Document g04NQR8xVbwp5wg6xnq4V99Q

AR. - 0 7 & f ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE________________________________________________ Identity: Mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC203CF. Current information indicates it is a mixture of 1% PFOS, 20% diethylene glycol monobutyl ether, 70.2% water, 2.75% Acrylic foamer, 3% sodium octyl sulfate, 1% triethanolamine, 2% sulfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity o f the fluorochemical component of the test sample. METHOD:_________________________________________________________ Method: OECD Guideline 202 Type: Acute static GLP: No Year completed: 1991 Species: Daphnia magna Supplier: In-house cultures maintained at EnviroSystems Divisio, Resource Analysts, Inc., Hampton, NH. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: No calculations performed, based on study results. Test conditions: Dilution water: Filtered natural well water Dilution water chemistry: Conductivity: 840 pmhos/cm pH: 7.3 Lighting: Cool-white fluorescent lights at 20 pEin/sec/m2. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A primary 1000 mg/L stock solution was prepared and added directly to create test solutions. Exposure vessels: 250 mL beakers containing 200 mL exposure solution at a water depth of approximately 6 cm. Number of replicates: 4 Number of daphnids per replicate: 5 001234 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-48 hours): 7.3 - 8.2 (control exposure) 7.5 - 8.3 (1000 mg/L exposure) Temperature range (0-48 hours): 1 9 . 1 - 19.6 C (control exposure) 1 9 .1 - 19.6 C (1000 mg/L exposure) Conductivity range (0-48 hours): 840 - 860 pmhos/cm (control exposure) 860 - 870 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-48 hours): 8.3 - 9.2 mg/L (control exposure) 8.3 - 9.2 mg/L (1000 mg/L exposure) Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mg/L. Element values: 48-hour EC5o = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________ ________________ The test substance 48-hour EC5owas determined to be >1000 mg/L. No mortality or sublethal effects were observed. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________ ________________________ This test was conducted by EnviroSystems Division, Resource Analysts, Incorporated, of Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J1884-3,1991. 001235 O TH ER _______________ Last changed: 6/28/00. 001236 BEST COPY AVAILABLE Stud? Title Static Acute Toxicity of FC-203CF to the Dephnid, Dapbnia aagna Author Timothy J. Ward Robert L. Boeri b e s t c o p y a v a il a b l e I. SCHHAHY The cut* toxicity of PC-203CY to the daphnid, Dtphal* napna, is described in this final report. The test was conducted for 3H Company for 48 hours during June 12 to 14, 1991, at the EnviroSystems Division of Resource Analysts, Inc. in Hampton, Hew Hampshire. It was conducted by Jeanne Hagazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Timothy Hard. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20 t 1C. The dilution water was filtered natural well water collected at Hampton, Rev Hampshire. Reration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Honinal concentrations of FC-203Cr were: 0 mg/t. (control), 150 ng/L, 250 ng/1, 400 mg/L, 600 mg/I>, and 1,000 mg/I>. Nominal concentrations were used for all calculations. Daphnids used in the test were produced from an in-bouse culture and were less than 24 hours old at the start of the test. After 48 hours of exposure the control daphnids had an average wet weight (blotted dry) of 0.0008 g, resulting in a loading rate of 0.02 g/L. All daphnids were in good condition at the beginning of the study. The test substance was not toxic to daphnids at tested concentrations. Exposure of daphnids to the test substance resulted in t 41. hour EC50 greater than 1,000 mg/L FC-203CT. Ho sublethal effect was observed in any test vessel during the test. BEST COPY AVAILABLE x x . o?table contents SECTION: I. Susaary IX. ' Table ot Contents III. Xndx ot Tables and Figures IV. Introduction V. Methods and Materials VI. -. :Xesults VII. ^ `References PAGE 2 3 4 5 5 S 11 BEST COPY AVAILABLE III. IMDEX Or TABLES AMD FIGURES i Table 1. Chemical characterization of a representative sample of natural well water used as dilution water for toxicity test Table 2. Survival data from toxicity test Table 3. Median effective concentrations (EC50s) from toxicity test Table A.l. Conductivity, pH, temperature, and dissolved oxygen concentration measured during toxicity test PAGE 6 9 10 13 & BEST COPY AVAILABLE 17. OTIOMCTXO S' This study i u sponsored by 3M Company, St. Paul, Minnesota. The objective of the study was to determine the acute toxicity of rc-203CF to the daphnid, a freshwater invertebrate. The report contains sections that describe the methods and materials employed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. METHODS AND MATERIALS TEST SUBSTANCE: FC-203CF (EnviroSystems Sample Humber 2937E) was delivered to EnviroSystems on May 23, 1991. It was contained in a 40 oz plastic bottle that was labelled with the following information: "J1884-3, FC-203CF". FC-203CF (an orange-brown liquid) was supplied by 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample (approximately 1.0 gram) will be retained at EnviroSystems for a minimum of 10 years. DILUTIOH VATKJU Rater used for acclimation of test organisms and for all toxicity testing was collected from wells at EnviroSystems in Hampton, Mew Hampshire. Hater was stored in 500-gallon polyethylene tanks where it was aerated. Results of chemical analysis of a representative sample of water are presented in Table 1. TEST ORGAMISH: *-Vrf V Juvenile daphnids employed as test ^ organisms were from a .single source - and were identified using an appropriate taxonomic key. Daphnids /used -in the test!wereMproduced from an in-house culture-fand were less than -"i24 hours old at;th'eistart.of the test. Control daphnids were weighed at the ^conclusion off?the|$toxicity;, test.' .Prior to testing,' daphnids were ^ i n t a i n ^ ^ in ;100%^dilution water under static conditions' in' 4 liter glass |jars.^~iDttringij^acclimation daphnids were not treated for disease and they |were^.freei^'oIfmp..puen^iicknessl* injuries,i|and abnormalities/at; the ^beginningw'jftv'o--f/.it_he^--te_s--t^iD--uri--ng* --the a-c-c-l-i-mattiicon perit&iRdhourmprior to i-the^test''ffiiinitiationSth^esi^atwe.was./l?j5*C^|Daphni'dsywere -fedvyeast,trout^chowv,,0and/orfethe@freshwater'^alga*SeIenastrM ,capricorhutum once or twice daily/ i.'Ai'lW" ,/r ^ 4 W0S& [firi' i (Mujppa *.1 I k,'fmif*I 0012411 v;, ^ 8 ^ ;.-. : | r BEST COPY AVAILABLE "1 - V V" - Table 1. Chemical characterization of a representative aampla of natural wall watar used aa dilution water for toxicity teat Paraeeter PH Conductivity Organochlorine peaticides Organophosphorus pesticides Polychlorinated biphenyls Unit of Keasureaent Reporting Lieit pH units ~ uuhos/ca -- ug/Ii 0.5 ug/L 2 ug/L 1 Measured Value 7.3 840 HD HD HD v icS Rotea: . 1.: HD- not detected at or above the reporting Unit. 2. pH, hardness, and conductivity were neaaured in dilution : water collected fro the control,teat vessel prior to - introduction of organises and.pesticide and PCB data is collected during-'frtoutine -bian/nualV watTer qualAity\tes!ting..'i,.T, r `y m W *:. Vi$V - "'.km i ? . # # ... Llrx^SutakiaryolPi - <Wv BEST COPY AVAILABLE TOXICITT TISTIXC: X screening test with the test substance was conducted during Hay 29 to 31, 1991. Hoainal concantrations of teat substance ware 1.0 mg/L, 10 ag/L, 100 ag/L, and 1,000 ag/L. After 48 houra of exposure there was 20% survival at 1,000 ag/L, and 80% survival or greater at 1.0 ng/L, 10 ag/L, and 100 ag/L. Surviving daphnids exposed to 100 ag/L at 48 houra and 1,000 ag/L at 24 to 48 hours era inaobilized. The definitive toxicity test was performed during June 12 to 14, 1991. The test vas conducted at a target taaparature of 20 t 1C with five concentrations of test substance and a dilution water control. X 1,000 ag/L stock solution was formulated' without the use of a solvent. Hoainal concentrations of the test aaterial were: 0 ag/L (control), 150 ag/L, 250 ag/L, 400 ag/L, 600 ag/L, and 1,000 ag/L. Twenty daphnida were randomly and equally distributed aaong four replicates of each treataent. The test was performed in 250 al glass beakers that contained 200 al of test solution (water depth was approximately 6 cm). Test vessels were randomly arranged in an incubator during the 48 hour test (a random numbers table was used to select the location of each vessel). X 16 hour light and 8 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provided a light intensity of 20 uEs-1m-*. Aeration was not required to maintain dissolved oxygen concentrations above acceptable levels. Daphnids were not fed during the test. The nuaber of surviving organisms and the occurrence of immobilization (inability to swim within 15 seconds after gentle agitation) or other sublethal effects (loss of equilibrium, erratic swiaaing, less of reflex, excitability, discoloration, or change in behavior) were determined visually and recorded initially and after 24 and 48 hours. Dissolved oxygen (YSX Hodel 57 aeter; instrument nuaber PRL-3) ,>'pH (Beckman model pBX 12 aeter;;instrument number PRL-51), conductivity (Cole Farmer nuaber ..1481-60,; instrument nuaber PRL-50), and teaperature (XSTH mercury thermometer; thermometer nuaber 2265) were measured and recorded' daily in each test chamber that contained ;live animals.'^, ' .v-. V/ > ''-r--' S T X T X ^ ^ ^ f e r H O D S : V V ' r-' ,* -' . V ?.*&*&*. ' '' Results of the toxicity .test^,,could "not be interpreted by standard .^statistical techniques (Stephan)- 1983) because 50% survival or greater'occurred at all tested concentrations.^ ... i*>.., V' Vi fesfii *' * ./V A fra t/A , h q . SubWdtary o f MUJPORE -4 00124. b e s t c o p y a v a il a b l e /`A TO. BSSBliTS All test vessels containing TC-203CT vara initially claar and remained clear throughout the teat. Biological and water quality data generated by the acute toxicity teat are presented in Table 2 and Appendix A, respectively. 'One hundred percent survival occurred in the control exposure. Control daphnida had an average wet weight (blotted dry) of 0.0008 g ' at the end .of the teat. Loading rate during the toxicity test was approximately 0.02 g/l>. The teat substance was not toxic to daphnida at tested concentrations. The 24 and -;'48 hour EC50s'for daphnida exposed to FC-203CT are presented in ' Table 3. Exposure of daphnida to TC-203C? resulted in a 48 hour LC50 greater than 1,000 ng/L. Xo sublethal effect was observed in any teat: vessel during the test. BEST COPY AVAILABLE Tbl 2. Survival data Iron toxicity teat Nominal Concentration (ag/Ii) rep. 0 (control) 1 ; 150 250 400 4 " j^t ,` vM-.-if 600 *:.*i . #-if,.: '^21 vV'3s s&rcS - M r .JVA&lS Ruaber Alive 0 hr 24 hr 48 hr Nimbar Affected Ohr 24hr 48hr <f.Av- 000 000 000 -0 0 0 000 00 0 000 00 0 000 00 0 000 00 0 000 000 00 0 00 0 0 v;i 0 i 0 -. >?t'0 . sSgfc." +! ? ; " t* 0 0 0 0 *(': o V,,0 ' H R p & S & g o ; : ? o .- MBiatO::$&&&<># m s * ^ j ^ ^ Ufi4ftMili^tgwia'ifithin 15 * 001245 ` s* BEST COPY AVAILABLE Tabi* 3. Badila affactira concantrationa (KC50a) froa tozicity tait Izpoaura pariod EC50 95 parcant confidane# liaita Calculation aethod 24 hoara >1,000 ag/I 48 houra >1,000 ag/L -- -- -- -- - ='f. .- 'rii' ' . T- ..*r-.1!* .1 .. IY.*;i |v fg g iift Jv V-v", . 44 4 , KV .-/'.*>~i -, f i <S.;,V. S ; l;. .... r- `.CTSa^';--;<rr.,V.; f -Z' v.rA .. '-^I -'.V7? * 4 '11. ^-C" - 11r 1:t;4 P --'- Il* f e ' i v CIMUK5R6 .;,, M *l 4 ;`- BEST COPY AVAILABLE Tabl* 8.1. Conductivity, pH, tanparatura, and diasolvad oxygan concantration aaaaurad daring toxicity taat Nominal ncantrat:Lon (ag/L) Rap. Conductivity (uaho/ca) 0 24 48 hr hr hr 0 (control)1 1 840 860 2 840 850 3 840 850 4 840 860 150 1 850 840 2 850 840 3 850 840 4 850 840 250 1 850 850 2 850 850 3 850 850 4 850 850 400 .SST'600: 1 V 850 2 . 850 . 3 'V 850 4 -.V 850 - ^v .V i - 850 860 860 860 860 870 870 870 870 |860> 860 860 860 860 830 830 840 840 840 850 850 850 870 870 870 870 870 870 .870 pH 0 24 48 hr hr hr 7.3 7.8 8.0 7.3 7.9 8.1 7.3 7.9 8.2 7.3 7.9 8.2 7.3 8.0 8.2 7.3 8.0 8.2 7.3 8.0 8.2 7.3 8.0 8.2 7.4 8.1 8.2 7.4 8.1 8.2 7.4 8.1 8.2 7.4 8.1 8.3 7.5 8.1 8.3 7.5 8.1 8.3 7.5 8.1 8.3 7.5 8.1 8.3 7.5 ; 8.1 7.5 ~ ' 8.1 7.5 8.1 7 -5 -$~'8.i 8.3 eil8!3 ' -*v 8 .0 7.5 -7.5ii:#^8.0^ i,8'.3' - f8'f3gr' r|8 |3 "f; * 8:3 */ > / .[vt` S'j,y '.; P ^- !jvi'--' 00124! '**:V I