Document qkM9jJ0z8532Lj75mrrRwJyej
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k u t o Toxicity O f U2803-2 to tho Daphnid, DapJJnfa n r g m
Data Requiromontr Guidolino Numbor U.S. EPA-TSCA, Guideline 791.1300
Authors Timothy J. Ward Peter L . K W A l s k i Robert L. soeri
Study Cooploted November 16, 1995
Report Amonded narch 25, 1996
Sponsor 3n company EnVirOnuientAl Laboratory 935 Bush Avenue Building 2-33-09 St. Paul, ninnesota 55144
Tosting Facility T.R. Wilbury Laboratories, Inc.
4 0 DoAkS Lane Uarblehead, UASsAChUsetts 01945
T.R. Wilbury'study Number 889-TB
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!
This mtudy was conductad according t o LPA Good Laboratory P r a c t i c a
RaguhtiOnm (40 C?R 7 9 2 ) , with tho f o l l w i n g oxcaption. Tho m t a b i l i t y of
t h e tamt mubmtanca wam Um-d
but not varifiad a t T.R. n i l b u r y
Laboratoriem. cmviations from t h a mtudy protocol and protocol amendments
are prasantad in Section XI1 of t h i m raport.
Tha f i n a l r a p o r t was amanded on W c h 25, 1996 t o include t h a d a t e of t h a amandnmnt on pagam 1 and 3, t o allow f o r signaturam t o t h a aman&d
report on pages 2, 3, and 18, t o modify t h e 24 hour EC50 on pagem 6 and 16, t o modify t h a graph on page 15, rad to include t h e amendment summary
on t h i s page.
StGdy Director
T.R. W
.. .
. ..
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Amended 3/25/96
Subatitted by:
T . R . Wilbury Laboratories, Inc. 40 Doaks Lane Marblehead, Xlrrsachuretts
Certification:
T.R. Wilbury Laboratories, fnc. study number 889-~11, Acute Toxicity of N2803-2 to the tmphnid, Daphnia magna, was audited by the Quality Assurance unit for compliance with the protocol, standard operating procedures, and applicable Good Laboratory Practices (g.8. EPA, 1993). Quality assurance audits were performed and the findings reported to T.R. Wilbury Laboratories management and the study director on the following dates:
Aspect Audited
Protocol: In-life: Draft Report: Final Report: Amended Report:
Audit Date
8/ 3/95 9/ 5/95 9/25/95 11/16/95 3/25/96
Reported to study
-- Director
8/ 4/95 9/ 5/95 9/25/95 11/16/95 3/25/96
Reported to
Management
8/ 4/95 11/16/95 11/16/95 11/16/95 3/25/96
Quality Asaurance officer
T . R . wilbury study tiumber 889-~n
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I. oood Laboratory Practice compliance statement
2
11. Quality Msurance statement
3
111. Table of Content.
4
IV. Index of Tables and Irigurea
5
V.
6
VI. General Information
7
VII. Introduction
8
VIII. nethods and Haterials
e
IX. Results
13
X.
Ref erencea
17
X I . signature Page
18
XII. Protocol Amendments and Deviations
19
Appendix A. Water Quality Data from Toxicity Teat
20
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XV. IlID.1 01 TABLE8 AnD .IOUW
Table 1. Table 2. Table 3. Table 4. Table A.l. Figure 1.
Chamical c h a r a c t e r i z a t i o n of a r e p r e s e n t a t i v a r q l e of dilution water used f o r thm t o x i c i t y t e s t v i t h daphnids, Daphnia magna, and N2803-2
Test s y s t e m summary f o r t h e t o x i c i t y t e s t v i t h N2803-2 and daphnids, Daphnia magna
Survival and sublethal e f f e c t data from t h e t o x i c i t y test with daphnids, Ddphnfa magna, and N2803-2
Median l e t h a l concentrations (LC50s) and median e f f e c t i v e concentrations (ECSOs) from t h e t o x i c i t y test v i t h daphnids, Daphnia magna, and N2803-2
Dissolved oxygen concentration, conductivity, pli, and temperature measured during t h e t o x i c i t p test v i t h daphnids, Daphnia magna, and N2803-2
s u r v i v a l and s u b l e t h a l e f f e c t s of daphnids, DapJmia magna, exposed to 82803-2 for 48 hours
Pm
9
11 14 16 21 15
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Amended 3/25/96
V. IKM4&RX
Tho b c u k toxicity Of N2803-2 to tho daphnid, D l p b n i a magna, is described in this final report. The test was conducted for 3x c a p a n y for 48 hours frcm September 5 to 7, 1995# at T.R. Wilhury Laboratories, Ina. in marblehead, Uh88clChUSOttm. It was conducted by Peter xowalski, ~0.~0 Hagasu, Jacqueline Nevius, Keven Stevens, Peter NwfU8, Rob.rt h r i , and Timothy ward according to the protocol developed for T.R. Wilbury study
Wmb.r 889-T8. U2803-2 was sapplied by the sponsor.
Tho test WAS performed under static conditions with five
concentrations of test substance and h dilution water control at a
temperature of 19.3-20.4.C.
The dilution water Was filtered deionized
water collected at Uhrblehead, Xasrachusetts and adjusted to a hardness of
176 mg/L as CACO,. Aeration was not required to maintain dimsolved oxygen
concentrations above an acceptable level.
Nominal concentrations of N2603-2 were 0 (control), 13, 22, 36, 60, and 100 mg/L. DhphnidS used in the test were lesa than 24 hours 011 at the start of the test. They were produced at T.R. Wilbury Laboratories and acclimated under test conditions for their entire life (cultures that produced test specimens were mhintained at test conditions for more than 7 days). After 48 hours of exposure the control daphnids had an average wet weight (blotted) of 0.26 mg. All daphnidr were in good condition at the beginning of the study.
- Exposure of daphnids to the test substance resulted in a 24 hour
median lethal concentration (Lc50) >lo0 mg/L N2803-2, a 48 hour LCSO of 93 mg/L N2803-2 (951 confidence interval 74 to >IO0 mg/L), a 24 hour median effective concentration ( ~ C 5 0 ; based on immobilization) of 72 mg/L
(958 confidence interval = 63 to 83 mp/L), and a 4H hour EC50 of 62 mg/L
N2803-2 (958 confidence interval = 36 to 100 mg-/Ll..
observed effect concentration (NOEC) is 13 mg/L.
The 48 hour no
T.R. Wilbury study Number 889-TX
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naterial Tested: sponsor :
T e m t Substance S h i p p d From:
Reported Purity:
Study Initiation Date: Experimental S t a r t Date: Experimental completion Date: Study Completion Date: Archive Location for t h e Biological RAW Data and a copy of t h e F i n a l Report:
N2803-2 3x cornpuny tnvironnental Laboratory 935 Bu8h Avenue Building 2-3L-09 8t. Paul., Minnesota 55144
3x company tnvfronmntal Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, Minnesota 55144
1008
August 16, 1995 (protocol signed) September 5, 1995
September 7, 1995
November 16, 1995
T.R. wilhury Laboratories, Inc. Warblehend, m s s a c h u s a t t s
T.R. Wilbury Study N u m b e r 889-TII
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E i
VII. IlTnoWCTIol
j Thin atudy war rponaorod by 3X Company, St Paul, Hfnaosota. Tho
objoctivo of the otudy Wa8 to dotermino tho 24 and 48 hour mdim lotbal
k
concontrationr (LC50a) and rwdian offoctive coacontrationa (KC50a) of tho
toat aub8tmco to tho daphnid, Daphafa m g n n , a fro8hvat.r invortobrato,
undor atatia condition#. Tho tort protocol was baaul on W.S. EPA mothod.
(U.S. KPA, 1993). Tho roport contain# aoctiona that dorcribo tho rwthod.
and matorial8 omplayod in tho atudy, and rmaulta of tho invo8tigation.
The report ala0 contain# an appendix that prosento water quality data
collocted during tho tomt.
ii TEST SUBSTAWCX:
VIII. WETBODS AMD UTERIALS
I
The Sample of N2803-2 (T.R. Wilbuw Laboratories Sample Number 520)
j
was delivered to T.R. Wilbury Laboratories on Auguat 14, 1995 in a
cardboard box at ambient temperature. It was containod i n an approximately 50 ml glass jar. The label attached to the bottle included
i
j the following information: "2803-2". N2803-2 (a clear liquid) was supplied by 3H Company, St Paul,
Minnesota. Prior to use the test substance was stored in the dark at rocm
I
temperature. The test substance was assuaed to have a purity of 100.
active ingredient and to be stable under storage and testing conditions.
Unused test substance will be returned to the sponsor.
DILDTIOU WATER:
Water used for acclimation of test organism and for all toxicity testing was deionized water collected at T.R. wilbury Laboratoriea i n Harblehead, msaachusetts. Water was adjusted to a hardness of 160180 mg/L as CaCO, and atored in 500-gallon polyethylene tanka where it was aerated and continuou8ly passed through a particle filter, ultraviolet sterilizer, and activated carbon. Results of chemical analysis of a representative sample of water are presented in Table 1.
TeST ORGAMISJI:
Juvenile daphnids, Daphnia mgna, employed as teat organisms were
l e s a than 24 hour. old at tho start of the test. They we10 from a single SOUTCO and wero identified uaing an approprialm t a x o n d c key. Test s p c h n a were produced by adult daphnids that were maintained under t e s t condition8 at tho T.R. Wilbury facility for more than 7 day..
T.R. Nilbury Study Wumber 889-TH
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Tablo 1.
chemical ChUACtOriXatiOn of a reprorentativo .amplo of dilution wator urod for tbo toxicity tost with d.phnid8, DapJmfa augna, and 192803-2.
Paramsterl
unit of Moaruromont
D.t.ctioa Limit
UOa8lU.d
value
Metals Aluminum Arsonic Boron cadmium chromium Cobalt
copper Iron Lead Mercury Nickel silver Zinc
0.1 0.01 0.5 0.0002 0.01 0.03 0.005 0.03 0.005 0.0003 0.03 0.02 0.02
ND3 WD
ND
WD
ND ND ND 0.03 ND ND ND ND ND
Nitrate
mq/L as I
0.05
ND
Chloride
mg/L
1
Fluoride
mq/L
0.1
ND
Total Organic
Carbon
mg/L
1
ND
Total Phosphorus
mq/L
0.03
ND
Organochlorine
Pesticides
Pq/L
0.5
ND
Toxaphene
P9/L
2
ND
Organophosphorus
Pesticides
P9/L
0.5
ND
Dimethoate
PW
2.0
ND
TEPP
P9/L
2.0
ND
hnocrotophos
P9/L
2.0
ND
0.5
ND
2.
-1995 and analyzed by Pace, lnc.
quality tOSting. tiD not detected at or abovo the
as part of routine detection limit.
water
T.R. Wllbury study Number 889-TH
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-BACK TO MAIN The original culturo wa8 obtainod on Juno 1, 1994 from Aquatio R08euch Organimu, nuapton, Now nampmhire. Masuromontm made during the ? clay8 before tho #tart of the toat indicatod that tho culture tomporaturo rangod from 19.6 to 20.8.C and tho di6~0lvad oxygon concontration was alwaym at leaat 8.0 mg/L. The culture priducod young prior to day 12 and a mubsunple of adult8 produced at least 3 young/&phnid/day for 7 day. boforo tho 8 t u t of the tost.
During acclimation daphnidm w r o not trOAted for dfseame, thoy were froe of apparont micknoss, injuri.8, and abnonnalitios at tho boginning of the tost and thoro wan no mortality during the 48 hour. preceding the start of the teat. Tho culture was supplied with a yeast/trout chow mix (Lot# DC02) and the fro8hwat.r alga, Sedenastnm! capricornutum, daily during acclimation. Daphnid8 were not fod during tho test. Control daphnids, blotted and weighed at the conclurion of the toxicity test, averaged 0.26 mg and the loading rat0 duringthe definitive toxicity teat was approximatoly 10.4 mg/L.
TOXICITY TESTIIIQ:
A static mcreening teat wa8 conducted from August 23 to 25, 1995 with five concentrations of teat subatmcm and a dilution water control. Nominal concentrations of N2803-2 were 0.10, 1.0, 10, 100, and 1,000 mg/L. After 48 hour8 of exposure there Was 100% survival in the control and at 0.10, 1.0, 10, and 0% SUNiVal at 100 and 1,000 mg/L.
The definitive toxicity test Yam performed fram September 5 to 7, 1995, according to T.R. Wilbury Protocol 8fl9-TE (Acute Toxicity of N2803-2 T o The Daphnid, Daphnia magna). Tho protocol, which was signed by the mtudy director on August 16, 1995, is bused on procedures of the U.S. Environmental Protection Agency (1993). A 8ummary of the test system is presented in Table 2.
The test was conducted at a target temperature of 20 i 1.C with five concentrations of test substance and a dilutfcn water contrcl. N d n a l concentrations of the teat substance were: 0 mg/L (control), 13, 22, 36, 60, and 100 mg/L. A 200 mg/L stock solution was formulated by combining 0.200 g test mubstance and dilution water in a Class A volumetric flask and adjusting the final rtock solution volume to 1,000 m l . Appropriate amounts of thim stock solution vera added to dilution water in test vensols to formulato test media without the use of a solvent.
I
Twenty daphnidm w r e indircrhdnately and equally distributed among two replicatom of each treatment. Tho test was perfom3d in 300 m l glans beakorr that containod 250 ml of test solution (water depth was approximately 9 cm). Tert v08sel8 were randomly arranged during the 48 hour tost (a random number. table waa used to aeloct the location of each vessel) and they were loosely covered.
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species : Ap./Sizo Of TOSt Organimmar Test Duration: Te8t VeBEelm: Tam+ W d i a Volume: D o p a of T e s t Uediar Umber of Replicate Test Veaaels: Orgmiarm, per Replicate: Temperature Range: ninimum Dimsolved oxygen: Photoperiod: Light Intensity: Water Q u a l i t y masurementsr
End Points:
Acceptability criteria:
Daph.la magna
e24 b u r r old
48 hoium
300 le1 g l a s s beakers
250 nil
9 cm
L
10
8.6 mgIL
16 how8 l i g h t and 8 hours dark.
110 fcmtcandles
Disso1.ved oxygen, pa, temperature, and conductivity daily i n each test vemel. Hardness, t o t a l organic carbon, and a l k a l i n i t y once i n t h e dilution water. Residual chlorine daily. Dilution water analysis in summarized in ~ a b L c1.
24 and 4 8 hour LC50 and EC50 (based on immobilization), 48 hour no observed effect level. 110% c o n t r o l m o r t a l i ~ t yand acceptable temperature range.
T.R. W f l b U r y Study umber 889-TH
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A 16 hour light and 8 hour dark photoperiod with a 15 minute transition poriod was automatically ~ ~ i n t d n 0width cool-whfte fluoroscont llpht. that providod a light iatonsity of 110 footcandles. Aeration was not roquirod to maintain disro1p.d oxygen concentrations above acceptable l e a l m and &phnids woro not fsd during thm test.
The numbor of surviving organism and the occurrence of sublethal
effect. (immobilization, loss of equilibrium, loss of reflox,
excitability, discoloration, or change J.n behavior) vere determinod
vimually and recorded initially and after 24 and 48 hours. Dead test
organism were removed d o n first observed. Dissolved oxygen (181 n0d.l
18 motor8 instrument number l), pX (wclrman model pa1 12 meter; instrument
number 124), conductivity (cole P-r
meter, instrument:nrtmber 94), and
temperature (Beckman modo1 pa1 12 motor; instrument number 124, probe pm-
4 ) wore measured and recorded daily in each test c h d r that contained
live a n h l s . The temperature in a beaker of water incubated among the
test verraels was recorded continuously during the test.
mn.rI8TIcBL m O D a ;
Results of the toxicity test were interpreted by standard statistical techniquea. Computer methods (stephan, 19113) were used to calculate LC50s and EC5Om. ldl calculation. were perfornted using nominal concentrations of test rubstance and the number of live or innmbilized daphnids. The no obaerved effect concentration ie the highest concentration of teat substance that did not cause any toxicant-related mortality or sublethal effects.
T.R. nilbury study Number 8 8 9 4 8
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water q u a l i t y data gonoratod by t h o aciito t o x i c i t y t o n t u o p r o n o n t d in Teblo 3 end Appondix A, respectively. Tho moanurod concentration# of dinnolvod oxygon rangod from 8.6 to 9.0 mg/L, tho tuPp.raturo rangod from 19.3 to 10.4.C, conductivity rangod from 670 t o 720 p h o n / c m , and pa rangod from 8.3 t o 8.6. On0 hundred porcent nurv.1v.l occurred i n t h o c o n t r o l expoeure and theno daphnfdn d i d not e x h i b i t any nublethal effoctn.
The 24 and 48 hour 1x50. and EC50a for daphnitln exponod t o N2803-2
- aro presentod in Tablo 4. The 48 hour IC50 f o r daphnfdn oxponed t o N2803-
2 in 93 mg/L (95%confidence i n t e r v a l .1 74 t o >lo0 mg/L) and t h e 4 0 hour Ec50 in 62 mg/L (958 confidence i n t e r v a l 36 to 100 mg/L). The a l o p of t h o 48 hour doso responre curvo (baaed on mortality and i m m b i l i s a t i o n ) in 6.1. Tho 4 8 hour no observed e f f e c t concentration [NOEC) i n 13 mg/L.
T.R. wilbury study Number 889-TB
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Tablo 3.
..
survival and sublothrl effeat data from tho toxiaity tort with daphnids, Dapbnia magna, and YZ803-2.
wdnal Concentration
of UZ803-2 (WL)
Mumbar - Alivo - )IRIIPkr nffmcted
0
24
40
0
24
48
mp. hr
br
br
hr
hr
hr
0 (control)
1
10
10
10
2
10
10
10
13
1
10
10
10
2
10
10
10
22
1
10
10
9
2
10
10
10
36
1
10
10
10
2
10
10
9
60
1
10
10
9
2
10
9
9
100
1
10
8
4
2
10
9
3
0
0
0
0
0 0
0 0
0
6
0
8
~
~~~~
~~
Note: Affected daphnids were immobilized at 24 houra and leas active than control daphnids at 48 hours.
3 immobilized daphnids 4 immobilized daphnids
T.1. Wilbury study Number 889-TH
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Amondad 3/25/96
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L
5na,
z 5-
-
i 13
22
36
60
100
Concentration of N2803-2 (mq/.L I
+Live Dophnids
+-Unaffected 7 Daphdds
Figure 1. Survival and sublethal effect6 of daphnids, Daphnia magna, exposed t o 12803-2 for 4 8 hours.
. _:
T.R. Wilbury study #umber 8 8 9 - m
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Amended 3/25/96
, Tabla 6. n o d i m lethal concentration. (Lc508) and median effective concontration. (ECSO.) tram tba toxicity toit with d~phnld., Daphnia magna, and U2803-2.
txpoaure period
Lcso/Lcso
95 parcent confidonco limit
Calculation rwtbod
LC50
24 hours
>LOO mg/L
-.-
--
48 houra
93 mg/L
74 to >lo0 mg/L
Probit
SC50. 24 hours
40 houra
72 mg/L
62 mg/L
63 to 03 mg/L 36 t o 100 mq/L
Probit
Binomial/ tionlinear Interpolation
T.U. Wilbuq study Number 889-TH
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ASTX. 1986. Standard Practice f o r Conducting Acute ToxfCfty T0.t. with
rimhem, UAcroinvertebrator, and Aaphibians. E-729-80.
Annual
Book of Standards.
Stephan, C.E. 1983. Computer Program l!or the Calcul.atioa of LCSO Values.
U.S. EPA. DUlUth, KN. PermOnal C-niCAtiOn.
U.8. EPA. 1993. Toxic Substance# Contxol A c t Temt midelinom; r i n a l Rules. S e c t i o n 797.1300.
u.8. EPA. 1993. 40 CIR Part 792. Toxic substanceu Control Act (TSU);
Good Laboratory P r a c t i c e standards. Code of Federal R e g u l a t i o n s , T i t l e 40, July 1, 1993.
T.R. wilbuxy study Number 889-TH
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..
Aownded 3/25/96
X I , IIQIIATPRE P M E
study D.irector Aquatic Toxicologist
Biologist
Biblogis t
m L %!!!!en- L
Keven U. Stevens Biologist
SIdqG
.&a / I
Ro rt L., Boeri
3-2S-qb
Coauthor
T.R. Wilbury study Number 8e9-Tn
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The protocol wan mended to npecifiy the n d n a l concentrationn for the definitive tent. No other changes were mado to tho protocol. PROTOCOL DEVIATIONS: No deviationn were made from the protocol.
T.R. wilbury study Number 889-TH
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Page 20 of 22
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Tle At. DSDloiumpspncailrsvuetmdiargeneo`,xmyig4renundeNdcEo`SmMucruSinntgeatthieonC,oxlociosnysuccteinvtitwyi,th Spohp,hatdaerrd
_--Ty
Ditclvedoyun (3/2) Condustivivy Gasor/om
E -o_t--ewaarr)es ee w aOE] e wheETE)
Oleemea E1R K wa Cno we Ga0o aa0 a
1
Loe se we ao a0 me
=
Ie se we oo a0 me
100
Loe se a a a0 me
on. wid scady wsser 099-78
{
rage 2108 22
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Tablo A.1. Continued.
Nominal Concentration
of 12803-2 (WL)
~~
0 (control)
13
22
3G
60
100
0
Rep.
hr
~
~
1
8.5
2
0.5
1
0.5
2
0.6
1
0.6
2
0.6
1
0.6
2
0.6
1
0.6
2
0.6
1
0.6
2
0.6
Pff
20
48
hr
hr
8-51 0.3
8.5
0.4
0.5 0.4
0.5
0.4
0.5
0.4
0.5 0.4
0.5
0.4
0.5
0.4
0.5
0.4
0.5 0.4
0.5 0.4 0.5 0.4
Tomporaturo (.C)
0
24
48
brhrhr
20.2 19.4 19.3 20.2 19.5 19.4
20.2 19.6 19.4 20.2 19.5 19.3
20.3 19.4 19.4 20.3 19.5 19.4
20.3 19.5 19.4 20.3 19.6 19.5
20.4 19.6 19.4 20.4 19.4 19.3
20.4 19.3 19.3 20.4 19.5 19.3
T . R . wilbury study Number 009-TH
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03/29/2001
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
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LAB REQUEST NO. N2803 REQUESTOR NAME: SAB/GOEBEL/PICKETT DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 08/01 /1995 DESC: FC-143 AND RELATED PRODUCTS ECOTOX
WORK
CONTRACT LAB(S): TRWIL EXP COMP DATE: 10/04/1995 DATE COMPLETED: 03/27/1996 PROJECT LEAD: PHONE NO: 3M FAX NO: 651-778-6176
Samples received f r o m S.J. M a r t i n .
Samples 1 - 5 sent t o TRWIL on 8/11/95. 8/22/95.
An a d d i t i o n a l 4L o f sample 5 sent on
Sample 1 = T e t r a b u t y l ammonium hydroxide, 40% i n water. Sample 2 = L-13492 (45% s o l i d s , 27% water, 27% IPA) Sample 3 = FC-26, 100% s o l i d s . Dissolve i n 50:50 water:IPA s o l u t i o n . Sample 4 = FC-143, 100% s o l i d s . Sample 5 = L-13856, 15% s o l i d s . Sample 6 = L-13856, d i l u t e d by a f a c t o r o f 15, t e s t e d as an e f f l u e n t .
Sample #1 (Tetrabutylammonium hydroxide) - a new b o t t l e was ordered from A l d r i c h because TR Wilbury needed more sample. This bias l o t 05922BN, red l a b e l , c a t . # 17,878,O. sab 9/11/95
NOTE: A l l data f o r sample 3 (FC-26) i s reported i n terms of FC-26, and n o t
as a 5 0 / 5 0 mixture w i t h isopropanol.
sab 11/21/95
NOTE: For sample #5, o n l y a r a n g e - f i n d i n g t e s t was run f o r t o x i c i t y t o algae. The requestor canceled t h e request f o r t e s t i n g so no d e f i n i t i v e t e s t was run. The r e s u l t s f o r t h e range f i n d e r are as f o l l o w s :
Test Conc .
mg/l
0.1
1.o
10 100 1000 EC50 (95% C sab 2/16/96
Percent
I.1
Difference
i n Avg. S p e c i f i c Growth Rate 96-hrs Expsure
2
18 4 32 100 160 (100 - 1000) mg/L.
For N2803-6, t h e t e s t i n g was canceled before a d e f i n i t i v e study w i t h f i s h could be completed. Results r e f l e c t t e s t i n g w i t h a s i n g l e concentration o f 13% v / v , which showed no m o r t a l i t y a f t e r 96-hours. sab 3/14/96
1 08/11/95 TB NH40H
L o t 10317KQ
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST
27 MG/L
(23 - 32)
* = CONTRACT LAB DATA
03/29/2001 LAB REQUEST NO. N2803
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
CONTINUED
BACK TO MAIN
2
1 0 8 / 1 1 / 9 5 TB NH40H
L o t 10317KQ
TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0
2
L - 13492
Lot 2
7 2 0 MG/L 3.7 MG/L
(600 - 1000) (1.8 - 7.7)
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR
TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0
62 MG/L 960 MG/L 8.4 MG/L
(36-100) (830->1000) (5.9 - 14)
3
FC- 26
L o t 135
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC5O-ALGAE *ALGAE ASSAY - I C 5 0
360 MG/L 140 MG/L 90 MG/L
(300 - 500) (110 - 180) (63 - 130)
4
FC-143
Lot 427
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR
TRWIL IC5O-ALGAE *ALGAE ASSAY - I C 5 0
5 08/16/95 L-13856
As - is
720 MG/L 740 MG/L
>lo00 MG/L
(600 - 1000) (660 - 830)
(-)
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0
190 MG/L
(130-600)
>lo00 MG/L
(-)
SEE COMMENT ABOVE
6
L - 13856
1:15 d i l u t e d
TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - I C 5 0
1.2 % v/v
(1.0
>13 % V/V
(-1
TESTING CANCELED
- 1.7)
* = CONTRACT LAB DATA
