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BACK TO MAIN SANITIZED REPORT EXPLORATORY 28-DAY ORAL TOXICITY STUDY WITH TELOMER ALCOHOL, TELOMER ACRYLATE. PFHS AND PFOS (positive control) BY DAILY GAVAGE IN THE RAT FOLLOWED BY A 14/28-DAY RECOVERY PERIOD NOTOX Project 242933 NOTOX Substances 83484, 83493, 83502, 84456 and 84141 3M Test No. T-7023, T-7024, T-7022, T-7032, T-6295 - page 1 of 451 - TELOMER ALCOHOL/TELOMER ACRYLATE/ REPORT APPROVAL PFHS/PFOS BACK TO MAIN NOTOX Project 242933 * Study Director Ir. S.C.M. de Hoog Management - Drs. W.J.A.M. Frleling Date: Q-p^'l 1 Q Q 0 - Page 2 - TELOMER ALCOHOL/TELOMER ACRYLATE/ CONTENTS PFHS/PFOS REPORT APPROVAL SUMMARY PREFACE . STUDY PLAN MATERIALS AND METHODS Test Substances Test Substance Formulation Purpose and Rationale Guidelines Archiving Test System Allocation Animal Husbandry Treatment Recovery Observations Mortality / Viability Clinical Signs Body Weights Food Consumption Water- Consumption Clinical Laboratory Investigations Haematology Clinical Biochemistry Urinalysis Pathology Necropsy Organ Weights Liver Sampling Hlstotechnology Histopathology Statistical Analysis RESULTS Observations Mortality Clinical Signs . BACK TO MAIN NOTOX Project 242933 * 2 5 7 8 9 9 10 11 11 11 12 12 13 13 13 14 14 14 14 14 14 14 15 16 17 17 17 18 18 18 19 19 20 20 20 20 - Page 3 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 RESULTS (continued) 21 Body Weight Food Consumption Clinical Laboratory Investigations Haematology Clinical Biochemistry Pathology Macroscopic Examination Organ Weights Microscopic Examination Discussion and Conclusion 21 21 22 22 23 24 24 24 25 25 . FIGURES Body Weights Body Weight Gain Food Consumption Relative Food Consumption 27 28-29 30-31 32-33 34-35 TABLES - SUMMARY DATA Clinical Signs Body Weights Body Weight Gain Food Consumption Relative Food Consumption Haematology Clinical Biochemistry Organ Weights-Organ/Body Weight Ratios Historical data Haematology / Clinical Biochemistry 36 37-60 61-64 65-68 69-72 73-76 77-88 89-100 101-112 113-118 TABLES - INDIVIDUAL DATA 119 Mortality 120-125 Clinical Signs, Daily 126-152 Body Weights 153-160 Body Weights Gain 161-168 Food Consumption Relative Food Consumption 169-172 173-184 Key to Missing Values and Remarks Haematology / Clinical Biochemistry 185 Haematology 186-221 Clinical Biochemistry 222-257 Macroscopic Findings 258-281 Organ Weights-Organ/Body Weight Ratios 282-305 APPENDIX 1 Pathology Report LAST PAGE OF REPORT 1-146 451 BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 SUMMARY Exploratory 28-day oral toxicity study with Telomer Alcohol, Telomer Acrylate, PFHS and PFOS (positive control) by daily gavage in the rat followed by a 14/28-day recovery period. Dose levels for this study, provided by the sponsor, were selected to be 0 (Vehicle Control), 3 (PFOS), 10 (PFHS) and 30 mg/kg body weight/day (Telomer Alcohol, Telomer Acrylate ).. The study was based on the following guidelines: - EEC Directive 96/54/EEC, B.7 Repeated Dose (28 days) Toxicity (oral), 1996. - OECD 407, Repeated Dose 28-day Oral Toxicity Study in Rodents, 1995 The test substance, formulated in 1% aqueous carboxymethyl cellulose, was administered dally for at least 28 days by oral gavage to SPF-bred Sprague Dawley rats. One vehicle control group and five treated groups were tested, each consisting of 8 males and 8 females. An extra 3 animals per sex per group were allowed 14 days of recovery and another 3 animals per sex per group were allowed 28 days of recovery. The following parameters were evaluated: clinical signs twice daily (treatment) or once dally (recovery); body weight twice weekly; food consumption weekly; clinical pathology after 4 weeks and at the end of both recovery periods; macroscopy at termination; organ weights and histopathology on a selection of tissues. RESULTS Telomer Alcohol: (30 mg/kg/day) 1. Total protein, globulin and total cholesterol were decreased (/) after treatment. Total protein was still decreased after 2 and 4 weeks recovery (<?). 2. Midzonal/centrilobular hypertrophy was observed in the liver, and exocrine apoptosis/single cell necrosis 1n the pancreas (</*) after treatment and had partially or completely resolved after 4 weeks recovery. Telomer Acrylate: (30 mg/kg/day) 1. Body weights were decreased at the end of treatment (?) 2. Total protein, globulin and total cholesterol were decreased (<?) and albumin/globulin ratios were increased (cf) after treatment. (30 mg/kg/day) 1. Total protein, globulin, aspartate aminotransferase activity (<?) and prothrombin time (?) were decreased and albumin/globulin ratios were Increased (rf) after treatment. Prothrombin time was still decreased after 2 weeks recovery (?). - Page 5 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 PFHS : (10 mg/kg/day) PFOS: (3 mg/kg/day) 1. Total cholesterol (d1 and ?), total-protein, globulin, triglycerides, aspartate aminotransferase activity (c?) and prothrombin time (<f and ?) were decreased; albumin/globulin ratios and alkaline phosphatase activity were increased (<?) after treatment. Prothrombin time was still decreased after 2 weeks recovery (?). 2. Liver weights were increased after treatment (<?). 3. Mldzonal/centrilobular hypertrophy was observed in the liver (cr) after treatment and had completely resolved after 4 weeks recovery. 1. Body weights and food consumption were decreased during treatment and recovery (?). 2. Total protein, globulin, total cholesterol, triglycerides, aspartate aminotransferase activity (d1) and prothrombin time (?) were decreased; albumin/globulin ratios were increased (d*) after treatment. 3. Autopsy body weights were decreased (?). Liver weights were increased after treatment and recovery (<? and ?). 4. Midzonal/centrllobular hypertrophy was observed 1n the liver (r and ?) after treatment and remained in evidence among males after recovery. From the results presented 1n this report 1t can be concluded that the positive control compound PFOS dosed at 3 mg/kg/day was indeed the compound with the most striking and persistent effects, mainly in the liver. Compounds with comparable but less severe effects as PFOS included PFHS (10 mg/kg/day) and TELOMER ALCOHOL (30 mg/kg/day). Despite slightly reduced body weights in females (TELOMER ACRYLATE only) and some slight changes in the blood after treatment, 30 mg/kg TELOMER ACRYLATE or per day did not result in clear systemic toxicity and organ dysfunction at macroscopic or microscopic level. - Page 6 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 PREFACE Sponsor Study Monitor Invoice Contact Testing Facility Study Director Histopathology 3M Toxicology Services 3M Center, Bldg 220-2E-02 St. Paul, MN 55144-1000 USA . Marvin T. Case, DVM, PhD 3M Toxicology Services 3M Center, Bldg 220-2E-02 St. Paul MN 55144-1000 Telephone (651)- 733-5180 Fax (651)- 733-1773 Barbara Nelson 3M Procurement Operations 3M Center, Bldg 224-1N-04 St. Paul, MN 55144-1000 Telephone (651)- 733-0766 Fax (651)- 733-1799 NOTOX B.V. Hambakenwetering 3 5231 DD 's-Hertogenbosch The Netherlands Ir. S.C.M. de Hoog Dr. J. Th. Wilson . . - Page 7 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 STUDY PLAN * Start of Treatment 06 October 1998 (males) 08 October 1998 (females) Start Recovery 04 November 1998 (males) 06 November 1998 (females) Blood sampling 03 November 1998 (Main groups, males) 04 November 1998 (Main and Recovery groups 1 and 2, males) 05 November 1998 (Main groups, females) 06 November 1998 (Main and Recovery groups 1 and 2, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December' 1998 (Recovery groups 2, females) Urine collection predose day 7 day 7 day 14 day 14 end of treatment end of treatment end of recovery end of recovery end of recovery end of recovery Necropsy 29-30 September 1998 (Recovery groups 1 and '2) 11-12 October 1998 (Recovery groups 1 and 2, males) 13-14 October 1998 (Recovery groups 1 and 2, females) 18-19 October 1998 (Recovery groups 1 and 2, males) 20-21 October 1998 (Recovery groups 1 and 2, females) 03-04 November 1998 (Recovery groups 1 and 2, males) 05-06 November 1998 (Recovery groups 1 and 2, females) 17-18 November 1998 (Recovery groups 1, males) 19-20 November 1998 (Recovery groups 1, females) 01-02 December 1998 (Recovery groups 2, males) 03-04 December 1998 (Recovery groups 2, females) 03 November 1998 (Main groups, males) 04 November 1998 (Main groups, males) 05 November 1998 (Main groups, females) 06 November 1998 (Main groups, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December 1998 (Recovery groups 2, females) - Page 8 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS MATERIALS AND METHODS NOTOX Project 242933 - TEST SUBSTANCES The sponsor is responsible for the completeness of all test substance data. All available information on characteristics of the test substance were taken into account before the start of the study in order to determine if they conflict with/might affect the proceeding of the study. NOTOX SUBSTANCE 83484 Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date Specific Gravity Stability in vehicle TELOMER ALCOHOL Pale to medium tan waxy solid T-7023 Not Indicated by sponsor; treated as 100% pure At room temperature in the dark Stable 07 September 2000 1.7 1% aqueous carboxymethyl cellulose: not indicated NOTOX SUBSTANCE 83493 Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date Density Stability in vehicle NOTOX SUBSTANCE 83502 TELOMER ACRYLATE Yellow liquid T-7024 Not indicated by sponsor; treated as 100% pure At room temperature protected from light Stable . 07 September 2000 Approx. 1.6 g/cnr at 50 "C 1% aqueous carboxymethyl cellulose: not Indicated Identification Description Batch Purity . Test substance storage Stability under storage conditions Expiry date Stability in vehicle White powder T-7022 Not indicated by sponsor; treated as 100% pure At room temperature in the dark Stable 07 September 2000 1% aqueous carboxymethyl cellulose: not indicated - Page 9 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 NOTOX SUBSTANCE 84456/B Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date Stability in vehicle NOTOX SUBSTANCE 84141 Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date " Stability in vehicle * PFHS Off-white crystalline solid T-7042 Not indicated by sponsor; treated as 100% pure At room temperature in the dark Stable 25 September 2000 1% aqueous carboxymethyl cellulose: not indicated PFOS White solid T-6295 Not indicated by sponsor; treated as 100% pure At room temperature in the dark Stable 07 September 2000 1% aqueous carboxymethyl cellulose: not indicated Vehicle Rationale for vehicle Stability in vehicle 1% aqueous carboxymethyl cellulose Based on trial formulations performed at NOTOX Stability over 4 hours will be determined by the sponsor TEST SUBSTANCE FORMULATION Method Formulations (w/w) were prepared daily within 4 hours prior to dosing. No adjustment was made for specific gravity of the test substance. Storage conditions At ambient temperature No chemical analysis of dose preparations was performed. Page 10 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 PURPOSE AND RATIONALE * The nature and purpose of this exploratory study was to determine and compare the relative oral toxicity in rats following oral (gastric gavage) doses administered daily over 28 days and identify developmental compounds of lesser toxicity for commercial development. This study should provide part of a rational basis for toxicological risk assessment 1n man. The oral route was selected as it 1s a possible route of human exposure during manufacture, handling or use of the test substance. GUIDELINES The protocol was reviewed and agreed by the Article 14-functionary and the Ethical Committee of NOTOX as required by the Dutch Act on Animal Experimentation. The study procedures described in this report were based on the following guidelines: 1) EC Directive 96/54/EEC, Annex IV.D, replacing EEC Directive 67/548/EEC, Part B : Methods for the Determination of Toxicity and other Health Effects; B.7: 'Repeated Dose (28 days) Toxicity (oral)*. Official Journal of the European Communities No. L248, September 1996. 2) OECD Guideline for Testing of Chemicals, Guideline 407: `Repeated Dose 28-day Oral Toxicity Study in Rodents', Paris Cedex, 27 July 1995 The protocol, study procedures and the report were not inspected by the NOTOX Quality Assurance Unit. ARCHIVING NOTOX B.V., will archive the following data for at least 10 years: protocol, raw data, report and all specimens. No data will be withdrawn without the sponsor's written consent. - Page 11 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 TEST SYSTEM * Test System Age at Start of Treatment Number of animals Randomisation Identification Sprague Dawley rat Cr1:CD BR (outbred, SPF-Quality) Recognised by international guidelines as the recommended test system (e.g. EPA, FDA, OECD, EEC). Females were nulliparous and non-pregnant. Source : Charles River, Sulzfeld, Germany Approximately 6 weeks. 84 males, 84 females At least 5 days before study start, by computer generated random algorithm according to body weight, with all animals within 20% of the sex mean. A health inspection was performed prior to commencement of treatment to ensure that the animals were in a good state of health. Earmark and tattoo ALLOCATION Group Identification Test Substance Number of animals Animal numbers males females males females 1 Main group 1 Recovery group 1 1 Recovery group 2 2 Main group 2 Recovery group 1 2 Recovery group 2 3 Main group 3 Recovery group 1 3 Recovery `group 2 4 Main group 4 Recovery group 1 4 Recovery group 2 5 Main group 5 Recovery group 1 5 Recovery group 2 6 Main group 6 Recovery group 1 6 Recovery group 2 Vehicle control 8 Vehicle control 3 Vehicle control 3 Telomer Alcohol 8 Telomer Alcohol 3 Telomer Alcohol 3 Telomer Acrylate 8 Telomer Acrylate 3 Telomer Acrylate 3 8 3 .3 PFHS 8 PFHS 3 PFHS 3 PFOS (positive control) 8 PFOS (positive control) 3 PFOS (positive control) 3 8 3 3 8 3 3 8 3 3 8 3 3 8 3 3 8 3 3 1- 8 9-11 13-15 17-24 25-27 29-31 33-40 41-43 45-47 49-56 57-59 61-63 65-72 73-75 77-79 81-88 89-91 93-95 97-104 105-107 109-111 113-120 121-123 125-127 129-136 137-139 141-143 145-152 153-155 157-159 161-168 169-171 173-175 177-184 185-187 189-191 Test substances Telomer Alcohol (substance 83484), Telomer Acrylate (substance 83493) and (substance 83502) were tested at a dose level of 30 mg/kg body weight/day. PFHS (substance 84456) was tested at a dose level of 10 mg/kg body weight/day. PFOS (substance 84141) was tested at a dose level of 3 mg/kg body weight/day. - Page 12 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 ANIMAL HUSBANDRY * Conditions Air-conditioned room with approximately 15 air changes per hour and the environment controlled with optimal conditions considered as being a temperature of 21C and a relative humidity of 50%. Lighting was 12 hours artificial fluorescent light and 12 hours dark per day. Deviations from these optimal conditions occurred, but were considered not to have affected study integrity. Accommodation Group housing of 3-4 animals per sex per cage in stainless steel suspended cages with wire mesh floors. Acclimatisation period was at least 5 days before start of treatment under laboratory conditions. Diet Free access to standard pelleted laboratory animal diet (from Carfil Quality BVBA, Oud-Turnhout, Belgium). Each batch is analysed for nutrients and contaminants are analysed on a regular basis. Results are examined and archived. Water Free access to tap-water. Certificates of analysis (performed quarterly) were examined and archived. TREATMENT Method Frequency Dose volume Dose level Oral gavage, using a stainless steel stomach tube. Formulations were placed on a magnetic stirrer during dosing. Once daily for at least 28 days, approximately the same time each day, 7 days per week. Main group animals were dosed up to the day prior to necropsy 5 ml/kg body weight. Actual dose volumes were calculated weekly according to the latest body weight. Group 1: 0 mg/kg b.w./day (Vehicle Control) Group 2: 30 mg/kg.,b.w./day (Telomer Alcohol) Group 3: 30 mg/kg b.w./day (Telomer Acrylate) Group 4: 30 mg/kg b.w./day ( ) Group 5: 10 mg/kg b.w./day (PFHS) Group 6: 3 mg/kg b.w./day (PFOS, positive control) RECOVERY Duration Recovery group 1: at least 14 days Recovery group 2: at least 28 days - Page 13 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 OBSERVATIONS . Mortality / Viability Twice.daily. Clinical signs ! During the treatment period detailed clinical observations were made twice daily in all animals: once immediately after dosing and once approximately 4 hours after dosing. During the recovery period only one observation per day was performed. The time of onset, degree and duration were recorded. All symptoms were recorded and graded according to fixed scales: Maximum grade 4: grading slight (1) to very severe (4). Maximum grade 3: grading slight (1) to severe (3). Maximum grade 1: presence is scored (1). Body weights Treatment period : on days 1, 5, 8, 12, 15, 19, 22, 25 and 28. Recovery period : on days 1, 5, 8, 12, 14, 19, 22, 25 and 28. Food consumption Weekly. . Water consumption Subjective appraisal was maintained during the study, but no quantitative investigation introduced as no effect was suspected. CLINICAL LABORATORY INVESTIGATIONS Blood samples were collected under light ether anaesthesia, between 7.30 and 9.30 a.m. immediately prior to post mortem examination for Main and Recovery group animals, and for Recovery group animals also at the end of the treatment period. The animals were fasted overnight (with a maximum of 20 hours) before blood sampling, but water was provided. Blood samples were drawn from the retro- orbital sinus of all Main and Recovery rats/sex/group and collected Into tubes prepared with EDTA for haematological parameters (0.5 ml), with citrate for clotting tests (1.0 ml) and untreated tubes for clinical biochemistry parameters (>2.0 ml). From animal numbers 17-20, 33-36, 49-52, 65-68, 81-83, 113, 115, 131 and 132 a second EDTA-sample was collected after 4 weeks, due to inexplicable clotting of the first sample. . - Page 14 -. BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 HAEMATOLOGY The following haematology parameters were determined from blood prepared with EDTA as an anti-coagulant, using the instruments listed: Parameter/Abbreviation Unit Instrumentation Erythrocyte count/RBC T/l Haemoglobin/HB mmo1/1 Haematocrit/HCT 1/1 Mean corpuscular volume/MCV fl Mean corpuscular haemoglobin/MCH fmol Mean corpuscular haemoglobin mmol/I concentrtion/MCHC Platelet count G/l Red cell distribution width/RDW % Total leucocyte count/WBC G/l Differential leucocyte count/SEG (Neutrophils) EO (Eosinophils), BASO (Basophils), LYMPH (Lymphocytes), MONO (Monocytes) l(rel) Sysmex K-1000 Sysmex K-1000 Sysmex K-1000 Sysmex K-IOOO Sysmex K-1000 Sysmex K-1000 Sysmex K-IOOO Sysmex K-1000 Sysmex K-IOOO Manual (Microscope) The following haematology parameters were determined from blood prepared with citrate as an anti-coagulant, using the instruments listed: Parameter/Abbrev1at ion Unit Instrumentation/Method Prothrombin time/PT Partial thromboplastin time/PTT sec sec Sysmex CA-5000 Sysmex CA-5000 ' - Page 15 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 CLINICAL BIOCHEMISTRY The following clinical biochemistry parameters were determined from serum samples using the instruments listed: Parameter/Abbreviai ion Unit Instrumentation Alanine aminotransferase/ (ALAT/GPT) pkat/1 Epos selective analyser 5060 (Eppendorf) Aspartate aminotransferase/ pkat/1 (ASAT/GOT) Epos selective analyser 5060 (Eppendorf) . Bilirubin, total/ BILI T. pmo1/1 Epos selective analyser 5060 (Eppendorf) Cholesterol, total/ CHOLEST. T. mmol/1 Epos selective analyser 5060 (Eppendorf) Triglycerides/ TRIGL. mmol/1 Epos selective analyser 5060 (Eppendorf) Creatinine pmo1/1 Epos selective analyser 5060 (Eppendorf) Glucose mmo1/1 Epos selective analyser 5060 (Eppendorf) Urea mmol/1 Epos selective analyser 5060 (Eppendorf) Protein, total/ PROTEIN T. 9/1 Epos selective analyser 5060 (Eppendorf) Protein, albumin/ ALBUMIN Protein, globulin/ GLOBULIN o/i 8/1 Epos selective analyser 5060 (Eppendorf) Calculation [PROTEIN T.-ALBUMIN] Albumin Globulin ratio A/G RATIO 1 Calculation [ALBUMIN/GLOBULIN] Alkaline phosphatase/ ALP pkat/1 Epos selective analyser 5060 (Eppendorf) Sodium mmo1/1 EFIX 5055 Flame Photometer Eppendorf Potassium mmo1/1 EFIX 5055 Flame Photometer Eppendorf Chloride mmo1/1 Jenway PCLM3 Calcium mmo1/1 EFIX 5055 Flame Photometer Eppendorf Phosphorus/ INORG. PHOSPH mmo1/1 ' Epos selective analyser 5060 (Eppendorf) - Page 16 BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 URINALYSIS (Recovery group animals only) * Urine samples were collected overnight (approximately 16 hrs) from all animals assigned to the Recovery groups according to the following schedule: - predose - during the treatment period on days 7, 14 and at the end of treatment - at the end of the recovery period, prior to scheduled necropsy. During the sampling period animals were deprived of food but water was provided. Urine was collected into a specimen vial, using a metabolism cage. After collection the samples were frozen and stored at -20oC at NOTOX until further analysis. PATHOLOGY NECROPSY All animals surviving to the end of the observation period were deeply anaesthetised using ether vapour and subsequently exsanguinated. All animals assigned to the study were necropsied and descriptions of all macroscopic abnormalities recorded. Prior to exsangu1nat1on a blood sample (1-2 ml) for serum collection was taken from the aorta. Serum samples were stored at -208C at NOTOX until further analysis. Inadvertently, no serum sample was collected from all Main males and females from groups 1, 2, 3 and 6 (animal numbers 97-100, 113-116, 129-132 and 177-180). Instead, reserve samples intended for clinical biochemistry might be used for possible further analysis. Samples of the following tissues and organs were collected from all animals at necropsy and fixed in neutral phosphate buffered 4% formaldehyde solution: Identification marks Adrenal glands Aorta Brain Caecum Colon Duodenum Epididymides Eyes with optic nerve and Harderian gland Female mammary gland area Heart Ileum Jejunum Kidneys Liver . Lung, infused with formalin Lymph nodes - mandibular, mesenteric Oesophagus Ovaries Pancreas . - Page 17 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 Peyer's patches (jejunum, ileum) if detectable Pituitary gland Prostate gland . Rectum Salivary glands - mandibular Sciatic nerve Seminal vesicles Spinal cord -cervical, midthoracic, lumbar Spleen Sternum with bone marrow Stomach Testes Thymus Thyroid including parathyroid Trachea Urinary bladder Uterus All gross lesions ORGAN WEIGHTS The following organ weights (and terminal body weight) were recorded from the surviving animals on the scheduled day of necropsy: Adrenal glands Brain Heart Kidneys Liver Ovaries Spleen Testes Both absolute and relative (% body weight) organ weight data are included 1n the study report. LIVER SAMPLING From all animals surviving until the scheduled necropsy at least 1 gram of the left lateral liver lobe was frozen in isopentane^. Liver samples were stored at -20'C at NOTOX and on 01 February 1999 dispatched on dry ice to the sponsor. Analysis is performed under the responsibility of the sponsor. Results may be reported by the sponsor in a separate document. HISTOTECHNOLOGY All underlined organ and tissue samples (see under Necropsy) from all Main and Recovery group animals were processed, embedded and cut at a thickness of 2-4 micrometers and stained with haematoxylin and eosin. - Page 18 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 HISTOPATHOLOGY . . Slides of all underlined organs and tissues (see under Necropsy) collected at the scheduled sacrifice from all Main group animals of all dose groups as well as from all animals of all dose groups which died spontaneously, and all gross lesions of all animals (all dose groups) were examined by a pathologist. Based on treatment related morphologic changes, the following organs were also examined from all rats of Recovery groups 1 and 2 : - liver from males of the Vehicle control, Telomer Alcohol, PFHS and PFOS groups; - liver from females of the Vehicle Control and PFOS groups; - pancreas from males of the Vehicle Control and Telomer Alcohol groups. All abnormalities were described and included in the report. STATISTICAL ANALYSIS The following statistical methods were used to analyse the data: Univariate one-way analysis of variance was used to assess the significance of intergroup differences. . If the variables could be assumed to follow a normal distribution, the Dunnett test (many-to-one t-test) based on a pooled variance estimate was applied for the comparison of the treated groups and the control groups for each sex. The Steel-test (many-to-one rank test) was applied instead of the Dunnett-test when the data could not be assumed to follow a normal distribution. All tests were two-sided and in all cases p < 0.05 was accepted as the lowest level of significance. Group means were calculated for continuous data and medians were calculated for discrete data (scores) in the summary tables. Test statistics were calculated on the basis of exact values for means and pooled variances. Individual values, means and standard deviations may have been rounded off before printing. Therefore, two groups may display the same printed means for a given parameter, yet display different test statistics values. References: - C.W. Dunnett . A Multiple Comparison Procedure for Comparing Several Treatments with a Control, J. Amer. Stat. Assoc. 50, 1096-1121 (1955). - R.G. Miller Simultaneous Statistical Inference, Springer Verlag, New York (1981). - Page 19 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ RESULTS PFHS/PFOS NOTOX Project 242933 - OBSERVATIONS MORTALITY . No treatment-related mortality occurred during the study period. One female receiving TELOMER ACRYLATE died following blood sampling, prior to necropsy. This was considered to be an accidental death. CLINICAL SIGNS TELOMER ALCOHOL Rales were noted in one male immediately and 4 hours after dosing. This was observed at the end of the treatment period until the third week of recovery. TELOMER ACRYLATE A hunched posture was observed in two Main group females in weeks 2 and 3 of the treatment period and in two Recovery group females at the end of treatment and during the first week(s) of recovery. Piloerection was also observed in the one female during the first week of recovery. In general the findings were observed immediately and 4 hours after dosing. A hunched posture was observed immediately and 4 hours after dosing in one Main group female from week 3 until the end of the treatment period. PFHS A hunched posture was observed immediately and 4 hours after dosing in one male at the end of treatment until the second day of recovery. A similar finding was seen 4 hours after dosing in one Main group female on one occasion at the end of treatment. PFOS A hunched posture was observed in two Main group females in weeks 3 and 4 of treatment. A swelling on the abdominal skin was observed in one Main group male at the end of the treatment period. Other findings such as alopecia, salivation, a missing tail apex, red discolouration of the skin, wounds, scabs, scales, fissures with bleeding on the paws, swelling of the ear and eye abnormalities were noted among the treated and/or control animals. These findings were considered to be of no toxicological significance as incidences remained within the range of biological variation for rats of this age and strain or could be attributed to the blood sampling procedure. - Page 20 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 BODY WEIGHT . TELOMER ALCOHOL Body weight gain of males was decreased at the end of the recovery period when compared to the corresponding control values. Absolute body weights of these animals remained however within the range of control values. TELOMER ACRYLATE . Body weights of females were slightly decreased when compared to control values at the end of the treatment period. PFHS Body weight gain of males was decreased at the end of the recovery period. Absolute body weights of these males were also below control values, but did not differ with statistical significance. PFOS . Body weights and body weight gain of females were decreased with statistical significance from week 2 of treatment until the end of the recovery period. Statistical significance was achieved in the majority of cases, mainly for absolute body weights. Among males, body weight gain was reduced with statistical significance in weeks 3 and 4 of the recovery period. Absolute body weights of these males were also below control values, but did not differ with statistical significance. Body weights and body weight gain of animals receiving remained in the same range as controls over the treatment and recovery period. FOOD CONSUMPTION PFHS Absolute food consumption among males was slightly reduced at the end of the 4week recovery period. However, after correction for body weight this effect was not observed anymore. PFOS Absolute food consumption of females was reduced from week 3 of treatment until the end of recovery. This effect was less apparent after allowance for body weight. . Among males, absolute food consumption was slightly reduced at the end of the 4- week recovery period, but relative food consumption was within control values. There were no differences in food consumption before or after allowance for body weight between animals receiving TELOMER ALCOHOL, TELOMER ACRYLATE or and control animals. - Page 21 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 CLINICAL LABORATORY INVESTIGATIONS HAEMATOLOGY TELOMER ALCOHOL After 4 weeks, red blood cell count, haemoglobin and haematocrit values were decreased 1n females. After 2 weeks recovery, prothrombin time was decreased in females. After 4 weeks recovery, red cell distribution width was decreased in males. TELOMER ACRYLATE After 4 weeks, total red blood cell count was slightly decreased in males. This decrease was no apparent anymore after the 2- and 4-week drug-free recovery periods. After 4 weeks, differential leucocyte count revealed decreased relative values of neutrophilic granulocytes and increased relative lymphocytes values in males. Partial thromboplastin time was increased in males of this dose group. Prothrombin time was decreased in females. After 2 weeks recovery, prothrombin time was still decreased in females. After 4 weeks recovery, red cell distribution width was decreased in males. PFHS After 4 weeks, total red blood cell count, haemoglobin, haematocrit, total number of platelets and prothrombin time were decreased in males. Total red blood cell count, haematocrit and prothrombin time were decreased in females. After 2 weeks recovery, prothrombin time and total platelet numbers were decreased 1n females. After 4 weeks recovery, no statistically significant differences when compared to controls were observed. PFOS After 4 weeks, mean corpuscular haemoglobin was increased in males and haematocrit and prothrombin time were decreased in females. After the 2- and 4-week drug-free recovery periods no statistically significant differences were observed. - Page 22 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 CLINICAL BIOCHEMISTRY . TELOMER ALCOHOL ' After 4 weeks, total cholesterol, total protein and globulin values were decreased in males. After 2 weeks recovery, total protein was still decreased 1n males. After 4 weeks recovery, total cholesterol, total protein, potassium and calcium values were decreased in males. . TELOMER ACRYLATE After 4 weeks, total cholesterol, total protein and globulin were decreased and albumin/globulin ratios were increased in males. After 2 weeks recovery these differences were not apparent anymore. After 4 weeks recovery, total protein, potassium and calcium values were decreased in males. After 4 weeks, aspartate aminotransferase activity, total protein, globulin and potassium values were decreased and albumin/globulin ratios were increased in males. After 2 weeks recovery, glucose values were increased and sodium values were decreased in males. Urea was increased in females. After 4 weeks recovery, total protein, inorganic phosphate, potassium and calcium values were decreased in males. PFHS After 4 weeks, aspartate aminotransferase activity, total cholesterol, triglycerides, total protein and globulin values were decreased and albumin/globulin ratios, alkaline phosphatase activity and chloride were increased in males. Total cholesterol was decreased and chloride values were increased in females. After 2 weeks recovery, no statistically significant differences were observed anymore. After 4 weeks recovery, total cholesterol, glucose and potassium values in males were below control values with statistical significance. pfos . After 4 weeks, aspartate aminotransferase activity, total cholesterol, triglycerides, total protein and globulin values were decreased and albumin/globulin ratios and chloride were Increased in males. Urea and chloride values were increased in females. After 2 weeks recovery no such findings were observed. After 4 weeks recovery, aspartate aminotransferase activity, total bilirubin, total-protein, potassium and' calcium values were decreased and chloride was increased in males. - Page 23 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 PATHOLOGY ' . MACROSCOPIC EXAMINATION - Macroscopic observations at necropsy did not reveal any alterations that were considered to have arisen as a result of treatment. The few macroscopic findings recorded at all sacrifices were unremarkable and did not distinguish treated rats from the vehicle control group. ORGAN WEIGHTS PFHS After 4 weeks, liver weights and Hver:body weight ratios were increased in males. After 2 and 4 weeks recovery, this effect had disappeared. Increased brain:body weight and testes:body weight ratios observed after the 4- week recovery period is attributed to the slightly low autopsy body weights of these animals. PFOS After 4 weeks, autopsy body weights were decreased 1n females. Liver weights and liver:body weight ratios were increased in males and females. Kidney:body weight ratios were increased in females. After 2 weeks recovery, liver weights and liver:body weight ratios were still increased in males. After 4 weeks recovery, liver:body weight ratios were increased in males and females and kidney:body weight ratios were increased 1n females. Other changes noted 1n PFOS treated animals included decreased absolute heart weights and increased brain:body weight ratios in females after 4 weeks of treatment and 2 weeks recovery. In addition, absolute spleen weights were increased in males after 2 weeks recovery and testes:body weight ratios were increased after 4 weeks recovery. All these changes were likely to reflect slightly low or high body weights at autopsy. Organ weights and organ:body weight ratios of animals treated with TELOMER ALCOHOL, TELOMER ACRYLATE or were considered to be similar to those of control animals. - Page 24 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 MICROSCOPIC EXAMINATION (see Appendix 1) . TELOMER ALCOHOL After 4 weeks, midzonal/centrllobular hypertrophy was seen at a minimal or slight degree in six males. In the pancreas a slight increase in the incidence of minimal or slight degrees of exocrine apoptosis/single cell necrosis was recorded in six males. After 2 and 4 weeks recovery, the liver changes had partially or completely resolved and the pancreas findings returned to the level of the vehicle control group. PFHS After 4 weeks, midzonal/centrilobular hypertrophy was seen at slight to moderate degrees in all males. After 2 weeks recovery, this finding had partially resolved. After 4 weeks recovery, this finding had completely resolved. . PFOS After 4 weeks, midzonal/centrllobular hypertrophy was seen at minimal to moderate degrees in all males and females. After 2 and 4 weeks recovery, this finding had partially or completely resolved in females, however remained in evidence in males. The remainder of the microscopic findings recorded after treatment and recovery were within the range of background pathology encountered in Sprague Dawley rats of this age ad strain and occurred at similar incidences and severity in both control and treated rats. DISCUSSION AND CONCLUSION Following daily oral administrations of TELOMER ALCOHOL, TELOMER ACRYLATE, PFHS or PFOS to male and female Sprague Dawley rats for at least 28 days, treatment-related changes were most pronounced in the TELOMER ALCOHOL, PFHS and PFOS groups. Hepatocellular hypertrophy 1n the liver together with higher liver weights was seen in animals receiving PFHS and PFOS. In the TELOMER ALCOHOL group the hepatic hypertrophy was not accompanied by increased liver weights. This liver enlargement is most likely related to the higher enzymatic activity in metabolising a xenobiotic compound. Among males receiving PFOS this effect was rather persistent since it was still observed at the end of the 4-week drugfree period. Minimal to slight exocrine apoptosis/single cell necrosis was recorded in the pancreas of males receiving TELOMER ALCOHOL for 4 weeks and had resolved after the recovery period. - Page 25 - BACK TO MAIN TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS NOTOX Project 242933 Investigations of the blood showed that various parameters were slightly reduced in one or both sexes after treatment. The most characteristic decreases were: total protein and globulin in all dose groups; - total cholesterol in all dose groups except for the group; * triglycerides in the PFHS and PFOS groups; - aspartate aminotransferase activity and prothrombin time in the , PFHS and PFOS groups. In the majority of cases these changes were not observed anymore after a 2-week drug-free period. A possible explanation for these changes could be liver damage as the liver is involved in the synthesis and metabolism of these compounds. However, the only microscopic change noted 1n the liver was hepatocellular hypertrophy in the TELOMER ALCOHOL, PFHS and PFOS groups only. Moreover, it should be noted that the majority of values remained within the range of values that can be expected for animals of this age and strain. Changes in clinical biochemistry parameters noted after 4 weeks of recovery but not after 2 weeks were considered to have occurred by chance. At this time point in the study it seems very unlikely that they represent an effect of treatment. Fluctuations in electrolyte levels were seen at all time points in all dose groups. Moreover, urea levels and kidney weights were increased after treatment in females receiving PFOS. However, in the absence of evidence of renal dysfunction at microscopic level these changes are considered to be of little toxicological relevance. Haematological examination revealed slightly decreased red blood cell counts, haemoglobin and/or haematocrit values in animals treated with TELOMER ALCOHOL, TELOMER ACRYLATE, PFHS and PFOS. However, since the effects were slight in nature and no further evidence of an anaemic response was obtained at macroscopic or microscopic level, the toxicological relevance of these findings is doubted. Other haematological changes noted were not supported by corresponding signs of toxicity and again the toxicological impact is considered to be of little relevance also because the values were within the range of historical data. An effect on body weight and food consumption was most pronounced among females receiving PFOS. This effect lasted until the end of the recovery period. Body weights of females receiving TELOMER ACRYLATE were slightly reduced at the end of treatment. Other effects on body weight gain and food consumption were mainly seen at the end of the recovery period and therefore no toxicological relevance is attached to these findings. Clinical signs such as a hunched posture, piloerection and/or rales were observed incidentally in all five dose groups in a few animals only. At this low incidence and the severity observed these symptoms are considered not to represent a clear indication of toxicity. From the results presented in this report it can be concluded that the positive control compound PFOS dosed at 3 mg/kg/day was indeed the compound with the most striking and persistent effects, mainly in the Uver. Compounds with comparable but less severe effects as PFOS included PFHS (10 mg/kg/day) and TELOMER ALCOHOL (30 mg/kg/day). Despite slightly reduced body weights in females (TELOMER ACRYLATE only) and some slight changes in the blood after treatment, 30 mg/kg TELOMER ACRYLATE or per day did not result in clear systemic toxicity and organ dysfunction at macroscopic or microscopic level. - Page 26 - TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS BACK TO MAIN NOTOX Project 242933 FIGURES 27 NOTOX Project 242933 BODY WEIGHTS MALES GRAM BACK TO MAIN BW-SPLT - 1 08-DEC-98 - GROUP 1 (VEHICLE CONTROL) - GROUP 2 (TELOMERALCOHOL) - GROUP 3 (TELOMERACRYLATE) - GROUP 4 - GROUP 5 (PFHS) - GROUP (PFOS) 28 NOTOX Project 242933 BODY WEIGHTS FEMALES GRAM BACK TO MAIN BW-SPLT - 2 08-DEC-98 -------------- GROUP 1 (VEHICLE CONTROL) ............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 ------------- GROUP 5 (PFHS) -------------- GROUP 6 (PFOS) 29 NOTOX Project 242933 BODY WEIGHT GAIN MALES X BACK TO MAIN BWG-SPLT - 1 08-DEC-98 -------------- GROUP 1 (VEHICLE CONTROL) .............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 ------------- GROUP 5 (PFHS) -------------- GROUP 6 (PFOS) 30 NOTOX Project 242933 BODY WEIGHT GAIN FEMALES BACK TO MAIN BWG-SPLT - 2 08-DEC-98 -------------- GROUP 1 (VEHICLE CONTROL) .............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 ------------- GROUP 5 (PFHS) -------------- GROUP 6 (PFOS) 31 NOTOX Project 242933 FOOD CONSUMPTION MALES G/ANIMAL/DAY BACK TO MAIN FC-SPLT - 1 01-MAR-99 ----------:---- GROUP 1 (VEHICLE CONTROL) .............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 ------------- GROUP 5 (PFHS) -------------- GROUP 6 (PFOS) NOTOX Project 242933 FOOD CONSUMPTION FEMALES G/ANIMAL/DAY BACK TO MAIN FC-SPLT - 2 01-MAR-99 -------------- GROUP 1 (VEHICLE CONTROL) ............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) --------------- GROUP 4 ------------- GROUP 5 (PFHS) -------------- GROUP 6 (PFOS) 33 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION MALES G/KG BODY VEIGHT/DAY BACK TO MAIN RFC-SPLT - 1 01-MAR-99 -------------- GROUP 1 (VEHICLE CONTROL) .............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 ------------- GROUP 5 (PFHS) -------------- GROUP 6 (PFOS) 34 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION FEMALES G/KG BODY VEIGHT/DAY BACK TO MAIN RFC-SPLT - 2 01-MAR-99 -------------- GROUP 1 (VEHICLE CONTROL) .............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 -------- GROUP 5 (PFHS) -------------- GROUP 6 (PFOS) TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS BACK TO MAIN NOTOX 242933 TABLES - SUMMARY DATA 36 BACK TO MAIN NOTOX Project 242933 SYM-SUM - 1 1 7 - M A R - 99 CLINICAL SIGNS (SUMMARY) MALES GROUP 1 (VEHICLE CONTROL) * SIGN (MAX.GRADE) TREATMENT WEEKS: 1........... 2 ............3 ............4............ 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB SKIN / FOR / PLUMAGE ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (CHEST) . ALOPECIA (3) (FORELEG LEFT) ALOPECIA (3) (HINDLEG LEFT) ALOPECIA (3) (HINDLEG RIGHT) SCABS (3) (NECK) VARIOUS BULGING EVE (1) (EYE RIGHT) . DARK (3) (EYE RIGHT) G: ........... 1111111111........... %: ........... 1111111111........... G: ..... 1111111111111111111........ %: ..... 1111111111111111111........ G: 111111111111111111111111111111111111.. %: 111111111111111111111111111111111111.. G: ..11............................. . %: ..11....................................... G: . . . 1222222222221111111111111111111111111. . . %: . . . 1111111111111111111111111111111111111. . . G: .....111111111111133illuminili..... %: ....11111111111111111111111111111...... G: ............ 1111111111111111111111111111111111. . . %: ............ 1111111111111111111111111111111111. . . G; ....................................111111111111111111111111111 %: ....................................111111111111111111111111111 G: %: G: %: G: Median value of the highest individual grades % : Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.... A = more than 95%) Time after treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 1 (VEHICLE CONTROL) SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEST) VARIOUS BULGING EYE (1) (EYE RIGHT) DARK (3) (EYE RIGHT) G: .....12222222 %: ..... 22222222 G: 11 %: 12 G: 11 %: 12 BACK TO MAIN SYM-SUM - i 2 5 - M A R - 99 G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) 38 BACK TO MAIN NOTOX Project 242933 SYM-SUM - 3 1 7 - M A R - 99 CLINICAL SIGNS (SUMMARY) MALES GROUP 2 (TELOMER ALCOHOL) SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 ............ 3 .............4 .............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB BREATHING RALES (3) SKIN / FOR / PLUMAGE ALOPECIA (3) (CHEEK RIGHT) ALOPECIA (3) (NECK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (SHOULDER LEFT) ALOPECIA (3) (FORELEG LEFT) SCABS (3) (SNOUT) SCABS (3) (BACK) SCABS (3) (NECK) WOUND (3) (GENITAL REGION) RED STAINING (3) (PERIORBITAL REGION LEFT) RED STAINING (3) (PERIORBITAL REGION RIGHT) G: ....................................................... Ill %: ....................................................... Ill G: ............................... 111111111...... %: ............................... 111111111...... G: .......................................... 11111 %: .......................................... 11111 G : ........... 1111111111111111111111111111111111111111111111 %: ........ 1111111111111111111111111111111111111111111111 ...... .................................. 11 %: .... ................................. 11 G: 1111111 %: 1111111 G: ..... ....................................................1 %: .......... ...............................................1 G: 1 %: 1 .................................. ...... 1111 %: .......................................... 11......1111 G: 1 %: 1 G: %: G: %: G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%, . Time after treatment (H.Mi) A: 0.00 B: 4.00 A = more than 95%) NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 2 (TELOMER ALCOHOL) SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. BREATHING RALES (3) SK IN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) SCABS (3) (SNOUT) RED STAINING (3) (PERIORBITAL REGION LEFT) RED STAINING (3) (PERIORBITAL REGION RIGHT) G: 1111111111111111..1111 % : 1222222222222223..3333 G: 11111 %: 12222 G: 11... %: 12... G: ...... Ill %: ...... 222 G: .... 1 --- %: ... 2___ BACK TO MAIN SYM-SUM - 4 25-MAR-99 G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.-.., A = more than 95%) 40 BACK TO MAIN NOTOX Project 242933 SYM-SUM - o 17-MAR-99 CLINICAL SIGNS (SUMMARY) MALES GROUP 3 (TELOMER ACRYLATE) * SIGN (MAX.GRADE) TREAUffiNT WEEKS: 1............ 2 .............3 ............ 4 .............5. DAYS: 1.2.3.4.5.6.7.1.2-3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB SKIN / FOR / PLUMAGE ALOPECIA (3) (NECK) . ALOPECIA (3) (FLANK LEFT) ALOPECIA (3) (CHEST) ALOPECIA (3) (FORELEG RIGHT) SCABS (3) (BACK) SCABS (3) (TAIL) RED STAINING (3) (NECK) G: %: .......................;...................................m11i1l11l1.. G: 11112221111111111111................ %: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ................ G: 111222222211111111111111111111111111. %: lllllllllllllllllllllllllllimillll. G: 11122222221111111111................ %: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ................ G: ..................................... %: ..................................... G: ..................................... %: ..................................... G: .................................... %: ..................................... , . 1 1 1 1 .......... , . 1 1 1 1 .......... ..1 1 1 1 1 1 1 1 1 1 1 1 1 .. ,.1 1 1 1 1 1 1 1 1 1 1 1 1 .. G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., Time after treatment (H.Mi) A: 0.00 B: 4.00 41 A = more than 95%) NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 3 (TELOMER ACRYLATE) SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. SKIN / FOR / PLOMAGE RED STAINING (3) (NECK) G: ...................... 111111. %: ...................... 333333. BACK TO MAIN 'S Y M - S O M - 6 25-MAR-9 9 G: Median, value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) BACK TO MAIN NOTOX Project 242933 SYM-SUM - 7 1 7 - M A R - 99 CLINICAL SIGNS (SUMMARY) MALES GROUP 4 SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ... ......... 2 ............ 3 ............ 4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB SKIN / FOR / PLUMAGE ALOPECIA (3) (EYE RIGHT) ALOPECIA (3) (FORELEG RIGHT) SCABS (3) (NECK) RED STAINING (3) (PERIORBITAL REGION LEFT) VARIOUS EYE RIGHT, INJURED (1) DEHYDRATED (3) (EYE RIGHT) BULGING EYE (1) (EYE RIGHT) DARK (3) (EYE RIGHT) G: %: G: ..................................................... 11... %: ..................................................... 11... G: ................................ 111111111111 %: ................................ 111111111111 G: .................... 11111 %: .................... 11111 G: %: G: %: G: %: G: %: G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) Time after treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 4 SIGN (MAX.GRADE) LOCATION , RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... S. SKIN / FOR / PLUMAGE ALOPECIA (3) (EYE RIGHT) VARIOUS EYE RIGHT, INJURED (1) DEHYDRATED (3) (EYE RIGHT) BULGING EYE (1) (EYE RIGHT) DARK (3) (EYE RIGHT) G: ........... 11U11U11111111. % ............. 2223333333333333. G: 1111111111111111111111111111. %: 1222222222222223333333333333. G: .22222....................... %: .22222....................... G: 111111 %: 122222 G: 111111 %: 232222 BACK TO MAIN SYM-SUM - S 2 5 - M A R - 99 G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%, ..., A = more than 95%) BACK TO MAIN NOTOX Project 242933 SYM-SUM - 5 1 7 - M AX-99 CLINICAL SIGNS (SUMMARY) MALES GROUP 5 (PFHS) * SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 .............3 ............ 4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.S.7.1.2.3.4.5.S.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABAHABAEABAEAB POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE WOUND (3) (BACK) RED STAINING (3) (EAR LEFT) RED STAINING (3) (SNOUT) RED STAINING (3) (NECK) RED STAINING (3) (CERVICAL REGION) VARIOUS DARK (3) (EYE RIGHT) G: ...................................................... 1111 %: ......... :................................. 1111 G: ............................ Ill %: ............................ Ill ........... 11111111111 %: ........ 11111111111 G: .................................................... 11 --%: .....................................................11 --G: %: G: %: G: %: G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.. Time after treatment (H.Mi) A: 0.00 B: 4.00 A = more than 95%) NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 5 (PFHS) SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE RED STAINING (3) (NECK) RED STAINING (3) (CERVICAL REGION) VARIOUS DARK (3) (EYE RIGHT) G: 11 %: 12 ......... 11.................... %: ...... 22.................... G: ........ 1111111111111111111. %: .........2222223333333333333. G: 11 %: 12 BACK TO MAIN SYM-SUM -tO 25-MAR-9 9 G: Median value of the highest individual daily grades %: Petcent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.... A = more than 95%) BACK TO MAIN NOTOX Proiect 242933 SYM-SUM -11 1 7 - M A R - 99 CLINICAL SIGNS (SUMMARY) MALES GROUP 6 (PFOS) SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 ............ 3 ............ 4 ............ 5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABAEABABABAB SKIN / FOR / PLUMAGE SHELLING (3) (ABDOMEN) ALOPECIA (3) (CHEEK LEFT) SCABS (3) (NECK) RED STAINING (3) (HEAD) G: .................................................. 111111.. % ............. ...................................... min.. ............. '........ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ..... '............ % ........................... 1 1 1 1 1 1 1 1 1 1 1 1 1 1 .................. g : .................................m i n i .................. %: .................................1 1 1 1 1 1 1 .................. G: .......................................................... .%: ................ ................................. G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% arid 1 5 % , . A = more than 95%) Time dfter treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) MALES . GROUP 6 (PFOS) SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ..... 2 ..... 3 ___ ...4......5. SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) ......................111111111. %: .......... ........333333333. BACK TO MAIN S Y M - S U M -12. 2 5 - M A R - 99 G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., 48 A = more than 95%) BACK TO MAIN NOTOX Project 242933 S Y M - S U M - 13 1 7 - M A R - 99 CLINICAL SIGNS (SUMMARY) FEMALES . GROUP 1 (VEHICLE CONTROL) . SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 ............ 3 ............ 4 .............5. DAYS: 1.2.3.4.5.5.7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB SKIN / FOR / PLUMAGE ALOPECIA (3) (GENERAL) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (FLANK LEFT) ALOPECIA (3) (FLANK RIGHT) ALOPECIA (3) (SHOULDER LEFT) ALOPECIA (3) (THIGH HIND LEFT) ALOPECIA (3) (THIGH HIND RIGHT) ALOPECIA (3) (HINDLEG LEFT) SCABS (3) (ABDOMEN) WOUND (3) (ABDOMEN) SECRETION / EXCRETION SALIVATION (3) VARIOUS MISSING (1) (TAIL APEX) G: %: G: %: G: ...............................111111111 %: ................. -............ 111111111 G: %: G: 1111222211111111111111111111111111111111111111111111111111 % : 1111111111111111111111111111111111111111111111111111111111 G: ....................................... 111111111111111111 %: ......................... ..............1111111111111111111 G: ........... 111111111111111.1111111111111111111111111111111 %: ........... 111111111111111.1111111111111111111111111111111 G: ............................. 1111111111111111111111 %: ....... ..................... 1111111111111111111111 G: ...11111111111........................... 111111111. %: ...11111111111........................... 111111111. G: 11111111111111....................1111111111111111..... Ill %: 11111111111111.................... 1111111111111111.... Ill G: ............... ..................... 111111111111111 %: .................................... 111111111111111 G: ................................................... 1111111 %: ............................................. ......1111111 ..................................................... 1 ....... % .................................................... 1...... G: 1 %: 1 G: ......................... 1111111111111111111111111111111.. %: ......................... 1111111111111111111111111111111.. G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.... A = more than 95%) Time After treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Project 242933 CLINICAL SIGNS, DAILY (SUMMARY) FEMALES . GROUP 1 (VEHICLE CONTROL) SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ...... 2 ..... 3 ..... 4..... 5. SKIN / FUR / PLUMAGE ALOPECIA (3) (GENERAL) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (FLANK LEFT) ALOPECIA (3) (FLANK RIGHT) ALOPECIA (3) (THIGH HIND LEFT) ALOPECIA (3) (THIGH HIND RIGHT) SCABS (3) (ABDOMEN) G: ................ 11111111111 %: ................ 33333333333 G: ............ ..11.......... %: .............. 33.......... ....... 11111111111........ %: ..... 22222222233.......... G: ......... 1111.............. %: ......... 2222............... .........1 1 1 1 1 1 1 1 1 1 1 .......... %: ..... 22222222233....... . G: 11111111111111111 %: 12222222222222233 G: 11122222222222222 %: 12222222333322233, G: ...111111111111.., %: ...222222222222... G: 111111111111111 %: 122222222222222 G: ...1111111111.. %: ...2222222222.. BACK TO MAIN S Y M - S U M -l-g 25-MAR-99 G: Median value of the highest individual daily grades % : Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%.... A = more than 95%) 50 BACK TO MAIN T-l0\ NOTOX Project 242933 SYM-SUM 17-MAR- CLINICAL SIGNS (SUMMARY) FEMALES . GROUP 2 (TELOMER ALCOHOL) * SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 ............ 3 .............4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB SKIN / FUR / PLUMAGE ALOPECIA (3) (HEAD) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (CHEEK RIGHT) ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) ALOPECIA (3) (FORELEGS) SCALES (3) (CHEEK LEFT) SCALES (3) (CHEEK RIGHT) SCABS (3) (FLANK LEFT) RED STAINING (3) (EAR RIGHT) G: %: G: ......... '............................. 1111111111........ %: ....................................... 1111111111........ G: 11 % ........................................................... 11 G: 11111 %: ...................................................... 11111 G: 11111111111111111....... 1 %: 11111111111111111....... 1 G: ...............................................1111111112.. %: ..................... ....................... ..1111111111.. G: %: G: %: G: .................................. 11 %: .................................. 11 G ............. 11 %: ........... 11 G: Median value of the highest individual grades % : Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) Time ^fter treatment (H.Mi) A: 0.00 B: 4.00 51 NOTOX Proiect 242933 CLINICAL SIGNS, DAILY. (SUMMARY) FEMALES GROUP 2 (TELOMER ALCOHOL) SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. SKIN / FUR / PLUMAGE ALOPECIA (3) (HEAD) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (CHEEK RIGHT) SCALES (3) (CHEEK LEFT) SCALES (3) (CHEEK RIGHT) G: ...... 2222211 %: G: .1112222222222 %: .2222333333333 G: ___ 1222222222 %: ___ 3333333333 G: ...... 1 1 1 1 1 1 1 %: ...... 2222222 G: ...... 1 1 1 1 1 1 1 %.: BACK TO MAIN SYM-SUM -*6 2 5 - M A R - 99 G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) BACK TO MAIN NOTOX Proiect 242933 SYM-SUM - 17 1 7 - M A R - 99 CLINICAL SIGNS (SUMMARY) FEMALES GROUP 3 (TELOMER ACRYLATE) ; . g-frai (MAX.GRADE) TREATMENT ' WEEKS: 1 ............ 2 ............ 3 ............ 4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE PILOERECTION (1) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (CHEST) ALOPECIA (3) (SHOULDER LEFT) FISSURES WITH BLEEDINGS (3) (PAW HIND RIGHT) SCABS (3) (BACK) SCABS (3) (NECK) RED STAINING (3) (HEAD) RED STAINING (3) (BACK) RED STAINING (3) (NECK) RED STAINING (3) (PAW HIND RIGHT) G: .................. ...1111111111111................ 1111111 %: ......... :....... 1111111111111............ 1111111 G: %: G: 1111222211111111111111111111112122222211111111111111111111 %: 1111111111112222111111111133321111111122222232222222211111 G: ......................... 11111111111 %: ......................... 11111111111 G: ....................... 1111111...... %: ................ .......1111111..... G: .......................................11............... %: ....................................... 11................. G: 11 %: 11 G: 111111 %: 111111 G: 111............... 1111111 %: 111............... 1111111 G: 111111 %: 111111 G: 1111111111111........ 1111 %: 1111111111111......... 1111 G: 111111111111111 %: 111111111111111 G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., Time After treatment (H.Mi) A: 0.00 B: 4.00 53 A = more than 95%) N O T O X Pro-iect 242933 CLINICAL SIGNS, DAILY (SUMMARY) FEMALES GROUP 3 (TELOMER ACRYLATE) SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE PILOERECTIQN (1) ALOPECIA (3) (ABDOMEN) RED STAINING (3) (HEAD) G: 11111111111111............. %: 23333332222222............. G: .1111...................... %: .2222...................... G: 11111111111111............. %: 12222222222222............. G: ..........................11 % ............................77 BACK TO MAIN S Y M - S U M -IS 2 5 - M A R - 99 * ' G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,... A = more than 95%) BACK TO MAIN NOTOX Proiect 242933 SYM-SUM - 19 1 7 - M A R - 99 CLINICAL SIGNS (SUMMARY) FEMALES GROUP 4 . SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 ............ 3 ............ 4 ............ 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1. TIME: ABABABABABABABABABABABA8ABABABABABABABABABABABAHABAEABABA3 POSTURE HUNCHED POSTURE (1) G: %: 1111111111111111111111111111 1111111111111111111111111111 SKIN / FUR / PLUMAGE SWELLING (3) (EAR RIGHT) ...............1111111111111 %: ........... 1111111111111 ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) G: ................. 111111111111... 1111111111111.... 1111111.. % : ................. 111111111111... 1111111111111___ 1111111.. G: 111111111111111111111111111111111111 %: 111111111111111111111111111122222222 ALOPECIA (3) (THROAT REGION) G: %: 222222222222222222222222222222221111 111111111111111111111111111111111111 SCALES (3) (THROAT REGION) G: ....................... 1111111111111 %: ....................... 1111111111111 SCABS (3) (NECK) G: %: RED STAINING (3) (EVE RIGHT) ' G: %: RED STAINING (3) (PERIORBITAL REGION RIGHT) G: %: RED STAINING (3) (EAR LEFT) G: ...................... 111111111 %: ...................... 111111111 RED STAINING (3) (EAR RIGHT) G: ........... 1111111111111111111111111111111111111111111111 % : ............1111111111111111111111111111111111112211111111 SECRETION / EXCRETION WATERY DISCHARGE FROM EYE (3) G: (EYE RIGHT) . %: G: Median value of the highest individual grades .- %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) Time after treatment (H.Mi) A: 0.00 B: 4.00 55 NOTOX Proiect 242933 CLINICAL SIGNS, DAILY (SUMMARY) FEMALES GROUP 4 SIGN (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. SKIN / FOR / PLOMAGE SCABS (3) (NECK) G ........................... . %: ......................... 3. RED STAINING (3) (EYE RIGHT) G: ...11...... I............... %: ...22...................... RED STAINING (3) (PERIORBITAL REGION RIGHT) G: .....11......... 1111....... %: .....22......... 3333....... SECRETION / EXCRETION . WATERY DISCHARGE FROM EYE (3) G: ........ .....11............ (EYE RIGHT) %: ............. 23........... BACK TO MAIN S Y M - S U M - 2,0 2 5 - M A R - 99 G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) 56 BACK TO MAIN NOTOX Proiect 242933 S Y M - S U M - 2.1 1 7 - M A R - 99 CLINICAL SIGNS (SUMMARY) FEMALES GROUP 5 (PFHS) ` SIGN (MAX.GRADE) treatment WEEKS: 1 ............ 2 ............ 3 ............ 4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1. TIME: ASABABABABABABABABABABABABABABABABABABABABABABABABABABABAB POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (SHOULDER RIGHT) ALOPECIA (3) (HINDLEG RIGHT) SCABS (3) (NECK) WOUND (3) (NECK) SECRETION / EXCRETION SALIVATION (3) G: %: G: ..................... 11111112111111. %: ................. 11111111111111. G: ..111111111222222111111111111111111111111112222222222222.. %.: ..111111111111111111113333333344333322222222211111111111.. G: .1111111111222222222222222222222222111111111111 %: .1111111111111111111111111111111111111111111111 G: %: G: %: G: 1111112222111111111111222222111111111111111111111111111. % : 1111111111111111111111111111111111111111111111111111111. G: ....... 11.. 111112222211. %: ..... 11..111111111111. G: %: G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) Time after treatment (H.Mi) A: 0.00 B: 4.00 57 NOTOX Prolect 242933 . CLINICAL SIGNS, DAILY (SUMMARY) FEMALES GROUP 5 (PFHS) SIGH (MAX.GRADE) LOCATION RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. * SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (HINDLEG RIGHT) G: ......................... 11 %: ......................... 33 G: 111..1111111U1111111111111 % : 122..22222333337777777777777 G: 111--- 111111111111111111111 %: 122--- .222222223333333333333 G: ..... 1111.................. %: ..... 2222.................. - BACK TO MAIN SYM-SUM - 22 25-MAR-99 : . G: Median value of the highest individual daily grades %: Percent of affected animals {0 = less than 5%, 1 = between 5% and 15%,.... 58 A = more than 95%) BACK TO MAIN NOTOX Project 242933 SYM-SUM - 13 17 -MA?.- 99 CLINICAL SIGNS (SUMMARY) FEMALES GROUP 6 (PFOS) . SIG N (MAX.GRADE) TREATMENT hjEreyq. 2............ 2 ..... ...... 3........... 4............ 5. days:* TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB POSTORE HUNCHED POSTURE ) SKIN / FOR / PLUMAGE ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (FORELEG LEFT) ALOPECIA (3) (FORELEG RIGHT) SCABS (3) (NECK) RED STAINING (3) (HEAD) RED STAINING (3) (EAR LEFT) RED STAINING (3) (EAR RIGHT) RED STAINING (3) (SNOOT) RED STAINING (3) (NECK) G: %: G: %: G: %: G: %: G: %: G: %: G: %: G: %: G: %: G: %: 11111111 11111111 1111111111111111111111111111...111111111111 1111111111111111111111111111...111111111111 ......................... 111111112. . 222222222222222.. ........... 111111111..111111111111111.. ........... 222222222..222222222222222.. ........... 111111111..111111111111111.. ................. 1111111........... ................. 1111111........... 1111.. . 1111.. . lili lili G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) Time after treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Proiect 242933 CLINICAL SIGNS, DAILY (SUMMARY) FEMALES GROUP 6 (PFOS) S IG N (MAX.GRADE) LOCATION RECOVERY WEEKS'. 1 ..... 2 ..... 3 ..... 4 ..... 5. SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) RED STAINING (3) (HEAD) RED STAINING (3) (EAR LEFT) RED STAINING (3) (EAR RIGHT) RED STAINING (3) (NECK) G: 11111........ %: 12222....... G: 11111111111111 %: 12255332222233 G: ...111111111 %: ...222222222 G: 111.. %: .222.. ........... 11111 %: 22222 BACK TO MAIN SYM-SUM - 14 2 5 - M A R - 99 } ! G: Median value of the highest individual daily grades %: Percent of affected animals {0 = less than 5%, 1 = between 5% and 1 5 % , A = more than 95%) 60 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS ' BACK TO MAIN BW-SUM - 1 09-DEC-98 BODY WEIGHTS (GRAM) SUMMARY MALES TREATMENT DAY 1 WEEK 1 MEAN ST.DEV. N DAY 5 WEEK 1 DAY 8 WEEK 2 DAY 12 WEEK 2 DAY 15 WEEK 3 DAY 19 WEEK 3 DAY 22 WEEK 4 DAY 25 WEEK 4 . DAY 28 WEEK 4 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 208 14.0 14 232 14.9 14 248 19.8 14 272 20.7 14 281 25.2 14 302 26.8 14 312 27.4 14 324 29.8 14 336 29.9 14 GROUP 2 TELOMER ALCOHOL 212 13.5 14 237 14.4 14 252 17.6 14 277 18.6 14 288 22.9 14 308 23.2 14 316 23.6 14 329 25.4 14 340 27.5 14 GROUP 3 TELOMER ACRYLATE GROUP 4 209 210 9.2 9.4 14 14 234 8.9 14 248 11.8 14 273 9.3 14 283 10.9 14 304 10.3 14 315 9.5 14 328 12.6 14 338 13.0 14 238 10.8 14 252 13.2 14 279 17.7 14 288 19.4 14 312 24.1 14 319 26.5 14 333 28.4 14 347 31.6 14 GROUP 5 PFHS 208 13.8 14 231 16.5 14 246 23.4 14 270 24.5 14 275 26.3 14 301 30.4 14 307 31.9 14 321 34.8 14 328 37.1 14 GROUP 6 PFOS 210 10.9 14 237 13.9 14 252 17.8 14 279 21.0 14 288 25.2 14 310 29.0 14 318 31.4 14 333 33.5 14 342 33.7 14 * / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level 61 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -_ PFHS/PFOS BACK TO MAIN BW-SUM - 2 . 09-DEC-98 BODY WEIGHTS (GRAM) SUMMARY MALES RECOVERY DAY 1 WEEK 1 DAY 5 WEEK 1 DAY 6 WEEK 2 DAY 12 WEEK 2 DAY 14 WEEK 2 DAY 19 WEEK 3 DAY 22 WEEK 4 DAY 25 WEEK 4 DAY 28 WEEK 4 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL 304 27.9 6 338 31.2 6 346 32.8 6 358 32.8 6 362 32.4 6 391 24.6 3 399 24.2 3 412 25.2 3 415 26.7 3 298 20.2 6 331 28.8 6 338 28.1 6 350 28.5 6 351 28.6 6 375 . 35.7 3 382 33.5 3 391 38.5 3 391 37.1 3 GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS 314 12.4 6 351 23.5 6 356 20.9 6 372 21.0 6 374 20.8 6 389 7.2 3 399 5.5 3 407 7.8 3 412 2.6 3 330 28.2 6 372 31.8 6 383 35.2 6 400 36.9 6 398 38.0 6 419 35.0 3 426 37.6 3 437 38.7 3 435 37.6 3 291 40.4 6 327 45.4 6 338 46.9 6 344 48.5 -6 345 47.6 6 347 19.1 3 351 19.1 3 368 20.8 3 362 20.8 3 GROUP 6 PFOS 313 40.4 6 352 46.8 6 360 46.0 6 374 46.8 6 376 47.6 6 351 39.7 3 359 38.6 3 371 34.8 3 370 39.3 3 * / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/. -PFHS/PFOS ' BACK TO MAIN BW-SUM - 3 09-DEC-98 BODY WEIGHTS (GRAM) SUMMARY FEMALES TREATMENT DAY 1 WEEK 1 DAY 5 WEEK 1 DAY 8 WEEK 2 DAY 12 WEEK 2 DAY 15 WEEK 3 DAY 19 WEEK 3 DAY 22 WEEK 4 DAY 25 WEEK 4 DAY 28 WEEK 4 MEAN ST.DEV. N MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST. DEV. N MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL 174 11.9 14 181 . 14.3 14 188 15.6 14 202 16.2 14 . 206 19.8 14 219 20.1 14 221 19.4 14 232 21.4 14 236 21.8 14 169 8.0 14 176 8.5 14 182 11.9 14 194 11.9 14 200 15.0 14 213 15.5 14 216 15.7 14 227 16.5 14 230 16.9 14 GROUP 3 TELOMER ACRYLATE GROUP 4 166 10.0 14 173 11.2 14 179 13.2 14 191 13.3 14 192 15.0 14 203 15.5 14 207 16.9 14 218 17.3 14 219 * 17.0 14 170 11.9 14 177 14.5 14 182 15.9 14 198 16.8 14 202 18.2 14 214 19.6 14 218 20.4 14 230 18.6 14 231 20.5 14 GROUP 5 PFHS 169 8.0 14 174 10.4 14 180 9.8 14 194 10.9 14 196 11.3 14 209 11.3 14 212 12.6 14 222 13.0 14 224 12.6 14 GROUP 6 PFOS 166 8.7 14 171 9.8 14 176 10.1 14 188 * 9.8 14 187 * 11.5 14 198 ** 12.7 14 200 ** 13.2 14 207 ** 14.3 14 207 * 13.3 14 * / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level 63 . NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ .,, PFHS/PFOS BACK TO MAIN BW-SUM - 4 09-DEC-98 BODY WEIGHTS (GRAM) SUMMARY FEMALES RECOVERY GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS DAY 1 WEEK 1 DAY 5 WEEK 1 DAY 8 WEEK 2 DAY 12 WEEK 2 DAY 14 WEEK 2 DAY 19 WEEK 3 DAY 22 WEEK 4 DAY 25 WEEK 4 DAY 28 WEEK 4 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 213 21.9 6 239 24.1 6 244 25.1 6 251 25.8 6 257 27.3 6 254 27.5 3 258 28.0 3 263 28.6 3 268 27.8 3 200 9.9 6 226 11.3 6 229 12.7 6 238 12.3 6 237 16.7 6 246 13.2 3 251 12.9 3 251 10.8 3 251 12.7 3 193 16.2 6 219 18.2 6 222 19.9 6 233 20.2 6 231 20.6 6 245 26.6 3 248 25.3 3 249 25.4 3 248 26.0 3 212 23.9 6 236 20.3 6 240 21.1 6 248 23.0 6 248 20.0 6 253 29.1 3 258 30.3 3 265 31.0 3 262 35.5 3 206 12.8 6 231 14.4 6 235 14.1 6 243 12.8 6 241 13.6 6 243 12.9 3 246 14.5 3 254 17.3 3 254 13.2 3 185 * 7.0 6 207 * 9.5 6 213 * ' 8.6 6 219 * ' 8.2 6 220 ** 5.5 6 226 11.3 3 224 9.8 3 226 10.7 3 229 13.0 3 * / ** : Dunnett-Test based on pooled variance significant at 5% (*) or lit (**) level 64 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS ' BACK TO MAIN BWG-SUM - 1 09-DEC-98 BODY WEIGHT GAIN (%) SUMMARY MALES TREATMENT DAY 1 WEEK 1 DAY 5 WEEK 1 DAY 8 WEEK 2 DAY 12 WEEK 2 DAY 15 WEEK 3 DAY 19 WEEK 3 DAY 22 WEEK 4 DAY 25 WEEK 4 DAY 28 WEEK 4 MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 0 0.0 14 12 6.3 14 19 7.4 14 31 7.9 14 35 9.5 14 . 45 10.1 14 50 10.4 14 56 11.2 14 61 11.4 14 GROUP 2 TELOMER ALCOHOL 0 0.0 14 12 1.9 14 19 3.3 14 30 4.8 14 36 6.5 14 45 7.6 14 49 7.9 14 55 8.8 14 60 9.1 14 GROUP 3 TELOMER ACRYLATE GROUP 4 00 0.0 0.0 14 14 12 13 1.5 2.3 14 14 19 20 3.7 3.5 14 14 31 33 4.7 5.5 14 14 35 37 5.0 6.5 14 14 45 48 5.8 9.0 14 14 51 52 5.7 9.9 14 14 57 58 6.5 11.4 14 14 62 65 7.1 12.9 14 14 GROUP 5 PFHS 0 0.0 14 11 2.2 14 18 4.6 14 30 4.5 14 32 7.2 14 45 6.7 14 48 7.3 14 54 8.5 14 58 9.6 14 GROUP 6 PFOS 0 0.0 14 13 1.5 14 20 4.2 14 33 4.8 14 37 7.4 14 47 8.9 14 51 10.3 14 58 10.8 14 62 11.1 14 * / * : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level 65 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS " BACK TO MAIN BWG-SUM - 2 09-DEC-98 BODY WEIGHT GAIN (%) SUMMARY MALES RECOVERY DAY 1 WEEK 1 DAY 5 WEEK 1 DAY 8 WEEK 2 DAY 12 WEEK 2 DAY 14 WEEK 2 DAY 19 WEEK 3 DAY 22 WEEK 4 DAY 25 WEEK 4 DAY 28 WEEK 4 MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 51 7.9 6 68 9.7 6 72 9.8 6 78 9.1 6 80 9.0 6 92 6.4 3 97 5.7 3 103 6.2 3 105 6.5 3 GROUP 2 TELOMER ALCOHOL 45 5.8 6 61 8.7 6 65 8.6 6 71 8.2 6 71 8.9 6 77 13.0 3 80 12.0 3 84 14.2 3 84 * 13.5 3 GROUP 3 TELOMER ACRYLATE GROUP 4 51 57 3.8 10.6 66 69 78 7.4 12.3 66 72 83 6.8 12.8 66 79 91 7.3 14.1 66 80 90 7.0 15.8 66 88 93 7.1 5.8 33 93 97 6.3 6.8 33 97 102 6.9 7.3 33 99 101 6.1 7.6 33 GROUP 5 PFHS 45 12.6 6 63 13.9 6 69 14.4 6 72 15.6 6 72 15.2 6 77 7.1 3 79 * 7.2 3 87 9.9 3 84 * 8.1 3 GROUP 6 PFOS 49 14.5 6 68 16.5 6 71 16.5 6 78 16.6 6 79 17.2 6 70 * 5.6 3 74 * 4.9 3 80 ' 2.7 3 80 * 4.5 3 / ** : Dunnett-Test based on pooled variance significant at 5H (*) or 1H (**) level 66 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS ' BACK TO MAIN BWG-SUM - 3 09-DEC-98 BODY WEIGHT GAIN (%) SUMMARY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS OAY 1 MEAN 0 0 00 0 0 WEEK 1 ST.DEV. 0.0 0.0 0.0 0.0 0.0 0.0 N 14 14 14 14 14 14 . DAY 5 MEAN 4 5 4 4 3 3 WEEK 1 ST.DEV. 2.7 2.5 2.3 2.3 3.2 3.4 N 14 14 14 14 14 14 DAY 8 MEAN 8 8 87 7 6 WEEK 2 ST.DEV. 3.2 ' 3.9 2.6 3.2 2.9 2.7 N 14 14 14 14 14 . 14 DAY 12 MEAN 16 15 15 17 14 13 WEEK 2 ST.DEV. 4.3 4.2 2.9 3.5 3.4 4.0 N . 14 14 14 14 14 14 DAY 15 MEAN 18 18 16 19 16 13 WEEK 3 ST.DEV. 5.5 5.8 4.3 4.2 3.7 4.6 N 14 14 14 14 14 14 DAY 19 MEAN 26 26 23 26 23 19 WEEK 3 ST. DEV. 5.6 5.8 5.3 4.2 3.9 6.4 N 14 14 14 14 14 14 DAY 22 MEAN 27 28 25 28 26 21 * WEEK 4 ST.DEV. 4.5 5.5 6.2 4.6 4.5 6.9 N 14 14 14 14 14 14 DAY 25 MEAN 33 34 31 35 31 24 ** WEEK 4 ST.DEV. 6.7 5.8 5.6 3.9 4.3 7.3 N 14 14 14 14 14 14 DAY 28 MEAN 36 36 32 36 33 25 ** WEEK 4 ST.DEV. 6.7 7.0 4.8 4.5 4.9 7.5 N 14 14 14 14 14 14 / * * . : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level 67 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ . -PFHS/PFOS ' BACK TO MAIN BWG-SUM - 4 09-DEC-98 BODY WEIGHT GAIN (%) SUMMARY FEMALES RECOVERY GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS DAY 1 MEAN 23 21 18 25 22 15 WEEK 1 ST.DEV. 5.7 3.7 7.7 4.4 5.0 6.1 N 6 6 .6 6 6 6 DAY 5 MEAN 37 37 35 39 37 29 WEEK 1 ST.DEV. 6.4 4.6 9.5 3.7 6.1 7.7 . N 6 6 666 6 DAY 8 MEAN 41 39 36 41 39 33 WEEK 2 ST.DEV. 7.0 5.4 9.9 '5.7 6.2 7.3 N 6 6 666 6 DAY 12 MEAN 44 44 43 46 44 37 WEEK 2 ST.DEV. 7.2 4.8 9.8 6.1 7.2 7.2 N 6 6 '6 6 6 6 DAY 14 MEAN 48 43 42 46 43 37 WEEK 2 ST.DEV. 8.8 7.8 9.8 4.6 8.5 5.9 N 6 6 666 6 DAY 19 MEAN 47 50 48 45 45 41 WEEK 3 ST.DEV. 12.5 7.0 4.8 5.1 8.6 10.3 N 3 3 333 3 DAY 22 MEAN 50 53 50 48 47 39 WEEK 4 ST.DEV. 12.1 6.7 6.2 3.7 9.5 9.5 N 3 3 333 3 DAY 25 MEAN 53 53 50 52 52 40 WEEK 4 ST.DEV. 12.7 5.4 3.8 2.8 7.6 10.0 N 3 3 333 3 DAY 28 MEAN 56 53 50 50 52 42 WEEK 4 ST.DEV. 13.8 6.2 3.0 5.6 8.8 11.5 N 3 3 333 3 / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1* (**) level 68 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -_ PFHS/PFOS BACK TO MAIN FC-SUM - 1 0 1 -MAR-99 FOOD CONSUMPTION (G/ANIMAL/DAY) SUMMARY MALES TREATMENT DAYS 1-8 WEEKS 1/2 DAYS 8-15 WEEKS 2/3 DAYS 15-22 WEEKS 3/4 DAYS 22-28 WEEK 4 MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) GROUP 1 VEHICLE CONTROL 26 2.1 4 28 2.7 4 30 ' 3.3 4 30 2.3 4 GROUP 2 TELOMER ALCOHOL ' GROUP 3 TELOMER ACRYLATE GROUP 4 26 27 26 1.9 1.0 1.0 4 44 28 28 29 2.4 0.9 0.6 4 44 29 30 30 1.3 1.5 2.3 444 30 31 . 31 1.5 1.1 2.4 4 44 GROUP 5 PFHS 26 2.4 4 27 2.1 4 29 1.2 4 30 1.6 4 GROUP 6 PFOS 27 0.9 4 29 1.6 4 30 2.7 4 31 3.0 4 MEAN OF MEANS OVER TREATMENT MEAN GROUP 1 VEHICLE CONTROL 28 GROUP 2 TELOMER ALCOHOL 28 GROUP 3 TELOMER ACRYLATE GROUP 4 29 29 GROUP 5 PFHS 28 GROUP 6 PFOS 29 / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS ' BACK TO MAIN FC-SUM - 2 01-MAR-99 FOOD CONSUMPTION (G/ANIMAL/DAY) SUMMARY MALES RECOVERY DAYS 1-8 WEEKS 1/2 DAYS 8-14 WEEK 2 DAYS 14-19 WEEKS 2/3 DAYS 19-22 WEEKS 3/4 DAYS 22-28 WEEK 4 MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) GROUP 1 VEHICLE CONTROL 30 2.5 2 30 1.8 2 30 --- 1 31 --- 1 31 -- 1 GROUP 2 TELOMER ALCOHOL 28 2.0 2 28 1.8 2 28 .. 1 29 --- 1 28 --- 1 GROUP 3 TELOMER ACRYLATE GROUP 4 30 32 1.8 1.4 22 31 31 1.4 2.0 22 30 30 --- - .- 11 32 31 ... 11 30 - - 29 --- - 11 GROUP 5 PFHS 30 0.8 2 29 1.2 2 26 ... 1 27 ... 1 27 ... 1 GROUP 6 pros 30 3.7 2 30 4.0 2 27 ... 1 28 ... 1 27 --- . 1 MEAN OF MEANS OVER RECOVERY MEAN GROUP 1 VEHICLE CONTROL 30 GROUP 2 TELOMER ALCOHOL 28 GROUP 3 TELOMER ACRYLATE GROUP 4 30 30 GROUP 5 PFHS 28 GROUP 6 PFOS 28 * / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (*') level 70 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS . BACK TO MAIN FC-SUM - 3 01-MAR-99 FOOD CONSUMPTION (G/ANIMAL/DAY) SUMMARY FEMALES TREATMENT DAYS 1-8 WEEKS 1/2 DAYS 8-15 WEEKS 2/3 DAYS 15-22 WEEKS 3/4 DAYS 22-28 WEEK 4 MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) GROUP 1 VEHICLE CONTROL 19 0.7 4 21 0.5 4 22 1.3 4 24 1.2 4 GROUP 2 TELOMER ALCOHOL 18 1.2 4 20 0.5 4 22 0.8 4 23 0.7 4 GROUP 3 TELOMER ACRYLATE GROUP 4 19 19 1.2 0.6 44 20 21 1.1 0.8 44 22 22 1.2 1.1 44 23 23 1.3 0.5 44 GROUP 5 PFHS 20 1.6 4 21 1.4 4 22 1.3 4 23 0.7 4 GROUP 6 PFOS 19 1.9 4 19 0.8 4 20 * 1.0 4 20 ** 0.5 4 MEAN OF MEANS OVER TREATMENT MEAN GROUP 1 VEHICLE CONTROL 22 GROUP 2 TELOMER ALCOHOL 21 GROUP 3 TELOMER ACRYLATE GROUP 4 21 21 GROUP 5 PFHS 22 GROUP 6 PFOS 19 * / ** : Dunnett-Test based on pooled variance significant at 5% () or 1% (**) level 71 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -_ PFHS/PFOS BACK TO MAIN FC-SUM - 4 0 1 -MAR-99 F O O D C O N S U M P T I O N (G/ANIMAL/DAY) S U M M A R Y FEMALES RECOVERY DAYS 1-8 WEEKS 1/2 DAYS 8-14 WEEK 2 DAYS 14-19 WEEKS 2/3 DAYS 19-22 WEEKS 3/4 DAYS 22-28 WEEK 4 MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) GROUP 1 VEHICLE CONTROL 25 0.4 2 24 0.2 2 22 ..1 24 ... 1 24 ... 1 GROUP 2 TELOMER ALCOHOL 24 1.0 2 23 1.1 2 22 ... 1 23 ..1 22 -- 1 GROUP 3 TELOMER ACRYLATE GROUP 4 24 24 1.2 1.1 22 23 23 0.8 1.2 22 22 20 ... ... 11 22 21 ... ... 11 22 22 ... ... 11 GROUP 5 PFHS 24 0.6 2 24 0.6 2 20 1 22 1 21 1 GROUP 6 PFOS 21 ** 0.2 2 20 * 0.3 2 19 1 19 1 19 1 MEAN OF MEANS OVER RECOVERY MEAN GROUP 1 VEHICLE CONTROL 24 GROUP 2 TELOMER ALCOHOL 23 GROUP 3 TELOMER ACRYLATE GROUP 4 22 22 GROUP 5 PFHS 22 GROUP 6 PFOS 20 / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS' BACK TO MAIN Kl-U-UM - 1 0 1 -MAR-99 RELATIVE FOOD CONSUMPTION SUMMARY (G/KG BODY WEIGHT/DAY) MALES TREATMENT DAYS 1-8 WEEKS 1/2 DAYS 8-15 WEEKS 2/3 DAYS 15-22 WEEKS 3/4 DAYS 22-28 WEEK 4 MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) GROUP 1 VEHICLE CONTROL 114 8.7 4 103 9.3 4 99 11.1 4 93 6.8 4 GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 112 3.6 4. 102 3.3 4 96 4.5 4 91 1.1 4 115 3.8 4 103 2.0 4 99 5.2 4 94 3.4 4 111 2.9 4 102 2.3 4 96 5.9 4 92 5.3 4 GROUP 5 PFHS 112 7.1 4 101 2.6 4 98 5.5 4 93 3.6 4 GROUP 6 PFOS 115 3.7 4 104 1.9 4 98 6.8 4 94 6.1 4 MEAN OF MEANS OVER TREATMENT MEAN GROUP 1 VEHICLE CONTROL 102 GROUP 2 TELOMER ALCOHOL 100 GROUP 3 TELOMER ACRYLATE GROUP 4 103 100 GROUP 5 GROUP 6 PFHS ' PFOS 101 103 / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -. PFHS/PFOS BACK TO MAIN RFC-SUM - 2 01-MAR-99 RELATIVE FOOD CONSUMPTION SUMMARY (G/KG BODY WEIGHT/DAY) MALES RECOVERY DAYS 1-8 WEEKS 1/2 DAYS 8-14 WEEK 2 DAYS 14-19 WEEKS 2/3 DAYS 19-22 WEEKS 3/4 DAYS 22-28 WEEK 4 MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N .(CAGE) MEAN ST.DEV. N (CAGE) GROUP 1 VEHICLE CONTROL 90 1.5 2 84 0.1 2 77 --1 78 ... 1 75 -- 1 GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 85 87 0.9 4.9 2 2 80 82 0.7 2.6 22 74 76 . . . 11 76 80 -- . 11 72 74 -- ... 11 85 4.1 2 78 5.8 2 71 ... 1 72 ... 1 67 ... 1 GROUP 5 PFHS 91 0.3 2 83 0.1 2 76 ... 1 77 ... 1 73 ... 1 GROUP 6 pros 86 2.1 2 81 0.2 2 76 ... 1 78 ... 1 72 ... MEAN OF MEANS OVER RECOVERY ' MEAN GROUP 1 VEHICLE CONTROL 81 GROUP 2 TELOMER ALCOHOL 77 GROUP 3 TELOMER ACRYLATE GROUP 4 80 74 GROUP 5 PFHS 80 GROUP 6 PFOS 79 * / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level 74 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS '' BACK TO MAIN RFC-SUM 3 01-MAR-99 RELATIVE FOOD CONSUMPTION SUMMARY (G/KG BODY WEIGHT/DAY) FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 DAYS 1-8 WEEKS 1/2 DAYS 8-15 WEEKS 2/3 DAYS 15-22 WEEKS 3/4 DAYS 22-28 WEEK 4 MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) 106 1.2 4 104 2.4 4 103 6.8 4 102 6.1 4 104 6.1 4 105 2.8 4 105 6.7 4 102 4.6 4 110 5.0 4 107 4.7 4 108 5.7 4 105 2.8 4 108 5.4 4 107 4.7 4 105 6.2 4 101 3.4 4 GROUP 5 PFHS 113 11.8 4 109 8.0 4 107 4.9 4 104 1.9 4 GROUP 6 PFOS 111 11.4 4 103 6.4 4 100 7.9 4 95 4.6 4 MEAN OF MEANS OVER TREATMENT MEAN GROUP 1 VEHICLE CONTROL 104 GROUP 2 TELOMER ALCOHOL 104 GROUP 3 TELOMER ACRYLATE GROUP 4 107 105 GROUP 5 PFHS 108 GROUP 6 PFOS 102 / ** : Dunnett-Test based on pooled variance significant at 5% {*) or 1% (**) level NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS " BACK TO MAIN RFC-SUM - 4 01-MAR-99 RELATIVE FOOD CONSUMPTION SUMMARY (G/KG BODY WEIGHT/DAY) FEMALES RECOVERY DAYS 1-8 WEEKS 1/2 DAYS 8-14 WEEK 2 DAYS 14-19 WEEKS 2/3 DAYS 19-22 WEEKS 3/4 DAYS 22-28 WEEK 4 MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) MEAN ST.DEV. N (CAGE) GROUP 1 VEHICLE CONTROL 105 3.5 2 95 0.2 2 86 -- 1 92 1 91 1 GROUP 2 TELOMER ALCOHOL 104 2.7 2 95 3.7 2 88 -- 1 92 -- 1 86 --1 GROUP 3 TELOMER ACRYLATE GROUP 4 107 102 2.4 5.3 22 97 94 1.0 4.0 22 88 81 ---- 11 87 83 ---- 11 90 81 ---- 11 GROUP 5 PFHS 106 1.2 2 97 0.2 2 84 -- 1 88 -- 1 84 -- 1 GROUP 6 PFOS 100 4.0 2 93 3.9 2 85 --1 86 -- 1 85 .-1 MEAN OF MEANS OVER RECOVERY MEAN GROUP 1 VEHICLE CONTROL 94 GROUP 2 TELOMER ALCOHOL 93 GROUP 3 TELOMER ACRYLATE GROUP 4 94 88 GROUP 5 PFHS 92 GROUP 6 PFOS 90 * / * : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level ----------- - NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ . -PFHS/PFOS " BACK TO MAIN HEM-SUM - 1 0 9 -NOV-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS MALES TREATMENT HAEMATOLOGY PARAMETERS RBC T/1 HB mmol/I HCT 1/1 MCV fl MCH fmol MCHC mmol/I ROW % PLATELETS G/l WBC G/l SEG. 1 EO. 1 BASO. 1 MONO. 1 LYMPH. 1 MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN S T '.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS 7.05 0.24 10 9.1 0.4 10 0.407 0.011 10 57.8 1.1 10 1.294 0.036 10 22.4 0.4 10 12.4 0.4 10 1182 110 10 11.3 3.5 10 0.124 0.033 10 0.007 0.005 10 0.000 0.000 10 0.026 0.006 10 0.844 0.030 10 6.72 0.32 14 8.8 0.4 14 0.395 0.016 14 58.8 1.4 14 1.313 0.030 14 22.3 0.4 14 12.0 0.7 14 1025 116 14 10.0 2.0 14 0.125 0.041 14 0.011 0.011 14 0.000 0.000 14 0.022 0.012 14 0.841 0.048 14 6.61 * 0.27 14 6.79 0.32 13 8.7 8.8 0.4 0.5 14 13 0.388 0.014 14 0.397 0.021 13 58.7 1.0 14 58.5 1.2 13 1.317 0.027 14 1.297 0.045 13 22.4 0.5 14 22.2 0.4 13 12.2 0.6 14 12.0 0.3 13 1115 158 14 1050 158 13 10.2 2.1 14 11.7 2.4 13 0.117 0.035 14 0.087 + 0.025 13 0.007 0.005 14 0.007 0.004 - 13 0.000 0.000 14 0.000 0.000 13 0.020 0.010 14 0.023 0.009 13 0.857 0.040 14 0.883 + 0.031 13 6.57 '* 0.52 14 8.5 ** 0.6 14 0.383 * 0.026 14 58.4 1.8 14 1.297 0.035 14 22.2 0.4 14 12.0 0.7 14 870 ** 306 14 9.7 3.0 14 0.148 0.049 14 0.008 0.007 14 0.000 0.000 14 0.026 0.013 14 0.818 0.053 14 6.66 0.39 12 8.9 0.4 12 0.392 0.019 12 59.0 1.9 12 1.338 0.047 12 22.7 0.4 12 12.0 0.8 12 1092 75 12 10.7 2.2 12 0.114 0.039 12 0.005 0.005 12 0.000 0.000 12 0.023 0.009 12 0.858 0.041 12 */**: Dunnett -test based on pooled variance s1g. at 5% or 1% level . + : Steel test slg. at 5% level. 77 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/' -PFHS/PFOS " ^ ' BACK TO MAIN HEM-SUM 2 0 9 -NOV-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS MALES TREATMENT GROUP 1 VEHICLE CONTROL HAEMATOLOGY PARAMETERS PT MEAN SEC ST.DEV. N PTT MEAN SEC ST.DEV. N 14.9 0.6 13 16.1 0.8 13 GROUP 2 TELOMER ALCOHOL 15.0 0.6 14 16.0 0.7 14 GROUP 3 TELOMER ACRYLATE GROUP 4 14.9 0.6 13 17.1 2.2 13 14.4 0.6 12 18.2 3.2 11 GROUP 5 PFHS 14.1 ** 0.4 9 16.8 0.8 9 GROUP 6 PFOS 14.7 0.6 13 17.2 1.8 13 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level. 78 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ 'PFHS/PFOS BACK TO MAIN HEM-SUM - 3 09-N0V-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS HAEMATOLOGY PARAMETERS RBC MEAN 6.72 6.27 '* 6.61 6.62 6.35 * 6.41 T/l ST.OEV. 0.14 0.39 0.34 0.36 0.28 0.37 N 11 13 13 13 13 11 HB MEAN 9.1 8.5 * 8.8 9.0 8.7 8.6 m m o 1/1 ST.OEV. 0.3 0.5 0.5 0.4 0.4 0.5 N 11 13 13 13 13 11 HCT MEAN 0.378 0.355 ** 0.370 0.377 0.358 * 0.359* 1/1 ST.OEV. 0.012 0.018 0.019 0.014 0.017 0.019 N 11 13 13 13 13 11 MCV n MCH fmol MEAN ST.OEV. N MEAN ST.OEV. N 56.2 1.9 11 1.350 0.034 11 56.6 1.4 13 1.356 0.035 13 56.0 1.7 13 1.329 0.049 13 57.1 1.9 13 1.363 0.053 13 56.3 2.6 13 1.356 0.050 13 56.0 1.9 11 1.348 0.041 11 MCHC mmol/I MEAN ST.OEV. N 24.0 0.5 11 24.0 0.6 13 23.7 0.6 13 23.9 0.5 13 24.3 0.5 13 24.1 0.2 11 ROW MEAN 11.3 11.2 11.4 11.3 11.3 11.2 % ST.DEV. 0.4 0.5 0.6 0.3 0.6 0.5 N 11 13 13 13 13 11 PLATELETS G/l MEAN ST.DEV. N 1166 127 11 1141 149 13 1149 125 13 1169 74 13 1035 236 13 1134 131 11 WBC ` MEAN 7.4 7.5 6.8 7.6 6.3 8.4 G/l ST.DEV. 2.1 1.6 2.3 1.8 1.9 2.4 N 11 13 13 13 13 11 SEG. 1 EO. 1 MEAN ST.DEV. N MEAN ST.DEV. N 0.082 0.027 11 0.010 0.007 11 0.103 0.041 13 0.004 0.005 13 0.109 0.039 13 0.009 0.009 13 0.087 0.024 13 0.011 0.006 13 0.080 ' 0.035 13 0.009 0.006 13 0.077 0.020 11 0.005 0.004 11 BASO. 1 MEAN ST.DEV. N 0.000 0.000 11 0.000 0.000 13 0.000 0.000 13 0.000 0.000 13 0.000 0.000 13 0.000 0.000 11 MONO. 1 MEAN ST.DEV. N 0.025 0.011 11 0.020 0.011 13 0.017 0.009 13 0.024 0.008 13 0.024 0.012 13 0.020 0.007 11 LYMPH. MEAN 0.883 0.873 0.865 0.877 0.887 0.898 1 ST.DEV. 0.034 0.044 0.040 0.030 0.039 0.026 N 11 13 13 13 13 11 */*': Dunnett test based on pooled variance s1g. at 5% or 1% level . +: Steel test s1g. at 5% level. 79 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ '" PFHS/PFOS BACK TO MAIN HEM-SUM - 4 09-NOV-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS FEMALES TREATMENT HAEMATOLOGY PARAMETERS PT MEAN SEC ST.DEV. N PTT MEAN SEC ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS 15.4 1.0 14 15.9 1.4 14 15.0 0.6 14 15.0 0.9 14 15.0 0.8 13 15.7 0.8 12 14.5 % 0.5 13 17.0 0.9 13 14.5 0.7 12 16.8 2.0 11 GROUP 6 PFOS 14.4 0.4 14 16.5 2.2 14 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level. 80 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS ' BACK TO MAIN HEM-SUM - 5 0 9 -D E C -98 HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT HAEMATOLOGY PARAMETERS GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS RBC MEAN 7.23 7.04 6.97 7.00 6.97 7.01 T/l ST.DEV. 0.18 0.25 0.27 0.27 0.21 0.22 N 3 3 333 3 HB mmol/1 HCT 1/1 MEAN ST.DEV. N MEAN ST.DEV. N 9.1 0.4 3 0.417 0.011 3 8.9 0.1 3 0.404 0.008 3 8.7 0.5 3 0.401 0.018 3 8.8 0.1 3 0.402 0.014 3 8.6 0.1 3 0.392 0.004 3 8.9 0.0 3 0.408 0.006 3 MCV MEAN 57.7 57.3 57.5 57.4 56.3 58.2 fl ST.DEV. 0.1 1.0 0.6 0.3 1.6 1.0 N 33 333 3 MCH fmol MEAN ST.DEV. N 1.259 0.036 3 1.260 0.037 3 1.253 0.028 3 1.259 0.034 3 1.230 0.020 3 1.270 0.041 3 MCHC mmol/1 MEAN ST.DEV. N 21.8 0.6 3 22.0 0.3 3 21.8 0.3 3 21.9 0.5 3 21.8 0.3 3 21.8 0.3 3 RDW MEAN 14.1 13.7 13.4 13.3 14.4 13.1 % ST.DEV. 0.5 0.6 0.8 1.3 0.5 0.8 N 33 333 3 PLATELETS G/l MEAN ST.DEV. N 1177 106 3 1081 102 3 1186 170 3 1080 49 3 1053 22 3 1078 58 3 WBC MEAN 8.5 12.0 12.1 12.2 8.5 11.6 G/l ST.DEV. 1.8 3.2 1.9 1.8 2.4 1.9 N 3 3 333 3 SEG. 1 MEAN ST.DEV. N 0.112 0.043 3 0.127 0.073 3 0.088 0.048 3 0.058 0.019 3 0,133 0.063 3 0.137 0.016 3. EO. MEAN 0.013 0.005 0.005 0.003 0.012 0.002 1 ST.DEV. 0.003 0.005 0.005 0.003 0.003 0.003 N 33 333 3 BASO. 1 MEAN ST.DEV. N 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 MONO. 1 MEAN ST.DEV. N 0.027 0.003 3 0.022 0.008 3 0.025 0.005 3 0.023 0.006 3 0.018 0.008 3 0.018 0.003 3 LYMPH. MEAN 0.848 0.847 0.882 0.915 0.837 0.843 1 ST.DEV. 0.043 0.077 0.055 0.022 0.073 0.020 N 33 333 3 /**: Dunnett -test based on pooled variance s1g. at 5% or 1% level!. +: Steel -test sig. at 5% level 81 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ *PFHS/PFOS ' BACK TO MAIN HEM-SUM - 6 0 9 -DEC-98 HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT HAEMATOLOGY PARAMETERS PT MEAN SEC ST.DEV. N PTT MEAN SEC ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL 14.6 0.4 3 16.2 1.1 3 15.2 0.5 3 15.3 0.7 3 GROUP 3 TELOMER ACRYLATE GROUP' 4 GROUP 5 PFHS 14.9 0.7 3 16.0 1.0 3 14.6 0.6 3 15.0 1.3 3 15.2 , 0.4 2 15.2 0.2 2 GROUP 6 PFOS 15.5 0.5 3 14.7 1.6 3 /**: Dunnett-test based on pooled variance $1g. at 5% or 1% level. +: Steel-test s1g. at 5% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ 'PFHS/PFOS BACK TO MAIN HEM-SUM - 7 0 9 -DEC-98 HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP PFOS HAEMATOLOGY PARAMETERS RBC MEAN 7.02 6.47 6.62 6.71 6.61 ' 6.53 T/l ST.DEV. 0.09 0.19 0.23 0.51 0.35 0.33 N 3 3 3. 3 3 3 HB MEAN 9.0 8.4 8.7 9.0 8.6 8.5 mmol/I ST.DEV. 0.2 0.2 0.3 0.4 0.5 0.4 N 333333 HCT MEAN 0.404 0.378 0.389 0.396 0.383 0.387 1/1 ST.DEV. 0.009 0.005 0.012 0.012 0.030 0.016 N 333333 MCV MEAN 57.5 58.4 58.9 59.2 57.9 59.3 fl ST.DEV. 0.7 1.7 2.8 2.7 2.1 0.6 N 333333 MCH fmol MEAN ST. DEV. N 1.277 0.021 3 1.305 0.060 3 1.310 0.055 3 1.339 0.048 3 1.301 0.041 3 1.307 0.012 3 MCHC mmol/I MEAN ST.DEV. N 22.2 0.2 3 22.4 0.4 3 22.3 0.2 3 22.6 0.4 3 22.5 0.5 3 22.1 0.2 3 ROW MEAN 14.5 15.2 15.4 15.3 14.6 15.7 % ST.DEV. 1.5 1.1 1.3 1.7 1.1 0.1 N 33333 3 PLATELETS G/l MEAN ST.DEV. N 1232 165 3 1117 144 3 1066 133 3 1111 56 3 854 * 145 3 1034 233 3 WBC MEAN 7.3 8.7 7.3 6.7 6.6 6.8 G/l ST.DEV. 1.8 2.8 3.3 1.3 1.0 2 .5 N 33 3333 SEG. 1 MEAN ST.DEV. N 0.075 0.035 3 0.077 0.020 3 0.065 0.018 3 0.080 0.033 3 0.050 0.030 3 0.100 0.015 3 EO. 'MEAN 0.005 0.007 0.003 0.010 0.008 0.002 1 ST.DEV. 0.005 0.008 0.003 0.005 0.010 0.003 N 333333 BASO. 1 MEAN ST.DEV. N 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 0.000 0.000. 3 0.000 0.000 3 0.000 0.000 3 MONO. 1 MEAN ST.DEV. N 0.017 0.008 3 0.025 0.010 3 0.017 0.013 3 0.033 0.013 3 0.010 0.009 3 0.027 0.008 3 LYMPH. MEAN 0.903 0.892 0.915 0.877 0.932 0.872 1 ST.DEV. 0.038 0.023 0.022 0.023 0.032 0.010 N 33 3333 */*: Dunnett -test based on pooled variance s1g. at 5% or 1% level . +: Steel -test s1g: at 5% levi 83 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ *PFHS/PFOS ' BACK TO MAIN HEM-SUM - 8 09-DFr.cjfl HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT HAEMATOLOGY PARAMETERS GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 . GROUP 5 PFHS GROUP 6 PFOS PT SEC MEAN ST.DEV. N 16.0 0.4 3 14.9 0.4 3 15.6 0.6 3 14.8 * 0.3 3 14.8 * 0.1 2 15.5 0.1 2 PTT SEC MEAN ST.DEV. N ' 15.5 2.0 3 14.4 0.3 3 15.1 1.1 3 14.8 0.9 3 15.7 1.3 2 13.7 0.0 2 */*: Dunnett-tast based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level. 84 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ 'PFHS/PFOS " BACK TO MAIN HEM-SUM - 9 09-DEC-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS HAEMATOLOGY PARAMETERS RBC MEAN 7.35 7.01 6.85 7.01 6.89 6.99 T/1 ST.DEV. 0.50 0.14 0.16 0.17 0.08 0.31 N 3 3 333 3 HB MEAN 9.1 9.1 9.1 9.2 9.0 9.0 mmol/1 ST.DEV. 0.5 0.2 0.3 0.3 0.4 0.2 N 33 333 3 HOT MEAN 0.396 0.391 0.384 0.392 0.390 0.389 1/1 ST.DEV. 0.026 0.005 . 0.012 0.015 0.014 0.015 N 3 3 333 3 MCV MEAN 53.9 55.8 56.1 55.8 56.7 55.7 fl ST.DEV. 1.6 0.8 1.6 0.8 1.4 1.8 N 33 333 3 MCH fmol MEAN ST.DEV. N 1.243 0.052 3 1.294 0.037 3 1.323 0.020 3 1.317 0.031 3 1.307 0.042 3 1.284 0.041 3 MCHC mmol/1 MEAN ST.DEV. N 23.1 0.3 3 23.2 0.3 3 23.6 0.4 3 23.6 0.6 3 23.1 0.2 3 23.1 0.3 3 ROW MEAN 14.5 12.9 * 13.7 12^9 * 13.6 13.7 % ST.DEV. 0.4 0.5 0.3 0.4 0.3 0.9 N 33 333 3 PLATELETS G/l MEAN ST.DEV. N 1082 113 3. 1025 95 3 1076 149 3 1179 70 3 966 71 3 1077 27 3 WBC MEAN 12.2 12.3 10.8 9.4 7.7 11.2 G/1 ST.DEV. 0.9 3.0 4.2 1.3 1.6 3.3 N 33 333 3 SEG. 1 MEAN ST.DEV. N 0.103 0.053 3 0.095 0.028 3 0.090 0.033 3 0.060 0.009 3 0.063 0.003 3 0.078 0.046 3 EO. MEAN 0.007 0.003 0.010 0.005 0.007 0.007 1 ST.DEV. 0.006 0.006 0.005 0.005 0.003 0.003 N 3 3 333 3 BASO. 1 MEAN ST.DEV. N 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 0.000 0.000 3 MONO. 1 MEAN ST.DEV. N 0.052 0.025 3 0.055 0.018 3 0.047 0.033 3 0.020 0.009 3 0.025 0.009 3 0.027 0.020 3 LYMPH. MEAN 0.838 0.847 0.853 0.915 0.905 0.888 1 ST.DEV. 0.056 0.050 0.068 0.005 0.013 0.059 N 33 333 3 */**: Dunnett -test based on pooled variance s1g. at 5% or 1% level . +: Steel test s1g. at 5% level 85 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ '/PFHS/PFOS " BACK TO MAIN HEM-SUM -10 09-DEC-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT HAEMATOLOGY PARAMETERS PT MEAN SEC ST.DEV. N PTT MEAN SEC ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS 13.1 0.6 2 16.0 0.1 2 13.0 0.4 2 15.2 0.8 2 13.4 0.2 3 16.8 0.2 3 13.8 1.1 2 16.5 0.8 2 14.2 0.5 2 15.2 0.4 2 14.0 0.2 3 14.6 2.0 3 */**: Dunnett-test based on pooled variance slg. at 5% or 1% level. +: Steel-test sig. at 5% level. 86 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ ' -' /PFHS/PFOS " BACK TO MAIN HEM-SUM -11 '09-DEC-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 GROUP 3 TELOMER . TELOMER ALCOHOL ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS HAEMATOLOGY PARAMETERS RBC MEAN 6.87 6.86 6.79 6.99 6.80 6.56 T/l ST.DEV. 0.19 0.09 0.27 0.22 0.25 0.15 N 333333 HB MEAN 9.3 9.4 9.0 9.1 8.9 8.7 mmol/1 ST.DEV. 0.1 0.2 ' 0.2 0.2 0.3 0.6 N 3 3 3 .3 3 3 HOT MEAN 0.397 0.402 0.389 0.393 0.389 0.379 1/1 ST.DEV. 0.023 0.002 0.009 0.008 0.022 0.030 N 3 33333 MCV MEAN 57.8 58.7 57.4 56.2 57.2 57.8 fl ST.DEV. 1.8 1.1 2.3 0.9 3.4 3.3 N 333333 MCH fmol MEAN ST.DEV. N 1.349 0.020 3 1.366 0.018 3 1.322 0.033 3 1.297 0.030 3 1.314 0.049 3 1.321 0.058 3 MCHC mmol/I MEAN ST.DEV. N 23.4 1.0 3 23.3 0.4 3 23.1 0.4 3 23.1 0.2 3 23.0 0.5 3 22.9 0.6 3 RDW MEAN 12.8 13.5 13.3 12.6 13.3 12.9 % ST.DEV. 0.8 0.5 0.2 0.3 1.1 0.9 N 333333 PLATELETS MEAN 972 980 965 1014 978 994 G/l ST.DEV. 54 103 126 40 127 80 N 333333 WBC MEAN 6.0 8.8 7.7 6.2 6.8 8.7 G/l ST.DEV. 0.6 1.1 3.7 0.4 2.3 2.3 N 333333 SEG. 1 MEAN ST.DEV. N 0.053 0.031 3 0.070 0.013 3 0.038 0.015 3 0.077 0.045 3 0.075 0.017 3 0.062 0.025 3 EO. 1 BASO. 1 MEAN ST.DEV. N MEAN ST.DEV. N 0.008 0.010 3 0.000 0.000 3 0.003 0.003 3 0.000 0.000 3 0.010 0.000 3 0.000 0.000 3 0.020 0.000 3 0.000 0.000 3 0.007 0.003 3 0.000 0.000 3 0.005 0.009 3 0.000 0.000 3 MONO. 1 MEAN ST.DEV. N 0.015 0.005 3 0.027 0.008 3 0.018 0.014 3 0.030 0.000 3 0.035 0.018 3 0.017 0.008 3 LYMPH. 1. MEAN ST.DEV. N 0.923 0.032 3 0.900 0.005 3 0.933 0.010 3 0.873 0.045 3 0.883 0.030 3 0.917 0.033 3 */**: Dunnett -test based on pooled variance s1g. at 5% or 1% level . +: Steel -test s1g. at 5% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ '/PFHS/PFOS BACK TO MAIN HEM-SUM -12 09-DEC-98 HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 GROUP 6 PFHS . PFOS HAEMATOLOGY PARAMETERS PT MEAN SEC ST.DEV. N PTT MEAN SEC ST.DEV. N 12.3 0.6 3 15.7 1.2 3 12.2 0.3 3 14.6 0.5 3 11.9 0.4 3 15.7 1.1 3 12.2 0.6 3 15.0 0.6 3 11.7 0.3 3 15.3 0.2 3 12.2 0.4 3 14.9 1.1 3 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level. 88 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ . -PFHS/PFOS ' "" BACK TO MAIN CHE-SUM - 1 09-N0V-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS MALES TREATMENT ALAT(GPT) ukat/1 ASAT(GOT) ukat/1 Bill T. umol/1 CHOLEST.T. mraol/1 TRIGL. mmol/1 CREATININE umol/1 GLUCOSE mmol/1 UREA mmol/1 PROTEIN T. o/i ALBUMIN g/i GLOBULIN 0/1 A/G RATIO ALP ukat/1 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 0.64 0.12 14 2.80 0.38 14 2 1 14 2.00 0.39 14 0.80 0.25 14 33. 4 14 6.32 0.75 14 6.9 1.1 14 56.8 2.9 14 . 29.3 0.9 14 27.5 2.5 14 1.1 0.1 14 7.76 1.75 14 GROUP 2 TELOMER ALCOHOL 0.61 0.10 14 2.62 0.41 14 2 0 14 1.28 ** 0.44 14 0.63 0.29 14 30 3 14 6.48 0.95 14 7.0 1.0 14 53.2 ** 2.1 14 28.7 1.3 14 24.4 + 1.8 14 1.2 0.1 14 8.08 1.94 14 GROUP 3 TELOMER ACRYLATE GROUP 4 0.61 0.14 13 2.58 0.46 14 2 1 14 1.43 ** 0.47 14 0.73 0.39 14 31 2 14 6.24 0.73 14 6.7 1.1 14 53.9 * 2.9 14 29.3 1.7 14 24.6 + 2.0 14 1.2 + 0.1 14 7.70 1.14 14 0.57 0.11 13 2.31 * 0.30 14 2 0 14 1.76 0.40 14 0.65 0.21 14 34 4 14 6.59 1.07 14 6.9 1.0 14 53.8 * 2.2 14 29.8 1.3 14 24.0 + 1.5 14 1.2 + 0.1 14 8.00 1.16 14 GROUP 5 PFHS 0.67 0.25 14 2.39 ' 0.26 14 2 0 14 0.97 ** 0.20 14 0.43 ** 0.15 14 35 5 14 6.72 1.47 14 7.7 1.5 14 53.7 ** 2.8 14 30.4 1.4 14 23.3 + 2.4 14 1.3 + 0.1 14 9.33 * 1.50 14 GROUP 6 PFOS 0.59 0.06 14 2.37 * 0.45 14 2 1 14 1.13 * 0.40 14 0.48 ** 0.17 14 32 3 14 6.38 1.24 14 7.6 1.2 14 54.1 * 2.0 14 29.3 1.2 14 24.8 + 2.1 14 1.2 + 0.1 14 7.26 1.48 14 */**: Dunnett -test based on pooled variance s1g. at 5% or 1% level. +: Steel-test slg. at 5% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ 'PFHS/PFOS " BACK TO MAIN CHE-SUM - 2 O9-N0V-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS MALES TREATMENT GROUP 1 VEHICLE CONTROL . GROUP 2 TELOMER ALCOHOL INORG PHOSPH MEAN mmol/1 ST.DEV. N SODIUM mmol/1 MEAN ST.DEV. N POTASSIUM mmol/1 MEAN ST.DEV. N CALCIUM mmol/1 MEAN ST.DEV. N CHLORIDE mmol/1 MEAN ST.DEV. N 2.83 0.16 14 142.6 1.3 14 4.85 0.31 14 2.53 0.08 14 100 2 14 2.75 0.13 14 ' 142.9 0.9 14 4.60 0.27 14 2.47 0.06 14 100 2 14 GROUP 3 TELOMER ACRYLATE GROUP 4 2.88 0.19 14 143.2 0.7 14 4.72 0.39 14 2.50 0.06 14 102 1 14 2.71 0.23 14 143.0 0.7 14 4.50 * 0.22 14 2.47 0.07 14 101 2 14 GROUP 5 PFHS 3.02 0.30 14 143.3 0.8 14 4.55 0.46 14 2.51 0.08 14 103 ** 2 14 GROUP PFOS 2.96 0.20 14 142.9 0.9 14 4.66 0.35 14 2.52 0.08 14 103 *' 3 14 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level. 90 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ 'PFHS/PFOS " BACK TO MAIN CHE-SUM 3 09-N0V-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS FEMALES TREATMENT ALAT(GPT) ukat/1 A S A T (G O T ) ukat/1 BILI T. umol/1 CHOLEST.T. mmol/1 TRIGL. mmol/1 CREATININE umol/1 GLUCOSE mmol/1 UREA mmol/1 PROTEIN T. gn ALBUMIN g/1 GLOBULIN g/1 A/G RATIO ALP ukat/1 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N. MEAN ST.DEV. N MEAN ST.OEV. N MEAN ST.DEV. N MEAN ST.OEV. N MEAN ST.DEV. N MEAN ST.OEV. N GROUP 1 VEHICLE CONTROL 0.52 0.12 14 2.62 0.34 14 3 1 14 2.21 0.44 14 0.60 0.18 14 40 3 14 6.05 0.52 14 8.3 1.8 .14 65.9 3.3 14 34.0 1.4 14 31.9 2.2 14 1.1 0.1 14 4.46 0.62 14 GROUP 2 TELOMER ALCOHOL 0.47 0.09 14 2.37 0.53 14 2 1 14 2.26 0.40 14 0.57 0.18 14 38 4 14 5.90 0.58 14 8.4 1.5 14 64.8 2.8 14 33.8 1.4 14 31.0 2.4 14 1.1 0.1 14 4.04 0.95 14 GROUP 3 TELOMER ACRYLATE GROUP 4 0.55 0.14 14 2.24 0.36 14 3 0 14 2.09 0.40 14 0.58 0.14 14 39 4 14 6.14 0.70 14 8.8 1.7 14 65.4 2.5 14 34.1 1.6 14 31.3 1.9 14 1.1 0.1 14 4.52 1.18 14 0.52 0.12 14 2.30 0.35 14 3 1 14 2.32 0.33 14 0.56 0.13 14 41 4 14 6.00 0.50 14 9.0 1.7 14 66.2 2.9 14 33.8 2.2 14 32.4 2.0 14 1.0 0.1 14 4.30 0.87 14 GROUP 5 PFHS GROUP 6 PFOS 0.57 0.09 14 2.45 0.69 14 2 1 14 1.81 0.28 14 0.51 0.16 14 40 3 14 6.02 0.72 14 8.5 0.9 14 65.4 4.1 14 33.7 1.5 14 31.7 3.1 14 1.1 0.1 14 4.06 1.10 14 0.63 0.32 14 2.32 0.38 14 3 1 14 2.04 0.35 . 14 0.54 0.16 14 41 4 14 5.98 1.02 14 10.2 1.9 14 66.8 3.1 14 34.9 1.7 14 31.8 2.4 14 1.1 0.1 14 4.30 1.14 14 */**: Ounnett-test based on pooled variance sig. at 5% or 1% level. +: Steel*test s1g. at 5% level. 91 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ "PFHS/PFOS `' BACK TO MAIN CHE-SUM - 4 09-N0V-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS FEMALES TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL INORG PHOSPH MEAN m m o 1/1 ST.DEV. N SODIUM nimol/1 MEAN ST.DEV. N POTASSIUM mmol/1 MEAN ST.DEV. N CALCIUM mmol/1 MEAN ST.DEV. N CHLORIDE mmol/1 MEAN ST.DEV. N ' 2.58 0.23 14 142.2 1.6 14 4.13 0.39 14 2.56 0.09 14 97 3 14 2.53 0.16 14 142.6 0.8 14 4.14 0.34 14 2.60 0.06 14 99 2 14 GROUP 3 TELOMER ACRYLATE GROUP 4 2.47 0.20 14 142.6 1.1 14 4.11 0.32 14 2.60 0.05 14 98 2 14 2.42 0.19 14 142.0 1.0 14 4.19 0.24 14 2.60 0.05 14 99 2 14 GROUP 5 PFHS 2.43 0.21 14 141.8 0.7 14 4.08 0.28 14 2.56 0.06 14 102 ** 2 14 GROUP 6 PFOS 2.59 0.23 14 142.5 1.1 14 4.06 0.30 14 2.60 0.07 14 101 2 4 /**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ *PFHS/PFOS " BACK TO MAIN CHE-SUM - 5 09-DEC-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT ALAT(GPT) ukat/1 ASAT(GOT) ukat/1 BILI T. umol/1 CHOLEST.T. mmol/I TRIGL. mmol/I CREATININE umol/1 GLUCOSE mmol/I UREA mmol/1 PROTEIN T. B/1 ALBUMIN g/1 GLOBULIN g/i A/G RATIO ALP ukat/1 MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N CAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 . TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS 0.86 0.19 3 2.80 0.55 3 3 0 3 1.95 0.50 3 0.53 0.09 3 35 8 3 5.65 0.39 3 6.8 1.3 3 55.9 1.5 3 . 29.0 1.0 3 26.9 2.1 3 1.1 0.1 3 6.59 2.07 3 0.62 0.08 3 2.70 0.40 3 3 1 3 1.67 0.69 3 0.61 0.20 3 34 2 3 6.23 1.66 3 7.3 0.5 3 51.5 * 1.0 3 28.1 0.5 3 23.4 0.8 3 1.2 0.0 3 6.93 1.02 3 0.65 0.12 3 2.91 0.03 3 3 1 3 1.57 0.04 3 0.89 0.35 3 33 2 3 6.85 0.93 3 6.9 0.7 3 57.6 0.9 3 29.5 0.3 3 28.1 0.8 3 1.0 0.0 3 5.82 1.22 3 0.85 0.18 3 2.86 0.59 3 3 1 3 1.93 0.43 3 0.70 0.10 3 39 2 3 8.33 * 0.11 3 6.8 0.5 3 53.6 3.2 3 27.8 1.3 3 25.8 2.1 3 1.1 0.1 3 5.94 1.02 3 0.81 0.13 3 2.43 0.42 3 2 0 3 1.38 0.22 3 0.65 0.09 3 39 4 3 7.14 0.16 3 6.7 0.8 3 54.7 0.7 3 28.9 1.0 3 25.8 0.9 3 1.1 0.1 3 6.72 0.80 3 0.64 0.17 3 1.92 0.48 3 3 1 3 1.27 0.19 3 0.50 0.10 3 39 9 3 6.22 1.02 3 7.4 0.7 3 56.5 0.7 3 2 9 .7 0.6 3 26.8 0.5 3 1.1 0.0 .3 5.61 1.21 3 */**: Dunnett test based on pooled variance s1g. at 5% or 1% level . +: Steel test s1g. at 5% level 93 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ */PFHS/PFOS " BACK TO MAIN CHE-SUM - 6 09-DEC-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY MALES , TREATMENT GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL INORG PHOSPH MEAN mmol/1 ST.DEV. N SODIUM mmol/1 MEAN ST.DEV. N POTASSIUM mmol/1 MEAN ST.DEV. N CALCIUM mmol/1 MEAN ST.DEV. N CHLORIDE mmol/1 MEAN ST.DEV. N 2.81 0.06 3 144.3 1.1 3 4.46 0.42 3 2.48 0.04 3 103 1 3 2.78 0.16 3' 143.4 2.1 3 4.51 0.23 3 2.44 0.06 3 102 3 3 GROUP 3 TELOMER ACRYLATE GROUP 4 2.74 0.23 3 142.0 0.8 3 4.95 0.13 3 2.48 0.03 3 100 2 3 2.64 0.11 3 141.1 * 0.7 3 4.41 0.20 3 2.50 0.11 3 100 1 3 GROUP 5 PFHS 2.51 0.14 3 142.4 0.9 3 4.56 0.15 3 2.45 0.04 3 102 2 3 GROUP 6 PFOS 2.65 0.12 3 142.8 0.1 3 4.56 0.24 3 2.57 0.07 3 103 2 3 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test slg. at 5% level. 94 BACK TO MAIN NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ '' PFHS/PFOS CHE-SUM - 7 0 9 -DEC-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT ALAT(GPT) ukat/1 ASAT(GOT) ukat/1 BILI T. UfROl/1 CHOLEST.T. mmol/l TRIGL. mmol/l CREATININE umol/1 GLUCOSE mmol/l UREA mmol/l PROTEIN T. 9/1 ALBUMIN g/i GLOBULIN gn A/G RATIO ALP ukat/1 MEAN ST.DEV. N MEAN ST.OEV. N MEAN ST.DEV. N MEAN ST.OEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 0.47 0.05 3 2.53 0.58 3 3 1 3 2.16 0.38 3 0.69 0.24 3 39. 2 3 7.34 1.68 3 6.8 0.7 3 63.6 2.8 3 32.2 2.7 3 31.5 1.2 3 1.0 0.1 3 3.30 0.12 3 GROUP 2 TELOMER ALCOHOL 0.63 0.34 3 2.61 0.69 3 2 1 3 1.92 0.43 3 0:43 0.03 3 40 2 3 6.52 0.66 3 7.3 1.4 3 61.7 3.8 3 3 2 .7 2.8 3 29.0 2.8 3 1.1 0.2 3 3.60 1.10 3 GROUP 3 TELOMER ACRYLATE GROUP 4 0.49 0.11 3 2.10 0.36 3 2 0 3 1.74 0.40 3 0.61 0.24 3 41 5 3 7.31 0.54 3 8.2 1.1 3 61.1 1.6 3 31.6 1.9 3 29.5 2.3 3 1.1 0.1 3 4.36 1.08 3 0.57 0.08 3 2.53 0.34 3 3 1 3 2.41 0.28 3 0.73 0.26 3 45 7 3 7.41 1.04 3 9.8 * 1.1 3 65.2 3.1 3 33.8 1.7 3 31.4 1.8 .3 1.1 0.0 3 3.85 0.88 3 GROUP 5 PFHS 0.52 0.12 3 1.66 0.15 3 3 0 3 2.11 0.40 3 0.40 0.09 3 44 4 3 7.55 1.70 3 7.6 0.4 3 66.4 4.2 3 31.4 1.0 3 35.1 3.3 3 0.9 0.1 3 3.27 1.20 3 GROUP 6 PFOS 0.45 0.07 3 2.09 0.17 3 2 1 3 2.02 0.37 3 0.31 0.08 3 42 1 3 7.69 0.33 3 8.0 1.2 3 60.6 5.2 3 32.5 1.2 3 28.1 4.0 3 1.2 0.1 3 4.32 1.51 3 */*: Dunnett -test based on pooled variance sig. at 5% or 1% level . +: Steel -test sig. at 5% level 95 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/' "" /PFHS/PFOS " BACK TO MAIN CHE-SUM - 8 09-DEC-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT INORG PHOSPH MEAN mmol/1 ST.DEV. N SODIUM m m o 1/1 MEAN ST.DEV. N POTASSIUM mmol/I MEAN ST.DEV. N CALCIUM mmol/1 MEAN ST.DEV. N CHLORIDE mmol/1 MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 2.47 0.09 3 142.4 1.0 3 4.08 0.09 3 2.63 0.14 3 99 1 3 GROUP 2 TELOMER ALCOHOL 2.57 0.36 3 142.8 1.3 3 3.96 0.20 3 2.59 0.06 3 99 1 3 GROUP 3 TELOMER ACRYLATE GROUP 4 2.32 0.06 3 142.0 1.0 3 4.05 0.42 3 2.57 0.09 3 99 4 3 2.55 0.27 3 141.3 1.5 3 4.34 0.38 3 2.68 0.03 3 98 2 3 GROUP 5 PFHS 2.42 0.26 3 141.6 0.8 3 3.90 0.23 3 2.68 0.14 3 98 3 3 GROUP 6 PFOS 2.29 0.10 3 142.5 0.8 3 4.11 0.15 3 2.63 0.07 3 100 2 3 */*: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level. 96 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS BACK TO MAIN CHE-SUM - 9 09-N0V-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT ALAT(GPT) MEAN ukat/1 ST.DEV. N ASAT(GOT) ukat/1 MEAN ST.DEV. N Bill T. umo1/1 MEAN ST.DEV. N CHOLEST.T. mmol/1 MEAN ST.DEV. N TRIGL. mrnol/1 MEAN ST.DEV. N CREATININE umol/1 MEAN ST.DEV. N GLUCOSE nnnol/1 MEAN ST.DEV. N UREA mmol/I MEAN ST.DEV. N PROTEIN T. 0/1 MEAN ST.DEV. N ALBUMIN g/1 MEAN ST.DEV. N GLOBULIN g/1 MEAN ST.DEV. N A/G RATIO MEAN ST.DEV. N ALP ukat/1 MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL 0.59 0.17 3 2.65 0.25 3 3 1 3 1.94 0.18 3 0.76 0.39 3 36 1 3 8.01 0.40 3 6.3 0.3 3 62.2 0.1 3 28.6 0.6 3 33.5 0.6 3 0.9 0.0 3 4.33 0.65 3 0.68 0.07 3 2.44 0.25 3 2 1 3 1.25 0.13 3. 0.58 0.03 3 33 4 3 7.45 1.37 3 6.3 0.2 3 55.9 * 1.8 3 2 7.3 0.5 3 28.6 2.3 3 1.0 . 0.1 3 6.20 1.91 3 GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS 0.60 0.03 3 2.06 0.24 3 3 1 3 1.53 0.39 3 0.53 0.11 3 34 4 3 7.06 1.05 3 6.6 0.9 3 55.4 3.5 3 2 7.0 1.4 3 28.4 2.3 3 1.0 0.0 3 5.28 1.46 3 0.58 0.17 3 2.39 0.50 3 3 1 3 1.66 0.35 3 0.56 0.02 3 38 1 3 6.12 0.55 3 7.5 0.7 3 57.0 * 2.3 3 2 7 .7 1.3 3 29.3 1.8 3 0.9 0.1 3 5.43 1.31 3 0.75 0.17 3 2.40 0.20 3 2 1 3 1.24 0.09 3 0.36 0.19 3 43 6 3 5.57 * 0.70 3 8.3 2.0 3 57.3 1.2 3 2 7.3 0.6 3 30.0 1.0 3 0.9 0.0 3 6.00 1.94 3 GROUP 6 PFOS 0.64 0.12 3 1.87 * 0.13 3 2* 0 3 1.34 0.37 3 0.45 0.12 3 32 4 3 6.70 0.62 3 6.0 0.5 3 54.5 ** 2.5 3 2 7 .5 0.7 3 26.9 2.0 3 1.0 0.1 3 4.73 0.19 3 */**: Dunnett -test based on pooled variance s1g. at 5% or 1% level. +: Steel-test si g. at 5% level 97 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS " BACK TO MAIN CHE-SUM -10 09-N0V-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT INORG PHOSPH MEAN m m o 1/1 ST.DEV. N SODIUM mmol/1 MEAN ST.DEV. N POTASSIUM mmol/1 MEAN ST.DEV. N CALCIUM mmol/1 MEAN ST.DEV. N CHLORIDE mmol/1 MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 3.02 0.13 3 141.6 0.9 3 5.33 0.21 3 2.58 0.02 3 102 2 3 GROUP 2 TELOMER ALCOHOL 2.64 0.28 3 142.9 1.3 3 4.59 * 0.14 3 2.48 * 0.06 3 103 3 3 GROUP 3 TELOMER ACRYLATE GROUP 4 2.64 0.24 3 142.5 1.2 3 4.64 * 0.33 3 2.48 * 0.05 3 104 2 3 2.46 * 0.18 3 142.0 0.6 3 4.55 ** 0.12 3 2.46 * 0.03 3 104 1 3 GROUP 5 PFHS 2.65 0.18 3 143.8 1.0 3 4.57 * 0.42 3 2.49 0.02 3 103 1 3 GROUP PFOS 2.59 0.20 3 142.7 0.2 3 4.65 ' 0.11 3 2.48 ' 0.05 3 107 * 2 3 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level. 98 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ *~ I P FHS/PFOS " BACK TO MAIN CHE-SUM -11 09-N0V-98 CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT ALAT(GPT) ukat/1 ASAT(GOT) ukat/1 BILI T. umol/1 CHOLEST.T. mmol/I TRIGL. mmol/I CREATININE umol/1 GLUCOSE mmol/1 UREA mmol/1 PROTEIN T. g/i ALBUMIN g/1 nGLOBULIN g A/G RATIO ALP ukat/1 MEAN ST.DEV. . N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS GROUP 6 PFOS 0.38 0.09 3 2.25 0.31 3 2 1 3 2.50 0.38 3 0.63 0.13 3 41 3 3 7.16 1.26 3 7.5 1.6 3 64.9 4.1 3 31.3 1.7 3 33.6 2.5 3 0.9 0.0 3 2.95 0.46 3 0.37 0.04 2' 2.31 0.33 3 2 1 3 2.41 0.36 3 0.74 0.27 3 42 5 3 6.43 0.47 3 7.5 1.3 3 64.3 2.5 3 31.6 1.2 3 32.6 1.9 3 1.0 0.1 3 3.03 1.24 3 0.46 . 0.12 3 1.89 0.45 3 3 1 3 2.30 0.11 3 0.47 0.04 3 42 3 3 6.90 0.37 3 8.0 1.0 3 67.7 2.2 3 33.4 3.4 3 34.4 1.5 3 1.0 0.1 3 3.11 0.92 3 0.63 0.31 3 2.45 0.26 3 2 0 3 2.51 0.49 3 0.51 0.18 3 . 44 4 3 6.34 1.56 3 8.3 1.5 3 65.3 3.4 3 30.6 1.3 3 34.7 2.1 3 0.9 0.0 3 2.93 0.85 3 0.47 0.16 3 2.52 0.49 3 2 0 3 2.06 0.35 3 0.40 0.06 3 39 1 3 6.86 0.41 3 7.0 0.3 3 69.4 0.9 3 34.2 0.7 3 35.2 1.4 3 1.0 0.1 3 2.70 0.85 3 0.50 0.02 3 1.92 0.05 3 2 1 3 2.13 0.22 3 0.50 0.21 3 39 1 3 7.65 1.09 3 10.7 3.7 3 65.3 5.4 3 32.8 2.0 3 32.5 3.3 3 1.0 0.0 3 3.38 0.19 3 */**: Dunnett -test based on pooled variance s1g. at 5% or 1% level. +: Steel-test sig. at 5% level 99 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ *PFHS/PFOS BACK TO MAIN CHE-SUM -12 O9-N0V-98 CLINICAL BIOCHEMISTRY S U M M A R Y AFTER 4 WEEKS RECOVERY FEMALES TREATMENT IN0R6 PHOSPH MEAN mmol/I ST.DEV. N SODIUM mmol/I MEAN ST.DEV. N POTASSIUM mmol/I MEAN ST.DEV. N CALCIUM mmol/I MEAN ST.DEV. N CHLORIDE. mmol/I MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 2.33 0.17 3 142.3 1.8 3 4.12 0.34 3 2.52 0.05 3 100 4 3 GROUP 2 TELOMER ALCOHOL 2'24 0.12 3 141.6 0.5 3 4.14 0.29 3 2.53 0.10 3 101 3 3 GROUP 3 TELOMER ACRYLATE GROUP 4 1.98 0.20 3 140.7 1.7 3 4.48 0.89 3 2.55 0.07 3 100 4 3 2.22 0.30 3 141.3 0.7 3 4.26 0.51 3 2.57 0.04 3 99 3 3 GROUP 5 PFHS GROUP 6 PFOS 2.16 0.08 3 141.0 1.4 3 4.02 0.33 3 2.60 0.01 3 102 3 3 2.27 0.26 3 141.1 1.6 3 4.16 0.18 3 2.64 0.03 3 103 5 3 */'*: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test sig. at 5% level. 1 00 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ '' PFHS/PFOS " BACK TO MAIN OW-SUM - l 08-DEC-98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS MALES TREATMENT BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN TESTES MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL GROUP 2 TELOMER ALCOHOL 315 315 30 16 8 8' 2.01 0.07 8 1.96 0.07 8 1.133 1.134 0.108 0.144 88 10.05 1.24 8 10.35 0.68 8 2.30 0.36 8 2.34 0.18 8 0.045 0.006 8 0.053 0.008 8 0.580 0.119 8 0.566 0.069 8 2.87 0.23 8 3.05 0.22 8 GROUP 3 TELOMER ACRYLATE GROUP 4 305 13 8 1.95 0.06 8 1.097 0.115 8 9.36 0.60 8 2.20 0.15 8 0.052 0.007 8 0.567 0.065 8 2.78 0.15 8 306 27 8 2.00 0.06 8 1.154 0.106 8 9.04 1.08 8 2.16 0.25 8 0.046 0.008 8 0.578 0.075 8 2.86 0.19 8 GROUP 5 PFHS GROUP 6 PFOS 308 28 8 1.96 0.09 8 1.107 0.088 8 13.78 ** 1.83 8 2.34 0.22 8 0.050 0.009 8 0.570 0.076 8 2.80 0.80 8 318 25 8 2.00 0.05 8 - 1.131 0.103 8 11.79 * 1.00 8 2.19 0.18 8 0.046 0.013 8 0.558 0.096 8 2.85 0.18 8 */**: Dunnett-test based on pooled variance sig. at 5% or 1% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ 'PFHS/PFOS " BACK TO MAIN OW-SUM - 2 08-DEC-98 ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS MALES TREATMENT BODY W. (GRAM) BRAIN (%) HEART (%) LIVER (%) KIDNEYS (%) ADRENALS (%) SPLEEN (%) TESTES (%) MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST. DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 315 30 8 0.64 0.05 8 0.362 0.037 a 3.19 0.21 8 0.73 0.09 8 0.014 0.002 8 0.183 0.027 8 0.91 0.05 8 GROUP 2 TELOMER ALCOHOL 315 16 8 0.62 0.03 8 0.361 0.046 8 3.30 0.29 8 0.74 0.06 8 0.017 0.003 8 0.180 0.017 8 0.97 0.09 8 GROUP 3 TELOMER ACRYLATE GROUP 4 305 13 8 0.64 0.03 8 0.360 0.037 8 3.07 0.11 8 0.72 0.05 8 0.017 0.002 8 0.186 0.015 8 0.91 0.06 8 306 27 8. 0.66 0.05 8 0.378 0.026 8 2.95 0.14 8 0.70 0.03 8 0.015 0.003 8 0.189 0.018 8 0.94 0.12 8 GROUP 5 PFHS 308 28 8 0.64 0.05 8 0.36a 0.023 8 4.47 * 0.40 8 0.76 0.05 8 0.016 0.003 8 0.185 0.016 8 0.90 0.24 8 GROUP 6 PFOS 318 25 8 0.63 0.05 8 0.357 0.041 8 3.71 ** 0.30 8 0.69 0.07 8 0.014 0.003 8 0.175 0.026 8 0.90 0.05 8 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. 1 02 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ *. PFHS/PFOS BACK TO MAIN OW-SUM - 3 0 8 -DEC-98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS FEMALES TREATMENT BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN OVARIES MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 - VEHICLE CONTROL 214 22 8 1.88 0.08 8 0.852 0.054 8 6.62 0.50 8 1.50 0.15 8 0.060 0.009 8 0.423 0.051 8 0.124 0.023 8 GROUP 2 TELOMER ALCOHOL 211 16 8' 1.93 0.08 8 0.813 0.054 8 7.18 0.55 8 1.63 0.15 8 0.064 0.009 8 0.450 0.037 8 0.133 0.020 8 GROUP 3 TELOMER ACRYLATE GROUP 4 200 16 8 1.85 0.05 7 0.790 0.071 7 6.37 0.47 7 1.51 0.11 7 0.060 0.009 7 0.417 0.086 7 0.135 0.015 7 208 15 8 1.90 0.08 8 0.805 0.073 8 6.53 0.72 8 1.53 0.17 8 0.070 0.037 8 0.608 0.373 8 0.124 0.030 8 GROUP 5 PFHS 201 13 8 1.88 0.09 8 0.806 0.083 8 6.71 0.70 8 1.51 0.12 8 0.058 0.009 8 0.407 0.033 8 0.132 0.018 8 GROUP 6 PFOS 187 * 16 8 1.88 0.05 8 0.748 * 0.073 8 7.80 ** 0.88 8 1.46 0.12 8 0.054 0.006 8 0.412 0.046 8 0.128 0.019 8 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. NOTOX Project 242933 '- TELOMER ALCOHOL/TELOMER ACRYLATE/ ... PFHS/PFOS " BACK TO MAIN OW-SUM - 4 08-DEC-98 ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS FEMALES TREATMENT BODY W. (GRAM) BRAIN (%) HEART (%) LIVER (%) KIDNEYS (%) ADRENALS (%) SPLEEN (%) OVARIES (%) MEAN ST.DEV. N MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST.DEV. N. MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 214 22 8 0.89 0.08 8 0.401 0.035 8 3.11 0.19 8 0.70 0.03 8 0.028 0.006 8 0.198 0.024 8 0.058 0.010 8 GROUP 2 TELOMER ALCOHOL GROUP 3 TELOMER ACRYLATE GROUP 4 GROUP 5 PFHS 211 16 8 0.92 0.05 8 0.385 0.027 8 3.40 0.13 B 0.77 0.07 8 0.031 0.005 8 0.214 0.020 8 0.063 0.012 8 200 16 8 0.93 0.06 .7 0.395 0.023 7 3.19 0.13 7 0.76 0.04 7 0.030 0.002 7 0.208 0.037 7 0.068 0.008 7 208 15 8 0.91 0.04 8 0.388 0.030 8 3.14 0.28 8 0.74 0.08 8 0.033 0.016 8 0.298 0.201 8 0.060 0.015 8 201 13 8 0.94 0.05 .8 0.402 0.034 8 3.35 0.35 8 0.75 0.06 8 0.029 0.005 8 0.204 0.016 8 0.066 0.008 8 GROUP 6 PFOS 187 * 16 8 1.01 0.07 8 0.399 0.028 8 4.15 ` 0.18 8 0.78 * 0.03 8 0.029 0.003 8 0.220 0.013 8 0.069 0.013 8 /**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. NOTOX Project 242933 '" TELOMER ALCOHOL/TELOMER ACRYLATE/ .,/PFHS/PFOS BACK TO MAIN OW-SUM - 5 08-DEC-98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN TESTES MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 320 37 3 1.95 0.12 3 1.091 0.117 3 8.24 0.78 3 2.04 0.31 3 0.042 0.007 3 0.441 0.084 3 2.81 0.24 3 GROUP 2 TELOMER ALCOHOL 314 17 3 1.98 0.07 3 1.062 0.053 3 8.27 0.63 3 2.08 0.03 3 0.039 0.008 3 0.568 0.012 3 2.13 0.89 3 GROUP 3 TELOMER ACRYLATE GROUP 4 344 31 3 1.95 0.13 3 1.172 0.097 3 9.89 1.91 3 2.41 0.31 3 0.043 0.006 3 0.543 0.037 3 3.09 0.16 3 366 44 3 2.02 0.11 3 1.153 0.027 3 10.30 1.19 3 2.39 0.25 3 0.055 0.012 3 0.623 0.102 3 3.35 0.17 3 GROUP 5 PFHS 324 69 3 1.93 0.18 3 1.149 0.145 3 9.55 2.63 3 2.18 0.51 3 0.041 0.005 3 0.541 0.080 3 2.61 1.09 3 GROUP 6 PFQS 380 12 3 2.03 0.03 3 1.299 0.054 3 14.07 0.63 3 2.65 0.06 3 0.048 0.007 3 0.635 0.121 3 3.03 0.26 3 /**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ '/PFHS/PFOS `' BACK TO MAIN OW-SUM - 6 08-DEC-98 ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 2 WEEKS RECOVERY MALES TREATMENT BODY W. (GRAM) BRAIN (%) HEART (%) LIVER (%) KIDNEYS (%) ADRENALS (%) SPLEEN (%) TESTES {%) MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 320 37 3 0.61 0.04 3 0.342 0.007 3 2.58 0.11 3 0.64 0.06 3 0.013 0.002 3 0.138 0.016 3' 0.88 0.04 3 GROUP 2 TELOMER ALCOHOL 314 17 3' 0.63 0.04 3 0.339 0.029 3 2.63 0.07 3 0.66 0.05 3 0.013 0.003 3 0.181 0.011 3 0.68 0.30 3 GROUP 3 TELOMER ACRYLATE GROUP a 344 31 3 0.57 0.06 3 0.344 0.055 3 2.86 0.30 3 0.70 0.03 3 0.013 . 0.001 3 0.159 0.024 3 0.90 0.09 3 366 44 3 0.55 0.04 3 0.318 0.042 3 2.82 0.12 3 0.65 . 0.05 3 0.016 0.005 3 0.170 0.007 3 0.92 0.06 3 GROUP 5 PFHS 324 69 3 0.61 0.09 3 0.361 0.045 3 2.93 0.20 3 0.67 0.04 3 0.013 0.001 3 0.169 0.019 3 0.78 0.22 3 GROUP 6 PFOS 380 12 3 0.53 0.02 3 0.342 0.009 3 3.70 ** 0.22 3 0.70 0.02 3 0.013 0.002 3 0.167 0.028 3 0.80 0.04 3 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. 1 06 NOTOX Project 242933 *- TELOMER ALCOHOL/TELOMER ACRYLATE/ ..... PFHS/PFOS " BACK TO MAIN OW-SUM - 7 08-DEC-98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN OVARIES MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 239 29 3 1.96 0.10 3 0.898 0.053 3 7.69 1.33 3 1.54 0.16 3 0.055 0.003 3 0.420 0.042 3 0.285 0.250 3 GROUP 2 TELOMER ALCOHOL 211 17 3 1.89 0.07 3 0.858 0.052 3 6.82 0.86 3 1.51 0.17 3 0.063 0.010 3 0.424 0.097 3 0.130 0.005 3 GROUP 3 TELOMER ACRYLATE GROUP 4 . GROUP 5 PFHS 202 14 3 1.91 0.03 3 0.831 0.056 3 6.38 0.91 3 1.52 0.16 3 0.065 0.017 3 0.416 0.094 3 0.126 0.010 3 217 28 3 1.91 0.03 3 0.936 0.052 3 7.07 0.09 3 1.60 0.12 3 0.069 0.005 3 0.414 0.016 3 0.145 0.020 3 224 11 3 1.92 0.02 3 0.840 0.054 3 7.19 0.67 3 1.51 0.06 3 0.058 0.003 3 0.449 0.077 3 0.133 0.015 3 GROUP 6 PFOS 200 3 3 1.99 0.06 3 0.723 ** 0.018 3 7.55 0.56 3 1.38 0.11 3 0.056 0.001 3 0.392 0.025 3 0.132 0.006 3 /**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. BACK TO MAIN NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE, "PFHS/PFOS " OW-SUM - 8 08-DEC-98 ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 2 WEEKS RECOVERY FEMALES TREATMENT BODY W. (GRAM) MEAN ST.DEV. N BRAIN (%> ' MEAN ST.DEV. N HEART (%) MEAN ST.DEV. N LIVER (%) MEAN ST.DEV. N KIDNEYS (%) MEAN ST.DEV. N ADRENALS (%) MEAN ST.DEV. N SPLEEN (*) MEAN ST.DEV. N OVARIES l*) MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 239 29 3 0.83 0.08 3 0.378 0.024 3 3.21 0.18 3 0.65 0.04 3 0.023 0.002 3 0.177 0.026 3 0.113 0.085 3 GROUP 2 TELOMER ALCOHOL 211 17 3' 0.90 0.04 3 0.408 0.042 3 3.22 0.23 3 0.72 0.08 3 0.030 0.006 3 0.199 0.028 3 0.062 0.005 3 GROUP 3 TELOMER ACRYLATE GROUP 4 202 14 3 0.95 0.07 3 0.412 0.013 3 3.15 0.23 3 0.75 0.03 3 0.032 0.006 3 0.205 0.035 3 0.063 0.007 3 . 217 28 3 0.89 0.10 3 0.435 0.042 3 3.30 0.45 3 0.74 0.04 3 0.032 0.005 3 0.192 0.018 3 0.067 0.003 3 GROUP 5 PFHS 224 11 3 0.86 0.04 3 0.375 0.008 3 3.21 0.23 3 0.68 0.06 3 0.026 0.001 3 0.202 0.041 3 0.059 0.004 3 GROUP 6 PFOS 200 3 3 1.00 0.03 3 0.362 0.007 3 3.78 0.32 3 0.69 0.06 3 0.028 0.001 3 0.197 0.015 3 0.066 0.004 3 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. 1 08 NOTOX Project 242933 '- TELOMER ALCOHOL/TELOMER ACRYLATE/ .'PFHS/PFOS " BACK TO MAIN OW-SUM - 9 08-DEC-98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN TESTES MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST. DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 389 28 3 1.97 0.15 3 1.196 0.093 3 10.77 1.37 3 2.44 0.23 3 0.046 0.011 3 0.614 0.040 3 2.86 0.14 3 GROUP 2 TELOMER ALCOHOL 363 33 3' 2.05 0.04 3 1.207 0.018 3 9.78 0.44 3 2.42 0.06 3 0.050 0.009 3 0.540 0.082 3 3.19 0.43 3 GROUP 3 TELOMER ACRYLATE GROUP 4 378 3 3 2.09 0.03 3 1.217 0.077 3 10.30 0.69 3 2.51 0.04 3 0.054 0.004 3 0.591 0.061 3 3.11 0.06 3 401 36 3 2.11 0.19 3 1.299 0.147 3 10.06 0.35 3 2.59 0.24 3 0.056 0.013 3 0.720 0.037 3 3.10 0.22 3 GROUP 5 PFHS 335 20 3 2.02 0.08 3 1.048 0.153 3 8.84 0.63 3 2.24 0.41 3 0.047 0.003 3 0.552 0.065 3 3.09 0.11 3 GROUP 6 PFOS 344 38 3 2.00 0.07 3 1.231 0.248 3 11.17 1.94 3 2.48 0.21 3 0.046 0.009 3 0.629 0.136 3 3.27 0.30 3 */**: Ounnett-test based on pooled variance s1g. at 5% or 1% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ */PFHS/PFOS " BACK TO MAIN OW-SUM - 10 08-DEC-98 ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS RECOVERY MALES TREATMENT GROUP 1 VEHICLE CONTROL BODY W. (GRAM) BRAIN (%) HEART (%) LIVER (%) KIDNEYS (%) ADRENALS (%) SPLEEN (%) TESTES (%) MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N 389 28 3 0.51 0.03 3 0.308 0.027 3 2.76 0.17 3 0.63 0.05 3 0.012 0.003 3 0.159 0.017 3 0.74 0.04 3 GROUP 2 TELOMER ALCOHOL 363 33 3 0.57 0.04 3 0.335 0.030 3 2.70 0.13 3 0.67 0.04 3 0.014 0.002 3 0.149 0.019 3 0.88 0.14 3 GROUP 3 TELOMER ACRYLATE GROUP 4 378 3 3 0.55 0.01 3 0.322 0.018 3 2.72 0.18 3 0.66 0.01 3 0.014 0.001 3 0.156 0.016 3 0.82 0.02 3 401 36 3 0.53 0.03 3 0.324 0.016 3 2.52 0.21 3 0.65 0.08 3 0.014 0.002 3 0.181 0.026 3 0.78 0.05 3 GROUP 5 PFHS 335 20 3 0.60 * 0.01 3 0.313 0.042 3 2.64 0.03 3 0.67 0.14 3 0.014 0.000 3 0.164 0.010 3 0.92 * 0.02 3 GROUP 6 PFOS 344 38 3 0.59 0.05 3 0.356 0.035 3 3.23 * 0.22 3 0.72 0.02 3 0.014 0.004 3 0.182 0.022 3 0.95 * 0.08 3 */**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. 110 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS '' BACK TO MAIN OW-SUM - 11 08-DEC-98 ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN OVARIES MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV-. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 243 27 3 1.91 0.09 3 0.916 0.118 3 7.01 1.14 3 1.60 0.25 3 0.065 0.012 3 0.445 0.056 3 0.133 0.013 3 GROUP 2 TELOMER ALCOHOL 228 11 3' 1.97 0.06 3 0.851 0.109 3 6.87 0.57 3 1.63 0.09 3 0.064 0.013 3 0.443 0.140 3 0.146 0.029 3 GROUP 3 TELOMER ACRYLATE GROUP 4 226 25 3 1.88 0.06 3 0.870 0.046 3 6.85 0.86 3 1.54 0.14 3 0.062 0.008 3 0.426 0.065 3 0.145 0.013 3 235 35 3 1.96 0.05 3 0.918 0.092 3 7.30 1.56 3 1.68 0.25 3 0.061 0.001 3 0.504 0.104 3 0.165 0.040 3 GROUP 5 PFHS 226 13 3 1.88 0.08 3 0.860 0.065 3 6.83 0.20 3 1.62 0.12 3 0.065 0.003 3 0.355 0.024 3 0.153 0.047 3 GROUP 6 PFOS 208 11 3 1.88 0.09 3 0.825 0.028 3 7.49 0.30 3 1.68 0.12 3 0.058 0.010 3 0.427 0.060 3 0.141 0.003 3 /**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. NOTOX Project 242933 *- TELOMER ALCOHOL/TELOMER ACRYLATE/ .... PFHS/PFOS BACK TO MAIN OW-SUM - 12 0 8 -DEC-98 ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS RECOVERY FEMALES TREATMENT BODY W. (GRAM) BRAIN (%) HEART (*) LIVER (%) KIDNEYS (%) ADRENALS (%) SPLEEN (%) OVARIES (%) MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N MEAN ST.DEV. N GROUP 1 VEHICLE CONTROL 243 27 3 0.79 0.08 3 0.379 0.039 3 2.88 0.20 3 0.66 0.04 3 0.027 0.005 3 0.184 0.023 3 0.055 0.009 3 GROUP 2 TELOMER ALCOHOL 228 11 3 0.86 0.03 3 0.372 0.029 3 3.02 0.21 3 0.72 0.07 3 0.028 0.006 3 0.193 0.052 3 0.064 0.010 3 GROUP 3 TELOMER ACRYLATE GROUP 4 226 25 3 0.84 0.06 3 0.387 0.021 3 3.03 0.14 3 0.69 0.07 3 0.028 0.004 3 0.192 0.046 3 0.065 0.011 3 235 35 3 0.85 0.12 3 0.392 0.021 3 3.09 0.29 3 0.71 0.05 3 0.026 0.003 3 0.216 0.048 3 0.070 0.012 3 GROUP 5 PFHS GROUP 6 PFOS 226 13 3 0.83 0.01 3 0.380 0.017 3 3.02 0.17 3 0.72 0.08 3 0.029 0.001 3 0.157 0.019 3 0.068 0.023 3 208 11 3 0.90 0.04 3 0.398 .0.032 .3 3.61 ** 0.20 3 0.81 * 0.03 3 0.028 0.006 3 0.206 0.031 3 0.068 0.002 3 /*: Dunnett-test based on pooled variance s1g. at 5% or 1% level. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ HISTORICAL DATA HAEMATOLOGY 1 ANIMALS OF 10*12 WEEKS OF AGE MALES AND FEMALES PFHS/PFOS PARAMETER SEX N MEAN ST. DEV. MIN. RBC M T/l F HB mmol/1 M F HCT M 1/1 F MCV fl M F MCH fmol Mriir mmol/l M F M F PLATELETS M G/l F RDW M %F WBC M G/l F SEG l(rel) M F EO l(rel) M F BASO l(rel) M F LYMPH l(rel) M F MONO l(rel) M F PT M sec F PTT M sec F 50 44 50 44 ' 50 44 50 44 50 44 50 44 50 44 50 44 50 44 50 44 50 44 50 44 50 44 50 44 44 37 44 36 7.08 6.63 9.8 9.5 0.415 0.386 58.6 58.3 1.387 1.441 23.7 24.7 1137 1114 12.2 11.7 11.8 9.2 0.11 0.097 0.006 0.008 0 0 0.855 0.87 0.028 0.025 13.2 13.9 16.5 15.5 0.36 0.32 6.42 6 0.5 8.8 0.3 8.9 0.019 . 0.384 0.016 0.353 1.8 55 1.4 55.4 0.073 0.055 1.245 1.345 1.1 21.1 0.6 23.5 148 760 139 753 0.7 11.1 0.6 10.6 2.7 4.7 2.6 5.3 0.049 0.065 0.015 0.02 0.007 0.008 0 0 00 00 0.061 0.07 0.705 0.705 0.021 0.020 0 0 1 11.2 0.9 12 1.8 11.9 1.9 10.3 . MAX. 7.95 7.5 10.8 10.4 0.463 0.437 63.6 60.7 1.52 1.584 25.5 26.3 1486 1513 14.4 13.1 17.5 18.7 0.235 0.27 0.02 0.03 0 0 0.985 0.965 0.085 0.075 15.5 15.7 19.7 19.4 BACK TO MAIN HIS-SUM - 1 22-APR-99 1 Data are from Sprague Oawley control rats which received the vehicle (in general 0.9% NaCl) via continuous Intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated. 113 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ HISTORICAL DATA HAEMATOLOGY1 ANIMALS OF 13-18 WEEKS OF AGE MALES AND FEMALES PFHS/PFOS PARAMETER SEX N MEAN ST. DEV. MIN. RBC M T/l F HB mmol/1 M F HCT M 1/1 F MCV M fl F MCH fmol M F MCHC mmol/1 M F PLATELETS M G/l F RDW M %F WBC M G/l F SEG l(rel) M F EO l(rel) M F BASO l(rel) LYMPH l(rel) M F M F MONO l(rel) M F PT M sec F PTT M sec F 42 30 42 30 42 30 42 30 42 30 42 30 42 30 42 30 42 30 42 30 42 30 42 30 42 30 42 30 38 29 38 29 7.34 6.81 9.3 8.8 0.399 0.376 54 55.2 1.251 1.3 23.1 23.6 1096 1130 14.6 12.9 14.2 8.4 0.157 0.148 0.009 0.008 0 0 0.803 0.823 0.031 0.020 14.2 13.8 16.4 16.3 0.73 0.47 1.3 0.6 0.047 0.027 2.9 2.1 0.087 0.054 0.8 0.5 170 145 1.8 0.8 5.6 2 0.093 0.102 0.009 0.008 0 0 0.108 0.105 0.025 0.016 1.3 1 2.1 1.9 5.59 5.59 5.6 7 0.264 0.313 45.3 51.5 0.961 1.19 21.2 22.4 669 855 12.7 11.6 6.1 4.7 0.04 0.04 0 0 0 0 0.45 0.54 0 0 11.9 11.9 11.6 12.3 MAX. 8.7 7.65 11 9.9 0.472 0.423 59.6 59.8 1.391 1.42 24.4 24.6 1455 1515 20.5 15.2 33.9 12 0.505 0.42 0.035 0.03 0 0 0.935 0.945 0.105 0.065 17.3 15.6 21.2 19.6 BACK TO MAIN HIS-SUM - 2 22-APR-99 1 Data are from Sprague Dawley control rats which received the vehicle (1n general 0.9% NaCl) via continuous intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated. 114 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE PFHS/PFOS HISTORICAL DATA CLINICAL BIOCHEMISTRY1 ANIMALS OF 10-12 WEEKS OF AGE MALES AND FEMALES . PARAMETER SEX N MEAN ST. DEV. MIN. ALAT/GPT jikat/1 ASAT/GOT pleat/1 BILI T. pmol/1 CHOLEST. T. mmol/1 TRIGL. mmol/1 CREATININE pmol/1 GLUCOSE mmol/1 UREA mraol/1 PROTEIN T. 0/1 ALBUMIN o/i GLOBULIN 8/1 A/G RATIO 1 ALP pkat/1 SODIUM mmol/1 POTASSIUM mmol/1 CHLORIDE mmol/1 M F M F M F M F M F M F M F M F M F M F M F M F M F M F M F M F 51 45 51 45 51 45 51 45 51 45 51 45 51 45 51 45 51 45 51 45 51 45 51 45 51 45 51 51 45 51 45 0.66 0.54 2.51 2.46 2 3 1.95 2.05 0.69 0.42 33 40 6.03 5.32 6.1 8.1 64.1 67.4 29.5 32.6 34.6 34.8 0.9 1 7.38 4.3 143.3 141.2 4.83 4.59 100 101 0.11 0.15 0.6 0.43 1 1 0.34 . 0.4 0.37 0.09 6 6 1.07 0.68 1.6 2.1 2.9 4.2 1.8 2 2.5 4.5 0.1 0.1 1.87 1.19 1.6 1.6 0.43 0.3 3 3 0.45 0.24 1.54 1.69 1 2 1.25 1.08 0.34 0.26 25 31 3.86 3.87 3 4.5 57.7 59.5 24.5 28.4 29.1 25.8 0.6 0.7 2.94 2.63 138.5 137.5 3.35 3.97 95 94 . MAX. 0.98 1.06 3.8 3.48 4 5 2.98 3.31 2.11 0.68 54 56 8.45 7.4 8.9 12.8 70.9 75.5 32.8 36.6 40.9 43.7 1 1.3 12.3 7.86 146.3 144.8 5.97 5.32 106 110 BACK TO MAIN HIS-SUM - 3 22-APR-99 1 Data are from Sprague Dawley control rats which received the vehicle (1n general 0.9% NaCl) via continuous intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated. NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE PFHS/PFOS HISTORICAL DATA CLINICAL BIOCHEMISTRY1 ANIMALS OF 10-12 WEEKS OF AGE MALES AND FEMALES PARAMETER SEX N CALCIUM mmol/I M F INORG. PHOSPH. M mmol/1 F 51 45 51 45 MEAN 2.6 2.67 2.78 2.55 ST. DEV. MIN. 0.09 0.09 .21 0.25 2.41 2.46 2.29 2.09 . MAX. 2.8 2.86 3.27 3.38 BACK TO MAIN HIS-SUM - 4 2 2 -APR-99 1 Data are from Sprague Dawley control rats which received the vehicle (1n general 0.9% NaCl) via continuous Intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated. 116 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS HISTORICAL DATA CLINICAL BIOCHEMISTRY1 ANIMALS OF 13-18 WEEKS OF AGE MALES AND FEMALES PARAMETER SEX N ALAT/GPT pkat/1 M F 42 28 ASAT/GOT pkat/1 M F 42 28 BILI T. pmol/1 M 42 F 28 CHOLEST. T. mmol/1 M F 42 28 TRIGL. mmol/1 M 42 F 28 CREATININE Mmol/1 M 41 F 28 GLUCOSE mmol/1 M 42 F 28 UREA mmol/1 M 46 F 32 PROTEIN T. 9/1 ALBUMIN 9/1 GLOBULIN 9/1 A/G RATIO 1 M F M F M F M F 42 28 42 28 42 28 42 28 ALP pkat/1 M 42 F 28 SODIUM mmol/1 M 42 F 28 POTASSIUM mmol/1 M F 42 . 28 CHLORIDE mmol/1 M F 42 28 MEAN ST. DEV. MIN. 0.77 0.64 2.64 2.31 2 3 1.93 2.49 0.58 0.47 37 42 6.38 5.93 5.5 5.7 66,4 72.5 28.1 32 38.2 40.5 0.7 0.8 4.21 3.32 142 140 5 4.43 101 103 0.38 0.19 0.55 0.42 1 1 0.43 0.45 0.25 0.19 4 5 1.22 0.71 1.4 1.5 3.8 3.5 3 3.8 4.2 4.4 0.1 0.2 1.04 2.51 2.4 2.7 0.36 0.29 3 3 0.47 0.33 1.65 1.45 1 2 0.98 1.69 0.23 0.27 27 35 4.21 5.04 3 3.7 56 66.8 21 23.9 31 34.4 0.4 0.5 2.45 1.15 137.1 135.8 4.38 3.87 96 98 MAX. 2.98 1.15 4.05 3.09 4 5 2.8 3.27 1.13 1.24 46 52 10.65 7.43 8.5 9.6 74 81 32.8 38.2 49 50.8 1 1.1 7.55 14.54 148.3 147.1 5.81 5.19 107 110 BACK TO MAIN HIS-SUM 5 22-APR-99 1 Data are from Sprague Dawley control rats which received the vehicle (In general 0.9% NaCl) via continuous intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated. 117 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE PFHS/PFOS HISTORICAL DATA CLINICAL BIOCHEMISTRY1 ANIMALS OF 13-18 WEEKS OF AGE MALES AND FEMALES PARAMETER SEX N CALCIUM mmol/1 M F INORG. PHOSPH. M mmol/1 F 42 28 42 28 MEAN 2.53 2.5 2.6 2.1 ST. DEV. MIN. 0.14 0.17 0.24 0.23 2.23 2.26 2.17 1.65 MAX. 2.78 2.8 3.34 2.56 BACK TO MAIN HIS-SUM - 6 22-APR-99 1 Data are from Sprague Dawley control rats which received the vehicle (1n general 0.9% NaCl) via continuous Intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated. . 118 TELOMER ALCOHOL/TELOMER ACRYLATE/ VPFHS/PFOS BACK TO MAIN NOTOX 242933 TABLES - INDIVIDUAL DATA 119 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE -PFHS/PFOS ' MORTALITY DATA MALES GROUP 1 (VEHICLE CONTROL) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 1 03-N0V-98 2 03-N0V-98 3 03-N0V-98 4 03-NOV-98 5 04-N0V-98 6 04-N0V-98 7 04-N0V-98 8 04-N0V-98 9 18-N0V-98 10 18-N0V-98 11 18-N0V-98 13 02-DEC-98 14 02-DEC-98 15 02-DEC-98 06-0CT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-OCT-98 O6-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-0CT-98 02-N0V-98 0 2 -NOV-98 02-NV-98 02-N0V-98 0 3 -NOV-98 03-N0V-98 03-N0V-98 03-N0V-98 03-N0V-98 03-N0V-98 03-N0V-98 03-NOV-98 03-N0V-98 03-N0V-98 MALES GROUP 2 (TELOMER ALCOHOL) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 17 03-N0V-98 18 03-NOV-98 19 03-N0V-98 20 03-N0V-98 21 04-N0V-98 22 04-N0V-98 23 04-N0V-98 24 04-N0V-98 25 18-N0V-98 26 18-N0V-98 27 18-N0V-98 29 02-DEC-98 30 02-DEC-98 31 02-DEC-98 06-0CT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-0CT-98 02-N0V-9B 02-N0V-98 02-N0V-98 02-N0V-98 03-N0V-98 03-N0V-98 0 3 -NOV-98 03-N0V-98 0 3 -NOV-98 03-N0V-98 O3-N0V-98 03-N0V-98 03-N0V-98 03 -NOV-98 . BACK TO MAIN MORT-IND - 1 24-FEB-99 ' 1 20 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS " MORTALITY DATA MALES . GROUP 3 (TELOMER ACRYLATE) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 33 03-NOV-98 34 03-NOV-98 35 03-NOV-98 36 03-NOV-98 37 04-NOV-98 33 04-N0V-98 39 04-N0V-98 40 04-NOV-98 41 18-N0V-98 42 18-N0V-98 43 18-N0V-98 45 02-DEC-98 46 02-DEC-98 47 02-DEC-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 02-NOV-98 02-N0V-98 02-NOV-98 02-NOV-98 03-N0V-98 03-NOV-98 03-N0V-98 03-NOV-98 03-NOV-98 03-NOV-98 03-N0V-98 03-N0V-98 03-NOV-98 0 3 -NOV-98 . MALES GROUP 4 SCHEDULED ANIMAL NECROPSY 49 03-N0V-98 50 03-NOV-98 51 03-N0V-98 52 03-NOV-98 53 04-N0V-98 54 04-N0V-98 55 04-N0V-98 56 04-N0V-98 57 18-N0V-98 58 18-NOV-98 59 18-N0V-98 61 02-DEC-98 62 02-DEC-98 63 02-DEC-98 OTHER TREATMENT FROM TO 06-OCT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 02-NOV-98 02-NOV-98 02-N0V-98 02-N0V-98 03-NOV-98 03-NOV-98 03-N0V-98 03-NOV-98 O3-N0V-98 03-NOV-98 0 3 -NOV-98 03-NOV-98 03-NOV-98 03-N0V-98 BACK TO MAIN MORT-IND - 2 2 4 -FEB-99 . . 121 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -_ PFHS/PFOS MORTALITY DATA MALES GROUP 5 (PFHS) SCHEDULED ANIMAL NECROPSY OTHER 65 03-NOV-98 66 03-NOV-98 67 03-N0V-98 68 03-NOV-98 69 04-NOV-98 70 04-NOV-98 71 04-N0V-98 72 04-NOV-98 73 18-NOV-98 74 18-N0V-98 75 18-NOV-98 77 02-DEC-98 78 02-DEC-98 79 02-DEC-98 TREATMENT FROM TO 06-0CT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-OCT-98 02-NOV-98 02-NOV-98 02-NOV-98 02-NOV-98 03-NOV-98 03-NOV-98 . 03-N0V-98 03-NOV-98 03-NOV-98 03-NOV-98 03-N0V-98 03-NOV-98 03-NOV-98 03-NOV-98 MALES GROUP 6 (PFOS) SCHEDULED ANIMAL NECROPSY 81 03-N0V-98 82 03-NOV-98 83 03-N0V-98 84 03-N0V-98 85 04-N0V-98 86 04-NOV-98 87 04-NOV-98 88 04-N0V-98 89 18-NOV-98. 90 18-NOV-98 91 18-N0V-98 93 02-DEC-98 94 02 -DEC-98 95 02-DEC-98 OTHER TREATMENT FROM TO 06-0CT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-OCT-98 02-NOV-98 02-NOV-98 02-N0V-98 02-N0V-98 03-NOV-98 03-NOV-98 03-N0V-98 03-N0V-98 03-NOV-98 03-NOV-98 03-NOV-98 03-NOV-98 03-N0V-98 03-NOV-98 BACK TO MAIN MORT-IND 3 2 4 -FEB-99 . ' 1 22 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ PFHS/PFOS MORTALITY DATA FEMALES GROUP 1 (VEHICLE CONTROL) . SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 97 05-NOV-98 98 05-NOV-98 99 05-NOV-98 100 05-N0V-98 101 06-NOV-98 102 06-NOV-98 103 06-NOV-98 104 06-NOV-98 105 20-NOV-98 106 20-NOV-98 107 20-NOV-9B 109 04-DEC-98 110 04-DEC-98 111 04-DEC-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98 04-N0V-98 04-NOV-98 04-NOV-98 0 4 -NOV-98 05-N0V-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 0 5 -NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 0 5 -NOV-98 FEMALES GROUP 2 (TELOMER ALCOHOL) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 113 05-NOV-98 114 05-NOV-98 115 05-N0V-98 116 05-NOV-98 117 06-NOV-98 118 06-NOV-98 119 06-NOV-98 120 06-NOV-98 121 20-NOV-98 122 20-N0V-98 123 20-N0V-98 125 04-DEC-98 126 04-DEC-98 127 04-DEC-98 08-OCT-98 08-0CT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98 04-NOV-98 0 4 -NOV-98 04-NOV-98 04-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-N0V-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 BACK TO MAIN MORT-IND - 4 24-FEB-99 1 23 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -,, /PFHS/PFOS MORTALITY DATA FEMALES GROUP 3 (TELOMER ACRYLATE) SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 129 130 05-NOV-98 131 05-NOV-98 132 05-NOV-98 133 06-NOV-98 134 06-NOV-98 135 06-NOV-98 136 06-NOV-98 137 20-NOV-98 138 20-N0V-98 139 20-N0V-98 141 04-DEC-98 142 04-DEC-98 143 04-DEC-98 05-N0V-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 04-N0V-98 04-NOV-98 0 4 -NOV-98 04-N0V-98 0 5 -NOV-98 05-NOV-98 0 5 -NOV-98 05-N0V-98 05-N0V-98 05-NOV-98 05-N0V-98 05-NOV-98 05-N0V-98 05 -NOV-98 FEMALES GROUP 4 SCHEDULED ANIMAL NECROPSY OTHER TREATMENT FROM TO 145 05-NOV-98 146 05-N0V-98 147 05-NOV-98 148 05-N0V-98 149 06-NOV-98 150 06-N0V-98 151 06-NOV-98 152 06-N0V-98 153 20-NOV-98 154 20-NOV-98 155 20-NOV-98 157 04-DEC-98 158 04-DEC-98 159 04-DEC-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98 04-NOV-98 04-NOV-98 04-NOV-98 04-N0V-98 05-NOV-98 0 5 -NOV-98 05-N0V-98 0 5 -NOV-98 05-N0V-98 0 5 -NOV-98 05-NOV-98 05-NOV-98 0 5 -NOV-98 05-NOV-98 BACK TO MAIN MORT-IND - 5 24-FEB-99 1 24 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -PFHS/PFOS ' MORTALITY DATA FEMALES GROUP 5 (PFHS) SCHEDULED ANIMAL NECROPSY OTHER 161 05-NOV-98 162 05-NOV-98 163 05-NOV-98 164 05-NOV-98 165 06-N0V-98 166 06-NOV-98 167 06-NOV-98 168 06-NOV-98 169 20-N0V-98 170 20-NOV-98 171 20-NOV-98 173 04-DEC-98 174 04-DEC-98 175 04-DEC-98 FEMALES GROUP 6 (PFOS) SCHEDULED ANIMAL NECROPSY OTHER 177 05-N0V-98 178 05-NOV-98 179 05-N0V-98 180 05-NOV-98 181 06-NOV-98 182 06-NOV-98 183 06-NOV-98 184 06-NOV-98 185 20-NOV-98 186 20-N0V-98 187 20-NOV-98 189 04-DEC-98 190 04-DEC-98 191 04-DEC-98 TREATMENT FROM 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 0 8 -OCT-98 08-0CT-98 04-N0V-98 04-NOV-98 04-NOV-98 04-N0V-98 05-NOV-98 05-N0V-98 05-NOV-98 05-N0V-98 05-NOV-98 05-NOV-98 05-NOV-98 0 5 -NOV-98 05-NOV-98 05-NOV-98 TREATMENT FROM TO 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 04-N0V-98 04-NOV-98 04-N0V-98 04-NOV-98 05-NOV-98 0 5 -NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 0 5 -NOV-98 05-NOV-98 - BACK TO MAIN MORT-IND - 6 24-FEB-99 ' 1 25 BACK TO MAIN NOTOX Project 242933 SYM-IND - 1 25-FEB-99 CLINICAL SIGNS MALES GROUP 1 (VEHICLE CONTROL) .. SIGN (MAX.GRADE) TREATMENT ' WEEKS: 1 ............ 2 .............3 ............ 4 ............ 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB ANIMAL 2 SKIN / FOR / PLUMAGE ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) ALOPECIA (3) (FORELEG LEFT) ALOPECIA (3) (HINDLEG LEFT) ALOPECIA (3) (HINDLEG RIGHT) G: .............. : ........................... 111111111... G: .......................... 1111111111111111111 G: ...1222222222221111111111111111111111111...... G: ..... 11111111111113311111111111111............ G: .... 1111111111111111111111111111111111...... ANIMAL 3 SKIN / FOR / PLUMAGE ALOPECIA (3) (CHEST) SCABS (3) (NECK) G: .11. G: . .111111111111111111111111111. ANIMAL 4 . SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) G: G: .111111 1111111 ANIMAL 9 SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (CHEST) . G: G: 111111111111111111111111111111111111.. ANIMAL 15 VARIOUS BULGING EYE (1) (EYE RIGHT) DARK (3) ' (EYE RIGHT) G: G: NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP G: Highest grades Time,after treatment (H.Mi) A: 0.00 B: 4.00 1 26 NOTOX Project 242933 CLINICAL SIGNS, DAILY MALES GROUP 1 (VEHICLE CONTROL) SIGN (MAX.GRADE)' (LOCATION) RECOVERY WEEKS: 1.... 2.... 3.....4.... 5. ANIMAL 9 SKIN / FDR / PLUMAGE ALOPECIA (3) (CHEST) . G ...... 12222222. ANIMAL 15 . VARIOUS BULGING EVE (1) (EYE RIGHT) DARK (3) (EYE RIGHT) G: 11....... G: 11....... NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM-IND - 2. 25-MAR-99 G: Highest daily grades 1 27 BACK TO MAIN NOTOX Project 242933 SYM-IND - 3 25-FEB-99 CLINICAL SIGNS MALES GROUP 2 (TELOMER ALCOHOL) *. SIGN (MAX.GRADE) . TREATMENT i,,,.........2 ..... ...... 3............4.... ...... ,5. DAYS:' l!2!3!4!5.'6!7!l!2!3!4!5.6!7.l!2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: AHABABABABABABABABABABABABABABABABABABABABABABABABABABABAB ANIMAL 17 SK IN / FOR / PLMAGE ALOPECIA (3) (NECK) ANIMAL 1 8 ::::::::::::::::::::::::::: G : .............................: ........................................ ; ..............................1 1 1 1 1 .............. : : : : : ..................... : : : : : : : : : : : : : : : : SK IN / FOR / PLUMAGE SCABS (3) (BACK) ANIMAL 1 9 G : ......................................................................................................................1 ............ : : : : : : : : : : : : : : : : : ..................... : : : : SK IN / FUR / PLUMAGE ALOPECIA (3) (SMOULDER LEFT) ANIMAL 20 :::::::::::::::::::::::::: G : ...............................................................................................1 1 ................................ : : : : : : : : : : : : : : : : : : .......... : : SK IN / FUR / PLUMAGE WOUND (3 ) (GENITAL REGION) : : : : : : : : : : : : : : ...............................: : : : : G : ......................................................................................................................1 ............ ANIMAL 2 1 : : ....................... : : : : : : : : : : : : : : : : : : : S K IN / FUR / PLUMAGE SCABS (3) (NECK) :::::::::::::::::::::::::: G : ....................................................................................................1 1 .............. 1 1 1 1 . ANIMAL 23 : : : : : : : : : : ....................... : : : : : : : : : : : SK IN / FUR / PLUMAGE ALOPECIA (3) (FORELEG LEFT) :::::::::::::::::::::::::: G : ................................................................................................................ 1 1 1 1 1 1 1 . ANIMAL 25 SKIN / FUR / PLUMAGE RED STAINING (3) (PERIORBITAL REGION LEFT) ANIMAL 26 G: SKIN / FUR / PLUMAGE SCABS (3) (SNOUT) ANIMAL 27 SKIN / FUR / PLUMAGE RED STAINING (3) (PERIORBITAL REGION RIGHT) G: G: 1 ANIMAL 29 ::::::::::::::::::::::::::::: BREATHING RALES (3) SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) ::::::::::::::::::::::::::::: G: ............................................... Ill G: .......... 1111111111111111111111111111111111111111111111 G: Highest grades T i m e ,after treatment (H.Mi) A: 0.00 B: 4.00 1 28 BACK TO MAIN NOTOX Project 242933 SYM-IND 25-FEB-99 CLINICAL SIGNS MALES GROUP 2 (TELQMER ALCOHOL) . SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 ............ 3 .............4 .............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB ANIMAL 30 SKIN / FUR / PLUMAGE ' ALOPECIA (3) (CHEEK RIGHT) G: NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP 111111111 G: Highest grades Tine after treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Project 242933 CLINICAL SIGNS, DAILY . MALES GROUP 2 (TELOMER ALCOHOL) SIGN (MAX.GRADE) (LOCATION) RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. ANIMAL 25 SKIN / FUR / PLUMAGE RED STAINING (3) (PERIORBITAL REGION LEFT) G: ...... Ill.... ANIMAL 26 SKIN / FUR / PLUMAGE SCABS (3) (SNOUT) G: 11............ ANIMAL 27 SKIN / FUR / PLUMAGE RED STAINING (3) (PERIORBITAL REGION RIGHT) G: ... 1 ....... ANIMAL 29 BREATHING RALES (3) SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) G: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 , G: 1 1 1 1 1 .......... . NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM-IND - S 25-MAR-99 G: Highest daily grades 1 30 BACK TO MAIN NOTOX Project 242933 SYM-IND - 6 2 5 -FEB-99 CLINICAL SIGNS MALES GROUP 3 (TELOMER ACRYLATE) SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 .............3 ............ 4 .............5. nAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB ANIMAL 33 SKIN / FOR / PLUMAGE ALOPECIA (3) (NECK) ALOPECIA (3) (CHEST) ALOPECIA (3) (FORELEG RIGHT) G: iiiiii!.!.! G: 111222222211111111111111111111111111 * G: 11122222221111111111............... ANIMAL 35 SKIN / FOR / PLUMAGE ALOPECIA (3) (FLANK LEFT) ALOPECIA (3) (CHEST) ALOPECIA (3) (FORELEG RIGHT) SCABS (3) (TAIL) G: 11112221111111111111 G: ................... G : ..111111111111111111 G: ................... mm inumimi.. ANIMAL 45 : : : : : : : : : : : : : : : : : : .......... SKIN / FOR / PLUMAGE SCABS (3) (BACK) RED STAINING (3) (NECK) : : : : : : : : : : : : : : : : i I ::: i i i G: ................... .................. 11...1111 .......... ....................................... NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP G: Highest grades Time'after treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Project 242933 CLINICAL SIGNS, DAILY MALES GROUP 3 (TELOMER ACRYLATE) SIGN (Max.GRADE) (LOCATION) RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. ANIMAL 45 SKIN / FUR / PLUMAGE RED STAINING (3) (NECK) G: .................. 111111 NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM-IND - 7 25-MAR-9 9 G: Highest daily grades 1 32 BACK TO MAIN NOTOX Project 242933 . S Y M - I N D - ff 2 5 - F E B - 99 CLINICAL SIGNS MALES GROUP 4 SIGN (MAX.GRADE) . TREATMENT - WEEKS: 1 ............ 2 ............ 3............ 4 ............ 5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABA3 ANIMAL 51 SKIN / FOR / PLUMAGE ALOPECIA (3) (FORELEG RIGHT) G: ANIMAL 56 ::::::::::::: SKIN / FUR / PLUMAGE RED STAINING (3) (PERIORBITAL REGION LEFT) : : : : ........... . : : G: .....................11111 ANIMAL 61 SKIN / FUR / PLUMAGE ALOPECIA (3) (EYE RIGHT) VARIOUS EYE RIGHT, INJURED (1) DEHYDRATED (3) (EYE RIGHT) BULGING EYE (1) (EYE RIGHT) DARK (3) (EYE RIGHT) G: G: G: G: G: ANIMAL 62 VARIOUS DARK (3) (EYE RIGHT) ............................................... ANIMAL 63 : : : : : : : : ........ : : : : : : : : : SKIN / FUR / PLUMAGE SCABS (3) (NECK) : : : : : : : : i : : : : i i i ': * : : : i G: ................................ 111111111111 NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP 11.. G: Highest grades Time .after treatment (K.Mi) A: 0.00 B: 4.00 1 33 NOTOX Project 242933 CLINICAL SIGNS, DAILY . MALES ' GROUP 4 SIGN {MAX.GRADE) (LOCATION) RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. ANIMAL 61 SKIN / FOR / PLUMAGE ALOPECIA (3) (EYE RIGHT) VARIOUS - EYE RIGHT. INJURED (1) DEHYDRATED (3) (EYE RIGHT) BULGING EYE (1) (EYE RIGHT) DARK (3) (EYE RIGHT) G: ........... llllllllllllli.il. g : liiiiiiiiiiiiiiiimumm. G: .22222...................... G: 111111...................... G: 111111...................... ANIMAL 62 VARIOUS DARK (3) (EYE RIGHT) G: 11 NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM-IND - 9 25-MAR-99 G: Highest daily grades 1 34 BACK TO MAIN NOTOX Project 242933 SYM-IND -lo 25-FEB-99 CLINICAL SIGNS KALES GROUP 5 (PFHS) STOW (MAX.GRADE) treatm en t WEEKS: 1 ............ 2 ............ 3 ...... ......4 .............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB ANIMAL 65 SKIN / FDR / PLUMAGE RED STAINING (3) (EAR LEFT) G: 11111111111 ANIMAL 67 SKIN / FUR / PLUMAGE RED STAINING (3) (SNOOT) G: ANIMAL 72 SKIM / FDR / PLDMAGE WOUND (3) (BACK) G ANIMAL 74 POSTURE HUNCHED POSTURE (1) . G: ANIMAL 79 SKIN / FUR / PLUMAGE RED STAINING (3) (NECK) RED STAINING (3) (CERVICAL REGION) VARIOUS DARK (3) (EYE RIGHT) G: G: G: NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP 111 1111 G: Highest grades Time After treatment (H.Mi) A: 0.00 B: 4.00 1 35 NOTOX Proiact 242933 CLINICAL SIGNS, DAILY MALES GROUP 5 (PFHS) S IG N (MAX.GRADE) (LOCATION) RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. ANIMAL 74 POSTURE HUNCHED POSTURE (1) G: 11... ANIMAL 79 SKIN / FUR / PLUMAGE RED STAINING (3) (NECK) RED STAINING (3) (CERVICAL REGION) VARIOUS DARK (3) (EYE RIGHT) G: .... , . . 1 1 .................. G: .... ....1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 . G: 11... NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM -IN D - 1 1 25-MAR-99 G: Highest daily grades 1 36 BACK TO MAIN NOTOX Project 242933 SYM-IND -12 25-FEB-99 CLINICAL SIGNS HALES ' GROUP 6 (PFOS) SIGN (MAX.GRADE) . TREATMENT WEEKS: 1 ............ 2 ............ 3 ............ 4 .............5. DAYS: 1.2.3.4,5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME; ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB ANIMAL 81 SKIN / FUR / PLUMAGE SWELLING (3) (ABDOMEN) . G: ANIMAL 87 SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEEK LEFT) :::::::::::::::::::: G: .......................... 11111111111111 ANIMAL 93 SKIN / FUR / PLUMAGE SCABS (3) (NECK) !:i! G: ................................. 1111111 ANIMAL 95 :::::: SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) :::::: G: ........... NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP mm.. G: Highest grades Time .after treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Project 242933 CLINICAL SIGNS, DAILY MALES GROUP 6 (PFOS) SIGN (MRX.GRADE) (LOCATION) RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. ANIMAL 95 SKIN / FOR / PLUMAGE RED STAINING (3) (HEAD) G : .............................................. 1 1 1 1 1 1 1 1 1 NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM-IND -13 2 5 - M A R - 99 G: Highest daily grades 1 38 BACK TO MAIN NOTOX Project 242933 SYM-IND - M25-FEB-99 CLINICAL SIGNS FEMALES GROUP 1 (VEHICLE CONTROL) . S IG N (MAX.GRADE) TREATMENT wp'Rtf.Q 1 .# 2................. 3 ......................... 4 . . 5 D A Y S :' l ! 2 ! 3 . ' 4 ! 5 .' 6.' 7 ! l ! 2 ! 3 ! 4 ! 5 ! 6 ! 7 ! l ! 2 . ' 3 ! 4 ! 5 ! 6 ! 7 ! l ! 2 ! 3 ! 4 . ' 5 !6 .' 7 ! l . ' TIM E: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB ANIMAL 97 VARIOUS MISSING (1) (TAIL APEX) ANIMAL 98 VARIOUS MISSING (1) (TAIL APEX) ANIMAL 104 SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK) ANIMAL 105 SKIN / FUR I PLUMAGE SCABS (3) (ABDOMEN) WOUND (3) (ABDOMEN) ANIMAL 106 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) ANIMAL 109 SKIN / FUR / PLUMAGE ALOPECIA (3) (FLANK RIGHT) ALOPECIA (3) (THIGH HIND LEFT) ALOPECIA (3) (THIGH HIND. RIGHT) ANIMAL 110 SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) SECRETION / EXCRETION SALIVATION (3) ANIMAL 111 SKIN / FUR / PLUMAGE ALOPECIA (3) (GENERAL) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (BACK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (FLANK LEFT) ALOPECIA (3) (FLANK RIGHT) G: 1 U U 1 U 1 1 I U U 1 1 1 1 1 U I U 1 1 U U .. G: u u i i m n u u i i i i i u u u i u u .. :::::::::::::::::::::::: : : : .............. G: ...............................111111111...... : : : : : : : : : : : : : : ............ : : : : : : : : G: ................................................... 1111111 G: .................................................. 1 ...... .............. G: : ********* G: G: . . . 1 1 1 1 1 1 1 1 1 1 1 ................. . G: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ................. .1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 .... Ill G: ........... 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 G: * G: ************** G: G: G: 1 1 1 1 2 2 2 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ...... G: G: * ** ******** 1111111111111111111 G: Highest grades Time^after treatment (H.Mi) A: 0.00 B: 4.00 1 39 BACK TO MAIN NOTOX Project 242933 SYM -IN D - IS 25-F E B -9 9 CLINICAL SIGNS FEMALES . GROUP 1 (VEHICLE CONTROL) . SIG N (MAX.GRADE) ALOPECIA (3) (SHOULDER LEFT) ALOPECIA (3) (HINDLEG LEFT) TREATMENT WEEKS: 1 ............................... 2 ................................3 ............................... 4 ........................... 5 DAYS: 1 . 2 . 3 . 4 . 5 . 6 . 7 . 1 . 2 . 3 . 4 . 5 . 6 . 7 . 1 . 2 . 3 . 4 . 5 . 6 . 7 . 1 . 2 . 3 . 4 . 5 . 6 . 7 . 1 . TIM E :' ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB G: ........................................................................1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ................. G : ......................................................................................... 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 .............. .. NO FURTHER C L IN IC A L SIGN S NOTED IN T H IS GROUP G: Highest grades Time after treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Project 242933 CLINICAL SIGNS, DAILY FEMALES GROUP 1 (VEHICLE CONTROL) SIGN (MAX.GRADE) (LOCATION) RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. ANIMAL 105 SKIN / FUR / PLUMAGE SCABS (3) (ABDOMEN) . G: ...1111111111.. ANIMAL 106 SKIN / FUR /. PLUMAGE ALOPECIA (3) (NECK) G: ......... 1111. ANIMAL 109 SKIN / FUR / PLUMAGE ALOPECIA (3) (FLANK RIGHT) ' ALOPECIA (3) (THIGH HIND LEFT) ALOPECIA (3) (THIGH HIND RIGHT) G: ....... 1111... G: ...111111111111 G: 111111111111111 ANIMAL 111 SKIN / FUR / PLUMAGE ALOPECIA (3) (GENERAL) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (BACK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (FLANK LEFT) ALOPECIA (3) (FLANK RIGHT) G: ................ 11111111111 G ............ 11.......... G: ..... 11111111111.......... G ........11111111111.......... G: 11111111111111111 G: 11122222222222222 NO FURTHER CLINICAL SIC2JS NOTED IN THIS GROUP BACK TO MAIN SYM-IND -l6 25-MAR-99 G: Highest daily grades BACK TO MAIN NOTOX Project 242933 SYM-IND - 17 25-FEB-99 CLINICAL SIGNS FEMALES . GROUP 2 (TELOMER ALCOHOL) ., SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 .............3 ............ 4 .......... ..5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABARABABABABABABABABABARABABABABABABABABABABABABABABABABAB ANIMAD 113 SKIN / FUR / PLUMAGE ALOPECIA (3) (FORELEGS) G: ANIMAL 117 SKIN / FUR / PLUMAGE RED STAINING (3) (EAR RIGHT) G: .11. ANIMAL 119 SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK) G: ANIMAL 121 SKIN / FUR / PLUMAGE ALOPECIA (3) (HEAD) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (CHEEK RIGHT) ALOPECIA (3) (NECK) SCABS (3) (FLANK LEFT) G: G: G: G: G: ANIMAL 122 SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (CHEEK RIGHT) SCALES (3) (CHEEK LEFT) SCALES (3) (CHEEK RIGHT) G: G: G: G: ANIMAL 123 SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEEK LEFT) G: ANIMAL 126 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) G: 11111111111111111. NO FURTH ER CLINICAL SIGNS NOTED IN THIS GROUP .1111111112.. .11111 .11 . 11. .1111111111. G: Highest grades Time,after treatment (H.Mi) A: 0.00 B: 4.00 1 42 NOTOX Project 242933 ~" CLINICAL SIGNS, DAILY FEMALES GROUP 2 (TELOMER ALCOHOL) SIGN (MAX.GRADE) (LOCATION) RECOVERY WEEKS: ANIMAL 121 . SKIN / FUR / PLUMAGE ALOPECIA (3) (HEAD) ALOPECIA (3) (CHEEK LEFT) ALOPECIA' (3) (CHEEK RIGHT) G: ...... 2222211 G .1112222222222. G ___ 1222222222. ANIMAL 122 SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (CHEEK RIGHT) SCALES (3) (CHEEK LEFT) SCALES (3) (CHEEK RIGHT) G .... 112222222. G ___ 1222222222. G ...... 1 1 1 1 1 1 1 . G ...... 1 1 1 1 1 1 1 . NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM-IND -IS 2 5 - M A R -9 9 G: Highest daily grades 1 43 BACK TO MAIN NOTOX Project 242933 SYM-IND - 1g 25-FEB-99 CLINICAL SIGNS FEMALES GROUP 3 (TELQMER ACRYLATE) . SIGN (MAX.GRADE) treatment WEEKS: 1 .......... ..2.............3 ............ 4 ............ 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIM E: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB ANIMAL 129 POSTURE HUNCHED POSTURE (1) ANIMAL 131 SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEST) RED STAINING (3) (HEAD) RED STAINING (3) (NECK) ANIMAL 132 SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (SHOULDER LEFT) RED STAINING (3) (HEAD) RED STAINING (3) (BACK) RED STAINING (3) (NECK) ANIMAL 133 POSTURE HUNCHED POSTURE (1) ANIMAL 134 SKIN / FUR / PLUMAGE SCABS (3) (NECK) ANIMAL 136 . SKIN / FUR / PLUMAGE SCABS (3) (BACK) RED STAINING (3) (HEAD) ANIMAL 137 SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) ANIMAL 138 POSTURE . HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE PILOERECTION (1) ALOPECIA (3) (ABDOMEN) :::::::::::::::::: G: ............ ;............. 111111111 ...... G: ___ G: ___ :: G: ___ G: ___ G: .... G: ___ G: .... * * 1 * * .......... ..1 1 1 1 1 1 1 ......... ....... m i .... ... ....... ........................................................ .... G: ........... .ililililili.!. ! ::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::: G: .................................................... 111111 G: G: ::::::::::: G: 1111222211111.111.... .11 1 G: G: ............................................. G: ...................................... 1111111 iiiiiiii G: Highest grades Tine After treatment (H.Mi) A: 0.00 B: 4.00 1 44 BACK TO MAIN NOTOX Project 242933 S Y M-IND - 20 25-FEB-99 CLINICAL SIGNS FEMALES GROUP 3 (TELOMER ACRYLATE) .. SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 ............ 3 ............ 4 ............ 5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 . 7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1. TIME: ABABABABABABABABAHABABABABABABABABABAEABABABABABABABABABAB ANIMAL 139 . POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) G: G: ............................................. 1 1111111111111122222122222222222222222211111111 ANIMAL 141 :::::::::::::::::: SKIN 1 FDR / PLUMAGE RED STAINING (3) (HEAD) : .................. : : : : : : : : G: ................................... ANIMAL 142 : : : : : : : : : ................ SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) G: .................... .....111...... ANIMAL 143 : : : : : : .......... : : : : : : SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDCMEN) FISSURES WITH BLEEDINGS (3) (PAW HIND RIGHT) RED STAINING (3) (PAW HIND RIGHT) :::::::::::::::::::::::::::: : G: 1111222211111111.1111111.111111111111111111111111111111111 G: .......................................11................. G: .........................................111111111111111.. NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP G: Highest grades Time.after treatment (H.Mi) A: 0.00 B: 4.00 1 45 NOTOX Project 242933 CLINICAL SIGNS, DAILY FEMALES GROUP 3 (TELOMER ACRYLATE) SIGN (MAX.GRADE) (LOCATION) RECOVERY WEEKS: 1...;..2..... 3 ..... 4 ..... 5. ANIMAL 138 POSTURE h u n c h e d POSTURE (1) SKIN / FUR / PLUMAGE PILOERECTIQN (1) G: 11111111111111. G: .1111...... ANIMAL 139 POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) G: 1111111....... G: 11111111111111. ANIMAL 141 SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) G: ................ ......... ANIMAL 142 SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) G: .......................... NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM-IND -21 2 5 - M A R - 99 G: Highest daily grades 1 46 BACK TO MAIN NOTOX Project 242933 S Y M - I N D - 22. 25-FEB-99 CLINICAL SIGNS FEMALES GROUP 4 ( ) SIGN (MAX.GRADE) TREATMENT . WEEKS: 1 ............ 2 .............3 ............ 4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1. TIME: a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a h a b a b a b a b a b a b a b a b a b a b a b ANIMAL 147 SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK) G: 111111111111. . .1111111111111____ 1111111 ANIMAL 148 SKIN / FUR / PLUMAGE SHELLING (3) (EAR RIGHT) RED STAINING (3) (EAR LEFT) RED STAINING (3) (EAR RIGHT) G: G: G: .1111111111111... .......... 111111111......... ......... .11111111111111111111111111111111111111 ANIMAL 151 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) G: .11111111 ANIMAL 152 POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE RED STAINING (3) (EAR RIGHT) G; G: . . .1111111111111111111111111111 111111111111111111111111111111111111 ANIMAL 155 . SKIN / FUR / PLUMAGE RED STAINING (3) (EAR RIGHT) G: ................................................ 1111111111 ANIMAL 157 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) ALOPECIA (3) (THROAT REGION) SCALES (3) (THROAT REGION) ' G: G: G: .222222222222222222222222222222221111 . 1111111111111. ANIMAL 159 SKIN / FUR / PLUMAGE ALOPECIA (3) G: (NECK) SCABS (3) G: (NECK) RED STAINING (3) G: (EYE RIGHT) RED STAINING (3) G: (PERIORBITAL REGION RIGHT) SECRETION / EXCRETION WATERY DISCHARGE FROM EY (3) G: (EYE RIGHT) .111111111111111111111111111111111111 NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP G: Highest grades Time -after treatment (H.Mi) A: 0.00 B: 4.00 1 47 N O T O X Pro-iect 242933 CLINICAL SIGNS, DAILY FEMALES GROUP 4 ( ) SIGN (MAX.GRADE) (LOCATION) RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5. ANIMAL 159 SKIN / FUR / PLUMAGE SCABS (3) G: ....... (NECK) RED STAINING (3) G: ...11... (EYE RIGHT) RED STAINING (3) G: .... 11. (PERIORBITAL REGION RIGHT) SECRETION / EXCRETION WATERY DISCHARGE FROM EY (3) ............ . (EYE RIGHT) .11 NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN S Y M - I N D - 2.3 2S-MAR-99 G: Highest daily grades 1 48 BACK TO MAIN NOTOX Project 242933 SYM-IND - 54 25-FEB-99 CLINICAL SIGNS FEMALES GROUP 5 (PFHS) SIGN (MAX.GRADE) TREATMENT WEEKS: 1 ............ 2 ............ 3 .............4 ............ 5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1. TIME: a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b ANIMAL 162 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) SCABS (3) (NECK) WOUND (3) (NECK) G: G: 1111112222111111111111222222111111111111111111111111111... G: ANIMAL 163 POSTURE HUNCHED POSTURE (1) G: ANIMAL 166 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) G: ..................... 1111111111. ANIMAL 167 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) " G: .111111111111111. ANIMAL 169 SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK) SECRETION / EXCRETION SALIVATION (3) G: G: ANIMAL 173 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) ' (SHOULDER RIGHT) G: G: G: .11111111111111111111132. ...... i m m u n i . .. ANIMAL 174 SKIN / FUR / PLUMAGE - ALOPECIA (3) (BACK) ALOPECIA (3) (ABDOMEN) G: G: ....... 11111112111111. .1111111111222222222222222222222222111111111111 ANIMAL 175 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) ALOPECIA (3) (HINDLEG RIGHT) G: G: .illuminili. NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP G: Highest grades Time'after treatment (H.Mi) A: 0.00 B: 4.00 1 49 NOTOX Project 242933 CLINICAL SIGNS, DAILY FEMALES GROUP 5 (PFHS) S IG N (MAX.GRADE) (LOCATION) RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5 . ANIMAL 173 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) G: .... 11111111111111111111111 ANIMAL 174 SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK) ALOPECIA (3) (ABDOMEN) . G: ........................ u G: 111----111111111111111111111 ANIMAL 175 SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) ALOPECIA (3) (HINDLEG RIGHT) G: 111...... 111111111111111111 G: ..... 1111........ ........... NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM-IND - 25 2 5 - M A R - 99 G: Highest daily grades BACK TO MAIN NOTOX Project 242933 -- SYM-IND 2 5 -- F E B * 99 CLINICAL SIGNS FEMALES GROUP 6 (PFOS) SIGN (MAX.GRADE) . TREATMENT WEEKS : 1 ............ 2 .............3 ............ 4 ............ 5. DAYS: 1 .2 . 3 . 4. 5 . 6.7 .1 . 2 . 3 .4 .5 . 6.7 . 1.2 .3 . 4. 5. 6. 7 . 1.2. 3 . 4 . 5. 6. 7 .1 . TIME! ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB ANIMAL 178 POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE RED STAINING (3) (SNOUT) G G 111111............. ............... 1111... ANIMAL 179 POSTURE HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE ALOPECIA (3) (FORELEG LEFT) ALOPECIA (3) (FORELEG RIGHT) G G G ..... 11111111.............. 111111112. . 222222222222222.. 222222222. . 222222222222222. . ANIMAL 183 ' SKIN / FUR / PLUMAGE SCABS (3) (NECK) G: 1111111 ANIMAL 185 SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) G: ANIMAL 186 SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) RED STAINING (3) (HEAD) G: G: liiiiiiiiiiiiiiiiiiiiiii.!. ! ANIMAL 187 SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) RED STAINING (3) (EAR LEFT) RED STAINING (3) (EAR RIGHT) FED STAINING (3) (NECK) ANIMAL 189 SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) . . . . . . . . .............................. G: ............... 1111...................................... ANIMAL 190 ........... .. : : ............. : ...........: : : : : : SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) :::::::::: i : ! ! ! !i !i ::::::: i : : G: .........................111111..... 111111... 111111111111 NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP G: Highest grades Time After treatment (H.Mi) A: 0.00 B: 4.00 NOTOX Project 242933 CLINICAL SIGNS, DAILY FEMALES GROUP 6 (PFOS) SIGN (MAX.GRACE) (LOCATION) RECOVERY ' WEEKS: 1 ..... 2 ..... 3,.... 4 ..... 5. ANIMAL 185 SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) G: . . . 1 1 ......... ANIMAL 186 SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) G: ...1 1 1 1 1 1 1 1 1 1 1 . ANIMAL 187 SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) RED STAINING (3) (EAR LEFT) RED STAINING (3) (EAR RIGHT) RED STAINING (3) (NECK) G: 1113211.... 1 1 . G: ...1 1 1 1 1 1 1 1 1 ... G; ........ 1 1 1 ... G: ANIMAL 190 SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) G: 1 1 1 1 1 .......... NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP BACK TO MAIN SYM-IND - 27 2 5 -MAR-99 G: Highest daily grades 1 52 BACK TO MAIN NOTOX Project 242933 BODY WEIGHTS (GRAM) MALES ~~ BW-IND - 1 1 7 -M A R -9 9 TREATMENT DAYS MEEKS ANIMAL 1 1 5 1 GROUP 1 (VEHICLE CONTROL) 1 232 219 2 202 253 3 228 252 4 196 214 5 209 235 6 221 249 7 222 245 8 197 221 9 207 236 10 184 206 11 207 232 12 -- -- -- 13 208 238 14 190 217 15 211 233 16 GROUP 2 (TELCMER ALCOHOL) 17 224 245 18 230 261 19 219 241 20 209 235 21 189 217 22 228 254 23 216 241 24 228 248 25 205 230 26 190 209 27 197 223 28 ------ ...... 29 208 232 30 217 249 31 212 234 32 GROUP 3 (TELQMER ACRYLATE) 33 220 243 34 191 216 35 224 242 36 211 233 37 220 243 38 211 236 39 206 231 40 206 232 41 216 245 42 197 220 43 210 235 44 -- -- --- 45 201 227 46 213 242 47 206 229 48 -- -- GROUP 4 49 222 239 50 201 228 51 217 246 52 211 244 S3 209 236 8 12 15 2 23 235 257 274 297 274 ' "300 230 246 258 278 270 288 269 297 244 266 247 274 207 229 240 264 ---- 250 2S1 227 254 243 270 262 311 313 255 288 304 316 280 277 226 277 . -- 285 262 275 265 286 299 280 307 329 259 277 288 251 270 285 238 270 287 277 304 321 262 288 302 264 284 300 237 265 265 217 237 245 235 256 260 -- -- ---- ---- 240 270 277 257 290 297 245 267 273 261 278 296 234 259 273 250 267 277 250 269 281 263 278 290 261 282 298 251 280 287 255 276 290 261 292 294 227 257 262 246 275 288 ---- 235 269 271 249 278 283 234 267 270 -" -- -- 248 268 278 244 268 280 264 294 310 267 294 308 252 278 288 1 53 19 22 25 3 44 281 289 301 332 341 354 341 351 360 271 282 288 301 308 322 319 334 350 341 349 368 297 304 317 303 310 322 245 251 260 295 310 316 ---- -- 311 323 345 286 299 309 309 319 328 319 329 354 359 311 322 300 307 .310 ' 322 343 348 322 328 307 321 289 292 269 277 283 288 -- '. 292 298 320 333 289 297 342 384 327 320 338 363 336 333 308 287 3_03 310 347 313 317 327 346 288 301 312 294 306 314 301 313 323 304 316 327 320 327 340 314 322 334 306 319 327 319 331 355 289 300 310 302 313 326 _ -- w ---- 304 310 326 304 311 325 298 313 324 ------ 294 303 308 301 303 316 336 345 . 360 335 338 347 315 324 346 28 4 309 365 377 298 330 356 381 322 336 276 328 -- 356 322 342 359 389 342 328 348 386 343 346 319 294 311 ---- 317 360 320 352 318 329 328 331 355 349 332 365 325 337 ---- 341 336 338 -- 324 333 378 360 354 BACK TO MAIN NOTOX Project 242933 BODY WEIGHTS (GRAM) MALES BW-IND - 2 17-MAR-99 RECOVERY DAYS WEEKS ANIMAL 1 1 .. 5 1 8 12 14 19 22 25 28 2223444 GROUP 1 (VEHICLE CONTROL) 1 2 ---- 3 4 5 6 7 8 9 318 348 10 253 279 11 310 339 12 13 334 370 14 297 337 15 314 354 16 -- -- GROUP 2 (TELOMER ALCOHOL) 17 18 -- 19 -- 20 21 22 23 24 25 298 330 26 277 303 27 289 318 28 29 289 315 30 336 385 31 296 334 32 -- -- -- ----- GROUP 3 (TELOMER ACRYLATE) 33 -- -- .34 -- -- -- 35 -- -- - -- 36 -- ---- ' 37 -- -- 38 -- -- 39 -- -- 40 ---- ---- 41 336 388 42 298 314 43 312 347 44 -- -- -- -- -- 45 314 352 46 312 351 47 310 351 48 SOUP 4 r 49 --. -- 50 -- -- 51 -- -- 52 -- -- 53 -- - -- -- 359 285 351 381 341 361 --- -- 337 308 330 317 388 345 -- ---- -- -- -- -- -- -- ---- 390 325 . 350 361 357 354 -- --- -- -- --- -- 364 299 362 394 348 379 -- -- 346 319 342 334 402 357 -- -- --- -- ---- -- -- --- -- 404 341 359 --.. 379 375 373 -- -- -- -- -- -- 373 303 366 398 353 378 --- -- 354 320 345 328 401 358 -- -- -- -- --- -- -- ---- 408 347 359 ... . 378 379 372 ... -- -- -- -- -- -- -- -- 414 365 393 -- -- -- -- -- 342 413 371 -- --- -- ---- -- -- -- ... ... -- ... ... 395 391 381 ____ ... -- -- -- -- -- -- -- 421 373 403 . -------- -- -- --- -- 435 385 415 --- -- -- -- -- 440 387 419 -------- ------ -- -- -- 349 416 381 -- . -------- -- -- -- 353 430 389 -- . --------- -- 355 429 388 -- -- -- -- -- ---- --- ____ ... -- ... ... 403 402 393 -- -- -- -- -- -- ... -- ... -- --... 412 411 398 ---- -- -- -- --- -- -- ... ... -- -- ---- 414 413 409 ... --. -- -- ... ... ... -- -- -- -- ... -- -- ... BACK TO MAIN NOTOX Project 242933 BODY WEIGHTS (GRM) MLES BW-IND - 3 17-MAR-99 TREATMENT DAYS WEEKS ANIMAL 1 5 8 12 15 19 22 25 28 112233444 GROUP 4 1 54 55 56 57 58 59 60 ' 61 62 63 223 206 200 206 203 199 229 215 205 64 _____ GROUP 5 (PFHS) 65 234 66 199 67 211 68 214 69 204 70 226 71 208 72 211 73 222 74 . 189 75 197 76 .--77 206 78 184 79 200 80 GROUP 6 (PFOS) 81 198 82 203 83 200 84 213 85 222 86 213 87 227 88 211 89 222 90 212 91 204 92 -- -- 93 187 94 220 95 211 96 -- 252 270 296 232 246 263 223 232 249 240 252 ' 290 235 247 270 228 237 266 264 278 317 238 253 283 230 240 268 ---- -- 257 217 232 233 225 257 234 . 237 254 203 220 ---- 233 210 226 282 231 248 249 246 281 258 259 268 201 231 --- 240 211 238 310 254 270 273 269 305 282 280 297 224 264 -- 267 233 255 300 268 252 298 281 276 327 288 274 --- 284 267 281 283 273 318 296 291 307 219 266 -- 272 236 255 221 242 263 283 231 2 52 2 7 5 283 228 248 ' 269 281 240 260 281 295 254 277 314 328 237 252 275 279 256 281 310 327 236 252 279 292 253 270 304 . 317 240 254 284 289 2-- -3-4 242 ---- 276 ------ 282 204 207 229 . 227 247 250 277 276 237 246 275 275 ---- -- -- 321 330 290 293 267 266 336 348 309 321 299 304 359 369 316 321 291 300 _____ ___ __ 351 364 282 285 300 304 301 310 296 295 343 355 315 317 310 314 338 343 241 245 296 307 --. 299 306 263 ' 271 276 285 300 310 298 302 300 307 311 314 360 371 294 295 350 361 312 320 343 352 321 335 318 ______ 333 . 242 247 299 305 295 301 ---- 340 352 306 313 278 285 374 398 334 348 314 325 380 397 345 359 316 329 ___ 381 392 298 299 315 327 322 324 308 . 315 375 379 330 337 329 334 365 380 255 256 321 333 ___ 318 327 287 290 291 301 322 331 312 327 316 325 333 338 388 394 311 322 380 381 336 345 374 386 349 364 351 362 -- 261 265 320 325 312 321 -- --- 1 55 BACK TO MAIN NOTOX Project 242933 BODY WEIGHTS (GRAM) MALES BW-IND - 4 1 7 - M A R - 99 RECOVERY DAYS WEEKS ANIMAL 1 1 GROUP 4 ( 54 55 -- 56 -- 57 365 58 321 59 301 60 -- 61 362 62 326 63 302 64 -- GROUP 5 (PFHS) 65 66 -67 -- 68 -- 69 -- 70 -- 71 -- 72 73 352 74 234 75 307 76 ------ 77 305 78 267 79 279 80 GROUP 6 (PFOS) 81 82 83 84 85 86 87 88 89 90 ---- -- -- -- -- ... 354 340 91 339 92 -- 93 245 94 306 95 293 96 5 8 12 14 19 22 25 28 12 22344 4 -- -- -- -- ---- -- -- -- -- -- ... -- ------ ... . ... 414 426 449 456 -- 363 367 383 383 -- 338 344 357 356 -- -- -- -- -- ---- ----- ... ... ... ... ... ... ---- -- ... ... ... 406 424 437 429 453 462 473 468 370 383 402 398 420 428 441 443 342 353 370 368 383 387 396 394 ------ -------- -- -- --- -- --- --- -- . -- --- ---- ---- -- -- -*-- -- ---- -- -- -- -- -- -- -- -- -- -- -- -- -- ... -- --- -- - -- -- -- .-- -- -- -- -- -- -- -- -- --. --- -- - . -- - -- .. ... ___ ... 397 412 420 418 -- ... ... __ 264 274 277 275 -- -- -- -- 342 350 365 361 ... ... ... ... --- ---- --- ------ -- ... ... ... 345 356 357 360 369 373 392 386 301 314 321 324 335 339 360 350 313 --- 319 325 --- 331 -- 337 -- 341 -- 353 -- 350 -- -- -- -- -- -- -- -- -- ---- -- -- -- . -- . .-- -- -- -- -- -- --- -- - -- -- -- -- -- -- -- .-- -- -- -- --- -- -- -- - -- . -- -- ---- --- -- -- -- -- .-- ---- ... __ -- .-- . -..-.- 408 412 428 429 __ _-- ... -- 380 384 397 404 -- -- -- ' -- 377 390 405 408 .-- ... -- . -- -- -- -- ------ -- -- ... ... ... ... 276 284 296 298 306 315 331 326 343 349 362 362 381 387 396 401 329 338 356 355 . 366 375 385 384 1 56 BACK TO MAIN N O T O X Project: 242933 BODY WEIGHTS (GRAM) FEMALES BW-IND - 5 1 7 -MAR--9 9 TREATMENT DAYS MEEKS ANIMAL 1 1 ' 5 1 GROUP 1 (VEHICLE CONTROL) 97 168 172 98 164 170 99 165 171 100 179 178 101 182 190 102 194 200 103 163 164 104 179 193 105 158 169 106 196 210 107 108 109 . 110 111 112 172 163 169 184 180 -- .162 180 192 GROUP 2 (TELCMER ALCOHOL) 113 170 176 114 182 191 115 182 184 116 180 188 117 157 167 118 168 174 119 163 168 120 166 182 121 163 170 122 163 171 123 175 183 124 ------ 125 162 165 126 164 168 127 166 182 128 GROUP 3 (TELCMER ACRYLATE) 129 170 179 130 154 161 131 171 171 132 175 180 133 153 164 134 172 181 135 181 192 136 166 170 137 157 164 138 167 177 139 158 157 140 -- -- 141 154 163 142 160 164 143 183 192 144 GROUP "4 ( ) 145 167 176 146 181 193 147 181 187 148 152 154 149 184 185 8 12 . 15 19 22 2 2 33 4 176 189 200 210 217 179 193 196 209 207 180 195 199 213 212 193 208 197 218 220 208 221 234 246 248 204 212 231 238 243 172 180 177 189 200 201 213 222 235 233 171 189 189 200 202 218 237 241 255 257 185 202 200 213 206 ~ - ---- ------ ... 166 179 181 188 195 185 204 200 219 215 197 211 216 230 235 184 206 195 194 173 176 178 191 168 169 187 ------ 172 166 184 191 220 203 207 184 190 193 198 178 184 200 --- 184 181 205 205 213 230 249 208 222 218 229 191 204 200 212 202 ' 211 211 218 178 189 180 193 204 216 ... ... 186 204 183 197 202 220 222 255 219 233 200 216 213 214 195 206 221 ... 203 199 223 185 192 190 205 204 167 177 175 186 191 186 195 201 211 212 193 201 209 212 221 166 176 180 191 192 192 197 198 206 218 198 216 222 237 244 183 192 184 199 204 167 181 188 204 212 176 184 182 . 185 183 162 175 176 191 197 -- -- ------ 169 186 1 S6 200 197 166 182 178 191 192 199 215 215 230 229 180 193 195 204 201 197 210 218 233 229 187 205 219 231 237 159 175 183 194 196 203 215 216 230 238 1 57 25 4 222 208 224 228 267 248 207 248 217 268 225 ... 199 238 243 233 266 234 242 214 227 220 238 208 212 236 ... 209 208 230 220 206 218 228 203 234 250 218 218 195 202 -- 203 205 249 217 248 242 207 240 28 4 220 217 227 244 268 251 206 252 219 275 232 ... 204 247 243 227 271 238 242 224 221 233 241 208 209 238 ... 216 211 236 225 208 222 231 203 236 249 215 214 201 199 -- 206 204 248 220 250 243 205 248 BACK TO MAIN NOTOX Project 242933 BODY WEIGHTS (GRAM) FEMALES ,, BW-IND - 6 1 7 - M A R - 99 RECOVERY DAYS WEEKS ANIMAL 1 1 5 1 8 12 14 222 GROUP 1 (VEHICLE CONTROL) 97 -- -- 98 99 100 101 ----- -- -- -- . -- 102 103 --- -- -- 104 -- 105 200 223 106 249 277 107 206 225 108 -109 185 2X0 110 217 245 1 1 1 222 251 112 -- --- GROUP 2 (TELCMER ALCOHOL) 113 114 115 116 117 118 119 120 121 ' 122 --------191 193 -- -- ---- -- *-- ---- 211 218 123 214 241 124 -- ------ 125 198 225 126 194 226 127 211 237 128 -- -- GROUP 3 (TELCMER ACRYLATE) 129 -130 -- -- 131 -- -- 132 -- -- 133 -- -- 134 -- ' -135 -- -- 136 -- -- -- 137 201 232 138 177 198 139 182 215 140 -- ------ 141 190 211 142 184 211 143 221 249 144 -- -- GROUP 4 ( ) 145 -146 -- -- 147 -- -- 148 -- -- 149 -- -- -- -- -- -- -- --- -- ---- 222 282 237 -- 214 257 254 -- -- -- -- -- -- -- ---- 227 288 243 ---- 219 264 262 ---- -- ---- -- -- -- -- .... 243 301 247 --- 220 267 261 --- -- --- -- ------ --- -- -- -- --- -- ------ -- --- -- ... .-- 215 223 217 222 232 223 244 254 255 ------ ---- -- 222 232 236 227 235 232 246 -- 2--52 2--58 --- -- - _ --- -- ---- ------ -- --- -- ------ -- ---- -- -- --- ... 235 247 244 199 212 211 215 229 224 ---- ------ -- 212 220 224 216 225 218 255 267 267 -- --- -- --- -- - -- -- ------ ------ -- --- -- 1 58 19 22 25 28. 344 4 -- -- - --. --- -- --- -- ---- -- -- -- -- 222 272 267 -- -- -- --. ---- ----... --. -- _ --- 226 275 274 -- -- -- -- -- -- -- -- -- -- -- . ' --- . 230 281 278 -- -- -- -- -- -- -- -- ... -- -- ... ... 237 290 278 -- -- -- -- -- - ------ -- --- -- -- ' --- --- -- -- -- .-- -- -- -- --- -- -- ... ... __ ___ -- ... -- -- ------ -- ... -- --- ... __ --~_ ... 243 247 248 244 234 240 242 244 260 -- 265 -- 2-6--3 266 ... -- ---- - -- ---- -- ------ ---- __ -... ___ ... ---- -- ... . _-- _ 232 239 228 229 276 277 ---- ----- -- -- : -- -- -- ... -- -- ... 235 233 278 -- -- .-- -- ... -- -- -- -- -- -- ... 232 234 278 -- -- --- -- ------ -- -- -- -- -- . -- -- -- --. BACK TO MAIN NOTOX Project 242933 BODY WEIGHTS (GRAM) FEMALES : BW-IND - 7 1 7 - M A R -9 9 TREATMENT DAYS WEEKS ANIMAL 1 1 5 1 8 12 15 19 22 25 28 2 233 44 4 GROUP 4 t ) 150 172 181 192 213 222 232 234 237 245 151 160 167 170 187 194 196 209 223 222 152 162 169 178 188 186 202 206 221 225 153 158 159 159 172 177 187 196 205 207 154 177 192 194 214 211 229 232 251 255 155 156 163 -- 169 -- 173 --- 191 -- 188 -- 199 -- . 2--06- 2_17 219 ___ 157 167 173 172 192 195 212 212 213 218 158 162 169 169 189 188 199 196 218 210 159 194 207 210 231 238 251 262 269 272 160 -- . -- -- -- -- -- -- -- -- GROUP 5 (PFHS) 161 166 171 179 191 192 205 208 210 216 162 169 177 185 203 200 211 210 222 227 163 167 172 179 187 182 197 204 214 214 164 171 172 181 191 194 207 206 218 223 165 157 155 167 180 191 202 210 214 220 166 173 172 187 195 203 216 226 237 234 167 166 162 164 180 189 200 202 209 2 11 168 185 190 197 213 224 233 240 250 250 169 177 189 191 204 203 223 221 231 236 170 167 174 177 187 186 200 203 217 215 171 168 183 187 204 199 214 222 232 237 172 -- -- ------ -- -- -- __ ___ 173 175 177 181 196 194 202 209 220 216 174 175 185 185 202 204 221 223 236 236 175 153 161 164 176 179 193 190 204 206 176 -- -- -- -- -- -- -- -- -- GROUP 6 (PFOS) 177 177 187 197 212 215 229 233 245 240 178 154 152 162 172 169 179 179 185 186 179 173 181 187 193 184 196 202 210 212 180 169 167 174 184 186 192 194 200 194 181 185 182 188 194 198 204 201 205 206 182 169 178 179 190 199 208 207 217 220 183 172 172 184 196 193 202 209 215 210 184 164 166 178 191 184 198 204 206 212 185 161 176 172 189 189 211 209 212 204 186 161 173 172 189 192 202 199 203 .206 187 188 157 -- 159 -- 168 --- 180 ------ 178 187 194 -- 201 -- 203. ---- 189 160 162 165 179 178 187 188 193 197 190 157 163 167 180 179 195 200 208 215 191 166 172 169 181 180 186 183 192 193 192 -- -- ---- -- -- ---- 1 59 B A C K TO MAIN NOTOX P r o j e c t 242933 BODY W EIGHTS (GRAM) FEM A LES : BW-IND - 8 17-MAR-99 recovery DAYS WEEKS ANIMAL 1 1 5 8 12 14. 19 22 25 28 12223444 GROUP 4 150 -151 -152 -153 189 154 230 155 201 156 -157 205 158 197 159 252 160 -- GROUP 5 (PFHS) 161 -162 -163 -164 -165 -166 -167 -168 -169 220 170 198 171 216 172 -173 200 174 216 175 188 176 -- GROUP 6 (PFOS) 177 --178 -179 -180 -181 -182 --183 -184 -185 188 186 187 187 184 188 -189 178 190 196 191 177 192 . -- ---- ---- ---- ---- 221 228 257 263 228 234 ---- -- 225 233 220 214 267 268 ---- ... -- -- -- ' -- -- -- -- 236 220 243 -- 226 249 211 -- -- -- -- -- -- -- -- 247 227 245 ---- 225 251 217 -- -- -- -- --. '--- -- ---- 216 212 207 197 216 194 " -- -- -- -- * -- -- 218 219 214 204 222 201 -- ------ -- --~ -- -- ----. 238 236 -- . 273 271 -- 238 234 --- ---- 237 235 232 222 237 240 280 277 286 ------ ... ___ ___ ---- -- -- --- -- ---- -- -- -- -- ------ --- -- --- -- -- ._ 248 248 __ 236 232 -- 258 257 ... ---- -- ... 233 226 238 256 254 258 227 -- 229 -- 234 -- -- -- -- --- -- - -- --- 226 221 223 ------ 209 227 209 -- -- --. --. -- -- -- 222 220 223 -- .. 215 228 213 ---- -- -- ------ -- __ __ -- ... ... 219 239 220 ... -- -- ... . . . ... -- ... ... '__ ... ... ___ ... ___ ... -- ----- -- ... ... ... 240 250 241 245 293 . 301 243 240 303 ---- -- ... ... __ -- --- ---- -- ... --- -- -- . . . . --- -- .-- -- ---- -- ... . . . ... ... ... ... ---- -- ... ... ... ... ... ... 239 250 249 263 273 269 237 -- 239 -- 244 -- ---- -- -- -- . .-- --. --. --- ---- -- -- ... ... ---- -- ... ... ... ... __ ... ---- -- __ ... ... __ __ ___ 218 219 221 235 238 244 218 220 222 ... -- ... 1 60 BACK TO MAIN NOTOX Project 242933 BODY WEIGHT GAIN (%) NALES BWG-IND - 1 17-MAR-99 TREATMENT DAYS WEEKS ANIMAL 1 1 5 1 GROUP 1 (VEHICLE CONTROL) 1 0 -6 2 0 25 3 0 11 4 09 5 0 12 6 0 13 7 0 10 8 0 12 9 0 14 10 0 12 11 12 ------0 --12 13 0 14 14 0 14 15 16 --0 --10 GROUP 2 (TELCMER ALCOHOL) 17 0 9 18 0 13 19 0 10 20 0 12 21 0 15 22 0 11 23 0 12 24 0 9 25 0 12 26 0 10 27 0 13 28 --- ------ 29 0 12 30 0 15 31 0 10 32 GROUP 3 (TELCMER ACRYLATE) 33 0 10 34 0 13 35 0 8 36 0 10 37 0 10 38 0 12 39 0 12 40 0 13 41 0 13 42 0 12 43 0 12 44 -- ... 45 0 13 46 O' 14 47 0 11 48 --- -- GROUP 4 49 . 0 50 0 51 ' 0 52 0 53 0 8 13 13 16 13 8 12 22 15 19 22 25 28 33 4 44 . 1 36 20 17 23 22 21 24 19 13 ----16* 20 19 --15-- 11 47 32 26 33 30 34 35 32 24 --28-- 35 34 - --2--8 13 54 37 30 38 38 42 42 34 23 ----34-- 37 38 --30 21 25 64 69 50 54 38 44 44 47 44 51 54 57 51 54 46 50 33 36 . .. . .5.0 50 55 51 57 --46- --51 30 75 58 47 54 58 66 61 56 41 _. .53. 66 63 55 33 81 65 52 58 61 72 63 62 50 . .5.8 71 69 --62 18 28 33 42 47 53 60 22 33 43 54 56 67 69 18 26 32 42 47 49 56 20 29 36 44 47 53 57 26 43 52 64 70 79 84 21 33 41 50 53 59 69 21 33 40 49 52 56 ' 59 16 25 32 35 41 46 . 52 16 29 29 41 42 50 56 14 25 29 42 46 51 55 19 30 32 44 46 54 58 -- ------ ... ... ... ... ... 15 30 33 40 43 49 52 18 34 37 47 53 60 66 16 26 29 36 40 48 51 19 26 35 23 36 43 12 19 24 18 27 33 20 26 32 24 34 41 22 36 39 24 34 41 21 35 36 15 30 33 17 31 37 --- ------ ... 17 34 35 17 31 33 14 30 31 -- ---- . ... 12 21 21 33 22 35 27 39 21 33 1 61 25 39 43 46 38 44. 51 31 43 38 52 52 49 48 47 __44 51 43 45 -- 49 58 37 48 44 55 56 55 , 53 52 49 ... 54 46 52 --. 57 63 40 53 49 61 62 59 64 57 55 --- 62 53 57 -- 32 36 39 50 51 57 55 59 66 59 60 64 51 55 66 60 66 47 55 50 68 69 61 69 65 60 ... 70 58 64 ... 46 66 74 71 69 BACK TO MAIN NOTOX Project 242933 B O D Y W E I G H T G A I N (%) MALES BWG- IND - 2 17 - M A R - 99 * RECOVERY DAYS 1 5 8 12 14 19 22 25 28 WEEKS 1 1 2 2 2 3 4 4 4- ANIMAL GROUP 1 (VEHICLE CONTROL) 1 -- -- -- 2 3 4 -- -- . -- --- -- -- ---- -- 5 6 --- -- - -- ------ 7 ------ 8 -- .... -- . 9 54 68 73 10 38 52 55 11 50 64 70 12 --- ---- 13 61 78 83 14 56 77 79 15 49 68 71 16 GROUP 2 (TELQMER ALCOHOL) - 17 18 19 20 21 22 23 24 25 26 -- ---- -- -- -- -- -- 45 46 -- -- - --- --- -- -- 61 59 27 47 61 28 ... ---- 29 39 51 30 55 77 31 40 58 32 GROUP 3 (TELCMER ACRYLATE) 33 -- -- 34 ... -- 35 -- -- . 36 -- 37 -- ... 38 ... , -39 -- --- 40 -- -- 41 56 80 42 51 59 -- ------ -- -- -- --* 64 62 68 ------ 52 79 63 -- -- --. ... .-- .-- . -- -- ai 65 43 49 65 67 44 .-- -- .-- 45 56 75 80 46 46 65 68 47 50 70 72 48 GROUP 4 ( 49 50 51 52 53 -- -- -- -- -- --- -- ---- ---- ... -- ---- -- ---- -- -- -- -- --. 76 63 75 ---- 89 83 80 -- -- -- -- ---- -- -- 69 68 74 ---- 61 85 68 -- ... ... -- -- -- -- ... 87 73 71 ... 89 76 81 -- -- .. -- -- -- ------ ---- -- -- -- ... -- -- -- --- ... ... ------ -- -- -- ... -- -- -- -- -- -- -- --- -- -- -- -- --- -- - ... ... ... 80 -- 65 -- --- -- __ ------ 77 -- -- ... ---- -- ... ... ... 91 99 102 109 112 86 92 96 103 104 79 86 91 97 99 ---- -- ... ... -- --- ---- -- -- ... -- ... ------ -- -- -- -- ,-- --- -- ------ -- -- ------ -- -- ... ... __ -- ... 73 .- ... -- 68 -- -- -- -- ' -- 75 -- ---- --- -- -- -- ... 58 64 68 70 71 85 90 92 98 98 69 75 80 83 83 -- --- --- . ---- --- -- ---- -- .-- -- ... ... 89 ... 76 -- 71 ... ... .-- 88 97 78 84 81 . 85 --. --- ... --- -- -- -- __ ... -- ... ... 100 89 91 -- --- ... -- -- . -- -- .-- ... -- -- ... 105 93 93 -- --. -- -- -- . ... ... .-- -- -- -- -- 106 94 99 ... ... __ ... -- ------ -- -- ------ -- -- -- -- -- -- ... ------ -- -- 1 62 BACK TO MAIN NOTOX Project 242933 BODY WEIGHT GAIN (%) MALES ; BWG-IND - 3 17-MAR-99 TREATMENT DAYS WEEKS ANIMAL 1 5 8 12 15 19 22 25 28 112233444 GROUP 4 ( ) 54 0 55 0 56 0 57 0 58 0 59 0 60 61 0 62 0 63 0 64 -- GROUP 5 (PFHS) 65 0 66 0 67 0 68 0 69 0 70 0 71 0 72 0 73 0 74 0 75 0 76 -- 77 0 78 0 79 0 80 -- GROUP 6 (PFOS) 81 0 82 0 83 0 84 0 85 0 86 0 87 0 88 0 89 0 90 0 91 0 92 93 0 94 0 95 0 96 13 . 13 12 17 16 15 ----- 15 11 12 ----- 21 19 16 22 22 19 ------ 21 18 . 17 . . -- 10 21 9 16 10 18 9 ' 16 10 21 14 24 13 24 12 23 14 21 76 12 17 ----- -- 13 17 14 15 13 19 -- ~-- 12 14 14 13 14 11 13 12 14 13 15 ---- 9 12 12 22 24 24 22 25 18 24 19 22 20 19 -- 11 14 17 33 28 25 41 33 34 ---- 38 32 31 -- 32 28 28 28 32 35 36 33 34 19 34 ------ 30 27 28 --- 33 35 35 32 41 29 37 32 37 34 35 -- 22 26 30 35 44 30 41 26 34 45 63 38 52 39 50 ---- 43 57 34 47 34 42 ---- 21 50 34 42 33 42 32 41 34 45 41 52 42 51 38 47 38 52 16 28 35 50 ----- ---- 32 45 28 43 28 38 -- .----- 43 52 39 4 7 41 50 38 46 48 62 31 38 44 54 38 48 43 55 36 51 38 ------ 56 -- 21 29 25 36 30 40 48 52 58 42 49 52 33 39 43 69 82 93 58 65 71 53 58 63 ------ ------ ------ 61 66 73 49 60 67 46 54 60 ------ 56 63 68 43 50 50 44 49 55 45 50 51 45 51 54 57 66 68 52 59 62 49 56 58 55 64 71 30 35 35 56 63 -- ----- 69 49 54 59 47 56 58 43 46 51 -- --- 1 ---- 57 . 49 54 47 67 38 59 52 59 58 63 ---- 32 39 43 63 54 58 56 75 46 67 59 68 65 72 ------ 40 45 48 67 61 63 59 77 51 68 64 74 72 77 --~-- 42 48 52 1 63 BACK TO MAIN NOTOX Project 242933 BODY WEIGHT GAIN (%) MALES BWG-IND - 4 1 7 - M A R - 99 RECOVERY DAYS WEEKS ANIMAL 1 1 5 1 8 12 14 19 22 25 28 22 2 34 44 GROUP 4 i ) 54 55 56 57 58 59 60 61 62 63 ------ -- --- --- -- -- ---- -- 77 101 107 118 121 ---- 58 79 81 89 89 -- -- -- 111 111 coH* 51 70 73 79 --- -- -- -- -- 79 58 77 85 91 87 98 107 104 52 72 78 87 85 95 99 105 106 47 67 72 80 80 87 89 93 92 64 . . . GROUP 5 (PFHS) 65 -- -- -- -- 66 -- -- -- -- 67 -- --- -- -- 68 ' -- -- -- -- 69 -- -- -- -- 70 -- -- -- -- 71 -- -- -- -- 72 -- ------ ------ 73 59 79 86 89 88 74 24 40 45 47 46 -- -- -- -- 75 . 56 74 78 85 83 76 ---- ------ ---- ---- 77 48 67 73 73 75 79 81 90 87 78 45 64 71 74 76 82 84 96 90 79 40 57 60 63 66 69 71 77 75 80 -- - - - --- GROUP S (PFOS) 81 82 83 -- -- -- -- 84 85 86 87 88 89 . 59 84 86 93 90 . 60 79 81 87 93 91 91 66 85 91 99 100 92 93 31 48 52 58 59 94 39 56 59 65 65 95 39 56 60 69 68 96 -- - -- -- -- ::: -- -- 64 68 77 73 76 80 73 78 82 74 82 82 164 BACK TO MAIN NOTOX Project 242933 BODY WEIGHT GAIN (%) FEMALES BWG-IND - 5 1 7 - M AR-99 TREATMENT nAYS WEEKS ANIMAL 1. 1 5 1 8 12 15 19 22 25 2 2334 4 GROUP 1 (VEHICLE CONTROL) 97 ' 98 99 100 101 102 103 104 105 106 . 0 0 0 0 0 0 0 0 0 0 2 4 4 -1 4 3 1 8 7 7 107 0 5 108 109 0 - 1 110 0 7 111 0 4 112 GROUP 2 (TELCMER ALCOHOL) 113 0 4 114 0 5 115 0 1 116 0 4 117 0 6 118 0 4 119 0 3 120 0 10 121 0 4 122 0 5 123 124 _0 _5 125 0 2 126 0 2 127 128 --0 --10 GROUP 3 (TELCMER ACRYLATE) 129 0 5 130 0 5 131 0 0 132 0 3 133 0 7 134 0 5 135 0 6 136 0 2 137 0 4 138 0 6 139 140 _0 ---1 141 0 6 142 0 3 143 144 0 -- --5 GROUP 4 ( 145 0 5 146 0 7 147 0 3 148 0 1 149 0 1 ' 5 9 9 8 14 5 6 12 8 11 8 2 9 7 8 13 7 8 10 5 9 15 3 4 --7 6 1 --1 1 9 8 9 10 8 12 9 10 6 5 --3 10 4 9 -- 8 9 3 5 10 13 19 25 29 32 18 20 27 26 27 18 21 29 28 36 16 10 22 23 27 21 29 35 36 47 9 19 . 23 25 28 10 9 16 23 27 19 24 31 30 39 20 20 27 28 37 21 23 30 31 37 17 16 24 20 31 10 11 15 20 22 21 18 30 27 41 15 17 25 28 32 12 21 25 31 37 21 26 37 40 46 12 14 22 20 29 15 21 27 29 34 17 22 30 27 36 13 19 26 29 35 18 24 29 31 35 19 27 31 29 43 9 9 16 20 28 13 10 18 26 30 --14 17 --- --23 - 26 -- 35 --- 14 15 26 25 . 29 10 12 20 21 27 --23 --22 --33 34 --39-- 13 12 21 20 29 15 14 21 . 24 34 14 18 23 24 27 15 19 21 26 30 15 18 25 25 33 15 15 20 27 36 19 23 31 35 38 16 11 . 20 23 31 15 20 30 35 39 10 9 11 10 17 11 -- --1 1 21 -- 25 -- 28 -- 21 21 30 . 28 32 14 11 19 20 28 17 17 26 25 36 -- ------ 16 17 22 20 30 16 20 29 27 37 13 . 21 28 31 34 15 20 28 29 36 17 17 25 29 30 1 65 28 4 31 32 38 36 47 29 26 41 39 40 35 25 46 32 34 49 31 34 43 32 43 45 28 28 36 -- 33 29 42 32 35 30 32 33 37 38 30 36 20 26 -- 34 28 36 32 38 34 35 35 BACK TO MAIN NOTOX Project 242933 B O D Y W E I G H T G A I N {%) FEMALES * . BWG- IND - 6 17 -MAR-99 RECOVERY CAYS 1 5 8 12 14 19 22 25 WEEKS 1 1 2 2234 4 ANIMAL GROUP 1 (VEHICLE CONTROL) 97 98 99 100 101 102 -- --- -- -- -- -- -- -- --- -- -- -- -- --- . -- -- -------- 103 104 -- -- - -- -- ---- -- -- --- -- -- 105 27 41 41 44 106 27 41 44 47 107 20 31 38 41 108 ---- -- --- ------ 109 13 29 31 34 110 28 45 52 56 1 1 1 21 36 38 42 112 --- -- GROUP 2 (TELOMER ALCOHOL) 113 114 115 116 . 117 118 119 -- -- -- -- -- -- -- -- -- -- -- -- ---- ---- -- -- ---- ---- ---- 120 -- -- -- ---- 121 17 29 32 37 122 18 34 36 42 123 124 125 126 127 128 22 38 39 45 ---- -- -- --- --- 22 39 37 43 18 38 38 43 27 -- 43 -- 48 -- --52 GROUP 3 (TELOMER ACRYLATE) 129 130 131 ---- -- -- --- -- -- -- 132 -- -- -- -- 133 134 ---- ---- -- -- -- -- 135 -- -- -- -- 136 .- -- - -- --- 137 28 48 50 57 138 6 19 19 27 139 15 36 36 45 140 -- -- -- * -- 141 23 37 38 43 142 15 32 35 41 143 21 36 39 46 144 "- -- -- --- GROUP 4 < . 145 146 147 -- -- -- -- -- -- ---- 148 149 -- -- -- . -- ---- -- -- -- --- -- ---- ---- -- ---- --- -- 54 -- 54 -- 44 . -- --- -- 35 36 58 61 42 45 --- -- ---- -- --.. -... ----- ' ... -- 39 63 49 -- -- -- -- -- -- -- ... -- -- ... --- 41 66 51 -- ---- -- . -- ---- ---- ---- -- -- .- ... 33 -- 37 -- 46 -- ... --- 46 50 41 43 --55 --57 ---- ---- ---- -- ------ -- - . -_-- -- _ ... ---- ..- -- ... ... 52 53 46 48 --60 --5-8 -- -- -- -- -- -- -- -- --- ------ ------ -- -- - --- ... ... ... 55 -- _- 26 . -- -- 42 ... ... ... -- - _-- 45 51 55 36 43 43 46 -- 51 -- . --51 -- -... -- --. ... -- -- -- ... 53 46 52 -- -- -- ---- ---- ---- --- -- -- --- --- ---- 1 66 28 4 -- ... -- -- -- -- ... -- -- ... ... 45 72 51 -- -- -- -- -- -- -- ... -- -- -- ... 51 49 . .6.0 -- ... -- ... -- -- --- -- -- --- 51 46 52 ... ... ... ... -- BACK TO MAIN NOTOX Project 242933 BODY WEIGHT GAIN {%) FEMALES - BWG-IND - 7 17-MAR-99 TREATMENT DAYS . WEEKS ANIMAL 1 1 5 1 8 12 15 19 22 25 28 22 33 44 4 GROUP 4 { 150 151 152 153 154 155 ' 156 157 158 159 0 0 0 0 0 0 0 0 0 5 12 24 29 35 36 38 42 4 6 17 21 23 31 39 39 4 10 16 15 25 27 36 39 1 1 9 12 18 24 30 31 8 10 21 19 29 31 42 44 4 6 17 15 22 26 33 34 4 3 15 . 17 27 27 31 31 4 4 17 16 23 21 35 30 7 8 19 23 29 35 39 40 160 -- -- -- -- -- -- ______ ----- -- -- GROUP 5 (PEHS) 161 03 8 15 16 .23 25 27 30 162 05 9 20 18 25 24 31 34 163 03 7 12 9 18 22 28 28 164 01 6 12 13 21 20 27 30 165 0 -1 6 15 22 29 34 36 40 166 0 -1 8 13 17 25 31 37 35 167 0 -2 -1 8 14 20 22 26 27 168 03 6 15 21 26 30 35 35 169 0 7 8 15 15 26 25 31 33 170 04 6 12 11 20 22 30 29 171 0 9 11 21 18 27 32 38 41 172 173 01 3 12 11 15 19 26 23 174 0 6 6 15 17 26 27 35 35 175 0 5 7 15 17 26 24 33 35 176 -- --- -- -- --- -- -- -- -- GROUP 6 (PFOS) 177 0 6 11 20 21 29 32 38 36 178 0 -1 5 12 10 16 16 20 21 179 05 8 12 6 13 17 21 23 180 0 - 1 3 9 10 14 15 18 15 181 0 -2 2 5 7 10 9 1 1 11 182 05 6 12 18 23 22 28 30 183 0 0 7 14 12 17 22 25 22 184 01 9 16 12 21 24 26 29 185 09 7 17 17 31 30 32 27 186 07 7 17 19 25 24 26 28 187 xioa oa 01 7 . 15 13 19 24 28 29 189 01 3 12 11 17 18 21 23 190 04 6 15 14 24 27 32 37 191 0 4 2 9 8 12 10 16 16 192 ---- -- --- -- -- -- *-- -- 1 67 BACK TO MAIN NOTOX Project 242933 BODY WEIGHT GAIN (%) FEMALES SWG-IND - 8 17-MAR-99 RECOVERY DAYS WEEKS ANIMAL 1 1 5 1 8 2 12 2 14 2 19 34 22 4 25 4 28 GROUP 4 ( ) 150 151 -- 152 -- 153 20 154 30 155 23 156 -- -- 157 23 158 22 159 30 160 -- GROUP 5 (PEHS) 161 162 -- 163 ---- 164 -- 165 -- - 166 -- 167 -- 168 -- -- 169 24 170 19 . 171 29 172 -- -- 173 14 174 23 175 23 176 -- ~ GROUP 6 (PPOS) 177 178 -- 179 -- 180 -- 181 -- 182 -- -- 183 -- -- . 184 -- 185 17 186 16 187 17 188 -- -- 189 11 190 25 191 7 192 -- -- ---- 40 45 40 ---- 35 36 38 -- -- -- -- -- -- --- 33 32 45 --- 29 42 38 --- -- -- ---- -- ----- 34 32 32 --- 23 38 17 ---- --. ---- * 44 49 44 -- 40 32 38 -- ---- ---- ... 51 49 54 53 46 44 ---- --- 42 41 37 46 44 43 -- . .-- -- ---- ----. -- .. -- ... 39 48 47 -- --... ... ... -- ... 44 49 51 . ---- ... ... ... ... -- -- 50 51 55 -- ----' ---- -- -- . -- ------ 40 36 46 -- 29 43 42 -- -- -- -- --- -- -- ---- 40 41 54 --- 33 46 48 --- -- -- -- -- -- --. --- 40 39 53 --- 29 45 50 --- -- -- -- --- -- -- __ . ____ -- ... ____ 36 47 53 -- -- ... -- -- -- ... ... ... -- ... ___ 37 50 55 -- -- ... -- -- -- .-- ... -- ... ____ 43 56 56 -- -- -- -- -- -- -- -- 35 36 36 ---- 28 41 21 --- -- -- -- -- -- ... -- 40 37 42 -- 31 45 26 --- -- -- ---- -- 38 37 42 ... 34 45 28 ---- -- -- --- -- .. ... ... ... -- ..... ... 37 52 33 ...- . .-- -- ---- _-- . ... -- ... ... 36 50 31 ... -- -- . ----- .-- ... ... ... -- ... .i-- - 37 52 33 -- ... ... ... ... -- ... 46 48 56 -- -- ... -- ... -- ... ... ... -- ... ____ 42 54 59 ... ---- -- -- ... ____ ... ... -- ... ... 38 55 34 --- 1 68 BACK TO MAIN NOTOX Project 242933 ~ FOOD CONSUMPTION (G/ANIMAL/DAY) MALES FC-IND - 1 01 -MAR- 9 9 CAYS. WEEKS CAGE TREATMENT 1-8 1/2 . 8-15 2/3 15-22 3/4 22-28 4 RECOVERY 1-8 1/2 8-14 2 14-19 2/3 19-22 3/4 GROUP 1 (VEHICLE CONTROL) 1 26 26 27 28 2 27 29 29 31 3 24 25 28 29 4 29 31 34 33 ___ __ __ __ ___ ___ 29 29 _ 32 31 30 31 GROUP 2 (TELCMER ALCOHOL) 5 28 29 30 31 6 28 30 29 31 7 24 25 27 28 8 26 29 30 30 __ __ __ __ __ ___ 27 27 ___ ___ 29 29 28 29 GROUP 3 (TELCMER ACRYLATE) 9 26 27 28 30 _ 10 11 28 29 30 30 26 28 31 32 __ __ __ 29 30 ___ ____ 12 27 28 32 31 32 32 30 32 GROUP 4 ( ) ' 13 27 29 29 30 . 14 27 28 28 29 15 25 29 33 34 16 27 29 32 31 _ _ 33 32 __ _ 31 30 30 31 GROUP 5 (PFHS) 17 26 28 28 29 18 29 30 31 32 19 24 26 30 30 20 24 25 29 28 GROUP 6 (PROS) 21 28 29 28 29 22 28 30 30 31 23 27 30 34 36 24 26 27 29 29 -- ---- 30 29 _ 30 28 ---- 26 ___ 27 _ __ __ __ _____ __ ____ _____ 33 33 -- -- 28 27 27 28 1 69 NOTOX Project 242933 FOOD CONSUMPTION (G/ANIMAL/DAY) MALES DAYS WEEKS CAGE RECOVERY 22-28 '4 GROUP 1 (VEHXCLE CONTROL) 1 ' -2 3-- 4 31 GROUP 2 (TELOMER ALCOHOL) 5-- 6-- 7 --8 28 GROUP 3 (TELOMER ACRYLATE) 9 10 11 12 30 GROUP 4 ( 13 14 15 16 29 GROUP 5 (PFHS) 17 18 19 -- 20 27 GROUP 6 (PFOS) 21 22 -- 23 -- 24 27 BACK TO MAIN FC-IND - 2 0 1 - M A R - 99 1 70 NOTOX Project 242933 FOOD CONSOMPTION (G/ANIMAL/DAY) FEMALES DAYS WEEKS CAGE TREATMENT ' 1-8 1/2 8-15 2/3 15-22 3/4 22-28 4 ' GROUP 1 (VEHICLE CONTROL) 25 18 20 21 22 26 20 21 22 23 27 20 21 23 25 28 19 21 24 25 GROUP 2 (TELOMER ALCOHOL) 29 19 . 21 22 24 30 19 21 21 23 31 17 . 20 22 22 32 18 20 23 23 GROUP 3 (TELOMER ACRYLATE) 33 18 19 20 22 34 21 22 22 24 35 18 20 22 21 36 19 21 23 23 GROUP 4 37 19 20 21 23 38 19 21 22 23 39 20 22 23 24 40 19 22 23 23 GROUP 5 (PEHS) 41 19 19 21 22 42 22 23 24 24 43 19 21 23 23 44 19 22 23 23 GROUP 6 (PPOS) 45 22 20 20 20 46 18 19 19 19 47 17 19 19 19 48 19 20 21 20 BACK TO MAIN FC-IND - 3 0 1 - M A R - 99 RECOVERY 1-8 1/2 8-14 2 14-19 2/3 19-22 3/4 ..... ... __ '... 25 24 25 24 ... ... ___ 22 ... _ _. . . . ... 23 22 ... 24 23 22 _ ... ... ' ... 23 22 __ 24 23 22 __ _ ... ... 25 24 ... 23 22 20 ... ... __ . . . ... 25 24 -_ 24 23 20 _ _ __ _- _ __ 21 20 __ 21 21 19 ... ... ... 24 ... ... 23 ... ... 22 ... ... 21 ... ... .... 22 ... -- ... 19 1 71 NOTOX Project 242933 FOOD CONSOMPTION (G/ANIMAL/DAY) FEMALES RECOVERY DAYS WEEKS CAGE 22-28 4 GROUP 1 (VEHICLE CONTROL) 25 26 27 28 24 GROUP 2 (TELCMER ALCOHOL) 29 -30 -31 -32 22 GROUP 3 (TELOMER ACRYLATE) 33 -34 -35 -36 22 GROUP 4 ( ) 37 38 39 40 22 GROUP 5 (PFHS) 41 42 43 44 21 GROUP 6 (PFOS) 45 46 47 48 - 19 BACK TO MAIN FC-IND - 4 0 1 - M A R - 99 1 72 BACK TO MAIN NOTOX Project 242933 RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) MALES GROUP 1 (VEHICLE CONTROL) DAYS WEEKS CAGE TREATMENT 1-8 1/2 1 110 2 115 3 107 4 126 8-15 2/3 94 102 99 116 15-22 3/4 89 93 99 114 22-28 4 86 90 96 101 DAYS WEEKS CAGE RECOVERY 22-28 4 1 2 3 4 RFC-IND - 1 0 1 - M A R - 99 RECOVERY 1-8 1/2 8-14 2 14-19 . 19-22 2/3 3/4 ._ -_ ___ ---- 89 84 __ __ . 91 84 77 78 1 73 BACK TO MAIN NOTOX Project 242933 RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) MALES GROUP 2 (TELOMER ALCOHOL) RPC-IND - 2 0 1 - M A R - 99 DAYS WEEKS CAGE TREATMENT 1-8 1/2 5 115 6 116 7 109 8 109 8-15 2/3 101 106 98 104 15-22 3/4 93 91 97 101 22-28 4 ' 90 91 93 92 DAYS WEEKS CAGE RECOVERY 22-28 4 5 6 7 8 72 RECOVERY 1-8 1/2 8-14 2 14-19 2/3 19-22 3/4 -- ---- 84 79 85 80 -- -- 74 --- -- 76 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) HALES GROUP 3 (TELOMER ACRYLATE) DAYS WEEKS CAGE TREATMENT 1-8 1/2 9 112 10 119 11 111 12 116 8-15 2/3 101 105 103 105 15-22 3/4 95 95 103 105 22-28 4 93 89 97 95 DAYS WEEKS CAGE RECOVERY 22-28 4 9 -- 10 11 12 74 BACK TO MAIN RFC-IND - 3 0 1 - M A R - 99 RECOVERY 1-8 1/2 8-14 2 14-19 2/3 19-22 3/4 -- 83 90 -- 80 84 ----- -- 76 -- -- 80 1 75 NOTOX Project 242933 RELATIVE FOOD CONSOMPTIQN (G/KG BODY WEIGHT/DAY) MALES GROUP 4 ( ) DAYS WEEKS CAGE TREATMENT 1-8 1/2 13-> 113 14 112 15 107 16 1 1 1 8-15 2/3 103 102 105 99 15-22 3/4 91 93 104 98 22-28 4 90 90 100 89 DAYS WEEKS CASE RECOVERY 22-28 4 13 --14 --15 -16 67 BACK TO MAIN RFC-IND - 4 0 1 - M A R - 99 RECOVERY ' 1-8 1/2 8-14 2 14-19 2/3 .19-22 3/4 ... -- --- ---. -- 88 82 ---- -- 82 73 71 72 1 76 NOTOX Project 242933 RELATIVE FOOD CONSDMPTION (G/KG BODY WEIGHT/DAY) MALES GROUP 5 (PFHS) DAYS WEEKS CAGE TREATMENT 1-8 1/2 8-15 2/3 17 110 101 18 122 105 19 106 99 20 108 100 15-22 3/4 91 97 102 102 22-28 4 88 95 96 94 DAYS WEEKS CAGE RECOVERY 22-28 4 17 --18 -19 -20 73 BACK TO MAIN RFC-IND - 5 0 1 - M AR-99 RECOVERY 1-8 1/2 8-14 ' 14-19 2 2/3 19-22 3/4 .-- -- --- -- ---. _ 91 83 '-- 92 - 83 76 77 1 77 NOTOX Project 242933 _ ~ RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) MALES GROUP 6 (PFOS) DAYS WEEKS CAGE x s e a s m o t t 1-8 1/2 21 120 22 114 23 1 1 1 24 113 8-15 2/3 106 102 105 103 15-22 3/4 22-28 4 94 , 91 104 103 92 87 100 99 DAYS WEEKS CAGE RECOVERY 22-28 4 21 22 23 --24 72 BACK TO MAIN RFC-IND - 6 01-MAR-99 RECOVERY 1-8 1/2 8-14 2 14-19 2/3 19-22 3/4 -- --. -- 85 80 -- ____ 88 81 76 78 1 78 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES GROUP 1 (VEHICLE CONTROL) DAYS WEEKS CAGE TREATMENT 1-8 1/2 25 106 26 105 27 107 28 107 8-15 2/3 104 102 103 107 15-22 3/4 98 97 104 112 22-28 4 101 94 104 109 DAYS WEEKS CAGE RECOVERY 22-28 4 25 26 27 28 . ---- 91 BACK TO MAIN RFC-IND - 7 0 1 - M A R - 99 RECOVERY 1-8 1/2 8-14 2 14-19 2/3 19-22 3/4 .------ --- 102 -- 95 -- --- -- -- 107 95 86 92 . 1 79 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES GROUP 2 (TELOMER ALCOHOL) DAYS WEEKS CAGE TREATMENT 1-8 1/2 29 104 30 110 31 96 32 105 8-15 2/3 102 108 105 106 15-22 3/4 98 100 110 111 22-28 4 97 102 101 108 DAYS WEEKS CAGE RECOVERY 22-28 4 29 --30 -- 31 --32 86 BACK TO MAIN RFC-IND - 8 0 1 - M A R - 99 RECOVERY 1-8 1/2 8-14 2 14-19 2/3 19-22 3/4 __ ___ 102 93 106 98 88 92 1 80 NOTOX Project 242933 - RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES GROUP 3 (TELOMER ACRYLATE) . TREATMENT DAYS WEEKS CAGE 1-8 1/2 8-15 . 15-22 2/3 3/4 33 105 100 100 34 117 110 107 35 108 110 112 36 110 109 112 22-28 4 101 107 . 105 107 AYS WEEKS CAGE RECOVERY 22-28 '4 ' 33 --34 --35 --36 90 BACK TO MAIN RFC-IND - 9 01-MAR-99 * . RECOVERY 1-8 1/2 8-14 2 14-19 2/3 19-22 3/4 -- -- __ _ 105 96 ---- 109 98 88 87 1 81 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES GROUP 4 ( ) DAYS WEEKS CASE TREMMENT 1-8 1/2 37 104 38 110 39 115 40 104 8-15 2/3 104 102 113 107 15-22 3/4 98 102 112 106 22-28 4 100 100 106 100 DAYS WEEKS CASE RECOVERY 22-28 4 ' 37 -38 -39 --40 81 ' BACK TO MAIN R F C - I N D - 10 0 1 - M A R - 99 RECOVERY 1-8 1/2 8-14 2 14-19. 2/3 19-22 3/4 -------- 106 98 -- ----- --- 97 -- -- 91 81 83 1 82 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION {G/KG BODY WEIGHT/DAY) FEMALES GROUP 5 (PFHS) DAYS WEEKS CAGE TBSKTMBSV 1-8 1/2 41 110 42 130 43 102 44 109 8-15 2/3 100 118 105 113 15-22 3/4 100 111 107 111 22-28 4 103 105 102 106 DAYS WEEKS CAGE RECOVERY 22-28 4 41 -- 42 -43 -44 84 BACK TO MAIN RFC-IND - 11 0 1 - M A R - 99 RECOVERY 1-8 . 1/2 8-14 2 14-19 2/3 19-22 3/4 -- __ ___ ___ 107 97 105 98 84 88 1 83 NOTOX Project 242933 RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES GROUP 6 (PFOS) DAYS WEEKS CAGE TREATMENT 1-8 1/2 45 46 47 48 125 104 100 115 8-15 2/3 106 97 100 111 15-22 3/4 101 92 96 110 22-28 4 95 92 93 102 DAYS WEEKS CAGE RECOVERY 22-28 4 45 -46 -47 -48 85 BACK TO MAIN RFC-IND - 12 0 1 - M A R - 99 RECOVERY 1-8 1/2 8-14 2 14-19 2/3 19-22 3/4 . . . __ __ 97 90 103 95 85 86 184 BACK TO MAIN NOTOX Project 242933 ` TELOMER ALCOHOL/TELOMER ACRYLATE/ 7PFHS/PF0S KEY-IND - 1 22-MAR-99 KEY TO MISSING VALUES AND REMARKS HAEMATOLOGY / CLINICAL BIOCHEMISTRY AFTER 4 WEEKS Animal 1, 2, 3, 4, 56 81, 84, 97, 98, 99, 114, 129, 145, 164, 177, 179, 180, Animal 1, 33, 55, 59, 65, 72, 77, 78. 79, 89, 132, 145, 162, 168, Animal 62, 174 Animal 129 --- = EDTA sample clotted -- = Citrate sample clotted = PTT in plasma not reproducible = deviating curve noted for PTT 1n plasma Animal 2, 17, 18, 19, 25, 36, 40, 49, 50, 66, 67, 71, 72, 74, 79, 87, 88, 89, 90 Animal 46 and 59 Serum sample showed haemolysis = ALAT in serum not reproducible AFTER 2 WEEKS RECOVERY Animal 75, 169, 186 AFTER 4 WEEKS RECOVERY Animal 14, 29, 61, 78 Animal 127 . Citrate sample clotted = Citrate sample (partly) clotted --- = ALAT in serum not reproducible NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN HEM-IND - 1 30-MAR-9 9 ANIMAL NUMBER HAEMATOLOGY PARAMETERS RBC HB HCT T/l Bmol/1 1/1 1 2 3 4 5 7.52 6 6.83 7 7.25 8 7.01 9 6.85 10 7.11 11 6.91 13 6.97 14 .7.29 15 6.80 9.7 9.2 9 .-6 9.4 8.9 9.0 9.0 8.6 9.2 8.7 0.423 0.406 0.415 0.411 0.403 0.413 0.406 0.390 0.418 0.389 mcv 1 56.3 59.4 57.2 58.6 58.8 58.1 58.8 56.0 57.3 57.2 MCH fmol MCHC itmol/1 REW % 1.290 1.347 1.324 1.341 1.299 1.266 1.302 1.234 1.262 1.279 22.9 22.7 23.1 22.9 22.1 21.8 22.2 22.1 22.0 22.4 12.3 12.2 12.2 11.6 12.4 12.4 12.4 13.4 12.3 12.5 PLATELETS G/l 1329 1182 1340 1085 1237 988 1187 1074 1189 1204 ANIMAL NUMBER 1 2 3 4 5 6 7 8 9 10 11 13 14 15 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 BASO. 1 -- -- 10.9 17.6 14.1 16.2 8.8 6.6 9.9 10.9 9.3 9.0 -- -- -- 0.105 0.140 0.075 0.090 0.190 0.145 0.110 0.145 0.120 0.120 -- -- -- 0.010 0.010 0.000 0.005 0.015 0.005 0.010 0.000 0.010 0.005 -- -- --- 0.000 0.000 0.000 . 0.000 0.000 0.000 0.000 0.000 0.000 0.000 MONO. 1 -- -- ... 0.025 0.030 0.030 0.030 0.010 0.020 0.030 0.025 0.030 0.025 LYMPH. 1 -- --- 0.860 0.820 0.895 0.875 0.785 0.830 0.850 0.830 0.840 0.850 FT SEC 15.5 15.6 14.7 14.2 15.2 14.3 14.5 14.7 14.8 14.9 14.3 16.2 14.7 FTT SEC 15.5 16.2 17.2 16.7 16.3 ' 17.5 16.1 14.9 16.0 15.7 15.7 14.8 16.4 NOTOX Project 242933 HAEMATOLOGY . AFTER 4 WEEKS MALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN HEM-IND - 2 3 0 - M A R - 99 ANIMAL NUMBER 17 ia 19 20 21 22 23 24 25 26 27 29 30 31 HAEMATOLOGY PARAMETERS RBC HB HCT T/l irmol/1 1/1 6.19 8.3 0.386 6.36 8.5 .0.371 6.21 8.4 0.368 6.49 8.2 0.377 6.70 9.0 0.403 6.76 8.9 0.391 7.09 9.2 0.405 6.93 9.2 0.407 6.83 8.9 0.399 6.93 9.1 0.411 7.02 8.9 0.404 7.21 9.5 0.425 6.51 8.7 0.389 6.80 8.6 0.391 mcv fl 62.4 58.3 59.3 58.1 60.1 57.8 57.1 58.7 58.4 59.3 57.5 58.9 59.8 57.5 MOT fmol 1.341 1.336 1.353 1.263 1.343 1.317 1.298 1.328 1.303 1.313 1.268 1.318 1.336 1.265 M2HC imol/1 21.5 22.9 22.8 21.8 22.3 22.8 22.7 22.6 22.3 22.1 22.0 22.4 22.4 22.0 ROM % 10.7 11.5 12.4 11.2 12.0 12.2 1 1 .1 12.4 12.5 12.7 11.7 12.7 13.0 11.7 PLATELETS G/l 842 928 929 913 1111 1216 992 1047 1119 897 984 1051 1140 1184 ANIMAL NUMBER 17 18 19 20 21 22 23 24 25 26 27 29 . 30 31 HAEMATOLOGY PARAMETERS NBC SB3. BO. G/l 1 1 10 .1 7.2 6.4 7.3 10.4 12.3 8.6 10.9 10.9 11.7 10.5 8.6 12.5 11.9 0.085 0.085 0.190 0.155 0.105 0.115 0.120 0.080 - 0.130 0.105 0.100 0.225 0.130 0.130 0.000 0.025 0.005 0.015 0.000 0.015 0.005 0.010 0.015 0.040 0.000 0.010 0.015 0.005 BASO. 1 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 MONO. 1 0.015 0.015 0.015 0.040 0.015 0.040 0.020 0.010 0.025 0.005 0.035 0.040 0.015 0.015 LYMPH. 1 PT SEC 0.900 0.875 0.790 0.790 0.880 0.830 0.855 0.900 0.830 0.850 0.865 0.725 0.840 0.850 15.8 14.8 ' 15.6 14.5 14.4 14.5 15.0 15.4 13.9 15.3 14.8 15.7 15.3 14.5 PTT SEC 16.0 14.9 15.4 16.4 15.8 16.9 14.6 16.6 15.5 16.5 16.2 17.1 15.9 15.8 ' 1 87 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN HSM-IND - 3 3 0 - M A R -9 9 ANIMAL NUMBER 33 34 35 36 37 38 39 40 41 42 43 45 46 47 HAEMATOLOGY PARAMETERS RBC HB HOT T/l ntnol/1 1/1 6.12 6.50 6.36 6.43 6.76 6.83 6.55 7.16 6.49 6.34 6.83 6.73 6.56 6.82 8.2 8.5 8.0 8.2 9.0 9.2 8.5 9.4 8.7 8.3 9.0 8.9 8.9 9.0 0.367 0.382 0.372 0.380 0.397 0.402 0.372 0.409 0.387 0.374 0.409 0.386 0.390 0.403 rocv fl 60.0 58.8 58.5 59.1 58.7 58.9 56.8 57.1 59.6 59.0 59.9 57.4 59.5 59.1 MCH fmol 1.340 1.308 1.258 1.275 1.331 1.347 1.298 1.313 1.341 1.309 1.318 1.322 1.357 1.320 MCHC tnnol/1 22.3 22.3 21.5 21.6 22.7 22.9 22.8 23.0 22.5 22.2 22.0 23.1 22.8 22.3 REW % 12.2 11.5 11.5 11.5 12.7 12.3 12.5 11.6 13.1 12.4 11.8 1 2 .1 1 2 .1 13.4 PLATELETS G/l 1244 957 1063 930 1388 1354 1191 1114 1099 1137 944 1268 904 1022 ANIMAL NMBER 33 34 35 36 37 38 39 40 41 42 43 45 46 47 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 11.8 9.9 9.1 11.2 13.4 9.4 9.6 9.3 11.2 11.8 8.9 13.8 6.7 6.8 0.170 0.155 0.080 0.140 0.100 0.065 0.090 0.080 0.095 0.090 0.120 0.145 0.155 0.150 0.005 0.010 0.000 0.015 0.005 0.005 0.020 0.005 0.005 0.000 0.005 0.005 0.005 0.010 BASO. 1 tONO. 1 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 ' 0.025 0.020 0.015 0.020 0.020 0.010 0.020 0.020 0.015 0.025 0.020 0.020 0.045 0.000 LYMPH. 1 0.800 0.815 0.905 0.825 0.875 0.920 0.870 0.895 0.885 0.885 0.855 0.830 0.795 0.840 PT SEC 15.6 15.0 15.3 13.9 14.3 14.7 15.6 15.1 13.9 15.5 15.5 14.4 14.8 PIT SEC 15.5 17.4 23.8 17.1 16.5 18.7 15.5 16.2 15.9 16.7 15.3 16.1 17.8 1 88 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 4 ( ) . BACK TO MAIN HEM-IND - 4 3 0-MAR-99 .. : . ANIMAL NUMBER 49 50 51 52 53 54 55 57 58 59 61 62 63 HAEMATOLOGY PARAMETERS RBC HB HOT T/l nntol/1 1/1 mcv fl 7.07 6.54 6.25 6.62 6.65 7.18 7.13 8.7 8.2 7.6 8.5 8.7 9.6 9.4 0.406 0.371 0.358 0.387 0.384 0.429 0.418 57.4 56.7 57.3 58.5 57.7 59.7 58.6 6.42 8.7 0.386 60.1 6.48 8.7 0.389 60.0 7.21 9.2 .0.418 58.0 6.82 8.9 0.402 58.9 6.94 9.4 0.418 60.2 6.90 8.8 0.395 57.2 MCH fmol 1.231 1.254 1.216 1.284 1.308 1.337 1.318 1.355 1.343 1.276 1.305 1.354 1.275 MCHC irmol/1 21.4 22.1 21.2 22.0 22.7 22.4 22.5 22.5 22.4 22.0 22.1 22.5 22.3 RDW 11.7 11.5 11.7 11.7 11.9 11.7 1 2 .1 12.2 11.9 12.5 12.4 1 2 .1 1 2 .1 PLATELETS G/l 1063 1149 1030 1072 1462 874 876 1084 1084 860 . 1049 932 1121 ANIMAL NUMBER 49 50 51 52 53 54 55 56 57 58 59 61 62 63 HAEMATOLOGY PARAMETERS WBC SEG. ED. G/l 1 1 BASO. 1 MEMO. 1 12.4 15.2 14.3 14.5 10.6 8.2 10.8 -- 14.1 10.4 12.2 9.6 11.7 7.6 . 0.100 0.105 0.070 0.085 0.065 0.055 -0-.-110 0.095 0.100 0.075 0.055 0.140 0.070 0.010 0.005 0.000 0.010 0.010 0.005 0.010 --- 0.000 0.010 0.010 0.005 0.005 0.015 0.000 0.000 0.000 0.000 0.000 0.000 0.000 -- 0.000 0.000 0.000 0.000 0.000 0.000 0.025 0.020 0.020 0.010 0.015 . 0.020 ' .040 --- . " 0.035 0.015 0.030 0.020 0.035 0.015 LYMPH. 1 0.865 0.870 0.910 0.895 0.910 0.920 0.840 --0.870 0.875 0.885 0.920 0.820 0.900 ET SEC 14.9 14.9 13.7 15.4 15.2 14.1 -- 13.8 13.8 13.8 --14.8 14.1 14.5 PIT SEC 15.7 15.2 16.0 19.0 17.4 19.5 -- 26.8 16.7 16.7 -- 19.5 -- . 18.1 1 89 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 5 (PFHS) BACK TO MAIN HEM-IND - 5 30-MAR-9 9 ANIMAL NUMBER HAEMATOLOGY PARAMETERS RBC KB HCT T/l mmol/1 1/1 65 66 67 68 69 70 71 72 73 74 75 77 78 79 6.14 5.71 5.55 6.50 7.04 7.06 7.47 6.40 6.64 6.72 6.65 '6.35 6.95 6.80 8.0 7.5 7.6 8.1 9.1 9.0 9.7 8.4 8.5 8.7 8.5 8.5 8.6 9.0 0.357 0.347 0.347 0.365 0.395 0.409 0.442 0.371 0.388 0.388 0.380 0.378 0.395 0.404 mcv fl 58.1 60.8 62.5 56.2 56.1 57.9 59.2 58.0 58.4 57.7 57.1 59.5 56.8 59.4 MCH fmol 1.303 1.313 1.369 1.246 1.293 1.275 1.299 1.312 1.280 1.295 1.278 1.339 1.237 1.324 M2HC itmol/1 22.4 21.6 21.9 22.2 23.0 22.0 21.9 22.6 21.9 22.4 22.4 22.5 21.8 22.3 ROT % 11.8 1 1 .1 13.1 11.0 1 1 .1 11.6 12.2 11.8 12.6 12.3 12.0 12.8 12.2 12.8 PLATELETS G/l 1017 929 946 969 1074 977 1011 873 997 943 1092 1031 221 105 ANIMAL NUMBER 65 66 67 68 69 70 71 72 73 74 75 77 78 79 HAEMATOLOGY PARAMETERS WBC SBG. BO. G/l 1 1 BASO. 1 H3NO. 1 7.3 8.1 11.1 9.2 7.1 13.6 14.2 1 2 .1 13.9 8.7 6.4 11.0 6,0 6.5 0.175 0.255 0.185 0.125 0.085 0.170 0.140 0.115 0.220 0.100 0.130 0.105 0.160 0.105 0.005 0.000 0.020 0.000 0.010 0.000 0.000 0.000 0.000 0.000 0.010 ' 0.000 0.000 0.000 0.010 0.000 0.005 0.000 0.020 0.000 0.000 0.000 0.015 0.000 0.005 0.000 0.015 0.000 0.030 0.020 0.015 0.035 0.020 0.025 0.040 0.025 0.050 0.025 0.025 0.040 0.005 0.005 LYMPH. 1 0.790 0.705 0.790 0.840 0.895 0.795 0.820 0.850 0.725 0.855 0.845 0.840 0.830 0.875 PT SEC 13.9 14.4 14.1 14.1 13.5 1--4-.-7 14.7 13.7 1--3-.7 --- -- PIT SEC 15.9 15.8 15.8 17.1 17.4 18.1 -- 17.3 17.4. .1-6-.7 -- -- . 190 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 6 (PFOS) BACK TO MAIN HEM-IND - 6 30-MAR-9 9 ANIMAL NUMBER 81 82 83 84 85 86 87 88 89 90 91 93 94 95 HAEMATOLOGY PARAMETERS RBC HB HOT T/l nmol/l 1/1 -- 6.44 6.20 -- 6.36 7.37 6.95 6.15 6.44 6.48 6.55 7.13 7.02 6.82 -- 8.7 8.2 -- 8.9 9.4 9.6 8.3 9.0 8.7 8.9 8.9 9.1 9.1 0.377 0.361 0.395 0.429 0.414 0.367 0.395 0.382 0.390 0.389 0.409 0.398 mcv fl 58.5 58.2 62.1 58.2 59.6 59.7 61.3 59.0 59.5 54.6 58.3 58.4 MCH fmol M2HC `mnol/1 ROW % PLATELETS G/l 1.351 1.323 1.399 1.275 1.381 1.350 1.398 1.343 1.359 1.248 1.296 1.334 23.1 22.7 22.5 21.9 23.2 22.6 22.8 22.8 22.8 22.9 22.2 22.9 11.3 11.5 11.2 12.7 11.3 13.7 11.5 12.3 12.2 12.7 11.8 11.5 1120 1150 1031 935 1109 1239 1019 1114 1111 1127 ' 1071 1082 ANIMAL NUMBER 81 82 83 84 85 86 87 88 89 90 91 93 94 95 HAEMATOLOGY PARAMETERS WBC SEE. EO. G/l 1 1 7.5 -1-0-.2 11.3 10.8 12.3 8.4 13.2 14.2 10.4 7.4 10.2 12.9 ___ 0.110 0.095 --- 0.075 0.165 0.075 0.190 0.080 0.145 0.075 0.095 0.115 0.145 ___ 0.000 0.010 -- 0.015 0.005 0.005 0.005 0.005 0.005 0.000 0.005 0.000 0.000 BASO. 1 -- 0.000 0.000 -- 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 MONO. 1 0.025 0.020 0.035 0.010 0.015 0.025 0.015 0.035 0.010 0.030 0.025 0.035 LYMPH. 1 0.865 0.875 -- * 0.875 0.820 0.905 0.780 0.900 0.815 0.915 0.870 0.860 0.820 FT SEC 14.1 14.8 15.6 15.4 14.7 14.4 14.5' 14.1 --15.6 14.7 14.6 14.5 13.8 PTT SEC 20.8 15.2 20.0 15.0 16.9 16.1 19.2 15.9 -- ' 17.1 16.4 18.0 17.7 15.7 1 91 N O T O X Project 242933. HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN HEM-IND - 7 3 0 - M A R - 99 ANIMAL NUMBER 97 98 99 100 101 102 103 104 105 106 107 109 110 111 HAEMATOLOGY PARAMETERS RBC HB HCT T/l nmol/1 1/1 -- -- -- 6.50 6.66 6.80 6.82 7.02 6.78 6.79 6.68 6.54 6.63 6.73 -- -- -- 8.9 9.5 9.0 9.2 9.6 8.9 9.2 8.7 8.7 9.0 9.1 0.376 0.292 0.369 0.379 0.389 0.363 0.389 0.369 0.356 0.392 0.380 rocv fl 57.8 58.9 54.3 55.6 55.4 53.5 57.3 55.2 54.4 59.1 56.5 MCH fmol III 1.369 1.426 1.324 1.349 1.368 1.313 1.355 1.302 1.330 1.357 1.352 M2HC mrrol/l 23.7 24.2 24.4 24.3 24.7 24.5 23.7 23.6 24.4 23.0 23.9 ROW PLATELETS % G/l " 10.9 11.2 11.5 11.1 11.6 11.5 10.9 11.9 11.4 11.6 10.5 1271 1114 1324 1050 1302 1335 1078 1214 1019 996 ' 1121 ANIMAL NUMBER 97 98 99 100 101 102 103 104 105 106 107 109 110 111 HAEMATOLOGY PARAMETERS NBC SEG. EO. G/l 1 1 ... -- -- 7.0 11.1 7.0 9.8 9.5 9.0 6.3 4.9 5.2 5.9 5.5 -- ~ -- 0.080 0.060 0.070 0.045 0.070 0.060 0.090 0.130 0.075 0.095 0.125 ___ -- ---- -- 0.015 0.000 0.010 0.005 0.015 0.010 0.005 0.025 0.010 0.010 0.000 BASO. 1 ___ -- ---- -- 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 MONO. 1 ___ -- ... 0.020 0.040 0.045 0.010 0.020 0.015 0.020 0.040 0.025 0.025 0.020 LYMPH. 1 -- ------ 0.885 0.900 0.875 0.940 0.895 0.915 0.885 0.805 0.890 0.870 0.855 PT SEC 14.2 13.9 13.9 14.3 16.5 17.3 15.6 15.5 16.0 15.3 15.9 16.0 15.1 15.4 FIT SEC 14.2 17.7 16.1 18.2 17.0 14.1 17.5 16.3 17.0 16.1 14.1 15.5 14.6 14.7 ; NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN HEM-IND - 8 3 0 - M A R - 99 ANIMAL NUMBER 113 115 116 117 118 119 120 121 122 123 125 126 127 HAEMATOLOGY PARAMETERS RBC HB HCT T/l mraol/l 1/1 . mcv fl 5.44 5.59 6.52 6.28 6.76 6.51 6.21 6.00 6.61 6.29 6.50 6.55 6.29 7.2 7.7 8.7 8.9 9.2 9.0 8.6 8.3 8.6 8.2 8.7 8.8 8.7 0.312 0.331 0.376 0.362 0.372 0.365 0.353 0.340 0.356 0.348 0.363 0.369 0.367 57.4 59.2 57.7 57.6 55.0 56.1 56.8 56.7 53.9 55.3 55.8 56.3 . 58.3 MCH mol 1.324 1.377 1.334 1.417 1.361 1.382 1.385 1.383 1.301 1.304 1.338 1.344 1.383 MCHC . nrnol/1 23.1 23.3 23.1 24.6 24.7 24.7 24.4 24.4 24.2 23.6 24.0 23.8 23.7 REW % 10.8 10.8 11.2 11.2 11.2 10.7 11.2 11.0 11.8 11.6 1 2 .1 11.9 10.7 PLATELETS G/l 1041 1114 970 1134 1463 1275 1262 1283 1097 1016 ' 1165 1095 920 A N IM AL NUMBER 113 114 115 116 117 118 119 120 121 122 123 125 126 127 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 8.9 0.135 0.000 ------ 6.9 0.110 0.020 8.8 0.040 0.000 5.6 0.175 0.005 8.5 0.060 0.005 6.8 0.105 0.005 8.3 0.160 0.005 9.8 0.075 0.005 4.2 0.115 0.000 8.6 0.140 0.000 7.8 0.065 0.000 5.4 0.085 0.005 8.2 0.070 0.000 BASO. 1 0.000 -- 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 MONO. 1 0.000 -- 0.035 0.010 0.025 0.040 0.010 0.025 0.025 0.015 0.020 0.025 0.025 0.010 LYMPH. 1 0.865 -- 0.835 0.950 0.795 0.895 0.880 0.810 0.895 0.870 0.840 0.910 0.885 0.920 PT SEC 13.8 14.6 14.4 14.1 15.3 15.6 15.8 15.3 15.1 15.2 14.7 15.8 15.3 15.6 PTT SEC 16.2 16.4 14.5 15.2 15.5 16.4 15.0 15.1 13.5 15.0 14.9 13.8 14.6 14.2 1 S3 NOTOX Project 242933 - HAEMATOLOGY . AFTER 4 WEEKS FEMALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN HEM-IND - 9 30-MAR -99 . ANIMAL NUMBER 129 130 131 132 133 134 135 136 137 138 139 141 142 143 HAEMATOLOGY PARAMETERS RBC HB HOT T/l imol/l 1/1 7.23 5.98 6.30 6.69 6.36 6.82 6.48 6.54 6.70 6.90 6.83 6.86 6.22 9.4 7.5 8.1 8.9 8.9 9.6 8.7 9.0 8.8 8.8 8.9 9.0 8.6 0.408 0.327 0.360 0.372 0.370 0.388 0.359 0.379 0.362 0.373 0:366 0.378 0.363 mcv fl 56.4 54.7 57.1 55.6 58.2 56.9 55.4 58.0 54.0 54.1 53.6 55.1 58.4 MCH fimi . . ' \ MCHC imol/1 -- 1.300 1.254 1.286 1.330 1.399 1.408 1.343 1.376 1.313 1.275 1.303 1.312 1.383 . 23.0 22.9 22.5 23.9 24.1 24.7 24.2 23.7 24.3 23.6 24.3 23.8 23.7 RDM % 11.3 12.0 11.0 11.5 10.9 10.7 10.8 10.5 12.2 12.6 11.5 11.6 11.1 PLATELETS G/l 1136 1004 1089 1082 1383 1304 1180 1062 1247 1068 1317 1040 1029 ANIMAL NUMBER HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 129 130 5.3 131 6.4 132 6.8 133 . 5.7 134 8.2 135 6.6 136 5.4 137 8.2 138 6.4 139 4.2 141 13.2 142 7.9 143 4.7 0.130 0.195 0.070 0.085 0.110 0.110 0.075 0.095 0.090 0.095 0.120 0.070 0.175 -- 0.005 0.005 0.000 0.000 0.000 0.015 0.005 0.030 0.020 0.015 0.005 0.015 0.005 BASO. 1 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0 .000 MONO. 1 0.025 0.015 0.020 0.015 0.005 0.015 0.010 0.030 0.025 0.000 0.030 0.015 0.015 LYMPH. 1 0.840 0.785 0.910 0.900 0.885 0.860 0.910 0.845 0.865 0.890 0.845 0.900 0.805 PT SEC 14.7 13.8 13.7 -- 14.4 15.6 15.9 15.9 16.0 14.9 15.4 15.3 15.5 14.1 PTT SEC 16.0 16.3 -- . 17.1 15.5 16.4 16.0 16.3 14.7 14.7 14.7 14.6 15.6 1 S4 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 4 ( ) --- ' BACK TO MAIN HEM-IND - 10 3 0-MAR-99 * ANIMAL NUMBER 145 146 147 148 149 150 151 152 153 154 155 157 158 159 HAEMATOLOGY PARAMETERS RBC HB HOT T/l mnol/1 1/1 6.23 6.64 6.45 6.66 6.07 6.86 6.64 6.79 6.99 6.22 7.33 6.86 6.33 8.9 9.0 8.7 9.6 8.4 9.4 9.2 8.7 9.4 8.6 9.4 8.9 9.0 0.380 0.388 0.363 0.392 0.347 0.385 0.383 0.378 0.382 0.363 0.402 0.375 0.368 mcv MCH fl fmol 61.0 58.4 56.3 58.9 57.2 56.1 57.7 55.7 54.6 58.4 54.8 54.7 58.1 . 1.429 1.355 1.349 1.441 1.384 1.370 1.386 1.281 1.345 1.383 1.282 1.297 1.422 MCHC nraol/l RLW % 23.4 23.2 24.0 24.5 24.2 24.4 24.0 23.0 24.6 23.7 23.4 23.7 24.5 11.5 11.5 11.5 11.3 11.3 11.4 10.5 11.9 11.4 11.0 11.4 11.3 11.0 PLATELETS G/l 1194 1032 1102 1172 1276 1134 1136 1137 1177 1169 1176 1332 1165 ANIMAL NUMBER 145 146 147 148 149 150 151 152 153 154 155 157 158 159 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 1 0 .1 7.1 9.1 8.3 4.9 9.8 6.5 5.8 7.8 4.7 8.9 9.0 6.9 0.075 0.080 0.075 0.090 0.065 0.075 0.080 0.145 0.105 0.125 0.070 0.070 0.080 0.005 0.005 0.005 0.010 0.020 0.005 0.010 0.015 0.015 0.015 0.020 0.005 0.015 BASO. 1 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 MONO. 1 0.020 0.015 0.030 0.010 0.025 0.035 0.025 0.025 0.030 0.040 0.025 0.020 0.015 LYMPH. 1 PT SEC 0.900 0.900 0.890 0.890 0.890 0.885 0.885 0.815 0.850 0.820 0.885 0.905 0.890 14.3 14.8 13.5 14.5 14.4 14.7 14.9 14.6 14.4 13.8 15.5 14.4 14.6 PIT SEC 16.8 16.6 16.4 17.5 17.5 18.2 17.6 16.2 15.5 15.7 16.6 17.4 18.7 1 95 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 5 (PFHS) - . BACK TO MAIN HEM-IND - 11 30-MAR-99 . ANIMAL CLUMBER HAEMATOLOGY PARAMETERS RBC HB HOT T/l nmol/1 1/1 161 5.92 162 6.55 163 . 6.70 165 6.23 166 6.58 167 6.65 168 6.36 169 6.07 170 6.42 171 6.50 173 6.63 174 5.94 175 6.02 8.4 9.0 8.7 8.6 9.0 8.8 9.1 8.5 8.4 9.1 8.5 8.7 7.8 0.356 0.375 0.366 0.353 0.374 0.360 0.374 0.333 0.342 0.381 0.350 0.359 0.325 mcv fl 60.1 57.3 54.6 56.7 56.8 54.1 58.8 54.9 53.3 58.6 52.8 60.4 54.0 MCH fmol ' 1.419 1.374 1.299 1.380 1.368 1.323 1.431 1.400 1.308 1.400 1.282 1.343 1.296 MCHC ntnol/1 REW % 23.6 24.0 23.8 24.4 24.1 24.4 24.3 25.5 24.6 23.9 24.3 24.8 24.0 10.9 10.9 11.8 11.7 11.0 11.9 10.6 11.3 10.9 10.7 11.6 10.6 12.7 PLATELETS G/l 1112 314 975 1192 1178 1044 1120 1053 1066 1157 1019 938 1281 ANIMAL NUMBER 161 162 163 164 165 166 167 168 169 170 171 173 174 175 HAEMATOLOGY PARAMETERS WBC SEG. BO. G/l 1 1 6.3 4.8 5.2 -- 7.2 10.8 5.8 9.1 4.6 6.7 5.5 4.5 4M 7.2 0.065 0.050 0-.-065 0.040 0.040 0.090 0.065 0.115 0.110 0.045 0.095 0.155 0.100 0.005 0.005 0.015 -- 0.010 0.010 0.020 0.005 0.005 0.010 0.005 0.020 0.005 0.005 BASO. 1 0.000 0.000 0.000 -- 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 MONO. 1 0.020 0.010 0.005 -- 0.045 0.035 0.035 .015 0.035 0.015 0.020 0.025 0.035 0.020 LYMPH. 1 0.910 0.935 0.915 -- 0.905 0.915 0.855 0.915 0.845 0.865 0.930 0.860 0.805 0.875 PT SEC 13.7 -- 14.0 14.3 14.1 13.9 15.8 -- 15.9 14.4 14.2 14.4 15.1 14.0 PTT SEC 15.8 * ----- 15.7 15.1 16.4 17.9 2 2 .1 -- 16.0 15.5 18.1 1-5-.9 16.8 . ' 1 96 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 6 (PFOS) BACK TO MAIN HEM-IND - 12 3 0 - M A R - 99 ANIMAL NUMBER 177 178 179 180 181 182 183 184 185 186 187 189 190 191 HAEMATOLOGY PARAMETERS RBC HB HOT T/l tnnol/1 1/1 mcv fl 6.69 6.54 6.30 7.23 6.51 6.38 5.98 6.66 6.07 5.95 6.21 8.6 8.9 8.6 9.6 9.0 8.5 8.0 8.9 8.1 8.6 8.2 0.360 53.8 0.369 0.356 0.399 0.369 0.354 0.334 0.371 0.338 0.3610.333 56.4 56.5 55.2 56.7 55.5 55.9 ' 55.7 55.7 60.7 53.6 MCH fnol MCHC itmol/1 RDW % 1.286 1.361 1.365 1.328 1.382 1.332 1.338 1.336 1.334 1.445 1.320 23.9 24.1 24.2 24.1 24.4 24.0 24.0 24.0 24.0 23.8 24.6 11.7 10.7 10.9 11.2 10.4 12.0 12.0 11.3 11.1 10.7 11.3 PLATELETS G/l 988 1068 1220 1027 1208 1229 1187 1069 1423 1033 1024 ANIMAL NUMBER 177 178 179 180 181 182 183 184 185 186 187 189 190 191 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 4.5 -- -- 7.4 11.8 1 1 .1 8.3 7.2 6.6 6.7 7.9 8.3 1 2 .1 0.055 -- -- 0.075 0.080 0.040 0.085 0.090 0.120 0.080 0.070 0.080 0.075 ___ 0.000 -- -- 0.005 0.005 0.005 0.005 0.000 0.010 0.010 0.000 0.010 0.005 BASO. 1 0.000 -- -- 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 MONO. 1 LYMPH. 1 -- 0.010 -- 0.015 0.020 0.025 0.025 0.010 0.030 0.025 0.020 0.025 0.015 0.935 -- ---- - 0.905 0.895 0.930 0.885 0.900 0.840 0.885 0.910 0.885 0.905 FT SEC 13.3 13.9 14.8 14.7 14.2 14.7 14.3 14.3 14.8 14.4 14.7 14.1 14.7 14.5 PTT SEC 13.6 12.9 16.7 14.6 16.8 17.7 20.0 20.5 15.0 16.2 15.8 16.4 16.0 18.1 1 97 NOTOX Project 242933 HAEMATOLOGY . AFTER 2 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN H E M - I N D - 13 30-MAR-99 ANIMAL NUMBER 9 10 11 HAEMATOLOGY PARAMETERS RBC HB HCT T/l ntnol/1 1/1 7.05 7.41 7.23 8.7 9.2 9.4 0.406 0.428 0.417 mcv fl 57.6 57.8 57.7 MCH fmol 1.234 1.242 1.300 MCHC mnol/1 21.4 21.5 22.5 RDW % 14.1 14.5 13.6 PLATELETS G/l 1293 1086 1152 ANIMAL NUMBER 9 10 11 HAEMATOLOGY PARAMETERS WBC SEG. ED. G/l 1 1 8.1 6.9 10.5 0.150 0.120 0.065 0.010 0.015 0.015 BASO. 1 0.000 0.000 0.000 MONO. 1 LYMPH. 1 0.030 0.025 0.025 0.810 0.840 . 0.895 PT SEC 14.6 14,3 15.0 PIT SEC 17.4 15.3 15.9 1 98 NOTOX Project 242933 HAEMATOLOGY . AFTER 2 WEEKS RECOVERY MALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN H E M - I N D - 14 3 0 - M A R - 99 ANIMAL NUMBER HAEMATOLOGY PARAMETERS RBC HB HOT T/X imol/1 1/1 25 . 6.79 26 7.06 27 7.28 8.8 8.9 8.9 0.395 0.407 0.409 mcv fl 58.2 57.6 56.2 M2H fmol 1.296 1.261 1.223 MCHC mmol/1 22.3 21.9 21.8 REW % 14.2 13.9 13.0 PLATELETS G/l 1198 1015 1030 ANIMAL NUMBER 25 26 27 HAEMATOLOGY PARAMETERS WBC SBS. EO. G/l 1 1 14.9 8.6 12.5 0.075 0.210 0.095 0.005 0.000 0.010 BASO. 1 0.000 0.000 0.000 MONO. 1 LYMPH. i 0.015 0.905 0.030 0.760 0.020 - 0.875 PT SEC 15.3 15.6 14.7 PTT SEC 15.0 16.1 14.8 1 99 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY HALES GROUP 3 (TELQMER ACRYLATE) BACK TO MAIN H EM-IND - 15 3O-MAR-9 9 ANIMAL NUMBER 41 42 43 HAEMATOLOGY PARAMETERS RBC HB HCT T/l mmol/1 1/1 6.83 6.80 7.28 8.7 8.3 9.2 0.396 0.386 0.421 mcv fl 58.0 56.8 57.8 MCH fmol 1.274 1.221 1.264 M2HC mnol/1 22.0 21.5 21.9 RDW 13.8 13.8 12.5 . PLATELETS G/l 1239 1323 995 ANIMAL NUMBER 41 42 43 HAEMATOLOGY PARAMETERS WBC SEE. BO. G/l 1 1 1 2 .1 13.9 10.2 0.090 0.040 0.135 0.010 0.000 0.005 BASO. 1 0.000 0.000 0.000 MONO. 1 0.025 0.020 0.030 LYMPH. 1 0.875 0.940 0.830 PT SEC 15.3 14.1 15.2 PIT SEC 17.1 15.8 15.2 200 BACK TO MAIN NOTOX Project 242933 - HAEMATOLOGY AFTER 2 WEEKS RECOVERY MALES GROUP 4 ( ) H E M - I N D - 16 3 0 - M A R - 99 * ANIMAL NUMBER 57 58 59 HAEMATOLOGY PARAMETERS RBC HB HOT T/l mrool/1 1/1 6.76 6.94 7.29 8.7 8.8 8.9 0.389 0.400 0.416 irvcv fl 57.5 57.6 57.1 MCH fmol 1.287 1.268 1.221 MCHC mmol/l 22.4 22.0 21.4 REW % 12.9 12.3 14.8 PLATELETS G/l ' 1099 1116 1024 ANIMAL NUMBER 57 58 59 HAEMATOLOGY PARAMETERS WBC SEG. BO. G/l 1 1 14.0 12.2 10.5 0.080 0.050 0.045 0.000 0.005 0.005 BASO. 1 0.000 0.000 0.000 MONO. 1 0.030 0.020 0.020 LYMPH. . FT 1 SEC 0.890 0.925 0.930 15.0 14.8 13.9 PTT SEC 16.2 15.3 13.6 201 BACK TO MAIN NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY MALES GROUP 5 (PFHS) HEM-IND - 17 3 0 - M A R - 99 ANIMAL NUMBER 73 74 75 HAEMATOLOGY PARAMETERS RBC T /l HB irm o l/1 HCT 1/1 6.86 6.84 7.21 8.5 8.5 8.7 0.388 0.395 0.394 m cv fl 56.6 57.7 54.6 MCH fm o l 1.239 1.243 1.207 MCHC m m o l/l 21.9 21.5 2 2 .1 RDM 14.0 15.0 14.2 PLATELETS G /l 1028 1071 1059 ANIMAL NUMBER 73 74 75 HAEMATOLOGY PARAMETERS NBC SBG. EO. G/l 1 1 11.1 6.4 7.9 0.200 0.075 0.125 0.015 0.010 0.010 BASO. 1 0.000 0.000 0.000 MONO. 1 0.025 0.010 0.020 LYMPH. 1 0.760 0.905 0.845 PT SEC 15.5 14.9 -- FTT SEC 15.3 15.0 -- 202 BACK TO MAIN NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY MALES GROUP 6 (PFOS) HEM-IND - 18 3 0 - M A R - 99 ANIMAL NUMBER 89 90 91 . HAEMATOLOGY PARAMETERS RBC HB HOT T/l mnol/1 1/1 6.76 7.15 7.13 8.9 8.9 8.9 0.401 0.412 0.411 mcv fl 59.3 57.6 57.6 MCH fmol 1.317 1.245 1.248 MCHC imvol/1 22.2 21.6 21.7 ROT % 14.0 12.9 12.5 PLATELETS G/l ' 1068 1026 1140 ANIMAL NUMBER 89 90 91 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 12.7 12.8 9.4 0.125 0.155 0.130 0.000 0.005 0.000 BASO. 1 0.000 0.000 0.000 MONO. 1 LYMPH. 1 0.020 0.020 0.015 ' 0.855 0.820 0.855 FT SEC . PIT SEC 15.6 ' 15.9 14.9 16.3 14.6 13.2 203 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN HEM-IND - 19 3 O-MAR-99 ANIMAL NUMBER 105 106 107 HAEMATOLOGY PARAMETERS RBC HB HCT T/l itmol/1 1/1 7.02 7.11 6.93 8.8 9.2 8.9 0.399 0.414 0.398 mcv fl 56.8 58.2 57.4 MCH fmol 1.254 1.294 1.284 MCHC irmol/1 22.1 22.2 22.4 REM % 15.8 12.9 14.7 PLATELETS G/l 1410 1083 1204 ANIMAL NUMBER 105 106 107 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 9.2 0.055 0.000 7.1 0.115 0.010 5.7 0.055 0.005 BASO. 1 0.000 0.000 0.000 MONO. 1 LYMPH. .1 0.025 0.015 0.010 0.920 0.860 0.930 PT SEC 16.2 15.5 16.3 PTT SBC 17.7 14.7 14.0 204 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN HEM-IND - 20 3 0 - M A R - 99 ANIMAL NUMBER 121 122 123 HAEMATOLOGY PARAMETERS RBC HB HOT T/l irmol/1 1/1 6.26 6.63 6.52 8.6 8.4 8.3 0.378 0.382 0.373 mcv fl 60.4 57.6 57.2 MCH fmol 1.374 1.267 1.273 MCHC nmol/1 22.8 22.0 22.3 REW % 14.8 16.4 14.4 PLATELETS G/l 1283 1024 1045 ANIMAL NUMBER 121 122 123 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 . 1 10.9 5.6 9.6 0.095 0.080 0.055 0.000 0.005 0.015 BASO. 1 0.000 0.000 0.000 MONO. 1 0.035 0.025 0.015 LYMPH. 1 0.870 0.890 0.915 PT SEC 14.6 14.9 15.3 FTT SEC 14.6 14.1 14.6 205 BACK TO MAIN NOTOX Project 242933 - HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 3 (TELOMER ACRYLATE) H E M - I N D - 21 3 0 - M A R - 99 A N IM AL NUMBER 137 138 139 haematology parameters RBC HB HCT T/l mmol/I 1/1 6.49 6.88 6.49 8.9 8.7 8.4 0.402 0.387 0.379 m cv fl 61.9 56.3 58.4 MCH finol . 1.371 1.265 1.294 MCHC mnol/1 22.1 22.5 22.2 REW PLATELETS % G/l 15.9 13.9 16.3 1053 1205 939 A N IM AL NUMBER HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 137 1 1 .0 138 6.0 139 4.8 0.060 0.050 0.085 . 0.005 0.005 0.000 baso : 1 0.000 0.000 0.000 MONO. 1 0.030 0.005 0.015 LYMPH. 1 PT SEC PTT SEC 0.905 0.940 0.900 ' 16.1 15.8 15.0 16.3 14.8 14.2 206 NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 4 ( ) BACK TO MAIN H E M - I N D - 22 3 0 - M A R - 99 ANIMAL NUMBER 153 154 155 HAEMATOLOGY PARAMETERS RBC HB HOT T/l irmol/1 1/1 6.59 7.27 6.27 8.8 9.4 8.7 - 0.397 0.408 0.384 mcv fl 60.2 56.1 61.2 MCH frnol 1.335 1.293 1.388 MCHC nmol/l 22.2 23.0 22.7. ROW 17.2 14.0 14.7 PLATELETS G/l 1060 1171 1102 ANIMAL NUMBER 153 154 155 HAEMATOLOGY PARAMETERS WBC G/l SEG. 1 EO. 1 7.9 0.075 0.005 6.9 0.050 0.015 5.3 0.115 0.010 BASO. 1 0.000 0.000 0.000 toro. 1 0.045 0.035 0.020 LYMPH. 1 0.875 0.900 0.855 PT PTT SEC SEC 14.8 ' 15.1 14.5 15.7 14.0 14.6 207 BACK TO MAIN NOTOX Project 242933 HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 5 (PFHS) H E M - I N D - 23 30-MAR-9 9 ANIMAL NUMBER 169 170 171 haem atology param eters RBC HB HCT T/l irmol/1 1/1 6.22 6.70 6.91 8.2 8.4 9.2 0.357 0.376 0.416 mcv fl 57.4 56.1 60.2 MCH . MCHC fmol ' irmol/1 1.318 1.254 1.331 23.0 22.3 22.1 RDM % 14.0 15.8 13.9 PLATELETS G/l 1010 828 723 ANIMAL NUMBER 169 170 171 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 5.9 0.085 0.005 7.7 0.035 0.020 6 .1 0.030 0.000 BASO. 1 0.000 0.000 0.000 MONO. 1 0.015 0.000 0.015 LYMPH. 1 0.895 0.945 0.955 PT SEC PIT SEC ... 14.7 14.9 16.6 14.8 208 BACK TO MAIN NOTOX Project 242933 HAEMATOLOGY - AFTER 2 WEEKS RECOVERY 6FEMALES GROUP (PFOS) H E M - I N D - 24 3 0-MAR-99 * ANIMAL NUMBER 185 186 187 HAEMATOLOGY PARAMETERS RBC HB HOT T/l rrmol/1 1/1 6.48 6.23 6.88 8.5 0.382 8.2 . 0.374 8.9 0.405 mcv fl 59.0 60.0 58.9 MCH fevol 1.312 1.316 1.294 MCHC nmol/1 22.3 21.9 22.0 ROT % 15.8 15.8 15.6 platelets G/l 1206 769 1127 ANIMAL NUMBER 185 186 187 HAEMATOLOGY PARAMETERS WBC SEG. BO. G/l 1 1 5.3 0.115 0.000 9.6 0.100 0.000 5.4 0.085 0.005 BASO. 1 0.000 0.000 0.000 MONO. 1 0.025 0.020 0.035 LYMPH. 1 0.860 0.880 0.875 PT PTT SEC SEC 15.4 ' 15.5 13.7 13.7 209 BACK TO MAIN NOTOX Project 242933 _ -..... HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL) H E M - I N D - 25 3O-MAR-9 9 * ANIMAL NUMBER 13 14 15 HAEMATOLOGY PARAMETERS RBC HB HOT T/l ntnol/1 1/1 7.52 7.75 6.79 8.9 9.8 8.7 0.391 0.424 0.373 mcv fl 52.0 54.7 54.9 MCH fmol 1.184 1.265 1.281 MCHC rmvol/1 22.8 23.1 23.3 ROW % 14.4 14.1 14.9 PLATELETS G/l 1062 981 1204 ANIMAL NUMBER 13 14 15 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 13.0 12.5 11.2 0.055 0.160 0.095 0.010 0.010 0.000 BASO. 1 0.000 0.000 0.000 MONO. 1 0.075 0.055 0.025 LYMPH. 1 0.860 0.775 0.880 PT SEC 12.7 -- 13.5 PIT SBC 16.1 -- 15.9 BACK TO MAIN NOTOX Project 242933 _ HAEMATOLOGY AFTER 4 WEEKS RECOVERY HALES GROUP 2 (TELOMER ALCOHOL) H E M - I N D - 26 3 0 - M A R - 99 * ANIMAL NUMBER 29 30 31 HAEMATOLOGY PARAMETERS RBC HB HOT T /l mmol/1 1/1 7.16 6.88 6.98 9.1 9.2 8.9 0.396 0.390 0.386 mcv fl 55.3 56.7 55.3 MCH fmol 1.271 1.337 1.275 MCHC irmol/1 23.0 23.6 23.1 REW % 12.7 12.6 13.5 PLATELETS G/l 917 1097 1062 ANIMAL NUMBER 29 30 31 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 10.7 10.5 15.8 0.120 0.065 0.100 0.010 0.000 0.000 BASO. 1 0.000 0.000 0.000 MONO. 1 0.075 0.040 0.050 LYMPH. 1 0.795 0.895 0.850 PT SEC 13.3 12.7 PTT SEC 14.6 15.7 21 1 BACK TO MAIN NOTOX Project 242933 - HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 3 (TELOMER ACRYLATE) HEM-XND - 27 3 0 - M A R - 99 * ANIMAL NUMBER 45 46 47 HAEMATOLOGY PARAMETERS RBC HB HOT T/l xnnol/1 1/1 6.84 6.70 7.01 8.9 8.9 9.4 0.372 0.386 0.395 mcv fl 54.4 57.6 56.3 MCH fool 1.301 1.328 1.341 MCHC ircnol/1 23.9 23.1 23.8 REW % 13.9 13.8 13.3 PLATELETS G/l 1244 960 1024 ANIMAL NUMBER 45 46 47 HAEMATOLOGY PARAMETERS NBC SEG. EO. G/l 1 1 15.6 8.6 8.2 0.085 0.125 0.060 0.005 0.015 0.010 BASO. 1 0.000 0.000 0.000 MONO. 1 0.025 0.085 0.030 LYMPH. 1 0.885 0.775 0.900 PT SEC 13.3 13.6 13.2 PTT SEC 16.7 16.6 17.0 21 2 BACK TO MAIN NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 4 ( ) HEM-IND - 28 3 0 - M A R - 99 ANIMAL NUMBER 61 62 63 HAEMATOLOGY PARAMETERS RBC KB HCT T/l nnol/1 1/1 6.84 7.18 7.01 9.2 9.5 9.0 0.379 0.408 0.388 xncv fi 55.4 56.8 55.3 MCH fznol 1.345 1.323 1.284 MCHC mnol/1 24.3 23.3 23.2 REW % 13.4 12.6 12.7 PLATELETS G/l 1232 1206 1100 ANIMAL NUMBER 61 62 ' 63 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 10.7 9.2 8.2 0.055 0.055 0.070 0.005 0.010 0.000 BASO. 1 0.000 0.000 0.000 MONO. 1 0.030 0.015 0.015 LYMPH. 1 0.910 0.920 0.915 PT SEC 13.0 14.6 PTT SEC 15.9 17.0 21 3 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 5 (PFHS) BACK TO MAIN H EM-IND - 29 3 0 - M A R - 99 ANIM AL NUMBER 77 78 79 HAEMATOLOGY PARAMETERS RBC HB HOT T/l nmol/1 1/1 6.82 6.87 6.97 8.9 8.7 9.4 0.384 0.381 0.406 m cv 1 56.3 55.5 58.2 MCH ftnol 1.305 1.266 1.349 M0HC nmol/1 23.2 22.8 23.2 REM t 13.2 13.8 13.8 PLATELETS G/l 1000 884 1013 ANIMAL NUMBER 77 78 79 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 9.5 0.060 0.005 7.1 0.065 0.010 6.5 0.065 0.005 BASO. 1 0.000 0.000 0.000 MONO. 1 0.020 0.035 0.020 LYMPH. 1 0.915 0.890 0.910 PT SEC 14.5 -- ' 13.8 PIT SEC 14.9 -- 15.5 214 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 6 (PFOS) BACK TO MAIN H EM-IND - 30 3 0 - M A R -9 9 ANIMAL NUMBER 93 94 95 HAEMATOLOGY PARAMETERS RBC HB HOT T/l ninol/1 1/1 7.14 7.20 6.63 8.9 9.2 8.8 0.383 0.406 0.378 mcv fl 53.6 56.4 57.0 MCH fmol 1.246 1.278 1.327 MCHC imvol/1 23.2 22.7 23.3 REW % 14.7 13.2 13.2 PLATELETS G/l 1083 1047 1101 A N IM A L NUMBER. 93 94 95 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 7.5 12.7 13.5 0.040 0.065 0.130 0.005 0.010 0.005 BASO. 1 0.000 0.000 0.000 MONO. 1 0.030 0.005 0.045 LYMPH. 1 0.925 0.920 0.820 PT . SEC PTT SEC 14.2 ' 13.9 13.8 13.2 16.9 13.7 21 5 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN HEM-IND - 31 3 0 - M A R - 99 ANIMAL NUMBER 109 110 111 HAEMATOLOGY PARAMETERS RBC HB HOT T/l mmol/l 1/1 6.76 7.09 6.76 9.2 9.4 9.2 0.380 0.423 0.389 mcv fl 56.2 59.7 57.5 MCH fmol 1.361 1.326 1.361 MCHC rrmol/1 24.2 22.2 23.7 ROW % 12.2 13.7 12.5 PLATELETS G/l 911 992 1013 ANIMAL NUMBER 109 110 111 HAEMATOLOGY PARAMETERS WBC SEG. BO. G/l 1 1 5.9 0.020 0.000 6.7 0.080 0.005 5.5 0.060 0.020 BASO. 1 MONO. 1 0.000 0.000 0.000 . 0.020 0.015 0.010 LYMPH. 1 0.960 0.900 0.910 PT PTT SEC SBC 13.0 12.0 11.8 14.6 16.9 15.6 21 6 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS RECOVERY FEMALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN HEM-IND - 32 3 0 - M A R - 99 AN IM AL NUMBER 125 126 127 HAEMATOLOGY PARAMETERS RBC HB HOT T/l nrnol/1 1/1 6.95 6.85 6.77 9.4 9.5 9.2 0.400 0.403 0.404 m cv fl 57.6 58.8 59.7 MCH fmol 1.353 1.387 1.359 MCHC mtnol/1 23.5 23.6 22.8 ROW % 13.1 14.0 13.4 platelets G /l 1088 882 969 ANIMAL NUMBER 125 126 127 HAEMATOLOGY PARAMETERS me SBG. EO. G/l 1 1 8.7 0.055 0.005 9.9 0.075 0.005 7.8 0.080 0.000 BASO. 1 0.000 0.000 0.000 MONO. 1 0.035 0.025 0.020 LYMPH. 1 0.905 0.895 0.900 PT PTT SEC SEC 11.9 12.2 ' 12.5 14.1 14.7 15.0 21 7 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS RECOVERY I*1k M A T Rfi GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN H E M - I N D - 33 3 0 - M A R - 99 ANIMAL NUMBER 141 142 143 HAEMATOLOGY PARAMETERS RBC HB HOT T/l rrmol/1 1/1 6.87 7.01 6.48 8.9 9.2 8.8 0.381 0.398 0.388 racv fl 55.5 56.8 59.9 MCH fmol 1.295 1.312 1.358 tHC irmol/1 23.4 23.1 22.7 ROW 13.3 13.1 13.4 platelets G/l 1093 841 961 ANIMAL NUMBER 141 142 143 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 10.7 8.9 3.5 0.025 0.055 0.035 0.010 0.010 0.010 BASO. 1 0.000 0.000 0.000 MONO. 1 0.035 0.010 0.010 LYMPH. 1 0.930 0.925 0.945 PT SEC 12.2 11.9 11.5 PIT SEC 14.6 16.7 15.9 21 8 BACK TO MAIN NOTOX Project 242933 HAEMATOLOGY ` AFTER 4 WEEKS RECOVERY FEMALES GROUP 4 ( ) HEM-IND - 34 3 0 - M A R - 99 AN IM AL NUMBER 157 158 159 HAEMATOLOGY PARAMETERS RBC HB HOT T/l nmol/1 1/1 7.25 6.88 6.85 9.2 8.9 9.1 0.402 0.386 0.391 mcv fl 55.4 56.1 57.1 MCH fmol - 1.269 1.294 1.328 MCHC nrool/1 22.9 23.1 23.3 RDW 12.9 12.5 12.3 PLATELETS G/l 1028 1045 969 HAEMATOLOGY PARAMETERS ANIMAL WBC NUMBER G/l SEG. 1 DO. 1 157 6.3 158 6.5 159 5.8 0.025 0.095 0.110 0.020 0.020 0.020 BASO. 1 0.000 0.000 0.000 MONO. 1 0.030 0.030 0.030 LYMPH. 1 0.925 0.855 0.840 PT SEC 12.5 11.5 12.6 PTT SEC 14.6 14.6 15.7 21 9 NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS RECOVERY FEMALES ROUP 5 (PFHS) BACK TO MAIN HEM-IND - 35 3 0 - M A R - 99 ANIMAL NUMBER 173 174 175 HAEMATOLOGY' PARAMETERS RBC HB HCT T/l innol/1 1/1 7.08 6.73 6.59 9.0 9.2 8.6 0.388 0.411 0.368 ircv fl 54.8 61.1 55.8 MCH fmol 1.271 1.367 1.305 MCHC mmol/1 23.2 22.4 23.4 REW % 13.7 1 2 .1 14.1 PLATELETS G/l 939 874 1120 ANIMAL NUMBER 173 174 175 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 6.9 0.095 0.010 4.5 0.065 0.005 9.1 0.065 0.005 BASO. 1 0.000 0.000 0.000 MONO. 1 0.040 0.050 0.015 LYMPH. 1 0.855 0.880 0.915 PT PIT SEC SEC 11.8 12.0 11.4 15.2 15.5 15.1 220 BACK TO MAIN NOTOX Project 242933 HAEMATOLOGY AFTER 4 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) HEM-IND - 36 3 0 - M A R - 99 ANIMAL NUMBER 189 190 191 haem atology param eters RBC HB HCT T/l irmol/1 1/1 6.49 6.73 6.45 8.7 9.2 8.1 0.370 0.413 0.355 m cv fl 57.0 61.4 55.0 MCH frool 1.341 1.367 1.256 MCHC ninol/1 23.5 22.3 22.8 REM % 11.9 13.3 13.5 PLATELETS G/l 1062 906 1013 ANIMAL NUMBER 189 190 191 HAEMATOLOGY PARAMETERS WBC SEG. EO. G/l 1 1 11.3 7.4 7.4 0.085 0.065 0.035 0.000 0.015 0.000 BASO. 1 0.000 0.000 0.000 MONO. .1 0.015 0.025 0.010 LYMPH. 1 0.900 0.895 0.955 PT SEC 12.0 12.7 12.0 PIT SEC 13.6 15.5 15.5 221 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN CHE-IND - 1 3 0 -M AR -99 ANIMAL NUMBER 1 2 3 4. 5 6 7 8 9 10 11 13 14 15 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHQLEST.T. TRIGL. mmol/1 ranol/1 0.88 0.69 0.79 0.60 0.66 0.51 0.67 0.50 0.58 0.70 0.56 0.54 0.53 0.80 3.21 2.55 2.34 3.28 3.30 2.77 2.76 2.16 2.42 2.33 3.13 2.92 2.86 3.10 3 1 2 1 3 3 3 2 3 3 2 2 2 3 1.58 1.94 2.63 2.36 1.47 2.14 2.07 1.70 2.47 2.53 1.53 2.04 1.85 1.64 , 0.75 0.80 0.63 1.27 0.54 0.83 1.12 0.59 0.53 0.88 0.61 1.05 0.52 1.07 CREATININE GLUCOSE UREA umol/1 mnol/1 mnol/1 35 6.63 6.5 29 5.67 5.9 37 6.01 8.8 31 5.65 5.8 32 7.14 6.5 29 7.50 5.9 29 6.31 5.5 28 5.56 6.5 38 6.70 8.1 38 6.04 8.6 33 6.93 7.7 38 6.14 7.5 30 4.94 5.9 39 7.31 6.9 ANIMAL NUMBER 1 2 3 4 5 6 7 8 9 10 11 13 14 15 PROTEIN T. ALBUMIN GLOBULIN A/G RATIO ALP g/1 g/1 g/1 ukat/1 54.4 56.6 57.5 57.8 59.9 59.1 62.5 57.5 55.2 50.8 53.5 55.9 57.6 56.6 29.0 29.6 30.4 30.4 30.4 28.9 30.2 28.5 28.5 27.7 30.0 29.2 28.6 29.2 25.4 27.0 27.1 27.4 29.5 30.2 32.3 29.0 26.7 23.1 23.5 26.7 29.0 27.4 1.1 1.1 1.1 1.1 1.0 1.0 0.9 1.0 1.1 1.2 1.3 1.1 1.0 1.1 12.54 7.29 6.03 7.45 8.56 6.65 6.99 8.54 6.11 10.02 7.26 7.58 7.29 6.26 INORG PHOS SODIUM mnol/1 mnol/1 2.76 3.22 2.79 2.63 2.86 2.71 2.85 3.04 2.73 2.70 2.78 2.80 3.00 2.78 143.5 143.8 143.1 143.1 144.8 142.3 143.3 142.8 141.6 143.1 143.3 141.3 139.8 140.6 POTASSIUM mmol/1 4.70 5.32 4.50 4.72 4.50 4.72 4.70 4.69 5.10 4.70 5.07 4.67 5.56 4.96 ANIMAL NUMBER CALCIUM mnol/1 CHLORIDE mnol/1 1 2.46 104 2 2.68 101 3 2.53 101 4 2.55 102 5 2.63 98 6 2.58 97 7 2.64 99 8 2.48 100 9 2.47 100 10 2.43 100 11 2.47 99 13 2.49 102 14 2.58 95 15 2.49 97 222 NOTOX Project 242933 - CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN CHE-IND - 2 3 0 - M A R - 99 * ANIMAL NUMBER 17 18 19 20 21 22 23 24 25 26 27 29 30 31 ALAT(GFT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. rrtnol/1 nmol/1 0.56 0.47 0.71 0.83 0.52 0.62 0.70 0.65 0.56 0.48 0.64 0.51 0.60 0.66 1.85 2.26 2.48 3.02 2.20 2.63 2.63 2.38 2.46 2.64 2.96 2.68 3.14 3.41 2 2 2 2 2 2 2 2 2 3 3 3 2 2 1.37 1.67 1.34 0.99 1.0 1 1.11 0.86 1.02 2.58 1.14 1.31 1.48 1.13 0.92 0.87 1.23 1.20 0.30 0.37 0.80 0.47 0.39 0.54 0.43 0.56 0.56 0.60 0.49 CREATININE GLUCOSE umol/1 nmol/1 urea' nmol/1 29 31 31 34 26 29 31 30 32 30 34 29 29 24 6.37 6.47 7.16 5.68 8.71 7.19 6.58 6.85 6.17 4.50 6.68 6.33 5.58 6.49 6.2 6.5 7.4 8.1 6.7 6 .1 6 .1 8.3 6.7 8.3 8.3 5.5 5.9 7.2 . ANIMAL NUMBER 17 18 19 20 21 22 23 24 25 26 27 29 30 31 PROTEIN T. ALBUMIN g/i g/i GLOBULIN A/G RATIO ALP g/i ukat/1 53.0 53.8 54.3 54.9 54.2 53.0 52.6 52.6 53.1 53.1 50.5 58.0 51.9 49.1 28.0 29.3 31.5 30.8 27.3 28.8 27.6 29.5 27.8 28.2 27.7 29.3 29.2 27.4 25.0 24.5 22.8 24.1 26.9 24.2 25.0 23.1 25.3 24.9 22.8 28.7 22.7 21.7 1 . 1 6.46 1 .2 8.92 1.4 7.59 1.3 6.81 1.0 8.12 1 .2 7.04 1 . 1 6.03 1.3 6.84 1 .1 . 8.21 1 . 1 11.53 1 .2 7.94 1 .0 9.19 1.3 12.49 1.3 6.01 INORO PHDS SODIUM mnol/1 mrol/1 2.60 3.04 2.93 2.69 2.64 2.90 2.69 2.76 2.65 2.71 2.72 2.80 2.61 2.76 143.9 143.3 143.1 143.3 143.4 142.6 142.1 141.4 142.3 143.5 141.8 144.8 142.5 141.9 POTASSIUM nmol/1 4.25 4.65 4.40 4.55 5.07 4.64 4.49 5.14 4.60 4.34 4.78 4.69 4.24 4.51 ANIMAL NUMBER CALCIUM nmol/1 CHLORIDE mmol/1 17 18 19 20 21 22 23 24 25 26 27 ` 29 30 31 2.58 2.48 2.55 2.50 2.49 2.47 2.43 2.44 2.42 2.42 2.37 2.59 2.43 2.44 101 100 102 103 94 99 101 99 101 102 101 100 101 100 223 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN CHE-IND - 3 3 0 - M A R - 99 ANIMAL NUMBER 33 34 35 36 37 38 39 40 41 42 43 45 46 47 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. nmol/1 nmol/1 0.58 0.85 0.64 0.62 0.52 0.45 0.81 0.44 0.48 0.50 0.73 0.72 -- 0.57 3.74 2.81 2.72 2.46 2.00 2.31 2.85 1.87 2.46 2.27 2.62 2.98 2.48 2.52 2 2 1 2 2 2 3 5 2 2 .2 3 2 2 1.23 2.72 1.19 0.89 1.13 1.91 1.13 1.10 1.64 1.40 1.51 1.72 1.02 1.33 1.72 0.71 0.62 0.44 0.66 1.34 0.46 0.68 1.13 0.56 0.43 0.51 0.43 0.57 CREATININE GLUCOSE umol/1 nmol/1 UREA nmol/1 31 6.30 5.7 29 5.63 6.0 34 6.80 7.2 33 5.77 7.2 28 6.56 5.7 29 6.66 7.5 30 6.95 6.2 32 7.48 8.5 31 7.21 5.7 31 6.26 8.1 30 5.24 6.2 34 5.48 7.7 31 5.86 6.5 27 5.22 4.9 AN IM AL NUMBER PROTEIN T. ALBUMIN . g/1 g /1 GLOBULIN A/G RATIO ALP g/i ukat/1 33 57.8 33.4 24.4 1.4 34 58.1 30.0 28.1 1 . 1 35 51.9 29.6 22.3 1.3 36 52.6 29.5 23.1 1.3 37 51.9 30.2 21.7 1.4 38 56.0 29.9 26.1 1 . 1 39 54.2 28.4 25.8 1 . 1 40 55.2 29.0 26.2 1 . 1 41 55.4 29.2 26.2 1 . 1 42 50.9 26.9 24.0 1 . 1 43 54.0 29.5 24.5 1 .2 45 52.7 29.2 23.5 1 .2 46 47.6 25.8 21.8 1.2 47 56.3 29.4 26.9 1 . 1 8.10 7.91 8.31 8.85 7.99 6.99 5.23 8.66 7.93 6.91 6.98 6.06 9.35 8.53 INORG PHDS SODIUM nmol/1 nmol/1 2.88 2.95 2.80 3.10 3.04 2.69 2.90 3.22 2.92 2.79 2.55 2.90 3.01 2.61 143.7 143.5 143.2 144.5 143.1 144.0 142.6 143.7 143.6 143.0 142.4 141.7 143.0 142.6 POTASSIUM nmol/1 4.81 3.92 4.81 4.32 4.95 4.85 4.66 5.14 5.30 4.69 4.58 4.81 5.13 4.09 . ANIMAL NUMBER 33 34 35 36 37 38 39 40 41 42 43 45 46 47 CALCIUM nrool/1 CHLORIDE nmol/1 2.57 2.53 2.38 2.62 2.48 2.55 2.48 2.57 2.54 2.44 2.47 2.50 2.44 2.46 101 100 103 102 102 100 100 102 104 104 102 101 103 101 224 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS . MALES GROUP 4 ( ) BACK TO MAIN CHE-IND - 4 30-MAR-99 ANIMAL NUMBER 49 50 51 52 53 54 55 56 57 58 59 61 62 63 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 iikat/1 umol/1 0.68 0.47 0.63 0.52 0.69 0.53 0.73 0.62 0.67 0.49 -- 0.45 0.38 0.52 2.49 2.41 2.09 2.30 2.85 2.16 2.43 2.19 2.48 2.58 2.52 2.28 1.67 . . 1.85 2 2 2 3 2 2 2 3 3 2 3 2 2 2 CHOLEST.T. TRIGL. imiol/1 mmol/l 1.62 1.73 1.75 1.76 1.65 1.39 1.33 2.56 2.37 2.23 1.54 1.15 1.98 1.51 0.56 0.58 1.22 0.85 0.51 0.50 0.73 0.84 0.66 0.75 0.60 0.45 0.50 0.40 CREATININE GLUCOSE umol/1 mnsl/l UREA mmol/l 35 34 29 31 29 37 34 32 42 32 35 38 40 . 31 7.70 6.58 8.07 7.05 5.86 7.41 7.04 7.39 7.38 5.75 6.29 6.57 4.62 4.58 7.8 6.7 . 6.4 6.8 5.7 5.6 7.1 6.9 8.6 6.8 6.2 6.6 9.0 6.2 ANIMAL NUMBER 49 50 51 52 53 54 55 56 57 58 59 61 62 63 PROTEIN T. ALBUMIN g/i g/x GLOBULIN A/G RATIO ALP g/x ukat/1 57.3 51.9 57.0 56.7 54.2 52.8 53.5 53.2 51.0 56.4 53.2 53.3 51.8 50.7 . 31.0 30.2 31.0 32.5 28.8 28.9 30.4 28.6 29.3 31.1 28.6 30.5 28.3 ' 28.2 26.3 21.7 26.0 24.2 25.4 23.9 23.1 24.6 21.7 25.3 . 24.6 22.8 23.5 22.5 1.2 1.4 1.2 1.3 1.1 1.2 1.3 1.2 1.4 1.2 1.2 1.3 1.2 1.3 8.01 7.08 8.24 10.97 7.10 9.03 8.06 8.42 8.47 7.39 6.63 6.76 7.03 8.81 INORG PHDS SODIUM mnol/1 mmol/l 2.56 2.84 2.67 2.59 2.83 2.72 2.54 2.72 3.19 2.40 2.58 2.76 3.15 2.45 143.8 143.1 141.3 142.6 143.2 143.4 142.4 143.0 142.5 142.7 143.3 143.0 143.8 144.3 POTASSIUM irmol/l 4.67 4.81 4.46 4.51 4.70 4.60 4.31 4.65 4.50 4.21 4.15 4.33 4.85 4.29 ANIMAL NUMBER CALCIUM mmol/l CHLORIDE irmol/1 49 2.45 101 50 2.43 104 51 2.52 100 52 2.55 98 53 2.48 102 54 2.41 102 55 2.45 102 56 2.36 101 57 2.54 103 58 2.57 101 59 2.37 99 61 2.47 95 62 2.56 104 63 2.45 102 225 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 5 (PFHS) BACK TO MAIN CHE-IND - 5 3 0 - M A R - 99 ANIMAL NUMBER 65 66 67 68 69 70 71 72 73 74 75 77 78 79 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 ianol/1 CHDLEST.T. TRIGL. irmol/1 rnmol/1 0.56 0.68 0.59 0.63 0.50 0.57 0.60 0.66 0.61 0.65 0.59 0.56 1.53 0.64 1.95 2.21 2.08 2.30 2.69 2.44 2.73 2.57 2.42 2.43 2.01 2.27 2.74 2.60 2 1 2 1 2 2 2 2 2 3 2 2 2 2 1.22 1.20 1.12 0.91 0.87 0.83 0.83 1.15 0.78 1.19 1.23 . 0.92 0.70 0.68 0.83 0.46 0.59 0.37 0.29 0.30 0.42 0.51 0.33 0.51 0.40 0.36 0.23 0.37 CREATININE GLUCOSE umol/1 mnol/1 UREA mmol/1 32 9.60 7.7 31 7.63 7.0 30 6.33 7.9 32 7.08 7.5 37 7.01 8.4 32 8.35 6.2 36 6.43 5.9 34 7.18 9.0 34 5.22 6.9 36 6.65 8.2 35 7.43 7.3 42 4.36 8.3 50 4.03 11.8 32 6.75 6.0 ANIMAL NUMBER 65 66 67 68 69 70 71 72 73 74 75 77 78 79 PROTEIN T. ALBUMIN g/1 g/1 GLOBULIN A/G RATTO ALP g/1 ukat/1 56.0 55.2 51.6 50.1 54.0 52.3 58.3 59.1 51.0 53.0 55.3 54.0 50.4 51.1 28.2 31.1 31.2 29.9 30.1 29.4 32.9 32.5 30.4 29.7 31.5 28.6 29.8 29.7 27.8 24.1 20.4 20.2 23.9 22.9 25.4 26.6 20.6 23.3 23.8 25.4 20.6 21.4 1.0 1.3 1.5 1.5 1.3 1.3 1.3 1.2 1.5 1.3 1.3 1.1 1.4 1.4 7.03 10.00 8.63 8.60 9.07 10.97 10.10 7.64 8.94 9.14 11.09 9.33 7.62 12.50 INORG PHQS SODIUM imnol/ 1 irmol/1 3.16 2.75 3.05 2.65 3.00 3.05 3.74 2.85 3.15 3.23 2.88 2.49 3.11 3.12 143.1 143.1 143.9 143.1 144.3 142.9 144.6 142.2 144.5 143.0 143.4 142.4 142.1 143.7 POTASSIUM nttiol/ 1 4.44 4.04 4.56 4.23 4.48 4.62 5.64 4.35 4.17 5.41 4.39 4.24 4.30 4.78 ANIMAL NUMBER 65 66 67 68 69 70 71 72 73 74 75 77 78 79 CALCIUM CHLORIDE irmol/1 mnol/1 2.66 2.45 2.56 2.40 2.54 2.51 2.64 2.53 2.54 2.56 2.44 2.40 2.49 2.48 100 101 103 101 105 102 105 104 104 102 98 103 103 106 226 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 6 (PFOS) BACK TO MAIN CHE-IND - 6 30-MAR-99 ANIMAL NUMBER 81 82 83 84 85 86 87 88 89 . 90 91 93 94 95 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. nmol/1 irmol/1 0.60 0.58 0.49 0.67 0.49 0.62 0.60 0.54 0.62 0.66 0.56 0.62 0.65 0.61 1.88 2.80 1.70 2.05 1.67 2.88 2.06 2.61 2.78 2.28 3.05 2.40 2.34 2.68 3* 1 2 2 3 3 2 3 3 3 3 2 2 2 1.99 1.09 0.81 1 .1 1 1.77 0.88 1.13 0.96 1.16 1.49 1.19 0.40 0.88 0.91 0.47 0.91 0.34 0.37 0.45 0.52 0.48 0.48 0.43 0.41 0.79 0.27 0.39 0.42 CREATININE GLUCOSE umol/1 mraol/1 UREA nmol/1 30 5.84 6.5 28 7.19 6.5 38 8.85 8.8 30 5.70 7.1 29 4.92 7.0 30 8.51 6 .1 37 6.72 10.7 30 4.50 8.1 33 6.45 7.9 29 6.00 7.4 37 5.13 9.0 30 6.20 7.7 28 6.92 6.9 32 6.35 6.9 ANIMAL NUMBER 81 82 83 84 85 86 87 88 89 90 91 93 94 95 PROTEIN T. ALBUMIN g /1 g/i GLOBULIN A/G RATIO ALP 3/1 ukat/1 53.0 54.9 54.9 55.9 53.1 54.7 57.5 51.6 53.0 53.7 56.4 49.7 53.6 55.0 31.2 30.3 31.4 29.5 30.1 29.2 29.4 28.2 28.5 28.4 27.7 28.0 28.1 29.7 21.8 1.4 24.6 1.2 23.5 1.3 26.4 1.1 23.0 1.3 25.5 1.1 28.1 1.0 23.4 ' 1 .2 24.5 1.2 25.3 1 .1 28.7 1.0 21.7 1.3 25.5 1 .1 25.3 1.2 5.41 8.58 9.87 4.56 5.55 8.46 7.00 7.24 7.98 6.12 8.68 8.14 7.23 6.78 INORG PHOS SODIUM umol/1 nmol/1 POTASSIUM nmol/1 2.60 2.95 3.11 2.95 2.91 2.80 3.07 2.83 3.01 3.37 3.16 2.67 2.94 3.03 143.4 143.9 143.1 142.7 144.6 143.3 143.3 143.2 143.2 142.0 141.8 141.9 142.5 141.6 . 4.39 4.99 5.27 5.00 3.98 4.55 4.39 4.67 4.46 4.75 4.59 4.72 5.15 4.34 ANIMAL NUMBER 81 82 83 84 85 86 87 88 89 90 91 93 94 95 CALCIUM CHLORIDE nrool/1 nmol/1 2.59 2.47 2.58 2.52 2.58 2.66 2.57 2.44 2.50 2.57 2.52 2.35 2.53 2.45 101 100 102 106 105 101 106 102 104 106 100 103 106 99 227 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS FEMALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN CHE-IND - 7 3 0 - M A R - 99 ANIMAL NUMBER 97 98 99 100 101 102 103 104 105 106 107 109 110 111 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 unvol/1 CHDLEST.T. TRIGL. mnol/1 mnol/1 0.69 0.39 0.44 0.69 0.61 0.53 0.47 0.52 0.65 0.40 0.46 0.33 0.64 0.46 2.74 1.94 2.47 3.09 2.94 2.97 2.75 2.70 2.39 2.35 2.86 2.26 2.93 2.22 4 3 2 2 4 3 3 2 2 3 2 3 2 3 2.74 1.68 2.44 1.81 1.97 1.87 1.75 2.44 1.68 2.86 1.92 2.45 2.47 2.81 0.81 0.33 0.71 0.40 0.80 0.51 0.44 0.62 0.48 0.88 0.37 0.68 0.69 0.74 CREATININE GLUCOSE unol/1 mnol/1 UREA mnol/1 39 37 35 . 40 42 41 38 46 38 39 44 ' 42 40 40 5.58 5.92 5.98 6.07 5.96 5.69 5.65 6.20 5.66 7.58 6.41 5.67 6.42 5.95 1 0 .1 6 .1 6.2 6.4 11.0 11.6 7.1 8.6 9.4 8.8 8.3 7.9 6.7 8.6 ANIMAL NUMBER PROTEIN T. ALBUMIN g/1 g/1 GLOBULIN A/G RATIO ALP g/1 ukat/1 97 63.1 98 67.9 99 . 61.3 100 67.4 101 67.1 102 61.5 103 70.1 104 70.9 105 67.3 106 65.5 107 64.8 109 60.8 n o 69.8 1 1 1 64.7 32.0 35.1 32.2 34.1 34.4 33.4 36.4 34.9 34.0 34.6 32.6 32.1 36.1 34.2 31.1 32.8 29.1 33.3 32.7 28.1 33.7 36.0 33.3 30.9 32.2 28.7 33.7 30.5 1.0 1.1 1.1 1.0 1.1 1.2 1 .1 1.0 1.0 1.1 1.0 1 .1 1.1 1 .1 3.33 4.34 5.17 5.53 4.09 4.41 3.91 5.50 4.21 3.87 4.50 4.79 4.35 4.39 INORO PHOS SODIUM mnol/1 mnol/1 POTASSIUM mnol/1 2.87 2.50 2.67. 2.61 2.68 2.93 2.27 2.52 2.87 2.68 2.50 2.12 2.37 2.49 142.4 144.1 146.1 139.8 143.7 142.1 141.6 141.7 141.0 141.4 142.0 141.4 142.7 141.1 4.85 3.41 3.96 4.59 4.03 4.78 4.08 4.18 4.13 4.09 3.77 3.91 3.87 4.21 ANIMAL NUMBER 97 98 99 100 101 102 103 104 105 106 107 109 110 111 CALCIUM mnol/1 CHLORIDE mnol/1 2.72 2.60 2.59 2.47 2.66 2.52 2.59 2.58 2.59 2.66 2.45 2.37 2.52 2.58 103 101 100 98 93 98 98 96 93 94 96 97 96 96 228 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS FEMALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN CHE-IND - 8 30-MAR-9 9 ANIMAL NUMBER 113 114 115 116 117 118 119 120 121 122 123 125 126 127 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 0.60 0.48 0.56 0.43 0.39 0.47 0.66 0.35 0.51 0.38 0.39 0.37 0.45 0.47 1.59 2.06 2.28 2.22 1.98 3.45 3.15 2.12 2.34 2.91 2.15 2.76 2.40 1.75 3 3 3 3 2 3 3 2 2 2 2 2 2 2 CHOLEST.T. TRIGL. mmol/1 nriol/1 2.41 2.66 2.22 2.56 1.70 2.14 2.86 2.14 2.24 2.15 1.46 2.57 1.85 2.69 0.47 0.52 1.07 0.48 0.47 0.60 0.69 0.46 0.52 0.60 0.42 0.63 0.31 0.69 CREATININE GLUCOSE umol/1 mtiol/1 UREA imtol/1 33 5.30 7.4 34 5.86 7.1 36 5.63 6.9 44 6.82 10 .1 35 6.08 7.2 42 6.00 11.7 40 5.66 9.1 36 5.90 8.6 41 7.40 9.5 39 5.79 7.4 36 5.70 6.3 37 5.76 8.5 39 5.38 8.6 44 5.29 8.7 ANIMAL NUMBER 113 114 115 116 117 118 119 120 121 122 123 125 126 127 PROTEIN T . ALBUMIN g/i g/i GLOBULIN g/1 A/G RATIO ALP u k a t/ 1 6 6 .6 6 6 .1 6 8.2 65.6 61.3 67.0 67.9 63.6 59.6 6 8 .8 64.6 63.3 63.2 61.3 34.2 30.8 34.1 34.0 32.5 35.0 33.7 33.6 32.5 37.0 34.6 34.2 33.9 32.9 32.4 35.3 34.1 31.6 28.8 32.0 34.2 30.0 27.1 31.8 30.0 29.1 29.3 28.4 1 .1 0.9 1.0 1 .1 1 .1 1 .1 1.0 1 .1 1 .2 1 .2 1 .2 1 .2 1 .2 1 .2 2.57 4.21 3.54 3.67 4.13 3.19 5.23 4.08 5.43 5.30 3.19 2.78 4.25 5.05 INORG PHOS SODIUM n tn o l/1 m m ol/1 2.57 2.69 2.43 2.63 2.28 2.70 2.69 2.71 2.60 2.39 2.48 2.57 2.41 2 .2 0 141.5 142.5 143.3 143.1 143.0 142.2 140.9 142.5 143.2 143.5 142.0 142.4 142.7 143.7 POTASSIUM B inol/1 4.20 4.43 4.08 3.91 3.79 4.79 4.55 4.14 4.44 4.19 3.79 4.04 4.10 3.49 ANIMAL NUMBER 113 114 115 116 117 118 119 120 121 122 123 125 126 127 CALCIUM mnol/1 CHLORIDE ramol/1 2.66 2.60 2.67 2.63 2.50 2.57 2.63 2.60 2.57 2.65 2.57 2.65 2.54 2.51 102 99 102 99 100 101 97 96 96 98 96 98 97 99 229 NOTOX Project 242933 _ CLINICAL BIOCHEMISTRY AFTER 4 WEEKS FEMALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN CHE-IND - 9 30-MAR-99 * ANIMAL NUMBER 129 130 131 132 133 134 135 136 137 138 139 141 142 143 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 unl/l CHOLEST.T. TRIGL. irmol/1 runol/1 0.30 0.57 0.47 0.49 0.84 0.76 0.66 0.60 0.43 0.44 0.47 0.55 0.58 0.51 1.66 2.18 2.08 1.97 2.32 2.15 2.60 2.45 1.99 2.82 1.73 2.47 2.12 2.82 2 3 2 2 3 3 3 3 2 2 3 3 3 3 1.74 1.83 1.76 2.50 2.88 2.53 2.17 1.68 1.41 2.03 1.99 2.14 2.18 2.39 0.51 0.49 0.67 0.56 0.74 0.81 0.80 0.62 0.41 0.60 0.53 0.38 0.40 0.59 CREATININE GLUCOSE umol/1 umol/1 UREA nnvol/1 32 5.27 5.5 37 5.23 8.4 34 6.67 7.0 37 5.78 9.4 37 6.32 10.4 38 5.83 9.7 44 5.62 9.5 38 6.25 8.5 45 5.83 11.4 40 6.89 8.4 40 6.20 7.3 41 6.08 11.3 38 5.98 7.0 43 7.96 9.6 ANIMAL NUMBER 129 130 131 132 ' 133 134 135 136 137 138 139 141 142 143 PROTEIN T. ALBUMIN g/1 g/1 GLOBULIN A/G RATIO ALP g/1 ukat/1 64.4 66.2 68.0 62.0 69.5 62.8 65.6 66.7 60.2 65.8 64.2 66.9 66.4 66.8 32.3 34.0 35.3 33.5 34.8 32.0 33.5 34.5 31.2 35.0 36.2 33.8 35.5 36.4 32.1 32.2 32.7 28.5 34.7 30.8 32.1 32.2 29.0 30.8 28.0 - 33.1 30.9 30.4 1.0 1 .1 1.1 1.2 1.0 1.0 . 1.0 1.1 1 .1 1 .1 1.3 1.0 1.1 1.2 3.01 4.48 3.02 5.49 3.20 5.05 5.59 3.32 5.91 5.72 3.94 6.27 3.43 4.90 INORG PHOS SODIUM irmol/1 umol/1 2.43 2.58 2.40 2.73 2.41 2.72 2.69 2.38 2.29 2.45 2.14 2.64 2.55 2.10 143.8 143.2 143.1 142.7 142.8 140.9 140.9 143.4 142.6 144.3 143.0 142.0 143.1 140.8 POTASSIUM smol/1 3.81 4.28 3.64 3.97 3.89 4.31 4.41 4.04 4.44 3.63 4.01 4.59 4.02 4.56 ANIMAL NUMBER 129 130 131 132 133 134 135 136 137 138 139 141 142 143 CALCIUM mmol/1 CHLORIDE runol/1 2.60 2.60 2.54 2.57 2.72 2.55 2.64 2.61 2.52 2.60 2.65 2.63 2.64 2.54 99 99 100 99 98 96 96 96 100 98 96 102 95 100 230 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS FEMALES GROUP 4 ( ) BACK TO MAIN C H E - I N D - 10 3 0 - M A R - 99 ANIMAL NUMBER 145 146 147 148 149 150 151 152 153 154 155 157 158 159 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. nmol/1 nmol/1 0.41 0.26 0.49 0.57 0.44 0.61 0.56 0.55 0.49 0.47 0.54 0.49 0.68 0.74 2.21 2.15 2.27 1.80 1.74 2.67 2.34 2.21 2.67 2.18 2.64 2.99 1.97 2.31 2 3 3 2 3 5 4 4 2 2 2 3 3 3 1.73 2.25 2.61 2.38 2.20 2.70 2.80 2.08 ' 2.04 2.55 2.14 2.83 2.05 2.09 0.65 0.53 0.46 0.56 0.73 0.47 0.87 0.44 0.56 0.56 0.42 0.45 0.48 0.62 CREATININE GLUCOSE umol/1 nmol/1 UREA nmol/1 37 5.98 6.7 42 6.02 8.2 41 5.37 8.5 34 5.79 7.1 36 6.92 9.8 42 5.40 10.6 43 6.00 9.9 45 5.98 10.6 43 5.78 8.9 37 6.69 5.5 43 6.21 10.9 44 5.68 9.2 40 5.39 8.4 46 6.74 11.3 ANIMAL NUMBER PROTEIN T. ALBUMIN g/1 g/1 GLOBULIN A/G RATIO ALP g/1 ukat/1 145 67.8 146 67.5 147 . 64.8 148 61.7 149 67.7 150 67.2 151 65.3 152 70.3 153 70.7 154 60.9 155 65.0 157 67.6 158 67.0 159 63.3 35.0 33.0 30.1 32.0 34.5 34.1 34.7 37.6 38.3 32.9 32.4 32.7 33.6 32.8 32.8 34.5 34.7 29.7 33.2 33.1 30.6 32.7 32.4 28.0 32.6 34.9 33.4 30.5 1.1 1.0 0.9 1.1 1.0 1.0 1.1 1 .1 1.2 1.2 1.0 0.9 1.0 1 .1 3.53 5.95 4.82 4.31 3.45 3.68 3.07 3.66 3.72 5.74 4.54 4.17 5.22 4.36 INORG PHOS SODIUM nmol/1 nmol/1 2.18 2.34 2.30 2.46 2.60 2.61 2.90 2.29 2.25 2.33 2.47 2.28 2.50 2.30 143.7 143.0 141.9 140.4 142.7 142.0 141.6 143.4 141.9 141.9 143.2 140.6 141.5 140.8 POTASSIUM nmol/1 3.82 4.06 4.45 3.91 4.47 4.31 4.28 3.82 4.30 4.24 4.46 4.37 3.93 4.30 ANIMAL NUMBER 145 146 147 148 149 150 151 152 153 154 155 157 158 159 CALCIUM nmol/1 CHLORIDE nmol/1 2.57 2.63 2.52 2.58 2.64 2.63 2.66 2.64 2.60 2.57 2.55 2.58 2.68 2.50 100 103 99 98 95 98 . 98 97 101 99 102 100 100 99 231 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS FEMALES GROUP 5 (PFHS) BACK TO MAIN CHE-IND - 11 3 0 - M A R - 99 ANIMAL NUMBER 161 162 163 164 165 166 167 168 169 170 171 173 174 175 ALAT(GPT) ASAT(GOT) .BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. itmol/1 umol/1 0.52 0.50 0.78 0.53 0.57 0.49 0.57 0.62 0.65 0.59 0.60 0.40 0.62 0.53 1.77 3.54 2.18 1.61 2.01 3.77 2.27 2.86 2.49 1.75 1.61 2.79 2.88 2.73 3 3 2 3 3 2 3 2 2 2 2 2 2 3 1.93 2.16 1.64 1.80 2.23 1.76 1.46 1.94 1.59 2.06 2.16 1.32 1.66 1.68 1.00 0.52 0.48 0.32 0.55 0.45 0.49 0.55 0.59 0.44 0.44 0.38 0.33 0.53 CREATININE GLUCOSE umol/1 irmol/1 UREA itmol/1 38 6.34 7.7 40 . 5.01 7.2 38 4.93 9.6 35 6.21 7.9 35 5.64 7.8 37 5.33 7.6 39 6.02 9.6 45 5.96 9.1 44 5.77 8.5 40 5.92 8.2 42 7.44 7.8 44 6.18 8.6 44 6.23 9.2 42 7.30 9.9 ANIMAL NUMBER 161 162 163 164 165 166 167 168 169 170 171 173 174 175 PROTEIN T. ALBUMIN g/l g/l GLOBULIN A/G RATIO ALP g/l ukat/1 74.6 66.1 71.1 66.5 63.9 60.5 63.1 66.2 63.2 62.4 70.0 64.5 60.1 63.5 35.6 32.4 35.0 34.7 33.3 31.3 33.8 33.9 32.2 33.2 35.8 33.8 31.3 35.4 39.0 33.7 36.1 31.8 30.6 29.2 29.3 32.3 31.0 29.2 34.2 30.7 28.8 28.1 0.9 1.0 1.0 1 .1 1.1 1 .1 1.2 1.0 1.0 1.1 1.0 1 .1 1 .1 1.3 3.89 6.82 4.63 3.95 3.02 4.31 3.49 4.11 5.07 3.03 3.41 2.49 5.06 3.54 INORG PHOS SODIUM irmol/1 itmol/1 2.16 2.28 2.42 2.32 2.81 2.79 2.64 2.56 2.39 2.21 2.35 2.47 2.36 2.22 141.3 141.8 141.5 142.2 141.9 143.1 141.9 142.3 140.8 142.7 142.8 141.0 141.1 141.0 POTASSIUM umol/1 3.90 3.86 3.90 4.06 4.19 4.49 3.89 4.34 4.14 3.70 3,91 4.53 4.47 3.79 ANIMAL NUMBER CALCIUM umol/1 CHLORIDE irmol/1 161 2.70 104 162 2.57 101 163 2.62 98 164 2.53 102 165 2.61 99 166 2.54 99 167 2.53 103 168 2.55 101 169 2.55 104 170 2.52 105 171 2.64 98 173 2.52 104 174 2.50 102 175 2.51 103 232 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER. 4 WEEKS FEMALES GROUP 6 (PFOS) BACK TO MAIN C H E - I N D - 12 3 0-MAR-9 9 ANIMAL NUMBER 177 178 179 180 181 182 183 184 185 186 187 189 190 191 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. nrool/1 nmol/1 0.56 0.42 0.72 0.42 0.38 0.63 0.78 0.48 0.51 0.67 0.45 0.57 0.61 1.67 2.04 2.09 2.36 2.48 2.45 1.85 2.32 1.52 2.59 2.95 2.59 2.39 2.77 2.01 2 2 3 4 3 3 4 3 3 3 2 2 2 3 2.56 1.46 2.29 2.14 1.71 2.48 2.19 1.97 1.96 2.00 1.46 . 2.47 2.06 1.83 0.60 0.30 0.38 0.52 0.58 0.82 0.63 0.63 0.58 0.38 0.33 0.65 0.77 0.39 CREATININE GLUCOSE umol/1 irmol/1 UREA nrool/1 41 5.31 11.3 35 5.90 8.6 38 4.83 7.9 47 5.02 . 12.6 36 5.85 9.1 41 6.81 1 1 .1 43 4.80 9.8 49 5.38 11.6 44 5.12 9.3 39 5.93 9.8 41 6.39 8.2 42 7.96 10.3 37 7.71 8.2 45 6.64 14.4 ANIMAL NUMBER 177 178 179 180 181 182 183 184 185 186 187 189 190 191 PROTEIN T. ALBUMIN g/i g/i GLOBULIN A/G RATIO ALP 3/1 ukat/1 65.0 66.6 71.5 65.3 67.5 67.5 70.6 68.8 68.8 63.9 63.1 61.2 64.5 70.3 35.1 35.7 33.3 32.6 35.9 34.7 37.6 35.8 35.3 34.0 33.8 32.2 35.0 38.1 29.9 30.9 38.2 32.7 31.6 32.8 33.0 33.0 33.5 29.9 29.3 29.0 29.5 32.2 1.2 1.2 0.9 1.0 1.1 1 .1 1 .1 1.1 1 .1 1.1 1.2 1 .1 1.2 1.2 2.84 3.47 3.85 3.07 3.12 4.80 5.14 4.60 4.12 3.71 7.14 4.32 5.50 4.50 INORG PHDS SODIUM imol/1 nmol/1 2.55 2.15 2.47 2.69 2.92 2.62 2.64 3.01 2.77 2.74 2.37 2.57 2.36 2.37 143.7 143.4 143.3 142.2 142.9 142.2 142.0 143.4 141.8 144.3 141.9 142.5 141.7 140.0 POTASSIUM nmol/1 4.03 3.80 3.77 4.14 4.09 4.03 3.82 3.77 4.87 4.05 3.84 4.41 4.27 3.95 ANIMAL NUMBER CALCIUM nmol/1 CHLORIDE imol/1 177 2.58 178 2.54 179 2.59 180 2.56 181 2.59 182 2.73 183 2.72 184 2.62 185 . 2.63 186 2.49 187 2.57 189 2.59 190 2.53 191 2.63 101 102 99 99 102 103 99 104 99 103 104 102 102 99 233 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN C M E - X N D - 13 3 0 - M A R - 99 ANIMAL NUMBER 9 10 11 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. nnvol/l xnonol/1 0.82 1.07 0.70 2.50 2.46 3.43 3 3 3 2.35 2 .11 1.39 0.49 0.46 0.63 CREATININE GLUCOSE umol/1 mmol/1 UREA itmol/ 1 43 5.79 7.2 35 5.21 7.9 28 5.95 5.3 ANIMAL NUMBER 9 10 11 PROTEIN T. ALBUMIN g/i g/i 57.6 54.9 55.1 28.4 28.4 30.1 GLOBULIN A/G RATIO ALP g / i ukat/1 29.2 26.5 25.0 1.0 1.1 1.2 4.77 8.85 6.16 INORG FHOS SODIUM mmol/1 mnol/1 POTASSIUM innol/1 2.87 2.76 2.80 144.9 144.9 143.0 4.53 4.01 4.85 ANIMAL NUMBER 9 10 11 CALCIUM nxnol/1 CHLORIDE innol/1 2.52 2.44 2.48 103 102 103 234 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN CHE-IND - 14 3 0 - M AR-99 ANIMAL NUMBER 25 26 27 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 .ukat/1 urnol/1 0.71 0.58 0.56 2.42 3.16 2.52 3 3 2 CHOLEST.T. TRIGL. ranol/1 rtmol/1 2.42 1.07 1.53 0.67 0.39 0.77 CREATININE GLUCOSE umol/1 imol/1 UREA irmol/1 36 7.39 6.8 33 4.33 7.4 32 6.96 7.7 ANIMAL NUMBER 25 26 27 PROTEIN T. ALBUMIN g/i g/i GLOBULIN A/G RATIO ALP g/i ukat/1 52.0 50.3 52.2 28.6 27.7 28.0 23.4 22.6 24.2 1.2 1.2 1.2 7.04 7.89 5.86 INORG PHDS SODIUM ninol/1 irmol/1 2.94 2.76 2.63 142.1 145.9 142.3 POTASSIUM ltmol/1 4.59 4.26 4.69 ANIMAL NUMBER 25 26 27 CALCIUM m nol/1 CHLORIDE um ol/1 2.50 2.39 2.44 100 105 102 235 NOTOX Project 242933 -.... CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN C H E - I N D - 15 3O-MAR-9 9 * ANIMAL NUMBER 41 42 43 ALAT(GPT) ASAT(GOT) B IL I T . ukat/1 ukat/1 umol/1 0.61 0.55 0.78 2.89 2.95 2.89 3 3 2 CHOLEST.T. TRIGL. mnol/1 mnol/1 1.61 1.57 1.54 1.23 0.89 0.54 CREATININE GLUCOSE umol/1 mmol/1 UREA mnol/1 31 7.79 6.2 34 6.81 7.5 33 5.94 7.1 ANIMAL NUMBER PROTEAN T. ALBUMIN g/i g/ 1 GLOBULIN A/G RATIO ALP g/i ukat/1 41 57.5 42 56.8 43 . 58.6 29.7 29.2 29.6 27.8 27.6 29.0 1.1 1.1 1.0 7.13 4.72 5.60 INORO PHOS SODIUM mnol/1 mmol/1 POTASSIUM mnol/1 2.98 2.73 2.52 142.6 142.2 141.1 4.97 5.07 4.82 ANIMAL NUMBER CALCIUM mnol/1 CHLORIDE mmol/1 41 2.51 42 2.46 43 . 2.47 99 102 99 236 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 4 ( ) BACK TO MAIN CHE-IND - 1 6 3 0 -MAR-99 ANIMAL NUMBER 57 58 59 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. mnol/1 mnol/1 0.87 0.66 1.0 1 2.49 3.54 ' 2.56 3 3 2 2.30 2.03 1.45 0.82 0.62 0.67 CREATURINE GLUCOSE umol/1 tnnol/1 UREA mnol/1 40 8.32 6.7 37 8.45 7.4 40 8.23 6.4 ANIMAI* NUMBER 57 58 59 PROTEIN T. ALBUKEN g/i g/i 52.3 57.3 51.3 27.9 29.1 26.5 GLGBULIN A/G RATIO ALP g /i ukat/1 24.4 28.2 24.8 1 .1 1.0 . 1 .1 6.34 6.70 4.78 INORG FHOS SODIUM mnol/1 umol/1 2.73 2.68 2.52 140.3 141.2 141.7 POTASSIUM mnol/1 4.35 4.64 4.25 ANIMAL NUMBER 57 58 59 CALCIUM umol/1 CHLORIDE mnol/1 2.57 2.56 2.37 99 101 101 237 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTETR 2 WEEKS RECOVERY MALES GROUP 5 (PFHS) BACK TO MAIN CHE-IND - 17 3 0 -MAR-99 ANIMAL NUMBER 73 74 75 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHDLEST.T. TRIGL. nmol/l nraol/1 0.71 0.96 0.75 2.82 2.49 1.99 2 2 2 1.29 1.63 1 .2 1 0.58 0.62 0.75 CREATININE GLUCOSE umol/l nmol/l UREA. nmol/l 39 7.25 6.6 42 6.96 7.6 35 7.21 6.0 ANIMAL NUMBER 73 74 75 PROTEIN T. ALBUMIN g/1 g/1 GLOBULIN A/G RATIO ALP g/i ukat/1 53.9 55.1 55.1 28.4 28.2 30.0 25.5 26.9 25.1 1.1 1.0 1.2 5.85 6.89 7.43 INORG PHOS SODIUM nmol/l nmol/l 2.67 2.43 2.42 142.1 143.4 141.7 POTASSIUM nmol/l 4.71 4.57 4.41 ANIMAL NUMBER 73 74 75 CALCIUM n m ol/l CHLORIDE n m ol/l 2.44 2.49 2.42 101 104 100 238 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 6 (PFOS) BACK TO MAIN C H E - I N D - 18 30-MAR-9 9 ANIMAL NUMBER ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. mtiol/1 nmol/1 89 0.83 90 0.61 91 ' 0.49 2.48 1.63 1.66 2 3 3 1.22 1.48 1.12 0.51 0.40 0.60 CREATININE GLUCOSE umol/1 nmol/1 UREA nmol/1 34 7.21 7.3 34 6.29 6.7 49 5.17 8.1 ANIMAL tJMBER 89 90 91 PROTEIN T. ALBUMIN g/i g/l GLOBULIN A/G RATIO ALP 3/1 ukat/1 56.3 55.9 57.3 30.0 29.0 30.1 26.3 26.9 27.2 1 .1 1 .1 1 .1 6.36 4.22 6.26 INORG PHOS SODIUM nmol/1 nmol/1 POTASSIUM nmol/1 2.52 2.67 2.75 142.8 142.7 142.8 . 4.52 4.82 4.35 ANIMAL NUMBER 89 90 91 CALCIUM nmol/1 CHLORIDE mnol/1 2.53 2.65 2.53 101 102 3-05 239 NOTOX Project 242933 ____ CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL} BACK TO MAIN CHE-IND - 19 3 0 - M A R - 99 ANIMAL NUMBER 105 106 107 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. mtnol/1 mnol/1 0.52 0.43 0.46 2.50 1.96 3.12 3'. 3 2 1.95 2.59 1.93 0.56 0.97 0.55 CREATININE GLUCOSE umol/1 mnol/1 UREA mnol/1 37 6.72 6.7 38 9.25 6.2 41 6.06 7.6 ANIMAL NUMBER 105 106 107 PROTEIN T. ALBUMIN g/i g/i 65.2 65.3 60.4 32.4 34.7 29.4 GLOBULIN A/G RATIO ALP g/i ukat/1 32.8 30.6 31.0 1.0 1.1 0.9 3.43 3.21 3.26 INORG PHDS SODIUM mnol/1 mnol/1 POTASSIUM mnol/1 2.45 2.39 2.56 143.3 142.5 141.4 . 3.98 4.14 4.12 ANIMAL NUMBER 105 106 107 CALCIUM mnol/1 CHLORIDE rnicl/1 2.60 2.79 2.51 98 99 99 240 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 2 WEEKS RECOVERY FEMALES GROUP 2 (TELOMER ALCOHOL) BACK TO MAIN CHE-IND - 20 3 0 - M A R - 99 ANIMAL NUMBER ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 121 0.52 122 1.02 123 . 0.36 2.44 3.36 2.02 2 3 2 CHOLEST.T. TRIGL. mnol/1 mnol/1 2.14 2.20 1.42 0.43 0.40 0.46 CREATININE GLUCOSE umol/1 nrool/1 UREA mmol/1 40 7.26 6.3 41 6.31 8.9 38 5.98 6.7 ANIMAL NUMBER 121 122 123 PROTEIN T. ALBUMIN g/i g/i GLOBULIN A/G RATIO ALP g/i ukat/1 58.1 61.3 65.6 29.6 34.9 33.6 28.5 26.4 32.0 1.0 1.3 1.1 4.38 4.07 2.34 INORG PHDS SODIUM mnol/1 mnol/1 2.64 2.89 2.18 142.4' 144.2 141.7 POTASSIUM mnol/1 3.95 4.16 3.77 ANIMAL NUMBER 121 122 123 CALCIUM mnol/1 CHLORIDE ranol/1 2.54 2.65 2.59 98 100 99 241 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 2 WEEKS RECOVERY FEMALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN C H E - X N D - 21 3 0 - M A R - 99 ANIMAL NUMBER 137 138 139 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 0.58 0.51 0.37 2.27 2.35 1.69 2 2 2 CHDLEST.T. TRIGL. nrool/1 innol/1 1.27 1.97 1.97 0.39 0.87 0.57 CREATININE GLUCOSE umol/1 mmol/l UREA nmol/1 37 6.71 7.1 40 7.44 9.3 47 7.77 8 .1 ANIMAL NUMBER 137 138 139 PROTEIN T. ALBUMIN ff/1 g / i 60.7 62.9 59.7 29.5 32.5 32.9 GLOBULIN A/G RATIO ALP g / l ukat/1 31.2 30.4 26.8 0.9 1.1 1.2 5.56 4.02 3.49 INORG PROS SODIUM rnnol/1 sin o l/1 2.27 2.38 2.30 143.1 141.4 141.5 POTASSIUM mmol/1 3.76 4.54 3.86 ANIMAL NUMBER 137 138 139 CALCIUM imcl/1 CHLORIDE umol/1 2.46 2.61 2.63 98 103 96 242 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY FEMALES GROUP 4 ( ) _ ' 3 BACK TO MAIN C H E - I N D - 22 0-MAR-99 ANIMAL NUMBER 153 154 155 ALAT(GPT) ASAT(GOT) BILI T. ' CHQLEST.T. TRIGL. ukat/1 ukat/1 umol/1 irmol/1 nmol/1 0.48 0.63 0.60 2.35 2.32 2.93 3 2 3 2.17 2.72 2.34 1.03 0.53 0.63 CREATININE GLUCOSE umol/1 nmol/1 UREA. nmol/1 43 7.47 9.5 39 8.41 8.9 53 6.34 11.0 ANIMAL NUMBER 153 154 155 PROTEIN T. ALBUMIN g/i 9/1 GLOBULIN A/G RATIO ALP g/i ukat/1 67.8 61.8 66.1 35.7 32.4 33.4 32.1 29.4 32.7 1 .1 1 .1 1.0 3.09 4.81 3.65 INORG PHDS SODIUM nmol/1 nmol/1 POTASSIUM nmol/1 2.70 2.23 2.71 142.7 139.7 141.4 3.90 4.61 4.50 ANIMAL NUMBER 153 154 155 CALCIUM nmol/1 CHLORIDE nmol/1 . 2.69 2.64 2.70 100 98 97 .' 243 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY FEMALES GROUP 5 (PFHS) _ " BACK TO MAIN C H E - I N D - 23 30-MAR-99 * ANIMAL NUMBER 169 170 171 ALAT(GPT) ABAT (GOT) BILI T. ukat/1 ukat/1 umol/1 CHDLEST.T. TRIGL. nmol/l irmol/1 0.48 0.43 0.65 1.83 1.56 1.58 3 3 .3 1.66 2.42 2.24 0.50 0.35 0.34 CREATININE GLUCOSE uxnol/l nmol/l UREA irmol/1 46 7.25 7.7 39 6.01 7.2 47 9.38 7.9 ANIMAL NUMBER 169 170 171 PROTEIN T. ALBUMIN 3/1 g / i GLOBULIN A/G RATIO ALP g / i ukat/1 65.5 62.8 71.0 30.7 30.9 32.5 34.8 31.9 38.5 0.9 1.0 0.8 4.62 2.30 2.90 INORO PROS SODIUM nmol/l nmol/l 2.37 2.19 2.70 141.1 141.1 142.5 POTASSIUM nmol/l 3.98 3.65 4.08 ANIMAL NUMBER 169 170 171 CALCIUM nmol/l CHLORIDE nmol/l 2.58 2.62 2.84 100 99 95 244 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY 6FEMALES GROUP (PFOS) BACK TO MAIN CHE-IND - 24 3 0-MAR-99 ANIMAI, NUMBER 185 186 187 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 0.37 0.51 0.48 2.22 2.15 1.89 2 3 2 CHQLEST.T. TRIGL. nmol/1 mmol/1 2.04 2.37 1.64 0.40 0.25 0.29 CREATININE GLUCOSE umol/1 nmol/l UREA umol/1 -43 8.03 8.2 41 7.67 9.0 42 7.37 6.7 ANIMAL NUMBER 185 186 187 PROTEIN T. ALBUMIN ff/1 g / i 66.6 57.4 57.9 33.9 31.6 32.0 GLOBULIN A/G RATIO ALP g n ukat/1 32.7 25.8 25.9 1 .0 1.2 1.2 3.66 3.25 6.04 INORG PHOS SODIUM nmol/1 nmol/1 2.40 2.21 2.27 141.9 143.4 142.2 POTASSIUM nmol/1 3.97 4.27 4.08 ANIMAL NUMBER 185 186 187 CALCIUM nmol/1 CHLORIDE nmol/1 2.65 2.56 2.69 99 103 99 245 NOTOX Project 242933 _ CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL) BACK TO MAIN CHE-IND - 25 30-MAR-99 * ANIMAL NUMBER 13 14 15 ALAT(GET) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 0.78 0.54 0.44 2.88 2.39 2.69 4 3 3 CHOLEST.T. TRIGL. nrool/1 ltmol/1 2.07 2.01 1.73 1.17 0.39 0.71 CREATININE GLUCOSE umol/1 nmol/1 UREA itmol/ 1 37 8.42 6 .1 35 7.63 6 .1 37 7.97 6.7 ANIMAL NUMBER 13 14 15 PROTEIN T. ALBUMIN g/i g/i 62.1 62.2 62.2 29.3 28.2 28.4 GLOBULIN A/G RATIO ALP g / i ukat/1 32.8 34.0 33.8 0.9 0.8 0.8 4.61 4.79 3.58 INORG PHOS SODIUM nmol/1 nmol/1 2.91 3.17 2.99 140.8 142.5 141.6 POTASSIUM nmol/1 5.15 5.56 5.28 ANIMAL NUMBER 13 14 15 CALCIUM nmol/1 CHLORIDE nmol/1 2.56 2.60 . 2.58 104 101 102 246 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY MALES GROUP 2 (TELOMER ALCOHOL) --" BACK TO MAIN CHE-IND - 26 30-MAR-99 . ANIMAL NUMBER ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHQLEST.T. TRIGL. ranol/1 mnol/1 29 0.70 2.66 2 30 0.61 2.49 2 31 0.74 2.17 3 1.39 1.22 1.14 0.57 0.55 0.61 CREATININE GLUCOSE umol/1 mnol/1 UREA mnol/1 29 5.87 6.5 33 8.32 6.3 36 8.16 6.2 ANIMAL NUMBER 29 30 31 PROTEIN T. ALBUMIN g/1 g/1 GLOBULIN A/G RATIO ALP g/1 ukat/1 INORG PHOS SODIUM mnol/1 nmol/1 57.8 54.2 55.6 26.7 27.5 27.7 31.1 26.7 27.9 0.9 1.0 1.0 6.15 8.13 4.31 2.44 2.51 2.96 143.4 141.4 143.9 POTASSIUM nnvol/1 4.53 4.50 4.75 ANIMAL NUMBER 29 30 31 CALCIUM mnol/1 CHLORIDE mmol/1 2.44 2.46 2.55 106 103 101 247 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY MALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN CHE-IND - 27 3 0 - M A R - 99 ANIMAL NUMBER 45 46 47 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 0.62 0.57 0.60 2.00 2.33 1.86 3 2 3 CHOLEST.T. TRIGL. ittnol/1 mmol/l 1.93 1.16 1.51 0.42 0.53 0.64 CREATININE GLUCOSE umol/1 . mmol/l UREA mmol/l 32 7.91 7.4 39 7.38 6.7 31 5.88 5.6 ANIMAL NUMBER 45 46 47 PROTEIN T. ALBUMIN g/l g/i 58.4 51.6 56.2 27.7 25.4 27.9 GLOBULIN A/G RATIO ALP ' g/l ukat/1 30.7 26.2 28.3 0.9 1.0 1.0 3.83 6.75 5.27 INORG PHDS SODIUM mmol/l mmol/l 2.67 2.86 2.38 141.4 143.8 142.3 POTASSIUM rnnol/ 1 4.86 4.81 4.26 ANIMAL NUMBER CALCIUM mmol/l CHLORIDE ncnol/l 45 2.53 102 46 2.47 105 47 2.44 104 24 8 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY MALES GROUP 4 ( ) BACK TO MAIN C H E - I N D - 28 3 0 - M A R - 99 ANIMAL NUMBER 61 62 63 ALAT(GET) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 0.67 0.38 0.69 2.96 2.16 2.05 3 2 3 CHQLEST.T. TRIGL. nmol/1 nmol/1 1.37 2.05 1.56 0.55 0.59 0.55 CREATININE GLUCOSE umol/1 nmol/1 UREA nmol/1 39 5.58 6.9 37 6.68 8.2 39 6 .11 7.3 ANIMAL NUMBER 61 62 ' 63 PROTEIN T. ALBUMIN g/i g/i GLOBULIN A/G RATIO ALP g/1 ukat/1 59.5 54.9 56.6 29.0 27.7 26.5 30.5 27.2 30.1 1.0 1.0 0.9 4.68 4.66 6.94 INORG PHDS SODIUM POTASSIUM umol/1 umol/1 . nmol/1 2.46 2.64 2.28 . 141.5 141.8 142.7 4.61 4.62 4.41 ANIMAL NUMBER _> 61 62 63 CALCIUM nmol/1 CHLORIDE nmol/1 2.49 2.45 2.44 103 . 104 105 249 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY MALES GROUP 5 (PFHS) BACK TO MAIN CKE-IND - 29 30-MAR-99 ANIMAL NUMBER 77 78 79 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. nmol/1 irtnol/1 0.60 0.94 0.71 2.61 2.21 2.38 3 2 2 1.22 1.34 1.16 0.57 0.26 0.24 CREATININE GLUCOSE umol/1 nxnol/ 1 UREA nmol/1 47 5.95 8.1 47 4.77 10.4 36 6.00 6.5 ANIMAL NUMBER 77 78 79 PROTEIN T. ALBUMIN g/i g/i GLOBULIN A/G RATIO ALP g / i ukat/1 57.9 58.1 56.0 27.0 28.0 27.0 30.9 30.1 29.0 0.9 0.9 0.9 5.79 4.17 8.04 INORO PHOS SODIUM nmol/1 nmol/1 2.55 2.55 2.86 143.5 142.9 144.9 POTASSIUM nmol/1 4.40 4.26 5.04 ANIMAL NUMBER 77 78 79 CALCIUM nmol/1 CHLORIDE mnol/1 2.48 2.49 2.51 104 103 103 250 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY HALES GROUP 6 (PFOS) BACK TO MAIN C H E - I N D - 30 3 0 - M A R - 99 ANIMAL NUMBER 93 94 95 ALAT(GPT) ASAT(GOT) BILI T. ufcat/1 ukat/l urool/1 CHDLEST.T. TRIGL. mmol/1 umol/1 0.56 0.77 0.58 1.99 1.73 1.89 2 2 2 0.92 1.47 1.62 0.35 0.58 0.43 CREATININE GLUCOSE umol/1 nrool/1 UREA mmol/1 29 6.01 5.8 31 7.23 5.6 36 6.85 6.5 ANIMAL NUMBER 93 94 95 PROTEIN T. ALBUMIN 3/1 g / i 51.7 55.0 56.7 27.0 27.3 28.3 GLOBULIN A/G RATIO ALP g /1 ukat/l 24.7 27.7 28.4 1.1 1.0 1.0 4.89 4.78 4.52 INORG PHDS SODIUM mnol/1 mmol/1 2.58 2.40 2.79 142.8 142.7 ' 142.5 POTASSIUM mcnol/ 1 4.60 4.78 4.58 ANIMAL NUMBER CALCIUM ninol/1 CHLORIDE mmol/1 . 93 - 2.43 94 2.52 95 2.49 105 107 109 251 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL) _ BACK TO MAIN CHE-IND - 31 3 0 - M A R - 99 ANIMAL NUMBER 109 110 111 ALAT(GPT) ABAT(GOT) BILI T. ukat/1 ukat/1 umol/1 0.41 0.45 0.28 2.28 2.54 1.92 3 2 2 CHOLEST.T. TRIGL. mmol/1 mmol/1 2.25 2.31 2.94 0.60 0.78 0.52 CREATININE GLUCOSE umol/1 . mmol/1 UREA mmol/1 45 6.19 7.7 40 8.58 5.9 39 6.70 9.0 ANIMAL NUMBER 109 110 111 PROTEIN T. ALBUMIN g/i 0/1 61.4 69.4 63.8 30.1 33.2 30.5 GLOBULIN A/G RATIO ALP 0/1 ukat/1 31.3 36.2 33.3 1.0 0.9 0.9 3.41 2.49 2.96 INORG PHDS SODIUM mmol/1 . mmol/1 2.46 2.14 2.39 144.0 142.5 140.5 POTASSIUM mmol/1 3.84 4.01 4.50 ANIMAL CALCIUM NUMBER itmol/1 CHLORIDE mmol/1 109 2.47 103 110 2.55 101 1 1 1 2.55 96 . 252 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY FEMALES GROUP 2 (TELOMER ALCOHOL) -- BACK TO MAIN CHE-IND - 32 30-MAR-99 . ANIMAL NUMBER 125 126 127 AIiAT(GFT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. mnol/1 mnol/1 0.39 0.34 -- 2.66 2.26 2.01 2 2 3 2.68 2.01 2.55 1.00 0.47 0.76 CREATININE GLUCOSE umol/1 mmol/1 UREA mnol/1 39 6.83 6.7 40 6.54 6.8 48 5.91 9.0 ANIMAL NUMBER 125 126 127 PROTEIN T. ALBUMIN g/i g/i 67.0 62.2 63.6 32.2 30.3 32.4 GLOBULIN A/G RATIO ALP g / i ukat/1 34.8 31.9 31.2 ' 0.9 0.9 1.0 1.73 3.18 4.19 INORO PHDS SODIUM mnol/1 mnol/1 2.26 2.35 2 .11 141.4 142.2 141.3 POTASSIUM mnol/1 4.25 4.36 3.82 ANIMAL NUMBER 125 126 CALCIUM mnol/1 CHLORIDE mnol/1 2.64 2.49 104 98 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY FEMALES GROUP 3 (TELOMER ACRYLATE) BACK TO MAIN C H E - I N D - 33 3 0 - M A X - 99 ANIMAL NUMBER 141 142 143 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHDLEST.T. TRIGL. nmol/1 nmol/1 0.43 0.36 0.59 1.74 1.54 2.40 2 3 3 2.18 2.39 2.32 0.43 0.50 0.49 CREATININE GLUCOSE umol/1 itmol/1 UREA nmol/1 40 6.64 8.3 41 6.73 6.9 45 7.32 8.8 ANIMAL NUMBER 141 142 143 PROTEIN T. ALBUMIN g /1 g/i 65.5 67.8 69.9 29.5 34.7 35.9 GLOBULIN A/G RATIO ALP g/i ukat/1 36.0 33.1 34.0 0.8 1.0 1 .1 3.86 2.08 3.40 INORG PHDS SODIUM nmol/1 zrmol/1 2.19 1.95 1.79 138.7 141.6 141.7 POTASSIUM nmol/1 5.43 3.66 4.34 ANIMAL NUMBER 141 142 143 CALCIUM imol/l CHLORIDE mnol/1 2.47 2.60 2.59 96 103 102 254 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY FEMALES GROUP 4 ( ) ~ BACK TO MAIN CHE-IND - 34 30-MAR-99 . ANIMAL NUMBER 157 158 159 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. itmol/1 itmol/1 0.43 0.98 0.47 2.75 2.30 2.31 2 2 2 2.99 2.52 2.02 0.41 0.40 0.72 CREATININE GLUCOSE umol/1 irmol/1 UREA mmol/1 43 5.89 8.2 40 8.08 6.9 48 5.05 9.9 ANIMAL NUMBER 157 158 159 PROTEIN T. ALBUMIN g/1 g/i GLOBULIN A/G RATIO ALP g/i ukat/1 64.8 68.9 62.2 30.3 34.5 32.0 36.9 29.4 ' 32.8 0.9 0.9 0.9 2.48 3.91 2.40 INORO PHDS SODIUM ranol/1 umol/1 2.20 1.93 2.52 141.0 142.1 140.9 POTASSIUM mmol/1 4.51 3.67 4.59 ANIMAL NUMBER 157 158 159 CALCIUM mmol/1 CHLORIDE mmol/1 2.60 2.57 2.53 100 96 101 2 55 NOTOX Project 242933 CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY FEMALES GROUP 5 (PFHS) BACK TO MAIN CHE-IND - 35 3 0 - M A R - 99 ANIMAL NUMBER 173 174 175 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. raiGL. nmol/l nmol/l 0.33 0.64 0.45 2.27 3.09 2.21 2 2 2 1.68 2.12 2.37 0.40 0.34 0.46 CREATININE GLUCOSE umol/1 nmol/l UREA nmol/l 38 6.77 6.8 39 6.50 6.9 39 7.31 7.3 ANIMAL NUMBER 173 174 175 PROTEIN T. ALBUMIN g/1 g/1 68.5 69.4 70.2 34.9 33.5 34.2 GLOBULIN A/G RATIO ALP g/i ukat/1 33.6 35.9 36.0 1.0 0.9 1.0 1.78 3.46 2.85 INORG PHDS SODIUM nmol/l nmol/l 2.15 2.08 2.24 139.9 140.5 142.6 POTASSIUM irmol/1 4.17 4.25 3.65 ANIMAL NUMBER 173 174 175 CALCIUM nmol/l CHLORIDE nmol/l 2.59 2.59 2.61 104 99 102 256 NOTOX Project 242933 CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) BACK TO MAIN CHE-IND - 3S 3 0-M A R -9 9 ANIMAL NUMBER 189 190 191 ALAT(GPT) ASAT(GOT) BILI T. ukat/1 ukat/1 umol/1 CHOLEST.T. TRIGL. mmol/1 nrool/1 0.52 0.49 0.50 1.96 1.94 1.86 2 2 3 2.38 1.99 2.01 0.39 0.74 0.37 CREATININE GLUCOSE unol/l nmol/1 UREA nmol/1 39 6.77 9.4 38 8.87 7.9 39 7.31 14.9 ANIMAL NUMBER 189 190 191 PROTEIN T. ALBUMIN 3/1 3/1 59.5 66.2 70.1 30.6 33.2 34.6 GLOBULIN A/G RATIO ALP 3/1 ukat/1 28.9 33.0 35.5 1 .1 1.0 1.0 3.56 3.19 3.40 INORG PHDS SODIUM nmol/1 nmol/1 2.56 2.18 2.07 142.7 141.0 139.5 POTASSIUM nmol/1 4.13 4.36 4.00 ANIMAL NUMBER 189 190 191 CALCIUM nmol/1 CHLORIDE nmol/1 2.63 2.61 2.67 100 108 100 2 57 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS ' BACK TO MAIN MAC-IND - 1 25-FEB-99 MACROSCOPIC FINDINGS MALES GROUP 1 (VEHICLE CONTROL) ANIMAL 1 NO FINDINGS NOTED ANIMAL 2 NO FINDINGS NOTED ANIMAL 3 NO FINDINGS NOTED ANIMAL 4 NO FINDINGS NOTED ANIMAL 5 NO FINDINGS NOTED ANIMAL 6 NO FINDINGS NOTED ANIMAL 7 NO FINDINGS NOTED ANIMAL 8 NO FINDINGS NOTED ANIMAL 9 SKIN............... CHEST REGION: ALOPECIA. ANIMAL 10 NO FINDINGS NOTED ANIMAL 11 NO FINDINGS NOTED * (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-N0V-93) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY. 04-NOV-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY. 18-N0V-98) (SCHEDULED NECROPSY, 1B-N0V-98) 25 8 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ '/PFHS/PFOS " ' BACK TO MAIN MAC-IND - 2. 25-FEB-99 MACROSCOPIC FINDINGS MALES GROUP 1 (VEHICLE CONTROL) ANIMAL 13 NO FINDINGS NOTED ANIMAL 14 NO FINOINGS NOTED ANIMAL 15 NO FINDINGS NOTED . . (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) 259 NOTOX Project 242933 '- TELOMER ALCOHOL/TELOMER ACRYLATE/ _ ,/PFHS/PFOS " BACK TO MAIN MAC-IND - 3 25-FEB-99 MACROSCOPIC FINDINGS MALES GROUP 2 (TELOMER ALCOHOL) . ANIMAL 17 NO FINDINGS NOTED ANIMAL 18 NO FINDINGS NOTED ANIMAL 19 ` NO FINDINGS NOTED ANIMAL 20 NO FINOINGS NOTED ANIMAL 21 NO FINOINGS NOTED ANIMAL 22 NO FINDINGS NOTED ANIMAL 23 NO FINDINGS NOTED ANIMAL 24 NO FINDINGS NOTED ANIMAL 25 . NO FINDINGS NOTED ANIMAL 26 NO FINDINGS NOTED ANIMAL 27 TESTES............. REDUCED IN SIZE, FLACCID. (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-93) (SCHEDULED NECROPSY, 04-N0V-9B) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) 260 NOTOX Project 242933 *- TELOMER ALCOHOL/TELOMER ACRYLATE/ _ /PFHS/PFOS " BACK TO MAIN MAC-IND - 4 25-FEB-99 MACROSCOPIC FINDINGS MALES GROUP 2 (TELOMER ALCOHOL) ANIMAL 29 NO FINDINGS NOTED ANIMAL 30 NO FINDINGS NOTED ANIMAL 31 NO FINDINGS NOTED (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) ' (SCHEDULED NECROPSY, 02-DEC-98) 261 NOTOX P r o je c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS BACK TO MAIN MAC-IND - 5 25-FEB-99 MACROSCOPIC FINDINGS MALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 33 NO FINDINGS NOTED ANIMAL 34 NO FINDINGS NOTED ANIMAL 35 NO FINDINGS NOTED ANIMAL 36 NO FINDINGS NOTED ANIMAL 37 NO FINDINGS NOTED ANIMAL 38 NO FINDINGS NOTED ANIMAL 39 NO FINDINGS NOTED ANIMAL 40 NO FINDINGS NOTED ANIMAL 41 NO FINDINGS NOTED ANIMAL 42 NO FINDINGS NOTED ANIMAL 43 NO FINDINGS NOTED . (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY. 04-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) 262 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS ` BACK TO MAIN MAC-IND - 6 2 5 - F E B -99 MACROSCOPIC FINDINGS MALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 45 NO FINDINGS NOTED ANIMAL 46 NO FINDINGS NOTED ANIMAL 47 NO FINDINGS NOTED {SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-93) (SCHEDULED NECROPSY, 02-0EC-93) 263 NOTOX Project 242933 -- TELOMER ALCOHOL/TELOMER ACRYLATE. _ JPFHS/PFOS ' BACK TO MAIN MAC-IND - 7 25-FEB-99 MACROSCOPIC FINDINGS MALES GROUP 4 ( ) ANIMAL 49 . NO FINDINGS NOTED ANIMAL SO NO FINDINGS NOTED ANIMAL 51 NO FINDINGS NOTED ANIMAL 52 NO FINDINGS NOTED ANIMAL 53 NO FINDINGS NOTED ANIMAL 54 NO FINDINGS NOTED ANIMAL 55 NO FINDINGS NOTED ANIMAL 56 NO FINDINGS NOTED . ANIMAL 57 NO FINDINGS NOTED ANIMAL 58 NO FINDINGS NOTED ANIMAL 59 , NO FINOINGS NOTED (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) . 264 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS " BACK TO MAIN MAC-IND - 8 25-FEB-99 MACROSCOPIC FINDINGS MALES GROUP4 ( ) ANIMAL 61 EYES........ RIGHT SIDE, LENS: REDUCED IN SIZE. ANIMAL 62 NO FINDINGS NOTED ANIMAL 63 NO FINDINGS NOTED (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) 265 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS MACROSCOPIC FINDINGS MALES GROUP 5 (PFHS) ANIMAL 65 NO FINDINGS NOTED ANIMAL 66 LUNGS.............. STOMACH............ TESTES............. EPIDIDYMIDES....... HEMORRHAGE. GLANDULAR MUCOSA: HEMORRHAGE. REDUCEO IN SIZE. REDUCED IN SIZE. ANIMAL 67 NO FINDINGS NOTED ANIMAL 68 NO FINDINGS NOTED ANIMAL 69 NO FINDINGS NOTED ANIMAL 70 NO FINDINGS NOTED ANIMAL 71 NO FINDINGS NOTED ANIMAL 72 LIVER.............. ENLARGED. ANIMAL 73 NO FINDINGS NOTED ANIMAL 74 TESTES............. REDUCED IN SIZE. BACK TO MAIN MAC-IND 9 2 5 - F E B -99 (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY. 03-N0V-98) (SCHEDULED NECROPSY, 03-NOV-9B) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY. 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) 266 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -_ /PFHS/PFOS MACROSCOPIC FINDINGS MALES GROUP 5 (PFHS) ANIMAL 75 NO FINDINGS NOTED ANIMAL 77 NO FINDINGS NOTED ANIMAL 78 NO FINDINGS NOTED ANIMAL 79 NO FINDINGS NOTED . BACK TO MAIN MAC-IND -10 25-FEB-99 (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) 267 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS ' BACK TO MAIN MAC-IND -11 25-FEB-99 MACROSCOPIC FINDINGS MALES GROUP 6 (PFOS) ANIMAL 81 . NO FINDINGS NOTED ANIMAL 82 NO FINDINGS NOTED ANIMAL 83 NO FINDINGS NOTED ANIMAL 84 NO FINDINGS NOTED ANIMAL 85 NO FINDINGS NOTED ANIMAL 86 NO FINDINGS NOTED ANIMAL 87 NO FINDINGS NOTED ANIMAL 88 NO FINDINGS NOTED ANIMAL 89 ' NO FINDINGS NOTED ANIMAL 90 LIVER.............. ACCENTUATED LOBULAR PATTERN. ANIMAL 91 NO FINDINGS NOTED . (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 04-NOV-9S) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-NOV-98) 268 NOTOX Project 242933 -- TELOMER ALCOHOL/TELOMER ACRYLATE/^ /PFHS/PFOS ' BACK TO MAIN MAC-IND -12 25-FEB-99 MACROSCOPIC FINDINGS MALES GROUP 6 (PFOS) ANIMAL 93 NO FINDINGS NOTED ANIMAL 94 NO FINDINGS NOTED ANIMAL 95 NO FINDINGS NOTED (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) 269 NOTOX Project 242933 -- TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS ` BACK TO MAIN MAC-IND -13 25-FEB-99 MACROSCOPIC FINDINGS FEMALES GROUP 1 (VEHICLE CONTROL) ANIMAL 97 . NO FINOINGS NOTED ANIMAL 98 OVARIES............ LEFT SIDE: WATERY CYST. ANIMAL 99 NO FINDINGS NOTED ANIMAL 100 NO FINDINGS NOTED ANIMAL 101 NO FINDINGS NOTED ANIMAL 102 NO FINDINGS NOTED ANIMAL 103 NO FINOINGS NOTED ANIMAL 104 NO FINDINGS NOTED ANIMAL 105 SKIN............... ABDOMINAL REGION: SCAB FORMATION. ANIMAL 106 NO FINDINGS NOTED ANIMAL 107 NO FINOINGS NOTED (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-N0V-9B) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY. 06-NOV-98) (SCHEDULED NECROPSY. 20-NOV-98) (SCHEDULED NECROPSY. 20-N0V-98) (SCHEDULED NECROPSY. 20-N0V-98) 270 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -./PFHS/PFOS ' BACK TO MAIN MAC-IND -14 2 5 - F E B -99 MACROSCOPIC FINDINGS FEMALES . GROUP 1 (VEHICLE CONTROL) ANIMAL 109 NO FINDINGS NOTED ANIMAL 110 NO FINDINGS NOTED ANIMAL 111 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY. 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-9 8 ) 271 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -VPFHS/PFOS * BACK TO MAIN MAC-IND -15 25-FEB-99 MACROSCOPIC FINDINGS FEMALES . GROUP 2 (TELOMER ALCOHOL) ANIMAL 113 NO FINDINGS NOTED ANIMAL 114 NO FINDINGS NOTED ANIMAL 115 NO FINDINGS NOTED ANIMAL 116 NO FINDINGS NOTED ANIMAL 117 KIDNEYS........................... RIGHT SIDE: PELVIC DILATION. ANIMAL 118 NO FINDINGS NOTED ANIMAL 119 NO FINDINGS NOTED ANIMAL 120 NO FINDINGS NOTED ANIMAL 121 NO FINDINGS NOTED ANIMAL 122 SKIN.................................. ALOPECIA. ANIMAL 123 NO FINDINGS NOTED * (SCHEDULED NECROPSY, 05-N0V-98) . (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 0 5 -NOV-9 8 ) (SCHEDULED NECROPSY, 0 5 -NOV-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY,' 06-NOV-98) (SCHEDULED NECROPSY, 0 6 -NOV-9 8 ) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY. 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 2 0 -NOV-98) 272 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -!PFHS/PFOS ' BACK TO MAIN MAC-IND -16 25-FEB-99 MACROSCOPIC FINDINGS FEMALES . GROUP 2 (TELOMER ALCOHOL) ANIMAL 125 NO FINDINGS NOTED ANIMAL 126 NO FINDINGS NOTED ANIMAL 127 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) . (SCHEDULED NECROPSY, 04-DEC-98) 273 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -_ 7 PFHS/PFOS MACROSCOPIC FINDINGS FEMALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 129 NO FINDINGS NOTED ANIMAL 130 NO FINDINGS NOTED ANIMAL 131 NO FINDINGS NOTED ANIMAL 132 NO FINDINGS NOTED ANIMAL 133 NO FINDINGS NOTED ANIMAL 134 NO FINDINGS NOTED ANIMAL 135 NO FINDINGS NOTED ANIMAL 136 NO FINDINGS NOTED ANIMAL 137 NO FINDINGS NOTED ANIMAL 138 NO FINDINGS NOTED ANIMAL 139 NO FINDINGS NOTED BACK TO MAIN MAU-1INU -1/ 2 5 - F E B -99 (OTHER, 05-N0V-98) (SCHEDULED NECROPSY. 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY. 06-N0V-98) (SCHEDULED NECROPSY, 06-NOV-9 8 ) (SCHEDULED NECROPSY. 06-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-NOV-98) 274 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS BACK TO MAIN MAC-IND -18 25-FEB-99 MACROSCOPIC FINDINGS FEMALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 141 NO FINDINGS NOTED ANIMAL 142 NO FINDINGS NOTED ANIMAL 143 NO FINDINGS NOTED * (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) 275 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS MACROSCOPIC FINDINGS FEMALES GROUP 4 ( ) ANIMAL 145 NO FINDINGS NOTED ANIMAL 146 NO FINDINGS NOTED ANIMAL 147 NO FINDINGS NOTED ANIMAL 148 NO FINDINGS NOTED ANIMAL 149 NO FINDINGS NOTED ANIMAL 150 NO FINDINGS NOTED ANIMAL 151 UTERUS............................. LEFT SIDE: CYST(S). ANIMAL 152 NO FINOINGS NOTED ANIMAL 153 NO FINDINGS NOTED ANIMAL 154 NO FINDINGS NOTED ANIMAL 155 NO FINDINGS NOTED BACK TO MAIN ` MAC-IND -19 25-FEB-99 '* (SCHEDULED NECROPSY, 05-N0V-98 ) (SCHEDULED NECROPSY, 05-NOV-9 8 ) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 06-NOV-9 8 ) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 0 6 -NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98) (SCHEDULED NECROPSY, 2 0 -NOV-98) (SCHEDULED NECROPSY, 20-N0V-98) 276 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -,, /PFHS/PFOS BACK TO MAIN MAC-IND -20 25-FEB-99 MACROSCOPIC FINDINGS FEMALES GROUP 4 ( ) ANIMAL 157 NO FINDINGS NOTED ANIMAL 158 NO FINDINGS NOTED ANIMAL 159 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) 277 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ .^ /PFHS/PFOS BACK TO MAIN MAC-IND -21 25-FEB-99 MACROSCOPIC FINDINGS FEMALES GROUP 5 (PFHS) ANIMAL 161 NO FINDINGS NOTED ANIMAL 162 NO FINDINGS NOTED ANIMAL 163 NO FINDINGS NOTED ANIMAL 164 NO FINDINGS NOTED ANIMAL 165 NO FINDINGS NOTED ANIMAL 166 NO FINDINGS NOTED ANIMAL 167 NO FINDINGS NOTED ANIMAL 168 NO FINDINGS NOTED ANIMAL 169 NO FINDINGS NOTED ANIMAL 170 NO FINDINGS NOTED ANIMAL 171 NO FINDINGS NOTED * (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 0 5 -NOV-9 8 ) (SCHEDULED NECROPSY. 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) 278 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS ' BACK TO MAIN MAC-IND-22 25-FEB-99 MACROSCOPIC FINDINGS FEMALES GROUP 5 (PFHS) ANIMAL 173 NO FINDINGS NOTED ANIMAL 174 NO FINDINGS NOTED ANIMAL 175 NO FINDINGS NOTED (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) 279 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ '' 'PFHS/PFOS " BACK TO MAIN MAC-IND -23 25-FEB-99 MACROSCOPIC FINDINGS FEMALES GROUP 6 (PFOS) ANIMAL 177 NO FINDINGS NOTED ANIMAL 178 . NO FINDINGS NOTED ANIMAL 179 . NO FINDINGS NOTED ANIMAL 180 NO FINDINGS NOTED ANIMAL 181 NO FINDINGS NOTED ANIMAL 182 NO FINDINGS NOTED ANIMAL 183 . NO FINDINGS NOTED ANIMAL 184 NO FINDINGS NOTED ANIMAL 185 NO FINDINGS NOTED ANIMAL 186 NO FINDINGS NOTED ANIMAL 187 NO FINDINGS NOTED . (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) * 280 NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS `' BACK TO MAIN MAC-IND -24 25-FEB-99 MACROSCOPIC FINDINGS FEMALES GROUP 6 (PFOS) ANIMAL 189 NO FINDINGS NOTED ANIMAL 190 NO FINDINGS NOTED ANIMAL 191 NO FINDINGS NOTED . (SCHEDULED NECROPSY, 0 4 -DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY. 0 4 -DEC-98) 281 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS MALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. BRAIN HEART 1 284 2 332 3 348 4 270 5 303 6 327 7 351 8 301 1.95 2.04 2.03 2.00 1.92 2.01 2.14 1.99 1.081 1.390 1.108 1.070 1.141 1.088 1.123 1.059 LIVER 8.04 10.76 10.40 8.54 10.49 10.38 11.92 9.84 OW-IND - X 30-MAR-99 KIDNEYS ADRENALS SPLEEN TESTES 1.84 2.59 2.41 1.75 2.54 2.06 2.63 2.59 0.038 0.050 0.037 0.040 0.053 0.044 0.051 0.047 0.434 0.565 0.563 0.482 0.488 0.733 0.766 0.605 2.71 2.79 3.32 2.53 2.91 2.82 3.03 2.82 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. BRAIN HEART 17 322 18 295 19 312 20 299 21 315 22 348 23 313 24 312 1.99 2.01 2.00 1.96 1.84 2.05 1.87 1.95 1.149 1.300 0.935 1.033 1.129 1.309 0.972 1.241 LIVER 10.16 11.71 9.71 9.50 10.48 10.71 10.15 10.42 KIDNEYS ADRENALS SPLEEN TESTES 2.35 2.57 2.35 2.24 2.40 2.57 2.12 2.09 0.049 0.050 0.053 0.055 0.050 0.049 0.072 0.049 0.465 0.551 0.541 0.540 0.565 0.712 0.560 0.590 3.05 3.27 3.19 3.21 2.63 3.18 2.87 2.96 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. BRAIN HEART 33 313 1.97 34 288 1.93 35 293 1.95 36 293 1.94 37 303 2.02 38 324 1.98 39 319 - 1.99 40 304 1.82 1.127 0.943 1.131 0.972 1 .3 2 1 1.079 1.077 1.122 LIVER 9.89 8.88 8.71 8.90 9.66 10.40 9.48 8.95 KIDNEYS ADRENALS SPLEEN TESTES 2.08 2.16 2.08 2.14 2.50 2.37 2.19 2.12 0.053 0.046 0.038 0.059 0.058 0.056 0.046 0.056 0.637 0.498 0.476 0.530 0.603 0.592 0.656 0.547 2.52 2.77 2.77 2.74 2.84 2.97 2.69 2.94 GROUP 4 ( ) ANIMAL BODY W. BRAIN NUMBER 49 290 50 296 51 342 52 324 53 320 54 330 55 284 56 263 1.96 2.07 2 .11 1.97 1.98 1.99 1.97 1.93 HEART 1.055 1.193 1.156 1.224 1.331 1.207 1.025 1.043 LIVER 8.23 9.16 10.83 9.52 9.39 9.53 8.50 7.18 KIDNEYS ADRENALS SPLEEN TESTES 1.99 2.08 2.54 2.26 2.36 2.25 2.07 1.73 0.046 0.033 0.046 0.053 0.042 0.041 0.045 0.058 0.488 0.627 0.719 0.559 0.629 0.547 0.537 0.519 3.18 3.00 2.77 2.96 2.58 2.70 2.95 2.75 282 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS MALES GROUP 5 (PFHS) ANIMAL NUMBER BODY W. BRAIN HEART 65 66 67 68 69 70 . 71 72 352 270 297 297 287 347 311 306 2.02 1.89 2.03 1.94 1.86 2.07 1.83 2.04 1.267 0.992 1.131 1.148 1.096 1.153 1.030 1.040 GROUP 6 (PFOS) ANIMAL NUMBER BODY W. BRAIN 81 303 82 295 83 308 84 318 85 362 86 293 87 351 88 317 1.99 1.98 2.06 2.00 1.96 1.93 1.97 2.07 HEART 1.102 1.165 0.961 1.171 1.223 1.259 1.163 1.004 LIVER 16.85 11.52 13.86 13.10 12.96 14.49 11.84 15.62 LIVER 11.04 11.17 10.67 11.52 13.46 12.90 12.33 11.19 OW-IND - 2 30-MAR-9 9 KIDNEYS ADRENALS SPLEEN TESTES 2.77 2.14 2.49 2.31 2.11 2.47 2.19 2.28 0.037 0.038 0.059 0.046 0.058 0.057 0.051 0.056 0.595 0.504 0.549 0.541 0.570 0.745 0.516 0.539 3.22 0.85 3.18 3.07 2.82 3.18 3.05 3.04 KIDNEYS ADRENALS SPLEEN TESTES 2.15 2.21 1.91 2.42 2.34 2.28 2.26 1.96 0.039 0.055 0.039 0.032 0.060 0.039 0.067 0.040 0.501 0.433 0.560 0.510 0.692 0.664 0.634 0.469 2.80 2.86 2.82 2.79 2.88 2.58 3.22 2.84 283 BACK TO MAIN NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS {%) AFTER 4 WEEKS HALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN 1 284 2 332 3 348 4 270 5 303 6 327 "7 351 8 301 0.69 0.61 0.58 0.74 0.63 0.62 0.61 0.66 0.381 0.419 0.318 0.396 0.377 0.333 0.320 0.352 2.83 3.24 2.99 3.16 3.46 3.18 3.40 3.27 0.65 0.78 0.69 0.65 0.84 0.63 0.75 0.86 0.013 0.015 0.011 0.015 0.017 0.013 0.015 0.016 0.153 0.170 0.162 0.179 0.161 0.224 0.218 0.201 OW-IND - 3 3 0 - M A R - 99 TESTES 0.95 0.84 0.95 0.94 0.96 0.86 0.86 0.94 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART 17 322 18 295 19 312 20 299 21 315 22 348 23 313 24 312 0.62 0.68 0.64 0.65 0.58 0.59 0.60 0.62 0.357 0.441 0.300 0.345 0.358 0.376 0.311 0.398 LIVER 3.15 3.97 3.11 3.18 3.33 3.08 3.24 3.34 KIDNEYS ADRENALS SPLEEN TESTES 0.73 0.87 0.75 0.75 0.76 0.74 0.68 0.67 0.015 0.017 0.017 0.018 0.016 0.014 0.023 0.016 0.144 0.187 0.173 0.181 0.179 0.205 0.179 0.189 0.95 1 .1 1 1.02 1.07 0.83 0.91 0.92 0.95 . . GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART 33 313 34 288 35 293 36 293 37 303 38 324 39 319 40 304 0.63 0.67 0.66 0.66 0.67 0.61 0.62 0.60 0.360 0.327 0.386 0.332 0.436 0.333 0.338 0.369 LIVER 3.16 3.08 2.97 3.04 3 .1 9 3.21 2.97 2.94 KIDNEYS ADRENALS SPLEEN TESTES 0.66 0.75 0.71 0.73 0.83 0.73 0.68 0.70 0.017 0.016 0.013 0.020 0.019 0.017 0.014 0.018 0.204 0.173 0.162 0.181 0.199 0.183 0.206 0.180 0.80 0.96 0.95 0.94 0.94 0.92 0.84 0.97 GROUP 4 ( ) ANIMAL BODY W. BRAIN NUMBER (GRAM) 49 290 50 296 51 342 52 324 53 320 54 330 55 284 56 263 0.67 0.70 0.62 0.61 0.62 0.60 0.69 0.73 HEART 0.364 0.403 0.338 0.378 0.416 0.366 0.361 0.397 LIVER 2.84 3.09 3.17 2.94 2.93 2.89 2.99 2.73 KIDNEYS ADRENALS- SPLEEN TESTES 0.69 0.70 0.74 0.70 0.74 0.68 0.73 0.66 0.016 0.011 0.013 0.016 0.013 0.012 0.016 0.022 0.168 0.212 0.210 0.173 0.197 0.166 0.189 0.197 1.10 1.0 1 0.81 0.91 0.81 0.82 1.04 1.05 284 BACK TO MAIN NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS MALES GROUP 5 (PFHS) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER 65 352 66 270 67 297 68 297 69 287 70 347 71 311 72 306 0.57 0.70 0.68 0.65 0.65 0.60 0.59 0.67 0.360 0.367 0.381 0.387 0.382 0.332 0.331 0.340 4.79 4.27 4.67 4.41 4.52 4.18 3.81 5.10 OW-IND - 4 3 0 - M A R - 99 KIDNEYS ADRENALS SPLEEN TESTES 0.79 0.79 0.84 0.78 0.73 0.71 0.70 0.74 0.011 0.014 0.020 0.015 0.020 0.016 0.016 0.018 0.169 0.187 0.185 0.182 0.199 0.215 0.166 0.176 0.91 0.31 1.07 1.03 0.98 0.92 0.98 0.99 GROUP 6 (PFOS) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART 81 303 82 295 83 308 84 318 85 362 86 293 87 351 88 317 0.66 0.67 0.67 0.63 0.54 0.66 0.56 0.65 0.364 0.395 0.312 0.368 0.338 0.430 . 0.331 0.317 LIVER 3.64 3.79 3.46 3.62 3.72 4.40 3.51 3.53 KIDNEYS ADRENALS SPLEEN TESTES 0.71 0.75 0.62 0.76 0.65 0.78 0.64 0.62 0.013 0.019 ' 0.013 0.010 0.017 0.013 0.019 0.013 0.165 0.147 0.182 0.160 0.191 0.227 0.181 0.148 0.92 0.97 0.91 0.88 0.80 0.88 0.92 0.90 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS FEMALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. BRAIN HEART 97 201 98 194 99 204 100 217 101 241 102 229 103 183 104 242 1.93 1.92 1.89 1.96 1.91 1.89 1.70 1.85 0.861 0.799 0.750 0.869 0.917 0.901 0.855 0.867 LIVER 6.95 6.34 6.13 6.82 7.19 6.61 5.79 7.13 OW-IND - 5 3 0 - M A R - 99 KIDNEYS ADRENALS SPLEEN OVARIES 1.44 1.39 1.35 1.56 1.71 1.49 1.36 1.71 0.060 0.075 0.052 0.057 0.046 0.065 0.062 0.061 0.424 0.345 0.398 0.483 0.458 0.366 0.426 0.481 0.089 0.142 0.128 0.119 0.130 0.163 0.100 0.122 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. BRAIN HEART 113 204 114 246 115 215 116 218 117 194 118 200 119 201 120 213 1.91 2.03 1.92 2.08 1.82 1.89 1.93 1.88 0.890 0.883 0.785 0.827 0.749 0.829 0.751 0.786 LIVER 6.96 8.13 7.39 7.59 6.97 6.78 6.35 7.29 KIDNEYS ADRENALS SPLEEN OVARIES 1.47 1.85 1.75 1.44 1.68 1.59 1.48 1.75 0.055 0.056 0.078 0.062 0.073 0.054 0.067 0.069 .470 0.479 0.501 0.378 0.433 0.435 0.446 0.457 0.129 0.130 0.123 0.121 0.172 0.144 0.104 0.142 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. BRAIN HEART 129 204 ___ 130 182 1.84 131 196 1.86 132 205 ' 1.88 133 1S1 1.77 134 215 1.92 135 227 1.85 136 193 1.79 ___ 0.744 0.736 0.847 0.770 0.799 0.917 0.714 LIVER -- 5.70 6.48 6.23 6.19 6.84 7.07 6.09 KIDNEYS ADRENALS SPLEEN OVARIES ... 1.42 1.61 1.59 1.36 1.57 1.62 1.41 - - 0.052 0.053 0.060 0.054 0.063 0.077 0.060 ---- 0.298 0.300 0.479 0.454 0.494 0.4870.409 ---- 0.127 0.162 0.138 0.125 0.127 0.146 0.118 GROUP 4 ( ) ANIMAL NUMBER BODY W. BRAIN 145 193 146 222 147 223 148 186 149 220 150 219 151 200 152 201 1.80 1.89 2.01 1.84 1.96 2.00 1.84 1.85 HEART 0.794 0.961 0.794 0.767 0.855 0.773 0.778 0.719 LIVER 6.44 6.76 6.05 5.72 8.10 6.71 6.22 6.28 KIDNEYS ADRENALS SPLEEN OVARIES 1.53 1.46 1.64 1.50 1.89 1.48 1.41 1.33 0.066 0.160 0.057 0.043 0.055 0.068 0.049 0.061 1.527 0.529 0.511 0.506 0.420 0.478 0.463 0.429 0.131 0.055 0.138 0.116 0.156 0.130 0.136 0.129 286 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS FEMALES GROUP 5 (PFHS) ANIMAL NUMBER BODY W. BRAIN HEART iei 193 162 201 163 191 164 202 165 198 166 208 167 183 168 228 1.94 1.93 1.78 1.78 1.81 1.98 1.86 1.99 0.769 0.703 0.816 0.800 0.910 0.881 0.688 0.880 GROUP 6 (PFOS) ANIMAL NUMBER BODY W. BRAIN 177 215 178 163 179 185 180 173 181 185 182 199 183 191 184 188 1.86 1.79 1.91 1.86 1.88 1.96 1.91 1.88 HEART 0.788 0.660 0.663 0.673 0.818 0.845 0.777 0.757 LIVER 7.96 6.70 5.97 6.57 7.08 6.67 5.68 7.08 LIVER 9.28 6.47 7.23 7.16 7.62 8.41 7.85 8.36 OW-IND - 6 30-MAR-99 KIDNEYS ADRENALS SPLEEN OVARIES 1.61 1.42 1.32 1.68 1.46 1.48 1.47 1.62 0.068 0.056 0.060 0.047 0.071 0.062 0.049 0.052 0.436 0.419 0.397 0.443 0.361 0.431 0.357 0.415 0.150 0.102 0.121 0.138 0.138 0.152 0.115 0.141 KIDNEYS ADRENALS SPLEEN OVARIES 1.69 1.29 1.48 1.41 1.39 1.47 1.43 1.52 0.053 0.044 0.047 0.061 0.055 0.059 0.059 0.057 0.474 0.327 0.386 0.394 0.404 0.420 0.442 0.450 0 .111 0.135 0.108 0.115 0.146 0.110 0.154 0.148 287 BACK TO MAIN NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS FEMALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER 97 201 98 194 99 204 100 217 101 241 102 229 103 183 104 242 0.96 0.99 0.93 0.90 0.79 0.83 0.93 0.76 0.428 0.412 0.368 0.400 0.380 0.393 0.467 0.358 3.46 3.27 3.01 3.14 2.98 2.89 3.16 2.95 OW-IND - 7 3 O-MAR-9 9 KIDNEYS ADRENALS SPLEEN OVARIES 0.71 0.71 0.66 0.72 0.71 0.65 0.74 0.71 0.030 0.039 0.025 0.026 0.019 0.028 0.034 0.025 0.211 0.178 0.195 0.223 0.190 0.160 0.233 0.199 0.044 0.073 0.063 0.055 0.054 0.071 0.055 0.050 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART 113 204 114 246 115 215 116 218 117 194 118 200 119 201 120 213 0.94 0.83 0.89 0.95 0.94 0.95 0.96 0.88 0.436 0.359 0.365 0.379 0.386 0.415 0.374 0.369 LIVER 3.41 3.31 3.44 3.48 3.59 3.39 3.16 3.42 KIDNEYS ADRENALS SPLEEN OVARIES 0.72 0.75 0.81 0.66 0.87 0.80 0.73 0.82 0.027 0.023 0.036 0.028 . 0.038 0.027 0.033 0.032 0.230 0.195 0.233 0.173 0.223 0.218 0.222 0.215 0.063 0.053 0.057 0.056 0.089 0.072 0.052 0.067 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART 129 204 130 182 131 196 132 205 133 181 134 215 135 227 136 193 1.0 1 0.95 0.92 0 .9 8 0.89 0.81 0.93 0.409 0.376 0.413 0 .425 0.372 0.404 0.370 LIVER 3.13 3.31 3.04 3 .4 2 3.18 3.11 3.16 KIDNEYS ADRENALS SPLEEN OVARIES 0.78 0.82 0.78 0 .75 0.73 0.71 0.73 0.029 0.027 0 .0 2 9 0 .0 3 0 0.029 0.034 0.031 0.164 0.153 0 .2 3 4 0.251 0.230 0.215 0.212 0.070 0.083 0 .0 67 0.069 0.059 0.064 0.061 GROUP 4 ( ) ANIMAL NUMBER BODY W. (GRAM) BRAIN 145 193 146 222 147 223 148 186 149 220 150 219 151 200 152 201 0.93 0.85 0.90 0.99 0.89 0.91 0.92 0.92 HEART 0.411 0.433 0.356 0.412 0.389 0.353 0.389 0.358 LIVER 3.34 3.04 2.71 3.07 3.68 3.06 3.11 3.12 KIDNEYS ADRENALS SPLEEN OVARIES 0.79 0.66 0.73 0.80 0.86 0.68 0.70 0.66 0.034 0.072 0.026 0.023 0.025 0.031 0.025 0.030 0.791 0.238 0.229 0.272 0.191 0.218 0.232 0.213 0.068 0.025 0.062 0.062 0.071 0.059 0.068 0.064 288 BACK TO MAIN NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS FEMALES GROUP 5 (PFHS) an im al NUMBER BODY W. (GRAM) BRAIN HEART LIVER 16 1 193 16 2 201 163 191 1 6 4 202 165 198 16 6 208 16 7 183 168 228 1.01 0 .96 0 .9 3 0.88 0 .92 0 .95 1.01 0 .87 0 .3 9 8 0 .350 0 .42 7 0 .396 0 .4 6 0 0 .42 4 0 .3 76 0 .386 4 .13 3 .33 3 .13 3 .2 5 3 .5 7 3 .2 1 3 .10 3 .10 GROUP 6 (PFOS) ANIMAL NUMBER BODY W. (GRAM) BRAIN 177 178 179 180 . 181 182 183 184 215 163 185 173 185 199 191 188 0.86 1.10 1.0 3 1.0 7 1.02 0 .9 9 1.00 1.00 HEART 0 .3 67 0.40 5 0 .358 0 .389 0 .442 0 .42 5 0 .40 7 0 .4 0 3 LIVER 4.3 2 3 .9 7 3 .9 1 4 .14 4 .12 4 .2 3 4 .11 4 .4 5 O W -IN D - 8 3 0-M A R -9 9 KIDNEYS ADRENALS SPLEEN OVARIES 0 .8 3 0 .70 0 .69 0 .8 3 0 .74 0 .71 0 .8 0 0 .71 0 .0 3 5 0 .0 2 8 0 .0 31 0 .0 2 3 0 .0 3 6 0 .0 3 0 0.0 27 0 .0 2 3 0 .226 0 .2 0 8 0 .2 0 8 0 .219 0 .182 0.20 7 0 .19 5 0 .182 0 .0 7 8 0 .0 51 0 .0 63 0 .0 6 8 0.0 70 0.0 73 0 .0 6 3 0 .0 6 2 . KIDNEYS ADRENALS SPLEEN OVARIES 0 .79 0 .79 0 .8 0 0 .8 2 0 .75 0 .74 0 .75 0 .8 1 0 .0 2 5 0 .0 2 7 0 .0 2 5 0 .0 3 5 0 .0 3 0 0 .0 3 0 0 .0 31 0 .0 3 0 0.220 0.201 0 .2 0 9 0 .228 0 .218 0.211 0 .2 3 1 0 .239 0 .0 52 0 .0 8 3 0.0 58 0 .0 66 0 .0 79 0 .0 55 0 .0 8 1 0 .0 79 28 9 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 2 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BOOT W. BRAIN HEART 9 344 10 277 11 338 2.0 3 1.8 1 2.01 1.18 5 0 .960 1.12 8 LIVER 8 .98 7.4 2 8 .32 O W -IN D - 9 3 0 -M A R -99 KIDNEYS ADRENALS SPLEEN TESTES 2.02 1.7 4 2 .3 5 0 .0 3 9 0 .0 37 0 .0 5 0 0 .440 0 .358 0 .526 2.88 2 .5 5 3 .0 1 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BOOT W. BRAIN HEART 25 328 26 295 27 320 1.9 3 1.9 6 2.0 5 1.0 0 7 1.0 68 1.112 LIVER 8 .62 7.5 4 8 .65 KIDNEYS ADRENALS SPLEEN 2 .0 4 2.11 2 .0 9 0 .0 41 0 .046 0 .0 31 0 .578 0 .5 71 0 .555 2 .6 7 2 .6 2 1.10 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BOOT W. BRAIN HEART LIVER 41 379 42 319 43 334 2.01 2 .0 4 1.8 0 1 .1 1 3 1.2 8 4 1 .119 12.10 8.88 8 .7 1 GROUP 4 ( \ ANIMAL NUMBER BOOT W. BRAIN 57 4 15 ' 58 354 59 329 2 .13 2.02 1 .9 1 HEART 1.12 5 1.17 9 1.15 5 LIVER 11 .4 1 10 .4 5 9 .0 5 KIDNEYS ADRENALS SPLEEN TESTES 2 .75 2 .15 2 .3 3 0.0 47 0 .0 36 0.0 47 0 .5 0 4 0 .578 0 .547 3 .16 3 .19 2 .9 1 KIDNEYS ADRENALS SPLEEN TESTES 2 .56 2 .5 0 2.11 0 .0 41 0 .0 6 3 0 .0 6 2 0 .739 0 .582 0 .548 3 .53 3 .3 5 3 .18 GROUP 5 (PFHS) ANIMAL NUMBER BOOT W. BRAIN 73 386 74 249 75 336 2.00 1.7 3 2 .0 6 HEART 1.3 0 9 1.0 2 7 1.112 LIVER 12 .15 6 .8 9 9 .6 2 KIDNEYS ADRENALS s p l e e n TESTES 2 .54 1.6 0 2 .4 1 0 .0 4 5 0 .0 3 5 0 .0 4 3 0 .6 3 0 0 .476 0 .5 17 3 .2 7 1.3 4 3 .2 1 290 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 2 WEEKS RECOVERY MALES GROUP 6 (PFOS) ANIMAL NUMBER BODY W. BRAIN HEART 89 394 90 370 91 377 2 .03 2 .0 7 2.00 1.3 3 3 1.2 3 7 1.3 2 8 O W - I N D - 10 3 0 - M A R - 99 * LIVER KIDNEYS ADRENALS SPLEEN TESTES 14 .2 0 2.66 14 .6 2 2 .58 13 .3 9 2 .70 0 .0 41 0 .0 4 8 0 .0 54 0 .758 0 .6 3 1 0 .5 17 3 .3 1 2 .8 0 2 .9 7 291 BACK TO MAIN NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS (%) AFTER 2 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. (GRAM) BRAIN REART LIVER 9 344 10 2 77 11 338 0 .59 0 .65 0 .6 0 0 .344 0 .347 0 .334 2 .6 1 2.68 2 .4 6 . GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART 25 328 26 295 27 320 0 .59 0 .67 0 .64 0 .30 7 0 .362 0 .348 LIVER 2 .63 2 .5 6 2 .70 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER 41 379 42 319 43 334 0 .53 0 .64 0 .54 0 .294 0 .4 0 3 0 .335 3 .19 2 .78 2 .6 1 GROUP 4 ( ) ANIMAL NUMBER BODY W. (GRAM) BRAIN 57 415' 58 354 59 329 0 .5 1 0 .57 0 .58 HEART 0 .2 71 0 .333 0 .3 51 LIVER 2 .7 5 2 .9 5 2 .75 GROUP 5 (PFHS) ANIMAL NUMBER BODY H. (GRAM) BRAIN 73 386 74 249 75 336 0 .52 0 .6 9 0 .6 1 HEART 0 .339 0 .412 0 .3 3 1 LIVER 3 .15 2 .7 7 2.86 O W - I N D - 11 3 O-MAR-99 * KIDNEYS ADRENALS SPLEEN TESTES 0 .59 0 .63 0 .70 0.011 0.0 13 0 .0 15 0 .128 0 .129 0 .156 0 .8 4 0 .92 0 .8 9 KIDNEYS ADRENALS SPLEEN TESTES 0 .62 0 .72 0 .65 0 .0 13 0 .0 16 0.010 0 .176 0 .194 0 .173 0 .8 1 0 .89 0 .3 4 KIDNEYS ADRENALS s p l e e n TESTES 0 .73 0 .6 7 0 .70 0.012 0.011 0 .0 14 0 .133 0 .18 1 0 .164 0 .8 3 1.00 0 .8 7 > KIDNEYS ADRENALS SPLEEN TESTES 0 .62 0 .7 1 0 .64 0.010 0 .0 18 0 .0 19 0 .178 0 .164 0 .16 7 0 .8 5 0 .95 0 .9 7 KIDNEYS ADRENALS SPLEEN TESTES 0.66 0 .64 0 .72 0.012 0 .0 14 0 .013 0 .16 3 . 0 .8 5 0 .19 1 0 .54 0 .154 0 .95 292 BACK TO MAIN NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS (%) AFTER 2 WEEKS RECOVERY HALES GROUP' 6 (PFOS) ANIMAL NUMBER BOOT W. (GRAM) BRAIN HEART LXVER 89 394 90 370 91 377 0 .52 0 .5 6 0 .53 0 .338 0 .334 0 .352 3 .6 0 3 .9 5 3 .5 5 O W - I N D - 12 3 0 - M A R - 99 * KIDNEYS ADRENALS s p l e e n TESTES 0.68 0 .70 0 .72 0.010 0 .0 13 0 .0 14 0 .192 0 .17 1 0 .13 7 0 .8 4 0 .76 0 .79 293 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 2 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. BRAIN HEART 105 217 106 272 107 227 1.9 8 2 .0 5 1.8 5 0 .8 6 7 0 .960 0.868 LIVER 6 .95 9 .23 6 .9 0 O W -IN D - 13 3 O -M A R -9 9 KIDNEYS ADRENALS SPLEEN OVARIES 1.5 1 1 .7 1 1.3 9 0 .0 52 0.0 56 0 .0 57 0 .449 0 .439 0 .372 0 .150 0 .574 0 .132 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. BRAIN HEART 1 2 1 200 122 203 123 231 1.8 5 1.8 5 1.9 8 0 .8 12 0 .9 15 0 .8 4 8 LIVER 6 .72 6.01 7.7 2 KIDNEYS ADRENALS SPLEEN OVARIES 1.6 0 1 .3 1 1.6 2 0.0 74 0.0 57 0 .0 57 0 .3 67 0 .3 7 0 0 .536 0 .12 5 0 .13 5 0 .13 1 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. BRAIN HEART LIVER 137 217 138 189 1 3 9 200 1.88 1.9 1 1.9 4 0 .873 0 .76 7 0 .853 7.4 0 5 .6 6 6 .0 6 KIDNEYS ADRENALS SPLEEN OVARIES 1.7 0 1.4 1 1.4 4 0 .0 8 0 0.0 47 0.0 67 0 .5 2 3 0 .382 0 .3 4 4 0.120 0.120 0 .138 GROUP 4 ( ) ANIMAL NUMBER BODY W. BRAIN 153 2 14 ' 154 246 155 191 1.8 9 1.9 5 1.9 0 HEART 0 .9 8 0 0 .9 5 0 0 .878 LIVER KIDNEYS ADRENALS SPLEEN OVARIES 7.0 5 7.0 0 7 .18 ' 1.6 1 1.7 2 1.4 8 0 .0 75 0 .0 6 5 0 .0 6 8 0 .4 0 4 0 .4 3 2 0 .4 0 5 0 .14 9 0 .16 2 0 .12 3 GROUP 5 (PFHS) ANIMAL NUMBER BODY W. BRAIN 169 228 170 211 1 7 1 232 1.9 0 1.9 2 L . 95 HEART 0 .844 0 .78 4 0 .8 9 2 LIVER 6 .77 6.8 4 7.9 6 KIDNEYS ADRENALS SPLEEN OVARIES 1.5 4 1.5 5 1.4 5 0 .0 61 0 .0 55 0 .059 0 .3 6 2 0 .5 0 8 0 .476 0 .13 5 0 .118 0 .14 7 294 BACK TO MAIN NOTOX Project 242933 ___ ORGAN WEIGHTS (GRAM) AFTER 2 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) ANIMAL NUMBER BODY W. BRAIN HEART 185 197 186 199 18 7 203 2.02 1.9 3 2.0 4 0.70 2 0 .73 5 0 .732 LIVER 8 .18 7 .10 7.3 7 OW-IND - 14 3O-MAR-9 9 * KIDNEYS ADRENALS SPLEEN OVARIES 1.4 8 1.4 1 1.2 6 0 .0 57 0.0 56 0 .0 5 5 0 .397 0 .4 15 0 .365 0 .138 0 .132 0 .12 7 295 BACK TO MAIN NOTOX Project 242933 _ ORGAN/BODY WEIGHT RATIOS (%) AFTER 2 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. BRAIN (GRAM) ' HEART LIVER 105 217 106 272 107 227 0 .9 1 0 .75 0 .8 1 0 .4 0 0 0 .353 0 .3 8 2 3 .2 0 3 .39 3 .0 4 O W - I N D - 15 30-MAR-99 - KIDNEYS ADRENALS SPLEEN OVARIES 0 .69 0 .6 3 0 .6 1 0 .0 2 4 0.021 0 .0 2 5 0.20 7 0 .16 1 0 .164 0 .0 69 0.211 0.0 58 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART 1 2 1 200 122 203 123 231 0 .9 3 0 .9 1 0.86 0 .406 0 .4 5 1 0 .367 LIVER KIDNEYS ADRENALS SPLEEN OVARIES 3 .3 6 0 .8 0 2 .9 6 0 .65 3 .3 4 0 .70 0 .0 37 0 .0 2 8 0 .0 2 5 0 .18 4 0 .182 0 .2 3 2 0.0 63 0 .0 67 0 .0 57 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER 137 217 138 189 1 3 9 200 0 .87 1.01 0 .97 0 .4 0 2 0 .4 0 6 0 .42 7 3 .4 1 3.0 0 3.0 3 KIDNEYS ADRENALS SPLEEN OVARIES 0 .79 0 .75 0 .72 0 .0 3 7 0 .0 2 5 0 .0 3 4 0 .2 4 1 0.202 0 .172 0 .0 55 0 .0 63 0 .0 69 GROUP 4 ( ) ANIMAL NUMBER BODY W. (GRAM) BRAIN 153 214' 154 246 155 191 0.88 0 .79 0 .99 HEART 0 .458 0 .3 8 6 0 .4 6 0 LIVER 3 .2 9 2 .8 5 3 .76 KIDNEYS ADRENALS SPLEEN OVARIES 0 .75 0 .70 0 .77 0 .0 3 5 0 .0 2 6 0 .0 3 6 0 .18 9 0 .176 0.212 0.0 70 0 .0 66 0 .064 GROUP 5 (PFHS) ANIMAL NUMBER BODY W. (GRAM) BRAIN 169 228 170 211 1 7 1 232 0 .8 3 0 .9 1 0 .8 4 HEART 0 .3 7 0 0 .372 0 .384 LIVER 2 .9 7 3 .2 4 3 .4 3 KIDNEYS ADRENALS SPLEEN OVARIES 0.68 0 .74 0 .62 0 .0 2 7 0 .0 2 6 0 .0 2 5 0 .159 0 .24 1 0.20 5 0 .0 59 0 .0 56 0 .0 63 296 BACK TO MAIN NOTOX Project 242933 _^ ORGAN/BODY WEIGHT RATIOS (%} AFTER 2 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER 185 197 186 199 18 7 203 1.02 0 .97 1.00 0 .3 56 0 .369 0 .3 6 1 4 .15 3 .5 7 3 .6 3 O W - I N D - 16 30-MAR-99 * KIDNEYS ADRENALS SPLEEN OVARIES 0 .75 0 .71 0 .62 0 .0 2 9 0 .0 2 8 0.0 27 0.202 0.20 9 0 .18 0 0.0 70 0 .0 6 6 0 .0 63 257 BACK TO MAIN NOTOX P r o j e c t 24 2 933 ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. BRAIN HEART 13 415 14 360 15 392 1.9 7 1.8 3 2.12 1.2 8 4 1.2 0 6 1 .0 9 9 - LIVER 1 2 .2 6 9 .5 7 10 .4 7 OW-IND - 17 3 0 - M A R - 99 KIDNEYS ADRENALS SPLEEN TESTES 2 .6 9 2 .38 2 .24 0 .0 3 8 0 .0 41 0 .058 0 .576 0 .6 11 0 .656 2.86 2 .73 3 .0 1 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. BRAIN HEART 29 330 30 396 3 1 362 32 2.01 2 .0 8 2 .0 8 1.19 5 1.19 9 1.2 2 8 LIVER 9 .4 1 10 .2 7 9 .6 6 KIDNEYS ADRENALS SPLEEN TESTES 2 .3 5 2 .4 8 2 .4 4 0 .0 4 8 0 .0 59 0 .0 4 2 0 .456 0 .544 0 .6 2 0 ** "* 2 .9 7 2 .92 3 .6 8 ---- GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. BRAIN HEART LIVER 45 380 46 380 47 375 2.0 7 2.0 7 2.12 1.2 8 2 1.2 3 7 1.13 2 11.0 8 9 .77 10 .0 5 KIDNEYS ADRENALS SPLEEN TESTES 2 .5 5 2 .4 8 2 .50 0 .0 5 0 0 .0 5 6 0.0 57 0 .656 0 .534 0 .582 3 .0 6 3 .11 3 .17 GROUP 4 ( ) ANIMAL NUMBER BODY W. BRAIN 61 434 62 406 63 363 2 .3 3 1.9 9 2.00 HEART 1.3 78 1.3 8 9 1.12 9 LIVER 9 .9 1 10 .4 6 9 .8 1 KIDNEYS ADRENALS SPLEEN TESTES 2 .79 2 .32 2 .6 7 0 .0 7 0 0 .0 4 6 0 .0 51 0 .692 0 .70 5 0 .76 2 3 .3 6 2 .9 7 2 .9 8 GROUP 5 (PFHS) ANIMAL NUMBER BODY W. BRAIN 77 358 78 321 79 326 2.11 1.9 5 1.9 9 HEART 1.10 5 0 .8 75 1.16 4 LIVER 9 .5 7 8 .43 8 .54 KIDNEYS ADRENALS SPLEEN TESTES 2 .13 1.8 9 2 .6 9 0 .0 5 0 0 .0 45 0.0 47 0 .625 0 .529 0 .5 0 2 3 .2 2 3.0 3 3 .0 1 298 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS RECOVERY MALES GROUP 6 (PFOS) ANIMAL BODY W. BRAIN NUMBER HEART 93 301 94 374 95 356 1.96 2.08 1.98 0.953 1.429 1.311 ZfjTVER 8.98 12.66 11.87 OW-IND - 18 30-MAR-9 9 KIDNEYS ADRENALS SPLEEN TESTES 2.26 2.66 2.53 0.055 0.037 0.047 0.505 0.774 0.607 3.10 3.62 3.09 299 BACK TO MAIN NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS RECOVERY HALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER 13 415 14 360 15 392 0 .47 0 .5 1 0 .54 0 .309 0 .335 0 .2 8 0 2 .9 6 2.66 2 .6 7 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART 29 330 30 396 31 362 32 0 .6 1 0 .52 0 .57 0 .3 6 2 0 .3 0 3 0 .339 LIVER 2 .8 5 2 .59 2 .6 7 OW -IN D - 1 9 3 0 -M A R -99 KIDNEYS ADRENALS SPLEEN TESTES 0 .65 0.66 0 .57 0 .0 0 9 0.011 0 .0 15 0 .139 0 .170 0 .16 7 0 .69 0 .76 0 .77 KIDNEYS ADRENALS SPLEEN TESTES 0 .71 0 .6 3 0 .67 0 .0 15 0 .0 15 0.012 0 .138 0 .13 7 0 .17 1 0 .90 0 .74 1.02 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER 45 380 46 380 47 375 0 .54 0 .55 0 .57 0 .337 0 .326 0 .3 0 2 2 .92 2 .5 7 2.68 ' KIDNEYS ADRENALS SPLEEN 0 .67 0 .65 0 .67 0 .0 13 0 .0 15 0 .0 15 0 .173 0 .14 1 0 .155 TESTES 0 .8 0 0 .82 0 .8 5 GROUP 4 ( ) ANIMAL NUMBER BODY W. (GRAM) BRAIN 61 434 62 406 63 363 0 .54 0 .49 0 .55 HEART 0 .318 0 .3 4 2 0 .3 11 LIVER 2 .28 2 .58 2 .70 KIDNEYS ADRENALS SPLEEN TESTES 0 .64 0 .57 0 .74 0 .0 16 . 0.011 0 .0 14 0 .159 0 .174 0.210 0 .77 0 .73 0 .82 GROUP 5 (PFHS) ANIMAL NUMBER BODY W. (GRAM) BRAIN 77 358 78 321 79 326 0 .59 0 .6 1 0 .6 1 HEART 0 .309 0 .273 0 .3 57 LIVER 2 .6 7 2 .6 2 2 .6 2 KIDNEYS ADRENALS SPLEEN TESTES 0 .5 9 0 .59 0 .8 3 ' 0 .0 14 0 .0 14 0 .0 14 0 .175 0 .16 5 0 .154 0 .90 0 .94 0 .92 300 BACK TO MAIN NOTOX Project 242933 _ ORGAN/BODY WEIGHT RATIOS {%) AFTER 4 WEEKS RECOVERY MALES GROUP 6 (PFOS) ANIMAL NUMBER BODVT W. (GRAMI BRAIN HEART LIVER 93 301 94 374 95 356 0 .65 0 .56 0 .56 0 .3 17 0 .382 0 .3 6 8 2 .9 8 3 .39 3 .3 4 O M - I N D - 20 3 0 - M A R - 99 * KIDNEYS ADRENALS SPLEEN TESTES 0 .75 0 .7 1 0 .7 1 0 .0 18 0.010 0 .0 13 0 .168 0.20 7 0 .17 1 1.0 3 0 .9 7 0 .8 7 301 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. BRAIN HEART 109 212 110 261 111 255 1.86 1.8 7 2.01 0 .8 4 7 1.0 52 0 .850 LIVER 5.8 3 8.12 7.0 9 O W -IN D - 2 1 3 0 -M A R -99 KIDNEYS ADRENALS SPLEEN OVARIES 1.3 4 1.8 4 1.6 0 0 .0 6 2 0 .426 0 .078 0 .508 0 .0 55 - 0.402 0 .13 7 0 .14 4 0 .118 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. BRAIN HEART 125 223 1 2 6 220 127 241 123 2.00 1.8 9 2.00 0 .826 0 .75 7 0 .9 7 1 LIVER 7 .2 1 6.22 7.2 0 KIDNEYS ADRENALS SPLEEN OVARIES 1.6 2 1 .7 2 1.5 3 0 .0 7 9 0 .0 5 3 0 .0 61 0 .430 0 .3 0 9 0 .5 8 9 0.122 0 .13 8 0 .179 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. BRAIN HEART LIVER 1 4 1 212 142 211 143 254 1.8 3 1.86 1.9 4 0 .8 54 0 .834 0 .922 6 .6 9 6 .0 9 7 .7 9 KIDNEYS ADRENALS SPLEEN OVARIES 1.6 4 1.3 8 1.6 1 0 .0 6 8 . 0.053 0 .0 6 6 0 .429 0 .4 8 9 0 .360 0 .160 0 .138 0 .13 6 GROUP 4 ( ) ANIMAL NUMBER BODY W. BRAIN 157 219 158 211 159 275 2.02 1.9 4 1.9 4 HEART 0 .9 0 0 0 .836 1.0 18 LIVER 6 .0 4 6 .82 9 .0 4 KIDNEYS ADRENALS SPLEEN OVARIES 1.4 6 1.6 2 1.9 5 0 .0 61 0 .0 61 0 .0 6 2 0 .595 0 .3 9 1 0 .525 0 .12 5 0 .16 7 0 .20 4 GROUP 5 (PFHS) ANIMAL NUMBER BODY W. BRAIN 173 219 174 241 175 219 1.8 5 1.9 7 1.8 1 HEART 0.866 0 .92 2 0 .793 LIVER 6 .99 6.88 6 .60 KIDNEYS ADRENALS SPLEEN OVARIES 1.7 5 1.5 8 1.5 2 ' 0 .0 6 4 0 .0 6 8 0 .0 6 3 0 .3 77 0 .329 0 .358 0 .12 3 0 .128 0 .20 7 302 BACK TO MAIN NOTOX Project 242933 ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) ANIMAL NUMBER BODY W. BRAIN HEART 189 201 19 0 220 1 9 1 202 1.7 8 1.9 2 1.9 3 0 .854 0 .79 9 0 .8 2 3 LIVER 7.7 2 7 .6 1 7 .15 O W -IN D - 22 3 0 -M A R -99 KIDNEYS ADRENALS SPLEEN OVARIES 1.5 5 1.8 0 1.68 0 .0 6 9 0 .0 4 9 0 .0 5 5 0 .48 5 0 .4 3 1 0 .366 0 .13 9 0 .14 4 0 .14 1 303 BACK TO MAIN NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER 109 212 110 261 111 255 0.88 0 .7 1 0 .79 0 .4 0 0 0 .4 0 3 0 .333 2 .75 3 .11 2 .78 O W -IN D - 23 3 0 -M A R -9 9 KIDNEYS ' ADRENALS SPLEEN OVARIES 0 .6 3 0 .70 0 .6 3 0 .0 2 9 0 .0 3 0 0.022 0.201 0 .19 5 0 .158 0 .0 65 0 .0 55 0 .0 46 GROUP 2 (TELOMER ALCOHOL) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART 125 223 1 2 6 220 127 241 128 0 .90 0.86 0 .8 3 0 .3 7 0 0 .3 4 4 0 .4 0 3 LIVER 3 .23 2 .8 3 2 .9 9 KIDNEYS ADRENALS SPLEEN OVARIES 0 .73 0 .78 0 .64 0 .0 3 5 0 .0 2 4 0.0 25 0 .19 3 0 .140 0 .244 0 .0 55 0 .0 63 0 .0 74 GROUP 3 (TELOMER ACRYLATE) ANIMAL NUMBER BODY W. (GRAM) BRAIN HEART LIVER 1 4 1 212 142 211 143 254 0 .8 7 0.88 0 .76 0 .4 0 3 0 .395 0 .3 6 3 3 .15 2.8 9 3 .0 7 KIDNEYS ADRENALS SPLEEN OVARIES 0 .77 0.66 0 .6 3 0 .0 3 2 0.0 25 0 .026 0.202 0 .2 3 2 0 .142 0 .0 75 0.0 65 0 .0 54 GROUP 4 ( ) ANIMAL NUMBER BODY W. (GRAM) BRAIN 157 219 158 211 159 275 0 .92 0 .92 0 .70 GROUP 5 (PFHS) ANIMAL NUMBER BODY W. (GRAM) BRAIN 173 174175 219 241 219 0 .8 5 0 .8 2 0 .83 HEART 0 .4 11 0 .396 0 .3 7 0 HEART 0 .3 9 5 0 .3 8 3 0 .3 6 2 LIVER KIDNEYS ADRENALS SPLEEN OVARIES 2 .76 3 .2 3 3 .29 0 .6 7 0 .0 2 8 0 .77 0 .029 0 .7 1 0.023 ' 0 .272 0 .18 5 0 .19 1 0 .0 57 0 .0 79 0 .0 74 LIVER KIDNEYS ADRENALS SPLEEN OVARIES 3 .19 2.86 3 .0 2 0 .8 0 0 .65 0 .69 0 .029 0 .0 2 8 0 .029 0 .172 0 .13 7 0 .163 0 .0 56 0 .0 5 3 0.0 95 304 BACK TO MAIN NOTOX Project 242933 ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS RECOVERY FEMALES GROUP 6 (PFOS) ANIMAL BODY W. BRAIN NUMBER .(GRAM) HEART LIVER 189 201 190 220 191 202 0.88 0.87 0.96 0.425 0.363 0.407 3.84 3.46 3.54 O W - I N D - 24 3 0 - M A R - 99 * KIDNEYS ADRENALS SPLEEN OVARIES 0.77 0.82 0.83 0.034 0.022 0.027 0.241 0.196 0.181 0.069 0.065 0.070 305 ProPath GmbH APPENDIX 1 BACK TO MAIN NOTOX Safety & Environmental Research B.V. Pathology Report Exploratory 28-Day Oral Toxicity Study with Telomer Alcohol, Telomer Acrylate, , PFHS and PFOS (positive control) by Daily Gavage in the Rat followed by a 14/28-Day Recovery Period NOTOX Project 242933 NOTOX Substances 83484, 83493, 83502, 84456 and 84141 3M Test no. T-7023, T-7024, T-7022, T-7032, T-6295 This report contains 146 pages Dr. J.Th. Wilson Oberemattstr.52a Pratteln4133 Switzerland tel: +41 61 823 0410 fax:+41 61 8230411 BACK TO MAIN PATHOLOGY REPORT Page 2/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: ' 99010 Date : 21.Apr.1999 Table of Contents Table of contents.................. 2 Authentication.............................................. 3 Summary.......................................................................... 4 Materials and methods....................................................... 4 R e s u l t s ................... 4 Conclusions .......................................................................4 Incidence table - macroscopicfindings .......................................... 4 Incidence table - selected microscopic findings with grades ................... 4 Incidence table - microscopicfindings .......................................... 4 Individual animal microscopic findings .......................................... 4 Animal data l i s t ................................................. 4 Individual animaldata records ................................................... 4 BACK TO MAIN PATHOLOGY REPORT Page 3/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ VPFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Authentication . The undersigned hereby declares, that the histopathology data in this report were compiled by him, and that they reflect accurately the primary data records. This report, consisting of 146 pages, was created by the computer system of Propath GmbH. Jeffrey Th. Wilson, Dr.med.vet, BVSc, MSc, MRCVS Toxicologic Pathologist Propath GUbH CH-4133 Pratteln, Switzerland BACK TO MAIN PATHOLOGY REPORT Page 4/146 Test Article: telomer alcohol/telomer acrylate/ ./pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r .1999 Summary '. - P'athomorphologic examination was performed on.168 Sprague Dawley rats (84 males, 84 females) which had been subjected to an exploratory 28-day oral (gavage) toxicity study with the test articles Telomer alcohol/ Telomer acrylate/ ,/ PIUS/ PFOS. The rats were assigned to' six dose groups each containing fourteen males and fourteen females. Test articles were administered once daily by gavage at doses of 30 (telomer alcohol, telomer acrylate and ), 10 (PFHS) and 3 mg/kg (PFOS) (dose groups 2, 3, 4, 5 and 6 respectively) for 28 days. The rats of the control group 1 received the vehicle, 1% aqueous carboxymethyl cellulose, alone. At the end of the treatment period eight animals of each sex from all groups were killed and subjected to complete necropsies (main study). Three males and three females from each dose group were subjected to necropsy following 14-day or 28-day treatment-free periods (recovery allocation 1 and 2). All rats were necropsied. Histopathologic examination was performed on adrenal glands, kidneys, liver, lymph nodes - mandibular and mesenteric, ovaries, pancreas, spleen, testes, thymus and all organs or tissues with macroscopic findings. . All animals survived their allocated treatment periods. Hepatocellular hypertrophy was present in group 2 (30 mg/kg/day telomer alcohol), group 5 (10 mg/kg/day PFHS) males and in both sexes of group 6 (3 mg/kg/day PFOS) at the end of the main study. In the pancreas a slight increase in the incidence of exocrine apoptosis/single cell necrosis was recorded in group 2 males. Following the recovery periods, the hepatocellular hypertrophy had undergone partial or complete resolution in group 2 and 5 males and in group 6 females. The incidence of pancreas apoptosis/single cell necrosis in group 2 males had also returned to the level of the vehicle control group. BACK TO MAIN PATHOLOGY REPORT Page 5/146 Test Article: telomer alcohol/telomer acrylate/, ../pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 M a t e r ia ls a n d M eth od s Study Design D ose gro u p i V eh icle con trol D ose mg/kg N um ber o f r a t s m ale s . m ain x e^c . l fe m a le s . m ain r e^c . l a 3/3 8 3/3 T elom er a lco h o l 30 8 3/3 8 3/3 Telom er a c ry la te 4 5 PFHS 6 PFOS 30 30 10 3 8 3/3 8 3/3 8 3/3 8 3/3 8 3/3 8 3/3 8 3/3 8 3/3 m ain 1 -8 A n im a l n u m b ers m ale s r e c .l ree. 2 Tnai n fe m a le s r e c .l rea. 2 9 -11 13 -15 97-104 10 5-10 7 1 0 9 -1 1 1 17-24 25-27 29-31 113-120 121-123 125-127 33-40 . 41-43 49-56 65-72 81-88 57-59 73-75 89-91 45-47 129-136 137-139 14 1-14 3 61-63 77-79 93-95 145-152 161-168 177-18 4 153-155 16 9 -17 1 18 5 -18 7 15 7 -15 9 1 7 3 --1 7 5 18 9 -19 1 Administration of the Test Article The test article was administered daily by gavage for 28 days. The recovery group rats were allowed a 14-day (recovery 1) or 28-day (recovery 2) treatment free period. Rats of the control group 1 received the vehicle, IS aqueous carboxymethyl cellulose, alone. Necropsy and Histopathology At the end of the assigned study periods, the rats were killed by exsanguination following anesthesia by ether vapour. Complete necropsies were performed on all rats. Representative tissue samples of the following organs were preserved in 4% phosphate buffered neutral formaldehyde solution (10% formalin). Adrenal glands, aorta, bone - sternum and femur including joint; bone marrow sternal, brain, clitoral glands, epididymides, esophagus, eyes with optic nerve and Harderian glands; heart, kidneys, lacrimal glands - exorbital, large intestine - cecum, colon and rectum; larynx, liver, lungs, lymph nodes mandibular and mesenteric; female mammary gland area, nasopharynx, ovaries, pancreas, pituitary gland, preputial glands, prostate gland, salivary glands mandibular and sublingual; sciatic nerve, seminal vesicles, skeletal muscle, skin, small intestine - duodenum, jejunum and ileum with Peyer's patches; spinal cord - cervical, midthoracic and lumbar; spleen, stomach, testes, thymus, thyroid glands with parathyroid glands, tongue, trachea, urinary bladder, uterus with uterine cervix, vagina and all organs or tissues with macroscopic abnormalities. BACK TO MAIN PATHOLOGY REPORT Page 6/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/peos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Materials and Methods . Following fixation, the following organs from all animals, were trimmed, processed and embedded in paraffin wax. Sections were cut at a thickness of 2-4 micrometers and stained with hematoxylin and eosin. Numbers of sections made are given in parentheses. Adrenal glands (2), kidneys (2), liver (2), lymph nodes - mandibular (2) and mesenteric (1), ovaries (2), pancreas (1), spleen (1), testes (2), thymus (1) and all organs or tissues with macroscopic findings. Initially sections of the above organs from all main study rats were examined by light microscopy. Following this examination, sections of liver from groups 1 (vehicle control), 2 (telomer alcohol), 5 (PFHS) and 6 (PFOS) males and groups 1 and 6 females recovery allocations 1 and 2 were examined. Sections of pancreas from group 1 and 2 males of recovery allocations 1 and 2 were also examined. The sections were in February/March, 1999. Data Compilation The animal data and macroscopic findings were electronically transferred from the necropsy raw data files of NOTOX into the computer system of Propath GmbH where the microscopic findings were recorded by the undersigned pathologist using on line input Macroscopic findings are presented in summary in the table "Incidence of Macroscopic Findings" and in full descriptive terms in the "Individual Animal Data Records". Wherever possible, macroscopic findings were correlated with a microscopic finding. Microscopic findings are listed for each animal along with severity grades in the table "Individual Microscopic Findings" and summarized in the tables "Incidence of All Microscopic Findings" and "Incidence Table - Selected Microscopic Findings with Grades". They are further given' in full descriptive terms in the "Individual Animal Data Records". Histologic changes were described according to their distribution, severity, and morphologic character. BACK TO MAIN PATHOLOGY REPORT Page 7/146 Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos NOTOX no. Test System : Exploratory 28-Day Gavage Study in Rats Frbpath no. Sponsor : 3M Toxicology Services DDaattee 242933 99010 21.Apr.1999 Results Mortality A female group 3 rat, no. 129, died at blood sampling on the day of scheduled sacrifice. All other rats survived their assigned study periods. . Macroscopic Findings The few macroscopic findings recorded at all sacrifices were unremarkable and did not distinguish treated rats from-the vehicle control group. Microscopic Findings Treatment related microscopic findings were noted in the liver and pancreas. At the end of the main study, midzonal/centrilobular hypertrophy was seen at a minimal or slight degree in six group 2 (telomer alcohol) males, minimal to moderate degrees in all group 5 (PFHS) males and all group 6 (PFOS) rats of both sexes. By the end of the 28-day recovery period (recovery 2)this finding had partially or completely resolved in group 2 and 5 males and in group 6 females, however remained in evidence in group 6 males. In the pancreas a slight increase in the incidence of minimal or slight degrees of exocrine apoptosis/single cell necrosis was recorded in group 2 males at the end of the main study. The incidence of this finding returned to the level of the vehicle control group following the recovery periods. The remainder of the microscopic findings recorded at both necropsies, were within the range of background pathology encountered in Sprague Dawley rats of this age and strain and occurred at similar incidences and severity in both control and treated rats. ' BACK TO MAIN PATHOLOGY REPORT Page 8/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Conclusions . Morphologic alterations following the administration of Telomer alcohol/Telomer acrylate/ /PFHS/PFOS by once daily gavage to Sprague Dawley rats for twenty eight days, were present in the liver of telomer alcohol, PFHS and PFOS treated rats and in the pancreas of telomer alcohol treated rats. Hepatocellular hypertrophy was present in group 2 (30 mg/kg/day telomer alcohol), group 5 (10 mg/kg/day PFHS) males and in both sexes of group 6 (3 mg/kg/day PFOS) at the end of the main study. In the pancreas a slight increase in the incidence of exocrine apoptosis/single cell necrosis was recorded in group 2 males. Following the recovery periods, the hepatocellular hypertrophy had undergone partial or complete resolution in group 2 and 5 males and in group 6 females. The incidence of pancreas apoptosis/single cell necrosis in group 2 males had also returned to the level of the vehicle control group. BACK TO MAIN PATHOLOGY REPORT Page 9/146 Test Article: telomer alcohol/telomer acrylate/' ,/pehs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Pr&path n o .: 99010 Date : 21.Apr.1999 Incidence Table -- Macroscopic Findings M ain S tu d y SEX: DOSE GROUP: num ber o f an im als Lungs H em orrhage Stom ach H em orrhage L ive r E n larg ed K id n evs P e lv ic d ila tio n T estes Reduced in s iz e E p id id ym id es Reduced in s iz e O v a rie s W atery c y s t U teru s C y st(s ) MALE 123 45 6 888888 00001 0 00001 0 00 001 0 000000 00001 0 00001 0 VEMAT.il! 123 4 5 6 888 8 8 8 000 0 0 0 000 0 0 0 000 0 0 0 01 00 0 0 1 000 0 0 000 1 0 0 BACK TO MAIN PATHOLOGY REPORT Page 10/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prpath no. Date 242933 99010 21.Apr.1999 Incidence Table -- Macroscopic Findings .Recovery Allocation 1 SEX: DOSE GROUP: num ber o f a n im als L ive r A ccen tu ated lo b u la r p attern T estes Reduced In s iz e S k in A lo p e c ia gen eral c h e s t re g io n Scab fo rm a tio n ab d o m in al re g io n M axs 1 2 3 45 6 3 3 3333 0 0 0 001 01 0 01 0 0 0 0 000 1 0 0000 0 0 0 000 JH2MU2 12345 6 33 3 3 3 3 00 000 0 01 000 0 000000 1 00000 BACK TO MAIN PATHOLOGY REPORT Page 11/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Incidence Table -- Macroscopic Findings JRecovexy Allocation 2 . SEX: DOSE GROUP: num ber o f an im als Eyes Reduced in s iz e le n s M A LS 1 23 4 S6 3 3 3 3 33 0 0 0 1 00 12 3 4 5 33 4 3 3 6 3 00000 0 BACK TO MAIN PATHOLOGX REPORT Page 12/146 Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Prtpath no.: 99010 Date : 21.Apr.1999 Incidence Table - Selected Microscopic Findings with Grades Main Study SEX: DOSE GROUP: num ber o f a n im als L ive r num ber exam ined M id zo n al/ c e n trilo b u la r h yp ertrop h y, d iffu se (m in im al) (slig h t) (m od erate) T o ta l P an creas num ber exam ined A p o p to sis/ sin g le c e ll n e c r o s is , exo crin e (m in im al) (slig h t) T o ta l M US 12 3 45 6 8 8 8888 888888 123 45 6 88888 8 88888 8 05 00 0 0 0 1 002 2 0 000 66 0 60088 0000 0 1 000 0 0 4 0000 0 3 0000 0 8 88 8888 8888 8 8 1 4 2 02 1 1 201 01 2 62 1 2 2 20001 0 000 00 0 2 00 01 0 BACK TO MAIN PATHOLOGY REPORT Page 13/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prbpath no. Date 242933 99010 21.A p r . 1999 Incidence Table - Selected Microscopic Findings with Grades Recovery Allocation 2 SEX: DOSE GROUP: num ber o f an im als L ive r MALS 1 2 '3 333 45 3. 3 6 3 12345 6 3 33 33 3 num ber exam ined M id zo n al/ c e n trilo b u la r h ypertroph y, d iffu s e (m in im al) (slig h t) (m oderate) T o ta l 33 01 00 00 01 33 3 10 11 02 23 0 0 0 0 3 1 0 0 1 Recovery Allocation 2 SEX: DOSE GROUP: num ber o f a n im als L ive r num ber exam ined M id zo n al/ c e n trilo b u la r h yp ertrop h y, d iffu s e (m in im al) (slig h t) (m od erate) T o ta l P a n creas num ber exam ined A p o p to sis/ sin g le c e ll n e c r o s is , exo crin e (m in im al) MALS 1 23 45 6 33 3333 33 33 WVMAr.T? 12 3 45 6 3333 3 3 33 01 00 00 01 33 10 01 01 01 03 0 0 0 0 1 0 0 1 BACK TO MAIN PATHOLOGY REPORT Page 14/146 Test Article: telomer alcohol/elomR acrylate/, ./pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Incidence Table - Microscopic Findings Main Study SEX: DOSE GROUP: number o f a n im als Lungs num ber exam ined I n tr a - a lv e o la r b lo o d , necropsy a rte fa c t V a scu la r m ine r a l i s a t io n , fo ca l Stom ach ' num ber exam ined L ive r num ber exam ined In flam m ato ry c e l l fo c i S in g le c e ll n e cro sis M id zo n al/ c e n trilo b u la r h ypertroph y, d iffu s e H e p a to ce llu la r v a cu o la tio n H em ato p o ietic c e l l fo ci C o a g u la tiv e n e c ro s is , fo ca l P an creas num ber exam ined A p o p to sis/ sin g le c e ll n e c r o s is , exo crin e E x o crin e atro p h y, fo ca l to u s 123 45 6 888888 1 1 1 1 888888 888888 6663 66 0 60088 10003 4 3 00001 000010 8 88888 2 62 122 0 1 1 00 0 wn.'jwffl T.TS 12 3 4 5 6 888 8 8 8 8 8 8 .8 8 8 88888 8 7 65 3 5 4 000 0 0 8 10101 0 00010 0 00000 0 88888 8 2000 1 0 00100 0 BACK TO MAIN PATHOLOGY REPORT Page 15/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Prbpath no.: 99010 Date : 21.Apr.1999 Incidence Table - Microscopic Findings SEX: DOSE GROUP: num ber o f an im als MALE 12 3 45 6 8 8 '8 8 8 8 P an creas In fla m m a tio n , m ixed g ra n u lo c y tic / lym p h o cytic 000010 K id n eys num ber exam ined T u b u lar a tro p h y, c o rtic a l In te rs titia l in fla m m atio n , lym p h o cytic H yalin e c a s t(s ) T u b u lar m in e ra liza tio n 8 8888 8 3 55 34 6 3 12 13 2 1 10 20 2 0 1 00 0 0 P e lv ic in fla m m atio n , lym p h oid 0 0 1 00 0 P e lv ic d ila tio n C y s tic tu b u le s P e lv ic m in e ra liza tio n T u b u lar e p it h e lia l h yp ertrop h y, fo c a l 000000 000011 010000 000000 T estes num ber exam ined S p e rm a tid ic g ia n t c e ll(s ) 888888 1 000 01 S em in ifero u s tu b u la r atrop h y 100010 ZEM3XJ5 12 3 4 5 6 88888 8 0 000 0 0 8 888 8 8 4 12105 002103 0000 0 0 10000 0 000 0 0 0 01000 0 00011 0 1000 00 0000 1 0 BACK TO MAIN PATHOLOGY REPORT Page 16/146 Test Article: telomer alcohol/ telomer acrylate/ . / pfh s/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prpath no. Date 242933 99010 21.Apr.1999 Incidence Table - Microscopic Findings SEX: DOSE GROUP: MALS 12 3 num ber o f an im als 8 8 '8 E p id id ym id es num ber exam ined A trop h y A sp erm ia ' O va ries num ber exam ined U teru s num ber exam ined C yst(s) A d re n a l G lan d s num ber exam ined 888 E x tr a c a p s u la r n od ule 000 V a cu o la tio n , m u ltifo c a l in z. fa s ic u la ta 000 A trop h y, c o r tic a l d iffu se 000 S p leen . num ber exam ined 888 H em o p o iesis, p rim a rily e ry th ro p o ie sis 888 H em osid erin pigm en t 67 5 Thvm us num ber exam ined 888 Lym phoid a tr o p h y in v o lu tio n .0 0 1 4S 88 1 1 1 88 11 00 00 88 88 74 8. 8 10 6 8 8 0 2 1 8 8 4 8 2 Eh42H*B 12 3 45 6 8888 8 8 88888 8 r l 88888 8 01 1 01 1 00000 0 000 00 0 88888 8 7 88 5 8 5 88888 8 888 113 88 11 8 1 BACK TO MAIN PATHOLOGX REPORT Page 17/146 Test Article: telomer alcohol/ telomer acrylate/ / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in: Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 In c id e n c e T a b le -- M ic r o s c o p ic F in d in g s SEX: DOSE GROUP: M&LB 1 23 45 6 nunfeer o f a n im a ls B 8 '8 8 8 8 Thym us L ym p h o cyto lysis, in cre a se d 001 1 00 M e s e n t e r ic Lymph Node num ber exam ined 8888 88 C o n gestio n / e ry th ro p h a g o cy to sis 01 01 00 M a n d ib u la r Lvraoh Node num ber exam ined 8888 88 P la sm a cy to sis 5 63242 Lym phoid h y p e r p la s ia 2001 00 C o n g estio n / e ry th ro p h a g o cy to sis 001 1 2 0 FEMALE i 2345 6 8888 8 8 01 1 1 1 0 888 8 8 8 00 0 0 0 0 8888 8 8 03 2 1 1 2 11 23 1 0 0000 0 0 BACK TO MAIN PATHOLOGY REPORT Page 18/146 Test Article: telomer alcohol/T elomer acrylate/ . / pfhs/ pfos NOTOX no. : 242933 Test System : Exploratory 28-Day Gavage Study in Rats ' Propath no.: 99010 Sponsor : 3M Toxicology Services Date : 21.Apr.1999 Incidence Table - Microscopic Findings Recovery Allocation 1 SEX: DOSE GROUP: num ber o f a n im als L ive r num ber exam ined In flam m ato ry c e l l fo c i S in g le c e ll n e c ro sis M id zo n al/ c e n trilo b u la r h yp ertrop h y, d iffu s e H e p a to ce llu la r v a cu o la tio n H em ato p o ietic c e l l fo c i MALS 1 2 '3 4 5 6 - 3 3 3' 3 3 3 33 33 23 01 33 33 32 23 10 01 03 00 P a n creas num ber exam ined E x o crin e atro p h y, fo ca l T estes num ber exam ined Sem iniferous tu b u lar atrop h y S k in num ber exam ined In flam m atio n , e x u d a tiv e 33 01 1 1 1 1 T.TC 1 2345 6 3333 3 3 33 33 21 01 10 00 11 10 BACK TO MAIN PATHOLOGY REPORT Page 19/146 Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /', , / p f h s / p f o s Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prbpth no. Date 242933 99010 21.Apr.1999 Incidence Table - Microscopie Findings Recovery Allocation 2 SEX: DOSE GROUP: num ber o f a n im als L ive r num ber exam ined In flam m ato ry c e l l fo ci S in g le c e ll n e c ro sis M id zo n al/ c e n trilo b u la r h yp ertrop h y, d iffu s e H e p a to ce llu la r v a cu o la tio n C o a g u la tiv e n e c r o s is , fo ca l . P a n creas mis 1 23456 3 3 33 33 33 33 22 01 33 33 22 03 10 01 11 00 num ber exam ined A p o p to sis/ sin g le c e ll n e c r o s is , ex o crin e E yes num ber exam ined P e rio rb ita l in fla m m atio n due to b lo o d sam p lin g 33 10 1 1 FEMAU2 12345 6 33 3 3 3 3 33 33 12 01 00 0. 0 BACK TO MAIN PATHOLOGX REPORT Page 20/146 Test Article: telomer alcohol/ telomer acrylate/ ,/ pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. 242933 Prbpath no. ' 99010 Date 21.Apr.1999 Individual Animal Microscopic Findings Codes and symbols in table heading: m Males f Females Codes and symbols in animal lines: p Planned sacrifice (main study) r Recovery 1 (plannedsacrifice) s Recovery 2 (plannedsacrifice) o Other Grading system used in finding lines: 0 finding not present 1 minimal 2 slight 3 moderate 4 severe 5 very severe x present Only organs/groups with findings are listed in the table BACK TO MAIN PATHOLOGY REPORT Page 21/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Ptpath no. Date 242933 ' 99010 21.A p r . 1999 Individual Animal Microscopia Findings Hales, Dose group 1 12345 67 8 m .m m m m m m m necropsy status Liver PPPPP PPP Inflammatory cell foci 22221122 Single cell necrosis Hepatocellular vacuolation 01111 12 0 00000 010 Hematopoietic cell foci 1 010 0 0 1 0 Pancreas Apoptosis/single cell necrosis, 0 0 1 0 2 0 0 0 exocrine Kidneys Ttabular atrophy, cortical 0001010 1 Interstitial inflammation, lymphocytic 0000110 1 Hyaline cast(s) 000000 10 Testes Spermatid!c giant cell(s) 0.1 0 0 0 0 0 0 Seminiferous tubular atrophy 0 1000000 Spleen Hemopoiesis, primarily erythropoiesis 2 2211 12 1 Hemosiderin pigment 0 1112210 Mandibular Lymph Node Plasmacytosis 2 2 22 03 0 0 Lymphoid hyperplasia 02200000 BACK TO MAIN PATHOLOGX REPORT Page 22/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ . ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prpath no. Date 242933 99010 21.A p r . 1999 Individual Animal Microscopic Findings Hales, Bose group 1 necropsy status Liver 9 10 11 13 14 IS m .m m m m m r r rsss Inflammatory cell foci Single cell necrosis Hepatocellular vacuolation Pancreas 2 32222 0 2 1110 0 0 1100 Apoptosis/single cell necrosis, 0 0 0 0 0 1 exocrine BACK TO MAIN PATHOLOGY REPORT Page 23/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Pr'opath no. Date 242933 99010 21.Apr.1999 Individual Animal Microscopie Finding's Males, Dosa group 2 necropsy status Liver 17 18 19 20 21 22 23 24 m .m m m m m m m P P P P P P P .P Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse 1 2 122 2 2 2 1 00 11 1 1 1 0 11 112 0 1 Pancreas Apoptosis/single cell necrosis, 2 1 0 1 0 2 1 1 exocrine Exocrine atrophy, focal Kidneys 0 00 00 0 0 1 Tubular atrophy, cortical Interstitial inflammation, lymphocytic 2 1011100 0 00 01 0 0 0 Hyaline cast(s) Tubular mineralization Pelvic mineralization S p le e n 0 0 0 01 0 0 0 0 100000 0 01000000 Hemopoiesis, primarily erythropoiesis 1 11112 12 Hemosiderin pigment Mesenteric Lymph Node 0 1 121111 Congestion/erythrophagocytosis 0 0 1 0 0 0 0 0 Mandibular Lymph Node Plasmacytosis 2 32 02022 BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 24/146 NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Microscopic Findings Hales, Dose group 2 necropsy status 25 26 27 29 30 31 m .m m m m m r rr s s s liver Inflammatory cell foci 2 332 2 2 Single cell necrosis 1 110 1 1 Midzonal/centrilobular hypertrophy, diffuse 0 101 0 0 Hematopoietic cell foci 1 000 0 0 Coagulative necrosis, focal 0 000 1 0 Pancreas Exocrine atrophy, focal Testes 0 0100 0 Seminiferous tubular atrophy 4 BACK TO MAIN PATHOLOGY REPORT Page 25/146 Test Article: telomer alcohol/ telomer acrylate/ ./ pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Microscopic Findings Males, Dose group 3 necropsy status Liver 33 34 35 36 37 38 39 40 m .m m m m m a m P PPPPP PP Inflammatory cell foci Single cell necrosis Pancreas 2 1123222 1 10 11 1 1 0 Apoptosis/single cell necrosis, 1 0 0 0 0 0 1 0 exocrine Exocrine atrophy, focal Kidneys 0 000001 0 Tubular atrophy, cortical Interstitial inflammation, lymphocytic 1. 0 0 1 1 0 1 1 0 101000 0 Pelvic inflammation, lymphoid 0 00000 1 0 Spleen Hemopoiesis, primarily erythropoiesis 2 11122 2 1 Hemosiderin pigment Thymus 1 0001111 Lymphoid atrophy - involution Lymphocytolysis, increased Mandibular Lymph Node 0 0000100 0 0000002 Plasmacytosis 0 2 0 02 2 0 0 Congestion/erythrophagocytosis 0 0 0 0 0 0 0 3 BACK TO MAIN PATHOLOGY REPORT Page 26/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 2 1 .Apr.1999 Individual Animal Microscopic Findings Males, Bose group 4 necropsy status Liver 49 so 51 52 53 54 55 56 m . m m m m m mi a PPPPPPPP Inflammatory cell foci 2 111222 1 Single cell necrosis 1 10 0 0 X 0 0 Pancreas Apoptosis/single cell necrosis, 0 0 0 0 0 0 2 0 exocrine Kidneys Tubular atrophy, cortical Interstitial inflammation, lymphocytic 1000X001 0000X000 Hyaline cast(s) 00000101 Adrenal Glands Extracapsular nodule Spleen 0 000000X Hemopoiesis, primarily erythropoiesis Hemosiderin pigment Thymus X 12 12 X2 X 011XXXXX Lymphoid atrophy - involution Lymphocytolysis, increased Mesenteric Lymph Node 0000000X 0 0 0 0 0 0 0. 2 Congestion/erythrophagocytosis Mandibular Lymph Node 00010 000 Plasmacytosis 0 0 02 0 02 0 Lymphoid hyperplasia 0 0 02 0 00 0 Congestion/erythrophagocytosis 0 0 0 2 0 0 0 0 BACK TO MAIN PATHOLOGY REPORT Page 27/146 Test Article: telomer alcohol/ telcmer acrylate/ . / pfh s/ pfo s Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21 Apr.1999 Individual Animal Microscopic Findings Males, Dose group 4 necropsy status 5 7 58 59 6 1 62 63 m .m m m m m r rr s s s Eyes Periorbital inflammation due to blood sampling 3 BACK TO MAIN PATHOLOGY REPORT Page 28/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/' /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Microscopic Findings Males, Dosa group 5 necropsy status Lungs Intra-alveolar blood, necropsy artefact 65 66 67 68 69 70 71 Ta m .m m m m m m m P P P PPP P P X Vascular mineralisation, focal 1 Liver Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse Hepatocellular vacuolation Coagulative necrosis, focal Pancreas 2 1 222222 10101 111 3 33332 2 3 2 0 1000 0 1 0 0 1000 0 0 Apoptosis/single cell necrosis, 0 1 0 1 0 0 0 0 exocrine Inflammation, mixed granulocytic/lymphocytic 0 0 00010 0 Kidneys . Tubular atrophy, cortical Interstitial inflammation, lymphocytic 1 1 11 0 0 0 0 0 0 0 1110 0 Cystic tubules Testes Seminiferous tubular atrophy Epididymides 0 0 00002 0 0 4000000 ' Atrophy Aspermia 3 X BACK TO MAIN PATHOLOGY REPORT Page 29/146 Test Article: telomer alcohol/ telomr acrylate/ / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Pfopath no.: 99010 Date : 21.Apr.1999 Individual Animal Microscopio Findings Males, Dose group 5 necropsy status Adrenal Glands 65 66 67 68 69 70 71 72 m .m m m m m m m PPPPPPPP Extracapsular nodule 0000X 000 Spleen Hemopoiesis, primarily erythropoiesis Hemosiderin pigment ' 2 12 1 i2 1 2 0 102 0 0 12 Mandibular Lymph Node Plasmacytosis 03200022 Congestion/erythrophagocytosis 0 1 0 0 0 0 0 2 BACK TO MAIN PATHOLOGY REPORT Page 30/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 2 1 .Apr.1999 Individual Animal Microscopia Findings Hales, Vose gxoup 5 necropsy status liver 73 74 75 77 78 79 m .m m m m m rrrsss Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse 3 22222 2 11110 201000 Hepatocellular vacuolation Testes 0 0000 1 Seminiferous tubular atrophy 3 BACK TO MAIN PATHOLOGY REPORT Page 31/146 Test Article: t e l o m e r a l c o h o l / t e l c m e r a c r y l a t e / / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21 Apr.1999 Individual Animal Microscopic Findings Hales, Lose group 6 necropsy status Liver 81 82 83 84 85 86 87 88 m mmmmmm m p P P PPP P P In fla m m a to ry c e l l f o c i Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse 2 2 22 12 2 2 1 0 1101 1 1 2333323 3 Hepatocellular vacuolation Hematopoietic cell foci Pancreas 0110012 0 1 0 0000 0 0 Apoptosis/single cell necrosis, 0 2 0 0 0 0 0 1 . exocrine Kidneys Tubular atrophy, cortical 1101111 0 Interstitial inflammation, lymphocytic 0 021000 0 Hyaline cast(s) Cystic tubules T estes 0 0 0001 1 0 0 020000 0 Spermatidic giant cell(s) Adrenal Glands 0 010000 0 Vacuolation, multifocal in z. fasiculata 0 0 00010 1 Atrophy, cortical diffuse 0 0 02000 0 BACK TO MAIN PATHOLOGY REPORT Page 32/146 Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e / / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prbpath no. Date 242933 99010 2 1 .Apr.1999 Individual Animal Microscopic Findings MAZes, Dose group 6 necropsy status Spleen . 81 82 83 84 85 86 87 m .m m m m m m PPPPPPP Hemopoiesis, primarily erythropoiesis 1 1 1 112 1 Hemosiderin pigment 1110000 Thymus Lymphoid atrophy - involution 00000201 .Mandibular Lymph Node . Plasmacytosis 30020000 88 m P 1 1 BACK TO MAIN PATHOLOGY REPORT Page 33/146 Test Article: t e l o m e r a l c o h o l / e l o m r a c r y l a t e / ,'pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 I n d iv id u a l A n im a l M ic r o s c o p ic F in d in g s Malas, Dosa group 6 necropsy status 89 90 91 93 94 95 m mmmm m r r r `s 3 3 Liver Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse 2 22i2 2 1 10i0 1 2 33i32 Hepatocellular vacuolation 1 22010 BACK TO MAIN PATHOLOGY REPORT Page 34/146 Teat Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e / / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal Microscopic Findings Females, Dose group 1 necropsy status Liver 97 98 99 100 101 102 103 104 f .f f f f f f f P P Pp p PP P Inflammatory cell foci 2 121222 1 Single cell necrosis 1 0 111 11 1 Hepatocellular vacuolation 0 000000 1 Pancreas Apoptosis/single ceil necrosis, 0 1 1 0 0 0 0 0 exocrine Kidneys Tubular atrophy, cortical Tubular mineralization Pelvic mineralization Spleen 10010101 0 0 00 100 0 0 100000 0 Hemopoiesis, primarily erythropoiesis 11110111 Hemosiderin pigment Thymus Lymphoid atrophy - involution Mandibular Lymph Node 3 2223322 0 0000010 Lymphoid hyperplasia 0 0002000 BACK TO MAIN PATHOLOGY REPORT Page 35/146 Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e / /pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Microscopic Findings Females, Dose group 1 necropsy status 105 106 107 109 110 111 f .f f f f f r rrss s Liver Inflammatory cell foci 22222 1 Single cell necrosis Hepatocellular vacuolation 110100 020000 Skin Inflammation, exudative 2 BACK TO MAIN PATHOLOGY REPORT Page 36/146 Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e / ,/pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prbpath, no. Date 242933 99010 21.A p r . 1999 Individual Animal Microscopic Findings Females/ Dosa group 2 necropsy status Liver 113 114 115 116 117 118 119 120 f . f f f f f f f P PPPPP P P Inflammatory cell foci Single cell necrosis 2 2111112 1 101011 1 Kidneys Tubular atrophy, cortical 0 000001 0 Pelvic dilation 0 000X 0 0 0 Adrenal Glands Extracapsular nodule 0 0 00X 0 0 0 Spleen Hemopoiesis, primarily erythropoiesis 1111i11 1 Hemosiderin pigment 2222223 2 Thymus Lymphoid atrophy - involution 0000010 0 Lymphocytolysis, increased M a n d ib u la r Lymph N ode 0000001 0 Plasmacytosis 2 2 00030 0 Lymphoid hyperplasia 2 000000 0 BACK TO MAIN PATHOLOGY REPORT Page 37/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Microscopic Findings Females, Dose group 3 necropsy status Liver 129 130 131 132 133 134 135 136 f .f f f f f f f oP P PPP P P Inflammatory cell foci Single cell necrosis Hepatocellular vacuolation Pancreas Exocrine atrophy, focal Kidneys Tubular atrophy, cortical Interstitial inflammation, lymphocytic Adrenal Glands 22 1121 12 1 1 102 0 0 1 0 1 000 0 0 0 0 0 0100 0 0 01000100 0000010 1 Extracapsular nodule Spleen 0 000000X Hemopoiesis, primarily erythropoiesis 2 1 11211 i Hemosiderin pigment Thymus Lymphoid atrophy - involution Lymphocytolysis, increased Mandibular Lymph Node 1 2 2 12 22 3 0 100100 1 0 0 00010 0 Plasmacytosis Lymphoid hyperplasia 0 0000022 0 0000022 BACK TO MAIN PATHOLOGY REPORT Page 38/146 Test Article: telomer alcohol/ telomer acrylate/ / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propth no. Date 242933 99010 21.Apr.1999 Individual Animal Microscopic Findings Females, Dose group 4 necropsy status Liver 145 146 147 148 149 150 151 152 f . f f f f f f f P PPPPPPP Inflammatory cell foci Single cell necrosis Hematopoietic cell foci Kidneys 1 2 1 2 2 2 2 1 0 1100 0 0 1 0 0000 100 Tubular atrophy, cortical Interstitial inflammation, lymphocytic 0 0000001 0 0010000 Cystic tubules Uterus 0 0200 0 0 0 Cyst(a) Spleen X Hemopoiesis, primarily erythropoiesis 1 10110 0 1 Hemosiderin pigment Thymus 2 22223 2 2 Lymphoid atrophy - involution Lymphocytolysis, increased Mandibular Lymph Node 0 0000001 0 0010000 Plasmacytosis Lymphoid hyperplasia 0 0000200 2 0200200 BACK TO MAIN PATHOLOGY REPORT Page 39/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prpath no.' Date 242933 99010 21.Apr.1999 Individual Animal Microscopic Findings Females, Dose group 5 ' necropsy status Liver Inflammatory cell foci Single cell necrosis 161 162 163 164 165 166 167 168 f fffff f f p PPPP P P P 223211 1 1 011010 1 1 Hepatocellular vacuolation 000000 0 1 Pancreas Apoptosis/single cell necrosis, 0 0 0 0 . 1 0 0 0 exocrine Kidneys Cystic tubules 0 00000 0 1 Tubular epithelial hypertrophy, focal 1 00000 0 0 Adrenal Glands Extracapsular nodule Spleen 0 0000X 0 0 Hemopoiesis, primarily erythropoiesis H em o sid erin p ig m en t Thymus 112111 1 1 2 22222 2 3 Lymphoid atrophy - involution 0 00200 0 0 Lymphocytolysis, increased 0 0 1000 0 0 Mandibular Lymph Node Plasmacytosis Lymphoid hyperplasia 0 020000 0 0000020 0 BACK TO MAIN PATHOLOGY REPORT Page 40/146 Test Article: t e l o m e r a l c o h o l / t e l c m e r a c r y l a t e / / p f h s / p f o s Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 ` 99010 21.A p r . 1999 Individual Animal Microscopic Findings Females, Dose group necropsy status Liver 177 178 179 180 181 182 183 184 f .f f f f f f f P P P P P P 'P P Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse 2 1221111 10101010 22312 332 Kidneys Tubular atrophy, cortical 1 1001 1 0 1 Interstitial inflammation, lymphocytic 01010010 Adrenal Glands Extracapsular nodule X 0000000 Spleen Hemopoiesis, primarily erythropoiesis i 0 011 1 0 1 Hemosiderin pigment 2 132322 2 Thymus Lymphoid atrophy - involution 10000000 Mandibular Lymph Node Plasmacytosis 0 0 00022 0 BACK TO MAIN PATHOLOGY REPORT Page 41/146 Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e / / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010' 21.Apr.1999 Individual Animal Microscopic Findings females, Dose group 6 necropsy status 185 186 187 189 190 191 f .f f f f f r rr3ss Liver Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse 1 12211 0 0 1 1 1' 0 0 10001 BACK TO MAIN PATHOLOGY REPORT Page 42/146 Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e / / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Animal Data List Males ANIMAL SEX FINAL TEST FIRST LAST NUMBER M/F STATE DATS DAT . DAT DATE NECROPSY DOSE GROUP 1 1 zn p 2m p 3m p 4m p . 5m p 6m p 7m p 8m p 9m r 10 m r 11 m r 13 m 3 14 za 3 15 a 3 DOSE GROUP 2 17 m P 18 m P 19 za P 20 za P 21 za P 22 za P 23 in P 24 m P 25 za r 26 za r 27 za r 29 m 3 30 za 3 31 za 3 DOSE GROUP 3 33 za P 34 za P 35 in P 36 za P 37 za P 38 zn P 39 za P 40 za P 41 za r 42 za r 43 zn r 45 za 3 46 zn 3 47 za 3 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 BACK TO MAIN PATHOLOGY BEPORT Page 43/146 Test Article: telomer alcohol/ telomer acrylate/ ./pfhs/ pfos NOTOX no. : 242933 Test System : Exploratory 28-Day Gavage Study in Rats Propath no.: 99010 Sponsor : 3M Toxicology Services Date : 21.Apr.1999 A n im a l D a ta L i s t Males :mal SEX FINAL TEST FIRST LAST EER M/F STATE DAYS DAY . DAY DATE NECROPSY DOSS GROUP 4 49 m P SO m P 51 m P 52 m P S3 m P 54 a P 55 m P 56 m P 57 m r 58 m r 59 m r 61 m s 62 m s 63 m s DOSS GROUP 5C 65 m P 66 za P 67 m P 68 m P 69 m P 70 zn P 71 xa P 72 m P 73 m r 74 m r 75 za r 77 za s 78 za s 79 za s DOSE GROUP CO6 81 za P 82 za P 83 za P 84 za P 85 za P 86 za P 87 za P 88 za P 89 za r 90 za r 91 za r 93 za s 94 za s 95 za s 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98. 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 BACK TO MAIN PATHOLOGY REPORT Page 44/146 Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e / ./p f h s / pf o s Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : Propath no.: Date : 242933 99010 21.Apr.1999 Animal Data List F e m a le s ANIMAL SEX FINAL TEST FIRST NUMBER M/F STATE DAYS DAY . LAST DAY DATE NECROPSY DOSE GROUP 1 97 f 98 99 f 100 f 101 f 102 f 103 104 f 105 106 f 107 r 109 110 111 DOSE GROUP 2 113 114 115 116 117 118 119 120 121 122 123 125 126 127 DOSE GROUP 3 129 130 131 132 f 133 f 134 . 135 136 137 138 139 f 141 142 143 P P P P P P P P r E r s s s P P P P P P P P r r r s s s P P P P P P P r r r 3 s 3 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 BACK TO MAIN PATHOLOGY REPORT Page 45/146 Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e / / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Animal Data List females ANIMAL SEX FINAL TEST FIRST NUMBER M/F STATE DAYS DAY LAST DAY DATE NECROPSY DOSE GROUP 4 145 f P 146 P 147 f P 148 f P 149 f P 150 f P 151 f P 152 f P . 153 r 154 f r 155 f r 157 s 158 s 159 f s DOSE GROUP 5 161 f P 162 P 163 f P 164 P 165 P 166 f P 167 f P 168 f P 169 f r 170 f r 171 r 173 3 ' 174 s 175 s DOSE GROUP 6 177 P 178 f P 179 P 180 P 181 f P 182 P 183 P 184 f P 185 r 186 r 187 r 189 f 3 190 s 191 3 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98. 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 BACK TO MAIN PATHOLOGY REPORT Page 46/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 1 Male Vehicle Control MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Animal No: 2 M ACROSCOPIC FINDINGS days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy :03/11/98 No findings noted Inflammatory cell foci (slight) Hematopoietic cell foci (minimal) Hemopoiesis, primarily erythropoiesis (slight) Plasmacytosis (slight) Plasmacytosis (slight) No abnormalities found din: Pancreas, Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 47/146 Test Article: t e lo m e r a l c o h o l / te l o m e r a c r y l a t e / ' pehs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 2 Male Vehicle Control MICROSCOPIC FINDINGS Liver Testes/1 Testes/2 Spleen Mandibular Lymph Node/ 1 Animal No: 3 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Spleen Mandibular Lymph Node/ 1 Inflammatory cell foci (slight) Single cell necrosis (minimal) Seminiferous tubular atrophy (minimal) Spermatidic giant cell(s) (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) Plasmacytosis (slight) Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy :03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hematopoietic cell foci (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) Plasmacytosis (slight) Lymphoid hyperplasia (slight) BACK TO MAIN PATHOLOGY REPORT Page 48/146 Teat Article: telomer alcohol/telomer acrylate/ 'pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 3 Male Vehicle Control MICROSCOPIC FINDINGS (continued) Mandibular Lymph Node/ 2 Animal No: 4 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Mandibular Lymph Node/ 1 Plasmacytosis (slight) No abnormalities found in: Kidney/1, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminiti date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 49/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Frbpath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 5 M ale V e h ic le C o n tro l MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 Spleen Animal No: 6 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :04/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Apoptosis/single cell necrosis, exocrine (slight) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :04/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 50/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 ' 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 6 Male Vehicle Control MICROSCOPIC FINDINGS Liver Kidney/1 Kidney/2 Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Animal No: 7 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Inflammatory cell foci (minimal) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Plasmacytosis (moderate) Plasmacytosis (moderate) No abnormalities found in: Pancreas, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (slight) Hepatocellular vacuolation (minimal) Hematopoietic cell foci (minimal) Hyaline cast(s) (minimal) BACK TO MAIN PATHOLOGY REPORT Page 51/146 Test Article: telomer alcohol/telomer acrylate/ 'pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 9901 21.A p r . 1999 Individual Animal Data Records Animal Mo: SEX: DOSE GROUP: 7 Male V e h ic le C o n tro l MICROSCOPIC FINDINGS (continued) Spleen Animal No: 8 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver KLdney/1 Kidney/2 Spleen Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :04/11/98 No findings noted Inflammatory cell foci (slight) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimal) No abnormalities found in: Pancreas, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN FATHOLOGX REPORT Page 52/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no. : 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 9 Male Vehicle Control MACROSCOPIC FINDINGS Skin MICROSCOPIC FINDINGS Liver Animal No: 10 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver . days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 chest region: Alopecia No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) No abnormalities found in: Pancreas. days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 No findings noted Inflammatory cell foci (moderate) Single cell necrosis (slight) No abnormalities found in: Pancreas. BACK TO MAIN PATHOLOGE REPORT Page 53/146 Test Article: telomer alcohol/t'elomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prbpath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 11 Male Vehicle Control MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 13 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver . days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) No abnormalities found in: Pancreas. days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) No abnormalities found in: Pancreas. BACK TO MAIN PATHOLOGY REPORT Page 54/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 14 Male Vehicle Control MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: IS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas days of treatment :29 sacrifice group :recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :02/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) No abnormalities found in: Pancreas. days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Apoptosis/single cell necrosis, exocrine (minimal) BACK TO MAIN PATHOLOGY REPORT Page 55/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ 7PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 97 F em ale V e h ic le C o n tro l M ACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen . Animal No: 98 . MACROSCOPIC FINDINGS Ovaries days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) No abnormalities found in: Pancreas, Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. , days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy :05/11/98 left side Watery cyst No abnormalities were found in any of the other tissues examined BACK TO MAIN PATHOLOGY REPORT Page 56/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no. : 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 98 Female Vehicle Control MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 Ovary/1 Spleen Animal No: 99 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Spleen Inflammatory cell foci (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Pelvic mineralization (minimal) No micro correlate Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Kidney/2, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status ;planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) ' No abnormalities found in: Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 57/146 Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 100 Female Vehicle Control MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Animal No: 101 MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) ' Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 . No findings noted BACK TO MAIN PATHOLOGY REPORT Page 58/146 Test Article: telomer alcohol/t'elomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no.: 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 101 Female Vehicle Control MICROSCOPIC FINDINGS Liver K id n ey /1 S p le e n M a n d ib u la r Lym ph N ode/ 1 Animal No: 102 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Inflammatory cell foci (slight) Single cell necrosis (minimal) Tubular mineralization (minimal) Hemosiderin pigment (moderate) Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy :06/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 59/146 Test Article: telomer alcohol/telcmer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prpath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 103 F em ale V e h ic le C o n tro l MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Thymus Animal No: 104 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid atrophy - involution (minimal) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 60/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal Ho: SEX: DOSE GROUP: 104 Female Vehicle Control MICROSCOPIC FINDINGS Liver Kidney/1 Spleen . Animal No: 105 MACROSCOPIC FINDINGS Skin MICROSCOPIC FINDINGS Liver Skin . Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 abdominal region: Scab formation No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Single cell necrosis (minimal) Inflammation, exudative (slight) (correlates to GROSS finding) BACK TO MAIN PATHOLOGY REPORT Page 61/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal Ho: SEX: DOSE GROUP: 106 Female Vehicle Control MACROSCOPIC FINDINGS . MICROSCOPIC FINDINGS Liver Animal No: 107 MACROSCOPIC FINDINGS ( ' MICROSCOPIC FINDINGS Liver Animal No: 109 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (slight) days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted Inflammatory cell foci (slight) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 BACK TO MAIN PATHOLOGY REPORT Page 62/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prbpath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 109 Female Vehicle Control MACROSCOPIC FINDINGS {continued) MICROSCOPIC FINDINGS Liver Animal No: 110 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 111 MACROSCOPIC FINDINGS . MICROSCOPIC FINDINGS Liver No findings noted . Inflammatory cell foci (slight) Single cell necrosis (minimal) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted Inflammatory cell foci (slight) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted Inflammatory cell foci (minimal) BACK TO MAIN PATHOLOGY REPORT Page 63/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal Ko: SEX: DOSE GROUP: 17 Male Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 KLdney/2 Spleen Mandibular Lymph Node/ 1 . Animal No: 18 MACROSCOPIC FINDINGS days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy :03/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Apoptosis/single cell necrosis, exocrine (slight) Tubular atrophy, cortical (slight) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Plasmacytosis (slight) No abnormalities found in: Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric lymph Node, Mandibular Lymph Node/2. days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 . date of necropsy :03/11/98 No findings noted . BACK TO MAIN PATHOLOGY REPORT Page 64/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS ' NOTOX no. Test System : Exploratory 28-Day Gavage Study in Rats Prpath no. Sponsor : 3M Toxicology Services Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 18 Male Telomer Alcohol MICROSCOPIC FINDINGS liver Pancreas Kidney/1 Kidney/2 Spleen Mandibular Lymph Node/ 1 . Animal No: 19 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Tubular atrophy, cortical (minimal) Pelvic mineralization (minimal) Tubular mineralization (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Plasmacytosis (moderate) No abnormalities found in: Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy :03/11/98 No findings noted Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) BACK TO MAIN PATHOLOGY REPORT Page 65/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 19 Hale Telamer Alcohol MICROSCOPIC FINDINGS (continued) Mesenteric lymph Node Mandibular Lymph Node/ 1 Animal No: 20 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 Spleen Congestion/erythrophagocytosis (minimal) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. r BACK TO MAIN PATHOLOGY REPORT Page 66/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 2 1 .Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 21 Hale Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Kidney/2 Spleen Mandibular Lymph Node/ 1 Animal No: 22 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) Interstitial inflammation, lymphocytic (minimal) Hyaline cast(s) (minimal) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Plasmacytosis (slight) ' No abnormalities found in: Pancreas, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. . days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 BACK TO MAIN PATHOLOGY REPORT Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Sponsor : 3M Toxicology Services Page 67/146 NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 22 Male Telomer Alcohol MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 Kidney/2 Spleen . Animal No: 23 MACROSCOPIC FINDINGS No findings ; Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Apoptosis/single cell necrosis, exocrine (slight) Tubular atrophy, cortical (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) No abnormalities found in: Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 68/146 Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prpath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: COSE GROUP: 23 Hale Telomer Alcohol MICROSCOPIC FINDINGS liver Pancreas Spleen Mandibular Lymph Node/ 1 Animal No: 24 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS liver Pancreas Spleen Inflammatory cell foci (slight) Single cell necrosis (minimal) ' Apoptosis/single cell necrosis, exocrine (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Plasmacytosis (slight) No abnormalities found in: Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) . Apoptosis/single cell necrosis, exocrine (minimal) Exocrine atrophy, focal (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) BACK TO MAIN PATHOLOGY REPORT Page 69/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 ' 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 24 . Hale Telomer Alcohol MICROSCOPIC FINDINGS (continued) Mandibular Lymph Node/ 1 ' Animal No: 25 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 26 MACROSCOPIC FINDINGS .. ' Plasmacytosis (slight) No abnormalities found in: Kldney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. . days of treatment : 29 . sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hematopoietic cell foci (minimal) No a b n o r m a litie s fo u n d in : P a n c re a s. days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/1/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 70/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath n o .: 99010' Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 26 Hale Telomer Alcohol MICROSCOPIC FINDINGS Liver Animal No: 27 MACROSCOPIC FINDINGS . Testes MICROSCOPIC FINDINGS Liver Pancreas Testes/l Testes/2 . Inflammatory cell foci (moderate) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) No abnormalities found in: Pancreas. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 Reduced in size FLACCID No abnormalities were found in any of the other tissues examined Inflammatory cell foci (moderate) Single cell necrosis (minimal) Exocrine atrophy, focal (minimal) Seminiferous tubular atrophy (severe) (correlates to GROSS finding) Seminiferous tubular atrophy (severe) (correlates to GROSS finding) BACK TO MAIN PATHOLOGY REPORT Page 71/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prbpath. no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 29 Male Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 30 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver ' . days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (minimal) No abnormalities found in: Pancreas. days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Coagulative necrosis, focal (minimal) No abnormalities found in: Pancreas. BACK TO MAIN PATHOLOGY REPORT Page 72/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ~ f'PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 31 Male Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: SEX: 113 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) No abnormalities found in: Pancreas. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Plasmacytosis (slight) Lymphoid hyperplasia (slight) BACK TO MAIN PATHOLOGY REPORT Page 73/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PEOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 113 Female Telamer Alcohol MICROSCOPIC FINDINGS (continued) Animal No: 114 MACROSCOPIC FINDINGS ' MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1' - No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 74/146 Test Article: telomer alcohol/telomer acrylate/ ./pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Pr'opath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 115 Female Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS liver Spleen Animal No: 116 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, KLdney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :28 . sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy :05/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) BACK TO MAIN PATHOLOGY REPORT Page 75/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System ; Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 116 Female Telomer Alcohol MICROSCOPIC FINDINGS (continued) Animal No: 117 MACROSCOPIC FINDINGS Kidneys MICROSCOPIC FINDINGS Liver Kidney/2 Adrenal Gland/1 Spleen . . - No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. . days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 right side Pelvic dilation No abnormalities vere found in any of the other tissues examined Inflammatory cell foci (minimal) Pelvic dilation present (correlatesto GROSS finding) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, ' Mandibular Lymph Node/2.. . BACK TO MAIN PATHOLOGY DEPORT Page 76/146 Test Article: telcmer alcohol/telomer acrylate/ ,/pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Pr'opath no.: 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 118 Female Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Thymus Mandibular Lymph Node/ 1 Animal No: 119 MACROSCOPIC FINDINGS days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date-of necropsy :06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid atrophy - involution (minimal) Plasmacytosis (moderate) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 77/146 Test Article: telomer alcohol/telomer acrylate/.. /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 119 Female Telomer Alcohol MICROSCOPIC FINDINGS Liver Kidney/2 Spleen Thymus Animal No: 120 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Inflammatory cell foci (minimal) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) Lymphocytolysis, increased (minimal) No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2 Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 78/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. PYopath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 121 Female Telomer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 122 MACROSCOPIC FINDINGS Skin MICROSCOPIC FINDINGS Skin Animal No: 123 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy :20/11/98 Alopecia No abnormalities were found in any of the other tissues examined No micro correlate days of treatment : 29 sacrifice group : recovery group necropsy status :planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 BACK TO MAIN PATHOLOGY REPORT Page 79/146 Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 123 Female Telomer Alcohol MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Animal No: 125 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 126 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT T e s t A r t i c l e : telomer alcohol/Telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 80/146 NOTOX no. Prbpath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 127 Female Telamer Alcohol MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: SEX: DOSE GROUP: 33 Male Telomer Acrylat MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 Kidney/2 Spleen days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Tubular atrophy, cortical (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) BACK TO MAIN PATHOLOGY REPORT T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 81/146 NOTOX no. : Prbpath no. : Date : 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 33 M ale T elo m er A c ry la t M ICROSCOPIC FINDINGS (co n tin u e d ) Animal No: 34 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Number of Sections less than protocol for Mandibular Lymph Node/1 (0). No abnormalities found in: Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Plasmacytosis (slight) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node. BACK TO MAIN PATHOLOGY REPORT Page 82/146 T e s t A r t i c l e : telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records A n im al No: SEX: DOSE GROUP: 35 Hale Telomer Acrylat M ACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Animal No: 36 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver K id n ey/1 Spleen days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (minimal) Hemopoiesis, primarily erythropoiesis (minimal) No abnormalities found in: Pancreas, ICidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimal) BACK TO MAIN PATHOLOGY REPORT Page 83/146 Test Article: telomer alcohol/telomer acrylate/ ./pfh s/ pfo s Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 2 1 .Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 36 M ale T elo m er A c ry la t M ICRO SCO PIC FINDINGS (continued) . Animal No: 37 M ACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Mandibular Lymph Node/ .1 Number of Sections less than protocol for Mandibular Lymph Node/1 (0). No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lym p h Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (moderate) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lym p h Node/2. BACK TO MAIN PATHOLOGE REPORT T e s t A r t i c l e : telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 84/146 NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 38 Hale Telomer Acrylat MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Animal No: 39 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) Plasmacytosis (slight) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thyznus, Mesenteric Lymph Node. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted BACK TO MAIN PATHOLOGE REPORT Page 85/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r .1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 39 Hale Telomer Acrylat MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 Spleen Animal No: 40 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Thymus Mandibular Lymph Node/ 1 Inflammatory cell foci (slight) Single cell necrosis (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Exocrine atrophy, focal (minimal) Tubular atrophy, cortical (minimal) Pelvic inflammation, lymphoid (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) No abnormalities found in: Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Lymphocytolysis, increased (slight) Congestion/erythrophagocytosis (moderate) BACK TO MAIN PATHOLOGX REPORT Page 86/146 Test Article: te l o m e r a l c o h o l / t e l o m e r a c r y l a t e / / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propth no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 40 Male Telomer Aerylat MICROSCOPIC FINDINGS (continued) Animal No: 41 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 42 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS - No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT Page 87/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 43 M ale T elo m er A c r y la t MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 45 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 46 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 88/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date .242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 46 Male Telomer Acrylat MICROSCOPIC FINDINGS Animal No: 47 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: SEX: 129 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen No tissues examined days of treatment :29 sacrifice group :recovery group 2 necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :02/12/98 2 No findings noted No tissues examined days of treatment :28 sacrifice group :terminal sacrifice necropsy status :other date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy :05/11/98 No findings noted ' Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) BACK TO MAIN PATHOLOGY REPORT Page 89/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Pfopath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 129 Female T elo m er A c r y la t MICROSCOPIC FINDINGS (continued) Animal No: 130 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver KLdney/1 Kidney/2 Spleen Thymus No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Nod e / 2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) Tubular atrophy, cortical (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid atrophy - involution (minimal) No abnormalities found in: Pancreas, Ovary/1, Ovary/ 2, Adrenal Gland/I, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 90/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prpath no. Date 242933 99010 21.Apr.1999 I n d iv id u a l A n im a l D a ta R e c o r d s Animal No: SEX: DOSE GROUP: 131 Female T e lo m er A c r y la t MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen . Animal No: 132 MACROSCOPIC FINDINGS days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy :05/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lynph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted BACK TO MAIN SATHOLOGI REPORT Page 91/146 Test Article: te l o m e r a l c o h o l / t e l o m e r a c r y l a t e / ,/pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 132 F em ale Telomer Acrylat MICROSCOPIC FINDINGS liver Pancreas S p le e n Animal No: 133 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS' Liver Spleen Thymus ' . Inflammatory cell foci (minimal) . Exocrine atrophy, focal (minimal) H e m o p o ie sis, p r im a r ily e r y t h r o p o ie s is (m in im al) Hemosiderin pigment (minimal) No abnormalities found in: Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminti date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy :06/11/98 No findings noted Inflammatory cell foci (slight) S in g le c e ll n e c ro sis (s lig h t) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (slight) Lymphoid atrophy - involution (minimal) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 92/146 T e s t A r t i c l e : telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services N OTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 134 Female Telomer Acrylat MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver KLdney/2 Spleen Thymus Animal No: 135 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lyraphocytolysis, increased (minimal) No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy :06/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 93/146 Test Article: t e l o m e r a l c o h o l / t e l c m e r a c r y l a t e / / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prbpath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 135 female Telomer Acrylat MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 Animal No: 136 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS L iv e r Kidney/2 Adrenal Gland/1 Spleen Thymus Mandibular Lymph Node/ 1 Inflammatory cell foci (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Plasmacytosis (slight) Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted In fla m m a to ry c e l l f o c i ( s lig h t ) Single cell necrosis (minimal) Interstitial inflammation, lymphocytic (minimal) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) Lymphoid atrophy - involution (minimal) Plasmacytosis (slight) BACK TO MAIN PATHOLOGY REPORT Page 94/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prtpath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 136 Female Telamer Acrylat MICROSCOPIC FINDINGS (continued) Mandibular Lymph Node/ 2 Animal No: 137 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 138 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/2, Mesenteric Lymph Node. days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT Page 95/146 Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e / / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 139 Female Telomer Acrylat MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 141 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 142 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 96/146 T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Pfopath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 142 Female Telomer Acrylat MICROSCOPIC FINDINGS Animal No: 143 MACROSCOPIC FINDINGS M ICROSCOPIC FINDINGS Animal No: SEX: DOSE GROUP: 49 Male MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) BACK TO MAIN PATHOLOGY REPORT Page 97/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ .-i/PFHS/EFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Prpath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE G/ROUP: 49 Hale . MICROSCOPIC FINDINGS (continued) Animal No: 50 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Animal No: 51 MACROSCOPIC FINDINGS . No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy :03/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (m in im al) Hemosiderin pigment (minimal) N o abnormalities found in: Pancreas, KLdney/1, K id n e y /2 , T e s t e s / l, T e ste s/ 2 , A d re n a l G la n d / 1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminali sacrifice necropsy status : planned terminal date of staurt of treatment: 06/10/98 . date of end of treatment: 02/11/98 date of necropsy : 03/11/98 BACK TO MAIN PATHOLOGY REPORT Page 98/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 51 Hale MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Spleen Animal No: 52 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mesenteric Lymph Node Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 No findings noted Inflammatory cell foci (minimal) - Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Congestion/erythrophagocytosis (minimal) Plasmacytosis (slight) Lymphoid hyperplasia (slight) Congestion/erythrophagocytosis (slight) Congestion/erythrophagocytosis (slight) BACK TO MAIN PATHOLOGY REPORT Te s t Article: telomer alcohol/ telomer acrylate/ / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 99/146 NOTOX no. Propath no. Date 242933 99010 2 1 .Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 52 Male MICROSCOPIC FINDINGS (continued) Animal No: 53 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Animal No: 54 MACROSCOPIC FINDINGS . No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/1, Testes/2; Adrenal Gland/1, Adrenal Gland/2, Thymus. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 BACK TO MAIN PATHOLOGY REPORT Page 100/146 T e s t A r t i c l e : telomer alcohol/telomer acrylate/ /ffhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services N OTOX no. Prpath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 54 Male MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver KLdney/1 Spleen Animal No: 55 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Spleen Mandibular Lymph Node/ 1 . No findings noted ' Inflammatory cell foci (slight) Single cell necrosis (minimal) Hyaline cast(s) (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Apoptosis/single cell necrosis, exocrine (slight) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) Plasmacytosis (slight) BACK TO MAIN PATHOLOGY REPORT Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 101/146 NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 55 Male MICROSCOPIC FINDINGS (continued) Mandibular Lymph Node/ 2 Animal No: 56 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver KLdney/1 Kidney/2 Adrenal Gland/1 Spleen Thymus . Plasmacytosis (slight) No abnormalities found in: KLdney/1, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment; 03/11/98 date of necropsy : 04/11/98 No findings noted ' Inflammatory cell foci (minimal) Tubular atrophy, cortical (minimal) Tubular atrophy, cortical (minimal) Hyaline cast(s) (minimal) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Lymphoid atrophy - involution (minimal) Lynphocytolysis, increased (slight) No abnormalities found in: Pancreas, Testes/l, Testes/2, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 102/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. s 242933 Propath no. : 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 57 Male MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 58 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 59 MACROSCOPIC FINDINGS . days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 No findings noted No tissues examined days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 103/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,'PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services . NOTOX no. Prbpath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 59 Male MICROSCOPIC FINDINGS Animal No: 61 MACROSCOPIC FINDINGS Eyes MICROSCOPIC FINDINGS Eyes Animal No: 62 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS . No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 lens: right side Reduced in size No abnormalities were found in any of the other tissues examined Periorbital inflammation due to blood sampling (moderate) (correlates to GROSS finding) days of treatment sacrifice group : 29 : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT Page 104/146 Test Article: telomer alcohol/ telomer acrylate/ / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 63 Male MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: SEX: 145 Female MACROSCOPIC FINDINGS M ICRO SCO PIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 . days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted No tissues examined days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Test Article: telomer alcohol/ telomer acrylate/ ./pf h s/ pfo s Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 105/146 NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 146 Female M ACROSCOPIC FIND IN GS MICROSCOPIC FINDINGS Liver Spleen Animal No: 147 MACROSCOPIC FINDINGS . days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 . No findings noted ' Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal G l and/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminati sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 106/146 T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 9910 2 1 .Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 147 F em a le M ICRO SCO PIC FINDINGS Liver Kidney/1 Spleen Mandibular Lymph Node/ 1 Animal No: 148 M ACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen ' Thymus - Inflammatory cell foci (minimal) Single cell necrosis (minimal) Cystic tubules (slight) Hemosiderin pigment (slight) Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gl and/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphocytolysis, increased (minimal) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gl a n d / 2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 107/146 Te s t Article: telomer alcohol/ telqmer acrylate/ / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 2 1 .Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 149 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Animal No: 150 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy :06/11/98 No findings noted Inflammatory cell foci (slight) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (slight) Hematopoietic cell foci (minimal) Hemosiderin pigment (moderate) Plasmacytosis (slight) Lymphoid hyperplasia (slight) BACK TO MAIN PATHOLOGY REPORT Page 108/146 Te s t Article: telomer alcohol/ telomer acr ylate/ / pfhs/ pfos T e s t S y s t e m : E x p l o r a t o r y 2 8 - D a y G a v a g e S t u d y i n Rats' Sponsor : 3M Toxicology Services N O TOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 150 Female MICROSCOPIC FINDINGS (continued) Animal No: 151 MACROSCOPIC FINDINGS Uterus MICROSCOPIC FINDINGS liver Uterus Spleen . No abnormalities found in: Pancreas, Kidney/1, KLdney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal . date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 left side Cyst(s) No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Cyst(s) present simple serosal) (correlates to GROSS finding) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGE REPORT Page 109/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal Ho: SEX: DOSE GROUP: 152 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Thymus Animal No: 153 M ACROSCOPIC FINDINGS MICROSCOPIC FINDINGS . days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid atrophy - involution (minimal) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy :20/11/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT Page 110/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no'. Date 242933 99010 21.Apr.1999 Individual Animal Data Reeoxds Animal No: SEX: DOSE GROUP: 154 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 155 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 157 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 08/10/98. date of end of treatment: 05/11/98 date of necropsy :20/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 111/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory'28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 157 Female MICROSCOPIC FINDINGS Animal No: 158 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 159 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined d ays o f treatm en t : 29 s a c r if ic e group : re co v e ry group 2 n ecropsy sta tu s :p la n n e d r e c o v e r y d a t e o f s t a r t o f t r e a t m e n t : 08/10/98 d a te o f end o f trea tm en t: 05/11/98 d ate o f n ecrop sy : 04/12/98 2 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT Page 112/146 Teat Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 65 Hale PFHS MACROSCOPIC FINDINGS M ICRO SCO PIC FINDINGS L iv e r Kidney/1 Spleen Animal No: 66 M ACROSCOPIC FIN D IN GS Lungs Stomach Testes days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted In fla m m a to ry c e l l f o c i ( s lig h t ) S in g le c e l l n e c r o s is (m in im al) Midzonal/centrilobular hypertrophy diffuse (moderate) Hepatocellular vacuolation (slight) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (slight) No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 Hemorrhage glandular mucosa Hemorrhage Reduced in size BACK TO MAIN PATHOLOGY REPORT Page 113/146 Test Article; telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor ; 3M Toxicology Services NQTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 66 Male PFHS MACROSCOPIC FINDINGS (continued) Epididymides M ICROSCOPIC FINDINGS Lungs Stomach Liver Pancreas Kidney/1 Testes/1 Testes/2 Epididymides Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Reduced in size - No abnormalities were found in any of the other tissues examined Intra-alveolar blood, necropsy artefact present (correlates to GROSS finding) Vascular mineralisation, focal (minimal) No micro correlate Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Apoptosis/single cell necrosis, exocrine (minimal) Tubular atrophy, cortical (minimal) Seminiferous tubular atrophy (severe) (correlates to GROSS finding) Seminiferous tubular atrophy (severe) (correlates to GROSS f in d in g ) Atrophy (moderate) (correlates to GROSS finding) Aspermia present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Plasmacytosis (moderate) Congestion/erythrophagocytosis (minimal) No abnormalities found in: Kidney/2, Adrenal Gland/ 1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node. BACK TO MAIN PATHOIOGT REPORT Page 114/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no.. : 242933 Propath no. : 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: COSE GROUP: 67 Hale PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS liver KLdney/1 Spleen Mandibular lymph Node/ 1 Animal No: 68 MACROSCOPIC FINDINGS . days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy :03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) - Hepatocellular vacuolation (minimal) Coagulative necrosis, focal (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (slight) Plasmacytosis (slight) No abnormalities found in: Pancreas, KLdney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric nymph Node, Mandibular Lymph Node/2. days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy :03/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 115/146 Test Article: telomer alcohol/telomer acrylate/ . ./pfhs/peos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NO.TOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 68 Male PFHS MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 Spleen Animal No: 69 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver KLdney/1 Adrenal Gland/1 Spleen Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (moderate) Apoptosis/slngle cell necrosis, exocrine (minimal) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: KLdney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Interstitial inflammation, lymphocytic (minimal) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) BACK TO MAIN PATHOLOGE REPORT Page 116/146 Test Article: telomer alcohol/telcmer acrylate/ ,'pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 69 Male PFHS MICROSCOPIC FINDINGS (continued) Animal No: 70 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 Spleen . No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Inflammation, mixed granulocytic/lymphocytic (minimal) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (slight) No abnormalities found in: Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Test Article: telomer alcohol/telomer acrylate/ ,pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services Page 117/146 NOTOX no. Propath n o . Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 71 Male PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver KLdney/1 Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Animal No: 72 MACROSCOPIC FINDINGS Liver . days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Cystic tubules (slight) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Plasmacytosis (slight) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 Enlarged BACK TO MAIN PATHOLOGY REPORT Page 118/146 Test Article: telomer alcohol/telomer acrylate/ V pehs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 72 Hale PFHS MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Spleen Mandibular Lymph Node/ 1 Mandibular Lymph Node/ 2 Animal No: 73 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) (correlates to GROSS finding) Hepatocellular vacuolation (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (slight) Plasmacytosis (slight) Congestion/erythrophagocytosis (slight) Congestion/erythrophagocytosis (minimal) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node. days o f treatm en t : 29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 No findings noted Inflammatory cell foci (moderate) Single cell necrosis (slight) Midzonal/centrilobular hypertrophy, diffuse (slight) BACK TO MAIN PATHOLOGY REPORT Page 119/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PPOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. 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Date 242933 '99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 74 Male PFHS MACROSCOPIC FINDINGS Testes MICROSCOPIC FINDINGS Liver Testes/l Testes/2 Animal No: 75 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 Reduced in size No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Single cell necrosis (minimal) Seminiferous tubular atrophy (moderate) (correlates to GROSS finding) Seminiferous tubular atrophy (moderate) (correlates to GROSS finding) days of treatment : 29 sacrifice group : recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) BACK TO MAIN PATHOLOGY REPORT Page 120/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NO.TOX no. : 242933 Propath no. : 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 77 Hale PFHS M ACROSCOPIC FIND IN GS MICROSCOPIC FINDINGS Liver Animal No: 78 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Id.ver Animal No: 79 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : piaimed recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 ' ' date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 BACK TO MAIN PATHOLOGY REPORT Page 121/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX n o . P ro p ath n o . D ate 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 79 Male PFHS MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS liver Animal No: SEX: 161 Female MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS liver Kidney/1 Spleen . . No findings noted Inflammatory cell foci (slight) Hepatocellular vacuolation (minimal) days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted " Inflammatory cell foci (slight) Tubular epithelial hypertrophy, focal (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 122/146 Test Article: tblomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal Mo: SEX: DOSE GROUP: 162 Female PFHS MACROSCOPIC FINDINGS . MICROSCOPIC FINDINGS Liver Spleen Animal No: 163 MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 ' sacrifice group : terminal sacrifice necropsy status : planned terminal d a te o f s t a r t o f tre a tm e n t: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 123/14S Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOJOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 163 Female PFHS MICROSCOPIC FINDINGS Liver Spleen Thymus Mandibular Lymph Node/ 1 Animal No: 164 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Thymus Inflammatory cell foci (moderate) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (slight) Lynphocytolysis, increased (minimal) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy :05/11/98 No findings noted Inflammatory cell foci (slight) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid atrophy - involution (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 124/146 Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 165 Female PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Pancreas Spleen Animal No: 166 MACROSCOPIC FINDINGS days of treatment :29 sacrifice group :tennined, sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy :06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Apoptosis/single cell necrosis, exocrine (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: KLdney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 125/146 Test Article: telomer alcohol/telomer acrylate/ 'pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. 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Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 166 Female PFHS MICROSCOPIC FINDINGS Liver Adrenal Gland/1 Spleen MandibularLymph Node/ 1 Animal No: 167 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Spleen Inflammatory cell foci (minimal) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid hyperplasia (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular-Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 126/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. : 242933 Propath no. : 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 168 Female PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Animal No: 169 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminalsacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 . No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) Cystic tubules (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. ' days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT Page 127/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 170 Female PFHS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 171 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 173 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 128/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 173 Female PFHS MICROSCOPIC FINDINGS Animal No: 174 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Animal No: 175 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted No tissues examined BACK TO MAIN PATHOLOGY REPORT Page 129/146 Teat Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 81 H ale PFOS. MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Mandibular Lymph Node/ 1 Animal No: 82 MACROSCOPIC FINDINGS . days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy :03/11/98 No findings noted . Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Hematopoietic cell foci (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Plasmacytosis (moderate) No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 130/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal Ko: 82 SEX: Male DOSE GROUP : PFOS MICROSCOPIC FINDINGS Liver Pancreas Kidney/1 Spleen Animal No: 83 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Kidney/2 Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (moderate) Hepatocellular vacuolation (minimal) Apoptosis/single cell necrosis, exocrine (slight) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) No abnormalities found in: Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy : 03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Hepatocellular vacuolation (minimal) Interstitial inflammation, lymphocytic (slight) Cystic tubules (slight) Interstitial inflammation, lymphocytic (minimal) BACK TO MAIN PATHOLOGY REPORT Page 131/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ "VPFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 83 Hale PFOS MICROSCOPIC FINDINGS (continued) Testes/l Spleen Animal No: 84 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Adrenal Gland/1 Adrenal Gland/2 Spleen Mandibular Lymph Node/ 1 Spermatidic giant cell(s) (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment :28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 02/11/98 date of necropsy :03/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Atrophy, cortical diffuse (slight) Atrophy, cortical diffuse (slight) Hemopoiesis, primarily erythropoiesis (minimal) Plasmacytosis (slight) BACK TO MAIN PATHOLOGY REPORT Page 132/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services N o y o x no. Propath no. Date 242933 99010 21.A p r . 1999 Individual animal Data Records animal No: SEX: DOSE GROUP: 84 Male PFOS MICROSCOPIC FINDINGS (continued) animal No: 85 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Animal No: 86 MACROSCOPIC FINDINGS . No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :04/11/98 No findings noted Inflammatory cell foci (minimal) . Midzonal/centrilobular hypertrophy, diffuse (moderate) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 BACK TO MAIN PATHOLOGY REPORT Page 133/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOXOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 86 Male PFOS MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Kidney/1 Kidney/2 Adrenal Gland/1 Adrenal Gland/2 Spleen Thymus Animal No:- 87 MACROSCOPIC FINDINGS . No findings noted ' Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Hepatocellular vacuolation (minimal) Tubular atrophy, cortical (minimal) Hyaline cast(s) (minimal) Tubular atrophy, cortical (minimal) Vacuolation, multifocal in z. fasiculata (minimal) Vacuolation, multifocal in z. fasiculata (minimal) Hemopoiesis, primarily erythropoiesis (slight) Lymphoid atrophy - involution (slight) No abnormalities found in: Pancreas, Testes/l, Testes/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 134/146 Teat Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Teat System : Exploratory 28-Day Gavage Study in Rata Sponaor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.A p r . 1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 87 Male PFOS MICROSCOPIC FINDINGS Liver Kdney/1 Spleen Animal No: 88 MACROSCOPIC FINDINGS M ICROSCOPIC FINDINGS L iv e r Pancreas Adrenal Gland/1 Adrenal Gland/2 Inflammatory cell foci (alight) S in g le c e l l n e c r o s is (m in im al) M id z o n a l/ c e n trilo b u la r h yp ertro p h y , (m od erate) Hepatocellular vacuolation (slight) d iffu s e T u b u la r a tr o p h y , c o r t i c a l (m in im al) H y a lin e c a s t ( s ) (m in im al) H e m o p o ie sis, p r im a r ily e r y t h r o p o ie s ia (m in im al) No abnormalities found in: Pancreas, Kidney/2, Testea/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 04/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Apoptosis/single cell necrosis, exocrine (minimal) Vacuolation, multifocal in z. fasiculata (minimal) Vacuolation, multifocal in z. fasiculata (minimal) BACK TO MAIN PATHOLOGY REPORT Page 135/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 88 Kale PFOS MICROSCOPIC FINDINGS (continued) Spleen Thymus Animal No: 89 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 90 MACROSCOPIC FINDINGS liver .- Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Lymphoid atrophy - involution (minimal) No abnormalities found in: Kidney/1, Kidney/2, Testes/1, Testes/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. . days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrllobular hypertrophy, diffuse (slight) Hepatocellular vacuolation (minimal) days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 18/11/98 Accentuated lobular pattern BACK TO MAIN PATHOLOGY REPORT Page 136/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 90 Male PFOS M ACROSCOPIC FIND IN GS (continued) MICROSCOPIC FINDINGS Liver Animal No: 91 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 93 MACROSCOPIC FINDINGS . No abnormalities were found in any of the other tissues examined Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) (correlates to GROSS finding) Hepatocellular vacuolation (slight) (correlates to GROSS finding) days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :18/11/98 No findings noted Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (moderate) ' Hepatocellular vacuolation (slight) days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 BACK TO MAIN PATHOLOGY REPORT Page 137/146 Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NO.TOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 93 Male PFOS MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver Animal No: 94 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 95 MACROSCOPIC FINDINGS . No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (moderate) Hepatocellular vacuolation (minimal) days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy : 02/12/98 No findings noted BACK TO MAIN PATBOLOG REPORT Page 138/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,'PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. : 242933 Propath no. : 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 95 M ale PFOS M ICROSCOPIC FINDINGS Liver , Animal No: SEX: 177 Female M ACROSCOPIC FIND IN GS ' MICROSCOPIC FINDINGS Liver . Kidney/2 Adrenal Gland/1 Spleen Thym us Inflammatory cell foci (alight) Single cell necroaia (minimal) Midzonal/centrilobular hypertrophy, diffuse (alight) days of treatment : 28 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy :05/11/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Tubular atrophy, cortical (minimal) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) L ym ph oid a tr o p h y - i n v o lu t io n (m in im al) No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGY REPORT Page 139/146 Test Articles telomer alcohol/telomer acrylate/ ,/pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. Propath no. Date 242933 9901 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 178 Female PFOS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/2 Adrenal Gland/1 Spleen Animal No: 179 MACROSCOPIC FINDINGS days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal) Small glands, normal morphology. Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 140/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ VPFHS/E'FOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 179 Female PFOS MICROSCOPIC FINDINGS Liver Spleen Animal No: 180 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen - Inflammatory cell foci (alight) Single cell necroaia (minimal) Midzonal/centrilobular hypertrophy, diffuae (moderate) Hemosiderin pigment (moderate) No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98 No findings noted Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse (minimal) Interstitial inflammation, lymphocytic (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. BACK TO MAIN PATHOLOGX REPORT . Page 141/146 Test Article: tslomer alcohol/telomer acrylate/ -/pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 181 female PFOS MACROSCOPIC FINDINGS M ICRO SCO PIC FINDINGS Liver Kidney/1 Spleen Animal No: 182 MACROSCOPIC FINDINGS days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 NO findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted BACK TO MAIN PATHOLOGY REPORT Page 142/146 Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal Ho: SEX: DOSE GROUP: 182 Female PFOS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Mandibular Lymph Node/ 1 Animal No: 183 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen Mandibular Lymph Node/ 1 Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Plasmacytosis (slight) No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment :29 sacrifice group :terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy :06/11/98 No findings noted Inflammatory cell foci (minimal) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (moderate) Interstitial inflammation, lymphocytic (minimal) Hemosiderin pigment (slight) Plasmacytosis (slight) BACK TO MAIN PATHOLOGY REPORT Page 143/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ j/PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. : 242933 Propath no. : 99010 Date : 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 183 Female PFOS MICROSCOPIC FINDINGS (continued) Animal No: 184 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Kidney/1 Spleen ' No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2. days of treatment : 29 sacrifice group : terminal sacrifice necropsy status :planned terminal date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 06/11/98 No findings noted Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) No abnormalities found in: Pancreas,Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lynph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2. . BACK TO MAIN PATHOLOGY REPORT Page 144/146 Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NOTOX no. Propath no. Date 242933 99010 21 Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 185 Female PFOS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 186 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver . Animal No: 187 MACROSCOPIC FINDINGS - days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted Inflammatory cell foci (minimal) days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98 No findings noted Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) , days of treatment :29 sacrifice group :recovery group necropsy status :planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy :20/11/98 BACK TO MAIN PATHOLOGY REPORT Page 145/146 T e s t A r t i c l e : telomer alcohol/ telomer acrylate/ / pfhs/ pfos Test System : Exploratory 28-Day Gavage Study in Rats Sponsor : 3M Toxicology Services NQTOX no. Propath no. Date 242933 99010 21.Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 187 Female PFOS MACROSCOPIC FINDINGS (continued) MICROSCOPIC FINDINGS Liver . Animal No: 189 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver Animal No: 190 MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted Inflammatory cell foci (slight) Single cell necrosis (minimal) days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted . Inflammatory cell foci (minimal) Single cell necrosis (minimal) BACK TO MAIN PATHOLOGY REPORT T e s t A r t i c l e : telomer alcohol/telomer acrylate/ ./pfhs/pfos T e s t S y s t e m : E x p l o r a t o r y 2 8 - D a y G a v a g e S t u d y iri R a t s Sponsor : 3M Toxicology Services Page 146/146 N O TOX no. Eropath no. Date 242933 99010 2 1 .Apr.1999 Individual Animal Data Records Animal No: SEX: DOSE GROUP: 191 Female PFOS MACROSCOPIC FINDINGS MICROSCOPIC FINDINGS Liver days of treatment : 29 sacrifice group : recovery group 2 necropsy status :planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98 No findings noted Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (minimal) 3M Environm ental Technology and Services PO Box 33331 ~ St. Paul, MN 55133-3331 612 778 6442 * BACK TO MAIN 3M j I i Laboratory Report Report of Data for Exploratory 28-Day Oral Toxicity Study in Rats: Telomer Alcohol, Telomer Acrylate, , PFHS, PFOS Laboratory Report No. FACT-TOX-120.3 Requester Project No. NOTOX #242933 Testing Laboratory 3M Environmental Technology & Safety Services 3M Environmental Laboratory Fluorine Analytical Chemistry Team (FACT) 2-3E-09 935 Bush Avenue, S t Paul, M N 55106 , Laboratory Contact . . Kris Hansen, Ph.D. Bldg. 2-3E-09 P.O .B ox 3331 S t Paul, M N 55133-3331 Phone: (651)778-6018 FAX: (651) 778-6176 Requester M arv Case and Paul Lieder 3M Company Toxicology Sendees ' 220-2E-02 S t Paul, M N Report issued 6/ 11/99 BACK TO MAIN 3M ENVIRONMENTAL LABORATORY REPORTNO. FACT-TOX-J20 This study was designed to preliminarily assess the toxicity o fvarious test articles to the female Sprague Dawley r a t Test animals were dosed by oral gavage at the M ow ing levels: Group 2: Telomer Alcohol Group 3: Telomer Acrylate Group 4: Group 5: PFHS G roup6:PFOS 30m g/kg/day 30 mg/kg/day 30 mg/kg/day lOmg/kg/day 3 mg/kg/day W ithin each group, 8 anim als were given test material each day for 28 days, and then sacrificed. Three anim als were allowed to "recover" (no test material) for 14 days before sacrifice; three animals were allowed to recover for 28 days before sacrifice. . Liver samples collected from the sacrificed animals were sent to the Environmental Lab and analyzed quantitatively for perfluorooctane sulfonate (PFOS) and other perfiuorinated, sulfonate-based or carboxylic ad d based metabolites. (A M list o f acronyms and formulas is attached in Attachment B). Tissue samples were homogenized (liver) and extracted using an ion-pairing extraction procedure. The .extracts were quantitatively analyzed using high-pressure liquid chromatogiaphy/electrospray tandem mass spectrometry (HLPC/ESMSMS), and evaluated versus an extracted curve. Analytical details are available in the study binder maintained by FACT. The presence or absence ofknown fluorochemical contaminants and metabolites is determined by inspection. Six perfiuorinated carboxylic adds were identified in the liver sample extracts derived from both group 2 and grog) 3 animals (see Attachment D). . Forgroup2 and group 3, livers from male and female animals in both groups ofrecovery animals showed a . decrease in the concentration ofPOAA The concentration o f two other carboxylic ad d based . fluorochemicals measured quantitatively (M513, M513) did not show as significant a decrease over the recovery period. The concentrations ofthree other carboxylic add-based compounds could notbe determined, as standard material is not available. Other perfiuorinated or partialfy-fluorinated metabolites may be present in the rat liver samples analyzed, but these compounds cannot be confirmed without standard m aterial . Relative to PFOS levels in the corresponding group 6 animals, (grog) 4) and PFHS (grog) 5) levels determined in liver samples showed a dramatic decrease in the day 14 and day28 recovery groups. W ithin each group, samples showed a relatively large standard deviation which may indicate variability in dosing or in metabolism o f the test compound. 's W ith the exception ofPFOS-dosed animals (group 6), there were dramatic differences in target analyte levels in livers o f m ale and fem ale animate For example, livers from 14 day recovery group 5 females animals were determined to contain two and a halforders o f magnitude less PFHS than the corresponding male animals. . Although rigorous quality control measures and 3MEnvironmentaI Laboratory Standard Operating Procedures were M ow ed, die acquisition of this data was not necessarily collected according to applicable Good Laboratory Practices. This data is not suitable for distribution to ary governmentagency in defense of product suitability or safety. Data presented here is the highest quality data available at this time. This summary should not be considered to be a complete inventory of all metabolites associated with the test m aterial Analyte measurements were made only on those compounds deemed by FACT as likely to be . present Certain other compounds have been identified but cannot be quantitated as standard material is not available. . BACK TO MAIN 3M ENVIRONMENTALLABORATORY REPORTNO. FACT-TOX-120 Liver samples arrived frozen at the Environmental Lab on 2/3/99. A chain-of-custody was not submitted with the samples, but was initiated upon receipt by die Environmental Lab. 1 ] A nalytical m ethods 1 FACT-M-l.Q, Extraction o fPotassium Per&uooctanesulfbnate or Other Anionic Fhiarochemical Surfactants from Liver for Analysis Using HPLC/ESMS . FACT-M-2.0, Analysis ofFluorochemicals in LiverExtract Using HPLC-EIectrospray/Mass Spectrometry Sam ple p rep aratio n -aq u eo u s sam ples, HPLC7ESMSMS: ion-pairing extraction . . *. Analyte is extracted from a sample matrix with ion pairing reagent [tetrabutyl ammonium hydrogen sulfate (TBA)] in a pH-controlled environment The cationic reagent selectively targets anionic fluorochemicals. Once the anion-TBA pair is formed, the analyte is transferred into a non-polar organic solvent (m ethyl-tert- butyl ether), dried, and reconstituted in methanol for MS analysis. HPLC/ESM S and HPLC/ESM SM S: fo r detailed qualitative woric . In HPLC, an aliquot of extract is injected and passed through a reverse phase liquid chromatographic column. Based on the affinity o f the analyte for the stationary phase in the column relative to the liquid . mobile phase, die analyte is retained for a characteristic amount of time. For example, in a standard 1 solution PFOS may elute at 5.0 minutes. Retention limes between a standard PFOS solution and the analyte extracted ftom liver in this analysis were matched on the HPLC system to within 1%. .. J3 Following HPLC separation, ESMSMS provides a rapid and accurate means for analyzing a wide range of organic compounds, including fluorochemicals. Electrospray, an ionization technique used primarily for detection o f molecular ions, is generally operated at relatively m ild temperatures. Molecules are ionized, and a primary ion, characteristic of the analyte, is selected. This ion is bombarded with high-energy gas; subsequent collisions create smaller secondary ionic fragments unique to the primary ion, which are detected. v For example, for PFOS (CgFpSQO analysis, ion 499 is selected as the characteristic primary ion. This ion ] is fragmented into other ions such as 80 amu (corresponding to SO3"), and 230 atm (C3F6SCV). Each o f these secondary fragm ents is detected and can be used to differentiate PFOS from other compounds that might have the same characteristic 499 amu primary ion, but different chemical compositions and secondary ion fragmentation patterns. I 5.1 Calibration J A mid-level, extracted matrix calibration check standard is analyzed every 5-10 samples to monitor instrumental drift Calibration check standards are compliant if instrumental response is within +/- 30% o f the expected value. i BACK TO MAIN 1 I 3MENVIRONMENTAL LABORATORY REPORTNO. FACT-TOX-120 Quantitation of the target analytes is based on linear regression analysis (weighted 1/x, if appropriate) o f two extracted matrix curves bracketing each group of samples. Quantitation of each analyte is based in the response of one or more specific daughter ions using the multiple response-monitoring mode o f the instrum ent . 5.2 Blanks Two extraction blanks, utilizing water as surrogate matrix, are extracted with each batch of sanqdes. Additionally, two samples ofblank matrix; used for the extracted calibration curves, are extracted along w ith each batch of samples and curves. Blanks are compliant if no target analyte is detected above the limit ofdetection for a specific analyte. In this study, all blanks were compliant . ` 5.3 Surrogates .' Tetra-hydro perfluorooctane sulfonate is used as a surrogate in this analysis. Surrogate response is monitored to confirm gross instrum ental failure, if necessary. 5.4 Matrix Spikes Duplicate matrix spike analyses utilizing all target analytes were prepared and analyzed for one control animal. Except as noted in the results table, recoveries were within foe acceptable range o f 70-130%. Recoveries outside this range may be re-evaluated. 5.5 Laboratory Control Samples Laboratory Control Samples are not a component o f this study. ! Six perfluorinated carboxylic adds were identified in the liver sample extracts derived from both Group 2 and Group3 animals (see Attachment D). ' J For group2 and group 3, livers from male and female animals in both groups ofrecovery animals showed a decrease in the concentration o fPOAA. The concentration o ftwo other carboxylic arid based fluorochemicals measured quantitatively (M513, MS13) did not show as significant a decrease over the ' recovery period. The concentrations of three other carboxylic arid-based compounds could not be j determined, as standard material is not available. Other perfluorinated or partially-fluorinated metabolites may be present in the rat liver samples analyzed, but these compounds cannotbe confirmed without standard m aterial. x Relative to PFOS levels in the corresponding group 6 animals, (group 4) and RFHS (group 5) levels ] determ ined in liver samples showed a dramatic decrease in the day 14 and day 28 recovery groups. W ithin each group, samples showed a relatively large standard deviation which may indicate variability in dosing or in metabolism ofthe test compound. . W ith the exception ofPFOS-dosed animals (group 6), there were dramatic differences in target analyte levels in livers o f m ale and female animals. For example, livers from 14 day recovery group 5 females animals were determined to contain two and a halforders ofmagnitude less PFH5 than the corresponding male anim als . .j These results indicate that ingestion of each o fthe test materials leads to some accumulation o f metabolites in the liver. Relative rate ofclearance can be determined. BACK TO MAIN 3MENVIRONMENTAL LABORATORY REPORTNO. FACT-TOX-120 Samples and extracts willbe retained for future evaluation, if necessary. 9.1 A ttachm ent A : Table of Results o f Analysis o f L iver Samples 9.2 A ttachm ent B : Table of FACT acronyms and form ulas 9.3 A ttachm ent C : Graphical Results by Group and Gender 9.4 A ttachm ent D: Chromatographic results ofPFC A investigation in Group2 and Group 3 9.5 A ttachm ent E : Chain o f Custody . 9.6 A ttachm ent F : Analytical Data .' LisaClemen,AnalyticalChemist Date / 135----- -------------- `________ 6// Kris Hansen,P hD , Principal Analytical Investigator D ate .x 3 M Environm ental Laboratory Fluorine A nalytical Chemistry Team fFACT) FACT-TOX-120, R at Livers 28-D ay O ra! Tox Study by D aily Gavage in Rats 1 i PFOS (C8F 17S03`) 1 (Female) Day 1 Day 14 Day28 G l 0.086+/-0.046 0.102+/-0.020 0.329+/-0.060 1 G6 377+/-31 240+/-25 191+/-15 ] (Male) Gl Day 1 Day 14 Day28 0.133+/-0.030 0.255 +/- 0.122 0.099+/-0.007 G6 4 1 9 + /-8 6 3 0 9 + /-3 4 237+/-25 1 I PFHS (C6F I3S03-) (Female) Day 1 Day 14 Day28 j Gl <MDL <MDL <MDL G5 97.0 +/-14.7 0.26+/-0.12 0.03 +/- 0.01 I (Male) Day 1 Day 14 Day28 G l 0.055 +/- 0.020 0.129 +/- 0.088 0.089+/-0.019 ] G5 327+/-52 61.9+/-11.7 36.3+/-7.4 J 3 ( / (Female) D ayl Day 14 Day28 J Gl <MDL <MDL <MDL G4 0.37+/-0.38 0.02+/-0.00 <MDL 1 (Male) Day 1 Day 14 Day28 ! Gl <MDL < MDL <MDL G4 3.09+/-1.58 0.126+/-0.084 0.025 +/- 0.015 I j 3M Confidential BACK TO MAIN 3 M Environm ental Laboratory Fluorine A nalytical Chemistry Team fFACTl FACT-TOX-120, R at Livers 28-D ay O rai Tox Study by D aily Gavage in Rats POAA (C7F 1SC02) (Female) Gl G2 G3 (Male) Gl G2 G3 Dayl <MDL 0.164+/-0.035 0.112+/-0.039 Day 14 <MDL 0.016 +/-0.010 < MDL Day28 <MDL <MDL <MDL Dayl <MDL 6 .5 9 + /-2 .1 6 8.64+/-1.48 Day 14 <MDL 3 .5 9 + /-0 .6 7 3.36+/-1.65 Day28 <MDL 1.76+/-0.23 1 .2 6 + /-0 .4 4 MS13 (C,F19C02) (Female) Gl G2 G3 (Male) Gl G2 G3 Dayl <MDL 1.69+/-0.18 1.20 +/-0.30 Day 1 <MDL 1.91+/-0.88 1.78+/-0.31 Day 14 < MDL 1.36+/-0.02 1.20+/-0.09 Day28 <MDL . 2.24+/-0.22 0.812+/-0.188 Day 14 <MDL 1.73+/-0.46 1.08 +/- 0.26 Day28 <MDL 1.52+/-0.32 0.801+/-0.148 M613 (Cn F 23C02-) (Female) Gl G2 G3 . (Male) Gl G2 G3 . Day 1 <MDL 0.117+/-0.020 0.029 +/- 0.017 Day 14 <MDL 0.085 +/-0.019 0.035 +/-O015 Day28 <MDL 0.101+/-0.012 0.036+/-0.011 Day 1 <MDL 0.087+/-0.015 0.0622 Day 14 <MDL 0.107+/-0.041 <MDL Day28 <MDL 0.106+/-0.017 <MDL * * C9, C ll and C13 perfluorinated carboxylic acids also detected in G2 and G3 animals 3M Confidential BACK TO MAIN* L. 28-Dsy O rai Tox Stady by DaUy Gavage In Rata Stady: frodaci Nttrti ufTcn Sakatmcc): Manta: DatcoTExtractiaVAnalysC Data o f Asalyab/Ajulytt: Otte * f Data Radaction/AMlyM SampIcDaU TOX-I21 Exflomory 29-Day Orai Teoddty Slady by Dafly Garage fa latti T-7023, T-7024, T-7022, T-7032, T-62W MMUva Filrnnar FACT-M-I.O k FACT-M-2.0 K-Sqund Vaine: Sonp 020199, Madrine 04109, A ad Si 06349 Slopc M taLynM 2/22/99 RWW/SAH 02/23/99, 03/01/99, 03/11/99, 03/25/99HOJ/MEE/DRB 02/25/99, 03/01/99, 03/12/99, 05/26/99 MEE/DRB/KJH/HQJ SceAltacfaacais S e e A lU d n cate See Attachmcal* Sm Attachouatt . RATUVER Cm f S u *kl Irtrtrtri W t F rS Telai Avana! Dnac 1 Cale. Cane afPPOS rt rt Mctbod Blk H2 0 B&-I NA <MQL <MDL MtifcodBlk H20 BIk-2 NA <MDL <MDL Matrix Blk TGSSTK b S T HA <MDL <MDL . Mania Blk Q C - 2 * * Ratti! Uvcr Blk-2 I06P HA 0.9240 <MDL 25 <MDL UH I06P 0.9240 250 *4% Q C - 250PI* I 10P 0.9242 69 23% tlOP 0.9242 99 33% Cranpl 97F 03221 190 1190 0.0at|/k|/day Cannai 91F 0.4339 673 10672 99P 03106 76.1 10761 100P 039*4 64.9 10649 I01P 0.6370 9007 9.01 102P 0.4313 763 - 10765 103P 0.4961 653 10655 104P 0.1116 62.0 10620 Cranpl 105P 0.6936 119 1119 O.Oaw'kg/day I06P 0.9240 toc 1106 Recovery 1- 14Daya 107P 0.4359 10.6 10606 G ranfi 109P 0.4UO 344 1344 lOmffkg/day 1IQP 0.9242 379 1379 Recavay 2-21 Daya 11IP 0.9429 263 1263 G ranfi 113P 0.3506 413 10412 30.0atfAg/day IMF 0.6495 317 10337 T doacr Akohol 1ISP 0.6769 37.1 10371 u f f 0.7235 343 10366 1I7P 0.6534 11.6 1 M I6 h i p 0.4162 56.9 10569 1199 0.5791 310 10360 1209 0.4295 293 10295 G ranfi 121P 0.1372 35.6 10356 M Om fftg'dty 122P 06291 35.4 10354 Recovery 1 14Davi 123P 03660 <7.3 10473 Craapl 125P 0.6074 433 0.0452 30.0 B/kf/day Recovery 2 - 2 Daya 1269 I27P 0.96H 0.6066 515 510 10515 10520 G ranfi 129P . 014161 343 10342 30.0 mffkf/d*y Tdaner Aayiata 1309 I3IP 03012 0.7303 M .4 . 314 10364 10516 132P 0.5990 413 10415 1339 0.3111 494 . 10496 1349 0.6131 453 / 10452 1359 0.5169 317 10357 1369 03799 46.1 10461 G ranfi 1379 0.6064 17.9 10179 31 0nffka/My Recovery t - 14Daya 13BF 139P 0.6235 19.4 10194 0.5660 43.9 10439 G ranfi I4IP 0.6693 414 10424 3 1 4 n ffk f/ d ay I42P 0.7953 413 10411 Recovery 1 -21 Daya 143P 0.7363 403 10402 LA** 10a(/g,M513 -S O R 100nf/g, M613- 50a f ff Metfand Detection Lfaak (MDL): F F O S -2 3 * f c F O A A - 5 a | fr MS13- 2S a a d S O ^ fe M 613-25afff Avance rras art <MDL MDL 15% 26% 10063* 130 1102 0329 10443 10394 10519 10429 10271 10413 Data Entcrnd/Aariyat: Date Verified/Aaalyat: 02/26/99. 03/0C/99, 03/22/99. 05/2699 LAC 06M /99, 06/DW99 BAD M BSD SUL Dar. <MDL <MDL 3% 36% 53.4* >10461* 263 3.15 193 10196 113 10397 36.6 10171 173 0.00664 12. 100667 15.0 100644 53.9 10146 2.70 100112 - POAA Caie. Cane. Tata! Amoea! af POAA Avance POAA BSD SdDcv. rt ...... <MDL art <MDL rt <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL 219 73% 267 19% 1% 20% 222.90 75% 172.72 56% 66% 25% 0.00 <MDL 100 <MDL 0.00 <MDL io o <MDL 100 100 100 0.00 0.00 0.00 0.00 100 0.00 100 <MDL <MDL <MDL <MDL <MDL <MDt <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL 135 1135 115 111S 16) 1163 160 1160 115 1115 196 1196 223 0323 213 139 1139 1164 9.0954 24.3 10245 433 100453 653 17.4 10174 10153 10101 0 <MDL 0 <MDL 0 <MDL <HDL <MDL 663 10665 105 1105 95.0 10950 * 166 1166 124 1124 90.6 10906 170 1170 35.9 75.1 10751 1112 10390 100 <MDL 100 <MDL 0.00 <MDL <MDL <MDL 100 <MDL 100 <MDL 100 <MDL <MDL <MDL * Val w u detonalaed ao collier and wac no! hcladcd a Icae cikalriiana. N R -N o t Rqwrtcd N /A-N ot Applkabl PFOS-Ftfflaoraoctaaaalfi POAA PcfflaoroocHaoHc M513- C 9P19COO- M613 CI IF23000- Cale. Cane.- rt <MDL <MDL <MDL <MDL 122 193 122 193 100 0.00 6VALUEI 100 100 0.00 . IVALUet VALUEI 6VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI* VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI (VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI ' Tata! Amena! af art <MDL <MDL <MDL <MDL 41% 65% 41% 65% io PI* <lpp HR <ippk I0 PP* <10 PI* NR HR HR HR HR HR HR HR HR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR Avance rt <MDL <MDL 53% 53% NR NR NR NR NR NR NR NR NR RSD ftd.Dev. <MDL <MDL 46% 49% NR NR NR NR NR NR NR NR NR PACT-M-2.0 7.0 TOX42 1livm.xh 4/1099 5:12 9*4 ***** frodiciH i te (ra> M w a ) : Macrc AnalytkalEyytSyncroKwkB DMcerExtnctWABilyst: DatecfAaalyw/Aeaiy*: DMcofDauRcdcctioa/AnfyaC SuapleData FACT-TOX-120 2 W J y O ra i Tox S tody by DUy G ir a te fai R i t i TOX-120,Expfcntory2t-DayOraiToridcyStady9yDeflyQnra|tiaRata T-7023,T-7024,T-7022,T-7032,T-6295 RrtJJwr SeItalotifi* FACT-M-1.0 k FACT-M-2.0 R-SquredVatoeScfAtlachmcatt SMp 0201, lladdM 04I0W; Aactia 00491 M anLywl! imm rww/sah v a r a n e ov o i m , ov u m , o s a s m hoj/meb/d rb a i m m , ovoc/*, o v ia m , t a n t m mee/drb/k jh h o j BACK TO MAIN RATUVER P ro MdhoORik IM ifallk QC-250pp4 QC-250ppfc 0.0 M$fc$/dMy Cootrol .om$rkttf Recovery i . 14 D m Graap | <20a*/k*/d*y Recovery 2-21 D m SO.Oaf'ksMay Tdorocr Alcohol 30.0*k*/day Recovery 1 14 D m GrmpZ SO.Oroftyday Recovery 2-2* D m 3R0 **d*y Tdoacr Aoytaia J0.0*A**ay Recovery 1 - 14 D m .O o iM iy Recovery 2-21 D m UmpUI H20 Blk-1 H20Wk-2 M M lL lnrB ST Rabhb Uver Blk-2 I06F 1I0P 110F 97F nr 99T loop* 10IP 102F 103F IMF I0SF 106F 107F 109F 110F I11F I13P 1UF 115F 116F 1I7F h ip UFF 120P 12IF 122F 123F I25F 126F 127F IMF 1JIF IMF IMF IMF IMF IMF 137F 13IF 139F I41F 142P wr 1 NA NA NA NA 0.9240 09240 0.9242 0.9242 05221 0.4339 OJIO 0.59*4 0.6370 0.4313 0.49*1 0*11 0.6934 0.9240 0.4359 0.41*0 0.9242 0.9429 0.4495 0.4769 0.7235 0.6534 04162 09791 04295 0.1372 0.6291 09660 0*074 0.9*12 060*6 0.3012 07303 09990 0JI11 0.4131 0.536* 09799 0*0*4 06239 096*0 0.6693 07993 07319 CMc. Cea. 0% i. ~,*MDl.; <MDL '< .... . " ' ^ ' <MDL -, '' . I M O 'flOdl W $ ii - ?i,V 0t00^* v '.^hOOOt^- v*. l* 3 m * * M * ^ i^ f^ T O tfiSF & J S S S S lillii ;JM o o % .n ? * '1 aCPFM* n /i *MDL <MDL <MDL MDL 21494 94194 OS OS NR NR NR NR NR NR NR NR NR . NR NO NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR . FFH *fr <MDL <MDL 36694 OS NR NR NR NR NR NR NR NR NR DataEatered/Auly: ouitm,0300/99,mmm,05/24/99la DateVaifiaMntyat: OMM/99,069*99EAD RSD StODev. <MDt <MDL IOS *DIV/(H NR NR NR NR NR NR NR NR NR M51J Cale. Ceac > rt <MDL <MDL MDL <MDL 274 212 179 162 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.0 0.00 0.00 0.00 1791 ' 1*16 1671 1556 1353 164! 171* 1931 1366 1361 2396 2323 1991 1015 1446 72 1444 7*5 1123 1646 1252 119* 1302 1113 1014 7*2 640 (H ill V i <MDL <MDL <MDL MDL 92S 9494 . 6094 54% <MDL <MDL <MDL <MDL <MDL 04DL <MDL <MDL <MDL <MDL <MDL <MDL <MDL :-:;1-W 7 'W ts $ { 140 133 1.99 1.01 1.45 0.(72 1.44 0.7*5 1.12 1.05 I.2S IJO L30 1.11 1.01 0.7*2 . Averti* MS13 a <MDL <MDL 93% 97S <UDL <MDL <MDL * o a* ** L69 * 00 U6 2J4 IJO 1J* 0.112 NR-NetReportad WA--NotApplfeaM* FFOS-Ferfkorooctroaal FOAA-PcrIUorooctiooaU M51J - C9F19COO- M413 - CI 1P23COO- "fasi H tn <MDL <MDL 2% 10% MDL <UDL <MDt I0L7 Olili 1.12 0.02 f.9 OJIO 213 OJOS 7.17 0.0941 23J OJIS - 'W ii Clk.CMC. Vi <MDL MDt <MDL <MDL 267 314 272 241 0.00 0.00 0.00 0.00 M 0.00 &00 0.00 0.00 OiOO 000 aoo 0.00 Tefal Aroatei ' ef MfU <MDL <MDL <MDL 19% 105% 91% 1% <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDt 142 121 14 112 120 109 106 *3. 101 <SJ >9.0 974 114 90.5 213 32J 27J 30.7 4.74 644 25.9 22.7 19J 36.4 49.7 43J 4U 23.1 0.142 0.121 * 0.14 0.112 0il20 aios Olio 0.0*36 0.103 0.06S5 00*50 0.0974 0.114 Q.Q2t9 0.0321 0.0272 0.0307 0.0047 ' 0.0644 0.0259 0.0(93 0.0364 .M97 0.0433 OlMIS 010231 Avcrai M6I3 aii7 01004 noi 0.0351 0.0361 uso StO. Dcv. 12% I7J 22.3 11.1 &0119 51.2 43J 29.7 0.0107 FCT-M-2.0 PwrfVenJaiT# TOX-120-tvw.xl* *nom 5:12m ----------- FAC---------,<-110 , 28-Day O ral Tax Study by Dally G iv a te la R ati M a d N a n b a tfc * SnWmce): HcAod/RcvUa: Analytical Equipment SyUM N n A as Date of Extraction/Aailyit: DMeof Aulym/Aaalyat: Dato of Data Rcdaction/Aaalyst: Sam ple Data TOX-120, Exploratory 24-Day Oral Toxidty Stady by Dafly Gavajg a Kata T-7023. T-7024, T-7022, T-7032, T-4295 Rat liver FACT-M-t.O k FACT-M-XO R-Sqaand Vaine Madetiat 041091, Amelia 0C49I, md Soap 020199 Slope MaacLyax3.l Y-btorccpC 1/22/99 rw w /sah 02/24/99, 03/1 \ m , a v i 4/99. 05/25/99 h oj/m e e /d rb 03/0099,01/12/99,' 03/17/99, 05/26/99 DRB/KJH/HOI RAT LIVER Cmp Dam Saaplef Extracted WL S Method Bit Method Blk Matrix BU Matrix. Blk QC-lM pp* iJ-iM pi* Cranp4 Cm p4 30.0 np'kg/day Recovery 1 - 14 Day Graap4 30.0 Bg/kf/day Recovery 2 >21 Day* C r ,l " 10.0 B|/kf/tfqr PPHS Croups 10.0 Bf/kf/diy Recovery I - 14 Day* GroopS 10.0aifAfUay Recovery 2 -21 Deyi C rm p4 3.0 awtyUay pros C napf 3.0 np/kg/dsy Recovery 1-14 Daya Groop 4 ).OH0ka/day Recovery 2 21 Days K20 Blk-1 H20 Btk-2 Rebbit Liver Blk-1 Rabbit LiwBlk-2 106P I06P HOP nor 14SF 144P I47P I4IP 149P HOP HIP H2P 153P 1S4P 133P 137P HIP 139P 161P l2P I43P 14P TOP I66P 147P IMP 169F 170P 171P 173F 174P 173F 177P I7IP 179P HOP HIP H2P TOP IS4F IMP IMP H7F IMP 190P 191P HA HA HA HA 0.9240 09240 09242 09242 0.3643 04113 04457 04499 04097 04414 0.5474 04341 0.1374 07221 01019 O lili 0.5430 0.9194 09199 06444 04107 04779 07719 0.4252 04732 04374 05394 07757 0.5471 0.4764 0.7937 04424 07152 04423 05462 01106 J im 05940 04)12 04207 1.0570 0.4345 07564 04749 04442 09140 PFOS Cale. Cne. n ft <MDL <MDL <MDL <MDL 251 250 49 99 144 114.7 90.2 130 1017 103.0 150 111.4 77.4 5 71.7 142 96.1 142 99.1 44.7 774 117.4 74.2 7X7 1207 903 65J <34 41.1 544 444 115.9 345911 3S307S 314730 343395 422I2S 404243 343202 325352 214147 247144 2353SI 112144 143230 201253 Tatal Aafenat af PPOS 0* <MDL <MDL <MDL <MDL 16% 4454 2354 3354 0144 01147 00902 0130 01027 01030 0150 am i 00776 0135 00717 0142 00944 0162 0.0991 00447 0.0772 01174 00742 00727 0121 00903 0.0459 00435 0.0411 0.0545 00445 0116 364 343 347 343 . 422 / 4M 343 325 2H 244 235 142 143 204 Avance PPOS a rt <MDL <MDL 15% 21% OHSS 00944 0140 00094 0.0571 00734 377 240 191 RSD ~ Std-Dev. <MDL <MDL 00304 0354 I7.S 0.0211 36.9 00350 201 00374 23.6 00212 m OOI33 500 00344 039 31.6 105 25.2 7.72 I4J POAA Cale Cane, a rt <MDL <MDL <MDL <MDL 219 247 223 173 0.00 OOO OOO 000 0.00 OOO OOO 0.00 OOO 000 000 0.00 000 000 000 0.00 000 000 000 000 000 000 OOO 000 0.00 000 0.00 000 000 000 000 000 000 000 000 000 000 000 000 000 000 000 Tetal Aanaat af POAA > rt <MDL <MDL <MDL <MDL 73% 19% 75% 54% <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MOL <MDL <MDL <MDL <MDL <MOL <MDL <MDL <MDL MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MOL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL Average POAA V i <MDL <MDL tl% 44% <MDL <MDL <MDL <MDL <MDL <MDL <UDL <MDL <MDL Method Defection Limb (MDL): P F 0 S -X 5 * lX 5 ap feP O A A -tX S affe J a r t . PPHS - 3 af/g. M513 - JOa r t . M61J - 3 a r t N/A-N ot Applicable prosPOAA- DafeVoillcd/Aaaly; 2/30. 03/109, 3/17/99,03/22/9. 05/209* LAC 000099. 000099 EAD PPHS MSI3 C9F19COOM6I3-C11F23COO- BACK TO MAIN RSD StADcv. <S4DL <MDL 0199 Cric. CMC V WS-JUDL .<: K c<m d l . ,*<. IM . t o * 0254 s ? 14. ;y . .`;IM ,;V a& cm it. S filK i <MDL w m m & i' . `"r '4 0 1 'V <MDL ^ 1 4 .1 0 , , <MDL s ii OOO <MDL <MDL *`^ : oooiVh ^ o o o /i -V <MDL W S*m 0! ISS W iA e.V 'f Sw | S # ,0 0 r - 'i < V . (t <MDL <MDL <MDL fW !. .o o M m & w itsn . % ^ 000'k.iL ^ ' AO.ib.' fw y m w B Sftio.tyv' rt <MDL <MDL <m d l <m d l 41% 45% 41% 45% 0140 00409 OH4 0321 1.19 <MDL 0291 0401 00163 0.0144 00115 000449 HR HR HR HR HR HR HR NR NR HR HR HR HR NR HR HR NR HR HR HR NR HR HR HR HR HR HR NR HR HR V i <MDL <MDL 53% 53% RSD Bid. Dev. <MDL MDL 0454 0454 0375 00145 000449 101 0377 11.9 000194 PDfWM MMV/M HR NR HR HR HR NR NR HR NR HR NR HR PACT-M-2.0 Excd Vcrtiea 7.0 TOX-12(Mivcr.xJa 01099 3:12 PM sudr FWdaal W ih n c r n l t K - f Manie MattoMarMa* Aaalyllcal gyipT n l tyn m Neifcer D auofExnaenoa/Aaalyat Dalao tAaalyiia^Aaalyit Dm TOn M M wM aalyil: SaaipU Data RATUVER C rw f Dm MctborfWk Mcthodl Blk Matrix Blk . Matrix Nk q c - im h * C retf 4 3aoafOfu*r Crampi 30.0 mp\pfKf Recovuy 1* MDayg Cm p4 JO O au ty riay Recovery 2 - 21 Dayi C rrap f IM oftyH qr PFHS C noS lOOa^/kffeay Recovery 1-14 D a IO0 f/kf/riey Recovery 2-21 D a j.o*tfkpftr PPOS G rafi 1.0 Mf/kf/day Recovery 1- 14 Da ).0fcU iy Recovery 2 - SS Dry* Saapla ff H20 Blk-1 H20B1H lUMrit Uvw Blk-1 RabMl Livo Btk-2 104F 106F H0P IlOP 14SP 14<P 147F I4P I49P 190F 151P I52F 1S3F 4P I55P 157P I3IP UPP ' 19P I70P I71P I73P 174P 175F I HA HA HA HA 0.9240 0.9240 09242 09242 02443 04113 04437 044 0.4097 0.641 0447 04341 0157* 07221 01019 O titi 09430 0.919 O ff 0M4 01107 04779 07719 04292 04732 04371 09394 07797 0.9471 0176 07937 0.442 I7IP I79P 1MP 1I1P H1II2PP IMF ItSP IMF ft7F IMF IMF 1*1F 04423 09462 0.110 07104 09940 0.4312 01207 1.0370 01343 07S4I 04719 0.44X2 0.9ISO Ifa tolD M u toU rtp iD t)! UiMM i-- .i PAG______J-120 i______ _ _ 2S-Day O rai Tox Slady by Daily Garage la Rata TOX-IM,Ezpionbxy2t-DayOraiToxicityStadyY t DailyGavine ia Rau T-7023, T-70M,T-70B , T-7032. T-UM dal tirar m an S a tinto ri/bt PACT-M-1J>k FACT-M-2.0 R-&parca VaM:Sae Anactacau MadaliaaMlOM,AaKia0634M,aadSop020ir9 Stopc ' MaaaLynl.1 Y-Mampi; a3w/w2uXw9/tt,,RmaWvniWiw//S9A>),.Hatvaimi/a,,aanmsiia hovmee/d u Duaumm PFHS C klcC w c. afe . * <MDL MDL ~ , <MDL ' - - <MDL; m sm M utM *435 1041*4 94742 91913 114992 S9U1 114702 76152 393 169 22 374 274 31. :n:.''oOMo0o0i''tlPVo*O00OJM OOO u s ti IIIIIili Tauri A n u t rrpH s rt <MDL <MDL <MDL <MDL OH OH OH OH NR HR NR NR HR HR HR NR HR NR NR NR NR NR 4. 10 94.7 91.9 117 19.1 119 76.2 0393 0149 042 00372 00272 0031 NR NR NR NR NR NR NR NR NR NR NR NR NR NR Avance PFHS fe MDL <MDL OH OH NR NR NR RSD SU-Dot. MDL MDL DfVti! 4D1V/W NR NR NR M513 Cale. Case. mt/t <MDL MDL <MDL MDL 27 212 179 162 OOO OOO OOO 0.00 OOO OOO ooo ooo 0.00 0.00 0.00 ooo ooo 0.00 8c iSe88e8e888l 97.1 0263 00120 194 14.7 444 O li 19.7 0.00302 o0.o0o0 0.00 ooo ooo ooo 88888888 NR NR ooo 0.00 NR NR 0.00 0.00 ooo NR NR 0.00 N/A-NotApp&ibU Tata! A m u t al MS13 rt <MDL MDL <MDL MDL 92% 94% 60% 54% <MDL <MDL <MDL MDL <MDL <MDL <MDL <MDL <MDL <MDL MDL MDL <MDL <MDL MDL MDL MDL <MDL <MDL <MDL MDL <MDL MDL MDL <MDL <MDL <MDL <MDL MDL MDL MDL MDL <MDL MDL MDL MDL <MDL MDL <MDL <MDL MDL <MDL BACK TO MAIN Avene* MS afe MDL <MDL 3% 97% MDL <MDL MDL MDL <MDL <MDL MDL <MDL RSD Std. Dar. MDL MDL 00205 0103 MDL MDL <MDL <MDL <MDL MDL MDL MDL <MDL M 6 Cale. CMc. afe <MDL <MDL <MDL <MDL 267 314 272 241 OOO OOO OOO OOO OOO OOO ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo Tata! Awaaat TM afe <MDL <MDL <MDL <MDL 19% 105% 91% 11% <MDL <MDL <MDL <MDL <MDL . <MDL <MDL <MDL <UDL <MDL <MDL <MDL <MDL <MDL <MDL MDL *MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL n ooo ooo ooo oooooo oooooo ooo ooo ooo 0.00 ooo ooo ooo MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL Avertei MU MDL MDL 97% MDL MDL MDL MDL MDL MDL MDL MDL RSD . Dot. MDL MDL 0141 0123 MDL MDL MDL MDL MDL MDL Date Eetaad/Aaalyat: Date Vcrfleri/Aaalt: O W N , OVIM), 03/ 17/ , 03/22/9, OS/2 06/K /99, 06/09A9 EAD li- mo-foni MS13 C9FI9000* M6I3 CI fF23COO- FACT-M-2.0 Excel Vcnioa 7.0 T0X*l20*ttvrjJi tnwn 3;12fM ---- - FAU-w-*wC-l20 I 2S-Day O ral Toz Study by Dally G avagt la Rata Stady: hoAHhata(Te< Satomca): Mabod/Revtnaa: Analytical E q w p M S ^ X i a k r Dm* orExtnctfaarAadyec DMoTAnalysU/Aaatyal: Date of DaU Redoctioa/Aaaly*: Sample Data TOX*UOlExplontory2S-OayOralToskfeyStadybyDailyOsvajab Rati T-7023,T-7024,T-7022,T-7032,T-6293 RatLiver FDesame: FACT-M-I.OA FACT-M-1* R-5qacdVaia: Madefiac041091,Soap020199 Slope MamLyuU Y-bwccpC 04/19*9 RWW/3CWSAH 04/10/99,05/1499,05/25*9 H01/DRBT o v u m , a s rts m , o s n tm , o s n t m kjh/m ee/d rb/h q i See AlUdmeats RAT LIVER Craap Dow Method BUc 0 Matrix Blk 0 QC-2M R* qc-iM n * QC-250 ppb Cnap 1 0.0 mg/kg/ity Cottavi C raapl 0.0 mp/kg/day Recovery 1 - 14 Deyr G reapl OOa^/kg/day Recovery 2 -21 Dtyi G reapl 3A0avAUay Tekmer Alcohol Group! 30.0 mg/kg/dxy Recovery 1 -14 Day Groopl 30.0 mg/kf/diy Recovery 2 - 21 Day Graap3 30.0 mf/kfUay Tdomcr AcryiM Croap] , 30.0 mg/kg/day Recovay l - 14 Deyr GraapS 30.0mg/kg/dxy Recovery 2-2 1 Doys Sample i H20B&-1 H20 Blk-2 b S E D w lS T Rabbit liver Btt-2 3M-MS 3M-MSD 6M-MS 6M-MSD I3M-MS 13M-MSD IM 2M 3M 4M 5M 6M 7M IM 9M I0M DM 13M MM ISM 17M tIM 19M 20M 21M 22M 23M 24M 25M 26M 27M 29M 30M 3IM 33M 34M 35M 30M J7M JIM 39M 40M 41M 42M 43M 4SM 46M 47M Extracted Wf. E 0.00 0.00 0.00 0.00 0.9162 0.9162 0.9090 0.9090 1.0701 1.0701 oasi* 0.6999 0.9162 0.6126 0.1920 0.9091 0.0965 0.0267 0.6344 0.6972 0.0504 1.0701 0.9044 0.0511 1.1*03 0.9991 0l5039 Ol533I 07957 1.0663 0.7240 0.5990 05611 07356 09176 09513 . 1.0055 0.9514 06409 06715 05000 09225 05915 0.5744 07240 06670 1.0153 0.7J4J 0.9065 1.0667 09633 097I pros Calc. C ent a rt <MDL <MDL <MDL <MDL 274 206 319 300 209 291 170 129 102 124 104 130 127 165 115 339 312 94J 107 95.7 543 402 39.7 36.6 34.6 2SJ 19.2 29.0 32.9 26.0 25.5 44.9 93.0 31.7 70.4 30J 65.2 50.0 76.2 40.7 006 92.5 41.4 64.S 49.9 59.7 53.1 . 56.6 TatalAai--at afPFOS A <MDL <MDL <MDL <MDL 92H 96% 107% 103% 97% 97% 0170 0129 0102 0124 0104 0131 0127 0.165 0115 0339 0.3119 0094 0107 0096 00545 00402 0.0397 00366 00346 00259 00192 0.0290 0.0329 00260 00255 00449 00930 0.0317 00509 . 00652 /o o sto 00762 00417 00006 0.0925 0.0414 00645 00499 00597 00531 00566 Avene* pros a rt <MDL MDL 94% 105% 97% 0133 0255 0099 0.0950 00201 00560 00670 0.0SI9 00565 MethodDdectioaLink(MDL): nOS-UatfePOAA-Sapfe PFHS-05ag/g,M513-25ead50art-U613-25>rt DM Ealend/Aaaly*: D M V otfcd/A aaty: 0JA/99,05/26/99 LAC O0PC/99,06/0*99 BAD USD 8td.Dc*. <MDt <MDL 4% 4% 0.0 20 003 47. 01220 097 0.0069 306 0.0107 14.0 0.00416 57.6 0.03272 22.3 001515 ns 0.0117 5.10 000327 * POAA Cak. Cmk. a rt <MDL <MDL tDiVAM 9D1VAM . 254 260 299.93 310.30 344.99 312.71 OOO 0.00 0.00 OOO 0.00 0.00 OOO 0.00 0.00 000 0.00 OOO OOO 0.00 5077 3415 1366 7W0 4944 7045 5713 10045 4197 3712 2173 1519 1662 2026 6005 1032S 1091 1559 1463 10095 7591 10021 4711 1545 3764 1157 117 1731 Tarai Amaaat CPOAA a rt <MDL <MDL D1V/M DIVAN 5% 7% 100% 104% ' 115% 109% <MDL <MDL <MDL <MDL . <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MOL <MDL 5.01 3.49 037 73* 4.94 7.04 5.71 10.0 4J0 3.71 0*7 \S9 1.66 2.03 6.01 I0J 009 056 046 ion 7.59 10.0 4.7* US 3.76 1.16 O tt7 1.74 WA-Not Applicable .FF0S-PcrflnoraoctaacaBUbaM POAA" FeriiaorooctasoM FFHS PcrfliorohcxaaemlfcnM MS13 * C9F19COOM 6I3Cl1F23CDO* Average POAA a rt <MDL DIVAN MH 102% 110% DIVAN DIVAN fDtVAH 639 339 IM . 064 3M IM BACK TO MAIN USD Sid. P ot. <MDL DIVAN 2% 3% 010 DIVAN DIVAN DIVAN 317 2.1 10 0.669 103 0234 rc.i 14 402 MS 34J 0435 CMc. Case. ............ r t ______ NR NR NR NR VALUE! VALUEt VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! . VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! ' VALUE! VALUE! VALUEI VALUE! VALUE! VALUE! VALUE! Tarai Amaaat af * NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR Average a rt NR NR NR NR NR NR NR NR NR NR NR NR NR NR USD Sid. Dev. NR NR NR NR NR NR NR NR NR NR NR NR NR NR FACT-M-2 0 Excel Venfoa 7.0 TOX-I20-livcr.xle tnom 5:12 PM FAC-- -- --10 21-Day O ral Tox Study by Dally Gavage In R ati BACK TO MAIN Pndaet M-- twT Mhatatce); McAod/RcvUea: Aaalytkl E oslpnat MytUm H m k m hU nw ti S o ftw afw V ertio DattofExtnctW Auly: DU* o f Aaalyrit/AtiJyat: Dale of Dati Kidoctio^A--lyiC S ia p U Data TOX-120, Exploratory 2S-Dey Oral Toxidty Stady by DailyGavaga la Rats T-7023, T-7GM, 7-7022, T-7032. T-4295 K Liver SeeHattorighi PCT-M-1.0APACT44-2.0 k tad d H 04109, l o # 000199 Mnd.yUl.2 R-Sqend VibcSceAttachments --SN--C W IV 9 9 RWW/X7/SAH 04/2A99,03/1499,05/23/99 HOJ/DRBT 04/23/99,03/03/99,03/1199,05/2699 KJH/MEE/DRR/HOJ RAT LIVER ClM f Daaa Method B& 0 Matrix Bft 0 Q C -2 5 0 B * QC-U On* Q C -JM p1* C napl AOng/kffiday C iani Croopl 0.9 Agfiay Rocovwy t 14 D m Craof 1 AOo#kgMay Recovery 2 *21 Dtye Cmp 2 SAOng/kg/day Telarne* Alcohol Craap2 SAOngftfttay Rocovoy 1-14 D m Craapl M-Ong/koUay Racoray 2 *21 Dayi Craap J SAOng/kgMay T d n u r AoyiiM Groof 3 3A0 m t/kjK f Recovery 1 - 14 D m Gnop J SAOng/tytey Recom y22ID m -- ploi tilrtdaiW L H20BIM H20 BJk-2 RiMfl Liver Blk-1 . Rahbit Uvtr Bft-2 3M-MS 3M-MSD 6M-MS 6M-MSD 13M44S 13M-MSD IM 2M 3M 4M 5M CM 7M fM 9M 10M 11M 13M (4M 15M 17M ISM I9M 20M 2IM 22M 23M . 24M 23M 2CM 27M 29M 30M 3IM 33M 34M 33M 36M 37M 3IM 39M 4QM 41M 42M 43M 43M 46M 47M 0.00 0.00 0.00 0.00 0.9102 0.9102 0.9091 0.9091 1.0701 1.0701 0.451 S 0.4999 0.9142 a 6124 0.1920 0.909 0.1945 0.1247 0.4344 0.4972 0.1504 1.0701 0.9044 0.1511 . 0.9993 0.5139 0.5331 0.7957 1.0443 0.724 0.5990 0.541 0.7354 09174 A9S13 1.0435 0.9514 0.4419 0.47(5 0JII0 0.9225 0391) 0.5744 0i7240 0.4470 1.0153 0.7343 0.9065 1.0647 0.9633 0.9711 rn is Cale. Case, aa'c /MDL <MDL MDL MDL 273 254 319 311 247 291 49.0 94.4 49.7 50.9 SU 54.7 40lS 41.9 444 221 122 IM 90.4 419 34.4 454 SM 112.1 214 214 324 29* 34.9 14.9 22. 344 44.1 21.1 444 53.1 ai . 454 / 46.7 564 50.7 104 22.7 334 31.1 294 30.4 274 Method Detection U n * (MOL* P K - 2 J a * j . P O A A - 3 n f f c m t t TatalAnaaat rrn u _ gfr <MDL <MDL MDL MDL 91% 9% 107% 104% 9% 97% 0.0690 0.0966 0.0497 0.0509 0i0313 0.0547 0.040 O M I, 0.0442 0.221 a 122 0.10* 0.0904 0.0619. 0.0364 0.0455 0105S4 Ol 12 0.0254 0.0214 0.0320 0.0299 0.0349 A0I49 0.0221 0.0345 0.0441 0.0211 0.0643 0.0531 0.0901 0.0453 0.0667 0.0563 0.0507 0.1031 0.0227 010334 0.0311 0.029 0.0306 0.027 IMeEMcmd/Aaalyit: Data Verified'AaafyvC 05/05*9. 05/26/99 LAC 06W 99. 049999 BAD |i i MDL <MDL U% 105% 93% 0.0544 0.129 0.0190 0.0440 0.0255 0.0332 ao<43 0.0291 0.0293 R5D StADev. <MDL <MDL 7% 2% 0.0 374 0.0204 a . 0.0M4 21. 0.0194 424 0.0211 404 0.0103 34J 0.0114 JA9 0.0205 194 0.00559 5.42 0.00159 U iii CUc Cm c . Mt <MDL MDL <MDL <MDL 225 227 239.95 29340 217.43 267.66 aoo 0.00 0.00 ooo 0.00 0.00 0.00 0.00 0.00 A00 A00 A00 AOO 0.00 . 102 415 1615 2252 3155 2007 202! 2720 1491 2244 1443 1144 1222 1475 2304 2001 1727 1775 ISS) 1234 1619 1621 71 1174 1270 692 969 741 Total Am ont TM5I3 tfl MDL <MDL MDL <MDL 75% 76% 0% 9t% 73% 90% <MDL <MDL <MDL <MDL MDL <MDL <MDL <MDL <MDL <MDL <MDC <MDL MDL <MDL 1.03 A41 1.4 245 3.14 LOI L02 L72 1.49 244 144 144 142 1.47 240 2.00 1.73 1.77 1.SS 144 1.49 1.63 A79 1.17 147 A692 A949 A741 Avenge M913 rt <MDL <MDL 70% 9% 1% <MDL <MDL <MDL 1J1 1.73 142 1.71 l.ot A01 ftSS RAD*. MDL MDL 1% 20% A21 . <MDL MDL <MDL 44.0 AI7f 24.4 0.441 214 0423 174 407 23.7 0455 114 Al4i N/A - Not Apptieabla 9FOS90A A - Perfhorooctanoolo PFHS-9 M3U-C9P19COOM 6I3- CI1923000. M4U CalcCooc. tft MDL <MDL <MDL <MDL 241 229 117.04 21L60 225J1 233.71 AOO AOO A00 A00 A00 . AOO AOO AOO AOO AOO AOO AOO AOO AOO 9A4 494 ' 3.4 74.7 994 7L4 . 910 112 4L4 119 142 112 14 11 AOO AOO 424 AOO AOO AOO AOO AOO AOO AOO 0.00 AOO AOO AOO Tetti A tm at TM4U A <MDL MDL <MDL <MDL 11% 77% 43% 71% 76% 7% MDL MDL MDL <MDL <MDL MDL <MDL <MDL MDL <MDL <MDL MOL <MDL <MDL 0.0906 0.0693 A0S34 A0747 10995 A0724 A0960 A lt* A0614 Al 19 0.142 A113 A0464 A il MDL MDL A0622 MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL Average M41J A MDL MDL 79% 47% 77% RD StADev. MDL MDL 9% 12% 0.03 MDL MDL MDL MDL MDL MDL Aom A107 0.106 174 A0150 3J A04I4 144 A0172 A0632 MDL MDL N/A MDL MDL FCT-W-2.0 EiedVfw*7.0 T0X -1204W xk ifl/99 *12PM FAfcrr-rrra-120 1 28-Day O ral Tox Study by Dally Gavage In Rats BACK TO MAIN PwdeetNH*o(Tal Sebetnce): Muric Method/RcvUem Attlytfcal Eqwpaoi SystemNeater: Dm*orentacdoaMaabsl: Date ef Aaalynci'Aaatyit: DateofDau fedrtioaMaifyC Sample Data 70X420 Exploratory2f*DeyOnt TmxkkySlaty byDeflyQ m* faRett T-7OE3..T-7024, T-7Q22, T-7002T-629S Rat Liver FACT-M4.0ft FACT-M-2.0 Madeline04109S ' MamLyn3.2 04/19199 RWW/JCMAH 04/20*9,05/21/99. 05/25/99 HOI/DRB/KJH7 OSA2S/9R05/14/99, OS/2/99 MEE/KJH/HJ RAT LIVER CrM f Dm MctbodBtk Matrix Blk QC-JW ffk Q C -Jn* O C -!* C i |4 y Creep 4 0 -V ^T Recovery 1 14 Days Creep 4 Recovery 2-21 Dayt C rapf 1Q.0b | / | % PPHS Groo^S lOOmfgtfay Recovery 1-14 D m GreopS lOOaif/tfMay Recovery 2 - 21 Days Creeps pros Cnr l.SmkpMqr Recovery 1-1 4 Day* Creap 4 Recovery2*21 Days hqhl K20B&1 K20 BUt-2 Rabbit Liver Blk-1 Rabbit U r n Blk*2 3MMS 3M-MSD SM-MS 6M-MSD 13M-MS 13M-MSD 49M 50M SIM S2M S3M S4M SSM SAI S7M SSM S9M 4IM <2M 63M SSM SSM S7M SIM 69M TOM 71M 72M 73M 74M 7SM 77M 7IM 79M SIM S2M S3M S4M ISM MM 7M SSM STM 90M 9IM 93M 94M fSM pros Ceec. tfl 0 0 0 1 374.4500 314.0600 400.5000 3904100 379.4300 3S1.7SOO NR NR NR NR NR NR NR NR NR NR NR NR NR NR 21.7 73.0 NR NR NR NR NR NR 154 96.3 90.3 117 SS.4 S3J 246.9000 500.2700 3411500 429.1100 412.0600 419.7200 455.7600 403.1500 3219700 3304600 261.3200 209.1100 254.0100 251.4300 MethodDette* Urn*(MDI* FfOS-3ftJiffcFOAA-2$*fe, Ml 1. 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I 1 1 1 1 1 ! 1 ! 1 1 t 1 1 t 1 1 1 1 1 1 1 ! 1000 tooo 1000 1000 1000 . 1000 1000 (000 1000 tooo 1000 1000 1000 1000 pros Cak. Ceec tfl <MDL <MDL <MDL <MDL 27409% 21573% unni 30793% 21164% 29091% fVAL17EI VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUEI VAUJE1 VALUE! VALUE! VALUE! VALUE! VALUE! 21.5 7JJ VALUE! VALUE! VALUEI VALUE! VALUE! VALUE! 153 %! 90.6 117 S9.6 4.0 245S20 50271 . 352317 / ' 433347 47*539 4*549 456739 400571 J15IM.1 332S25 2700211 79017 253062.1 250517.1 Date EaUroVAealyat Date Vcrified/Aufyit: 02/26/99,03/10/99, 03/17/99,03/72m . 0*24/99,05/26*9 LAC w a rn , w w n bad Avrai pros <MDL <MDL 94% 105% 97% NR NR NR 0050 0.114 6.1051 419.4 309J 1312 USD M.DCV. <MDL <MDL 4% 4% 00 0.0 NR ao NR 0.0 NR S2 0.0317 303 0.0344 24.1 0.0261 20.44 15.73 1I.M 34.19 10.7 25.4 POAA Cale. Ceec. rato <MDL <MDL <MDL <MDL 254 260 299.93 310.30 344.99 312.71 VALUEI VALUE! VALUE! VALUEI VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! 0.00 0.00 VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! 0.00 0.00 VALUE! 0.00 0.00 0.00 VALUE! VALUE! VALUEI VALUE! VALUEI VALUE! VALUE! VALUE! VALUEI VALUEI VALUEI VALUEI VALUE! VALUE! Tetti A a u e P ef POAA er <MDL <MDL <MDL <MDL 15% 17% 100% 104% 115% 105% NR NR NR NR NR NR NR NR NR NR NR NR NR NR 0.00 aoo NR NR NR NR NR NR aoo aoo NR aoo 0.M aoo NR NR NR NR NR NR NR NR NR NR NR NR NR NR N/Aa NotApplicable FFOSPOAA-P HPHSPeritai Avente POAA rato <MDL <MDL 16% 102% 110% NR NR NR ao aoo aoo NR NR NR BSD SM. Dee. MDL MDL 0.0232 0.0340 ' 6-09*2 aoo NR aoo NR aoo NR HV/M aoo MV/M aoo SUV/M aoo aoo NR aoo NR aoo NR Crie. Ceec. rato VALUE! VALUE! VALUE! VALUE! VALUEI VALUE! VALUE! VALUE! VALUE! VALUE! *07 2433 2447 2703 *03 2034 4347 5162 16S 29.4 It! 25.0 405 103 VALUE! VALUE! VALUEI VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUEI VALUEI VALUEI VALUE! VALUE! VALUE! VALUE! VALUE! VALUEI VALUE! VALUE! VALUE! VALUE! rt NR NR NR NR NR NR NR NR NR NR 4.0 2.41 145 170 AIO) 101 4.35 5.SS ai6* 00294 OMI 00250 00405 00103 NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR Avente rato NR NR NR NR NR RSD ltd . Dee. NR NR NR NR NR 1.09 0126 00253 SU US ` 66.6 O0S4O 59.7 00151 0 NR NR aoo NR NR aoo NR NR aoo NR NR aoo NR NR aoo NR NR FPtArCrTl-VMe-n2t.o0*7.0 TOX*120-Uvtrjdi mom 5:12PM FACT-75X-120 ' ' *" 28-Day O ral Tox Study by Daily C iv a ie la Rate BACK TO MAIN Stady: frodati H a M T o t Sabataace): Mctbod/Rcvirioa: Aaalytfcal Eqriproc* Syrien N n h c r lanrarocai SoftwarriVcrrioa: D M ofE xlnctio^A ailytt . Date of AiulysiriAaalyri: Data of Dota RcdactioriAaaly: S ta p l* Data T0X'I2A BxplontfOX-13A Explantoiy 2S-Day Otri Toxkfcy Stady ly Daily Gavage la RM 'T-7Q23, T-7024, T-7D-7023, T-7024, T-7022, T-7032. T-629S . RatUvcr RatUvcr Stallai le righi FACT-M*1.0 A FtPACT-M-l.O A FACT-M-10 . K-Sqund ValaeSce AtuduneMs Maddfac 04109 Maddfae 041090 ManLynS.2 M rod.yn3J OVIW9 RWW0OM/1W9 AWW/7CMAH 04/3<m, 03/31/99, 0V299, 03/21/99,03/25/99 HOJ/DRB/KJH7 0V0V99,osa*m, osmn. osa*m, osaan mee/kjh/ho; RATUVER Craap MohodBlk Matrix Dlk 4 Q C -M O fi* Q C -U O jfk QC- 250ppb Creay 4 30.0atyday Grwty 4 30.0 m f t y i a y Recovcnr 1-14 D m Craap4 30.0W V d a y Recovery 2-2 Deys C rw yl 10.0ng/kfUay PPHS GreapS I 0 .0 m $ /k f/itoy Recovery 1-14 D an GraapS 10.0 r o n d a r R teo m y l-llb m Craap6 3.0 rog/fcf/day PPM Craap< S.Oafftfttay Raconuf 1-14 Dey* Greap6 S.OmtfktfAay Recovery 2-2 1 Dey S ta p k l H20 Blk-1 H20Blk-2 Rabbtl LJvcrBtk-t Rabbif Uver Blk-2 3M-MS 3M-MSD tM-MS tM-MSD 13M-MS 13M-MSD 49M 30M 31M . 92M 33M 54M 33M 56M 37M 3IM 39M 6IM 62M 63M 65M 66M 67M 6M <9M 70M 71M 72M 73M 74M 7SM 7714 7SM 79M IM 2M ISM 4M ISM 4M 7M IM 9M . 90M 91M 93M 94M 95M PFOS C ru. mtft 0 0 0 191 374.4500 314.0600 40QJOOQ 390.4400 379.4300 3 1.7300 NR NR NR NR NR NR NR NR NR NR NR NR NR NR 21.7 73. NR NR NR NR NR NR 154 913 90.3 117 14 13.3 2419000 30012700 3411500 429.1S00 4110600 419.7200 435.7600 403.3500 3219700 330.4600 2613200 209.1100 254.0100 251.4300 PPHS CafeCeac. rt <MDL <MDL <MOL <MDL 271 254 319 311 267.42 290J1 rVALUEl VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS xom 339107 213354 337636 34SB1 316441 223309 317000 69362 41369 67999 17903 ,, 42766 2(199 VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALU VALUS VALUS VALUS VALUS M e tr i D rotdaa m (M M PPM - S A 3 */$. fOAA - 25 g. f TaCalAaroaat afPFHS ata <MDL <MDL <MDL <MDL 91% 5% 107% 104% 9% 97% NR NR NR NR NR NR NR NR NR NR NR NR NR NR 360 359 213 331 346 316 223 317 69.4 414 61.0 37J 411 212 NR NR NR NR NR NR NR NX NR HR NR NR NR NR Average PPHS irt <MDL <MDL 11% 103% 93% NR NR NR 327 61.9 36J NR NR NR D M bttnd/A riy DateVarifledfAaalyri: 0U H *, 0S/1A99,03/17/99, 03/22/99,09 06W 99,06/09/99 EAD USD SU-Dev. <MDL <MDL 0.0723 0.0237 0.0030 NR NR NR 13. 310 19.0 11.7 20.4 7.42 NR NR NR M313 Cale. Cmk. a rt <MDL <MDL <MDL <MDL 225 227 273.16 334.12 217.13 267.66 VALUS VALUS VALUE! VALUS VALUE! VALUS VALUS VALUS VALUE! VALUS VALUE! VALUS VALUS VALUE! VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUE! VALUE! VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS TaUl Aaraaal ef MS11 Pi <MDL . <MDL <MDL <MDL 75% 7H 91% 112% 73% 90% NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR * NR NR NR NR NR NR NR NR NR NR NR NR NR NR Average MS13 __ * <MDL <MDL 70% 102% 11% NR NR NR NR NR NR NR NR NR N/A-NotAppeibla PFOS- OAA - rcrffcMieoctaaorie PFHS- BSD S U Dev; <MDC <MDL 1% 20% 011 NR NR NR NR NR NR NR NR NR M613 Cak. Caac. Pi <MDL <MDL <MDL <MDL 341 229 117 213 225.91 233.71 VALUS VALUE! VALUS VALUS VALUS VALUS VALUE! VALUS VALUS VALUS VALUS VALUS VALUS VALUE! VALUS VALUS VALUE! VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUE! VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS W alu i VALUS VALUS VALUS VALUS VALUS Tetri A m aat ' efM ltS a rt <MDL <MDL <MDL <MDL 1% 77% 63% 71% 76% 71% NR NR NR ' NR NR NR NR NR NR NR NR NR NR NR ' NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR NR Arrrage M613 rii <MDL <MDL 79% 67% 77% NR NR NR NR NR NR NR NR NR RSD StLDcv. MOL <MDL 9% 13% 0.03 NR NR NR NR NR NR NR NR NR FACT-M-2.0 Excel Vcnloa 7.0 TOX-120-tiverjs tnvn 3:12 PM 3M Environmental Lab - Fluorine Analytical Chemistry Team BACK TO MAIN PFS's PFHS perfluorohexanesulfonate C6F13S03- PFOS perfluorooctanesulfonate C8F17S03- PFOSA C8F17S02NH2 PFOSAA C8F17S02N ((CH2CH 3)(CH 2C00)> M 556 . C 8F17S02N ((H )(C H 2C 00))- M 570 C8F17S02N ((CH3)(CH 2C00))- PFO SEA perfluorooctanesulfonylethyl amide C8F17S02N ((CH2CH 3)(H)) . Et-FO SE-O H C 8F17 S 0 2 N ((C H 2C H 3)(C H 2C H 20H )) PFDS perfluorodecanesulfonate C 10F 21S O 3-** PFCA's POAA perfiuorooctanoate M 513 perfiuorodecanoate M 613 M463 perfiuorododecanoate M563 M363 C7F15COOC9F19COOC11F23COOC8F17COO- ** C10F21COO- ** C6F13COO- ** PFS = perfluorm ated sulfoante PFCA = perfluorm ated carboxylic acid ** no standard m aterial available fo r confirmation; 3M Confidential - A ttom ey/Client W ork Product Privileged - D o N ot D isclose BACK TO MAIN BACK TO MAIN 1 POAA in Male and Female Rat Liver, Group 2 1 3M Environmental Lab - Fluorine Analytical Chemistry 10 9 18 7 I 6 5 4 3 2 1 ]0 ] M513 in Male and Female R at Liver, Group 2 3M Environmental Lab - Fluorine Analytical Chemistry 1 Team, Project FACT-TOX120 ' 3 L 2] g 2.5 -- 2.5 c 0 21 i l 1-5 3 1~ 1 I 0.5 J0 " 1 1 0.5S -Female -Male 0 * 14 28 Day ] M613 in Male and Female Rat Liver, Group 2 3M Environmental Lab - Fluorine Analytical Chemistry ] 0.16 0.1' c o.i; 0 10.1 0.08 8I E t 0.06 O o 0.04 a S 0.02 0 Day BACK TO MAIN BACK TO MAIN TOX-120,35M G3 060899009 100-, % M6T3 (Cl lJb'2JCOO-)| 0.69^.75 0600889999009 ; 10 0 -, 1.72^2.13^ \ 2.58 3 \ 3 J 1 3.85 |M363 (ClUF21COO^ 3: MRM o f 1 Channel ES 5.85 TIC . 1.09e3 I 6.06 5.095 5o 5 . 6 5 1 / 6.89 7.71^7.86 / 869 6: MRM o f 1 Charmiel ES5.72 TIC 559 % J 038 54 06089900? 100 103 ' f i r 1-59 3f . M 3 " 7 M513 (C9FT9CO-)! 4-" ^ 2S^ k 6 L , U'JV 57027 \ 7-9S 8-4r 8'2> - 2: MRM of 1 le lE S - 5.55 TIC 8.68e3 %. 06089900?" 100-, 5.14 ' I i i t y r T'T r ,| ,,T i i r j i i p r i > i p r r i i | "i rl nl --1t--|1--iI--i f I r !--| j 4 1--r-- M463 (C8F17CO-1 536 i ii i i i ii i i111,"ri"i i i ii i i i i i i-i i11111 i i 5: MRM o f 1 Channel ES- TIC 6.65e3 %. J 060899004' " 100- 1 i 111111111 I1 1 1 1 I ''' ri I " 1 I 1 1 I 1 i` I 111 PUAA(CyElbUOai 5.14 I 1 " 1 I " I 1 1 1 1 m ` I 1 '1 !' ' '1 . _ _ 1: M RM o f 1 C hannel ESTIC 1.56e5 %- pi iij it rp'| ii i i j i iiij i^ r i|i i i ij ii i iIi i i i Ii i i i 06089900? M363 (C6ETJCOCEJI 0.66 1-08 1*64 2.23 17'! I II I II IIIl 1 1 I1 1 1 I1 1 1 1 IF"T I 111 ITI" 4: KRM o f 1 Channel ES TIC 489 Time BACK TO MAIN BACK TO MAIN .Envirnmental Labratory RequestForm ' BACK TO MAIN BACK TO MAIN !s Chain of Custody Record ; i v t n f : : 3 M Environmental Laboratory ' ; nQ7 iW ' UO /1 0 3M Requester_ C ...... . . .............. ... __________ ' M o r i / i n ___ ( f t K p _______________________ 3M ProjectLead Form 35178-PWO ' ' i : 1 ' ' :'' : *' ' ' '' '' 3M Facility ............. .. 3M ProjectTHIS , , .. ddF 3 fti T n y i r . o i o o a WTnv OW q t t ------ sj J ------------ ;------------------- 3M FacUllyContact ' ' -QZhia&isi' City,Stala,ZipCoda 1 bntj : r t ; ' tjii . :,:i! 3M TelephoneNumber' :; ^ `` 'k r s -IriinPri . ,-r- 1. 3M ProjectNumber . . ' > . ` ,.. . T K X \ 2 .TA,, I W l T D v Ppft i, *O ' PAjL/DQlfmvn / RlJtD G I Z A A A 3M DepartmentNumtier f. '' A /= 'Ai,1..? Ori? Pi i; ' LabRequasVTemplaleNumber ____ 1__ 5 ) . M M B i s m - . ______________7 3 3 - ,S l ) ? n SampledBy(Print) SamplerSignature . Item # 41 I 2 Cito 1 13 4 4 ~t v l - ................. ' h)lA ; - 1 ... S am ple Description Contractor,...,,, . .. X M iJfi !t-: . ' '!;. _____ A )q t o x __________ Date -ll/o 3 j n 3 / o `i/9 Date Tim e Matrix Num ber Containers ?, of each Preservatives J 8Vii ozX 8N X i' X i t i 0*! 1 * 1. . < L itlr.r Lii/tr 1 \rtr ,i i-:J* ! - W 'l , ** ' t ' J( 38 38 Total Containers of Each Item O Sam ples S plit See A ttachm ent : C om m ents `Gli/iiAVeP ' Vni-ko-ldrdE* 'u p n n " ' 5 r i m p i p ' rPTf'ini 5lP O 'l-Q fiv /J SpPri/nffn li V ' 4rir ' ' t 01 J{j O iXJiSW ' Ter v/r irvrtor^na 1v. v , i . :1 ". >....4 r r n u f ) ( j I ' i i i o ; 5.: ' ... . ... - ....... * . .. . ' p *lo ifa < i /' 1 5 Arj> 6 (irpb 7 8 L u rtr Ltirtr l, 38 MiOOu ih ilO lit./! 3 8 ;ifr . J0.f 'i ff f ' . : j il.i.rC;;: *, ' 9 ` *- ' * . **, ; * *' 10 11 12 Vf '- ' / * , t . f-.r- .; ' . r ; ;t 1 it.`< T fin c ia J ^ - -- . - -I . *_ 13 ----- ----------- -- ------- ------ --- --------;-------- ------------------------------------------------- ---- - item # Relinquished By/Affiliation Time Date Shipped Via Received By/Affiliation . . Tim e , ; Date .T r t Original - Accompanying Samples ' .V . Last Page * Oiignetor i ; ; .................... Airlht-n yprr.n ( y f u i k CJp^/lniU. r . i v n ;ii ' > | '! liO "i !f. 1 J . * ' ; * i *.( 1 ` f ` ' ' lo i' 7 -t 41` } r .` .*`'i - ' . - A 10a O H ; lAp/v. c a / n j / ^ .. / See Reverse Side For Instructions B A C K TO M AIN -- T ------ ~ ------ *------ - -- J 1------ ------- ------- ------ ------ - -- Chain of Custody Record 3 M Environmental Laboratory Forai 3S176-PWO 3M Facility nQ 7rfif Uy (XQ ' SMRftQUMltf 5,,MPrsjactL M n /n . C.CiS* oad. K rTi Haases 3M ProjectNumbor 3 / h Tnyr<Li5n><va Addio 0J WPT M O H X m T X l 2 0 Z tD a u r ltb x P t InRcs BWaqnooo / RtlW M iapcp 3M FacilityContact *J 3M DopaitraontNumbor City.Staio,ZipCoda M nvih C ase. 3M TolopbonoNumbor WMl Lob RoquoaVTamplalo Numbor ___________ - S I Paul, M B 551331____ ___________ ______________ ? 3 3 - 5 ) 8 o ___________________________ SomplodBy(Print) SomplorSlanatura Item # VA . VIA S am p le Description 1 Cirp I 2 Grp 3, 3 Grpl 4 f GraV _ / 5 Grp S 6 rbrolI r 7 Conlnclor k)hThV Data ll/o 3 i f*f - I l e m b i Date Time Matrix Lro^r Livtr 1j\rfr LiW Liirtr Liw,r -4,-- Number Containers of each Preservatives 2 n zo X 8 N X X Total Containers of Each Item Q Sam ples S plit See A ttachm ent C om m ents LnA `ih aI IJ 1 tn r /\l# /i l jhh ^Arnrslii tR trjj)\ S p L lr c jv .o l Spf lmen it'J-1- -irtr 2 8 *nr ktr irvrormantTK 28 (At n tnjoifoq as i n ----- ---------- ----- -----------------------T ! 8 9 1 1^-o]__________ 10 / *ir *f 11 12 13 -------- -- ------------ ------------- -- Item# l-L Relinquished By/Affiliation Time Date Shipped Via Received By/Affillation **u- AtrLme. BxprtJi /Vk A CIlv^ s -. t . : **' .( ; * ' f1*' ,* : ' -W - Iv t\ *' 1. <~.f! :> rl * *' '.?! it*:<r:*>' ''< ru <fl .'it'/' Ifh Time Date 04* 3pfh 0 2 / n i M ,r 11/09 '98 16:24 FAX 31736418543 N0T0X B.V. BACK TO MAIN uvo/Ui7 1. 1. ]. ' . . j. PROTOCOL 1 T. - " : EXPLORATORY 28-DAY ORAL TOXICITY STUDY ' WITH j TELOMER ALCOHOL. TELOMER ACRYLATE, PFHS AND PFOS (positive control) '. BY DAILY GAVAGE | IN THE RAT ' FOLLOWED BY A 14/28-DAY RECOVERY PERIOD J NOTOX Project 242933 NOTOX Substances 63484, 83493, 83902, 84456 and 84141 3M Test No. T-7023. T-7024, T-7022. T-7032, T-6295 - page 1 of 16 - 11/09 '98 16:24 FAX 31736418543 1 ______________ NOTOX B.V. BACK TO MAIN 0004/017 1 TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS NOTOX Project 242933 3M Test No. T-7023, T-7024 1 T-7022, T-7032, T-6295 PROTOCOL APPROVAL 1 ] STUDY DIRECTOR : 1 ] I- Ir. S.C.M. de Hoog Date: 11 . S Q p b Q r r y b 1 Q Q < 9 SPONSOR: Marvin T. Case, DVM, PhD Corporate Scientist 3M Toxicology Services . ^r J j ] ' D*t,: i?re Page 2 - 11/09 '93 13:23 FAX 31736413343 NOTOX B.V. 1 i TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS BACK TO MAIN 0003/017 NOTOX Project 242933 ] PREFACE Sponsor ] Study Monitor ] } Invoice Contact - 1. Testing Facility . 1 Study Director Pathology 3M Toxicology Services 3M Center, Bldg 220-2E-02 St. Paul. MN 55144-1000 USA Marvin T. Case, DVM, PhD 3M Toxicology Services 3M Center, Bldg 220-2E-02 Telephone (651)- 733-5180 Fax (651)- 733-1773 Barbara Nelson 3M Procurement Operations 3M Center, Bldg 224-1N-04 St. Paul, MN 55144-1000 Telephone (651)- 733-0766 Fax (651)- 733-1799 NOTOX B.V. Hambakenweterlng 3 5231 DD 's-Hertogenboseh The Netherlands Xr. S.C.M. de Hoog Dr. J. Th. Wilson 4 - Page 3 - 11/09 *93 16:25 FAX 31736413543 1------------------------ N0T0X B.V. TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS BACK TO MAIN 008/017 NOTOX Project 242933 STUDY PLAN ] Start of Treatment 06 October 1998 (males) ] 08 October 1998 (females) Start Recovery 04 November 1998 (males) 1 06 November 1998 (females) I Blood sampling 03 November 1998 (Main groups, males) 04 November 1998 (Main and Recovery groups 1 and 2, males) 05 November 1996 (Main groups, females) 06 November 1998 (Main and Recovery groups 1 and 2, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December 1998 (Recovery groups 2, females) Urine collection predose 29-30 September 1998 (Recovery groups 1 and 2) day 7 11-12 October 1998 (Recovery groups 1 and 2, males) day 7 13-14 October 1998 (Recovery groups 1 and 2, females) J day 14 day 14 end of treatment 18-19 October 1998 (Recovery groups 1 and 2, males) 20-21 October 1998 (Recovery groups 1 and 2, females) 03-04 November 1998 (Recovery groups 1 and 2, males) end of treatment 05-06 November 1998 (Recovery groups 1 and 2, females) end of recovery 17-18 November 1998 (Recovery groups l, males) end of recovery 19-20 November 1998 (Recovery groups 1, females) ] end of recovery end of recovery 01-02 December 1998 (Recovery groups 2, males) 03-04 December 1998 (Recovery grdups 2, females) Necropsy ] 03 November 1998 (Main groups, males) 04 November 1998 (Main groups, males) 05 November 1998 (Main groups, females) 06 November 1998 (Main groups, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December 1998 (Recovery groups 2, females) Draft Report J 26 March 1999 Page 4 - 11/09 '98 16:25 FAX 31736418543 l NOTOX B.V. BACK TO MAIN 0007/017 TELOMER ALCOHOL/TELOtCR ACRYLATE/ /PFHS/PFOS ]. NOTOX Project 242933 TEST SUBSTANCES The sponsor 1s responsible for the completeness of all test substance data. All available Information on characteristics of the test substance will be taken Into account before start of the study 1n order to determine 1f they conflict w1th/m1ght affect the proceeding of the study. NOTOX SUBSTANCE 83484 (data will be added by protocol amendment) Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date Stability 1n vehicle Safety precautions Disposal Category TELOMER ALCOHOL (T-7023) Stable 1% aqueous carboxymethyl cellulose: not Indicated Corn oil: not indicated Gloves, goggles and face mask to ensure personnel health and safety NOTOX SUBSTANCE 83493 (data will be added by protocol amendment) 1 Identification Description TELOMER ACRYLATE (T-7024) Batch J Purity Test substance storage Stability under storage conditions Stable Expiry date ] Stability 1n vehicle 1% aqueous carboxymethyl cellulose: not indicated Corn oil: not Indicated -x Safety precautions Gloves, goggles and face mask to ensure personnel health and safety ] Disposal Category i - Page 5 - 11/09 -98 18:28 Fa x 31738418543 L _ _________ NOTOX B.V. 1 , TELOMER ALCOHOL/TELOMER ACRYLATE/' /PFHS/PfOS BACK TO MAIN @008/017 ' NOTOX Project 242933 NOTOX SUBSTANCE 83502 (data will be added by protocol amendment) 1 Identification Description (T-7022) Batch Purity Test substance storage ] Stability under storage conditions Stable Expiry date Stability 1n vehicle 1% aqueous carboxymethyl cellulose: not indicated 1 Safety precautions Disposal Category Corn oil: not Indicated Gloves, goggles and face mask to ensure personnel health and safety NOTOX SUBSTANCE 84456 (data will be added by protocol amendment) Identification PFHS (T-7032) Description Batch Purity Test substance storage Stability under storage conditions Stable Expiry date Stability 1n vehicle ' 1% aqueous carboxymethyl cellulose: not Indicated J Safety precautions Disposal Category Corn oil: not Indicated A Gloves, goggles and face mask to ensure personnel health and safety NOTOX SUBSTANCE 84141 (data will be added by protocol amendment) ] Identification PFOS (T-6295) Description ' Batch - ] Purity Test substance storage Stability under storage conditions Stable Expiry date Stability In vehicle 1% aqueous carboxymethyl cellulose: not Indicated Corn oil: not Indicated Safety precautions Gloves, goggles and face mask to ensure personnel health and safety Disposal Category ] Vehicle 1% aqueous carboxymethyl cellulose or Corn oil, specific gravity 0.92 Rationale for vehicle Based on trial formulations performed at NOTOX Stability 1n vehicle Stability over 4 hours will be determined by the * sponsor - Page 6 - 11/09 '93 16:26 FAX 31736413543 NOTOX B.V. BACK TO MAIN 009/017 TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS . NOTOX Project 242933 TEST SUBSTANCE FORMULATION Method Storage conditions Formulations (w/w) will be prepared dally within 4 hours prior to dosing. If necessary, adjustment will be made for specific gravity of vehicle. At ambient temperature No chemical analysis of dose preparations will be performed. PURPOSE AND RATIONALE The nature and purpose of this exploratory study will be to determine and compare the relative oral toxicity in rats following oral (gastric gavage) doses administered daily over 28 days and Identify developmental compounds of lesser toxicity for commercial development. This study should provide part of a rational basis for toxicological risk assessment 1n man. The oral route was selected as it Is a possible route of human exposure during manufacture, handling or use of the test substance. GUIDELINES This protocol was reviewed and agreed by the Article 14-functionary and the Ethical Committee of NOTOX as required by the Dutch Act on Animal Experimentation. The study procedures described 1n this protocol will be based on the following guidelines: . 1) EC Directive 96/54/EEC, Annex IV.D, replacing EEC Directive 67/54B/EEC, Part B : Methods for the Determination of Toxicity and other'Health Effects; B.7: 'Repeated Dose (28 days) Toxicity (oral)*. Official Journal of the European Communities No. L248, September 1996. ' 2) OECD Guideline-for Testing of Chemicals, Guideline 407: 'Repeated Dose 28>day Oral Toxicity Study 1n Rodents*, Paris Cedex, 27 July 1995 This protocol, study procedures and the report will not be Inspected by the NOTOX Quality Assurance Unit. ARCHIVING NOTOX B.V.. will archive the following data for at least 10 years: protocol, raw data, report and all specimens. No data will be withdrawn without the sponsor's written consent. - Page 7 - ll/Ofl '93 18:27 FAX 31738418543 I______ :_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ NOTOX B.V. 1 TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS ] BACK TO MAIN 0010/017 NOTOX Project 242933 TEST SYSTEM ] Test System ] Sprague Dawley rat CrV.CD BR, outbred, SPF quality. Recognised by International guidelines as the recommended test system (e.g. EPA, FDA, OECD, EEC). Females will be nulllparous and non-pregnant. Source : Charles R1yer, Sulzfeld, Germany Age at Start of Treatment Approximately 6 weeks 1 Number of animals Randomisation 84 males. 84 females At least 5 days before study start, by computer generated random algorithm according to body weight, with all animals within 20% of the sex mean. A health Inspection will be performed prior to commencement of treatment to ensure that the animals are 1n a good state of health. Identification Earmark and tattoo ALLOCATION Group Identification Test Substance Number of animals Animal numbers males females males females 1 Main group 1 Recovery group i 1 Recovery group 2 2 Main group 2 Recovery group 1 2 Recovery group 2 3 Main group 3 Recovery group 1 3 Recovery.group 2 4 Main group 4 Recovery group 1 4 Recovery group 2 5 Main group 5 Recovery group 1 5 Recovery group 2 6 Main group 6 Recovery group 1 6 Recovery group 2 Vehicle control Vehicle control Vehicle control Telomer Alcohol Telomer Alcohol Telomer Alcohol Telomer Acrylate Telomer Acrylate Telomer Acrylate PFHS PFHS. PFHS PFOS (positive control) PFOS (positive control) PFOS (positive control) 8 3 3 8 3 3 8 3 3 8 3 3 8 3 3 8 3 3 8 3 3 8 3 3 .8 3 3 8 3 3 8 3 3 8 3 3 1- 8 ! 97-104 9-11 105-107 13-15 109-111 17-24 113-120 25-27 121-123 29-31 125-127 33-40 129-136 41-43 137-139 45-47 141-143 49-56 145-152 37-59 153-155 61-63 157-139 65-72 161-168 73-73 169-171 77-79 173-175 81-88 177-184 89-91 185-187 93-95 189-191 i Test substances Telomer Alcohol (substance 83484), Telomer Acrylate (substance j 83493) and (substance 83502) to be tested at a dose level of 30 mg/kg body J we1ght/day. I PFHS (substance 84456) to be tested at a dose level of 10 mg/kg body weight/day. PFOS (substance 84141) to be tested at a dose level of 3 mg/kg body weight/day. I - Page 8 - \ 11/09 '98 18:27 FAX 31738413543 1 NOTOX B.V. TELOMER ALCOHOL/TELOffiR ACRYLATE/ /PFHS/PFOS BACK TO MAIN 0011/017 NOTOX Project 242933 ANIMAL HUSBANDRY Conditions Air-conditioned room with approximately 15 air changes per hour and controlled environment with optimal conditions being a temperature of 21 3*C and a relative humidity of 30-70%. Lighting will be a 12 hours artificial fluorescent light and t 12 hours dark per day. Accommodation Group housing of 3-4 animals per sex per cage 1n stainless steel suspended cages with wire mesh floors. Acclimatisation period Is at least 5 days before start of treatment under laboratory conditions. . Diet . Free access to standard pelleted laboratory animal diet (from Carfll Quality BVBA,.Oud-Turnhout, Belgium). Each batch will be analysed for nutrients and contaminants are analysed on a regular basis. Results are examined and archived. Water Free access to tap-water. Certificates of analysis (performed quarterly) are examined and archived. ' TREATMENT ! | Method I Frequency | Dose volume | Dose level 1I * j RECOVERY Oral gavage, using a stainless steel stomach tube. Formulations will be placed on a magnetic stirrer ' ' during dosing. 3 Once dally for at least 28 days, approximately the same time each day, 7 days per week. Main group animals are dosed up to the day prior to necropsy. . 5 ml/kg body weight. Actual dose volumes will be calculated weekly according to the latest body weight. .. Group 1: 0 mg/kg b.w./day (Vehicle Control) Group 2: 30 mg/kg b.w./day (Telomer Alcohol) Group 3: 30 mg/kg b.w./day (Telomer Acrylate) Group 4: 30 mg/kg b.w./day ' Group 5: 10 mg/kg b.w./day (PFHS) Group 6: 3 mg/kg b.w./day (PFOS, positive control) Duration Recovery group l: at least 14 days Recovery group 2: at least 28 days t \ - Page 9 - 11/09 '93 1:2S FAX 31738418543 l NOTOX B.V. 1 . TEIOMER ALCOHOUTELOMER ACRYLATE/ /PFHS/PFOS BACK TO MAIN 0012/017 NOTOX Project 242933 OBSERVATIONS 1 Mortality / Viability 1 Clinical signs 1 Body Weights ] Food consumption Water consumption Twice dally. Animals showing pain, distress or discomfort, which 1s considered not transient in nature or is likely to become more severe, will be sacrificed for humane reasons. The time of death will be recorded as precisely as possible. Twice dally, detailed clinical observations will be made 1n all animals: once Immediately after dosing and once approximately 4 hours after dosing. The time of onset, degree and duration will be recorded. .' All symptoms will be recorded and graded according to fixed scales (specified in the report). Treatment period : on days 1, 5. 8, 12, 15, 19, 22, 25 and 28. Recovery period : on days l, 5, 8, 12, 14, 19, 22, 25 and 28. Weekly. . Subjective appraisal will be maintained during the study and a quantitative assessment Introduced 1n the event of a suspected treatment related effect. CLINICAL LABORATORY INVESTIGATIONS (Main and Recovery group animals) ? J Blood samples will be collected under light ether anaesthesia, between 7.30 and 9.30 a.m. Immediately prior to post mortem examination for Main and Recovery group animals, and for Recovery group animals also at the end of the treatment 1 period. The animals will be fasted overnight (with a maximum of 20 hours) before blood sampling, but water will be provided. Blood samples will be drawn from the retro-orbital sinus of all Main and Recovery rats/sex/group and collected Into J tubes prepared with EDTA for haematologlcal parameters (0.6 ml), with citrate for clotting tests (1.0 ml) and untreated tubes for clinical biochemistry parameters (>2.0 ml). i - Page 10 - 11/09 '98 16:28 F A X 31736418543 NOTOX B.V. BACK TO MAIN 0013/017 i _______________________________________________- __________________________________: I ' TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS MOTOX Project 242933 ! HAEMATOLOGY The following haematology parameters will be determined from blood prepared with EDTA as an ant1-coagulant, using the Instruments listed: Parameter Unit Instrumentation J Erythrocyte count T/l ] Haemoglobin Haematocrlt mmol/1 1/1 . Mean corpuscular 1 volume fl iJ 1!1 . Mean corpuscular haemoglobin . Mean corpuscular haemoglobin concen tration Platelet count fmol mmol/1 G/1 11 JI Red cell distribution width % Total leucocyte count G/1 Differential leucocyte l(rel) count Sysmex K-1000 Sysmex K-looo 'Sysmex K-1000 Sysmex K-1000. Sysmex K-1000 Sysmex K-1000 . . Sysmex K-1000 Sysmex K-1000 Sysmex K-1000 Manual (Microscope) ^ 1 The following haematology parameters will be determined.from blood prepared with citrate as an ant1-coagulant, using the Instruments Hstfed: Parameter ] Unit Instrumentation Prothrombin time sec J Partial thromboplastin time sec Sysmex CA-5000 Sysmex CA-5000 . - Page 11 - 1 1 / 0 9 ' 9 8 1 6 : 2 9 FAX 3 1 7 3 8 4 1 8 3 4 3 NOTOXB.V. BACK TO MAIN _____________ _____ 1 ______________ _____________________ -- ___________________________________ telomer alcohol/t e l o m -R ACRYLATE/ /PFHS/PFOS 1 NTOX Project 242933 CLINICAL BIOCHEMISTRY The following clinical biochemistry parameters will be determined from serum samples using the Instruments listed: Parameter Unit Instrumentation J Alanine am1notrans> jikat/1 ferase 1 Aspartate amlnotrans* jikat/1 1 ferase , Bilirubin, total pmol/1 1 Cholesterol, total mmol/I Triglycerides j Creatinine mmol/1 |imo1/1 Glucose mmol/1 Urea . mmol/1 Protein, total . Q/1 j . Protein, albumin g/1 Protein, globulin g/1 J Albumin Globulin ratio l Alkaline phosphatase (ikat/1 j Sodium mmol/1 Potassium ' mmol/1 J Chloride mmol/1 Calcium mmol/1 | Phosphorus mmol/1 Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Epos selective analyser 5060 (Eppendorf) Calculation (Protein total - Albumin) Calculation [Albumln/Qlobul1n] Epos selective analyser 5060 (Eppendorf) EFIX 5055 Flame Photometer ,\ (Eppendorf). EFIX 5055 Flame Photometer (Eppendorf) Jenway PCLM3 EFIX 5055 Flame Photometer (Eppendorf) Epos selective analyser 5060 (Eppendorf) t - Page 12 - 11/09 '93 16:29 F1 01736413543 NOTO! B.V. BACK TO MAIN 1015/017 TELOMER AlCOHOl/TELOMER ACRYLATE/ /PFHS/PFOS NOTOX Project 242933 URINALYSIS (Recovery group animals only) Urine samples will be collected overnight (approximately 16 hrs) from all animals assigned to the Recovery groups according to the following schedule: - predose - during the treatment period on days 7, 14 and at the end of treatment at the end of the recovery period, prior to scheduled necropsy. During the sampling period animals will be deprived of food but water will be provided. Urine will be collected into a specimen vial, using a metabolism cage. After collection the samples will be frozen and stored at -20*C at NOTOX until further analysis. . . PATHOLOGY NECROPSY . All animals surviving to the end of the observation period and all moribund animals will be deeply anaesthetised using ether vapour and subsequently exsanguinated. All animals will be necropsled and descriptions of all macroscopic abnormalities will be recorded. Prior to exsangulnation a blood sample (1*2 ml) for serum collection will be taken from the aorta. Serum samples will be stored at -20*C at NOTOX until further analysis. ' Samples of the following tissues and organs will be collected from alif animals at necropsy and fixed 1n neutral phosphate buffered 4% formaldehyde solution: Identification marks Adrenal glands Aorta Brain ' Caecum Colon Duodenum ' Epididymides Eyes with optic nerve and Harder1an gland Female mammary gland area Heart Ileum Jejunum Kidneys Liver Lung, Infused with formalin Lvmoh nodes mandibular, mesenteric Oesophagus Ovaries Pancreas . " ' - Page 13 - 11/09 '98 18:30 FAX 31738418343 1-- - - - - - - - - - - - - - - NOTOX B.V. BACK TO MAIN 0018/017 TELOMER AlCOHOL/TELOMER ACRYLATE/ /PfHS/PFOS NOTOX Project 242933 II Peyer'* patches (jejunum, ileum) if detectable Pituitary gland ] Prostate gland Rectum Salivary glands - mandibular Sciatic nerve '' ] Seminal vesicles Spinal cord -cervical, mldthoradc, lumbar . Spleen Sternum with bone marrow Stomach ] Testes Thvmus Thyroid Including parathyroid Trachea Urinary bladder ' Uterus. All gross lesions ORGAN WEIGHTS The following organ weights (and terminal body weight) will be recorded from the surviving animals on the scheduled day of necropsy: Adrenal glands j Brain Heart Kidneys Liver J Ovaries Spleen Testes .,, . _ * . H . J Both absolute and relative (% body weight) organ weight data will be Included 1n the study report. . LIVER SAMPLING ] From all animals surviving until the scheduled necropsy at least 1 gram of the left lateral liver lobe will be frozen in isopentane/N*. Liver samples will be i stored at -20*C at NOTOX until further analysis. I I 1 Jj - Page 14 - 11/09 '9 16:30 FAI 31736416543 NOTOX B.V. 1- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - _ _ BACK TO MAIN ------------------ : I j TELONER ALC0H0L/TE10MER ACRYLATE/ /PFHS/PFOS NOTOX Project 242933 1. J HISTOTECHNOLOGY A ll organ and tissue samples, as defined under Histopathology (following), w ill ] be processed, embedded and cut at a thickness of 2-4 micrometers and stained with haeraatoxylin and eosln. Special stains w ill be used at the discretion of the pathologist. HISTOPATHOLOGY Slides of a ll underlined organs and tissues (see under Necropsy) collected at the scheduled sacrifice from a ll animals, as well as-from a ll animals of a ll dose groups which died spontaneously or were terminated in extremis, and a ll gross lesions of a ll animals (a ll dose groups) w ill be examined by a pathologist. A ll abnormalities w ill be described and included in the report. I STATISTICAL ANALYSIS i ------------------------------- The following sta tis tica l methods w ill be used to analyse the data: I f the variables can be assumed to follow a normal distribution, the Dunnetttest (many-to-one t-te s t) based on a pooled variance estimate w ill be applied fo r the comparison of the treated groups and the control groups for each sex. The S teel-test (many-to-one rank test) w ill be applied Instead of the Dunnett test 1f the data can not be assumed to follow a normal distribution. A ll tests w ill be two-sided and 1n a ll cases p < 0.05 w ill be accepted as the lowest level of significance. Additional methods of statistical analysis may be used at the discretion of the Study Director. The methods and the results w ill be described in the report. References: - C.W. Dunnett A Multiple Comparison Procedure for Comparing Several Treatments with a Control, J. Araer. Stat. Assoc. 50, 1096-1121 (1955). * j - R.G. M iller Simultaneous S tatistical Inference, Springer Verlag, New York (1981). i BACK TO MAIN 1I 1 i Page 15 - 1 3 ----- :- - - - - - - - - - - - - - - - - ' - - - - - - - - - - - r 1 TELOMER ALCOHOL/TELOHER ACRYLATE/ /PFHS/PFOS 1 DISTRIBUTION This protocol w ill be distributed as follows: J Original: 1 Copy: J 1 Copy: 1 Copy: 1 Copy: 1 Copy: 1 Copy: Study Director Coordinating Blotechnldan Clinical Pathology Necrop$y/H1stotechn1que Hlstopathology Management Sponsor NOTOXProject 242933 'f V' STF HIS 325/03 BACK TO MAIN N M otox Safety & Environm ental Research B.V. SPECIMEN DISPATCH LIST NOTOX PROJECT: _242933_ SPECIES: RAT TEST SUBSTANCE: 83484, 83494, 83502, 84456, 84141 SIGNATURE/DATE STUDY DIRECTOR 8 dQn-0^ 1999 TO: Name and adress company: 3 M Environmental Technology and Safety Services Attn. Kris Hansen 935 Bush Avenue Building 2-3E-09 . USA Ptci' tei-flS |H8o C k t m o a e o J es te l. 001 6 3 1 7 3 S 1 8 0 C n o ru n T . C o.se)og cov CPa-uX. L e x t e r ) Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Animal numbers males 1-li + 13-15 17-27 + 33-43 + 49-59 + 65-75 + 81-91 + 29-31 45-47 61-63 77-79 93-95 Animal numbers females 97-107 + 113-123+ 129-139+ 145-155+ 161-171+ 177-187 + 109-111 125-127 141-143 157-159 173-175 189-191 Number of liver samples 28 28 28 28 28 * 28 Also enclosed are : [ ] Copy of Protocol/Amendments [ ] Copies of histology raw data [ ] Study results [ ] Necropsy data transfer file C] . DISPATCH: Signature: Date:. O ... PLEASE SIGN AND RETURN THIS FORM AS SOON AS POSSIBLE AFTER RECEIPT: COMPANY/SIGNATURE. C V i.A . .CwH^.. . . ?/*l. Date:... ^ o ? l f . M . BACK TO MAIN LisaA. Clemen ^ 02/06/99 04:20 PM Environmental Technology &Safety Services ' (651)778-5568 3M Building 02-3E-09 PO Box 3331 S t Paul, MN 55133-3331 Fax: (651) 778-6176 To: cc: Subject Kris J. Hansen/US-Corporate/3M/US@3M-Corporate Karl J. Rambo/US-Corporate/3M/US@3M-Corporate Grant A. PaulAJS-Corporale/3IWUS@3M-Corporate ` NOTOX 242933 Sample Receipt . - On 02/03/99 we received 168 rat liversfromNOTOX 242933 (TOX120, W1220). These sampleswere receivedfrozenondry iceand have beenstored inFreezer 9 at -20 +V10C. * Lisa SAMPLES 4 \/BRlFteC> G f a f a f a . S' 3M Environmental Technology and Safety Services attn. Kris Hansen 935 Bush Avenue Building 2-3E-09 . St. Paul, MN 55133-3331 USA BACK TO MAIN H^NOTOX Hambakenwetering 3 RO. Box 3476 5203 DL 's-Hertogenbosch The Netherlands Tel.:(073)641 95 75 Fax: (073) 641 85 43 Date : 29-0AN-99 Ref. ; KiM Subject: NOTOX Test Substance No. 83484, 83494, 83502, 84456, 84141 Dear Kris, On request of Mrs. S. de Hoog herewith I send the Uver samples (total 168) from NOTOX project 242933. With kind regards, NOTOX B.V. Mariette de Klnkelder Test Substance Officer enclosure: a.m. Liver samples Chamber o f Commerce VHertogenboseh nr. 16055281 BACK TO MAIN NOTOX Project #242933 Feb. 4,1999 The liver sample numbers listed below did not include a collection date on their containers. After reading the protocol, I wrote the collection date on each container. See below for dates. If these collection dates are not correct, please call me at 1-165-778-5568. Regards, Lisa A. Clemen Ac Samples 1 2 3 4 17 18 19 20 33 34 35 36 49 50 51 52 65 66 67 68 81 82 83 84 Group 1 1 1 1 2 2 2 2 3 3 3 3 4 '4 4 4 5 5 5 5 6 6 6 6 Collection Date Added ? 11/03/98 711/03/98 711/03/98 . 711/03/98 7 11/03/98. 711/03/98 711/03/98 ? 11/03/98 7 11/03/98 ? 11/03/98 711/03/98 ? 11/03/98 ? 11/03/98 ? 11/03/98 711/03/98 ? 11/03/98 ? 11/03/98 711/03/98 711/03/98 7 11/03/98 711/03/98 711/03/98 7 11/03/98 711/03/98 Samples 109 110 111 125 126 127 141 142 143 157 158 159 173 174 175 189 190 191 Group 1 1 1 2 2 2 3 3 3 .4 4 4 5 5 5 6 6 6 Collection Date Added ? 12/04/98 ? 12/04/98 ? 12/04/98 7 12/04/98 ' 7 12/04/98 7 12/04/98 . ? 12/04/98 7 12/04/98 ? 12/04/98 7 12/04/98 7 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98 NOTOX #242933 TOX120 Liver Samples Received 02/03/99 BACK TO MAIN ' Samples Group Collection Date *1 1 11/03/98 '2 1 11/03/98 '3 1 11/03/98 ' 4 1 11/03/98 '5 1 11/04/98 *-6 1 11/04/98 '7 1 11/04/98 '8 1 11/04/98 *9 1 11/18/98 '10 1 11/18/98 'l l 1 11/18/98 S3 1 12/02/98 '1 4 1 12/02/98 '1 5 1 12/02/98 <7 2 11/03/98 -18 2 11/03/98 '1 9 2 11/03/98 '20 2 11/03/98 '2 1 2 <22 2 <23 2 11/04/98 11/04/98 11/04/98 '2 4 2 11/04/98 '2 5 . 2 11/18/98 '2 6 2 <11 2 <2.9 2 11/18/98 11/18/98 12/02/98 <30 2 12/02/98 '3 1 2 12/02/98 '3 3 .3 11/03/98 "34 3 11/03/98 <35 3 11/03/98 -36 3 31 3 11/03/98 11/04/98 '3 8 3 <39 3 11/04/98 11/04/98 '4 0 3 11/04/98 '4 1 3 11/18/98 '4 2 3 11/18/98 '4 3 3 11/18/98 \45 3 <16 3 12/02/98 12/02/98 v47 3 ' 12/02/98 v49 4 11/03/98 '5 0 4 11/03/98 '5 1 , 4 11/03/98 '5 2 4 11/03/98 <53 4 11/04/98 N54 4 11/04/98 '5 5 4 11/04/98 '5 6 4 11/04/98 '5 7 4 11/18/98 *58 4 11/18/98 <9 4 11/18/98 v61 4 12/02/98 <52 4 12/02/98 '6 3 4 12/02/98 Samples Group Collection Date -65 5 11/03/98 ,,66 5 11/03/98 vfi7 5 11/03/98 '6 8 5 11/03/98 '6 9 5 11/04/98 '7 0 5 11/04/98 '7 1 s 5 <72 5 11/04/98 11/04/98 '7 3 5 11/18/98 '7 4 5 11/18/98 *75 5 '7 7 5 11/18/98 12/02/98 *78 5 . 12/02/98 "79 5 ' 12/02/98 '81 6 11/03/98 ^82 . 6 11/03/98 '8 3 6 11/03/98 -84 6 11/03/98 ^ 5 6 11/04/98 -86 6 11/04/98 'i l 6 11/04/98 -88 6 11/04/98 *89 6 11/18/98 -90 6 11/18/98 ^ 1 6 11/18/98 *-93 6 '9 4 6 12/02/98 12/02/98 '9 5 6 12/02/98 ^97 1 11/05/98 '9 8 1 11/05/98 '9 9 1 11/05/98 <00 1 11/05/98 J 01 1 11/06/98 '102 1 11/06/98 '103 1 \104 1 11/06/98 11/06/98 '1 0 5 1 11/20/98 n J06 1 11/20/98 '1 0 7 1 11/20/98 <09 1 12/04/98 '110 1 12/04/98 <11 1 12/04/98 -113 2 11/05/98 <14 2 11/05/98 115 2 11/05/98 <16 2 11/05/98 '1 1 7 2 11/06/98 '1 1 8 2 1 11/06/98 <19 2 11/06/98 '120 2 11/06/98 '121 2 11/20/98 '12 2 2 11/20/98 '1 2 3 2 11/20/98 '125 2 12/04/98 M26 2 12/04/98 '1 2 7 2 12/04/98 Samples Group Collection Date M 29 3 11/05/98 -130 3 11/05/98 '131 3 11/05/98 -132 3 11/05/98 -133 3 11/06/98 '1 3 4 3 11/06/98 '1 3 5 3 11/06/98 <36 3 11/06/98 <37 3 11/20/98 '1 3 8 3 11/20/98 <39 3 11/20/98 '141 3 12/04/98 < 4 2 3 12/04/98 '143 3 12/04/98 '1 4 5 4 11/05/98 '1 4 6 4 11/05/98 -147 4 11/05/98 *448 4 11/05/98 <49 4 11/06/98 <50 4 11/06/98 -151 4 11/06/98 -152 4 11/06/98 M53 4 11/20/98 . <54 4 11/20/98 M 55 4 11/20/98 '1 5 7 4 12/04/98 <58 4 12/04/98 -159 4 12/04/98 <61 5 11/05/98 M 62 5 11/05/98 <63 5 11/05/98 -164 5 11/05/98 '165 5 11/06/98 '1 6 6 5 fl/06/98 '1 6 7 5 11/06/98 '1 6 8 5 ' 11/06/98 <69 5 11/20/98 -170 5 11/20/98 <71 5 11/20/98 * '< 7 3 5 12/04/98 <74 5 12/04/98 <75 5 ' 12/04/98 '177 6 11/05/98 <78 6 11/05/98 '1 7 9 6 11/05/98 -180 6 11/05/98 <81 6 11/06/98 <82 6 11/06/98 '183 6 11/06/98 n184 6 11/06/98 J85 6 11/20/98 . <86 6 11/20/98 <87 6 11/20/98 <89 6 12/04/98 <90 6 12/04/98 <91 6 12/04/98 BACK TO MAIN li- * :C . f; .* :.0 I . 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J# C om m ents B A C K TO M AIN DILUTIONS SUMMARY SHEET Study: Dilution Date/Analyst: U Box#: p -O LY A il t MW Solvent/TN Number: , 'TArA~Z`i3$ Extraction Date/Analyst: flfyrfm Matrix/Tlmepoint: fle tte r sam p le n u m b e r . D ILU TIO N S or drscrpilion 1:fV 0 l:2 J v 1:25* 1: 1: 1: I: I: 1: Comments L t d . G5 '* UM . a* . aA 7 oA lt d 13d 73d 7y A lid V,/ X > i t i .A 77d lid nd. i7 d nA / C ,i * ( X . * * 3D A 3 \A * 33 d / V/ Notes:^,/ I 'SixO = 2.q u k^/o/c : et f l ^ L AUJitf / . A r t V.Oj^ a ^ tfc : 796/^l /vLoOfi A-2 5" ; 9 o / +.l M c O H . BACK TO MAIN DILUTIONS SUMMARY SHEET Study: 70K-/2 O Dilution Date/Analyst: S 1191,fA r Box#: 99'O CYjhA ' Solvent/TN Number: M dt, fc 'A -3H T ,,A Extraction Date/Analyst: pr ^ , fM JcP, r/A ^ Matrix/Timepoint:fojrU-xT .saomrpldeesncrupfmtbioenr 1:25 1:/0 1: I: 1: D1I:LUTIONS1: 1: 1: Comments X uW r 26/___ / 33/1 Al 3M 35/ 3(.rf 37/ ..3r^. 37/ VO/ i 37/ 2 37/ 30^ 3// ^ 42/ 63 43/ ^5/ 46/ ____________________ J J/ / x `/% \/ * Notes: A ( : 2 f ' H O ^ L /: i d - Sc.~fk M^OK .* 9 0 Q / - 1 - / u r i ! . .. ' BACK TO MAIN DILUTIONS SUMMARY SHEET J Study: -f'K-tQ.O _ Dilution Date/Analyst: ^ \ ! "t W r Box#: Jf-OCtiAyQ Solvent/TN Number: /M U, -taj- A - W t Extraction Date/Analyst: fiAfir /V?. f/tf Jc/ rpp a. ,, Matrix/Timepoint: P a t t e r * sample number DILUTIONS or deserpilion 1UD I: 1: I: 1: 1: 1: 1: . 1: Comments ' LI7M 6 3 \ * * * , ! / * . *. Notes: # ,( d o s OO^L To-yl^ ( ' 7 o/*1 4 * ' .* * * . # Study:'tox- o o DOntion Date/Analyst: s-s>n-97 f Box#; W - o io & A f b sample number or desrrpition V//*1 J 1: / 0 b io 1: BACK TO MAIN DILUTIONS SUMMARY SHEET , Sofrent/TNNumber: nuoti(tm-*-pjtoc, Extraction Date/Analyst: V '/f- mw- j q - j */*- j ,, Matrb/nniepoint! doj DILUTIONS 1: 1: 1: 1: 1: 1: Comments S-91-<?? S**-' . . ,, / * 0 *V Notes: (M O to 1 Qjf SOutnfil* q o o ^ L l?U.0tl y * J' . * ) BACK TO MAIN DILUTIONS SUMMARY SHEET BACK TO MAIN DILUTIONS SUMMARY SHEET Study: TbY'/^O Dilution Date/Analyst: Bx#; g oy 99-W A * & //J J _________________ sam p le n u m b e r . o r descrpition / o 1: 1: 1: Mm SOM w 0 /6 6H 1 m &H SM 6V sm oh 9 iM M SO I 6 4 (rH V L ___ Solvent/TN Number: M t fl / T*!~A ^ 3 2 / Extraction Date/Analyst: tJI f f a l J yCAiiJfccP/Sf/SAtJ _________ M atrix/Tim epoint: f r / fte r / f r U f Y ______________ DILUTION S 1: 1: 1: 1: 1: Comments * / N o tes: QI0-/OOmC SW pb t 9mL frM to o ! - ,* ' . - * BACK TO MAIN Sample weigJbits/Volumes Prep Date(s): Analyses): fttfkJ- JCi9"~ Sample Matrix: / ; r Method/Revision: / ' ''l * -f, O Target Analyte(s): / Study Number / 2-0 Equipment Number _ Notebook# and Page: Final Solvent/TN Number Sample ID Initial Wt/VoL g/mL/L ftnlM Q Water Added Density(g/ml) Comments 1MG1 2MG1 3MG1 4M Gl SMG1 ] 6MG1 . ' 6'flTT 3 3 /.9,/* f f ? 3 . 5 " ftr ff -2-- tCe> A.62X-L 3 M A.92UO H . S ,, 7MG1 8MG1 9MG1 A). 3 9/L5 /i.J T U .7 0.G 3W H fo q. ! 3.X '- 10MG1 # 9 7 2 3 .5 11MG1 V.3 ^ ---- 13MG1 ' Z . T / 5M 14M Gl 15MG1 17MG2 M /W 4 ftftr s // A573 V.!T V -3 r.3 > 18MG2 19MG2 20MG2 ' /7.W 93 /?,SX 39 5:0 p .? 3 .7 21M G2 22MG2 J 23MG2 24MG2 /. 3 fo 0 7avr o.STVo ' H.O *3 3C 3.0 '! *. 25MG2 ' 26MG2 27M G2 oummary oimcmoa; OfCrf 3>r n.-73fC 3 .7 0.9/7 * V.C ] fill p r s t i ed b y P *10 A lt -n * filer- Aexpo?, * CJ. ^ FAsT-n-J'O h /Z./ us* f eluder ( M J * y fJ j r r t*?er j // 9 U ytU ) e -X'*yt t* ) Notes: J & Ad/ rtf / /. r '' \ ]. Extraction-1 Template Review Date________ Form Approved by/Date:. \ Weight Volume Sheetl20.xls Page 1 of Prep Date(s): : Analyst(s): AtJhjep'J'AP Sample Matrix: / f j ^ Method/Revision: FAfT--fl-ftQ Target Analyte(s): . Sample ID 29MG2 30MG2 31MG2 33MG3 34MG3 35M G3 36MG3 37MG3 38MG3 39MG3 40MG3 41M G3 42M G3 . 43M G3 45MG3 46MG3 47MG3 49MG4 50MG4 51MG4 52MG4 53MG4 54MG4 ' 55MG4 56MG4 Summary of method: ] . Sample weightsolumes Study Number: J~0)Cf p Equipment Number Notebook# and Page: * Final Solvent/TN Number BACK TO MAIN Initial Wt/VoL g/mL/L #mlMQ Water Added Density(g/mi) /.ffSS' s w lu w r 327 3.H t.E m > St^HS.O t.tu ia s */./* 3 .0 a .si-w 3 . /. /tt 0.73C/S /.>U7- 5 '. V 3.-9 /.S' S, 3 w HA 3. i 0.T-/73 3 .L rt-iV/S- 3 , 2 .SS77, 2 . . S ZA .o ) ) : Comments .Y S-/9 S ..3 I i Notes: D ee xze Fonn Approved by/Date:. Extraction-1 Template' Review Date____________ _ Weight Volume Sheetl20.xls Page 1 of BACK TO MAIN ; . Sample weights/Volumes PrepDate(s): j A n a l y s t ( s ) : / i ^ ^ - J r / 7-' jfi- P ' Sample Matrix: /?t l-l-t*'/ Method/Revision: f^A-rTz-/7~/fO j Target Analyte(s): . Sample ID 57M G4 58M G4 . 59M G4 61MG4 62MG4 63MG4 65MG5 66MG5 67MG5 68MG5 69MG3 70MG5 71MG5 72MG5 73MG5 74MG5 75MG5 77MG5 78MG5 79MG5 81MG6 82MG6 83MG6 84MG6 85MG6 Summary of method: ] Study Number: *7" & ' Equipment Number: Notebook# and Page: * . FinalSolvent/TNNumber: - Initial Wt/VoL g/mL/L 0 .7 3? s.qnt 0.19S /. rrub> .onTX 0.90.3 la m o.70( D/9T73 O&Y 0.9377 o-tfiod 0.93/C t.ourc /. 007 / P'i%C 0.9900 0.993 0.73d? f.o9o3 om cb 0.9703 o.llQ f t.o n z #mlMQ Water Added H .r H.c ? .4 " ITV 3M 9.3 32. 9.0 (/.9 31 9.9 m. y 3.3 31 3.0 30 30 3 .* 3.3 97 .H.7 v.q 3 .9 *3 Density(g/ml) Comments J Notes: ! J Form Approved by/Date:. Extraction*1 Template Review Date___________ _ Weight Volume Sheetl20jds Page 1 of. BACK TO MAIN Sample weights/Volumes Prep Date(s): : Analyses): -) 1 Sample Matrix: fhf- Method/Revision: p fa 7-V /7 - / , p ] Target Anaiyte(s): . Sample ID 86MG6 87MG6 ] 88MG6 89MG6 90MG6 ] 91MG6 93MG6 1 94MG6 95MG6 ' 1 Study Number: "y~OTf~fXO Equipment Number: Notebook# and Page: . * . Final Solvent/TN Number Initial #mlMQ Water Density(g/ml) Wt/VoL Added (ghnL/L v:* &.Z37Z V r Z *,97Lt _ 4,4 l'ZCv S .Y t - o t t i s~,3 t'0T33 9oJZ Y .s: aAo IrOt-H jr ,l /,/ 0 J 7 S<S~ .. Comments J I i J J Summary of method: ] j Notes: % . j" I. J Extraction-1 Template Review Date___________ ! Form Approved by/Date:. . Weight Volume Sheetl20.xls Page 1 of, BACK TO MAIN Extraction Worksheet ETS-8-4.0 Study * - r a * f i,c Surrogate Std FC-Mix Box U A-i g approx ppm approx. 0.5 ppm Matrix a c t u a l p p m actual ppm 1___ n ? 9 > //^ 2 9__ -a XlW l- ___ ___________ _ ' --/U4L - SflA --/v 2-- -2*1__________ -i-k i. -.'r lA L f f l. ____ / a J ~Jaoapl-t ir FC-Mix approx. 5 ppm actual ppm # ? ? *//-/-! - FC-Mix approx. 50 ppm actual ppm - -. Date and Initials for Std. or Comments ------ 1 '! 1_ _A--------- 1---- 77------- M Cl____________ H M 6d___________ : m w m m am m SM C-1 LM ir ! V/ m & 9m 6-1 M 6-1 liM6-1 m 1 6! MMM ISM # / MM M mu -Ac------------ - ' 'IAJ. ID J fij- - - - - jj . VF -- DcP-sap-U JI i" * ' . - . - .- . - - - - . . . - :. I' - . _- .# 1 - #- . .- ... -Study num ber where the original worksheet is located. _Blank AU Serum Extraction Method Std * M o n - f - l r * amount itO P_________ * t t f r - M - t , _______ : ___mL A j / 7 i E . g / ) ' . Dat & Initials- Vortex 15 sec. V /rt/9 9 ______. Pioette M atrix. Pipette 1 ml of 0.5 M TBA, pH 10. Volume /, ml Std.# 99o,f-^2_ . -------------- ----------- v K l -- - . sm izu jU m m . Pipette 2 ml of 0.25 Na7COV0.25M NaHCOi buffer Std. # 99 0 / / - /S~A ' Dispense 5 ml o f methyl-t-butyl ether TN-A- XT* " Shake 20 min. Centrifuge 20-25 min. Centrifuge speed: 7T&& (Rem ove a 4 mL aliquot of organic laver Put on Nitrogen Evaporator to drvness Evaporator # : Temperature: Add methanol Volume 1.O ------- ml--------------- TZT3& & ------- / 1 Vortex 30 sec. 1 IFilter using a 3cc B-D syringe with a 0.2um filter into a 1.5 mi autosamole vial MS/MSD/ Cont. Checks: Spiked fo uL of a ppm std ( *7?>//-/.-! ) for a final concentration of /f .lf f i. ppm. MS/MSD used sample flj) . Cont. Checks used same matrix as for std curve. Surrogate Standard: Spiked jo uL of a S~ ppm std f 99o/}-/>-! )to all samples, standards, and blanks Q) f t J / h l U J r t 6 l } A ttach m en t'A ETS-8-4.0 Extraction o f PFOS from Serum Page 10 o f 13 BACK TO MAIN Extraction Worksheet ETS-8-4.0 Study #TG)f-130 SurrogateStd FC-Mix Box #9 approx. ppm approx.0.5ppm Matrix Liter actualJOO ppm actual ppm Woia-3^ # 1 1 M t o -_______ S"j i L - to - ____ 1_______ .- m to - FC-Mix approx.5 ppm actual ppm # - FC-Mix approx.50 ppm actual ppm # - - Date and Initialsfor Std.or Comments 1 -----1 a m to- &2M to - M M to- atM to - M M to - a m to- M M to HflAf t o to - . - aiM to VIM to - '- $A\ to % M to - r (J 3*M to M M 64 - - ViM 64 m 1 to 4tf/Vl t o H i M 64 VSVfl to % M to ?M U MM p/ U A to/ > L______: - - ' - >. _!^tudvnumberwheretheoriginalworksheel:islocated._____ ____________ amount= A C1 mL^inOife, M l Serum ExtractionMethod _____ '/r r L Date& Initials 1 Vortex 15 sec. Pipette Matrix Volume ml \ls Pipette 1 ml o f 0.5 M TBA, pH 10. Std.# s w i w u y/jjS Pipette 2 ml of 0.25 Na?CQV0.2SM NaHCOt buffer Std. # Y9/7/Z-rTF Dispense 5 ml o f mcthyl-t-butyl ether TN-A- 2X5T9 Shake 20 min. Centrifiiee 20-25 min. Centrifuge speed: 3St)f) Sp/W Remove a 4 mL aliquot of organic layer Put on Nitrogen Evaporator to drvness Evaporator ft: Add methanol Volume LO ml ______ Temperature: TO-A- g e a r fe> Vortex 30 sec. Filter using a 3cc B-D syringe with a 0.2um filter into a 1.5 ml autosamole vial MS/MSD//f Cont.Checks:Spiked I jiLofa ppm stdiW a IH L- I Uor afinalconcentrationof ' ppm. MS/MSD usedsample^!'/k e J'j r u J` Cont.Checksusedsame matrixasf'orstd`curve. SurrogateStandard:Spiked iO uL ofa <T ppm stdf 9^6)IHb-l )toallsamples,standards,andblanks 4)MSi/MPi-spt'u 3 M 6 - 1 1m ) M m - i p u M M , / u t t m a f ' Z Attachm ent A ETS-8-4.0 Extraction o f PFOS from Serum Page 10 o f 13 BACK TO MAIN Extraction Worksheet ETS-8-4.0 study m r - 1 3 0 SurrogateStd FC-Mix FC-Mix FC-Mix I Dateand Box approx, pptn approx.0.5ppm approx.5 ppm .approx.50 ppm Initialsfor Matrixl&f-Uver actual/00 ppm actual ppm actual ppm actual ppm Std.or #990/ # # # Comments 1 S b M (t H SMI V- __ S a i L ____ - - -. rsP-sty-uicAs f- I SVM Mi; C T M V- wrPr- 1___ --- &/H v1 StM 6-*f- J MM M M M (r*f feOVf C lM & C 1 M M Mr MM ^ M M T 1 WlvM 6S~ jm Mr J JMI (r<T VfM AT JtM M r 17M! tf-C TfM MT MAIMT Y M 6-to t i M irh > L______ - - -. - . - ** '- -. - - - - - '- -' - - __ it 1Studynumberwherethe original worksheet is located. J min* unr I Serum Extraction Method std # V i o l e t t --------______: amount = A U -- O______ II 1 III II 1 HI Date & Initials Vortex 15 sec. Pipette Matrix - Volume >\ 0 ml L' 1 I 1 5 3 Pipette 1 ml of 0.5 M TBA, pH 10. _____ Std. # GQa/f Pipette 2 ml of 0.25 Na?COV0.25M NaHCOi buffer S t d . # ^ / tS'O 1Dispense 5 ml of methyl-t-butyl ether ______________ TO-A- Z3-9 ] Shake 20 min. . 1 / 1Centrifuge 20-25 min. Centrifuge speed: Sp/Y\ -J ] Remove a 4mL aliauot of organic laver LPut on Nitrogen Evaporator to drvness Evaporator #: Temperature: / Add methanol Volume l- 0 ml TN-A- a W ~ I Vortex 30 sec. Filter using a 3cc B-D syringe with a 0.2um filter into a 1.5 ml autosamole vial 1 "37 J 0 . 3 H ) _ ppm. MS/MSD used sample ) InfJ/ru) . Cont. Checks used same matrix as for std curve. Surrogate Standard: Spiked 0 uL o f a ppm std i ff i b ih / l o - / )to all samples, standards, and blanks <D M S i / m p j - S f i l O i Z M 6rlj A fisa/M S& t-5f)ceU M lf M 3 M S P 3 ' S f i k z B / f t ( r i i Attachment A ETS-8-4.0 P age 10 o f 13 Extraction o f PFOS from Serum Extraction Worksheet ETS-8-4.0 BACK TO MAIN Study #_7DV-7? Box n w - ,fA iti Matrix E A f LlVPf Surrogate Std approx, ppm actual/00 ppm #9 0 V fT W . 'B m 1 _____s : MM ts-l* frU- . FC-Mix approx. 0.5 ppm actual ppm # - FC-Mix FC-Mix approx. 5 ppm approx. 50 ppm actual ppm actual ppm # - '- -- Date and Initials for Std. or Comments JW - s M J * \ IM I i l - M l e*U M &r- m . . M -__________ UM VM m , - 3 9*/M i. KM rt* 3/4t-M _ ,C /_ r-Tjr/> 1 --r i r M 6-! L________ - - fo J- - - - ' _ ' - . r-- /JM A-t - r r r { 4 MA-t - H rr> K _ - __ J T -- - 9- " ( im s j m p tf & ^ &<* OVUsi 1. A'TTtruJfLf I'AU Yv - oU-ipJiJ t\i Mas /o C O t s i h w //!$& ai r t i / 7. SA ti_v/l V f ? - u )h w 7(0/1M 'P tQ fh 27 _- _________ - -- -- * . v-v........ ___ _______ - '"All ^ IL --^ * 1Study number where the original worksheet is located. J Blank JSflfcfcrf LYtf Serum Extraction Method Std# amount' / i) I A cT-M~/. D mL Date &Initiab Vortex 15 sec. Pipette Matrix Pipette 1 ml of 0.5 M TBA, pH 10. Volume Std.# T ml </~2- ~]ZL Pipette 2 ml o f 0.25Na?CO^/Q.25M NaHCOt buffer Std. # ? g a / / -- ] CD Dispense 5 ml ofmcthyl-t-butyl edier Shake 20 min. Centrifuge 20-25 min. TN-A- I S T Centrifuge speed: 35VO/prt Remove a 4 mL aliquot of organic laver Put on Nitrogen Evaporator to drvness Evaporator#: Add methanol Volume A 0 ml Temperature: TN-A- S E S i Vortex 30 sec. Filter using a 3cc B-D syringe with a0.2um filter into a 1.5 ml autosample vial NL/ ~ 0`MS/MSD/ 8 C ont Checks: Spiked )0 uL o f a S~ ppm std ( ^ 6 ) / - } U*7 ) for a final concentration o f (7 ) ppm. MS/MSD used sampled?) t,fjA IaJ . Cont. Checks used same matrix as for std curve. Surrogate Standard: Spiked 10 uL of a A ppm std ( to all samples, standards, and blanks & M S J . m o h s p > & b M MS3//WP3 - y h flM l Attachment A --,JETS-8-4.0 Extraction o f PFOS from Serum Page 10 o f 13 . Ion Pair Standard Curves-TOX-120 (Rat Liver) BACK TO MAIN ~~ Prep Date(s): Analyses): Sample Matrix: Method/Revision: Target Analyte(s): 4/19/99 SAH/RWW/JCP/SRP Rat Liver FACT-M-1.0 . POAA, PFOS. , PFHS, M513 and M613 FC Mix Std Approx. 0.500 ppm: FC Mix Std Approx. 5.00 ppm: FC Mix Std Approx. 50.0 ppm: Surrogate Std Approx. 100 ppm: 99011-16-2 99011-16-1 99011-124 99012-3-24 ! Actual Concentrations ofStandards in the FC Mix POAA PFOS Std Cone. ug/mL Std Cone. ug/mL Std Cone. ug/mL j 0.500 j 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 I 0.500 j 5.00 5.00 5.00 r . 50.0 0.500 0.500 5.00 5.00 5.00 50.0 0.500 0.500 5.00 5.00 5.00 50.0 PFHS Std Cone. ug/mL 0.500 0.500 0.500 0.500 0.500 5.00 5.00 5.00 50.0 1 Calculated Concentrations ofStandards in the Sample Matrix. POAA Final Cone. PFOS Final Cone. Final Cone. PFHS Final Cone. ng/g ng/g ng/g ng/g 5.56 5.56 5.56 5.56 1 11.1 11.1 11.1 11.1 27.8 27.8 27.8 27.8 i 55.6 . 55.6 55.6 55.6 I ll 111 111 111 ! 278 278 i 278 278 556 i 833 556 556 556 833 833 833 1111 J 1111 1111 1111 Study Number: TOX-120 Equipment Numben Final Solvent & TN Number: MeOH/ TN-A-2438 Blank TIssue/IdentifieR Rabbit Liver / 99012-5-20 M513 Std Cone. ug/mL 0.500 0.500 0.500 0.500 0.500 5.00 5.00 5.00 50.0 M613 Std Cone. ug/mL 0.500 0.500 0.500 0.500 0.500 5.00 5.00 5.00 50.0 ' ah Ain't Spiked mL 0.002 0.004 0.010 0.020 0.040 0.010 0.020 0.03 0.004 AU Density g 0.180 0.180 0.180 0.180 0.180 0.180 0.180 0.180 0.180 M513 Final Cone. ng/g 5.56 11.1 27.8 55.6 111 278 556 833 1111 M613 Final Cone. ng/g 5.56 11.1 27.8 55.6 111 278 556 833 1111 Surrogate Std Cone. ug/mL 100 Surrogate Final Cone. ng/g 2778 AU Amt Spiked mL 0.005 Surrogate . Final Cone. ng/mL 500 1 ] I i POAAJFOS, .PFHS.M513.M613.xl5 BACK TO MAIN Ion Pair Extraction Worksheet Study # Surrogate Std FC M ix Std FC M ix Std FC M ix Std D ate and t *4 ' approx. actual m ppm ppm approx. 0.5 ppm actual ppm # w 9?o/t-/o-x approx. 5 ppm actual ppm #W tfo/Ui-l approx. 50 ppm actual ppm # w 99o?/*x*t Initials for Std. or Com m ents H ,0 B lank -[4 1ilAi,4- L>*er B lan k -/?, S~,J " zu tt ti- Qrf/ A l 1' / --f,,A- _ -- -- 1-------------- t t r f-- ---------------3 s ! 5 ? r __________ V J , ... [o j, 2oJ. ... -* -Li-In?'.A- V*J oJ . Soo'.A - ' StA- ] ___ J / -L l-, ~2sb.A-Ofti, nr ___ ptJ 3e>J j----------- (OJ Y ' 4/,)"aA* lo/.fa.---/i-l-2TWt. .M--S X ~}n.A Hfi) J*JL________ - f)MO*.1IDP~-(5`)~'2SJ, MS X -^ > ,.1 tlSD ---------1----------- t o d ,_______ iZ j -- t~ tfeiut-f f)\ oeS" /-*r / / / ra n > 1------- . .1 /r M r . ^ \cf) f F r 4----------- 1 _ -- -- __ .-- -- " _ _ -- -- ^ ^ ,-- " ---- ------------ -- --------------------- J ` -- :-- :-- Blank BAiitc,uer _-- - -- ------------------------ Std# ?X0?9-/S- 3 amount - /.Oa.t rt./c?* i Extraction Method Number/Rcvision: ____________ ___ < Date &In itials^ Add Surrogate. Vortex 15 sec. --------- --------------------- .----- ------------fnr4 WrJFSHSW Pipette Sample Volume ft V m L --------------- ,----------- ------------ . ........ - r ..., Pipette l mL of 0.5 M TBA. pH 10. Std. # 7*09< f A ------------- ------------------------- ] Pipette 2mLof 0.25 NapCCh/0.25M NaHCOi buffer Std. # <79OH-*? >>_ Pipette 5 mL ofrcthyf3eetatc AmJ-A/xtJfi TN-A- -2A===- ...................................... ------------- -- Shake 20 min. Centrifuge 20-25 min. _____ * ------------- ________ r.entrifiipc speed: ------------- ------------ ------- ... -- Remove a 4 mL aliquot of organic layer Put on Nitrogen Evaporator to drvness Evaporator#: ---------------------------- ----------- ------------------ Temperature: A* **/. . - Vortex 30 sec. ________ -- -- Filter using a 3cc B-D svringe with a 0.2um SRI filter into a 1.5 mL autosample vial . . . _ . Aro Additional Comments: -- ~r~ A----------- ^ --------------- oy [ _____________________________________ J . Ion pir Standard Curves-TOX-120 (RatLiver) BACK TO MAIN Prep Date(s): 2/22/99 Analyses): RWW/SAH Sample Matrix: Rat Liver Method/Revision: FACT-M-1.0 Target Analyte(s): POAA, PFOS, PFHS.M513&M613 Study Number: TOX-120 Equipment Number: Final Solvent A TN Number MeOH/TN-A-2325 Blank Tlssue/Identiflen Rabbit L iver/98099-15-3 FC Mix Std Approx. 0.500 ppm: FC Mix Std Approx. 5.00 ppm: FC Mix Std Approx. 50.0 ppm: Surrogate Std Approx. 16.5 ppm: 99011-16-2 99011-16-1 99011-124 98094-12-24 . Actual Concentrations o f Standards in the FC Mix POAA PFOS PFHS M513 M613 AU All Std Cone. Std Cone. Std Cone. Std Cone. Std Cone. Std Cone. Ain't Spiked Density 1 ug/mL ug/mL ug/mL ug/mL ug/mL ug/mL mL 8 0.500 0.500 0.500 . 0.500 ' 0.500 0.500 0.002 0.167 0.500 0.500 0.500 0.500 0.500 0.500 0.004 0.167 ] 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.010 0.020 0.167 0.167 . 0.500 0.500 0.500 0.500 0.500 0.500 0.040 0.167 5.00 5.00 5.00 5.00 5.00 5.00 0.010 0.167 5.00 5.00 5.00 5.00 5.00 5.00 0.020 0.167 1 5.00 5.00 5.00 5.00 5.00 5.00 0.030 0.167 SO.O 50.0 50.0 50.0 50.0 50.0 0.004 0.167 1 ' Calculated Concentrations of Standards in the Sample Matrix POAA PFOS PFHS M5X3 M613 Surrogate Final Cone. Final.Cone Final Cone. Final Cone. Final Cone. Final Cone. Std Cone ng/g ng/g ng/g ng/g ng/g m ug/mL 5.99 5.99 5.99 5.99 5.99 5.99 16.5 12.0 12.0 12.0 12.0 12.0 12.0 Surrogate 29.9 29.9 29.9 29.9 29.9 29.9 Final Cone. 59.9 59.9 59.9 59.9 59.9 59.9 ng/g 120 120 120 120 120 120 494 299 299 299 299 299 299 599 599 599 599 599 599 898 898 898 898 898 898 1198 1198 1198 1198 1198 1198 J ! M S/M SD 106F-G1 110F-G1 POAA Final Cone ____ ng/g J 106F-G1 110F-G1 ! 299 299 Initial W t feV 0.9240 0.9242 PFOS Final Cone . . ng/g 299 299 Initial V o lu m e tmt.V 4.6 . 4.6 Final Cone ng/g 299 299 PFHS Final Cone ng/g 299 299 MS13 Final Cone 299 299 M613x Final Cone ng/g 299 299 Amt Spiked fmLV 0.010 0.010 A ll Amt Spiked mL 0.005 POAA.PFOS, .PFHS.M513.M613 Sample Weights/ Volumes PrepDate(s): 2 ///if 1Analyses): fau SampleMatrix: fl.}Lu?/ Method/Revislon: /Vi<7'-/V7- I'V TargetAnalytefs): StudyNumber: EquipmentNumber: Notebook# and Page: FinalSolvent: /7 /XO . r<X Sample ID Initial (@rvoL /SmIVL. __ i*\lljrr 0 , m l S.t, f)fJ G9 ] V </ ______________ , J C c . /.? 3. _ i)ht* loi ___________ ^ _ 3.2 /) H a . QjJm M / /___________ us? 49* ! 2.2 SX DH?) ./OV/Z-Sl OtFV 41 ] ist. ! fido, t _____________ _ 0 , 9 4 L A. ?s 4U D H o . to7 fc-6-t OHo- (olP--l 3.7 >>4/20 S.l DaM. i i f -fi h.ixia 4.0 OaJo .DIF- FI 0 . 9 4 3 4 4.3- flAf. II'ZF~/y *1 b. 3.<ro\* . ntia. H4P-Cr2 h . W 9 r 3.2 OAli. t/ F " fr2 b.Wrf 3.4 r. U(o F'Crl h. 7 - ^ ? r 3.0 DrJ/, 17 F~6-2 J Pfln. // F~6~2 Daa .9P - 6 - 2 flAfri. /JO F-/-J /V/j. M P - F 2 3 1Ja m . / 3 3 . 6 C \ ? V 0.4U2 ' \.sr?91 f), 4 2 9 r 6.024/ 3.3 2.1 3.4 2.1 4.2 3.1 Comments gmlrtiL O./bl .IL& n./is O 'I 0 0 -----n.iOO .t(o4 f).(00 ./0S1______ 0: 6.11*3 ' 0./.T n.ibb QJJt - ______ 6.11*3 "O.OO (\U*7 6.1 or ojbr 6.100 C U 70 6,100 .M . Summary ofMethod: ] BACK TO MAIN j Notes: J Extraction-1 Template Review Date________ Form Approved by/Date:. Weight Volume Sheetxls Page 1 of Sample W eights/ Volumes BACK TO MAIN ' Prep Date(s): *z/ 1% Analyst^): fcUJU] /SAM 'Sample Matrix:jtf- LiFtf Method/Revision: p/fjZ./V[ ~J,Q Target nalyte(s): Study Number: '()(--/ P Equipment Number: Notebooks and Page: Final S o l r e n t : ^ / / / ; '. uJaUtr Sample ID Initial $pVoL yni IMr /0/lk I M F - & 3 ClAIa IZrF-irX " D aJ/). Id (jp~ DAln. 13 F F - P I OaIa .13 9 P - 6-3 ' A)a .136 F-P i PNn. M F - 6-3 ' O.MO o.m .mx b.Ufo _ _ /3.3/3.. fl.iaS -.0._ ?>fl. 3 . / .. AT. . 3.F- IOa Ia . JLfl..,, l O A W Z I P -6-1 .32H .. '4 A//J. I 4 P - 6 J O . M 3 I .. / ... . AIa . f F - i i (SSSM A 7 7 OaIa . lVoF-6-1 AlA.lteF-6* fi.SWI o . m H _ ^. j )AIa .)Z%F -fr3 &M3S-. 3-/ QAIa .itfF-firl ..C6-P0 f - A}n.M/P'/~l b.(o693 3.3 1 OAin.MXF-fr o,?*n .0 _ i A)n.Nlf-Pl 5*7 . 0 N //w S - 6 6.3(43 ht \ Ortf> * IW-6-4 ,w/!3 2.1 * Da/0. IWI/C-St J ' o.wr? 2.2 . Itl/fjC-A */ 0.4(99 2.5 1 F)40. ft/f-r 6-4 .of 7 to 1 tto. f S o f - 6 4 _____________ 0 , 6 4 1 6 lt2- Comments 3 /m l O.l(o? O.tlp* O.l(oF t u e , '____ o. Jio9 (MUT- O./faT Q.I06 M V k _____ o.i r .lU O.IUT-____ o.luZ g j u T ____ J5H n.n.' 9 O.Jfdfr O.Mob OMt 6.M fJUt .. O.M M O ? ____ , J, - J Summary of Method: I Notes: J . Extraction-1 Template Review Date____________ I ^ F o r m Approved by/Date:_______ ___________________ -- Weight Volume Sheetxls Pa8e * * Sample W eights/ Volumes BACK TO MAIN Prep Date(s): /if/?*) wdyrtsy. r pample Matrici /lif-Lt Method/Revidon: Target nalyte(s): 6 Study Number: / OA-/ Equipment Numben Notebook# and Page: Final Solvent: fi,//, ?/ 'S Sample ID Initial @fVoL /#mI7L tw i M akr d tf t '/j O r f/r tL Comments D r/n ir ft -----_'_--_-_--_-_--_-_--_-_-- f i J f f , f 7 7 - p - -- S r n __________ 6 ' C 3 1 \ - ...Jn/ f^T/U) t.u tirr itr ik ? kLmmf........ - 1{n / (mT i L?J, f/ / y hr.-f f ) / r $ T ~ ~ --- * - / *______ /> .X S 7 f l , j> /9 11. I \ !Hj *e 1. ---- 6=--< ff--------------------- o . H f ! /)jJ a . tr U F - S r * / ,r y s i> h r t f f . /Ir? f t u a , t C / X - / S-5" t 1 X l9 /)// . //. 2 j - - " T ,/! /) h/ v/ A *. {/ U' i* */ J r --------------------- /5r * 7 * 7 j!A a J . U /'*... - A-WO/ir..... --........-- > 7 7 9 /)h /r> . 0 `S " * ,7 7 /9 f t t a , //. X - A - X ,</JLT7 Dhf. f 7 / ? - C r X , 3 7 ? r r f / ) f ) t / a , n s ) X -- a -x ff /)j fN//n/ , ( 7 / I/ r..... >r ,c............ T )N . tn 3 X -~ /z X /- / ) r J A , 7//- ~ / C r ,T J < H /9 , 7 7 X 7 . T C 7 ! t T7A, 0 .1 1 3 7 H a, 3 D tfo , n x - /1 < T A -r . A . < / ,! A. 7 7 J~2. * firto . f 7 f ------------ a y ^ - ? 2 .7 3 .2 2 , 0 ... .. 7 - 7 __ 4C9 _ 3M _ J & /___ v X? 2 ./ 2V ? .2 ?- W 9 .0 ZI 7 3 2 0 .1 1 * 1 f h / > r ____ .lU b d l i l ____ J b (o a / 6 ? ____ 0 -/4 ? jM L D .M ./w ~ ' ... ____ o ./fci- (\.! W h ./W ( \ . I W ______ 0 fl(p (? D M ______ f ld b n .ll/G n . ,? n .iu r r t,/6 7- I Summary of Method: 'f . Notes: Extraction-1 Template Review Date.____________ Form Approved by/Date:.--------------------------------------- - Weight Volume Sheetxis Page lo i Sample Weights/Volumes Prep Date(s): i f i f fa ? Analyses): Atf Sample Matrix: A *//< <' sr Method/RevUlon: /Q f/ 7 '-/H7 - / , Target Xnalyte(s): Study Numben *J~0/ - / LQ Equipment Number: Notebook# and Page: . Final Solvent: fit ll, # V*. 7*f Sample ID Initial \hf*4*r k/VoL OfxnUL .... ft^ l $ m/ m C pA/a. 7 9 ? P'SVSJ- . 7 ~ n 7 lW PNO. ( f t f - 4 Drifi. [ ZLf - 6 6 ,? r* t sf.D/e? ,r?YZ> JL_____ J,L 3,0 o .iu r ry.r&r o.i(,sr nw. 'nnf P r f r / i . Y<3, o./or 'ppn. f ? f f - -r itrf-/it f)r/ f ( Tr t"~--/7 /s .... y . t ... /.0T7 s:3 ,? S < rr 0 .1 1,7 O.Kefo O.lb (AfP. /r(. A ln Z J,Z O.lbtfi AfP. W f - Sri 0 ,i7 i^ ?'</ o.iuo- A/0. ( f O p - f r C trCTf? J ^ f 0 .10 2 Philft- / T _ /r C ` 6,9r id Y-'L OJU, Comments BACK TO MAIN J Summary of Method: ] rr I! Extraction-1 Template Review Date____________ Form Approved by/Date:___________________________ ' i_ Weight Volume Sheetxls Page 1 of BACK TO MAIN SOUP 020199: FACT Equipment Log 99014 Quattro II, #6414E PAGE DATE: 2/23/99 ANALYST: drb STUDY NUMBER:Tox-120 MATRIX: Rat Liver DESCRIPTION: Liver Sam ples. FACT Method: FACT-M-4.0 . Injection volume: 10 uL Cycle time: 13.5 min. Electrode: Z-Sprav MobDe Phase: fA12.0 mM NH4CH3CQ2 OBIM cO H TN-A- or stndrd number (A) 98012-1-4_________ fBl TN-A-2260 Prepared (date/by): Expires: (A1 2/11/99 hoi________ (A) 8/99 ____________ (B1 2799. hoi (B) 8/99 Store at room temp? @ or N Other:___ G u ard colunm: None__________________ Part number: na_________ Lot number: na A nalytical column: Keystone Betasil____________________________________ Size: 2 x 100 mm________ - : ' Type: C l 8____________ Sphere size: 5 um_______ Part num ber 105-701-2__________ Lot number: P8C09 Serial number: 10881106D Analytical param eters: Drying gas (L/hrl: 300 . ESI nebulizing gas (L/hrl 15 LC Flow rate: 300 uT/tnin. Back pressure: 120 bar Sheath gas (L/hrl 0 .S ' HPLC solvent gradient: Long 2 Time (min.l 110-00_____________ 2 ) j i5 Q __________ 3111.0 _______ 4112.0 51 13.5_____________ %B 40.0 mo. 90.0 40.0 Stop - Tuning Method Report Method: C :\MASSLYNX\FACT-1.PRO\ACQUDB\PFOS Printed: Tue Feb 23 15:59:26 1999 _ ____________________________________________;___________ Jouj) c a o m Ot IAC o i l olii BACK TO MAIN Page 1 , SOURCE ( ESP- ) 1] Capillary Cone Extractor I RF Lens i Source Block Temp. Desolvation Temp. 1 | Pressures } Analyser Vacuum Gas Cell J1 . ! J .i! J Set Rdbk 3.00 60 5 0.2 150 250 2.99 -60 -6 142 250 Rdbk 7 . 8e-6 FAIL MSI LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier Set Rdbk . 14.0 14.0 *' 2.0 0.0 55 650 MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier Set Rdbk 15.0 15.0 45 2.0 0.0 40 0 650 42 39 44 I ] 1 ] 1 1 Printed! Tu Feb 33 1 8 : O O U 3 199 Suj) 20M __ Solvent Dly ( mins ) i 0 .0 0 Function t 1 KRH of 3 HaS a f a i n ( U P - J Inter Channel Delay ( Secs ) i Span ( Daltons ) i Start T i m ( Mins ) t End T i m ( Mins ) t Repeats t Channel Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell (Secs) Coll Energy (eV) Cone ( V ) 1 399.00 80.00 0.25 2 399.00 99.00 0.25 3 399.00 130.00 0.25 45 45 45 50 50 60 (AC os/loll Function t 2 M W of 3 Mass Fairs ( U P - > Inter Channel Delay ( Secs ) t Span ( Daltons ) t Start T i m ( Kins ) t End T i m ( Mins ) i Repeats * Channel. Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell . (Secs) Coll Energy (eV) Cone 1 499.00 80.00 0.25 2 499.00 99.00 0.25 3 499.00 130.00 0.25 45 45 45 60 60 60 Function i 3 XXM of 2 Maas Fairs ( ESP- ) Inter Channel Delay ( Secs ) i Span ( Daltons } i Start T i m ( Mins ) s End T i m ( Mins ) ; Repeats $ Channel Parent Daughter 0.03 0.00 0.00 13.50 1. Dwell (Secs) Coll Energy (eV) Cone 1 299.00 80.00 ' 0.25 2 299.00 99.00 0.25 45 45 35 35 V) Function i 4 MRX of 3 Mass Fairs ( ESP- } 1 Inter Channel Delay ( Secs ) i 0.03 I Span ( Daltons ) t Start T i m ( Kins ) < * 0.00 0.00 End T i m ( Mins ) I Repeats t 13.50 1 Channel Parent Daughter Dwell (Secs) Coll Energy (eV) Cone ( V ) ]1 2 413.00 119.00 413.00 169.00 0.25 0.25 3 413.00 2 19r00 0.25 30 20 30 15 15 15 Function i 5 KRK Of 3 Maas Palra ( n r - ) J Inter Channel Delay ( Seca ) t 0.03 Span ( Daltons } t 0.00 Start T i m ( Mins ) t 0.00 End T i m ( Kins ) t 13.50 Repeats i 1 Channel Parent Daughter Dwell (Sk i I Coll Energy (eV) Cone 11 469.00 169.00 0.25 45 . 30 '2 469.00 219.00 0.25 45 ' 30 3 469.00 119.00 0.25 45 30 ] 1 1 : i rmetloB 1 m of 3 M u . > Int.r Channel Dl.y ( Secs ) t Span { Dalton. ) i Stare Tin. ( Kin. ) i Bnd Tia m ( Kin. ) i Repeat. i > Channel Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell (Seca) Coll Energy (eV) Cone 1 569.00 169.00 0.25 3 569.00 219.00 0.25 3 . 569.00 119.00 0.25 45 45 45 35 35 35 Function i 7 m m of 1 M u a Fair ( w - ) Intar Channel Dalay ( Saoa ) i S p u ( Dalton. ) > Stare Tina ( Min. ) i *nd Tim. ( Mina )' i Sapaata t Channel Parant Daughter 0.03 0.00 0 .Q0 13.50 1 Dwell (Seca) Coll Energy (eV) Cota ( V 1 627.00 90.00 0.25 40 60 BACK TO MAIN BACK TO MAIN M asslynx - Sam ple List Sample List: C:\MASSLYNX\FACT-1.PRO\SampleDB\022399c.SPL Printed: Tue Feb 23 16:25:201999 ~ in a _____________________________________ S n u p 0 2 0 L M ,i LAC, o s / b i n Page 1 File Nam e File Text . MS R ie Inlet 1 Botti olum e Sam ple T] C o n c A C o n c B C o n c C 1 022399020 MeOH Blank TOX12C 2 022399021 MeOH Blank TOX12C 3 022399022 Tox 120, RBL02229-H2O Blk-1,2 /2 2 TOX12C 4 022399023 Tox 120, RBL02229-RBLiverBlk-1,2 , TOX12C 5 022399024 Tox 120, RBL02229-5ppb-1, 2/22/99 TOX12C 6 022399025 Tox 120, RBL02229-10ppb-1,2/22/9 TOX12C 7 022399026 Tox 120, RBL02229-25ppb-1,2/22/9 TO X2C 8 022399027 Tox 120, RBL02229-50ppb-1,2 /2 2 /9 TOX12C 9 022399028 Tox 120, RBLQ2229-100ppb-1, 2/22/ TOX12C 10 022399029 Tox 120, RBL02229-250ppb-1,2/22/ TOX12C 11 022399030 Tox 120, RBL02229-500ppb-1,2/22/ TOX12C 12 022399031 Tox 120, RBL02229-750ppb-1,2 /2 2 / TOX12C 13 022399032 Tox 120, RBL02229-1000ppb-1,2/22 TOX12C 14 022399033 MeOH Blank TOX12C 15 022399034 Tox 120 DNO. 97F-G1, 2/22/99 TOX12C 16 022399035 Tox 120 DNO. 98F-G1, 2/22/99 TOX12C 17 022399036 Tox 120 DNO. 99F-G1, 2/22/99 TOX12C 18 022399037 Tox 120 DNO. 100F-G 1,2/22/99 TOX12C 19 022399Q38 Tox 120 DNO. 101F-G1,2 /2 2/9 9 TOX12C 20 022399039 Tox 120, RBL02229-250ppb-CC1,2 / TOX12C 21 022399040 MeOH Blank TOX12C 22 022399041 Tox 120 DNO. 102F-G1, 2/22/99 TOX12C 23 022399042 Tox 120 DNO. 103F-G 1,2/22/99 TOX12C 24 022399043 Tox 120 DNO. 104F-G 1,2/22/99 TOX12C 25 022399044 Tox 120 DNO. 105F-G1, 2/22/99 TOX12C 26 022399045 Tox 120 DNO. 106F-G 1,2/22/99 TOX12C 27 022399046 Tox 120, RBL02229-250ppb-CC2, 21 TOX12C 28 022399047 MeOH Blank TOX12C 29 022399048 Tox 120 DNO. 107F-G1, 2/22/99 TOX12C 30 022399049 Tox 120 DNO. 109F-G1, 2/22/99 TOX12C 31 022399050 Tox 120 DNO. 110F-G 1,2/22/99 TOX12C 32 022399051 Tox 120 DNO. 111F-G1,2 /2 2/9 9 TOX12C 33 022399052 Tox 120, RBL02229-250ppb-CC3, 21 TOX12C 34 022399053 MeOH Blank TOX12C 35 022399054 Tox 120 DNO. 113 F -G 2,2/22/99 36 022399055 Tox 120 DNO. 114F-G2,2/22/99 TOX12C TOX12C 37 022399056 Tox 120 DNO. 115F-G2, 2/22/99 TOX12C 38 022399057 Tox 120 DNO. 116F-G2, 2/22/99 TOX12C 39 022399058 Tox 120 DNO. 117F-G 2,2/22/99 TOX12C 40 022399059 Tox 120, RBL02229-250ppb-CC4, 21 TOX12C 41 022399060 MeOH Blank TOX12C 42 022399061 Tox 120 DNO. 118F-G 2,2/22/99 TOX12C 43 022399062 Tox 120 DNO. 119F-G 2,2/22/99 TOX12C 4 4 . 022399063 Tox 120 DNO. 120F-G 2,2/22/99 TOX12C 45 022399064 Tox 120 DNO. 121F-G 2,2/22/99 TOX12C 46 022399065 Tox 120 DNO. 122F-G 2,2/22/99 TOX12C 47 022399066 Tox 120, RBL02229-250ppb-CC5, 21 TOX12C 4 8 022399067 MeOH Blank TOX12C 49 022399068 Tox 120 DNO. 123F-G 2,2/22/99 TOX12C 50 022399069 Tox 120 DNO. 125F-G 2,2/22/99 TOX12C 51 022399070 Tox 120 DNO. 126F-G 2,2/22/99 TOX12C 52 022399071 Tox 120 DNO. 127F-G 2,2/22/99 TOX12C 53 022399072 Tox 120 DNO. 129F-G 3,2/22/99 TOX12C 54 022399073 Tox 120, RBL02229-250ppb-CC6, 21 TOX12C 55 022399074 MeOH Blank TOX12C 56 022399075 Tox 120 DNO. 130F-G 3,2/22/99 TOX12C 57 022399076 Tox 120 DNO. 131F-G 3,2/22/99 TOX12C 58 022399077 Tox 120 DNO. 132F-G 3,2/22/99 TOX12C 59 022399078 Tox 120 DNO. 133F-G 3,2/22/99 TOX12C long2 long2 long2 long2 !ong2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 1ong2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 91 91 1 2 3 4 5 6 7 8` 9 10 11 92 12 13 14 15 16 17 92 18 19 20 21 22 23 92 24 25 27 28 29 93 30 31 32 33 34 35 93 36 37 38 39 40 41 93 42 43 44 45 46 47 94 48 49 50 51 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Analyte 10 Analyte . . 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10" Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte ' 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte . 1 ' C M asslynx - Sam ple List Sam ple List: C:\MASSLYNX\FACT-1.PRO\Sam pleDB\022399c.SPL Printed: T u e Feb 23 16:25:20 1999 c 5ni^ lP.lMa _____ti tt*C Q- .S. A/IqPIa La l , F ile Nam e File Text M S File Inlet I Botti olum e Sam ple T 60 022399079 Tox 120 DNO. 134F-G 3,2/22/99 TOX12C long2 52 61 022399080 Tox 120, RBL02229-250ppb-CC7, 2 / TOX12C long2 53 62 022399081 MeOH Blank TOX12C long2 94 63 022399082 Tox 120 DNO. 135F-G 3,2/22/99 TOX12C long2 54 64 022399083 Tox 120 DNO. 136F-G 3,2/22/99 TOX12C long2 55 65 022399084 T o x 120 DNO. 137F-G 3,2/22/99 TOX12C long2 56 66 022399085 Tox 120 DNO. 138F-G3, 2/22/99 TOX12C long2 57 67 022399086 Tox 120 DNO. 139F-G 3,2/22/99 TOX12C long2 58 68 022399087 Tox 120, RBL02229-250ppb-CC8, 2/ TOX12C long2 59 69 022399088 MeOH Blank ' TOX12C long2 95 7 0 022399089 Tox 120 DNO. 141F -G 3 ,2/22/99 . TOX12C long2 60 71 022399090 Tox 120 DNO. 1 42F -G 3,2/22/99 TOX12C long2 61 72 022399091 Tox 120 DNO. 1 43F -G 3,2/22/99 TOX12C long2 62 7 3 022399092 Tox 120, RBL02229-250ppb-CC9, 21 TOX12C long2 63 74 022399093 MeOH Blank TOX12C long2 95 75 022399094 Tox 1 2 0 ,1 06F-G1-250ppb-MS, 2/22/ TOX12C long2 64 76 022399095 Tox 1 2 0 ,1 06F-G 1-250ppb-M SD, 2/2 TOX12C long2 65 7 7 022399096 Tox 120, RBL02229-250ppb-CC10, TOX12C long2 66 78 022399097 MeOH Blank TOX12C long2 95 79 022399098 Tox 120, RBL02229-H2O B lk-2,2/22 TOX12C long2 67 80 022399099 Tox 120, RBL02229-RBUverBlk-1, a TOX12C long2 68 81 022399100 Tox 120, RBL02229-5ppb-2,2/22/99 TOX12C long2 69 82 022399101 Tox 120, RBL02229-1 Oppb-2, 2/22/9 TOX12C long2 70 83 022399102 Tox 120, RBL02229-25ppb-2,2/22/9 TOX12C long2 71 84 022399103 Tox 120, RBL02229-50ppb-2,2/22/9 TOX12C long2 72 85 022399104 Tox 120, RBL02229-100ppb-2,2 /22/ TOX12C long2 73 86 022399105 Tox 120, RBL02229-250ppb-2,2 /22/ TOX12C long2 74 87 022399106 Tox 120, RBL02229-500ppb-2, 21221 TOX12C long2 75 88 022399107 Tox 120, RBL02229-750ppb-2, 21221 TOX12C long2 76 89 022399108 Tox 120, RBL02229-1000ppb-2, 2/22 TOX12C long2 77 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard BACK TO MAIN Page 2 Cone C ( 1 Method Report Method filai U l C odi fiad i Cl \HASSLYNX\FACT-1 .9RO\HethI\022199c .edb Bai Feb 29 13125114 1999 ] C-pOUHdl Printed! 2t prat Med Her 03 lSt41i29 1999 C a p o u n d Me-- t VPMS Internal Ref.i (None) Quantify Tracei TIC 1 Cone, of Standards! Cone. A User RP Valuet User Peak Factori 11..000000000000. Dieabled Integrate Window Faeton 2.000 I Retention Ti-- (nine) t . Relative Retention Ti-- 1 4.5C3. enabled 0.000. Disabled Ti-- Window (nine) t 1.117 Peak Selection! Nearest 1 Reaponaa Typet Response 1 External . Areas ] ] I Polynomial Type* Point of teigini Fit Weighting! Axie Tranafoc-- tleni Units1 . Peak-to-peak amplitudet --00thing! Window else (aeons) 1 Kueber of s-- othsi t-- o thing -- thodi Linear Exclude NoNona 1*5 Disabled 1 2 * Join valley threshold]. Reduce peak tailing] Raise baseline -- x i e u n 30.00 70.00 4.00 Drew vertical threshold! Deteet shouldersi Shoulder threshold! 10.00 Disabled 10.00 Relative height! Absolute height! Relative or-- ! Absolute area] ' 50.00. b a b i e d 0.00, Disabled 3.00. b a b i e d 0.00, Disabled Soup p a o i q ^ BACK TO MAIN Pag 2 u c , 0 3 /0 3/ ^ ^ Mathod Report Hathod filai Last aodiflads Conpound Fr intads Ct \KASSLYNX\PACT-1.PR0\MatbDR\022399c.ndb Thu Fab 25 13:25tl4 1999 3t FORA Wad War 03 1Ci4 1 29 1999 Internal Raf.t Quantify Trae*i Cone, of Standardst Vaar w Valuai Usar P M k Faeton Integrata Window Faeton MU IWon*] TIC 11..000000000000. Diaablad 2 .0 0 0 Fataneion Tin* (niaa) i Salativ* Retention Tinas Tin* Miniar (mina) t Peak Salactioni Raspansa Typai Responses 7.450. b i a M a d 0.000. Diaablad 0.902 External Araas Folynoadal Typai Folnt of Origini Fit Weighting* Axis Tranafomation t ^ Uaitai F*ak-to-p*ak aaplitudai Sanothingi Window also (scana)t Nunbar of aeothsi soothing aathodi D1iaablad 2 Join vall*y tbrasholds Raduca paak tailings Raisa haslina aarinns 30.00 70.00 4.00 Draw vortical thraholdi Datact shoulders s Sbouldar thraaholdt 10.00 Diaablad 10.00 Ralativ* haighti Abaolut* heights Ralativ* araas Abaolut* araas 50.00. Cnablsd 0.00. Diaablad 3.00, Xaablod 0.00. Diaablad _____ Soup BACK TO MAIN -. ;; ___lAiL_ OilW i l Faga 3 Method Report Method C i U l U i t aedifiodi Cospoundt Printed* Ct\MASSLYMX\rACT-l.PRO\MethDS\022399e.mdb Thu Fob 25 13*25*14 1599 4 1 M513 Med Mor 03 14*41*29 1999 Ceeyound Nano* Internal Ref.* Quantify Trace* Cone, of Standards* User RP Value* Veer Peak Factori Integrate Window Factor* M513 (Mono) TIC Cone. A . 11..000000000000, Disabled 2.000 Retention Tino Inins) t Relative Retention Tine* Tine Window (nine) i teak Selection* S.44S. b a b i e d 0.000, Disabled 0.730 Hearest Response Type* Response* 3 Polynomial Type* Point of Origin* Pit Weighting* Axis Transformationi External Areas units* 1 Peak-to-peak amplitude* Smoothing * 1 Window site (scans)* Humber of snooths* Smoothing method* 2 Join valley threshold* Reduce peak tailing* Raise baseline maxim* Draw vertical threshold* Detect shoulders* Shoulder threshold* 30.00 70.00' 4.0Q 10.00 Disabled 10.00 Relative height* Absolute height* i Relative area* Absolute area* 50.00, b a bied 0.00, Disabled 3.00, b a b i e d . 0.00, Disabled BACK TO MAIN S o u p 2 6 M ______ H e Ol/ollw 9*00 4 '-**. > Hathod Rapert Method filai Last nodifiad Coapoundt Frintedi Ct\MAS5LYMt\FACT-l.PR0\KetkD\O22399e.db Thu Fab 23 13r25i14 1999 Si M<13 Wad Mar 03 Ki41 i 2 9 1999 Intanai Mf.i Quantify Tracci Cone. of Standard*! Usar RF Valuai Vaar Feak Faeton Integrata Wlndow Faeton M13 tuonai TIC 1.000000. Diaablad 1.000000 2 .0 0 0 Rataation Tina (adna) i Relativa Rataation Tinai Tina Wlndow (nina) t Faak Salaetioat 9.391, Knabled 0.000# Diaablod 0.730 1 Raapooaa Typai Extamal Ara** tolyaonial Typai Foint of Origini Fit Walghtingi Axia Trans f o n a tieni Linear Include 1 fask-to-peak anplitudat Swothingi Wlndow alca (aeana)t 1 ttanbar of anoothai fnnothlng nathodi Join valla/ threaholdi Reduce peak tallingt Raiaa baseline aaxinuai Draw vartieal thrasholdt ! Detect ahouldarai I Shouldar thrasholdt i ------------- Relativa haighti Absoluta haighti Relativa arasi Absoluta arasi Ditabiad 1 2 30.00 70.00 * 4.00 10.00 Diaablad 10.00 30.00, Enabled 0.00, Diaablad 3.00, Inablad 0.00, Disebi ad BACK TO MAIN - / So u p .,.D3o.tiq___ :___ (AL___ :0 3 /0 3 /9 '? Faga S Hatiio d Rap o rr Method filo* - C>\NAMLYKX\PACT-1.9ft0\MethDR\O22399e.wtb Last aedi fiad* Ttu Pb 25 13*25*14 1999 Ccepouadt 4t U . Printad* Wd Kar 03 14*41*29 1999 CompoundMmi In tern a i M ..I Q uan tify Trac* con e, o f Standard* Uaar RP Valu* Uar Paak Pactor* Intgrt Hindou Pactor* Is ne(No m ] Cne. B 1.000000, Disabled 1.000000 2.000 Rtention Tina (aina)* . Relative Rotention Tina* Tina Hindou (aina) * Paak Sletlent 7.410, Enabled 0.000, Disabled 0.94 Nearest Rasponaa Type* Raapons* External . Areas Pelynaalal Type* Paint ef Origint Fit Meightiag* Axis Transformation* Linear Exclude None No m . * unita* Feak-to-peak asplitude* its - Metbing* Hindou sire (scana)i Nuaber of maotha tanolrhing nathod* .. Disabled 1 2 Joln va11y threahold* Rduc paak taillagi Rai baseline maximum* ` 30.00 70.00 4.00 Draw vertical threahold* Datact ahouldarai Shoulder thresbold* 10.00 Disabled 10.00 Ralativ baight* Abaelut baight* Ralativ arat Abaelut ar*a* * 50.00, Inabled 0.00, Disabled 3.00, Enabled 0.00, Disabled . _______ Soup . . ' BACK TO MAIN hg c j} lAL Oli*/m ____ FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN Date: Analyst: zie; FACTStudyNumber / OY. /<0 Analyte 4: /yi S /3 Acceptable LinearRange: 573 - 7 5~o Matrix Time Points Dilutions? 3 Analyte 1: 1 AcceptableLinearRange: 5 - S & 0 IaT* h LOQforthis dataset -S' C C '5 r PASS ? Re-evaluatedfar different range? Flht r\ties ft cliiuW\ lAl LOQforthis dataset: CO\' " tsUtA k,rRe-evaluatedfordifferentrange? DiluW y p 3 ftfoaiJqy? ...... 0/* S b U d i \ ^ . AnalyteS: /r? 6 / 3 AcceptableLinearRange: ~5?>Q LOQforthis dataset: CC '>; f r z s ^ Re-evaluatedfordifferentrange? _______ Analyte 2: AcceptableLinearRange: 5~~ 5 & /^oL Analyte 6: -=zs 677*- OSfVsVM/c- / Y ^ o H i - A ^ j O / Z & i AcceptableLinearRange^ ' LOQforthis dataset a??5 / _____ CC !s r /yioi-r '' LOQforIbis dataset: Re-evaluatedfordifferentrange? J V t & f /fccZ*T7<)4C`-- l -- c ^ w e /vor / & 'ffYT'* Analyte3: PoAA Analyte 7: 3 AcceptableLinearRange: /O --SV&jQfb Acceptable LinearRange: LOQforthis dataset: CC%: ' Re-evaluateafor different range? LOQfards dataset: Re-evaluatedfordifferentrange? fctole.* R e a n a l g i ^ -G>r PF|)$ Q f i A d J u i l i O n i I`.S' G y 3 ftr M 5/3 MC s sre fsffre ffflff ffffff ffffff ff??? fff?ff ?f?ffff|ff|Irlff??*ml mI2 5 5 * 5 5 5 5 * * * * M > M * a s * H H H H h H H H H * * IslslsSSu. sssssssss >jsij goiOaei 5 s22ssss gg g .g g g ss S i S S tzal*JA aj^vl Ila I O 522 S S 522225 liii m i l i g S rjrr"" r 522255 gg g S -s S m mCB<aj<!>J*aj f " f " ?* ' 552252 IsSSSSKS,, SSSSSSSSS ekehk sa issssss-a^o SKSSSSSXSS KMOHWNOH ^militi oIMOiAIHlAVU L1d0lJ OP10WMIANUHI*I AMMaUlM0I4U0INMIJMNUMtWalMW<N0 I1BN0MONMAMMM04MA4014MM0A0UMi4 AMN M0ImN4MCAgBM01w40MuAtJbbMOl oSg4KtoHBgSMKgKg CAMA MIA*JNMAM10HA1M0HkMAlHOAM 0s1a0s0 0s*O*H.W. 401O*NOU OajOOAMO OO M01* M1 s u iiii MOJA0H10.MH0A4W(fl t*M04AIOMUOI AW M0M1AM MmOO!IANAJ oWH WutWuiUo Wg IN KMMl0O AU WA UA NO OMWOHlIHHH-WJHW H-tot-oa-aaigoin MW C0B4O*4M04M04ICBN Uglw40gA H p10wA Am aM gA aA mM pM M1 MMi MUf 1Mi0 1 MM iiiiii n i n i liiiii iiiiii iiiiii AAMM A*Ag4.MOMHMMOOAM.UMMAt AAAA *M(O00A0M100OMUH WMAM OAMM M UUMAMuOAOMMMCMAAUUMAfUlAA AgAJOwM10McAalAMA-AJuO*A0pABMAgCIAU0uHwUJ OpO1K(0lMM1S0AK1)04g0 M4M0AOM MAH MA AA AJ M104AAA0 AA AO AA 4OM0 aIH0OgAOaOAMAM4W0WAOMIMMO U COSAJHO CABACB O-4-AJMAWMO104C0B A 1A0-Aal4O0MA A10 WAAOUIi0gAiMOHAUMOOtAtAM AM MA1 MM1 I4IMMI0IMMMMOMAM lini l i m i i n n i miiij '! BACK TO MAIN Quantify Confound Sunnary Raport Tox 120 DNO, Jlat Livar Extracts Saaple H a t : C:\KASSLTOX\FACT-l.PBO\8a^pleDB\022399c Laat nodiflad: Thu Tab 23 13:24:27 1999 Method: C:\HAaSLXHX\FACT-l.PRO\HethDB\022399c. L u t nodi lad: Thu Fab 25 13:25:14 1999 job Coda: Printad: Thu Fab 25 13:43:49 1999 ' Confound 2: PFBfl Kama 1 022399020 2 022399021 3 022399022 4 022399023 5 022399024 6 022399025 7 022399026 8 022399027 9 022399028 10 022399029 11 022399030 12 022399031 13 022399032 14 022399033 15 022399034 16 022399035 17 022399036 18 022399037 19 022399038 20 022399039 21 022399040 22 022399041 23 022399042 24 022399043 25 022399044 26 022399045 27 022399046 28 022399047 29 022399048 30 022399049 31 022399050 32 022399051 33 022399052 34 022399053 35 022399054 36 022399055 37 022399056 38 022399057 39 022399058 40 022399059 41 022399060 42 022399061 43 022399062 44 022399063 45 022399064 46 022399065 47 022399066 48 022399067 49 022399068 50 022399069 51 022399070 52 022399071 53 022399072 54 022399073 55 022399074 56 022399075 57 022399076 58 022399077 59 022399078 60 022399079 61 022399080 62 022399081 63 022399082 64 022399083 65 022399084 66 022399085 67 022399086 68 022399087 69 022399066 70 022399089 .71 022399090 72 022399091 73 022399092 74 022399093 75 022399094 76 022399095 77 022399096 78 022399097 79 022399098 80 022399099 81 022399100 82 022399101 83 022399102 84 022399103 85 022399104 86 022399105 87 022399106 88 022399107 89 022399108 Saaplalist Text MeOH Blank MaOH Blank Tox 120, RBL02229-H2O Blk-1, 2/22/99 Tox 120. RBL02229-RBLivarBlk-l. 2/22/99 Tox 120, RBL02229-5ppb-l, 2/22/99 Tox 120, RBL02229-10ppb-l, 2/22/99 Tox 120. RBM2229-25ppb-l, 2/22/99 Tox 120, R8L02229-50ppb-l, 2/22/99 Tox 120, RBL02229-100ppb-l, 2/22/99 Tox 120, RBU>2229-250ppb-l, 2/22/99 Tox 120, RBL02229-500ppb-l, 2/22/99 Tox 120, RBL02229-750ppb-l, 2/22/99 Tox 120, RBL02229-1000ppb-l, 2/22/99 MeOH Blank TOX 120 DNO. 97F-G1. 2/22/99 TOX 120 DNO. 98F-G1, 2/22/99 Tox 120 DUO. 99P-G1. 2/22/99 Tox 120 DUO. 10F-C1, 2/22/99 Tox 120 DMO. 10IF-Cl, 2/22/99 Tox 120, RBL02229-250ppb-CCl, 2/22/99 MeOH Blank Tox 120 DNO. 102F-G1, 2/22/99 Tox 120 DMO. 103F-G1. 2/22/99 Tox 120 DNO. 104F-G1, 2/22/99 Tox 120 DNO. 105F-C1, 2/22/99 Tox 120 DNO. 106F-G1, 2/22/99 Tox 120, RBX,02229-250ppb-CC2, 2/22/99 MeOH Blank Tox 120 DNO. 107F-G1, 2/22/99 Tox 120 DNO. 109F-G1,.2/22/99 Tox 120 DNO. 110F-G1, 2/22/99 Tox 120 DNO. 11F-G1, 2/22/99 Tox 120, RBL02229-250ppb-CC3, 2/22/99 MeOH Blank ' T ox 120 DNO. 113F-G2, 2/22/99 Tox 120 DNO. 114F-G2, 2/22/99 Tox 120 DNO. 115F-G2, 2/22/99 Tox 120 ONO. 116F-G2. 2/22/99 TOX 120 DNO. 117F-G2. 2/22/99 Tox 120, RBL02229-250ppb-CC4. 2/22/99 MaOH Blank Tox 120 DNO. 118F-C2. 2/22/99 Tox 120 DNO, 119P-G2, 2/22/99 Tox 120 DNO. 120F-G2, 2/22/99 Tox 120 DNO. 121F-G2. 2/22/99 TOX 120 DNO. 122F-Q2, 2/22/99 Tox 120, RBL02229-2S0ppb-CC5, 2/22/99 MaOH Blank Tox 120 DNO. 123F-G2, 2/22/99 Tox 120 DNO. 125F-G2, 2/22/99 Tox 120 DNO. 126F-G2, 2/22/99 Tox 120 DNO. 127F-G2. 2/22/99 Tox 120 DNO. 129F-G3, 2/22/99 TOX 120, RBL02229-250ppb~CC6, 2/22/99 MaOH Blank Tox 120 DUO. 130P-C3, 2/22/99 TOX 120 DNO. 131F-G3, 2/22/99 Tox 120 DNO. 132F-G3, 2/22/99 'Tox 120 DNO. 133F-G3, 2/22/99 Tox 120 DNO. 134F-G3, 2/22/99 Tox 120, RBL02229-250ppb-CC7, 2/22/99 MeOH Blank . Tox 120 DNO. 13SF-G3, 2/22/99 Tox 120 DtyO. 136P-C3, 2/22/99 'Tox 120 DNO. 137F-C3, 2/22/99 Tox 120 DNO. 138F-G3, 2/22/99 Tox 120 DNO. 139F-C3, 2/22/99 Tox 120, *BL02229-250ppb-CC8, 2/22/99 MeOH Blank Tox 120 DNO. 141P-G3, 2/22/99 Tox 120 DNO. 142F-G3, 2/22/99 Tox 120 DNO. 143F-G3, 2/22/99 Tox 120, *BL02229-250ppb-CC9, 2/22/99 MaOH Blank Tox 120, 106F-Gl-250ppb-KS, 2/22/99 Tox 120, 106F-Gl-250ppb-MSD, 2/22/99 Tox 120, RBL02229-250ppb-CC10, 2/22/99 MaOH Blank Tox 120, RBL02229-H2O Blk-2, 2/22/99 Tox 120, RBL02229-RBLiverBlk-l, 2/22/99 Tox 120, RBL02229-5ppb-2, 2/22/99 Tox 120, RBl02229-10ppb-2, 2/22/99 Tox 120, RBL02229-25ppb-2, 2/22/99 Tox 120, XfiL02229-50ppb-2, 2/22/99 Tox 120, RBL02229-100ppb-2, 2/22/99 Tax 120, R8L0229-2SOppb>2, 2/22/ Tox 120, RBL02229-500ppb-2, 2/22/99 Tox 120, RBL02229-7S0ppb-2, 2/22/99 Tox 120, RBLO2229-000ppb-2, 2/22/99 Sag 2 Type Analyte Analyte Analyte Analyte Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte QC Analyte Analyte Analyte Standard Standard Standard Standard Standard Standard Standard Standard Standard ` ' . . Std Cono XT 5.00 10.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00 6.119 6.121 6.812 6.819 6.818 6.820 6.734 6.120 6-735 4.117 6.905 250.00 7.595 6.644 6.651 250.00 7.594 6.650 6.565 250.00 6.651 7.507 250.00 250.00 6.565 6.735 7.596 6.652 6.559 250.00 6.651 250.00 6.649 6.651 .6^564 7.419 250.00 250.00 250.00 6.564 6.565 7.597 7.505 6.564 .650 .455 6.455 5.00 10.00 25.00 50.00 100.00 250.00 500.00 750.00 1000.00 6.639 6.565 6.563 6.564 6.561 6.565 6.562 6.560 6.614 Area cone. %Dev Flags 240 67 301 545 1014 2165 5727 10773 15322 20746 254 3.60 24.13 6.46 17.91 39.86 93.78 260.71 497.13 710.29 964.42 MM 383 MM -35 bb -28 MM -20 MM 6 MM 4 US -1 MM -5 MMX -4 MMX 4.25 MM * 60 190 4553 0.00 bb 34.06 MM 205.70 -1 MM \ 1 1 .72 * 0.00 654 23.01 bb bb 3582 160.19 -36 MM 1 1 1 4933 223.51 -11 M M I 102 3329 103 34 92 3818 4340 248 125 3744 0.00 bb 148.34 -41 MM 0.00 0.00 0.00 171.27 m 101 -31 MM j. 195.72 -22 MM 3.96 0.00 167.80 ~M M KM -33 M K / / .. \ / \ _,, \q \ (N __ 1 p i , \ ss. I 1 l V tri/.,. fa r1 '' r t n a 11 t o , II < 1l1 oi 1 I 80 0.00 bb 1 100 3096 59 59 2805 3249 1652 2075 . 0.00 MM 137.40 -45 M K bb0.00 . bb 0.00 123.80 -50 M M 144.61 MM 69.77 MM 89.60 -64 MM 1 JI 1 / / / 130 207 452 925 1701 3784 7436 10539 15696 0.00 -100 MMX 2.05 -79 MMX 13.54 -46 MMX 35.68 -29 KMX 72.05 -28 MMX 169.67 -32 MMX 340.79 -32 MMX 486.19 -35 MMX 727.78 -27 MMX Soup 020199:: quantitated and printad by naa 2/25/99 Soup 0 2 0 1 9 9 :Xquantitatad and prlntad by SSSSaSSSSSiiSrSasiSSSSSasaSSgSSSSSSaBBSSSSSiaSaSiiSSiiSKKSiiBiiSKiitKKStiKiiSSKSUKSCSSS. Mii iiiii iiiii lilliilllillilililliliiiiillililiiliililliliiiiliilliilllliii! ilili iiiii mmntmmmmmmmmmmnimmimmiminmmimmimnmmii Efig s s g s s S g g S g B S g g B S g S S s S g g g g S B g S S S s S B g g S S g g B S g S g g g s S g g g g g s g g g S S s g S g S g g B g S S g S g B S g g S g g g s - S S ss: I P ! P Siili SS5S i S 8 * s i s c 5 s 2129199 laislsssu. 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SSS55 3SS5S3 SgSgpK 3SSSSS S3SSS3 S3SS5S. 53 3 K 33S as SS SR SS SS a SSSg335&33 3333K sSS 838988 333588 8385 *32 3*3S538853 H a s p s i s i s a s a i s s i s a s ? s a l s a s spassi: sai! s a l k s s s s s s s s p p s 55353555535 SSSS53 35535 3533 333353 353553 p p p P P P p p p p p S35 38333533333 h f* I> I M 1* M 1* h r - r- r- i> i> r*>r* r* r r* r- r* r* p* t* f* h* r* r* r~ r* r* r* r* i* r* i* r* h h h r* h p- r-* r- r*- r* r* f - r f r* r* r* r~ sssssssss sssssssss s s ss sS s s s s N s s s s s s sO, s s sssssssss sssssssss !i S :5 > t Si it t i bss'5i5l? 555551 55551 5555'j 5j 5555'J 5Jjj 5ff55l 555'f lit I ,, k i l l s u n i i i i i i i i n n i n i n i n u n i n i =" n m i jjjg i i i i f i i i 1?l3aSlallS|gg|ggls8iggiaag8ggi|gigggSagiiig|igig|a3igggiigifgg?iiissg|aaSgSaaSa 333353'33S'S'3'533353*S33S3333333S3S3SS3S38SS33''3SS333i5S8333;3333S33333555;3S33S333333 11222222222221222222122222212222212222221222222122221212222221222222122225222122222222222 : iiiiiiiiiiilii i iliiiiliiiiii Sf iis l i i l l i fiiSsSsSsSsSsSsSlSsSf 'ssssssasaasssssssssssssssssRSssaaasaassassKKSSSKSssasassssasppppppspssssassssssa Quantify Calibration Report ton 120 OHO Hat Liver Sxtraeta Calibration* C:\MM8LYia\rACT-l.FllO\CumDa\022399a Last aodifiadx Thu F*b 23 13:43x28 1999 printed! Thu Fab 25 13x43x55 1999 . BACK TO MAIN 1 . Soup 020199xx qpantltated and printed by nee 2/25/99 Quantify Calibration Haport V o x 130 0N0# Sat Livar Xxtracta Calibration! C:\XMSLntX\FACT-l.P1t0\CurTaO9\022399o L u t nodiflad: Thu Tab 25 13:43:29 1999 Print ad: Thu Fab 25 13:43:55 1999 1 ] ] 1 1 1 I 1 Ii j J 1 ] ] 1 Soup 020199it Quantitatad and printad by naa 2/25/99 ' BACK TO MAIN . pAffa 2 1 ___________________ _ I Quantily Calibrati xaport TO* 120 W O , Rat L l n r Kxtracta Calibr a t i m i ci \M99una\ncr-l .mo\CarraBB\022399e l u t nsdlflads Thu rb 25 13:43:2t 1995 rrlntad) Thu Tmh 35 13:41:55 1999 ] 1 ] 1 1 j J 3 ] I I i I Soup 020199: x qu*ntltfttd mn4 prlnttd b y m 2/25/99 BACK TO MAIN Quantify Calibrati* Haport Tox 120 OMO, Hat Librar Extracta Cai ibrat ioni C:\NA5flLTn\PaCT-l.PlO\CamDB\022399o U a t aodifiadi Thu Pab 25 13:43*20 1999 Prlntad: Tfau Pab 25 13:43:55 1999 BACK TO MAIN Papa 4 , Soup 020199:: Quantitatad and prlntad by aaa 2/25/99 Quaatify Calibrati Itaport To 120 XMO# ftat Livar Xxtraeta . C a l i b r a t i m i C:\KiMLinoc\FACT-l.Fio\CarraDS\032399o Last aodlfiadi Ttou Fato 25 13143:2* 1999 Frlatodi ni Fb 29 13141153 1919 BACK TO MAIN - Pag S _ Quantlfy Calibrati lapert m 120 EMO Hat Lirar Extracts Calibratimi ci\maunoc\nc*>l.mo\ciirran\022399e L u t aodifladi m fab 25 13t43i3* 1* rrintadt m u rab 25 13i<3iS3 1* BACK TO MAIN oup 020X99H uaatltatad and prlafead by 2/25/99 Method Report Method file* Last modified* Compound Printed C \MASSLYHX\FACT-1. VftO\MethDS\022399c.mdb ttm Feb 33 133514 199* 1 pros Wed Kar 03 16i4129 1999 Incarnai M t . i Quantify Tracai Cone, of Standard*i Uaar *F Valuai Usar Peek Faeton Intagrato Window Faeton [Nona] TIC 11..000000000000, Disabled 3.000 Hatanti on Tina (nina)i Kolativa Retention Tlnat Tin* Window (mina) i Peak Selectioni ft.03. enabled 0.000, Dieablad 1.030 Response Type Polynomial Type 3 Point of Origin Fit Weighting: Axis Transformationi 1 Peak-to-peak amplitude Smoothingi Window site (scans) H u b e r of smooths Smoothing method D1isabled a Join valley threshold Reduce peak tailing Raise baseline maximum Draw vertical threshold Detect shoulderst Shoulder threshold 30.00 70.00 4.00 10.00 ' Disabled 10.00 Relative height Absolute height Relative area Absolut* area 30.00, Enabled 0.00. Disabled 3.00, b a b i e d 0.00. Disabled BACK TO MAIN - , e>2bl<M i; U r . Dll<o3/qq Page 1 I BACK TO MAIN SOUP 020199: FACT Equipment Log 99014 Quattro II,#6414E PAGE DATE: 2/24/99 .. ANALYST: drb . ] STUDY NUMBER:Tox-120 MATRIX: R at Liver DESCRIPTION: Liver Samples . ] FACTM ethod: FACT-M-4.0 . . Injection volume: 10 uL Cycle tim e: 13.5 min. Electrode: Z-Sprav_______ J M obile Phase: (A12.0 mM NH4CH3CQ2 . (BVMeOH______________ TN-A- or stndxd number (A) 98012-1-4 ffi) TN-A-2260 1 Prepared (date/by): /A) 2/11/99 hoi___ (E l 2/99. hoi Expires: (A) 8/99 (BV 8/99_______________ . Stoip at room temp? |5] or N O th er___________________ ;_____ 1 G uard colum n: None________________ __ P art num ber na______ _ Lot number: n a ____________ 1 A nalytical colum n: Keystone Betasil__________________________________:____ I Size: 2 x 100 mm_______________ Type: C18____________ Sphere size: 5 um_________ Jt , '. J P art number 105-701-2__________ Lot number: P8C09 Serial num ber 10881106D A nalytical param eters: . Drying gas (L/hrl: 300 ESI nebulizing gas (L/hrl 15 Sheath gas fL/hrl 0 J LC Flow rate: 300 uL/min. Backpressure: 120 bar ' H PLC solvent gradient: Long 2 Time fm in.l JbB________ 1)JLQQ___ ___ m Q _________ ! 2) 8.50 90.0 ! 3) 11.0 J(LQ__________ . 4) 12.0_________ 40.0 ________ 5) 13.5 . Stop__________ I BACK TO MAIN Tuning Method Report Method : C :\MAS SLYNX \FACT -1. PRO\ACQUDB\PFOS Printed: Wed Feb 24 15:22:59 1999 Spuj> Q 1 0 W IA<L 05/to f o Page 1 1 3 1 I ! { MS I SOURCE ( ESP- ) j Capillary . Cone . Extractor I RF Lens J Source Block Temp. Desolvation Temp. ] j Pressures . J Analyser Vacuum Gas Cell Set Rdbk 3.00 60 5 0.2 150 250 2.99 -60 -7 .150 250 Rdbk 1.2e-4 5.2e-3 MSI . LM Resolution HM Resolution Ion Energy Ion Energy Ramp ' Lens 6 Multiplier . " .*v MS2 LM' Resolution HM Resolution Collision. Ion Energy Ion Energy Ramp Lens 8 . Lens 9 Multiplier Set Rdbk 14.0 14.0, 2.0 q . 0.0 53 650 Set Rdbk 15.0 15.0 45 42 2.0 0.0 40 38 .0 42 650 Solvent Delay ( nina ) I 0.00 Function t 1 MEM of 3 Mass Fairs ( W - ) - Inter Channel Delay ( Secs ) * Span { Daltons ) t ' Start Tie ( Mins ) t End Tine ( Mins ) i Repeats * Channel Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell (Secs) Coll Energy (eV) Cone ( V.) 399.00 80.00 399.00 99.00 399.00 130.00 0.25 0.25 0.25 45 45 45 60 60 60 Function i 2 Iof 3 Mass pairs (M i - ) 3 Inter Channel Delay ( Secs ) t Span ( Daltons ) > Start Tine { Mins ) Cnd Tine ( Mins ) t 0.03 0.00 0.00 13.50 * * Repeats i 1. Channel Parent Daughter Dwell (Secs) Coll Knergy (eV) Cone ( V ) 499.00 80.00 0.25 45 60 1 499.00 99.00 499.00 130.00 0.25 0.25 45 45 60 60 Function i 3 MRX of 2 Mass Pairs ( M l - ) 1 Inter Channel Delay ( Secs ) i 0.03 Span ( Daltons ) i 0.00 Start Tine ( Kins ) * 0.00 . End Tine ( Kins ) .i 13.50 Repeats i 1 Channel Parent Daughter Dwell (Secs) Coll Energy (eV) Cone ( V ) 299.00 80.00 299.00 99.00 0 .2& 0.25 45 45 35 35 Function i 4 MEM of 3 Mass Pairs ( ESP- ) Inter Channel Delay ( Secs } i Span ( Daltons ) t Start Tine ( Mins ) t * End Tine ( Kins ) s Repeats t Channel Parent Daughter 0.03 0.0Q 0.00 13.50 1 Dwell (Secs) Coll Energy (eV) . Cone ( V ) 413.00 119.00 413.00 169.00 413.00 219.00 0.25 0.25 0.25 30 20 30 15 15 15 J Function i 5 MEM of 3 Mass Pairs ( ESP- ) Inter Channel Delay ( Sees ) s 0.03 Span ( Daltons ) i 1 0.00 Start Tina ( Mina ) t * 0.00 End Tins ( Mina ) t 13.50 I Repasts i 1. Channe l Parent Daughter Dwell (Secs) Coll Energy (eV) Cone ( V ) 469.00 169.00 0.25 45 30 469.00 219.00 0.25 45 30 469.00 119.00 0.25 45 30 Function t 6 MSM of 3 Mass Fairs ( SSF- ) later Channel Delay ( Secs ) * Span ( Daltons ) t Start Tine ( Mins ) i ' End Tine ( Mina ) j Repeats t * Channel Parent Daughter 569.00 169.00 569.00 219.00 569.00 119.00 0.03 0.00 0.00 13.50 1 Dwell (Seca) Coll Energy (eV) 0.25 0.25 0.25 45 45 45 Cone ( V ) 35 35 35 Function t ? MEM of 1 Mass Fair ( ESP- ) Zntsr Channel Delay ( Secs ) t 0.03 Span ( Daltons ) j 0.00 * Start Tins ( Kins ) t End Tine ( Kins ) * 0.00 13.50 . Repeats t *1 Channel Parent Daughter Dwell (Secs) Coll Energy (eV) Cone ( V ) 427.00 80.00 0.25 40 60 . BACK TO MAIN $ BACK TO MAIN M asslynx - Sam ple List Sam ple List: C:\MASSLYNX\FACT-1 .PRO \Sam pleDB\022499a.SPL Printed: W ed Feb 24 15:25:391999 ,, . /, I j . _____________________________ W p m o m ___ lAC.QS/lolri, 0U >. co ' Page 1 F ile Nam e File Text M S File In le tl Botti olum e Sam ple T j C one A C o n e B C o n c C 1 022499001 MeOH Blank TOX12C long2 91 10 Analyte 2 022499002 MeOH Blank TOX12C long2 91 10 Analyte 3 022499003 Tox 120, RBL02229-H2O B lk -1 ,2/22 TOX12C long2 1 io Analyte 4 022499004 Tox 120, RBL02229-RBUverBlk-1, 2. TOX12C long2 2 10 Analyte 5 022499005 Tox 120, R BL02229-5ppb-1,2/22/99 TOX12C long2 3 10 Standard ' 6 022499006 Tox 120, RBL02229-1 Oppb-1,2 /2 2 /9 TOX12C long2 4 10 Standard 7 022499007 Tox 120, RBL02229-25ppb-1,2 /2 2 /9 TOX12C long2 5 . 10 Standard 8 022499008 Tox 120, RBL02229-50ppb-1,2/22/9 TOX12C long2 6 10 Standard 9 022499009 Tox 120, RBL02229-100ppb-1,2 /2 2 / TOX12C long2 7 10 Standard 10 022499010 Tox 120, RBL02229-250ppb-1,2 /2 2 / TOX12C long2 8 ' 10 Standard . 11 022499011 Tox 120, RBL02229-500ppb-1,2 /22 / TOX12C long2 9 10 Standard 12 022499012 Tox 120, RBL02229-750ppb-1.2 /22 / TOX12C long2 10 10 Standard 13 022499013 Tox 120, RBL02229-1000ppb-1,2 /2 2 TOX12C long2 11 10 Standard 14 022499014 MeOH Blank . TOX12C long2 92 10 Analyte 15 022499015 Tox 120 DNO. 145F-G 4,2/22/99 ` TOX12C long2 12 10 Analyte . - 16 022499016 Tox 120 DNO. 146F-G 4,2/22/99 TOX12C long2 13 10 Analyte 17 022499017 Tox 120 DNO. 147F-G 4,2/22/99 TOX12C long2 14 10 Analyte 18 022499018 Tox 120 DNO. 148F-G 4,2/22/99 TOX12C long2 15 ' 10 Analyte 19 022499019 Tox 120 DNO. 4 9 F -G 4 ,2/22/99 TOX12C long2 16 10 Analyte 20 022499020 Tox 120, RBL02229-250ppb-CC11, TOX12C long2 17 10 QC 21 022499021 MeOH Blank TOX12C long2 92 10 Analyte 22 022499022 Tox 120 DNO. 150F-G 4,2/22/99 TOX12C long2 18 10 Analyte 23 022499023 Tox 120 DNO. 151F-G 4,2/22/99 TOX12C long2 19 10 Analyte 24 022499024 Tox 120 DNO. 152F-G 4,2/22/99 TOX12C long2 20 10 Analyte 25 022499025 Tox 120 DNO. 1 53F -G 4,2/22/99 TOX12C long2 21 10 Analyte 26 022499026 Tox 120 DNO. 1 54F -G 4,2/22/99 TOX12C long2 22 10 Analyte 27 022499027 Tox 120, RBL02229-250ppb-CC12, i TOX.12C long2 23 10 QC 28 02249928 MeOH Blank TOX12C long2 92 10 Analyte 29 022499029 Tox 120 DNO. 155F-G 4,2/22/99 TOX12C long2 24 10 Analyte 30 022499030 Tox 120 DNO. 157F-G 4,2/22/99 TOX12C long2 25 10 Analyte 31 022499031 Tox 120 DNO. 158F-G 4,2/22/99 TOX12C long2 27 10 Analyte 32 022499032 Tox 120 DNO. 159F -G 4,2/22/99 TOX12C long2 28 10 Analyte . 33 022499033 Tox 120, RBL02229-250ppb-CC13, TOX12C long2 29 10 QC . 34 022499034 MeOH Blank TOX12C long2 93 10 Analyte 35 022499035 Tox 120 DNO. 161F-G 5,2/22/99 36 022499036 T o x 120 D N O . 162F-G5, 2/22/99 TOX12C long2 30 T O X 1 2 C long2 31 10 Analyte 10 Analyte 37 022499037 Tox 120 DNO. 163F-G 5,2/22/99 TOX12C long2 32 10 Analyte 38 022499038 Tox 120 DNO. 164F-G 5,2/22/99 TOX12C long2 33 10 Analyte 39 022499039 Tox 120 DNO. 165F-G 5,2/22/99 TOX12C long2 34 10 Analyte 40 022499040 Tox 120, RBL02229-250ppb*CC14, i TOX12C long2 35 10. QC 41 022499041 MeOH Blank TOX12C iong2 93 10 Analyte 42 022499042 Tox 120 DN. 166F-G 5,2/22/99 TOX12C long2 36 10 Analyte ' 43 022499043 T o x `120 DNO. 167F-G 5,2/22/99 TOX12C long2 37 10 Analyte 44 022499044 Tox 120 DNO. 168F-G 5,2/22/99 TOX12C long2 38 10 Analyte 45 022499045 Tox 120 DNO. 16 9F -G 5,2/22/99 TOX12C long2 39 10 Analyte 46 022499046 Tox 120 DNO. 170F-G5 2/22/99 TOX12C long2 40 10 Analyte 47 022499047 Tox 120, RBL02229-250ppb*CC15, i TOX12C long2 41 10 QC . ` `48 022499048 MeOH Blank TOX12C long2 93 10 Analyte 49 022499049 Tox 120 DNO. 171F-G5 2/22/99 TOX12C long2 42 10 Analyte 50 022499050 Tox 120 DNO. 173F-G5 2/22/99 TOX12C long2 43 10 Analyte 51 022499051 Tox 120 DNO. 174F-G5 2/22/99 TOX12C long2 44 10 Analyte 52 022499052 Tox 120 DNO. 175F-G5 2/22/99 TOX12C long2 45 10 Analyte 53 022499053 Tox 120 DNO. 177F-G6 2/22/99 TOX12C long2 46 10 Analyte 54 022499054 TOX 120, RBL02229-250ppb-CC16, i TOX12C long2 47 10 QC 55 022499055 MeOH Blank TOX12C long2 94 10 Analyte , 56 022499056 Tox 120 DNO. 178F-G6 2/22/99 TOX12C long2 48 10 Analyte ' 57 022499057 Tox 120 DNO. 179F-G6 2/22/99 TOX12C long2 49 10 Analyte 58 022499058 Tox 120 DNO. 180F-G6 2/22/99 TOX12C long2 50 10 Analyte 59 022499059 Tox 120 DNO. 181F-G6 2/22/99 TOX12C long2 51 10 Analyte BACK TO MAIN M asslynx Sam ple List Sam ple List: C:\MASSLYNX\FACT-1.PRO\SampleDB\022499a.SPL Printed: W ed F eb 2 415:25:391999 r Soup 0 1 0 m . . . _ laA lA^aSllojlf / . Page 2 File Nam e R ie Text M S File In let I Botti olum e Sam ple T j C one A C one B C one C 60 022499060 Tox 120 DNO. 182F-G6 2/22/99 TOX12C long2 52 61 022499061 Tox 120, RBL02229-250ppb-CC17, TOX12C long2 53 62 022499062 MeOH Blank TOX12C long2 94 63 022499063 Tox 120 DNO. 183F-G6 2/22/99 TOX12C long2 54 64 022499064 Tox 120 DNO. 84F -G 6 2/22/99 TOX12C long2 55 65 022499065 Tox 120 DNO. 185F-G6 2/22/99 TOX12C long2 56 66 022499066 Tox 120 DNO. 186F-G6 2/22/99 TOX12C long2 57 67 022499067 Tox 120 DNO. 187F-G6 2/22/99 TOX12C long2 58 68 022499068 Tox 120, RBL02229-250ppb-CC18, ! TOX12C long2 5 9 . 69 022499069 MeOH Blank ' TOX12C long2 95 7 0 022499070 Tox t2 0 DNO. 189F-G6 2/22/99 TOX12C long2 60 71 022499071 Tox 120 DNO. 190F-G6 2/22/99 TOX12C long2 61 72 022499072 Tox 120 DNO. 191F-G6 2/22/99 . TOX12C long2 62 7 3 022499073 Tox 120, RBL02229-250ppb-CC1,2 / TOX12C long2 63 7 4 022499074 MeOH Blank TOX12C long2 95 7 5 022499075 Tox 1 2 0 ,110F-G1-250ppb-MS, 2/22/ TOX12C long2 64 76 022499076 Tox 1 2 0 ,1 10F-G1-250ppb-MSD, 2/2 TOX12C long2 65 7 7 022499077 Tox 120, RBL02229-250ppb-CC2,2/ TOX12C long2 66 78 022499078 MeOH Blank TOX12C long2 95 79 022499079 Tox 120, RBL02229-H2O B lk-2,2/22 TOX12C long2 67 80 022499080 Tox 120, RBL02229-RBLiverBlk-1,2. TOX12C long2 68 81 022499081 Tox 120, RBL02229-5ppb-2,2/22/99 TOX12C long2 69 82 022499082 Tox 120, RBL02229-10ppb-2,2/22/9 TOX12C long2 70 83 022499083 Tax 120, RBL02229-25ppb-2,2/22/9 TOX12C long2 71 84 022499084 Tox 120, RBL02229-50ppb-2,2/22/9 TOX12C long2 72 8 5 022499085 Tox 120, RBL02229-100ppb-2,2/22/ TOX12C long2 73 86 022499086 Tox 120, RBL02229-250ppb-2,2/22/ TOX12C fong2 74 8 7 02249987 Tox 120, RBL02229-500ppb-2,2/22/ TOX12C long2 75 88 022499088 Tox 120, RBL02229-750ppb-2,2/22/ TOX12C long2 TO SS 022499089 Tox 120, RBL02229-1000ppb-2,2/22 TOX12C long2 77 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte . 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 1 0. Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN Date: c j c n h Analyst: , FACT StudyNumber ^ J L \2 Q Matrix Z vSrJA Time Points yfgMjxi \o Dilutions? (vJ____ Analyte 1: Pfcs Acceptable TornearRange: '/ / c s^p p t) LOQ for Ibis data set Re-evaluated for differentrange? A) Analyte 4: n5n Acceptable Linear Rang e : / ^ 0 ~~/ , LOQ for this data set / ^ o Re-evaluated for.different range? Analyte 5: Acceptable LinearRan g e:/O -- ' LOQ for this data set 1 Re-evaluated for differentrange? Anafyte2: Acceptable Linear Range: u.rv> oU ^ U y f a i&k, r + ^ 'C c T T c o r r * X ^ 3 r \ - s ^ LOQ for tf set t o A f-'OjL*~v Re-evaluated far different range? ________ Analyte 3: roAA Acceptable Linear Range: LOQ for this data s e t c P -tS ^ a p ^ Re-evaluated for different range? fOo 1 - :i L___ ss5 sssssssss^ ^c:d ;ss5 S 5 2 2 S P g aa3 S S 2 ;;!;ss5 S 5 S ssst;:si:K ;sS s;s;;J2 sssJss:ccts --i,,u .fcuMM- m n h n m h m n h j m h j m i ^ m i ^ n m!g ; ; ^ ; i i i g ; s i i i s E ; g i i i i E ; i i i g g ; i i i | g ' 8 | g i i g ' 8 i i g ; i i g g E ; i i g i i E ; ; ; ; ; ; s ; ; ; ; g E : I n i l iiiifi m u i f i n i ! f i m i m u l i n n iiff ff : g I *\ i( Sa-asasc.. ssssssssa aa ss 8 s SSSSbSs Sb s s s s b s SEs b SbbbSb^^^ a a a a a gaaaSsas s s s s s ssssssssa g g g g g g g g g g g g g ^ g g -i SSSSS SHSSS S s s s S " g a s a s s SSbss " s SSbsbbskE Es llg5s=lIlIsc!lll3=lSE M M SS5 llSS Jslsl llSI 3 g SS B M M M MM H M M M W H H H M M M H H H M H H EEsaasijiCu.-o.SEsBSiiaaSsssassasllSKssKsa; Essass asHssi Essa S^sSs alSasa sSasEsssrs.; ,,s albsaisgsssbsBbbibsbrSsr: as:-.":rrs3"sKs sbsa atabas sabss ssssbb bbbbss bbabssbssss s? gSBBSSSggmsmgBSBSBgBBBgBBBBBBgBBSgBBB SSSBSS SBBBSB BSBBB BBBSBB BBB8SE SSSSSSSggBS S? BACK TO MAIN Quantify Coneound Susmary Mport TCX-120 Rat Livar larnpli List: C: \HASSLTncc\7ACT-l.?RO\aleDl\Oa2499a Last modified: W ad Mar 10 09:21:55 1*99 Method: Ct \MAS8L7MX\7ACT-l.fltO\HatbDS\022499a Last nodlfladx Tua Mar 09 13:53:43 1999 Job Coda: Priatadi W ad Mar 10 09:43:5 1999 Compound 2: * n i Mama 1 022499001 2 022499002 3 022499003 4 022499004 5 022499005 022499006 7 022499007 8 022499008 9 022499009 10 022499010 11 022499011 12 022499012 13 022499013 14 022499014 15 022499015 16 022499016 17 022499017 18 022499018 19 022499019 20 022499020 21 022499021 22 022499022 23 022499023 24 022499024 25 022499025 26 022499026 27 022499027 28 022499028 29 022499029 30 022499030 31 022499031 32 022499032 33 022499033 34 022499034 35 022499035 36 022499036 37 022499037 38 022499038 39 022499039 40 022499040 41 022499041 42 022499042 43 022499043 44 022499044 45 022499045 46 022499046 47 022499047 48 022499048 49 022499049 50 022499050 51 022499051 52 022499052 53 022499053 54 022499054 55 022499055 56 022499056 57 022499057 58 022499058 59 022499059 60 022499060 61 022499061 62 022499062 63 022499063 64 022499064 65 022499065 66 022499066 67 022499067 68 022499068 69 022499069 70 022499070 71 022499071 7^ 022499072 73 022499073 74 022499074 75 022499075 76 022499076 77 022499077 78 022499078 79 022499079 80 022499080 81 022499081 82 022499082 83 022499083 84 022499084 85 022499085 86 022499086 87 022499087 88 022499088 89 022499089 Sai^lallst Taxt MeOK Blank * MaOH Blank Tox 120 , RBL02229-H2O Blk-1. 2/22/99 Tox 120 . RBL02229-RBUverBlk-l, 2/22/99 Tox 120 , RBL02229-5ppb-l, 2/22/99 Tox 120 , R5L02229-10ppb-l, 2/22/99 Tox 120 . RBL02229-25ppb-l, 2/22/99 Tox 120 , RBL02229-50ppb-l, 2/22/99 Tox 120 , RBLQ2229-100ppb-l, 2/22/99 Tox 120 , RBL02229-250ppb-l, 2/22/99 Tox 120 , RBL02229-500ppb-l, 2/22/99 . Tox 120 , RBLO2229-750ppb-l, 2/22/99 Tox 120 . RBL02229-1000ppb-l, 2/22/99 MaOH Blank Tox 120 DUO. 145P-G4. 2/22/99 . Tox 120 DUO. 1467-64, 2/22/99 Tox 120 DUO. 1477-64, 2/22/99 Tox 120 DMO. 1487-64, 2/22/99 Tox 120 DMO. 1497-64, 2/22/99 Tox 120,, RBL02229-25Oppb-CCll, 2/22/99 MaOH Blank Tox 120 DMO. 1507-64, 2/22/99 Tox 120 DMO. 1517-64, 2/22/99 Tox 120 DMO. 1527-64, 2/22/99 Tox 120 DMO. 1537-64; 2/22/99 Tax 120 DNO. 1547-64, 2/22/99 Tox 120,r RBL02229-250ppb-CC12, 2/22/99 MaOH Blank Tox 120 DMO. 1557-64, 2/22/99 T ox 120 DMO. 1577-64, 2/22/99 Tox 120 DNO. 1587-64, 2/22/99 Tox 120 DNO. 1597-64, 2/22/99 Tox 120, ABL02229-250ppb-CC13, 2/22/99 MaOH Blank Tox 120 DNO. 1617-G5, 2/22/99 Tox 120 DNO. 1627-G5, 2/22/99 Tox 120 DNO. 1637-C5, 2/22/99 Tox 120 DNO. 1647-G5, 2/22/99 Tox 120 DNO. 1657-G5. 2/22/99 T ox 120, RBL02229-250ppb-CC14, 2/22/99 MaOH Blank Tox 120 DNO. 1667-G5, 2/22/99 Tox 120 DNO. 1677-G5, 2/22/99 Tox 120 DNO. 1687-G5, 2/22/99 Tox 120 DNO. 1697-G5, 2/22/99 Tox 120 DNO. 1707-G5 2/22/99 Tox 120, RBL02229-250ppb-CC15, 2/22/99 MaOH Blank Tox 120 DNO. 1717-C5 2/22/99 Tox 120 DNO. 1737-GS 2/22/99 Tox 120 DNO. 174P-G5 2/22/99 Tox 120 DNO. 1757-G5 2/22/99 Tox 120 DMO. 1777-G6 2/22/99 Tox 120. RBL02229-250ppb-CC16, 2/22/99 MaOH Blank Tox 120 DNO. 1787-G6 2/22/99 T ox 120 DNO. 179P-G6 2/22/99 Tox 120 DNO. 1807-G6 2/22/99 Tox 120 DNO. 1B17-G6 2/22/99 Tox 120 DNO. 1827-G6 2/22/99 * Tox 120, RBL02229-2S0ppb-CC17, 2/22/99 MaOH Blank Tox 120 DNQ. 1837-06 2/22/99 Tox 120 DNO. 1847-06 2/22/99 Tox 120 DNO. 1857-G6 2/22/99 Tox 120. DNO. 1867-G6 2/22/99 Tox 120 DNO. 1877-G6 2/22/99 Tox 120, RBL02229-250ppb-CC18. 2/22/99 MaOH Blank Ton 120 ONO. 189P-C 2/22/99 Tox ,120 DUO. 1907-C4 2/22/99 Tox 120 DUO. 1917-G6 2/22/99 Tox 130, RBL0222-2S0ppb-CCl. 2/32/99 MaOH Blank . Tox 120, 110F-Cl-250ppb-MS, 2/22/99 Tox 120, 110r-Cl-2SOppb-MSS, 2/22/99 Tox 120, UL02229-2S0ppb-CC2, 2/22/99 MaOH Blank Tox 120, RBL02229-H20 Blk-2, 2/22/99 Tox 120, RBL02229-R8LivarBlk-l, 2/22/99 Tox 120, RBL02229-5ppb-2, 2/22/99 Tox 120, RBL02229-10ppb-2, 2/22/99 Tox 120, RBL02229-25ppb-2. 2/22/99 Tox 120, RBL02229-50ppb-2. 2/22/99 Tox 120, RBL02229-100ppb-2, 2/22/99 Tox 120, RBL02229-2S0ppb-2, 2/22/99 Tox 120, RBL02229-S00ppb-2, 2/22/99 Tox 120, RBL02229-750ppb-2, 2/22/99 Tox 120, RBL02229-1000ppb-2, 2/22/99 *ypa Analyta Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyte Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyte Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta- Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Std Cone 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 299.00 299.00 299.00 299.00 299.00 299.00 299.00 299.00 299.00 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 RV 6.648 6.583 6.563 6.562 6.528 6.565 6.539 6.457 6.474 6.465 6.565 7.321 6.464 6.377 6.450 6.465 6.583 6.409 6.389 6.387 6.407 6.542 6.458 6.474 6.476 6.496 6.448 6.490 6.551 6.480 6.459 6.442 6.474 6.451 6.533 6.559 6.441 6.480 6.470 6.448 6.477 6.537 6.461 6.472 .450 6.448 6.466 6.548 6.469 6.472 * 6.411 6.565 6.481 6.376 6.502 6.456 6.512 6.433 6.464 6.481 6.479 6.477 6.477 Araa Dl Cono. 71aas XDmr 157 463 250 314 552 981 2548 4498 7283 8654 428 * 68 36 138 147 1692 173 52 105 1 0.82 MX 1 46.18 MX 1 14.54 MX 1 24.11 bb 1 59.47 bb 1 123.26 MX 1 356.08 MM 1 645.67 MX 1 1059.54 MM 1 1263.23 MX 1 41.08 MX 1 0.00 bb 1 0.00 bb 1 0.00 bb 1 0.00 MM 1 228.87 bb 1 3.11 M M 1 0.00 MM 1. 0.00 M M . 671 21 -19 -1 3 19 8 18 5 -23 1132 1 145.57 MM . -51 C O 1'*' 1354 224055 201712 162554 141519 161586 1657 156 201189 136264 112102 1454 803 1291 109 2497 179 212 222 1435 1317 1670 1445 1256 1196 1402 1548 2042 1376 1 178.69 MM 1 33265.77 MM 1 29946.12 MM 1.24128.42 MM 1 21003.21 MM 1 23984.62 MM 1 223.64 MM 1 0.58 MM 1 29868.45 MM 1 20222.42 MM 1 16632.67 MM 1 193.43 MM 1 96.74 MM 1 169.19 MM 1 0.00 KM 1 348.43 MM 1 4.02 bb 1 8.91 bb 1 10.37 bb 1 190.59 MM 1 173.13 MM 1 225.59 MM 1. 192.01 MM 1 164.01 MM 1 155.12 KM 1 185.75 MM V ^ 207.50 MM 1 280.82 KM 1 181.83 bb -40 \ \ \ -25 4\(7 1ST> J -43 m**-* -42 -31 1603 1 215.57 KM -28 1290 1936 1004 1297. 1 169.12 MM 1 265.13 MX 1 126.59 MM 1 170.13 MM -43 61 82 183 485 887 2050 3785 5715 7311 1 0.00 MX 1 0.00 MX 1 4.70 MM 1 49.55 MM 1 109.18 MX 1 281.95 MX 1 539.86 MX 1 826.54 MX 1 1063.(6 MM -10O -100 -84 -17 -9 -6 -10 -8 -11 Vaga 2 I oi lAC. 03l\0 Soup 020199: Quantitatad on 03/09/99 and prlntad on 03/10/99 by DRB U ? E.' g -f SSC.UHH SCKS -SO ^-' H H H H H H H jp*' s s s Ssk 3SSSSSS y r g is s5sg {.fr ei- r s c . . 8 Oo :: 3*4 2 - ~ . p- -- SuS H S2S 3 555 & T B S' 4 b g SUBIUMH S2S5P332 s 51s S3s . s ssastbisb 5 6 i 3 I 3 IH N ro >o 7 H O > 8.409 8.388 8.388 Soup 020199: Qumneitatad on 03/09/99 and printed on 03/10/99 by DM 333352888322:! ! mESsmsEmSSgmSgtSrsm5=mSSmSm=5EiSstgS=i=gmSgEmgBisSgmE5mg=sig5gmggmBggigSmgS=mggmSBgm=SmESm5gSm=SmSSmsSSmsStgSsmS5tsSsmSSm: ' , g g g r | P P p r , p g g ; g E | p j g | | e si ! i i i > > a i i s a l s i i i > ! i e ! a i i U ) i i a a l ! i*? Si! I SSS5 m 5 s \n m m m m m m m m m m m 8 88888 8 x h h h k k 32*2* 4**2* r *i|ii i !l-ls * v*-*t l Sil?l3 2 5 2 Hh**H * whhH 3 111 I i i SsssSsac.. ssssss'?s sM0 so 3' im aam a M a a m2 a a a M a aa Hw mm aSaO oaU9 a I* aM 333355 sSSSEs 385225 gggggg f 1 1 1M Sss 5 HHH 838 3 33b b ggg g (t9 a 5 H 5 3 g V a H K r 3 a a W 8 l * 5 aH M 8 aaOS Mu-4 . a Ma a HH H I-* H M WM 0 a4 M b s It *u4 aOS It4 M 3 8b a H It ao oa g g. g gg g. g b i a A i HII i a a SHsSsSS,. oooooeoiS aaaaa BESSs l I 38338 HPHHH 33333 38283 ggggg 1 1 1HH aaaaa BACK TO MAIN trcr e^ e -2 5 e 8 c -sim i SSS53S38 naimo9ioi*toi s *1 i i is s s s s s s s s s i s s s " " *3S8Sjj s S.s55 oo i n i nio Mi i ssss m ssssg&g sgss rIHHHHHH s* m u S 5 sss ssssass s ss 55 sssssssss "='" 55555 8 5 NH> II- HHH 5 :s m "1 I I i l l i i l i l i l i l 8 ! l j I ' 1 1 1 . M . , a s 1 g g g g g 1 g g g g g . g g g 1 g g i g g l | g g g i g | g ' | g g l 1 i i g a 1 | i g g l 1 i i i l 1 s l 1 nmmmmHmnmmmnmmmnnmmimnnm^Hn^hmnmn siiliiiiiggSSgsgSssgsgsggssggssssssssssississssisssssssssssissssilsl :s: 3 & S S ili !i !Si Iggoggggg! liiiiii !si 'SSSSSaSSSSSSSSSSSKSSSSSSSSSSSSSgSSSSSSSSSSKSSSSKSSSSSgSSSSSSSgiJPiJiSiepSSSggSSSiSESS i. 8 5 BACK TO MAIN Quantify Compound l u m x y tuport TOX-120 tat Livur c l * Llati C:\M&SSUnoc\MCT-l.mo\fcl.DI\0229S. Last aodifiad: N ad Max 10 0S)21>S5 1090 Mathod: Cl\MUSLK\raCT-l.no\MathI\022499a b u t aodifiad: Tua Mar 09 13:53:43. 1999 Job Coda) rrintad: ' N a d Mar 10 09)43:3 1999 . 1 j _ .1 *1 t -- 53 .VJ **.1 J J 1 COacound ) XI MM aaplallst Taxt Typ Std Cono 1 022499001 KeOH Blank 2 022499002 IteOH Blank Analyta Analyta 3 022499003 Tox 120. RBL02229-H20 Blk-1, 2/22/99 Analyta 5 4 022499004 Tox 120. RBL02229-RBLivar81k-l. 2/22/99 Analyta 022499005 Tox 120. RBL02229-5ppb-l, 2/22/99 Standard 494.00 6 022499006 Tox 120. RBL02229-10ppb-l, 2/22/99 Standard 494.00 7 022499007 Tox 120. RBL02229-25ppb-l. 2/22/99 Standard 494.00 0 022499008 Tox 120. RBL02229-50ppb-l, 2/22/99 Standard 494.00 022499009 Tox 120. RBL02229-100ppb-l, 2/22/99 10 022499010 Tox 120. RBL02229-250ppb-l, 2/22/99 Standard Standard 494.00 494.00 11 022499011 T ox 120,BBL02229-500ppb-l, 2/22/99 Standard 494.00 13 022499012 Tox 120. RBLQ2229-750ppb-l, 2/22/99 Standard 494.00 13 022499013 Tox 120. RBL02229-100Oppb-1 2/22/99 Standard 494.00 1 022499014 IS 022499015 IteOH Blank t o x 120 rara. I45r-c4. 2 /2 2 / 9 9 Analyta Analyta 1 022499016 T ox 120 OHO. 1467-C4, 2/22/99 ' 17 022499017 T ox 120 DUO, 147F-G4. 2/22/99 Analyta Analyta 18 022499018 T OX 120 DNO. 1487-04, 2/22/99 Analyta 19 022499019 T OX 120 DUO. 1497-64. 2/22/99 Analyta 20 022499020 Tox 120. RBL02229-250ppb-CCll. 2/22/99 QC 494.00 21 022499021 MaOH Blank ' Analyta 22 022499022 Tox 120 DNO. 1507-64, 2/22/99 Analyta 23 022499023 TOX 120 DNO. 1517-64. 2/22/99 Analyta 24 022499024 To x 12Q DNO. 1527-64. 2/22/99 Analyta 35 022499025 To x 120 DNO. 1537-64, 2/22/99 Analyta 2 022499026 To x 120 DNO. 1547-64, 2/22/99 Analyta 27 022499027 Tox 120, RBL02229-250ppb-CC12, 2/22/99 QC 494.00 20 022499028 MaOH Blank Analyta 29 022499029 To x 120 DNO. 1557^64. 2/22/99 Analyta 30 022499030 T q x 120 DNO. 1577-64, 2/22/99 Analyta 31 022499031 Tox 120 DNO. 1587-64, 2/22/99 Analyta 33 022499032 Tox 120 DNO. 1597-64. 2/22/99 Analyta 33 022499033 Tox 120. RBL02229-250ppb-CC13, 2/22/99 QC 494.00 34 022499034 MaOH Blank ' Analyta 35 022499035 To x 120 DNO. 1617-65, 2/22/99 Analyta 36 022499036 To x 120 DNO. 1627-65. 2/22/99 Analyta 37 022499037 Tox 120 DNO. 1637-65, 2/22/99 Analyta 38 022499038 TO X 120 DNO. 1647-65, 2/22/99 Analyta 39 022499039 Tox 120 DNO. 1657-65, 2/22/99 Analyta 40 022499040 Tox 120, RBL02229-250ppb-CC14, 2/22/99 QC 494.00 *1 022499041 MaOH Blank 42 022499042 T ox 120 DNO. 1667-65, 2/22/99 Analyta Analyta 43 022499043 Tox 120 DNO. 1677-65, 2/22/99 Analyta 44 022499044 Tox 120 DNO. 1687-65. 2/22/99 Analyta . 45 022499045 T ox 120 DNO. 1697-65, 2/22/99 Analyta . 46 022499046 Tox 120 DNO. 1707-65 2/22/99 Analyta 022499047 Tox 120, RBl*02229-250ppb-CC15, 2/22/99 QC *494.00 40 022499048 MaOH Blank Analyta 49 022499049 Tox 120 DNO. 1717-65 2/22/99 Analyta 50 022499050 TOX 120 DNO. 1737-65 2/22/99 Analyta si 022499051 Tox 120 DNO. 1747-65 2/22/99 . Analyta S2 022499052 Tox 120 DNO. 1757-65 2/22/99 Analyta S3 022499053 To x 120 DNO. 1777-66 2/22/99 Analyta 54 022499054 Tox 120. RB*02229-250ppb--CC16, 2/22/99 QC 55 022499055 MaOH Blank Analyta 494.00 56 022499056- TOX 120 DNO. 1787-66 2/22/99 Analyta 57 022499057 Tox 120 DNO: 1797-66 2/22/99 Analyta SS 022499058 TOX 120 DNO. 1807-66 2/22/99 Analyta SS 022499059 Tox 120 ONO. 1817-66 2/22/99 Analyta 60 022499060 T ox 120 DNO. 1827-66 2/22/99 Analyta 61 022499061 Tox 120, BBL02229-250ppb-CC17, 2/22/99 QC 494.00 63 022499062 MaOH Blank Analyta 63 022499063 Tox 120 DNO. 1837-66 2/22/99 Analyta **s 022499064 022499065 Tox 120 DNO. 1847-66 2/22/99 Tox 120 DNO.* 1857-66 2/22/99 ' Analyta Analyta 6 022499066 Tox 120 DNO. 1867-66 2/22/99 Analyta 67 022499067 Tox 120 DNO. 1877-66 2/22/99 Analyta 68 022499068 Tox 120, RBI.02229-250ppb-CC18, 2/22/99 QC 494.00 69 022499069 MaOH Blank Analyta 70 022499070 TOX 120 DNO. 1897-66 2/22/99 Analyta 022499071 T ox 120 ONO. 1907-66 2/22/99 Analyta 92 022499072 To* 120 DNO. 1917-66 2/22/99 Analyta 'S 022499073 Tox 120, RBM2229-250ppb-CCl, 2/22/99 QC 494.00 74 022499074 MaOH Blank Analyta 75 022499075 Tox 120, 1107-61-250ppb-MS. 2/22/99 Analyta 76 022499076 Tox 120, 1107-61-25Oppb-MSD, 2/22/99 Analyta 77 022499077 Tox 120, RBL02229-250ppb-CC2, 2/22/99 Analyta 7 022499078 MaOH Blank Analyta 79 022499079 Tox 120, HBL02229-K2O Blk-2, 2/22/99 Analyta 0 022499080 Tox 120, RBL02229-RBLivorBlk-l, 2/22/99 Analyta u1 022499081 Tox 120, RBL02229-Sppb-2, 2/22/99 022499082 Tox 120, RBLO2229-10ppb-2, 2/22/99 Standard Standard 494.00 494.00 03 022499083 T ox 120, RBL02229-25ppb-2, 2/22/99 Standard 494.00 as54 022499084 Tox 120, RBL02229-50ppb-2, 2/22/99 022499085 Tox 120, KBta02229-100ppb-2, 2/22/99 Standard Standard 494.00 494.00 06 022499086 Tox 120, KBU)223?-250ppb-2, 2/22/99 Standard 494.00 87 022499087 Tox 120, RBL02229-S00ppb-2, 2/22/99 Standard 494.00 88 022499088 Tox 120. RBD02229-750ppb-2, 2/22/99 Standard 494.00 89 022499089 Tox 120, RBU)2229-1000ppb-2, 2/22/99 Standard 494.00 X 7.314 7.320 7.252 7.236 7.235 7.194 7.237 7.209 7.122 7.145 7.138 7.237 7.136 7.050 7.049 7.116 7.138 7.163 7.082 7.062 7.142 7.031 7.060 7.212 7.142 7.130 7.054 7.072 7.209 7.131 7.144 7.063 7.160 7.119 7.223 7.068 7.130 7.106 7.057 7.124 7.231 7.112 7.151 7.138 7.035 7.145 7.211 7.134 7.059 .7.037 7.035 7.141 7.131 7.142 7.137 7.135 7.143 7.145 7.137 7.068 7.080 6.996 7.152 7.066 7.050 7.208 7.197 7.169 7.122 7.093 7.105 7.136 7.068 7.066 7.064 7.064 oup 020199) Quantitatad on 03/09/99 and pclntad on 03/10/99 by D U A T M Dl .. Cono. Flaga %Dav 348 278 665 847 768 713 583 663 673 673 746 389 * 632 421 472 617 672 329 481 599 580 402 555 316 473 464 362 654 383 676 588 604 515 558 349 497 586 441 35 3 542 340 534 513 493 231 515 236 216 226 235 204 571 199 225 281 208 308 575 285 351 306 546 322 366 531 308 318 670 704 75 748 691 732 642 638 537 1 248.61 MM 1 198.55 bb 1 474.76 MM 1 604.74 MM 1 548.31 bb 1 509.24 bb 1 416.56 bb 1 473.22 bb 1 482.12 MM 1 480.59 bb 1 532.80 bb 1 278.17 MM 1 451.67 bb 1 300.56 bb 1 336.95 bb 1 441.04 bb 1 480.18 bb 1 235.15 bb 1 343.87 bb 1 428.10 bb 1 414.05 MM 1* 287.31 bb 1 396.73 bb 1 225.86 MM 1 337.66 MM 1 331.42 MM 1 258.72 bb 1 466.87 bb 1 273.25 MM 1 482.50 bb 1 420.31 bb 1 431.38 bb 1 367.61 bb 1 398.50 bb 1 249.01 bb 1 355.24 bb 1 418.91 MM 1 314.98 bb 1 252.29 MM 1 387.24 MM 1 242.98 MH 1' 381.17 bb 1 366.66 MM 1 352.10 MM 1 164.71 MH 1 367.80 bb 1 168.62 bb 1 154.58 bb 1 161.28 MM 1 167.67 bb 1 146.02 bb \ 407.90 bb 1 142.43 bb 1 160.54 bb 1 200.47 MM 1 148.91 bb 1 220.14 bb 1 410.85 bb 1 203.31 bb 1 250.92 MM 1 218.67 bb 1 389.67 bb 1 229.80 bb 1 261.34 bb 1 379.16 bb 1 219.86 MM 1 226.93 MM 1 478.83 MM 1 502.64 bb 1 540.16 bb 1 533.98 bb 1 493.58 bb 1 523.07 MM 1 458.22 bb 1 455.91 bb 1 383.25 HM -50 -60 -4 22 11 3 -1 -4 -2 -3 8 -44 -9 -39 -32 -11 -3 -52 -30 .-13 -16 -42 -20 -54 -32 -33 -48 -5 -45 -2 -15 -13 -26 -19 -50 -28 -15 -36 -49 -22 -51 -23 -2 -29 -67 -2 -66 -69 -67 -66 -70 -17 -71 - -59 -70 -55 -17 -59 -49 -56 -21 -53 -47 -23 -55 -54 -3 2 3 8 0 6 -7 -8 -22 9ago Quantify Calibration taport , MX-130 Mat blaar l ! Calibration C:\MXaiUna\FACi-l.WUJ\Curruia\033499a ] Laat nodlfiad: Wad Mar 10 09:42:03 1*99 rrlntad! Mad Mar 10 09:44:01 199 BACK TO MAIN Quantify Calibration lteport TGX-120 Hat U w Calibration! C:\KXJSUnz\7iCT~l.no\CurraOS\032499a Laat nodlfiedi Wad Mar 10 09 z42: OS 1999 Prlntadf W ad War 10 09:44:01 1999 Compound 2 name: PFHS Correlation coefficient: r = 0.989537, rA2 = 0.979184 . . Calibration curve: 6.73075 x + 151.787' Response type: External Std, Area Curve type: Linear, Origin: Exclude, Weighting: Null, Axis trans: None 8.65e3n BACK TO MAIN *9 2 Soup 020199: Quantitated on 03/09/99 and printed on 03/10/99 by D M Quantiiy Calibratioa Jlaport xax-120 lut Livar Calibrai ioni Lue aodifladi yrlntadl C>MOM ad Mar Mad Mar U110M0SX9S\i:rM44Cii O0T1S-l1.1n91o991\Camn\0224S*m BACK TO MAIN oup 02019! Quantltatad on 03/09/99 and prlntad ea 01/10/99 fcytu Quantify Calibration Itaport vex-130 Hat Livar Calibration* C:\MMILYKX\rACT-l.ntO\Currain\022499a Last nodi Si a d t W a d Mar 10 09*43:05 1999 Print ad: Ma d Mar 10 09*44*01 1999 BACK TO MAIN Boup 020199* Quaatitatad on 03/09/99 and printed on 03/10/99 by SUB g u u t l t y Calibration Rapoct TOX-12Q Hat U n r Cal i b r a t i m i Ci\MUautn\rA-l .MO\Cu x v a \ 0 2 2 4 1 1 a bast aodiiiadt ad Mar 10 01:12:00 1111 rrlacadi Wad Mar 10 02:44:01 1212 BACK TO MAIN loop 0201221 Qoantitatad oa 03/02/22 aad printad on 03/10/21 b y M Quantify Calibratioa Raport S0K-130 Rat Livar ' C a lib ra tim i C:\XUILniX\riCT-l.rao\CuirTaDa\0224fla U s e aedlfiadi a d Mar IO 09)43)05 1999 yrintadl Msd Mar 10 09)44)01 199 ` ' . BACK TO MAIN rag | ' ' 1 ' . oup 030199) Quantltatad on 03/09/99 and printad on 03/10/99 by m Method Report Method files Last modified: Compounds Printed: Cl\KASSLTOX\FACT-l.FRO\H.thDB\022i99.ndb Fri Tmb 2< 10i3 0 i39 1)9* ' Is PFOS Tue Mar 09 13s22s01 1999 . Compound Kama: Internal Ref.> Quantify Tracei Cone, of Standardsi User W Value t User Peak Faeton Integrate Window Faeton PFOS INone] TIC Cone. A 1.000000, Disabled 1.000000 2.0 0 0 -- Soup 0201^ Retention Tine (sins) i Relative Retention Tines Tine W indow (mins) t Peak Selections 1.030, 0.000, Disabled 1.030 Response Types Responses Internal Areas * Polynomial Types Point of Origins Fit Weightings Axis Transformations Linear exclude Hone Hone Unites Peak-to-peak amplitudes 185 Smoothings Window size (scans)s Number of ssnooths: Smoothing methods Disabled 1 2 Join valley thresholds Reduce peak tailings Raise baseline maxiaums 30.00 70.00 4.00 Draw vertical thresholds Deteet shoulderss Shoulder thresholds . 10.00 Disabled 10.00 Relative heights Absolute heights Relative areas. Absolute areas 50.00, 0.00, Disabled 3.00, Enabled .0.00, Disabled * LAc 0 3 /09/99 BACK TO MAIN Page 1  Method Report | Method files Last modified! 1 Compounds Printed! CsVMASSLYMX\FACT-l.PRO\MethDB\022499a.mdb . Fri Feb 2( 10s30s39 1999 2s PFHS Tue M ar 09 13s22s01 1999 I Compound Hasses | Internal Raf.s Quantify Tracas Cone, of Standards! Usar W Valas Usar Paale Factors Integrate Window Factors FFHS {Hone] TIC Cone. A . 1.000000, Diaablad 1.000000 2 .0 0 0 Retention Time (mins) s Relativa Ratention Tinas 1 Tina Window (nina) s Peak Selectiont .563, Knablad 0.000, Diaablad 1.117 Haarest ' * ] Response Types Responses Polynomial types ] Point of Origins Fit Weightings Axis Transformations Rxternal Areas Linear Kxclude Hone Hone Unitss 1 ' Peak-to-peak amplitudes ! 18S 1 Smoothings Window .is. Ii c i d i ) i Humber of smoothes Smoothing methods j . Disabled 1 2 1 Join valley thresholds * Reduce peak tailings Raise baseline maximum! Y 30.00 70.00 4.00 1 Draw vartical thresholdt Detect shoulderss Shoulder thresholds .* 10.00 Disabled 10.00 ] Relative heights Absolute heights Relative araat Absolute areas 1 50.00, Rnabled , 0.00, Disabled .00, t u b l a d 0.00, Disabled 3 BACK TO MAIN lag. 2 5nu0F A2DI44 _LAC Qiloilw J J I i BACK TO MAIN Method Report Method filet Last modified! Compound! Printed t C:\KASSLYNX\FACT-l.FRO\MethDB\022499a.mdb . Fri Feb 26 10t30t39 1999 3f POAA Tue Mar 09 13i22i01 1999 . Compound Hemet Internal Ref.i Quantify Traai. Cone, of Standards Usar KT Valuat Usar Peak Faeton . Integrata Window Faeton POAA [Hone] TZC Cone. A . . 1.000000, Disabled 1.000000 2 .0 0 0 Retention Time (nine) i Relative Retention Timet Time Window (mins) t Peak Selection! 7.458, Xnabled 0.000, Disabled 0.902 Nearest ' Response Typet Response! Internal Areas * , * Polynomial Type* Point of Origini Fit Weighting! Axis TransformationI t.in e a r Exclude 3(one Hone Unitsi Peek-to-peek amplitudet 185 . . ' Smoothing! Window sise (scans)t Number of smooths: Smoothing method! Join valley threshold!' Reduce peak tailing! Raise baseline maximum: . Disabled 1 2 30.00 70.00 4.00 Draw vertical threshold! Detect shoulders! Shoulder threshold! .* 10.00 Disabled 10.00 ' Relative height: Absolute height! Ralativ. u n i ' Absolute areat ' 50.00, Enabled , 0.00, Disabled 3.00, f e a b l d 0.00, Disabled ' ' Soup nloiM __ M C o:lionlw r ' t*v ps . ] * , Method Report Method file: Last modified: Compound: Printed: Cl \MASSLYMX\ FACT-1. PRO\M.tM)B\022499A.Mdb . Fri Fall 2( 10(30.39 1999 4l MS 13 Tue Mar 09 13:22:01 1999 . Compound Ni h i Internal Ref.: Quantify Trac: Cone, of Standarda: Var R7 Vaiti i Uar Peak rector: Integrate Window Factor: MS 13 (Ko m ) TIC Cone* A . 1.000000, Disabled 1.000000 2.000 Retention Tin (mina): 1.648, enabled Relativo Retention Timex 0.000, Disabled 1 Time Window (mine) : 0.730 Peak Selection! . Nearest . Response type: Response: Internal Areas Polynomial type: 1 Point of Origin: J Pit Weighting: I Axis Transformation': Linear Exclude None Nne Units: 1 Peak-to-peak amplitude: 185 Smoothing: Window aixa (scans): Number of smooths: Smoothing method: Disabled '. 1 2 Join valley threshold: Reduce peak tailing: Raise baseline maximum: 30.00 70.00 4.00 Draw vertical threshold: Detect shoulders: Shoulder threshold: . 10.00 Disabled 10.00 J Relative height: . Absolute height: Relative area: Absolute area: 50.00, Enabled 0.00, Disabled 3.00, Xoabled 0.00, Disabled ~Souo A 2 o m BACK TO MAIN * LAC Olttfa_______ *S. 4 BACK TO MAIN MuChad Report Mltfcod filBi Laic aodifildi Compoundi Frincidi .Cl \KASSLYHX\FACT-l.PKOVK*thDB\022199l'BKlb Fri Fib 26 X0t30i]9 1999 5t K613 Till Mur 09 U i 2 2 > 0 1 1999 *Compound Maati Internal Ref. Quantify Tracii Cone, of Standardst - User RF Valuet User Peak factor* Integrate Window Factor* . MIS [None] TIC Cone. A . '1.000000, D i n b l i d 1.000000 2.000 *Retention Time (nine) Relative Retention Time* Tine Window (mine) t Peak Selection* . 9.S91, Xnablld 0.000 Disabled 0.710 Nearest - Response Type* Response* Polynomial Type* Point of Origin* Pit Weighting* Axil Traaifomationi *I x t i n u l Areas * . * t.lear Uapcmclude None - - * Sduo 020ltf 1 units* Paak-to~peak amplitude* 185 * *' *Smoothing* Window sire (scans) Number of smooths* Smoothing method* 'Join valley threshold** Reduce peak tailing* Raise baseline maxims** D1isabled 2 30.00 70.00 4.00 Draw vertical threshold* Dstect shoulders: Shoulder threshold* 10.00 Disabled 10.00 * . Relative Absolute hheeiigghhtt** Relative area*. Absolute area* , 50.00, Enabled 0.00, Disabled 3.00, Enabled 0.00, Disabled * L A C 0 3 lefih $ _ i *. 1 Method Report Method file: , C:\MASSLrMX\PACT-l.PRO\MethDB\022499a.mdb Last modified: Fri Feb 26 10:30:39 1999 Compound: 6: ZS Printed: Tue Mar 09 13:22:01 1999 Ceopound taui Internal Raf.t Q uantify Traai Cone, of Standardai Q aer XT V alu ti Uaar Faak F aeton I n te g r a ta Window F a e to n is [None] TIC Cone, a . 1.000000, Diaablad 1.000000 2 .0 0 0 Retention Tina (mina) i 7.410, b a b i e d Relative Retention Tine: 0.000, Disabled ] Tine Window (nine) i Peak Selection: 0.980 Nearest Response Type: external Areas - # Polynomial Type: Point of Origin: ! Pit Weighting: Axis Transformation: Units: Peak-to-peak amplitude: I Smoothing: Window sire (scans}: Number of smooths: Smoothing method: Linear exclude None None 185 Disabled 1 2 Join valley threshold: Reduce peak tailing:' Raise baseline maximum: 30.00 70.00 4.00 Drew vertical threshold: . Detect shoulders: Shoulder threshold: 10.00 Disabled 10.00 J Relative height: Abaolu t a haighti Relative area: Absolute area: 50.00, Enabled 0.00, Diaablad 3.00, b a b i e d 0.00. Disabled BACK TO MAIN -- n io mS dud r *- U c lo^l Fag. i BACK TO MAIN :& b EM*M ADELINE 041098: FA CT E quipm ent Log 98053 Q uattro II, #6443E PAGE DATE: 03/01/99 ANALYST: drb '1 . STUDY NUMBER T O X 120 MATRIX: rat liver DESCRIPTION: liver cxtr. dilutions FACT M ethod: Injection vn1nmff,lQ uL Cycle tim e: 16 m in. Electrode: Z-Spray M obile Phase: (A)4.0 mM N H 4CH 3C02 . (B) MeOH_____ TN-A- or stndrd num ber (A) 99011-1-9__________ (B) TN-A-2384 Prepared (date/by): E x p ires: ` (A) 2/12/99 m ee (A) 08/12/99___________ (B) 2/20/99 drb (B) 8/18/99 Store at room tem p? [Yj o r N . O ther._______________________ G u ard colum n: None____________ . . Part num ber na _______ Lot num ber na_______________________ A nalytical colu m n ; keystone betasil__________ __ ______________________________ Size: 2 x 100mm__________. Type: c l 8 __________ Sphere size: Sum P art num be r 105-701-2______ L ot numbe r p8f!3 Serial number 118739n A nalytical p aram eters: ' D rying gas (L/hr): 300 ESI nebulizing gas CL/hr) 15 LC Flow rate: 300 uL/min. B ackpressure: 120 bar Sheath gas (L/hr)_0 . H PL C solvent g rad ien t: long2ex Tim e (m in.) 1) 0.00 2) 8.S 3 1.1.0 & 11.5 5) 12.5 %B 40 90 90 100 100 . BACK TO MAIN Tuning Method Report ' Method: C :\MASSLYNX\RATS. PRQ\ACQUDB\PFOS 0$ # Printed: M o n M a r 01 17:17:40 1999 MacUliru. CHloW fa LMsCx/lolil Page 1 ] ] 1 MS SOURCE ( ESP- ) Capillary Cone Extractor J RF Lens Source Block Temp. Desolvation Temp. I 1 Pressures * Analyser Vacuum I Gas Cell I Set Rdbk 3.00 45 5 0.2 215500 3.00 -46 -4 215513 MSI LM Resolution HM Resolution ' Ion Energy Ion Energy Ramp MLuelnstip6lier Rdbk 6.4e-5 5.3e-3 .*X MS2 . LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier Set Rdbk 15.0 15.0 2.0 ? 0.0 ' 55 650 Set Rdbk 15.0 15.0 20 ' 20 2.0 0 .0 40 0 650 41 18 I I Scanning Hathed Kaport Hathod: C:\HMSUftOC\FATS.FJO\ACQUD8\TOX120A Last Modified: Hon Mar 01 17:16:31 1999 Printadl Man Mar 01 17:17:51 199 Solvant Delay | mina ) I 0.00 runotion : 1 HIM of 3 M a x Pairs ( ESP- ) ' Inter Channal Daisy ( Sacs ) t Span ( Daltons ) t Start Tins ( Mins ) : End Tima t Mins J : Repeats t Chinns! Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell (Sacs) Coll Energy (aVJ 1 399.00 80.00 0.25 45 2 399.00 99.00 0.25 45 3 399.00 130.00 0.25 45 - Cone 60 60 60 Function 2 2 M M of 3 M u s Pairs ISP- ) Inter Channel Delay ( Sacs ) : Span ( Daltona ) : Start Tima ( Mins ) i End Tima ( Mins ) i Repeats l Channel Parent Daughtar 0.03 0.00 0.00 13.50 1 Dwell (Sica) Coll En.rgy (aV) 1 499.00 0.00 0.25 2 499.00 99.00 0.25 3 499.00 130.00 0.25 45 45 45 Cone 60 60 60 Function ! 3 MFM of 2 Maaa Pairs t . Inter Channel Delay ( Sacs ) i 0.03 Span ( Daltona ) i ~ 0.00 Start Tima ( Mins ) i * 0.00 End Tima ( Mins ) : 13.50 Repeats : . 1 Channel Parant Daughtar Dwell lS.cs) Coll Energy (.V) 1 299.00 80.00 . 0.25 2 299.00 99.00 0.25 45 45 Cone 35 35 Function : 4 HIM of 3 Maas Pairs ( ESP- J Inter Channel Delay ( Sacs ) i Span { Daltons ) t Start Tima ( Mins ) s. . End Tima ( Mins ) : Repeats t Channel Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell (Secs) Coll Energy (eV) Cone ( V ) 413.00 119.00 413.00 169.00 413.00 219.00 0.25 0.25 0.25 30 20 30 15 15 15 Function t 5 MEM of 3 Mass Pairs ( ESP- ) Inter Channel Delay ( Sees ) 2 Span ( Daltons j : Start Time ( Mins ) t ' End Time ( Mina ) 2 Kep.ats i Channal Parent Daughtar 0.03 0.00 0.00 13.50 1 Dw.ll (Secs) Coil Energy (eV) Cone ( V ) 1 469.00 169.00 0:25 2 469.00 219.00 0.25 3 469.00 119.00 0.25 45 * 45 45 30 30 30 Fonation i S M M of 3 Maas Pairs ( ESP- ) Intar Channel Delay ( Sacs ) * Span ( Daltona ) t Start Tima ( Mins ) s End Tima ( Mina ) t Repeats t * Channel Parent Daughter 0.03 0.00 0.00 13.50 i Dw.ll (Secs) Coll Energy (eV) Cone (( V ) 1 569.00 169.00 0.25 2 569.00 219.00 0.25 3 569.00 119.00 0.25 45 45 45 35 35 35 function : ? KMC of 1 Mass Pair ( ESP- ) Zntsr Channel Delay ( Secs ) t 0.03 Span ( Daltons ) t 0.00 Start Time ( Mins ) * 0.00 End Time ( Hina ) t 13.50 Repeats I ' ,,1 Channel Parent Daughter Dwell (Sacs) Coll Energy (aV) Cone ( V )1 1 427.00 80.00 0.25 4Q 60 & u c <*/iotn BACK TO MAIN ' Pag* 1 M asslynx - Sam ple List Sample List: C:\MASSLYNX\rats.PRO\SampleDB\030199c.SPL Printed: Mon Mar 0117:17:14 1999 BACK TO MAIN Page 1 I File Name File Text . MS File In le t File B ottle F acto r 1 Sample 1 030199001 MeOH Blank TOX120A long2 91 0 Analyte 2 030199002 MeOH Blank TOX120A long2 91 . 0 Analyte 3 030199003 Tox 120. RBL02229-H2O B lk -1 ,2/22/99 4 030199004 Tox 120, RBL02229-RBUverBlk-1,2/22/99 TOX120A TOX120A long2 long2 .1 2 0 Analyte 0 Analyte 5 030199005 Tox 120, RBL02229-5ppb-1.2/22/99 6 030199006 Tox 120, RBL02229-10ppb-1,2/22/99 TOX120A TOX120A long2 long2 3 4 0 Standan . 0 Standan ] 7 030199007 Tox 120, RBL02229-25ppb-1,2/22/99 8 030199008 Tox 120, RBL02229-50ppb-1,2/22/99 TOX120A TOX120A long2 long2 5 6. 0 Standan 0 Standan 9 030199009 Tox 120, RBL02229-1OOppb-1,2 /2 2 /9 9 1 TOX120A long2 7 0 Standan I 1 0 030199010 Tox 120, RBL02229-250ppb-1,2/22/99 1 1 030199011 Tox 120, RBL02229*500ppb-1,2 /2 2 /9 9 1 2 030199012 Tox 120, RBL02229-750ppb-1,2/22/99 TOX120A TOX120A . TOX120A long2 long2 long2 8 9 10 ' 0 Standan 0 Standan 0 Standan 13 030199013 Tox 120, RBL02229-1000ppb-1,2 /2 2 /9 9 TOX120A long2 . 1 1 0 Standan 14 030199014 MeOH Blank . TOX120A . long2 91 0 Analyte 15 030199015 ' Tox 120 DNO. 125F-G 2,2/22/99 D5 1 16 030199016 Tox 120 DNO. 126F-G2, 2/22/99 D5 17 030199017 Tox 120 DNO. 127F-G2, 2/22/99 D5 TOX120A TOX120A TOX120A long2 long2 long2 . 12 13 14 0 Analyte 0 Analyte 0 Analyte 18 030199018 Tox 120 DNO. 129F-G 3,2/22/99 D2 TOX120A long2 ' 15 0 Analyte 19 030199019 Tox 120 DNO. 130F-G3, 2/22/99 D2 I 2 0 030199020 Tox 120, RBL02229-250ppb-CC1,2 /2 2 /9 9 2 1 030199021 MeOH Blank TOX120A TOX120A TOX120A long2 ' long2 lo n g 2. 16 17 92 0 Analyte 0 Analyte 0 Analyte 2 2 030199022 Tox 120 DNO. 131F-G 3,2/22/99 D2 TOX120A long2 18 0 Analyte 23 030199023 Tox 120 DNO. 132F-G 3,2/22/99 D2 TOX120A long2 19 0 Analyte ] 24 030199024 Tox 120 DNO. 133F-G3, 2/22/99 D2 25 030199025 Tox 120 DNO. 134F-G 3,2/22/99 D2 TOX120A TOX120A long2 long2 20 21 0 Analyte 0 Analyte 26 030199026 Tox 120 DNO. 135F-G3, 2/22/99 D2 TOX120A long2 22 0 Analyte 27 030199027 Tox 120, RBL02229-250ppb-CC2,2/22/99 TOX120A long2 23 0 Analyte 28 030199028 MeOH Blank ' TOX120A long2 92 - 0 Analyte 29 030199029 Tox 120 DNO. 136F-G 3,2/22/99 D2 TOX120A long2 24 0 Analyte 30 030199030 Tox 120 DNO. 137F-G 3,2/22/99 D2 TOX120A long2 25 0 Analyte 31 030199031 Tox 120 DNO. 1 38F -G 3,2/22/99 D2 TOX120A long2 26 0 Analyte 32 030199032 Tox 120 DNO. 139F-G 3.2/22/99 D2 TOX120A long2 27 0 Analyte i 33 030199033 Tox 120 DNO. 141F-G 3,2/22/99 D2 34 030199034 Tox 120, RBL02229-250ppb-CC3,2/22/99 TOX120A TOX120A ' long2 long2 28 29 0 Analyte 0 QC 35 030199035 MeOH Blank TOX120A long2 93* 0 Analyte J 38 030199036 Tox 120 DNO. 142F-G 3,2/22/99 D2 37 030199037 Tox 120 DNO. 143F-G 3,2/22/99 D2 38 030199038 Tox 120, RBL02229-250ppb-CC4, 2/22/99 39 030199039- MeOH Blank TOX120A TOX120A TOX120A TOX120A long2 long2 k>ng2 long2 30 ; 31 32 93 0 Analyte 0 Analyte 0 QC O Analyte 40 030199040 Tox 1 2 0 ,106F-G1-250ppb-MS, 2/22/99 TOX120A long2 33 0 Analyte 41 030199041 Tox 1 2 0 ,1 06F-G1-250ppb-MSD, 2/22/99 TOX120A long2 34 0 Analyte 1 42 030199042 Tox 120, RBL02229-250ppb-CC5,2/22/99 TOX120A long2 35 0 QC 43 030199043 MeOH Blank TO X120A .-V long2 93 . 0 Analyte 44 030199044 Tox 120, RBL02229-H2O B lk -2 ,2/22/99 J 45 030199045 .Tox 120, RBL02229-RBLiverBlk-1,2/22/99 46 030199046 Tox 120, RBL02229-5ppb-2,2/22/99' 47 030199047 Tox 120, RBL02229-1Oppb-2,2/22/99 48 030199048 Tox 120, RBL02229-25ppb-2,2/22/99 TOX120A TOX120A TOX120A TOX120A TOX120A long2 long2 long2 long2 long2 . ' 36 37 38 39 40 0 Analyte 0 Analyte 0 Standai 0 Standai 0 Standai 49 030199049 Tox 120, RBL02229-50ppb-2,2/22/99 J 50 030199050 Tox 120, RBL02229-100ppb-2,2/22/99 51 030199051 Tox 120, RBL02229-250ppb-2,2/22/99 TOX120A TOX120A TOX120A long2 long2 long2 41 . 42 43 0 Standai 0 Standai 0 Standa 52 030199052 Tox 120, RBL02229-500ppb-2,2/22/99 53 030199053 Tox 120, RBL02229-750ppb-2,2/22/99 TOX120A TOX120A long2 long2 44 45 0 Standa 0 Standa I 54 030199054 Tox 120, RBL02229-1000ppb-2,2/22/99 TOX120A long2 46 0 Standa j V\ iVVaklW 03/*/ OV/oy 1 FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN Date: 0 3 / o s M Analyst: FACT Study Number ~Tb'C 1 ^ 0 Matrix Time Points Xixsf&' 13 Dilutions? J Analyte 1: V D S>1V Acceptable lin ear Range: Q fb LOQ for this data s e t Re-evaluated fa: different range? A^o Analyte 4: Acceptable Linear Range: / '^ 1 QDO LOQ for this data set: / Q ______ Re-evaluated for different range? f\ ) O Anafyte5: Acceptable linear Range: / OQ3ppk> LOQ fix this data set: 7 ^ 0 Re-evaluated fix different range? $> Teio*w cLk' V Analyte 2: B3S Acceptable Linear Range: / Q ** )j LOQ far this data se t J _______ Re-evaluated fix different range? fJ o J Analyte 3: Acceptable Linear Range: &c&ppb 3 LOQ fix this data set: Re-evaluated for different range? t Analyte 6: ~ S ie * / Acceptable LOQ for this data set Re-evaluated for different range? a / d A nalyte 7: Acceptable Linear Range: LOQ for this data set _____ Re-evaluated for different range? BACK TO MAIN Quantify Ccapound Suanary Kaipert TOX 120 DHO Rat Liras Group. i t ] Saapl. List: M:VRAI3.PBO\3uplMa\030199a Last nodifiad: Mon Mas 08 08:49:2 1*99 Mathod: M:\rats.HW\MathEB\010199a Last nodifiad: Mon Mas 08 10:03:92 1999 Job Coda: P rin ta d : Mon Mas 08 10:35:49 1999 w >. cjltttf Paga 1 Cooperad 1 : FFOS Kano 1 030199001 S o c ia lis t Taxt MaOK Blank ' * l t d Cono RI A nalyt 7.929 Araia D H .T act. Cono fla g %Dv 12509 1 286.71 MK 2 030199002 HsOH Blank A n aly t 7.913 1971 1 4.03 MK 3 030199003 Toa 120, RBL02229-H2O B lk-1, 2/22/9 A n aly t 7.959 1609 1 0.00 MK 4 030199004 Tox 120, BBL0222-RBUvarBlk-l, 2/22/99 A n aly t 7.964 22(5 1 13.96 MK 5 030199005 Tox 120, BBL02229-Sppb-1, 2/22/99 Standa 5.99 8.073 3267 1 ' 41.14 bbX 587 6 030199006 Tox 120, ABL02229-10ppb-l, 2/22/99 Stands 12.00 7.899 2555 1 22.20 fab 85 7 030199007 Tox 120, RBL0 2229-25ppb-1 , 2 /22/99 Standa 29.90 7.927 2937 1 32.36 bb 8 8 030199008 Tox 120, RBL02229-50ppb-l, 2/22/9 Standa 59.90 8007 (107 1 . 63.44 Hf 6 9 030199009 Tox 120, BBL02229-100ppb-l, 2/22/9 Standa 120.00 8.023 6367 1 123.50 MK 3 10 030199010 Tox 120, BBL02229-250ppb-l, 2/22/99 S tanda 299.00 8.002 12968 1 299.44 MK 0 3 11 030199011 12 030199012 13 030199013 Tox 120, RBL02229-50Oppb-1, 2/22/99 Tox 120, RBL02229-75Oppb-1, 2/22/99 Tox 120, RBL02229-1000ppb-l, 2/22/9 Standa Standa Standa 599.00 898.00 1198.00 8.043 8.017 -8.000 2SS(( 1 37384 1 (5(78 1 633.05 bb 947.68 bb 1162.75 bb 6 6 -3 14 030199014 MaOH B lank A n aly t 7.871 1341 1 0.00 bb 15 030199015 Tax 120 DHO. 125T-G2, 2/22/99 DS A n aly t 7.858 1796 1. 2.05 bb 18 030199016 Tox 120 DHO. 126F-G2, 2/22/9 DS . Bnalyt 7.875 1820 1 2.69 bb 17 030199017 Tox 120 DHO. 1277-C2, 2/22/9 DS A n aly t 7.857 16(5 1 0.00 bb 18 030199018 Tox 120 DHO. 1297-G3, 2/22/99 D2 A nalyt 7.895 228 1 15.14 bb 19 030199019 Tex 220 DHO. 1307-4B, 2/22/99 D2 A nalyt 7 879 2367 1 17.21 MK 20 030199020 Tox 120, RBL02229-250ppb-CCl, 2/22/99 QC 299.00 8.051 11524 1 ,260.53 bb -13 21 030199021 MaOK Blank A nalyt 7.896 1(S 1 0.00 bb 22 030199022 Tox 120 DHO. 1317-03, 2/22/99 D2 A n aly t 7.819 2(98 1 20.70 MK T 23 030199023 24 030199024 25 030199025 Tox 120 DHO. 1327-03, 2/22/99 D2 Tox 120 DHO. 1337-63, 2/22/99 D2 Tox 120 DHO. 13*7-63, 2/22/9 D2 A n aly t A n aly t A nalyt 7.827 7.901* 7.822 2(70 1 2356 1 2315 1 19.95 MK 16.92 MK 15.83 MK 26 030199026 .T o x 120 DHO. 1357-63 , 2/22/99 D2 A n aly t 7.833 2363 1 17.12 MK 27 030199027 Tox 120, HBL02229-250ppb-CC2, 2/22/99 oc 299.00 8.005 10(71 1 232.56 MK -22 28 030199026 MaOK B lank A n aly t 7.919 ' 14(3 1 0.00 bb 29 030199029 Tox 120 DHO.. 1367-63, 2/22/99 D2 A n aly t 7.887 2196 1 . 12.67 MK 30 030199030 Tox 120 DHO. 1377-63, 2/22/99 D2 A nalyt 7.869 1888 1 4.49 MK 31 030199031 Tox 120 DHO. 1387-63, 2/22/99 D2 A nalyt 7.854 1834 1 3.07 KM 32 030199032 Tox 120 DHO. 1397-63 , 2/22/99 D2 A n aly t 8.020 2358 1 16.99 bb 33 030199033 Tox 120 DHO. 1417-63, 2/22/99 D2 A nalyt 7.869 2612 1 23.74 bb 34 030199034 Tox 120, RBL02229-250ppb-CC3, 2/22/99 QC 299.00 8.017 11(01 1 257.26 bb -14 35 030199035 MaOH Blank A nalyt 7.933 1302 1 0.00 bb 36 030199036 Tox 120 DHO. 1(27-63, 2/22/99 D2 A n aly t 7.854 2228 1 13.53 MK 37 030199037 Tox'120 DHO. 1(37-63, 2/22/99 D2 A nalyt 7.929 2526 1 21.44 bb 38 030199038 Tox 120, RBL02229-250ppb-CC(, 2/22/99 . QC 299.00 8.023 11556 1 261.39 bb -13 39 030199039 Mam B lank A n aly t 7.932 1265 1 0.00 MK 40 030199040 Tox 120, 1067-Gl-250ppb-KS, 2/22/99 A n aly t 8.034 2(68 1 610.36 KM 1 41 030199041 42 030199042 Tox 120, 1067-61-250ppb-MSD, 2/22/9 Tox 120, BBL02229-2S0ppb-OCS, 2/22/9 43 030199043 MaOH B lank A n aly t QC A n aly t 299.00 8.015 8.043 7.864 25982 1 11767 1 1(0 1 644.70 MK 266.98 bb 0.00 KM -11 44 030199044 Tox 120, BBL02229-H20 B lk-2, 2/22/9 ' A nalyt 7.885 168 1 0.00 bb 45 030199045 Tox 120, BBL02229r BBLivarBlk-l, 2/22/99 A n aly t 7.898 13(1 1 0.00 MK . i 46 030199046 47 030199047 48 030199048 Tox 120, BBL02229-5ppb-2, 2/22/9 Tox 120, RBL02229-10ppb-2, 2/22/99 Tox 120, HBL02229--25ppb-2, 2/22/99 Standa Standa .Standa 5.99 7.915 12.00' 7.845 29.90 7.995 1884 1 1952 1 2830 1 1.39 bb 6.18 MK 29.51 MM -27 -49 -1 49 030199049 Tox 120, RBL02229-50ppb-2, 2/22/9 Standa 59.90 8.011 3802 1 25.34 bb -a SO 030199056 51 030199051 Tax 120, BBL02229-100ppb-2, 2/22/9 Tox 120, BBL02229-2S0ppb-2, 2/22/9 Standa Standa 120.00 7.988 299.00 8.002 6276 1 1277 1 121.15 bb 293.88 bb 1 -2 52 030199052 Tox 120, 9BL02229-S00ppb-2, 2/22/99 Standa 599.00 7.997 23131 1 568.95 MM -5 S3 030199053 Tox 120, 8BL02229--750ppb-2, 2/22/9 Standa 898.00 8.045 32672 1 822.46 bb -8 54 030199054 Tox 120, BBL0222-1000ppb-2, 2/22/99 Standa 1198.00 8.031 ..(x8811 1 1251.30 bb 4 J *adollne041098:: quantitated and printad 03/08/99 by deb I nad*llna041098:: quantitated and prlntad 03/08/99 by deb 8 8 8 ? ? ? 8 8 ? f P 8 ^ifi S i ^ ^ g 88 g p f 8 8 8 f i P ^ 8 8 s f ^8 s s f Sfili! SSSSSBSSSSS i fl!S g 15i s ar i l i H | P P r t P P P i P P p p11| *i1P1V P1 vri 8ii; ; ; ; ; ; ; ; ; ' 8 a a ! ;$s I ss1 m B S b S S S b s b s b S b b b b b S ^ s s s k SSSSSSST, !S5 -- Si $ 58 2 S 5 S ' S S S S """"' ; IFI 1 IVi lSi{ I il a n a l J S 383 35 K h I o ? la S Bg " 8 0 M/8'H?B w" " 1 *f*f 1nMe O'NW MH (CM H iaoN( K> M ARAftAAAAAA U^ tAUAUAKUI* AA AUUU<HAOA AAA touO(s*--enJ -iJn H A O liiillsHss 111 I 00 O U M isSsSKoo SSo 5 . s s s s s s s s i s oui: s 3 5 3 3 3 3 3 3 ??* ? ? 3 ? H oii.K.iC.LSS I S I S g s s 3 3? mm rm* HWaWanIAOlN UoHKtAMNKA AAAAAAAAAA A AAAAAAAAA isissss ? ,3S S S S 5 K S 8 sssssbssss 33333333g? CD > O 7 ? BACK TO MAIN Quantify Compound S m e a r y Raport TOX 120 W O Rat U m Groups 2 ft 3 Saapla List: M:\RAM.PR0\SasplsBH\030199o Last aodifisd: Mon Mar 08 09:49:28 1999 Mathod: M:\rats.PRO\MathU8\030199a Last aodifiad: Mon Mar 08 10:03:52 1999 Job Coda: Printed: Mon Mar 01 10:35:4 IS Compound 3: Mans 1 030199001 2 030199002 3 030199003 4 030199004 5 030199005 6 030199006 7 030199007 8 030199008 9 030199009 10 030199010 11 030199011 12 030199012 13 030199013 14 030199014 IS 030199015 18 030199016 17 030199017 18 030199018 19 030199019 20 030199020 21 030199021 22 030199022 23 030199023 24 030199024 25 030199025 26 030199026 27 030199027 28 030199028 29 030199029 30 030199030 31 030199031 32 030199032 33 030199033 34 030199034 35 030199035 36 030199036 37 030199037 38 030199038 39 030199039 40 030199040 41 030199041 42 030199042 43 030199043 44 030199044 45 030199045 46 030199046 47 030199047 48 030199048 49 030199049 50 030199050 51 030199051 52 030199052 53 030199053 54 030199054 aaplaliat last , MaOH Blank MaOH Blank Ion 120, BBL02229-H2O Blk-1, 2/22/99 Ton 120, KBL02229-KBX*ivarBlk-l, 2/22/99 Ton 120, *BL0222-5ppb-l, 2/22/99 Ton 120, RBL02229-10ppb-l, 2/22/99 Ton 120, KBL02229-25ppb-l, 2/22/99 Ton 120, RBL02229-50ppb-l, 2/22/99 Ton 120, SBL02229-100ppb-l, 2/22/99 Ton 120, RBLO2229-250ppb-l, 2/22/99 Ton 120, BBL02229-500ppb-l, 2/22/99 Ton 120, 8BL02229-750ppb-l, 2/22/99 .Ton 120, NBL02229-1000ppb-l, 2/22/99 MaOH Blank ' Ton 120 DMO. 125F-432, 2/22/99 DS Ton 120 DMO. 126F-62, 2/22/99 D5 Ton 120 DMO. 127F-G2, 2/22/99 D5 Ton 120 DMO. 129F-G3, 2/22/99 D2 Ton 120 DMO. 130F-G3, 2/22/99 D2 Ton 120, KBL02229-250ppb-CCl, 2/22/99 MaOH Blank Ton 120 DMO. 131F-G3, 2/22/99 D2 Ton 120 DUO. 1321-03, 2/22/99 D2 Ton 120 DNO. 133F-G3, 2/22/99 D2 Ton 120 DMO. 134P-G3, 2/22/99 D2 .Ton 120 DNO. 13SF-03, 2/22/99 D2 Ton 120, MBL02229-250ppl>-CC2, 2/22/99 MaOH Blank - Ton 120 DNO. 136F-G3, 2/22/99 D2 Ton 120 DNO. 137F-G3, 2/22/99 D2 Ton 120 DNO. 138F-G3, 2/22/99 D2 Ton 120 DNO. 139F-G3, 2/22/99 D2 Ton 120 DNO. 141F-G3, 2/22/99 D2 Ton 120, RBL02229-250ppb-CC3, 2/22/99 MaOH Blank Ton 120 DNO. 142F-G1, 2/22/99 D2 Ton 20 DNO. 143F-G3, 2/22/99 D2 . Ton 120, BBL02229-2S0ppb-CC4, 2/22/99 MaOH Blank Ton 120, 106F-Gl-250ppb-MS, 2/22/99 Ton 120, 106F-61-250ppb-MSD, 2/22/99 Ton 120, BBL02229-25Oppb-CCS, 2/22/99 MaOH Blank Ton 120, BBL02229-H2O Blk-2, 2/22/99 Ton 120, RBL02229-NBUvarBlk-l, 2/22/99 Ton 120, BBL02229-5ppb-2, 2/22/99 Ton 120, RBL02229-10ppb-2, 2/22/99 Ton 120, KBL02229-25ppb-2, 2/22/9 Ton 120, BBU>2229-50ppb-2, 2/22/99 Ton 120, RBL02229-100ppb-2, 2/22/99 Ton 120, *BW2229-2S0ppb-2, 2/22/99 Tan 120, *BL02229-500ppb-2, 2/22/99 Ton 120, BBL02229-750ppb-2, 2/22/99 Ton 120, BBL02229-1000ppb-2, 2/22/99 ' aft n lctifft Papa 3 Std Cono Analyt Analyt Analyt Analyt Standa 5.99 Stands 12.00 Stands 29.90 Stands 59.90 Stands 120.00 Stands 299.00 Stands' 599.00 Stands 898.00 Stands 1198.00 Analyt Analyt Analyt Analyt Analyt Analyt QC 299.00 Analyt Analyt Analyt Analyt Analyt Analyt QC 299.00 Analyt Analyt Analyt Analyt Analyt Analyt QC 299.00 Analyt Analyt Analyt QC 299.00 Analyt Analyt Analyt QC 299.00 Analyt Analyt Analyt Stands 5.99 Stands Stand* 12.00 29.90 Stands 59.90 Stands 120.00 Stands 299.00 Stands 599.00 Stands 898.00 Stands 1198.00 KT Ai m Dll.Fast. Cano Flap. %DV 4.128 4.138 4.124 4.143 4.213 4.20 >7 1 212 1 399 1 659 1 1110 1 1381 1 48.06 bb 153.71 bb 323.86 bb 561.92 bb 974.76 bb 1222.81 bb -20 28 8 -6 9 2 4.147 401 1 4.171 473 1 325.85 bb 9 392.1 bb 31 4.03 4.046 4.390 4.297 4.057 389 1 389 1 377 1 258 1 427 1 4.247 4.247 4.15 4.163 4.145 4.129 . 102 1 176 1 388 1 601 1 1036 1 3,313 1 314.75 bb 5 314.84 bb 303.93 bb 195.04 bb 350.10 bb h 5 17 52.25 bb 120.05 bb 314.63 bb 508.68 bb 906.92 bb 1160.16 bb -13 0 5 -15 1 --3 a*deline04108:: quantitated ad printed 03/08/9 by drb V OI - * 1O HNNHAH0O O V> 4 O H * 7 7 7 H 1 *- 7 T <1 C 11.91 MH 26.17 MM 61.26 K M 106.51 M M 311.01 MK 591.1 bb 965.76 bb 1192.13 bb 77.90 bb 77.88 MK .287.96 bb 106.08 bb 99.25 bb 81.58 M K 72.71 bb 9.69 M M 250.93 MH ts 3 3 3S3 - m g5 D 0 flO N 3 SS 3 3 3SS SP&SSSSS ii i sss J =ss5SSs ^HH 1 H H H HH H HH B3 f i S H i 8 424 1 424 1 1518 1 571 1 535 1 459 1 397 1 537 1 1325 1 H*4 H H H H rt W H riH W H H H I3 1 SS3 3 333 *s3eiiii. 6.805 6.883 7.310 6.981* 6.823 6.895 7.326 6.916 7.316 .w 7.008 7.512 7.32 7.353 7.355 7.368 7.352 7.302 7.275 7.319 i o E S S s aassH. r-r-t r-r-r :-r- s 299.00 7 7 7 299.00 7 7 OlOOOOOOOO O1O0109OOOOO O o o o o o i OM01OO0101OO O 01 o<r>t 3M <-tN flO rl H N N N 01O O O O O O O O 0 1 O 0 IO 1 O O O O O H N 0N A A A A H N ifl H a ii aaaaa ! aaaal ,, g o i ilis Hiiiii mii I 14*M 'f g g g S ^ i S S S S S S ' S S S a s a S S i a s a5 55 5 a s5 a s a S 5 S S S S S 5 S S 3 I " a a a s s s s s s a s a a a s a s s s a s s a s s s s s s a s s s s s s s s s s s s s i s ^ s , 041098:: quantitatad and printad 03/08/99 by drb i f i 6 1 275 1 372 1 1 1 727 1 311 1 301 1 266 1 329 1 <18 1 173 1 281 1 15 1 260 1 352 1 500 1 163 1 257 1 218 1 222 1 226 1 196 1 190 1 119 1 209 1 393 1 535 1 703 1 McUlin*041098: : quantitated and printed 03/08/99 by drb o-- cO O A O H r* N N < 1H o " rO% s Ui ^ o 90 p 1 * n 09 o .i s i z i s SSSS SgS ssssss 3 s s s s s sa.P.s a s s s a a s s a s S S ? asa a 3S| sissass saasss sssxas sss sss i i hhhhhh RSSS 01 0101H A n N < P* 0 0 ^ in m ORA H O A t n O i 9 0 a 0 t H H H O n ifl .O flH O N A M D p R io in r t n a i in to 0 nN H N O R n i r - io co *e 0> 0 i u i u i S CD 0 M O NAO r - o io 01 0 ) 01 0 0 )0 O O 0) 0 wo 01 IO 01 0 HN NA M NO AA A A Aa H NU) R H -4bi i S '" ' 7 7 5S3 aaass sSssE r tf- 3 *> 2 0 0 me 0 i nw op 01 01 01 01 01 Hllllllililllllllllsllllllsllllllslllslllslliillliili lili a 30 O X O i s orl ? 323 3 i 3 !P i * l * 1 ! 33^ 1 s s a a a a a a a a 3 a "a a a S aa ^ 7 7 ^ i N N f j N N p N N N N N O n m H n M M N OjO u iiiiiS iiii |1 ^ l ^ I | i | l ^ S ^ g S l s S S a a S l4- - 3 S S S 5 3 S 3 3 S S aa SS 3S 5S5S33gaaSSSSSSSS333a ag s S S S aS sa gS gS aS gassasjags33S3SS WS SS SS S 5S 5 v 'asassssRsssasssssRsssssssssRssssassssssssass:! 4 * aVM^J BACK TO MAIN Quantify Ccapound Samaxy Raport TOX 120 DUO Rat U v i t Group U S Saapl* List: M:\RATS.PBj0\SMvlaDB\030199o Last nodifiod: Mon Mar 08 09:49:28 1999 Mathod: M:\rats. PRO\MathDB\030199o Last nodifiod: Mon Mar 08 10:03:92 1999 Job Coda: Printad: Mon Iter 08 10:35:49 1999 Compound 8: ?alaar969 Sana laaplaliat Tart 030199001 MaOK Blank 030199002 MaOH Blank 3 030199003 Tor 120, U U 2 2 2 9 - H 2 0 Blk-1, 2/22/99 030199004 Tox 120, BBL0222-8BLivarBlk-l, 2/22/99 S 030199005 Tor 120, KBM222-5ppb-l, 2/22/99 i 030199006 Tor 120, *BL02229-10ppb-l, 2/22/99 7 030199007 Tox 120, 2BL02229-25ppb-l, 2/22/99 0 030199000 Tox 120, RBL02229-50ppb-l, 2/22/99 9 030199009 Tox 120, *BL0222-100ppb-l, 2/2?/ 10 030199010 Tox 120, RBL02229-250ppb-l, 2/22/99 11 030199011 Tox 120, RBL02229-500ppb-l, 2/22/99 12 030199012 Tox 120, BBL02229-750ppb-l, 2/22/99 13 030199013 Tox 120, *BL02229-1000ppb-i, -2/22/99 II 03019901 MaOH Blank 15 030199015 Tax 120 m O . 125F-G2, 2/22/99 05 16 030199016 Tox 120 OHO. 126F-62, 2/22/99 D5 17 030199017 Tox 120 DUO. 1277-G2 2/22/99 D5 18 030199018 Tox 120 m o . 129F-63, 2/22/99 02 19 030199019 Tox 120 rno. 1307-63, 2/22/99 02 20 030199020 Tox 120, RBL02229-250ppb-CCl, 2/22/99 21 030199021 MaOH Blank 22 030199022 Tox 120 OHO. 1317-63, 2/22/99 02 23 030199023 Tox 120 OHO. 1327-63, 2/22/99 D2 21 030199024 Tox 120 OHO. 1337-63, 2/22/99 D2 25 030199025 Tox 120 OKO. 1347-63, 2/22/99 D2 26 030199026 . Tox 120 m o . 135F-63, 2/22/99 D2 27 030199027 Tox 120, RBL02229-250ppb-CC2, 2/22/99 28 030199028 HOOK Blank 29 030199029 Tox 120 m o . 1367-63, 2/22/99 D2 30 030199030 Tox 120 m o . 1377-63, 2/22/99 02 31 030199031 Tox 120 OHO. 1307-63, 2/22/99 02 32 030199032 Tox 120 OHO. 1397-63, 2/22/99 D2 33 030199033 Tox 120 DNO. 1417-63, 2/22/99 02 34 030199034 Tox 120, RBL02229-250ppb-CC3, 2/22/99 35 030199035 MaOH Blank 36 030199036 Tax 120 m o . 1427-63, 2/22/99 02 37 030199037 . Tok 120 OHO. 1437-63, 2/22/99 02 38 030199038 Tox 120, HBLQ2229-250ppb-CC4, 2/22/99 39 030199039 MaOH Blank 40 030199040 Tox 120, 106F-61-250ppb-MS, 2/22/99 41 030199041 Tox 120, 106F-61-250ppb-HSD, 2/22/99 42 030199042 Tox 120, HB02229-250ppb-CCS, 2/22/99 43 030199043 MaOH Blank 44 030199044 Tox 120, RBL02229-H2O Blk-2, 2/22/99 45 030199045 Tox 120, BBX02229-BBLivarBlk-l, 2/22/99 46 030199046 Tox 120, BBL02229-5ppb-2, 2/22/99 47 030199047 48 030199048 Tox 120, RBL02229-10ppb-2, 2/22/99 Tox 120, RB1.02229-25ppb-2, 2/22/99 49 030199049 Tox 120, *BBL02229-50ppb-2, 2/22/99 SO 030199050 Tox 120, RBL02229-100ppb-2, 2/22/99 51 030199051 Tox 120, HBL02229-250ppb-2, 2/22/99 52 030199052 Tax 120, BBL02229-500ppb-2, 2/22/99 53 030199053 Tax 120, RBL02229-750ppb-2, 2/22/99 54 030199054 Tox 120, BBL02229-1000ppb-2, 2/22/99 . Paga 8 Typa Analyt Analyt Analyt Analyt Stand Stand Stand Stand Stand Stand Stand Stand Stand Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt QC Analyt Analyt Analyt QC Analyt Analyt Analyt Standa Standa Stand Stand Stand Standa Standa Standa Standa ltd Cono 5.99 12.00 29.90 59.90 120.00 299.00 599.00 090.00 1190.00 9.414 9.429 9.414 9.460 9.424 9.401 299.00 9.469 9.419 299.00 9.422 299.00 9.428 9.444 9.427 9.420 299.00 9.429 299.00 9.443 9.427 9.450 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 9.407 9.431 9.413 9.426 9.414 9.454 9.440 As m Dil.raot. Cano Tlags %Dv 117 1 238 1 528 1 896 1 1478 1 1532 1 56.03 bb 111.01 bb 230.62 bbX 399.67 bbX 654.20 bbX 677.92 bbX -2 -7 -20 -33 -27 -43 354 1 105 1 507 1 . 162.7 bb -46 53.86 bb 229.52 M M -23 56 1 32.09 M M 4 1 29.38 MM 481 1 . 218.05 bb -27 48 1 544 1 446 1 357 1 337 1 28.51 MM 245.83 bb 203.94 bb 163.92 M M 155.01 bb -18 -48 `5. . 64 1 .132 1 242 1 676 1 ($6 1 1154 1 172 1 36.56 MM 66.50 bb 113.63 bb 393.37 bb 399/62 bbX 512.64 bbX 764.09 bbX 19 9 -5 1 -33 -43 -36 aadUna041098:: quantitatad and printad 03/08/99 by drb BACK TO MAIN Quantify C o ^ a u n d S u u a r y apart TOX 120 DUO Kat Livor Croups 2 fi 3 spia List: M:\RAXS.PBO\SssplsCB\030U9a L u t asdiXiad: Man Iter 08 09:49:26 1999 Mathod: M:\rats.9KO\MstbDB\030199a L u t wxllfi.d: Man M u 08 10:03:52 1999 Jab Coda: Printad: Man M U 08 10:35:49 1999 Caspound 7: surr Ki m 1 030199001 2 030199002 3 030199003 4 030199004 S 030199005 6 030199006 7 030199007 8 030199008 9 030199009 10 030199010 11 030199011 12 030199012 13 030199013 II 030199014 15 030199015 16 030199016 17 030199017 18 030199018 19 030199019 20 030199020 21 030199021 22 030199022 23 030199023 21 03019924 25 030199025 25 030199026 27 030199027 28 030199028 29 030199029 30 030199030 31 030199031 32 030199032 33 030199033 31 030199031 35 030199035 36 030199036 37 030199037 38 030199038 39 030199039 10 030199010 11 030199041 12 030199012 13 030199013 11 030199011 15 030199015 16 030199016 17 030199047 <0 030199048 19 030199049 50 030199050 51 030199051 52 030199052 53 030199053 51 030199051 Sanplolist Tort MaOH Blank MaOH Blank Tox 120, KBL02229-H2O Blk-1, 2/22/99 Tox 120, BBL02229-BBLivarBlk-l, 2/22/99 Tox 120, RBL02229-5ppb-l, 2/22/99 Tox 120, RBL02229-10ppb-l, 2/22/99 Tox 120, 8BL02229-2$ppb-l, 2/22/99 Tox 120, BBL02229-50ppb-l, 2/22/99 Tox 120, BBL02229-100ppb-l, 2/22/99 Tox 120, BBL02229-250ppb-l, 2/22/99 Tox 120, RBL02229-500ppb-l, 2/22/99 Tox 120, BBL02229-750ppb-l, 2/22/99 Tox 120, BBL02229-1000ppb-l, 2/22/99 ' MaOH Blank Toa 120 ONO. 125P-C2. 2/22/99 BS . Tax 120 IMO. 126P-62, 2/22/99 DS Tox 120 IMO. 1271-62, 2/22/99 SS Tox 120 ONO. 1291-63, 2/22/99 D2 Tox 120 OHO. 1301-63, 2/22/99 02 Tox 120, RSL02229-2S0ppb-CCl, 2/22/99 MaOK Blank Tox 120 ONO. 131P-63, 2/22/99 D2 Tox 120 ONO. 132P-63, 2/22/99 D2 Tox 120 ONO. 1332-63, 2/22/99 D2 Tox 120 ONO. 1342-63, 2/22/99 02. .Tox 120 ONO. 1352-63, 2/22/99 02 Tox 120, K8L02Z29-250ppb-CC2, 2/22/99 KaOH Blank ' Tox 120 ONO. .1362-63, 2/22/99 D2 Tox 120 ONO. 1372-63, 2/22/99 D2 Tox 120 OHO. 1382-63, 2/22/99 02 Tox 120 ONO. 1392-63, 2/22/99 02 Tox 120 ONO. 1412-63, 2/22/99 02 Tox 120, BBL02229-250ppb-CC3, 2/22/99 . MaOH Blank Tox 120 ONO. 1122-63, 2/22/99 02 Tox 120 ONO. 1132-63, 2/22/99 02 Tox 120, BBL02229-250ppb-CC4, 2/22/99 MaOH Blank Tox 120, 1062-61-2SOppb-M5, 2/22/99 Tox 120, 1062-61-250ppb-MSD, 2/22/99 Tox 120, RBL02229-250ppb-CCS, 2/22/99 MaOH Blank Tox 120. RBL02229-H2O Blk-2, 2/22/99 Tox 120, SBL02229-*BLivarBlk-l, 2/22/99 Tox 120, BBL02229-5ppb-2, 2/22/99 Tox 120, BBL02229-10ppb-2, 2/22/99 Tox 120, BBL02229-25ppb-2, 2/22/99 TOX 120, KBL02229-50ppb-2, 2/22/99 Tox 120. RBL02229-100])pl>-2, 2/22/99 Tox 120, BBL02229-2S0ppb-2, 2/22/99 Tox 120, BBL02229-500ppb-2, 2/22/99 Tox 120, RBL02229-?50ppb-2, 2/22/99 Tox 120, 8BL02229-1000ppb-2, 2/22/99 Pago 7 Typ Analyt Analyt Analyt Analyt Stand Stand Stand Stand Stand Stand Stand Stand Stand Anailyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt QC Analyt Analyt Analyt QC Analyt Analyt Analyt Stand Stand Stand Stand Stand Stand Stand Stand Stand Std Cono 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 RT 7.334 7.344 7.275 7.277 7.302 7.303 7.315 7.299 7.249 7.308 3.297 7.258 7.293 7.215 7.275 7.284 7.302 7.286 7-300 7.281 7.295 7.287 7.252 7.234 At m DU.Saot. Cane Flag %Dv 494 1 579 1 1017 1 1248 1 1111 1 1149 1 1240 1 1233 1 1192 1 1245 1 1117 1 0.11 M 0.18 MM 0.85 MM 1.04 MM 0.92 MM 0.95 MM 1.03 MM 1.02 m 0.99 M K 1.03 MM 0.93 M M -59 -52 -15 4 -8 -5 3 2 -1 3 -7 908 1 0.75 M M -25 1063 1 191 1 0.88 M M -12 0.11 M M -59 1010 1 1172 1 1377 1 1293 1 1141 1 1108 1 1311 1 1198 1 958 1 .1229 1 .'V 0.81 MM 0.97 Hk 1.11 HM 1.07 HK 0.95 MM 1.17 HM 1.11 MH 1.00 HM 0.80 HM 1.02 MM . -16 -3 11 7 -s' 17 11 0 -26 2 adal1na041098: : quantitatad and printad 03/06/99 by drb BACK TO MAIN 1 _ _ _ _ _ _ _ _ _ :_ _ _ _ _ _ _ 1 _ _ _ _ _ _ _ _ _ _ _ n -* Quantify Calibration Itapoit 5S* 1 , TOX 120 EMO Hat Livax Occupa 2 0 3 . I Calibration: K:\rata.9M0\CumflB\030199a I La*t nodlfiad: Men Mac 08 10:10:07 198 Printed: Mon Mar 01 10:36:09 199 '' 1 ] 1 T 1 i J 3 ] j aadellneO41098;: quantitat*d and printed 03/08/99 by deb Quantify Calibration taport IOX 120 DM0 tut tirar Croup 2 C 3 Calibration: M:\rate.M0\CurvaCB\030199o l u t nodifiad: Mon Mar 08 11:06:52 1999 Printed: Mon Mar OS 11:10:07 I M O . Com pound 2 name: PFH S . C orrelation co efficien t r = 0.999248, r* 2 = 0.998498 C alibration curve: 20.5223 * x + 973922 R esponse type: E xternal Std, A rea C urve type: L inear, O rigin: E x d u d e, W eighting: N ull, A xis trans: N one 3 ] I 1 J I ] I 1 ! I t I udallzw041098: : quantittad and prinfcad 03/08/99 by drb BACK TO MAIN Paga 2 ' ' 1 Quantify Calibration ftaport TCX 120 DHO Rat Livar Group* 2 fi 3 1 Calibration: M:\rats .PRO\CurrCB\030199 Last nodifiad: Mo n Mar 08 10:10:07 1999 Printed: Mon Mas 08 10:36:05 1999 1 I ] ] 1 T 1 I 1 J , ] ] J J 1 ' . BACK TO MAIN * . 1 Quantify Calibration Report TQX 120 DHO Bat Liver Groups 2 3 Calibration: M: \rata .SRO\CurvaDB\030199o 1 Last modified; Mon Mar 08 10:10:07 1999 printed: Mon Mar 08 10:38:05 1999 1 Com pound 4 name: POAA C orrelation co efficien t t = 0.998817, r* 2 = 0.997636 ' C alibration curve: 5*20938 * x + 18.1144 lesp o n se type: E xternal Six], A rea C urve type: Linear, O rigin: Exclude, W eighting: N u ll, A xis tran s: N one J 1 J 3 ] 1 i j I aadallna041098: : quantitated and printed 03/0*/ by dtb > BACK TO MAIN Quantify Calibration fapect TOX 120 ta(0 Rat Hirer Groins ] ( ) Calibration: M:\rats.PR0\CttrvaDB\0J0199o last Modified: Mon Mar 08 10:10:07 1098 Printed: Mon M u 08 10:38:05 1989 - ] ] T i J ] ] 1 I 1 i diline04i098;t quantitated and printed 03/08/99 by dtb BACK TO MAIN Paga 5 . ' 1 Quantify Callbration Baport TQX 120 DKO Kat l i m Sroups I I I ] Calibratimi M:\rats.M0\CusvaEB\O3O199a last aodifiad: Mon Mas 00 10:10:07 190 Psintad: M m Mas 09 10:39:05 199 1 J 1 1 ] J ] ] I I i i I BACK TO MAIN 9*9 * . ' . Quantify Calibration JLaport TOX 120 DUO Rat Livar Group* 2 1 ] Caliteation: M:\sats.8MO\CurraDB\010199a l u t aodifiod: Mon Mar 08 10:10:07 1899 Pxintod: Meo Mas 08 10:38:05 1999 I - . 1 ] 1 I i ! J 1 J J I ] I ' BACK TO MAIN pm 7 * BACK TO MAIN I ] 1 1 i J 3 Method Report Method file: Last nodi fled: Compound: Printed: K:\rats.FItO\HethDB\030199c.sdb Mon Mar 08 10:03:52 19990 1: FFOS Mon Mar 08 10:40:02 1999 Compound Heme: Internal Ref.: Quantify Trace: Cone, of Standards: User UP Valu: . User Peak Factor: Integrate Window Factor: Peak Location FFOS [None] TIC Cone. A 1.000000, Disabled 1.000000 2.000 Retention Time (mine): Relative Retention Time: Time Window (mine): * 0.130, Inablad 0.000, Disabled 0.793 Fak Selection: Response Response Type: Response: eareat . . External Areas Calibration Curves Polynomial Type: Point of Origin: Pit Weighting: Axis Transformation: Linear Exclude None None . Concentration Units: Noise * Feek-to-peak aaplitude: Smooth ' 150 Smoothing: Window size (scans): Number of smooths: ' Smoothing method: Baselines Disabled 3 2 Join vallay threshold: Reduce pak tailing: Raisa baseline aaximin:: Peak Separation Draw vertical threshold: Detect shoulders: Shoulder threshold: * 20.00 100.00 0.00 10.00 Disabled 10.00 ' ` Reasons Thrashold Relative height: Absolute height: Relative eree: Absolute area: . 1.50, Disabled 0.00, Disabled 2.00/ Disabled 0.00, Inablad ' ' Pag 1 M r i d L ___ o ^ m .. LAc m l n s k l 1 1 J 1 Method Report Method fil*: Last modified: Compound: Printed: M:\rats.PRO\MethDB\030199e.mdb Mon Mar 08 10:03:52 19990 2: PPHS Hon Mar 08 10:10:02 1999 Compound N o w : Internal Ref.: Quantify Trace: Cone, of Standards: PFH3 {None] TIC Cone. A ' User W Value: User Peak Factor: Integrate Window Factor: 1.000000, Disabled 1.000000 2.000 Peak Location Retention Tine (Bins): Relative Retention Tine: Tine Window (Bins): * 5.732, Enabled 0.000, Disabled 1.189 Peak Selection: Yearest Response Response Type: Response: External Areas Calibration Curves Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation: Linear Exclude Kona Yon* Concentration Units: Noia* P*ak~to~p*ak amplitude: 290 Smooth Smoothing: Window size (scans):. Number of smooths: Smoothing method: Disabled 3 2 Baselines Join valley threshold: Reduce peak tailing: Raise baseline Baxiaum: ' 0 .0 0 100.00 0.00 Peak Separation Drew vertical three] .. 10.00 Detact shoulders: * Disabled Shoulder threshold: 40.00 Response Threshold Relative height: Absolute height: Reletive area: Absolute area: 1.50, Disabled 0.00, Disabled 2.00, 'Disabled 80.00, Enabled BACK TO MAIN Page 2 HhlJiVif. O M I O V IA c , Oj/oiAfl 1 1 | J ' 1 I 1 1 1 1 fl ... I 1 [ |; ' 1 } j j I 1 J i ___________________________________ :____________________________- Mathod Raport ' Mathod fila: Last Bodifiad: M:\rats.FRO\HathDB\030199c.adb Mon ltar 08 10:03:52 1999Q ' Copound: 3: Printed: Compound Kim : Internal Ref.: Quantify Trace: Cone, of Standards: [None] TIC Cone. A ' m J>L User Kf Value: User Peak Factor: Integrate Window Factor: 1.000000, Disabled 1.000000 2.000 Peak Location Ratantion Tima tains): Ralmtiva Ratantion Tiaa: Tiaa Window (nins): 1.183, Vnabled 0.000, Disabled 1.000 Faak Salaction: Response Response Type: Response: Nearest Xxtemal Areas Calibration Curves Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation: Linear Xsr.lurie None None Concentration Units: * Noise Faak-to-paak amplitude: Smooth 169 Smoothing: Window size (scans): Number of smooths: * Smoothing method: . Disabled 3 2 Baselines Join valley threshold: Reduce peak tailing: *Raise baseline maximum: 2100.00.000 0.00 Faak Senaraticn Draw vertical thresholds Detect shoulders: Shoulder threshold: *RasDOnsa Threshold 10.00 Disabled 0.00 Relative height: Absolute height: Relative area: Absolute area: 1.50, Disabled 0.00, Dlaablad 2.00, -Dlaablad 00.00, Rnablad . cmm I I BACK TO MAIN ,- Pag 3 ' ' jMuMilihi ! ] i ] 1 ] 1 1 ! i i J 3 H.thod R.port M.thod ill.: Last nodifi.d: Compound: Prlntd: M:\rats.PR0\H.thDB\030199o.adb Mbit H u 08 10:03152 1999D 1: M M Mon M*r 08 10:80:02 1 9 Compound Kane: Internal Ref.: Quantify Trace: Cone, of Standards: PQAA [None] . TIC Cone. A Uaar HP Valua: Uaar Paak factor: Intagrata Window Factor: 1.000000, Sisabl 1.000000 2.000 Peak Location Retention Time (mins): Relative Retention Time: Time Window (mins): * 6.628, Xnablad 0.000, Disabled 1.000 Psak Selection: Nearest Response ' Response Type: Response: Kxtemal Areas Calibration Curves Polynomial Type: Point of Origin: Pit Weighting: Axis Transformation: Linear Exclude None None Concentration Units: Noise Peak-to-peak amplitude: 187 Smooth Smoothing: Window size (scans): . Number of smooths: Smoothing method: Disabltd 3 2 Baselines Join valley threshold: Reduce peak tailing:. Raisa basalina maximum:.* 20.00 100.00 0.00 | n k Separation Draw vertical threshold: Detect shoulders: Shoulder threshold: 10.00 * Disabled . 40.00 Response Threshold Relative height: Absolute height: Relative area: Absolute area: 1.5*0, Disabled '0.00, Disabled 2.00, 'Disabled 80.00, Disabled BACK TO MAIN Pag. 8 HarkL. m P M l A c m M n ' 2___________ ** I 1 1 ! 1 ] 1 1 Method Report Method file: Last aodifiad: Coepound: Printed: M:\rata.PRO\MethDB\030199e.adb Mon Mar 00 10:03:32 19990 3: TelnertM Mon Mar 00 10:10:02 1999 Compound Nan*: Internal Rtf.: Quantify Traca: Cene, of Standards: , Talaart69 [Kona] TIC Cene. A . Usar II Vala: Usar Peak Factor: Integrate Window Factor: 1.000000, Disabled 1.000000 2 .0 0 0 Peak location Retention Tine (nina): Relative Retention Tine: Tina Window (Bins): ' 0.370, Inabled 0.000, Disabled 1.000 Peak Selection: Nearest Response Response Types Responses ` *. Sxternal Areas Calibration Curves Polynomial Types Point of Origins Fit Weighting: Axis Transformation: Linear Kxelude None None Concentration Units: Noise Peak-to-peak amplitude: 169 Smooth Smoothing: Window size (scans):. Number of smooths: Smoothing method: Disabled 3 2 Baselines Join valley threshold: Reduce peak tailing: Raise baseline maximum:.* 20.00 100.00 0.00 Peek Seoeratien Drew vertical thresholds Detect shoulders: Shoulder threshold: 10.00 * Disabled _ 40.00 Response Threshold Relative height: Absolute height: Relative area: Absolute area: 1.30, Disabled 0.00, Dieabled 2.00,' Disabled 10.00, Inabled BACK TO MAIN ' M i n i. OHlVL lACs t o U t f Page 3 ** Vt * 1 i i 1 I 1 ] 1 I I J Method Report Method file; Last Modified: Compound: Printed: - M:\rets.PRQ\M.thDB\03al99c.db Mon Mar 08 10:03:52 19990 6: TelaerS69 Hon Mar 08 10:10:02 1999 Compound Kama: Internal Ref.: Quantify Traea: Cone, of Standard: Talaar569 [Hone] TIC Cone. A User RF Value: User Peak factor: Integrate Window factor: Peak Location 1.000000, Disabled 1.000000 2.000 Retention Tiae (sdns): Relative Retention Tine: Tine Window (sins): 9.(18, Knebled 0.000, Disebled 0.500 Peak Selection: Xearest Response Response Typo: Response: # External Areas Calibration Curves Polynomial Typa: Point of Origin: Fit Weighting: Axis Transformation: Linear Sxclude Hone Hone Concantration Units: * Hoisa Peak-to-peak amplitude: , 169 Smooth Smoothings Window size (scans): Humber of smooths: * Smoothing method: Disabled 3 2 e Basalinas .* Join valley threshold: Reduce peak tailing: Raise baseline neslwin: 20.00 100.00 0.00 Peak Separation Draw vertical thresholds Detect shoulders: Shoulder threshold: 10.00 t Disabled 40.00 Response Threshold Relative height: Absolute height: Relative area: Absolute area: 1.50, Disabled 0.00, Disebled 2.00,.Disabled 00.00, Inabled BACK TO MAIN Page 6 __CHUM IO Qllot fa l 1 I 1 1 1 1 J 3 Method Report Mathod fila: Last modiflad: Coapound: Printed: H:\rats.PRO\MethDB\030199c.ndb M n t t a r O I 10:03:52 19*90 7: surr Man Mar 00 10:10:02 1999 Compound Mama: Internal Kaf.t Quantify Traca: Cone, of Standards: surr plana] TIC Fiaod Umar RF Valua: Usax Paalc Factor: ' Xntagrata Window Factors 1.000000, Disabl 1.000000 2.000 Paalc Location Retention line (mins): Relative Retention Tina: Tine Window (nine): Peak Selection: 7.300, Inablad 0.00, Disabled 1.000 Rearest Rasoonsa Rasponsa Typas Rasponsa: Xxtarnal Arm Calibration Curvas Polynomial Type: Point of Origin: Pit Weighting: Axis Tran:formation: T.tnsar Xxcluda Mona Mona Concantration Units: * Kolas Paak-to-paalc amplituda: 169 Smooth Smoothing: Window lira (scans): Nunbar of smooths: ' Smoothing mathod: Disabled 3 2 Basalinas Join valley threshold: Reduce peak tailing: Raise baseline nasiana: Paalc Saturation Drew vertical threshold: Detect shoulders: Shoulder threshold: , 20.00 100.00 0.00 10. Disabled 10.00 Response Threshold ' Ralativa haight: Absoluta haight: Ralativa araa: Absoluta araa: 1.50, Disabled 0.00, Disabled 2.00,. Disabled 00.00, Inabled BACK TO MAIN ' . 11 ` M aA elu. OMHM IAL D l i b s m Paga 7 * AMELIA062498: FACT Equipment Log 98061 Q uattro H , #6448E PAGE DATE: 03/16/99 fas ANALYST: hoi STUDY NUMBER tox!20. f MATRIX: R at liver DESCRIPTION: ion pair dilutions FACT M ethod: FACT-M-4.0 Injection vftlm ne.10 uL Cycle tim e: 13.5 m in. M obile Phase: (A)2.0 mM NH4CH3CQ2 TN-A- or stndrd num ber (A ) 98094-15-20________ Prepared (date/by): (A) 3/16/99 hoj_________ Expires: , (A) 09/99______________ Store at room tem p? Q o r N O ther: Electrode: Z-Spray ffilM eO H (B) TN-A-2325 (B) 3/16/99 hoi (B) 9/16/99 G u ard colum n: N one__________ P art num ber na_________ Lot number na A nalytical colum n: keystone bctasil__________ Size: 2 x 100mm________________ Type: c!8 Sphere size: 5 um Part num ber 105-701-2 L ot nnmher P8HQ8 Serial num ber 029495D A nalytical param eters: Drying gas (L/hr): 300 ESI nebulizing gas fL/hr) 15 LC Flow rate: 300 uL/mm. Bade pressure: 100 bar Sheath gas (L/hr)_0_ H PLC solvent gradient: long! Tim e (m in.) 1) 0.00 U ! _____ 3 11.0 5) 12:5 ' 6 ) 13.5 %B 40 90 90 40 40 BACK TO MAIN 1 ^ ?Ufa_______ ;______________ Tuning Method Report 1 Method: C :\MASSLYNX\RATS2. PRO\ACQUDB\PFOS 1 Printed: Tue Mar 16 09:59:43 1999 ___ ___________________________________ __________________ .) Afrdia O L M W BACK TO MAIN Page 1 SOURCE ( ESP- ) ] Capillary Cone . J Extractor RF Lens Source Block Temp. Desolvation Temp. 1 ] Pressures * Analyser Vacuum 1 Gas Cell Set Rdbk 3.00 60 5 0.2 150 .250 2.99 -60 -6 150 258 Rdbk 1.0e-4 4.5e-3 MSI LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier Set Rdbk 15.0 15.0 2.0 1 . 0.0 56 650 Set Rdbk 15.0 15.0 45 2.0 0.0 40 0 650 46 38 46 BACK TO MAIN ik k _ _ _ _ _ _ _ _ 1 I _ _ _ _ _ _ =_ _ Scanning Nathed Raporfe Mathedi C:\KXSSmOC\BXIM. PRO\ACOUDB\PFOS-PrHS-IS Last Modified: Mon Mar IS 13:23:33 1*9 Printad: Tua Mas IS 10:53:91 1999 Amila M b os Solvant Balay ( aina ) : 0.00 punetlon : 1 M M of 4 Stesa Paisa iI ESP- ) ' Intar Channal Daisy ( Sacs ) i Span ( Daltons ) : Start Tlaa ( Kina ) : End T i m ( Kina ) i Rapaata : Channal Parant Daughtar 0.03 0.00 0.00 13.SO 1 Duali (Saca) Cell Enargy (eV) Cone C v ) 1 499.00 80.00 0.40 45 60 2 499.00 99.00 0.40 4S 60 3 499.00 130.00 0.40 45 60 4 499.00 180.00 0.40 45 60 Function : 2 M M of 4 Masa Paisa iC ESP- ) Intar Channal Daisy 1 Saca ) t Span ( Daltons ) : Start Tina I Mins 1 t End Tina ( Hina ) : Rapaata : . Channal Parant Daughtar 0.03 0.00 0.00 13.50 1 Duali . (Saca) Coll Enargy (eV) * Cena ( V ) 1 399.00 *80.00 0.40 2 399.00 99.00 0.40 3 399.00 130.00 0.40 4 399.00 180.00 0.40 45 45 45 45 60 60 60 60 :_ _ i _ Paga 1 function s 9 M M of 1 N u i Pais 1 ESP- ) . Intar Channel Delay ( Seca ) i Span ( Daltons ) : Start Tina ( Mina ) : End Tine ( Hina ) : Rapaata : . Channel Parent Daughtar 0.03 0.00 0.00 13.50 1 Dwell (Saca) Coll Enargy (aV) Cone ( V ) 1 427.00 80.00 0.40 40 60 BACK TO MAIN 3 It fa r____________ .________________________ __________________ ,_____________ _1 Masslynx - Sample List Page 1 Sample List C:\MASSLYNX\rats2.PRO\SampleDB\031699b.SPL , Printed:TueM ar 1614:40:111S99______________________ I m L D I.M J U it /, File Name File Text MS R ie Inlet File B o ttle 1 031699001 MeOH Blank PFOS-PFHS-IS long2 91 2 031699002 MeOH Blank PFOS-PFHS-IS long2 91 3 031699003 Tox120, RBL02229-H2OBIk-2,2/22/99 PFOS-PFHS-IS k>ng2 1 4 031699004 Tox120, RBl02229-UvBlk-2,2/22/99 PFOS-PFHS-IS long2 2 5 031699005 Tox120, RBL02229-5ppb-2,2/22/99 PFOS-PFHS-IS long2 3 6 031699006 Tox120, RBL02229-10ppb-2,2/22/99 PFOS-PFHS-IS long2 4 7 031699007 Tox120, RBL02229-25ppb-2,2/22/99 PFOS-PFHS-IS long2 5 8 031699008 Tox120, RBL02229-50ppb-2,2/22/99 PFOS-PFHS-IS long2 6 9 031699009 Tox120, RBL02229-100ppb-2,2/22/99 PFOS-PFHS-IS bng2 7 10 031699010 TOX120, RBL02229-250ppb-2,2/22/99 PFOS-PFHS-IS long2 8 11 031699011 Tox120. RBL02229-500ppb-2,2/22/99 PFOS-PFHS-IS long2 9 12 031699012 Tox120, RBL02229-750ppb-2,2/22/99 PFOS-PFHS-IS tong2 10 13 031699013 Tox120, RBL02229-1000ppb-2,2/22/99 PFOS-PFHS-IS long2 11 14 031699014 MeOH Blank PFOS-PFHS-IS long2 91 15 031699015 Tox120,161F-G 5,2/22/99, d250 PFOS-PFHS-IS long2 12 16 031699016 Tox120,162F-G5,2/22/99, d250 PFOS-PFHS-IS long2 13 17 031699017 Tox120,163F-G5,2/22/99, d250 PFOS-PFHS-IS long2 14 18 031699018 Tox120,164F-G5,2/22/99, d250 PFOS-PFHS-IS long2 15 19 031699019 TOX120,165F-G5,2/22/99, d250 PFOS-PFHS-IS long2 16 20 031699020 Tox120, RBL02229-250ppb-CC2,2/22/99 PFOS-PFHS-IS long2 82 21 031699021 MeOH Blank PFOS-PFHS-IS long2 91 22 0316*99022 Tox120,166F-G5,2/22/99, d250 PFOS-PFHS-IS long2 17 23 031699023 Tox120,167F-G5,2/22/99, d250 PFOS-PFHS-IS long2 18 24 031699024 Tox120,168F-G5,2/22/99, d250 PFOS-PFHS-IS long2 19 25 031699025 Tox120, RBL02229-250ppb-CC3,2/22/99 PFOS-PFHS-IS long2 83 26 031699026 MeOH Blank PFOS-PFHS-IS long2 92 27 031699027 Tox120,177F-G6,2/22/99, d1000 . PFOS-PFHS-IS long2 20 28 031699028 Tox120,1787F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 21 29 031699029 Tox120,179F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 22 30 031699030 Tox120,180F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 23 31 031699031 TOX120,181F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 24 32 031699032 TOX120, RBL02229-250ppb-CC4,2/22/99 PFOS-PFHS-IS long2 84 33 031699033 MeOH Blank PFOS-PFHS-IS long2 92 34 031699034 Tox120,182F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 . 25 35 031699035 TOX120,183F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 26? 36 031699036 Tox120,184F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 27 37 031699037 Tox120,185F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 28 38 031699038 TOX120,186F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 29 39 031699039 Tox120, RBL02229-250ppb-CC5,2/22/99 PFOS-PFHS-IS long2 85 40 031699040 MeOH Blank PFOS-PFHS-IS long2 92 41 031699041 Tox120,187F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 30 42 031699042 Tox120,189F-G6,2/22/99, d1000 PFOS-PFHS-IS. long2 31 43 031699043 TOX120,190F-G6,2/22/99, d1000 PFOS-PFHS-IS long2 32 44 031699044 Toxl20,191F-G 6,2/22/99, d1000 PFOS-PFHS-IS long2 33 45 031699045 Tox120, RBL02229-250ppb-CC6,2/22/99 PFOS-PFHS-IS long2 86 46 031699046 MeOH Blank PFOS-PFHS-IS long2 93 47 031699047 Tox120.169F-G5,2/22/99 . PFOS-PFHS-IS long2 34 48 031699048 T o x1 20 ,1 70 F -G 5 ,2/22/99 PFOS-PFHS-IS long2 35 49 031699049 T o x1 20 .1 71 F -G 5 ,2/22/99 PFOS-PFHS-IS long2 36 50 031699050 Tox120, RBL02229-250ppb-CC5, 2/22/99 PFOS-PFHS-IS fong2 85 51 031699051 MeOH Blank PFOS-PFHS-IS long2 94 52 031699052 T o x1 20 .1 73 F -G 5 ,2/22/99 PFOS-PFHS-IS long2 37 53 031699053 T o x1 20 .1 74 F -G 5 ,2/22/99 PFOS-PFHS-IS fong2 38 54 031699054 T o x1 20 .1 75 F -G 5 ,2/22/99 PFOS-PFHS-IS bng2 39 55 031699055 Tox120, RBL02229-250ppb-CC6,2/22/99 PFOS-PFHS-IS bng2 86 56 031699056 MeOH Blank PFOS-PFHS-IS bng2 94 57 031699057 Tox120, RBL02229-H2OBIk-2,2/22/99 PFOS-PFHS-IS bng2 1 58 031699058 Tox120, RBL02229-LivBlk-2,2/22/99 PFOS-PFHS-IS bng2 2 59 031699059 *Tox120, RBL02229-5ppb-2,2/22/99 PFOS-PFHS-IS long2 3 60 031699060 Tox120, RBL02229-1Oppb-2,2/22/99 PFOS-PFHS-IS long2 4 Volume Sam 10 Anal 10 Anal 10 Anal 10 Anal 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan . 10 Stan 10 Anal 10 Anal 10 Anal 10 Anal 10 Anal 10 Anal 10 QC 10 . Anal 10 Anal 10 Anal 10 Anal 10 QC 10 Ana; . 10 Ana 10 Ana 10 Ana 10 Ana 10 Ana 10 QC 10 Ana . ' 10 Ana 10 Ana 10 Ana 10 Ana 10 Ana 10 QC 10 Ana 10 Ana 10 Ana 10 Ana 10 Ana 10 QC 10 Ana 10 Ana 10 Are 10 Ana 10 QC 10 Are 10 Are 10 Are 10 Are 10 QC 10 Are 10 Are 10 An; 10 Sta 10 Sta BACK TO MAIN ta\ 3ilefa___;_____ ;______________ __________;_________ Masslynx - Sample List ' Page 2 Sample U s t C:\MASSLYNX\ratsZPRO\SampleDB\Q31699b.SPL Printed: Tue Mar 1614:40:11 1999 \hwliR 0I*2W IAC-0s/lolll File Name 61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067 File Text . TOX120, RBL02229-25ppb-Z2/22/99 Tox120, RBL02229-50ppb-2,2/22/99 Tox120, RBL02229-100ppb-2,2/22/99 Tox120, RBL02229-250ppb-2,2/22/99 Tox120, RBL02229-500ppb-2,202/99 Tox120, RBL02229-750ppb-Z 2/22/99 Tox120, RBL02229-1000ppb-2,2/22/99 MS File PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS Inlet File long2 k>ng2 k>ng2 long2 k>ng2 long2 k>ng2 Bottle 5 6 7 8 9 10 11 Volume Sam 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan I FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN Date: 3/17 Iw 1 J I BACK TO MAIN Quantify Compound Sunnary Raport T0X120 Sat Livor Group 3 and Sanpla Llat: G:\rata2.FBO\8aqplaDB\Q31699b La at nodifind: Wod klar 17 13:54:10 1999 Mathod: G:\rata2.PRO\MathDB\03l699b Last modiflad: Wad Mar 17 06:56:20 1999 Job Coda: . Printad: Wad Mar 17 14:33:58 1999 Compound 1: PTOS Mama 1 031699001 2 031699002 3 031699003 4 031699004 5 031699005 6 031699006 7 031699007 031699008 9 031699009 10 031699010 11 031699011 12 031699012 13 031699013 14 031699014 15 031699015 16 031699016 17 031699017 ia 031699018 19 031699019 20 031699020 21 031699021 22 031699022 23 031699023 24 031699024 25 031699025 26 031699026 27 031699027 2S 031699028 29 031699029 30 031699030 31 031699031 32 031699032 33 031699033 34 031699034 35 031699035 36 031699036 37 031699037 38 031699036 39 031699039 40 031699040 41 031699041 42 031699042 43 031699043 44 031699044 45 031699045 46 031699046 47 031699047 48 031699048 49 031699049 SO 031699050 51 031699051 52 031699052 53 031699053 54 031699054 55 031699055 56 031699056 ' 57 031699057 58 031699058 59 031699059 60 031699060 61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067 luvlaliit Txt K.OH Blank MaOH Blank Toxl20, WL0222S-H20BU-2, 2/22/99 Toxl20, KBL02229-LiYBlk-2, 2/22/99 Texl20, BBL02229-Sppb-2, 2/22/99 TOX120, ML02229-10ppb-2, 2/22/99 TOX120, RBL02229-25ppb-2, 2/22/99 Toxl20, ML02229-50ppb-2, 2/22/99 Toxl20, RBZ.02229-100ppb-2, 2/22/99 Toxl20, RBL02229-250ppb-2, 2/22/99 Toxl20,RBL02229-500ppb-2, 2/22/99 TOX120, ABL02229-750ppb-2, 2/22/99 TOX120, ABL02229-1000ppb-2, 2/22/99 MaOK Blank . Toxl20, Kir-GS, 2/22/99, d2S0 TOX120, 1S2F-GS, 2/22/99, -d250 ' TOX120, 1S3F-G5, 2/22/99, d2S0 Toxl20, 16<r-GS, 2/22/99, d2S0 Toxl20, 16SF-GS, 2/22/99, d250 TOX120, BBLO2229-2S0ppb-CC2, 2/22/99 MaOH Blank TOX120, 1C6F-G5, 2/22/99, <1250 Toxl20, m r - C S , 2/22/99, d250 Toxl20, l(ar-6S, 2/22/99, d250 Toxl20,*RBL02229-2S0ppb-CC3, 2/22/99 MaOH Blank 0 Toxl20, 177r-GS, 2/22/99, dlOOO TOX120, 170XMS, 2/22/99, dlOOO Toxl20, 179r-0S, 2/22/99, dlOOO Toxi20, aor-eo, 2/22/99, diooo Toxl20, 1I1F-G6, 2/22/99, dlOOO TOX120, BBL02229-250ppb-CC4, 2/22/99 MaOH Blank . TOX120, 192r-G5, 2/22/99, dlOOO TOX120, 183F-GS, 2/22/99, dlOOO Toxl20, 1S4F-G6, 2/22/99, dlOOO TOX120, 195F-GS, 2/22/99, dlOOO TOX120, 196F-G6, 2/22/99, dlOOO Toxl20, BBL02229-250ppb-CCS, 2/22/99 MaOH Blank TOX120, 197F-C6, 2/22/99, dlOOO TOX120, 199F-66, 2/22/99, dlOOO Toxl20, 190F-GC, 2/22/99, dlOOO TOX120, 191F-66, 2/22/99, dlOOO TOX120, BBL02229-250ppb-CC6, 2/22/99 MaOH Blank TOX120. 1S9F-G3, 2/22/99 TOX120. 170F-G5, 2/22/99 Toxl20..171F-GS, 2/22/99 Toxl20,' RBL02229-250ppb-CC5, 2/22/99 MaOH Blank TOX120. 173F-6S, 2/22/99 Toxl20. 174F-GS, 2/22/99 IwliO. 175F-GS, 2/22/99 TOX120, RBlO2229-2S0ppb-CC, 2/22/99 MaOH Blank . . Toxl20, BBL02229-H20Blk-2, 2/22/99 TOX120, m 0 2 2 2 9 - U v B l k - 2 , 2/22/99 TOX120, RBIi02229--5ppb-2, 2/22/99 TOX120, BBL02229-10ppb-2, 2/22/9 TOX120, BBL02229-25ppl>-2, 2/22/99 Toxl20, BBL02229-50ppb-2, 2/22/99 TOX120, RBL02229-100ppb-2, 2/22/99 Toxl20, m02229-250ppb-2, 2/22/99 TOX120, RB02229-500ppb-2, 2/22/99 TOX120, HBL02229-750ppb-2, 2/22/99 TOX120, ABl*02229-1000ppb-2, 2/22/99 . . . Pillila . Fypa Analy Analy Analy Analy Stand Stand Stand Stand Stand Stand Stand Stand Stand . Analy Analy Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC Analy Analy . Analy Analy Analy Analy QC' Analy Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Stand Stand Stand Stand Stand Stand Stand Stand Stand 8td Cone 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 299.00 299.00 299.00 299.00 299.00 ST 8.474 9.067 9.026 9.096 9.084 9.033 9.020 8.969 9.029 9.033 9.036 9.034 8.977 8.973 9.103 * 9.060 9.114 9.101 9.058 . 9.070 9.066 9.070 9.068 9.063 8.805 9.066 9.057 9.065 9.060 9.057 9.061 9.056 9.064 9.056 9.051 9.056 9.005 9.062 9.001 9.075 9.032 9.085 9.040 9.03S At m 8749 114 318 378 777 1043 1973 3368 6297 15755 29424 39984 62763 128 253 65 96 128 9673 243 165 262 45 12476 28 18135 18935 19227 18644 20438 11041 45 19755 16765 16092 10923 13246 11816 155 11727 9219 9388 10457 10389 9.031 8.907 8.914 299.00 8.907 8.976 8.975 8.920 .918 299.00 8.908 9.045 1.977 8.971 5.99 8.968 12.00 8.898 29.90 8.967 59.90 8.907 120.00 8.969 299.00 8.910 599.00 8.900 898.00 8.918 1198.00 8.971 3257 3363 2407 13894 121 3473 2677 5396 12660 49 304 329 768 1172 X 1988 3354 6672 15526 29963 43816 62400 Saaponaa 8749.32 114.33 317.81 378.28 777.34 1043.01 1973.20 3368.08 6297.05 15754.60 29423.97 39983.57 62762.84 128.18 253.39 64.63 95.67 128.38 ' 9673.44 242.96 . 164.97 262.30 44.62 12476.30 28.42 18134.95 18935.20 19226.52 18644.16 20437.57 11040.82 45.17 19754.86 16764.61 16091.88 10923.35 13245.65 11815.78 155.06 11727.19 9219.46 9388.04 10457.05 10389.20 3257.20 3362.65 2407.33 13893.83 120.93 3473.23 2677.29 5396.21 12660.21 49.26 303.77 328.82 767.69 1172.49 1988.14 3353.64 6672.19 15526.06 29962.79 43815.88 62399.96 Cono. Plaaa tfiav 172.09 bb 0.00 bb 0.00 bb 0.00 bb 5.39 MM -10 10.94 bb 30.39 bb -9 2 59.56 bb -1 120.81 bb 1 318.57 bb 604.41 MM 7 1 825.22 bb -8 1301.55 bbX 9 0.00 HM 0.00 bb 0.00 bb 0.00 bb 0.00 bb 191.41 MM 0.00 bb 0.00 bb 0.00 bb 0.00 bb 250.02 bb 0.00 bb 368.35 MM 385.08 bb 391.17 bb 379.00 MM 416.50 MM 220.00 KM 0.00 bb 402.22 MM 339.69 Mi 325.63 bb 217.55 MM 266.11 bb 236.21 bb 0.00 bb 234.36 bb 181.92 MM 185.44 KM 207.80 MM 206.38 MM . -36 -16 \ -26 -21 -31 57.24 bb 59.45 bb ,, 39.47 bb 279.66 bb * 0.00 bb 61.76 bb 45.12 bb 101.97 bb 253.87 bb 0.00 bb ' 0.00 bb 0.00 bb 5.19 Ml 13.65 bb 30.71 bb 59.26 bb 128.65 bb 313.79 bb 615.68 bb 905.35 bb 1293.96 bbX -6 -15 -13 14 3 -1 7 5 3 1 8 Amalia 052498 quanti tatad and printad on 03/17/99 by LAC BACK TO MAIN 1 ! ] I ] 1 1 J 1 ] 1 Quantify Ceo^sund Suaaacy M p o r t 10X120 Rat 1*1var Group S and < Sanpl. List! O:\xats2.VRO\Iug>lDBV)31i9b Last andlflad: ad Mac IT 13;S4:10 1*9# Nathod: G:\cata2.PRO\MathDB\031CMb Last aodiflad! Wad Mac IT 0:S:20 1 Job Codas Printad: ad Mac IT 14i33i5( 1999 Compound 2i tucc t Mana 1 031699001 2 031699002 3 031699003 4 031699004 S 031699005 6 031699006 7 031699007 031699008 9 031699009 10 031699010 11 031699011 12 031699012 13 031699013 14 031699014 IS 031699015 16 031699016 17 031699017 18 031699018 19 031699019 20 031699020 21 031699021 22 031699022 23 031699023 24 031699024 25 031699025 26 031699026 27 031699027 28 031699028 29 031699029 30 031699030 31 031699031 32 031699032 33 031699033 34 031699034 35 031699035 36 031699036 37 031699037 38 031699038 39 031699039 40 03169904Q 41 031699041 42 031699042 43 031699043 44 03169904-4 45 031699045 46 031699046 47 031699047 48 031699048 49 031699049 50 031699050 51 031699051 52 031699052 53 031699053 54 031699054 55 031699055 56 031699056 ` 57 031699057 58 031699058 59 031699059 60 031699060 61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067 Saaplallat Taxt HsOR Blank MaOK Blank Toxl20, BBL02229-H20Blk-2, 2/22/99 Toxl20, RBL02229-UvBlk-2, 2/22/99 Toxl20, ABL02229-Sppb-2, 2/22/99 TOX120, RBL02229-10ppb-2. 2/22/99 Toxl20, RBL02229-2Sppb-2, 2/22/99 TOX120, RBL02229-50ppb-2, 2/22/99 TOX120, RBL02229-100ppb-2, 2/22/99 TOX120, BBLO2229-2S0ppb-2, 2/22/99 TOX120/RBL02229-500ppb-2, 2/22/99. Toxl20, RBL02229-T50ppb-2, 2/22/99 TOX120, KBL02229-1000ppb-2, 2/22/99 HaCH Blank TOX120, 161F-G5, 2/22/99, d250 . Toxl20, K2F-GS, 2/22/99, -d2S0 TOX120, 1S3F-GS, 2/22/99, d250 Toxl20, 164F-G5, 2/22/99, d250 TOX120, 165F-GS, 2/22/99, 0250 TOX120, R8L02229-2Sppb-CC2, 2/22/99 MaOH Blank . Toxl20, 1C9F-CS, 2/22/99, d250 TOX120, 1CTF-G5, 2/22/99, d2S0 Toxl20, 1SSF-G5, 2/22/99, (1250 TOX120, RBL02229-2S0ppb-CC3, 2/22/99 MaOH Blank p TOX120, 1TTF-G, 2/22/99, dlOOO Toxl20, 17B/F-G, 2/22/99, dlOOO TOX120, 1T9F-G6, 2/22/99, dlOOO TOX120, 180F-G6, 2/22/99, dlOOO Toxl20, liir-GC, 2/22/99, dlOOO ' TOX120, RBL02229-250ppb-CC4, 2/22/99 MaOH Blank TOX120, 152r-GC, 2/22/99, dlOOO TOX120, 183F-G6, 2/22/99, dlOOO TOX120, 184F-G6, 2/22/99, dlOOO TOX120, 18SF-G8, 2/22/99, dlOOO TOX120, 186r-G6, 2/22/99, dlOOO TOX120, RBL02229-250ppb-CCS, 2/22/99 MaOH Blank TOX120, 18TF-G, 2/22/99, dlOOO TOX120, 189F-G6, 2/22/99, dlOOO TOX120, 190F-G8, 2/22/99, dlOOO Toxl20, 191F-G6, 2/22/99, dlOOO TOX120, BBL02229-250ppb-CC8, 2/22/99 MaOH Blank Toxl20. 169F-GS, 2/22/99 * Toxl20. 170F-G5, 2/22/99 TOX120.-1T1F-GS, 2/22/99 TOX120,' RBL02229-2S0ppb-CCS, 2/22/99 MaOH Blank TOX120. 1T3F-G5, 2/22/99 TOX120. 1T4F-G5, 2/22/99 TOX120. 1T5P-G5, 2/22/99 Toxl20, BBL02229-250ppb-CC6, 2/22/99 MaOH Blank TOX120, RBL02229-H20Blk-2, 2/22/99 TOX120, RBL02229-LivBlk-2, 2/22/99 Toxl20, RBL02229-5ppb-2, 2/22/99 TOX120, RBL02229-10ppb-2, 2/22/99' Toxl20, RBL02229-25ppb-2, 2/22/99 Toxl20, BBL02229-50ppb-2, 2/22/99 TOX120, BBL02229-100ppb-2, 2/22/99 TOX120, BBL02229-250ppb-2, 2/22/99 Toxl20, RBL02229-500ppb-2, 2/22/99 Toxl20, BBL02229-T50ppb*2, 2/22/99 TOX120, BBL02229-1000ppb-2, 2/22/99 ( p ^ ) l A C 0^1 R H * ! ' ' TVpa Analy Analy Analy Analy Stand Stand Stand Stand Stand Stand Stand Stand Stand Analy Analy ` Analy Analy Analy Analy . QC Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC Analy Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy - Analy QC Analy Analy Analy Stand Stand Stand Stassd Stand . Stand Stand Stand Stand Std Cono Kt 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.412 8.404 8.420 8.404 8.354 8.413 8.354 8.358 8.354 8.361 8.356 1.00 8.290 8.378 8.453 1.00 8.37*9 8.384 8.38^ 8.123 1.00 8.380 7.984 1.00 8.446 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 8.355 8.418 B.228 8.235 8.294 8.361 8.303 8.301 8.230 8.359 8.359 8.290 8.285 8.289 8.289 8.357 8.295 8.286 8.304 8.292 ' . tag* 2 _ Aru 684 2274 2907 2500 2189 1931 . 2004 1623 1549 1775 48 8 pona* 684.42 2274.17 2906.68 2499.85 2189.27 1931.02 2004.23 1622.84 1548.68 1774.97 47.58 12 . 737 7. 12.14 736.68 7.06 1174 9 10 14 982 9 1173.91 8.97 9.59 14.36 . 981.80 9.15 955 955.41 Cono. llaga %Dv 0.41 bb 1.37 bb 1.75 bb 1.51 bb 1.32 bb 1.16 bb 1.21 bb 0198 bb 0.93 bb 1.07 bb 0.03 bb -59 37 75 51 32 16 21 -2 -7 7 -97 0.01 bb 0.44 bb 0.00 bb -99 -56 -100 0.71 bb 0.01 bb 0.01 bb 0.01 bb 0.59 bb 0.01 bb -29 -99 -99 -99 -41 -99 0.58 bb -42 741 1213 601 617 809 1236 706 658 623 1229 1222 1139 , 1567 .X 1317 1190 1278 1211 1102 1179 1136 740.87 1213.32 600.53 616.93 809.04 1235.83 706.35 657.72 623.03 1229.39 1221.94 1138.70 1567.05 1317.43 1189.57 1277.63 1211.23 1102.30 1178.81 1135.66 0.45 bb 0,73 bb 0.36 bb * 0.37 bb # 0.49 bb 0.74 bb 0.43 bb 0.40 bb ' 0.38 bb 0.74 bb 0.74 bb 0.69 bb 0.94 bb 0.79 bb 0.72 bb 0.77 bb 0.73 bb 0.66 bb 0.71 bb 0.68 bb -55 -27 * -64 -63 -51 -26 -57 -60 -62 -26 -26 -31 -6 -21 -28 -23 -27 -34 -29 -32 Anclla 062498 quantitated and printad on 03/17/99 by ZAC 1 Quantify Calibration Raport 90X120 Rat Livax Group 8 and 8 Calibration: G:\rata2.PRO\CurvaDB\031S99b Last aodiflad: Wad Mar 17 14:37:10 1999 Printad: Wad Mar 17 14:34:29 1999 . * . BACK TO MAIN Pa 1 # Quantify Calibration Raport TCX120 Rat Livar Group S and ( Calibration: O:\rata2. 9R0\CurvaDB\031C99b Laat aodifiad: Wad Mar 17 14i37<10 199 Printad: Wad Mar 17 14:34>29 1999 ' ' ' ' ' BACK TO MAIN hga 3 Amalia 042499 quantittad and printad an 03/17/99 by LAC 1 Method Report 1 Method file: Last modified: Compound: 0: \rats2. PRO\MethDB\031699b.mdb Wed Mar 17 06:54:20 1999 1: PPOS Printed: Wed Mar 17 13:11:29 1999 1 Compound Name: Internal Ref.: Quantify Traca Cone. o Standards I Usar W Valas Usar Peak Faetor: . Integrate Window Faetor: PPOS [None] TIC Cone. A 1.000000f Disabled 1.000000 2 .0 0 0 . A/nelio. Retention Tina (mine) : Relative Retention Tina: Tine Window (nine) i Peak Selections ] Response Types Response: 8.843, Enabled 0.000, Disabled 0.717 Nearest External . Areas 1 Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation: 7 Units: Peek-to-peak amplitude: Linear Exclude 1/X None 293 Smoothings Window sise (scans) s Number of smooths: Smoothing method: * Disabled 3 2 Join valley thresholds Reduce peak tailing: Raise baseline maxiana: ] Draw vertical threshold: Detect shoulders: Shoulder threshold: J Relative heights Absolute heights Relative areas * Absolute areas 30.00 70.00 4.00 10.00 Disabled 10.00 50.00, Enabled 0.00. Disabled 3.00, Enabled 0.00, Disabled BACK TO MAIN * page : _____ I L l l S ______________ Method Report Method file: Last modified: Compound: print.di a :\r.t,2.PXO\ltethDB\031699b.Mdb N.d M w 17 06:36*20 1003 2: Surr Had Mar 17 11:11:29 1999 Compound Nam* t Internal Ref.: Quantify Traai Cone, of Standards{ Dear RF Values User Peak Factor: Integrate Window Factor: Surr (Non] TIC Fixed * 1 .0 0 0 0 0 0 , D isa b le d 1.000000 2 .0 0 0 Retention Time (mine): 1 Relative Retention Time: Time Window (mins) :. Peak Selection: 3 Response Type: Response: 8.231, Enabled 0.000, Disabled 0.S88 Nearest External . Areas ' Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation: T units: Peak-to-peak asplitude: \ i i i Smoothing: Window size (scans): Number of smooths: i Smoothing method: T.lnoar Exclude None None n Disabled 3 2 Join valley threshold: Reduce peak tailing: t Raise baseline maximum: 30.00 70.00 4.00 ] DraW vertical threshold: 10.00 Detect shoulders: . Disabled Shoulder threshold: J Relative height: * * 10.00 50.00, Enabled Absolute height: 0.00, Disabled Relative area:* 3 .0 0 , BM bled Absolute area: 0.00, Disabled :. A fo tL o t z w BACK TO MAIN Page : ___Ac o iln -jw BACK TO MAIN 1 Quantify Cenpound Suaaary Kapert TOX120 Rat Llvar Qroup 5 and f Vaga 1 1 Baapl. Liat: a:\rata2.Ht0\8anpla0B\031dRtb Laat .edified: Wad Mar IT 13:54)10 19 Matbed: O:\rata2.moVMathDB\031tttb2 laat aodlflad: Wad Mar IT 0(:5:0 1 Job Coda: Printed: ' Wad Mar IT 15:01:13 1 Coapeund 1: PIHf ] ] 1 T iI J 1 ] Ms m s Saaplali.t Text 1 031699001 HeOH Blank 2 031699002 MaOH Blank 3 031699003 Toxl20, BBl02229-H2OBlk-2, 2/22/9 4 031699004 Toxl20, RBL02229-LlvBlk-2, 2/22/9 5 031699005 TOX120, RBU)2229-5ppb-2, 2/22/99 6 031699006 TOX120, R8L02229-10ppb-2, 2/22/99 7 031699007 TOX120, RBL02229-25ppb-2, 2/22/9 031699008 TOX120, RBL02229--50ppb-2, 2/22/99 9 031699009 Toxl20, RBL02229-- 100ppb-2, 2/22/99 10 031699010 TOX120, RBL02229-2S0ppb-2, 2/22/99 11 031699011 TOX120, -RBL02229-500ppb-2, 2/22/9 12 031699012 TOX120, RBL0222-TS0ppb-2, 2/22/99 13 031699013 TOX120, RBL02229-1000ppb-2, 2/22/99 14 031699014 MaOH Blank 15 031699015 TOX120, liir-GS, 2/22/9, d2S0 16 031699016 Toxl20, 192P-65, 2/22/9,.d250 ' 17 031699017 Toxl20, lC3r-G5, 2/22/99, d2S0 It 031699018 TOX120, 14F-G5, 2/22/99, d2S0 19 031699019 Toxl20, KSF-GS, 2/22/99, d250 20 031699020 TOX120, RBL02229-250ppb-CC2, 2/22/99 21 031699021 MaOH Blank 22 031699022 TOX120, 166T-G5, 2/22/99, d250 23 031699023 TOX120, 16T1--G5, 2/22/99, d2S0 24 031699024 Toxl20, ltr-GS, 2/22/9, d2S0 25 031699025 TOX120, RBI.0222-250ppb-CC3, 2/22/99 26 931699026 MaOH Blank In 27 031699027 Toxl20, 1TTF-KSC, 2/22/99, dlOOO 26 031699028 Toxl20, 1T9VT-G6, 2/22/9, dlOOO 29 031699029 TOX120, 1T9F-G6, 2/22/99, dlOOO 30 031699030 Toxl20, 1S0F-GC, 2/22/9, dlOOO 31 031699031 Toxl20, 1S1F-GC, 2/22/9, dlOOO 32 031699032 TOX120, RBL02229-250ppb-CC4, 2/22/99 33 031699033 MaOH Blank . 34 031699034 TOX120, 1S2F-66, 2/22/99, dlOOO 35 031699035 TOX120, 193F-S4, 2/22/99, dlOOO 36 031699036 Toxl20, 194F-GS, 2/22/9, dlOOO 37 031699037 TOX120, 19SF-GC, 2/22/9, dlOOO 38 031699038 Ioxl20, 1IGF-GC, 2/22/9, dlOOO 39 031699039 Toxl20, RBL02229-250ppb-CC5, 2/22/9 40 031699040 MaOH Blank 41 031699041 TOX120, 1ITF-G6, 2/22/99, dlOOO 42 031699042 -TOX120, 199F-GS, 2/22/99, dlOOO 43 031699043 TOX120, 190F-GC, 2/22/99, dlOOO 44 031699044 TOX120, 191F-G6, 2/22/9, dlOOO 45 031699045 Toxl20, BB1.02229-2S0ppb-CCS, 2/22/99 46 031699046 MaOH Blank 47 031699047 TOX120. 1C9F-G5, 2/22/9 48 031699048 TOX120. 1T0F-G3, 2/22/9 49 031699049 TOX120. .niF-GS, 2/22/99 50 031699050 Toxl20,-RBL02229-250ppb-CC5, 2/22/99 51 031699051 MaOH Blank 52 031699052 53 031699053 54 031699054 55 031699055 56 031699056 * 57 031699057 TOX120. 1T3F-GS, 2/22/9 TOX120. 174F-GS, 2/22/9 TOX120. 17SF-GS, 2/22/9 TOX120, RBL02229-250ppb-CCC, 2/22/99 MaOH Blank TOX120, RBL02229-H2QBlk~2, 2/22/99 58 031699058 TOX120, RBL02229-LivBlk-2, 2/22/9 59 031699059 TOX120, RB102229-5ppb-2, 2/22/99 60 031699060 Toxl20, BBL02229-10ppb-2, 2/22/9' 61 031699061 62 031699062 TOX120, RBL02229-2Sppb-2, 2/22/9 TOX120, RBL02229-S0ppb-2, 2/22/99 63 031699063 TOX120, RBL0222-100ppb-2, 2/22/99 64 031699064 Toxl20, RBL02229-250ppb-2, 2/22/99 65 031699065 Toxl20, RBS02229-S00ppb-2, 2/22/99 66 031699066 67 031699067 TOX120, RBL02229-T30ppb-2, 2/22/99 TOX120, RBL02229-1000ppb-2, 2/22/99 G > i A t oijiahi ' ' tP4 . Analy Rnaly Analy Analy . Stand ' Stand Stand Stand Stand Stand Stand Stand Stand Analy Analy Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC' Analy Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Stand Stand Stand Stand Stand 'Stand Stand Stand Stand Std Cono 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 299.00 299.00 * 299.00 299.00 299.00 299.00 299.00 5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00 Kt 7.878 7.829 7.812 7.828 7.820 7.826 7.830 7.826 7.832 7.830 *7.893 7.849 7.901 7.889 7.850 7.857 7.793 7.857 7.850 * 7.849 7.851 7.827 7.889 7.766 7.770 7.702 7.831 7.775 7.711 7.765 7.761 7.757 7.762 7.763 7.766 7.770 7.759 7.775 7.766 At m 52 93 216 325 751 1966 ' 3677 5126 7909 2007 2569 2200 2150 2715 2299 2120 2652 1786 2773 I960 2185 ' 2065 2303 999 1350 1319 234 175 203 1298 46 99 178 328 730 1710 3403 5157 7163 Response Cone. nag. IDav 51.87 92.97 216.31 325.31 750.60 1965.87 3676.73 5125.94 7909.42 2007.43 2569.44 2199.84 . 2149.83 2714.90 2299.14 2120.11 2651.97 1785.69 `2772.88 5.86 bb 12.91 MM 34.09 MM 52.81 fab 125.83 bb 334.50 bb 628.26 bb 877.10 bb 1355.04 bbX 341.64 MM 438.13 MM 374.67 MM 366.09 Mi 463.11 MM 391.72 bb 360.98 MM 4S2.31 MM 303.56 MM 473.07 MM , .* -2 8 14 -12 5 12 5 -2 13 .. *V *t , i j 31 4Ehki . \||r / 58 1979.57 336.85 bb 13 2184.76 372.08 bb- 24 2064.91 2303.09 998.60 1350.47 1319.16 233.79 175.09 202.65 1298.25 351.50 MM 392.40 bb 168.41 bb jl 228.83 MM 223.45 bb 37.09 bb 27.01 MM 31.74 bb 219.86 bb 18 \ jtja -25 ( ni >/J ]y -26 46.35 98.73 177.97 . 328.19 730.44 1709.98 3402.77 5157.15 7163.23 4.91 MM 13.90 bb 27.51 Ml 53.30 MM 122.37 MM 290.56 MM 581.22 MM 882.46 MM 1226.91 hfaX -18 16 -8 -11 2 -3 -3 -2 2 2 \r* aillI Cal. cjkjf * 3 Ivtrt ouHierj, w U c o W u r C-C ivtrt. 1/0`i!4vk trilcrio. CaIv CkttJci will d a W j in d a W St/i- 4 o VfcnCj uiktllur or n-*! 03l l i f a Ar, ouMifir-J. 3T-P ContVincp/ uiK.A_ WU 3 - t o W , f ? AtnUI W t W j * 4 . 311 Ameli 062496t quantitated and printed on 03/17/99 fay ZAC chetanti. Me * 03/j CD > O 7 / O > \. J Amalla 00491; quantitatad and prlatad on 03/17/99 fey LAC Quantify Calibration ftaport 10X120 Hat Llvar Group 5 and 0 Calibration: a:\rata2.mo\CurwaDB\031C99fa2 Last Modified: ad Mar IT 13:00:30 1890 Printed: Wad Mar 17 15:01:43 1999 ] ] 1 T ] J I ] ! ! Aaalia 062498: quantittad and printed on 03/17/89 fay U C BACK TO MAIN Paga 1 1 ' Quantify Calibration Raport 70X120 Rat Livar Group 8 and t ( Calibration i Os \rata2. VR0\CurvaDR\031C99b2 Laat aodiflod: Wad Mar 17 15:04:50 1999 1 Printad: Wad Mar 17 15:01:48 1999 i l J I J 1 J ] ] :i J BACK TO MAIN 1 Method Report 1 Method file: Last modified: Conpound: Printed: G:\rats2.PRO\HethDB\031699b2 .mdb Wed Mar X7 06:39:09 1999 1: PPHS Wed Mar 17 13:11:35 1999 Coapoond Nu m i Internal Ref.: Quantify Trace: Cone, of Standards: User RP Value: User Peak Factor: Integrate Window Factor: PPHS (Mone] 399.00 > 80.00 Cone. A . 1.000000, Diaablad 1.000000 2.000 1 Retention Time (nine) : Relative Retention Time: Tine Window (mins) : Peak Selectlonr . J Response Type: iHpaiHi 7.343 enabled 0.000, Diaablad 0.620 Nearest Rxtemal Araaa . ' 1 Polynomial Type: Point of Origin: Fit weighting: Axis Transformation: T Units: Peak-to-peak anplifcude: Hau Exclude l/X None 293 Smoothing: Window size (scans): Number of smooths: smoothing method: . Disabled 3 2 Join valley threshold: * Iteduc. peak tailing i Raise baseline maximum: 30.00 70.00 4.00 Draw vartical threahold: 10.00 Detect shoulders: Diaablad . Shoulder threshold: 10.00 J Relative height: Absolute height: 50.00. Enabled 0.00 Disabled Relative area: . 3.00, ftiablad Absolute area: 0.00. Disabled Afful/. ou 2 m BACK TO MAIN >ag. l A C O Ii/ m As Method Report Method fil.i Last modified: Compound: Printed: Q !\rat2.PRO\MthDB\031699b2 .db Mad M*r 17 06: S 09 1511 2: Surr Mod Mir 17 13:11:35 1 5 Compound Mamet Internal Ref.: Quantify Trace: Cone, of Standards: User RF Value: User Peak Factor: Integrate Window Factor: Surr [Kona] TIC rixtd ' 1.000000, Disabled 1.000000 2 .0 0 0 Retention T i m (mins): Relative Retention Time: T i m Window (mins) : Peak Selection: 1 Response Type: . Response: Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation: 8.231, Enabled 0.000, Disabled 0.588 Nearest M aternal Areas Exclude None None | Units: Feak-to-peak anplitude: | [ Smoothing: ` Window else (scans): Number of smooths: Smoothing method: (i i Join valley threshold: * Reduce peak tailing: Raise baseline maxixauu 77 Disabled 3 2 30.00 70.00 4.00 | Draw vertical threshold: 10.00 Detect shoulders: Disabled Shoulder threshold: ,* 10.00 | Relative height: 1 Absolute height: Relative area: . Absolute area: 50.00,, babied . 0.00. Disabled 3.00, babied 0.00, Disabled * . A iJ i& o u m t ___ l A t - BACK TO MAIN Pag. BACK TO MAIN FACT: DATAREVIEWSUMMARYFORM Date: s / s h Analyst: FACTStudy Number ~ 7 ^ k - / g > o Matrix 1 TimePoints Dilutions? 3 Analyte 1: \" j /\J& Pofi rb P f t J S ( S g ~ &>** Acceptable LinearRange: 5 ? LQfor this dataset: ^/>L T Re-evaluatedfix differentrange? Analyte 4: Acceptable LinearRange: LOQfor this dataset: Re-evaluatedfor different range? Anaiyte5: AcceptableLinearRange: LOQfor this data set Ro-evaluatedfbr differentrange? Analyte 2: Acceptable LinearRange: / / - ST r LOQfor ds data set J / Re-evaluatedfor differentrange? _______ J Analyte 3:. ' Acceptable LinearRange: 5~ - ] LOQfarthis dataset Re-evaluated fbr different range? Analyte 6: AcceptableLinearRange: Z LOQfor this dataset: Re-evaluatedfor different, Analyte 7: 7 Acceptable LindarRange: LOQfix this dataset Re-evaluatedfor different range? J' ! BACK TO MAIN Quantify Compound Suaaary Rapert Tox-120 Rat Liv.r ' Saltila Listi H:\rats2.PR0\Saapla0B\04209So Last modified: Thu Apr 22 09:02:11 1999 Mathed: M:\rata2.PRO\MathDB\042099e Last modifiad: Tua May 04 08:S2:4S 1999 Job Coda: Printad: Rad May OS 11:10:51 1999 Compound li POAA # Hu m 1 042099002 2 042099003 3 042099004 4 042099005 5 042099006 6 042099007 7 042099008 8 042099009 9 042099010 10 042099011 11 042099012 12 042099013 13 042099014 14 042099015 15 04209901 1 042099017 17 04209901* 19 042099019 19 042099020 20 042099021 21 042099022 22 042099023 23 042099024 24 042099025 25 042099026 26 042099027 27 042099028 28 042099029 29 042099030 30 042099031 31 042099032 32 042099033 33 042099034 34 042099035 35 042099036 36 042099037 37 042099038 38 042099039 39 042099040 40 042099041 41 042099042 42 042099043 43 042099044 44 042099045 45 042099046 46 042099047 47 042099048 48 042099049 49 042099050 50 042099051 51 042099052 52 042099053 53 042099054 54 042099055 55 042099056 56 042099057 57 042099058 59 042099059 59 042099060 60 042099061 61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 6 042099067 67 042099068 68 042099069 69 042099070 70 042099071 ,71 042099072 72 042099073 73 042099074 74 042099075 75 042099076 76 042099077 77 042099078 78 042099079 79 042099090 80 042099081 81 042099082 82 042099083 83 042099084 84 042099085 85 042099086 86 042099087 87 042099088 88 042099089 Samplallat Taxt MaOH Blank TOX120, RBL0419-H2OBlk-l, 4/15/99 TOX120, RBL04199-LlvBlk-l, 4/19/99 TOX120, RBL04199-Sppb-1. 4/19/99 TOX120, RBL04199-10ppb~l, 4/19/99 TOX120, RBL04199-2Sppb-l, 4/19/99 Toxl20, RBL04199-50ppb-l, 4/19/99 TOX120, RBL04199-100ppb-l, 4/19/99 Toxl20, RBL04199-250ppb-l, 4/19/99 TOX120, RBL04199~500ppb~l, 4/19/99 TOX120, RBL04199-7S0ppb-l, 4/19/99 Toxl20, RBL04199-1000ppb-l, 4/19/99 MaOH Blank TOX120, RTL, 1M, Cl, 4/19/99 TOX120, RTL, 2M, Cl, 4/19/99 TOX120, RTL, 3M, Cl, 4/19/99 TOX120, RTL, 4M, Cl, 4/19/99 TOX120, RTL, 5M, Cl, 4/19/99 TOX120, RBL04199-250ppb-CCl, 4/19/99 MaOH Blank Toxl20, RTL, CM, Cl, 4/19/99 TOX120, RTL, 7M, Cl, 4/19/99 TOX120, RTL, 8M. Cl, 4/19/99 TOX120, RTL, 9M, Gl, 4/19/99 TOX120, RTL, 10M, Cl, 4/19/99 Toxl20, RBL04199-250ppb-CC2, 4/19/99 MaOH Blank TOX120, RTL, 11M, Cl, 4/19/99 TOX120, RTL, U M , Cl, 4/19/99 . Toxl20, RTL, 14M, Cl, 4/19/99 Toxl20, RTL, ISM, Cl, 4/19/99 Toxl20, RBL04199-250ppb-CC3, 4/19/99 MaOH Blank TOX120, RTL, 17M, G2, 4/19/99 TOX120, RTL, 18M, G2, 4/19/99 TOX120, RTL, 19M, G2, 4/19/99 TOX120, RTL, 20M, G2, 4/19/99 Toxl20, RTL, 21M, G2, 4/19/99 TOX120, RBL04199-250ppb-CC4, 4/19/99 MaOH Blank Toxl20, RTL, 22M, G2, 4/19/99 TOX120, RTL, 23M, G2, 4/19/99 TOX120, RTL, 24M, G2, 4/19/99 Toxl20, RTL, 25M, G2, 4/19/99 Toxl20, RTL, 26M, G2, 4/19/99 Toxl20, RBL04199-250ppb-CC5, 4/19/99 MaOH Blank TOX120, RTL, 27M, G2, 4/19/99 Toxl20, RTL, 29M, G2, 4/19/99 TX120, RTL, 30M, G2, 4/19/99' TOX120, RTL, 31M, G2, 4/19/99 Toxl20, RBL04199-250ppb-CC6, 4/19/99 MaOH Blank Toxl20, RTL, 33M, G3, 4/19/99 TOX120/ RTL, 34M, G3, 4/19/99 - Toxl20, RTL, 3SM, Gl, 4/19/99 TOX120, RTL, 36M, G3, 4/19/99 TOX120, RTL, 37M, G3, 4/19/99 TOX120, RBL04199-250ppb-CC7, 4/19/99 MaOH Blank - . ' TOX120, RTL, 38M, G3, 4/19/99 Toxl20, RTL, 39M, G3, 4/19/99 TOX120, RTL, 40M, G3, 4/19/99 ' Toxl20, RTL, 41M, G3, 4/19/99 Toxl20', RTL, 42M, G3, 4/19/99 TOX120, RBL04199-250ppb-CC8, 4/19/99 MaOH Blank Toxl20, RTL, 43M, G3, 4/19/99 Toxl20, RTL, 4SM, G3. 4/19/99 Toxl20, RTL, 46M, G3, 4/19/99 , Toxl20, RTL, 47M, G3, 4/19/99 TOX120, RBL04199-2S0ppb-CC9, 4/19/99 MaOH Blank TOX120, RTL, 5M, G5, 4/19/99 TOX120, RTL, 66M, CS, 4/19/99 Toxl20, RTL, 73M, G5, 4/19/99 ' TOX120, RTL, 74M, C5, 4/19/99 TOX120, RTL. 75M. C5, 4/19/99 Toxl20, RBL04199-250ppb-CC4, 4/19/99 MaOH Blank TOX120, RTL, 77M, C5, 4/19/99 Toxl20, RTL, 78M, GS, 4/19/99 Toxl20, RTL, 79M, CS, 4/19/99 ToxllO, RBL04199-250ppb-CCS, 4/19/99 MaOH Blank Toxl20, RTC, 3M, Gl-2S0ppb-MS, 4/19/99 TOX120, RTL, 3M, Gl-2S0ppb-M3D, 4/19/99 TOX120, RTL, 6M, Gl-2S0ppb-MS, 4/19/99 Std Conn Standard Standard Standard . Standard Standard Standard Standard Standard . Standard 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 QC 278.00 QC 278.00 QC 278.00 QC ' 278.00 QC 278.00 QC 278.00 QC 278.00 QC 271.00 QC 278.00 QC 278.00 QC 278.00 RT 4.483 4.480 4.477 4.479 4.479 4.486 4.484 4.481 4.484 4.483 4.480 4.484 4.480 4.482 4.480 4.471 4.485 4.476 4.479 4.479 4.487 4.485 4.484 4.479 4.484 4.483 4.479 4.479 4.483 4.481 4.479 4.479 4.495 4.453 4.495 4.472 .4.463 4.475 4.488 4.481 4.480 4.483 4.479 4.480 4.486 4.484 4.454 4.459 4.477 4.472 4.485 9aga 1 Am 74 57 77 121 165 374 740 922 1075 Ratpon* Cono. Tlaga %Dv 73.837 57.350 76.531 121.098 165.361 374.157 739.533 921.621 1074.879 32.87 MM 20.70 MM 34.85 MM 67.75 MM 100.42 bb 254.53 bb 524.21 MM 658.60 bbX 771.72 bbX 86 25 22 -10 -8 -6 -21 -31 355 354.908 240.32 bb 447 447.316 308.53 bb 341 341.490 230.42 MM 3899 5143 5371 5320 5764 413 3898.674 5142.572 5371.353 5319.878 5764.340 413.054 2855.93 bb 3774.03 bb 3942.89 bb 3904.90 bb 4232.95 bb 283.24 bb 4423 3922 5424 2795 3068 343 4422.820 3922.056 5424.250 2794.636 3067.762 342.693 3242.79 bb 2873.19 bb 3981.94 bb 2041.05 bb 2242.64 bb 231.30 MM 2119 1448 1545 1644 419 2119.342 1448.030 1544.621 1644.361 418.663 1542.63 bb 1047.14 bb 1118.43 bb 1192.05 bb 287.38 bb 4232 5668 4583 4657 5081 411 4232.225 5668.U9 4582.970 4657.182 5061.245 411.447 3102.12 bb 4161.93 bb 3361.00 bb 3415.77 bb 3728.77 bb 202.05 MM 5631 5631.017 4134.55 m 4587 4517.391 3364.27 Mf 5604 . 5603.629 4114.33 bb 3410 3409.806 2495.10 bb 1319 1318.606 951.61 bb 443 442.641 305.07 bb 2595 1076 ISO 1384 398 2594.520 1075.927 850.322 1383.690 398.107 1893.35 bb 772.50 bb 605.98 bb 999.65 bb 272.20 MM 413 413.360 283.48 MM 364 363.646 246.77 MM 372 372.063 252.98 bb 380 380.105 258.92 MM 385 384.795 262.38 bb .madalina 041099: :quantltatad and printad by maa 5/05/99 i / aadalina 04X099: squantitatad and printad by b m 5/05/99 L -- _i SSSiSS'3SSSSSS5- "H iiiiP i I H 5! - i i l se Pi ;<? 2* 3 5 ! 8 2gK S s|s| ? BSS] SBSS I ili aaaaaat pHHUUaAU(gIn)MsMIhVPUll<NJHMU ssssssssk :sssss3s s H s s 5 H 5 ss3 s s - s s ? Ss s Ssssss s k S S H S s s SS issisi sl=i: SSSSSSSSoS . S38!i2SSSg g|3?SS3m ss-f K2SS? 3?g| i- w^ J t p i i v u o So S a $ BACK TO MAIN Quantify Compound S u n u r y Roport Tox-120 Rat Livor Sampl. Lit: N:\rata2.PRO\SaplaOB\042099a Laflt nodifiad: Thu Ape 23 09:02:11 1999 Mathod: M:\zata2.HU3\MathOB\042099c La.t nodifiad: Tua May 04 09:32:49 1999 Job Coda: Prlntad: Wad May 03 11:10:31 1999 Compound 2: PF0S Mam* Sanplelist Tuet 1 042099002 MeOH Blank . 2 042099003 Toxl20, RBL04199-H20Blk-l, 4/19/99 3 042099004 Toxl20, RBL04199-LivBlk-l, 4/19/99 4 042099005 Toxl20, RBL04199-5ppb-l, 4/19/99 5 042099006 TOX120, RBL04199-10ppb-l, 4/19/99 6 042099007 TOX120, RBL04199-25ppb-l# 4/19/99 7 042099006 TOXI20, RBL04199-50ppb-l, 4/19/99 8 042099009 Toxl20, RSL04199-1OOppb-1# 4/19/99 9 042099010 TOX120, RBL04199-250ppb-l, 4/19/99 10 042099011 TOX120, RBL04199-5OOppb-1, 4/19/99 11 042099012 Toxl20, RBL04199-750ppb-l, 4/19/99 12 042099013 TOX120, RBLQ4199-1000ppb-l, 4/19/99 13 042099014 MeOH Blank 14 042099015 TOX120, RTL,'1H, Cl, 4/19/99 15 042099016 Toxl20, RTL, 2K, Gl, 4/19/99 16 042099017 Toxl20, RTL, 3M, Gl, 4/19/99 17 042099018 TOX120, RTL, 4M, Gl, 4/19/99 18 042099019 TOX120, RTL, SM, Gl, 4/19/99 19 042099020 TOX120, RBL04199-250ppb-CCl, 4/19/99 20 042099021 M*OH Blank 21 042099022 Toxl20, RTL, 6M, Gl, 4/19/99 22 042099023 Toxl20, RTL, 7M, Gl, 4/19/99 23 042099024 TOX120, RTL, 8M, Gl, 4/19/99 24 042099025 Toxl20, RTL, 9H, Gl, 4/19/99 25 042099026 Toxl20, .RTL, ICH, Gl, 4/19/99 26 042099027 Toxl20, RBL04199-250ppb-CC2, 4/19/99 27 042099028 MeOH Blank 28 042099029 Toxl20, RTL, 11M, Gl, 4/19/99 29 042099030 TOX120, RTL, 13M, Gl, 4/19/99 30 042099031 . Toxl20, RTL, 14M, Gl, 4/19/99 31 042099032 Toxl20, RTL, 15M, Gl, 4/19/99 32 042099033 TOX120, RBL04199-250ppb--CC3, 4/19/99 33 042099034 MeOH Blank 34 042099035 Toxl20, RTL, 17M, G2, 4/19/99 35 042099036 Toxi20, RTL, IBM, G2, 4/19/99 36 042099037 Toxl20, RTL, 19M, G2, 4/19/99 37 042099038 Toxl20, RTL, 20M, G2, 4/19/99 38 042099039 Toxl20, RTL, 21M, G2, 4/19/99 39 042099040 Toxl20, RBL04199-250ppb-CC4, 4/19/99 40 042099041 MeOH Blank 41 042099042 Toxl20, RTL. 22M, G2, 4/19/99 42 042099043 . TOX120, RTL, 23M, G2, 4/19/99 43 042099044 Toxi20, RTL. 24M, G2, 4/19/99 44 042099045 Toxl20, RTL. 25M, G2, 4/19/99 45 042099046 TOX120, RTL, 26M, G2, 4/19/99 46 042099047 Toxl20, RBL04199-250ppb-CC5, 4/19/99 47 042099048 MeOH Blank 48 042099049 Toxl20, RTL, 27M, G2, 4/19/99 * 49 042099050 TOX120, RTL, 29M, G2, 4/19/99 50 042099051 Toxl20, RTL, 30M, G2, 4/19/99 51 042099052 . Toxl20, RTL, 3IM, G2, 4/19/99 52 042099053 Toxl20, RBL04199-250ppb-CC6, 4/19/99 53 042099054 MeOH Blank 54 042099055 55 042099056 Toxl20, RTL, 33M, G3, 4/19/99 Toxl20,' RTL, 34M, S3, 4/19/99 56 042099057 Toxl20, RTL, 35M, G3, 4/19/99 57 042099058 Toxl20, RTL, 36M, G3, 4/19/99 58 042099059 Toxl20, RTL, 37M, G3, 4/19/99 59 042099060 Toxl20, RBL04199-2S0ppb-CC7, 4/19/99 60 042099061 MeOH Blank - 61 042099062 TOX120, RTL, 38M, G3, 4/19/99 62 042099063 Toxl20, RTL, 39M, G3, 4/19/99 63 042099064 TOX120, RTL, 4QM.G3, 4/19/99 .64 042099065 TOX120, RTL, 41M, G3, 4/19/99 65 042099066 Toxl20; RTL, 42M, G3, 4/19/99 66 042099067 TOX120, RBL04199-250ppb-CC8, 4/19/99 67 042099068 MeOH Blank 68 042099069 Toxl20, RTL, 43M, G3, 4/19/99 69 042099070 Toxl20, RTL, 45M, G3, 4/19/99 70 042099071 71 042099072 Toxl20, RTL, 46M, G3, 4/19/99 T0XI20, RTL, 47M, 63, 4/19/99 72 042099073 ' Toxl20, RBL04199-250ppb-CC9, 4/19/99 73 042099074 MeOH Blank 74 042099075 TOX120, RTL, 65M, GS, 4/19/99 75 042099076 Toxl20, RTL, 66M, GS, 4/19/99 76 042099077 TOX120, RTL, 73M, GS, 4/19/99 77 042099078 TOX120, RTL, 74M, G5, 4/19/99 78 042099079 Toxl20, RTL. 75M, G3, 4/19/99 79 042099080 Toxl20, RBL04199-250ppb-CC4, 4/19/99 80 042099081 MeOH Blank . 81 042099082 Toxi20, RTL, 77H, G5, 4/19/99 82 042099083 Toxl20, RTL, 78M, 65, 4/19/99 83 042099084 OX120, RTL, 79M, G5, 4/19/99 84 042099085 TOX120, RBL04199-250ppb-CC5, 4/19/99 85 042099086 * MeOH Blank 86 042099087 Toxi20, RTL, 3M, Gi-250ppb-MS, 4/19/99 87 042099088 Toxi20, RTL, 3M, Gl-250ppb-KS0, 4/19/99 88 042099089 Toxl20, RTL, 6M, Gl-250ppb-MS, 4/19/99 typa Std Cono Standard Standard Standard Standard Standard Standard Standard Standard Standard 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 QC 278.00 OC 278.00 QC 278.00 QC 278.00 QC 278.00 QC 278.00 QC 278.00 QC 278.00 QC 278.00 QC 278.00 QC 278.00 RT 4.822 4.823 4.819 4.816 4.819 4.818 4.825 4.823 4.820 4.779 4.822 4.818 4.824 4.817 4.780 4.822 4.824 4.82t) 4.823 4.823 4.821 4.820 4.823 4.824 4.824 4.821 4.824 4.819 4.821 4.819 4.810 4.779 4.813 4.819 4.819 4.827 4.824 4.778 4.818 4.823 4.822 4.818 4.819 4.823 4.820 4,819 4,818 4.787 4.789 4.788 4,811 4.803 4.813 4.782 4.820 4,819 4.823 4.818 4.820 4.825 4.823 4.824 4.829 4,780 4.790 4.804 4,791 . 4.810 4.816 4.773 4.795 4.792 4.816 4.811 4.781 Area Raapon. Cono. Flage tDav 114 142 182 310 510 835 1972 3926 5290 * 6382 113.879 142.359 181.B4S 310.291 510.134 834.975 1972.331 3925.785 5290.446 6342.225 0.00 bb 3.38 bb 8.76 bb 26.24 bb 53.43 bb 97.63 bb 252.40 bb 518.22 MM . 703.92 MMX 852.48 bbX -39 -2-16 -4 -12 -9 -7 -IS -23 1409 1067 863 1032 874 2082 1409.125 1086.545 ' 863.333 1032.293 674.073 2081.914 175.7 bb 129.14 bb 101.49 bb 124.48 bb 102.95 MM 267.31 bb 1007 1047 1342 956 2596 2257 1007.439 1046.567 1341.526 956.335 2595.882 2256.951 121.10 bb 126.43 bb 166.56 bb 114.15 bb 337.25 bb 291.13 bb `2384 804 907 813 1926 2363.945 604.193 906.706 813.172 1926.462 308.41 bb 93.44 bb 107.39 bb 94.67 bb 246.16 bb 514 413 411 383 371 2309 514.195 412.578 411.485 343.146 370.594 2309.480 53.98 bb 40.16 bb 40.01 bb 36.15 bb 34.44 bb 298.28 bb 309 259 330 360 307 2006 309.189 259.486 330.015 360.292 307.436 2006.424 26.09 bb 19.32 bb 28.92 bb 33.04 bb 25.85 bb 257.04 bb 304 444 810 349 2312 304.184 444.249 810.409 346.586 2312.079 25.41 bb 44.46 bb 94.29 bb 31.45 bb ' . 298.63 bb 642 . 642.037 . 71.38 bb 4B1 484.537 50.22 bb 587 596.903 63.88 bb 545 544.943 58.17 bb 669 669.401 75.10 bb 2265 2264.934 292.22 bb 472 4 472.107 48.26 bb 713* x 713.120 61.05 bb 60S 604.755 93.52 bb 421 421.200 *41.33 bb 588 587.601 63.97 bb 2329 2328.665 300.89 bb 487 559 509 530 2293 466.624 558.781 509.226 529.702 2293.406 50.23 bb 60.05 bb 53.31 bb 56.09 bb 296.09 bb . 328 660 1251 825 781 2331 326.225 660.033 1251.132 125.343 780.944 2330.889 28.68 MM 73.83 bb 154.26 bb 96.32 bb 90.28 bb 301.19 bb 974 767 730 2166 50 2869 2955 3061 973.805 767.281 729.592 2166.195 50.002 2669.270 2954.571 3060.699 116.52 bb 88.42 bb 83.29 bb 278.78 bb 0.00 bb 374.45 bb 386.06 bb 400.50 bb Pag* 3 madallna 041099: :quantitatad and prlntad by aaa 3/03/99 ss o* < C J' slSS n m r* Owho QOAHO Ar h nei n fi SSSSSS oNr ootnoc ONWHV hf M WoOFIfA'ONrMt SNbb oNa nNA anN iNnhn.OA__M_HH_P_o)_Ha_Uo_I_w0_trh_M1 (UnrAntnrAtrF(MithtnHnBnnoNW<wArVnHv4hnO) |jfbA*oAwnAnan fm*noHtirotArintwnn!wmmonnrm*nooviiimnoh NWN H N V P* S NN iFA'*rO> rt NTOrBtOhf frltArOtVorAArtO MOrOt BOWOQOOOOOOOO fiHHNMHniHHHnNFWfAfl BninHH TJTITJTJTJTJTITJTI liim ifl I nnnnnnnnu NAH*HAS*SH^OASPHASI I mANASHPoASSAPHiAAAHSAHSASA_ ASASrAtSSAHAAAPHANASAtHVANAAPHASSAAPHSASA,rABNAlOSl 'I3.9Nt . j#2 i;i; Sn S" 8 *8 * I ils* si 1 3U hU! aI vH vH iO WnHnHlAH _1LSs -s4 i0.l0 AaOiOsf-l_O_Of_O_l_OO JflHNiflHNflFrt JAritAJritAritAmHArAtAHirAAtHAmrAtArAtirAJti ^s|sssassassss gioNHoNUpHHNpHHoXHNopHOX^3oHHXNoXpHNiUoHprNHooXtNHpoHKNHHooXMNHooHNXHooNHoo4XNHpHoUN'ooirXtMHooH H l i mOrAtAN FAIAa fblmU hA oooooooo AAONAAON AAON AAON AOAN AOAN AOAN AOAN ooamoenOmoN*oaoNief i%mOl oi Ol >4oMoN o o o o o o o o o o o o oot Ho Nof< J ) f l madlin 041099::quantitat*d and printed by m m 5/05/99 i IPff |. 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Lait Bsdlfiad! Thu Apr 22 09:02:11 1999 Mathod: M:\rata2.PR0\MathOB\042099o Laat aodlflad: Tua Hay 04 08:S2:4B 1999 Job Coda: ' ] Brintad: ad May OS 11:10:51 1999 ; ] ] Conpound 3: FTHS i Rana 89 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 9$ 042099097 97 042099098 98 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105 Sanplali.t Taat * TOX120, RTL, H, 61-2S0ppb-M3D, 4/19/99 8td Cono TOX120, RTL. 13M, Gl-2S0ppb-MS, 4/19/99 TOX120, RTL, 13M, Gl-250ppb-MSD, 4/19/99 TOX120, RBL04199-250ppb-CC6, 4/19/99 QC 278.00 MaOH Blank TOX120, RBL04199-H2OBlk-l. 4/19/99 TOX120, RBZ.04199-LivBlk-l, 4/19/99 . Toxl20, RBL04199-Sppb-1, 4/19/99 Standard 5.56 Toxl20, RBL04199-10ppb-l, 4/19/99 - Standard 12.10 Toxl20, RSL04199-2Sppb-l, 4/19/99 Standard 27.80 Toxl20, RBL04199-50ppb-l, .4/19/99 Standard , 55.60 TOX120, RBL04199-100ppb-l, 4/19/99 Standard 111.00 TOX120, RBL04199-2S0ppb-l, 4/19/99 Standard 278.00 TOX120, RSL04199-S00ppb-1> 4/19/99 Standard 556.00 TOX120, RBL04199-7S0ppb-l, 4/19/99 Standard 833.00 TOX120, RBL04199-1000ppb-i;- 4/19/99 Standard 1111.00. . RT 4.115 4.117 4.117 4.109 4.117 4.161 4.162 4.112 4.117 4.116 4.116 4.119 4.103 4.117 4.116 4.119 BACK TO MAIN ' , ___ Paga ( - .. . ' At m 6240 7204 7649 5035 53 49 43 126 237 597 1283 2466 5941 11407 16687 18582 Raapons 6240.264 7203.939 7648.546 5035.472 52.570 49.377 42.961 126.050 . 237.146 596.977 1282.536 2465.863 5941.001 11406.754 16686.936 18581.658 Cono. Flags %Dv . 321.94 bb 371.71 bb 394.66 bb \ ) j C a hrul 259.74 MM -7 2.45 MM 2.29 MM 1.96 MM 6.25 MM 12 11.98 bb 8 30.56 bb 10 65.96 bb 19 127.06 MM 14 306.50 bb 10 588.71 bb 6 861.35 bb 3 959.18 bbX -14 * I1 j 1 I ] > j J ! I mad*lina 041099: :quanti tt*d arid print* by mmm 5/05/99 BACK TO MAIN 1 -- - i --------------------- - _____________ ___________________________________ r:--------------------------------------------- Quantify Compound Sumnary fUport Tox-120 Rat Livor ~ * Pag* ? Sampla Lift: H:\nts2.PftO\StmplsOB\042099c Last modified: Thu Apr 22 09:02:11 1999 Mothod: N:\rats2.PRO\MathDB\042099a Last modiflad: Tua May 04 08:52:48 1999 Job Coda: Printad: Wad May 05 11:10:51 1999 1 J 1 I j J 3 ! Compound 4: ZS Mama 1 042099002 2 042099003 3 042099004 4 042099005 5 042099006 042099007 7 042099008 8 042099009 9 042099010 10 042099011 11 042099012 12 042099013 13 042099014 14 042099015 15 042099016 16 042099017 17 042099018 18 042099019 19 042099020 20 042099021 21 042099022 22 042099023 23 042099024 24 042099025 25 042099026 26 042099027 27 042099028 28 042099029 29 042099030 30 042099031 31 042099032 32 042099033 33 042099034 34 042099035 35 042099036 36 042099037 37 042099038 38 042099039 39 042099040 40 042099041 41 042099042 42 042099043 43 042099044 44 042099045 45 042099046 46 042099047 47 042099048 48 042099049 49 042099050 50 042099051 51 042099052 52 042099053 53 042099054 54 042099055 55 0 4 2 0 9 9 0 5 6 56 042099057. 57 04209905B 58 042099059 59 042099060 60 042099061 61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 66 042099067 67 042099068 68 042099069 69 042099070 70 042099071 71 042099072 72 042099073 73 042099074 74 042099075 75 042099076 76 042099077 77 042099078 78 042099079 79 042099080 80 042099081 81 042099082 82 042099083 83 042099084 84 042099085 85 042099086 86 042099087 87 042099088 88 042099089 Sainplaliat Text M.0H Blank TOX120, RBL04199-H20BU-1, 4/19/99 IOX120, RBL04199-LivBlk-l, 4/19/99 Toxl20. R8L04199>5ppb-l, 4/19/99 Toxl20, RBL04199-10ppb-l, 4/19/99 TOX120, BBL04199-2Sppb-l. 4/19/99 Toxl20. RBL04199-S0ppb-1, 4/19/99 TOX120. RBL04199-100ppb-l, 4/19/99 TOX120, RBLO4199-2S0ppb-l, 4/19/99 TOX120, RBL04199-500ppb-l, 4/19/99 Toxl20. RBL04199-7S0ppb-l, 4/19/99 TOX120, RBL04199-1000ppb-l, 4/19/99 HaOH Blank TOX120, RTL, IN, Gl, 4/19/99 TOX120, RTL, 2M, Gl, 4/19/99 TOX120, RTL, 3M, Gl, 4/19/99 TOX120, RTL. 4M, Gl, 4/19/99 TOX120, RTL, SN, Gl, 4/19/99 . TOX120, RBL04199-250ppb-CCl, 4/19/99 HaOH Blank TOX120, RTL, CM, Gl. 4/19/99 TOX120. RTL, 7H, Gl, 4/19/99 Toxl20, RTL, 8M, Gl, 4/19/99 TOX120, RTL. 9M, Gl, 4/19/99 TOX120, RTL, 10H, Gl, 4/19/99 TOX120, RBLO4199-2S0ppb-CC2, 4/19/99 HaOH Blank TOX120, RTL, 11M, Gl, 4/19/99 TOX120, RTL, 13M, Gl, 4/19/99 TOX120, RTL, 14H, Gl, 4/19/99 . * TOX120, RTL, ISM, Gl, 4/19/99 TOX120, RBL04199-250ppb-CC3, 4/19/99 HaOH Blank TOX120, RTL, 17N, G2, 4/19/99 TOX120, RTL, ISM, G2, 4/19/99 TOX120, RTL, 19M, G2, 4/19/99 TOX120, RTL, 20H, G2, 4/19/99 TOX120, XTL, 21M, G2, 4/19/99 TOX120, RBL04199-250ppb-CC4, 4/19/99 MeOH Blank Toxl20, RTL, 22M, G2, 4/19/B9 Toxl20, RTL, 23M, G2, 4/19/99 ' TOX120, RTL. 24M, G2, 4/19/99 TOX120, RTL, 25M, G2, 4/19/99 . Toxl20, RTL, 26M, G2, 4/19/99 TOX120, RBL04199-2S0ppb-CCS, 4/19/99 HaOH Blank Toxl20, RTL, 27M, G2, 4/19/99' TOX120, RTL, 29M, G2, 4/19/99 TOX120, RTL, 30M, G2, 4/19/99 TOX120, RTL, 3IN, G2, 4/19/99 TOX120, RBL04199-2S0ppb-CCC, 4/19/99 HaOH Blank TOX120, RTL, 33H..G3, 4/19/B9 TOX120, RTL. 34M, G3, 4/19/99 Toxl20, RTL. 35M, G3, 4/19/99 TOX120, RTL, 3M, G3, 4/19/99 TOX120, RTL, STM, G3, 4/19/99 TOXI20, RBL04199-250ppb-CC7, 4/19/99 HaOH Blank . . TOX120, RTL, 38H, G3, 4/19/99 TOX120, RTL, 39M, G3, 4/19/99 . TOX120, RTL, 4OH, G3, 4/19/99 TOX120, RTL, 4IN, G3, 4/19/99 Toxl20,.RTL, 42M, 63, 4/19/99 ' TOX120, RBL04199~230ppb-CCI, 4/19/99 HaOH Blank TOX120, RTL, 43H, G3, 4/19/99 TOX120, RTL, 4SM, G3, 4/19/99 TOX120, RTL, 4CM, G3, 4/19/99 TOX120, RTL. 4TM, G3, 4/19/99 TOX120, RBL04199-250ppb-CC9, 4/19/99 HaOH Blank Toxl20, RTL,. C5M, GS, 4/19/99 Toxl20, RTL, 6CM, GS, 4/19/99 TOX120, RTL, 73M, GS, 4/19/99 TOX120, RTL, 74M. G5, 4/19/99 TOX120, RTL, 75M, GS, 4/19/99 TOX120, RBL04199'2SOppb-CC4, 4/19/99 HaOH Blank TOX120, RTL, 77M, GS, 4/19/99 TOX120, RTL, 78M, G5, 4/19/99 TOX120, RTL, 79M, G5, 4/19/99 TOX120, RBL04199'2S0ppb-CCS, 4/19/99 HaOH Blank TOX120, RTJ|, 3M, Gl-2S0ppb-MS, 4/19/99 TOX120, RTL, 3H, Gl-25Oppb-MS0, 4/19/99 TOX120, RTL, 6M. Gl-250ppb-MS, 4/19/99 *n Std Cono Standard Standard Standard Standard Standard Standard Standard Standard , Standard 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 OC ' 1.00 OC 1.00 . oc 1.00 oc - 1.00 oc 1.00 oc 1.00 oc 1.00 oc 1.00 oc 1.00 oc 1.00 . oc 1.00 M ATM Mssponsm Cono, Flsgs %0v 4.479 4.477 4.477 4.475 4.472 4.474 4.473 4.481 4.478 4.475 4.479 11168 14262 17108 14220 14163 15047 14165 12687 13127 12184 14980 11168.010 14262.124 17107.816 14220.119 14162.521 15047.055 14164.800 12686.997 13127.305 12183.721 14960.457 0.72 bb 0.92 bb 1.10 bb 0.92 bb 0.91 bb 0.97 bb 0.91 bb 0.82 bb 0.85 bb 0.79 bb 0.97 MK -28 -8 10 -8 -9 -3 -9 -18 -15 -21 -3 4.477 4.473 4.479 4.473 4.480 4.477 13354 15480 16743 16108 16428 12965 13353.618 15479.964 16743.496 16107.631 16427.918 12964.758 0.86 bb 1.00 bb 1.08 bb 1.04 bb 1.06 bb 0.84 bb -14 0 8 4 6 -16 4.479 4.476 4.478 4.478 4.476 4.475 12592 12908 14767 13113 . 16767 15329 12591.617 12908.252 14767.411 13113.133 16766.613 15328.990 0.81 bb 0.83 bb 0.95 bb . 0.85 bb 1.08 bb 0.99 bb -19 -17 -5 -15 8 -1 4.476 4.479 4.479 4.476 4.479 .14482 11815 13252 12072 12820 14482.320 11815.149 13252.295 12071.706 12819.677 0.93 bb 0.76 bb 0.86 bb 0.78 bb 0.83 bb -7 -24 -14 -22 -17 4.474 4.476 4.475 4.465 4.434 4.471 7188 6839 6975 7305 7576 15008 7188.418 6839.444 6975.442 7304.985 7576.205 15007.997 0.46 bb 0.44 bb 0.45 bb 0.47 bb 0.49 bb 0.97 bb -54 -56 -55 -53 -51 -3 4.473 4.474 4.438 4.434 4.434 4.473 7420 8272 8265 9236 9268 14716 7419.602 8272.232 8264.774 9235.608 9268.021 14716.280 0.48 bb 0.53 bb 0.53 bb 0.60 MM 0.60 bb 0.95 bb -52 -47 -47 -40 -40 -5 4.478 4.434 4.473 4.429 4.478 8715 12268 10776 11197 14400 8714.930 12268.174 10776.486 11196.844 14399.545 0.56 bb 0.79 bb 0.70 bb. 0.72 bb . 0.93 bb -44 -21 -30 -28 -7 4.475 4.474 4.430 4.445 4.448 4.445 16537 8276 7717 9574 7747 15240 16537.041 274.272 7716.823 9574.318 7747.233 15240.495 1.07 bb 0.53 bb 0.50 bb 0.62 bb 0.50 bb 0.98 bb 7 -47 -50 -38 -50 -2 4.467 4.456 4.426 4.437 4.475 4.475 7589 7588.534 9947* 9947.499 7538 7537.861 8481 ' 8480.613 12183 12182.729 ,14952 14951.948 0.49 bb 0.64 bb 0.49 bb * 0.55 bb 0.79 bb 0.97 bb -51 -36 -51 -45 -21 -3 4.434 4.474 4.475 4.435 4.478 8922 9854 13112 9645 15910 8921.938 9854.359 13111.587 . 9645.204 15909.816 0.58 MM 0.64 bb 0.85 bb 0.62 bb 1.03 bb -42 -36 -15 -38 3 4.478 ^' 10254 4.475 11430 4.434 14854 4.442 14617 4.461 14512 4.449 15752 10253.570 11430.374 14853.702 14617.353 14511.814 15752.205 0.66 bb 0.74 bb 0.96 MM 0.94 bb 0.94 bb 1.02 bb -34 -2 -4 -6 . -6 2 4.465 4.473 4.428 4.453 26825 16234 16282 15674 26824.668 16234.444 16281.634 15674.282 1.73 bb 1.05 MM 1.05 MM 1.01 bb 73 5 5 1 4.472 4.466 4.480 15718 17154 1S585 15717.900 17154.322 15585.048 1.01 bb 1.11 bb 1.01 bb 1 11 1 madalina 041099::quantitatad and printad by maa 5/05/99 Quantify Compound Summary Raport Tox-120 Rat Livar Sampla Liat: H:\rata2.PRO\3mqplmOB\042099a Last modifiedr Thu Apr 22 09:02:11 1999 Mathod: K:\rata2.PRO\MathOB\042099e Last modiflad: Tua Hay 04 0:S2:48 1999 Job Coda: Printad: Wad Hay OS 11:10:S1 1999 Compound 4: XS Mama 89 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 04209909S 95 042099096 96 042099097 97 042099098 98 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105 Saxplaliat fast Typ Std-Cono TOX120, RTL, CM, Gl-250ppb-H3D, 4/19/99 Toxl20, RTL, 13M, Gl-250ppb-MS, 4/19/99 TOX120, RTL, 13M, Gl-2S0ppb-MSD, 4/19/99 10x120, RBL04199-250ppb-CC4, 4/19/99 oc 1.00 MeOH Blink TOX120, RBL04199-H2OBlk>l, 4/19/99 TOX120, RBL04199-Llv81k-1, 4/19/99 TOX120, RBL04199-Sppb-1, 4/19/99 TOX120, RBL04199-10ppb-l, 4/19/99 . Toxl20, RBL04199-2Sppb-l, 4/19/99 TOX120, RBL04199-S0ppb-1. 4/19/99 Toxl20, RBL04199-100ppb-l, 4/19/99 TOX120, RBL04199-2S0ppb-l, 4/19/99 TOX120, RBL04X99-S00ppb>l, 4/19/99 TOX120, RBL04199-7S0ppb~l, 4/19/99 TOX120, RBL04199-I000ppl>-1,- 4/19/99 Standard Standard Standard Standard , Standard Standard Standard Standard Standard 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 RT 4.477 4.478 4.435 4.470 4.478 4.478 4.473 4.435 4.433 4.434 4.433 4.465 4.434 4.434 4.435 Paga B Araa 15039 14583 15562 16368 13605 17110 20728 17142 16514 17079 17088 15845 15402 15262 16108 Rasponsa 15038.934 14582.552 15562.266 16367.936 13604.745 17109.813 20728.434 17141.998 16513.951 17078.783 17087.787 15844.741 15402.126 15261.598 16108.356 Cone, flag %Dav 0.97 bb -3 0.94 bb -6 1.00 bb 0 1.06 bb 6 0.88 bb 1.10 bb 1.34 bb 1.11 bb 1.07 bb 1.10 bb 1.10 bb 1.02 bb 0.99 bb 0.99 MM 1 *04 bb -12 10 34 11 7 10 10 2 -1 4 : . madalina 041099: :quanti tatad and printad by maa S/OS/99 Quantify Calibration flaport Tox-120 Rat Livar Calibration: N:\rata2.FRO\CurvaOB\042099o Last nodifiad: Rad May 05 09:00:19 1999 Frintad: Wad May 05 11:11:10 1999 'Compound 1 name: POAA : Coefficient of Determination: 0.989036 BACK TO MAIN Paga 1 mdalin* 04 1099m quantit tad and prlntad by m-- 5/05/99 1 ________ Quantify Calibration Raport To*-120 Rat Livar I Calibration: M:\rats2.PRO\CurvaDB\042099o Last Modifiad: Had Hay OS 09:00:19 1999 Printad: Wad Hay 05 11:11:10 1999 j i ] ] 1 1 J 1 3 j I BACK TO MAIN BACK TO MAIN . Quantify Calibration Raport Tor-120 ]Ut U n c "* . ` p9 3 Calibration: N:\rati2.FWO\CurvaDB\042099c Laat aodifiad: Wad May 03 09:00:19 1999 Frintad: Wad May 03 11:11:10 1999 Quantify Calibration lUport Tox-120 Sat 1*1var Calibration: H:\rati2.mo\CurvaD8\042099a baat nodifiad: Wad Ky 05 09:00:19 1999 Printad: Wad May 05 11:11:10 1999 - * ' BACK TO MAIN W9a 4 - adallna 041099 :quantittad and. printad by aaa 5/05/99 Method Report Method file: Last modified: Compound: : * Printed: N:\rats2. FRO\MthOB\042099c.adb Tut Hay 04 08:02:41 1999C 1: P O M Mad May S 11:10:26 1999 Compound Name: Internal R.f.i Quantify Trace: Cone, of Standards: User RF Value: User Peak Factor: Integrate Window Factor: Peak Location Retention Time (mins): Relative Retention Tine: Time Window (mins): Peak Selection: Response Response Type: Response: Calibration Curves Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation: Concentration Units: Noise Peak-to-peak ampintude: Smooth Smoothing: Window size (scans): Number of smooths: Smoothing method: Baselines Join valley threshold: Reduce peak tailing: Raise baseline maximum: Peak Separation Draw vertical thraahold: Detect shoulders: Shoulder threshold: : % Response Threshold Relative height: Absolute height: Relative area: Abaolutt araa: POAA {None] TIC Cone. A 1.000000, Disabled 1.000000 2 .0 0 0 4.400, Enabled 0.000, Disabled 0.B34 Nearest External Areas Linear Exclude 1/X None 434 Disabled '3 2 20.00 50.00 5.00 10.00 Disabled 40.00 1.50, Disabled 0.00, Disabled 2.00, Disabled 0.00, Dlsabltd . ' . BACK TO MAIN Pag. l H ouU k u omirtE f IAC, O Id I9 * : H.thod Report Method file: Last modified: Compound: Printed: N:\rats2.PRO\HethDB\042099c.mdb Tue Hay 04 04:52:4 19990 2: PFOS Ned Hay 05 11:10:26 1999 Compound Name: Internal Ref.: Quantify Trac: Cone, of Standards: pros [None] TIC Cone. A . * Usar Rr Valu: Usar Peak Tactor: Intagrat Window Tactor: 1.000000, Disabled 1.000000 2.000 Paak Location ] Retention Tina Inina): Relative Retention Tine: Tina Window Inins): Peak Selection: 4.780, Enabled 0.000, Disabled 0.834 Nearest Response ] Response Type: Response: Calibration Curves External Areas . 1 * Polynomial Type: Point of Origin: Tit Weighting: Axis Transformation: Linear Exclude i/X None 1 Concentration Units: Noise Peak-to-peak amplitude: Smooth 434 Smoothing: Window site (scans): Number of smooths: Smoothing method: Disabled 3 2 Baselines Join valley threshold: 20.00 Reduce peak tailing: 50.00 Raiaa baaalina maximum: - , 3.00 Peak Separation Draw vertical thraahold: Detect ahould.rx; Shoulder thraahold: t 10.00 Disabled 40.00 J Response Threshold Relative height: Absolute height: Relative area: Absolute area: 1.50, Disabled 0.00, Disabled * 2.00, Disabled 0.00, Disabled ] I i J ! - " BACK TO MAIN - .. P9. 2 M a W CSLcM I C nL Mathod Report Method file: Last modified: Compound: Printad: N:\rats2!.PRO\MethDB\042099c.ndb Tue May 04 08:52:48 19990 3: PFKS Wed May 05 11:10:2 1999 ' Compound Name: Internal Ref.! Quantify Trace: Cone', of Standards: User RF Value: User Peak Factor: Integrate Window Factor: Peak Location Rcttntion Time (mins): Relative Retention Time: Time Window (mins): Peak Selection: Response Response Type: Response: . Calibration Curves Polynomial Typo: Point of Origin: Fit Weighting: Axis Transformation: Concentration Units: Noise Peak-to-peak amplitude: Smooth Smoothing: Window size (scans): Number of smooths: Smoothing method: . Baselines Join valley threshold: Reduce peak tailing: Raise baseline maximum: Peak Separation Draw vertical threshold:. Detect shoulders: Shoulder threshold: t Response Threshold Relative height: Absolute height: Relative area: Absolute areal PFH5 (NoneJ TIC Cone. A ' 1.000000, Disabled 1.000000 2.000 . 4.120 Enabled 0.000 Disabled 0.834 Nearest . External Areas Linear Exclude 1/X None 434 Disabled 3 2 20.00 50.00 5.00 10.00 Disabled 40.00 1.50, Disabled 0.00, Disabled 2.00, Disabled 0.00, Disabled BACK TO MAIN Pege 2 M a c U i m , HlDtf LAC/ r /w f/tt Method Report Method file: Last modified: Compound: Frinttd: N:\rata2.PRO\MathDB\042099c.Bdb Tu M a y 04 04:52:48 19990 4: IS Wed M ay 05 11:10:2 1999 Compound Nam*: Internal Ref.: Quantify Trace: Cond. of Standard: , IS (None] TIC Fixed User RF Value: User Peak Factor: Integrate Window Factor: Peak Location 1.000000, Disabled 1.000000 2 .0 0 0 Retention Time (mins): Relative Retention Time: Time Window (mins): Peak Selection: Response 4.490, Enabled 0.000, Disabled 0.034 Nearest Response Type: Response: Calibration Curves . External Areas . Polynomial Type: Point of origin: Fit Weighting: Axis Transformation: Concentration Linear Exclude 1/X None Units: Noise Peak-to-peak amplitude: Smooth 434 Smoothing: Window size (scans): Number of smooths: Smoothing method: Disabled 3 2 Baselines Join valley threshold: Reduce peak tailing: Raise baseline maximum; Peak Separation 20.00 50.00 5.00 Draw vertical threshold: Detect shoulders: Shoulder threshold: , Response Threshold 10.00 Disabled 40.00 Relative height: Absolute height: Relative area: Absolute area: 1.50, Disabled 0.00, Disabled J 2.00, Disabled 0.00, Diaabled . BACK TO MAIN . Fag 4 (WIDM ___HcuUiki I .. 1 AC. QSltX1m ii BACK TO MAIN // AMELIA062498: FACT Equipment Log 99016 QuattroII,#6448E PAGE DATE: 04/20/99 . ANALYST: mee STUDY NUMBER Tox-120 MATTOX: Rat Liver DESCRIPTION: ip FACT Method: ETS-8-5.0 Injectionvolume,10uL Cycletime: 9.0min. Electrode:Z-Spray MobilePhase: (A )2.0m M NH4CH3CQ2 (B)MeOH TN-A- orstndrdnumber (A) 99012-5-22 IB)TN-A-2414 Prepared(date/by): (A)4/16/99/ok (B)4/9/99_hoj_ Expires: (A) 10/99 (B) 10/99 Storeatroomtemp? [Y] or N Other__ Guard column: None ___________ Partnumber na Lot number na Analyticalcolumn:keystonebetasil________________________ Size:2x 50mm____________ _ Type:_cl8________ Spheresize:5 um Partnumber 055-701-2 Lotnumber P7L04 Serialnumber.0481172z Analyticalparameters: i Dryinggas(L/hr):300 . ESInebulizinggas(L/hr)15 LC Flowrate:300uL/min. Backpressure:59bar Sheathgas(L/hr)_0 ^ HPLC solventgradient:long2 Time (min.) 1) 0.00______ 2) 4.5_______ 3 6.5______ 5) 7.0______ %B 40 95 95 40_ : ' - ' Tuning Method Report " .. * " Method: C :\MASSLYNX\RATS2. PRO\ACQUDB\PFOS Printed: Tue Apr 20 17:06:38 1999 1 " 11 7 / ^ - BACK TO MAIN /^tT _. .... r '. PagQ 1 SOURCE ( ESP- ) Capillary Cone Extractor _ RF Lens Source Block Temp. Desolvation Temp. Set Rdbk 3.00 15 5 0.2 150 250 2.99 -14 -6 152 258 Pressures . Analyser Vacuum tas Cell Rdbk 1.6e-4 4.7e-3 MSI LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 . Multiplier Set Rdbk - 15.0 15.0 2.0$ 0.0 57 650 MS2 LM Resolution HM Resolution Collision . Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier Set Rd 15.0 15.0 30 2.0 0.0 40 0 650 32 39 30 FACT:DATAREVIEWSUMMARYFORM BACK TO MAIN Date: 4iW-IW ] j 7 J a j ^ usjua . ^( / U L - <9{p H frb& j ^ Srr - b uJr cwJ*< U /^/s$ . AaaXa 062498: teviawad and quantitatad by kjh B iiS llB w B iliii Illumini *ii il ii ii ii i^ ^ r i P ^ P ^ i F P P P . ^ ii ii ii ii i- o - o m$ mm mm i n a i h i n ? ;i' ill ' ' f ill ' IsSSSSKSo. sssssssss s .S ss S IsIsIsBSo. O s sssssssss / EssIssBississiB^sss^SsSSssssISssSs^sssHssssis slb5itSassbs:s*wisoko'gS'22iskssskStibssgisai5sssss2ssaSk sS:b5bbt:is5k3sb3isSssK:ss:th: oii.kiss?? $$$SiS$$SS&&&gS&5&&SS91HB$SgSSiSB2 .m" m o . o.SKSKtJSo. I V-- y ---- U 7 % Quantify C alibration Aaport C a lib ra tio n : C:\KMSUnoc\rata2.mo\CurvaDB\04209M L aat m o dlflad: Thu Apr 22 07:30:53 1 9 * 9 F rin ta d : Thu Apr 22 07:31:SS 1*99 BACK TO MAIN Amalia 092499: Raviawad and quantittad by kjh 4/22/99 Masslynx - Sample List Sample List: C:\M ASSLYNX\rats2.PRO\Sam pleDB\042099c.SPL Printed: Tue Apr 20 16:51:50 1999 - i/ B A C K T O M A IN /r~ctr , Pagel '. File Nam e File Text 1 042099002 MeOH Blank 2 042099003 Tox120, R B L04199-H 20B lk-1,4/19/99 3 042099004 Tox120, R B L04199-LivB lk-1,4 /19/99 4 042099005 Tox120, RBL04199-5ppb-1,4 /1 9 /9 9 5 042099006 Tox120, R B L04199-10ppb-1.4 /19/99 6 042099007 Tox120, RBL04199-25ppb-1,4 /1 9 /9 9 7 042099008 Tox120, RBL04199-50ppb-1,4 /1 9 /9 9 8 042099009 Tox120, RBL04199-100ppb-1, 4/19/99 9 042099010 Tox120, RBL04199-250ppb-1,4 /1 9 /9 9 10 042099011 Tox120, RBL04199-500ppb-1,4 /1 9 /9 9 11 042099012 Tox120, RBL04199-750ppb-1,4 /1 9 /9 9 12 042099013 Tox120, RBL04199-1000ppb-1,4 /1 9 /9 9 13 042099014 MeOH Blank 14 042099015 Tox120, RTL, 1M, G 1 .4/19/99 15 042099016 TOX120, RTL, 2M , G 1.4 /1 9 /9 9 16 042099017 Tox120, RTL, 3M, G 1 ,4/19/99 17 042099018 Tox120, RTL, 4M . G 1 ,4/19/99 18 042099019 Tox120, RTL, 5M, G 1 ,4/19/99 19 042099020 Tox120, RBL04199-250ppb-CC1,4 /1 9 /9 9 20 042099021 MeOH Blank 21 042099022 Tox120, RTL, 6M , G 1 ,4/19/99 22 042099023 Tox120, RTL, 7M, G 1 ,4/19/99 23 042099024 Tox120, RTL, 8M, G 1 ,4/19/99 24 042099025 Tox120, RTL, 9M, G 1 .4/19/99 25 042099026 Tox120, RTL, 10M , G 1 ,4/19/99 26 042099027 Tox120, R B L04199-250ppb-C C 2,4/19/99 27 042099028 MeOH Blank . 28 042099029 Tox120, RTL, 11M, G 1 ,4/19/99 29 042099030 Tox120, RTL, 13M, G 1 ,4/19/99 30 042099031 Tox120, RTL, 14M, G 1 .4/19/99 . 31 042099032 Tox120, RTL, 15M , G 1 ,4/19/99 32 042099033 Tox120, R B L04199-250ppb-C C 3,4/19/99 33 042099034 MeOH Blank 34 042099035 Tox120, RTL, 17M , G 2 .4/19/99 35 042099036 TOX120, RTL, 18M, G 2 ,4/19/99 36 042099037 Tox120, RTL, 19M , G 2 ,4/19/99 37 042099038 Tox120, RTL, 20M , G 2 .4/19/99 38 042099039 Tox120, RTL, 21M , G 2 ,4/19/99 39 042099040 Tox120. RBL04199-250ppb-CC4, 4/19/99 40 042099041 MeOH Blank 41 042099042 Tox120, RTL, 22M , G 2 ,4/19/99 42 042099043 T 0X I2O, RTL, 23M . G 2 ,4/19/99 43 042099044 T 0X I2O, RTL, 24M , G 2 ,4/19/99 44 042099045 T0X I2O, RTL, 25M , G 2 ,4/19/99 45 042099046 T 0X I2O, RTL, 26M , G 2 .4/19/99 46 042099047 TOX120, RBL04199-250ppb-C C 5,4/19/99 47 042099048 MeOH Blank 48 042099049 T 0X I2O, RTL, 27M , G 2 .4/19/99 49 042099050 T 0X I2O, RTL, 29M , G 2 ,4/19/99 50 042099051 T 0X I2O, RTL, 30M , G 2 ,4/19/99 51 042099052 Tox120, RTL, 31 M , G 2 ,4 /19/99 52 042099053 T 0X I2O, RBL04199-250ppb-C C 6,4/19/99 53 042099054 MeOH Blank 54 042099055 T 0X I2O, RTL, 33M , G 3 ,4/19/99 55 042099056 T0X I2O, RTL, 34M , G 3 ,4/19/99 56 042099057 T 0X I2O, RTL, 35M , G 3 .4/19/99 57 042099058 TOXI20, RTL, 36M , G 3 ,4/19/99 58 042099059 T 0X I2O, RTL, 37M , G 3 .4/19/99 59 042099060 T 0X I2O, RBL04199-250ppb-C C 7,4/19/99 60 042099061 MeOH Blank MS File In let File B o ttle 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min ' 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min . 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln . 9MIN-COMBI7IS-POAA 9mln 9MIN-COMB17IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMB17IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA - 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9M IN-CO M BI7IS-PO AA. 9mln 9MIN-COMBI7IS-POAA . 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 91 1 2 3 4 5 6 7 8 9 10 11. 91 12 13 14 15 16 81 92 17 18 19 20 21 82 92 22 . 23 24 25 83 92 26 27 28 29 . 30 84 92 31 32 33 34 35 85 92 36 37 38 39 86 93 40 41 42 43 44 87 93 Masslynx Sample List Sample List: C:\MASSLYNX\rats2.PRO\Sam pleDB\042099c.SPL Printed: Tue Apr 20 16:51:50 1999 Page 2 File Nam e 61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 6 6 042099067 67 042099068 6 8 042099069 69 042099070 70 042099071 71 042099072 72 042099073 73 042099074 74 042099075 75 042099076 76 042099077 77 042099078 78 042099079 79 042099080 80 042099081 81 042Q99082 82 042099083 83 042099084 84 042099085 85 042099086 8 6 042099087 87 042099088 8 8 042099089 89 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 96 042099097 97 042099098 98 042099099 99 042099100 1 0 0 042099101 1 0 1 042099102 1 0 2 042099103 103 042099104 104 042099105 F ile Text Tox120, RTL, 38M , G 3 ,4/19/99 Tox120, RTL, 39M , G 3 .4/19/99 Tox120, RTL, 40M , G 3 ,4/19/99 Tox120, RTL, 4 1 M . G 3 .4/19/99 Tox120, RTL, 42M , G 3 ,4/19/99 Tox120, RBL04199-250ppb-CC8,4/19/99 M eOH Blank Tox120, RTL, 43M , G 3 ,4/19/99 Tox120, RTL, 45M , G 3 ,4/19/99 Tox120, RTL, 46M , G 3 ,4/19/99 Tox120, RTL, 47M , G 3 ,4/19/99 Tox120, RBL04199-250ppb-CC9,4/19/99 M eOH Blank Tox120, RTL, 65M , G 5 ,4/19/99 Tox120, RTL, 66M , G 5 ,4/19/99 Tox120, RTL, 73M , G 5 ,4/19/99 Tox120, RTL, 74M , G 5 ,4/19/99 Tox120, RTL, 75M , G 5 ,4/19/99 Tox120, RBL04199-250ppb-CC4,4/19/99 . MeOH Blank Tox120, RTL, 77M , G 5 .4/19/99 Tox120, RTL, 78M , G 5 ,4/19/99 Tox120, RTL, 79M , G 5 ,4/19/99 Tox120, RBL04199-250ppt>-CC5,4/19/99 MeOH Blank Tox120, RTL, 3M , G1-250ppt>-MS, 4/19/99 Tox120, RTL, 3M . G1-250ppb-M SD, 4/19/99 Tox120, RTL, 6M , G1-250ppb-MS, 4/19/99 Tox120, RTL, 6M , G1-250ppt>-MSD, 4/19/99 Tox120, RTL, 13M , G1-250ppb-MS, 4/19/99 Tox120, RTL, 13M , G1-250ppb-M SD, 4/19/99 Tox120, RBL04199-250ppb-CC6,4/19/99 M eOH Blank Tox120, R B L04199-H20Blk-1,4 /1 9 /9 9 Tox120, RBL04199-UvBlk-1,4 /1 9 /9 9 Tox120, RBL04199-5ppb-1,4 /1 9 /9 9 Tox120, RBL04199-10ppb-1,4/19/99 Tox120, RBL04199-25ppb-1,4 /1 9 /9 9 TOX120, RBL04199-50ppt>-1,4/19/99 TOX120, RBL04199-100ppb-1,4 /1 9 /9 9 Tox120, RBL04199-250ppb-1,4 /1 9 /9 9 Tox120, RBL04199-500ppb-1,4/19/99 Tox120, RBL04199-750ppt>-1,4 /1 9 /9 9 Tox120, RBL04199*1000ppb-1,4 /1 9 /9 9 MS File In let File 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9M IN-COMBI7IS-POAA Omln 9MIN-COMBI7IS-POAA 9min ' 9M IN-COMBI7IS-POAA 9min 9M IN-COMBI7IS-POAA 9mln 9M IN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9M INtCOMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9m!n 9MIN-COMBI7IS-POAA 9mjn 9M IN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min . 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA : 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9M IN-COM BI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9M1N-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln B ottle 45 46 47 48 49 88 93 50 51 52 53 89 93 68 69 70 71 72 84 94 73 74 75 85 95 76 77 78 79 80 90 86 94 1. 2 3 4 5 6 7 8 9 10 11 Masslynx -Sample List / Sample List:C:\MASSLYNX\rats2.PRO\SampleDB\042099d.SPL Printed:Tue Apr2016:51:40 1999 .?i i FileName FileText 1 042099106 MeOH Blank I 2 042099107 Tox120, RBL04199-H2OBIk-1,4/19/99 i 3 042099108 4 042099109 Tox120, RBL04199-UvBlk-1,4/19/99 Tox120,RBL04199-5ppb-1,4/19/99 5 042099110 Tox120, RBL04199-10ppb-1,4/19/99 6 042099111 Tox120, RBL04199-25ppb-1,4/19/99 l 7 042099112 8 042099113 Tox120, RBL04199-50ppb-1,4/19/99 TOX120, RBL04199-100ppb-1,4/19/99 9 042099114 Tox120, RBL04199-250ppb-1,4/19/99 10 042099115 Tox120, RBL04199-500ppb-1,4/19/99 1 11 042099116 12 042099117 Tox120, RBL04199-750ppb-1,4/19/99 Tox120, RBL04199-1000ppb-1,4/19/99 13 042099118 MeOH Blank 14 042099119 TOX120, RTL, 49M, G4,4/19/99 1 15 042099120 16 042099121 TOX120, RTL, 50M, G4,4/19/99 Tox120, RTL, 51M, G4,4/19/99 17 042099122 TOX120, RTL, 52M, G4.4/19/99 T 18 042099123 19 042099124 20 042099125 Tox120, RTL, 53M, G4,4/19/99 TOX120, RBL04199-250ppb-CC1,4/19/99 MeOH Blank 21 042099126 Tox120, RTL, 54M, G4.4/19/99. 22 042099127 Tox120, RTL, 55M, G4.4/19/99 23 042099128 Tox120, RTL, 56M, G4,4/19/99 24 042099129 Tox120, RTL, 57M, G4,4/19/99 25 042099130 TOX120, RTL, 58M, G4,4/19/99 26 042099131 Tox120, RBL04199-250ppb-CC2,4/19/99 27 042099132 MeOH Blank . 28 042099133 Tox120, RTL, 59M, G4,4/19/99 29 042099134 Tox120, RTL, 61M, G4,4/19/99 30 042099135 Tox120, RTL, 62M, G4,4/19/99 31 042099136 Tox120, RTL, 63M, G4,4/19/99 32 042099137 Tox120, RBL04199-250ppb-CC3,4/19/99 33 042099138 MeOH Blank 34 042099139 .Tox120, RBL04199-H2OBIk-1,4/19/99 35 042099140 Tox120, RBL04199-UvBlk-1,4/19/99 J 36 042099141 37 042099142 38 042099143 Tox120, RBL04199-5ppb-1,4/19/99 Tox120, RBL04199-10ppb-1,4/19/99 Tox120, RBL04199-25ppb-1,4/19/99 39 042099144 Tox120, RBL04199-50ppb-1,4/19/99 40 042099145 Tox120, RBL04199-100ppb-1,4/19/99 ' J 41 042099146 42 042099147 TOX120, RBL04199-250ppb-1,4/19/99 TOX120, RBL04199-500ppb-1,4/19/99 43 042099148 Tox120, RBL04199-750ppb-1,4/19/99 44 042099149 TOX120, RBL04199-1000ppb-1,4/19/99 - . MSFIle y / ^ V B A C K TO MAIN > Page 1 ' . InletFile Bottle longB 91 long6 1 long6 2 long6 3 longB 4 longB 5 longB 6 longB 7 longB 8 long6 9 !ong6 10 long6 11 longB 93 longB 54 long6 55 longB 56 long6 57 long6 . 58 long6 81 long6 94 longB 59 long6 60 long6 61 long6 62 long6 63 long6 82 long6 94 long6 64 long6 65 long6 66 longB 67 longB 83 long6 94 long6 .1 * longB 2 ' long6 3 long6 ' 4 long6 5 long6 6 longB 7 longB 8 long6 9 longB 10 long6 11 Masslynx - Sample List i Sample List:C:\MASSLYNX\rats2.PRO\SampleDB\042099d.SPL Printed:Tue Apr 2016:51:401999 . !| I File Nam e FileText 1 042099106 MeOH Blank l 2 042099107 Tox120, RBL04199-H20Blk-1,4/19/99 i 3 042099108 Tox120, RBL04199-UvBlk-1,4/19/99 4 042099109 Tox120, RBL04199-5ppb-1,4/19/99 5 042099110 Tox120, RBL04199-10ppb-1,4/19/99 6 042099111 TOX120, RBL04199-25ppb-1,4/19/99 l 7 ' 042099112 8 042099113 Tox120, RBL04199-50ppb-1,4/19/99 Tox120, RBL04199-100ppb-1,4/19/99 9 042099114 Tox120, RBL04199-250ppb-1,4/19/99 10 042099115 Tox120, RBL04199-500ppb-1,4/19/99 1 11 042099116 12 042099117 Tox120, RBL04199-750ppb-1,4/19/99 Tox120, RBL04199-1000ppb-1,4/19/99 13 042099118 MeOH Blank 14 042099119 TOX120, RTL, 49M, G4,4/19/99 l 15 042099120 16 042099121 TOX120, RTL, 50M, 64,4/19/99 Tox120, RTL, 51M, G4.4/19/99 17 042099122 Tox120, RTL, 52M, G4.4/19/99 18 042099123 Tox120, RTL, 53M, G4.4/19/99 19 042099124 TQX120, RBL04199-250ppb-CC1,4/19/99 I 20 042099125 21 042099126 MeOH Blank Tox120, RTL, 54M, G4,4/19/99. 22 042099127 Tox120, RTL, 55M, G4,4/19/99 23 042099128 Tox120, RTL, 56M, G4,4/19/99 24 042099129 Toxi20, RTL, 57M, G4,4/19/99 25 042099130 Tox120, RTL, 58M, G4,4/19/99 26 042099131 TOX120, RBL04199-250ppb-CC2,4/19/99 27 042099132 MeOH Blank . 28 042099133 Tox120, RTL, 59M.G4,4/19/99 29 042099134 Tox120, RTL, 61M, G4,4/19/99 30 042099135 TOX120, RTL, 62M, G4,4/19/99 31 042099136 TOX120, RTL, 63M, G4,4/19/99 32 042099137 TOX120, RBL04199-250ppb-CC3,4/19/99 33 042099138 MeOH Blank 34 042099139 .TOX120, RBL04199-H20Blk-1,4/19/99 35 042099140 Tox120, RBL04199-UvBlk-1,4/19/99 J 36 042099141 37 042099142 38 042099143 TOX120, RBL04199-5ppb-1,4/19/99 Tox120, RBL04199-1Oppb-1,4/19/99 Tox120, RBL04199-25ppb-1,4/19/99 39 042099144 Tox120, RBL04199-50ppb-1,4/19/99 40 042099145 TOX120, RBL04199-100ppb-1,4/19/99 ~ 1 41 042099146 42 042099147 Tox120, RBL04199-250ppb-1,4/19/99 TOX120, RBL04199-500ppb-1,4/19/99 43 042099148 Tox120, RBL04199-750ppb-1,4/19/99 44 042099149 TOX120, RBL04199-1000ppb-1,4/19/99 ] I l !I y / ^ BACK TO MAIN - , Pagel ' '. / MS File PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 I PFBS-4 I PFBS-4 PFBS-4 ' PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 ' . PFBS-4 PFBS-4 , .PFBS-4 / PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 . PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 , PFBS-4 \ PFBS-4 \ PFBS-4 \ PFBS-4 1 PFBS-4 I PFBS-4 \ PFBS-4 \ PFBS-4 \ PFBS-4 \ ' InletFile Bottle long6 91 long6 1 longe 2 long6 3 long6 4 longe 5 long6 6 long 7 longe 8 longe 9 long6 10 long6 11 longe 93 longe 54 longe 55 longe 56 longe 57 long6 . 58 longe 81 longe 94 long6 59 long6 60 long6 61 long6 62 long6 63 longe 82 long6 94 long6 64 long6 65 longe 66 longe 67 long6 83 long6 94 longe .1 *longe 2 s long6 3 long6 ' 4 long6 5 long6 6 long6 7 longe 8 long6 9 longe 10 long6 11 ] 1 J ] J Scanning Method Saport M ethod! e:\MMSUMX\SAIS2.PltO\hCgDDS\9MZH-CCMBXTZS-MMh laat-Modified: Tue Apr 20 1:33:12 1999 ' Printed! Tue Apr 20 16i33:19 1999 Solvent Delay ( Mina ) ! 0.00 Function ! 1 MRM of 1 Maas Pair ( ISP- ) Inter Channel Delay ( Sue* ) t Span ( Deltona ) i Start Tie* ( Hina ) i Cnd Tina ( Mina ) > Repeata 1 . Channel Parent D.ught.r 0.03 0.00 0.00 9.00 1 Dwell (Sk i ) Coll Inorgy (V) COM `1 413.00 169.00 0.30 30 1$ Function : 2 MRM of 1 M u i Pair ( ISP- | Inter Chennai Daley ( Sacs ) t Span ( Deltona ) * Start Tina { Mins ) * End Tina ( Mins ) t Repeats t Chennai Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Sac*) . Coll Snargy (V) Con. 1 469.00 119.00 0.30 30 13 Function i 3 MSN of 1 Maaa Pair ( ISP- ) Inter Chennai Daley ( S.c. ) Span ( Deltona ) Start Tina { Mina ) : End Tina { Mina ) * Rapaats ; Chennai Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Seca) Coll In.rgy (V) Con* 1 569.00 119.00 0.30 30 IS Function : 4 MRM of 1 Maes Pair ( ESP- ) Intar Chennai Delay ( S.C* ) ! Span ( Daltons ) : Start Tina ( Mina ) s End Tima ( Kina ) : Repeats t Chennai Parent D.ught.r 0.03 0.00 0.00 9.00 1 Dwell (S.c*) Coll tnargy (V) Con. 1 419.00 119.00 0.30 30 15 Function r 5 MRM of 1 Mess Pair ( ZIP- ) Inter Chennai Delay ( Secs ) t Span ( Daltons ) t Start Tima { Mins ) : End Tima { Hina ) j Rapaats i Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (S.C.) Coll Energy (V) Co m 1 S19.00 119.00 0.30 30 Funestion z C ' MRM of 1 Mass Pair ( U P - ) IS - Inter Channel Delay ( Secs ) t Span ( Daltons ) : Start Time ( Mina ) i End Time ( Mina ) 1 Repeats t Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Sua) Coll In.rgy (V) Co m 1 499.00 99.00* 0.30 43 *0 Function t ? MRM of 1 Mass Pair ( ISP- ) Inter Channel Delay ( 3ca ) ! Span ( Daltons ) s Start Time < Mins ') s End Tine ( Mina ) i Repeats i Channel Parent Daughter 0.03 0.00 0.40 9.00 1 Dwell (Sial Coll Enargy <VJ Con. 1 399.00 0.00 0.30 43 60 Function i 1 MRM of 1 Maas Pair { u p - ) Inter channel Delay ( Sacs ) t Span ( Daltons ) t Start Tims ( Mina } : End Tims ( Mina ) t Repeats s Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (S.C*) Coll Enrgy (V) Con. 1 427.00 60.00 0.30 40 60 7 / * BACK TO MAIN Page 1 Scanning Method Report Mathod: C :\MASSLYXX\RATS2. PRO\ACQUCB\9KIN-CCMBI7IS-POAA Laat"Modifiad: Tua Apr 20 16:33:12 1999 Printad: Tua Apr 20 16:33:19 1999 Solvant Delay ( mina ) i 0.00 Function 1 1 M M of 1 M u a Pair ( ISP- ) Intar Channal Dalay ( Sacs ) t Span ( Daltons } : Start Tima ( Hina ) : End Tima ( Hina ) : Repasta i Channal Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Sacs) Coll Energy (V) Cona ( V ) *1 413.00 169.00 0.30 30 15 function : 2 MKM of 1 Haas Pair I ISP- ) Inter Channal Dalay ( Secs ) t Span ( Daltons ) t Start Time ( Mina ) : End Tima ( Mina ) : Repeats : Channal Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Sacs) . Coll Energy (V) Cona ( V ) 1 469.00 119.00 0.30 30 15 function : 3 M M of 1 Mass Pair ( ESP- | . - Inter Chann.1 Dtlay ( Secs ) s Spin ( Dalton* ) I Start Tin* ( Hina ) I End Tin* l Mina ) i Repeats ! Channal Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Sacs) Coll Energy leV) Cona ( V ) 1 569.00 119.00 0.30 30 15 function : 4 M M of 1 Maas Pair ( ESP- ) Intar Channal Dalay ( Sacs ) t Span ( Daltons ) : start Tima ( Hina ) : End Tima ( Mins ) t Repeats : Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (V) Cona ( V J 1 419.00 119.00 0.30 30 15 function : 5 MRM of 1 M u a Pair ( ESP- | Inter Channel Delay ( Sac* ) i Span ( Daltons ) : Start Time ( Kins ) : End Time ( Mins ) : Repeats : Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (*V) Cona ( V ) 1 519.00 119.00 0.30 30 j Function : C ' MRM of X M u e Fair ( ESP- ) 15 Inter Channel Delay ( Sacs ) s Span { Daltons ) : Start Tima ( Mins ) : End Tima ( Mins ) : Repeats : Channal Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Sees) Coll Energy (*V) Cona ( V ) 1 499.00 99.00 0.30 45 60 Function i 7 M M of 1 Maaa Pair ( ESP- J Inter Channal Dalay ( Sacs ) t Span ( Daltons ) : Start Tia* ( Hina ') t End Tlaa ( Kins ) > Rapaats t - Channal Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (*V) Cona ( V ) 1 399.00 80.00 0.30 45 60 Function i 8 M M of 1 Mnas Pair ( ISP- ) Inter Channal Delay ( Sacs ) { Span ( Dalton* ) : Start Tin* ( Mins ) : End Tina ( Min* ) I Repeats t Channal Parant Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (V) Cona ( V ) 1 427.00 80.00 0.30 40 60 .. / " BACK TO MAIN * Pag* 1 ' ' A '1 canning Mathod R eport Matho d : C :\M M SH IK \R A T a2.FR O \A C Q O O S\ L aaJ Modif i ad : Non Mar IS 14:22:03 1999 l>rintad: Tua Apr 20 17:03:29 1K 9 -4 - lolvint Dalay ( Kina ) 1 0.00 funetien : 1 MRM of 4 Maaa valra ( H P - ) Intar Channal Dairy ( Sacs ) : Span ( Daltons ) : Start Tina (Mina ) *1 End Tina ( Mina ) 2 Rapeata : Chahnal Parent Daughtar 0.03 0.00 0.00 13.SO 1 Duali (Sacs) Coll Energy (aV) Con. (V ) *1 2 3 299.00 *80.00 299.00 99.00 299.00 130.00 0.30 0.30 0.30 45 45 45 60 60 60 y BACK TO MAIN * Paga I AMELIA062498: FACT Equipment Log 99016 Q uattro II, #6448E BACK TO MAIN /yu?& PAGE DATE: 04/22/99 . ANALYST: mee ' STUDY NUM BER Tox-120 M ATRIX: R at Liver DESCRIPTION: ip FACT M ethod: ETS-8-S.0________ Injection volum e, lO uL Cycle tim e: 13.5 m in. M obile Phase: (A)2.0 m M NH4CH3CQ2 Electrode: Z-Spray (B)M cO H TN-A- or stndrd num ber (A) 99012-5-22_________ (B) TN-A-2414 Prepared (date/by): (A) 4/16/99 / ok_________ (B j 4/9/99 hoi Expires: (A) 10/99_______________ (B) 10/99________ Store at room tem p? [YJ or N O ther G u ard colum n: N one __________________ Part number: na Lot num ber na A nalytical colum n: keystone betasil____________________________________________ Size: 2 x 50mm_________________ Type: c !8____________ Sphere size: 5 um Part num ber 055-701-2 ' L ot n um ber P7L04 Serial num ber 0481172z A nalytical p aram eters: t Drying gas (L/hr): 300 _ ESI nebulizing gas (L/hr) 15 LC Flow rate: 300 uL/m in. Back pressure: 59 bar . H PLC solvent g rad ien t: long2 Tim e (m in.) 1) 0.00____________ 2) 8.5_____________ .3 11.0____________ 5) 12.0____________ 6) 13.5_________ %B 40 90 90 40 stop Sheath gas (X/hr) 0 BACK TO MAIN <//**/?? _ AMELIA062498: FACT Equipment Log 99016 Q uattro H , #6448E PAGE______ DATE: 04/22/99 ANALYST: mee ' . STUDY NUMBER Tox-120 MATRIX: R at Liver (pfbs) DESCRIPTION: ip FACT M ethod: ETS-8-S.0________ Injection volum e,10 uL Cycle time: 15 min. Electrode: Z-Spray M obile P hase: (A)2.0 mM NH4CH3CQ2 (B)M eO H TN-A- or stndrd num ber (A) 99012-5-22_________ (B) TN-A-2414 Prepared (date/by): (A) 4/16/99 / ok_________ ' (B) 4/9/99 hoi Expires: (A) 10/99_______________ (B) 10/99 Store at room tem p?' [Y] or N O th er:__ G u ard colum n: None '_________________ Part number: na Lot number: na A nalytical colum n: keystone betasil___________________________ .________________ Size: 2 x 50mm_________________ Type: c !8____________ Sphere size: 5 um Part number: 055-701-2 Lot number: P7L04 Serial number: 0481172z A nalytical param eters: Drying gas (L/hr): 300 ESI nebulizing gas (L/hr) 15 LC Flow rate: 300 uL/min. Back pressure: 59 bar Sheath gas (L/hr)_0 H PLC solvent g rad ien t: long2 Time (m in.) %B 1) 0.00_______________ 25 2) 5.0_____________ 95 3 12.0 95 5) 13.5 25 6) 15__________ stop BACK TO MAIN / > 0 t? Tuning Method Report . Method: C :\MASSLYNX\RATS2. PRO\ACQUDB\PFOS Printed: Thu Apr 22 16:25:24 1999 AiwJfc OLMI Page 1 lA t o ilh ill SOURCE ( ESP- ) Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp. Set Rdbk 3.00 15 5 .0.2 150 250 2.96 -14 -5 - 152 253 MSI LM Resolution HM Resolution - Ion Energy Ion Energy Ramp Lens 6 Multiplier Pressures Analyser Vacuum Gas Cell Rdbk . 2.0e-4 5.5e-3 . .s MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier Set ' 15.0 15.0 2 -0 $ 0.0 . 5 650 Set Rdbk 15.0 15.0 . 30 32 2.0 0.0 40 39 0 31 650 BACK TO MAIN Masslynx Sample List Sample List C:\MASSLYNX\rats2.PRO\SampleDB\042299a.SPL Printed:Tilt.Apr22 17:50:31 1999 , ' Idiilit, ftf, Page 1 File N a m e FileText . 1 042299001 MeOH Blank 2 042299002 Tox120, RBL04199-H20Blk-1,4/19/99 3 042299003 Tox120, RBL04199-LivBlk-1,4/19/99 4 042299004 Tox120, RBL04199-5ppb-1,4/19/99 5 042299005 Tox120, RBL04199-10ppb-1,4/19/99 6 042299006 Tox120, RBL04199-25ppb-1,4/19/99 7 042299007 Tox120, RBL04199-50ppb-1,4/19/99 8 042299008 Tox120, RBL04199-100ppb-1,4/19/99 i 9 042299009 Tox120, RBL04199-250ppb-1,4/19/99 10 042299010 Tox120, RBL04199-500ppb-1,4/19/99 11 042299011 Tox120, RBL04199-750ppb-1,4/19/99 12 042299012 Tox120, RBL04199-1000ppb-1,4/19/99 13 042299013 MeOH Blank 14 042299014 Tox120, RTL, 49M, G4,4/19/99, D10 15 042299015 Tox120, RTL, 50M, G4,4/19/99, D10 16 042299016 Tox120, RTL, 51M, G4,4/19/99, D10 17 042299017 Tox120, RTL, 52M, G4,4/19/99, D10 18 042299018 Tox120, RTL, 53M, G4,4/19/99, D10 19 0422990-19 Tox120, RBL04199-250ppb-CC1,4/19/99 . 20 042299020 MeOH Blank 21 042299021 Tox120, RTL, 54M, G4.4/19/99, D10 22 042299022 Tox120, RTL, 55M, G4,4/19/99, D10 23 042299023 Tox120, RTL, 56M, G4,4/19/99, D10 24 042299024 Tox120, RTL, 57M, G4,4/19/99, - 25 042299025 Tox120, RTL, 58M, G4,4/19/99, 26 042299026 Tox120, RBL04199-250ppb-CC2,4/19/99 27 042299027 MeOH Blank - 28 042299028 Tox120, RTL, 59M, G4,4/19/99, 29 042299029 Tox120, RTL, 61M.G4,4/19/99, 30 042299030 Tox120, RTL, 62M, G4,4/19/99, . 31 042299031 Tox120, RTL, 63M, G4,4/19/99, 32 042299032 Tox120, RBL04199-250ppb-CC1,4/19/99 33 042299033 MeOH Blank - 34 042299034 Tox120, RBL04199-H2OBIk-1,4/19/99 35 042299035 Tox120, RBL04199-UvBlk-1,4/19/99 36 042299036 Tox120, RBL04199-5ppb-1,4/19/99 37 042299037 Tox120, RBL04199-10ppb-1,4/19/99 38 042299038 . Tox120, RBL04199-25ppb-1,4/19/99 39 042299039 Tox120, RBL04199-50ppb-1,4/19/99 40 042299040 Tox120, RBL04199-100ppb-1,4/19/99 41 042299041 Tox120, RBL04199-250ppb-1,4/19/99 42 042299042 Tox120, RBL04199-500ppb-1,4/19/99 43 042299043 Tox120, RBL04199-750ppb-1,4/19/99 44 042299044 Tox120, RBL04199-1000ppb-1,4/19/99 M S File 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15M1N 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15M1N 15MIN 15MIN 15MIN 15M1N 15MIN InletFile Bol long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 a long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 91 1 2 3 4 5 6 7 8 9 10 11 91 12 13 14 15 16 17 91 18 19 20 21 22 23 91 24 25 26 27 17 92 1, 2 3 4 5 6 -7 8 9 10 11 y/z i BACK TO MAIN Masslynx Sample List Sample List:C:\MASSLYNX\rats2.PRO\SampleDB\042299b.SPL Printed:Thu Apr2217:46:29 1999 4^ . Page 1 M C b i / b / i l ___________________ ____ File N a m e FileText MS File InletFile 1 042299045 MeOH Blank 13MIN-COMBI7IS-POAA long2 2 042299046 Tox120, RBL04199-H2OBIk-1,4/19/99 13MIN-COMBI7IS-POAA long2 3 042299047 Tox120, RBL04199-UvBlk-1,4/19/99 13MIN-COMBI7IS-POAA long2 4 042299048 Tox120, RBL04199-5ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 5 042299049 . Tox120, RBL04199-10ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 6 042299050 Tox120, RBL04199-25ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 7 042299051 Tox120, RBL04199-50ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 8 042299052 Tox120, RBL04199-100ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 9 042299053 Tox120, RBL04199-250ppb-1,4/19/99 . 13MIN-COMBI7IS-POAA long2 10 042299054 Tox120, RBL04199-500ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 11 042299055 Tox120, RBL04199-750ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 12 042299056 Tox120, RBL04199-1000ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 13 042299057 MeOH Blank 13MIN-COMBI7IS-POAA long2 14 042299058 Tox120, RTL, 17M, G2,4/19/99 13MIN-COMBI7IS-POAA long2 15 042299059 Tox120, RTL, 18M, G2,4/19/99 13MIN-COMBI7IS-POAA long2 16 042299060 Tox120, RTL, 19M, G2,4/19/99 13MIN-COMBI7IS-POAA long2 17 042299061 Tox120, RTL, 20M, G2,4/19/99 13MIN-COMBI7IS-POAA long2 18 042299062 Tox120, RTL, 21M, G2,4/19/99 13M IN-COMBI7IS-POAA long2 19 042299063 Tox120, RBL04199-250ppb-CC4,4/19/99 . 13MIN-COMBI7IS-POAA long2 20 042299064 MeOH Blank 13MIN-COMBI7IS-POAA long2 21 042299065 Tox120, RTL, 22M, G2,4/19/99 13MIN-COMBI7IS-POAA long2 22 042299066 Tox120, RTL, 23M, G2,4/19/99 13MIN-COMBI7IS-POAA long2 23 042299067 Tox120, RTL, 24M.G2,4/19/99 . 13MIN-COMBI7IS-POAA long2 24 042299068 Tox120, RTL, 25M, G2,4/19/99 13MIN-COMBI7IS-POAA long2 25 042299069 26 042299070 Tox120, RTL, 26M.G2,4/19/99 Tox120, RBL04199-250ppb-CC5,4/19/99 13MIN-COMBI7IS-POAA long2 13MIN-COMBI7IS-POAA long2 27 042299071 MeOH Blank . 13MIN-COMBI7IS-POAA long2 28 042299072 Tox120, RTL, 27M, G2,4/19/99 13MIN-COMBI7IS-POAA long2 29 042299073 Tox120, RTL, 29M, G2, 4/19/99 13MIN-COMBI7IS-POAA long2 30 042299074 Tox120, RTL, 30M, G2.4/19/99 13M IN-COMBI7IS-POAA long2 31 042299075 Tox120, RTL. 31M, G2,4/19/99 13MIN-COMBI7IS-POAA long2 32 042299076 Tox120, RBL04199-250ppb-CC6,4/19/99 13M IN-COMBI7IS-POAA long2 33 042299077 MeOH Blank 13MIN-COMBI7IS-POAA long2 34 042299078 Tox120, RTL, 33M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 35 042299079 Tox120, RTL, 34M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 36 042299080 Tox120, RTL, 35M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 37 042299081 Tox120, RTL, 36M, G3,4/19/99 13MIN-COMBI7IS-POM long2 38 042299082 Tox120, RTL, 37M.G3,4/19/99 13MIN-COMBI7IS-POAA long2 39 042299083 Tox120, RBL04199-250ppb-CC7, 4/19/99 13M IN -COM BI7IS-POAA long2 40 042299084 MeOH Blank . 13MIN-COMBI7IS-POAA long2 41 042299085 Tox120, RTL, 38M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 42 042299086 Tox120, RTL, 39M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 43 042299087 Tox120, RTL, 40M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 44 042299088 Tox120, RTL, 41M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 45 042299089 Tox120, RTL, 42M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 46 042299090 Tox120, RBL04199-250ppb-CC8,4/19/99 13MIN-COMBI7IS-POAA long2 47 042299091 MeOH Blank 13MIN-COMBI7IS-POAA long2 48 042299092 Tox120, RTL, 43M, G3.4/19/99 13MIN-COMBI7IS-POAA long2 49 042299093 Tox120, RTL, 45M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 50 042299094 T0XI2O, RTL, 46M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 51 042299095 Tox120, RTL, 47M, G3,4/19/99 13MIN-COMBI7IS-POAA long2 52 042299096 Tox120, RBL04199-250ppb-CC9,4/19/99 13MIN-COMBI7IS-POAA long2 53 042299097 MeOH Blank 13MIN-COMBI7IS-POAA long2 54 042299098 Tox120, RBL04199-H20Blk-1,4/19/99 13MIN-COMBI7IS-POAA long2 55 042299099 Tox120, RBL04199-LivBIk-1,4/19/99 13MIN-COMBI7IS-POAA long2 56 042299100 Tox120, RBL04199-5ppb-1,4/19/99 * 13MIN-COMBI7IS-POAA long2 57 042299101 Tox120, RBL04199-10ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 58 042299102 Tox120, RBL04199-25ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 59 042299103 Tox120, RBL04199-50ppb-1,4/19/99 13MIN-COMBI7IS-POM long2 60 042299104 Tox120, RBL04199-100ppb-1,4/19/99 13MIN-COMBI7IS-POAA long2 Bottle 91 1 2 3 4 5 6 7 8 9 10 11 91 31 32 33 34 35 84 92 36 37 38 39 40 85 92 41 42 43 44 86 93 45 46 47 48 49 87 93 50 51 52 53 54 88 93 55 56 57 58 89 93 1 2 3 4 5 6 7 Masslynx Sample Ust Sample List:C:\MASSLYNX\rats2PRO\SampleDB\042299b.SPL Printed:Thu Apr2217:46:29 1999 A Amliz Q L 2M BACK TO MAIN z? Page 2 Me or/itjw File Name 61 042299105 62 042299106 63 042299107 64 042299108 66 67 68 69 70 71 72 73 74 FileText Tox120, RBL04199-250ppb-1,4/19/99 Tox120, RBL04199-500ppb-1,4/19/99 Tox120, RBL04199-750ppb-1,4/19/99 Tox120, RBL04199-1000ppb-1,4/19/99 M S File InletFile 13MIN-COMBI7IS-POAA long2 13MIN-COMBI7IS-POAA long2 13MIN-COMBI7IS-POAA long2 13MIN-COMBI7IS-POAA long2 Bottle 8 9 10 11 BACK TO MAIN Scanning Method Report Method: C:\MASSLTHX\RAT82.PRO\ACQUDB\13MZM-CCMBZ7ZS-POAA Last Modified! Thu Apr 22 18:50:10 1999 . Printed: Thu Apr 22 18:50:18 1999 eJn Solvant Daisy ( aina ) : 0.00 function : 1 MRH of 1 Mass Pair ( ISP- ) Intar Channel Delay ( Sacs ) i Span ( Daltons ) Start Tint I Mins ) t End Time ( Mins ) : Repeats t Channel Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell (Secs) Coll Energy (aV) Cone ( V ) 413.00 169.00 0.30 function : 2 MRM of 1 Maes Pair ( ESP- ) Inter Channel Delay ( Secs ) : Span ( Daltons ) : Start Time ( Mins ) : End Time ( Mins ) : Repeats : Channel Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell . (Secs) Coll Energy (eV) * Cone ( V ) 1 469.00 119.00 0.30 45 ' 35 function : 3 MRM of 1 Maas Pair ( ESP- ) Inter Channel Delay ( Secs ) t Span ( Daltons } : Start Time ( Mins ) : End Time ( Mins ) * Repeats i Channel Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell (Secs) Coll Energy (eV) Cone ( V ) 1 569.00 119.00 0.30 45 35 function s 6 M m of 1 Mass Pair t EBP- ) Inter Channel Delay ( Sacs ) i Span ( Daltons ) : Start Time ( Mins } ; End Time ( Mins ) : Repeats t Channel Parent Daughtsr 0.03 0.00 0.00 13.50 Dwell (Secs) Coll Energy (eV) Cone ( V 1 419.00 119.00 0.30 45 35 function : 5 MRM of 1 Mass Pair ( E8P- ) Intsr Channel Delay ( Secs ) : Span { Daltons ) : Start Tiae ( Mins ) : End Time ( Mins ) : Repeats : * Channel Parent Daughter 0.03 0 .0 0 0.00 13.SO 1 Dwell (5ecs) * Coll Energy (eV) i 519.0 119.00 0.30 45 Cone ( V ) 35 function : 6 * MRM of 1 Mass Pair ( ESP- ) Inter Channel Delay ( Seca ) : Span ( Daltons ) : Start Time ( Mins ) t End Time ( Mins ) : Repeats i Channel Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell (Secs) Coll Energy tv> Cone 1 499.00 99.00* 0.30 45 80 Function > 7 M M of 1 M... Pair ( ESP- ) Inter Channel Delay ( Secs ) t Span ( Daltons ) st Start Time ( Mins ) t End Time ( Mins ) t Repeats : Channel Parent Daughter 0.03 0.00 0.00 13.50 1 Dwell (Secs) Coll Energy (V) Cone 1 399.00 60.00 0.30 45 80 Function i M M of 1 H U I Pair ( ESP- ) Inter Channel Delay ( Secs ) : Span ( Daltorn ) : Start Time ( Mina ) j End Time ( Mins ) : Repeats : Channel Parent Daughter 0.03 0.00 0.00 13.50 1 (Veil (Sec*) Coll Energy (VI Cone 1 427.00 80.00 0.30 40 60 Q i t W L ______ or.ifJit 3? Pag* 1 Scanning Mathod Report _- Method: C :\HAS8LYNX\RAT82. FSO\ACQUDB\15MXM Last Modified: Thu Apr 22 15:32:30 1999 Printed: Thu Apr 22 15:52:34 1999 -4 nvlA tLViV Solvent Delay ( nine ) : 0.00 Function t 1 MRM of 4 Mass Pairs C ESP- ) * Intar Channal Oalay ( Secs ) : Span ( Daltons ) i Start Time { Mins ) : End Time ( Mins ) : Repeats : Channel Parent Daughter 0.03 0.00 0.00 15.00 1 Dwell (Secs) Coll Energy (eV) Cone ( V ) 1 2 3 .4 299.00 299.00 299.00 299.00 80.00 99.00 130.00 180.00 0.30 0.30 0.30 0.30 45 45 45 45 60 60 60 60 TM BACK TO MAIN rf/^/yy L sh M __________ Pag. 1 ] Scanning Method Report Method! Cl\MASSI.ln0C\RATS2.PRO\AC0UDB\l l u t M odified: Thu Apr 22 19>52:S0 199 P rin te d : Thu Apr 22 15i52:54 1999 Amelia nLM/tt ! Solvent Daisy ( Bins ) I 0.00 Punatlea i 1 M M of 4 Maaa P a i n ( U P - ) ' Intar Channal Dalay ( Sacs ) i Span ( Daltona ) Start T i n ( Mina ) i End Tlaa ( Mina ) t Aapaata : Channal Parant Daughter 0.03 ' 0.00 o.oo 15.00 1 Dwell (Steal coll Energy (aV) Cone ( V ) 1 299.00 80.00 0.30 2 299.00 99.00 0.30 3 299.00 130.00 0.30 4 299.00 180.00 0.30 45 45 45 45 80 0 0 60 " " BACK TO MAIN rf/^/yy Pug* 1 LAC, O x / m / t f ' ' BACK TO MAIN I FACT: DATA REVIEW SUMMARY FORM 1 4/3/$9 . 1 I ] 3 1 T J J 1 ] ] D oJfiU j ( a . c A u / m / a - j loo f x j U - A ^ y i i / d h i ^ r e c t ^ i 0V-. f / 2 .5 / 3 3 J I ] Date: 1 I 1 1 1 ! ] J ] ! J I J i I FACT: DATA REVIEW SUMMARY FORM 4/2-3/99 BACK TO MAIN ' *2* O x m a m , , t o hx. pl&Jrt n h y r tc fa A . V W s fcjv- 4 / 2 - 5 / 3 3 / \ Amila 062498: Raviavad and quantitatad by kjh 4/23/99 f i 03 > O * O > Quantify Calibration Raport Calibration: C:\MMSLTOC\rata2.PRO\CurvaD8\04229ta Last nodifiad: Frl Apr 23 08:51:34 1999 Prlntad: frl Apr 23 08:52:40 1999 ' " BACK TO MAIN , Vaga 1 . ' Amalia 062498: Ravlawad and quantittad by k)h 4/23/99 BACK TO MAIN ] ! M atta# R epert M attala ( H a i Laet n a tifla ti eaaVe w i ^ - frltta # i f i V ernila k M t f a frH \ra til. < a \M a tta 0 \0 4 ta .a # f r i A#c 23 M i H i 41 l i t i o Il N m Hay 10 1 i M i 20 Jltfft CtfOMW # R a m i le ta m a i R a f.i Cm m . ( RM4rt*i `IH a ao t C*m . A UUaemr'MNikV afimMti MI t a t t i n t * h m m f a e te ri 1.000000a 1 .0 0 0 0 0 0 , M a t t i a # 1.00 t" >*> . IttN I tlM TSM IS lM ll M U U n R e ta tila T IM I t e MlrtMw l a i M l i - teak M m U m i U N , iM k M 0 .0 0 0 . M aahla# #.*# . RMrut ' bearmi ' A rea *' N ly m a ltl Typai h lM a l O rio lai Ut Naloatinfl ' M ia T rate fam a tie n i 9ttmaav\m U nitat R atea feak * te-# eak W R llM a i L im ar 1M a l a t e /* Barn 4M Rumar al tataUii mattino ette# Ja U v a lla r tkreehel#i M u w Raak t a l i Ino < R atea 0 M e lin a a i i l w a i tuUaaoUfi O rw v a rtla a l ttrm h e l# ! B ataat ahaalO arai D a u ia a r th reah el# l R e la tiv a R alfO ti M ie la te M io tti R e lativ a a rm i M ia lu ta a rm i . 2 to.oo M .O O 0.00 1.0 0 , M eati# # 0 .0 0 , M e a ti a# 2 .0 0 , M e a ti a# 0 .0 0 , M aatla# t A ivJ/A Ms ZH W /a c . or//ft/ l _______ M H* l SOUP 020199: FACT Equipment Log 99014 Q uattro II, #6414E PAGE DATE: 05/14/99 .. ANALYST: drb ' STUDY NUMBER: Tox-120 MATRIX: R at L iver DESCRIPTION: dilutions FACT M ethod: ETS-8-5.0 . Injection volume: 10 uL Cycle time: 9 min. Electrode: Z-Sprav______ M obile Phase: (A12.Q mM NH4CH3CQ2 TN-A- or stndrd number (A) 99011-6-9__________ Prepared (date/by): (A)04/28/99 drb________ Expires: (A) 10/99 ________ _ OBI MeOH (B) TN-A-2506 (Bl 04/26/99 drh fBl 10/99 Store at room temp? 0 or N Other:________ ;__________._____ G u ard colum n: None__________________ P art number: na_________ Lot number: na________________________ A nalytical colum n: Keystone B etasil____________________________________ Size: 2 x 50 mm ___________ Type: C18____________ Sphere size: 5 um______ Part number: 055-701-2__________ Lot num ber P7LQ4 Serial number:0481175Z A nalytical param eters: Drying gas (L/hf): 350 ESI nebulizing gas (L /hr) 15 LC Flow rate: 3 0 0 11T/m in. Back pressure: 55 bar Sheath gas (L/hr)_0 H PLC solvent gradient: 9m in-2 Time /m in.l D 0.00__________ 2 1 1.00______________ 3 ) A5Q___________ 4) JL5Q__________ %B___________ 4 0 Q ____________ j JOO________ J 1 0 _______ 2 1 0 _______ . BACK TO MAIN BACK TO MAIN Page 1 MS SOURCE ( ESP- ) Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Ten. Set Rdbk 3.00 60 5 0.2 150 250 2.98 -29 -7 153 248 Pressures . Analyser Vacuum Gas Cell Rdbk 3.3e-5 4 .3e-3 MSI LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier Set Rdbk 14.0 14.0 , 2.0 * . 0.0 56 650 MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier ' Set Rdbk 15.0 15.0 45 2.0 0.0 40 0 650 42 40 44 BACK TO MAIN Masslynx - Sample List Sample List:C:\MASSLYNX\FACT-1.PROVSampleDB\05t499c.SPL Printed: FriMay 1416:17:21 1999 wm Page 1 FileNami FileText . 1 05149906 MeOH Blank 2 05149906 MeOH Blank 3 05149906 Tox-120, RBL04199-H20Blk-2,4/19/99 4 05149906 Tox-120, RBL04199-LBIk-2,4/19/99 5 05149906 Tox-120, RBL04199-5ppb-2,4/19/99 6 05149906 Tox-120, RBL04199-1Oppb-2,4/19/99 7 05149906 Tox-120, RBL04199-25ppb-2, 4/19/99 8 05149906 Tox-120, RBL04199-50ppb-2, 4/19/99 9 05149906 Tox-120, RBL04199-100ppb-2,4/19/99 10 05149907 Tox-120, RBL04199-250ppb-2,4/19/99 11 05149907 Tox-120, RBL04199-500ppb-2, 4/19/99 12 05149907 Tox-120, RBL04199-750ppb-2,4/19/99 13 05149907 Tox-120, RBL04199-1000ppb-2,4/19/99 14 05149907 MeOH Blank 15 05149907 Tox-120 23M G2/D1:25 16 05149907 Tox-120 24M G2/D1:25 17 05149907 Tox-120 25MG2/D1-.25 18 05149907 Tox-120 26M G2/D1:25 19 05149907 Tox-120, RBL04199-250ppb-CC1,4/19/99 20 05149906 MeOH Blank 21 05149908 Tox-120 33M G3/D1:25 22 05149908 Tox-120 34M G3/01:25 23 05149908 Tox-120 35M G3/D1:25 24 05149908 Tox-120 36M G3/D1:25 25 05149908 Tox-120 37MG3/D1:25 26 05149908 Tox-120 38M G3 /0125 27 05149908 Tox-120 39M G3 /D1:25 28 05149908 Tox-120 40M G3/D125 29 05149908 Tox-120, RBL04199-250ppb-CC3,4/19/99 30 05149909 MeOH Blank 31 05149909 Tox-120 27MG2/D1:10 32 05149909 Tox-120 29MG2/01:10 33 05149909 Tox-120 30MG2/D1:10 34 05149909 Tox-120 31M G2/D1:10 35 05149909 Tox-120, RBL04199-250ppb-CC4,4/19/99 36 05149909 MeOH Blank 37 05149909 Tox-120 42M G3/D1:10 38 05149909 Tox-120 43M G3/D1:10 39 05149909 Tox-120 45M G3/D1:10 40 05149910 Tox-120 46M G3/D1:10 ' 41 05149910 Tox-120 47MG3/D1:10 42 05149910 Tox-120, RBL04199-250ppb-CC5,4/19/99 43 05149910 MeOH Blank 44 05149910 Tox-120, RBL04199-H20Blk-2,4/19/99 45 05149910 Tox-120, RBL04199-LBIk-2,4/19/99 46 05149910 Tox-120, RBL04199-5ppb-2,4/19/99 47 05149910 Tox-120, RBL04199-1Oppb-2,4/19/99 48 05149910 Tox-120, RBL04199-25ppb-2, 4/19/99 49 05149910 Tox-120, RBL04199-50ppb-2, 4/19/99 50 05149911 Tox-120, RBL04199-100ppb-2,4/19/99 51 05149911 Tox-120, RBL04199-250ppb-2,4/19/99 52 05149911 Tox-120, RBL04199-500ppb-2,4/19/99 53 05149911 Tox-120, RBL04199-750ppb-2,4/19/99 54 05149911 Tox-120, RBL04199-1000ppb-2,4/19/99 M S File inletI Botti ilume Sample T Cone A TELMER 9min- 96 10 Analyte TELMER 9min- 96 10 Analyte TELMER 9min- 41 10 Analyte TELMER 9min- 42 10 Analyte TELMER 9min- 43 10 Standard 4.93 TELMER 9min- 44 10 Standard 9.76 ' TELMER 9min- 45 10 Standard 24.8 TELMER 9min- 46 10 Standard 49.3 TELMER 9min- 47 10 Standard 97.6 TELMER 9min- 48 10 Standard 248 TELMER 9min- 49 10 Standard 493 TELMER 9min- 50 10 Standard 735 TELMER 9min- 51 10 Standard 976 TELMER 9min- 96 10 Analyte TELMER 9min- 52 10 Analyte TELMER 9min- 53 10 Analyte TELMER 9min- 54 10 Analyte TELMER 9min- 55 10 Analyte TELMER 9min- 56 10 Analyte TELMER 9min- 96 10 Analyte TELMER 9min- 57 10 Analyte TELMER 9min- 58 10 Analyte TELMER 9min- 59 10 Analyte TELMER 9min- 60 10 Analyte TELMER 9min- 61 10 Analyte TELMER 9min- 62 10 Analyte TELMER 9min- 63 10 Analyte TELMER 9min- 64 10 Analyte TELMER 9min- 65 10 Analyte TELMER 9min- 97 10 Analyte TELMER 9mn- 66 10 Analyte TELMER 9min- 67 10 Analyte TELMER 9min- 68 10 Analyte TELMER 9min- 69 10 Analyte TELMER 9min- 70 10 Analyte TELMER 9min- 97 TE L M E R 9min- 71 10 Analyte 10 Analyte TELMER 9min- 72 10 Analyte TELMER 9min- 73 10 Analyte TELMER 9min- 74 10 Analyte TELMER 9min- 7 5 " 10 Analyte TELMER 9min- 76 10 Analyte TELMER 9min- 98 10 Analyte TELMER 9min- 41 10 Analyte TELMER 9min- 42 10 Analyte TELMER 9min- 43 10 Standard 4.93 TELMER 9min- 44 10 Standard 9.76 TELMER 9min- 45 10 Standard 24.8 TELMER 9min- 46 10 Standard 49.3 TELMER 9min- 47 10 Standard 97.6 TELMER 9min- 48 10 Standard 248 TELMER 9min- 49 10 Standard 493 TELMER 9min- 50 10 Standard 735 TELMER 9min- 51 10 Standard 976 Factor 1 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.0O 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 Function i 1 MRU of 3 H u s sirs ( S O - ) Intar Channel Delay ( Secs ) i Span ( Daltons ) t Start Tine { Kins ) * End Time ( Hina ) t Repeats s i Channel Parent Daughter 0.03 0.00 0.00 9.00 Dwell (Secs) Coll Energy (eV) Cone ( V ) 413.00 119.00 413.00 169.00 413.00 219.00 0.25 0.25 0.25 30 20 30 15 15 15 Function i 2 MX* of 1' Mass Fair ( ESP- J Inter Channel Delay ( Secs ) t Span < Daltons ) i Start Tine ( Mins ) * End Time ( Kins ) * Repeats x Channel Parent Daughter 0.03 0.00 0.00 ' 9.00 ' . ' 1 Dwell (Secs) Coll Energy (eV) Cone ( V ) 463.00 219.00 0.40 20 35 Function s 3 BUOI of 1 Mass Fair ( ESP- ) Inter Channel Delay ( Secs ) s 0.03 Span ( Daltons ) i 0.00 Start Tine ( Mins ) i 0.00 End Tine { Mins ) x Repeats t 9.00 1 Channel Parent Daughter Dwell (Secs) Coll Energy ' (eV) Cone ( V ) 1 513.00 219.00 0.40 20 35 Function < 4 MEM of 1 Mass Fair ( ESP* ) Inter Channel Delay ( Secs ) i Span ( Daltons ) t Start Tine ( Mins ) * End Tine ( Mins ) i Repeats i Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (eV) ' Cone ( V )* 563.00 219.00 0.40 25 30 Function s 5 MIX of 1 Mass Pair ( ESP* ) Inter Channel Delay ( Secs ) i Span ( Daltons ) t Start Tine ( Kins ) x End Tine ( Mins ) i Repeats t * Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (eV) Cone ( V } 13.00 219.00 0.40 20 40 Function s m u m of 1 Mass Pair ( ESP- ) Inter Channel Delay ( Seca ) Span ( Daltons ) t Start Tina ( Mins ) t End T-i-n-e -(--M-i-n-a. ). t Repeats t * Channel Parent Daughter 1 427.00 80.00 0.03 0.00 0.00 9.00 l Dwell (Secs) Coll Energy (eV) 0.40 40 Cone ( V ) 60 " BACK TO MAIN ft FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN Oste: s / i a / y i Analyst: V-t FACTStudy Number ~ T S V - 1 Matrix; Time Points Dilutions? Analyte 1: Pq AA. LOQfir this dataset / g o Re-evaluated brdifferentrange? Analyte 4: M SU2> Acceptable LinearRange: ~~A)Q S T A jQ. ~-- LOQfor this dataset " Re-evaluatedfordifferent range? -- Analyte,5: A Acceptable LinearRange: J2> LOQfir this dataset Re-evahatedfordifferentrange? ^ _____ Analyte 2: j mn m AcceptableLinearRange: -- fOO S^T' LOQfir this dataset _________ -- Re-evaluatedfir differentrange? Analyte 3: -MSS. Acceptable LinearRange: / LOQfir this data set / ? > y 2 > Re-evaluatedfor different range? --r ^ for /W V63 -/Ae LO 1% A^ybyvvwtelL /<Ta.yol. -fouw l (W, 4kc & 3 t i n t s ' * P L'io c c f t s A T ^ O*^-iC^Y*0U-r-K.. ^ 5 T s M 5 & 5 'ftjut-QkiS c>UrC^ A ^ f - V k , / ^ - W i fo b + U - 2 D /;3 s > / ; o ^ 6 x ^ /iy /ff BACK TO MAIN Quantity Cocapound m a r y sport TOX-120 Rat Livar Dilutions Sasple List: B :\IACT-1.FRO\SaaplaDB\051499s Last modified: Mon May 17 14:14:20 1999 Mathod: S:\rACT-l.PRO\MathDB\051499o Last modified: Mon May 17 14:23:38 1999 Job Coda: Printed: Tua May 18 08:22:40 1999 Compound 1: P O M Rana 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 4 051499066 7 051499067 8 051499068 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 17 051499077 18 051499078 19 051499079 20 051499080 21 051499081 22 051499082 23 051499083 24 051499064 25 051499065 26 051499086 27 051499087 28 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114 a^lallat Taxt MeOH Blank MaOK Blank Tox-120, RBL04199-H20Blk-2, 4/19/99 Tox-120, RBl04199-LBlk-2, 4/19/99 Tox-120, 9BL04199-5ppb-2, 4/19/99 Tox-120, 9BL04199-10ppb--2, 4/19/99 Tox-120, 9BL04199-25ppb-2, 4/19/99 Tox-120, BBL04199-50ppb-2, 4/19/99 Tox-120, BBL04199-100ppb-2, 4/19/99 Tox-120, RBL04199-250ppb-2, 4/19/99 Tox-120, BBL04199-500ppb-2, 4/19/99 Tox-120, SBL04199-750ppb-2, 4/19/99 Tox-120, B104199--1000ppb-2, 4/19/99 MeOH Blank Tox-120 23M G2 / D1:2S . Tox-120 24M G2 / Dl:25 ' Tox-120 2SM G2 / 01:25 Tox-120 2K 62 / 01:25 Tox-120, RBL04199--2S0ppb-CCl, 4/19/99 MeOH Blank . Tox-120 33K 63 / 01:25 Tox-120 34M G3 / 01:25 Tox-120 3SM G3 / 01:25 Tox-120 36M G3 / Dl:25 - Tox-120 37M G3 / 01:25 Tox-120 38M <53 / 01:25 Tox-120 39M G3 / 01:25 Tox-120 4QM 03 / 01:25 Tox-120, RBL04199-250ppb-CC3, 4/19/99 MeOH Blank Tox-120 27M 62 / 01:10 Tox-120 29M G2 / Dl:10 Tox-120 3OM G2 / 01:10 Tox-120 31M G2 / 01:10 Tox-120, RBL04199--250ppb-CC4, 4/19/99 MeOH Blank Tox-120 42M G3 / 01:10 Tox-120 43K G3 / 01:10 Tox-120 45M G3 / 01:10 Tox-120 4 M G3 / 01:10 Tox-120 4TM G3 / 01:10 Tox-120, RBlt04199-250ppb-CC5, 4/19/99 MeOH Blank Tox-120, RBL04199--H20BU-2, 4/19/99 Tox-120, XBL04199-LBlk-2, 4/19/99 Tox-120, RBL04199-Sppb-2, 4/19/99 Tox-120, RBI04199-10ppb-2, 4/19/99 Tox-120, m04199-25ppb-2, 4/19/99 Tox-120,' RBL04199--50ppb-2, 4/19/99 Tox-120, RBL04199--100ppb-2, 4/19/99 Tox-120, 9Bl*04199-250ppb-2, 4/19/99 Tox-120, RBLD4199-500ppb-2, 4/19/99 Tox-120, HBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-1000ppb-2, 4/19/99 typ Analyta Analyte Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta Analyta C Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta 0 Analyta Analyta Analyta Analyta Analyta Analyte . QC Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard 8td Cono 5.56 11.10 27.80 55.60 111.00 278.00 ' 556.00 833.00 1111.00 278.00 * 278.00 278.00 278.00 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 R 5.050 5.047 5.050 5.049 5.049 5.036 5.048 5.048 5.050 . 5.048 5.049 5.049 5.049 5.048 5.050 5.050 5.050 5.056 5.049 5.048 5.049 5.049 5.050 5.048 5.049 5.050 5.050 5.048 5.049 5.048 5.049 5.048 5.049 5.052 5.048 5.049 5.051 5.050 5.049 5.050 5.050 5.049 5.048 5.051 Ara 72 132 113 175 406 801 1123 1283 368 592 283 255 464 383 601 481 . 515 503 591 .465 598 485 440 268 281 326 508 263 563 214 177 288 471 81 58 113 39 139 105 249 429 837 1175 1380 Paga 1 Response Cono. H a g s %Dev 71.686 9.88 MM 78 132.374 113.329 175.242 406.110 800.930 1122.779 1282.724 55.49 bb 41.18 bb 87.71 bb 261.22 bb 557.95 bb 799.83 bb 920.04 bbX 10 -26 -21 -6 0 -4 -17 , 368.371 592.299 282.603 254.927 463.685 232.86 bb 401.15 bb 168.40 bb 147.60 bb 304.49 bb 10 \ 382.606 601.330 480.556 515.341 502.581 590.622 464.784 597.567 485.149 243.55 bb 407.94 bb 317.17 bb 343.31 bb 333.72 bb 399.89 bb 305.32 bb 405.11 bb 320.62 bb 1 /\ 1 15 f 439.886 268.097 280.775 325.677 508.13S 286.60 bb 157.50 bb 167.02 bb 200.77 bb 337.90 bb 22 * 262.546 562.997 213.511 177.013 287.691 471.073 153.32 bb 379.13 bb 116.47 bb 89.04 bb 172.22 MM 310.04 bb / 12' 81.083 57.712 112.987 38.519 138.857 104.892 248.672 429.161 837.128 1175.497 1379.791 16.95 MM 0.00 MM 40.92 bbX 636 0.00 MM -100 60.37 bb 117 34.84 bb * -37 142.90 bb : . 29 278.54 bb 0 585.15 bb 5 839.45 bb 1 992.99 MMX -11 . 0M^V . Soup 020199f Raviawad and quantitatad by drb 5/17/99 BACK TO MAIN Quantify Compound Sunaaxy Raport WX-120 Rat Livar Oilutiona Saapla L U t : 8 :\r*CT-l .PRO\Sa^xlaDB\05U9o Laat aodifiad! Mon May 17 14:14:20 1090 Mathod: S:\nCT-l.PRO\MatliDa\OS1499a Laat modiflod: Non May 17 14:23:3 1999 Job Coda: Prlntad: Tua May 1 08:22:40 1999 Coofxnmd 2: M483 Kama 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 S7 051499067 051499068 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 xa17 051499077 051499078 19 051499079 20 051499080 21 051499081 22 051499082 23 051499083 24 051499084 25 051499085 26 051499086 27 051499087 28 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 0S1499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 46 051499108 49 051499109 50 051499110 51 051499111 52 051499112 S3 051499113 54 051499114 a^lallat Taxt MaOH Blank MaOH Blank Tox-120, RBL04199-H2QBlk-2, 4/19/99 TOX-120, RBL04199-LBlk-2, 4/19/99 TOX-120, RBL04199-5ppb-2, 4/19/99 TOX-120, 9BL04199--10ppb-2, 4/19/99 Tox-120, RBL04199-25ppb-2, 4/19/99 Tox-120, RBL04199-50ppb-2, 4/19/99 TOX-120, RBL04199--100ppb-2, 4/19/99 Tox-120, RBL04199--250ppb--2, 4/19/99 Tox-120, RBL04199-500ppb-2, 4/19/99 Tox-120, RBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-1000ppb-2, 4/19/99 MaOH Blank Tox-120 23M 02 / DI:25 Tox-120 24M 02 / DI:25 Tox-120 25M G2 / Dl:25 Tox-120 26M 02 / DI:25 Tox-120, RBL04199-250ppb-CCl, 4/19/99 MaOH Blank /Tox-120 33M 63 /Tox-120 34M 63 /Tox-120 35M G3 /Tox-120 3SM 63 /Tox-120 37M G3 /Tox-120 38M 63 /Tox-120 39H 63 /Tox-120 4OH 63 DI:25 Dl:25 DI:25 DI:25 DI:25 DI:25 DI:25 Dl:25 , Tox-120. RBL04199-250ppb-CC3, 4/19/99 MaOH Blank /Tox-120 27M 62 /Tox-120 29M 62 /Tox-120 SOM 62 /Tox-120 31M 62 . DI: 10 DI:10 Dl:10 DI: 10 Tox-120, RBL04199--2S0ppb-CC4, 4/19/99 MaOH Blank Tox-120 42M G3 / DI: 10 Tox-120 43M 63 / Dl:10 Tox-120 4SM G3 / Di: 10 Tox-120 46M 63 / DI: 10 Tox-120 47M 63 / DI: 10 Tox-120, RBL04199-250ppb-CC5, 4/19/99 MaOH Blank Tox-120, RBL04199-H20Blk-2, 4/19/99 Tox-120, RBL04199-LBlk-2, 4/19/99 Tox-120, RBL04199-5ppb-2, 4/19/99 - Tox-120, RBL04199-l^pb-2, 4/19/99 Tox-120, RBL04199-25ppb-2, 4/19/99 TOX-120, RBL04199-50ppb-2, 4/19/99 Tox-120, RBL04199--100ppb-2, 4/19/99 Tox-120, RBL04199-250ppb-2, 4/19/99 Tox-120, RBL04199-500ppb-2, 4/19/99 Tox-120, RBL04199-750ppb-2, 4/19/99 TOX-120, RBL04199--1000ppb-2, 4/19/99 fypm Analyta Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta ocAnalyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta OC Analyta Analyta Analyta Analyta ocAnalyta Analyta Analyta Analyta Analyta Analyta Analyta OC Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Std Cono 5.56 11.10 27.80 55.60 111.00 278.00 ` 556.00 833.00 1111.00 278.00 5.307 5.306 ` 5.307 5.307 278.00 5.306 5.308 5.308 5.308 5.315 . 5.307 5.306 5.307 278.00 5.308 5.306 5.307 5.307 278.00 5.306 5.307 5.306 5.307 5.306 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 Araa 613 637 509 473 48 49 47 44 44 35 . 44 43 1080 1367 1207 1023 66 83 50 63 55 Aaaponaa Cono. Plaga %Dav 612.731 636.927 509.037 472.806 bbZ tabi bbZ bbl 48.048* *48.581 46.922 43.598 43.676 34.651 44.313 42.794 MMX MMX MMX MMX MMX MMX MMX MMX 1079.684 1367.145 1206.705 1022.573 bbX bbX bbX bbX 66.125 83.086 50.209 63.197 55.155 MMX bbl MMX MMX MMX fi . 9aga 2 Coup 020199, Raviawad and quantitatad by dzb 5/17/99 BACK TO MAIN Quantify Compound guuaary Raport TOX-120 Rat Livar Dilutions ampia U s t i g :\rXCT-l. PRO\SaafilaDB\05149a Last aodifiad! Mon May IT 14:14:20 1099 Mathod: 9:\mCT>l.FRO\MathD8\OS1499a Last aodifiad: Mon May 1? 14:23:3 1999 Job Coda: Frlntad: Tua May 1 09:22:40 1999 ' Faya 3 Coopound 3 : MS13 Kama aaplaliat Tact Atd Cono 8T Area Reepcmee Cono, n a g e %Denr 1 051499061 MeQH Blank Analyte 2 051499062 MaOH Blank Analyte 3 051499063 Tox-120, RBL04199-H20Blk-2, 4/19/99 Analyte 4 051499064 Tox-120, RBL04199-LBlk-2, 4/19/99 Analyte 5 051499065 Tox-120, RBL04199--Sppb-2, 4/19/99 Standard 5.56 6 051499066 Tox-120, RBL04199-10ppb-2, 4/19/99 Standard 11.10 5.466 13 13.419 10.23 MM -8 7 051499067 Tox-120, RBL04199-25ppb-2, 4/19/99 Standard 27.80 5.463 34 34.422 33.18 MK 19 8 051499068 TOX-120, RBL04199-50ppb--2, 4/19/99 Standard 55.60 5.464 49 48.961 49.07 MM -12 9 051499069 Tox-120, RBL04199-100ppb-2, 4/19/99 Standard 111.00 5.464 128 127.672 135.10 bb 22 10 051499070 Tox-120, RBL04199-250ppb-2, 4/19/99 Standard 278.00 5.465 253 253.440 272.54 bb -2 11 051499071 Tox-120; RBL04199-500ppb-2, 4/19/9.9 Standard * 556.00 5.500 467 466.900 505.83 bb -9 12 051499072 Tox-120, RBL04199-750ppb-2, 4/19/99 Standard 833.00 5.464 763 763.341 829.80 bb 0 13 051499073 Tox-120, RBL04199-1000ppb-2, 4/19/99 Standard 1111.00 5.514 764 764.046 830.57 bbX -25 14 051499074 MaOH Blank ] 15 051499075 Tox-120 23M G2 / Dl:25 16 051499076 Tox-120 24M G2 / Dl:25 ' . 17 051499077 Tox-120 2 62 / Dl:25 18 051499078 Tox-120 26M S2 / D1:2S Analyte Analyte Analyte Analyte Analyte 5.515 79 78.528 81.39 MM 5.513 ' 103 103.445 108.62 bb 5.514 59 58.790 59.82 MM 5.514 86 86.441 90.03 bb V> 19 051499079 Tox-120, RBL04199-250ppb-CCl, 4/19/99 QC 278.00 5.515 245 244.847 263.15 bb -5 20 051499080 21 051499081 22 051499082 23 051499083 24 051499084 25 051499085 26 051499086 27 051499087 28 051499088 MaOH Blank Tox-120 33M G3 / D1:2S Tox-120 34M G3 / Dl:25 Tox-120 35M G3 / Dl:25 Tox-120 3EM 63 / Dl:25 Tox-120 3TM 63 / Dl:25 Tox-120 3M 63 / Dl:25 Tox-120 39M 63 / 01:25 Tox-120 40M 63 / Dl:25 Analyte Analyte Analyte Analyte Analyte Analyte Analyte Analyte Analyte 5.514 5.515 5.516 5.516 5.523 5.515 5.514 5.515 90 76 66 . 69 71 49 . 66 64 89.556 93.44 bb 76.379 79.Q4 MM 65.982 67.68 MM 69.198 . 71.19 MM 70.905 73.06 MM 48.849 48.95 MM . 66.234 67.95 MH 64.294 65.83 MM 29 051499089 Tox-120, RBL04199-250ppb-CC3, 4/19/99 QC 278.00 5.514 241 241.294 259.27 bb -7 30 051499090 MaOH Blank Analyte 31 051499091 Tox-120 27M 62 / Dl:10 Analyte 5.514 136 135.719 143.89 bb 32 051499092 Tox-120 29M 62 / 01:10 Analyte 5.514 173 173.108 184.75 bb 33 051499093 Tox-120 30M 62 / 01:10 Analyte 5.514 116 116.489 122.87 bb 34 051499094 Tox-120 31M 62 / 01:10 Analyte 5.515 138 137.808 146.17 bb 35 051499095 Tox-120, RBI,04199-250ppb-CC4, 4/19/99 QC 278.00 5.515 275 274.745 295.83 bb 6 36 051499096 MaOH Blank Analyte 37 051499097 Tox-120 42M 63 / 01:10 Analyte 5.514 111 110.694 116.54 bb 38 051499098 Tox-120 43M 63 / 01:10 Analyte 5.515 121 121.136 127.95 bb 39 051499099 Tox-120 4SM 63 / 01:10 Analyte 5.512 68 67.748 69.61 MM 40 051499100 Tox-120 46M 63 / 01:10 Analyte 5.514 93 93.064 97.27 bb 41 051499101 Tox-120 47M 63 / 01:10 Analyte 5.514 71 71.230 73.41 MM 42 051499102 Tox-120, RBL04199-250ppb-CC5, 4/19/99 QC 278.00 5.515 244 244.465 262.74 bb -5 43 051499103 MaOH Blank Analyte 44 051499104 Tox-120, RBL04199-H20Blk-2, 4/19/99 Analyte 45 051499105 Tox-120, RBL04199-LBlk-2, 4/19/99 Analyte 46 051499106 Tox-120, RBL04199-5ppb-2, 4/19/99 Standard 5.56 47 051499107 Tox-120, RBL04199-10ppb-2, 4/19/99 Standard 11.10 5.516 15 14.743 11.68 MM 5 48 051499108 Tox-120, RBL04199--25ppb-2, 4/19/99 Standard 27.80 5.515 23 23.309 21.04 MM -24 49 051499109 Tox-120,- RBI*04199-50ppb-2, 4/19/99 Standard 55.60 5.513 50 50.399 50.65 MM * -9 50 051499110 Tox-120, RBL04199-100ppb-2, 4/19/99 Standard 111.00 5.514 116 116.114 122.46 b b 3 . 10 51 051499111 Tox-120, RBL04199-250ppb-2, 4/19/99 Standard 278.00 5.516 262 262.095 282.00 bb 1 52 051499112 Tox-120, RBL04199-500ppb-2, 4/19/99 Standard 556.00 5.515 547 546.872 593.23 bb 7 S3 051499113 TOX-120, RBI04199-750ppb-2, 4/19/99 Standard 833.00 5.513 762 761.863 828.19 bb -1 54 051499114 Tox-120, RBL04199-1000ppb-2, 4/19/99 Standard 1111.00 5.516 773 772.968 840.32 MMX -24 Soup 020199, Roviwad and quantitatad by drb 3/17/99 BACK TO MAIN Quantify Compound Sinnary Xaport TOX-120 Rat Liver Dilutions lampi. H a t ; 8; \rACT-l. PRO\SaaplaOB\0514Sa Last modifiad; Mon May 17 14:14:20 I M S Mathed: S:\IACT-l.PR0\MatliDB\051499o Last modifiad: Mon May 17 14:23:38 19 Job Coda: Prlntad: Tua May IB 08:22:40 199 Compound 4: MS63 Han 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 7 051499067 e 051499068 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 17 051499077 18 051499078 19 051499079 20 0514990B0 21 051499081 22 051499082 23 051499083 24 051499084 25 051499085 25 051499086 27 051499087 20 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114 SanplaXist tact MaOH Blank MaOH Blank Tox-120, RBL04199-H20Blk-2, 4/19/9 Tox-120, *BL0419-LBlk-2, 4/19/99 Tox-120, BBL04199-5ppb-2, 4/19/9 Tox-120, RBL04199-10ppb-2, 4/19/99 Tox-120, BBL04199-25ppb-2, 4/19/99 TOX-120, RBL04199-50ppb-2, 4/19/99 TOX-120, HBL04199--100ppb-2, 4/19/9 TOX-120, R8L04199-250ppb-2, 4/19/99 TOX-120, RBL04199-500ppb-2, 4/19/99 TOX-120, RBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-1000ppb-2, 4/19/99 MaOH Blank Tox-120 23M G2 / Dl:25 . Tox-120 24M 62 / Dl:25 - Tox-120 2SM 62 / Dl:25 Tox-120 204 G2 / 01:25 Tox-120, RBL04199-250ppb-CCl, 4/19/9 MaOH Blank . Tox-120 33M G3 / D1:2S Tox-120 34M 63 / Dl:25 Tox-120 3SM G3 / D1:2S Tox-120 304 6 3 / 0 1 : 2 5 Tox-120 37M 63 / Dl:25 Tox-120 38M 63 / D1:2S Tox-120 39M 63 / Dl:25 Tox-120 40M 63 / Dl:25 Tox-120, RBL04199-250ppb-CC3, 4/19/9 MaOH Blank Tox-120 27M 62 / 01:10' Tox-120 29M 62 / Dl:10 Tox-120 30M 62 / 01:10 Tox-120 31M 62 / 01:10 Tox-120, RBL04199-250ppb-CC4, 4/19/99 MaOH Blank Tox-120 42M 63 / 01:10 Tox-120 43M 63 / 01:10 Tox-120 4SM 63 / 01:10 TOX-120 404 63 / 01:10 Tox-120 47M 63 / 01:10 Tox-120, RBL04199-250ppb-CC5, 4/19/99 MaOH Blank Tox-120, RBL04199-B20Blk-2, 4/19/99 Tox-120, RBIt04199-LBlk-2, 4/19/99 Tox-120, ABL04199-5ppb-2, 4/19/99 Tox-120, RBL04199-10ppb-2, 4/19/99 Tox-120, RBL04199-25ppb-2, 4/19/99 Tox-120, RBL04199-50ppb-2, 4/19/99 Tox-120', RBL04199-100ppb-2, 4/19/99 TOX-120, RBL04199--2S0ppb-2, 4/19/99 Tox-120, RBL04199-500ppb-2, 4/19/99 Tox-120, RBL04199-750ppb-2, 4/19/99 Tox-120, BBL04199-1000ppb-2, 4/19/99 Typa Analyta Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyte Analyte QC Analyta Analyta Analyta Analyta Analyte QC Analyta Analyta Analyte Analyte Analyta Analyta QC Analyta Analyte Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard 8td Cono XT 5.56 11.10 27.80 55.60 111.00 278.00 ` 556.00 833.00 1111.00 278.00 5.671 ` 5.671 5.671 278.00 278.00 5.672 5.672 5.671 5.672 278.00 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 Ama 44 15 21 . 45 70 45 47 JtACpon Cono, flags 44.162 14.826 20.809 Ma Ma Ma 45.418 70.127 44.525 47.121 Ma Ma Ma Ma Taya 4 Soup 020199, Raviavad and quantitatad by drb 5/17/99 ? ! ro > o 7 o > Soup 020X99, Raviowad and quand tatad by drb 5/17/99 BACK TO MAIN Quantify Compound Sunnary Report TOX-120 Rat Liver Dilution Sample List: 8:\FACT-1.PRO\SasplaDB\051499q Last modified: Mon May 17 14:14:20 1999 Mathod: S:\FACT-l.PRO\MethDB\051499o Last modified: Mon May 17 14:23:38 1999 Job Coda: Printad: Tua May 18 08:22:40 1999 Compound 6: ZS # Mama 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 7 051499067 8 051499060 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 17 051499077 10 051499078 19 051499079 20 051499000 21 051499081 22 051499082 23 051499083 24 051499084 25 051499085 26 051499086 27 051499087 28 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 30 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114 8 mapilist Tuet MeOH Blank MaOH Blank Tox-120, HBL04199--H20Blk-2, 4/19/99 Tox-120, RBL04199-lBlk-2, 4/19/99 Tox-120, RBL04199-5ppb-2, 4/19/99 Tox-120, RBIi04199-10ppb-2, 4/19/99 Tox-120, RBt,04199-2Sppb-2, 4/19/99 Tox-120, BBL04199-50ppb-2, 4/19/99 Tox-120, BBL04199-100ppb-2, 4/19/99 Tox-120, RBL04199-250ppb-2, 4/19/99 Tox-120, RBL04199-500ppb-2, 4/19/99 Tox-120, BBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-1000ppb-2, 4/19/99 MeOH Blank Tox-120 23K 62 / 01:25 Tox-120 24M 62 / 01:25 . ' Tox-120 25M 62 / 01:25 Tox-120 26M 62 / 01:25 Tox-120, RBX04199-250ppb-CCl, 4/19/99 MeOH Blank Tox-120 33M 63 / 01:25 Tox-120 34M 63 / 01:25 Tox-120 3SM 63 / Dl:25 Tox-120 34M 63 / 01:25 Tox-120 37M 63 / 01:25 Tox-120 38M 63 / 01:25 Tox-120 39M 63 / 01:25 Tox-120 40M 63 / 01:25 Tox-120, RBL04199-250ppb-CC3, 4/19/99 MeOH Blank Tox-120 27M 62 / Dl:10 Tox-120 29M 62 / 01:10 Tox-120 30M 62 / 01:10 Tox-120 31M 62 / Dl:10 Tox-120, BBL04199-250ppb-CC4, 4/19/99 MeOH Blank Tox-120 42M 63 / 01:10 Tox-120 43M 63 / 01:10 TOX-120 45M 63 / 01:10 Tox-120 46M 63 / Dl:10 Tox-120 47M 63 / 01:10 Tox-120, RBL04199-250ppb-CC5, 4/19/99 MaOH Blank Tox-120, HBL04199-H2QBlk-2, 4/19/99 Tox-120, RBL04199-LBlk-2, 4/19/99 Tox-120, RBL04199-5ppb-2, 4/19/99 Tox-120, RBL04199-10ppb-2, 4/19/99 Tox-120, RBL04199--25ppb-2, 4/19/99 Tox-120, RBL04199--50ppb-2, 4/19/99 Tox-120, RBL04199--100ppb-2, 4/19/99 Tox-120, R B U M l O ^ S O p p b ^ , 4/19/99 Tox-120, RBL04199-500ppb-2, 4/19/99 Tox-120, RBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-- 1000ppb-2, 4/19/99 Typa Analyte Analyte Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyte Analyta QC Analyte Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Std Cono RT 5.036 5.035 1.00 5.036 1.00 5.036 1.00 5.033 1.00 5.036 1.00 5.034 1.00 5.035 . 1.00 5.023 1.00 5.034 1.00 5.034 1.00 5.036 5.034 * 5.035 5.035 5.035 1.00 5.034 5.036 5.036 5.036 5.042 5.035 5.034 5.035 5.035 1.00 5.037 5.034 5.035 5.036 5.036 1.00 5.034 5.035 5.034 5.035 5.034 5.035 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 5.038 5.034 5.035 5.037 5.036 5.035 5.036 5.036 5.035 5.034 5.037 Area Response Cono. Flags %Dav 2407 2496 2699 2722 2446 2280 2566 2352 . 2408 2309 2302 2406.723 2496.454 2699.373 2722.382 2446.267 2280.384 2565.513 2351.564 2408.171 2308.686 2301.694 81 109 74 70 2336 81.237 109.041 74.247 69.750 2336.227 170 81 71 95 84 83 . 91 73 2253 169.517 80.550 71.387 94.601 04.019 82.882 96.582 72.744 2253.416 163 198 184 179 2464 163.472 198.362 183.888 178.796 2464.372 214 175 164 170 161 2414 213.740 174.571 163.908 169.648 160.737 2413.697 2416 2396 2542 2751 2500 2337 2586 2464 2333 2355 2457 2415.683 2395.952 2541.736 2751.209 2500.397 2336.569 2585.568 2463.607 2333.406 2355.073 2457.479 0.98 bb 1.01 bb 1.09 bb 1.10 bb 0.99 bb 0.92 bb 1.04 bb 0.95 bb 0.98 bb 0.94 bb 0.93 bb -2 1 9 10 -1 -8 4 -5 -2 -6 -7 0.03 bb 0.04 bb 0.03 Ml 0.03 MM 0.95 bb -97 -96 -97 -97 -5 0.07 bb 0.03 bb 0.03 MM 0.04 bb 0.03 bb 0.03 bb 0.04 bb 0.03 MM 0.91 bb -93 -97 -97 -96 -97 -97 -96 -97 -9 0.07 bb 0.08 bb 0.07 bb 0.07 bb 1.00 bb -93 -92 -93 -93 0 0.09 bb 0.07 bb 0.07 bb 0.07 bb 0.07 bb 0.98 bb -91 -93 -93 -93 -93 -2 0.98 bb -2 0.97 bb -3 1.03 bb 3 1.12 bb 12 1.01 bb 1 0.95 bb * -5 1.05 bb ?. 5 1.00 bb * 0 0.95 bb -5 0.95 bb -5 1.00 MM 0 Paga 6 Soup 020199, Reviewed and quantitatad by drb 5/17/99 Quantlfy Calibrati Raport TOX-120 Hat U v w D ilu tlo n a C a lib ra t i e n i a :\n tC T -l. FM>\CarvaSB\051488e L aat a o d if la d : Tea May 18 08:21:03 1 P z ln tad : Tua Miy 18 08:22:33 1888 ' BACK TO MAIN * l p>* 1 Boup 020188, Ravlavad and quantitatad by dxb 5/17/88 Quantify Calibration Raport TOX-120 Rat Livar Dllutlona Calibratlons R:\mcT-l.FR0\CurvaDR\051499a laat nodiriad: Ina May IR 0R:21:03 1999 Printad: Tua May IR 0Ri22:S3 1999 ' BACK TO MAIN ' PS 2 .' ' Roup 020199, Ravlawad and quantltatad by drb 5/17/99 Quantify Calibration Itaport TOX-120 Hat Llvar Dilution Calibration: S:\TACT-l.PR0\CurvaDB\0S1499a Last nodifiad: Tua Hay It 08:21:03 1999 Printad: Tua May 18 08:22:53 1999 BACK TO MAIN Soup 02019, Baviawad and quantitatad by drb 5/17/99 Ouantify Calibratimi tepect TCK-120 Rat Livar Dilutions Calibratimi: a:\nkCT-1.9RO\CurvuD8\051499a Laat Bodifiad: Tua May 11 08:21:03 1998 Printad: tua May 19 08:22:83 1999 BACK TO MAIN Paga 4 Soup 020199, Rawiaaad and quantitatad by drb 5/17/99 1 Quantify Calibration Raport TOX-120 Rat Llvar Dilutlona Calibrition: I:\r*cr-l.rao\CurvDB\0314990 Lait aodiflad: Tua May la 08:21:03 1900 Frlntad: Tua May la 08:22:53 1999 BACK TO MAIN * Paga 5 8oup 020199, Ravlawad and quantitatad by dzb 5/17/99 Quantify Calibrati! Raport TOX-120 Rat U v a r Dilati Calibrati!! I:\riCT-i.PJO\CurvaDB\051499o Laat aodifiad: Tua May la 08:21:03 1 0 M Frintad: Tua May lt 08:22:53 1099 BACK TO MAIN ' * < Method Report Method file: Lest modified: Compound: Printed: S:\rACT-l.PRO\MethDB\05H99c.mdb Mon May 17 14:23:38 19990 1: P C M Tue May 18 08:41:45 1999 Compound Heme: Internal Ref.: Quantify Trace: Cone, of Standards: PCM [Hone] TIC Cone. A User RT Value: User Peak Factor: Integrate Window Factor: 1.000000, Disabled 1.000000 2 .0 0 0 Peak Location Retention Time (mina) : Relative Retention' Time: Time Window (mine): 4.998, enabled 0.000, Disabled 0.400 Peak Selection: Nearest Response Response Type: j Response: Esternai Areas Calibration Curves I Polynomial Type: ' Llneer I Point of Origin: Exclude 1 Fit weighting: 1/X I Axis Transformation: None Concentration Units: Noise Peak-to-peak amplitude: 140 Smooth . Smoothing: Window size (scans): Number of smooths: Smoothing method: Disabled 3 2 Baselines Join valley threshold: Reduce peak tailing: Raise baseline maximum: 20.00 100.00 0.00 Peak Separation Draw vertical threshold: Detect shoulders: Shoulder threshold: 10.00 Disabled 40.00 Response Threshold Relative height: Absolute height: Relative area: Absolute area: 1.50, Disabled O.OO, Disabled 2.00, Disabled 0.00, Enabled Spty O l D l W .`x BACK TO MAIN Page 1 Method Report Method file: Lest modified: Compound: Printed: S:\FXCT-1 .PRO\MethDB\051499c.mdb Mon Mey 17 14:23:38 19990 2: H463 Tue Mey 18 08:41:45 1999 Compound Heme: Internal Ref.: Quantify Trees: Cone, of Standards: M463 [Nona] TIC Cone. A User RF Value: User Peek rector: Integrate Window Factor: 1.000000, Disabled 0.000000 2.000 Peek Location Retention Time (mins): Relative Retention Time: Time Window (mins): 5.408, Enabled 0.000, Disabled 0.551 Puk S.laction: Neareat Response Kaapona. Typ: Kaapona.: ' Extarnal . Araas . - Calibration C o m a Polynomial Typa: Point of Origin: Fit Knighting: Axis Transformation: Linear Exclude 1/X None Concentration Units: Noise Psak-to-paak aapiituda: 140 Smooth Smoothing: Window size (scans): Number of smooths: Smoothing method: - Disabled *3 2 Baselines Join valley threshold: Reduce peek tailing: Raiae baseline maximum: 20.00 100.00 0.00 Peak Separation Draw vertical threshold: Detect shoulders: , Shoulder threshold: 10.00 Disabled 40.00 * Aasoonsa Threshold Relative height: absolute height* Relative area: Absolute area: 1.50, Disabled : O.OO, Disabled 2.00, Disabled 80.00, Enabled (AtSn/xp n T lW Offaojlj BACK TO MAIN Pag* 2 H.thod Report Method file! Lest aodifltd: Compound: Printed: S:\raCT-l.MU>\MethD8\051499c.ndb Mon May 17 14:23:3 19990 3: MS13 Tue Hey IS 0S:41:4S 1999 Compound Mame: Internal Rei.: Quantify Trace: Cone, of Standards: M513 [None] TIC Cone. A User XT Valu! User Peak Factor! Integrato Window factor! 1.000000, Disabled 1.000000 2 .0 0 0 Peak Location Retention Tina (nina): Relative Retention Times Time Window (mine) : 5.538, Enabled 0.000, Disabled 0.325 Peak Selection: Nearest Reaponae Reaponae Type: Reaponae: External Areas Calibration Curves Polynomial Type: Point of Origin: Tit Weighting: Axis Transformation: Linear Exclude 1/X None Concentration Units: * Noise Peak-to-peak amplitude: 140 Smooth . Smoothing: Window size (scans): Number of smooths: Smoothing method: Disabled 3 *2 Baselines Join valley threshold: Reduce peak tailing: Raise baseline maximum: 20.00 100.00 0.00 Peak Separation Draw vertical threshold: Detect shoulders: Shoulder threshold: x* 10.00 Disabled 40.00 Response Threshold Relative height: Absolute height: Relative area: Absolute treat 1.50, Disabled 0.00, D is a b le d 2.00, Disabled 60.00, Enabled U c v/loljf BACK TO MAIN Page 3 Method Report Method file: Last modified: Compound: Printed: S:\FACT-1. PRO\MethD8\051499c.i Mon May 17 14:23:38 1999Q 4: MS63 Tue Kay 18 08:41:46 1999 Coapound Name: Internal Ref.: Quantify Trace: Cone, of Standards: User AT Value: User Peak Factor: Integrate Window rector: Peak Location Retention Tijne (nine): Relative Retention Tina: Tine Window (mins): P u k S.l.ction: ' Raisons. Response Type: Response: Calibration Cnrni Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation: Concentration Onitii Wbise ioak-to-paak aaplltuda: Smooth Smoothing: Window size (scans): Number of smooths: Smoothing method: Baselines Join valley threshold: Reduce peak tailing: Raise baseline maximum: Peak Separation Draw vertical threshold: Detact shoulders: Shoulder threshold: - Response Threshold Relative height: Absolute height:. Relative area: Absolute area: a m a oeooe oooooo ooo HHN M563 (Nona) TIC Cone. A 5.349, Enabled 0.000, Disabled 0.511 Raarast External Areas Linear Exclude 1/X Hone 140 Disabled 3 2 20.00 100.00 0.00 10.00 Disabled 40.00 1.50, Disabled > 0.00, Disabled 2.00, Disabled 0.00, Enabled I asliefa iAt OX,' M f f BACK TO MAIN Paga 4 Method Report Method file: haet modified: S:\nCT-l. PRO\MethDB\OS1499c.mdb Mon May 17 14:23:3 19990 Coapound: S: M613 Printed: Tua May 1 08:41:46 1999 Compound Ki m : Internal Ref.: Quantify Trace: Cone, of Standardss H613 [Nona] TIC Cone. A SbUj) AlftH9 tAL Usar RT Valus* Usar Pask factor: Intagrata Window Factor: 1.000000, Disabled 1.000000 2 .0 0 0 Peak Location Ratantion Tima (mins) * Relative Retention Tima: Tima Window (mins) * 5.853, Enabled 0.000, Disabled 0.275 Peak Selection* Nearest Response Response Type* Response* External Areas Calibration Curses Polynomial Type* Point of Origin* Fit Weighting: Axis Transformation: Linear Exclude l/X . None Concentration Units: Noise Peak-to-peak amplitude: 140 Smooth Smoothing: Window sire (scans): Number of smooths: Smoothing method: Disabled 3 2 Baselines Join valley threshold: Reduce peak tailing: Raise baseline maximum: 20.00 100.00 0.00 Peak Separation Draw vertical threshold* Detect shoulders: Shoulder threshold: .' 10.00 Disabled 40.00 Response Threshold Relative height: Absolute height: Relative area: Absoluta area: 1.50, Disabled 0.00, Disabled 2.00, Disabled 80.00, Enabled BACK TO MAIN Pag 5 Method Report Method file: Last Modified: Compound: Printed: S:\IACT-1.PRO\MathDB\051499c.i Hon May 17 14:23:38 19990 6: ZS Tue May 18 08:41:46 1999 Compound Hama: Internal Ref.: Quantify Trace: Cone, of Standards: ZS [None] TIC Fixed User RF Value: 1.000000, Disabled User Peak Factor: 1.000000 l Integrate Window Factor: 2 .0 0 0 Peak Location Retention Time [asina): Relative Retention Time: Time Window (mins) : 5.034, Enabled 0.000, Disabled 0.200 Peak Selection: Nearest KMDOtkl. ] Response Typ.l lupanni External Areas . Calibration Curves Polynonial Type: Point of Origini Fit Weighting: Axis Transformation: Linear Exclude 1/X Mona Concentration Units: Noise Peak-to-peak amplitude: 140 ' Smooth I Smoothing: Window size (scans): Number of smooths: Smoothing method: Disabled 3 2 ` Baselines Join valley threshold: Reduce peak tailing: Raisa baseline maximum: 20.00 100.00 0.00 1 Peak Separation Draw vertical threshold: Detect shoulders: Shoulder threshold: 0 10.00 Disabled 40.00 Response Threshold ! Relative height: Absolute height: Relative area: Absolute area: ! 1.50, Disabled , 0.00, Disabled 2.00, Disabled '80.00, Enabled I blolM M e of/zbfa l BACK TO MAIN Page 6 I 1 J ] 1 ] ] ' 1 I I SOUP 020199: FA CT E quipm ent Log 99014 Quattron, #6414E DATE: 05/21/99 . ANALYST: drb FACT Method: ETS-8-S.0 . DESCRIPTION: Dilutions/Telmer Injectionvolume: IO11T. Cycletime:9 min. Electrode:Z-Sprav Mobile Phase: fA12.0m M NH4CH3C02 /BlMeOH TN-A-orstndrdnumber /Al 99011-6-9 B1TN-A-2540 Prepared(date/by): /Al04/28/99drb fBl05/21/99kih Expires: A110/99 Storeatroomtemp? Jy| or N Other: fBl 10/99 . ' Guard column: None Partnumber: na ' Lot number:na Analyticalcnhimn: KevstoneBetasil Size:2 x50m m Tvne:C18 Partnumber: 055-701-2 Lotnumber:P7L04 Sphere size:5 um Serialnumber:0481175Z Analyticalparameters: DrvineeasfL/hrl:350 ESInebulizinggas(L/hr)l?,,, SheathgasfL/hrl0 LC Flowrate:300nT/min. Backpressure:55bar HPLC solventgradient:9min-2 Tintefmin.l n 0.QQ_______ 2)_LQQ________ 314.50_______ 416.50_______ 51 7.00_______ %B 400 MSL 95.0 95.0 40.0_ BACK TO MAIN Tuning Method Report Method: C s\MASSLYNX\FACT-1. PR0\ACQUDB\PF0S2 Printed: Fri May 21 16:28:16 1999 BACK TO MAIN Page 1 MtMrn MS MS2 SOURCE ( ESP- ) Capillary Cone . Extractor RF Lens . Source Block Temp. Desolvation Temp. Set Rdbk 3.01 40 5 0.2 150 250 3.01 -46 -8 153 254 Mit, .-.1^5 MSI LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier Pressures ' Analyser Vacuum Gas Cell Rdbk 2.4e-4 4.8e-3 .V MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ran Lens 8 Lens 9 Multiplier Set Rdbk 14.0 14.0 2-0 0.0 ' ' 53 650 Set Rdbk 15.0 15.0 45 2.0 0.0 40 0 650 43 39 43 Masslynx Sample List Sample List: C:\MASSLYNX\FACT-1 .PRO\Sam pleDB\052199b.SPL Printed: Fri May 21 16:28:031999 BACK TO MAIN Page 1 H ie Name File Text 1 052199068 MeOH Blank 2 052199069 MeOH Blank 3 052199070 Tox-120, RBL04199-H20Blk-1,4 /1 9 /9 9 4 052199071 Tox-120, RBL04199-LBIk-1,4/19/99 5 052199072 Tox-120, RBL04199-5ppb-1,4 /1 9 /9 9 6 052199073 Tox-120, RBL04199-1 Oppb-1,4 /1 9 /9 9 7 052199074 ' Tox-120, RBL04199-25ppb-1,4/19/99 8 052199075 Tox-120, RBL04199-50ppb-1,4 /1 9 /9 9 9 052199076 Tox-120, RBL04199-100ppb-1,4 /1 9 /9 9 10 052199077 Tox-120, RBLQ4199-250ppb-1,4 /1 9 /9 9 11 052199078 Tox-120, RBL04199-500ppb-1,4 /1 9 /9 9 12 052199079 Tox-120, RBL04199-750ppb-1,4 /1 9 /9 9 13 052199080 Tox-120, RBLQ4199-1000ppb-1,4 /1 9 /9 9 14 052199081 MeOH Blank 15 052199082 Tox-120 4 9 M G 4 /D 1 :1 0 16 052199083 Tox-120 50M G 4 /D 1 :1 0 17 052199084 Tox-120 51M G4 / D1:10 18 052199085 Tox-120 5 2 M G 4 /D 1 :1 0 19 052199086 Tox-120, RBL04199-250ppb-CC1,4 /1 9 /9 9 20 05219987 MeOH Blank 21 052199088 Tox-120 5 3 M G 4 /D 1 :1 0 22 052199089 Tox-120 5 4 M G 4 /D 1 :1 0 23 052199090 Tox-120 5 5 M G 4 /D 1 :1 0 24 052199091 Tox-120 56M G 4 /D 1 :1 0 25 052199092 Tox-120, RBL04199-250ppb-CC3,4/19/99 26 052199093 MeOH Blank ' 27 052199094 Tox-120 6 5 M G 5 /D 1 :5 0 0 28 052199095 Tox-120 6 6 M G 5 /D 1 :5 0 0 29 052199096 Tox-120 6 7 M G 5 /D 1 :5 0 0 30 052199097 Tox-120 68M G 5 /D 1 :5 0 0 31 052199098 Tox-120, RBL04199-250ppb-CC11 ,4 /1 9 /9 9 32 052199099 MeOH Blank 33 052199100 Tox-120 69M G 5 /D 1 :5 0 0 34 052199101 Tox-120 7 0 M G 5 /D 1 :5 0 0 35 052199102 36 052199103 Tox-120 71M G5 / D1:500 Tox-120 72M G5 / D1:500 37 052199104 TOX-120, RBL04199-250ppb-CC1, 4/19/99 38 052199105 MeOH Blank 39 052199106 Tox-120 7 3 M G 5 /D 1 :2 5 0 40 052199107 Tox-120 7 4 M G 5 /D 1 2 5 0 41 052199108 Tox-120 7 5 M G 5 /D 1 :2 5 0 42 052199109 Tox-120, RBL04199-250ppb-CC11 ,4 /1 9 /9 9 43 052199110 MeOH Blank 44 052199111 Tox-120 7 7 M G 5 /D 1 5 5 0 45 05219912 Tox-120 78M G 5/D 1 50 46 052199113 Tox-120 7 9 M G 5 /D 1 :2 5 0 47 052199114 Tox-120, RBL04199-250ppb-CC1,4 /1 9 /9 9 48 052199115 MeOH Blank 49 052199116 Tox-120, RBL04199-H2OBIk-1,4/19/99 50 052199117 Tox-120, RBL04199-LBlk-1,4/19/99 51 052199118 Tox-120, RBL04199-5ppb-1,4 /1 9 /9 9 52 052199119 Tox-120, RBL04199-10ppb-1,4/19/99 53 052199120 Tox-120, RBL04199-25ppb-1,4/19/99 54 052199121 Tox-120, RBL04199-50ppb-1,4 /1 9 /9 9 55 052199122 Tox-120, RBL04199-100ppb-1,4/19/99 56 052199123 Tox-120, RBL04199-250ppb-1, 4/19/99 57 052199124 Tox-120, RBL04199-500ppb-1, 4/19/99 58 052199125 Tok-120, RBL04199-750ppb-1,4 /1 9 /9 9 59 052199126 Tox-120, RBL04199-1000ppb-1,4/19/99 . . M S H ie T E -P F 2 T E U -P F 2 TELI-PF2 T E L I-P F 2 T E U -P F 2 TELI-PF2 T E U -P F 2 T E U -P F 2 T E U -P F 2 . TEU-PF2 T E U -P F 2 T E U -P F 2 T E -P F 2 T E U -P F 2 T E -P F 2 T E U -P F 2 T E U -P F 2 TELI-PF2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 TELI-PF2 TELI-PF2 TELI-PF2 T E U -P F 2 T E U -P F 2 TELI-PF2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 . TEU-PF2 TELI-PF2 T E U -P F 2 Inlet H ie Bottle lume Sam ple T 9min-2 9m in-2 9m in-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9mln-2 9mIn-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9mln-2 9min-2 9min-2 9min-2 9min-2 9m in-2 9min-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m In-2 9m ln-2 9m ln-2 9m in-2 9m in-2 9min-2 9m in-2 9m in-2 9mln-2 9mln-2 9min-2 9min-2 9mln-2 9min-2 9mln-2 9min-2 9mln-2 9m!n-2 9min-2 9m ln-2 9min-2 9mIn-2 9min-2 9min-2 9min-2 91 91 79 80 81 82 83 84 85 86 87 88 89 92 57 58 59 60 90 96 61 62 63 64 99 92 65 66 67 68 100 93 - 69 70 71 72 90 94 73 74 75 100 95 76 77 78 90 96 79 80 81 82 83 84 85 86 87 88 89 . 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte to Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Standar 10 Standar 10 Standai 10 Standai 10 Stanta' 10 Stanta 10 Stanta 10 Stanta 10 Stanta Searmisa <*thod sport Msthodl ct\ O M B L r X X \ T C T - l .WOUCQOB\TKtI-Pfa U i t Modifiedz Fri May 21 15:13:53 199$ Printedt Fri May 21 16:28*25 1999 l o ln at Stliy ( ina ) s 0.00 Fuseti,oa < 1 MRM of a Masi Faira ( ICT- 1 Zntar Osannai Dalay ( Sacs ) t Spasi ( Daltona ) t Start Tla* ( Miai ) s End Tina ( Mina ) t . Rapaata s Osannai Parant Daughter 0.03 0.00 0.00 9.00 1 (S.ca) Coll Energy (V) Cosse ( V ) 1 399.00 99.00 0.25 2 399.00 130.00 0.25 45 45 60 60 HH? HH mastics i 2 m et 2 K u i valsa ( Uf- ) Zntar Osannai Dalay ( Seca ) i 0.03 Span ( Daltona ) i 0.00 Start Tino ( Mina J s 0.00 End Tina ( Mina ) s 9.00 Rapaata s 1 Osannai Parant Daughter (S.cc) Coll Energy (V) . Cono ( V ) 1 399.00 99.00 0.25 2 299.00 130.0^1 0.25 45 45 60 60 Funetioa i 3 MRM of 1 Masa Fair ( ESP- ) Zntar Osannai Dalay ( Sacs ) t 0.03 Span ( Daltona J i 0.00 Start Tina ( Mina ) : 0.00 End Tina < Mina ) s 9.00 Rapaata * 1 Osannai Parant Daughter Duali (Sacs) Coll Energy {.V) 1 427.00 80.00 0.25 40 60 U8O (V ) BACK TO MAIN ' FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN Dte: 5 ~ M I S S ______ _ n c jHu.173u.no i<toto' m ii/tt/n Pi x sssssssssssssaatcessssasssSsssiiBSSSsaBSgscsKGsccss illillllli aas sasa saaaa gassa *SS U S VN S N N N S S 333 233 2223 22 m i m mil i- iIPI*PIp fl ; ; ( i t- ! Ijf s Sgi , ! if!! f I Is f l u a ca lllllit t b m ****** ***** *****lllflftff**** kCSwSt^h uwihMI hMw tmj 3m si ibe m m ebhiooooooopooonQaOnu oe oo ` UUM*JK ; mm sSSSSses ssSs SasS s l i s t S53HS 2 . 2 lim 3SS mm gSmSSS MfItO_ HI AiIHHUHMM m m m m i' i m s s m sm s' sssss s s m0 m* >tS 3 . gssssm l*- HN ? c o A !c ro orO > --r> 7 H O s > o* < kf I l t i l i tm *4 i mnnmtn*fmin4 n*mn on>vthl Il I I I I I i fi aaaaaaaaaaa aaaaa aaaaa a c a aaaaaaaaaaa SonfNoiNona<Hi<4nfro'ffHiAfNiOofr>wi moh*At*f*MlnHr iuitaninnin<fniA ** hfi > in*in*ininirtn riHHHHHHOOOH 4 H4H*4HHrlHHIO 38 e ooooooo .un im iim umimi mi li mi fi 5n 8m 1! a4 s8 a19sn 3835 ss 5S6 RKf#lMRn sm S 3 3 *n , llt I 1un i I-ll1t4 -W34 a? fin 31iU ! uhi fi* *> fsftm i AMA tfAiAsAsOs IIsINn fi A A W V A A f i A H H N SfififiH H H H S V fifififiK S N S N fi **>***** H N '.A A A H H H H S H**.HN.S-0SN <f 0s0a0s0s&i se seseseJ1 Sofia? asas? fissa? asas? sas? sas - - - * g o Sof i oi nemo HHHHC1 HHHN HHHN 3 JA H N lA H N A I'H 5*i i*i* **SiS5** > V.X vfi 4*, *9s s * k s \ ~ *-S-Nf-i -N-> * t 33333 33333 88383 38883 3383 8883 33000000000 ^-Bxl.l.l..i.l a l_l l i g xcsga sal ^dsnsnsnsnl ^dswsgwsl ^gsisr-arsr*l ^qsrs~srl-x qrsrapg*l ^gl s i l l l l i g l a <i M l l<O ONONONNO (OiONNq ONNOnHBOiiO NOrOONnH NONOnOn(O iOrHOHNOrOiONOriHnOiOlNO MBNOnHnBO(#iOnnO iH i0B0n0nn0 n* n4 N0n0N0t0i0nN0 n0 <0 i0n0N0n H H H H H H H H H H H h H H H H H rH H H H H H H H H H H H H H H H H H riH H H H H H H H H H H H W H lUUUUUnUUUUUMUUIUUiUUI S S N n *<n*<r,->(rt>*# *>0H<1',* in,*l'l*A e H N n * in,*r*M A e H N n * ,n'r'"*>eH,1n* n<tr'"akO,4NnviA* ---- a o o o o o a a a o o o o o a a a o a v o AAAAAAAAAAAAAAAAAAAAA A A A A A A A A A A A A A A A A A A A A f i . . ... . ...................................................H HH H HH H rlH H riH H HH H HH H HH rlHH H #'................................................................................................................................ iN N N N N r iN iin N n r in c ir iN N N n n n N N n N N N N N N n iin n N r ir if in n f t r in n n n N r ir ir ir iN N t ir in n r in foifoifiafiofifoifoiifolfiefienonfoifoinonofiAe eAeinofiefionno Mo onofiionefiioneifloinofieinoino foiinoieAofionfeime eineinafoinoifolfiainoine ionoinoinofiomafoiMomo ein. i> i M h A O H N n fiiii < ih fiA e H n n tn h fiA O H N n in i*rfiA 4 iin n n N riN n n n n fii>in n n n <<f < n n fifiM in fifi en oaoifli ouaacic*e*4 by ral printfid by kjh 05/34/# Machad Report Method file* Laat nodifled* Compoun d * Printed* ' Ci\MA36LYNX\PACT-l.PRO\MethDS\052199b.ndb ' Moa Kay 24 07*99*4 1999 1 f n t a Mon Kay 24 00*14*21 1999 _____________ :____ S k u o Incarnai Ref.t Quantify Traca* Cooa. of Standards* Osar RP Valuat Usar Peek Pactar* Zntagrae# Window Pactari {Mona] 399.00 > 99.00 Cene. A 1.000000, Disabled 1.000000 2.000 Rotantioa Tino (nina) t Relativa Rat antion Tina* ' Tina Window (nina) t Paak Selection* 4.140, Enabled 0.000, Oiaablad 0.595 Maaraat | Response Typo* Response Xxtarnal Areas 1 Polynomial Type* j Point of Origin* ) Pit Weighting* J Axis Trena fonati* t U nita* Linear Exclude 1/X Nona Peak-to-poak amplitude* tooothing* rJ Window size (acane)* Nunbar of aaKwtha* 1 Snoothing aathod* 11 Disabled 3 2 i Join valley thraohold* 1 Reduce peak tailing* Raise baseline a u d n a u Draw vertical threshold* Detect shoulders* ! Shoulder threshold* ! 1 Relative height* Absolute height* ` Relative area* ] Absolute area* 20.00 100.00 0.00 ' 10.00 Disebled 40.00 1.50, Disabled 0.00, Disabled 2.00, Disabled 60.00, enabled JI 3 j ! i !.* j j BACK TO MAIN . . l A C o s J x i h l ________ Mathod Aeport Itethod filai u t and! fledi Ce^ouadi Priatadi ' Ci \KASSLYNX\FACT-1 .PR0\MothDS\0521**b.adb Hon lUy 24 07iS9i40 1 2t ZS Man K*y 24 Olitili Iti* Co^euad Nu m i Internai M f . t Quantify Tracci Cane, et Standardi Usar XP Valuat Usar Peak Faeton Integrata M l o d w Faeton ZS [Nona) TIC Fixcd 1.000000, Diaablad 1.000000 2.000 Hatantino Time (mina )i Relativa Ratantico Tiaar Tina Niadow (aina) i PaaJc Salactient 5.120, Aiablad 0.000, Diaablad 0.420 Raaponaa Typai Ramponaci eternai Araaa Polynaaial Typai Poiat of Origini Fit Heightingi A*i Tranafoatieni Unitai Paak-to-paak aaplitudei Linear bcluda X/Z Mona H4 flnoothingi Hiadoa alza (aeansji Munbar of a-- oothai Sttoothing wathodi Diaablad 3 2 Join volley thraaboldi Raduea paak tailingt Raiaa baaaliaa atxiaai 20.00 100.00 0.00 * Draa vartical tbraaholdt Dataci shouldarat Shouldar thraaboldi 10.00 Diaablad 40.00 Relativa heightt Abaoluta hoighti Relativa arcai Abaoluta arcai 1.50, Diaablad 0.00, Diaablad 2.00, Diaablad 0.00. Kiablad . 5oLy? J t e Q L I I C O s /m / H BACK TO MAIN ffl* 2 fluatify Calibratimi Mapatt TOX120-O9 dilutieoa calibratimi! Ci\iaJLYjce\riCT-l.Mo\cur*ba\0S2i m Laat modidadi Mia May 24 MiMidl IBM Priacadi Naa May 24 OPtiaiSB UBB BACK TO MAIN a 1 Bau 0201111 guaatitatad by ami primaad by kjb 09/24/Bf Quutlfy O O U n t i M Mport TOOU20-OJ dilvtlotta ' Calibratimi Ct\nsiUr>ac\ncr-l.Mo\Curraos\0S31ttt lAt aodifidi Mas Itay 34 0Si04t4S ll Printadi M m Hqr 34 MtXOiSl 1 M , * BACK TO MAIN * 3 * . Soup 0301 i Quutltatod by aad priotad ty kb 03/24/J BACK TO MAIN AM ELIA062498: FACT E quipm ent Log 99016 Quattro II,#6448E PAGE DATE: 05/25/99 ANALYST: hoi,sah STUDY NUMBER Tox-120,Tox-026 MATRIX: ratliver,monkey sera DESCRIPTION: dils. FACT Method: cts-M-S.O_____ Injectionvolumc,10uL Cycletime: 9min. Electrode:Z-Spray_____ MobUe Phase: (A)2.0mM NH4CH3CQ2 (B)McOH__________ . TN-A- orstndrdnumber (A) 99012-10-7______ (B)TN-A-2540 Prepared(date/by): Expires: (A)5/21/99ias (A)11/99 .(B)5/18/99 dtb (B) 11/99 Storeatroomtemp? Q or N Other Guard column: None Partnumber na Lot number:na Analyticalcolumn:keystonebetasil_________ ___________ .________ Size:2x 50mm____________ Type:c!8_________ Spheresize:5um Partnumber 055-701-2 Lotnumber P7J20 Serialnumber 038126D Analyticalparameters: Dryinggas(L/hr):300 ESInebulizinggas(L/hr)15 LC Flowrate:300uL/min. Backpressure:70bar Sheathgas(L/hr)0 HPLC solventgradient:9min-2 Time (min.) 1) 0.00 2) 1.0 3 4.5 5) 6.5 LZ____ _ %B 40 40 95 95 40 & - S y f e r h - i ____________ __________________________ Tuning Method Report Method: C :\MASSLYNX\RATS2.PRO\ACQUDB\PFOS Printed: Tue May 25 15:22:53 1999 BACK TO MAIN ' Page 1 MS SOURCE ( ESP- ) Capillary Cone Extractor . RF Lens Source Block Temp. Desolvation Temp. Set Rdbk 3.00 15 5 0.2 150 250 2.98 -15 -5 150 251 Pressures Analyser Vacuum , Gas Cell Rdbk 1.6e-4 4.5e-3 MSI LM Resolution HM Resolution Ion Energy . Ion Energy.Ramp Lens 6 Multiplier Set Rdbk 15.0 15.0 2.0 * o.o 57 650 MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 . Multiplier Set Rdbk 15.0 15.0 30 2.0 0.0 40 0 650 46 40 48 t U ____________________________________ - M asslynx Sam ple L is t Sample List: C:\M ASSLYNX\rats2.PRO\Sam pleDB\052599a.SPL Printed: T ue May 25 15:16:14 1999 .. _ BACK TO MAIN _____________ __________________ Page 1 F ile Name 1 052599001 2 052599002 3 052599003 4 052599004 5 052599005 6 052599006 7 052599007 8 052599008 9 052599009 1 0 052599010 1 1 052599011 1 2 052599012 13 052599013 14 052599014 15 052599015 16 052599016 17 052599017 18 052599018 19 052599019 2 0 052599020 2 1 052599021 2 2 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599027 28 052599028 29 052599029 30 052599030 31 052599031 32 052599032 33 052599033 34 052599034 35 052599035 36 052599036 37 052599037 38 052599038 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043 44 052599044 45 052599045 46 052599046 ` 47 052599047 48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053 54 052599054 55 052599055 56 052599056 57 052599057 58 052599058 59 052599059 60 052599060 File Text methanol blank methanol blank tox120, rbl04199-wblk-1 tox120, rbl04199-lblk-1 tox120, rbl04199-5ppb-1 tox120, rbl04199-10ppb-1 tox120, rbl04199-25ppb-1 tox120, rbl04199-50ppb-1 tox120, rbl04199-100ppb-1 tox120, rbl04199-250ppb-1 tox120, rbl04199-500ppb-1 tox120, rbl04199-750ppb-1 tox120, rbl04199-1000ppb-1 methanol blank tox120, rtl, 3M -g1-250 ppb-ms, 4/19/99 tox120, rtl, 3M -g1-250 ppb-msd, 4/19/99 tox120, rtl, 110F-g1-250 ppb-msd. 2/22/99 tox120, rtl, 110F-g1-250 ppb-msd, 2/22/99 tox120, rtl, 106F-g1-250 ppb-ms, 2/22/99 tox120, rtl, 106F-g1-250 ppb-msd, 2/22/99 tox120, rbl04199-250ppb-cc-4 methanol blank tox120, rtl, 8 1M -g 6 ,4/19/99, d1000 tox120, rtl, 8 2 M -g 6 ,4/19/99, d1000 tox120, rtl, 8 3M -g 6 ,4/19/99, d1000 tox120, rtl, 8 4 M -g 6 ,4/19/99, d1000 tox120, rtl, 8 5M -g 6 ,4/19/99, d1000 tox120, rbl04199-250ppb-cc-1 ' methanol blank tox120, rtl, 8 6M -g 6 ,4/19/99, d1000 tox120, rtl, 8 7 M -g 6 ,4/19/99, d1000 tox120. rU, 8 8M -g 6 ,4/19/99, d1000 tox120, rtl, 8 9M -g 6 ,4/19/99, d1000 tox120, rtl, 9 0M -g 6 ,4/19/99, d1000 tox120, rbl04199-250ppb-cc-6 methanol blank tox120, rtl, 9 1M -g 6 ,4/19/99, d1000 tox120, rtl, 9 3M -g 6 ,4/19/99, d1000 tox120, rtl, 9 4 M -g 6 ,4/19/99, d1000 tox120, rtl, 9 5M -g 6 ,4/19/99, d1000 tox120, rbl04199-250ppb-cc-3 methanol blank tox120, rtl, 17M -g 2,4/19/99, d25 tox120, rtl, 18M -g 2,4/19/99, d25 tox120, rtl, 1 9M -g 2,4/19/99, d25 tox120, rtl, 2 0 M -g 2 ,4/19/99, d25 tox120, rtl, 2 1 M -g 2 ,4/19/99, d25 tox120, rtl, 2 2M -g 2,4/19/99, d25 tox120, rbl04199-250ppb-cc-11 methanol blank tox120, rtl, 1 7M -g 2,4/19/99 tox120, rtl, 18M -g 2,4/19/99 tox120, rtl, 19M -g 2,4/19/99 tox120, rtl, 2 0 M -g 2 ,4/19/99 tox120, rtl, 2 1M -g 2 ,4/19/99 tox120, rtl, 2 2M -g 2 ,4/19/99 tox120, rbl04199-250ppb-cc-2 methanol blank tox120, rtl, 4 1 M -g 3 ,4/19/99 tox120, rbl04199-wblk-1 MS File Inlet File 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN -PFO S-PO A A-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN -PFO S-PO A A-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M1N-PFOS-POAA-513-613 9min-2 . 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M1N-PFOS-POAA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 . 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 ' 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFOS-POAA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9mIn-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFOS-POAA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9mfn-2 9M IN -PFO S-PO A A-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN -P F O S -P O M -513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9m in-2 9M IN-PFOS-POAA-513-613 9min-2 9M IN-PFOS-POAA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9mln-2 ` 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFOS-POAA-513-613 9min-2 9M IN-PFOS-POAA-513-613 9min-2 9M IN-PFOS-POAA-513-613 9mIn-2 9M IN -PFO S-PO A A-5 3-613 9mln-2 9M IN-PFOS-POAA-513-613 9min-2 9M IN-PFOS-POAA-513-613 9M IN-PFOS-POAA-513-613 9M IN-PFOS-POAA-513-613 9M IN-PFOS-POAA-513-613 9m in-2 9m in-2 9m in-2 9m in-2 9M IN-PFOS-POAA-513-613 9mln-2 9M IN-PFOS-POAA-513-613 9mln-2 9M IN-PFOS-POAA-513-613 9min-2 9M IN-PFOS-POAA-513-613 9mln-2 9M IN-PFOS-POAA-513-613 9min-2 BACK TO MAIN b K Sfzs fai__________ ________________ ___________ :--- =-----:----- -------------------------- Masslynx -Sample List ' . Page 2 Sample ListC:\MASSLYNX\rats2.PRO\SampleDB\052599a.SPL Printed:Tue May 2515:16:141999 - File Name 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599069 70 052599070 FileText lox120,rb!04199-lblk-1 tox120,rbl04199-5ppb-1 tox120, rbl04199-10ppb-1 tox120, rbl04199-25ppb-1 taxi20, ibl04199-50ppb-1 tox120, rbl04199-100ppb-1 tox120, rbl04199-250ppb-1 10x120, rbl04199-500ppb-1 tox120, rbl04199-750ppb-1 tox120, rb!04199-1000ppb-1 MS File 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 InletFile 9mirv2 9min-2 9mn-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 f e k l S ( z s ( < t e ______________________________________ canning Method Hapert Method: C:\MRSSUrMC\MIS2.PSO\RCODDB\3MIM-PIOS-PQM-S13-S13 Laat Modified: Tue May 23 10:33:43 1333 ' Printed: Tue May 23 13:13:2 1333 - - loivont Delay { mini ) : 0.00 function : 1 m m of 1 Maaa Pair ( UP - ) Intar Channel Delay { Seca ) t 0.03 Span- ( Daltona ) : 0.00 Start Tine ( Mina ) : 0.00 End Tine ( Mina ) : 9.00 Repaata : 1 Channel Parent Daughter Dwell Sees) Coll Energy (eV) Cone ( V ) 1 413.00 169.00 0.30 30 15 function : 2 MRM of 1 Maas Pair ( ESP- 1 Inter Channel Delay ( Secs ) : 0.03 Span ( Daltona ) : 0.00 Start Tina ( Mina 4 : 0.00 . End Tine ('Mina ) : 9.00 Repeats : 1 Channel Parent Daughter Dwell (Secs) Coll Energy (.V) Cone C V ) 1 313.00 139.00 0.20 30 15 function : 3 MRM of 1 Maaa Pair ( ESP- ) Inter Channel Delay ( Secs ) : 0.03 Span { Daltona ) t 0.00 Start Tine ( Hina ) * 0.00 End Tine ( Hina ) 3 9.00 Repeata : 1 Channel Parent Daughter Dwell (Sacs) Coll Energy (eV) Cone ( V ) 1 613.00 133.00 0.20 30 15 function : 4 MRM of 1 Maaa Pair ( UP- ) Inter Channel Delay { Seca ) t 0.03 Span ( Daltona ) : 0.00 Start Tine ( Mina ) z 0.00 End Tine ( Hina ) : 9.00 Repeat! z 1 Channel Parent Daughter Dwell (Seca) Coll Energy (eV) Cone (*V ) 1 499.00 99.00 0.3Q 45 60 function : 3 MRM of 1 Maaa Pair ( UP- ) Inter Channel Delay ( Seca ) : 0.03 Span ( Deltona ) : 0.00 Start Tina ( Mina ) : 0.00 End Tlan ( Mina I : 3.00 Repeata : >1 Channel Parent Daughter Dwell (Seca) Coll Energy (eV) Cone ( V ) 1 427.00 0.00 0.30 40 30 BACK TO MAIN '_____________________________ ' -- . > Paga i -. . . FACT: DATA REVIEW SUMMARY FORM BACK TO MAIN Date: Analyst: FACT Study Number M atrix Time Points Dilutions? ,- <*5 Analvte 1: TW i f P t m M - S 7 S - LOQ for dus data s e t S ~ lS /y Re-evaluated fcr different range? 4*/' * Analyte 4: Acceptable Linear Range: LOQ for this data set: -O V /S '/ Re-evaluated for different range? . Analyte 2: Acceptable Linear Range: -S ~~ LOQ for Ulis data s e t ^9-- Re-evaluated for different range? Analyte 3: Sh df? Acceptable Linear Range: S~* f & ~~ LOQ for this data s e t Re-evaluated for different range? BACK TO MAIN hb-{ __________ ______________________________________ Quantify Ceopeund luaaary tepori toxl20, rtl _ Saapla Li.tl C:\MMSUNC\rata2.mo\Caaplara\0S2S99a Laat oodlfiad: Had May 2 07:17:2 199 Mathod: C:\MMKn0C\rats2.FIIO\MathDB\052599a Laat aadlflad: ad May 2C 07:19:17 199 Job Coda: Prlntad: Wad May 26 09:20:32 199 ' _________________ ;___________________ -,. Paga 1 .' Compound 1: POH f Maaa 1 052599001 2 052599002 3 052599003 4 052599004 5 052599005 6 052599006 7 052599007 8 052599008 9 052599009 10 052599010 11 052599011 12 052599012 13 052599013 14 052599014 15 052599015 16 052599016 17 052599017 18 052599018 19 052599019 20 052599020 21 052599021 22 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599027 28 052599028 29 052599029 30 052599030 31 052599031 32 052599032 33 052S99033 34 052599034 35 052599035 36 052599036 37 052599037 38 052599038 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043 44 052599044 45 052599045 46 052S99046 47 052599047 48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053 '54 052599054 55 052S99055 56 052599056 57 052599057. 58 052599058 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599069 70 052599070 Sa^laliat Tut *ypa aathanol blank Analyta Rathanoi blank Analyta toxl20, rbl04199-wblk-l Analyta toxi20 rbl04199-lblk-l Analyta toxl20, rbl04199-5ppb-l Standard toxl20, rbl04199-10ppb-l Standard toxl20, rb!04199-25ppb-l Standard toxl20, rbl04199-50ppb-l Standard toxl20, rbl04199-100ppb-l Standard toxl20, rbl04199-250ppb-l Standard toxl20, rbl04199-500ppb-l Standard toxi20, rbl04199-750ppb-l Standard tox!20, rb!04199-1000ppb-l Standard Rathanoi blank 8 H Analyta toxl20, rtl, 3M-01-25O ppb-as, 4/19/99 . ^ Analyta toxi20, rtl, 3M-gl-250 ppb-mad, 4/19/99 toxl20, rtl, 110r-al-2S0 ppb-oajf, 2/22/9 sl*\* Analyta Analyta tox!20, rtl, UOr-gl-250 ppb-mad, 2/22/99 - Analyta texl20, rtl, 10CF-gl-2S0 ppb-na, 2/22/99 Analyta texl20, rtl, lOCF-gl-250 ppb-ud, 2/22/99 Analyta toxl20, rb!04199-2S0ppb-ce-4 - QC aatbanol blank Analyta toxl20, rtl, tlM-gC, 4/19/99, dlOOO Analyta toxl20, rtl, 92H-g6, 4/19/99, dlOOO Analyta toxl20, rtl, 93M-gC, 4/19/99, dlOOO Analyta toxl20, rtl, 94M-g6, 4/19/99, dlOOO Analyta toxl20, rtl, ISM-gC, 4/19/99, dlOOO Analyta toxl20, rbl0419-2S0ppb-ce-l QC aathaxwl blank Analyta toxl20, rtl, lOf-gC, 4/19/99, dlOOO Analyta toxl20, rtl, 7M-gC, 4/19/99, dlOOO Analyta * toxl20, rtl, 98H-gC, 4/19/99, dlOOO Analyta toxl20, rtl, 99M-g6, 4/19/99, dlOOO Analyta toxl20, rtl, W-gC, 4/19/99, dlOOO Analyta toxl20, rbl04199-250ppb-cc-6 QC aatbanol blank . Analyta toxl20, rtl, 91K-g, 4/19/99, dlOOO Analyta toxl20, rtl, 3M-g, 4/19/99, dlOOO Analyta toxl20, rtl, 94M-g6, 4/19/9, dlOOO Analyta toxl20, rtl, SM-gC, 4/19/99, dlOOO Analyta toxl20, rbl04199-250ppb-cc-3 QC Methanol blank Analyta toxl20, rtlv 17M-g2, 4/19/99, d2S Analyta toxl20, rtl, 18M-g2, 4/19/99, d25 Analyta toxl20, rtl, 19K-g2, 4/19/99, d25 Analyta toxl20, rtl, 20M-g2, 4/19/99, d25 Analyta toxl20, rtl, 21M-g2, 4/19/99, d25 Analyta toxl20, rtl, 22M-g2, 4/19/99, d25 Analyta toxl20, rb!04199-250ppb-cc-ll QC aatbanol blank Analyta toxl20, rtl, 17H-g2, 4/19/99 Analyta toxl20, rtl, 19K-g2, 4/19/99 Analyta toxl20, rtl, 19M-g2, 4/19/99 Analyta toxl20, rtl, 20M-g2, 4/19/99 Analyta toxl20, rtl, 21M-g2, 4/19/99 Analyta toxl20, rtl. 22H-g2. 4/19/99 Analyta toxl20, rbl04199-290ppb-cc-2 QC aathanol blank Analyta t0x120, rtl, 41M-g3, 4/19/99 Analyta toxl20, rbl04199-wblk-l Analyta toxl20, rbl04199-lblk-l . Analyta toxl20, rbl04199-Sppb-l Standard toxl20, rbl04199-10ppb-l Standard toxl20, rbl04199-2Sppb-l Standard toxl20, rbl04199-50ppb-l Standard toxl20, rbl04199-100ppb-l Standard toxl20, rbl04199-250ppb-l Standard t0X120, rbl0419-500ppb-l Standard toxl20, rbl04199-750ppb-l Standard toxl20, rbl04199-1000ppb-l ' Standard Std Cono 5.56 11.10 27. B0 55.60 111.00 278.00 556.00 833.00 1111.00 . 278.00 . 278.00 278.00 278.00 278.00 278.00 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 RT 4.950 5.052 5.300 4.985 4.979 4.901 4.924 5.023 5.011 5.028 5.022 4.996 5.033 4.997 5.005 5.000 5.004 5.028 Araa Cono. Flag. Dar 23 121.15 MM 14 70.17 bb 4 8.47 hb 4 6.69 bb 8 30.61 bb 11 48.67 MM 16 82.50 bb 55 314.43 bb 102 599.82 bb 133 786.88 bb 150 889.48 MMX 52 -40 10 -12 -26 13 8 -6 -20 40 228.81 MM 44 252.28 MM 40 223.92 bbx 31 39 173.51 219.73 MM HM l 7 e>iW< 47 268.17 MM / 45 253.71 MM -9 , 5.004 45 256.64 bb -8 5.236 5.042 4 9.54 bb 47 266.91 bb 's -4 5.055 5.042 5.060 5.046 5.023 5.024 5.025 5.026 5.026 5.030 5.024 5.028 5.019 S.024 4.994 5.024 5.030 s 5.044 4.786 5.045 5.027 5.020 5.024 4.972 55 314.02 bb 13 36 201.24 bb ^ 26 139.32 bb , 58 336.91 bb i 55 315.14 bb 1 35 196.68 MM ) 50 283.47 bb j 9 44 247.94 MM -ll 438 2615.70 bb 570 3410.25 bb 523 3130.06 bb 576 3446.03 bb 539 3224.10 bb 442 2640.17 bb 39 217.34 bb 't -22 317 1888.62 MM -K)m A 16 79.50 bb 9 36.60 MMX 20 103.61 bbx 19 97.55 bbX 46 264.89 MMX 79 462.30 MMX 85 497.64 bbx 115 676.08 MMX 32 87 -12 -5 -17 -40 -39 IAC Uc l -V0 sbd Analia 062498: quantlatad and prlntad by hoj 5/26/99 BACK TO MAIN (& J J / S i f o _______________ -- ____________ J ____ :_____:____________________________ ^ Quantify Compound Suanary Kapert toxi20, rtl - Paga a * Sample Lit: C:\MABSLYNX\rafcs2.FRO\8asplaDB\OS2599a Last modified: Wad May 2C 07:17:29 1999 Method: C:\MA3SL3fNX\rats2.PR0\MethDB\052599 Last Modiflad: Wad May 26 07:18:17 1999 Job Codt * .. Printed: Wad May 26 08:20:32 1999 Compound 2: MS 13 f Wane 1 052S99001 2 052599002 3 052599003 4 052599004 S 052599005 6 052599006 7 052599007 052599008 9 052599009 10 052599010 11 052599011 12 052599012 13 052599013 14 052599014 IS 052599015 16 052599016 17 052599017 IS 052599018 19 052599019 20 052599020 21 052599021 22 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599027 28 052599028 29 052599029 30 052599030 31 052599031 32 052599032 33 0S2599033 34 052599034 35 052599035 36 052599036 37 052599037 38 05259903B 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043 44 052599044 45 052599045 46 05259946 47 052599047 48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053 54 052599054 55 052599055 56 0S2599056 57 052599057. 58 052599058 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 0525990(9 70 052599070 Saaplaliat Text methanol blank methanol blank tox!20, rbl04199-wblk-l tox!20, rbl04199-lblk-l toxl20, rblO4199-5ppb**1 toxl20, rb!04199-10ppb-l toxl20, rbl04199-25ppb-l toxl20, rbl04199-50ppb-l toxl20, rbl04199-100ppb-l toxl2Q, rbl04199-250ppb-l toxl20, rbl04199-500ppb-l toxl20, rbl04199-750ppb-l toxl20, rbl04199-1000ppb-l methanol blank toxl20, rtl, 3M-gl-250 ppb-ma,r 4/19/99 Vo* tox!20, rtl, 3H-gl-2S0 ppb-mad, .4/19/99 oil*''4 toxl20, rtl, 110r-gl-250 ppb-as/f, 2/22/99 toxl20, rtl, 110r-gl-250 ppb-ud, 2/22/99 texl20, rtl, lOCF-gl-250 ppb-aa, 2/22/99 toxl20, rtl, lOSF-al-250 ppb-asd, 2/22/99 toxl20, rbl04199-250ppb-cc-4 methanol blank toxl20, rtl, 81M-g6, 4/19/99, dlOOO toxl20, rtl, 82M-g6, 4/19/99, dlOOO toxl20, rtl, 83M-g6, 4/19/99, dlOOO toxl20, rtl, 84M-g6, 4/19/99, dlOOO tox!20, rtl, 85M-g6, 4/19/99, dlOOO toxl20, rb!04199-250ppb-cc-l methanol blank toxl20, rtl, 86M-g6, 4/19/99, dlOOO toxl20, rtl, 87M-g6, 4/19/99, dlOOO ' toxl20, rtl, 88M-g6, 4/19/99, dlOOO toxl20, rtl, 89M-g6, 4/19/99, dlOOO toxl20, rtl, 90M-g6, 4/19/99, dlOOO toxl20, rbl04199-250ppb-cc-6 methanol blank toxl20, rtl, 91M-g6, 4/19/99, dlOOO toxl20, rtl, 93M-g6, 4/19/99, dlOOO toxl20, rtl, 94M-g6, 4/19/99, dlOOO toxl20, rtl, 95M-g6, 4/19/99, dlOOO toxl20, rbl04199-250ppb-cc-3 methanol blank tOX120, rtl, 17M-g2, 4/19/99, d2S toxl20, rtl, 18M-g2, 4/19/99, d25 tOX120, rtl, 19M-g2, 4/19/99, d25 toxl20, rtl, 20M-g2, 4/19/99, d25 toxl20, rtl, 21M-g2, 4/19/99, d2S tOX120, rtl, 22H-g2, 4/19/99, d25 toxl20, rbl04199-250ppb-cc-ll methanol blank tOX120, rtl, 17M-g2, 4/19/99 toxl20, rtl, 18M-g2, 4/19/99 toxl20, rtl, 19M-g2, 4/19/99 toxl20, rtl, 20M-g2, 4/19/99 toxl20, rtl, 21M-g2, 4/19/99 toxl20 rtl 22M-g2, 4/19/99 tox!20, rbl04199-250ppb-cc-2 methanol blank toxl20, rtl, 41M-g3, 4/19/99 t0x120, rb!04199-vblk-l toxl20, rbl04199-lblk-l toxl20, rbl04199-5ppb-l toxl20, rbl04199-10ppb-l toxl20, rbl04199-25ppb-l toxl20, rbl04199-50ppb-l toxl20, rbl04199-100ppb-l toxl20, rbl04l99-250ppb-l toxl20, rbl04199-500ppb-l toxl20, rbl04199-750ppb-l tox!20, rb!04199-1000ppb-l t Analyte Analyte Analyte Analyte Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyte Analyte Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta OC Analyta Analyta Analyte Analyte Analyta Analyta OC Analyta Analyta Analyta Analyte Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyte Analyte Analyta Analyta QC Analyta Analyte Analyte Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard 8td Cono 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 . 278.00 278.00 278.00 278.00 278.00 278.00 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 ta 5.471 5.488 5.464 5.488 S.455 5.469 5.465 5.750 5.462 5.475 5.463 5.476 5.468 5.494 5.446 5.470 5.470 ' 5.486 5.476 5.460 5.502 5.513 5.489 5.513 5.494 5.515 5.492 5.471 5.490 5.471 S.486 5 .4 8 8 5.485 5.468 5.465 * 5.510 5.501 5.488 5.470 5.464 5.465 5.487 Ara Cone. Flags %Dev 5 12.91 bb -54 13 72.45 MM 30 21 130.40 bb 17 46 316.68 MM 14 77 540.85 bb -3 110 788.13 MM -5 129 922.50 MMX -17 4 0.98 bb 34 226.51 bb*2 39 262.87 bbj 28 180.25 bb"j 26 162.57 bb \ 41 277.30 MM .) 42 283.04 bb> 36 240.93 MM -13 - 5 11.89 bb 37 245.31 MM -12 lAc p s M v 40 267.80 bb -4 34 228.46 bb -18 9 40.68 bb \ 6 16.57 MM ) _ I l 13 67.85 bb y K W d 16 88.95 bb 21 14 125.53 80.77 bb bb /// 32 208.72 MM -25 155 120 138 . 157 161 104 33 1113.48 KM 861.12 bb 987.88 MM 1131.41 bb 1161.29 bb 741.94 bb 216.29 MM -22 91 643.40 bb lA C 2 0.00 bbX -100 9 37.86 MMX 36 11 52.34 bbX -6 22 139.09 bbX 25 34 223.39 bbX -20 61 427.20 bbX -23 71 496.09 bbX -40 74 520.73 bbX -53 Anali 062498: quantitad and printed by hoj 5/26/99 BACK TO MAIN Quantify Compound t a w y Kapert TQX-120 Kat Liver Dilutions Sample Lit: S:\IACT-l.PRO\aaapleDB\OSM99a Last modified: Mon May 17 14:14:20 1999 Method: 8 :\KACT-1.PKO\MethOB\OS1499a Last modified: Mon Nay 17 14:23:38 1999 Job Code: Printed: Tue May It 08:22:40 1999 Compound 2: M4S3 t Ham* 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 ? 051499067 8 051499068 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 17 051499077 18 051499078 19 051499079 20 051499080 21 051499081 22 051499082 23 051499083 24 051499084 25 051499085 26 051499086 27 051499087 28 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114 Saaqjleliat Test M*OB Blank MaOH Blank . Tox-120, RBL04199-H2OBlk-2, 4/19/99 Tox-120, RBL04199-LBlk-2, 4/19/99 Tox-120, RBL04199-5ppb-2, 4/19/99 Tox-120, RBL04199--10ppb-2, 4/19/99 Tox-120, RBL04199-25ppb-2, 4/19/99 Tox-120, RBL04199-50ppb-2, 4/19/99 Tox-120, HBL04199-100ppb-2, 4/19/99 Tox-120, RBL04199-250ppb-2, 4/19/99 Tox-120, RBL04199-500ppb-2, 4/19/99 Tox-120, KBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-1000ppb-2, 4/19/99 MaOH Blank Tox-120 23M 62-/ 111:25 . Tox-120 24M 62 / Dl:25 - Tox-120 25M 62 / D1:2S Tox-120 2 G2 / Dl:25 Tox-120, RBL04199-250ppb-CCl, 4/19/99 MaOH Blank Tox-120 33M 63 / Dl:25 Tox-120 34M 63 / 01:25 Tox-120 35M 63 / 01:25 TOX-120 36M 63 / 01:25 Tox-120 37M 63 / 01:25 Tox-120 3SM 63 / Dl:25 Tox-120 39M 63 / 01:25 Tox-120 40K 63 / 01:25 Tox-120, KBL04199-250ppb-CC3, 4/19/99 MaOH Blank . Tox-120 27M 62 / 01:10 Tox-120 29M 62 / Dl:10 Tox-120 3 62 / 01:10 Tox-120 31M 62 / 01:10 Tox-120, XBL04199--250ppb--CC4, 4/19/99 MaOH Blank Tox-120 42M 63 / 01:10 Tox-120 43M 63 / Dl:10 Tox-120 45M 63 / 01:10 Tox-120 46M 63 / Dl:10 Tox-120 47M 63 / 01:10 Tox-120, RBL04199-250ppb-CC5, 4/19/99 MaOH Blank Tox-120, RBL04199-H2QBlk-2, 4/19/99 Tox-120, RBL04199-LBlk-2, 4/19/99 Tox-120, RBL04199-Sppb-2, 4/19/99 Tox-120, RBL04199-10ppb-2, 4/19/99 Tox-120, RBL04199-25ppb~2, 4/19/99 Tox-120, RBL04199-50ppb-2, 4/19/99 Tox-120', KBL04199-100ppb-2, 4/19/99 TOX-120, RBL04199-250ppb--2, 4/19/99 Tox-120, KBL04199-S00ppb-2, 4/19/99 Tox-120, HBL04199-750ppb-2, 4/19/99 Tox-120, RBL04139--1000ppb-2, 4/19/99 Type Analyte Analyte Analyte Analyte Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyte Analyte Analyte Analyta Analyte QC Analyta Analyte Analyte Analyte Analyte Analyte Analyte Analyte Analyta QC Analyte Analyta Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard 8td Cono R 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 278.00 5.307 5.306 * 5.307 5.307 5.306 5.308 5.308 5.308 5.315 . 5.307 5.306 5.307 278.00 278.00 5.308 5.306 5.307 5.307 278.00 5.306 5.307 5.306 5.307 5.306 5.56 11.10 27.80 S5.60 111.00 278.00 556.00 833.00 1111.00 Arti 613 637 509 473 48 49 47 44 44 35 44 43 1080 1367 1207 1023 66 83 50 63 55 ItaaponM Cone. Flaya 612.731 636.927 509.037 472.806 bbz bbZ bbx bbZ 48.048" '48.561 46.922 43.598 43.676 34.651 44.313 42.794 MMI MHZ MMX MMX MMX MMX MMX MMX 1079.684 1367.145 1206.705 1022.573 bbl bbl bbl bbl 66.125 83.086 SO.209 63.197 55.155 MMX bbl MMX MMX MMX * Page 2 Soup 020199, Reviewed and quantitated by drb 5/17/99 BACK TO MAIN f J s i \ S l ~ i c C ^ _____________________________________________ ' ____________________________________-- Quantify Conpound Sunury taper ter120, rtl _ ,, Page a tanple Urti C:\MMaunK\rata2.PR0\taapleDB\05259ta Lait modified: ed May 2 07:17:29 1999 Method: C:\MMatXMX\rata2.PRO\MathDB\OS2599a last modified: Wad May 29 07:11:17 1999 Job Coda: ' . . Printed: ad May 2C 0t:20:32 1999 Ceapound 3: MC13 Hama Samplaliat Text Type 8td Cone R Area Cono. nega %Dv 1 0S2599001 methanol blank Analyte 2 052599002 methanol blank Analyte 3 052599003 toxl20, rbl04199~wblk-l Analyte 4 052599004 toxl20, rb!04199-lblk-l Analyte 5 052599005 toxl20, rbl04199-5ppb-l Standard 5.56 5.795 3 1.54 MM -72 6 052599006 toxl20, rbl04199-10ppb-l Standard 11.10 5.797 5 8.66 MM -22 7 052599007 toxl20, rbl04199-25ppb-l Standard 27.00 5.765 14 32.23 bb 16 8 052599008 toxl20, rbl04199-50ppb-l Standard 55.60 5.808 33 82.54 bb 48 9 052599009 toxl20, rbl04199-100ppb-l Standard 111.00 5.784 S3 137.72 bb 24 10 052599010 toxl20, rbl04199-250ppb-l Standard 278.00 5.782 116 304.34 bb 9 11 052599011 toxl20, rbl04199-S00ppb-l Standard 556.00 5.776 221 587.57 bb 6 12 052599012 toxl20, rbl04199-750ppb-l Standard 833.00 5.767 318 848.84 bb 2 13 052599013 toxl20, rbl04199-1000ppb-l Standard 1111.00 5.788 369 985.61 bb -11 14 052599014 methanol blank X J Analyte 3 IS 052599015 16 052599016 17 052599017 toxl20g rtl, 3M-gl-250 ppb-ma/, 4/19/99 ,vt. toxl20, rtl, 3M-gl-2S0 ppb-asd, 4/19/99 toxl20, rtl, llOP-gl-250 ppb-W, 2/22/99 Analyte Analyte Analyte 5.782 5.778 \ - 5.784 105 274.93 bb 118 310.32 MM 104 273.67 MM\ 18 052599018 19 052599019 toxl20, rtl, 110P-gl-2S0 ppb-med, 2/22/99 texl20, rtl, 106C-gl-2SO ppb-ne, 2/22/99 Analyte Analyte 5.772 5.765 92 102 241.88 MM 1 r*.1.__t 268.45 bb l ehtertCft lAt O J Jf a i n 20 052599020 tox!20, rtl, lOCF-gl-250 ppb-med, 2/22/99 Analyta 5.767 120 315.53 MH J 21 052599021 toxl20, rbl04199-250ppb-cc-4 QC 278.00 5.767 101 266.49 MM -4 22 052599022 nethanol blank Analyta 23 052599023 toxl20, rtl, lM-gS, 4/19/99, diooo Analyte 24 052599024 toxl20, rtl, 82M-J6, 4/19/99, dlOOO Analyta 25 052599025 taxl20, rtl, 3M-g6, 4/19/99, dl000 Analyte 26 052599026 texl20, rtl, 84M-gt, 4/19/99, dlOOO Analyte 27 052599027 toxl20, rtl, SK-ge, 4/19/99, dlOOO Analyta 28 052599028 toxl20, rbl04199-2S0ppb-cc-l QC 278.00 5.792 112 295.72 MM 6 29 052599029 nethanol blank Analyta 30 052599030 toxl20, rtl, 86K-gf, 4/19/99, dlOOO Analyta 31 052599031 toxl20, rtl, 7M-g, 4/19/99, dlOOO Analyta 32 052599032 * toxl20, rtl, am-gf, 4/19/99, dlOOO Analyte 33 052599033 toxl20, rtl, 89M-g, 4/19/99, dlOOO Analyte 34 052599034 toxl20, rtl, 90M-gC, 4/19/99, dlOOO , Analyta 35 052599035 tox!20, rbl04i99-250ppb-cc-6 QC 278.00 5.782 112 295.27 Kf 6 36 052599036 nethanol blank Analyta 37 052599037 toxl20, rtl, 91M-gC, 4/19/99, dlOOO Analyte 38 052599038 toxl20, rtl, 93M-gt, 4/19/99, dlOOO Analyta 39 052599039 toxl20, rtl, 94M-g6, 4/19/99, diooo Analyte 40 052599040 texl20, rtl, 5M-g, 4/19/99, diooo Analyta 41 052599041 toxl20, rbl04199-250ppb-cc-3 QC 278.00 5.795 100 262.77 MM -5 42 052599042 nethanol blank Analyta 43 052599043 . toxl20, rtl, 17K-g2, 4/19/99, d25 Analyta 5.781 s 8.69 bb 44 052599044 toxl20, rtl, 18M-g2, 4/19/99, d25 Analyta 45 052599045 toxl20, rtl, 19M-g2, 4/19/99, d25 Analyta 46 052S99046 toxl20, rtl, 20K-g2, 4/19/99, d25 Analyta 47 052599047 toxl20, rtl, 21M-g2, 4/19/99, d25 Analyta 48 052599048 toxl20, rtl, 22M-g2, 4/19/99, d?5 Analyta 49 052599049 toxl20, rbl04199-2S0ppb-ce-ll QC 278.00 5.013 76 199.37 MM -28 50 052599050 methanol blank Analyte 51 052599051 toxl20, rtl, 17M-g2, 4/19/99 J 52 052599052 S3 052599053 54 052599054 5S56 0055225S9999005S56 toxl20, rtl, 16M-g2, 4/19/99 toxl20, rtl, 19M-g2, 4/19/99 toxl20, rtl, 20M-g2, 4/19/99 toxl20, rtl, 21M-g2, 4/19/99 toxl20, rtl, 22M-g2, 4/19/99 Analyta Analyta Analyta Analyta Analyte analyta 5.812 5.791 5.780 5.8X5 5.783 5.785 f36 89.73 MK 28 69.25 bb a } 33 84.01 bb S1 t h k n 30 73.71 bb ( 39 29 98.92 72.06 bb bb >j1 IAC. 0 57 052599057 toxl20, rbl04199-250ppb-cc-2 QC 271.00 5.782 81 210.54 MM -24 58 052599058 nethanol blank Analyta 59 052599059 toxl20, rtl, 41M*g3, 4/19/99 Analyta 5.789 27 66.29 bb 60 052599060 toxl20, rbl04199'Wblk-l Analyta 61 052599061 toxl20, rbl04199-lblk-l Analyte 62 052599062 toxl20, rbl04199-Sppb-l Standard 5.56 5.741 v 9 17.56 bbX 216 63 052599063 toxl20, rbl04199-10ppb-l Standard 11.10 5.807 X 7 11.87 bbX 7 64 052599064 toxl20, rbl04199-2Sppb-l standard 27.80 5.806 . 13 29.34 bbX 6 65 052599065 toxl20, rbl04199-S0ppb-l Standard 55.60 5.775 11 23.51 bbX -58 66 052599066 toxl20, rbl04199~100ppb~l Standard 111.00 5.786 41 103.06 bbX -7 67 052599067 toxl20, rbl04199-2S0ppb-l Standard 278.00 5.790 87 227.94 bbX -18 68 052599068 toxl20, rbl04199-S00ppb-l Standard 556.00 5.705 150 398.05 bbX -28 69 052599069 70 052599070 toxl20, rbl04199-7S0ppb-l tox!20, rb!04199-1000ppb-l Standard Standard 1181313..0000 5.787 5.786 204 542.63 bbX -35 252 669.29 bbX -40 f A M l i a 062498: quentieted and printed by hoj 3/26/99 } i i BACK TO MAIN (4>SS(^k<j ____________ ;__________________________ :____ :______:________________________________ -- Quantify Compound iuua r y Rapprt OSX20 , rtl - . Pag* 4 S u p l o List! C:\MMSUniX\roto2.FllO\SuploDB\OS2599B Loot aodiflod: od Kay 2 07:17:29 1999 Method: C:\MfcSSUmx\rata2.9RO\HathDB\052599a Loot aodiflod: od May 2 07:10:17 1999 Job Cod: - Printed: Wed Hy 26 08:20:32 1999 Coopound 4: FFOS 052599001 052599002 052599003 052599004 052599005 052599006 052599007 052599008 052599009 10 052599010 11 052599011 12 052599012 13 052599013 14 052599014 15 052599015 16 052599016 17 052599017 18 052599018 19 052599019 20 052599020 21 052599021 22 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599027 28 052S99028 29 052599029 30 052599030 31 052599031 32 052599032 33 052599033 34 052599034 35 052599035 36 052599036 37 052599037 38 052599038 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043 44 052599044 45 052599045 46 052599046 47 052599047 48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053 54 052599054 55 052599055 56 052599056 57 052599057. S8 052599058 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599069 70 052599070 Sasplelist Text ethanol blank ethanol blank toxl20, rbl04199-wblk-l toxl20, rbl04199-lblk-l tox!20, rbl04199-5ppb-l toxl20, rbl04199-10ppb-l toxl20, rbl04199-25ppb-l toxl20, rbl04199-50ppb-l tox!20, rbl04199-100ppb-l toxl20, rbl04199-250ppb-l toxl20, rb!04199-500ppb-l toxl20, rbl04199-750ppb-l toxl20, rbl04199-1000ppb-l methanol blank toxl20, rtl, 3M-gl-250 ppb-as, 4/19/99 toxl20, rtl, 3M-gl-250 ppb-asd, 4/19/99 toxl20, rtl, 110F-gl-250 ppb-ns^ 2/22/99 toxl20, rtl, 110F-gl-2S0 ppb-mad, 2/22/99 toxl20, rtl, 106F-gl-250 ppb~ns, 2/22/99 toxl20, rtl, 106F-gl-250 ppb-mad, 2/22/99 toxl20, rbl04199-250ppb-cc-4 ethanol blank tOX120, rtl, 81M-g6, 4/19/99, dlOOO toxl20, rtl, 82M-g6, 4/19/99, dlOOO toxl20, rtl, 83M-g6, 4/19/99, dlOOO toxl20, rtl> 84M-g6, 4/19/99, dlOOO toxl20, rtl. 5M-g6, 4/19/99, dlOOO toxl20, rbl04199-250ppb-cc-l ethanol blank tox!20, rtl, 86H-g6( 4/19/99, dlOOO t0x 120, rtl, 87M-g6( 4/19/99, dlOOO toxl20, rtl, 88K-gS, 4/19/99, dlOOO toxl20, rtl, B9M-g6( 4/19/99, dlOOO tox!20, rtl. 90M-g6, 4/19/99, dlOOO toxl20, rbl04199-250ppb-cc-6 ethanol blank toxl20, rtl, 91M-g6, 4/19/99, dlOOO toxl20, rtl, 93H-g6, 4/19/99, dlOOO toxl20, rtl, 94H-g6, 4/19/99, dlOOO toxl20, rtl, 95M-g6, 4/19/99, dlOOO toxl20, rbl04199-250ppb-cc-3 ethanol blank . t0x120, rtl, 17M-g2, 4/19/99, d25 toxl20, rtl, 18M-g2, 4/19/99, d25 t0x120, rtl, 19M-g2, 4/19/99, d25 tOX120, rtl, 2QK-g2, 4/19/99, d25 t0x120, rtl, 21M-g2, 4/19/99, d25 toxl20, rtl, 22M-g2, 4/19/99, d25 toxl20, rbl04199-250ppb-cc-ll ethanol blank toxl20, rtl, 17H-g2, 4/19/99 toxl20, rtl, 18M-g2, 4/19/99 toxl20, rtl, 19M-g2, 4/19/99 t0x120, rtl, 20M-g2, 4/19/99 toxl20, rtl, 21M-g2, 4/19/99 tOXl20, rtl, 22M-g2, 4/19/99 t0x120, rbl04199-250ppb-cc-2 methanol blank toxl20, rtl, 41M-g3, 4/19/99 toxl20, rbl04199-wblk-l t0X120, rbl04199-lblk-l toxl20, rbl04199-5ppb-l toxl20, rbl04199-1Oppb-l toxl20, rbl04199-2Sppb-l tox!20, rbl04199-50ppb-l toxl20, rbl04199-10Oppb-l t0X120, rbl04199-250ppb-l toxl20, rb!04199-500ppb-l toxl20, rbl04199-7S0ppb-l toxl20, rb!04199-1000ppb-l Typ Std Cono Analyte Analyte Analyte Analyte Standard 5.56 Standard 11.10 Standard 27.80 Standard Standard 55.60 111.00 Standard 278.00 Standard 556.00 Standard Standard 833.00 1111.00 Analyte Analyte Analyte Analyte Analyte Analyte Analyte QC 278.00 Analyte Analyte Analyte Analyte Analyte Analyte QC 278.00 Analyte Analyte Analyte Analyte Analyte Analyte QC . 278.00 Analyte Analyte Analyte Analyte Analyte QC 278.00 RT 5.329 5.283 5.264 5.268 5.259 5.278 5.277 5.255 5.269 5.284 5.278 5.275 5.266 5.278 5.266 5.255 5.261 5.262 5.245 5.288 5.280 5.283 5.282 5.285 5.284 5.285 5.260 5.274 5.295 5.278 5.273 5.232 5.284 5.263 5.279 5.294 5.288 Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Standard Standard Standard Standard Standard Standard Standard Standard Standard 278.00 278.00 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00 5.300 5.294 5.280 5.282 5.282 5.284 5.281 5.287 5.300 5.303 5.275 5.284 5.302 5.283 5.279 5.277 5.262 5.254 5.282 5.279 5.2S6 5.277 ' Cono. Flage %Dv 13 34 42 43 92 129 195 445 861 1119 1327 496 504 627 666 516 506 440 5 361 693 494 600 669 405 679 634 566 466 470 404 9 389 311 370 367 365 6 7 16 0.00 bb 0.00 bb 3.40 MM 4.66 bb 41.91 bb 69.73 bb 120.06 bb 310.85 bb 628.49 bb 825.42 bb 984.54 MM -39 -58 51 25 8 12 13 -1 -11 350.27 bb 356.00 bb 450.18 bb 480.05 bb 365.37 bb 357.63 bb gnUruI 307.74 bb u UtC M w 0.00 bb 246.90 bb 500.27 bb 348.85 bb 429.18 bb 482.06 bb 280.89 bb ' & \ u e A LAC O f 2 1 * 1 `j j 489.72 bb 455.76 bb 403.35 bb 326.97 bb 330.46 bb 279.77 bb 0.00 MM 268.32 bb 209.11 bb 254.08 bb 251.43 bb 250.18 bb -10 0.00 bb 0.00 bb 0.00 bb 279 81 64 66 54 59 52 339 61 21 29 40 57 87 165 325 590 800 983 184.35 MM 33.46 bb 20.62 bb 22.28 bb 12.57 bb 16.68 bb 11.05 MM 229.96 bb -34 ft -17 18.46 bb 0.00 bb 0.00 MMX 1.77 MMX 14.92 bbX 37.99 bbX 97.67 MMX 219.58 bbX 421.63 bbX 582.34 bbX 721.54 bbX -100 -84 -46 -32 -12 -21 -24 -30 -35 Amelia 062498: quantiatad and printed by hoj 5/26/99 [d s A J f y f o y ___________________ Quantify Csapound Svaaaary Kaport toxl20 , rtl Saada U s t : C : \ M M S U m x \ n t a 2 . PRO\Suvlal\0S3S99a Laat sodifiad: Wad May 2f 07:17:2 199 Mathed: C:\MM8L7HX\rata2.PI\Matid\0S2Sa Laat aedlflad: Wad May 2C 07:19:17 1999 Job Coda: Prlntad: Wad May 2 09:20:32 199 Ccnyonnd S: 19 # NRM 1 052599001 2 052599002 3 052599003 4 052599004 5 052599005 4 052599006 7 052599007 8 052599008 9 052599009 10 052599010 11 052599011 12 052599012 13 052599013 14 0S2599Q14 15 052599015 16 052599016 17 052599017 18 052599018 19 052599019 20 052599020 21 052599021 22 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599027 28 052599028 29 052599029 30 052599030 31 052599031 32 052599032 33 052599033 34 052599034 35 052599035 36 052599036 37 052599037 38 052599038 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043 44 052599044 45 052599045 46 052599046 47 052599047 48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053 54 052599054 55 052599055 56 0S2S990S6 57 052599057 58 052599058 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599069 70 052599070 faaplaliet Text ethanol blank typ Analyte ethanol blank Analyte toxl20# rbl04199-wblk-l Analyte taxi20, rbl04199-lblk-l Analyte toxl20, rb!04199-5ppb-l Standard toxl20, rbl04199-1Oppb-1 Standard toxl20, rb!04199-25ppb-l Standard tox!20, rbl04199-50ppb-l Standard. toxl20, rb!04199-100ppb-l Standard toxl20, rbl04199-250ppb-l Standard toxl20, rbl04199-500ppb-l Standard toxl20, rbl04199-750ppb-l Standard tox!20, rbl04199-1000ppb-l ethanol blank (V\SC ) X Standard Analyte tox!20, rtl, 3M-gl-250 ppb-u,, 4/19/99 lk,- Analyte toxl20, rtl, 3H-gl-250 ppb-aad, 4/19/9 Analyte toxl20, rtl, 110F-gl-250 ppb-oad, 2/22/99 ilw.lV Analyte toxl20, rtl, 110r-gl-250 ppb-nad, 2/22/99 Analyte toxl20, rtl, 106r-gl-250 ppb-u, 2/22/99 Analyte toxl20, rtl, 106I-gl-250 ppb-ud, 2/22/99 Analyte toxl20, rbl04199-25Oppb-cc-4 QC ethanol blank Analyte toxl20, rtl, 81M-g6, 4/19/99, dlOOO Analyte toxl20, rtl, 82M-g6, 4/19/99, dlOOO Analyte toxl20, rtl, 03H-g6, 4/19/99, dlOOO Analyte toxl20, rtl, 84H-g6, 4/19/99, dlOOO Analyte toxl20, rtl, 85M-g6, 4/19/99, dlOOO Analyte toxl20, rbl04199-250ppb-cc-l QC methanol blank Analyte toxl20, rtl, 86M-g6, 4/19/99, dlOOO Analyte toxl20, rtl, 87K-g6, 4/19/99, dlOOO Analyte * tox!20, rtl, 88H-g6, 4/19/99, dlOOO Analyte toxl20, rtl, 89M-g6, 4/19/99, dlOOO Analyte toxl20, rtl, 90H-g6, 4/19/99, dlOOO Analyte toxl20, rbl04199-250ppb-cc-6 QC methanol blank Analyte toxl20, rtl, 9lM-g6, 4/19/99, dlOOO Analyte toxl20, rtl, 93M-g6, 4/19/9$, dlOOO Analyte toxl20, rtl, 94K-g6, 4/19/99, dlOOO Analyte toxl20, rtl, 95M-g6, 4/19/99, dlOOO Analyte tOX120, rbl04199-250ppb-ce-3 QC methanol blank Analyte toxl20, rtl, 17M-g2, 4/19/99, d25 Analyte * toxl20, rtl, 18H-g2, 4/19/99, <125 Analyte tOX120, rtl, 19K-g2, 4/19/99, d25 Analyte tOX120, rtl, 20M-g2, 4/19/99, d25 Analyte toxl20, rtl, 21M-g2, 4/19/99, d25 Analyte toxl20, rtl, 22M-g2, 4/19/99, d?5 Analyte toxl20, rbl04199-250ppb-cc-ll QC methanol blank Analyte toxl20, rtl, 17H-g2, 4/19/99 Analyte toxl20, rtl, 18H-g2, 4/19/99 Analyte toxl20, rtl, 19X-g2, 4/19/99 Analyte toxl20, rtl, 20M-g2, 4/19/99 Analyte toxl20, rtl, 21M-g2, 4/19/99 toxl20, rtl, 22H-g2, 4/19/99 Analyte Analyte toxl20, rbl04199-250ppb-cc-2 QC methanol blank Analyte toxl20, rtl, 41M-g3, 4/19/99 Analyte toxl20, rbl04199-wblk-l Analyte toxl20, rb!04199-- lblk-1 Analyte toxl20, rb!04199-5ppb-l Standard toxl20, rbl04199-1Oppb-1 Standard toX120, rbl04199-25ppb-l Standard toxl20, rbl04199-50ppb-l Standard toxl20, rbl04199-100ppb-l Standard toxl20, rbl04199-25Oppb-1 Standard tox!20, rbl04199-500ppb-l Standard toxl20, rbl04199-750ppb-l Standard tox!20, rbl04199-1000ppb-l Standard 8td Cono 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 * 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 KT 5.042 5.022 4.999 5.004 5.022 4.993 4.992 4.993 4.981 4.999 4.993 4.991 5.003 4.992 4.999 5.018 4.999 4.998 5.022 * 5.012 5.025 5.010 5.029 5.041 5.018 5.019 5.046 5.020 5.020 5.024 5.018 4.998 5.013 4.994 5.012 4.995 5.001 5.017 5.022 5.016 4.989 5.000 4.992 4.997 4.991 4.992 4.991 Area 826 ' 1437 1653 1452 1463 1461 1233 1312 1146 1022 1071 1104 1260 197 . 200 219 235 1566 1182 1232 1129 43 47 44 43 29 52 952 488 529 446 570 446 512 1067 518 800 1085 v 1431 * 1233 .1209 1262 1219 1055 1099 983 1054 Cono. Flage %DV 0.66 bb 1.16 bb 1.33 bb 1.17 bb 1.18 bb 1.18 bb 0.99 bb 1.06 bb 0.92 bb 0.82 bb 0.86 bb 0.89 bb 1.01 bb 0.16 bb 0.16 bb . 0.18 bb 0.19 bb 1.26 bb -34 16 33 17 18 18 -1 6 -8 -18 -14 -11 1 -04 -84 -82 -81 26 0.95 bb -5 0.99 bb -1 0.91 bb ' 0.03 bb 0.04 bb 0.04 bb 0.03 bb 0.02 bb 0.04 bb 0.77 bb 0.39 bb 0.43 bb 0.36 bb 0.46 bb 0.36 bb 0.41 bb 0.86 bb 0.42 bb 0.64 bb 0.87 bb 1.15 bb 0.99 bb 0.97 bb 1.02 bb 0.98 bb 0.85 bb 0.88 bb 0.79 bb 0.85 bb -9 -97 -96 -96 -97 -98 -96 -23 r61 -57 -64 -54 -64 -59 .-14 -58 -36 -13 15 -1 -3 2 -2 -15 -12 -21 -15 9mgm S Aawlia 0C2499: quantlatad and printad by koj 5/26/99 Quantify Calibratio n Itaport toxl20 , rtl Calibrati! e : \ M M 8 U K X \ r a t a 2 . m o \ C u mM \ 0 S 2 S 9 9 a Laat andifiad: ad May 26 07:56:39 1999 Printad: ad May 26 06:21:03 1999 BACK TO MAIN *> X Quanttfy Calibration Kaport taxi20, r U C*libration i C:\MU9UrMX\rata2.9IIO\CuniaBB\052S99a Laat aodifiad: Wad May 2 07:36:39 199 Frintad: ad May 2S 09:21:03 1999 - - BACK TO MAIN . *Ba 2 ' * Quantify Calibration Xaport toxl20 , rtl ' Calibration: C:\MM9UnoC\rata2.mo\CurvaOB\OS2599a Laat nodifiad: Wad Kay 2 07:56:39 1999 Printad: Wad May 26 09:21:05 1999 Compound 3 name: M613 : ^ Coefficient o f Determination: 0.983551 Calibration curve: 0.372617 * x + 2.17690 Response type: External Std, Area Curve type: Linear, Origin: Exclude, Weighting: 1/x, Axis trans: None ] J 1 TI 5}i I I iI i 1i A a a U a 0624981 quantlatad and printad b y hoj 5/26/99 BACK TO MAIN Quantify Calibration pert toa!20 , rtl . Calibration! C:\MMlUMX\rata2.PIIO\CurvuD8\032599a taafc aodifiad: W ad May 2 07:54:39 1999 Prlntad: Had May 2 09:21:03 1999 BACK TO MAIN Aaalla 062498: quaatiatad and prlntad b y hoj S/24/99 Quantity Calibration Raport toxl20, xtl Calibration C:\MAS8UNX\xata2.PII0\ClirvaD5\0S2599a Laat aodlflad: ad May 28 07:58:39 1999 Printad: od May 28 08:21:05 1999 ' ' - . BACK TO MAIN Paga 8 . Amalia 082498: q u an tiatad and p rin ta d by be] 5/28/99 Method Report Method filit Last modified: Compound: C:\MASSLnoC\rats2.PR0\MethDB\O52599a.mdb Nad May 2 07:18:17 1999(3 1: p o m Printed: Had May 28 08:21:19 1999 Compound Nano: Internal Ref.: Quantify Trace: Cone, of Stendardo: Veer RF Value: dear Peak Factor: Integrate Window Factor: Peak location POM [Mona] TIC Cone. A 1.000000, Dioabled 1.000000 2 .0 0 0 Alhtlia Retention Time (nina): Relativa Retention T i m : T i m Window (nine) : Peak Selection: Response S.010, Enabled 0.000, Disabled 0.304 Nearest Response Type: Response: Calibration Curves . Polynomial Type: Point of Origin: rit Weighting: Axis Transformation: Concentration External Areas Linear Exclude 1/X None Units: Noise Peak-to-peak amplitude: Smooth so Smoothing: Window size (scans): Number of smooths: Smoothing method: Baselines 4 Disabled 3 2 Join valley threshold: Reduce peak tailing: ' Raise baseline maximum: Peak Separation 30.00 70.00 4.00 Draw vertical threshold: Detect shoulders: Shoulder threshold: 1 Response Threshold ' Relative height: Absolute height: Relative area: Absolute area; 1 0 .0 0 Disabled 10.00 50.00, Enabled 0.00, Disabled 3.00, Enabled 0.00, Disabled A.c o s / jc./ m BACK TO MAIN Faga 1 Method Report Hathod flit! Last modiiied: C:\KftSSLyNX\rats2.PR0\HathDB\O52599a.mdb Had Hay 26 07:19:1? 19990 ' - Compound: Printed: 2: M513 Had Hay 26 09:21:19 1999 /) / __________________________________________ A l t o t M / i DLUHWni'iilOV____ Ut or/adu Compound Hama: Internal Ref.: Quantity Trace: Cone, of Standard: MS 13 [None] TIC Cone. A Osar RF Value: Osar Peak Factor: Integrate Hindov Factor: 1.000000, Disabled 1.000000 2.000 Peak Location Retention Time (mins): Relativa Retention Time: Time Hindow (mins): 5.551, Enabled 0 .000, Disabled 0.233 Peak Selection: Hearest Response * Response Type: Response: External Areas Calibration Curves Polynomial Type: Point of Origin: rit Weighting: Axis Transformation: Linear Exclude l/X Hone Concentration Units: Noise Paak-to-peak amplitude: 64 Smooth Smoothing: Window size (scans): Number of smooths: Smoothing method: * Disabled 3 2 Baselines Join valley threshold: Reduce peak tailing: * Raisa baseline maximum: 30.00 70.00 4.00 Peak Separation Draw vertical threshold: Detect shoulders: Shoulder threshold! 10.00 Disabled 10.00 Response Threshold * Relative height: absolute height: Relative area: Absolute area; 50.00, Enabled 0.00, Disabled 3.00, Enabled ' 0.00, Disabled * BACK TO MAIN BACK TO MAIN Method Report Method file: teat aodlfled: C:\KA3SLyNX\rets2.PRO\MethDB\052599e.adb Ned May 2 07:18:17 10990 Compound: 3: Mil3 Printed: Med May 28 08:21:19 1999 Compound Nanai Internal Ref.i Quantify Trace: Cone, of Standardl M613 (Nona) TIC Cone. A Ar*ie^.QL2*- User RF Value: User Peak Factor: Integrate Window factor: 1.000000, Diaabled 1.000000 2.000 Peak Location Retention Tine (mins): Relative Retention Time: Time Window- (mins) : S.861, Enabled 0.000, Disabled 0.219 Peak Selection: Nearest Response Response Type: Response: External Areas Calibration Curves Polynonial Type: Point of Origin: Fit Weighting: Axis Transformation: Concentration linear Exclude 1/X None Units: Noise . Peak-to-peak amplitude: 79 Smooth Smoothing: Window size (scans): Number of smooths: Smoothing method: Disabled 3 2 Baselines Join velley threshold: Reduce peek telling: Relee beseline nexlnun: 30.00 70.00 4.00 Peek Seperetlon Drew verticsl threshold: Detect shoulders: Shoulder threshold: , Response Threshold * 10.00 Dlssbled 10.00 ' Relstive height: Absolute height: Relstive eree: Absolute area: 50.00, Enabled 0.00, Disabled 3.00, Enabled 0.00, Disabled LAC o s h u k ` . ' ' Peg. 3 Chad Report Method file: Last modified: Compound: C 1\MASSX,TMx\rt*2.PRO\MathDB\OS2599a.db Rad May 26 07:18:17 19990 6: pros Printed: Rod May 26 08:21:19 1999 Compound Mama: Internal Ref.: Ouantify Trace: Cone, of Standards: pros (None] TIC Cone. A /t/Jte t e w dear RF Value: User Peak Factor: Integrate Mindow rector: 1.000000, Disabled 1.000000 2.000 Peak Location Retention Tina (alna): Relative Retention Tina: Time Ifindo# (mins): 5.281, Enabled 0.000, Disabled 0.293 Peak Selection: Nearest Response - Reeponae Type: Response: External Areas Calibration Curves Polynomial Type: Point of Origin: [ Fit Melghting: Axis Transformation: Linear Exclude l/X None 1 Concentration units: - [ Noise | Paak-to-paak amplitude: 72 Smooth ! Smoothing: ! Window size (scans): i Number of smooths: 1 Smoothing method: . Disabled 3 2 . Baselines j Join valley threshold: , Reduce peak tailing: Raise baseline maximum: 30.00 70.00 4.00 Peek Separation ] Drew vertical threshold: | Detect shoulders: j Shoulder threshold: 10.00 Disabled 10.00 Response Threshold %' | Relative height: 1 Absolute height: 1 Relative area: " Absolute area 2 50.00, Enabled 0.00, Disabled 3.00, Enabled a 0.00, Disabled i V 1 BACK TO MAIN (Jtt osJa l h i_______ Paga A ' Method Report Method file: Last Modified: Compound: Printed: ' C:\MASSLm\rat2.PRO\M*thDB\052S9..mdb Wed Hey 26 07:11:17 19990 S: IS Wed May 26 08:21:19 1999 Compound Name: Internal Ref.: Quantify Trace: Cone, of Standards: User RT Value: User Peak Factor: Integrate Window Factor: Peak Location ' Attention Time (mins) : Relative Retention Tins: Tin* Window (mins) :* Peak Selection: Response - Response Type: Response: Cslibrstion Curves Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation: Concentration units: Noise Peak-to-peak amplitude: Smooth * Smoothing: Window size (scans): Number of smooths: Smoothing method: Baselines loin valley threshold: Reduce peak tailing: Raise baseline maximum: Peak Separation Draw vertical threshold: Detect shoulders: Shoulder threshold: - Response Threshold Relative height: Absolute height: Relative area: Absolute are* ZS (None) TIC Fixed l.OOOOOOp Disabled 1.000000 2.000 5.029, Enabled 0.000, Disabled 0.304 Nearest External Areas `' Linear Exclude 1/X None 83 .Disabled 3 2 30.00 70.00 4.00 10.00 Disabled 10.00 50.00, Enabled 0.00, Disabled 3.00, Enabled 0.00, Disabled . A iJ z l . o u m i BACK TO MAIN Page 5 r  k L te i_____________________ , M assly n x- Sam ple List Sample List C:\MASSLYNX\rats2.PRO\SampleDB\060199c.SPL Printed: Tue Jun 0116:19:18 1999 ' _____ ' BACK TO MAIN - * Pagel F ile Name 1 060199020 2 060199021 3 060199022 4 060199023 5 060199024 6 060199025 7 060199026 8 060199027 9 060199028 10 060199029 11 060199030 12 060199031 13 060199032 14 060199033 15 060199034 16 060199035 17 060199036 18 060199037 19 060199038 20 060199039 21 060199040 22 060199041 23 060199042 24 060199043 25 060199044 26 060199045 27 060199046 28 060199047 29 060199048 30 060199049 31 060199050 32 060199051 33 060199052 34 060199053 35 . 060199054 36 060199055 37 060199056 3389 006600119999005578 40 060199059 41 060199060 42 060199061 43 060199062 44 060199063 45 060199064 46 060199065 47 060199066 48 060199067 49 060199068 50 060199066 51 060199070 52 060199071 53 060199072 54 060199073 55 060199074 56 060199075 57 060199076 58 060199077 59 060199078 60 060199079 ' File Text methanol blank methanol blank tox120, rbl04199-wblk-2 tox120, rbl04199-lblk-2 tox120, rbl04199-5 ppb-2 tox120, rbl04199-10 ppb-2 tox120, rbl04199-25 ppb-2 tox120, rbl04199-50 ppb-2 tox120, rbl041.99-100 ppb-2 tox120, rbl04199-250 ppb-2 tox120, rt>l04199-500 ppb-2 tox120, rbl04199-750 ppb-2 tox120, rbl04199-1000 ppb-2 methanol blank tox120,1M -g1,4/19/99 tox120,2M -g1,4/19/99 tox120,3M -g1,4/19/99 tox120,4M -g1,4/19/99 tox120,5M -g1,4/19/99 tox120, rbl04199-250 ppb-co-1 methanol blank tox120,6M -g1,4/19/99 tox120,7M -g1,4/19/99 tox120,8M -g1,4/19/99 tox120,9M -g1,4/19/99 tox120,10M -g1,4/19/99 tox120, rbl04199-250 ppb-cc-2,5/28/99 methanol blank ' tox120,11M -g1,4/19/99 tox120,13M -g1,4/19/99 tox120,14M -g1,4/19/99 tox120,15M -g1,4/19/99 tox120, rbl04199-250 ppb-cc-3 methanol blank tox120,6M -g1, ms, 4/19/99 tox120,6M -g1, msd, 4/19/99 tox120,13M -g1, ms, 4/19/99 tox120,13M -g1, msd, 4/19/99 tox120, rbl04199-250 ppb-CC-4 methanol blank tox120,3M -g1, ms, 4/19/99 tox120,3M -g1, msd, 4/19/99 tox120,41M -g3,4/19/99, d10 tox120,23M -g2,4/19/99 tox120,24M -g2,4/19/99 tox120, rbl04199-250 ppb-cc-5 methanol blank tox120,25M -g2,4/19/99 tox120,26M -g2,4/19/99 tox120,27M -g2,4/19/99 tox120,29M -g2,4/19/99 tox120,30M -g2,4/19/99 tox120, rbl04199-250 ppb-co-6 methanol blank tox120,31M -g2,4/19/99 tox120,33M -fl3,4/19/99 tox120,34M -g3,4/19/99 tox120,35M -g3,4/19/99 tox120,36M -g3,4/19/99 tox120, rb!04199-250 ppb-cc-1 . MS File 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9 M IN -P O A A -513 -6 1 3 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9 M IN -P O A A -513 -6 1 3 9M IN -P O A A -51 3-61 3 9M IN -P O A A -51 3-61 3 9M IN-POAA-513-613 9M IN-POAA-513-613 99MMIINN--PPOOAAAA--551133--661133 9M IN -P O A A -51 3-61 3 9M IN -P O A A -51 3-61 3 9M IN -P O A A -51 3-61 3 . . 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN -P O A A -51 3-61 3 9 M IN -P O A A -513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M 1N -P O A A -513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3-61 3 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9 M IN -P O A A -513 -6 1 3 9M IN -P O A A -51 3 -6 1 3 In let File 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m ln-2 9m ln-2 9m in-2 , 9min-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 . 9min-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9min-2 9m in-2 9m in-2 9min-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 BACK TO MAIN f U J U s a _______________ Masslynx-Sample List ____________________ _____ :______ :_______________ :____________________ ' ` Page 2 Sam ple L is t C:\M ASSLYNX\rats2PRO\Sam pleDB\060199C.SPL Printed: Tue Jun 01 16:19:181999 ' F ile Nam e 61 060199080 62 060199081 63 060199082 64 060199083 65 060199084 66 060199085 67 060199086 68 060199087 69 060199088 70 060199089 71 060199090 72 060199091 73 060199092 74 060199093 75 060199094 76 060199095 77 060199096 78 060199097 79 060199098 80 060199099 81 060199100 82 060199101 83 060199102 84 060199103 85 060199104 86 060199105 File Text methanol blank 10x120,3 7M -g 3 ,4/19/99 10X120,3 8M -g 3 ,4/19/99 to x 1 20,39M -g 3,4/19/99 1ox120,40M -g3,4/19/99 to x 1 20,41M -g 3,4/19/99 tox120, *104199-250 ppb-cc-2 methanol blank to x 1 20,42M -g 3,4/19/99 to x120,43M -g 3,4/19/99 to x120,45M -g 3,4/19/99 to x120,46M -g 3,4/19/99 to x 1 20,47M -g 3,4/19/99 tox120, *104199-250 ppb-cc-3 methanol blank ' tox120, *l04199-w b lk-2 . . tox120, *1 04 1 99-lblk-2 tox120, *104199-5 ppb-2 tox120, *104199-10 ppb-2 tox120, *104199-25 ppb-2 tox120, *104199-50 ppb-2 tox120, *104199-100 ppb-2 to x l2 0, *104199-250 ppb-2 . tox120, *104199-500 ppb-2 tox120, *104199-750 ppb-2 tox120, *104199-1000 ppb-2 MS File 9M IN-POAA-513-613 . 9M IN-POAA-513-613 9M IN -P O A A -51 3-61 3 9M IN -P O A A -51 3 -6 13 9 M IN -P O A A -513 -6 1 3 9 M IN -P O A A -513 -6 13 . 9MIN-POAA-513-613 9 M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 13 9M IN-POAA-513-613 - 9M IN-POAA-513-613 9 M IN -P O A A -51 3 -6 1 3 9 M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M 1N -P O A A -513-613 9M IN -P O A A -51 3 -6 13 9 M IN -P A A -513 -6 1 3 .. 9MIN-POAA-513-613 9MIN-POAA-513-613 9M IN-POAA-513-613 9 M IN -P O A A -513 -6 1 3 9 M IN -P O A A -513 -6 1 3 9 M IN -P O A A -51 3 -6 1 3 9 M IN -P O A A -513 -6 1 3 9M IN -P O A A -51 3-61 3 In let File 9m ln-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m ln-2. 9m ln-2 9m!n-2 9m ln-2 9m ln-2 9m ln-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m ln-2 BACK TO MAIN 6 I f t f l _________________ _________________________________________________________________________________ Scanning Method Report Vaga 1 M athodi C:\MtSSUrNX\RhTS2.PR0VRCgaDB\9MIM-PaMt-513-S13 Laat Modified: To# JUn 01 3.4:34:06 1999 ' ' Printed: Tua JUn 01 16:19:2 19 ' Solvent Daisy ( nine ) > 0.00 function : 1 M M of 1 Kaaa air ( H P - ) . Inter Channel Delay ( Secs ) : 0.03 Span ( Daltons ) > 0.00 Start Tina { Kins ) i .0.00 End Tina { Mins ) : 9.00 Repeats : 1 Channel Parent Daughter Dwell (Sees) Coll Energy teV) Cone ( V ) 1 413.00 169.00 0.30 20 15 function : 2 M9M of 1 Masa Pair t ESP- I Intar Channel Delay ( Sacs ) > Span ( Daltons ) : Start Tlaa ( Mins I : ' End Tlaa ( Mins ) : Repeats I Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (aV) Cone ( V ) 1 513.00 169.00 0.30 ' 20 15 Function : 3 MOM of 1 Mesa Pair ( ESP- ) .: Inter Channel Delay ( Secs ) I Span ( Daltons ) : Start Tlaa ( Mins ) t End Tlae ( Mins ) t Repeats : Channel Parent Daughter 0.03 o.oo ' ' 0.00 9.00 1 Dwell (Secs) Coll Energy (eV) > Cono ( V ) 1 613.00 169.00 0.30 20 15 Function : 4 M M of 1 Maas Pair ( ESP- ) Inter Channel Delay ( Secs ) : Span ( Daltons ) : Start Tlaa ( Mins ) : End Tlaa ( Mins ) : Repeats : Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (eV) Cone ( V ) BACK TO MAIN (a ( t f a c t _______________ ;____________________________________ -__________________ ____________________ - - Tuning Method Report Method: C :\MASSLYNX\RATS2. P R O \ A C Q O D B \ P F O S Page 1 Printed: Tue Jun 01 16:20:59 1999 MS SOURCE ( ESP- ) Capillary Cone Extractor , RF Lens Source Block Temp. Desolvation Temp. Set Rdbk 3.00 15 5 0.2 150 250 2.97 '-14 0 152 253 Pressures Analyser Vacuum Gas Cell ' Rdbk 1.6e-4 5.1e-3 MSI LM Resolution HM Resolution Ion Energy , Ion Energy Ramp Lens 6 Multiplier .\ MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier Set Rdbk 15.0 15.0^ 2.0 ? . 0.0 56 650 Set Rdbk 15.0 15.0 20 2.0 0.0 40 0 650 21 38 20 -- -- BACKT0 MAIN FACT: DATA REVIEW SUMMARY FORM Date: f j l h c BACK TO MAIN C (~2. l r\ *1 Quantify Conpound S u m u r y Hapert toxlZO, gl-), r U S a n p U Lilt: C :\MA3gLma\rt2.PKO\JuplDBM!f0199<l tt odtfind: Tu* Jun 01 1(:1:01 19* M*thod: C:\MUSLymC\nts2.m0\M*th0t\0019o la.t nodlflad: Wad Jun 02 00:49:03 19 Job Coda: print**: Had Jun 02 07:20:47 1999 Conpound 1: OUA Sanploli.t Taxe l 060199020 athanol blank 2 060199021 ethanol blank 3 060199022 taxl20, rbl019-ublk-2 4 060199023 taxl20, tbl04l-lblk-2 . S 060199024 toxl20, rbl0419*-S ppb-Z 6 060199025 COX120, rbl0419-10 ppb-2 ? 060199026 toxl20, rbl04199-25 ppb-2 . 8 06019902? toxl20, rbl04199-50 ppb-2 9 060199028 COX120, rbl04199-100 ppb-2 10 060199029 toxl20, rb104199-250 ppb-2 11 060199030 toxlZO, rbl04199-500 ppb-2 12 060199031 toxlZO, rbl04199-750 ppb-2 13 060199032 toxl20. rbl04199-1000 ppb-2 14 060199033 Mthanel blank 15 060199034 toxl20, lK-gl. 4/19/99 16 060199035 toxl20, 2H-*1, 4/19/99 1? 06019903 toxl20, 3M-gl. 4/19/99 . 19 060199037 toxl20, H-gl, 4/19/99 19 060199038 toxl20, SM-gl, 4/19/99 20 060199039 tOX120, rb!04199-250 ppb-ee-1 21 060199040 athanol blank 22 060199041 tox!20, SM-gl. 4/19/99 23 060199042 toxl20, 7M-gl, 4/19/99 24 060199043 toxI20, 9H-gl, 4/19/99 25 060199044 toxl20, 9M-gl, 4/19/99 26 060199Q4S toxl20, 10H-gl, 4/19/99 27 060199046 toxl20, rbl04199-250 ppb-cc-2, 5/29/99 28 060199047 athanol blank 29 060199049 toxl20, HM-gl, 4/19/99 30 060199049 toxl20, 13M-gl, 4/19/99 31 060199050 toxl20, 14N-gl, 4/19/99 32 060199051 - toxl20, ISM-gl. 4/19/99 33 060199052 toxl20, rbl04199-250 ppb-ec-3 34 0601990S3 ethanol blank 35 060199054 toxl20, SM-gl, ma, 4/19/99 36 060199055 toxl20, (M-gl, mad, 4/19/99 37 060199056 toxl20, 13H-gl, ma, 4/19/99 38 060199057 toxl20, 13M-gl. mad, 4/19/99 39 060199058 toxl20, rbl04199-250 ppb-cc-4 40 060199059 41 060199060 athanol blank toxl20, 3M-gl, ma, 4/19/99 42 060199061 toxl20, 3M-gl, u d , 4/19/99 43 060199062 toxl20, 41N-g), 4/19/99, dlO 44 060199063 toxl20, 23M-g2, 4/19/99 45 060199064 toxlZO, 24M-g2, 4/19/99 46 060199065 toxl20, rbl04199-250 ppb-cc-S 47 060199066 athanol blank 48 060199067 toxl20, 25M-g2. 4/19/99 49 060199068 toxl20, 2<M-gZ. 4/19/99 ' 50 060199069 toxl20, 21M-2, 4/19/99 51 060199070 toxl20, 29H-g2, 4/19/99 52 060199071 toxl20; 30M-g2, 4/19/99 53 060199072 toxl20, rbl04199-250 ppb-cc-( 54 060199073 athanol blank 55 060199074 toxl20, 31M-2, 4/19/99 56 060199075 COX120. 33K--g3, 4/39/99 57 060199076 toxl20, 34H-g3, 4/19/99 58 060199077 ' toxl20, 35M-g3, 4/19/99 59 060199078 toxl20, 36M-g3, 4/19/99 60 060199079 toxl20, rbl04199-250 ppb-ee-1 61 060199080 athanol blank . 62 060199081 toxlZO, 37M-g3, 4/19/99 63 060199082 toxl20, 3H-g3, 4/19/99 64 060199083 tox!20, 39M-g3, 4/19/99 5 060199084 toxl20. 40M-g3, 4/19/99 66 060199085 . toxl20. 41tl-g3, 4/19/99 67 060199086 toxl20, rbl04199-250 ppb-cc-2 68 060199087 athanol blank <9 060199088 toxl20, 42M-3, 4/19/99 70 060199089 toxl20, 43M-3, 4/19/99 ?l 060199090 toxl20, 45M-g3, 4/19/99 72 060199091 toxl20, 4(X-3, 4/19/99 73 060199092 toxl20, 41H-g3, 4/19/99 74 060199093 toxl20, rbl04l99-250 ppb-ec-3 ?5 060199094 ethanol blank 76 060199095 toxl20, rb!04199-ubl k-2 77 060199096 toxHO, rbl04199-lblk-2 78 060199097 toxlZO, rbl04199-5 ppb-2 79 060199098 toxlZO, rbl04199-10 ppb-2 80 060199099 t0x120, rbl04l99-2S ppb-2 81 060199100 tox!20, rbl04199-50 ppb-2 2 060199101 0OX120, rbl04199-100 ppb-2 83 060199102 toxl20, rb!04199-2S0 ppb-2 84 060199103 toxl20, rbl04199-500 ppb-2 85 060199104 toxl20, rbl04199-750 ppb-2 86 060199105 toxl20, rb!04199-1000 ppb-2 . Typo Analyt* Analyte Analyte Analyte Standard Standard Standard Standard Standard Standard Standard .Standard Standard Analyte Analyte Analyte Analyte Analyte Analyt* QC Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte ' Analyte Analyte QC Analyte Analyt* Analyte Analyte Analyt* Analyt* QC Analyt* Analyt* Analyt* Analyt* Analyt* Analyt* QC Analyt* Analyt* Analyt* Analyt* Analyt* Analyt* QC Analyt* Analyt* Analyt* Analyt* Analyt* Analyt* QC Analyt* Analyt* Analyt* Standard Standard Standard Standard Standard Standard Standard Standard Standard ltd Cone 5.56 11.10 27.80 55.60 111.00 278.00 556.00 33.00 1111.00 * 278.00 , 278.00 278*00 278.00 278.00 270.00 278.00 278.00 271.00 5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111*00 W 5.082 5.445 4.974 5.052 4.925 5.046 5.038 5.027 5.033 5.020 5.033 5.031 4.978 5.051 4.919 5.034 5.030 5.205 4.730 5.008 5.054 4.989 5.026 4.645 5.435 5.288 4.586 5.033 5.048 5.038 5.050 5.036 5.052 5.287 5.044 5.031 5.049 5.029 5.031 5.039 5.044 5.029 5.046 5.001 5.019 5.021 5.040 5.047 5.032 5.046 5.017 5.025 5.046 5.068 S.0S3 5.049 5.047 5.046 5.033 5.042 5.039 5.049 5.033 5.046 5.031 5.040 5.043 5.077 5.040 5.067 5.063 5.042 5.021 5.025 5.045 5.022 5.025 5.027 ATM 4 Cona. riftai 0.00 bb 3 12 19 9 34 27 65 114 203 280 351 3 5 0.00 bb 24.56 bb 43.28 M4X 12.72 MM 98.26 MMX 69.51 bb 193.86 bb 329.66 bb 599.36 bb 831.57 bb 1046.72 bb 0.0O bb 2.11 bb 678 15 217 25 66 19 8 0 -6 3 0.00 bb 9 14.05 MC 105 303.16 bb 9 4 0.00 bb 6 6.49 bb 9 15.27 MM 6 6.15 MM 5 2.55 HM 110 317.79 bb 2 0.00 bb 9 12.89 bb 7 8.74 bb 14 2 0.00 bb 102 294.80 bb 6 84 84 111 103 112 3 98 90 163 1396 1984 103 239.96 bb 240.30 bb 320.54 bb 299.29 KM 324.29 bb 0.00 bb 281.95 MM 281.92 bb 477.77 bb 4201.20 MM 5974.35 bb 298.39 MM 17 7 995 1026 1442 3S8 424 97 5 512 1365 1788 1658 1508 109 *9 v 1827 1927 1514 1952 1018 109 11 395 907 325 259 423 112 2989.84 MM 3084.63 bb 4338.59 MM 1068.17 bb 1266.11 bb 281.06 bb 0.73 bb 1532.20 bb 4105.96 bb 5384.18 bb 4989.13 bb 4537.13 bb 314.53 bb 13.57 bb 5500.87 bb 5802.57 bb 4556.28 to 5879.27 to 3050.38 bb 314.64 bb 18.85 to 1180.46 bb 2723,34 bb 967.37 bb 768.44 bb 1263.23 bb 325.47 bb ' r* l 13 13 17 10 18.52 bb 15 33.16 bb 20 47.92 bbX 2 0.00 bb 33 8.03 bbX 27 7.89 MM 48 132.00 MM 98 281.90 HM 196 578.50 bb 256 758.81 bb 344 1025,06 MM 762 -100 217 22 19 1 4 -9 - dalia 0(2490: quantiatad and printad by ko) */02/f* i sis n s " - h SS G 2*nS m ai "SSSSS 22 5 S 5 2 3 * iiiiii 2SS5 1 5 S * 333 S 22222SR22S5 22SS2S S5PZSK 2828CK SSS?P2 1 iiiii HHii iiiiii iiiiii miii iiiiii 2 2 2 2 2 2 2 2 2 2 2 2 S 2 S 2 2 S USSKSS 888X82 2 2 2 2 2 2 RIl; ? 3IrtS* 3 SS S S gS P =iUii - "223SS S 2522? 5SSSSS 552322 2S2522 585253 2 5 5 5 2 S w in io *o * m m o m m*m n n i i n *o n m **o *o immninuv S 355522 sS2 5ii S s s SSSSSSooo S 5 5 "=35333 Aneli 062490: quantiiUd end printed by he) C/02/99 ri ll lll!ll ll!l l!iilll!illl!illllllllillill!llllili !lill!l ill ll illllllli lllll l i ''-'S222SSS2S2aSH2SSS222SSH2S3SKS2S233533255i8KaaSSSSSSSS32S3S25S2PPP?P2P?22S2SSSS BACK TO MAIN Quantify Compound Summary lUport toxl20, gl-3, rtl Sampl U t : C:\MASL7NX\rt*2.W0\S**loD\060199c U a t nodlflad: Tu Jun 01 U : 18:01 1999 Mathod: C:\M*SSLTNX\rCj2.WO\MthBB\06019#a Laat nodifiad: W*d Jun 02 09:48:03 1999 Job Cod: mnttd: *d Jun 02 07:20:47 1999 * Compound 3: M613 f Nam laaplaUit T u t 1 060199020 Chino1 blank 2 060199021 Mthanol blank 3 060199022 toxl20, rbl04199-wblk>2 4 060199023 Coxl20, rbl0419S-lblk-2 5 060199024 toxl20, rbl04199-5 ppb-2 6 060199025 t0x120 rbl04199-10 ppb-2 7 060199026 COX120, rb!04199-25 ppb-2 8 060199027 COX120, rbl04199-50 ppb-2 9 060199028 COX120, rbl04199-100 ppb-2 10 060199029 oxl20, rbl04199-250 ppb-2 '' U 060199030 toxl20, rbl04199-500 ppb-2 12 060199031 toxl20, rbl04199-750 ppb-2 13 060199032 toxl20, rbl04199-1000 ppb-2 ' 14 060199033 Mthanol blank 15 060199034 COX120, IM-gl, 4/19/99 16 060199035 toxl20, 2M-gl, 4/19/99 17 060199036 toxl20. 3H-gl, 4/19/99. ' 18 060199037 0X120* 4H-1, 4/19/99 19 060199038 COX120* SK-gl, 4/19/99 20 060199039 toxl20. rbl04199-250 ppb-cc-1 21 060199040 Mthanol blank 22 060199041 0x120, St-gl, 4/19/99 23 060199042 oxl20, 7M-gl, 4/19/99 24 060199043 toxl20, 6H-gl, 4/19/99 25 060199044 0X120, 9K~gl, 4/19/99 26 060199045 toxl20, 10M-gl, 4/19/99 27 060199046 oxl20, rbl04199-2S0 ppb-ec-2. 5/21/99 28 060199047 aChanol blank 29 060199048 toxl20, llM-gl, 4/19/99 30 060199049 toxl20, 13M-gl, 4/19/99 31 060199050 0X120, 14N-gl, 4/19/99 32 060199051 COX120, ISH-gl, 4/19/99 33 060199052 toxl20, rbl04199-250 ppb-cc-3 34 060199053 Mthanol blank 35 060199054 toxl20, CM-gl, m m . 4/19/99 36 060199055 toxl20, H-gl, u d , 4/19/99 37 060199056 toxl20, 15M-gl, M , 4/19/99 38 060199057 toxl20, 15M-gl, u d , 4/19/99 39 060199058 COX120, rbl04199-250 ppb-cc-4 40 060199059 Mthanol blank 41 060199060 COX120, 3H-gl, M , 4/19/99 42 060199061 toxl20, 3M-gl, u d , 4/19/99 43 060199062 toxl20, 41N-g3, 4/19/99, dlO 44 060199063 toxl20, 23K-g2, 4/19/99 45 060199064 toxl20, 24M-g2, 4/19/99 46 060199065 Coxl20, rbl04199-250 ppb-cc-5 . 47 060199066 ebano1 blank 48 060199067 0X120, 25M-g2, 4/19/99 49 060199068 tox!20, 2H-g2, 4/19/99 50 060199069 toxl20, 27H-g2, 4/19/99 51 060199070 52 060199071 toxl20, 29H-g2, 4/19/99 0X120, 30H-g2, 4/19/99 ' 53 060199072 54 060199073 0X120, rbl04199-250 ppb-cc-6 Mthanol blank 55 060199074 toxl20, 31M-g2, 4/19/99 56 060199075 CoxlZO. 33K-g3, 4/19/99 57 060199076 . toxl20, 34M-g3, 4/19/99 58 060199077 toxl20, 3SN-g3, 4/19/99 59 060199078 COX120, 3<M-g3, 4/19/99 60 060199079 COX120, rbl04199-250 ppb-cc-1 61 060199080 Mthanol blank - 62 060199081 toxl20, 37N-03, 4/19/99 63 060199082 0X120, 3IN-g3, 4/19/99 64 060199083 COX120, 39M-g3, 4/19/99 65 060199084 0X120, 40M-g3, 4/19/99 66 060199085 toxl20, 41M-g3, 4/19/99 67 060199086 oxl20, rbl04199-250 ppb-ce-2 68 060199087 Mthanol blank 69 060199088 0X120, 42H-g3, 4/19/99 70 060199089 toxl20, 43M-gl, 4/19/99 71 060199090 toxl20, 45H-g3, 4/19/99 72 060199091 toxl20, 4<H-g3, 4/19/99 73 060199092 COX12, 47M-g3, 4/19/99 74 060199093 toxl20, rbl04199-2S0 ppb-cc-3 75 060199094 Mthanol blank 76 060199095 0x120. rbl04199-wblk-2 77 060199096 0X120, rbl04199-lblk-2 78 060199097 . toxl20. rb!04199-5 ppb-2 79 060199098 toxl20, rbl04199-10 ppb-2 80 060199099 oxl20, rbl04199-25 ppb-2 81 060199100 COX120, rbl04199-50 ppb-2 82 060199101 COX120, rbl04199-100 ppb-2 83 060199102 COX120, rb!04199-250 ppb-2 84 060199103 tox!20, rbl04199-500 ppb-2 . 85 060199104 COX120, rb!04199-750 ppb-2 86 060199105 COX120, rbl04199-1000 ppb-2 ' *yp* Std Cono ' Analyta Analyta Analyta Analyto Standard 5.56 Standard 11.10 Standard 27.80 Standard 55.60 Standard 111.00 Standard 278.00 Standard 556.00 ' *Standard 33.00 Standard 1111.00 Analyta Analyta Analyta Analyta Analyta ' Analyta QC 270.00 Analyta Analyta Analyta Analyta Analyta Analyta QC 270.00 Analyta Analyta Analyta Analyto Analyta QC 278.00 Analyto Analyta / Analyta Analyta Analyta QC 278.00 Analyta Analyta Analyte . Analyta Analyta Analyta QC 278.00 Analyta Analyta Analyta Analyta Analyta Analyta QC 278.00 Analyta Analyta Analyta Analyta Analyta Analyta QC 278.00 Analyta Analyta Analyta Analyta Analyto Analyta QC 270.00 Analyta Analyta Analyta Analyta Analyta Analyta QC 278.00 Analyta Analyta Analyta Standard 5.56 Standard 11.10 Standard 27.80 Standard Standard 55.60 111.00 Standard 278.00 Standard 556.00 Standard 833.00 Standard 1111.00 *T 5.743 5.791 5.820 5.798 5.769 5.793 5.700 5.771 5.770 5.773 5,793 * 5.779 5.790 5.783 5.791 5.804 5.798 5.790 5.799 5.797 5.777 5.786 5.010 3.797 5.792 5.760 5.784 5.784 5.813 5.785 5.770 5.806 5.790 5.584 3.796 5.812 5.000 5,790 3.790 5.767 Arm Cono. T l m g m tOv 3 36.00 bb 14 154.82 bbX 9 102.31 bbX 4 40.84 MM 10 112.24 bb 21 238,05 bb 53 588.77 MM 81 889.42 MC 91 1009.33 MCX 2684 822 -27 1 -14 6 7 -9 21 236.61 bb -15 20 226.25 bb It 10 204.21 MM -27 15 163.64 bb 17 185.98 bb 20 223.84 bb 21 231.57 bb 22 238.60 bb -14 22 240.26 bb 21 228.26 bb 9 96.59 MM 10 112.16 bb . 20 223.25 HK -20 3 61.02 MM 11 117.93 MM 13 141.67 bb 10 112.36 MM 8 86.01 MM 25 272.03 MM 11 11729 bb , -2 5 60.98 bb . 25 277.42 MM 0 23 252.51 MM -9 20 219.11 bb 9 103.83 bb -21 5 51.85 MM 13 171.76 MM 22 243.17 MM 48 533.24 MM 72 797.88 MM 01 092.89 MMX -7 55 -13 -4 -4 -20 Pag 3 A m i l a 082498: quanelataci and printed fay Ko) 4/02/99 BACK TO MAIN Quantify Confound Summary Raport toxl20, gl-3, rtl Sampla U t ! C:\MMSLTHX\rata2.PRO\SMgilaOa\OS019flo Laat modifiad: Tu* Jun 01 16:18:01 1999 Mathod: C:\HASSLYHX\rata2.PTO\MathOS\0C0199a Laat modifiad: Nad Jun 02 04:49:03 1999 Job Coda: Printad: Nad Jun 02 07:20:4? 1999 _ Coanpound 4: 18 Mama *" 1 060199020 2 060199021 3 060199022 4 060199023 3 060199024 6 060199025 7 060199026 8 060199027 9 060199020 10 060199029 11 060199030 12 060199031 13 060199032 14 060199033 15 060199034 16 060199035 17 060199036 10 060199037 19 060199038 20 060199039 21 060199040 22 060199041 23 060199042 24 060199043 25 060199044 . 26 060199045 2? 060199046 28 060199047 29 060199048 30 060199049 31 060199050 32 060199051 33 060199052 34 060199053 35 060199054 36 060199055 3? 060199056 38 060199057 39 060199058 40 060199059 41 060199060 42 060199061 43 060199062 44 060199063 45 060199064 46 060199065 47 060199066 48 06019906? 49 060199068 50 060199069 51 060199070 52 060199071 53 060199072 54 060199073 55 060199074 56 0 6 0 1 9 9 0 7 5 57 060199076 58 060199077 59 060199078 60 060199079 61 060199080 62 060199081 63 060199082 64 060199083 65 060199084 66 060199085 67 0601990B6 68 060199087 69 060199088 70 060199089 71 060199090 72 060199091 73 060199092 74 060199093 75 060199094 76 060199095 77 060199096 78 060199097 79 060199098 80 060199099 01 060199100 82 060199101 83 060199102 84 060199103 05 060199104 86 060199105 Saaplaliat Taxt mathanol blank mat h a n d blank toxl20, rbl04l99-wblk-2 toxl20, rb!04199-lblk-2 toxl20, rbl04199-5 ppb-2 toxl20f rbi04199-10 ppb-2 toxl20, rbl04199-2S ppb-2 toxl20, rb!04199-50 ppb-2 toxl20, rbl04199-100 ppb-2 toxl20, rbl04199-2S0 ppb-2 * toxl20, rbl04199-500 ppb-2 ' toxl20, rbl04199-750 ppb-2 toxl20, rbl04199-1000 ppb-2 ' mathanol blank toxl20, IK-gl, 4/19/99 toxl20, 2M-gl, 4/19/99 toxl20, 3M-gl, 4/19/99 . * tOX120, 4H-gl, 4/19/99 tOX120, SM-gl, 4/19/99 COX120, rbl04199-250 ppb-ee-1 mathanol blank tox!20, 6M-gl, 4/19/99 toxl20, 7K-gl, 4/19/99 toxl20, 8M-gl, 4/19/99 tox!20, 9M-gl, 4/19/99 toxl20, 10M-gl, 4/19/99 toxl20, rbl04199-250 ppb-cc-2, 5/28/99 mathanol blank toxl20, llM-gl, 4/19/99 toxl20, 13H-gl, 4/19/99 tox!20, 14M-gl, 4/19/99 * toxl20, 15M-gl, 4/19/99 tox!20, rbl04199-250 ppb-cc-3 mathanol blank toxl20, 6M-gl, ma, 4/19/99 toxl20, 6H-gl, mad, 4/19/99 tox!20, 13M-gl, ma, 4/19/99 toxl20, 13H-gl, mad, 4/19/99 toxl20, rbl04199-250 ppb-cc-4 mathanol blank taxl20, 3M-gl, ms, 4/19/99 toxl20, 3M-gl, mad, 4/19/99 tox!20. 41M-g3, 4/19/99, dlO toxl20, 23M-g2, 4/19/99 toxl20, 24H-g2, 4/19/99 toxl20, rbl04199-250 ppb-cc-5 mathanol blank tox!20, 25H-g2, 4/19/99 toxl20, 26M-g2, 4/19/99 toxl20, 27M-g2, 4/19/99 toxl20* 29M-g2, 4/19/99 toxl20, 30K-g2, 4/19/99 toxl20, rbl04199-250 ppb-cc-6 mathanol blank toxl20, 31H-g2, 4/19/99 COX120, 33H -g3, 4/19/99 toxl20, 34M-g3, 4/19/99 toxl20, 35H-g3# 4/19/99 toxl20, 36M-g3, 4/19/99 toxl20, rbl04199-250 ppb-cc-1 mathanol blank * toxl20, 37H-g3, 4/19/99 toxl20, 38M-g3, 4/19/99 toxl20, 39H-g3, 4/19/99 toxl20, 40H-g3, 4/19/99 toxl20, 41M-g3, 4/19/99 toxl20, rbl04199-2S0 ppb-cc-2 mathanol blank tox!20, 42H-g3, 4/19/99 toxl20, 43M-g3, 4/19/99 tOXl20, 45M-g3, 4/19/99 tOx!20, 46M-g3, 4/19/99 toxl20, 47M-g3, 4/19/99 toxl20f rbl04199-250 ppb-cc-3 mathanol blank tox!20, rbl04199-wblk-2 toxl20, rbl04199-lblk-2 toxl20, rbl04199-5 ppb-2 toxl20* rbl04199-10 ppb-2 toxl20, rbl04199-25 ppb-2 toxl20, rbl04199-50 ppb-2 Cox120, rbl04199-100 ppb-2 COX120, rbl04199-250 ppb-2 toxl20# rbl04199-500 ppb-2 toxl20, rb!04199-750 ppb-2 toxl20, rb!04199-1000 ppb-2 Saga 4 ' * typ Analyt. Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard `Standard Standard Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta ac Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Std Cono 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 . 1.00 ' 1.00 1.00 1.00 1.00 1.00 1 a 00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 l.OO ** 5.061 5.05? 5.024 5.031 5.018 5.032 5.021 5.027 5.014 5.026 5.003 5.024 5.016 5.046 5.028 5.010 5.024 5.023 5.014 5.024 5.004 5.005 5.021 5.028 5.014 5.023 5.023 5.027 $.021 5.033 5.023 5.030 5.024 5.016 5.025 5.022 5.023 5.025 5.033 5.016 5.024 5.018 4.996 5.013 5.015 5.042 5.004 5.019 5.012 5.019 5.018 5.025 5.021 5.042 5.019 5.005 5.014 5.022 5.049 5.018 5.026 5.033 5.015 5.050 5.034 5.039 5.036 5.037 5.023 4.997 5.018 5.016 5.020 4.999 Arm* Cone. rlmg %Dv 862 1055 1051 1057 1119 909 1047 906 907 898 972 779 873 72 909 764 70 691 730 809 700 870 953 702 684 783 667 963 768 763 696 810 948 036 877 93 466 555 886 541 523 1219 779 536 895 591 90S 509 . 457 546 ,.Nv ! 517 483 630 466 471 940 659 566 535 615 534 136 913 923 923 974 1061 08 978 843 750 764 050 0.92 bb 1.13 bb 1.12 bb 1.13 bb 1.20 bb 0.97 bb 1.12 bb 0.97 bb 0.97 bb 0.96 bb 1.04 bb -8 13 12 13 20 -3 12 -3 -3 -4 4 0.83 bb 0.93 bb 0.93 bb 0.97 bb 0.82 bb 0.93 bb -17 -7 -7 -3 -18 . -7 0.74 bb 0.7* bb 0.87 bb 0.75 bb 0.93 bb 1.02 bb -26 -22 -13 -25 -7 2 0.75 bb 0.73 bb 0.84 bb 0.71 bb 1.03 bb -25 -27 -16 -29 3 0.82 bb 0.82 bb 0.74 bb 0.87 bb 1.01 bb -18 -1B -26 -13 1 0.89 bb 0.94 bb 0.10 bb 0.50 bb 0.59 bb 0.95 bb -11 -6 -90 -50 * -41 -5 0.58 bb 0.56 bb 1.30 MH 0.83 bb 0.57 bb 0.96 bb 0.63 bb 1.05 bb 0.54 bb 0.49 bb 0.50 bb 0.87 bb -42 -44 30* -17 -43 -4 -37 5 -46 -51 -42 -13 0.55 bb 0.52 bb 0.67 bb 0.50 bb 0.50 bb 1.01 bb -45 -48 -33 -50 -50 1 0.71 bb 0.61 bb 0.57 bb 0.66 bb 0.57 bb 0.89 bb -29 -39. -43 -34 -43 -11 0.90 bb 0.99 bb 0.99 bb 1.04 bb 1.14 bb 0.06 bb 1.05 bb 0.90 bb 0.81 MM 0.82 bb 0.91 MM -2 -1 -1 4 14 -14 5 -10 -19 -18 -9 Amalia 042498: quantiatad and printad by hoj 4/02/99 BACK TO MAIN <o f e A ________________________________________'_____________ _ _ _ _________________________ _ Quantify Calibration Itaport toxl20, 1-3, rtl Vmq* 1 Calibration: C:\HM2LYNX\rata2.PM\Ciirvo09\090199a Loot nodlflod: Usd JUn 02 07:10:00 1000 Prlntod: Wod Jun 02 07:20:01 1999 . * Inolia 002490: quantlatad and prlntod b y hoJ 0/02/90 Quanti fy Callbration Xaport toxl20, gl-3, ttl callbration : C :\MM0lYMC\rata2.M0\CurvoDB\0019e Laat aodiflad: ad Jun 02 07)10:0 loto rrintad: ad Jun 02 07:20:51 1900 BACK TO MAIN : Quantiiy Calibratlon m o r e toxlJO, gl-2. rtl Calibratlon: C:\HhoaLTMX\raea2.mo\airvaDa\040199a taat aodlfiad: ad Jun 02 07:10:04 1290 Prlntad: Wad Jun 02 07:20:31 1992 ' BACK TO MAIN . p9 4 * a U a 042490: quantlatad and prlntad by ho) 4/02/99 SOUP 020199: FA CT E quipm ent Log 99014 Q uattro II, #6414E PAGE DATE: 06/08/99 ANALYST: drb STUDY NUMBER:TOX-120 M ATRIXrRat Liver DESCRIPTION: POAA analog check FACT M ethod: ETS-8-5.0 . Injection volume: 10 uL Cycle time: 9 min. Electrode: Z-Sprav______ MobUe Phase: (A)2.0 mM NH4CH3C02 (B) MeOH ___________ T N -A -orstndrd num ber (A) 99012-10-9________ ffiYTN-A-2540_________ Prepared (date/by): E x p ires: /A) 06/04/99 drb (A) 10/99______________ ffi) 06/03/99 drb (B) 10/99______________ Store at room tem p? ffj or N O th er_______ ;__________________ G uard colum n: None__________________ Part number: na_________ Lot num ber na________________________ A nalytical colum n: Keystone B etasil_________________________ '___________ Size: 2 x 50 mm________________ Type: C l 8____________ Sphere size: 5 urn _______ P art number: 055-701-2__________ Lot number P7LQ4 Serial number:0481175Z A nalytical p aram eters: Drying gas (L /h r ) :J 5 Q _ ESI nebulizing gas OJbr) 15 LC Flow rate: 300 nTVmin Back pressure: 55 b ar Sheath gas (L/br)_0____ . H PLC solvent g rad ien t: 9m in-2 Tim e fmin.1 DJOLQQ__________ 2 ) 1.00 3) 4.50 4) JL5Q______ 517.00________ %B 4 0 .0 4 0 .0 9 5 .0 210. 40.0_ i M asslynx - Sam ple List Sample List: C:\MASSLYNX\soup-rats.pro\SampleDB\060899a.SPL Printed: Tue Jun 0815:56:341999 BACK TO MAIN Page 1 File Name 1 060899001 2 060899002 3 060899003 4 060899004 5 060899005 6 060899006 7 060899007 8 060899008 9 060899009 R ie Text M S F ile In le t R le B ottle MeOH Blank . Tox-120, RBL04199-UverBlk-1,4 /1 9 /! Tox-120, RBL04199-10 ppb -1 ,4 /1 9/S Tox-120, RBL04199-100 ppb -1 ,4 /1 9 , Tox-120, RBL04199-1000 ppb -1 ,4 /1 ! MeOH Blank TO X -120,19M G2 MeOH Blank T O X -120,35M G 3 POAA-51: POAA-51! POAA-51: POAA-51: POAA-51: POAA-51: POAA-51: POAA-51: POAA-51: 9m in-2 9m in-2 9m in-2 9m in-2 9m In-2 9m ln-2 9m in-2 9m in-2 9m in-2 91 1 2 3 4 91 5 92 6 4 V olum e S a m p le T C one A 10 Analyte 10 Analyte 10 Analyte 10 10 Standard 100 10 Standard 1000 10 Analyte 10 Analyte ' 10 Analyte 10 Analyte i Acumina Method Report - uthedi ci\KMSLnxNAODr>UTa.movacgin\peAA-5l3-(i3 M a t Modifiedi Tua Jun 0* 13:55>54 U M Printed! Sua Jun 0( 15i00>4 1*3 folvant Delay ( mina ) t 0.00 ' Fonction t 1 KAM of 1 Kaaa Fair ( W - ) Inter channel Dalay ( Seca ) i Span ( Daltons ) i Start Tina ( Mins ) Snd Tina ( Mina ) i Repasta Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Seca) Coll Energy (eV) Cone ( V ) 1 413.00 103.00 '0.25 20 IS Function i 2 MAM of 1 Maas air ( RAF- ) Inter Channel Delay ( Sec* ) > Span { Dalton* ) t Start Time ( Kins ) < * End Tima ( Kin* ) i Repeats t Channel Parent Daughter 0.03 0a00 0.00 9.00 * 1 Dwell (Secs) Coll Energy (eV) Cono ( V ) 1 513.00 169.00 0.25 20 ' Function t 1 MRM of 1 ** Fair { MSP- ) Intar Channel Dalay ( Secs ) > Span ( Daltons ) Start Tine ( Mins ) i Snd Tine ( Mins ) > Repeats > Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (eV) Cone ( V ) 1 513.00 159.00 0.25 20 15 Function t 4 M m of 1 M a s Fair ( BSP- ) Inter Channel Delay ( Saca ) i Spaa ( Daltons ) i Start Tine ( Mina ) t Snd Tine ( Mina ) i Repaata t Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) Coll Energy (eV) Cone ( V. ) 1 353.00 119.00 0.20 20 15 Function t S M m of 1 Naan Fair ( BSP- ) Inter Channel Dalay ( Sec* ) i Span ( Daltons ) i Start. Tine ( Mina ) i Bnd Tine ( Mina ) i Repeats t Channel Parent Daughter 0.03 0.00 0.00 9.00 1 Dwell (Secs) 1 453.00 169.00 0.20 20 M (eV) Cone ( V ) IS Function 1 4 M m if 1 Maas Fair ( BSP- ) Inter channel Delay { Secs ) i span { Daltons ) i Start Tine ( Mins ) > Snd Tine ( Mina ) i Repeats i Channel parent Daughter 0.03 0.00 0.00 9.00 1 H 2 (Secs) Coll Energy (eV) Cone ( V ) 1 553.00 169.00 . 0.20 20 15 notion 7 MAM et 1 Masa Fair l rat- ) Inter Channel Delay ( Saca ) Span ( Daltons ) i start Tine ( Mina > tnd Tina ( Mins ) > Repeats i 0.03 0.00 o.OO j.OO 1 Channel Parent Daughter Dwell (Seca) Coll energy (eV) 1 553.00 159.00 0.20 ' 20 Cone { V ) 15 BACK TO MAIN .0 1 Tuning Method Report - Method: C :\MASSLYNX\SOUP-RATS. PR O\ACQUDB\PFOS Printed: Tue Jun 08 16:43:16 1999 BACK TO MAIN O b /D & f Page 1 SOURCE ( ESP- ) 1 Capillary j Cone Extractor . RF Lens Source Block Temp. 1 Desolvation Temp. 1 1 | Pressures . 1 Analyser Vacuum 1. ] Gas Cell Set Rdbk 3.00 60 5 0.2 150 250 3.01 -60 -7 153 252 Rdbk 2.2e-4 4.9e-3 ' MSI LM Resolution HM Resolution ion Energy Ion Energy Ramp Lens 6 Multiplier ,\ MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ran Lens 8 Lens 9 Multiplier Set Rdbk 14.0 14.0 2.0 0.0 52 650 Set Rdbk 15.0 15.0 45 2.0 0.0 40 0 650 43 38 43 AMELIA062498: FACT Equipment Log 99016 Q uattro H , #6448E PAGE DATE: 06/09/99 ANALYST: sah/mee STUDY NUMBER Tox-120, MATRIX: rat liver DESCRIPTION: IP FACT Method: cts-M -5.0________ Injection volume. lOuL Cycle tim e: 13.5 min. Electrode: Z-Spray M obile Phase: (A)2.0 mM NH4CH3CQ2 (B) MeOH TN-A- or stndid num ber (A ) 99012-10-6________ (B) TN-A-2647 Prepared (date/by): (A) 5/21/99 ias__________ (B) 6/7/99 sah Expires: (A) 11/99_______________ (B) 12/99________ Store at room temp? [7] or N O ther: G uard colum n: None___________________ Part num ber n a ________ Lot number na A nalytical colum n: keystone betasil____________ _ Size: 2 . x 100mm>' " Type: cl8 Sphere size:5 um . Part num ber 105-705-2______ Lot num ber p7h!2 Serial num ber 068640b A nalytical param eters: Drying gas (L/hr): 300 ESI nebulizing gas (L/hr) 15 LC Flow rate: 300 uL/min. Back pressure: 80 bar Sheath gas (L/hr)J) H FLC solvent g radient: p fb sl Tim e (m in.) )1 0 . 0 0 )2 1 . 0 3 8.5 5) 1 1 . 0 )6 1 2 . 0 . %B 5 5 90 90 5 BACK TO MAIN /ngg -- A ffo Hfum JM IMJs i M asslynx - Sam ple U s t - Sample Ust: C:\MASSLYNX\ameOa'rats.PRO\SampleOB\06999a.SPL Printed: W ed Jun 0 915:01:191999 File Nam e File Text 1 060999001 methanol blank ' 2 060999002 methanol blank 3 060999003 tox120, rt02229-H2Oblk-1 4 060999004 tox120, rbl02229-Livertolk-1 5 060999005 tox120, rbi02229-5ppb-1 6 060999006 tox120, rbl02229-10ppb-1 7 060999007 tox120, rbl02229-25ppb-1 8 060999008 tox120, rbl02229-50ppb-1 9 060999009 tox120, rbl02229-100ppb-1 10 060999010 tox120, rt>l02229-250ppb-1 11 060999011 tox120. rbl02229-500ppb-1 12 060999012 tox120, rbl02229-750ppb-1 13 060999013 tox120, rbl02229-1000ppb-1 14 060999014 methanol blank 15 060999015 to x 1 20 ,106F-G 1-250ppb-ms, 2/22/99 16 060999016 to x 1 20 ,106F-G1-250ppb-msd, 2/22/99 17 060999017 tox120,3M -G1-250ppb-m s, 4/19/99 18 060999018 tox120,3M -G1'250ppb-m sd, 4/19/99 19 060999019 tox120, rbl02229-250ppb-cc1 20 060999020 methanol blank 21 060999021 tox120,153F-G 4,2/22/99 22 060999022 tox120,154F-G 4,2/22/99 23 060999023 tox120,155F-G 4,2/22/99 24 060999024 tox120, rbl02229-250ppb-cc2 25 060999025 methanol blank - 26 060999026 to x120,157F-G 4,2/22/99 27 060999027 tox120,158F-G 4,2/22/99 28 060999028 tox120,159F-G 4,2/22/99 - 29 060999029 tox120, rbl02229-250ppb-cc3 30 060999030 methanol blank 31 060999031 tox120,57M -G 4,4/19/99 32 060999032 to x 1 2 0 ,5 8 M -6 4 ,4/19/99 33 060999033 tox120,59M -G 4,4/19/99 34 060999034 tox120, rbl02229-250ppb-cc4 35 060999035 methanol blank 36 060999036 tox120,61M -G 4,4/19/99 37 060999037 tox120,62M -G 4,4/19/99 38 060999038 tox120,63M -G 4,4/19/99 39 060999039 tox120, rbl02229-250ppb-cc5 40 060999040 methanol blank 41 060999041 tox120,169F-G 5,2/22/99 42 060999042 to x120.170F-G 5,2/22/99 43 060999043 tox120,171F-G 5,2/22/99 44 060999044 tox120, rbl02229-250ppb-cc6 45 060999045 methanol blank 46 060999046 . tox120,173F-G 5,2/22/99 47 060999047 to x120.174F-G 5,2/22/99 48 060999048 to x120,175F-G 5,2/22/99 49 060999049 tox120, rbl02229-250ppb-cc7 50 060999050 methanol blank 51 060999051 tox120,73M -G 5, d 25 0,4/19/99 52 060999052 tox120,74M -G 5, d 25 0,4/19/99 53 060999053 tox120,75M -G 5, d 25 0,4/19/99 54 060999054 tox120, rbl02229-250ppb-cc8 55 060999055 methanol blank 56 060999056 tox120, rbl02229-H2Oblk-2 57 060999057 tox120, rbl02229-Uverblk-2 58 060999058 tox120, rbl02229-5ppb-2 59 060999059 tox120, rbl02229-10ppb-2 60 060999060 tox120, rbl02229-25ppb-2 BACK TO MAIN <7 Page 1 MS File PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS -PFHS PFHS -PFHS -PFHS PFHS -PFHS -PFHS -PFHS -PFHS -PFHS ` -PFHS -P F H S -PFHS -PFHS -PFHS -PFHS -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S In le t File p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl p fb s l p fb s l pfbsl p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl pfbsl pfbsl p fb s l p fb s l pfbsl pfbsl p fb s l p fb s l pfbsl pfbsl pfbsl pfbsl r pfbsl p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl p fb s l pfbsl pfbsl pfbsl pfbsl pfbsl pfbsl p fb s l pfbsl pfbsl pfbsl pfbsl p fb s l pfbsl p fb s l pfbsl pfbsl B ottle 91 91 1 2 3 4 5 6 7 8 9 10 11 92 12 13 14 15 81 92 16 17 18 82 93 19 20 21 83 93 22 23 24 84 94 25 26 27 85 94 28 29 30 86 95 31 32 33 87 95 34 35 36 88 96 37 38 39 40 41 > W ml IcM Z fM - u ( / M Masslynx - Sampie U st ' Sample Ust: C:\MASSLYNX\am elia-rat3.PRO\Sam pleDB\060999a.SPL Printed:W edJun 0 9 1 5 :0 1 :1 9 1 9 9 9 File Nam e 61 060999061 62 060999062 63 060999063 64 060999064 65 060999065 6 6 060999066 F lIe T ex t tox120, rb!02229-50ppb-2 tox120, rbt02229-100ppb-2 ta x i20, rbl02229-250ppb-2 tox120, rb!02229-500ppb-2 1 0 x120, rbl02229*750ppb-2 tox120, rb!02229-1000ppb-2 v / y BACK TO MAIN /n&z _ . Page 2 MS File PFHS PFHS PFHS PFHS PFHS PFHS In le t File pfbsl pfbsl pfbsl pfbsl pfbsl pfbsl B o ttie 42 43 44 45 46 47 tmkhMM. Tuning Method Report sM . - Method: C :\MASSLYNX\AMELIA-RATS. PRO\ACQUDB\PFOS Printed: Wed Jun 09 15:01:35 1999 BACK TO MAIN Page 1 MS SOURCE ( ESP- ) Capillary Cone Extractor RF Lens ' Source Block Temp. Desolvation Temp. Set Rdbk 3.00 15 5 0.2 150 250 2.98 -60 -5 150 251 Pressures Analyser Vacuum Gas Cell ' Rdbk 1.4e-4 4.9e-3 MSI LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier , .*X MS2 LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier Set Rdbk 15.0 15.0 2.0 0.0 5 650 i? 6 Set Rdbk 15.0 15.0 30 2.0 0.0 40 0 650 47 37 44 h m U (k b b W ii S A tj ( e / M j t f Scanning Method Report Method: C:\MRSSLTNX\AMZI.ZA-R1XS.pnoVCOUDB\ -ma last Modifind: wad Jun 0* 14:17:17 IMS Frintad: Wad Jun 0* 15:01:0 1599 advent Dalay { mina ) : 0.00 yenetion : 1 M M of 2 Maaa Paira (lap- ) Intar Channel Dalay ( Sacs ) * 0.03 Span-( Daltons ) : 0.00 Start TIsm (Mina ) > 0.00 End Tlaw (Mina ) : 13.50 Repeats : 1 Channel Parent Daughter Duali (Sacs) Coll Enargy (VI Co&a ( V 1 1 299.00 80.00 0.40 2 299.00 99.00 0.40 45 45 60 60 nwotiosa t 2 M M o f 2 Mua Paira ( ESP- ) Inter Channel Dalay ( Secs ) : 0.03 Span { Daltons ) i . 0.00 Start Tina (Mins ) : 0.00 * End Tiae (Mins ) : 13.50 Rapaste : 1 Channel Parent Daughter Duali (Sacs) Coll Energy (V) Orna ( V ) 1 399.00 00.00 0.40 * 45 . 2 399.00 99.00 0.40 45 60 <0 (s/ BACK TO MAIN Paga 1 r / \ u ; UAIAW.V1JLW auiviiVIAKY FORM BACK TO MAIN Oste: (,/l/3 ICO Am U 062499: quwtiatad ind prlnfead by boj 9/10/99 A A A A A A a M WU> m U A U l U U l U I A A A * * A * A A A U U U U U U U U U W M N M M M N M M M M H H H M H H H H H H A S -U M H O U B 'J A A A U M W O U A > )A A * U K lH O W > lA U * U N H O a > J A W * U M H O W tt^ A W * U N H O U > J llU I * U M M | OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOS rs r <0 . ihfhlhfihlhi&k h hf*r ** *** * *3*3 ** * * **** *** * *** * ** * * *3**m **m1g ooogwgo Mw : o o o o o o o o t 4 4 *4 4 0044 4 4 4 4 . 1 *4 4 <4 4 <4 4 4 4 4 4 . 4 4 4 4 4 4 4 4 4 55330 0 0 * 0 1 0 0 o OUOWOOH IIUA'JHO N W 0 w 0 *0 (A *0 W *0 4 Ut OO O MA 4 0 AHN 000 AUA o o o* o o m 0000 o o 00000 00040 0AW 04 000000000 A AA 40 0O 0 0000H H O A 0 w BSa u h h UAUAAAO HWUHWVA M AWAUH M UU UUA U U A U A NslA *H t0H AUHOMWW gNFWI* M O o o o g U HHHH AAAUOUA 1 - o o * N, M O M g .: '<A> W A` E 0i- o u M (4 uM S M 10 mm A M 0I 4H O W*HW 0 0)0 N A 0O 8 *0 14 UOHA lOM0| ooo ESSE UM M A4UH N 00N 0M AH gg*g <0 * * S UMH 0 0 ( 0 0 ) -4 M H M 4 N M 4fc 0 M A 4 H M 0 A A A 0 4 U M 0 4 O M H MW0OWUHMU u o01 U 0 0 0 M 00 A *0 0 4 0 0 g Sii E WNNMN AAMA0 H U N 0U issbs iiiSi M U0M U ^4 O *4 O 0 0 0 A* M 4000040U iibsbbbsk giiiiiiiS 8M u Aw sM M i li l M < H HM 1 U OUM N000 0 0 ! DO > o I* H O > Quantify Ceavound m m m m r y Eaport toxl20, and p(ha ' ' Saagila Uati C:\IASnnec\aMlla-iata.MO\daaplan\OCOtMa Last aodlflad: Thu Jun 10 07:03:0 19*9 Hathod: C :\HassLTKX\anaUa-rata.T0\MathOS\0*Ot9la Laat odlfiod: Thu JUn 10 04:47:27 1 M Job Coda: Frlntad: Thu Jun 10 07:13:33 1** Ccapeund 2: I M 1 Mann anplallat Tact . 1 060999001 athanol blank 2 060999002 athnnol blank 3 060999003 toxl20, rbl02229>B2Cblk-l 4 060999004 toxl20, rbl02229-Llvarblk-l . 5 060999005 toxl20, rbl02229*5ppb"l 6 060999006 t0x120, rb!02229>10ppb-l 7 060999007 toxl20, rbl02229>25ppb-l $ 060999008 toxl20, rbl02229*50ppb-l 9 060999009 toxl20, rbl02229~100ppb~l 10 060999010 texl20, rbl02229-250ppb>l 11 060999011 toxl20, rbl02229>SOOppb-l 13 060999012 toxl20, rbl02229-75Oppb-l . 13 060999013 COX120, rbl02229-1000ppb-l 14 060999014 . nathanol blank 15 060999015 toxl20, 10tr-Cl-250ppb-m, 2/22/9 I 10 060999016 17 060999017 11 060999019 19 060999019 toxl20, 106F-d-250ppb-ad, 2/22/99 texl20, 3K-l-250ppb-aa, 4/19/99 toxl20, 3K-<31-2S0ppb-uad, 4/19/99 toxl20. rbl02229-25Oppb-ccl 20 060999020 nathanol blank , 21 060999021 tOX120, 153r-64. 2/22/99 " 22 060999022 toxl20, 154r-C4, 2/22/99 I 23 060999023 24 060999024 25 060999025 26 060999026 eoxi20, issr-c4, 2/22/99 tOX120, rbl02229-25Qppb-cc2 nathanol blank toxl20, lS7r-G4, 2/22/99 27 060999027 texl20, 1SSIM4, 2/22/99 28 060999028 toxl20, 159C-G4, 2/22/99 ' 29 060999029 toxl20, rbl02229-250ppb-cc3 30 060999030 nathanol blank 1 31 060999031 * toxl20, 57M-G4, 4/19/99 32 060999032 toxlZO, 58M-G4, 4/19/99 33 060999033 tOX120, 59M-G4, 4/19/99 34 060999034 * toxl20, rbl02229-250ppb-cc4 35 060999035 nathanol blank 36 060999036 toxl20, (1M-G4. 4/19/99 37 060999037 toxl20, 62M-G4, 4/19/99 38 060999038 toxlZO, (3M-G4, 4/19/99 39 060999039 toxl20, rbl02229-250ppb-ee5 40 060999040 nathanol blank 41 060999041 tox!20, 149F-GS, 2/22/99 1 42 060999042 toxl20, 170r-Ci, 2/22/99 I1I 43 060999043 44 060999044 tox!20, 17lr-GS, 2/22/99 toxl20, rbl02229-250ppb-ce( 1 45 060999045 . nathanol blank ' 46 060999046 toxl20, 173F-G5, 2/22/99 47 060999047 toxl20, 174F-6S, 2/22/99 48 060999048 toxl20, 17Sr-fiS, 2/22/99 ' 49 060999049 toxl20, rbl02229-2S0ppb-cc7 1 50 060999050 51 060999051 52 060999052 nathanol blank . toxl20, 73M-GS, d250, 4/19/99 toxl20, 74M-GS, d250, 4/19/99 53 060999053 toxl20, 7SM-GS, d2S0, 4/19/99 54 060999054 toxl20, rbl02229-250ppb-ccl I 55 060999055 56 060999056 57 060999057 58 060999058 nathanol blank toX12Q, rb!02229-B20blk-2 toxl20, rbl02229-U*orblk-2 toxlZO, rbl02229-5ppb-2 . 59 060999059 toxl20, rbl02229-10ppb-2 (0 0(099(0(0 toxl20, rbl02229-29ppb-2 61 060999061 toxl20, rbi02229-50ppb-2 62 060999062 toxl20, rbl02229-100ppb-2 1 3 060999063 4 060999064 toxl20, rbl02225-250ppb-2 toxl20, rbl02229-S00ppb-2 65 060999065 toxl20, rbl02229-750ppb*2 66 060999066 tox!20, rbl02229-1000ppb-2 I BACK TO MAIN hgi 2 *yp Analyta ltd Cono Analyta Analyta Analyta Standard 5.99 Standard 12.00 Standard 29.90 Standard 59.90 Standard 120.00 Standard 299.00 Standard 599.00 Standard 898.00 Standard 1198.00 Analyta Analyta Analyta Analyta .Analyta QC . 299.b0 Analyta . Analyta Analyta Analyta QC 299.00 Analyta Analyta Analyta Analyta QC . 299.00 Analyta Analyta Analyta Analyta QC 299.00 Analyta Analyta Analyta Analyta QC 299.00 Analyta Analyta Analyta Analyta QC 299.00 Analyta Analyta Analyta Analyta QC 299.00 Analyta Analyta Analyta Analyta QC 299.00 Analyta Analyta Analyta Standard 5.99 Standard Standard 12.00 29.90 Standard 59.90 Standard 120.00 Standard 299.00 Standard 599.00 Standard Standard 198.00 1198.00 XT 9.010 9.038 9.165 9.151 9.092 9.047 9.045 9.024 9.038 9.043 9.055 9.026 9.038 9.043 9.049 9.051 9.021 9.057 9.032 9.031 9.041 9.058 9.031 9.052 9.068 9.074 9.050 9.068 9.061 9.042 9.065 9.060 9.069 9.055 9.072 9.053 9.084 9.099 9.060 9.101 9.082 9.069 9.064 9.096 A n a Cane. flag. IDov 10 52 23 32 (1 122 313 57( 915 11(9 10.99 bb 55.63 bbX 24.34 bbX 34.(3 bb (5.12 bb 130.54 bb 33t.5( bb (11.51 Ml 9(2.(1 bb 1277.01 bb 245 2(3.40 bb 271 291.19 fab 329 353.29 bb 3(4 391.19 bb 299 320.77 bb ". 'A 829 103 15 9 9 13 3 9 7 7 2(4 203.24 bb ` -5 340 3(4.13 bb 22 *2 '7.11 bb 11( 124.24 bb 1(2 173.(7 Ml 2(1 301.(2 bb 1 37 40.10 bb (2 (4.04 MM (7 71.93 bb 2(9 299.43 bb -3 215 305.44 MM 230 244.44 bb 251 2(9.10 bb 250 2(8.60 bb. 10 41 26 49 24( 1034 002 (4 223 43.75 MM 28.12 bb 51.37 bb 2(3.(( bb ` 1109.17 bb (1.06 bb . 949.1 bb s 239.59 bb -12 v* -20 . \. . 30 S( 112 < 241 X 479 . 741 1079 32.40 bb 59.14 bb 120.41 bb 210.16 bb 514.12 bb 795.99 bb 1157.( bb 8 0 0 -6 -14 -11 -3 - Inaila 0(2494: quantlatad and printed by bai (/10/t I fluaatliy Calibratiod Waport tarilo, and pflia ' Calibratioo: C:\Mmmnt\MiaHa-aata.7llolVcaim i\00>Ha tHt BOdiriadl ItnJ don 10 07sllll7 1M( Trlntad! Thu Jan 10 07iU>23 19*1 BACK TO MAIN Aaalla 0<349li uantlatad and prlatad by fcoj f/10/lt