Document gaQ5R8EvOjmZmaa0mV616bYeG

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:N-methylperfluorooctanseulfonamidoethanolm;ay also be referredtoas N-MEFOSE Alcohol,FC-790, or FM-3925. Inone toxicitsytudy on bluegilslunfish,referredtoas FC-3925. (iOctanesulfonamide,N-methyl-1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-N-(2-hydroxyethyClA)S-,# 24448-09-7) Remarks: Materialisan off-whitew,axy solid.Sample purityand lot number were notrecorded. METHOD Summary of 2 Studies Method: U.S. EPA 660/3-75-009,April,1975. Type: Staticacute GLP: No Year completed: 1978 Species:Lepomis macrochirus Supplier:Dale FaftigFishHatchery,Brady,Nebraska Analyticalmonitoring: Nominalconcentrationosnly. Temperature,pH and dissolvedoxygen recorded. Exposure period:96-hours Carrier:Acetone - 0.5mL per litetrestsolution Statisticamlethods: LC5ovalueswere notcalculatedbecause the highestconcentrationtestedinducedlessthan 50% mortality. Concentrationstestedexceed water solubiliotfythetestsubstance. .Testfishage: Not noted. Lengthand weight: Average length 4.0cm Average weight 0.5 g Loading: 0.63 g fish/L Pretreatment: None Test conditions: Dilutionwater: Carbon-filterewdellwater Dilutionwater chemistry: Hardness*: 236 mg/L as CaC03 DO: 8.9 mg/L COD*: <1 mg/L Alkalinity*: 221 mg/L as CaC03 pH: 7.9 Conductivity*: 420 pmhos/cm Total Solids*: 286 ing/L Values taken as averages from generallaboratorywater qualitytestingin 1978. Stock and testsolutionpreparation:Test solutionsprepared by dissolvinkgnown weightsofthetestsubstancein 8 mL acetone beforemixingwithdilutiownater. This resultedinan acetone concentrationof0.5mi per liteorfdilutiownater.The test substance was noted to precipitatoeutgraduallyover the course of the test.Itwas generallynoted thatmore precipitatfeormed inthe higherconcentrationtestsolutions. Concentrations dosing rate:Once Stabilityofthe testchemical solutions:Precipitatneoted Exposure vessels: 35x2Ox2Ocm (5gallon)glassaquaria containing16L oftestsolution. Number of replicates:one Number offishper replicate:twenty Number of concentrations:Fiveplusa solventcontroland a negativecontrol Water chemistry during the study: Dissolved oxygen range (24 - 96 hours): 5.7- 6.1 mg/L (controelxposure Test 1) 3.4- 5.8mg/L (controelxposureTest 2) 5.0- 6.0mg/L (100mg/L exposureTest 1) 3.4- 5.2mg/L (320mg/L exposureTest2) pH range (24 - 96 hours) 8.2 -8.2 (controelxposureTest 1) 7.8- 7.9 (controelxposure Test2) 7.8 - 7.9 (100mg/L exposureTest 1) 7.8 - 7.9 (320mg/L exposure Test2) Test temperature range (0- 96 hours): 17 - 200C Remarks: Test fishwere randomlyallocatetdo each test chamber within30 minutesfollowingtoxicantaddition.Mortality, temperature,dissolvedoxygen levelsand pH ofalltestsolutions were measured at 24-hourintervals A firssttudywas conducted atconcentrationstoo low to induce sufficienmtortalittyoallowthecalculatioonfan LC50. The study was repeatedathighertestsubstance concentrations.Inthe second study,fishmortalitwyas observed to be lowerthan inthe firssttudy.Thisnon-reproduciblemortalitwyas probablydue tothe insolublenatureofthe testsubstance. RESULTS Nominal concentrations: Test 1: One blankand one 0.5ml/Lsolventcontrol1,3,22, 36,60, 100 mg/L Test 2: One blankand one 0.5mi/Lsolventcontrol4, test concentrationsthatarenotlegibleon thetestsheet, and 180 mg/L and 320 mg/L testsolutions. Element value: 96-hour LC5o: Not calculatedforeithertest.Exceeds solubility. Test 1 Percent Mortality Nominal Test Concentration, mg/L 24-Hours Negative Control 0 Solvent Control 0 13 0 22 0 36 0 60 0 100 0 48-Hours 0 0 0 0 0 0 0 72-Hours 0 0 5 10 10 15 35 96-Hcxjrs 0 0 6 10 10 16 Test 2 Percent Modality Nominal Test Concentration, mg/L 24-Hours Negative Control 0 Solvent Control 5 22 5 36 0 60 0 100 0 180 5 320 5 48-Hours 0 10 5 0 0 5 10 5 72-Hours 96-Hours 5 5 15 15 1 6 5 0 0 0 15 5 5 15 5 CONCLUSIONS The N-methylFOSE alcohol96-hourLC5oforbluegilslunfishwas determined tobe greaterthan thetestsubstance solubililtiymit. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. These studies,whilewellconducted usingthe methodology acceptableattheirtime,lackanalyticadlata for determinationofthe testsubstance concentrationinthetestsolutionsand determinationofthe sample purity.Testingwas alsodone wellabove the solubilitlyimiotfthe testsubstance withthe aidofacetone toenhance ' solubilitTyh.e solventcontrolinthe second testwas greaterthan the allowablemaximum of 10%. Testingshould have been replicated. REFERENCES These studieswere conducted by the 3M Company, Environmental Laboratory,3M Lab Request number 4197, 1978. OTHER Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 Last changed: 5/17/00 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.DAPHNIDS) TEST SUBSTANCE Identity:N-methylperfluorooctanseulfonamidoethanolm;ay also be referredtoas N-MEFOSE Alcohol,FC-790, or FM-3925. (1Octanesulfonamide,N-methyl-1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-N-(2-hydroxyethyClA)S-,# 24448-09-7) Remarks: Materialisan off-whitew,axy solidS.ample purityand lot number were not recorded. METHOD Summary of 2 Studies Method: U.S. EPA 660/3-75-009,April,1975. Type: Staticacute GLP: No Year completed: 1978 Species: Daphnia magna Analyticalmonitoring:Nominal concentrationosnly.Temperature,pH and dissolvedoxygen recorded Statisticamlethods: ProbitanalysisC.oncentrationstestedexceed water solubilitoyf the testsubstance. Test daphnid source: Obtained from the Mogul Corporation. Test daphnid age at study InitiationN:eonates Test conditions: Dilutionwater:Carbon-filterewdellwater Dilutionwater chemistry: Hardness*: 236 mg/L as CaC03 DO: 8.9 mg/L COD*: <1 mg/L Alkalinity*: 221 mg/L as CaC03 pH: 8.7 Conductivity*: 420 pmhos/cm Total Solids*: 286 mg/L Values taken as averages from generallaboratorywater qualitytestingin 1978. Stock and testsolutionpreparation: Not noted inreport. ExposurevesselsT:estvesselwseredescribeadsjars.Each test vesselcontained100 mL oftestsolution. Number of replicates:one Number of daphnids per replicate:20 Water chemistry during the study: Dissolved oxygen (recordedat48 hours orat24 hours when alltestorganismswere dead): 7.8 mg/L (controelxposureTest 1) 7.6 mg/L (controelxposure Test 2) 7.5 mg/L (1000 mg/L exposure Test 1) 8.5mg/L (8700 mg/L exposure Test 2) pH (recordedat48 hours orat24 hours when alltest organisms were dead): 8.6 (controelxposure Test 1) 8.7 (controelxposureTest2) 8.2 (1000 mg/L exposure Test 1) 8.6 (8700 mg/L exposure Test 2) Test temperature range (0- 48hours: 19 - 20"C Element basis:mortalitaynd immobilization Remarks: A firssttudywas conducted atconcentrationtsoo low to induce sufficienmtortalittyoallowthecalculatioonfan LC50.. The studywas repeated at highertestsubstance concentrations. The testsubstance was noted to precipitatoeut graduallyover the course ofthe test.Itwas generallynoted thatmore precipitate formed inthe higherconcentratiotnestsolutions.. RESULTS Nominal concentrations: Test 1: Test 2: One blankand one solventcontrol,100, 180, 320, 560, and 1000 mg/L One blankand one solventcontrol6,50, 1000, 1550, 2400, 3700, 5600, 8700 mg/L. Element value: Test 1 48-hour EC50 (95% Cl):Not calculated. Test 2 48-hour EC5o (95% Cl):1352 (1194-1533)mg/L StatisticaElvaluation:The EC50 valueand 956/oconfidenceintervals were calculatedusing probitanalysis. TestI PercentMortality/immobilization NominalTest 24-Hours Concentration,mg/L 48-Hours Negafive Control 0 10 Solvent Control 0 0 100 0 5 180 0 0 320 0 10 560 0 0 1000 1 0 10 Test 2 Percent Mortality/immobilization Nominal Test Concentrationm,g/L 24-Hours 48-Hours Negabvo Control Solvent Control 650 1000 1550 2400 3700 5600 8700 0 5 0 0 0 0 10 20 60 60 100 100 100 100 1 100 100 100 CONCLUSIONS The testsample 48-hour EC5o forDaphnia magna was determinedto be greaterthan thetestsubstance solubililtiymit. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY 'ReliabilityK:limischranking3. These studieslackanalyticadlata for determinationofthe testsubstance concentrationinthetestsolutionsand determinationofthe sample purity.The testsshould have been replicated.There was no descriptioonfstocksolutionpreparation. Testingwas alsodone wellabove the solubililtiymiotfthetestsubstance withtheaidofacetone to enhance solubility. REFERENCES These studieswere conducted by the 3M Company, Environmental Laboratory,3M Lab Request Number 4197, 1978. OTHER Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 Last changed: 5/12/00 Form 6747-11-A TECHNICAL REPORT SUMMARY TO: TECHNICAL COMMT)NICATIONS CENTER - 201-2CN )(Irnporta-nItfreporitsprinteodn bothsidesofpopw, sendtumCapin to TCC.) oivision EnvironmentLaalboratory .rrwect Pate of Fluorochemical Project Aquatic Toxicity Studies: To D. L. Bacon . Author(s) A. N. Welter Notebook R*fwenco FU 3925 Dow Jan.8, 1979. Dept-Numbw 0535 ProjectNumbw 9970012600 018 ERWIDY" Numbutiel 09362 No, of Pow inciudins covershoot SECURITY loo Open icompony Confidential) 4bpeciaAluthorization) 3M CHEMICAL REGISTRY t44w ChemkoleRomrted 0 Ya [X No KEYWORDS: IS*locttorimfrom 3M Tboswnn. SuopW other voicotweerms.) EE & PC Div. (Environmental Lab) Toxicity (Aquatic) CURRENT OBJECTIVE' To determine the acute 96.hour LC value for bluegill sunfish (Lepanimaacrochiru)sand th1048 hour LC value for Daphniamagna exposed to varying concentratibns of FU 3925. REPORT ABSTRACT:(200-25w0ordsT)hisebmat informatiosdnistribubtyetdheTechnicCaolmmunicatioCnesntetro sior3tPAwstoCompanyR&D. ItisCompanyconfidentmiaatlerial. The limited water solubilityof FM 3925 prohibiteda definitive determination of the aquatic toxicity-of this test material. Both vertebrate and invertebrate LC50 values exceed the water solubility of FU 3025. ,c: R . A . Prokop D. Ricker R. L. Bohon M. T. Elnabarawy A. Mendel E. A. Reiner S. K. Welsh informationisson 2 CONCLUSION The limited water solubility of FM 3925 prohibited a definitive determination of the aquatic toxicity of this test material. Both vertebrate and invertebrate LC 50 values exceed the water solubility of FM 3925. INTRODUCTION The test substance FM 3925 is an off-white solid. Tests performed on this material evaluated its aquatic toxicity and the data obtained form the basis of this report. METHODS A., Aquatic Testing The test protocols utilized for these studies were modeled after that described by US/EPA (1975). B* Acclimation Procedure The bluegill sunfish (Lepomismacrochirue)were obtained from a private hatchery+. Stock fish were held in a fiberglass holding gank filled with carbon-filtered well water at 14-15 C. A daily photoperiod of 16 hours light and 8 hours dark with a 30-minute transition period was maintained throughout the acclimation and testing period. The fish were fed Tetra-Min daily, food being withheld 48 hours prior to and throughout the test period. Fish were acclimated for 14 days prior to testing. An acute short-term (96-hour) static bioassay was performed on FM 3925. Carbon-filtered well water of known composition was used as the diluent (Attachment 1). All glass aquaria (35x2Ox2O cm) containing 16 liters of diluent or diluent plus toxicant comprised a study chamber. Twenty bluegill sunfish, uniform in size, were tested at each chemical concentration. Test fish were randofhly allocated to each test chamber within 30 minutes following toxicant addition.0 Bluegill sunfish experiments were conducted at 20-21 C. Mortality, temperature, dissolved oxygen level and pH of all test solutions were measured at 24-hour intervals or until total mortality had occurred. Pw-. General observations relative to behavioral changes ,were similarly recorded when appropriate. These studies were performed in replicate. Organisms used in this study were considered to be generally healthy and free of disease. The Daphniamagna used in this study were obtained on April 19, 1978 from the Mogul Corp. Test protocol used was that described by USEPA (1975). + Dale Pattig Fish Hatchery, Brady, Nebraska 0 Commercial Fish food of known composition 3 RESULTS The bluegill sunfish Lepo@rtmsaoroohirue)used in this study had an average weight of 0.5 g and length of 4.0 ems. The loading factor for these studies was approximately 0.63 g/l tiiow the maximum recommended loading factor of 0.8 g@i Test-temperatures ranged from 18 - 260C, pH varied from 7.8 - 6.2 pH units and oxygen saturation at 96 hours in the individual experiments were 34 and 56%. In this replicated study LC values were not calculated since these values would 'exeggd the water solubility limits of FM 3925. (Attachment-2). Thg Daphniamagna study was conducted at a temperature of 19 C., pH range of -&-.2- 8.7 and a minimal 48 hour oxygen saturation of 79%. Projected LC values exceeded the water solubility of FM 3925. (Attach;gRt 3). The data reported herein are based on studies performed by M. T. Elnabaravy-and L. H. Pritchard. REFERENCES 1. US/EPA - Methods for Acute Toxicity Test with Fish Macro-invertebrates and Amphibrians, Ecological Research Series 660/3-75-009, 1975. FM iFFtibASSAY LA 7M .-%& lr lit-If ------------- ATTACIPTENT I 4! 0 117 a Lis 0 0 Imm 4W 110 OL -Cool INVIRMWWRAL ENGINEF-RINGEADMAIM AQMTIC IOXICITY DATA SMET Ar-"rACEME#R2r Tnn Test INOOORof& 17,(. Material Test orsmiss -P.JadAilo %\N-^!gr6 Avs. Vt. S- Avg. Siz*.@@cs Tost SolutionVol@--- 16 utenamak No. orsmism per do" TimeSt&rw DiluticaUtair Elft Started %1-31 Filter- Noll Loter CTOO .Pa"cowl"" Invostiptwjk&ljam"vw%ol Lc So (gst cmtidmco units) PH.-I..Oii NO. rgmis Cost-itro 7-0 %13 2c) -2ez 2.C> 24 Mrs, D.O. aR2#A 0 0 .0. 0 .7.4 (M-13 0 C-40 46 firs, risma D.O* 0 17-f 6.o 0 72.Hrs, aqlad DOOO 96 Hrs. A2ldead 1 7.t 16.tl -zs 0 29 C.1 2 60 0 7.t 60 0 7.8 -1 -7.9 too -zo 0 7.9 0 "7q. ir6. 1 -7.9 7.9 v Ton. co@ts: d, %,t-vm ecAmi@,*.AL att%re504t I"L&4) ONVIRONNENTALENGINEERINGTANUTORY AQMTIC IOXICIrt DATA &IBET A'-TTACHMER-" #2 Typo TostN Test 4% 1 Naterial Test*d EL L: Z@O@ Avg Wt.04nnm Avg. Size co Test Solution vol@ Tim Started Dilutionwater Glltn Tmk No. orpatow Date Started.h .a -.1 Filtor- Noll Water-(Tow# per d"o ato 0W 0 oloo AO Iotod. investigator-Q@ IC SO (95% Confidmeo L$Bits) NO. is 24 Hro it O:keA D.06 0 0110. 72 itm 1 0000 lirs. bLPI.Danddi 3i 14 IL .0.@ 12 I 4iS,61 -&o I lj,-Os).I%t o- 0 P7.93..0 (03 -o S-i -:s 7. a c") 10L.ti of I t @ Ilk-1 1 -,-7.7 ),I,qs4 3 3.. 7-t 7 @,l 1.41 o)c*Cid -7- ENVIRONW;NTAL ENGINEF.RING LOORATORY AQIJATIC TOXICITY DKTA MEET LAI i% A-NACMDU #3 Type Test Material Tested C@ Test Organisu'y Avg. Wt. Zl=amn Avg. Size co Test Solution VolumloovA momj@F No. Organism per do" Time Started Dilution water D%IM lavestiptorl Date Started Comieted 0 Filter - Well Water -(Tom0-Z ct 0.0.3.)_=. pH LC 50 (96%ConftdewoUnits) No. )r,.,. EgL P,4 C=tro,, 24 Un. 0.0, 49lirs, -MR-Raidail-m- P-Ono-. o,o.n ift, JOL Molba D.O. 96 lirs. D.O. in_ No.Doad rnal @O lo (3 U P7. g@60 100'0 4va. Tome. Co@ ts: %( (k t LADI e, Ny ENVIRONMMAL ENGINEERING LABMMRY AQUATIC TOXICIIY DATA SHEET ATRACIDOC k3, Type Obterial Tested YV\ Test Organisav3@*", Test SolutionVol@IM-til )ra AvS. Wt. Aran Avg. Size 4& co No. Orsanisms per Time Start*d!!QQL"t@- Date started Dilution W&tsr CalbaR FI-Iter- Well Kate @-(TOW Date covieted Li 0 Investigator@,.@. IC 60 (9Sl C00fid4mce Limits) we o@NNo.0 go@is2mFN )rganism U24L Nrs, 0.0. 9-RIAiLlAdI---RM ;ontroii .4 W pl@,0 46 lin. D.o. n iin. 0.0. US OH @ No. ft_&A IIH I -t-7 7-b o 1.1 7.e4 96 Hrs Dtoi!d o 1.2 0.0. 12- 7.2 va. Te!!E. Dwmwnts: l@k0 C6 2-C-) L I RUh rr%90D IT. D2 0 MRSDAY9, POY llp 1979 l5t34251 L. 44 1-5*1 EHYIRCPWC-*TAL WSEFNK* LFW. - VAUT* OOOPMMIT FOMYSIS FCW RIDWSOW D*Tp4oo-o- "a OF CURYE-S ? I "C - OF CEM. FOR CUW#* I - 7 4 I"PUT DFVTO-CMM.9,ND. EMOM90C..@ 7 650920*0 7 lOOOp2Op4 7 155OP20PI2 7.240OP20920 uuu 1000 1550 2400 'CHI-SO LOG CCW. '2.St2913 3 3.190332 3.390211 PRGUMILITY IS > FRMT 110 RESPOWING 0 0.2 0.6 1 0.05 FITTED FPMTTU* 0- OOWM0574 0:14"e9* 0.6909-147 0.%757905 "a OF ITEROTIOM I"TERGOOT (OV) SLUM 6 -2+.7904-. 7.917981 (''":TER066001ETY FRCTD* -LC-O 1351.882 95 PCT. LOWER COW. LIPOIT m 95 PCT. UPPER COW. LIPOIT u CHI so FOR Llt*JWITY CM SO FEW PmowrLITY 1.61074. 0. 26 6 - 0.1348014 1193.531 1533.091