Document r6NZZ4b5Y46bXm3jXbnbkw1w7
R&S 109570
310-MEDICAL RESEARCH
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TRANSL. MID. NO. 11,459
NTC.` NO.1
GIGIENA I SAMTAHIIA.
V. (7)111-112(1978)
Mutagenic activity o vinyl
chloride.
AUTHOR:
Nuramov, M
M
TRANSLATOR: 1TB
DATE:
Hecemberi 1978
REQUESTED B7:
D. WroblewsU, 1800
TRANSLATION NO.: 78-10-
33
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1 R&s 109572
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UDC 612.6.052.014.46:547.322.3
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MUTAGENIC ACTIVITY OF VINYL CHLORIDE
Candidate of Medical Sciences M.M. Muramov and C.I. Gus'kova, Uzbek Scientific Research Institute of Public Health, Hygiene and Occupational Diseases; Rostov
Medical Institute We conducted an experimental study by means of around-the-clock, chronic (3*j months) inhalation priming of non-pedigree, male, white rats to obtain hygienic substantiation of the permissible level (DU) of emission of vinyl chloride from polymer construction materials. Studies were conducted at two temperature regimes (22C and 35C) on seven groups of animals which were subjected to the effect of different concentrations of vinyl chloride (10, 0.4,
3 0.15 and 0.07 mg/m ).
We used several integrated and specific tests in the course of the experiment in order to reveal the overall toxic resorptive effect of vinyl chloride. In parallel with this, after completion of the priming period, we studied three animals from each group for the purpose of establishing the mut genic effect of this or that concentration of vinyl chloride. We studied the cytogenetic effect of vinyl chloride with the use of the anaphase method of calculating chromosomal reconstructions in the white rat bone marrow cells. Chromosome preparations were prepared by the conventional method which includes the following stages: decapitation of the animals, isolation of the femurs, removal from them of bone marrow by a fixator (a mixture of alcohol and glacial acetic acid in a 3:1 ratio), fixation of bone marrow in the cold (4C) and creation of a cellular suspension from it, preparation of specimens (by enlarg ing on clean microscope slides), staining them (azure-eosin by the Romanovskiy method) and analysis of the chromosomes with the use of a'Siolam-70" microscope with 10 x 40 enlargement. We analyzed 800-1000 anaphases from each group of animals, considerating, during analysis, fragments, chromosomal and chromatid bridges and agglutination. Results of the study are shown in the table.
1
Results of the Cytogenetic Study of White Rat Bone Marrow, Subjected to Chronic. Around-the-Clock Inhalation Effect of Vinyl Chloride
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Ac seen front tho table, the percent of obberant cells in control animals, "ndev different temperature regimes, was practically uniform (4.0 + 0.62 and 3.8 + 0,60; R>0,0.*>), Analysis of bone marrow cells of the animals subjected to around-the-clock, chronic, inhalation effect of vinyl chloride
indicates that, under conditions of normal temperature, only the most minimal
of the three studied concentrations of gas (0.15 mg/m ) did not cause a reliable
increase in the percent of anaphases with reconstructions. During this, the 3
density of fragments in animals of the 3rd group (0.15 mg/m ) was not statis
tically reliably different from that of the control animals at the same time
as the percent of chromosomal bridges exceeded the spontaneous level more tl
1^ times and the density of chromatid bridges was drastically reduced in comparison with the control level. Concentrations of 10 mg/m3 and 0.4 mg/m3
caused, in the experimental animals, a reliable increase in the level of ana-
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cerns the relationship of types of reconstructions in animals of these two groups,
we must note that, in all forms of disturbances, both concentrations caused re
liable absolute and relative changes. The study of the effect of vinyl chloride 3
in combination with high air temperature showed that a concentration of 0.07 mg/m.
does not cause, in the animals, a pronounced increase in the level of aberrant
cells, although changes of density of types of reconstructions are reliable. 3
A concentration of 0.15 mg/m , at high temperature, led to an increase in the
number of aberrant cells by more than 1^ times, causing, during this, changes
in the structure of the reconstructions. These data justify the conclusion that
the concentration of 0.15 mg/m of vinyl chloride, at 22 C, does not, by itself,
cause an increase of aberrant cells, however, this same concentration in combination witn high temperature (35C) is obviously mutagenic, since its effect on
experimental rats leads to an increase of the number of animals with disturb
s.
on the one hand, and to changes in the relationship of types of chromosomal
injuries, on the other. In addition to this, in animals of this group, chemical
and temperature factors evidently affect the reparation systems of cells which is indicated by the fact that the number of fragments in experimental animals
increased while the level of chromosomal bridges decreased reliably.
In resume, we may conclude that a concentration of vinyl chloride of 3 0.07 mg/m does not cause an increase in the level of aberrant cells in bone
marrow tissue of the experimental animals while the other concentrations
(10, 0.4 and 0.15 mg/m ) are genetically dangerous under these same temperature
conditions.
Submitted 11 August 1977
R&S 109574
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112
