Document g2kRdpgZXVRoZqy9rpbm0BGrQ

Corporate Health Physics Corporate Occupational Medicine Corporate Product Responsibility Corporate Toxicology 3M Medical Department 3M Center, 220-2E-02 PO Box 33220 St. Paul, MN 55133-3220 651 733 1110 AfUX-Q Analysis of Selected Decatur Employee Serum for Sulfonic and Carboxylic Fluorochemicals This 3M technical report analyzed the 5 highest organic fluorine containing Decatur employee samples and analyzed the serum for perfluorooctanesulfonate, perfluorohexanesulfonate and perfluorooctanoate. In decreasing percent found, perfluorooctanesulfonate contributed between 55 - 80%, perfluorooctanoate contributed between 15 - 30% and perfluorohexanesulfonate contributed 5- 15% of the organic fluorine. Analytical methods used were electron capture and microwave plasma detector. 001709 3M TECHNICAL REPORT Report Number: 7230 Date: October 4, 1979 TITLE: Analysis of Selected Decatur Employee Serum for Sulfonic and Carboxylic Fluorochemicals AUTHOR: ABSTRACT: The precence of C_ and CQ sulfonic acids was detected in certain blood samples using both electron capture and microwave plasma detector methods. 001710 t*4*U CENTRAL ANALYTICAL LABORATORY Report N o .--------- 1140.--------------- Da t e . - - - - - . O c t o b e r _ 4 L _1979_ _ _ _ _ l>^v'#5i*ir i*.***.- J ,,*; Subject: Characterization of Decatur RF Values Requestor:_____ -........... Dept. N a m e _CojBierial _C^micalsproj. N o . - 2 U 2-U PP Request N o ____ A7362?_...... Dated - October _3,_I2Z..... R eport: The serum of 10 selected 3M Decatur employees was submitted (A72754) in June, 1979 for analysis of organic fluoride levels and the total organic fluoride values were reported in Analytical Report 7194. Subsequent to that work, it became of interest to further characterize the fluoride eerum value and attempt to identify the presence of 3M fluorochemicals. Initial analysis (7/18/79) by the microwave plasma detector (KPD) established the presence of the expected perfluoro^ctanesulfonate and perfluorooctanoate anions in the serum extract of -25 and . -22. However, the observation of perfluorohexanesulfonate anion in the above extracts was unexpected and it therefore became necessary to spend additional analytical development time In order to measure these 3 fluorochemicals in the same serum extract sample. During this time, the method was evaluated on the electron capture (EC) gas chromatograph. The EC detector would be expected to have greater sensitivity than the MPD for these fluorochemicals but with less specificity since the MPD can be operated on the fluorine channel eliminating any confusion by other EC active compounds. We selected the 5 highest fluorine containing Decatur samples and analyzed the serum extract for perfluorohexanesulfonate, perfluorooctanesulfonate, and perfluorooctanoate anion. We used both the MPD and EC detection systems and report the following values: % FOUNlP Person -22 -23 -24 -2P -26 RF (ppm) 10.1 5.7 9.4 11.8 4.1 Perfluorohex anesulfonate 53 15% 5% 5% 10% Perfluorooc tanesulfonate 60% 70% 80% 55% 65% Perfluoro octanoate 30% 20% 15% 20% 25% Total 95% 105% 100% 80% 100% The analysis was conducted on a single determination (1 ml of serum) due to limited availability of sample and time and represents our interpretation of the average of the EC and MPD values. No internal standard was used; expect 110-203 accuracy. AR 6962, 7028, 7194 P r e v i o u s l y identified as -21(Z) due to mis-labeling by medical personnel. 001711 . No. 7230 Octobe r A, 1979 ?age 2 } %t*> *., j ! ,** .- i , { !J i ' .. v - S i ' : , ' ! The above results are necessarily somewhat approximate due to the lack of an Internal standard and continual refinements In the derlvatisatlon and GC of the corresponding methyl ester. Sufficient sample remains for additional analysis. Further improvemen in the analysis are and will continue to ke made as time permits. He thought it best to report these values now, especially since we Identified the presence of perfluorohexanesulfonate in human serum extract and no toxicology studies to date have been completed on this fluorochemical. Three years ago (10-18-76) ve reported Chat Decatur employee . serum extract contained perfluorooctanasulfonate (based on F/NMR) and a minor fluorine containing component now Identified as perfluorooctanoate. Therefore, the presence of per- fluorooctanesulfonate In this Individual has been known for some time. How, with improved analytical methods, we have greater sensitivity and can determine the presence of perfluorohexanesulfonate in addition to perfluorooctanasulfonate anion. 001712