Document kmqzbDdoERKwwGRgYzMRrX5LE

Younger Laboratories Biooktmliti.... PltamaadayiiU.... Attahfiii 198 CLIFF CAVS ROAD Saint Louis, Mo* esxae PBORII TlLOBN 6*3560 Ce/dificcde &j AnalfUb Auguat llth, 19*4 SUBJECT Toaloologlaal laraatlgatloa Ofi Fydraal 2*0 Koaaaato Sanpla Noabar 102 Koaaaato Projoot Nunbar 1-64-91 STBDI C0N00CTB) 102 Koaaaato Coapaaf, 3t. Louie, Mlaoouri JXmXMatTAL FgOCBUU A) Oral UD,0 (2ato, Mlnod S< Tha uadtlutad ooapouad albino halo aatUMSlo Aubo to Sprogua-Qaalay atrain Aftar Um aad faaalO log inta. uppljrlag odlflaatloa ato HIUMm Lothadifaao oaa dotamiaad, groupo of aala fad lrtanwng doaaa at laaraaaata of 0.1 fraotloaal four loTold^doarfaod to blaakat tha toaialty raago tharaby (ttloa of tho U>,0 which waa doao aaoordlag to a ` of I. J. do Boar. Ohaorwatloaa ooraaadb for tonlo ayaptoaa and tho wlaaora of tho "* --i that aaoewahad woro awalaod aaoroaoapl pally. Tho data ara ahead in Tahlo 1. B) Skin Abaorptlaa MU) (Babbita, HUad Baa) Tho undiluted ooapouad waa appliad la laaroaalag doaaa at laeraiaata of 0.2 fraotloaal log Intervale to tho oloaaly elipped, lntaet akin of Moo daalaad ohlto aala aad faaalo rabblta. Tho traatod araaa woro aoworod with plaatla atrlpo aad tha aniaala plaaad la woodee atooka for parloda up to toaatjrfour hoara, aftar whleh tlao they war# aaalgaad to Individual oagaa. Obaarwatloaa aaro aada for tonlo ayaptaoM aad tha vlaoara of tha aalaala that aaaoaahad woro onaalaad aaaroaaoplaally. Tha data ara ahoaa la tabla II. MONS 037878 Thia aoofidoatiel Mport la oot to bo uaod lo airy form of advertiatng without writtao poimiaaioo ToI Monsanto Compan. St. Louis, Missouri lounger Laboratorlsa Certificate of Analysis - Pag* 2 (8/11/64) - r-44-91 KX/KAIMcNTAL PHOCAUBRli - (Continual) C) Skin Irritation (Babbits) Th undiluted oonpound was applied to tha clipped, intact akin of alblao rabbits and rsnorsd aftsr twenty-four hours. Tha application was covered with plaatia strips to retard evaporation and avoid contamination. Observations wars sada ovar a period of several days for irritation. The data, soared accerdinf to the oothol of Uraise, Woodard and Calvary (Journal of fhar*. and ffltp. Therapeutlos, Voluae 82, Oooaabor, 1944) are shorn In Table III. 0) l^re Irritation (Rabbits) 0.1 Milliliter of undiluted saaple ens plaaed la the conjunctival sas of the right eye of sash of three albino rabbits and observations sale over a period of asvernl days for lnflcarnation. The syce vers rinsed with vara isotonic saline solution after twenty-four hours. Tha data, scored according to the aethod of Jraise, et el, are shown in Table IV. 1) Vapor Inhalation - 350* /. (Mala Rats) din nature sals rats aero placed la a aetal chaaber of 35 liters capacity end exposad for sis hours to a concentrated ataoephere of vapora produced by passing a streaa of air through 130-alllllitars of tha eoapount csatalasd in a JOO-ailllliter Arleaneysr flank pinned la n aaad bath oa a hot plate and the contents aalatalaad at a teaperature of 330* f. Vapors tram the flask passad lata a train of tea ana-liter bottles to reaove droplets and than late the ehaaber. Air floe through tho aanple ase flva liters per alaute as aaasurad by a calibrated rotaaeter. This ana sufficient to violently agitate the liquid. Me cuppleacatary air ana introduced laaaaueh aa the above supply sas aapla for tho aalaala oxygea requirements. The salsals were observed for behavior sad, slass there were ao deaths, all wars held for tan days observation. Thw data art shorn la Table V. SOMNAR - SteslJK A) Oral LOyo (Rats, Mixed Sea) The Oral LDJ0 for sale and faaals rata was placed at 11,730 allllgrans par kllsgraa with lowar and upper Units of 9,370 to 14,390 aUllgmas par kllsgna. The conpound ana classed as pmetloally non-toxic by oral lagsatlea la sale and foanlc mts. HONS 037879 Tot Moaaaato Coapanj it. Louie, Ntaaourl lounger Leboratsrlee Cartlftaata of Analysis - Faye ) (i/ll/t*) - T-&4-91 SUMMIT (Ceatlnued) 1) Skim Ahaerptlaa MLD (Babbits, Mixed Sax) The Hlnlaua Lathal 3eae by Skim Absorption la aalt end feaala rakblta ai feaad to ka (raatar tbaa lf<0 uilllyramo par klleyraa aad lu> tkaa 2000 Tini|naa par klleyraa. The oeapaund aaa olamsad aa aildly taxla by akla ahaerptlaa la aala aad faaala rabbits. C) Skla Irrltatloa (Jlakhlta) Bw ooapeuad was elaseed aa a aodsrute akla lrrltamt. Tka averaye aorta-- aaora aaa 5*> eat af a paaalble la treatyfear hours. D) $re Irrltatloa (lakklta) Xka esapesaA aaa classed aa a aodarete aya irritant. Tha averaye --rtaua aaora aaa Jl. J aat af a poaalkla UO la taeatyfew kaara. I) Taper lakalattaa - J50* F. (Male Bata) All --1* sarvlved tka ala koar aapaaaro aa wall aa tka fallaatay taa fay observation period. It aaa soaeluied that tha rapara aara rilfijr taxi# under aoaAltlaaa af tka teat. Toroua ubobatoubi Tha material in this report is to be used in development of the product and may be given to reeponsible sales contacts, but it is net to be used by them in cdvatf.itriog copy. The source of thia materia! in not to bo divulged until it appear* m formal publications. No exeeptmue to t'm R.'.tvMlnhod rule may be mado without u? aj-proval of tha Medial Depcwt. 'gavdmff matter* of toricity are to be refet.*cd os before to die Medital Depart meat iq St, Louie for reply. * Me&aaate HONS 037880 Toi itaiutt Caafea* St. leuia, Maaearl Teuager Laheratarlea Certificate of Aaal/als - page 4 (%/U/(4) - T-44-J1 tmi i m OKAJL LD,, or 'Pjdraal 280' 102 Ml Saaplo fad ladllatod 4*iwl Me* - fee weight 9a. Daaa ...... Ihte If r--la a- r--1# 5* Mala 4* Mala 5- foaali (- roaal* 7* Mai* t- Mala 5- r--la 10* r--la U- Mala 18* NUa 15- roaala Id* f--la IV Mala Id* Mala IT* r--la Id- r--la Id Mala 2d* Male 220 7,540 250 7,540 245 7,540 *55 7,540 225 7.540 250 10,000 250 10,000 *45 10,000 215 10,000 255 10,000 250 12,(00 2(0 12,(00 22$ 12,(00 210 12,(00 240 12,(00 *55 15,800 210 15,800 22) 15,000 240 15,000 *50 15,800 aarrlral Sarrlrtd aarrlred Mad darrlred aarrlred aarrlred aarrlred Sarrlrad filed aarrlred Mad Mad Mad larrlrad Died Mad Mad darrirad Mad oidcnwaioa - Ike Oral U>M far aalo art faala rata aaa flace* at 11,790 atiltgr-- far MImim with lavor aad affor llodto af >,578 da 14,550 nitgr-- far Irtlopao Om ooopoaad aaa alaaaad aa feaetteVt/ aaa tcrlc tf oral lagaatiaa la la aad foaala rata. aarrlral tlao aaa aararal hoar* to ala dafa with aoat daatta eecarrlag la thro* to fear daja. fade a/aft-- laaladad 1-- of affotlto, aororo diarrhea, laercaalag ooakaoaa, aad aoUayao. It aatofar thar* -- roaal aad llror hffaroala aa wall aa tafia--ttaa af the gaatrle aaaaaa. 037881 Sat Meuaalo Caarai. lt< Lamia, Nlsaaarl laaagar UktnUrlM CartlfUata t Aaalpala - raga $ (J/U/<4) - l-44-n T A l I II TBS Hamm MC1A1. DOSS or 'PpArattl 280' II SKIM AlSOimoS II MUXS laafla Appi.UA OaAilataA Aalaal *a. - a 1 - Mala 2 - raaala ) - Mala 4 - Uaala J - Mala 4 Faaala faLgtt la. 2.7 2.3 3.0 2.4 2.4 2.1 m?TL. 1,240 2,000 3,140 3.010 7,940 12,400 (alftt daaga 3 Oapa tatar - 0.1 - 0.2 - 0.4 - 0.3 - 0.4 - 0.3 nta araiaaA DlaA -- Oapa BlaA -- T Oapa 01aA -- 1 oapa 01aA -- 3 Oapa QUA -- 7 Oapa Iffff DX8CW5I0I - Tla Alai aaa Lathal Oaaa Ip Ui rtiwptlw la aala aaA till paMlta aaa faamA ta la fraatar tkaa lM aMllgtaaa far ttlaffaa aaA laaa ttaa 2000 allllgiaaa far kUaa*a> Ha aa^aaaA aaa aXaaaai aa sllAlp taala If Ala alaarfUaa la aala aaA faaala nkllta. tarrini ttaa aaa lira ta attfrt Aapa. Saala apaptaaa laalaAaA niaail appatlta, aaakaaaa la taa ta tkraa Aapa, aaA aaaaalar lapimat parttaalarlp aatlaaalla ta tha aaah aaaalaa aaattac tta haaA ta Araap. At aatapap tiara aaa llaar Alaaalaaattaa uA palaaaapp lyparaaU aaaraaaaplaallj HONS 037U82 Tat Haaaanto Coapai Leula, Mlaaeari Tmacar labaratorlaa Carhll&aaha af Aaalyaia - Pi|t < (a/lX/44) - T-&4-91 tmi in skin iumnoa in wuuin Aim applicahoh or 'Pyiraai 2*0* taqla AppllaA Uadllata* 9S&U Naaarlaal Sralaatlea At & M 01 LSffltf iLSssa JLSshs 12-Ssms w l*Jsm 1 22 00 2 J2 10 5 52 10 ATarawa X.) J.J 2.< 2.0 0.6 0.0 DX2CBSIX0N - Iha aaapaaaA aaa alaaaa* aa a aadaraha ridi lrritaah. ka nania aaara m 5.) nt af a vaaatkla la tiaatgrtw haara. Attn aaa haar than aaa tanl/ ymtptltla ha *all~4aflaa4 raiaaaa Hhh aa adaaa. aH-4aflaa4 ha aaharaha uryllnaa alhk vary aHjkh adaaa la tiaitf faar haara intoaA aa aaaraca aaara af J.). fallaalaa raaaral at tha aiagaaa* tha aaaaaaa aaara rMhiaat ha O.i la flra Aaya ah ahlah hlaa haral} paraaptibla iijthi aaa ahlXX praaaah aa haa aalaala. HONS 03788J ToI Mooaaato Cobpas St. Laula, Hlaiourl Touagor labor*tori#* Cart!float* of Aaalgala - Pag* 7 (8/ll/<4) - T-<4*91 TAIL* IV ATI IRgmnON IH BAMITS ARQ APPLICATICM OP 'Pydnal 2SO' Saapl* (0.1 NimUUr) Appllag DndllutoA *8tBi.gaftg Naaarlaal Afalaatloa At Th* JM Of 1 Soaf ILSsxs JLSSSS 72 Hour* 120 Hour* 1<S loan i 55 29 21 15 9 4 2 JJ 25 is 11 4 2 5 2( 19 12 S 4 0 Avaraga 51.5 24.9 17.0 11.9 5. 2.0 SUOMSICS Ik* aoapaaaS *u olaaa*4 aa a aadarat* apa irrltaat. Dm avatags aavlaaa aaara aaa $1*9 aat af a paaalbla UO la t*aatg*faar haaaa. CaaalAarakla dlaaaafart AavalapaA fallaalag adalalatratloa. Sllgkt adaaa aad dlaafcarga, aatanta wrptkaaa ef tha palpabval eoajuaatlaaa, aaS alii lila aaagaatlaa aai aotmaal dwllaaaa vara aatat atttda aa hear. Haaa aad Alaakarga Alaappaarad araralgkt. Iapravaaaat aeatlaaal fallaalag lnlgatlaa 1aadlag ta aa araraga aaara af 2.0 la aaraa gaga. oil*** Toi Noaaaata Caapaa, It. Loula, Nlaasiul Tm|w Ubtittaciw CtrUflwI. of Aaalgaia - hfi (l/U/H) - r-i^n TAILS ? INHALATION OF 'Py*a 2K' VAF0N8 IT HAH (350* F.) Ann|< Taparatura Inal*# Chaahar ...................................... ATraf* IiUUt* haUlt; IaaiA* Chuhrn-............................ 79* F. )J * Awut Of laapla -- T* start................................................. 150.0 h .................................................................. 131.0 a Vaparlaa* ar laft la apparataa......... 7.9 Oafcaaat* la lat lltartattla........ 10.5 Caalaaaat* la M litarkattla......... 0.0 aa aa(51) *-* --ais------------------ 1 - Mala 2 - Mala 9 - Mala 4 - Mala 9 - Mala 4 - Mala torltal ivrtfM sarflra* lanlnl Sarrlva* Imltal Ma*art# Aiaaoafart la thirty aAaataa .. Oaaaalaaal Inragalar traathJj .. MaAarata aaaal Alatharga ... taM aaakaaaa tat at laagar at aallaaaa. D1MSUX0N - All aunrlta* Ut ala haar aiptaar* aa aall aa tha falltalag tta tap ahaaraatlaa >trial. It aaa aaaalata* that tha vapara aaa* aUAlg taala aa*ar oaAltlaaa af tha taat. A taaparatara af 550* F. aaa raiiha* la inradaMj tmlgr alaataa. Tha hlaa talar ahaaga* ta salt (rata aa* mtallf taaaa* ta light >ia la faar kwi. A ulamta Aasraa af tua aaa yraaaat la tha itastir Ihmghaat tha taat. Maaaafart laaraaaa* alth tlaa. Matarataly aarara liflaaitlaa af aaaal aa* aaalar aw*aaa ai aata* ahaa tha aalaala nr* rtarfa*. All ha* a all* **sraa af hraathlal ralaa. thar* aaa aa tail as hat traathlag aaa aaafhat dlfflaalt. laflaaaatlea ra*aaa* la taaaty faar haara aa* althls thraa ta faar Aaya traathlag ata atari? aaraal. lha aalaala ayyaara* la gaa* nail tin at tha aa* af tha taa tag ahaaraatlaa rrl*. MOMS 03i