Document gamDRpGDbjqnZpwDE5xpozBeV

ARIX-OSfl ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: Perfluorooctylsulfonate, didecyldimethylammonium salt; may also be referred to as Fluoroalkyl ammonium derivative. [1Decaminium, N-decyl-N,N-dimethyl-, salt with 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-1octanesulfonic acid (1:1), CAS # 251099-16-8] Remarks: The 3M production lot number was Lot 1. The test sample is L-14394 referred to by the test laboratory as P3025. The sample was labeled F-11615, Lot 1. The test sample is a mixture of the test substance in water (approximately 30-40% test substance, 60-70% water, and 0-5% of residual perfluorochemicals). All values reported relate to this mixture. The test sample appears to be a 2-phase dispersion (clear liquid with opaque solid) which rapidly separates after agitation. No calculations were made to adjust for the actual concentration of the test substance in the test sample. METHOD Method: OECD 209 Test type: Static acute GLP: No Year completed: 1997 Analytical monitoring: Nominal concentrations. pH, temperature and dissolved oxygen. Exposure period: 30-minutes and 3-hours Statistical methods: not applicable Test organism source: Activated sludge mixed liquor collected from Metro Wastewater Treatment Plant, St Paul, MN Inoculum mixed liquor suspended solids: 4.38 g/L Inoculum holding: None - used the day it was obtained Test conditions Dilution water: Millipore Milli-Q water Synthetic Sewage: Per OECD method Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10 mL of 1N NaOH, diluted to 30 mL with MilliQ water, then brought to the point of incipient precipitation with 1N 004238 H2SO4, and diluted to 1 L with Milli-Q water. The pH of the reference solution was measured to be 7.3. The test solutions were created by direct addition of the test substance to the test vessels. After test substance addition, the vessels were stirred with a Pyrex pipette for approximately one minute. A white precipitate was seen on the bottom of the vessels after stirring. The particles were suspended during aeration. Vigorous shaking was done before removing each sample aliquot for testing. Test vessels: 1200 ml_ Pyrex FleakersTM containing 500 mL test solution Number of concentrations: 3 plus one reference control and two blank controls Temperature: 19-21C Total Suspended Solids and pH for sludge on day of testing: 4380 mg/L and 7.87 respectively. Element basis: respiration inhibition as determined by oxygen consumption Method Remarks: Test temperatures were not controlled. The respiration rates were normalized to 20C for the inhibition calculations. RESULTS Nominal concentrations: Blank control, reference substance control (10 mg/L), 100, 500, and duplicated 1,000 mg/L test material solutions Element Values: 30-minute EC50 = >1,000 mg/L 3-hour EC50 = >1,000 mg/L Element value based on nominal concentrations Analytical Methodology: Analysis of DO concentrations in all test solutions were performed using a dissolved oxygen meter. Control response: Satisfactory Remarks: No oxygen inhibition seen in the 1,000 mg/L exposure concentration relative to the control in the 3-hour exposure. CONCLUSIONS C042S9 The test substance exhibited only a slight inhibitory effect (<20%) upon respiration at a nominal test substance concentration of 1,000 mg/L at the 30-minute exposure and no inhibitory effect at the 3-hour exposure. The EC50 (respiration inhibition) is therefore greater than the highest exposure concentration, 1,000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request P3025 OTHER Last changed: 5/25/00 004290 3M ENVIRONMENTAL LABORATORY INHIBITORY EFFECTS OF P3025 ON ACTIVATED SLUDGE RESPIRATION OECD Activated Sludge Respiration Inhibition Test, # 209 STUDY COMPLETED: January 21,1997 FINAL REPORT COMPLETED: May 12,1997 Env. Lab Request Number P3025 Prepared by: Name: Title: Laboratory: Address: Susan A. Beach Advanced Environmental Biologist 3M Environmental Laboratory Building 2-3E-09 935 Bush Avenue St Paul, MN 55144 Activated Sludge Respiration Inhibition Page 1 May 12, 1997 SUMMARY The purpose of this study was to evaluate the inhibitory effect of P3025 on aerobic sludge systems in accordance with the "Activated Sludge Respiration Inhibition Test", OECD No. 209. The median effective concentration, 3hour EC50 was reported as > 1000 mg/L. INTRODUCTION Toxicity tests with aerobic sludge provide a rapid screening method and are reliable predictors of the inhibitory effects of potential contaminants on the operation of activated sludge treatment systems. This toxicity study measured the respiration rate of activated sludge obtained from a municipal wastewater treatment system and followed decreases in the rate of oxygen consumption over a relatively short period (30minutes to 3-hours) in the presence of different concentrations of P3025. last-Substance: Appearance Aqueous Solubility MATERIALS AND METHODS P3025, date 10/7/96, Lot 1 Two-phase dispersion (clear liquid with opaque solid) Apparently not completely soluble in water at tested concentrations. Available Physicochemical Properties: Date Received Storage Test Solutions Reported values Boiling Point: Vapor Pressure: Vapor Density: Evaporation Rate: Solubility in water: Specific Gravity: Percent Volatile: pH: 100C ca. 18 mm Hg @ 20C <1 <1 Insoluble ca. 1.2 60 - 70% 6-8 10/16/96 In the dark at ambient room temperature (22 2C) Test substance weighed out and added directly to test vessels. After test substance addition, vessels were stirred with a Pyrex pipette for approximately one minute. Test substance formed white particles which sank to the bottom of the FleakerTM. Particles were suspended during aeration. The reported values are based on nominal concentrations in the test media at the beginning of the microbial assay. Controls: Functional Control (Reference substance) 3,5-dichlorophenol (3,5-DCP). Aldrich red label; 97%. Lot 09615KG DQ. Received 11/18/96. c 04292 Activated Sludge Respiration Inhibition Page 2 May 12,1997 Contois ICanU. Blank control (untreated) Two controls containing inoculum and nutrient medium, but no test or reference chemicals, one at the start and the other at the end of the test series. Inosulum/Nutrient Mixture: Microbial Inoculum Nutrient Medium Activated sludge mixed liquor was obtained from the Metropolitan Wastewater Treatment Plant, St Paul, Minnesota on test day. The suspended solids level was 4.38 .072 g/L; initial pH 7.6. The sludge was continuously aerated after it was received in the laboratory. Synthetic sewage feed prepared on test day as outlined in OECD 209. Exposure: Test Date 1/13/97 Contact Time 30-minutes and 3-hours. Vessels 1200 mL Pyrex FleakersTM Test Solution Vol. 500 mL Starting Test Cone. Test Substance 100 500 1000 mg/L ( in duplicate at 30-min; 3-hour duplicate lost due to lab accident) Reference Substance 10 mg/L Mixed Liquor Suspended Solids Level in Test Media 1.75 g/L Dilution Water Millipore Milli-Q water Temperature 15.6 - 18.0C over the course of the study Lighting Ambient laboratory light Mixing and Aeration Test solutions were continually aerated with clean, oil-free air. Measurements Dissolved oxygen concentrations were measured after 30-minutes and 3-hours contact time. A sub-sample of test solution was quickly poured from the exposure vessel into a BOD bottle and dissolved oxygen concentrations were recorded using a dissolved oxygen meter every 30-seconds over a five-minute period. The sub-sample was stirred while recording oxygen. The sub-sample was returned to the main test solution after the 30-minute readings. C04293 Activated Sludge Respiration Inhibition Page 3 May 12, 1997 Procedure The test was conducted in general accordance with the "Activated Sludge Respiration Inhibition Test", OECD No. 209 (1). Values were reported to different levels of significance depending on the accuracy of the measuring devices involved in any one process. CALCULATIONS The reported values are based on nominal concentrations in the test media at the beginning of the test. The concentrations tested and the corresponding response data derived from the toxicity test are used to calculate, if possible, the medial effective concentration, EC50 and the 95% confidence interval by standard logistic regression methods (i.e., Probit Analysis, Trimmed Spearman-Karber). The EC50 is defined as the concentration estimated to inhibit the rate of respiration by 50% as compared to the controls, after 30-minutes and 3-hours of exposure to the test substance. RESULTS The percent reduction in respiration rate and the median effective concentration, EC50, obtained after 30minutes and 3-hours exposure to the test substance P3025 are summarized in Table 1. The percent reduction in respiration rate of the reference substance 3,5-Dichlorophenol is also reported in Table 1. The median effective concentration, 3-hour EC50 of P3025 to activated sludge was determined as >1000 mg/L. The 95% Confidence Interval could not be calculated. ACCEPTABILITY CRITERIA This toxicity test meets quality criteria provided in test guidelines: 1) The two blank control respiration rates were within 15 percent of each other after 3-hours exposure. The percent differences were 22.2 and 4.1 after 30-minutes and 3-hours of exposure, respectively. 2) The 3-hour percent inhibition induced by the reference substance, "3,5-DCP", was within the normal range seen with this mixed liquor in this laboratory (see Table 2). 3 ) The reported values were based on nominal concentrations in the test media at the beginning of the microbial assay. This test was conducted by Trudy K. Stiefenhoefer as requested by Pam Wolf of 3M SCD Division. REFERENCES 1. OECD (1984). Activated Sludge Respiration Inhibition Test, Test No. 209. In: Guidelines for Testing of Chemicals. Organization for Economic Cooperation and Development, Paris, France. 2. OECD. 1981. Principles of Good Laboratory Practice, Annex 2, C(81)30 (Final): 7-28. OECD Guidelines for Testing of Chemicals. Organization for Economic Cooperation and Development, Paris, France. C04294 Activated Sludge Respiration Inhibition Page 4 May 12, 1997 TABLE 1 SUMMARY OF TOXICITY OF P3025 TO ACTIVATED SLUDGE TEST CONC. mg/L 100 500 1000 1000 (dup) BERCENT INHIBITION IN RESPIRATION RATE 3Q-mi[L Exposure 3-hours Exposure 1.8 0 00 17 0 19 n a - lab accident EC50 >1000 >1000 95% Confidence Interval Not calculable Not calculable Statistical method used______________ Notapplied_______________________Notapplicable SUMMARY OF TOXICITY OF 3,5-DICHLOROPHENOL REFERENCE SUBSTANCE TO ACTIVATED SLUDGE TEST CONC. mg/L 10 PERCENT INHIBITION IN RESPIRATION RATE 3Q-min. Exposure 3-hours Exposure 36 54 004295 Activated Sludge Respiration Inhibition TABLE 2 Page 5 5/12/97 OECD 209 Results from Reference Toxicant Testing with 3,5-Dichlorophenol Test Concentration = 10 mg/L Percent Inhibition In Oxygen Uptake Rate Date Tested 11/27/95 1/25/96 2/26/96 3/4/96 3/7/96 3/11/96 4/4/96 4/8/96 4/17/96 4/25/96 5/16/96 8/14/96 8/19/96 8/26/96 8/28/96 9/5/96 9/9/96 9/16/96 9/30/96 10/2/96 10/9/96 10/14/96 10/23/96 10/29/96 10/31/96 11/4/96 11/6/96 11/18/96 11/25/96 12/2/96 1/6/97 1/8/97 1/13/97 1/21/97 30-min Exposure 38 36 32 27 34 34 29 25 36 37 55 50 54 46 52 48 69 40 36 44 48 30 28 45 42 55 53 30 52 28 40 49 36 37 3-hrs Exposure 58 53 58 65 57 58 58 60 56 66 52 73 68 52 57 49 37 58 45 54 61 46 55 59 48 54 64 42 59 50 47 41 54 56 MLSS*, g/L 5.5 7.2 5.2 2.4 3.4 3.7 5.9 3.9 4.7 3.2 3.6 3.3 3.0 4.1 4.0 3.1 4.2 3.7 3.8 3.9 4.1 3.8 3.0 4.1 3.6 4.4 4.7 4.3 5.2 5.6 3.3 3.8 4.4 4.4 MEAN Standard Deviation 41 10.3 ` Mixed liquor suspended solids 55 4.1 7.8 0.96 Metro Wastewater Treatment Plant, St Paul, MN 004296