Document jBMj8ddaQBdG73kjMpaykYONy

AR226-2787 FOR D PONT USE ONLY E. I. du Pont de Nemours and Co., Inc. Haskell Laboratory! for Toxicology and Industrial Medicine Elkton Road, P. 0. Box 50, Newark, Delaware 19714 HASKELL LABORATORY REPORT NO. 234-84 Haskell Ilo. -- W , 787 ......... - - ` !-- -- - -- - -- SUBCHRONIC INHALATION TOXICITY OF, ft SUMMARY: G c a u g ^ f 10 male Cr1:CD* rats were exposed to either 0.31, i.4, or 3.0 mg/L o f j ^ H j I n air. Exposures were 6 hours/day, 5 days/week for 2 weeks. A control group was simultaneously exposed to air. At the end of the exposure period and after a 13-day recovery period, blood and urine samples were collected for clinical jsnalysis and rats were sacrificed for pathologi cal examination. No jvert clinical sign's or control rats and rats exposed to lain differences were noted between C11n1caf>atho1ogy measurements made at the end of the exposure and recovery periods showed no compound-related differences between control rats and rats exposed to 0.31 mg/l. Rats exposed to 1.4 and 3.0 mg/L had significantly increased seruin cholesterol concentrations after the exposure period but not after 13 days! recovery. Other differences were Interpreted to be within the range of biological variation. Gross patholog.cal examination following the exposure and recovery periods revealed no compound(-re1 ated changes In any rats. Histological changes were observed after the exposure Period in the livers of rats exposed to 1.4 and 3.0 mg/L. These changes consisted of an Increase 1n hepatocellu lar mitosis and hepatocellular hypertrophy in the centrilobular area. Thir teen days later, no c mpouhdj-related differences were observed. A comparison of organ land body weights between test and control rats showed no compound-related differences between control rats and rats exposed to 0.31 mg/L. Rats exposed to 1.4 and 3.0 mg/L had significantly increased liver weights and Uver/bodyiweight ratios after the exposure period. There were no significant differences In mean organ weights and organ/body weight ratios aftor 13 days recovery. lased on parameters evaluated In this study, a no-effect level for ras established at 0.31 mg/L. Company Sanitized. Does not contain TSCA CB - 1- I. INTRODUCTION: The purpose of this study w a s ^ ^ e t e r m l n e the effect on male rats of repeated sublethal exposure Exposure concentrations were bajsed on the results o f ^ T a n g e n n d l n g study where a 4-huur approximate liethal concentration was found to be 16 mg/L. II. PROCEDURES: A. Animals: Male, 7-Week old Cr1:CD* rats were received from Charles River Breeding Laboratories, Kingston, New York. Rats were housed in pairs in 8" x 8 M x 14" stainless steel, wire-mesh cages. Each rat was assigned a| unique identification number which was recorded on a card affixed Ito his cage. Purina Certified Rodent Chow* #5002 and water were avalllable ad libitum. Rats were weighed and observed for general suitability for approximately 1 week prior to testing. i B Test Material: Purity: Contaminants: Synonyms : Other Codes: CAS Registry No Submitted by: Jackson Laboratory C. Exposure Protocol:; Groups of 10 rats, (weighing 216-243 grams) were exposed w h o l e k b o d ^ o design concentrations of either 0.30, 1.5 or 3.0 mg/L o f U f f g g l A control group (male rats weighing 221-234 grins) was'exposea simultaneously to air only. Exposure was 6 hours/day, 5 days/week for 2 weeks. Rats were weighed and observed dally (weekends excluded) through the exposure period and for 13 days post exposure. I. D. Genecatlon: Gaseous atmospheres were generated by syringe-driving f l ^ P p n the surlface of an Instatherm* flask. Flasks used for I t n e T o w and the Intermediate levels were unheated; that used for the high level was; heated to 30C. Dilution air was passed through the flasks to carry the gaseous test material from the flasks to the chambers. ; E. ___ yt Samples of chamber atmosphere were collected at 30- minute Intervals by a gas-tight syringe. Samples were analyzed using a Hewlett-Packard 5790 gas chromatograph equipped with a flame Ionization detector. Samples were chromatographed Isothermally at 1S5C on a 6' x 2 mm I.D. glass column packed with 80/100 mesh Poropaik*Q. Concentrations were determined by r c .-occn^5 ` 2c o*m P aanVn y S ^ ed-0 0 " ` comparison with a standard curve* Standards were prepared by quantitative dilution of the test material in calibrated gas bottles. Chamber oxygen levels were monitored w U h a Biomarine* Model 225 Oxygen Analyzer. Humidity was measured with a hygrometer, and temperature > thermometer. F. C ';ni cal Measoi nents: Overnight (16 hour) urine specimens were collected from each rat 3 days prior to the first exposure, after the 9th exposure, artid after the 12th recovery day. Analysis included quantitative measures of volume, osmolality and pH, and semi quantitative te$ts for occult blood, sugar, protein, bilirubin, urobilinogen, and ketones. The appearance (color and transparency) was recorded and the sediment from each specimen was microscopically examined. Blood samples were taken from the tails of all rats 3 days prior to exposure and after the 10th exposure, and from 5 rats per group after a 13-day recovery period. Blood analysis Included measurement of erythrocyte, platelet and leukocyte counts; relative numbers of neutrophils, lymphocytes, |inoph1Is, monocytes and basophils; hemoglobin concentration; and mean corpuscular volume. Absolute numbers of various types of leukocytes were calculated from the relative leukocyte data. Hematocrit, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentrations were calculated form the;erythrocytic data. The serum activities of alkallne^hosphatas, alanine aminotransferase, aspartate aminotransferase ana concentrations of urea nitrogen, creatinine, total protein and cholesterol were also measured. G. Pathology: Five rats from each group were sacrificed after the 10th exposure, and ifmlfiing rats after 13 days recovery, for gross and h1stopatho!og1cl examination. Organs and tissues examined were the adrenal and thyroid glands, esophagus, stomach, duodenum, pancreas, jejunun, ileum, cecum, colon, heart, U v e r , nasal cavities, spleen, sternebrae with bone marrow, mediastinal lymph nodes, eyes, brain, trachea, lungs, kidneys, testes and epididymides. H. Organ and Bouy Weiglit Analysts: At each sacrifice, mean organ weights and organ to body weight ratios were determined for the heart, U v e r , lungs, kidneys, spleen, testes, and thymus (Appendices I and if). III. RESULTS: Chamber temperature ranged from 27-34C and chamber oxygen Tevel was maintained at 21%. Relative humidity ranged from 45-59%. DOS' Sanrt'ze - 3 ;omPan f % A. Exposure Data: Exp. # Low (Design * 0 .30 m?/L)j Mean S.u. ftanw Intermediate (Design 1.5 mg/L) Mean 5.D. Range High (Design * 3.0 mg/L) Mean 5.0. Range 1 0.45 0.22 .001-0.79 1.4 0.21 0.86-1.6 2.7 0.24 2.2-3.0 2 0.29 0.065 0.20-0.3? 1.2 0.25 0.85-1.6 3.4 0.13 3.2-3.6 3 0.28 0.043 0,26-0.41 1.4 0.18 0.88-1.6 3.0 0.41 1.8-3.3 4 0.29 0.050 0.23-0.3^ 1.5 0.11 1.4 -1.7 3.0 0.35 2.3-3.3 5 0.30 0.052 0.26-0.41 1.5 0.17 1.1 -1.6 3.1 0.31 2.7-3.5 6 0.34 0.059 0.26-0.4? 1.7 0.21 1.5 -2.1 3.5 0.40 3.0-4.2 7 0.28 0.037 0.23-0.35 1.3 0.18 1.0 -1.6 2.7 0.34 1.9-3.2 8 0.30 0.029 0.26-0.3^ 1.4 0.050 1.3 -1.5 2.8 0.66 2.6-3.2 9 0.30 0.023 0.24-0.32 1.5 0,057 1.4 -1.6 3.0 0.27 2.4-3.4 10 0.30 0.032 0.23-0.35 1.4 0.058 1.3 -1.5 3.0 0.11 2.8-3.1 Over all* 0.31 0.0?7 .001-0.7? 1.4 0.21 0.85-2.1 3.0 0.44 1.8-4.2 * Mean and Standard Deviation of individual samples from all exposures. t !.. X l i n L c al Observations: Clinical observations of rats exposed to I H H H r^e r e 1ndistig_ushable from those of controls. A few rats ^ fr.o...m... each group exh.i..b.i.t.e.d slight sporadic weight loss. Statistical comparisons of body weights showed no significant differences at any time throughout the study (see attached Growth Curve). C. Clinical M h o l o a y i Statistical analysis of the clinical chemical and hematological data Indicate that rats exposed to 1.4 and 3.0 mg/L had significantly Increased serian Cholesterol concentrations and decreased serum creatinine concentrations and platelet counts. These two groups excreted more ufdbllihpgen In the urine. Rats exposed to 3.0 mg/l[ also had decreases In serum urea nitrogen. Increases 1n serum;itotal protein, and excreted more alkaline urine. Following a 13-day recovery period, rats exposed to 1.4 and 3.0 mg/L had decreased hemoglobin concentrations. Rats exposed to 1.4 mg/L had Increased serum aspartate aminotransferase activities. Rats exposed to 3.0 mg/L had decreased absolute numbers of monocytes. 4Cow? TSCAel ed.Dcss noi co n ia i The dose-related Increases 1n serum cholesterol are Interpreted to be treatment related. The other changes are within the range of expected biological variation. The 0.31 mg/L concentration o f ^ p j w a s Interpreted to be a no-effect dose for the hepitologlc and clinical chemical parameters measured under the conditions of this study. D. Pathology: No Compound-related effects were detected at necropsy. Histologically, compound-related changes were found after the exposure period 1n the 1Ivers of rats exposed to 1.4 and 3.0 mg/L. Specifically, there was an Increase 1n hepatocellular mitosis, together with hepatocellular hypertrophy 1n the centrllobular area. Recovery was complete for both groups by the 13th day of recovery. E. O r o a n a n O Body Weight Analysis: Mean body weights of rats exposed t o U ^ P j r e r e simitar to tnoSe of controls throughout the study. After to exposures, rats exposed to 0.31 mg/L had significantly Increased thymus weights and thymus/body weight ratios. These changes are considered Incidental because they are absent In higher dose groups. Rats exposed to 1.4 and 3.0 mg/L had significantly increased liver weights and U v e r /body weight ratios. There were no statistically significant differences In organ weights between test rats and controls after 13 days of recovery. IV. CONCLUSION^ ^ t e i e d on parameters evaluated in this study, a no-effect level f o r ' M g f u s established at 0.31 mg/L. Liver effects were observed at 1.4 and 3.0 mg/L. Natarese, Catherine C. and Raymond N. Everett, "Clinical Patholgy Report No. 13 83," MR-4588-001, H-14,787, August 12, 1983. Stula, Edwin F. and William C. Krauss, "Pathology Report No. 38-83," MR-4588. H-14,787, June 2, 1983. % I Work anid Report by; *X Tnornas JA. Kegelman Technician Snervi sed by: Pal T. Turner Dale T. Turner Technician af ,rh iRayanneL. Ferenti J Toxicologist v'-' m 'f'x Approved by: TAK/DTT:sg1:3.18 Date Issued: June 27, 1984 jj/^/833/31/83 Haskell Lab. Report Number of pages In this repoHt: 13 >*kSraid L. Kennedy,'Jr. Section Supervisor/ Acute Investigations .,,,,,or-or."CACS C o mpany S3nfze*'^ -6- V"c M M te n rnnaAl I] TEST DAYS M ------- rfiffp r m a m .. 'a(l, |t|S ,t tlllIARY DATA PERIOD GROUP CONTROL 0.3 MG/L t . MG/L 3.0 MG/L F RATIO(I) LSD< 2) DUNNETT <3> W M S (4) 8R00P - l)CONTROL ).3 MG/L L.5 MG/L 5,0 MG/L F RATIO(l) LSD< 2) DUNNETT(3 ) WMS< 4) p.i.,.;.y;>./i-T 4. ---- -- -- . . . . . . u s i 227^01 .5000 ' 'T ? .% $ % ! y . . " * ; 2 2 * - .\9 r u....... >5.4000 >2.3000 2 2 $ . 90.0 23i#'600I,Vt42:JS9.8000 249.0000 254.0000 252.3000 249.6000 iim A .4 * ff 4 9 .0 8 3 3 M%>bf sfe; 0.744 tW 3 m $ j0 ^ M 8 . 3 1 8 6 S'.'il776* ' # i i 0 . 1 2 1 6 54 .`8 056' .^83.9611 0.650 8.3350 10.1415 84.2917 ITER.VALS- JM-, T?iSS^OPBSiAYS i:':i ; '" '^ ' % -, 'ail 276. :i:ISsSifSoeiig. .P-- io< --------------- ifs? .284.1000,. ' & 0 .6000 /78.6;0: _ 286."9099; J5!%93.1000 7 4.84 '-K 2 8 1 . 3 0 0 f :; \ 6 . 4 0 0 0 l/o iif 1*150 L l.3979 Jiif^lwiSWil 1.8025 13:J S t" 4.3607 157.6000 ;16 tb 9.0167 B- il. 296.9000 303.90*0 298.6000' 294.1000 0.688 12.5677 1 5 .2 9 1 7 1 9 1 ,6 4 1 7 mi.:; &ii a wa 2 54.2000 2 -6 0 .0 .S # ;:%. i p . 9 0 0 0 ' 254.9090 0 .* 9 3 1 0 .9 0 6 3 97.4833 3 0 2 .0 0 0 0 0.061, 1 4 .lti7 i' 18. 2* 8 .' C D ` RATIO OF AMONG- TO WJTtfffNi^ , P , - V R l 6 ^ 0 ^ l ) N E - F ACTOR ANALYSIS OF VARlRflJ* ` - 2t . . | :2) LEAS S & N I f i W LUFf^l|j|[E' If-1__C_A_NT (ALPHA=OniO.Siiii-|.. .R.it R_j;Tiili_O_ yny two Me a ns di f f e r i n g by ItTH A Fh I-SE POSITIVE <i " " s i g n i f i c a n t l y ',i"'`H,"lJ' ` ::*) DUNNETT ES--ANY TREAT* THAN THE feUWMEtt -Sf#T JSfrMM l JITH A VARIABLE -Wi fE FROM THE CONTROL ME*#?fY MO R E %ag . , ,MJM z n t H t FROM THE CNT k #1*11 c *~mV 3R RATE OF 0.05. ' ;4 > WITKIN^GRUP MAM f&sm ;- SIGNIFICANTLY DIFFERENT SIGNIFICANTLY DIFFERENT <P<Oi.OS> FROM GROUP BY LSD. GROUP BY DUNNETT TEST AND L80, t.-rvfeed- Does I n^i c oniain t s c a csi 5 ^ B p a R ? **Xl .... r % G IDY WE IGHT (ORAMR),- $ttffMARY 14787 - OBSERVATION PERIOD GROUP INTjERVALS I Tf|T ^ 9 8 15. mug- 17. CONTROL 0.3 HQ/L 1.5 MQ/L 3.0 MG/L F RATI O ! 1) L S D ( 2) D U N N E T T (3) U M S (4) 315.4000 321.0000 317*6000 308.4000 0.441 2 4 0438 29.8293 321.6000 314.8000 326.4000 321 .'OjpOo., 309.600b 322.0000 32,9.8000 313.4000 310.6000 0.843 23*8741 , 29.6188 317.0750 - . ` r' 1.082 a p.0261 8.5667 v2-tw9*4 . 9 5 0 0 18. 320.6000 329.8000 330.8000 313.8000 1.199 22.0857 27.4000 271.3500 324.0000 336.400^ 322.2000' 22.9420 292.8000 ,,>>---G-R-O-UP--- ----:ONTROL >.3 HG/L t.5 HG/L (.0 HG/L F R A T I O ! 1) L S D (2) D U N N E T T ! 3) UHS< 4) INTERVALS IN t e s t DAYS 22, 354.0000 369.8000 356.2000 348*8000 0.850 29.1253 36.1336 471.9001 ;.v \. vf_ iS'/CK v 24, 359. IM&i 0000 .4000 3i 4f fi .4000 2000 ........ . ' 111 1 .302; ,v 1.067 .27.7||9,,, 28.9489 34,42lt ui 3 . 9147 429.3250' 466.2000 25, 358.4000 385*4000 363.0000 359.4000 1.623 29.9379 37.1417 498.6001 1) RATIO OF AMONG- TO UI CHIN-GROUP VARIAji$ji^GflE-FACTOR ANALYSIS OF VARIANCE 2) NY TWO HEANS D I F F E R I N G B Y M Q A E THAN ITH A FALSE POSITIVE (ALPHA) ON IF ICANTL Y D IFFERENT ' - ^ T O M ^ t e p p t K * r s ? .3) DUNNETT TEST-- ANY TREATMENT. MEAN THE CONTROL MEAN RY n M t 4) WITHIN-g r o UP MEAN SQUARE. | 'f SIGNIFICANTLY DIFFERENT (PiO.oJs) FROH CONtRt. ROUP BY LSD. SIGNIFICANTLY DIFFERENT (P<0.03> FROH CONTROL GROUP BY DUNNETT TEST AND LSD. |S not contain TSCACBi Company. Sanitized.DoS GROUP im a fyORGAN g BQUY UEj IBHTS 4787 <GRANS) - AFTER ~ AftpOLUTE DATA TEN EXPOSURES FINAL Wi /mHitlis LUNGS CONTROL 0.3 MG/L 1.5 MG /L 3.0 MG/L <304:. 0 3 0 2 . 6( 305.6 3 0 1 . 4( -'il:- i , momIiW:"-. --v;.*.`P'-r-.. :<%$ i U ms fa :;j 0.00) 0.61) 0.77) 0.97) 1.521 1*53 1 .50( 1.38( 0*00) 0.00) 0.88) 0.09) TEST - HOMOGENEITY TEST - TREND 1.0' 0 .V -.p :0tft 0.21 0.09 GROUP LjIV E R ' SP$EEN KIDNEY CONTROL 0.3 MG/L 1.5 MG/L 3.0 MG/L 11.441 12.49 13.83 14.Q24 Q .00) ' 0..05 ,, 0.00) 0 Y20) -. 0.53) 0 .02) t 0 .01)4 0*504 0.24) 0.62 ,, 0.72) TEST - HOMOGENEITY 0.04, 10. ^ TEST - TREND 0.01 ( **.O*.'Ai GROUP IS t h y m u s 2.59 2.64 2.59 2.74 0*080 0*77) 0*98) 0.45) 0.80 0.52 CONTROL 0.3 MG/L 1.5 MG/L 3.0 MG/L 2.72 r 6 .00) 2.78i fi.pl> 2.79 0 *fQ) 2.SSX,- -io .50) 0.62 ' 0.00) 0*7? 0.00)4 *?< - 0.13) 0 '/o'9< 0.15) TEST - HOMOGENEITY TEST - TREND 0.75 .5 , 0.04 ; ::/'V'AT-v 'J.':* s ~ = ~ -- - -- - -- -- -- - = -- = -- -- - -- = = S * ---g Values in parentheses * P y'AL'JE, OF STUi)iC Jf!'M S T COMPARISON ' OF 'm lATftl^t TROL MEAN, HOMOGENEITY - P VALUE (jJF^F T I P T O E H H E t H t R G&jfjp MEANS ARE EQUAL. TREND - P VALUE OF F TEST OF iMtTflfeR'THiR^S'ftsE-RELATED CHANGE IN GROUP WEANS t .. ;ssstse - 10 - . Qqrnpani' S a w lnTSCiLCBl d C 03s M t " Ki GROUP if5! * B0D* WEIGHT 4GRAMS) " ABSOLUTE DATA rl H - 114477A077 -r a8ifA^Tlct^>rfDi;'A F ^r f :'|*|-_D_A_Y__O_B^S__E_R_V__A_TION FINAL tioilv" . LUNGS iiiiMiiaii CONTROL 0.3 MG/L l.S KG/L 3.0 HG/L 359*4( 385.4( 363.0( 3 5 9 . 4< TEST - HOMOGENEITY 0.2 TEST - TREND 0.7 0i. 0 ) 0.1) rysP9) 1: 1.4004/ ^0.000) l.,270irV 0 . 1 2 1 ) : - : & 4 W ^ h|0.915> GROUP LIVER '0 iSi-ifs ^SRLEN CONTROL 0.3 HG/L 1.5 HG/L 3.0 HG/L 14.7404 15.2324 15.1644 13*2404 i>: TEST - HOMOGENEITY TEST - TRFND ---------------ijgfpijg 0J60i|pS0.OOO> .o^fc^<j>.3i6> llte^>*000> mmm 1 707.4 1.7344 1.7744 1.6684 0.000) 0.749) 0.445) 0.463) 0.467 0.777 KIDNEY 2.9924 3.1764 3.1244 3.12%: 0.000) 0^347) 0*491) 0.51D) 0.796 0.586 GROUP CONTROL 0.3 HG/L 1.5 HG/L 3.0 HG/L 2.6964 3.0004 3.0604 2.9164 TEST - HOMOGENEITY TEST - TREND >a9S38KaBaBS8ss8Baap9cax8 Value* in parentheses - OiOfO) 0.05) 0.0221 oap> __ _ ~0-v476^^.000) YO.7|0p|i-,^>.689 ) Oi704;4 0.228) . .-.if* COMPARISON HOMOGENEITY - P UALUE OF p' t i i t e ^ ^ ^ ^ H E . a 8 ARE EQUAL. TREND - P VALUE OF F TEST OF M O T H E R M E * M oiE-RELATED CHANGE IN GROUP MEANS. | 4;?:? iW tsc* cav po5 il0,`c o n ^ n - 11 "r -3 41 UEI3HTS(GRAHS) - RELATIVE DATA H# 14787 - AFTER TEN EXPOSURES GROUP fWEART CONTROL 0.3 MG/L 1.5 MG/L 3.0 MG/L TEST - HOMOGENEITY TEST - TREND 0.3 Ait': 0 35< 0.35( 0,35 < 0.70 0. m O'. 99): J 6y?9 " ". 0 . 5 0 & ''''SSlS^li.O.OO) 0.5|if 0.75) 0.47( 0.75) 0.43T" Y 0.06) vi: >. - m # "o|ii LIVER 3.76< 4.11 ( 4.521 4.65< 6.09) 0.071 0*00)9 0.00) 0.00 0.00 GROUP S'FtEE Mi KIDNEY mm TESTIS CONTROL 0.3 MG/L 1.5 MG/L 3.0 MG/L TEST r HOMOGENEITY TEST - TREND GROUP 0.21 < 0.20 < o0.;50:0!1>.))*.--.iC'.1. 0 .1 8 < \r-Oi 17) 0.20 < 6;7).. 0.55 9% 52 YO'*V*A. i-r'SX- r'Y' THY MOW ? # o.ja|i( ^ o.oo) 0*^8) O.ESiip" Y 0.92) 0.27) -ivSijic .. '.i; 0.901 0.93T 0.91 < 0.85 ( 0*00) 0.77) 0.86) 0.62) 0.83 0.61 CONTROL 0.3 MG/L 1.5 MG/L 3.0 MG/L 0.20 < 0.26< 0.?3( 0.23< m. 0 Wi0 0,0)9 "`t i 4); ' 0 12) : j-iSI TEST - HOMOGENEITY 0.03 TEST - TREND 0.33 If =si= = = = = r " = = = * * = -- : z i : S E = s x s i : s s s - Values in parentheses - - M f C O M P A R I S O N ' f R E M ^ T MEAliPi^^NTROL MEAN. . ,.- HOMOGENEITY - P VALUE Of F_ MEANS ARE EQUAL. TREND - P VALUE OF F TEST OF CHANGE IN GROUP MEfcfaS. " ' ` THE l E :^ S S s E - R E L A T E D ,::ii"-'~-STr"-ii'-r.lfJ`'-lji"Rl: ilai[Lt s < * c * 3 noicon S a w^ oea GROUP e CONTROL 0.3 MG/L 1.5 HG/L 3.0 HG/L t BODY WEIGHT (GRAMS) - W m RELATIVE DATA iH-14787 - UACRIFICiD AFTER^3-DAY OBSERVATION HEART :JS k?i JNGB LIVER 0.3304 0.33K 0.324< 0*3324 -- - *m4.xlttllS!10. o> 0. 921) 0.8997 > .;s5 0.000) 0.417) 0.665) C" 0.680) 4.1144 3.9474 4.1704 4.2444 0.0009 0.35$) 0.751) 0.465 TEST - HOMOGENEITY 0.9A4 0.395 TEST - TREND 0*9*7 0.280 GROUP SPLEEN ;t I I ^ n e y TESTIS CONTROL 0.3 MG/L 1.5 MG/L 3.0, HG/L 0 1914- '''-O, 0 .1 9 6 4 0 7997 rr 0.814) 0 . 19< G 918) 0.592) 0 . 1 9'3V3(r'*'' 9 `^ f e S l M f t 0.490) TEST - HOMOGENEITY 0 .i9 9 S . TEST - TREND 0 .9 4 9 0.7544 0.7954 o.84S< 0.8I^< 0.000) 0.580) 0.294 ; 0.412 0.189 GROUP THYMUS CONTROL 0.3 MG/L 1.5 MG/L 3.0 MG/L TEST - HOMOGENEITY 0.1884 0.1854 0.2104 0 c22a ( 0, -.971")?' ' 0. 'S*-- *" 0 Ml-)' 0*297 '_-*V,(vib* H-fife TEST - TREND 0.085 S& '. SS 8SI SS SSSSSS sasassa Valu* in prnth*ss - Jiii '->VS-. ::rlu+-f HOMOGENEITY - P VALUE OF j B S COMPARISON :"^ W ^ NTRGL m e a n . G FUHETHEft|GROGP MEANS ARE EQUAL. TRENO - P CH VA AN LU GE E OF IN F GR O UTjPESJMftPIitGNF-SJ.Wtf Er T 'R: - ^ f ` c m s E ^ R E L A T E D m jSC^ 09ilGOn*a'in , ^ - A . oSS