Document penZj12zZvj8zneoRdx0v83kj

Toxicological Summary PFOS Dietary Chronic Pilot Reproductive Study: Northern Bobwhite Test Substance: Perfluorooctanesulfonate (PFOS) Structure: h1e-pOtcatdaenceasfullufoornoi-cpaocliads,si1,u1m,2s,al2t,.3,C3,A4S.4H,52.57,96,56-,37,973.8,8,8- Test Remarks: METHODS Twhaes steosrtesdubusntdaenrceamibsieantwhciotnediptoiwodnseran(d3pMurLiotyt w#as217d)e.terTmhienedsatmoplbee 86.9% by LC/MS, "H-NMR, and elemental analysis techniques. Method: ASTM Standard E1062-86 and FIFRA Subdivision E., Section 71-4. "Type: Dictary Reproduction Year: 2000 in life phase, 2000-2001 analytical phase, 2003 for final report. Species: Northern Bobwhite (Colinus virginianus) nEoxmpienrailmecnotnaclentdreastiigonn:s oAfdu1l8t, 6b.o2b,wohrit1e7.q6upaplm,wewreet weexipgohstedfotoa PpeFrOiSod ionf stihxe wdiecetksa.t Concurently, a control group was mainaincd on anomtrated feed. After six weeks of teoxpgorsousrse,neqcuraoiplsyf.romTthhee c1o.n8taronld a6.n2d p1p7.m6tprepamtmternetatgmreonutpsgrwoeurpeseWutehraenimzaeidntaanidnesdubojnecttehed nreescpreocptsiiveede(xTpaobsluere1.regEifmfeesctusntoinl aWdeueltkh2e0a,ltah, wbhoidcyhwteiimgehtthaenydalfseoedwecroencsuutmhpainioznedwearned eevvaalluuaattieodnwoefektlhye.daBteacawuassedtohneetiwnotlwooweserpadroasteesawnealryesetse.rmTinhaetefdisatt asniaxlywseieskesv,alstuaattiestdicaalll deaxtaamicnoeldledcatetda furpo.mtthhreoucognhtrWoleeankd 617.f6orppamll twrecaattmmeennttgrgoruouppss.fromTWheeekssec6on0d 2a0nalysis Aphdouolsttiqmuuallatiwoenr.e bCroonusgehqtueninttloy,reipnriotidautcitoinveofpehgagselapyriinogr bteogatnhedounrsientg othfetfhierststwuedeykboyf dPoRsOiSn.g. AEgessubsleatidofdeugrginsg caolldcaciyedpdeurriiondgsWteareiknsg 1onantdhe6soefcotnhed tdesatywoefreWeaneakly3zewdefroer econldlpeocitnetds,, iinnccluubdaitnegd,cganpdrodruecptrioodnu,ctievmeb.ryeondvpioabiinltisty,wheartcehabeivlailtuya,tehde.alth, Raenpdrosduruvcitviavle, awpeprreoxeivmaaltuealteyd1i2nwteheisksc.gNeccohroorpts.icsOfwfesrperipnegrfwoerrmeedmaoinntoafifnsepdriongn fnormo-mrecaatcehdtrfeeaetdmefnotr lairvoeurp,. braLiinv,erkiwdeniegyh,tsgownaedr,e prreocvoerndterdicuflorus,allganlelcrbolpasddiecrd,quaadiil.posIen taidsdsiutei,ona,ndsaBmuprlseas ooff Feaxbarmiicniautsions,amples were collected from adult and offspring. for histopathological 1 600008 hAtet1he8eannddo6f.2Wepepkm6r,cbaltomoedntsagmrpoluepsswaenrde wceorlleechteednfcrtobmanalilzesudrvainvdinngecardouplsticbdi.rdsAtfrtohme ime of necropsy. livers wre also callected for chemical analysis. Blood samples were acalussloohaccnooallsllieeaccttaeednddffnlreoocmraodapudsluylttabtibritdrhsdefsobinechithenemigccoanlotfraomWlleyaesnskd. 201.7P.i6 Aopt p0nmescrwtorhpeasanyta,mseinIat eogrsofhauemppsolTepsrsiporwreirgteo, aAbnldaodloiydisiosnsaalmyAp,llletsbislsowuoeedsrecforlolcmeocltl1ee0dctoefdfrfosmpfrriaondmgulwe1a0r.nedocfooflpplreiicntnge.dg fwfriaoosmm. sceacpcaahcrha.ttreedtartiematetnomtesngetrrouugmproaufnpo.dr hemacytepltlet fractions. "NToarbtlheer1n. baEbxupwehriitmeesn.tal design fo the dietary chronic pilot reproductive study with om (Pvereido)d CoTmser Ey Halth body weigh, feed consumpiion, gross h2o 6 mproordpuhcotlioong,y camnbdryio.sopviaahbiollitoyg,yhaotfchbaobdiylyo,rgaonps.inoegs jo health and survival, PROS concemrations 1 er egg omfefmpbrriannge,quayiollsk, albumin, snd blood Serum of adult and Test Bird Age: Adult qui] (approximately 30 weeks old a exposure nttion) Number of Replicates: Five replicates (pens) per treatment group Quail per Replicate: One male and female per pen bsFatesueidsdy, oaondbnoatWchwaetaeeedkru.:lt Fqouoaidl aanndd owfaptreirnwge,reFpereodvicdoendsuamdptliiobnitwumisdumreiansguraleldpohnas8speorfptehne dAentaelrymtiicnaeld bMyonrietvoerrsien-gp:haPseFOHSPLcConacnednrmaatsisonsspeinctfreodm,ryl.iver and scrum samples were mpSteuarltfiiopsrtlimeceacdlomMtpeoatrheivosadolsun:atpUerpoocsiengdncuiorfmeicpawlnaettsidouinsfefodefrttehoneccetsoemstpb,aeartnweeahennaelyrasieesatoamfemvnatricgafrneocuecpes(.AwiNtOhDVucAnon)netrwtoa'lss oTnhley sthteudceonnttrsolFaensdtthwas17u.s6epdp1m0 tmraekatemesnittigsriocuaplscwoemrpearciosmopnasreidn.those msances where Average Daily Intake (ADI) of PFOS foreach ratment group was estimated using data 2 0007 from cach pen in that treatment group, without taking into account potential differences between the male and female paired within a pen. Food consumption and adult quail body weight data were averaged over the duration of the study and the ADI was calculated as follows: ADI(mgfkgiday = AXvCeTrEaEgAFevEeeScrCdagCCeoOBnNsoudSmyIptwDieoin(t(g(eRgsEdhbCdi)UndEEd0a0y)), pone pm) (Equation) `Test Diet Preparation: Test diets were prepared by mixing PFOS into a premix that was. used for weekly preparation of the final dict, RESULTS Measured Diet Concentrations: Chemical analyses were conducted by Wildlife Intemational Lid. to confirm PFOS concentrations in the diets. The limit of quantitation (LOQ) was equivalent to 0.879 ppm, wet weight during Week 1 of the study, while for Week 6 the LOQ was 141 ppm. The percent recoveries of matrix spikes (averaged across replicate samples) from the test diets ranged from 97.0 to 113%. Analyses of control diets showed no presence of the test substance or other co-luting compounds. `Samples collected for verificationofdiet PFOS concentrations from the treatment groups had measured values that ranged from 95 to 111% of the nominal PFOS concentrations. `The mean measured PFOS concentrations for diets used in the study were: <LOQ, 1.8,6.0 and 17.6 ppmin feed Analysis of diet samples collected from Eeders aftr being held at ambient temperatures for 7 days averaged 100%, 103% and 98%of the Week 1, Day 0 values for the 1.8, 6.2 and 17.6 ppm diet reatments, respectively. Mortalities and Clinical Observations: No adult mortalities occurred in any treatment `group during the study. While several quail were noted with head or foot lesions and feather loss, these signs were considered to be the result of pen wear and/or pen-mate aggression that occurred during the course of the test. All other quail were found to be normal in appearance and behavior. Adult Body Weight: At both 6 and 20 weeks, no adverse effects on body weight were observed in females from any of the treatment groups when compared to controls (Table 2). In males, there was a statistically significant (p < 0.05) reduction in body weight in the 17.6 ppm treatment group at Week 6 and at Week 20 (8.8% reduction from controls). It was concluded that this reduction in body weight was treatment.related. For adult 6m.a2leps,pma PNFoOOSbsaenrdvatbheleLoAwdevsertsOebsEfefrevcatblCeonAcdevnetrrasteioEnff(ecNtOCAoEncCe)ntwraatsiodnet(eLrmOiAnEedC)towbaes determined to be 17.6 ppm PFOS. The NOAEC for females was determined to be greater than or equal to 17.6 ppm PFOS. 3 GEOGOs F1.e8edanCdon6s.2umppptmiotnr:eaNtmoenttregartomuepns.raetlaWteeed keff6e.ctsWhoinlefotohderceonwseurmeptsitaotnistwiecralelynostiegndifiincatnhte reductions in food consumption at these exposures, these reductions were not considered treatment-related due 1 the lack ofa dose-response relationship. The significance of the arpelpaatrievnetly rleadrugcetiionncreataseWeinekfee6d actontsheusmepttiwoon dionstehse mcoanytrohlavgerobuepenat athcisontsiemqeuienntceervoalfaas compared to that abserved at Weeks 5o 7. Thus, the feed consumption ate in all PFOS treatment groups was significantly reduced from thal in the control group at Week 6. Mfeoerdeocvoenrs,umfpeteidocnonrsatuemsptmieoansurraeed iant WPeFeOkS StrfeoartmtehnetsgarmoeuptsreaattmWeenetkgr6ouwpes.re Tghreerateerwatshana cboonbswihsitetnet qaunadilstiantitshtiecal1l7y.6sipgnpimficPanFtO(Sp t<re0a.t0m5e)ntreadsucctoiomnpairnedfeetdo cqounaislumipntithoen caomnotnrgol. rcoenaturmoelntanadt W17e.e6kpsp2m,3w,ea4i,m6e,nt8s, aantdWe15.ek T2h0e waevreerag2e88feegd fceoendsbuimrpdt-idoany raantdes2i4n.4the NfeOedAbEirCd-adnady, LrOesApeEcCtivfeolry.feeBdacseodnsounmpriesounltswafsro6m.2thaendWe1e7.k6 6 statistical analysis, the ppm, respectively (Table 2). "Table 2. Average daily exposure, feed consumption, body and liver weightof adult quail exposed 10 PFOS in the diet. * TrGeamtm)ent (mgAkDgLbody (F@Cfeed Sex Body Weight (9) Week6 Week20 Liver weight weightdsy) _/bird/day Conrol <LOQ 282 M2357 F241 2201268%271 736440522210240456 18 0174 0x2 M2244 35010514 0007 Fo2lit36 6323+1.668 62 0579+ 23vx3 M 207211 0.066 Fons: 53.6682434+00.658065 176 200% 0249 2342 M1906 1970%7 2527+0359 F_231414 20:33 685141880 sCaocrrilficnedd1W77e6epk6o.m wAlelsamecnstsrweegiSveCnTRmeRaAdnsWsendekst2a0ndWaHrTdedeviat1io8ns3.nd 6p resin were cAovnesruamgperiDoainlythIonugtheW(eAeDIk,6mogrPtFeO1S.3K4nbdod6y.2wpeiogmhtgrporupsdaa)ndotfhProFuOgSh Wveacskba2s0edforanthaevceornstgrsolosondd F1oo7dpcomnsgurmoputpis.on baonsWeeke6 data Asensk indicatiecasl dren from the contol tment at< 0.05 `LWievcekr W6,eiigthwta:sSinnotcepaodsuslitblqeuatiol cformopmatrhee t1h.e8seantdw6o.2trpepatmmetnrteagtrmoeunpss were termined to the controls at at b`Woebewkhi2t0e.quOanillyinthteheco1n7t.r6olpapnmd v1e7a.t6mepnptmhatrdeaatsmteanttisstiwcearlley sstiagtniisftiiccaalnlty reevdaulcutaiteodn.in Mlaivleer wtreeiagthmtenwthgernoucpomwpearreednattoscaotnsttroclasll(Tsaibglneif2i).cantIlnyfdeimfafleeresn,tlfivreormwceoingthrotlss.inThthues,17f.o6r mpaplm.e NquOaiAlEtChe wLaOsAgEreCatebrastehdanoonrdeeqcuraleased17l.i6veprpmwe.ight was 17.6 ppm, while in females, the 4 GOOGOY Gross Pathology: Adult quail in the 1.8 and 6.2 ppm treatments were sacrificed at Week 6, while quail in the control and 17.6 ppm treatments vere sampled at Week 20. All quail were subjected to gross necropsy. All findings were considered to incidental and not related to treatment. Incidenceof small testes was noted during the gross necropsy in the 17.6 ppm treatment group. Ten juvenile offspring (approximately 12 weeks old) from cach treatment group were sampled and subjected to gross necropsy. While feather loss was observed in juvenile quail, this loss was considered to be incidental and unrelated to treatment since the frequency of loss was equivalent across treatment levels. No other findings were considered to be treatment related in the offspring. Histopathology: There were no lesions in the liver, kidney, proventriculus, gall bladder, ovary, brain, or bursa fabricius in quail offspring at any of the PFOS concentrations tested or in adult birds collected from the control and 17.6 ppm treatments. In addition, there were no treatment-related lesions observed in the adipose tissues or testes of adult or juvenile bobwhites. However, there was an increase in the incidence of small testes size in males from the 17.6 ppm treatment, and this was correlated with a decrease in seminiferous reproductive tubule diameter. phase regression, Whilea a normal decrease in testes size is consistent with physiological phenomenon, the frequency postof the incidence of decreased testes size was greatest in the 17.6 ppm treatment. However, since there was a small sample size of birds at this treatment (N=5), the significance of this observations relative to PFOS-related effects can not be determined at this time. Spermatogenesis did not appear to be adversely affected in the 17.6 ppm treatment when compared to control males. Reproductive Results: There were no PFOS-related effects on bobwhite quail egg production when compared to the control group. In addition, there were no PFOS-related effects on embryo viability, hatchability, hatchling health, and survivability. While there was a statistically significant (p < 0.05) reduction in the number of viable embryos in the 1.8 ppm treatment, this was a consequence of fewer cggs being st for this treatment group. When reproductive endpoints were expressed as percentages, no statistically significant differences were observed among any of the PFOS treatments when compared to controls. While there was a slight reduction in embryo viability among quails exposed 10 6.2 ppm PFOS, this effect was not considered dose related sincea reduction in viability was not observed in the 17.6 ppm treatment. Hatchling and Offspring Results: In comparison to controls, there were no PFOS treatment-related effects on the body weight of cither hatchling or juvenile bobwhite quail (Table 3). In addition, there were no effects on juvenile liver weight for any PFOS treatments when compared to control liver values. Based on these results, the NOAEC for offspring endpoints was greater than or equal to 17.6 ppm. 5s CROGLO Table 3. Mean liver and body weightof offspring bobwhite quail. * Treatment Corral HatchlinBgody Weight (g)Juvenile 61205 T7510 JuWveeniiglhetL(igv)er TTH60339 6128ppppmm 5s76220065 117759515 43978556+01435637 TAT 17p6repsepnm mm a6n0:0d5ear 17328 342930495 "Body and liver weightsforjuveniles were measured approximately12weeks posthatch. Blood and Liver PFOS Analysis: "RePsFuOltSsCoonfcetnhteracthieomniscianlAdaunlaltyssis of blood and liver showed that the adult quail accumulated PFOS in a dose dependent manner (Table 4). Table 4. Summary of analytical results fo adult quail liver and serum samples collected d auEaa pmeoe neCom during the pilot PFOS reproduction study. * Sem Con C(opnptmro)l wEegek) SeMx OTS(g0/e0) 9 F <L0Q 71(.6u3g3/05m43l)4198 150 6 MFoo 001302302206088 31783744410007 620 6 MFo 10.91e4400106s 73723244107937 176 2 MFo 213%60+s210601 419594+578.s3 Tereainghtrbs.CTheoLOGoforThEtRwEas0.e1ipnig andefonSdeudewaasns001 Ae re epee or Lrfiravoegmrehadnedcrseoorunnmascnroencacemrnetsnrtaatihmoandesnvw.eareiBaobvhelrosawgeepdhaefloaspcaachtchererplaiociatteoosfafamoplmerir(n-ts2)ala(neLd0sGt)hheonmTsaoFmopalaebls wgeirsee Cmalctrrmaasniadnmspsttlaaenncddawarerrdde,de0ve.i0pa0ot0ni1oeundsmfholrovi)eswiaEserLup0sGd1fhorvslos00p0o0r0e0s'<(oLnOeG.RfAoll eaieaoeesofsahmeplleoswaesnt cAonnacleyntitcraaltiroenssulbtestswheoewnemdaltehatantdherfeemwaales baobmwahrikteed qduiaiflf.ereInncemainlelsi,veirvaenrdcosnecreunmtraPtFiOoSn.s wrceartemetnytpicgarloluyps2. toTh9e4 staimmees wgarseatoebrsetrhvaend tfhoart soebrsuemrvecdoncienntfreamtailoenss owifthPiRnOSt.he sMaamlee bcoonbcwenhtirtatqiuoanisl ohbasdersveerduminPFfOeSmacloenscesnatmraptlieodnsftihoamt wtehree 4sam1e0.24trteiamtemsengtregartoeurp.thanTthhee rierleaattimonesnhigprobupest.weeSnerfuivmertoafnidverserartuimos croanncgeendtrfartoimon0s.1w1asto v2a6r2iaibnlemaalnds dainfdfefrreodma3c.r2ostso 6 600621 b1o5b0whiintefeqmuaaliels.wereT:he serum to liver relationships for PFOS concentrations in adult Mak: Female: Serum PFOS (ug/ml) = 91.638 (Liver PFOS, ug/~g) 4.8447 Serum PFOS (ng/mL) = 15.653 (Liver PFOS, pkg) + 5.5355 R= 0.5997 R= 0.3290 a"Tnhde saenarluymtiPcaFlOdSatcaonaclesnotriantdiicoantsebdutthatthathtehreesewadsataa wdeorsee dseexpesnpdeecnifticinacnrdeahsieghilnybvoatrhialbilvee.r eTsotiemaxtaemdinaevertahegerdealialtyioinnsthaikpe b(eAtDwIe)enofPPFFOOSS ebxypoadsuulrtequaanidl wtiasssuceocmopnacreentdrattoiloinvse,r atnhed bseecrauumsecotnhciesnptarrataimoentsercoislluescetdedasfraommeeaascuhreotrfeaetxmpenots.ureAnthaAtDaIdulwtasbirudssedmaiyn ethnicsoaunnatleyrsiisn cthoencceanvtirraotnimoennsta.ndThAeDIreasrueltgsoivfetnhbeelroewg:ression analysis for both serum and liver PFOS Male: LSievreurmPPORSOS((ugg//g)mL=)1.=269042.9(4A8D(IA,DmIg,/mkgg/bkogdbyowdeyiwghetiigdhatyi)da+y)03+191.8344 R= 0.8964 R'=03233 Female: LSievreurmPPFFOOSS((gu/gg/)m=l)0.=712439.1(4A8DI(,ADmI,pkmgg/bkogdbyowdeyigwhetiigdahyt)d-a0y-0)9235198 R= 0.4080 R= 03871 tThhaetsmealreegsreascsciuomnulmaotdeedlsgraeraeterincoangcreenetmreanttionwsitohftPhFeOsSeriunm:botlhivetrheraltiivoerdaantda bthlaotodshsoewreudm PinFcOoSmpbayrimsaolnes10afnedmafleemabloebswhiis luenkqunaoilw.n,Haonwdevtehre, itmhpeacttimoef-dcegpgenpdreondtucatcicounmuolnatfieomnaloef asdedriuimonaanld plihvaerrmPacFoOkSinceotniccentdartaat,ionas cmoourled naoctcubreatdeeteevramliunaetdiofnroomfthPiFs OstSudyu.ptaWkiethaonudt ldievpeurrPatFiOonSccoounlcdenntortatbieoncsonsdhuocutleddbeatetvhailsutaitmee.d oOnvaersaelxl-,spitecwiafisccboasnicsl.uded that serum and CPhFeOmiScaClonacneanltyrsaetsioonfs1i2n-Jwueveekniolledsjuvenile quail serum and liver samples indicate that 1P0FPOFSOcSonpcoesntt-rhaatticohn,srpaetrhseirstthienirjusveerniulmeaqnudailli(veTrabPlFeO5)S. cJounvceennitlreatqiuoanisl wweerree ennottireexlpyodsueed tthoatcownhtiricbhutrieomnasininetdo itnhetheegjguvferniolmeaqduualitlsfeafmtaelre1q2uawilese.ksTohfegsreocwotnhceanntdradteipounrsatrieonp.resTehnet concentrations are not representative of that found in quail immediately post-hatch, 7 6000T `Table 5. Concentrations juvenile quail of PFOS in the liver and serum of 12-week old Treatment Tver Cone Serum Cone. TT C(opmpma)l <(LHOeeQ)" --<(rugo/mQl) 6128 <L0Q 00182400156 00324200198 0203+0.0838 RT oncen1t7at6ion re presented0.as0m5a9n3s+w0i.h04s1a7ndard deviatio0n4s 1D3a+t0s.1ge1e4n0ons TwheewLeOigGhtorbasliisv.er was 0.01 g/g and for seum it was 0.01 g/ml cToonceanstsreastsiontsheanqduaengtgityatoilvke cornecleanttiroantsihoinps,baetrweegernessjiuovnenainlaelysliisvewrasancdondsuecrteudm. PTFhOiSs abnuatlylseisss itnhcalnudtehdealLlOtQh,e dfatrao,micnaclcuhdiPngFOdSatatrtehaattmweants garboouvpe. thTehleimirteloaftidoentsehcitpiobne(tLweOeDn) average egg yolk and juvenile serum and liver PFOS concentrations are: SLievreurmPPFFOOSS(u(ugg//gm=i))0.=0001.0(0E7gg(Eygolyko,lkp,gp//mlm-))0.000.403064 RR== 00..99800627 "Ths, juvenile concentrations. liver and serum PFOS concentrations are highly correlated with egg yolk tJhuevengrielaetelsitvercoanncdensterrautmionPsFObeSincogncmeenatsruarteiodnsininqcuraeialsefdroimn atdhoes1e7.d6eppepnmdendtietmatnrneaetrmewnit.th "The serum to liver relationships was: Serum POS (ng/mL) = 4.353 (liver PFOS, ug/g) + 0.0720 R= 0.6041 Serum and liver levels juvenile bobwhite quail in juveniles thus are comelated. The serum was 28 + 36.2 and ranged from 0.77 to 147. to liver ratio The range of for the Juvenile ofsex. serum: liver ratio was within the ranges observed for the adult quail, independent Egg component analysis: production from the control, Eggs were sampled during the first and 6.2 and 17.6 ppm treatments (Table 6). sixth weck of cgg 8 600623 s`Taambpllee6d.dMuerainngcotnhceepnitlroattrieopnrso(dwuicttihvestsatnuddayr.d*deviation)of PFOS (ug/m) in quail cggs Treatment DEwgegekMembrwaeneek Iwes`kAlbum6enwesk Yolk lweek Gwesk 6T2owpeplm 0N14o0 0N13o9 0N00e4 0N0od 1N2e6 3N31o 6pm 0N2R5) 0003318) (00001026) (0O00104) (RIST) (S76651) TTConcenratom Tor cas components0r0e8r6e)ported(on00a0w9e)r wER(0B.R0E0.) 13) (651) NNRO nnooxt qepuoracdidulet cosnacmenptlreastbioennsg cobntlamoinhatedi. tofquai (0.0001 gi). cAonpcoesnittriavteiorneloaftioPnFshOiSp winascgogbsceormvpedonebnettsweseanmpPlFeOdSatexWpeoseukrse 1inaanddul6t. heTnhseraendwetrhee wsiignhiftihceanytodlikffhearveinncgesthbeegtrweaetnestthPe FcoOnScecnolnrcaetnitornastoifonPsFaOnSd tmheeasaulrbeudmeinn ehgagvicnogmtphoenelneatss., cWohmepnarPedF,OSnocosnicgennitfriacatnitondsififnercegngcecsomwpeorneenotbssecrovleldctfeodr ocinthWereeaklbu1 maennd oWreemkem6brwaenree PPFFOOSS ccoonncceennttrraattiioonnss.at HWoeweekve6r,whtehnerecowmapsaraepdprtooxiWmeaetkely| caon2c-efntordatiinocnrse.aseThiensethereysuolltks binodtihceatgegtmhaetmbPrFaOnSecaonndceanltbrautmieonnsbyhatdhemoesntd olifkWeleyerkea1c,hbeudtathsaattuPraFtOiSonccoonncceennttrraattiioonns iinn the yolk ofthe egg had not reached sicady state by Week 6. a"TnoaleyxsaemsiwneerethceornedluacttieodnshwiipthbetthweeeWneeakdul6t cexgpoysoulrkedaantda acngdg acdounlctenetxrpatoisounrse, ernedgproesisnitosn p(rTehpearcagtgionmeimnbtrhaene1s7.6weprpemnottrtemvaelnu)a.tedIdnuaeddtioticoonn,tatmheinaaltbiounmeinn dtahteamweamsbrnaotneansaalmypzleed cdgugc 1a0ltbhuemenno.nlinTehaer rqeulaanttiiotrashtiipvebertewleateinonPshFiOpSbdeotsweeeanndacdounlctenftermatailoensPoOf SPFOeSxpoinsutrhee endpoints and egg yolk concentrations for Weak 6 were: YYoollkk ((ngg/mL/)==m6204L1.629()6I(nASDeIr,umm,u/pkge/mbLo)dy+w2e8i.g3h4t2day) + 8.4986 R= 0.7748 R=06637 Yolk (ug/ml) = 5.4416 (In Liver, ng/g) + 49.929 RE =08207 mSionncietorneodPiFnOtSh-irsesltautdey,d qaudavnetritsaetievfefercetlsatwieonrsehiopbssbeertvuedeenfoer gancyonrceepnrtordautcitoinvseapnadraemffeetcetrs in hatchlings orjuveniles were not studied for this project. NCoOrNthCeLrnUSbIobOuNwShi:t quail were exposed to PFOS at nominal ditary concentrations of 0, w1e8e,k6s.2whainlde t1h7e.61.p8pamn.d 6T.2heppcmonttrroelatamednts17w.e6rpepamdmtirneiasttmeernetdsfwoerroenlaydm6iwneiesktse.red for 20 9 6noGLA cNooncternetartamteinotn rteelsatteedd(mToarbtlaeli7t)i.esWhoriloevetrhterseiwgnassoafstloixgihctiteyduwcetrieonobisefreveeddcfoonrsuamnpytPiFoOnSa.t aWteeeskh6adamroentugmequdai1l0 icnontthreol17v.a6lupesp.m PAFCOSWeterkeat6menatn,dbWyeWeekck2020offetehde csotnusdyu,mpmtailoen wbeoibgwhhtistecoqmupaialreidn ttohecon1t7r.o6lsp.pLmivterreawtemiegnthshaindasdtualttismtaiclaelslyfsriogmnitfhieca1n7t.l6y prepdmucterdeabtmoednyt group quail iwnerteheal1s7o.6sigpnpifmicatnrtelaytmreendtucheadsfarnomhceornctarsoeldvailnuceisdeantceWeoefksm2a0l.l Itnestaidsdistiizoen,wmhaelne bcioomlpoagirceadl tsoigcnoinftircolasn.ce oWfhthie itensctriecausleard riengcriedsesnicoen iins @thneo1m7a.6lppphymsitorleoagtimceanltpgrrocoeusps,ovtehre (thNa=t5)o.bseIrnveadddiitniotnh,e tchoentsrioglnigfircoawncecooufldthnisotfibnedienvgaliusadtiefdfidcuule ttoheinstmearlplretsaimnpltheatsinzoe wweeaattmmeenntt--rreellaatteedd oerffmeacltse-wroearteedobesfefercvtesdwearmeoonbgsefrevmeadleonbroebpwrhoidtuectiqounaiilnsthatisasntyudyP.FONoS concentration evaluated in the study. sTthuedrye. weTrheerneowterrceatmneont-erffeelcattsedoenffescutrvsiovanl,anbyodryeprwoediugchttivoerpalirvaemretweerigmhotniotfoorfefdspirnintgh.e eBvaaslcudateodn inthtehsee srteusdulytsa,rethgeiveNnO(ATEabClesan7d). LTOhAeENCo fOobrseardvualbtleanAddvjeurvseenilEeffeecntdpLoeivnetls (meNaOsAuEreLd)inanvdariLoouwsetsitssuOebsmaetrrviacbelsearAedavlesroserepEofrfteecdt (LTeavbelle 8().LOAEL) values for PFOS 10 GOOG i`nTaacblher7.oMneapsiutresmteudnyt ewnidtphobinotbswhaintdeaqsusaoicliaatneddtdhieeitraorfyfNspOriAngE.C and LOAEC values for PFOS Endpoint * Dietary NOAEC Dietary LOAEC ADULT (pm) MoBrotdayliwteyight Fema"l1es76217.6 FemalNeost=aNppolticaapbplleicable Feed consumption Male6s2~62 Male1s7=6 17.6 Liver weight FeMmaalleess 261.72.6 FemaleMsal=esNo=t a17p.p6licable HiGsrtoospsaptahtohloolgoygy Fema1le7s6> 17.6 FemalNeost=aNppolticaapbplleicable Reproductive Mal2e1s7=66.2 NMoat lapep=slic1a7b.l6e OF1F4-SdPayRIsuNrvGivability 2176 Not applicable JHuavtecnhillienglijvuevrewneiilgehbtody weight E21i76 NNoott applaippclaicbalblee Cwonktroelxpsodsur1e7..Aplplmcoensccsnaaironsserdepoornte3d2n0swecwktewxepoisguhreaiTs.E E7062 pr resus as Based on LeOsAiE.C for males based ona satisticaly significant duction inbod wight, ive weight and histopathology n 600s `inTaablcehr8o.nNicOApEiloLt satnuddyLwOitAhEPLFOvSa.lues in various matrices in adult and offspring bobwhite quail Measures of PFOS Exposure * ADULT MALES NOAEL LOAEL DAoDsIe((ppppmmi)cay) 055729 27060 SLievreurm ((pupgm/m)i) 71239 219909 ADDoUsLeT(pFpmE)MALES 76 ASeDrIum(p(pgm/damy)) 2a0s0 OLFiFveSrP(RugI/Ng)G 137 LYiovlekr((uugg//mgl)) 00519 CAoSlnletroronulcmeanntda1t7i.(u6og/nmm)sgrePpoRrtOedSoInsa wuetsweeigahtsbdaio.n Weck0204,1w3h te 7 ppmresusBr sedon Week 6 Nsor arnedpLoorwteafsfmecetsvsaleudessorasdictvelaoncdsADI ae reportedasditary concentrations. Serumand iver effet vluss OAlkLPFvOaSluceosnfcoerntmraalteisonbatsaekdenonfobmodwyeweeik6g.ht iver weight and histopathology resus. DATA QUALITY Reliability: Klimish ranking = | RGaEllFaEghReEr,NCSEIPS,:Van Hoven, RL. Beavers, LB. (2003). PFOS: A pilot reproductive sRteupdoyrtwiLtahbtRheeqNuoersttheNro.n BUo2b7w2h3i.te. Wildlife International, Ltd. Project No. 454-104: 3M Gsaelrluamghaenrd, qSuPa.il (li2v0e0r1)f.or Eaxntarlaycsitsiounsionfg pHoPtLaCs-sEiluemctPreorsfplruaoyr/ooMcatsasnsSuplefcotnraotmeetrfyr.omCeqnuatirle Analytical Laboratories, Inc. Study No. 023-041 G`amlelmabgrhaenre,, aSlPb.um(e2n0,01a).nd yEoxltkrafcortiaonnaloysfisPoutsaisnsgiHumPLPCe-rEflleucotrroooscptraanycMsualsfosnastpeecftrroommetcryg Centre Analytical Laboratories, Inc. Study No. 023-063. OTHER Last changed: 05/05/04 2 Ghul;