Document qmz7wpzrnJJq0O5z5YwRm1OdR

AR 56 - OE!7 TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not given. The test sample is FC-206CE (5/23/91). It's purity was not sufficiently characterized, though current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole.. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:_________________________________________________________ Method: OECD 201 and EPA-600/9-78-18 Type: Acute Static GLP: No Year study performed: 1991 Species: Selenastrum capricomutum Source: Originally obtained from the Culture Collection at the University of Texas at Austin, maintained in culture medium at EnviroSystems, Hampton, NH. Element basis: Algal cell counts (cells/ml). Exposure period: 96 hours Statistical Methods: Performed using standard statistical techniques (Stephan, 1983). EC50 values were calculated using the binomial or moving average method. The NOEC was calculated using Dunnett's test. Analytical monitoring: pH and temperature. Test Conditions: Algal Nutrient Medium: Nutrient medium per U.S. EPA, 1978 prepared in deionized water. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was ~8.0. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared in algal medium. Aliquots were added directly to algal medium in test vessels to create test solutions. 001802 Exposure vessels: 250 mL Erlenmeyers containing 100 mL test solution and capped with inverted glass beakers. Agitation: Continuous rotary shaker, rate of rotation not given. Number of replicates: 3 Initial cell loading: 1.0 x 104 cells/mL Number of concentrations: five plus a blank control Lighting: Continuous lighting at 50 uEs'1m'2 using cool-white fluorescent lights. Water Chemistry: pH range (0-96 hours): 8.2 - 9.9 (control exposure) 7.9 - 8.3 (1000 mg/L exposure) Test temperature range (0-96 hours): 23.0 - 23.4 C (incubator) RESULTS Nominal concentrations: Bk control, 150, 250,400, 600, and 1000 mg/L Algal Growth Response at 96 Hours Nominal Cone., Avg. Cell Count Avg. Specific % Change'1' mg/L (cells/mL) Growth Rate Bk. Control 479,000 0.040 0 150 718,000 0.045 -12 250 415,000 0.039 2 400 242,000 0.033 17 600 0 0.000 100 1000 0 0.000 100 (1) Average growth rate in control compared to test concentration growth rate. Element values: 96-hour ErCso: 96-hour NOEC: 456 (400-600) mg/L 400 mg/L Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not determined. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. 001803 CONCLUSIONS FC-206CE exhibits a 96-hour ErCso of 456 mg/L with a 95% Confidence Interval of 400 to 600 mg/L. The 96-hour No Observed Effect Concentration (NOEC) was 400 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ Test was conducted by EnviroSystems Division of Resource Analysts Incorporated, Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J1884-2, 1991. OTHER___________________________________________________________ Last changed: 6/26/00 001804  v.- . . . '> Robert L. Boeri Tieothy J. Ward Study Completed ^ly, 1991 : BEST COPY AVAILABLE z. stmuiT Tht acute toxicity of FC-206CS to tht alga, Stltautrua ctpricorautua, ia described in tbia final report. The test was conducted for 3H Conpany for 96 hours during July 8 to July 12, 1991, at the EnviroSysteas Division of Kesource Analysts, Inc. in Banpton, Bew Banpshire. It was conducted by Jeanne Magazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Tinothy Bard. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a naan tenperature of 23.2aC. The dilution water was synthetic aedia prepared with sterile deionized water. Flasks were incubated on a rotary shaker. Light was adjusted to SO uEin/sec/a* with a photoperiod of 24 hours light and 0 hours dark. Roainal concentrations of rc-206CE were: 0 ng/L (control), ISO ag/L, 2S0 ag/L, 400 ag/L, 600 ag/L, and 1,000 ag/L. Hoainal concentrations were used for all calculations. Algae used in the test was froa an in-house culture that was aaintained under test conditions. Exposure of algae to the test substance resulted in a 72 hour EC50 of 418 ag/L FC-206CE and a no observed effect concentration (BOEC) of ISO ag/L. The 96 hour EC50 and M0EC are 456 ag/L and 400 sg/L, respectively. / \. W,'. BEST COPY AVAILABLE n . t u u or corrxrri SECTION: 1. Susaary II. Tabla ol Contenta III. Index of Tablee IV. Introduction V. Methods and Materials VI. lesults VII. Keferences Appendix k. Viter Quality Data froa Toxicity Test mw fe ; - liK :i.'** ' i&'il'-.fe WS&-T?. , ^S& ft U . .V".;?, H, & <S As.j rt * % f c ' feT j~r *j .ir 001S07 BEST COPY AVAILABLE IV. INTRODUCTION This study * sponsored by 3M Company, St. Paul, Minnesota. The objective of ths study was to determine tha acuta toxicity of FC-206CE to a freshwater alga. Tha report contains sections that describe tha nethods and materials employed in tha study, and tha results of tha investigation. Tha report also contains an appendix that presents tha water quality data collected during tha test. METHODS M S MATERIALS TEST SUBSTANCE: FC-206CE (Envirosystems Sanpla Eunbar 2936A) was delivered to SnviroSysteas on May 23, 1991. It was contained in a 40 o:. plastic bottle that was labelled with the following intonation: "J1884-2, FC-206CE". FC-206CE (a yellow liquid) was supplied by 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample (approximately 1.0 gram) will be retained at EnviroSystems for a minimum of 10 years. DILUTION WATER: Water used for acclimation of test organisms and for all toxicity testing was sterile enriched media (U.S. EPA, 1978) with a pH of approximately 8.0. Results of chemical analysis of a representative sample of deionized water used to prepare the media are presented in Table 1. TEST ORGMISM: Algae used for the test was from a culture originally procured from the Culture Collection of Algae at the University of Texas at Austin and delivered to Envirosystems on May 14, 1991. The identity of the culture was verified using an appropriate taxonomic key. . The culture was* transferred- to sterile enriched media identical to media used for this , -v- test and maintained at test conditions. TOXICITY TESTING: ssr-Vs The.^definitive ^toxicity test > was -performed during July 8 to 12, 1991. Xtjris.Jbasedon-fprocedures / of -the OECD ( 1 9 8 4 ) The test was conducted/ at * a target' temperature of 22 i 2C with five concentrations of, p&Vtest substance H and a dilution; watertcontrol'.V A 1,000 mg/b stock solution >as .pre.par.ed/in dilution water. ..The stock solution was added dirctly t dilution .water contained$ i% th# test >vessels without the : use*.of a solvent. / .Nominal'^concentrations of Ithe ^test material :'were .0^mg/b (control), 150 mg/b,/250 mg/b; 400 mg/b, 600 sg/L, and 1,000 mg/b. .r: .V -^ 7V' 5 of `ig-Sfew* - T.- T;' ''-i "RMouce AndySs.Inc.SubSctay ef H lP O | | BEST COPY AVAILABLE Tahiti. Chemical characterization of a representative staple of deionized water used to prepare teat nedia for toxicity test Parameter Unit of Keasurenent Reporting Limit Reasured Value Organochlorine pesticides ug/L 2 HD Organopbosphorus pesticides ug/L 0.5- NO Polychlorinated ug/L 0.5 ND biphenyls Rote: IB not detected at or above the reporting limit. 'fiiUi b e s t c o p y Av a il a b l e '-J Algae vaa randomly and fequally distributed among three replicate* ot each tcaataant at the rate ot 10,000 etlla/nl. The test was performed in 250 ml glass lrlenmeyer flasks that contained 100 al of taat aolntion. The flask* vara capped with inverted glaaa beaker*. Teat vessels were randomly arranged on a rotary ahaker in an incubator daring the 96 hour taat (a random number* table mam used to aalect the ;location of each vaaael). A 24 hour light and 0 hour dark pbotoperiod .waa maintained with cool-white fluoreacent light* that provided a light intenaity of 50 uXa"lmr*. The number of algal cella/ml in each taat vaaael vaa determined viaually every 24 hour* by mean* of direct microacopic count* with a hemacytometer. The pH (Beckman model pHX 51 meter) vaa determined in each taat flaak at the beginning and and of the teat, and temperature (A3TH mercury thermometer) waa' meaaured and recorded daily in the Incubator. '* iaV-; 1 STATISTICAL MITH0DS: Xeaulta of the toxicity;teat were interpreted by atandard .atatiatieal technique* (Stephan/ 1983). The binomial or moving average method vaa uaed by the author to calculate EC50 value* uaing nominal concentration* of teat substance., The NOKC was calculated using Dunnett's test, which includes:/; a parametric one-way analysis of variance (AROVA). The average apecific growth rate was calculated as .the natural log of the number^-of cells per ml at 24, 48, 72, or 96 hours minus the natural log ot|the'number of cells per ml at 0 hours divided ;by the exposure V period.^ The percent change from control is calculated by subtracting thejfaampl* average specific growth rate from . . the control average / specific^irowth rate, dividing that value bythe average, specific growth rate^in^the control and multiplying that value., - W f by 100. The percent change from control was used to compute K50jand^ '; VOBCvalues ,3?'*V ,-r-,vterf**6 C*'/*J3p*$j & ty m 001811 Biological and water quality data generated by the acute ' toxicity teat are preaented in Table 2 and Appendix A, respectively, and the percent of control and average epecific growth rate intonation ia preaented in Table 3. -The 24, 48, 72, and 96 hoar EC50s for algae expoaed to rC-206CE are preaented in Table 4. The 72 hour BC50 is 418 ag/L, and the 72 hour KOEC is 150 ag/L TC-206CK. The 96 hour IC50 is 456 ag/L and the 96 hour NOEC is 400 ag/L. BEST COPY AVAILABLE V rc'-f' Table 2. Growth data fro toxicity teat Moainal Concantration - (g/L) rep. 0.0 (control) Ruaber of Cell per Milliliter x 10* (hovr) 0 2 48 72 96 150 V 250 400 36 38 26 33 25 20 15 20 25 10 30 ; 22 104 130 105 113 90 65 85 80 45 70 55 57 *&'{2 *3 - ro'7 v . & * & * w ^ K * * * * r : io ; -V , \ ,, **** "'> * I vr *' . - - ;" -, . l.ooo . - , i io . v.i;. , ^ * % - v vA v-r-'.-v ' V f * v a -t: - ~ . bis r .-,v . . *>-*. i i o ^ v v is.,;/V**'* * <*. grt.' >W liil 0 ro.:l f-^YO 'Mblrte :. .'>7-:*.''/^a;.' :;?:Va, Table 3. Percent change from control and average pacific growth rate iron toxicity teat Nominal Concentration of Test Substance (eg/li) Average specific growth rate 24hr 48hr 72hr 96hr Percent change froa control 24br 48hr 72hr 96hr 0.0 (control) 150 250 400 600 1,000 0.020 0.052 0.041 0.040 0.029 0.025 0.034 0.045 0.017 0.015 0.029 0.039 0.022 0.017 0.024 0.033 0.035 0.000 0.000 0.000 0.017 -0.007 0.000 0.000 0000 -45 52 17 -12 15 71 29 2 -10 67 41 17 -75 100 100 100 15 86 100 100 b e s t c o p y a v a il a b l e BEST COPY AVAILABLE ** Table 4. Median effective concentrations (XC50s) tros toxicity test Exposure period EC50 95 percent confidence Units Calculation nethod 24 hours >1,000 sg/Ii 48 hours < 150 ng/L 72 hours 418 ng/L 96 hours 456 ng/L -- 250 - 600 ng/L 400 - 600 ng/L -- -- Binonial/nonlinear Interpolation Binonial/llonlinear Interpolation Yii. ucrmxcxs b e s t c o p y a v a il a b OBCD. 1984. Guidelines for Testing of Chemicals. Section 2: Effects on Biotic Systens. Method 201, Algs, Growth Inhibition Test. Adopted June 4, 1984. Stephan, C.B. 1983. Coaputar progran for calculation of 1X50 values. Personal connunication. O.S. EPA. 1978. The Stlaaastrue eapricoroutua Prints Algal Assay Bottle Test. EPA-600/9-78-018. July, 1978. Tabi* .l. Tenpersture measured during toxicity tast Day of Exposure 0 1 2 3 4 Teaperature of Incubator,C 23.2 23.0 23.0 23. 23.2 BEST COPY AVAILABLE