Document pemJNwmMGEK95JDjMLvOkKj1k

ACUTE TOXICITY TO FISH (Rainbow Trout) TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder. Sample was taken from 3M lot number 217. Sample was stored under ambient conditions prior to testing. Purity determined to be 86.9% by LC/MS, 1H-HMR, 19F-NMR and elemental analyses techniques. METHOD Method: OPPTS 850.1075 and OECD 203 Type: Static acute GLP: Yes Date completed: Study completed 2001, report completed 2002 Species: Oncorhynchus mykiss Supplier: Thomas Fish Company, Anderson, CA Analytical monitoring: PFOS measured at 0, 48, 96-hours Exposure period: 96-hours Statistical methods: LC50 values calculated, when possible, by probit analysis, moving average method or binomial probability with non-linear interpolation using the computer software of C.E. Stephan. Test fish age: juveniles Average Total Length and weight: 3.6 (3.4-4.0) cm, 0.34 (0.25-0.47) g Loading: 0.23 g fish/L Pretreatment: None Test conditions: Dilution water: 0.45 m filtered moderately hard well water Dilution water chemistry (during the 4-week period immediately preceding the test): hardness: 130 (128-132) mg/L as CaCO3 alkalinity: 177 (176-178) mg/L as CaCO3 pH: 8.3 (8.2 - 8.4) TOC: Not given Conductivity: 311 (310-315) mhos/cm Stock and test solution preparation: Primary stock prepared in dilution water at 150 mg/L and mixed for ~23 hours prior to use. After mixing, primary stock solution was proportionally diluted with dilution water to prepare the five test concentrations. Concentrations dosing rate: Once Stability of the test chemical solutions: Extremely stable Exposure vessels: 25L polyethylene aquaria containing approximately 15L of test solution; water depth approximately 17.5 cm. Number of replicates: two Number of fish per replicate: ten Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 - 96 hours): 9.4 - 10.7 mg/L (control exposure) 9.2 - 10.8 mg/L (50 mg/L exposure) pH range (0 - 96 hours) 8.1 - 8.4 (control exposure) 8.2 - 8.4 (50 mg/L exposure) Test temperature range (0 - 96 hours) 12.1 - 12.6C (control exposure) 11.8 - 12.9C (50 mg/L exposure) - Method of calculating mean measured concentrations: arithmetic mean RESULTS Nominal concentrations: Bk control, 3.1, 6.3, 13, 25, 50 (exposure), 50 (abiotic) mg/L Measured concentrations: <LOQ, 3.0, 6.3, 13, 25, 50, 52 mg/L Element value: 24-hour LC50 = > 50 mg/L (C.I. not calculable) 48-hour LC50 = > 50 mg/L (C.I. not calculable) 72-hour LC50 = > 50 mg/L (C.I. not calculable) 96-hour LC50 = 22 (18 - 27) mg/L All element values based on mean measured concentrations Statistical Evaluation of Mortality: Element values and confidence limits for 24, 48, and 72-hours could not be calculated due to lack of mortality. Probit Analysis was used to calculate the 96-hour LC50. Analytical Methodology: Analyses of test solutions were performed at Wildlife International Ltd. using high performance liquid chromatography with mass spectrometric detection (HPLC/MS). When determining the concentration of the test substance in the test solutions, the same and most prominent peak response for perfluorooctanesulfonate was used. No attempt was made to quantify on the basis of individual isomeric components. The LOQ (limit of quantitation) was 0.200 mg/L in this study. The mean percent recovery of matrix fortifications analyzed concurrently during sample analysis was 101. Samples collected at test initiation had measured values from 93.2 to 103% of nominal. Measured values for the biotic samples taken at 48-hours ranged from 93.6 to 103% of nominal, while abiotic samples ranged from 105 to 106% of nominal. Measured values for biotic samples taken at 96-hours ranged from 91.4 to 105% of nominal, while the abiotic samples were 102% of nominal. Summary of analytical chemistry data: Nominal Test Concentration, mg/L Measured Duplicate Values at 0, 48, and 96-hours, Respectively, mg/L Mean Measured Concentration, mg/L Percent of Nominal Negative Control All < LOQ <LOQ - 3.1 3.15, 3.02, 2.90, 3.01,2.83, 2.97 3.0 97 6.3 6.22, 6.21, 6.16, 6.43, 6.15, 6.60 6.3 100 13 13.2, 12.1, 12.7, 12.3, 13.1, 12.6 13 100 25 25.0, 25.7, 24.3, 25.7, 25.7, 26.2 25 100 50 49.7, 49.8, 51.1, 51.5, 49.6, 50.8 50 100 50 (abiotic)(1) 53.1, 52.6, 50.9, 51.0 52 104 (1) Samples taken at 48 and 96-hours only Biological observations after 96-hours: Fish in the negative control and the 3.0 and 6.3 mg/L exposure concentration appeared normal with no mortalities or overt signs of toxicity. All surviving fish in the 13 and 25 mg/L exposures appeared normal with no overt signs of toxicity after 96hours. Cumulative percent mortality: Mean Measured Test Conc., mg/L 24-hours 48-hours 72-hours Neg. Control 0 0 0 3.0 0 0 0 6.3 0 0 0 13 0 0 0 25 0 0 0 50 0 5 35 96-hours 0 0 0 20 50 100 Lowest concentration causing 100% mortality: 50 mg/L Mortality of controls: None CONCLUSIONS The potassium perfluorooctanesulfonate 96-hour LC50 for rainbow trout was determined to be 22 mg/L with a 95% confidence interval of 18 -27 mg/L. The 96-hour no mortality and no effects concentration was 6.3 mg/L. Submitter: 3M, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking = 1 REFERENCES This study was conducted at Wildlife International Ltd., Easton, MD at the request of 3M. OTHER Last changed: 1/24/02