Document qaE9Zbg6rwryVBvDOgZ3OmGOE

AR226-3031 : Study Title CorrositexTM In Vitro Test with H-21333 Laboratory Project ID Haskell Laboratory Report No. 708-95 Author Carol Finlay Study Completed On October 9, 1995 Performing Laboratory E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P. 0. Box 50 Newark, Delaware 19714 1 Page 1 of 4 Company Sanitized. Does not contain TSCA OB? Substance Tested; Synonyms : GENERAL INFORMATION DuPont HLR 708-95 Physical Form: Composition; Contaminant: Purity: CAS Registry No.: Sponsor: Study Initiated - Completed: In Life Phase Initiated - Completed: DuPont Specialty Chemicals E. I. du Pont de Nemours and Company Wilmington, Delaware 9/22/95 - 10/9/95 9/22/95 - 9/22/95 -2- Company Sanitized. Does not contain TSCA CBS DuPont HLR 708-95 CorrositexTM In Vitro Test with H-21333 SUMMARY H-21333 was evaluated for skin corrosion potential using the In Vitro International CorrositexTM assay. The results of the assay were used to determine the United Nations' corrosive packing group. CorrositexTM is a standardized and quantitative in vitro corrosivity test. The model is based on the time required for the test substance to pass through a biobarrier membrane and produce a change in a chemical detection system. Approximately 0.5 g of H-21333 was applied to each of 4 membrane discs. The test substance passed through the membranes 97-122 minutes after application. Under the conditions of this test, H-21333 is a corrosive substance and is assigned to Packing Group III. Reviewed and Approved for Issue CF/alw Study Director - 3 - Company Sanitized. Does not contain TSCA CBf DuPont HLR 708-95 INTRODUCTION The purpose of this study was to determine skin corrosion potential of H-21333 using the In Vitro International CorrositexTM assay kit. The results of the assay were used to determine the United Nations' Packing Group. MATERIALS AND METHODS A. Protocol The biobarrier membrane discs were prepared on the day the assay was run. For the assay, a membrane disc was placed onto each of the 5 scintillation vials containing the chemical detection system (CDS). Approximately 0.5 g of H-21333 was placed on top of the discs of 4 vials. One vial was similarly treated with a positive control (sodium hydroxide). Each vial was observed for an alteration in the CDS. This alteration may include color changes, flaking, or precipitation. B. Records Retention All raw data and the final report will be stored in the archives of Haskell Laboratory for Toxicology and Industrial Medicine, E. I. du Pont de Nemours and Company, Newark, Delaware or in the DuPont Records Management Center, Wilmington, Delaware. RESULTS AND CONCLUSIONS Breakthrough of the biobarrier occurred as follows: Vial 1 (Positive Control) - 6 minutes Vial 2 (H-21333) - 112 minutes Vial 3 (H-21333) - 122 minutes Vial 4 (H-21333) - 105 minutes Vial 5 (H-21333) - 97 minutes Average time for vials 2, 3, 4, and 5 = 109 minutes Under the conditions of this test, H-21333 is a corrosive substance and is placed in Packing Group III. - 4 - Company Sanitized. Does not contain TSC .