Document pmbQJgO3oJm8nwdoRy7VgoYYw
--
APILOT REPRrOosD:SUTUCDYTWiITHOTHNE NORTHERN BOBWHITS FINALREPORT
WILDLIFE INTERNATIONAL, LTD, PROTECT NUMBER: 454104 SMLABREQUEST NO, 223 FIERA Guideline 714
AUTHORS: SRoaauyopnndGLBt.iegVovacsovn
STUDY INTTATION DATE: Petry 26,2000 STUDYCOMPLETION DATE: Dinter 18,2005
Saitedto ov53uBCumopALivaabemsy
StPaul, Minnesota 55106
Wildlife International, Ltd. E5v9i8inCoMomahmyearancdenD2i1v60e1 Page arias
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ProjctmNumebers454-104
2.
GOOD LABORATORYPRACTICE COMPLIANCESTATEMENT
SPONSOR: 3MCorporation
TITLE:
PFOS: APilotReproductionStudywiththe NortherBobwhite
`WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454-104
STUDY COMPLETION: December 18, 2003
Thestadywasconducted icompliancewithGoodLaboratoryPracticeStandardsaspublished
by theU.S. EnviPrr oteco tionn Agem ncye ,40n CFt RPaa rt1l 60, 17 August 1989; OECDPrinciplesof Good Laboratory Practice (ENV/MC/CHEM (98) 17); and Japan MAFF, 59 NobiSan Notification No. 3850, Agricultural Production Bureau, 10 August 1984,withthefollowingexceptions:
Thestudywasconductedunder lilprotocols.Thenif portionwascondcidunderane uS`ntprudodteyonctouwlmo(bWseielrpdai0ri2af3te-eI0np4tr1eowmtaaotsoilosni(alE,ixLytdgdew.insRttehusdyesanerupcamhrsbaedr Sy45y4n-u1mD0b4ee)rc,saon2rd3t-h0e4a1naalyti0c2a5l.p0o6r9ti)onEswxeorencoRnodsuacrtcehd.
porteRdessepuataseloyf.aalyssconductedbyExygenResearch forstudymumbers023041and 023.06 are
STUDYDIRECTOR:
A, b ole SSeeaonrP.BGalilaoghelArvioangToxiiceolo,gy SPONSOR'S REPRESENTATIVE:
TFL Newsnd .
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Le WLilAdlNifEe IntAeRrnRae tOioNnal, Ltd. nPs roTjectNRumbeI r454-N104 5.
QUALITY ASSURANCESTATEMENT bGyotohde LUTaSbh.oirsaEtodrynyPwraavcsticcieoa(PmErriNontVdcoIifMooCrnu/cAoCgmeHpmnlEciMycea,(9480)nwCi1tF7hi)RGpionraodtds1Llp6ba0os,r1M7sAeAFyuYgP,uas5it9c1Ne9o8Sk%t,SaeOnnEd,aCrNDdosPa8ciapssloiinsnhNeod: 38t5e0s,aAygrfiicnudlitnugrsalwPerroeducptioorn eBu0reahu,S1t0udyAuDgiuvsetct1o9r84n.dTLheadartresyoMfaalnlaaguedimtesnatnwd iensrpaectiionosnasndthe
AcTvry FTropnaSnutbience SerpePrpin Dibrgpuatin PArotiReDpes,
DATECONDUCTED Retry20,2000 March 1,2000 Mah?,200 mfre16,17, 280
STUDDYDAITREECRETPOORRTMEADNTAo:GEMENT Fray Mark 1,2000 Mele Mahle Ma 7, 3000 Ma?2000 Py25,2000 may31,208
DBitsohgiealnDus, Jniam 16,1720,
Sy31,200 Ferry 19,208
FSwoiisRse1p0td DNeocvcemabner2164,,2003
Deccnber2s0,03 Desmbe 18,2003
Ainpecsonswerestybasedness kbsne.
SmaSuComleamann, BXLA. Coleman
Senior Quality Assocs Represctive
BATE 12-18-03
r2048
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REPORTAPPROVAL
SPONSOR: 3 Caporsion TLE BROS: A ilkRerotionSyvitbeNornBbc WILDLIFEINTERNATIONAL,LT, PROTECTNUMBER: 454.104 MLABREQUESTNO: 2733
STUDY DIRECTOR:
SE eABlm ob g vi5T)soer
E ds
CHEMISTRY PRINCIPAL INVESTIGATOR: 4
SACLE aytbing | Van Hoven, PRD.
DATE
MANAGEMENT:
Bra e wn oicioey f TA7mN1nt47oe 8
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nana
--_--_--
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TAOFBCOLNTENETS
PProejecttNumeber 454-104
AdultBody WeightdFood Compton. AdultNenrd iosoCpolyin.
SHS ABIES... oe
|
AGU B0dyWeg...
------
|
Offspring Body Weights...
mms
s
C2020
_--_--
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0 rProojeecteNNummbaers4s54e-i10s4
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TAOPFBACGEO?NLTENETS TABLES
TableI. rom NorborhBOsPILREOAoci00 Sec Mean Measured Concentrations(ppma.i)ofPFOSinAvianDiet 21 Table2. MeanAdultBody Weigh()fom Norse Bobi Fx Table 3. BMoebabniFeedPilCootnRseupmprtoidon(biSrtyt)MfbroFmFONSo.rthen or 29 Table 4. BoSb6wuiWmiotyrekosPfilGortTRoeesptrPoaTdtuhctloioogncaoSlhOabdsyewrivtahtiPoFnOsSf,oAmde NoBrtsherBnutbanized Table 5. MNeoarntheirngBPoobwdhoictieoPnlo(tEgegpsrLoedidcHieonnanSdtudEygwgtuhenP/FODSy.)eomarrr 31 Table 6. SurmommaarNyorotfReerpsrBoduoctitvePPeoftorRmeapnrocdeuEcgigosnSSotfyowmihWePcFkOS vcr 52. Table 7. frMeosmaaBoNodrythWeerBgohbwh9)iotfeHPailtotiRnepgroadnudctSiuornvSiviyngwOfiflieing PFOS.......... 33 Table 5. MetaLiverWeights 2 orm a NebrBaie Pil Reproduction
2021
-- Wildlife Internationnaaln,TLeed,
7. `TOAPFACGBOENTLENETS
APPENDICES
PPiroejteNctaNmubmeberrd4s5t4-i1n0s4
Appendix I. Diet 0dSupplementFOMmUISHONS crn38
APPEDR IL DietPIOPRIUON...sssennn d
Appendix IV. ThoAnalysis Of PFOSin AVShDit .cvrndl
ApEn V.. | DIOFTB E AA YO
5
Appendix VL ARdiEtPBordyOWSeGiOgUYhWCti)ltfkroOmnaNoFrOS.t. hernBobwhitere Pilot
56.
Appendix. VIL FBeobewdhCiteoPilontRse(ubpirrdmidoapSy)dturtduoymwcaiitNthooirtnoe n PFOS...cccvvrres nr 60
Appendix VII. NInodritvhiedrunaBloGbruowslsiPteaPiltothRoeOlbpseorrvgaotSiitoduncduyfarwocimltath ion PEOSA..............64
APpendix IX. Appendix X. Appendix XI.
Appendix. XIL
Appendix. XII
Appendix XIV.
Appendix. XV. Appendix XVL.
HiStODURORIEOPOBY
68
EN gg Production(ogg nidheoE /A week)fromawhPEGS
109
RNeoprrtohdeurcnBtiovbewPheiftoerPmialnotcReebpryoPdeunctfiroonm SatudyWith PFOS rr 113
MPielaontROfEfprPinIgBOoSdGyLWYUeiWgCihthHP8)ROfOrSNo.m..a NorthernBobwhite
19
RAduEltLPivIerOWSeGiOgUhYtWH(gi)tifhrOomDa NoPRrOS.t.hernBobwhite Pilot
120
OPfiflsoptrRingeLipverrWoediSguuhdtc(yg)WtiftrihomoanNorthPOSe. .rnvBobwshitee:
122.
CHANGES 0.5008 PIO0GDL..ccsres 123
PersonnelInvolved i 5eSOY...
125
cn2022
WildliLfeee Inten rnaa tion, al, iLtd,. 5.
SUMMARY
TPriojeecNtNeumibewr i454a-1m04
STUDY: PFOS: A Pilot Reproduction taywiththeNorthernBobwhite
SPONSOR: 3MCorporation
WILDLIFE INTERNATIONAL,LTD. PROJECTNUMBER: 454-104
TESTDATES:ASEdtuduxy ITpneieiromrnSiaintFa-_emFbAtrepuiebaerrolyan12nr18yt,&2290Ja,u02l00yl1030,2000
Biological Tenminatiuolny 21,2000
TESTANIMALS: Northenbobwhite (Colsvirginia)
AGETESTANIMALS: Appr30oweekxsofiaeamtthaeintitieonoflheyest
SOUTESRTANCIMAELS: T2r8i8cLeePvheensgsoaondyf,onndc.
DUosugklasvillAe,. 19508
NOMINAL TEST CONCENTRATIONS: 0, 18,62, ad 176ppm
RESULTS: mNPaoOirnMtthaieir.nnebfdooorbnwt6hiswteedewikeesrt.esTfeohxrpaeoncsodnttirooolPngFrOoSuo1p3twadeniedektasr.1y7c6oNpnocpeanmtraac.tionwasersoeuflneoca8ed,romp62,amaesndewn1e7oe6e.s
oabvpeepratr1es8nitg3n0sedoamf6et2noxtpipciemtlyawt.ee.dreetocebststcseornovcneendbtaroadatyinoWnysE.oIft,hAeftoedsdt ccdoonnscuiemntpaeidtroonne.sow.oerrnTevhe5raa0wsewlipgrahytr,ea.
||
Pacrroaenmcaeemtnerrtesomnse.easduTerhfeefdrcdetuswreiornnefthenemoaslatpeubpaorLdefnytsweryiegosfheonftcvloenirceendwteriacgthiotoantstetohsnec1a7n.y6pporsodouscwtts
||!
bWroehldeiyenwdcerioegmdhuptcatirinoentheoint17hf.ee6ceodpncptromonl8.gsrouiunmpc,upthtfeoirsgtohrwneoasvp1a.7s.Ti6hgpneiprfmicwaans1t.rewadSugecntiidoBncaiingntmmrsoeeueasptnmnmweaiatlte
cEheheostmomern1ta.pltthHeaicasatlonrpistvhaleoglhrogeodiuc1pa7l.6eThpxermaem.waissnoaaifotnseitl8eSocteineaeddlscnaunettsaraeyidoubcvoiovenebleiodmpesssttceFodon
cooe nfctentertl aotsiortneitt safsomurenecnfotor.rtl(hewBraoanasbdearudblwuthpmioatlneectsxhipenatsheeed1s{7s.06opPfRmtOSias.in teshutedsd,$it7th0efohrmko-mwsebyeakrshvweeadscph6cn3t
pms.
cn2023
SWiLldlife International, , Ltd. ieNebe Projecet Numsber 4e54-1s 04 5
This
stdy
was
conducted
by
INTRODUCTION WildeItematonl
Li.
for
3MCorporation
atthe
Wide
Iaotsercnatoionna, dLfudo.umvFicebnrtutaoreiyc2ld9o,gy20f0e0ulnttilinlEyxt2o1n,,20M0a0r.yRlaendwd21a60a1.genTehreatbeidcotgWiciallodreionnteorfmtahteiotnesat,
sWLuids.lidainedItaceorpaytoifotnh,eLfii.nasltr.epoBritoalroegficialledpunedereProsajreectsNouremdbeatr34M54C-o1r0p4ionraartcihSoitv,.esPlaouc,atMeidooenstohtes
Theoective
oftisstudywas
o
omECTIVES valsth ffcsupon
the
adultnorthernbobohite
(Collar
1v0i8r8gPiRnOsS)) oafvderiapreyrieoxdpoofsarpeprootxhiemaptoetlays6siwumkssloofrP1e9fwueoerkoso.cEifafneectSsolofnniaclAtcihdes(htehr,ebaoftderywreeifgehd,
aonfdfeeggesdcniod,nfsorutwyme,rpeeetvmabilruyaootendv.iIanblaed,dibna,tcthheubeiftfyectsndo.f adouflptreixopgosusreu1vi0vePlFOwSeornethee ausmtbe,r
10HisvoapalhoslotghiceaflfeecxtasmwipnatiaodnuoltfsseexlpeoctseeddtiosPsuFeOssSnadaduloytsseooffbflporoidngn.d sue spl were ls sed
Northen
bobwhite
(20
males
EXPERIMENTAL DESIGN and 20 females) were randomly
isibutd
oto
ane
contol
group
and tr weamen upon te resusof
garoLuCpSsD.Tshteudtyes(tWcionlcdenetrIattcioansstwieoarls,elLiedc.tePdroijeccotnsNuulmabteorn
withthe Sponsor,bad 454-103) and adidoal
to2x0ipcpimty aii. nFolflowoipnrgroevxipmdeerdiabmeyntttaelisSpaoon,sotnhre.tTehstemaotreirgiianlawlaesstrceanoalynzecdanedtsnehltefcintraeldpawuertrteyi0s,a2os,l7noawnsedr
thanorigallyreported.Theor,theactual nomi)testcocenratweorne s, 1.8, 62snd176ppm
Tm T le ma --_--
PROS Treatment Groups
Group. `NominalComes
Pens per
rain
2 3
18 62
5 5
TR 1:
--5 _us 176 s s r Lo
coord
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"10
pen.
ETahcehetreaattmmeennttgarnoducposnwteorlegfroodupdiccotsnconnetadifnivngpetiherof
idswith 15, 62,or
one ae 17.6ppm
ndoefreper ai.of PEOS, The
coanbtaolng.roupwsfed ditcomparabletothetreatmentgroups,but withoutthe addition of thetet
17.6pApduml.t nfoorrtahpeerrniboodbowfhi6tweeweekrse.exApcoosnetdoltogProOupS,faetdnnoomni-arledtieedtadriyec,ownecsemnaatiitoinnseofc1o.8,n2veanyd
Wwiitthhtohnetmeaalmeaenndtgonreoufpesm.aElsecphrtrpeeant.meAntttanedtnhdocofnWtreoelkg6r,oudpucolnbsiisdtseidofheGiv1e8psanisdo6f.2bpipds aho,vesctd
concentrationswere euthanizedandsubjected 17.6ppm .. treatm groupweremaintained
ou
grosnecopey. Tes bis i thecontrol heappropri dictsun tebeginningof
group and Weck20of
tohedtyeswte,isgthwth,icahndtfimoesthceoynwseurmepailosnocwuetrhaenivzaedlaantdesduabj5ecwteeldta ogreofsfsctnsecurpoopsny.eg`Effpercotdsuocntiaodnu,ltehmebalrtyho,
viability, Sicabilt and offpin survival forall st concertos.
bodywTeihgehatdswaerbeimdesawseurreeobsaerevetddiaaltyifoo,rmoonrtWaelietkys,2a,bo4o,r6m,a8l,be1b0aavinodrs1a1d, asidgnsaaofuelcter.minaAtdiuolnt.
FNeeecdrcposnissuwmpetrieopnefrofocramcehdponenalwlaaslmetabsiurrdseadonvderan as1e0vofefnsdpariynpgefrioomd ccaacchhweestctohnrsocuagrhaotuotnt.heLtievnetr.
weights sample
were wer
recorded cllte
for all necopsied bid. a the imeof ecropeyfo
Live, bile and blood (when posibleaalysi. Adiconal
avaiable samples of
and fear ver, bri
Kfiixdeadcyi,n g1o0n%adb,ufprfoevreendftoicrumlaulsi,nfaolbiblsatdodpert,boaldiopgocsaetesxaemi,naatnidon.bursa offbricus (ven available wre
Duringthe test,the umberof eggs inWeeks1.3and Gof thtestwer
conlildecitneedaacnhdpsnpwuarteesonrodsehdetlo,csvhlelultmeemcbgrpero,dyucotlikoann.dEaglgs
id
acnoldlsecttoerdedafnrdozseent foorrpinocusbasteioann.alyEsmibs.ryEoggvsaiildd,uraicnhgaWbeielskys,baatncdh6inDgabyset31faond37s)uovifvtahbeitletty wwrree.
pu
62025
---
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PPeriojectNNmubmebedrs45t4i-1n0s4
ThestudywasconductedaccMorAdTinEgRtIoAtLhSepAroNcDedMreEsTHuOdiDeSd n heprotocol, "PFOS: A Pilot ERvireonmpentr!StPdrooytewcdititontuhAegNeconrcytt'hserRiBegoibswothriattnieo"n.GTuihdelpirnoetso:coPlewsaiscbidaeseAdssaenspsrmoecnetduGrueisdoluitnleisn,edFiInFtRheA SSutbadnidvaisridoPnr5,aHcifzoarrCdonvdaubcatitnognR:epWodiolctfiavnSdadAgiiawticthOrAgvaniinsmSsp,eciSeusbse(c1t2i)o.n 71-4 wad the ASTM
TestSubstance and InternalStandard assigTnehdeWtiesdtesubIstteamnacet,ioPnRaO,SL,udw.aisdernetcifeiicvaetdionrsoumb3erM4C6or7p5ourpaotnironeocnipOc.toTbheert2s9,s1b9s9a8aacaedwwasasa w9h8i.t9%paonwddehrasdaedxpwiarsedatethcetiimaedso:fFexpCri.Lnoo9t2l157s.taTrto.Atnesatssseyroif hebtaedsatnmoarteirgiianlfreepoerxtperairmeyntaolf esxtparitraitionn dadteoaipfurcAiutgyauostft391,0e2.040d9%6.(TAhpepfeinndailxa1s.sTayhoofetshtesutbesttmaantceerwiaalsinhdeilcdautnedderapmrbyiotfco8nd6i9ti%oan innd sTuobcskteadnscteoirnatgehaetdtiheetwWeirdeaedjnuestremdat0o1n0l0,% Lauct.if veingrei diineEntxboal sne,dMuaps roynathne.oCrgoancreepornterdopfaahrietoytoensft b9a8s.e9d%u. Dipetthaoeryfinncaolncreenptorratteidopnusrirteyeoxfpr8e6s.9s%e.dasparspermillionactive ing(rppem 1d)iintnhedtik
assigTnehdeWiintdeemalIntarnmdtaiorn,l4,HLiPdF.OiSd,ewastireocneimvebdefrsom45236MuCpoornprraetciiopn.
an Jy2,1998sad The infernal sand
as was
"Thgerainnutlearrnamlastearniadleddewnasiebdeldsus:nd1eHr,am1bHi,en2tH,co2nHdiPteiornfsuoirolcotcnkeedSsutfoorangce tActihde WCAiSldNeo.e2r7a61t9i.o97n.]2,,
Lud fle in Exton, Maryland
Test Organioms PheasanFcoryy,-Icnicg,ht26(82L4emveensgaoaoddRo2a4df,emDaoluegsl)aspveilnles,aPrA.no1r9t5h0e8r,n UbSobAw.hitAe tweersetaprct hoafssecldimfartoimonT,ratchee fiboobmwbthietsaapmpeehaartedchh,ealppiryoaacnhdingwetrhepihrefniortsytbpriceaeldliyngisneiassionngaunshdabhldfnoombweielndutsypeed.inTahneybpirrdesviwoeurse tuesstdingt.o rAatndtohmeiszteartpoenfsacslgimnamteinotn,faorreaancdhobmind,umbIemrmegdeinesrtaltyinpgrfiuoncttoioenstntatspiroena,dshaeletpportongrlamswtays
C2076
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Es Wdilodlife Internai tional,XLstd.A mPc i rojectiNumba er454b-10o4 l
-2-
indweeexamined orphysicalfisandgralbeat.Bird a id notpesbest, terdo
toinjuryorinabilityto acclimatetolaboratory`conditions,or wereoutsidetheweightrange for thetest,
`xwepreoexscluodsedtfriomet)haensdtruadyn.gAeldilnbwierdisgwhetrreoamppr1o9x4im0a2t9e4lyg3r0awseeakscosft agenaottnes.tiSnietixaotfiotnhe(fibristrddaswyaosf determinedby avial examination ofthe pha.
Ldenttation andgrAoudpistobfpiedaswweerreeddeenniieeddbbiynpdrijviduumablebraannd,dacocnhcepnetrawtisond. eDunriiengdtwihtthsa,utrheeemumbbeeorr,f cinacuba1tidoniwnecraecmhpareknewdawsirtehctoredpedetnonvumabesrewicnggparopdeucatsioen. iAnlklmoagrakcingopelnflofroeirescanmtptflcienagoodnr Hatclingswer deniedby le bands stht heycoldbetnd 0thepretpe of rg
AvianFeedand Water
testingA.TLIhseublasbaiddsieand2tdhteoibootfhfaidnigtsweareogfifviernifnegdwaansfdowremeulraatded toeWmiddureingnsecmlaiimaotailon,aLndd.
specbiy AfgwiayIca.a(AptpenidioxI,nTabsle 2.52mo9reth4 an$5 ,fie.
1).
Thebasa
ron
connedat
est
27%
proteinand
Thebs diskcontainedapproximately 1.1%clu,derived fom foodstuffs andthe 09% lSirmoewsttohnaenudsmedaiinttehoesfnoerrmautliaotinosn,oafdtdhietbonaaslalcdailectibuym Aigweaqyui.Whilietthheislateivoenlooffcbarlceiduimnisbsuifdfsicfixentfogogr
th`sehelblafsoarlmadtiiotnf.orTthheearlesanf.adodTirhtiieson,ail5se%dt(hweiwc)aolfleiummeIstvonee i(naptprhoexdimiateflyor3t8h.e5%bCeae)iwnagsbaidrddesdftoo
|
spproximatly 3%sigh Ofipringreceivedbasa
dsibeovweitthheoumttieistsubscaoncmemeannddewdiftohoquutatle(2d.3i99o)snndmoafll5a%rdsu(pp0l7e5me%n)t,al
! |
limestone.
!
solubleWvaitaemrinwaansdsueplpelcitreodlybtyetmhiextinowtnheoif stteon(AppupbelincdiwxaIt,erTasbulpely2.).NAeltlhoefrftihenagdueltsinvorodfafswprieng raeccciovdeandcasnwyiotrhmWofmeedicIatteimoantiionntahl,Lsdd. dStranidatrdeOepesratiFnegePdarnocdewdauctse,were alyzedperiodical in
coon?
---
LWilE dlife Internationnaal,,LLdtd,.
PPreojNcteNbumebesrs45t4-i10n4
5.
DietPreparation thef`nesdtitdi.cCtosnwterroeldpreetpaarnedbeyacmhinriesntgedFdOiSe wnetreparperpairexdtwheaetwkleysbuesgadsfinrgoenlFyebrpuraerpya2ra,ti2o0n 0of0 tahnedtperstescuobtsdta1n0ctehaenbdidars apnreTsueenstdeadyoafpeaarcthspweeremki.lDlioentaacrtyciveoingnrecdicent(nwpeprtmeardaj5u)as.tetdDfioerptoaoarntflytosshfe Weekly preparstionoftestanctrl desareshowninAppendix I.
DietSampling
hereHsotmeodgdieentesi0tdyofetohseamtpelsessubfsrtoamntche iconthterodlidtiwetasoneDvalyuat0eodfbWyeceolkl1e.ctiSngsixasawmeprl leecsolflre eocmtecdas fcrhoomf
tbetop,middle andbottom upditsampleswer ls
ofthe left an collectedfrom
rightsectionsofte mixing thefedtroughsonDay 7of
vesel. Conantdtraotle Week Ito assesssaioflthe
rfaettmseubnsttgarncoeuupnddiecrsadcurtiangWesetceond6iodfotnsh. tAedtditotmioeaaluyr,eesrafmypleewstacsonccoelnltercatteidofnsr.omTthheediceotnsarmlplaensd
were stored frozen ac foranal lysis.
or
ttre
immediately
to
the
Wilde
nematonal,
Lu.
sslyica
chemistry
AnalyticalMethod We"Thetmeerntahtoidonuasle,dLfido.rtahnedanealnyesidso"fAnPaOlyStiicanlaMveitnhdoidetVwaesbraiseodupforntmheetDheotdeorlmiongaytdieovneolfoPpeEdOaSt inAvisDie"(Wide nteratonl, Lid. PrNoj.45c4C-1t10).
solutiSoanmcpolnetsaiwneirngee0xtr0act1medg0w1iH0t,hme1tHh,an2oH,la2nHddiPleuctfeudcionroaca5n0e% mSeutlfhoanniocl:Ac5i0d% (4NH.APNFOOSp; uinrteetmealr
standard) and 0.05% methodology. Ametho
formic acid flowchar i
(v4) so hat they Tl within the providedinAppendixIV, Figure 1.
cabibraton ange of the PFOS ConcetoafPEiOSoinntsh
stcanhdarrdsoanmd eaxutstriancgotsogHfortwhaee.spPamahpclaeyrsdwMeoredede1t1e0rmHiingehd.bPyerrefvoerrsmea-pLhiaqsueihdiCghhrpoemrsfoogrrmaapnhc(eHPliLqCui)d
wih 4 PerkinEiner SCIEX API 100LC TubolonSpray ion source. HPLC separations
Mass Spectomeer were achive using
equipped with a Keystone Betasi
Perkin Elmer Co analytical
column (50 mm x 2 mm LD, 3m parce size). AppendixIV, Table1.
The instrument parameters are summarized in
. Cnoopg
-_--
Wildlife Internnaatinonaal,,LLtdE. -
PPreojieecNtNeummbbeedrs44t-i1o0n4
conaiCnailnibgra0tio.n s0tan1d4a0rHdPs0FoO1fPFO(iSiperrmeaplarsedainndear5.0% smnedth0an0o5l%50fo%rmiNcANaOcpidur(ev)w,aterrosgoilnugtioinn
(cWonececnktr1a,tDioanyf7r,oWme0e.0k0,04D3a9yf0oa0n.0t0h4e39smagmplae.r.e.-(exWtereakcio1n,sDte)y,w0e)roeran0a.l0yz0e3d1w1it0h0t.h0e0s4a3m9pmlgs, aTih.e
same ndmostprominentpkrespons orPROSwaswilzedfomoniter PFOS all irc,galt
ccoonmtproonle,natnsd.sLtuidnyessamrpeglreess.sNiooneaqtutaetmipontswwaesrmeagdeneetroatqeudaunstiinfgypPeFaOkSaoreutrheespboanssiesrofoisnd(ivPiRdOuaSlisoemremrailc.
stsatnadnadarrdd).vAernsuesxatmhpel.eroesfpeactciavleibractoinocnecnutrrvatioinsrparteisoesnt(ePdFiOnSApp:enndteoaIVl,stFainudraerd2).oTfhtehceocnaclciobtrmattiioonn
osfamtpelsetssiubtsotatnhceeaippnlitchaebslaemipnlceasrweagrsedsesitoenremqiunaetdibony.suTybpsiciaulinongcthhperakoarmeaaoeftsplooouwsagearadrthioisaghomfetsvheel
calibration sandrdsaeshowninAppendixIV, aepreseinAnpptenedidxIV,Tule 2.
Fi3gaodu4, rrespeectivsely.
Examplesofcaleaions
calculTahemsetthhopdrloidmuicttooffqtuhaeslioawieosnt caLliObQra)tiofnrtsthaendWaerdeakna,lDyazyed (a0n.a0l0y0s4i3s9waags s0.t1L5)1a047t9heoamvear
diWleuetikon6,faDctaoyr0oafntahleymsetsaxndbtlhankssaammpplleere2-0c0xt0rLaIciCo)n.seTthweamsseteatLt1O4Q1 hpfoprmtohaeiWedceakl1e,lDaoda78aynhde
productof he loweststandardanalyzed (0000351 mg ..1)an the veeldlnfactoroftemats
blank samples (4000LIK).Examplesofcalculations represeatedin AppeadixIV,Tabl 2.
i
interAfelroenncegs.wiNthothientsearfmeprelneceasawleyrseso,bfsoeruvremdaatrioxrbalbaonvkesthweereLaOnQaldyhzreidngtotdheetsearmmpinleepaomsliybslne
(Appendix IV, Table 3). Figur,
A typical ion chromatogram ofa mtx blak is presented in Appendix IV,
and2A2v0ipapnmdieatis. am(pWleeeskwe1r,Defaryie7;d Westek0367,9,Da7y9,0a1n2d2t2h.e0spamppmle.r-(cWxterakct1,ioDnasye0))axd1s7n6s,y8e7c9d cprooncceudrucraalllyrewciovtehritehse osfam1p0l5e%s,to10de4t%e,rm1i0ne7%thaeamde1a0n2%p.roceTdhuersael vraelcuoevseryc.omeTshepomdettohoadcyhiesladmedplmeeeatn analyzed or reamlyzed during he defisitive sody (Appendix IV, Table 3). Sample measured
cna029
_--
a Wilcdclifei InteT rd natim onal, L. td. . Prsjc. tsNumbier45i4-104
is
consenraonswere no pcan chromatogra
coofmeamciaedtfiorfotrthifirceastpieonctiisvermeesaenepdrioaceAdpupreanldriexcIoVv,eriyguorfteh6at samplese.
A
HousingaadEnvironments Conditions
the
Housingaad hsbandeypracticeswereconducted50a Noionsl ResearchCouncil (4). The adultbirds were
adhef0teguidelinescuablishdby housed.indoorsinbares of pens
ampapnruofxaicmtautreeldy 2b5yX G1eocrmgi.aThQeupaeinls FaadmmslopMiannguflacotrurtihnagt r(eGsQteFMneMoidnel beNiog.ht r0a3n3g0i),ng fmreoamsu2r0in0g
26cm.Thepens weeconstructedof testlayoutispresented inAppendiVx.
galvanizedwire
mesh
andgalvanized
sheeting.
A
agrofahe
period
Eachpen was quippedwithfeedandweertroughs. wasplacedinthe rough forcachpenandpresented
Weekly, sufifeedfo the footing to th bids.During the fingperiod
addditeioanal eiceedswaaystwo epirgohveiddeapnotaabdldeedatetr(hgerneurgalslysesvpreys2d-c3 d.Wysa)t.e troughs werechangedsndwater
excessOivneldyisbtiurrdbasancsesso.cTiahteedwviertshgtehitsesmtpuedryawteerientmaeiandtualitnendoirtnhterhnebsotubdyroostmuidynroorodmedrutroinagvtohied choanudslienogfstyhseifeemstinwtahs2e2s3h&yr1o1oCmw(aSsD)dweistihgannedavervaegnetruelpafti0ve1u5riootm aoifrv5o0lu+me1s4%ev(eSrDy).hoTurhasneid replacethemwith freshsie
photopTehrieodphdoutroipnegraicocdilimatthieoanduanldttnohrethteersntbwoaswi1e7:hrooosm owfalsimghatlpsearidnaeyd
by to
atime clock. The indiceegg laying.
Throughout he tes, the bids received laminationprovidedby orescea gts
a mean of approximately 188 ux (~ 17.5. ht closelyspronimatod nonesulght.
candle)
of
Observations
Thetestbindswer celimate th inition ofthe test. Dung acclimation,
ilies all bids
and studypeas forspproximtely 6weekspriorto were observed dally. Binds citing aboormal
bobseervedhodradialeyiflvoartsiiigongspoohfysx ircal ni jorrcssbuwi oerrmeanlotbeuhsaeviofr.rtAhditdeotn.alDliy,nogl tohfefsstpruidnyg,waelrlaeuoblsebrivdesdwdeirey
2030
-_--
RiI Wioldslisfe Iintiernational,RLetnd. Pr.sijvctiNiusmbbeiri45t4i-1o04d "16+
fcliionimcablacohbsienrgvatuinotnisl.approx1i2mweaektsoeflagye.A recordwasmaintainedofallmoralesand
Adult BodyWelghtandFeed Consumption
terminAadtiuolnt.Fbeoeddywceoingshutmspwseiroenmferasusrcehdpaenetwaisamienas,uroendWweeeekksl2y,th4r,o6u,gh,out10t,he1.1atesna,tadFduolotd
adcditioonsndiewstaasduddeetdemdrumripinnegdbttyhewweiiegehoki,nagnntdewfeeisghliyngltohedffeeeddeeroanndDeryem0a,ireicaofdindgtaht athmeoeunntdoofftshaey
mfeoeudnitnegpdefroidoedr(sDdensi7)g.nAednwiatthmp2 t"Tweaosdmaavdere"folmp.iniTmhiezeafmooudnwtasotf afgeeebdywahsetebdirbdybtyeibnigrdes xwearsanloyt
feqeudacoins,umspintcieotnhiswparsetsendifeededawsaasnncoarimmaaltyesocfatrotealdfeaenddmcionsseudmpwtiitonw.ateArlslnrdeexcriet.nbiTirhdersnewfeogrree,
euthanizedtwodays afer cleforWaeekt20,
he beginningof
Week
20.
Theretore,
no
eed consumsptitmtiewone
:
AdBlooud Colllecttion Week 2A0tfhoertcointeroolfgrduolwatnerdmin1a7t.6iponpm(eadi.ofgWreuep)kb6looord1s.a8maplnedsw6e2rpepcmoll.e.ctgerdofuopsm;baelgisnunrivnivginogf bBienmda,ywtheelntplosesti,blea,ndprsior twu oacsubsm taonresdaf.rozeAnlalndbsoioppdesdatmoplCeesntrweeArnessleyptacraltLedboirnstoorsiecr,uam.snfdor possiblesays.
AdultNecropey nd TisueColcton dislocaAtitont,hneeccornoclpussiieodn,oafntdhseteoxrpeodsfurroezpeenr.iodA,tatlhesurtviimveinogfadunlectrboiprsdys,twiemeeesuthwaenriezecdoblylceercvtifecoardl siasmtopplsetshoolfoggiaclalbelaxdadmeri,nalitviero,n0praonvden1t7r.i6cuplupsm, kai.dogeryosu,psbroil,y)agonndadpso,ssbiubrlseaanoaflyFsaebsr.iciWuhseanndavaadiiapbolsee, iHimsteopawtehroelofgiyx.ed Winhe0n%abvuafiflebrleed,fsoarmmaplliens. oHfibsitloeloagydslivaer wmewreeprstlohrieedpefdrsoz0eEnPaLnidnshHieprpneddont,o VCeAntfroer feAantalhyetriscaalmpLlaebosrwaetoerisetso,reInf.rfoorzpeosfsoirbploteeanltyiaslisan.aAlynsiysr. emaitnissiuenpgot fixed or hisopstbolaognyd
n2031
---_--
L Wildlife Into ernational,LLm tdd. a PPreoijeec, ttNNuammbbeer4e5s4t-i10o4n ne
EgColeantdStoorange collectFeiddsailynidrdoumrientpaesesvaennddsaoyrpeedriiod bceoglidnriongm.uonntlhinsceucbaotnidodna.Ty hofcoWledcrkoo5mowfatshaeteitwoeree
atamean temperatureof 13.1C +0.1C (SD) with ameanrelative humidityofapproximately 72% 4%
(cSoD)n. Gsrioud1psesoorefeeldgogs wereidentifiedbyanalphabeticlotcode. Alleggslaid during theweekwere
CaAnttahdnedelInndciuofbn tahteig woenekly interval,aleggswereremovedfromthecoldroom,countedandcadled wCirtahckaedSopressbdoKoirnmgal(MeogdgsewlNeor.re3c2o)redge.dcaanndldiinscgalrduemdp. Atolldegtgcstteogbeisnhcelulbcataecdkweoresbunogoartmeadewgigtns. fpoosrsmiablildiethyyodfegpaasthinoganenaicnoingthatmicnaabtiinoent wpirtihoraci0ciunlcauibantfiaonf.oraFpprooxirmamtelaygweloswdoausres,gehnreeyrdtuecddetbehey cboomwblinaitnhge2b6aseooffpthoetaistsiiguhmtpecarbmiannegt.anatead 25mlof37% commercialgradeformalin in porcelain
the teAmpelraetgugrsewnaostdmisicanrdeeddweart enplavceerdigne37P.e5e4rs00CI(nScDub)awtoirt(hMaondaevleNraog.e wSeP2t0b)u.Ibttmhpeeirnsctuibaoofr
fa3n0.a6:n0d.1blCa(dSesDt)halaptriovdeuhcuemdidiatmyoilfdspbrroesaitmhaitneglayc60m%o).veTmheenitncduebsaitogrnewdas0equcilipmpiendswtietinhrapcualbsioteotr
temperature ndhumidityvariationduringincubation.In ordert preventadbesion oftheembryo tothe
|
srhoetlletmheembrgasnef,rtohme5in0cuobfaftoorfwvasearlasoienqouiepdpierdewctiitonhafno a0utoomfaftiocf veegrgtircoatlaitiotnhdeeavpipcoes,idteesdiigrneecdtitono
(Ctlar of rotionwas 100)every wohousthroughDay21ofincubation. Eggs werecandiodn
Day ofincubationtodetermineembryoviabilityan onDay 21 0determinecabryosurvive.
|
Hstehogaad Broading OnDay21ofincubation, tecgeswereplacedinaPetrsimeHotcher(ModelNo. S68)and
| i
alclolwiendgtsosheaptucrht.edPebdyigprreeebvaaskepts cooefnosrtirnguicnt.edoEfgggsalwvearniezendotstreoeslaweidrienmtheesbhawtechrees,usTehdetoavkeeregpe
i
wfaesmpiesrearde10i3n3.t3he B0a0chiCng(ScDo)mp(alrattimveenthuwmiisdi3t7y.o2fp0pr0oxCim(aStDe)l,ya7n7d%t)h.e average wet bulb teperstare
"2032
-_--
Wilidllaiyfee Iinntteerrnnaattiioonnaall,,LLttdd.,===~~ `Proeject tNumbwer 5a4-10s4
"1s incubAatlioln.sTchienggrso,upbnobdycwegehtgo,ft0hedsugrtisvhienlglhs cwelrirnegmobvyepdenrowmshdeebtecrmisneda.nDHaatych2l5inogr2w6eoref lpaergtbiaanldecdofnocresidseinoinfircasiopnibnyipnebnrooofdirnig npeasndutnteilnaoprvoisicmyaehloyuse5wdeaeckcosrdoifnagge0.tAhelappcrloiprnigs fwoedruenmroavteeddd1iatgwiethloiugthtthpeeands,dwihenrofthe4ywseuprpelehmeonutsaeldslpimpersotsoniem.aeAltys7pweecekssi,aTelhye12atwchelinegosfwkaegrsee tehuetbsavneizaegdewbiotdhcyawrebiognhtdiboyxpidaeracnudtldpiseposoefdaolflbuyviinvciinneraotipoin.nTghwoessodfeftseprrmiinngesde,lenctded hfeorbbliododwenred suesamplingweesrefonfollowingncrpeyand te dispsedofbyincineration
(ModeHaltBcTl3i5n0g)s.wBeacehpbeunsmeedaisnubraetdesrpiepsrooxfibmraotdliyn7g2peXn9s0maXn2uf3accnrehdibghy,BTehseccoxnStteenell Cwaolmlpsaannyd
ealivngsnofiewxiczrhepmeeensdwhe.reThceonrsmtorsutctaesdinoftgheabvraoiozdeidnwgicormmpearsthmaenntgoafveaaiczhepdesnhweteiroeg,stFlfooorsnwearieoaf
Otfeamgpee.rateBroofaadpinrgowxaimsadtilsyco3n8tinC uedomontcheetbaechoifnhgtscwienrge idetetrhmebinieddstwoebreeaoppfrsouxfifmicaiteenlty3si0zeda1y0s
h1e0rmCor(sSuD)ltwei.th Theavaevreargaegerealamtbiivceahtumoiodimtytoefm39p%e&r8i%nt(eSrD)o.omAlhohuasitneglbigrsowaedrssvraesmo2v8e6dfr4om
sbpropoodniingmpaeinlsy a1nd2mraXns1fe2r,dtwoifltihghtcpeensia bperiogxhiomaftselpypr5woexeiksmoa1ftla.y.EFxatcerhnialgwhaple,nlmeesrssendd
celing of schpnwerconstructedof wirmes.The phooperiod atimeclockat 16bursofightper oy.
or the
clings wasmaintained by
| |
OpinBloodandTimeColton
Pri to catboftaheaoffsprinsg, bood sawmerepcolllecteedfsom 10 offigirnciancgh
|
tr5e2adtsmiepnptgerdooupt.heAClltbrlooAdnssyatsmwLepraelserpearsatse,dosi.nftorspeorsusmibalnedahneamlyasceyst.esA/pdlaitenltas,,stosruedefsrofzoenm,
atvhea1il0aboef,spsramipnlgesinofegaaclhlgbrloaudpdewr,erievcro,llpecrteod vforeins,topKaitdhoelyosg,iebraaienx,amgionnaadtsi,onb3unrdsoaflyFasbsri.ciuWshaennd
aHdiisptoopsaetthoolomgye.weWrheenfaxvadibilne, 1s0%lebsufffieldefaonrdmavleirn sanudeswheirpepesdtotroefEPoLeiannHdesmhdipopned,foVCAentfroer
Analytical Laboratories, o. forposible sys.
.
"2033
_--_--
L WildT lifeHIOntmeArniaotinoannal,,tLdtd.. 19-
PPreoijecttNNeummbbeerd4s54t-i1s04s
Static Ansyses
statisticUalploynsciogmipfliectaitodniofffrtehnecteesbte,tawneeAnngarloyuspiss.ofDoVnanriea'nscem(uAlpNlOeVcAo)mpwaarsipseornfporromceeddutroed(e5t,e6r)wmiense usseid 1g0conmopfairheftahbeisrbcvreedeadtirfnefaertcemnecneets.meSaenmspwlietihttshweecroenttrhoolignrdiovuidpumalepaennsawnidtahsinsecsasctheoxstptiesrictal wuerpe,exeaxmcienptedbwoidynganDdulaivnerew'eimgehttshowdhfeorllotwheinsgaamrpclseinuenistqwuarsetohoitndtirveisdsutalombsitci,on,PeTrswootasgeesdoetfa souniyntliocolkeadnalaybseosdwyeweeicgohntdauncdtefdeewdictohntmhuembpoidoynwdeigthft oanmdeheeficrosats6wumepetkosnodfatte.sOtneysaenodefanvyes rlalitmrecnet-gersotumpesnat ngrdoeuxpasm.inTehdedsatcaofnodrtshee offlalndaalytsiiosonnloyfthveastludiyd, t2h0ewceoenktsr.olD3u2sdae1t7'.6spmuplmipal.e comparisonprocesswasnotconsideredspprpesietocompath controlgroupto singeream cgornoturpo.l`gTrhoeusptaundedntth'es 1T7-.t6epstpwmasaui.serdetsoemnatkgersotuaptiwsetirceaclcoommppaarreids.onsinthoseinstanceswhereonlythe
L Adu1l0t,Bo1d1,yaWneidgahttad~uIlntdtievrimdiunaaltbioond.ywSeaitgihsttwiaalscommepaasruirseodanstwteesrteinmiatidateiboen,twWeeeenksth2,ec4,on6,to8,l
adduitpio4n,ndeatcishitcraelcaotmmpenatrgirsoounpsawteeraecmhawediegbheitnwgeiennterhvealcobnytsroelx orro theafnids1t76.w6epepksm,aI.n
reamsgroupforweeks8, 10, 1,124 20
2. AdulextFameiendeCdobnysupmepntwioenek~lFyedeudrciongnstuhmeptteiton,eSxtpartiesstsiceadlacsgroammsofpfeweaedrperemrbaiidredsbpeetrodweancyawtsahes
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wceorntermoaladnedbeeatcwheterneatthemecnotngtrooluapfdor17w.e6epkpsm1tah.roeuagmhe6.nItgnraodudpitioornw,seteatkisst7itchalrcooumgphar19i.sons
|
3. EernepLraoidudct~iTvheepenrufomrbmearnocef,gdastatiakdepnferrofmewmealeekpergtgraesattm.entgroup.Fortheevaluatiofn
4. ViableEmbryos -The umberof ivecnbryosdetermined aDay 11bycandiing.
S. EggbsyCtrhaeckneudmobfeErgogfscLgagidai~dT,pheernpeunm.ber ofeggs determined by candling tobecracked divided
6.
ViableEmbryosofEggsSc ~The umberofcnbryos at nuofmeggbs sce,perrpen
he Day 11 candingwas dividedby the
-
72034
---
LWiLldlife Intemranattiioonnaal,, LLatdd..
PrsjctNNuembmere45s4-i10s4
"0.
7. LivWe3asWdeievkideEdnbbyrtyhoesonufmVbiearbolefEvmibbrlyeoesmbr-Tyohs,pneurmpbeenr.of iveembryo ftheDay21cndiing
8. Hutcdhiivnigdsedobfyt3h-eWeuemkbeEmrbofryvose3~-Twheekuemmbbreyorso,fpheirtpcehning removedfromthe batcherwas
9:
14:DayOdSurvivosofFtclings ~Themumbeeof numberofhatching perweek,bypea.
14-day
odsurvivorswasdividedbyte
10. Hatchling of EggsSet
weebykpe,n.
~The
numberofhatchlings wasdivided bythenumberofeggs setper
11.
14:DayOldSurvivorsofEggs Set~Themumberof
numgsbseteperwreeko,bypfe.
14-dayoldsurvivorswas dividedby the
12. OfsurfvivsorBp sowedrryei Wmeein agshugtre~' dTbys hpeacgersoaulpbpeodgyrowuepi.ghtsofsurvivinghatchlingsand 14-dayold 15.LivoerfWoeifghft -podrifvwiiedsunkalg5i.verSwteaidgshttcslwceormepmareiassouursedoaf taddualtttievierawteiognhtasnweartemeacdreibnaytsiooxn
betweenthecontrolandtreatmentgroups Juvenile lveweightswerecomparedbyfest
`group, witrehgarodtu osetx.
Adultnothembobwhit
RESANUD DILSCUTSSISON weeexposeto PEOSst nominaldiary
conceatationsof
1.8,
62sod
w1i7t6h pthmweait.mfeorntgperroiups.ofEa6cwheterke.atmAecnotnntdoltrh cvontreoclgnroouprceosntsedisdtiectd,ofwafsivmeiptaaiinoefdbcirodn,ahreenetdy
|
ith nemaleandone femaleperpen.Attheendof Week6,adultbirdsinthe 18and6.2ppma. est concentrationswere euthanizedandsubjected 1 gossnecropsy. Testbirdsin thecontrolgroupand
i |
1h7e.6tepspt,mtaw.hiccahttmiemnttgheryowuerweerlesmacitnhtaaniinzeeddonntdhessubpjpercotepdrtiotgdrioesssnueaopteey.beginingof Week20of
i |
Analytical Results
PresencNeonofecoo-fctlhuicnogntsruolbssatmapnlcaeestshheocwheardascoteyriisntdiiccarteitoennotifotn eimpereosfetnhceeteosftthsebtseastnscuebs(tTaabnle a1),ofDihce
swaemrpelaensawlyezeedco1lleevctaeldufteotmhethhomo1g8,en6e2itaynodft17e.t6petmsu.bs.tatnestcoantcheentdriateionnsdon1 vWeeriefky1,eDstsuba0st,aaynacde
concenrions. Meansand standarddevion foe he thretestconcentrationswere 18 0.13 pms,
n20as
SEEy
LWUilAdlyifee TInntteerrnnaattiioonnaall,,LLetdd..
PProejiecetNNuembbeerd4s54t-1i04n
a1.
604065ppm a. 0d 17.6 1.46pom .. respectively Thecoefofviaricatioinweeren7.2t%,1s%
1(0dA8p3%p,IeVr,ensTpacdilvieel4yx).. SaTmhpelseescvoalllueecsterdeprdeusreinntedWe1c0k0,6,9D7aysnd0o1f0t0he%tosf noovmeirnsytceostnscbestiaainocnes
concenirion orthe 1.8, 62ad 17.6pn. dietsbdmeans an sandarddevinionsof 20. 0.092
PPM8.3, 6.0: 067ppm a. and 163 0.608ppm i.respectively.Thecocfiofcvriisctoonwtesre
46% 1%and3.6%,respectively. (AppeIV,nTadblie 5x).Ansys
Thesevaluesrepresented 111,97and95%of ominal concentrations ofdictsamplescollected romfeedersaftebeing held a ambiat
temperature for 7days averaged 100%, 103%aad 8% of heDay 0valuesfothe 1.8,62 3d 176pn
test concerenspecttivrely(aAptpenidixoIV,nTabsle,6). is shownin AppeIVn, Fdigiurex.
A typical ionchromsofogstrsaamplme:
Mor and CtlsesaObslervatsions Noadultmortaiie occuredinthecontolgrouporinaayof he teament groups dinthe
courseofthetest. SevernbindswernedwithBes orfotlesionsan feather os a eslofpen awsseoaciaatleodswiptehathmeaitraiggsre,ssiAolnldtureinrgbtihdescowuerrss oofrtahelteistp.eAarainncciedaentdalbcelhianivciaolrsfigonr,thlaedmeunreastsi,ownaosf ores
AdultWBhoedyncWoemlpghatred thecontolgroup, therewerenospparettesmentreli effectsuponbody.
|
weightat he 1. and 62pm .. testconceptionssnsaydiffrencebetweentecontol groupand
hose two resent groups were not saiically significant ot ay of he body weight nerves.
However, thee were retment late reductions in mean body weight for malsa the 17.6 pom ai
treatmentgroup. Witheextcepthion ofthe We2bodeyweikght intmeearnmvaal blo,dy weigaththte
17in.t6erpvaplsm(a.Wie.etkesst4c,o6n,ce8n,tr1a0t,io1n,waansdst2at0i,stFicoalrlfy(epm<a0l.05b)oddiyffweeriegnhttf,rnoomntehoefcotnhterdoilfgfreoreunpcaestaolblsoetrhveerd
:
between thetreagtroumpsennd the consotupowelr statistical signfoiranfyoiftcheaenratl
monitored during the study. Mean body weight measurements are prescoted in Tobe 2 nd individual
odyweightmeasaueprreseentmedienApnpentdixsVL.
n20a6
_--
LWe ildlife Ientenrnaotinona,l, LLted.,
ieNePrbojecet Nusmbetr 45i4-1n04
2.
FeedConsumption
HoweveDr,uwh1e0necxocmepssairveedwa1stthaegceobnytsoolmgreobuipt,tsh,efroeodwecroennsuomtpatitomnewnats lavtaerdiabflefbestwuepeonnpfeeesd.
consumption t the 18 or 62 ppm ferencesbetwenthe controladthe
ai. test concentrations. While saisically 18pm .. testgroupwerecbsrvd ding
significant Weck 82d
(7<00%) 6of he
strudy,etheseladtifhfieigeencssetsweedhrosnet.cAotnshiede6r2edpcna.n. ttertelcaotend tduaeinot,hehelraecwkoafs8csoanicscearltyosirgensifpiocnasnet
Hroedwuecvteiorn,inhefseeeddifcfoenresnucmepstwieornedturoidnogsWereeskpo4ns1invdeaWnedctkhes6iwgnhiefinccaonmcepoafrtehdetoaptphaerecnotrnetdoucltgiroonuapt.
coWneseukmp6tmiaonyibnathveecbeoeinlgcroonuspeqatueinsceiomfetihnetefrivcaltaactotmhpraerewaostatrelsateirvveleydltgWeicnckrease7i. nThfoaod,
atWeek6, altetmentgrou ut wer actually highertan
feed consumption rates servedst Weck . As
weresignificantlyreducedfrom thecontrol res thereductions infeed consumption
level 62
PP 8.3.werenotconsideredfobe reatment elated.Therewas eatenelated fstup feed
ecaodcnosnusamtu1p7ma.t6p1pi7p.tmo6 pian.oio.tenis.tc testo concn entrc atwihoe newnan csocmot pnasriser tdetna ottthhrt eocuogni htoruoto ltghreon sutpu.dTyahnedreitdiuscictailoilyn smiegansiufirceamnetn(t7s<a0r0e5s)horwomnithnecToanbtlreo3l,garnodupfoatodWecoenkssum2p,t3i,on4,6m, e8ansd 1s5. buMyepaeennfmeaeedecproenosseunmttpetdisionn AppendixVIL
Gross Necropsy wereeuthaniAzetdatncsoudbjoefctWeedek0 6r(oDsasyn4e2cr)o,paslyl.aAdduultlbtibtisdisnittehe1.c8oanntdrol6a.2npd1p7..6pptrmesatim.enttregartomuepnst
agrnoduspswuerbemjationgetraoicsnsetdnoecnertohpdseya.ppWrohperinactoe dmipeatrsuendttioltthheebceognitnronligngroouf ,Wteheekre20waasnadnwienrcertehaesneediuntchiadneinzceed
i
irneatmheennt uelamtedob.fmAealllretshewriftihndsimnaglslobtessetresveidnwethreec1o7n.s6ipdpemredt..obgorionucpidehnattawlfso croentsmiednetr,edNpeocstsiobplyy
fidingswereportedin Table nd AppendixVIL
10Stjuudvyenoiflfepbriirndgs wfoerrecaacphpreosxtigmraotueplywe1r2eswuebejkesctof taoggeraostnheecriopmsey.oNfeccurboapnsssyisr,eveaAledsuobgseatmoptlhereoef binds in cach treatment level with some feather los. Additonal, a single bird in the 6.2 ppm a.
2037
-_
Ly Wilde life mInetrenrnaattiioonnaal,,Ltd4..
PTroejeectNNuembbere45i4-s10m4
z.
restmatgroupwas noidwithsbasionsonthe incidtoetrneattmaenlt.
np. All findingsobservedwereconsidefroebde
Histopathology
Lightmicroscopicexaminationwasperformed onselectedtissuesbya bowrdcriedpathologistat
pErxopveerirmiecnutlauls,PgaatlboblloagdyderL,abaodriaptoosreietsi,ssuInecs. aHnedrnbdonu,ofVFAas.bricsSieucsti(ovnsenofalivve,abriwaienl,ekcaiodlnlbeeyc,tledgefornoa)dm,
adulttsbitsad rom 10ofspring (aproximatly 12weekslda chaps) romechts group for
perxoavmeinntaitciuolnu.s,gNalolblleasdioenrs, coovnasriyd,erberdainpaosnsdibbly rueloafteFrdabtroiscitruesaotmfendt awtermeanloatenddfion allivser,,okritdnheiys,
offpringat say ratmentrelated
oftheconcentratiteosntesd. Additonal noteinadipose sscofadultmales o
ther wernolesionsconsidered males sa offspringofboth sex,of
obe i the
testesofmaleoffspring.
tubulTeesdteis omefrtwomodsutlcmoanlseissenat thwei17h.6pposptmeprio.dutcsttivceoncpehnatsreatiroengerxetsisliboint,eddencoermsasldspehmyisniilfoegrioenasl mPahyenboemeinncoind.enTthaletoocceuarmeencnet,obfuet treeartmreeagtreeslsaitoendfeorfatlkcmoladlsoitbehepre17c.l6updped,Tah.etfelsltepsholgrooguyp report proviinApdpeenddix X.
Tisue Analysis
Theanalysisoftheeg.bloodandtisuesamplescolceddingthestdywerecondcted by
ExanydgevnrReasneaalyrtcihca(lfoermserslyakrnesowpnoarseCeinntr"EexAtnraalcyttiiocnaloLfaPbootraastosriiuemsP)aenrdaorreraeptoartnedsseapafroatfneloty.me BQluoaoid
|
|
serum and
O41".
quail
The
liver
cay
for analysis using HPLC-E
componcas ansyial
lectrospray/Mass Spect
ress ae reporid
rom
in
e
try.
"Ext
Centre
ction
Study Number
of Potassium
i
BPleecclruoosrporaoyc/aMnaessslSopnescetomfetormy.CEegngtrMeeSatburdayoNeu,mbAelrbu2m3e.n0,65"a.nd Yolk for susysis wing HPLC.
cro0a8
_--_--
P Wildlife Intee rnatinonaLl, Ll td. e Projecs t Number454-104 Em
ReproductiveResults Ther were50apparent reament elstdfftsanogproduction tanyoftheconceptions
tested. While egg produwcats hiigohlny variableamong bens, egg productaittohne 1.8, 6.2 or 17.6ppm
co#n.c.etnesttrcaotnicoenntsrarteisoennstwedaisn Tcaobmlpear5.abIlneditvoidouraelxocgeepdreoddtuhceiocnondtrtolrgerorueps.eMnteeadinneAgpgppernodducXt.ionby
viabiliWtyh,ebnchomipa,reb1attchhicnogntbreolhgronupd,strhieveawbeirltey0atstphaer1.e8, 6r2esoern1t7.lptpdmfi. tIveso,nemWbhriyloe chonecreenwtraastion,sigsneirfeicdaanctt(i<on0w.a09s)rdeedufoctfionrincthge esiunmeretoffoviiancbulbeaiobnryaosisattvheel,1P.8epnpPm 2a.o0fte7hte w1r.8epofmo.e.blaevtecl6hdinnogdl1y4-udraiy odhsewukrtavtegih 1v.w8eperpmcsao.lletfmrentcugrboautpi.oHno.wAesverr,ewahletnethree orbesperrodvuecdtiavaenyndopfotihtescowenrceenextprreasstteids.oenass.pTehrceerntawgaess,slnioghtsatuistticaolnlyinsicgnbirfiycoanvtidbiifffeyreantcetshewe6r2e rPesopmonasii.vetesatncdwasono nconswichdeernecdeormepatamernetttroeltahteoedc.onnRtoleHpowervdearto,atahidrssruuemdumcactrioinztewdaiisnnToavbtldeoe6se, Reproducivedtfo individualpensi presentedinApeadisXL.
OffspriTnhgeBroedwyeWeei20gahpsparent remmentete fectsuponthbodyweights ofBatching insayof
he tment Juvenile birds
rope, Therewee lo nthe 1, 62or 17.6ppm
80spp a. eam
reste group.
relied fectsuponthebodyweighof Offspringbodyweightdt preset
in Table 7 andAppendixXII.
i
Liver Weights dul Wivherewnecightomhept1o 8tahae6cr.o2upermlg. rethcoeorneowcueatrrptnonos,ppoaornenfer maleaedultlvsatredweeeifghtnsoananmtyaofe atdhuelctonicveenrtwreaitgihons etethde.17H.o6wpepvemr,i.beterstwcoanscesnatiraatiicolnl.ysAgdaulitfimcaalnst(ipn<t0h.e05c)onterodlgurcoutiphniaeodsnmmeasle vei.rtewsteicgohntcoefn3t.i4o0n2.grWamhsecocmomppaarree0ddtomtehacoinvteorlweglrgohutp,ofth2er.e52w7egrreamnso afporpamraelnetsreastehent17e6laptemd effects on juvenile liver weight at nyofthe concentrations ested. Mean adult and offiprng liver
72939
_--_--
Wirldaliefe IInntteerrnnaattiioonnaall,,LLtedP d.. ei Project Nums ber454-104
5.
weights are presented in Table Individual offing vrweights
8. ae
Individual presente s
dot vr weights Append XIV.
re
resented
in
Appendix
XII
Norte
bobwhite
were
exposed
1
concLusion PFOSatdicary concentrations
of
18,62,
ad
176peai.
faod6ikoss.13Twheekcso.nNroolwgeroaumpesoatde1l7at6edpmmoria.lcaetomroevnertrsoipgnaswfesmcisswienreomsretwdictaoyrof
tchoensuesmtpcioonnconrirliavdeorwne.igThhterteewe18ead06.p2ppaprmena. tettmeconntccenltrtaetidonfse.ctAsdondbiodoywtoehiragehwlti,ryefao,od
scpopnacenrnttorne.atmTenhtersetweerfe5f0ssponrfeemaleebsodeytweeisgthetdoeflfiver awneainaygtrhehptreec1e7t.i6vpeopmraut.neosn
meadduringtestudy orsnofhe concentrations std
icn tohe`Wn1h7e6mnfcpoomprmmphpae.r.1e7id.tGeoposttrhmeencnoitn.tgrsorlmog.reoupT,ghtrehoreuerpewwwahasesaacsosiimggpnnaiiffriieccadannttorteeedaucmcteioonnttienlgmaretueapdn.mrTaedlhuecrbtiowodnyawineiifgeahedt
siagvieficbaenntrreedluicedonfointmheeatnatmveern.eigHhitsfooprastuhllogclleeoxfamihneat1i7o.n6 pofoscal.cerd teisttslmelveoletrnonvemlteyd
reepresie o0noefd atemsi.clrtBasseedfsoprnttohedetslloefsiinshe d1y7,.6phepmm oi.bteostgsroauoptthstommyeshvepeocn
nonebobbi exposed o PROS itediet or 6wcks was 63ppma
|
n2040
_--
WildlifeT Into ernam tiona al,tLetd.. %-
REFERENCES
ProoijetctNNeummbbeerd4s5t4-i1o04s
1 USEunSbv.divrEionsuivmoienrnotnElm,PernoHttaeaczltairoPndroAtgEevecantlciuoyatn,iOoAnfg:feinccoyW.fiPledsi1t9fi8ec2i.daenPdPreosgAtrqiaucmaistd.iecWAesOsvrekgsosagmnieonontn,DGuehi.delsinems, FI3F1R0A 2 ARStmaenerdiarcpdasn.rSVooScolite1ted1y.s0fwu.oritTPchehAsivttiaiadnniegSiappevnchdPiieAes,M..at1e5ArpSaTpls. Sta1n98d6a.rd SE1t0n62d.r56d.PraA cticeuBfoocrks Coofcwhctotisng 3 Me&rCoc,Iknc. 1991.TheMarckVeterinaryMoria. Mer&cCko.Rab,NJ. 1832pp. 4 NaWtaisohnianlgRoens,eDaCrc.hNaCtoiuonncallA.cad1e99m6y.PrGeussi.de13f5orpp.theCareandUseofLaboratory Animals. S wDiumtmhettC,oCntWa.. J1o9u5r5.AmAerMuSltaitpsl.eAsCsoomcp.a5ri0so1n0s96Pr1o1c5e1dure for Comparing Several Troms Dournner C:W. 1964. NowTablefor Mille ComparwiitsCoonntrosl. Biome2t0r:4i8c2-
|
2012
; i Ei RE2 8s gs
i i ol
il1 = 1h ijil 3 i |
PonsCl |
ql
IH
0 a | ma : 88f8
if 1 - . .a!i1k8i3
1
C202
ProectNoe 454.100
B1sASE1 Ne oe oa
00er id
44 ia
ee maa1 '
1
''
ITHEEEEen a i
0 ereeazn!a :'
PI ASE TS Sx 0 iu a a ee aay' '
PI ES STORS 00 00 0 a R. e me ' ''
oa
''
Poirei=t az zmogs ssn keogs i3ld
n2043
# 5 = a. ZK LE] "am "oss RA B38 ~8
ni
1
"
noi=a
ne
vy
"a
se 0
1
1m
1
n
n=
ms
ae
a
&*
n
nro
un
n
LI
or
"
1
n
noi=n
-
a
a
an I
1
Ta
'
1
n=
Eh
ne
"
gC
n
To
1}
I
LENE |
-
~1
1
oa
-
.". Fa
t. 0
[}
nm
I
1
EE
.
C20
Coos
C206
C207
---
C2058
C2519
C2050
36. RWoiblldilinfelIontaerdnastional,TLrtdi.
ProjctsNuimbtere45t4-104di
ApTpeenzd] CartioffAincstyes
cn a Centre.AnalyticalLaboratories. Inc. CoINTEtRIrM CLERnaTsGIOs AFROeIFes ACmNAA:LYT8SIEAS Dott Ai 310 [---- [RI ------ Xesme D316 Fe i oa oem -- evo SOR
ToStyS tom O = 30 TP -- Dh PuritybyDSCi generallyno applicablefomaterialsoflowpurty.Noendotherrwas fr ie I T ed-- m t-- iihe g-- s L-- C -- ee, Bt oNTnmh H Nioeenesdy, Tn Tesi helobd 0ptfrm CESKOOH) vkvoxtce EPAGodbyPreSt(0CF 140
F----
ier
n2051
-_-- 3.
YvWiulddllijfee iInntteerrnnaattiioonnaall,,LLtedd..= 0 0
PPeroijeectNNeubmbeesr 4t5i4-s10n4
ApFpoeensdix1 CerinofAnalysis
INTERIM
a CERTIFICATE
Cr OFANALYSIS
ContrAntti LiberaCtOeA Reermes G23018A
Lotnypoeste
i s --esee ---- a ------i5---- ---- Cr----a------
be oc,t ry To ifeEa ue a wsCC dpC6 nti dohmtsc,h
voit, ly 2 SE iE Lin
ler
everyG( p L ule]N.lb
anon
sus
0
C2052
38. LWiIldYlifEe nIontrenranattoinonaall,,LLdtd..
PPrrojiecstNNeumbbwere45t4i-1o04r
Dict ndSupApplepFeonduil1xsions Wildife InternationaTl,abLild1.e Game BirdRation!
INGREDIENTS FieCom Meat S`PShroeyoBunevBauniMseesal,48% Protein `DGANrgrewladdWyShLpeeeiychioal0n,6s0P%Porottein ENVxieatonntCnduPMrteisins +rLiundeebelow) SOaTLokulFoedmzeFdemme)
PERCENT 0h) us 3i6065w50 G4S250o%00o a0a3si iTo0om
VpinpmidntaoedEMlivoinnPrenis,ewhre ited 032%ofsin, nous SepPpeToon:
VVRiiaaatninnaD, Nc
2To000000001001;0. rr
FVPoiaeannhaeiBdce Acid
wTogt woSnpgr
PBTorhtiosnne e Vining
1S1i32rpear
NZViiinanenginK(MendiosDimtypyitidoc isi)
201000251p3g8o,masn
iCoonmtaer
mS1Spgeanne
So*nTahmutdausis fo
inof 27
protein
iimof 2.5
rPries retsd mas of5cde
* Femotion ByProductsSuesofseni Groves Fc.
n2053
3. Wildlife Intaernnatiaonall, Leted,,
P Projete Numbei r 456-1s 04
Ditand SpApppeemnteinxF1ormlatons
Vinsad lTicezs Conn
_-- _--
TWermseea pov
0000
OARANTEEDANALYSIS
VVViiinensnaoan HsSoionm ac
2PTa0ro1tTmoo0ono0 i]
o"sBooemrmloo,ircyy oSontis
NViiwmeon12 Fonda
o1i23%s 100
iporfnohste "oti
m aTPenornisceHtytade a f275o5 m 50j0Lo2oonstie
soewg
VYIiionlmRmiEmnDi)aIn.eESStNpK,TdlSSea:meieiCn-pliAprcoluterCs,dlAouifiVmtniCaaSoePrrFGoooelbgoeArf,ce,VNTisosmiSi)p,iipOta.vTV ioobeye Br A8 cth Geeof
ion toe.
po ie brid,
|
ThnJesGlaoinpRptodcrceDooURtpse:ai| pvlostpFeope iofposioly 2 gramsof at isin nd
|
2054
_--_-- "a-
Wildlife International, Ltd.
Project Number 454-104
DAipcpePrnpdairxuIiloln
19,20P0r0.enNionmeinfaolrpPFrOeSwpearwrparase3ptsarfieodloolonnwsF:ebruary 28, 2000; Apel7, 2000;May12, 2000;andJune Control Noemi reuied.
Lsppmais G2pmai: 6pmaii
03084 g PROS +60g9ri9n LOT gPROS +GISRD gration 10839 PROS +609i9on
rnsBasaltoraoWnarwinag webilgenhdeedr.nToheacsetdsuHbotbaarctsmwiesnwgebigvh.ed nAop8 predrweoi1gi0hbogzaotafarntidolsnwnyals a`mdliodnereitandagrdwobeatoshnwtetab,khlTeenwnhtdeeeiorgcl.rheTubashodhseeatrbwnlwayeasaosrdreerairsicsoeofendciowronitselsohlwiiuednnraceteoisrnowtinia.emtTihdnhaaepttpiteroedonsrfxtisiomuoambtsnettlhaoyencasenwteiamnesgtbirobawoneslwf,leworwrideitdtlohsthethreeaWeeadrbfbienietgnhgge esphroutsoimtmb1eea5boimwdiclm.lesTy.heThbeopwrewas wplaascweedigohne8dinHtooba1rt0m0i00ne,a4l0dsG,eplcaocendeiswapepreoprmiaotteely
labeled freezerbags, reweighed, and storedfrozen.
Asode,teappropri prixwas incorporate toefal itsfollows, Oppmais 2375+k12g50kmgltimeostonne Lsppmai 1000 Premix +275 kg tion + 125 kglimestone S2pmai: 1000 Prem + 275 gration + 1250kg mesons 16ppmais 1000 premix+2275 gation + 1250kg esione hediswermixed oppositely20 iusin PattersonKellyTrinShellleer
C2055
_--m--m---- La.
Yi Wiildld ife Io nterk natioo nal, Jm Letdd.,a 0 ionFPra rojclt Nsul mbmere454n, -10m4 Appendix Iv
"The Analysisof FOS in Avian Diet
C2056
a. LaWilgdleife IInntteerrnnaattiioonnaall,,lLtdd.. 0
PProjeect Neumbwer 44e-10e4
ArpENDIX IY
ANALYTICAL METHODS AND RESULTS
Tae
--_-- TypicalLC/MS OperationalParameters
INSTRUMENT:
HPF oerkviner lEeaimcreori TnSbCMoTolEdo XoeAp1Pr10y01fT0osi0gLrEh MePe.sfS rOmapcep eeLdigisesdsnoioprpcpeoesdmois nmttsogwih
mirage (S.
ANALYTICAL COLUMN: Keone Bea Coscoum (50mm x2 mn LD, imprice ie) OVEN TEMPERATURE: 30C
stop TE:
500 mines
FLOW RATE:
0220 mLrnine
MOBILE PHASE:
IUECTION VOLUME:
72.0% Methanol : 28.0% NANOpure Water containing 0.1%
sop
FormicAcid
REpTrEosNTIONTIME: Jr----
|
REITNETENRTNIAOLNSTTIAVNEDARD Avpeosimatly 26 mines
!
MrOoNsITORED MASS: 49860
= IMNOTNEIRTNOARLESDTMAANSDSA:RD. 4267 ams
"
n2057
a. LWy ildle ifeIImneterrnnaattiioonnaal,,LLtdd.,
PPreojeNctaNubmbeesr 4t54i-104
AmrENDIXY Taste caLcuLaTions
TheconentraonofPROS foundatth instrumentwasdetermined sin heflowingequation:
PROS(ng ten =EL
I) sr again
-
Theconcentration, equation:
xprssd
8ppm.fox
cachsample
was
detmined
sing
he
following
R05 grin te FOB
tt de int
`DeteofrLimmit i of Qnuanatitt atioin (oLOn O) `ThemethodLOQ,expressedasppm a.i,wasdeterminedusingthefollowingequation:
LO ppm. owessandrconcetaion (mg 1) x over ditionfcorof atxbak
"over tionfico ofabnspe-252 lameLcGiocr
.} ("f Thepver lm, p.mi m.e.s.sTehdi nrcataicohtsimps1e00iivtihdepdebryttherescoomviernylcofotnhesmtertahiodn oatf ehxtchesvaelmpolfe
fortification.
%Recovery r=BEEAL mesnidsample
n2058
-_--
kg
2
8
g
|}
i
i'
`2%
233
mss
zs
i
I
Pi
li
mi | NEE Hh{
J |||3
i sees eon sooo YYY : [
330
i |fo
I
da
a4ll
ll)
RfBfiEnl
:i
HEEP ER EEOR ,Fer
1
lil
|
-_--
oH] ; uo.
Colle :
5
jj DEC
o. Lf
wow :
SC
}
ji fi | Ei E asied guE ssd aun E5
I E-- 3
4
NR
gh
|
. $
Ii
|
i al. e o 2
nl
Ih
sl
--WildliPfCe ITnTtEeTrAnTaItioonnaanl,,TLtdd.
PPeroejeNcteNmumbbeedr s45t4i-1o04s
Cmbcommiti)a!
APEDIXIV Taes Vertcadonof PFOSCancatriatAviaonnDist oIoDNmdimcba Gltelr CVoTowmomemits70e5 csoumVneu=tnaen,eN"moFter
o
3\
\i p<ordm
x
}
o
.:
<atda
wa
B
in
se
'
-
(tien
"
"8P
`:.
ii1a0gt0 Pixpsroimatetl m
@
se
:
,
Ce niien
"
o"%
`. pPro xl-esoepass
ed
wom
"
ve 120 '
.
(ite
wo
23.
`.:
ihwrs fx=NiiolEtea us
iC1o1mo2n0ne1nbNoeirsioceoeaocrttopcreonecdeemftrohonosgfoeidpiotehnhe0s0 tstytG3o7tsp3 tyoo1G .3 14pFee ac
moreending
"0061
a.
T o
[oI re
IES i
to
oases c= x a yg
Ho
roe
Ne '
1
[I
'
[a
'
efa
tsa oa sar!
[|a = ''
'
[ON
TTETE
"2
oe \
i
50 3 eal
j253y
}
C2062
_--_--mmm--m--
-ZAWilYdliefe IInntteerrnnaattiioonnaall,, LLetdd..
PPrrioctvNmubmbeears4e54i-1o0s4
APPENDIXV
METHOD OUTLINEFORTHEANALYSISOF PFOSIN AVIANDIET Preparematiforifaionsupe nthedesi avinfdstock inthedey i chine
Wei
gh
10g
sa
mp
les
fh
e
mats
bak,mare
i
frist
and cstamples ioweighboutsand
trantsof8-e0zr. Frenchsquareglassbottles.Recordweights.
Forcachsuaple,measure1007mLofmethanpolwrit+vaigradu lind snd raneinotheFrench
1
Copbodes 0dpac onshaker blep.Alploowtshiamm2pal0ecspeys.ake for iuof 30minis
Vaca
ierwithqaliaiv Gi
1
pepe ndrinerendfc 3 msswith methnatonloel,
`Traethefile to
1
200mL. volumetricfa
ndbrig volumewithmethanol
,
'
|
cimebdont UsemOapnyet lteedios, uioe1F5o oPFtO oGce Prepareappropriatedilution(s)tobring finalconcentrationintothecalibration rangeoftheLCMS
!
Fire T- ly
4 `Ampulateandsubmitsamplefor LCMSanalysis.
med Tow Sh ForG5 ly ofFFOS i Gi
C2083
a. -- L Wildlife International, Ltd, e PPreojlecettNuNmmbebre45s4t-1i0n4
APPENDIXV
120
Iw
0s oa ! Ii
2 :
02
|
0000% 00s
re
on om
02 0% 0% as
os
|
L--
Concentration(Ratio)
|
Figure 2. Tisywpeiicgahltceadlib(r1a5t)ioncurve for PROS. Sl=o248p67e9, nerept = 007396; 1 = 09985, Carve
tere --
--
2064
Cn2065
Cronat
C2087
C2658
ro069
--
cr2u70
-
Cnoo71
roo
_--------
OOOO
8 E mo 23 FZ "esa REBEE # TE FE =. aE &
=
FI
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EPL PEER PATHOLOGY(BOREOR
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WEOrSLRFoEOyIToNTvEeReNAnsTIsONidAL:oLTsD orTENT nT
PATHOLOGY SUMMARY
Lohmkroscopcxamiatonvas pada acosoelected h(tiPsocsrueuswofraroroomncaadanulsttsmtdaolnaecrawcnhdic,fhepmraaleescNsioreathvseearlntrb0sob3cwhoi)ntean(nCaohltionfunstovfifreogrinhifeanfuosa)1r5il wSheeeykasc. u0SbeolvewcatteitdeetvieshsrousefsaefkcrtomsauonmtinrneeastcedbyapbgprohoxtiimssastseClcoyorp12sn-.swTeseoke-ogaldbnoffisporofienfg soifs epetrairmyeentxsp!odseesifgnawfaesstsblsoavnceeovert sat soeprc Toe
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Laboratories, Inc. (EPL), where they were embedded in paraffin and made into
of`hovelamoawttioanxg:ylsiunuaernsdgfelotsaiinaa-ddsutdla,tisnpendrdseopctvinoinssgoonwgKaliarsesrs`,rmaiscmrnion,sdcoGpooersgldihde(soi. rcyTrheoctyr3,0 rss ofFace, nddpe ase. Insomecase.noprtocsrons
povaeleg, and icoscop fing were erie tissues were sectioned along with adjacent protocol-requ rable ired tissues; these were IncidncAetTabtlens. rMocurtoesdcobiycpirntgsoerspaecrstniahriaHmiidftomotne Stites eed nhHispaihioy incre Tans, Morosoares
`were graded one tofive depending on severity. Nongradable findings.are listed
as present (P) andtissueswithextensive autolysisarelistedas (A). Al findings
1
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Project Number 454-104
Wheart 4 uyte510 ameactadl,fom theros Nosy ardrovdd byWi
Als BESULTS and fing ive th arfte esc sy
intervals. No effects considered possibly related to test article (PFOS)
rss of Fabrics, provntic, `administration were noted in liver, and ovary af ml and kidney, adipose tissue, ore st gallbladder, pants brain,
r1ore7ths.ee6icr5oTe3ffooasftpr.ouinmomgfr,s,oolrscissn ftmheaamttaeeslmtsessopeeifxmohafbftrsoipzeroiansgpmoammolaeascs.rseslmnydeirdsoccohsaerasmsciioedr.aaadrobnay heo (17h6ugohmspae.r.mattsoeg.enreseisiwgsnsiovaevndm)asteaerteo ohesthercsormnepeanttt to rosotsyoncshistoavnedwi1.t2a5raym phtostse.tTphsrtoecsteesfesnoerr we S00h1l7o.k6a5l ha1a.rdcemavnorn,pThmeyovcstabneenoafoohnestseertsarenmnry
edo Tote of al fring wae eo a the small group sizes, but the possibilityof
a test article effect cannot be entirely
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inflammation`ofadiposetissuewerecharacterizedby infitratesconsisting
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SA. crodaoct.reSaaatoawnachcst anosffcoarianl arStosldiankebarsotphi.l tal daratconncionmseseireestsse eintegshe sro vatyn. dor,raends eli, ar nt apnetne. Cranston tame
characterized by small mononuclear cells, macrophages, and occasional
multinucleated giant cells was associated with foci of foreign material (deep
c`emoaamgmelnotnatiglrnobcu0al1ecs0oaKnt)d.obnriCgu hot plinaka acmoprpshaaouis mgatoehriyarlesconsepiemsrtaeatnntotnweitt'hih ha own oongnamtepgoomnrtanocitosopsrsia kaopcooreyrrooagr,, d Kurcto s, anresm yvpreoe nesaist;es atotthe,ySfathogmimutweert partici ductwer tad av accorfro anton
was eto nore 55m i fsa. Ta ofnadlpe omad 1.8 ppm a.i. offspring female. A small focusofheterophils (acute inflammation)
, female exhibited amyloid deposition associated with secondary hepatoceliuiar
hypertrophy and necrosis. Liver amyloid deposits consisted of amorphous, pale
`bluish-violet matertal which filled and distended the hepatic sinusoids, and
cone be `compressed adjacent srs hepatic nud cords. hep
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vacuolization. Fattychangedenotedvariablysized,sharplydemarcated, clear
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