Document jyLJJe3GqKBDJ5meg24p2eD42

M&3L& - 07/9 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST-SUBSTANCE_______________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. ( 1 Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203. Current information indicates it is a mixture of 1.34% PFOS, 35% diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fiuorochemicai component of the test sample. METHOD:_________________________________________________________ Method: Standard Methods (APHA), 1970 Edition Type: Acute static GLP: No Year completed: 1973 Species: Daphnia magna Supplier: In-house cultures maintained at Bionomics, Inc., Wareham, MA.. Analytical monitoring: Temperature, pH, conductivity and DO Exposure period: 48-hours Test organism age: 1 2 1 2 h r s . old Statistical method: TL50 (median tolerance limit) values calculated using a linear regression equation. Test conditions: Dilution water: Well water Dilution water chemistry: Alkalinity: 35 mg/L as CaC03 pH: 7.1 Lighting: Not given Stock and test solution preparation: Exposure concentrations prepared by direct addition of test substance. Exposure vessels: 250 mL beakers with 200 mL exposure solution Number of replicates: 4 Number of daphnids per replicate: 5 Number of concentrations: five plus a blank control Food: Food, consisting of a homogenization of starter trout food and Cerophyl in water, was added at rate of 0.5 mL/L after introduction of test substance. Water chemistry during the study: pH range (0-48 hours): 7.1 000546 Temperature range (0-48 hours): 21 1 C Dissolved oxygen range (0-48 hours): 5.3 - 8.9 mg/L RESULTS_________________________________________________________ Nominal concentrations: Bk control, 560, 87 0 ,1 0 0 0 ,1 8 0 0 , and 3200 mg/L. Element values: 48-hour EC50 = 1560 (1280 - 2140) mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ________________________________________ ___________ The test substance 48-hour EC50 was determined to be 1560 mg/L with a 95% confidence Interval of 1280 to 2140 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES _________________________________________________ Test was conducted by Bionomics, Inc., of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1973. OTHER___________________________________________________________ Last changed: 6/27/00. 000547 'W S I BIOASSAY REPORT SUBMITTED TO MINNESOTA MINING & MANUFACTURING COMPANY ST. PAUL, MINNESOTA fi Ii i ! ) I . .' .- ACUTE TOXICITY OF 3M SAMPLES (A AND B) TO THE WATER FLEA (Daphnia magna)AND SCUD (Gamraarus fasciatus) . I' i ` .K I `I Bionomics, Inc. 790 Main Street Wareham, Massachusetts December, 1973 0005<J METHODS AND MATERIALS These investigations were performed at the aquatic invertebrate toxicology laboratory of Bionomics, Inc., Wareham, Massachusetts. The susceptibility of the water flea, Daphnia magna and scud Gammarus fasciatus to 3M samples designated as sample numbers A and B (100% active) was evaluated under static conditions for a 48 hour experimental period. Results were, expressed as the median tolerance lit (TL^q ) , tb nominal concentration of the test compound in water causing 50 percent mortality. The TL^q values and 95% confidence intervals were calculated by converting the test concentration and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. iT ir L M Both Daphnia and Gammarus were obtained from laboratory cultures and judged to be in excellent condition. Procedures, for the static bioassays adhered to the protocol described by the 1970 edition of Standard Methods (APHA). Static Daphnia and Gammarus bioassays were conducted in 250 ml beakers with 200 ml of the experimental diluent. The diluent water was obtained from a well source and was used as such. The experimental chambers were maintained in a 000549 water bath at 21 * 1C. Five 12 * 12 hour old Daphnia were introduced into each experimental beaker and five fourth instar Gammarids were introduced into each experimental chamber for the Daphnia and Gammarus bioassays, respectively. In the Daphnia bioassays, food was added at a rate of 0.5 ml/liter after the introduction of the compound to the test vessels. Bioassays were conducted using four replicates of each concentration for both compounds (A and B) for both species. The pH of the standard diluent was 7.1 and the methyl orange alkalinity was 35 ppm as CaCO^. Dissolved oxygen values for the various test vessels for both compounds ranged from 8.9 initially to 5.3 mg/1 at the end of the test. ^Food was prepared by homogenizing 5 g of starter trout food and 1 g of Cero-phyl in 1Q0 ml of water. 00055Q, RESULTS 'I The predicted 48 hour TL^q (i.e. that concentration of active compound that allows 50 percent survival to the experimental animals) and 95% confidence intervals for Gammarus and Daphnia exposed to compounds A and B are presented in Table 1. A summary of the observed mortality of Daphnia and Gammarus exposed to various, concentratibns of compound A is given in Table 2. The observed mortality of Daphnia and Gammarus exposed' to experimental concentrations of compound B is given in Table 3. v j 0G053& SUBMITTED BY: PREPARED BY ) APPROVED BY: J /db Bionomics, Inc. 790 Main Street Wareham, Mass. December, 1973 S. Krogh Derr, Ph.D. Invertebrate Toxicologist if Kenneth J. Macek, Ph.D. 'V' Director 000553 Table 1 - Acute toxicity of 3 M compounds A and B to the water a 'b flea (Daphnia magna) and scud (Gammarus fasciatus.) exposed for a 48 hour experimental period. Species 48 hour TL^Q-mg/liter Compound A Compound B Zo'y Daphnia magna 31.1 (23.3-41.5)c Gammarus fasciatus 15.4 (12.8-20.1) 1560.0 (1280.0-2140.( 1130.0 (843.0-1310.0} aBioassay conducted at 21 * 1C, Daphnia, 12 - 12 hours old at initiation of tests. bBioassays*> conducted at 21oC 4-" 1C, 'Gammarus, fourth inis*'itar at initiation of tests. c95% confidence intervals. i1 0 0 0 5 5 3 Table 2 - Concentrations tested and corresponding observed percent mortalities for the water flea, (Daphnia magna) , and such (Gammarus fasciatus) exposed to 3M compound A for a 48 hour experimental period. Species _ Water Flea (Daphnia magna) Concentration mg/1 42.0 32.0 - 24.0. 12.0 10.0 7.5 Control % Mortality Observed 48 hour 100 60 0 0 0 o 0 Scud 32.0 (Gammarus fasciatus) 24.0 18.0 12.0 7.5 . Control 100 70 60 50 0 0 Table 3 - Concentrations tested and corresponding observed percent mortalities for the water flea (Daphnia magna) and scud (Gammarus fasciatus) exposed to 3M compound B for a 48 hour experimental period. Species Water flea (Daphnia magna) Concentration mg/1 3200.0 1800.0. 1000.0 870.0 560 .0 Control % Mortality Observed 48 hour 100 45 5 0 0 0 Scud (Gammarus fasciatus) 3200.0 1800 .0 1000.0 870.0 560.0 Control 100 90. 45^ 40 0 0 ;v ___ 000555