Document mqXoQ1yQ01RxRkw9z4jDmGBd
HAZLETON LABORATORIES AMERICA. INC.
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. P.O. BOX 7545 MADISON, W ISCO N SIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL M D S UD
FINAL REPORT
R O G E R G. P E R K I N S MINNESOTA MINING & MANUFACTURING TOXICOLOGY SERUICES ST. P A U L , MN 55101
COMPANY
SAMPLE: T-4102 PURCHASE ORDER NUMBER: T837389-410 754
JAN 2 8 1988
\\ V, >.
BIVI
/
SAMPLE NUMBER: 7090576 SAMPLE ENTERED: 09/25/8 REPORT PRINTED: 01/25/8
ENCLOSED:
A C U T E O R A L T O X I C I T Y S T U D Y IN R A T S (OE CD G U I D E L I N E S )
o Key Personnel o Method o Summary o Individual Pathology Comments o References o Pathology Report o Raw Data Appendix
SIGNED:
S T E U E N M. G L A Z A STUDY DIRECTOR ACUTE TOXICOLOGY
.......... \r^ S ^ DATE
000529
HAZLETON LABORATORIES AMERICA, INC
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. P.O. BOX 7545 MADISON, W ISCO N SIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL M D S UD
SAMPLE NUMBER: 70905761 SAMPLE: T-4102
PAGE
2
KEY PERSONNEL
Acute Toxicology
S t e v e n M. G l a z a Study Director
C a I v i n L. H o r t o n Group Leader Support Services
S h a r e n L. H o w e r y Report Coordinator
Quality Assurance
Dehra Curley Arndt Manage r
Anatomical Pathology
T h o m a s E. Palmer,, P h D Anatomical Pathologist
Robert Salava Senior Section Supervisor
Dennis Hoffman Group Leader Necropsy
Anne Mosher Group Leader Pathology Data
L a b o r a t o r y Animal Ueter inar ian
C i n d y J. C a r y , D U M Diplomate^ ACLAM
000530
HAZLETON LABORATORIES AMERICA, INC.
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. P.0. BOX 7545 MADISON, W ISCO N SIN 53707 PHONE (606) 241-4471 TLX 703956 HAZRAL M D S UD
SAMPLE NUMBER: 70905761 SAMPLE: T-4102
BEST COPY AVAILABLE PAGE
OECD ORAL SCREEN
Objective: To determine the acute oral toxicity produced when a test m a t e r i a l is a d m i n i s t e r e d b y the o r a l r o u t e ( g a v a g e ) to r a t s a c c o r d i n g to the O r g a n i s a tion for Economic C o op er at io n and D e ve lo pm en t' s Guidelines for Testino of Chemicals.
Test Material: T-4102 Physical Description: Purity and Stability:
Storage and Retention:
Safety Precautions:
Dark amber liquid Sponsor assumes responsibility for purity and stability determinations. The test m a t e r i a l wa s s t o r e d at r o o m t e m p e r a t u r e . Any unused material will be discarded according to H L A S t a n d a r d O p e r a t i n g Pr o c e d u r e . Normal handling procedures were used according to H L A S t a n d a r d O p e r a t i n g Pr o c e d u r e .
T e s t A n i m a l : Y o u n g a d u l t a l b i n o r a t s of the S p r a g u e - D a w 1e y s t r a i n w e r e p r o c u r e d , s e p a r a t e d b y s e x , m a i n t a i n e d in g r o u p c a g e s in t e m p e r a t u r e and h u m i d i t y - c o n t r o l led q u a r t e r s , p r o v i d e d c o n t i n u o u s a c c e s s to P u r i n a R o d e n t C h o w a n d w a t e r , a n d h e l d f o r a n a c c l i m a t i o n p e r i o d o f at least. 7 days. Animal h u s b a n d r y and h o u s i n g at HL A c o m p l y with s t a n d a r d s o u t l i n e d in th e " G u i d e for the C a r e a n d U s e of L a b o r a t o r y A n i m a l s " . If v a r i a t i o n s f r o m t h e p r e s c r i b e d e n v i r o n m e n t a l , c o n d i t i o n s e x i s t e d , t h e y w e r e d o c u m e n t e d and c o n s i d e r e d to ha v e no ef fe ct on the studs' o u t c o m e . No c o n t a m i n a n t s w e r e e x p e c t e d to h a v e b e e n p r e s e n t in the feed or water which would have interfered with or affected the results
of the study.
A c c l i m a t e d a n i m a l s we re c h o s e n at r a n d o m for the study. Test a n i m a l s w e r e h o u s e d b y s e x in g r o u p s of f i v e a n d i d e n t i f i e d b y animal, n u m b e r a n d c o r r e s p o n d i n g e a r tag. F o o d a n d w a t e r w e r e a v a i l a b l e ad. l i b i t u m throughout the study, except for an overnight period just before test material administration when food, but not water, was withheld.
R e a s o n for S p e c i e s S e l e c t i o n : T h e rat is the a n i m a l c l a s s i c a l l y u s e d d u e to its small size, ready availability, and large amount of ba ck g r o u n d data.
Me tho d: F i ve ma le and five f e m a l e rats w e i g h i n g fr om 200 to 2 8 4 g w e re used for each dose level. The study consisted of two dose levels (0.50 and 5.0 g/kg).
P r ep ar at io n and Administration of Test M a t e r i a l : An individual dose was c a l c u l at ed for each animal based upon its fasted body weight and administered undiluted by gavage.
The dose volume of the test material varied per dose leuel-based upon an average bulk density of 1.06 q / m l .
000531
HAZLETON LABORATORIES AMERICA. IWC.
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. P.O. BOX 7545 MADISON, W ISC O N SIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL M D S UD
SAMPLE NUMBER: 70905761 SAMPLE: T-4102
BEST COPY AVAILABLE
page
4
OECD ORAL SCREEN
(CONTINUED)
R e a s o n for R o u t e of A d m i n i s t r a t i o n : T h i s is t h e m e t h o d for a d m i n i s t e r i n g a known q u a n t i t y of test su bs tance and has been the route of choice historical Iy.
Observations: The animals were observed for clinical signs and mortality at 1, 2 . 5 a n d 4 h o u r s a f t e r test m a t e r i a l a d m i n i s t r a t i o n . T h e a n i m a l s were observed daily thereafter for 14 days for clinical signs and twice a day for mortality.
Al l a n i m a l s w e r e w e i g h e d just b e f o r e test m a t e r i a l a d m i n i s t r a t ion, at 7 d a ys and at s t u d y t e r m i n a t i o n (or at de at h).
Pa t h o l o g y : At s t u d y t e r m i n a t i o n s u r v i v i n g a n i m a l s we re e u t h a n a t i z e d . A n i m a 1s w h i c h d i e d o n s t u d y o r e u t h a n a t i z e d at s t u d y t e r m i n a t i o n w e r e s u b j e c t e d to a gr os s n e c r o p s y e x a m i n a t i o n and a b n o r m a l i t i e s re co rd ed . Following necropsy, animals were discarded and no tissues were saved.
Statistical Methods: Other than average body weights, no other statistical me thod was pe rf ormed.
L o c s t i o n o f R a w D a t a a n d F i n a l R e p o r t : T h e r a w d a t a a n d a c o p y of th e final, r e p o r t w i l l b e r e t a i n e d in th e a r c h i v e s o f N L A ,
000532
HAZLETON LABORATORIES AMERICA. INC.
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. P.O. BOX 7545 MADISON, W ISCO N SIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL M D S UD
SAMPLE NUMBER: 70905761 SAMPLE: T-4102
BEST COPY AVAILABLE PARE
OECD ORAL SCREEN
(CONTINUED)
SUMMARY
T e s t A n i m a l : A l b i n o R a t s - S p r a g u e - D a u i 1e y s t r a i n Source: Charles River Laboratories, Inc.; Portage D a t e A n i ma 1s R e c e i v e d : 0 9 / 0 8 ; 1 0 / 1 2 , a n d 1 0 / 2 6 / 8 7
MI
Method of Administration: Oral Gavage
Date Test Started: 10/26/87
Date Test Completed: 11/20/87
E s t i m a t e d O r a l L.D5 0: M a l e - B e t w e e n 0 . 5 0 a n d 5 . 0 g / L g o f b o d y w e i g h t Female - Between 0.50 and 5.0 g/kg of body weight
Dose Leve 1 (g / k g )
Ma 1e
0.50 5,00
F e m a 1e
0.50 5.00
A v e r a g e B o d y Lie ig h t s (g )
Initial
Day 7 Terminal
272 255
273
--
339
--
210 216
198
--
238
--
Mo r ta I ity (Nu mb e r Dead/Number Dosed)
2/5 5/5
0/5 5/5
000533
HAZLETON LABORATORIES AMERICA, INC.
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. P.O. BOX 7545 MADISON, W ISCO N SIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL M D S UD
SAMPLE NUMBER: 70905761 SAMPLE: T-4102
am p
OECD ORAL SCREEN
(CONTINUED)
CLINICAL SIGNS (No. of A n i m a l s Af f e c t e d )
_____ H o u r s _____ 1.0 2.5 4.0
______________________ D a 'os_______ ___________________________ 1 2 3 4 5 6 7 8 9 10 11 12 1.3 1 4
DOSE LEUEL: 0.50 g/kg of body weight
Ma les
Appeared normal 5 5 5 5 5 5 4 0 0 3 3 3 3 3 3 3 3
A g g r e s s iv e
behavior
0 0 0 0 0 0 3A 1 1 0 0 0 0 0 0 0 0
H y p e r s e n s it iv i t y
to touch
0 0 0 0 00 0 12 0 00 0 0 0 0 0
H y p o a c t iv i,t y
0 0 0 0 0 0 02 1 00 0 0 0 0 0 0
Intermittent clonic
convulsions Dea th
0 0 0 0 0 0 0 0 IA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 B 0 O' 0 0 0 0 0 0 0 0
Ferna 1es
Appeared normal 5 5 5 5 5 5 0 0 0 3 3 3 4 4 4 4 4
A g g r e s s ive
behavior
0 0 0 00054 0000 0 0 0 0 0
H y p o a c t i.v i t y
0 0 0 000011000 0 0 0 0 0
H y p e r s e n s i t iv ity
to touch
0 0 0 000024 111 0 0 0 0 0
Ataxia
0 0 0 000011000 0 0 0 0
I n t e r m i t t e n t c i o n ic
c o n v u I s io n s
0 0 0 00002 000 0 0 0 0 0 0
A 1o p e c ia -
back region
0 0 0 000011111 1 1 1 1 1
A - Sign noted after a.m. observation. G - One animal found dead after a.m. observation.
000534
HAZLETPIN LABORATORIES AMERICA, INC
3301 KIN SM AN BLVD. P.O. BOX 7545 MADISON, W ISC O N SIN 53707
Chemical & BioMedical Sciences Division
PHONE (608) 241-4471 TLX 703956 HAZRAL M D S UD
SAMPLE NUMBER: 70905761 SAMPLE: T-4102
iEST COPY AVAILABLE PflEE
OECD ORAL SCREEN
(CONTINUED)
CLINICAL SIGNS (Continued) (No. of A n i m a l s A f f e c t e d )
_____ H o u r s_____ 1.0 2.5 4.0
______________________ D a y s ___________________________________ 1 2 3 4 5 6 7 8 9 10 11 12 13 14
DOSE L E U E L : 5.0 g/kg of body weight
Ma 1es
H y p o a c t iw i ty
5 5 5 52 00
Miosis
4 0 0 0000
A ta x ia
2 1 0 0010
R e d - s ta in ed
face
0 0 0 3100
L a c r ima t ion
0 0 0 2 000
Yellow-stained
gen ital region 0 0 0 0 1 0 0
Pa in r e f l e x
absent
0 0 0 0100
Diarrhea
0 0 0 0010
Ton ic
conwuIs ions
0 0 0 0010
E x c e s s iwe
s a 1 iv a t ion
0 0 0 0010
Death
0 0 0 1A 2 1 1
F e m e 1es
Appeared normal 0 0 0 2 0 0 0 0 0 - - - - - - - -
H y p o a c t iw i ty
5 5 5 1 01000-- - - - - - -
Miosis
5 0 0 000000
Ataxia
1 2 1 10 1000-- - - - - - -
Ye 1 low-stained
--- - - - -
genital region 0 0 0 2 1 2 2 2 0
G a s p ing
0 0 0 100000--- - - - - -
R e d - s ta ined
--- - - - - -
face Resp iratoru
0 0 0 111000-- - - - - - --_ - - - - -
congest ion
0 0 0 100000--- - - - - -
Diarrhea
0 0 0 01 00 00-- - - - - - -
Tonic
--- - - - - -
c n n w u I s ion s
0 0 0 0 0 102 0-- - - - - - -
Deat. h
0 0 0 1A 1 0 1 1A 1 - - - - - - - -
A - F o u n d d e ad at p.m. m o r t a l i t y check.
OOO;
HAZLSTOhl LABORATORIES AMERICA, INC.
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. P.O. BOX 7545 MADISON, W ISC O N SIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL M D S UD
SAMPLE NUMBER: 70905761 SAMPLE: T-4102
BEST COPY AVAILABLE
DEED ORAL SCREEN
PATHOLOGY
DOSE LEUEL
0..50 g / k g o f b
An ima 1 Number
Test Day Sex Died Sacr if
COT 081
M
4
-
COT 08 0
M
4
COT082 COT 079 COT 088 C0T249 COT247 C.nT25 0 C0T248 C0TT2
M M M F F F F F
14 14 14 14 14 14 14 14
(CONTINUED)
w e igh t
Necropsy Comments No visible lesions. Uentral cervical region and both mandibles cannibalized. No visible lesi o n s . No visible lesions. No visible lesions. No visible lesions. No visible lesions. No visible lesions. No visible lesions. No visible lesions.
PAGE
8
00S36
HAZLETON LABORATORIES AMERICA. INC.
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. * P.O. BOX 7545 MADISON, W ISCO N SIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL M D S UD
SAMPLE NUMBER: 70905761 SAMPLE: T-4102
BEST COPY AVAILABLE PAGE 9
OECD ORAL SCREEN
(CONTINUED)
PATHOLOGY (continued)
D O S E L E U E L .: 5.,0 g / k g o f b o d y w e ig h t
A n i.m a 1 Numbe r
Se V
Test Day Died Sacrificed
Necropsy Comments
C10499
M
4
Perineum stains - tan; stomach glandular portion has dark brown f o c a l a r e a s (1 m m in d i a m e t e r ) .
000504
M
3
Perineum/perianal stains - yellow: nasal discharge - dark red and crusted.
C O 0503
M
2
Nasal and mouth areas - stained brown,: s t o m a c h - g l a n d u l a r p o r t i o n has dark brown areas (up to 5 x 1 m m ).
CO 05 01
M
C00562
M
1 ic-t.
Stomach - glandular portion has multiple,, dark brown, pinpoint foe i.
Stomach - glandular portion has dark br ow n areas (up to 2 x 1 mm).
C 03 035
F
2
No visible lesions.
C03034
E
4
Stomach - glandular portion has dark brown areas (up to 3 x 1 mm).
C 03 03 2
F
6
--
Stomach - glandular mucosa has d a r k b r o w n a r e a s (up to 4 x 1 rnm) .
C03029
E
1
No visible lesions.
003038
F
5
Perineum stains - brown; paranasal d i s c ha rg e - red.
OOOS37
HAZLETOtM LABORATORIES AMERICA. INC.
Chemical & BioMedical Sciences Division
3301 KIN SM AN BLVD. P.O. BOX 7545 MADISON, W ISCO N SIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL M D S UD
SAMPLE NUMBER: 70905761 SAMPLE: T-4102
BEST COPY AVAILABLE PAGE 10
GECO ORAL SCREEN
(CONTINUED)
Re ferences:
1. O r g a n i s a t i o n f o r E c o n o m i c C o o p e r a t i o n a n d D e v e l o p m e n t ' s G u i d e l i n e s for Te s t i n g of Ch emicals, Se ct i o n 401, Acute Oral Toxicity, a d o p t e d May 12, 1981.
2. O E C D ' s P r i n c i p l e s of G o o d L a b o r a t o r y P r a c t i c e , A n n e x 2, C ( 8 1 ) 3 0 (Final).
3. N I H P u b l i c a t i o n No. 8 6 - 2 3 ( r e v i s e d 1 9 8 5 ) .
000538
HLA LAB NO. 70905761 PATHOLOGY REPORT Acute Oral Toxicity
PAGE 11
Ten rats (five males, five females) from each of two dose levels (0.5 and 5.0 g/kg) were necropsied. The animals that died on test were refrigerated and necropsied within 24 hours, usually the same day. All surviving animals were euthanatized and necropsied at tne termination of the study. The dose level, day of death, and gross observations recorded for each animal are on pages 8 and 9 of this report.
The most frequently recorded observations were in those animals that died on test. The mucosa of the stomacn (glandular portion) of several animals had dark brown areas of variable size which were possibly treatment related. All other observations were considered related to postmortem change or incidental findings which were not related to treatment.
Pathologist (1734mcs)
Date
000539
Personnel Signature Sheet Acute Toxicology
HLA NO. l O S O S I t p l
Name
J-Qb Title
Signature
Becky Beckwith Steve Beloungy Ken Bridges Pat Crary
Shane E1th Shawn Frazier Steven H. Glaza Nolly Hahn Kevin Hamilton Jeff Hicks Calvin Horton Sharen L. Howery Gregory Johnson Paul Krebs Mayne Nad1son Scott HcConnell Shelley HcConnell Dawn Conant
Don Navis Robin Olson Patricia Padgham Joseph J. Daun
Nlchael Patzka John Paulson Jane Polnow Dennis B. Steiner Nlchael Theslng Paula 6.. Vangen Albert Olson Jim Jirschele Ben Haley
Sr. Lab. Animal Asst. Lab. Animal Asst. Sr. Lab. Animal Asst. Sr. Lab. Animal Asst. Lab Animal Asst. Lab Animal Asst. Group Leader Admin. Clerk Lab. Animal Asst. Sr. Lab. Animal Asst. 6roup Leader Administrative Asst. Lab. Animal Tech. Lab. Animal Asst. Section Supervisor Lab. Animal Tech. Lab. Animal Tech. Sr. Lab. Animal Asst. Lab. Animal Asst. Lab. Animal Asst. Team Leader Lab. Animal Asst. Lab. Animal Asst. Sr. Lab.' Animal Asst. Lab. Animal Tech. Lab. Animal Tech. Lab. AnlmalAsst. Administrative Asst. Manpower LTE
Sr. Lab Animal Asst.
G l zi
j Bs L U ICu i-
'Tt&untljnOL IKiALL| M Q W jM
IMUtA > vd& kci*7 __ iA i O T M
ifb tt V J
Initiais a G > S. j _ ft-
..
-C A L ___
Skc.
Jz2rZ %r
r/vr
HLA: ' l o ^ O ^ l C a l
DOSE ADMINISTRATION/BODY UEIGIIT/HORTAUTY RECORD
Study Tltlei Acuta Oral Toxicity
Test Material: l ~ I O ^ -
Vchlcte; n a
1,/,z/p
Bulk Density; \ . o C m (g/mL) Speclei/Straln: Albino Rat/Sprague-Dawlcy Source:__Charles River Dele Received; Iojz(oj^1 %
Dose level; O . S^**)(o/kal Fasted Date/TIma/Technlctan 11
p../
Root llo. -S~~
Route of Administration; Oral Gayage
Dose Volume 0 . M f (ml/kg)
Doso Timo: \{:
Technician
Scale Usedj \ T ( W
o
o o C/i
p
BEST COPY AVAILABLE
Dose 1evel ig/kgi. Sex o /T L
lech. Date \ % 1 NA - Not Applicable * - Dosage calculated, but not administered
unused animal returned to stock
Reviewed By C ^ a O Data J,Q Un P, ~7
--- R T F i T /ST Pm
NA
NA */ <r
NA 0 4 k NA " f z o
BEST COPY AVAILABLE
OBSERVATIONS (Individual) Dose level: < D ______________ Test material: 1 ~ *~l 1 ^ 2 --
HLA:"1C^?(3$~7fei
i____
>1 y j
Surviving animals submitted for terminal
Ou-necropsy.
Technician
Date
11
Surviving animals designated for sacrifice
and discard. .a Technician N n _______
Date N//V____________
Indicates condition exists
Si Slight.
- Condition not evident. * Found dead, P.M. check.
a m 'Nor Reviewed by: -laf)_______ Date: 3 ((t'W/
BEST COPY AVAILABLE
Dose level:
<Q - ^
//
OBSERVATIONS (Individual) Test material: I " IO Z .
H LA :lo3o 74[
evvO^is
Surviving animals
submitted for terminal
necropsy.
n,
Technician /
Date
11
Surviving animals designated for sacrifice
and discard. A Technician N n ______
Date K/&
Indicates condition
exists
SI Slight.
- Condition not evident.
* Found dead, P.M. c h e e k y
u A ' / m /?/*?/
r} ^
Reviewed by:
Date:
DOSE ADNINISTRATION/BODY WEIGIIT/HORTAUTY RECORD
HLA: 1Q<toS n U . l
Study Title: Acuta Or) Tonicity last Material: T - A l O a __________________
Vehicle: X4
Bulk Density: / h(,, (g/wL) Spectes/Straln: Albino Rat/Snrague-Dauley Source: Charles River Data Recalved: io/i'2-^7
Dose level: S.O _____(g/kg) Fatted Oata/Tlaw/TechnlcIan AQ^iW/&57 2:,3>o n .w./ A ? S
Route of Adwlnlstratlon: Oral Gavage
r ffa k h l
Room Ho . S
BEST COPY AVAILABLE
Dose Yolmae - ^ Iwt/kg)
Animi Ho.
Co
Sexir'TluVemla
o<&! 0 ^ 3 0 ^ 0 1
Technician
(0 6 -
Sff- Scale U s e d K ^ ^ I
NA
Protested Body Height (el M A _________ ________ ________________
Fasted Uody Height (g)_____2 * * ~lL 2 4 * ^ - "LioO 7J}^\ 2 - ^ /
Actual Dose Day 7 Uody Weight (g)
1,3,.
C M PpL
OcaJ
J
A
*
_AA_
Qd-Jl
o Day |4 Body Weight (g) o Dead Body Weight to) o
C1
m r y T \ v 4 o itsiAi-rt \ z i X
Sea: llale^fcrnle^
Anlml No.
Ca D
Prefasted Body Weight la)
Fasted Body Weight (g)
3o3f 3o i Z
Nk/HA .__
2 - 1 ? Z.2.0 -22-1
yx
2_|4
*a!>8 \ \
21 1
\
\
Actual Dose (m L) Day 7 Uody Weight (g) Day M Uody Weight (g) Dead Body Weight (g)
1.0
P 7*A Tmm
1.0
A S J35L
l.o na
1.0
wr
#
ZoT-
a 87
itol
\
Dose
ours
level
la/LaL Sex
s.o cf /s
6.0 ? %
HORTALTY (NO. DIED/HO. DOSED) Study Bay
lech.
m
iso
VeFIFTeW "by: 77/
Technician
/%L
Q&7
Date
tofu*
Scala MsnfcffbjJ NA
S o I*?
va rilla d by:
77/ /o 'jc /'7j
Q)C.il<y rrcv j iojju /ri
&&nhfcrrcr CorrtctrJ /of'' '%?/#/
(3) enVvM. evrdrS o )\
(xyv-rA^Jt^
o\\
\
oh
1
0
,70 H - t -27
Date
NA - Not Applicable * - Dosage calculated, but not adailnlstered
unused anlaul returned to stock
Reviewed X-J^bsi-- P*te iQ A U & l
OBSERVATIONS (Individual)
Dose level: 5 . 0
Test material:
___________
rftaT^Female
Animal
pre
Hours
Number
Co-
CHM
Observatl ons Appeared Normal
hypwHifC a >drTnrk C ^r CoiAV*Uito>\5 (
MfDts^X 1^
s aXCtK5>V< l&itVslJ-ion
doSS. 1 2 . 5 4 1 2 3 Z 77
7
x
Appeared Norma!
tX
& > - b pa atVa/e
PUc.frVi.-- .-- ___
C ' t t in e d J l& Z .
05o^j
yfe/ruvf yifc rgQjgy
Oo
o Appeared Normal
ITi *
Co-
en
hy atliii_
ThVqp'^. Sr>gg| -/ c g
603
________
1 *1 X
X
77
1/ - vT
X
7
7
Study Day
8 9 10 11 12 13 14
CoC&o Po- '
Appeared Normal
uTivfm ftVios_____ ______ i/ifriy*sLT,oyy
pa h * 7 7
Appeared Normal
0S<*L
V\yp^igeila
miar*
or i j d
Deaths Collected by late
/?.s;
1Z1 71 7. 7 x 7
7
t7it
X
7 7
jX x
7 T 77
j
HLA: l o g o 5 1 U
n sa rs" <yj
- Surviving animals submitted for terminal necropsy. Technician
Date
M A?
- Surviving animals
designated for sacrifice
and discard. Technician
N. .fav___
Date
N f t __________
Indicates condition
exists SI Slight.
- Condition not evident.
* Found dead, P.M. check.
m mr
n*
Reviewed by: Cjhn _____
Date:
7i7
OBSERVATIONS (Individual)
Dose level: 5 . 0 ^ / K g
_Test Material: 'T-^iOJ._____________
Animal Number flo-
33S
Hale/j
Pre-
dose
Appeared Normal
!Z
y^aaihih-.
Aligns
(yrilhds
Z
z
Hours
2.5
Study Dan 8 10 11 12 13 14
Appeared Normal
77
Co tyfiOAirtii.Ve
aiLuii
O to r d h m i*a
O
twr.
3-3</ a,~T>-Xi<-
uLU.
Appeared Normal
ff do-
~ TiAUi.
^h
303c3
* W \ c
te-iin<J yAi k/r-ayC
ConviviJjSlor\!>___
1
z
y
z _Z_ z
z
\/ 77 Z 77
7
Z Z
Appeared Normal C<r- hys*acfi\
, Z fc
Z.
3M)
JMftri'atAs 3
i Appeared Normal : -
JlTa/i
33y r t sfptirrsSttoorryy C go>An<yi?e)hK 3 < y * w 0/\
z zz
zz z
7~
z
z z 77
Z z 77 Z
Voavc. CoviV if f ls ig n <. Deaths
77 7 7
r r J
Collected by
Date J3Z- w m m w m m w i t m w m i B i
(V.Z O 'tor m * W f 7
H L A :_ T 0 fi0 5 Ik ! , --'S
Surviving animals
submitted for terminal
necropsy.
^
Technician v J n
Date
HA-
Surviving animals designated for sacrifice and discard. . a
Technician r J n _______
Uf\Date
Indicates condition exists
SI Slight.
- Condition not evident.
* Found dead, P.M. check. m - A i d T / j / y y / c m / i - /-/> e f y o
Reviewed by: r'^ p _______ Date: Q-\u 6 t
