm (controlexposure) 396 - 437 pmhos/cm (1000 mg/L exposure) pH range (0-96hours): 8.24 - 8.41 (controelxposure) 7.86- 8.36 (1000 mg/L exposure) Temperature range (0-96hours): 20.7- 21.50C Dissolved oxygen range (0-96hours): 7.4- 8.6 mg/L (controelxposure) 4.6- 7.9 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Bk control,120,204, 346, 588 and 1,000 mg/L Element value: 96-hour LC50 = >1,000 mg/L 96-hour NOEC = 588 mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas described inthe Test Substance Remarks field.The value reportedappliesto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-201 F 96-hour LC50 forFathead minnow (Pimephales promelas) was determined to be >1,000 mg/L. The 96-hour No Observed Effects Concentration(NOEC) was 588 mg/L. Submifter:3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabiliKtlyi:miscrhankin3g. Dissolvoexdygenfelblelowan acceptablelevelat24 and 48 hours forthehighesttestconcentrationin one replicateT.he rangefindingtestresultdsidnotagree withthe definititvest.The range findingtestresultedin100% mortalitbyy 24 hoursat 1,000mg/L,whereas thedefinititvesthad only30% mortalitbyy 96 hours at 1,000 mg/L. Testingwas redone todetermineifthereisa relationshibpetween the surfaceareaoftheexposure chamber to the volume ofthedilutiownater/testsolution.The sample puritywas not properlycharacterizeadnd thestudylacksanalyticaclonfirmatioonfthe amount offluorochemicaplroportioinnsolution. REFERENCES This studywas conductedby ASci CorporationD,uluthEnvironmental TestingDivisionD,uluth,MN attherequestofthe3M Company, Lab Request number Kl 792, 1992. OTHER Last changed: 6/26/00 ASelCorpmuim/ASel-Duhv& EnvkoomcoW TestinDgivision AScl ReportMO 015-FHA.R3M ASelStudyID#5030415-03 STUDY TITLE ACUTE TOXICITY OF FC-201F TO FATHEAD MINNOW (PIMEPHALES PROMELAS) DATA STANDARD OECD GUIDELINE 203 STUDY COMPLETED August 24, 1992 TESTING FACILITY ASCI Corporation AScI-Duluth Environmental Testing 112 East Second Street Duluth, MN 55805 Division Tel. No. (218) 722-4040 STUDY IDENTIFICATION NUMBERS ASCI Study ID# 5030-015-03 3M Company Lab Request# K1792 Sponsor3.M Coaqmy Spms" StudyM# K1792 ASclCorpm*doWASel-Daft EnvkoomeaWTwUng DivW= ASelRcpon M# 01.0@RIA.SUM ASclft* M# 5MMI i-M 1.0GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsorfs Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Retention of Raw Data and Final Report Location of Raw Data and Final Report Acute Toxicity of FC-201F to Fathead Minnow (Pimephales promelas) OECD Guideline 203. 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106. Susan A. Beach, 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106; Tel No. (612) 778-7452. K1792. ASCI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. Jo A. Thompson Dinesh Vaishnav Donald Mount July 9-13, 1992. Dinesh Vaishnav Report Signature: Two years from the date study is completed. ASCI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, IMN 55805; Tel. No. (218) 722-4040. Sponsor3:M ConWany SponsorStudyM# K 1792 2 ASclCm"mtioutASel-Duluth Env6ran W Tenin Divitim ASclRcporItD#015-FHA.R3M ASCISb* M# 503"15-M 2.0OBJECTIVE(S) To determine the 96-h LC50 and 96-h NOEC (no observed effect concentration) of the test substance, FC-201F, for fathead minnow (Pimephales promelas) under static test conditions. This study was conducted according to the ASCI study plan retained by the Sponsor and OECD Guideline 203 (OECD 1984). 3.0 TEST METHODS Test Substance Properties: FC-201F, orange/red liquid, relative density 1.1 (water = 1), stable, and miscible with water. Test organisms L Test concentrations: 0.0 (dilution water control), 120, 204, 346, 588, and 1000 mg/L, prepared by direct additions. By 24 h, all test' solutions, except the control, were foamy. Souz*ce: Healthy, juvenile fathead minnows (Pimephales promelas) obtained from a commercial supplier. Holdinglacclimation: Held and acclimated for 15 days, during which exposed to the dilution water for 14 days. 0% mortality occurred during holding/acclimation period. At test termination, they were 0.8-1.6 cm in length and 0.001-0.044 g in weight with a mean of 0.013 g. Use in test: Did not feed 24 h before the test initiation. Used 10 organisms/replicate and 2 replicates/test concentration. Sponso3rM.CooWmy spool"sb* M# K1792 3 ASelCwpmdon/ASel-Du" EnvkmncntalTestinDgivision ASclReporItD#OMFHA.R3M ASCIStudIyD#5030-015-M Dilution Water Exposure Chambers Incubation Observations Endpoint Calculations Source: Shallow well water from the Two Harbors, Minnesota area. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, the water had a hardness of 155 mg/L (as CaC03) and a pH of 8.25. Aerated for 24 h prior to using in the test. Type: 4.5-L rectangular glass tanks, each measuring about 20 cm length X 15 width X 15 cm height. Use in test: 1 L of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Gentle aeration was provided throughout the test. Duration: 96 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool white fluorescent lamps. Temperature: 20.7-21.50C. Biological: 3 h, 6 h, and dailyorganisms with abnormal behavior stress, and dead test organisms. test organisms when observations Test and signs of Removed dead were made. Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) dailytemperature, DO, and pH, and (3) at test initiation and termination- specific conductivity. All determinations were made according to APHA methods (1989). LC50: Trimmed Spearman-Karber et. al. 1977). method (Hamilton NOEC: Dunnett's procedure (TOXSTAT statistical software, Version 3.1, University of Wyoming 1989). Sponso3rM-.Compmy SponsSotrudMy# K1792 4 ASCI CoqmnkdcWAScl-rA-bdb Envk=menW Tud% Di%isim AScl RepW M# OIS-RU.R3M ASCISb* ]D#5030-015-M 3.0PESULTS Test Substance Toxicity (Pertinent data are in Table 1) 24-h LC50: >1000 mg/L. 48-h LC50: >1000 mg/L. 72-h LC50: >1000 mg/L. 96-h LC50: >1000 mg/L. Ranges of Water Chemistry Parameters 96-h NOEC*. 588 mg/L. Total hardness (as CaCo. mgll): 155-160. Alkalinity 125-140. (as CACO, mgll): Temperatu.re (OC): 20.7-21.5. DO (mglL): 4.6-8.0. pH: 7.86-8.54. Specific conductivity (Amhoslcm): 346-437. 4.0 TEST QA/QC QA/QC Criteria Data Mortality in control exposure must not be more than 10%. Ot mortality. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. The lowest DO concentration measured was 53% of the air saturation value at 21.OOC. Test duration must be 96 h. The test duration was 96 h. Reference Substance Toxicity Final report for the most Test (thistest is optional). recent test with sodium chloride is in Raw Data I Package. Sponsor. 3M Company Spomm Study 13D#X1792 5 ASCICorponriaWASel-nukah Env4onmeaW TutingDivi"m AScl Repon M# OMNIA.IUM ASCISh* M# SW"15-M 5.0DEVIATIONS 1. The DO fell below 60% of the air saturation at 24 h and 48 h in one replicate of the highest test concentration. The dead fish were removed and aeration was adjusted. 6.0ADDITIONAL STUDY In the range-finding test, which was conducted using 1 L dilution water in i-L borosilicate glass beaker, 100% mortality occurred by 24 h at 1,000 mg/L test substance. However, in the definitive test, which was conducted as described before, only 30% mortality occurred by 96 at 1,000 mg/L test substance. As a result, the range-finding test was repeated and the data showed that by 3 h 100% mortality had occurred at 1,000 mg/L test substance. The information for the repeat test is in raw data package. 7.0 PEFEP@ENCES American Public Health Association (APHA). 1989. Standard Methods for the Examination of Water and Wastewater. APRA, Washington, D.C. Hamilton M.A., Spearman-Karber concentration 11:714-719. R.C. Russo and R.V. Thurston. method for estimating median in toxicity bioassays. Environ. 1977. Trimmed lethal Sci. Technol. organization for Economic Cooperation and Development 1981/1984. OECD Guidelines for Testing of Chemicals. Publication Information Center, Washington, D.C. (OECD). OECD University of Wyoming. 1989. Toxstat Version 3.1. Fish Physiology and Toxicology Laboratory, Department of Zoology and Physiology. University of Wyoming, Laramie,--WY. Sponsor.3M Company SponsorStudy[D# K1792 6 ASCIC*VondWWASel-Dukith FAvit@ ToodogDwwm ASCIRQMK M# 015-FHA.R3M ASCISWdyM# 503"15-03 Table 1. Acute toxicity of FC-201F to fathead minnows (P. promelas) Test substance nominal concn (mg/L) 0.0 (control) 120 204 346 58 1,000 Cumulative number of dead organisms and 3h 0 (0) 6h 0 (0) 0 (0) 0 (0) 0 (0) 1 (S) 1 (S) 0 (0) 0 (0) 0 (0) 1 S) fLl( 5) 24 h 0 (0) 0 (0) 0 (0) 0 (0) 1 (S) S (2S) 48 h 0 (0) 1 (5) 0 (0) 0 1 (5) S (2S) "Percentages are in parenthesis. mortalityo 72 h 0 (0) 1 (5) 0 (0) 0 (0) 1 (S) 6 (30) 96 h 0 (0) 2 (10) 1 (5) 1 (5) 1 (5) 6 (30) LCSO and NOEC 24-h LCSO 48-h LCSO 72-h LCSO 96-h LCSO 96-h NOBC Effect and no-effect concentrations with corresponding 95% confidence intervals Data (mg/L )b >1000 >1000 >1000 >1000 sss 9S% Confidence not calculable not calculable not calculable not calculable Not applicable interval BLCSO values were calculated based on the test substance nominal concentration. Sp--. 3M C-p-y Sponw SuWy ]D# X1792